LOCUS       DATAIX010000001         1594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_148947, whole genome shotgun sequence.
ACCESSION   DATAIX010000001 DATAIX010000000
VERSION     DATAIX010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148947"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VHM65_00005"
     CDS             <1..188
                     /locus_tag="VHM65_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704116.1"
                     /translation="PMEDIVCGSCGRTPGESDRDQARARLAWARSVENSRTVWTCDRC
                     ARTHLRSIEGKLDSTWW"
     gene            complement(196..1401)
                     /locus_tag="VHM65_00010"
     CDS             complement(196..1401)
                     /locus_tag="VHM65_00010"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX2704117.1"
                     /translation="MQARTAEQLFAVLGELKGGAMKFGQALSIFESALPEELAAPYRE
                     TLTRLQDSAPPLPADRVHGVLTVELGVDWRDHFASFDDTPIAAASIGQVHRAIWSDGR
                     EVAVKIQYPGAGKALLGDLNQLSRVARMATSWIPGIDIKPILEELRNRMSEELDYRIE
                     AYYQETFAEGFEGDPDFLIPHVLAFSERILVSEWVAGRALSELIASGTQDERDEAAGH
                     YIEFLLAGPARVGLLHADPHPGNFKLLPDGRLAIVDFGACNRLPDGMPPDMGELLTMA
                     VAYDSEGVVDRLREIGFVKSTIDLEADRLLEYLEPFIAPVRTRTFRFTRPWLRGVFAQ
                     INDPRRPTYTMALKLNLPPEYLLIHRVWIGGIGVLCQLGGTVRGPEAVNRYIPGAALP
                     EPANPDDEV"
BASE COUNT          232 a          538 c          591 g          233 t
ORIGIN      
        1 gtccgatgga ggacatcgtg tgtgggtcct gcggacggac ccctggcgag agcgaccgag
       61 accaggcccg cgctcggctc gcgtgggccc gcagcgtcga gaacagccgg accgtctgga
      121 cctgcgaccg gtgcgcccgg acccatctgc gcagcatcga aggcaagctc gactccacgt
      181 ggtggtgagc gcgcgtcata cctcgtcgtc ggggttggcg ggctcgggga gcgcggcacc
      241 ggggatgtag cggttgaccg cctccgggcc gcgcacggtg ccaccgagct ggcacagtac
      301 cccgatgccg ccgatccaga cgcggtggat gaggaggtac tccggaggca ggttgagctt
      361 gagagccatc gtgtatgtcg gtcgccgcgg gtcgttgatc tgggcaaaga ccccccgcag
      421 ccacgggcgg gtgaaccgga atgtccgagt ccgcacgggc gcgatgaacg gctcgaggta
      481 ctccaggagc cggtcggcct cgaggtcgat cgtcgacttg acgaagccga tctctcgcag
      541 ccggtcgacc acgccctcgc tgtcgtaggc caccgccatc gtcaggagct cacccatgtc
      601 cgggggcatc ccgtcgggca gccggttgca cgcgccgaag tcgacgatcg cgagccgccc
      661 gtcgggcagg agcttgaagt tgccggggtg ggggtcggcg tggagcagcc cgacccgggc
      721 cgggccggcg aggaggaact cgatgtagtg gcccgcggcc tcgtcgcgct cgtcctgagt
      781 gccgctggcg atgagctcgg acagggccct gcccgcgacc cattcgctga cgaggatgcg
      841 ctcgctgaag gcgagcacat gggggatgag gaagtcgggg tcgccctcga acccctcggc
      901 gaacgtctcc tggtagtacg cctcgatccg gtagtcgagc tcctcgctca tccggttgcg
      961 cagctcttcc aggatcggct tgatgtcgat cccggggatc cacgacgtcg ccatccgggc
     1021 gacccgcgag agctggttga ggtcaccgag aagggccttg cccgcgccgg ggtactggat
     1081 cttgaccgcg acctcgcgcc cgtcggacca gatcgcgcgg tgcacctgcc cgatcgaggc
     1141 cgcagcgatg ggagtgtcgt cgaaggacgc gaaatggtcg cgccagtcca cgcccagctc
     1201 gacggtgaga acgccgtgga cccggtctgc gggcagcggc ggtgcgctgt cctggagcct
     1261 ggtcaacgtc tcgcggtatg gcgcggccag ctcctcgggg agcgcggact cgaagatcga
     1321 cagggcctgg ccgaacttca tcgcccctcc cttgagctcg ccgagcacgg cgaagagctg
     1381 ctcggcggtg cgtgcctgca tctcggcggc gacggcctcg gccgggcggc ctccgacccg
     1441 cttgctgagg ccgagagcgg cccgccccgc gataccgacg ggccgccaag ctggccaggc
     1501 tgcccgtcgg tatcgcgggg cgggccgctc tcggcctcgg ctagcgggtc ggaggccgcc
     1561 cggccgaggc cgtcgccgcc gagatgcagg cacg
//
LOCUS       DATAIX010000002         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_29868, whole genome shotgun sequence.
ACCESSION   DATAIX010000002 DATAIX010000000
VERSION     DATAIX010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29868"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1596)
                     /locus_tag="VHM65_00015"
     CDS             complement(<1..1596)
                     /locus_tag="VHM65_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704118.1"
                     /translation="MTPGRPRSRRRPCRVLVVAAAGYGKTASAEADLADDGLRCSAAQ
                     VLALGTPVSRPLVVEDLHQVCAGERVEVLQRLAALADSVPVTITSREPLDRTSRGMLR
                     GQVFERGPGDLALTGPAIARVLADEYGIADPEAPARIHALTRGWPALVHFAADALSRR
                     PGDDLAAALTRPASAAAAWLQNDVLSTLPGDVAGLLSALADLDPLTEDLVGRRLGGHG
                     PRPEGSAFAWLCRVGLLVPHPRLDLLGREGFSVVPAVAMVLTRARAGAATDPARLVSA
                     ARWYEHHGHPFAAADAYARAGDGARAMSLVDARGAEMIAHGDAPGIVTLIGRAEAGSV
                     TLASSRINAEALHMSGDSTAAQRAYAPLAAAADLDGWDPGLAHRLAAVHHTQGTLRAA
                     LDVLDRVPPGTLTTDSDGIHWRACRVNVLSMLGHDEQARDLAASTLALAEHATDPRSL
                     TAAHQAMAKTSSGARKQAHLGLALAAAKQAGDVVSAARILGNQSFVLLAAASYTEAVG
                     VSREAVRAAEIARPTGALIAALHN"
     gene            complement(1718..>2719)
                     /locus_tag="VHM65_00020"
     CDS             complement(1718..>2719)
                     /locus_tag="VHM65_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2704119.1"
                     /translation="PADVVASLDAAGTPAGFDVRYVTRADEEAVRLAVRDGDATAGLA
                     GPRLYTSSRAEGPFPTVVAQTVVALETSRRMAAAGLRPEQIADLQSVRPPEQVTVGRV
                     DDEGRVIAGIAVGIVLYLALTVAGSAIATTVAMEKSTRISEVLLAVLRPSQVLVGTVA
                     AVGTATLVQLLLLAAPLAVAVRVTDRIGLPAVAAGDIGLAVAWFVLGFVLYAFLFAAT
                     SALVDKVTEVNSAILPVTLILIGAYLLAVTVVQMDPGSGWSVAASMFPLTAPLAMPIR
                     WASGEVPVVELVLAMALTAATAALLVVVASSIYRRALLITGRRVRLREVIGRSARQG"
BASE COUNT          390 a          935 c         1066 g          328 t
ORIGIN      
        1 gttgtgcagg gcggcgatga gggcccccgt gggccgggcg atctcggcag ccctcaccgc
       61 ctcgcgcgac accccgaccg cctcggtgta ggacgcggct gcgagcagga cgaacgactg
      121 gttcccgagg attctcgccg ccgagacgac atcgccggcc tgcttggcgg ccgcgagtgc
      181 caggcccagg tgggcctgct tgcgtgcgcc cgagctcgtc ttggccatcg cctggtgcgc
      241 ggccgtcagc gagcgggggt ctgtggcgtg ctcggccagg gcgagggtcg acgcggcgag
      301 gtcccgcgcc tgctcgtcgt ggccgagcat cgacaggacg ttgacgcggc aggctcgcca
      361 gtggatgccg tcggagtcgg tggtgagcgt gccgggcggc acccggtcga ggacgtcgag
      421 ggcggcgcgc agggtgcctt gggtgtgatg gaccgccgcg agccgatgag ccaggcccgg
      481 gtcccatccg tcgaggtccg cggccgcggc cagcggtgcg taggcgcgtt gagcggcagt
      541 cgagtcgccc gacatgtgca gcgcttcggc gttgatccgc gaggaggcga gggtgaccga
      601 cccggcctcc gccctcccga tgagcgtgac gatcccgggg gcgtcgccgt gggcgatcat
      661 ctcggcgccc cgagcgtcga cgagcgacat ggcgcgggcg ccgtcgcccg cgcgggcgta
      721 ggcatcggcg gccgcgaacg ggtgtccgtg gtgctcgtac caccgggcgg cgctcaccag
      781 gcgggccggg tcggtcgccg cgccagcccg ggctcgggtc agcaccatcg cgacagccgg
      841 tacgaccgag aagccctcgc gcccgagcag gtcgaggcgt ggatgcggca cgagcagccc
      901 cacgcggcag agccacgcga aggcgctccc ctccggtctc gggccgtggc cgccgaggcg
      961 tcggccgacg aggtcctcgg tcagtgggtc gaggtcggcg agagcggaca gcagcccggc
     1021 gacgtcgccc gggagcgtcg agaggacgtc gttctggagc cacgcggccg cggccgaggc
     1081 cggccgggtc agggcggcgg cgaggtcgtc acccggcctg cgggacaggg cgtcggcggc
     1141 gaagtgcacg agcgccggcc agccgcgggt gagggcatgg atccgagccg gcgcctcggg
     1201 gtcggcgatg ccgtactcgt cggcgaggac ccgggcaatc gcgggcccag taagggccag
     1261 gtcgcctgga ccgcgctcga agacctgccc ccgaagcatg cctcgactcg tgcggtccag
     1321 cggctcccgc gaggtgatgg tcacggggac cgagtcggcg agggcagcca ggcgctggag
     1381 cacctcgacc cgctcgccgg cgcagacctg gtgcaggtcc tcgacgacga gggggcggga
     1441 cactggtgtg ccgagggcga gcacctgggc ggcactgcag cgcaggccgt cgtcggccag
     1501 atcggcctcg gcggaggcgg tcttgccgta cccagcggca gcgacgacga ggacccggca
     1561 gggccgccgc cggcttctcg gccggccggg cgtcatacag gaagaatccg ggcgtggctc
     1621 cggtgtgcag tcatagtgtc aagagcgtcg cccgaagcgg ggtgatacat cgcgcgcccg
     1681 gacactccac ttcggatgtc gctcgcctca gggactgtca tccttgccgg gcgcttcggc
     1741 cgatcacctc gcgcagccgc acccgtcgcc cggtgatgag cagcgcccgc cggtagatcg
     1801 aggaggccac cacgaccaac agcgctgcgg tggcagcggt gagcgccatg gcgagcacga
     1861 gctcgacgac ggggacctcg ccggaggccc accggatcgg catcgccagc ggtgcggtga
     1921 gcgggaacat cgacgccgcg acgctccacc cgctccccgg gtccatctgg acgaccgtga
     1981 cggcgagaag gtaggcaccg atgaggatga gcgtgaccgg caggatcgcc gagttcacct
     2041 cggtgacctt gtcgacgagg gccgaggtgg ccgcgaacag gaacgcatag agcacgaagc
     2101 cgaggacgaa ccacgcgacg gccagcccga tgtcgcccgc tgcgacggcc ggcagcccga
     2161 tccggtcggt gacccgtacg gcgacagcca gcggtgcggc gagcaggagc agctggacca
     2221 gggtggccgt gccaaccgcg gcgacggtgc cgacgagaac ctggctgggg cgcaggacgg
     2281 ccagcagcac ctcagagatg cgggtggact tctccatggc gacggtggtg gcgatcgcgc
     2341 tgccggcgac ggtgagcgcc aggtagagga cgatcccgac ggcgatcccg gcgatgacgc
     2401 gcccctcatc gtccacccgt ccgacggtga cctgctcggg cgggcgcacc gactggaggt
     2461 ccgcgatctg ctccgggcgc agccctgcag cggccatccg gcgcgacgtc tccagcgcca
     2521 cgaccgtctg ggccaccacg gtggggaagg ggccttctgc ccgtgatgac gtgtagagcc
     2581 tgggccccgc gagcccggcg gtggcgtcac cgtcgcggac ggcgaggcgg accgcctcct
     2641 cgtcggcgcg ggtcacatac ctgacatcga agccggcggg cgtcccagcc gcgtcgaggg
     2701 acgccacgac gtctgcggg
//
LOCUS       DATAIX010000003         2398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_173, whole genome shotgun sequence.
ACCESSION   DATAIX010000003 DATAIX010000000
VERSION     DATAIX010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VHM65_00025"
     CDS             <1..459
                     /locus_tag="VHM65_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704120.1"
                     /translation="VGETPLWSSFVWRNEVVDAFVEIVAAPWFAWAATGTPVLNVWLR
                     ALGARIGRGVWCESYWLPEADLVTLGDGASVNRGCVLQTHLFHDRIMAIDTVELAAGS
                     TMGPHGVILPAASIGANTTVGPVSLVLRGERVPESSRWVGNPIGPWPHGR"
     gene            449..1768
                     /locus_tag="VHM65_00030"
     CDS             449..1768
                     /locus_tag="VHM65_00030"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX2704121.1"
                     /translation="MDADPYLPGHGDVTWGATSYQLELDYRMSSNRLAASARITARAH
                     TDLRTVTLDLAAPLRVSKVLVDGRKPRKYAHRANRLVLTLAKRVDEGAAFTIEIGYGG
                     NPGPLRGTWGEVGWEELSNGVIVAGQPNGAPTWFPCNDRPADKAPYRFEVTADSPYTV
                     RANGVLRGRRIRGSRTTWTFEQAEPMATYLATLQLGSYETIRLPSPGSAVAQWALLPP
                     RLRTAFDVDFGRQDAMMVLFERRFGRYPFAEYTVVVTEDPLEIPLEAQGLAVFGADFV
                     SGRRREERLVAHELAHQWFGNSLTVAGWGHIWLNEGFACYAEWLWWEACGDRTAEASA
                     RQHYRRLSGLAQDYAIADPGPDLMFDDRTYKRGALTLHALRVHLGDKAFFELLRSWVS
                     EHAHGVVTTADFVAHAQRHAARSGRPSVRVLLRRWLYDAALPSYPRA"
     gene            complement(1786..>2398)
                     /locus_tag="VHM65_00035"
     CDS             complement(1786..>2398)
                     /locus_tag="VHM65_00035"
                     /inference="COORDINATES: protein motif:HMM:NF045214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704122.1"
                     /translation="DTEGWVFDGFIRTSGLIEASYFNAYVAENRQYDGYDASLKTAYN
                     FGFLDTRPDWVEHFRYQNGLLISYWDASVNDNNVGDHPGSGLVLPIDAHPALKHWSDG
                     TLLRPRLLSADSTFGLAPTDRMVVHQNGVAAVIPSKPAVPRFDDTRTWWTNADAHGAT
                     GSHPGRYQPGWSGVDPPKTGTVITVVAAAHGSNTLTVTVAPKR"
BASE COUNT          324 a          861 c          852 g          361 t
ORIGIN      
        1 gtcggggaga ccccgttgtg gagctcgttc gtctggcgca acgaggtcgt cgacgccttc
       61 gtcgagatcg tcgccgcgcc gtggttcgcc tgggcggcca ccgggacccc ggtcctcaac
      121 gtgtggctcc gcgccctggg cgcgaggatc ggccgcggcg tctggtgcga gtcgtactgg
      181 ctgcccgagg ccgacctggt caccctcggc gacggcgcga gcgtcaaccg cggctgcgtc
      241 ctgcagaccc acctcttcca cgaccggatc atggccatcg acaccgtcga gctcgccgcg
      301 ggctcgacga tgggccccca tggagtcata ctccccgcag cctcgatcgg cgccaacacc
      361 acggtcggcc cggtctcgct cgtcctgcgc ggcgagcggg tgcccgagtc gagccgctgg
      421 gtcggcaacc cgatcgggcc gtggccccat ggacgctgac ccctacctgc ccggccacgg
      481 cgacgtgacg tggggggcga cgtcatacca gctcgaactt gactatcgga tgtcgagcaa
      541 ccggctcgcc gccagcgccc ggatcaccgc ccgcgcccat accgacctgc gcacggtgac
      601 cctcgacctg gccgcgccac tgcgggtcag caaggtcctc gtcgacggcc gcaaaccgcg
      661 caagtacgcc caccgcgcga accgcctcgt cctcacgctc gccaagcggg tcgacgaggg
      721 tgcggccttc acgatcgaga tcgggtatgg cggcaacccc ggcccgctgc gcggcacgtg
      781 gggggaggtc ggctgggagg agctgagcaa cggggtcatc gtcgcggggc agcccaacgg
      841 cgcaccgacg tggttcccgt gcaacgaccg gccggccgac aaggcgccct accgcttcga
      901 ggtgaccgcc gactcgccct acacggtccg cgccaacggg gtgctgcgcg ggcggcggat
      961 ccgcggcagc cgcaccacct ggaccttcga gcaggccgag ccgatggcga cctacctcgc
     1021 cacgctgcag ctcggcagct acgagacgat ccggctgccg agcccgggct ccgccgtggc
     1081 gcagtgggcg ctgctgccgc ctcggctgcg cacggccttc gacgtcgact tcggccggca
     1141 ggacgcgatg atggtgctgt tcgagaggcg cttcggacgt tacccgttcg cggagtacac
     1201 cgtcgtcgtc accgaggacc cgctcgagat cccgctcgag gcgcaggggc tcgccgtctt
     1261 cggtgccgac ttcgtcagcg gccgccgccg cgaggagcgc cttgtcgcgc acgagctggc
     1321 ccaccagtgg ttcggcaact cgctcaccgt cgccggatgg ggccacatct ggctcaacga
     1381 gggcttcgcc tgctatgccg agtggctgtg gtgggaggcc tgtggtgatc ggacggccga
     1441 ggccagcgcc cgtcagcact accggcgtct ttccggcctg gcgcaggact atgccatcgc
     1501 cgaccccggg ccggacctca tgttcgacga ccggacctac aagcgcggcg cgctgaccct
     1561 gcacgccctg cgggttcatc tcggcgacaa ggcattcttc gagctgctgc gctcctgggt
     1621 gtccgagcac gctcatgggg tggtgacgac ggccgacttc gtcgcgcacg cccagcgcca
     1681 cgccgcgcgg tcgggacggc cctcggtgcg ggtcctcctg cgccgctggc tgtatgacgc
     1741 ggcgctgccg tcctacccgc gcgcctgacg gatcggtccg gactgctagc gcttcggcgc
     1801 caccgtgacg gtgagggtgt tcgagccgtg ggccgccgcg accaccgtga tgaccgtgcc
     1861 ggtcttcggc gggtcgacgc cggaccagcc cggctggtag cgcccggggt gcgagccggt
     1921 ggcgccgtgg gcgtccgcgt tggtccacca ggtccgggtg tcgtcgaagc gcggcacggc
     1981 cggcttggac gggatcaccg ccgcgacgcc gttctggtgc acgaccatcc ggtccgtcgg
     2041 cgcgaggccg aacgtcgagt ccgccgacag gagccgcggc cgcagcaggg tgccgtcgga
     2101 ccagtgcttg agcgccgggt gcgcatcgat cggcaggacg aggccgctgc ccgggtggtc
     2161 cccgacgttg ttgtcgttga ccgaggcgtc ccagtacgag atgagcagcc cgttctggta
     2221 gcggaagtgc tcgacccagt cgggccgggt gtcgaggaac ccgaagttgt acgccgtctt
     2281 gagcgaggcg tcgtagccgt cgtactgacg gttctcggcg acatacgcgt tgaagtagga
     2341 cgcctcgatc aggcccgagg tgcggatgaa cccgtcgaac acccagccct cggtgtcg
//
LOCUS       DATAIX010000004         4449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_149198, whole genome shotgun sequence.
ACCESSION   DATAIX010000004 DATAIX010000000
VERSION     DATAIX010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149198"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..352)
                     /locus_tag="VHM65_00040"
     CDS             complement(116..352)
                     /locus_tag="VHM65_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704123.1"
                     /translation="MSDLDVAIASPVLLARAKEIGVSTRARGTRAAPLWSHELDRLGL
                     RDLMRDLSGQAGRNVSFMVYASRESVLLRGDGIQ"
     gene            complement(349..705)
                     /locus_tag="VHM65_00045"
     CDS             complement(349..705)
                     /locus_tag="VHM65_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704124.1"
                     /translation="MVELEVAPAALVEAADAVDTILAARHATIRDLPTSVAHFGSAPA
                     GCRCRGLLRSLGRWAGTSARGQRGDGAALSPARALAGEARAHYRAYRTQVARREAAGA
                     SAAWTRWSPVSPSTWP"
     gene            814..1260
                     /locus_tag="VHM65_00050"
     CDS             814..1260
                     /locus_tag="VHM65_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704125.1"
                     /translation="MTFHDLPRGWDDGPVTDPSLVADVLDLFVSEQARATGALCLLLC
                     DEADRLLQPCTVGDLPHDLSVDERRLALRPFAQALGGSPRRGGILVAVGRDGADLVTA
                     SDIAWREAAESELREWDVRLLGVYVVTPNRCMPVPAPDGYAVAAAG"
     gene            1379..1741
                     /locus_tag="VHM65_00055"
     CDS             1379..1741
                     /locus_tag="VHM65_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704126.1"
                     /translation="MLAWLTTVVLLATVALAVVATIRLVRNDLIDDGVLLVAAVLEVG
                     LIAQLVVCATRMSQVSSAAERATFLAYAASLPFIPAGTAFLAIKEKSRWAMGVILVGA
                     FAIGVMTGRLQQIWNLNA"
     gene            1767..2168
                     /locus_tag="VHM65_00060"
     CDS             1767..2168
                     /locus_tag="VHM65_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704127.1"
                     /translation="MEPPPSATGAGPGRLLVGVYGVFALAATGRATVQIIEYFSRAPV
                     AYLLSALAAAIYLVATVALARGDRTSVRVATVAMSVELAGVLTVGLASYLMPSAFPDK
                     TVWSHFGSGYGYVPLVLPVVGLWWLRRHRVG"
     gene            complement(2172..3422)
                     /locus_tag="VHM65_00065"
     CDS             complement(2172..3422)
                     /locus_tag="VHM65_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX2704128.1"
                     /translation="MNHLVLAARPERRRLLVKRLRPVITGIVRLTAAVVIAFVLTSSL
                     LSSPAPVTGPLTALLVVQATLFATMATGARKVGAVLTGVLLAVGISTVVGLTWWSLGL
                     VVASALVVARLLPLKDQELEVAISAMLILAVSGSEVVAELRVVETLVGAAVGIALNLL
                     FPPRLHTPQATEAVEEVALKTADTLDRSAEKLAGAVRPSDLATWAHELRDIVPLVGEA
                     QTAIDDLSRSRRLNARAVSTPDASDPLRTGLSTIEHVLVAVRALYISMADGVPELRQP
                     ALESEPSVESEYASDLRAAFAVVLIDVSDTLRAFTTLVMAEAAFEGTQADAALEATLE
                     RLRETRAILTDLLLSHGDSDEAHWLTRGPVVAAIERILRELDIADWQRHRRDWAAEAE
                     RRAPVADALARLRDVPLPAWMLRG"
     gene            3730..>4449
                     /locus_tag="VHM65_00070"
     CDS             3730..>4449
                     /locus_tag="VHM65_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2704129.1"
                     /translation="MTSEEPSPAPPEDTTAPAGDPLGAPSGHLRPAPDAQVPYTLRVA
                     AGWAWRLVAVAAALWVVGQIAGALTFALVAVFVGGVITSLVKPLVDRLDRVLPRGVAV
                     ALSLVATIAGVIAIFSFIVAAFVNQIPELSAQVADGFASLRDWLRDSPLKADSTQIDQ
                     WLTSAQEWLKSNGANLAGGVLGGVGTVGELLTGLALAVFAAIFFLHDGGGIYAWIVET
                     TPKRVQPRFRTSGHLAWGTFSA"
BASE COUNT          606 a         1582 c         1583 g          678 t
ORIGIN      
        1 tccaatgtcc tcgttccgcc aggtcttgaa ccggatgccg agtctcaagg cgatcaggct
       61 catcacgctc tctcgagctt cggcgactcc cacccgtagg tgtcgtagcg cgtggtcact
      121 ggatcccatc tccgcggagc aggacgctct cccgggatgc gtacaccatg aagctcacgt
      181 tgcgaccggc ttgcccggag agatcccgca tgaggtcgcg caagccgaga cgatccagct
      241 catggctcca gagcggggcc gcgcgagtcc cccgcgcccg ggtgctgacc ccgatctcct
      301 tggcccgggc gaggagcact ggactcgcga tcgccacgtc caagtcactc acggccaggt
      361 cgaagggctc accggcgacc agcgggtcca tgcggccgat gcccctgcgg cttcgcgccg
      421 ggccacctgg gtccgatagg cgcggtagtg ggctcgggcc tcgcctgcaa gcgcccgagc
      481 gggcgaaagt gccgcgccat cgcctcgctg tccccgggca gatgtgcctg cccagcgtcc
      541 cagcgagcgc agaagtccgc ggcaacggca cccagccggg gcgctcccga agtgggccac
      601 cgacgtcggg aggtcccgga tcgtcgcgtg ccgcgcggcg aggatcgtgt cgaccgcatc
      661 cgccgcctcg acgagagccg ccggagccac ctcgagctcg accatggcat cgatgacacc
      721 agaacagccc agacggcctc ggaagttgtc cacaggtcac ggtcctgtgg ataacttccg
      781 gggccgaacg ccgtcgtaag gcaccctcgt cccatgacct ttcacgacct tccccggggc
      841 tgggacgacg gccccgtcac tgacccttcc ctcgtggccg acgtgctcga cctgttcgtt
      901 tccgagcagg ctcgcgccac cggcgccctc tgcctgctcc tgtgcgacga ggccgaccgg
      961 ctgctccagc cgtgcacggt cggcgacctg ccccatgacc tcagcgtcga cgagcggcgt
     1021 ctggcgctgc gcccctttgc gcaggccctc ggtggctcac cacgacgcgg cgggatcctc
     1081 gtcgccgtcg ggcgcgacgg cgcggacctt gtcactgcga gcgacatcgc ctggcgtgag
     1141 gcggccgagt ccgagctgcg ggagtgggac gtgcgcctgc tcggcgtcta cgtcgtgacg
     1201 cccaaccggt gtatgccggt ccccgcaccg gacgggtatg ccgtcgccgc tgccggctga
     1261 ggtcatcgcg ctgcggcagg cgtccggccg gccaggccga acaaccgccg cggaagttga
     1321 catgcggctc ggtcacgatg gcggccccag tcgctcggcc cgggttaggc tgctggtcgt
     1381 gcttgcgtgg ctcaccaccg tcgtcctgct cgccaccgtg gcgctcgccg tggtggccac
     1441 gatccggctc gtccggaacg acctcatcga tgatggggtc ctgctcgtcg cggcggtcct
     1501 cgaggtgggc ctcatcgccc agctcgtcgt ctgtgccacc aggatgagcc aggtgtccag
     1561 cgcggcggag cgagccacct tcctcgccta tgccgccagc ttgccgttca tccctgcggg
     1621 tacggccttc cttgccatca aggagaagag ccgctgggcc atgggcgtca tcctcgtcgg
     1681 ggccttcgcg atcggggtca tgaccgggcg gctccagcag atctggaacc tcaatgcctg
     1741 aggtgggtgg tcggcatacc gggtcggtgg aaccgccacc atcggcgacg ggtgcgggtc
     1801 ccgggcggct gctcgtgggc gtctacggcg tcttcgcgct cgcggcgacc gggcgggcga
     1861 ccgtgcagat catcgagtac ttcagcaggg caccggtcgc ctacctgctc tcggcgctgg
     1921 ccgcggcgat ctacctcgtt gcgaccgtcg cactggctcg cggggaccgg acctcggtgc
     1981 gagtggccac cgtcgcgatg tcggtcgagc tcgccggggt gctcacggtc gggctggcgt
     2041 cctacctcat gccgtcggcc tttccggaca agaccgtgtg gtcgcacttc gggtcgggct
     2101 atggctacgt cccgctcgtg ctgccggtgg tcggactctg gtggctgcgg cggcaccggg
     2161 tcggctagca cctatccgcg cagcatccag gccggcaggg gcacgtcccg cagccgggcg
     2221 agcgcgtcgg ccaccggggc gcgccgctcg gcctcggccg cccagtcgcg ccggtgccgc
     2281 tgccagtcgg cgatgtcgag ctcgcgcagg atccgctcga tcgcggcgac gacagggcca
     2341 cgggtgagcc agtgggcctc gtcggagtcc ccgtgagaga gcagcaggtc ggtgaggatg
     2401 gcgcgggtct cccgcagccg ctccagggtg gcctcgaggg ccgcgtctgc ctgggtcccc
     2461 tcgaacgcgg cctcggccat gacgagcgtc gtgaacgcgc gcagggtgtc gctgacgtcg
     2521 atgaggacga cggcgaacgc cgcccgcagg tcggacgcat actcgctctc caccgaaggt
     2581 tcggactcca gcgctggctg acgcagctct ggcactccgt cggccatgga gatgtagagc
     2641 gcacggacgg cgacgaggac atgctcgatc gtgctgagcc cggtgcgcag cgggtcgctg
     2701 gcgtcgggcg tggacaccgc ccgggcgttg aggcgccggg agcgggagag gtcgtcgatc
     2761 gccgtctggg cctcgccgac gagcgggacg atgtctcgca gctcgtgggc ccacgtcgcg
     2821 aggtccgacg ggcgcaccgc tccggccagc ttctcagcgc tgcggtcgag ggtgtcggca
     2881 gtcttcaggg ccacctcctc gacggcttcg gtcgcctgtg gggtgtgcaa ccgcgggggg
     2941 aagagcaggt tgagcgcgat gccgaccgcg gcaccgacaa gggtctcgac gacgcgcagc
     3001 tcggcgacca cctcggatcc gctcacggcg aggatgagca tggcgctgat ggcgacctcg
     3061 agctcctggt ccttgagcgg caggaggcgg gcgacgacca gcgctgacgc gacgacgagt
     3121 ccgaggctcc accacgtgag cccgaccacc gtcgagatgc cgacggcgag gaggacgccg
     3181 gtgaggaccg cgccaacctt gcgggcgccc gtcgccatcg tcgcgaacag ggtggcctgg
     3241 acgacgagca acgcggtgag tggtccggtg accggagccg gggaggacag gagcgacgag
     3301 gtgagcacga aggcgatgac cactgcagcc gtgaggcgca cgatcccggt gatgacggga
     3361 cggagccgct tgacgagcag ccgccgccgc tcggggcgcg ccgccaggac caggtggttc
     3421 acgccctccg accaggcccg acgcacgcga gtggcggctc ggcgagcgag ccgcaggggc
     3481 tggcctggcg tggtcatgtc cgccatcgtc ggcccaaccg gcgtctcgcc gctacctcgc
     3541 gcggccgaag gggcgctgcc tgggagctcc ctgggcgttc gcccgccgcg gctcaccctt
     3601 cgggaccggc tcccgacgac taacttgggt atcaccacga gcctcgacga caggagtaga
     3661 cccgatgacg cccgtcagaa cgacgtcggc ccgaccgggc cgaaccgacg gcaacgtcgg
     3721 tgcgccgaca tgaccagcga agagccctcg ccggcgccgc ccgaggacac caccgccccg
     3781 gccggtgacc cactcggggc gccgtcgggt cacctgcgtc ccgcgcctga cgcacaggtg
     3841 ccgtacaccc tgcgggtcgc tgccggctgg gcgtggcgtc tcgtcgccgt cgcggccgcg
     3901 ctctgggtcg tcgggcagat cgcgggtgcg ctgacctttg cgctcgtggc cgtgttcgtg
     3961 gggggtgtga tcacgtcctt ggtcaagccc ctggtcgacc gcctcgaccg cgtccttccc
     4021 cggggcgtcg cggtcgcgct atccctcgtc gcgaccatcg ccggcgtcat cgccatcttc
     4081 agcttcatcg tcgcggcctt cgtcaaccag atcccggagc tctccgcaca ggtggcggat
     4141 gggtttgcca gccttcgcga ctggctacgc gacagcccgc tcaaggcgga ctcgacccag
     4201 atcgaccagt ggctcacgtc cgcccaggaa tggctcaaga gcaacggtgc gaacctggcc
     4261 gggggtgtcc tcggcggggt cgggaccgtc ggggagctgc tcaccgggct ggcgctcgcg
     4321 gtgttcgcgg cgatcttctt cctccacgac ggcggcggca tctacgcctg gatcgtcgag
     4381 acgacaccga agcgtgtgca gccgaggttc cgcacgtccg gccacctggc ctgggggacg
     4441 ttctccgcc
//
LOCUS       DATAIX010000005         3427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_59816, whole genome shotgun sequence.
ACCESSION   DATAIX010000005 DATAIX010000000
VERSION     DATAIX010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59816"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2851
                     /gene="rpoB"
                     /locus_tag="VHM65_00075"
     CDS             <1..2851
                     /gene="rpoB"
                     /locus_tag="VHM65_00075"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX2704130.1"
                     /translation="VDRKRKQSVTVLLKALGWTNDRILEEFGDFESMRLTLEKDHTQG
                     QDDALLDIYRKLRPGEPPTKEAAQTLLDNLYFNHKRYDLAKVGRYKVNKKLGIEAPLS
                     ASTLSIEDVVAAIKYIVLLHAGETTVKGRRAGAEVDLRVEVDDIDHFGNRRLRSVGEL
                     IQNQVRTGLSRMERVVRERMTTQDVEAITPQTLINIRPVVASIKEFFGTSQLSQFMDQ
                     NNPLAGLTHKRRLSALGPGGLSRDRAGMEVRDVHPSHYGRMCPIETPEGPNIGLIGSL
                     ASYGRINAFGFVETPYRRVVKGMVTDEVHYLSADEEDEHVIAQANARLAVDGHFAEDR
                     VLVRTKGGEVEYIPGADVDYMDVSARQMVSAATALIPFLEHDDANRALMGSNMQRQAV
                     PLVKSEAPLVGTGMEYRAALDSGDVVRAEKAGVVSEVSADLVTVTNDDGTYQTYRIAK
                     FARSNQGTSYNQVVVVDEGDRVEAGAVIADGPATDGGEMALGRNLLVAFMPWEGHNYE
                     DAIILSQRLVQDDVLSSIHIEEHEVDARDTKLGPEEITRDIPNVSEEILADLDERGII
                     RIGAEVRDGDLLVGKVTPKGETELTPEERLLRAIFGEKAREVRDTSLKVPHGETGTVI
                     GVKVFDKDEGDDLAPGVNQLVRVYVANKRKITDGDKLAGRHGNKGVIAKILPIEDMPF
                     LEDGTAVDVVLNPLGVPGRMNVGQILELHLGWSAAQGWKIEGDPDWAKDISPEAREVV
                     PGTRVASPVFDGAGEDVITGLLDSTLETRDGVRLIDSSGKAKLYDGRSGEPFPEPVSV
                     GYMYILKLHHLVDDKIHARSTGPYSMITQQPLGGKAQFGGQRFGEMEVWALEAYGAAY
                     TLQELLTIKSDDVPGRVKVYEAIVKGENIPDSGIPESFKVLLKEMQSLCLNVEVLASD
                     GQAIEMKDSDDDVFRAAEELGIDLSRREPSSVEEV"
     gene            2953..>3427
                     /locus_tag="VHM65_00080"
     CDS             2953..>3427
                     /locus_tag="VHM65_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704131.1"
                     /translation="MLDVNFFDELRIGLATADDIRQWSHGEVKKPETINYRTLKPEKE
                     GLFCERIFGPTRDWECNCGKYKRVRFKGIICERCGVEVTRAKVRRERMGHIELAAPVT
                     HIWYFKGVPSRLGYLLDLAPKDLEKVIYFAAYMITSVDVDQRHRDLPSLEAQIQVE"
BASE COUNT          624 a         1191 c         1116 g          496 t
ORIGIN      
        1 cgtcgaccgc aagcgcaagc agtcggtcac cgtgctcctc aaggccctcg gctggaccaa
       61 cgaccgcatc ctggaggagt tcggcgactt cgagtcgatg cgcctcaccc tggagaagga
      121 ccacacccag ggccaggacg acgccctgct cgacatctac cgcaagctgc gtccgggcga
      181 gccgccgacc aaggaggccg cccagaccct gctcgacaac ctctacttca accacaagcg
      241 ctacgacctg gccaaggtcg gccgctacaa ggtcaacaag aagctcggca tcgaggcgcc
      301 actttccgcc tcgaccctgt cgatcgagga cgtcgtcgcg gcgatcaagt acatcgtgct
      361 gctccacgcg ggcgagacga cggtcaaggg ccgccgggcc ggtgcggagg tcgacctgcg
      421 cgtcgaggtc gacgacatcg accacttcgg caaccgccgc ctgcgcagcg tcggcgagct
      481 catccagaac caggtccgca cgggcctgtc ccggatggag cgcgtcgtgc gagagcggat
      541 gacgacccag gacgtcgagg cgatcacccc gcagaccctg atcaacatcc gccccgtggt
      601 ggcctcgatc aaggagttct tcgggacctc gcagctgtcc cagttcatgg accagaacaa
      661 cccgctcgcc gggctgaccc acaagcgccg cctgtcggcg ctcggcccgg gtggtctctc
      721 ccgggaccgc gccggcatgg aggtccgtga cgtgcacccg tcgcactacg gccgcatgtg
      781 cccgatcgag accccggaag ggccgaacat cggcctcatc ggctcgctcg cgtcgtatgg
      841 ccggatcaac gcgttcgggt tcgtcgagac gccctaccgc cgggtcgtca aggggatggt
      901 gaccgacgag gtccactacc tgtccgccga cgaggaggac gagcacgtca tcgcccaggc
      961 caacgcccgg ctcgcggtgg acggccactt cgccgaggac cgcgtcctcg tgcggaccaa
     1021 gggcggcgag gtcgagtaca tcccgggcgc tgacgtcgac tacatggacg tctcggcccg
     1081 ccagatggtg tccgcggcga ccgcgctcat cccgttcctc gagcacgacg acgccaaccg
     1141 ggcgctcatg ggctcgaaca tgcagcgcca ggcggtgccg ctcgtcaagt ccgaggcgcc
     1201 cctggtcggc accggcatgg agtaccgcgc cgcgctcgac tccggtgatg tcgtccgggc
     1261 cgagaaggcc ggcgtcgtct cggaggtgtc ggccgacctg gtcacggtga ccaacgacga
     1321 cgggacctac cagacctacc ggatagccaa gttcgcgcgc tccaaccagg gaacgtcata
     1381 caaccaggtc gtcgtggtcg acgagggcga ccgggtcgag gccggggcgg tcatcgccga
     1441 cggtcccgcg accgacggtg gcgagatggc gctcgggcgc aacctgctcg tcgcgttcat
     1501 gccgtgggag gggcacaact acgaggacgc gatcatcttg tcccagcgcc tcgtgcagga
     1561 cgacgtcctg tcctcgatcc acatcgagga gcatgaggtc gatgcccggg acaccaagct
     1621 cgggcccgag gagatcaccc gggacatccc caacgtctcc gaggagatcc tcgccgacct
     1681 cgacgagcgc gggatcatcc gcatcggcgc cgaggtccgt gacggcgacc tgctcgtcgg
     1741 caaggtcacg cccaagggtg agaccgagct gaccccggag gagcggctgc tgcgcgcgat
     1801 cttcggcgag aaggctcgcg aggtccgcga cacctccctc aaggtcccgc acggcgagac
     1861 cggcacggtc atcggcgtca aggtcttcga caaggacgag ggggacgacc tcgcccccgg
     1921 tgtcaaccag ctcgtccggg tctatgtcgc caacaagcgc aagatcaccg acggtgacaa
     1981 gctcgccggc cggcacggca acaagggcgt catcgccaag atcctgccca tcgaggacat
     2041 gccgttcctc gaggacggca cggccgtcga tgtcgtgctc aacccgctcg gggttcctgg
     2101 caggatgaac gtcggccaga tcctcgagct gcacctgggc tggtccgcgg cgcaaggctg
     2161 gaagatcgag ggcgaccccg actgggccaa ggacatctcg cccgaggccc gtgaggtcgt
     2221 ccccggcacc cgggtcgcct cgccggtctt cgacggtgcg ggcgaggacg tcatcaccgg
     2281 tctgctcgac tcgacgctgg agacccgtga cggggtccgg ctgatcgact cctcgggcaa
     2341 ggccaagctc tacgacggcc gctccggcga gccgttcccc gagccggtgt cggtgggcta
     2401 catgtacatc ctcaagctgc accacctcgt cgacgacaag atccacgcgc ggtcgacggg
     2461 cccgtactcc atgatcaccc agcagccgct cggcggtaag gcccagttcg gtggccagcg
     2521 cttcggtgag atggaggtct gggcgctcga ggcgtatggc gcggcctaca ccttgcagga
     2581 gctgctgacg atcaagtccg acgacgtccc cggccgggtc aaggtctacg aggccatcgt
     2641 caagggcgag aacatccccg actccggcat ccccgagtcg ttcaaggtgc tcctcaagga
     2701 gatgcagtcg ctgtgcctca acgtcgaggt cctcgcctcc gacggccagg ccatcgagat
     2761 gaaggactcc gatgacgacg tcttccgcgc agcggaggag ctcggcatcg acctgtcgcg
     2821 gcgcgagccg agcagcgtcg aagaggtctg acggcacggt gccgagaccg atcagggccg
     2881 agaccgatca cccgatcaga ccaacctgcc tgacctgggc gacttaccag ggttaacgag
     2941 agaaggaacc acgtgctcga cgtcaacttc ttcgacgagc tgcgcattgg cctcgccacc
     3001 gccgacgaca tccgtcagtg gagccatggt gaggtgaaga agccggagac catcaactac
     3061 cgcaccctca agcccgagaa ggagggcctg ttctgcgagc gcatcttcgg ccccacccgg
     3121 gactgggagt gcaactgcgg caagtacaag cgggtccgct tcaagggcat catctgcgag
     3181 cgctgcggcg tcgaggtcac ccgcgcgaag gtgcgccgtg agcggatggg ccacatcgag
     3241 ctcgccgcgc ccgtcacgca catctggtac ttcaagggcg tcccgtcgcg tctcggctac
     3301 ctcctcgacc tggcaccgaa agacctcgag aaggtcatct acttcgcggc ctacatgatc
     3361 acctcggtcg atgtcgacca gcgccaccgg gacctgccct cgctcgaggc acagatccag
     3421 gtggaga
//
LOCUS       DATAIX010000006        14056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_208614, whole genome shotgun sequence.
ACCESSION   DATAIX010000006 DATAIX010000000
VERSION     DATAIX010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 14056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14056
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208614"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1878)
                     /locus_tag="VHM65_00085"
     CDS             complement(175..1878)
                     /locus_tag="VHM65_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2704132.1"
                     /translation="MGHLEVNGVFFTLPDGRPLLRDVNLRVGDGAKVALIGPNGTGKT
                     TLTRIISADLVAPEGAVTRSGGLGVMRQFIGSVRDHSTVRDLLVSVAPAPIRAGAAEV
                     DRAELAIMERDSTADQLAYAQALVDWGDVGGYEAETLWDVCTISALGVPFERAQWRQV
                     STLSGGEQKRLVLEALLRGPDEVLVLDEPDNYLDVPAKRWLEDQLRTSDKTVLFISHD
                     RELLRRVASRVATLEPSAAGATAWVHPAGFASYQQARIDRNARLDELRRRWDEEHAKL
                     KALVLMYKQKAAYNDAMASRYQAAQTRLAKFVAAGPPEAVPLRQAVRMRLTGGRTAKR
                     AVICERLELTGLMAPFDVEIWYGERVAVLGSNGSGKSHFLRLLAAGGSDPDIEHQPVG
                     EPSADGVDTGAEPVRHTGTARLGSRVRPGWFAQTHEQPALRGRTLLEILHRGDAHRAG
                     KPREEASRALDRYGLARAAEQTFESLSGGQQARFQILMLELSGATLLLLDEPTDNLDL
                     DSAESLEEALDAFDGTVVAVTHDRWFARSFDRFLVFGSDGEVYEAAEPVWDETRVRRS
                     R"
     gene            complement(1987..7290)
                     /gene="pulA"
                     /locus_tag="VHM65_00090"
     CDS             complement(1987..7290)
                     /gene="pulA"
                     /locus_tag="VHM65_00090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA];
                     GO_process: GO:0005983 - starch catabolic process
                     [Evidence IEA];
                     GO_process: GO:0051678 - pullulan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase-type alpha-1,6-glucosidase"
                     /protein_id="HEX2704133.1"
                     /translation="MADRFANGSSANDAGGLTGDRLVTGLDPTDKGFFHGGDLTGLTQ
                     RLDYIKDLGTTAIWLTPTFKNRPVQGAAPDISAGYHGYWITDFTQIDPHLGTNAEMRT
                     LISAAHAKGMKVFFDIITNHTADVIAYDGGGTSYVSKETKPYQDAAGTVFDDKTYAGG
                     TTFPAIAAASSFPYVPVFPTTADETVKVPSWLNDTTMYHNRGNSTFAGESSEYGDFSG
                     LDDLWTERPEVVSGMTDVYKAWVDLGIDGFRIDTVKHVNLEFWQRFSPAILDHATASG
                     SAKFFMFGEVFDGSPGYVSTFTTKGALQATLDFPFQASALAFAQGKATSGVRDMFAQD
                     DYYTDTDSNAYESPTFLGNHDMGRVGMMLSKAGYTGTELLTRTRLATSLMFLTRGQPV
                     VYYGDEQGFAGLGGDKDAREDMFGTQVEQYAAEPLVGGGTMGTSAHFDRDAPLYRSTK
                     ALAALRAAHPALADGAQIPRYSSGAAGIFAVSRIDRARRTEYLVVTNNATEPKTVTLA
                     TYSPSTSFAPIYGAAGSQRSGADGRVTLTVPPLSARVYQAQQPMPARSSALPVFLTSP
                     SAGGVVGGRAEIGAAIPANAFAQVTFLTRPVGTSAWTTLGTDDHAPYRVFHDVTSVAK
                     GTLMEYRALAKDVSGNVSARSSYGIVGDPAPGGGGGGGGGPVVQPDAASVPGSHNGEM
                     GCTDDWQPDCDQAQLTLDPNDQIWKGTYGSVPAGDYDYKVAIDKSWDENYGVGAIRGG
                     SNIAYTAPGSDIHFYYDHGTHWVTSDAQSPIITAPGTFQSELGCPADFSPDCMRPWLQ
                     DPDGDGTWTWTSDQIPAGDYELKVAHGLGWGENYGVGGVRDGANIAFSVPSDGLLVTF
                     SYVLATHLVSVSTSRAGASPDLTKAKAYLVGRDLVAWPATAVPAGVSPEVLRWRLHWS
                     GNGGLGLDEEAVTTGSVADLTRDAAGLPAALVQAHPELTGYIALRLAPQTARQIRSIL
                     TGQVAVAMYDTTSRLLDATGVQIPIGLDAMYGASASQRTYGVSWSRDVPTYRVWAPTA
                     KRVALLVWPRGTDPSGPVSAAKRLAMSRAADGSWSRTGVPAQRGAAYLYEVTVYAPST
                     RQVETNLVTDPSSVALTLDSTRSVVVDLRDRAWQPDQWRTAASPRLGSPVDSTIYELH
                     VRDFSVADTSVPAEHRGSYLAFADDGVGTRHLRALAEAGLNTVHLLPTFDIASIEEDP
                     AAQTSPDCDLAAGAPDGATQQACVTAQSATDAFNWGYDPWHWSVPEGSYASTAAAAEG
                     GARVAEFRTMVGALHGLGLRVVLDEVFNHTPASGQDEKSVLDRIVPGYYHRLGATGAV
                     ETSTCCQNVATEHAMAQKAMVDSVVTWAKGYKVDGFRFDLMGHHSKANLLAIRKALDG
                     LTRAKDGVDGRAVYLYGEGWNFGEVADNKLFVQATQGQLGGTGIGTFSDRLRDAVRGG
                     GPFDDDPRKQGFGSGVSTDFNGAPANGDASAQAVTLAHDTDLVQLGLAGNLRSFTFTS
                     QASGQSVRGDQVDYNGSPAGYADQPDEVITYVDAHDNETLFDALTFKLPVATSMADRV
                     RMNTVSLATVALAQTPAFWHAGADVLRSKSLDRNSYDSGDWFNRLDFTGADNGFGHGL
                     PPEGDNAGKWDYMRPLLANAALTPTTEDVARASAQAQDLLRLRFSTPLFRLGSADLIN
                     AKVSFPASGVADSHPGVIVLRIDDLAGPDVDPALTGLVVVFNASPAPVTQTVPGLSGA
                     TLELSPVQAGGSDEVVKGATWDAGAGSASVPARTVAVFVQR"
     gene            complement(7314..7799)
                     /locus_tag="VHM65_00095"
     CDS             complement(7314..7799)
                     /locus_tag="VHM65_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704134.1"
                     /translation="MASSRRARTVRTRLVTGVSSAAVVLAAAVMPLAPGAAVAVDGRT
                     ATLVGSLQSELGCGADWDPGCEATTLTAGEGTSYAKTFDVPAGSYELKLVINHAWDEN
                     YGAGGAMGGANIPVVLGGPASLTFTYDDTSHRVAITTTSVAGAVTGRTGGSPGTACAP
                     D"
     gene            8058..8699
                     /locus_tag="VHM65_00100"
     CDS             8058..8699
                     /locus_tag="VHM65_00100"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2704135.1"
                     /translation="MRPEEIASALRRAVHERTLPPGEYLNQDELARRFGVSRIPLREA
                     LRTLVGEGLIIMKPGLGAVIAELDVGEVKELYQLRLQLEPPLAPDIIDNVRRRDVSDL
                     RALSESLAHLSAEGSPDWAAANYSFQRKLYEISGKRHAVRLVVQTLNLVEPYARLHLH
                     RPQARAEADQQRAQIIAALEQGDEPTLVAVITRGIERTRDALVDMMPPPEPVG"
     gene            8845..10056
                     /locus_tag="VHM65_00105"
     CDS             8845..10056
                     /locus_tag="VHM65_00105"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX2704136.1"
                     /translation="MSATIAACALATAACGSKGEAGPAAGSGSTTLTLGASVSLTGKL
                     AREGTLTQEGYKLCVDKVNAKGGVKVGDQTLKLDIKYQDDTSKPDTAAQLVDQFNDQG
                     IKLLLGSYGSANTEAQAAVIERNAQVMVDSSGADDKIFAKGYKRTFAVLSPATEYAAS
                     IVKAINDLASPKPKSIVFLSADDGFSKTATEGGIKVAKELGMTVFDTQFFPNGATDVS
                     SALNKAKGSRPDVILGSVHLAEGVAIVKQAKELGITPMAFGETVAPPTPDFVSTLGKQ
                     AEGVLGSSQWTKAATGTDDYFGTAKTYDADIQAAFGHAAGYHNAEASGACLALVLAAE
                     KAGSTDPTKVRDALAALDVDSFFGRIKFDATGKNVTKPMAVIQIQSGKAVTVWPKEMT
                     EAPLVWPGTGS"
     gene            10053..10937
                     /locus_tag="VHM65_00110"
     CDS             10053..10937
                     /locus_tag="VHM65_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX2704137.1"
                     /translation="MSGLLQAVVYGLLQGGLLALVAVGFSLVWGVMNVVNLSHGAFVV
                     LGAYVGLQLNQHLGLDPFLAMFVSAVALFVLGYAVQRFLVNLVVNGPIFITLLLTFGL
                     ELLLVQGMNLAFTANYQSIQTSYAGTSFAVGEIQVPLGRLLAFLLAIAVTLALVSVMT
                     KTRVGMSIMATGMDRGAARLMGIRARHVYALTFGIAAGLAGMAGTMVGTVGTFNPASA
                     GGFTLLSFVIAVLGGLGNMYGALAGGILIGLVQALGGYYLPGTWVNALAFLVLILVLM
                     FRPSGLVGKAFYSARLEV"
     gene            10940..11992
                     /locus_tag="VHM65_00115"
     CDS             10940..11992
                     /locus_tag="VHM65_00115"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX2704138.1"
                     /translation="MALDDTMTAAGGAGPAPSRRSTRERVRVARPWALGVGLVLGALL
                     LQTQLYSGQARTVTAIFMFVALAQSWNLIGGFTGYASFGQVVFFGIGGYTTAVAMTNW
                     HWTFWTSLVLSGVVAALYGAVVGMPLLRLRGHYFAIATLGMAEGTREIVTNLPDLTGG
                     GGGITLPTVGAGATTAYLGNDGFYLVFLALAALGLLVTGVVSRSKFGFALRAIHQDED
                     AAAAVGINTTKTKILTFALSGFLAGLVGATYAFQQVTIYPERLFDVEITVLMVVMVVI
                     GGSGTVIGPVIGAVGLQFLSEWLRQNYTSIHTLLLGGIIIVAVILLPQGIANYLSEAR
                     RTGEYSLLANVRRYRL"
     gene            11989..12750
                     /locus_tag="VHM65_00120"
     CDS             11989..12750
                     /locus_tag="VHM65_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2704139.1"
                     /translation="MSGAPVLETRGLGRRFGGLKAVDDLSISVAEGLTLGVIGPNGSG
                     KSTFINLVTGHLKPTTGSVLIDGTDLTGAKPWVIAAARVARTFQIVKPFRGMTVRQNV
                     AVGIMYGPEGTARMAPALRQADDVLAEVGLAGSGDRAPGELTVADARRLEFAKALALR
                     PRLLLLDEVMAGLRPAEIEPSLALIQQLKQGGMTIVVVEHVMKAILAVSDEVMVLHQG
                     AVLMRGSPREVLSDPRVIEAYLGQKYAKRQAGEHA"
     gene            12743..13435
                     /locus_tag="VHM65_00125"
     CDS             12743..13435
                     /locus_tag="VHM65_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2704140.1"
                     /translation="MLEVSGLQAGYGRVQVLWDVEIRVGSKEIVSLVGPNGAGKTTLL
                     RALSGMVPATAGTMTFRGKDLRGLSIEQIVDLGISHVPEGRRLFPGLTVRDNLRLGGW
                     RNRNHNLDRVIELFPKLGERINQVCGTMSGGEQQMCAIGRGLMGEPELMMIDELSLGL
                     APVVVEEIIARLPDISAAGTSVLLVEQDVDAALSAAERAYVLETGHIVMTGKAGELLV
                     DPRIRASYLGIA"
     gene            complement(13679..>14056)
                     /locus_tag="VHM65_00130"
     CDS             complement(13679..>14056)
                     /locus_tag="VHM65_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704141.1"
                     /translation="ALNEASAGLLGLHDFAAFCRRREGATTTRTLLGFAWARDGDGVL
                     VASVRADAFCHSMVRSLVGAVLPAGQGRVETGWPAEVLSAGRRDPRVTVMPAHGLCLE
                     EVAYPPVERLAIRARQARARRED"
BASE COUNT         2067 a         4919 c         4866 g         2204 t
ORIGIN      
        1 ccgtcagacc tgtggcgcga gccggcactc gttccttggc caagtcatga gcggcgtggg
       61 cagccaggga gtcaatccgc ccgagatagc ggtcaggact tccgacgacc ccgctgatcg
      121 agccggccac gaggttgtgg ccggaatgct cggccgtcgc cgggcgcggg tggctcagcg
      181 ggagcgccgg acccgcgtct cgtcccacac gggttcggcc gcctcgtaga cctccccgtc
      241 ggagccgaac acgaggaacc ggtcgaacga acgggcgaac caccggtcgt gggtgaccgc
      301 gacgaccgtc ccgtcgaagg cgtccagcgc ctcctcgagc gactcggccg agtcgaggtc
      361 gaggttgtcc gtgggctcgt cgaggaggag cagcgtggcg ccgctcagct cgagcatgag
      421 gatctggaag cgcgcctgct gcccaccgga gagggactcg aaggtctgct cggccgcgcg
      481 agcgagcccg tagcggtcga gggcccgcga ggcctcctca cggggtttgc cggcccggtg
      541 agcgtcgcca cgatgaagga tctcgagcag cgtgcgtccc cgcagcgcgg gctgctcgtg
      601 ggtctgcgcg aaccatcccg gccgcacccg cgagccgagc cgcgccgtac ccgtgtgacg
      661 caccggctca gcaccggtgt cgacgccgtc ggccgacggc tcccccaccg gctggtgctc
      721 gatatcgggg tcggagccgc ccgcggcgag cagccgcagg aagtgggact tgcccgagcc
      781 gttggacccg aggaccgcga cgcgctcgcc gtaccagatc tcgacgtcga acggcgccat
      841 gagaccggtc agctcgagcc gctcacagat gaccgcccgc ttggcggtcc gccccccggt
      901 gaggcgcatc cgcacggcct ggcgcagcgg gacggcctcg ggcggcccgg ccgcgacgaa
      961 cttcgccagc cgggtctggg ccgcctggta gcggcttgcc atggcgtcgt tgtaggccgc
     1021 cttctgcttg tacatcagca cgagggcctt gagcttggcg tgctcttcgt cccagcgccg
     1081 gcgcagctcg tcgagccgcg cgttccggtc gatccgggcc tgctggtatg acgcgaagcc
     1141 ggcggggtga acccaggccg tcgccccggc cgcgctcggc tccaaggtgg ccacccggct
     1201 ggcgacccgg cggagcagct cgcggtcgtg actgatgaac aggaccgtct tgtccgaggt
     1261 gcgcagctgg tcctcgagcc agcgcttggc cggcacgtcg aggtagttgt cgggctcgtc
     1321 gaggaccagg acctcgtcgg ggccgcgcag cagggcttcg agaacgaggc gcttctgctc
     1381 gccgccggac agggtgctga cctgacgcca ctgggcccgt tcgaagggca ccccgagcgc
     1441 ggagatcgtg cagacgtccc agagcgtctc ggcctcgtag ccgccgacgt cgccccagtc
     1501 gacgagcgcc tgcgcgtagg cgagctggtc cgccgtgctg tcgcgctcca tgatcgcgag
     1561 ctcggcgcgg tcgacctcgg ctgcaccagc gcggatgggc gcaggcgcga cggacaccag
     1621 caggtcgcgc acggtggagt ggtcccgcac cgagccgatg aactggcgca tgaccccgag
     1681 cccgccactg cgggtgaccg ctccctcagg ggcgacgagg tcggccgaga tgatccgggt
     1741 caacgtcgtc ttgcccgtgc cgttggggcc gatcagcgcg accttggcgc cgtcaccgac
     1801 ccgcaggttg acatcgcgca acagcggccg cccgtcaggc agggtgaaga acacgccgtt
     1861 gacctcgaga tgacccacaa tccccaagcc tgacgtatgc cgggcacccg cgtcacctct
     1921 gtttgcctcg acggcctcgg ccgtggcggg tcggggtcgc accgtctccg ccacgggtcg
     1981 gcctggtcag cgctggacga acaccgccac ggttcgcgcg ggaaccgacg ccgacccggc
     2041 gccggcatcc caggtcgcgc ccttgacgac ctcgtcgctc ccgcccgcct gcaccggcga
     2101 gagctccagc gtggcgcccg acaggccggg caccgtttgg gtgaccgggg ccggcgacgc
     2161 attgaacacg acgacgagcc ccgtgagggc aggatccacg tccggaccgg cgaggtcgtc
     2221 gatgcgcagg acgatgaccc cgggatgcga gtcggccacg cccgacgccg ggaaggagac
     2281 cttcgcgttg atcaggtcgg cggacccgag ccggaacagc ggcgtcgaga agcgcagccg
     2341 caggaggtcc tgcgcctggg cgctcgcgcg cgcgacgtcc tctgtcgtcg gcgtgagggc
     2401 ggcattggcg agcaacgggc gcatgtagtc ccacttgccc gcattgtcgc cctcgggcgg
     2461 caggccgtga ccgaacccgt tgtcggcccc ggtgaagtcg agccggttga accagtcacc
     2521 gctgtcgtag gagttacggt ccaggctctt gctgcgcagc acgtcggctc ccgcatgcca
     2581 gaacgcaggg gtctgggcca gagcgaccgt cgcgagcgag acggtgttca tccggacccg
     2641 gtcggccatc gaggtcgcca cgggcagctt gaaggtcagc gcgtcgaaga gggtctcgtt
     2701 gtcgtgggcg tcgacatagg tgatgacctc gtccggctgg tcggcatacc cggccgggga
     2761 gccgttgtag tcgacctggt caccgcggac gctctgcccc gacgcctggc tggtgaaggt
     2821 gaaggaccgc aggttgccgg cgagccccag ctggaccagg tcagtgtcgt gggcgagcgt
     2881 gacggcctgg gccgacgcgt cgccgttggc cggcgcaccg ttgaagtcgg tcgacacccc
     2941 cgagccgaag ccctgcttgc gcgggtcgtc gtcgaacggc ccaccgccac ggaccgcatc
     3001 gcggagccgg tcggagaacg tcccgatccc ggtgccgccg agctgaccct gggtggcctg
     3061 gacgaacagc ttgttgtcgg ccacctcgcc gaagttccag ccctccccgt agaggtagac
     3121 cgcccggccg tcgaccccgt ccttggcacg ggtgaggcca tcgagggcct tccggatcgc
     3181 gaggaggttg gccttggagt gatggcccat gaggtcgaac cggaacccgt cgaccttgta
     3241 gcccttggcc caggtgacca cggagtcgac catcgccttc tgtgccatgg cgtgctcggt
     3301 cgcgacgttc tggcagcacg tcgaggtctc cacggcacct gtcgcaccga gccggtggta
     3361 gtagccgggc acgatccggt caagcaccga cttctcgtcc tgacctgacg ccggagtgtg
     3421 gttgaacacc tcgtcgagca cgacccgcag acccaggccg tgcagggcgc cgaccatcgt
     3481 gcggaactcg gcgacccggg ccccgccctc ggcggccgcg gcggtcgagg cgtaggaccc
     3541 ctccgggacg ctccagtgcc aggggtcata gccccagttg aaggcgtcgg tcgccgactg
     3601 ggccgtgacg catgcctgct gggtcgcacc gtccggcgcc ccggccgcga ggtcgcagtc
     3661 cggcgaggtc tgcgccgcgg ggtcctcctc gatcgaggcg atgtcgaagg tgggcagcag
     3721 gtggacggtg ttgagcccgg cctcggccag cgcacgcagg tgtcgcgtgc cgacgccgtc
     3781 gtcggcgaac gcgaggtagg acccgcggtg ctcggccggc accgaggtgt cggcgaccga
     3841 gaagtcccgg acgtgcagct catagatcgt cgagtcgacg ggtgagccaa gccgcgggga
     3901 cgcggcggtc cgccactggt cgggctgcca ggcccggtcg cgcaggtcga cgacgaccga
     3961 gcgggtcgag tccagcgtca gcgcaaccga gctcgggtcg gtgacgaggt tggtctcgac
     4021 ctggcgtgtc gagggggcgt agaccgtcac ctcgtagagg taggccgccc cccgctgggc
     4081 gggcacgccg gtcctgctcc acgacccgtc cgcggcccgg ctcatggcga gccgcttcgc
     4141 tgcactcacc ggcccgctgg ggtccgttcc gcgcggccac acgagcaacg cgacccgctt
     4201 cgccgtcggt gcccacaccc ggtaggtcgg cacgtccctg gaccagctga ccccataggt
     4261 ccgctgggag gccgaggcgc catacatcgc atcgagcccg atggggatct gcactcccgt
     4321 cgcgtcgagg agccgcgatg tcgtgtcgta catcgcgacg gcaacctggc cggtcaggat
     4381 cgagcggatc tggcgcgccg tctgcggggc aagacggagc gcgatgtagc ccgtgagctc
     4441 cgggtgggcc tggacaagcg ccgccgggag ccccgcggcg tcgcgggtca ggtcggcgac
     4501 cgaccctgtg gtcacggctt cctcgtcgag gccgagtccg ccgttgcccg accagtgcag
     4561 tcgccaccgg agcacctcag gactgacgcc cgccggcacc gcagtggccg gccaggcgac
     4621 gaggtcccgg ccgacgaggt acgccttggc cttggtgagg tccgggctgg ccccggcccg
     4681 cgaggtgctc acgctgacca ggtgggtcgc gaggacgtag ctgaaggtga cgaggagacc
     4741 gtcgctcggc acgctgaacg cgatgttggc tccgtcacgg actcctccaa cgccgtagtt
     4801 ctcgccccag cccaggccgt gggcgacctt gagctcgtag tctcccgcgg ggatctggtc
     4861 cgaggtccag gtccaggtcc cgtcgccgtc ggggtcctgc agccacggcc gcatgcagtc
     4921 cgggctaaag tccgcggggc agcccagctc gctctggaag gtccccggcg ccgtgatgat
     4981 cgggctctgc gcgtcggagg tgacccagtg cgtcccgtgg tcgtagtaga agtggatgtc
     5041 gctccccggc gctgtgtacg cgatgttgga accaccgcgg atcgccccga cgccgtagtt
     5101 ctcgtcccag gacttgtcga tggcgacctt gtagtcgtag tcgcctgccg ggaccgagcc
     5161 ataggtgccc ttccagatct ggtcgttcgg gtccaaggtc agctgggcct ggtcgcagtc
     5221 gggctgccag tcgtcggtgc agcccatctc gccgttgtgg ctgcccggaa cgctggcggc
     5281 atcgggctgc acgaccgggc caccgccgcc accgccgccc ccacccgggg ccgggtcacc
     5341 gacgatgccg taggacgagc gcgccgacac gttgccgctg acgtccttgg ccagggcccg
     5401 gtactccatc agcgtgccct tggcgacgct cgtcacgtcg tggaacaccc ggtatggcgc
     5461 gtggtcgtcc gtgccgaggg tggtccaggc gctggtcccc accgggcggg tgaggaaggt
     5521 gacctgggcg aaggcgttgg ccgggatggc tgcaccgatc tcggcccggc cgccgacgac
     5581 ccccccggcc gacggcgagg tgaggaagac cggcagggca ctcgagcgtg ccggcatcgg
     5641 ctgctgcgcc tggtagacgc gggcggacag cggcggcacg gtcagggtca cccgcccgtc
     5701 ggccccgctg cgttggctgc cggccgcgcc gtagatcggg gcgaacgagg tgctcggaga
     5761 gtacgtcgca agcgtgacgg tcttcggctc ggtcgcattg ttggtcacga cgagatactc
     5821 ggtgcggcgg gcgcggtcga tccggctcac cgcgaagatg ccggccgccc cggacgagta
     5881 ccgcgggatc tgggctccgt cggcgagcgc ggggtgggca gcgcgcagtg ccgcgagggc
     5941 cttggtgctg cggtagagcg gggcgtcgcg gtcgaagtgg gcgctcgtgc ccatggtgcc
     6001 gccgccgacg agtggctccg cggcatactg ctcgacctgt gtcccgaaca tgtcctcgcg
     6061 cgcgtccttg tcgccaccca ggccggcgaa gccctgctcg tcgccgtagt agacgaccgg
     6121 ctggccgcgg gtgaggaaca tgagcgaggt ggccagcctg gtgcgggtga gcagctcggt
     6181 accggtgtac ccggccttgc tcagcatcat cccgacgcgc cccatgtcgt ggttgccgag
     6241 gaacgtcggc gactcgtagg cgttggagtc ggtgtccgtg tagtagtcgt cctgggcgaa
     6301 catgtcccgg accccgctcg tggccttgcc ttgggcgaac gcgagcgcgc tggcctggaa
     6361 ggggaagtcg agcgtcgcct ggagcgcgcc cttcgtcgtg aaggtgctca cgtagcccgg
     6421 gctgccgtcg aagacctcac cgaacatgaa gaacttcgcg ctccccgagg ccgtcgcatg
     6481 gtcgaggatc gccggtgaga accgctgcca gaactcgagg ttgacgtgct tgaccgtgtc
     6541 gatccggaaa ccgtcgatgc ccaggtcgac ccaggccttg tagacgtcgg tcatccccga
     6601 cacgacctcc ggccgctcgg tccagaggtc gtcgaggccg gagaagtcgc cgtactcgct
     6661 cgactcgccg gcaaacgtcg agttgccccg gttgtggtac atggtcgtgt cgttgagcca
     6721 gcttgggacc ttgacggtct catccgccgt ggtcgggaag accgggacgt aagggaacga
     6781 gctcgctgcg gcgatggcgg ggaaggtggt cccgccggcg tacgtcttgt cgtcgaagac
     6841 ggtcccggcc gcgtcctggt agggcttggt ctccttggag acgtaggacg tccccccgcc
     6901 gtcgtaggcg atgacgtcgg ccgtgtggtt ggtgatgatg tcgaagaaga ccttcatccc
     6961 cttggcgtgc gccgccgaga tgagcgtcct catctcggcg ttggtgccga ggtgagggtc
     7021 gatctgggtg aagtcggtga tccagtagcc gtggtagccg gcgctgatat ccggcgcggc
     7081 gccctggacg ggccggttct tgaacgtcgg tgtgagccag attgccgtcg tccccaggtc
     7141 cttgatgtag tcgagccgct gggtcaggcc ggtgaggtca ccgccgtgga agaagccctt
     7201 gtcggtggga tcgaggcccg tcacaagccg gtcgccggtc agcccgcccg cgtcgttggc
     7261 gctcgacccg ttggcgaacc ggtcggccat gacgaagtag aaccgctcct tggtcagtcc
     7321 ggcgcgcagg ctgtccccgg cgagcccccg gtcctgcccg tcaccgcccc ggccaccgag
     7381 gttgtcgtga tcgcgacccg gtgactggtg tcgtcatacg tgaaggtgag cgaggccggg
     7441 ccgccgagga cgacggggat gttcgcgcca cccatggcgc cgcccgcccc gtagttctcg
     7501 tcccacgcgt ggttgatgac cagcttgagc tcgtagctgc ccgccggcac gtcgaacgtc
     7561 ttggcgtatg acgtcccctc cccggccgtc agggtcgtcg cctcgcagcc cgggtcccag
     7621 tcggcgccac acccgagctc ggactgcagc gaccccacca gtgtcgccgt ccgcccgtcg
     7681 accgcgacgg ctgcgcccgg tgccaacggc atcaccgcgg cagcgaggac gactgccgcc
     7741 gagctgactc ctgtcacgag ccgggtccgg acggtgcgag cgcgccgtga cgaggccact
     7801 tcggctcctt tgctgggcgg atctggctga cgagtcgaca aatagggttc gccaccgaca
     7861 ggccgccaga cgcagatgca agaatgtgca cgctcctgga gcaccgcggg caccggctct
     7921 tcgggcgttg taccttcttg gcctgacagg gcccgctggt cggccggcac tgcgggctga
     7981 tcggcacgac agttgaggtg tcatggtggg gaacgcaagc aacgctcggt gggcaccgcc
     8041 cggtcacggg gggcgccgtg cggcctgagg agatcgcgag cgcgttgcgc cgggcggtgc
     8101 atgagcggac gctgcccccc ggggagtacc tcaaccagga cgagctcgcc cgccggttcg
     8161 gcgtcagccg gatcccgctg cgagaggcgc ttcgcaccct cgtcggcgaa gggctgatca
     8221 tcatgaagcc tggcctcggg gccgtcatcg ccgagctcga tgtgggcgag gtcaaggagc
     8281 tctaccagct gaggctgcag cttgagcccc cgctcgcgcc ggacatcatc gacaacgtcc
     8341 gacgacgtga cgtcagtgac ctgcgggccc tgtcggagtc gttggcccac ctctcggccg
     8401 agggatcgcc ggactgggcg gccgccaact actccttcca gcgcaagctg tacgagatct
     8461 ccggcaagcg ccacgccgtc cggcttgtcg tgcagacgct caacctcgtc gagccgtatg
     8521 cccgcctgca cctgcaccgt ccgcaggctc gtgcggaagc cgaccagcag cgtgcgcaga
     8581 tcatcgctgc cctcgagcag ggcgacgaac cgactctcgt tgcggtcatc acccgtggca
     8641 tcgaacgcac ccgtgacgcg ctggtcgaca tgatgccgcc gccggagccg gtcggctgac
     8701 ccgaaccctt tggcggccgt ctcgcctcac ctggcccgtg acgggtgccc aaactttctg
     8761 ttgccgtcga caccggctct gcatacagtg aataatccgc acctgcgacg gaaggcaact
     8821 gccgtgacgg ctcaacgcat tatcgtgtca gcgaccatcg cagcgtgcgc gctggcgacc
     8881 gctgcctgcg gctccaaggg cgaggccggg ccggccgccg gctccgggtc caccaccctc
     8941 accctcggcg cctccgtctc gctgacgggc aagctcgccc gcgagggcac cctcacccag
     9001 gagggctaca agctctgtgt cgacaaggtc aatgccaagg gcggggtcaa ggtcggcgac
     9061 cagacgctca agctcgatat caagtaccag gacgacacgt ccaaaccgga caccgccgcc
     9121 cagctcgtcg accagttcaa cgaccagggc atcaaactgc tgctcggctc ctatggatca
     9181 gccaacaccg aggcgcaggc cgccgtcatc gagcgcaacg cccaggtcat ggtcgactcc
     9241 tccggagcgg acgacaagat cttcgccaag ggctacaagc gcaccttcgc cgtcctgtca
     9301 ccggccaccg agtatgccgc ctccatcgtc aaggcgatca acgacctggc ctcgcccaag
     9361 ccgaagtcga tcgtcttcct ctcggccgac gacggcttct ccaagaccgc aaccgagggg
     9421 gggatcaagg tcgccaagga gctcggcatg acggtcttcg acacgcagtt cttccccaac
     9481 ggcgcgaccg acgtgagctc ggccctcaac aaggccaagg ggtcgcgtcc cgacgtgatc
     9541 ctcggctcgg ttcacctggc cgaaggagtc gccatcgtca agcaggccaa ggagctcggc
     9601 atcacgccaa tggcttttgg tgagacggtc gccccgccca cccccgactt cgtgagcacc
     9661 ctgggcaagc aggccgaggg agtgctcggg tcgagccagt ggaccaaggc agccacgggc
     9721 accgacgact acttcggcac ggccaagaca tacgacgcgg acattcaagc cgccttcgga
     9781 catgccgccg gctaccacaa cgcggaggcc agcggcgcct gcctcgcgct cgtcctggcc
     9841 gccgagaagg caggctcgac cgaccccacc aaggtccggg atgccttggc cgcactcgat
     9901 gtcgactcgt tcttcggccg gatcaagttc gacgctacgg gcaagaacgt caccaagccg
     9961 atggccgtca tccagattca gagcggcaag gccgtgacgg tctggccgaa ggagatgacc
    10021 gaagccccgc tcgtctggcc tggcacgggg tcgtgagcgg actgctgcag gccgtcgtct
    10081 acggcctgct gcaaggtggc ctgctcgccc tcgtcgccgt cggtttctcc cttgtctggg
    10141 gggtgatgaa cgtcgtcaac ctctcccatg gcgcgttcgt cgtgctcggg gcctacgttg
    10201 gtctccagct caaccagcac ctggggctcg accccttcct cgcgatgttc gtctcggcgg
    10261 ttgcactctt cgtgctcggg tacgccgtcc agcgcttcct cgtcaacctc gtcgtcaacg
    10321 gcccgatctt catcaccttg ctgttgacct tcggcctgga gctcctcctg gtccagggga
    10381 tgaacctcgc cttcaccgcc aactaccaga gcatccagac ctcgtatgcc ggcacgagct
    10441 tcgccgtcgg cgagatccag gttccgctcg ggcggctgct cgccttcctg ctggccattg
    10501 ccgtcaccct ggccctggtc agcgtcatga ccaagacccg ggtggggatg tcgatcatgg
    10561 cgaccggcat ggaccgcggc gccgcccgac tcatgggcat ccgcgccagg cacgtctacg
    10621 ccctcacctt cggcatcgcc gccggcctcg cgggcatggc gggcacgatg gtcggcacgg
    10681 tcggcacgtt caacccggcg tcagccggag gcttcaccct cctgtcgttc gtcatcgcgg
    10741 tcctcggtgg cctcggcaac atgtatggcg cgctcgccgg cgggatactc attggacttg
    10801 tccaggcgct gggcggctac tacctgccgg ggacctgggt caacgccctg gcgttcctcg
    10861 tcctcattct cgtgctgatg ttccgtccct ccgggctggt gggcaaggcc ttctactcgg
    10921 ctcgcctgga ggtgtgatca tggccctcga cgacaccatg accgccgcgg gcggcgccgg
    10981 accagcgccg tcgaggaggt cgacccgcga gcgagtccgg gtggcccggc cgtgggcgct
    11041 cggggtcggg ctcgtccttg gggcgctcct gctgcagacc cagctctact ccggccaggc
    11101 ccgcacggtg accgcgatct tcatgttcgt ggcgctggcc cagtcgtgga acctcatcgg
    11161 tggcttcacc ggctatgcct ccttcgggca ggtcgtcttc ttcggcatcg gcgggtacac
    11221 gaccgcggtt gcgatgacca actggcactg gacgttctgg accagcctcg tcctcagcgg
    11281 cgtcgtcgcc gccctttacg gcgccgtcgt cggcatgccg ctgctccggc ttcgcggcca
    11341 ctacttcgcg atcgcgaccc tcggtatggc ggagggcacc cgggagatag tgacgaacct
    11401 gcccgacctg accggcggcg gcggcggcat caccttgccc accgtcggcg cgggggccac
    11461 gacggcatac ctgggcaatg acgggttcta cctcgtcttt ctcgccctgg ccgccctcgg
    11521 gctgctggtc accggggtgg tgtcccggag caagttcggc ttcgccctgc gggcaatcca
    11581 ccaggacgag gatgccgcgg cggctgtggg gatcaacacg acgaagacca agatcctcac
    11641 cttcgccctg tccggattcc tcgcgggcct ggttggagcc acgtacgcct tccagcaggt
    11701 cacgatctac cccgagcgcc tcttcgacgt cgagatcacc gtgctcatgg tcgtcatggt
    11761 ggtcatcggc ggcagcggca ccgtcatcgg cccggtcatc ggggcggtcg ggctgcagtt
    11821 cctctccgag tggctccggc agaactacac cagcatccac accctgctcc tcggtgggat
    11881 catcattgtc gcggtgatcc tgcttcctca gggcatagcc aactacctca gcgaggccag
    11941 gcgcaccggg gagtactccc tcctggccaa cgtgaggagg taccggctat gagcggcgca
    12001 cccgtgctgg agacgcgcgg cctggggcgg cgcttcggcg gtctgaaggc ggtcgacgac
    12061 ctctcgatct cggtggccga agggctgacc ctcggggtca tcggccccaa cgggtcgggc
    12121 aagagcacct tcatcaacct cgtcaccggc cacctcaaac cgacgaccgg cagcgtgctc
    12181 atcgacggca cggatctgac cggcgccaag ccctgggtca tcgctgcagc tcgggtcgcg
    12241 cggaccttcc agatcgtcaa gcccttccgt ggcatgaccg ttcgccagaa cgtcgccgtc
    12301 ggcatcatgt acggccccga gggcaccgcc cggatggcac cggcccttcg ccaggccgac
    12361 gacgtactcg ccgaggtggg cctcgccggg tccggcgaca gggcgcccgg tgagctcacc
    12421 gtcgccgacg cccggcggct ggagttcgcc aaggccctcg ccttgcggcc acgcctcctt
    12481 ctgctcgacg aggtcatggc cgggcttcga ccggccgaga tcgaacccag cctcgccctg
    12541 atccagcagc tcaagcaggg ggggatgacc atcgtcgtgg tcgagcatgt gatgaaggcg
    12601 atcctcgcgg tgagcgacga ggtcatggtc ctgcaccaag gcgcggtgct catgcgtggc
    12661 tcgccgaggg aggtcctgtc cgacccccgc gtgatcgagg cctacctcgg acagaagtac
    12721 gccaagcgtc aggcgggcga gcatgcttga ggtcagcggg ctccaggcgg ggtatggccg
    12781 ggtccaggtg ctctgggacg tcgagatccg ggtcgggagc aaggagatcg tctccctcgt
    12841 cggacccaat ggtgccggca agaccaccct gctgcgcgcc ctctcgggca tggtccccgc
    12901 gaccgccggg acgatgacct tccgcggcaa ggacctgcgg gggctctcga tcgagcagat
    12961 cgtcgacctg gggatctctc acgtccccga gggtcggcgc ttgttccccg ggctgaccgt
    13021 gcgggacaac ctgcgtctgg gtggctggcg caaccgcaac cacaacctcg accgggtcat
    13081 cgagctcttc ccgaagctcg gcgagcggat caaccaggtg tgcggaacga tgtccggcgg
    13141 cgagcagcag atgtgcgcga tcggccgagg gctcatgggc gagccggagc tcatgatgat
    13201 cgacgagctc tccctcggcc tggccccggt cgtcgtcgag gagatcatcg cccggcttcc
    13261 cgacatctcg gcggccggga cctcggtgct gctggtcgag caggacgtcg acgccgcgct
    13321 gagcgccgcc gagcgcgcct acgtgctcga aaccggtcac atcgtcatga ccggcaaggc
    13381 cggcgagctc ctcgtcgacc cccggatcag ggcaagctac ctggggatcg cctgacgggc
    13441 gcgcggctgg gccaggagcc ggccagatcc acctcagtca gccacccccg caccaccgag
    13501 gccgatccga ccggccctga ccggccagat ccacctcagc cagccacccc ccggccacgg
    13561 cggccaggca ggcgggcagg accgggtcag cgatgaagtc acgttggcac ccgagtgccc
    13621 tgcaccgtcg ctctcagtac cgtcgctctc agtaccgtcg ctttcagtac cgtcgctttc
    13681 agtcctcgcg tcgggccctg gcctgcctag cacgtatggc gagccgctcc acgggcgggt
    13741 acgccacctc ctccaggcac aggccgtgcg cgggcatgac cgtgacccgg gggtctcggc
    13801 gcccggcgga caagacctcg gccggccacc cggtctcgac ccgcccttgc ccggccggca
    13861 ggacggcgcc gacgagtgag cggaccatcg agtggcagaa cgcgtcggcg cgcacactcg
    13921 cgacgagcac tccgtcgccg tcgcgtgccc aggcgaagcc gagcaaggtc cgtgtcgtcg
    13981 tcgccccctc acggcggcgg cagaacgcgg cgaagtcgtg cagcccgagc aaccccgcgg
    14041 aggcctcgtt cagcgc
//
LOCUS       DATAIX010000007         3172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_354, whole genome shotgun sequence.
ACCESSION   DATAIX010000007 DATAIX010000000
VERSION     DATAIX010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="VHM65_00135"
     CDS             <1..1310
                     /locus_tag="VHM65_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704142.1"
                     /translation="SALALLVCGLLLASVAMLVQRRQVRLVLVLAVGLGLVVAAAGPF
                     LAGGTLGSHPQLLANLGRLPLFRMVNGALPDTGTLVPAGFFGPGGQGIAFLVLLLSLQ
                     LVQAAKVMVWLPAVAALPRVRQDPMSWVLAGMALGGLGAVLTLFHPGGSALYFMYGAL
                     PFGSVLGAWLAAEALDEVGPDRSRVLARRSLLAGAGLSLLGAAVIWRLPVPSTGHGMT
                     TLSAVTMGVLVVLCLVIAAWVWRRGGFRDPARRQQSIGALAAGLVGAMVVSGPLLAVA
                     SPPSPRTPGASAKTGSVKPASPPPVSWVDRMRVGEYADSHLPDDALLATNAHCTEYAM
                     PTGCRAAQFWVSGFTLRQVLVSGWAYSTAANKDQTFTQPEALAKNDAFFSAPSRSGLD
                     YLRSQGVTYVVASLSNGPVSPAIASYADMVMRTPTVWLLKLKS"
     gene            complement(1316..>3172)
                     /locus_tag="VHM65_00140"
     CDS             complement(1316..>3172)
                     /locus_tag="VHM65_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX2704143.1"
                     /translation="AYGAATRGLSWRQIMAFYYPATTVSAAGNPSVRVRVSALGGAAT
                     TVVAAPGLTFTDGASTRTLVAANGAGVPIDRWRVIPDAGGMTLQWRTSGVWTSSTYWH
                     GWTRSSWAFSTPAGSVRVELPTATQRSYRGQVRTYVAGTSILPVNVVNMESYLPSVVP
                     AEMPASWAAGGLQSQAVAARTYAAYRLAHPLSSLYHLCDTTSCQVYHGAADYTSAGAL
                     AQTWEDARTNAAITATSGAILTYGGTAALTEFSASNGGMTAAGSAAYLVAKADPYDAA
                     VVSTSNPSSWSATLSLAQIEAAYPTIGTLKAISVPSRTRLGAWGGRMSSVQVVGTAAT
                     VTTTGSAFRAALGLKSEWWTMGSAPQTSAGTFPKDLTSDGLADLVAVDTAGALKVLKY
                     SGQMSFSTITAGTSGWSSMSLVTAVGPFDSDNRGDVLARSADGVLWLYRGGDTGVLYQ
                     SRTVAGLGWGGANLLVPVGDFNGDGHPDVLARGNDGALLLYSGNGSGKFTAVRAVSTG
                     WQVYASLFSPGDLTGDGVADLVGVKAADGAVLRFPGTGSGTLGPGTRIGNLASASTVW
                     GVGDLTGDGRPDLVARRRTDGALLIYPVMSTGTLDAPIALGTTWASYPTWAR"
BASE COUNT          450 a         1113 c         1099 g          510 t
ORIGIN      
        1 cctcggccct tgccctgctc gtgtgcgggc tgctcttggc aagtgtcgcg atgctcgtcc
       61 agcggcggca ggtccgactg gtccttgtac tggccgtagg gctcggcctg gtcgtggctg
      121 ccgccgggcc cttcctcgcc ggaggcaccc tgggttcgca tcctcagctg ctggccaacc
      181 tcggtcggct gccgctcttc cggatggtca acggcgccct gcccgacacg ggcacgctcg
      241 tgcccgccgg cttcttcgga cccggcggtc agggcatcgc cttcctcgtg ctgttgctca
      301 gcctgcagct cgtgcaggcc gccaaagtca tggtctggct gccggccgtg gcggcactgc
      361 ctcgggtccg tcaggacccg atgtcgtggg tgctcgccgg gatggcactc gggggactcg
      421 gtgccgtgct caccctcttc caccccggcg ggagtgcgct ctactttatg tatggcgcgc
      481 taccgttcgg cagcgtcctc ggcgcatggc tcgcggccga ggcgctcgac gaggtcggac
      541 ccgaccggtc gcgcgtcctc gcccggcgtt ctctcctggc cggggccgga ctttcgctgc
      601 tcggtgccgc cgtcatctgg cgtctgccgg ttccctcgac cggtcacggc atgaccacgc
      661 tctcggcggt caccatgggc gtcctggtcg ttctctgcct ggtcatcgca gcctgggtat
      721 ggcggcgagg tggcttccgg gaccccgccc ggcgtcagca gtccatcggc gccctcgccg
      781 cggggctggt gggcgccatg gtcgtgagcg gaccgctgct ggcggtggcc tcgcctccgt
      841 cgccgcgtac ccccggggcg tcggcgaaaa cggggagtgt caagccggcg tcgccacccc
      901 ccgtgtcctg ggtcgaccgg atgagggtcg gtgagtatgc cgacagccac ctcccggacg
      961 acgcactgct cgcgaccaac gcccactgca ccgagtacgc catgcccacc gggtgccgag
     1021 ccgcgcagtt ctgggtcagc ggcttcacgc tacgtcaggt gctcgtctcc ggatgggcct
     1081 actcgacggc agctaacaag gaccagacct tcacccagcc ggaggcactc gccaagaacg
     1141 acgccttctt ctcggccccg agccgaagcg gacttgacta cttgcgctcg caaggggtga
     1201 cgtatgtcgt cgcctcgctc agcaacgggc ccgtctcgcc cgccatcgcg agctatgccg
     1261 acatggtcat gcgcacccct acggtctggt tgctcaagct caagtcctga gctgctcacc
     1321 gggcccacgt cgggtaagag gcccacgtcg tcccgagcgc aatcggagcg tccagggtgc
     1381 ccgtgctcat gacggggtag atgagcaggg caccgtccgt ccggcgccgg gcgaccaggt
     1441 caggacgtcc gtcaccggtg aggtccccga caccccagac tgtcgaggcc gaggcgaggt
     1501 tgcctatgcg cgttcctggg cccagcgtgc cggatcccgt gcccgggaac ctcagcaccg
     1561 cgccatcagc ggctttgaca ccgacgaggt cggcgacacc gtcgccggtc aggtcacctg
     1621 gggagaacag cgaggcgtag acctgccacc cggtcgagac ggctcggact gcggtgaact
     1681 tgcctgaccc gttgcccgag tagagcagca acgcaccgtc gttgccgcgg gccaggacgt
     1741 ctgggtgtcc gtcaccgttg aagtcgccga cgggcacgag aaggttggcg ccgccccagc
     1801 ccaaccccgc gacggtccgc gactgataga gcacgcccgt gtcgccgccc cggtagagcc
     1861 acagcactcc gtcggccgag cgagccagca catcgccacg gttgtccgag tcgaagggcc
     1921 cgacggccgt gacgaggctc atcgatgacc accccgacgt ccccgccgtg atggtcgaga
     1981 agctcatctg gccggagtac ttgagcacct tgagagcgcc cgccgtgtcg accgcaacga
     2041 ggtcggccag accgtcggag gtcaggtcct tggggaacgt cccggccgag gtctgcggcg
     2101 ccgaccccat ggtccaccat tcgctcttca gacccagggc ggcccggaaa gccgacccgg
     2161 tcgtggtcac cgtcgcggcg gtcccgacca cctgcacgct gctcatccgg ccaccccagg
     2221 caccgagccg agtccgggag ggcacggaga tcgccttgag ggtgccaatc gtggggtagg
     2281 ccgcctcgat ctgggccagg ctgagggttg ccgaccacga gcttgggttg gaggtcgaga
     2341 cgacagcggc gtcatacggg tcggccttcg ccacgaggta cgccgccgag ccggcggccg
     2401 tcatacctcc gttcgatgcc gagaactcgg tgagcgcggc agtcccgccg taggtgagga
     2461 tcgcgccgga ggtagccgtg atcgcggcgt tcgtccgtgc gtcttcccag gtctgggcca
     2521 gtgccccagc tgaggtgtag tccgccgcgc cgtggtaaac ctggcagctg gtcgtgtcgc
     2581 acaggtggta gagcgacgac agcgggtgcg caagccggta tgcggcgtac gtgcgggccg
     2641 cgacggcctg ggactgcagg ccgccggccg cccaggaggc cggcatctcc gcagggacga
     2701 cgctgggcag gtaggactcc atgttgacga cgttgacggg caggatcgag gtccctgcca
     2761 cataggtgcg gacctgaccg cgatagctgc gctgtgtcgc ggtgggcagc tcgacgcgga
     2821 ccgaacccgc gggggtactg aacgcccagg acgagcgggt ccagccgtgc cagtaggtgc
     2881 tcgaggtcca cacaccggac gtccgccact gcagggtcat gcctcccgcg tccgggatga
     2941 cccgccaacg gtcgatcggc actccggcgc cgttggctgc cacgagagtt ctcgtgctcg
     3001 ctccgtcggt gaaggtcaag cccggtgcag caacgactgt cgtggccgcg ccaccgagtg
     3061 ccgagactcg gacccggacc gagggattgc ctgctgccga cacggtggtg gcggggtagt
     3121 agaacgccat gatctggcgc caggagaggc ccctggtcgc cgcaccgtag gc
//
LOCUS       DATAIX010000008         2745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_119364, whole genome shotgun sequence.
ACCESSION   DATAIX010000008 DATAIX010000000
VERSION     DATAIX010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119364"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1336)
                     /locus_tag="VHM65_00145"
     CDS             complement(<1..1336)
                     /locus_tag="VHM65_00145"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF006681.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEX2704144.1"
                     /translation="MTSYWAELAFLPAGLARRVRLVVDGERFGAVTSGSDPQPGDERL
                     AGVVLPGLANGHSHAFHRALRGRTHAGGGSFWTWRERMYDVARRLDPDTYLTLARAAY
                     AEMALAGYTVVGEFHYLHHGPGGVAYEDRNAMGHALIQAASDAGVRLTLLDTCYLAGG
                     LGPDGHLPLDEVQRRFGDLSVDRWADRVADLRSADRVRIGAALHSVRAVPREGLAALA
                     AVARHGAGSGATHRGMPVHAHVSEQPAENAAALAYYGLTPVQLLAAEGVLGPGFTAVH
                     ATHLTSEDVGLLGASGCAVCLCPTTERDLADGIGPARALRRAGALLTVGSDQHAVIDP
                     FEEIRGLEMHERLASLERGCFVPAELLLAGAGSGYRSLGWDSGGALCEGGLADFVAVG
                     LTSPRTAGSQPGQVLLSATSADVTDVVVGGRRVVTDRQHRLGDVGQLLADAIH"
     gene            complement(1341..2543)
                     /locus_tag="VHM65_00150"
     CDS             complement(1341..2543)
                     /locus_tag="VHM65_00150"
                     /inference="COORDINATES: protein motif:HMM:NF006770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HEX2704145.1"
                     /translation="MTFEPMWSAIEPIGRHHRTGGYRRFAWTREDHDLREWFAGEAAA
                     RGMDLYVDRAANQWAWWGDPDAAVAAGRPGLAIGSHLDSVPDGGAFDGPLGVVSAFAA
                     VDALRRAGIEPVRPLAVVSFVDEEGARFGVACAGSRLLTGVLDPDRARALTDADGVSQ
                     ADAMRTAGHDPMHLGPDPEALRRVGTYVELHIEQGRALAGLGRPVAVGTSIWPHGRWR
                     LDLHGEANHAGTTRLVDRHDPMLGLADVVRSARAAAERHGCVATVAKVRVHPNGVNAI
                     PSQVTAWLDARGALPEDVRAVVADVAGTAQPWAPAGAVEESWTPVTDFDPALVSRLVA
                     LLDGAPVLGTGAGHDAGILATAGIPAAMLFVRNPTGVSHAPGEHAEAADCFAGVGALA
                     TVVADLVR"
BASE COUNT          352 a         1013 c         1070 g          310 t
ORIGIN      
        1 ggtgtatggc gtccgcgagc agctgcccga cgtcaccgag gcggtgctgc cggtcggtga
       61 cgacgcgccg gccgccgacg acgacgtcgg tgacgtcggc ggaggtcgcg ctgagaagga
      121 cctgcccggg ttggctcccg gccgtcctcg ggctggtcag cccgacggcg acgaagtcgg
      181 cgagcccacc ctcgcacagc gctccgccgg agtcccagcc cagcgagcgg tagccgctcc
      241 cggcgcctgc caggagcagc tcggcgggca cgaagcagcc gcgttcgagc gaggccagcc
      301 gctcgtgcat ctccagcccg cggatctcct cgaacgggtc gatgaccgcg tgctggtccg
      361 agccgacggt gagcagcgcg ccggcccggc gcagggcccg ggcggggccg atcccgtcgg
      421 cgaggtcgcg ctcggtcgtc ggacacagac acacggcgca gccactcgcg ccgaggagcc
      481 cgacatcctc gctggtgagg tgggtggcgt ggactgcggt gaagcccggc ccgagcaccc
      541 cctcggcggc gaggagctgc accggggtca ggccgtagta ggccagcgcc gcggcgttct
      601 cggccggctg ctcgctcaca tgggcatgga ccggcatccc gcgatgggtc gcgccggacc
      661 cggccccgtg ccgggcgacc gccgccagcg cggcgagccc ctcgcgcggc acggcccgga
      721 ccgagtgcag ggccgcgccg atccggaccc gatcggccga gcgcaggtca gccacccggt
      781 ccgcccaccg gtcgacggag aggtcaccga agcgccgctg gacctcgtcg agcgggaggt
      841 gaccgtcggg cccgagcccg cccgcgaggt agcacgtgtc gagcagggtg agccggacgc
      901 ccgcgtcgga cgccgcctgg atgagggcgt ggcccatggc gttgcggtcc tcgtaggcga
      961 cgccgcccgg tccgtggtgc aggtagtgga actcgccgac gaccgtgtag ccggccagcg
     1021 ccatctcggc gtatgccgcc cgcgccagcg tgaggtaggt gtcgggatcg aggcggcgcg
     1081 cgacgtcata catgcgctcc cgccacgtcc agaagctgcc gccgcccgcg tgggtgcgtc
     1141 cgcgcagggc ccggtggaag gcgtggctgt ggccgttggc caggcccggg aggacgacac
     1201 cggcgagccg ctcgtcgccg ggctgcgggt ccgaacccga cgtgaccgcg ccgaagcgct
     1261 cgccgtccac gacgagccgg acgcgcctcg ccagcccggc gggcaggaag gccagctcgg
     1321 cccagtatga cgtcaccggc tcacctcacg aggtcggcga cgacggtcgc gagcgcgccg
     1381 accccggcga agcagtcggc cgcctcggcg tgctcgccgg gggcgtgcga gacgccggtg
     1441 gggttgcgga cgaagagcat ggccgcgggg atgcctgccg tggcgaggat cccggcgtcg
     1501 tgcccggcgc cggtgccgag gacgggggcg ccgtcgagca gagcgaccag ccggctcacc
     1561 agcgccgggt cgaagtcggt gaccggggtc caggactcct cgaccgcgcc ggcgggcgcc
     1621 cacggctggg cggtcccggc gacgtcggcg acgaccgccc ggacgtcctc cggtaaggcg
     1681 ccgcgggcgt cgagccaggc ggtgacctgg gacgggatcg cgttgacgcc gttggggtga
     1741 acgcgcacct tggcgaccgt ggcgacgcag ccgtggcgct cggcggcggc ccgggccgac
     1801 cggacgacgt cggcgaggcc gagcatcggg tcgtggcggt ccacgagccg ggtcgtcccg
     1861 gcgtggttgg cctctccgtg caggtcgagg cgccagcggc cgtgcggcca gatcgaggtc
     1921 ccgacggcga ccggccggcc gaggccggcc agagcgcggc cctgctcgat gtgcagctcg
     1981 acatacgtgc cgacccggcg cagcgcctcg gggtccgggc cgaggtgcat cgggtcgtgg
     2041 cccgcggtgc gcatcgcgtc ggcctgcgag accccgtcgg catcggtgag ggcccgggcc
     2101 cggtcggggt cgaggacacc ggtgaggagg cgcgacccgg cgcaggcgac cccgaaccgg
     2161 gcgccttcct cgtcgacgaa gctgacgacg gccagcggac ggaccggctc gatcccggcc
     2221 cggcgcagcg cgtccacagc ggcaaacgcg ctgaccacgc cgagcgggcc gtcgaaggca
     2281 ccgccgtcgg gcaccgagtc caggtgcgag ccgatcgcca gccccgggcg gccggcggcc
     2341 accgccgcgt cggggtcgcc ccaccaggcc cactggttgg ccgcccggtc cacatacagg
     2401 tccatcccgc gggccgctgc ctcgcccgcg aaccactccc gcaggtcgtg gtcctcgcgg
     2461 gtccacgcga agcgccggta gccgccggtg cggtgatgac ggccgatcgg ctcgatcgcg
     2521 gaccacatcg gctcgaacgt catggcccca acctatgctc gatgcgcccg cacgtgaccg
     2581 atgtcactgg ccctctcgca tcggcacccg gatgcccgtg gccgcggcga cgtcctcggc
     2641 gagctggtag cccgcgtcga cgtggcggat gacgcccatg cccgggtcgt tggtcaggac
     2701 gcgggcgagc ttggccgccg cgagctcggt gccgtcggcg acgca
//
LOCUS       DATAIX010000009         4170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_89879, whole genome shotgun sequence.
ACCESSION   DATAIX010000009 DATAIX010000000
VERSION     DATAIX010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89879"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VHM65_00155"
     CDS             complement(<1..552)
                     /locus_tag="VHM65_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEX2704146.1"
                     /translation="MDNTPPAGSRRTHPLRILLLCSSFNGLCQRVWTELRAAGHRVTV
                     QLAADEPTLKAAVDAADPDLVLCPFLRERIPETVWRARPTVVIHPGPKGDRGPSALDW
                     AIMGGEQVWGATAVQAVEEMDAGPIWASRDFPVPADPPRKSALYNGPITDTAMQLINE
                     VITKAADPDFRPEPLDYTRPDVWG"
     gene            complement(975..1808)
                     /locus_tag="VHM65_00160"
     CDS             complement(975..1808)
                     /locus_tag="VHM65_00160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2704147.1"
                     /translation="MTLQTDPAYVFERSSDEYMRLTRQAALTEAMTERLFRDAGICPG
                     MRVLDVGSGVGDVALLAARLVGPHGSVVGIDIDGTALQVARARAGTLGLQNLTFNEGD
                     VRSAETGAHFDAAVGRLVLGYLADPAEALRAIAERVHPGGIVAFQELDLDPGVPSRSF
                     PEGTLWDQTGRLVIETFARAGTHVRMGRQLYGSYLAAGLPAPAMREEAIVGAGPGFAG
                     YAWLAGVARSLAPLMARLGIDDAQQLDLDTLAERLRDDAVARGAVVWGPALIGAHARR
                     P"
     gene            1994..>4170
                     /locus_tag="VHM65_00165"
     CDS             1994..>4170
                     /locus_tag="VHM65_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2704148.1"
                     /translation="MTRGRGADAVSGDLWVGSLLERERELGLLERSLADAERARGRLV
                     VVDGPAGIGKTSLLRAAAELARGRGLTVLAARGAPLEQNFSFGAVRQLFEPHSLPSGR
                     RADDDILTGAATLALRALGEGEPGRDSTAEDLSFSTLHGLYWLTANLASRAPLLLIVD
                     DCQWADGASLRFLAHLGARLDGLRVVVLVALRSGDPGAYPDLVGEVRSLATGEPIRPS
                     PLGTGAAAFLVRGDLESATDLYCRACHDATGGNPLLLQSLAAAVAAEGIEPNDEAAAR
                     VTSYGAQSISRQLARRLALLPTGSDAFVRALAVLGQRSPLRHLASLATLDLERAADIA
                     DALRAGSILAPSLELDFAHPILRVAADEAMGPDERALAHARAAKLLADDGAPPDRLAL
                     HLLHAHPRGDAAVVAKLRAAAAVASGRGAPETAATCLRRALEEPPPRALRGPLLLELG
                     LAQMAARRDPLAVAGFREAITLIEMPPDRLAAALRAGRALGVAGYFAEAVATLEQVPE
                     FDLRIEAELAANRCQITAQEPAALAHLAQYRATDLPPGEGRRLMLVMLAHRSLCTGDP
                     AAVTADLLERARQGPELFAEESLVTVYAAMDLVLVDRFDDAERLCTAVIEDGQRRGAV
                     STVATFAFPRAFAFLRRGMLRDAEAEARWSFEQKMAMGAEQGPPWPLACLVDCLTELG
                     DFTGADAALAKVSTDKTGQPEMLAWAFLVEARGRLRIAQGRMR"
BASE COUNT          580 a         1480 c         1483 g          627 t
ORIGIN      
        1 gccccagacg tcgggacggg tgtagtccag cggttcgggg cggaagtccg ggtcggcggc
       61 tttggtgatg acctcgttga tcagttgcat cgcggtgtcg gtgatggggc cgttgtacag
      121 cgcgctcttg cgcggcgggt ccgccgggac gggaaagtca cgggacgccc agatcgggcc
      181 ggcatccatc tcctcgaccg cctgaaccgc ggtcgccccc cacacctgtt caccgcccat
      241 gatggcccaa tccagggccg acgggccccg gtcgcctttc ggtcccgggt ggatgacgac
      301 cgtcggacgg gcccgccaca ccgtttccgg gatgcgctcc cgcaggaacg ggcagagcac
      361 caggtcgggg tcggcggcgt ccaccgccgc cttcagggtc ggctcgtcgg ccgcgagctg
      421 cacggtgacc ctgtgcccag ccgcacgcag ctcggtccag acccgctggc acaagccgtt
      481 gaacgacgaa cacagcagca ggatccgcag cgggtgcgtg cggcgggaac cggctggcgg
      541 tgtgttgtcc atatcagcgg gagtgggagc acgatcgctc cgataccggc cggcagccac
      601 cgatcgccgg gtcgcggcga atgggcgtgg tatgcggcgc ccgcgcaaac gggcggtgtt
      661 caccgcgcct ggccatcggg gaccattggc gagagttctt ccgcgccttc gagatgggct
      721 cgccgccggg cggagaagaa cgcgccgcgc gccggccgag ccggcgcgaa cgcgagcagc
      781 ccgagcgagg agcccgtcgg gtcgggccgc ggctgcacgc tcgaattgct tcccgagcga
      841 gcggagcgtg cccagcccac gtggcgtgcg cacacacatg ccgaggtaga tgacaacagg
      901 atcgggaaac gcggtcagat cgaccgttgt ccggctgacg tcgcctccgc gagcagcctg
      961 cggggcgccg cgcatcacgg cctccgtgcg tgggcgccga tgagggccgg cccccagacg
     1021 accgcgccgc gggcgaccgc gtcgtcacgg aggcgctccg cgagcgtgtc caggtcgagc
     1081 tgctgcgcgt catcgattcc cagacgtgcc atgagcgggg cgagactacg ggcgacgccg
     1141 gccagccatg cgtacccggc gaaaccgggg ccggcgccca cgatcgcttc ctcccgcatc
     1201 gcgggcgcag gcaacccggc ggcgaggtac gagccgtaaa gctgccgccc catgcgcacg
     1261 tgggtgccgg cacgggcaaa cgtctcgatc accagccggc cggtctggtc ccagagcgtc
     1321 ccctcgggga acgatcgcga gggcacaccg gggtcaaggt cgagctcctg gaacgcgacg
     1381 atcccacccg ggtggacgcg ctcggcgatc gcgcgcaatg cctcggccgg gtctgccagg
     1441 tagcccagca cgagccgccc gaccgccgcg tcgaagtggg ctccggtctc cgcggagcgg
     1501 acgtcgcctt cattgaatgt caggttctgt aaacccagcg tcccggctcg agcccgtgcg
     1561 acctgcagag ccgtaccgtc gatgtcaatg ccgacgacgg agccgtgcgg cccgacgagc
     1621 cgggcggcga ggagcgcgac gtctccgacg ccgctgccga cgtcgagtac ccgcatacct
     1681 gggcagatgc cagcgtcgcg gaagagcctc tctgtcatcg cttcggtgag cgccgcctgc
     1741 ctggtcaggc gcatgtattc atcgctggag cgctcgaaaa cataggcggg atcggtctgt
     1801 agggtcatcg gctcctcatt gggtcgtggt ggggcgctgc cctcagcggt gctgctcggg
     1861 cgggacggcc catgccccga gggtgcgcgc ggcgtggccg tcgggcatcg tggctgctgc
     1921 cctgagcttt tgccccttgt ctactgagga tttcgcccct tcgggctcgc tccagtactg
     1981 ccacaatcgg gtggtgacgc gcggacgcgg cgccgacgcg gtttccggcg acctctgggt
     2041 cggctccctg ctcgagcggg agcgcgaact cggactactc gagcgcagcc tcgcggatgc
     2101 ggagagggcg cgggggcgcc tcgtcgtcgt cgatggtccg gcgggcattg gtaagacgtc
     2161 gctgttgcgc gccgcggcgg aactcgcccg gggtcgcggt ctgaccgttc ttgccgcacg
     2221 cggcgcaccg ctcgagcaga acttctcctt cggcgctgtc cgccaactat tcgagccgca
     2281 ctccctgccg agcggcaggc gggccgacga cgacatcctc accggtgccg ccaccttggc
     2341 actgcgggcg ttgggagagg gggaacccgg ccgagactcc acggccgaag acctatcttt
     2401 ctcgaccctg catgggctgt actggctgac cgcaaacctc gcctcgcggg caccgctgct
     2461 cctcatcgtt gacgactgcc agtgggccga cggcgcgtcg ctgcggttcc tcgcccacct
     2521 cggggcgcgc ctcgacgggt tgcgggtggt tgtcctcgtc gcgttgagga gtggtgatcc
     2581 gggcgcctac cccgacctgg tcggtgaggt ccgctcgctc gcgaccggtg agccgatccg
     2641 gccgtcgccg ctgggaaccg gtgcggcggc gtttctcgtc cgcggcgacc tcgagtcagc
     2701 caccgatctg tactgccgtg cctgccacga tgcgaccggc ggaaacccct tgctcctgca
     2761 gtcgctcgcg gcggcagtgg ccgcagaggg gatcgaaccg aacgacgagg ccgcggcccg
     2821 ggtcacaagt tacggcgccc agagcatttc ccgccaactc gcgcgacggc tggccctgct
     2881 tccgacgggg tcggacgcct tcgttcgtgc gctcgctgtt ctgggtcagc gcagtccgct
     2941 caggcatctc gcgagccttg ccacgctcga cctcgaacgc gcggccgaca tcgccgatgc
     3001 gctgcgtgcc gggtccatcc tggccccgtc gctcgaactc gactttgccc acccgatcct
     3061 ccgtgtcgcc gccgacgagg cgatggggcc cgacgagcgg gcactcgctc acgcgagggc
     3121 ggcgaagctg ctcgcagacg acggtgcccc gccggaccgg ctcgccctgc atctcctgca
     3181 cgcccatccg cgcggcgacg ccgcggtcgt cgcaaagctg cgtgcggcgg ccgccgtcgc
     3241 ctcgggccga ggcgcacccg agaccgccgc aacctgccta cggcgggcgc tcgaggagcc
     3301 gccgccgaga gcgttacggg ggccgctgct actggaactc ggcctggcgc agatggctgc
     3361 cagacgcgac ccgctcgccg tcgccggatt tcgcgaggcg atcacgctga tcgagatgcc
     3421 gcccgaccgg ctcgccgcgg cgctccgcgc cggacgcgcg ctcggcgtgg cgggctactt
     3481 cgccgaggcc gtggccaccc tcgaacaagt gcccgagttc gacctgcgga tcgaggcgga
     3541 gctcgccgcg aaccgctgcc agatcaccgc ccaggaaccc gcagcgctcg cacacctcgc
     3601 ccagtaccgt gccactgatc tgccaccggg cgagggccgc cggttgatgc tggtgatgct
     3661 ggcccatcgc agcctgtgca ccggtgatcc ggcggccgtc accgcagatc tgctcgagcg
     3721 agcccggcag ggcccggagc tcttcgccga ggagtcgctg gtgaccgtct acgccgccat
     3781 ggacctagtg ctggtcgatc ggttcgacga cgccgaacgg ctgtgcaccg ctgtcatcga
     3841 ggatggtcag cggcgaggcg ccgtcagcac cgtggcgaca tttgccttcc ctcgagcgtt
     3901 cgccttcctg cgccggggca tgctgcgcga cgcggaggcc gaggcccgat ggtcgttcga
     3961 gcagaagatg gccatgggtg cggaacaagg cccgccgtgg cccctggcgt gcctcgtcga
     4021 ttgcctcacc gagctgggcg acttcacggg cgccgacgca gcacttgcga aggtgtccac
     4081 cgacaagacg ggacagcccg agatgctcgc ctgggcgttc ctggttgagg ctcgcggtcg
     4141 tctgcgcatc gcgcagggca ggatgcgtga
//
LOCUS       DATAIX010000010         4774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_178989, whole genome shotgun sequence.
ACCESSION   DATAIX010000010 DATAIX010000000
VERSION     DATAIX010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178989"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VHM65_00170"
     CDS             complement(<1..304)
                     /locus_tag="VHM65_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704149.1"
                     /translation="MTARTHTPVTVISPARRLALAQRAQLLATASVTYNVAEAVVAIS
                     AGTVAGSTALIGFGLDSTVEVASGLVILWQFRHPMPETRERRAQRLIAVSFFALAGY"
     gene            complement(301..1200)
                     /locus_tag="VHM65_00175"
     CDS             complement(301..1200)
                     /locus_tag="VHM65_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX2704150.1"
                     /translation="MSGGHGHGHAGSRHRWRLAVAFVLVAGFFGVELVAGLVSGSLAL
                     LSDAGHMAADVVTLGAALVATRIAVRKDTSGRRTFGSYRAEVFASGLAVLVMAGVAVY
                     IGIEAIGRLGSAPGVASGTMLLVGAIGLVVNLIALLMLRGGAGESLNVKGAYLEVVAD
                     TVGSFGVIAAALLIRWTGDAWWDTAIALAIAVFVLVRAVMLGREVLAVLGQHAPADVE
                     PTEMVEALSAVHDVLDVHDLHVWSLTSGMTVASAHLVARDQADPAAVLDTAQEVLRDR
                     FSIAHATLQVEAAGRAQCQGATW"
     gene            complement(1197..1529)
                     /locus_tag="VHM65_00180"
     CDS             complement(1197..1529)
                     /locus_tag="VHM65_00180"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2704151.1"
                     /translation="MRSKAPGEAELGRVTRVFKLLGDPTRARLLYALLEAGELCVCDL
                     AAATRAAEATVSQSLRLLRSSGVVTGRREGRNVFYRLSDAHVRLLLDLTREHALHEAP
                     GAARGAAR"
     gene            complement(1745..2725)
                     /locus_tag="VHM65_00185"
     CDS             complement(1745..2725)
                     /locus_tag="VHM65_00185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX2704152.1"
                     /translation="MTPGPRDHGHHGLWWTRLRNAVRPHSHDAAGSLDAALESSADGI
                     RAVKVSLVGLGATALLQVAVVLVSGSVALLADTIHNFSDALTALPLWLAFVVGRRAPT
                     RRYTYGYGRAEDLAGLFVVFMIALSALLAGYAAVRRLAEPAPVEHLGWVAAAGLAGFA
                     GNELVALYRIGAGRRIGSAALIADGLHARTDGLTSLAVVLGAAGVALGFPLADPVVGL
                     LITGAIVLVLWSAARDIYGRLMDAVDPALVDQAEAALRGMPGVVSVRRVRMRWLGHQV
                     QADADLDIAPTVTLDEAHRLAHTAERRLLDAVPKLSLAVVHAYPRQAGHD"
     gene            complement(2722..3135)
                     /locus_tag="VHM65_00190"
     CDS             complement(2722..3135)
                     /locus_tag="VHM65_00190"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2704153.1"
                     /translation="MNADTDSRGLPLEQAELAVEVFRMLADATRVRLLWALLDEELSV
                     SDLAEQVGKPAPAVSQHLAKLRLARLVRTRREGTQVIYRLENDHVARLVRDAVHNAEH
                     AGPEVPRHHQGQAVAVRTTAAGGVRPQGGARGVRR"
     gene            3448..>4774
                     /locus_tag="VHM65_00195"
     CDS             3448..>4774
                     /locus_tag="VHM65_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX2704154.1"
                     /translation="MTYPKVRTDDTAPGPSGAPEGVAASPRFPDIETRVLRYWAQDGT
                     FAASVEARDAGEDGSNEFVFYDGPPFANGLPHYGHLLTGYVKDIVPRYETMRGHRVER
                     RFGWDTHGLPAELEAQRQLGLKTKKDIHRIGIERFNAACRESVLRYTSEWQEYVTRQA
                     RWVDFEHDYKTMNPEFMESVIWAFKSLYDKGWVYEGQRVLPYCWQDETPLSNHELRMD
                     DEVYQQRQDPSVTIWAELDSGERLLAWTTTPWTLPGNLAMAVGPDIDYVTVEHEGQRY
                     VLAEARLASYTRELGEDAATRVVARVKGAQLVGRRYAPLFPYFSNVDVWGTAQAFTVI
                     ASEETTTEDGTGIVHMAPAYGEVDQLECEANGIPTILTVDERAVFTSVVPDYAGLQVF
                     EANRPIINDLRAAGRLMREESYVHSYPHCWRCRTPLVYKAVSSWFVQVTA"
BASE COUNT          770 a         1708 c         1676 g          620 t
ORIGIN      
        1 catagccggc gagggcgaag aaggacacag cgatcaggcg ctgcgcgcgc cgctcccggg
       61 tctcgggcat gggatggcgg aactgccaca ggatgaccag cccgctggca acctcgaccg
      121 tggagtcgag gccgaagccg atcagggcgg tcgagccggc cacggtgcct gcggagatgg
      181 caacgaccgc ctcggcgacg ttgtaggtca ccgaggccgt ggccaggagc tgcgcgcgct
      241 gggccagtgc tagccgccgc gccgggctga tgaccgtgac gggggtgtgg gtgcgggcgg
      301 tcaccatgtc gccccctggc actgggcgcg ccccgcggcc tcgacctgaa gtgtcgcgtg
      361 ggcgatcgag aaccggtcgc gcagcacctc ctgggccgtg tcgaggaccg cggcggggtc
      421 cgcctggtcc cgcgcgacga ggtgcgcgct ggccaccgtc atcccggagg tcaggctcca
      481 cacgtgcaga tcgtggacgt ccagcacatc gtgcacggca ctgagcgcct ccaccatctc
      541 cgtgggctcg acatcggcgg gcgcgtgctg gcccagcaca gccaggacct ctcggccgag
      601 catgactgct cgcacgagga cgaacaccgc gatggccagg gcgatggccg tgtcccacca
      661 ggcgtcgccg gtccaccgga tcagcagcgc cgcggcgatc acaccgaacg agccgacggt
      721 gtcggccacg acctcgaggt aggccccctt gacgttgagg ctctccccag cgcccccgcg
      781 cagcatgagg agggcgatga ggttcaccac aagcccgatt gcgccgacga gcagcatcgt
      841 gccgctcgcc acccccggcg ccgagcccag ccgcccgatg gcctcgatgc cgatgtacac
      901 ggcgacgccg gccatgacca gcacggccag ccctgacgcg aacacctccg cgcggtagga
      961 gccgaaggtg cgcctgcccg aggtgtcctt gcggaccgcg atccgggtcg cgacaagagc
     1021 tgcgccgagg gtgacgacgt cggcggccat gtggcccgcg tccgagagca gggccaggga
     1081 gcccgagacc agtccggcga cgagctcgac gccgaagaac ccggcgacca ggacgaaggc
     1141 gacggccagc cgccaccggt gccggctccc cgcgtggccg tgaccgtgtc caccactcat
     1201 cgcgctgccc cgcgtgcggc cccgggagcc tcgtgcagcg cgtgctcacg ggtcaggtcg
     1261 agcaacagcc ggacgtgggc gtcggagagc cggtagaaga cgttgcgccc ctcacgccga
     1321 ccggtgacga ccccggagga acgcagcagc cgcagcgact gcgagaccgt cgcctccgcg
     1381 gctcgcgtgg ctgccgcgag gtcacagacg cagagctccc cggcttcgag cagcgcgtag
     1441 aggagccggg cgcgggtcgg gtcgccgagc agcttgaaca ccctggtcac ccggccgagc
     1501 tctgcctccc ccggcgcctt cgaccgcacc tgctcgacct tgtccgggtg cacacacgcg
     1561 gtcgcgcacc cgtcaagccc catcagggtt gtcacaagac acctcgtcat ctgatcgttt
     1621 gctcagatgt aagtctcagt cgtggaccgc gacgtgtcaa tggccgcggg gacgaggcgc
     1681 aggctcaccg ggcgtgcggc cgcctccaca gccaggagat gtccgccagc cagtagtggg
     1741 cggctcagtc gtggccggcc tgcctcgggt aggcatgcac cacggcgagg ctcagcttcg
     1801 ggactgcgtc caggagacgc cgctcggcgg tgtgcgccag ccggtgcgcc tcgtccaggg
     1861 tgacggtggg cgcgatgtcc aggtcagcgt ctgcctggac ctggtgtccc agccagcgca
     1921 tccggacccg gcgaacggac acgacacccg gcatgccccg cagcgcggcc tcggcctggt
     1981 cgaccagagc ggggtcgacg gcatccatga gccgaccgta gatgtcccgt gccgcagacc
     2041 acagcacgag cacgatggca ccggtgatga gcagacccac gacggggtcg gccagcggga
     2101 agccgagagc gacgccggct gcgccgagta cgacggccag cgacgtgagg ccatcggtgc
     2161 gcgcgtgcag tccatcggcg atcagcgcgg ccgagccgat gcgccgcccg gccccgatcc
     2221 ggtagagggc cacgagctcg ttgcccgcga agccggccag gccggccgcc gccacccacc
     2281 cgaggtgctc cacgggagcc ggttcggcca ggcggcgcac cgccgcgtag ccggcgagca
     2341 gggcggacag cgcgatcatg aagaccacga acagcccggc aaggtcctcg gcccgcccgt
     2401 agccgtaggt gtagcgccgg gtcggggccc gccggccgac gacgaacgcc agccatagcg
     2461 gcagggcggt cagcgcgtcc gagaagttgt ggatcgtgtc ggcgagcagt gccaccgagc
     2521 ccgagacgag cacaaccgcc acctgcaaca gcgccgtggc cccgagcccc accagactga
     2581 ccttgacggc ccgtatgccg tccgcgctgg actcgagtgc agcgtcgagt gaccccgcag
     2641 catcatggga gtgcggtcgc acggcgttgc gcagccgggt ccaccagagg ccatgatgac
     2701 catggtcacg aggtcccggg gtcatcgcct cacaccccga gcgccgccct gcggcctcac
     2761 acccccggca gcggtcgtgc gcactgccac ggcctggccc tgatggtggc gggggacctc
     2821 gggaccggcg tgttcggcgt tgtgcacggc atcccggacc aggcgcgcca cgtggtcgtt
     2881 ctcgagccgg tagatgacct gggtgccctc gcggcgggtt cgcaccagac gcgccagccg
     2941 cagcttggcc agatgctgcg acaccgcggg ggcgggcttc ccgacctgct cggcgaggtc
     3001 gctgaccgac agctcctcat cgagcagcgc ccacagcagc cgcacccggg tcgcatcggc
     3061 cagcatccgg aacacctcca cggccagctc ggcctgctcc aggggcagac cccgggagtc
     3121 agtatctgca ttcatgcata gatagtaagg cagtttggtg tggcggcggt tccggtacac
     3181 ttcccggaca ggcgtctgag ccgtcatcag cggcgagcct tccggaagaa cgggaccacg
     3241 cgccataccg gctggtcctc actagacccg gacgggtggc ccgtcacagc cacgaacgag
     3301 cggccgtatg ccgtgcgctc accccggggt tccgcctcgc cgagcgcggt gcgtgcggca
     3361 agcgaggtgg taccgcggtg ccgccggggc ctgtcccggc gctgtcgtcc tcgtgtgcgg
     3421 cacacgagct gacggagcac cacgacgatg acgtacccca aggtccgcac cgacgacacc
     3481 gcacccgggc catcgggcgc gcccgagggc gtcgcggcga gcccgcgctt cccggacatc
     3541 gagacgcggg tgctgcgcta ctgggcccag gacgggacgt tcgcggcctc ggtcgaggcg
     3601 cgcgacgcgg gcgaggacgg gtccaacgag ttcgtcttct acgacggtcc gccctttgcc
     3661 aacgggctgc cgcactacgg ccacctgctc accggctatg tcaaggacat cgtgccgcgc
     3721 tacgagacga tgcgcggcca ccgtgtcgag cggcggttcg gctgggacac ccacgggctg
     3781 ccggccgagc tcgaggccca gcgccagctc gggctcaaga cgaagaagga catccaccgg
     3841 atcgggatcg agcgcttcaa cgcggcctgc cgcgagtcgg tgctcaggta cacctcggag
     3901 tggcaggagt acgtcacccg ccaggcgcgc tgggtcgact tcgagcacga ctacaagacg
     3961 atgaacccgg agttcatgga gtcggtgatc tgggcgttca agtcgctcta tgacaagggc
     4021 tgggtctacg agggccagcg cgtgctcccc tactgctggc aggacgagac tccgctgtcc
     4081 aaccacgagc tgcggatgga cgacgaggtc taccagcaac gccaggaccc gtcggtgacg
     4141 atctgggccg agctcgactc gggggagcgg ctgctcgcct ggacgacgac cccctggacc
     4201 ctgccgggca acctcgccat ggcggtcggc ccggacatcg actacgtcac cgtcgagcac
     4261 gaaggccagc gctacgtcct cgcggaggcg cggctcgcgt catacacgcg cgagctcggt
     4321 gaggacgcgg ccacccgagt tgtcgcgcgg gtcaagggag cccagctcgt ggggcgccgc
     4381 tacgcgccgc tcttcccgta cttctccaac gtcgacgtct ggggcactgc acaggcgttc
     4441 acggtcatcg cgagcgagga gacgacgacc gaggacggca ccggcatcgt gcacatggcg
     4501 cccgcgtacg gcgaggtcga ccagctcgag tgcgaggcca acggcatccc gacgatcctc
     4561 acggtcgacg agcgggccgt cttcacctcg gtcgtgccgg actacgcagg cctgcaggtc
     4621 ttcgaggcca acaggccgat catcaacgac ctcagggcgg ctgggcgcct catgcgcgag
     4681 gagtcctacg tccactccta cccgcactgc tggcgctgcc gcacgcccct cgtctacaag
     4741 gccgtgtcgt cgtggttcgt ccaggtgacg gcgt
//
LOCUS       DATAIX010000011         1831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_268358, whole genome shotgun sequence.
ACCESSION   DATAIX010000011 DATAIX010000000
VERSION     DATAIX010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268358"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="VHM65_00200"
     CDS             <1..1094
                     /locus_tag="VHM65_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEX2704155.1"
                     /translation="AEALCGPLSNRGLAVVSERNADQAVSAFRAAMADLLNADPRGIV
                     YGRSATSLTYDFSRHLAKAWSAGDEIVLATLDHDCNIRPWVQAAARAGVTVRWWEFDP
                     ATTELSVADLAGLLTPRTRLVAVTAASNLLGTMPPIEQISATVHEVGALLYVDGVHYA
                     AHAVVDLARLGADFFVCSPYKFMGPHCAALACAPGLLETLRPDKLAPSTDAVPERFEL
                     GTLPYEVMAGTTAAVDFIAGVAATGPAGRRERVVAGSSAFEVHELALRSAVETSLRGW
                     DRVTVHSKAVRRTPTLFFTIDGIRSWDVYLELADADILVPAGSFYADEAFRRLTLSDP
                     HGLRVGIAPYNDEHDVDRLLDALARIVAR"
     gene            1227..1805
                     /locus_tag="VHM65_00205"
     CDS             1227..1805
                     /locus_tag="VHM65_00205"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX2704156.1"
                     /translation="MTNTTSTTGTPRAGEQSDLLETLATHRHFLRFTVRDLSDEQALQ
                     RTTASALTLSALIKHVADTEEGWVDFIIGGPSAMGGAESWNDGDPDHSGGDGDGDGWP
                     EGEDPRFVIEPGTTLAGLLERYADVARRTDELVATVDLDASHPLPEAPWFEPGARWSA
                     RRVLLHIIAETAQHAGHADIIRESLDGAKSMG"
BASE COUNT          269 a          695 c          601 g          266 t
ORIGIN      
        1 tggccgaagc gctctgtggg ccgctgtcca accgaggcct ggcggtcgtg tcggagcgca
       61 acgcggacca ggcggtctcg gcctttcgcg ccgccatggc cgatctgctc aacgcggacc
      121 cgcgcggcat cgtctacggc cgcagcgcca cctcgctcac ctacgacttc tcccggcatc
      181 tggctaaggc ctggtcagcg ggtgacgaga tcgtcctcgc cacgctcgac cacgactgca
      241 acatccggcc gtgggtccag gcggcggccc gcgccggtgt caccgtccgc tggtgggagt
      301 tcgaccctgc cacgaccgag ctgtcggtcg ctgacctcgc cgggctcctc accccacgga
      361 ccaggttggt ggccgtgact gccgcgtcca acctgctggg caccatgccg ccaatcgagc
      421 agatctccgc cacggtccac gaggtcggcg ccttgctcta cgtcgatggg gtccactacg
      481 ccgcccacgc ggtggtcgac ctggcccgcc tcggcgcgga cttcttcgtg tgctccccgt
      541 acaagttcat ggggcctcac tgtgcagcgc ttgcctgcgc gcccggcctg ctcgagaccc
      601 tgcgacccga caagctggcg ccctccaccg acgccgtccc cgaacggttc gagctcggca
      661 ccctgccgta cgaggtcatg gccggcacca ccgcagccgt cgacttcatc gccggcgtcg
      721 cggcgacggg cccggccggc cggcgagaga gggtcgtcgc cggctcgtcg gcgttcgagg
      781 tgcacgagct cgccctgcgg agcgccgtcg agacctctct gcgcgggtgg gaccgggtga
      841 cggtgcactc caaagcggtc cgccggacgc cgactctgtt cttcacgatc gacggcatcc
      901 gctcctggga cgtctacctg gagctcgccg acgcggacat cctcgtgccc gccggctcgt
      961 tctacgccga cgaggcgttc aggcgcctca ccctgagcga cccccatggc ctgcgggtcg
     1021 gcatcgcccc ctacaacgac gaacacgacg tggaccggct tctcgacgcc ctggcccgga
     1081 tcgtcgcccg ctgacccacg tcctcgagcg aggccctgct gtgcacgctc gaccgggcag
     1141 ctagtattcc ggcagtcacg tcaggacgag gcacgtgagg gtccgtacgt acgtcgtgcc
     1201 ggaccaccga cggtaaggcg gacacgatga ccaacaccac gagcaccacc ggaacccccc
     1261 gggccggcga gcagagcgac ctgctggaga cactggccac gcaccggcac ttcctgcggt
     1321 tcaccgtccg cgacctcagc gacgagcagg cgctgcagcg caccaccgcc agcgccctga
     1381 ccttgagcgc cttgatcaag catgtcgcgg acaccgagga gggctgggtc gacttcatca
     1441 tcggcggtcc cagcgcgatg ggcggggccg agagctggaa cgacggtgac cccgaccact
     1501 ccggcggtga cggtgacggt gacggctggc ccgaaggcga ggacccgcgg ttcgtcatcg
     1561 agcccggaac gacgctggcc gggctgctgg agcgctacgc cgatgtggcg cgccgcaccg
     1621 acgagctggt cgcaaccgtc gatctggacg cctcgcaccc cctgccggag gcgccgtggt
     1681 tcgagccggg cgcacgctgg tcggctcgcc gggtgctcct gcacatcatc gccgagaccg
     1741 cccagcacgc cggccacgcc gacatcatcc gcgagtccct cgacggcgcc aagagcatgg
     1801 gctgacctca cctccgagtg cgctgcaacg c
//
LOCUS       DATAIX010000012         2548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_60163, whole genome shotgun sequence.
ACCESSION   DATAIX010000012 DATAIX010000000
VERSION     DATAIX010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60163"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1567)
                     /locus_tag="VHM65_00210"
     CDS             complement(47..1567)
                     /locus_tag="VHM65_00210"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF002868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX2704157.1"
                     /translation="MLLREVVEVSAVVGSTPSRLAKVAALADLLARLDPEDVEPVVGF
                     LLGRARQGRVGVGWAAVSSADRPAADVSTLTVADVDGLVTELAGISGAGSGEAVAAAL
                     GRVLARATEQEQQFVARVLLGDLRTGALEGVLTDAAARAAGVRADILRRAVMLSGDLG
                     GTVRLALAAGIGELEAVSLCVGTAVFPMLASTAVDPATALARTGAASVEYKLDGARIQ
                     VHREGEDVRIFTRNLADITHRLPELVEIVRRLPVDHVILDGETLTLDEDGDPRPFQET
                     ISRFSADAPREALLRPWFFDVLHVDGVDLIDRPLSERLVVLDRVVGEYRVPGELTDDP
                     RVAERVSVEALAAGHEGIVVKGLDSAYAAGRRGTSWLKVKPVHTYDLVVLAAEWGSGR
                     RTGWLSNLHLGARDPLGDFGEPGGYVMVGKTFKGLTDDLLRWQTERFPQLETHRSRYA
                     VHLEPRIVVEIAIDGVQRSRRYPGGIALRFARVKRYREDKPAVEADTITALRALLP"
     gene            complement(1693..2424)
                     /locus_tag="VHM65_00215"
     CDS             complement(1693..2424)
                     /locus_tag="VHM65_00215"
                     /inference="COORDINATES: protein motif:HMM:NF025068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HEX2704158.1"
                     /translation="MAAQGLTTNGRGQASRLGALAAPAAFTATTVGVYFHVIKNGTAG
                     HLSADEIESQIDVLNAAYLGTGFSFTLTATETTNNAAWYNLASGSAAERAMKTALRKG
                     TKDDLNIYTANLSGGLLGWATFPKSAFDLMDGVVVLDESLPGGSAAPYNLGDTATHEV
                     GHWVGLYHTFQGGCSSQGDRVSDTAAEKTPAFGCPTGRDTCPAKAGKDPITNFMDYTD
                     DVCMNTFSAGQVTRMQNQWVTYRAA"
BASE COUNT          367 a          920 c          904 g          357 t
ORIGIN      
        1 ctcctgcgct cactcaacgg cgaaggaaag ggcgaccctc gcccggtcag ggcaggaggg
       61 ctcgaagcgc ggtgatggtg tcggcctcga cggctggctt gtcctcgcgg tagcgcttga
      121 cccgggcgaa gcgcagggcg atcccgcccg ggtagcgtct ggagcgctgc acgccgtcga
      181 tagcgatctc gaccacgatg cgcggctcga ggtgcacggc ataccgcgat cgatgggtct
      241 cgagctgggg gaagcgctcg gtctgccagc gcagcaggtc gtcggtgagc cccttgaacg
      301 tcttgcccac catgacgtag ccgcctggtt cgccgaagtc cccgagcggg tcgcgggccc
      361 cgaggtgcag gttcgagagc cagccggtgc ggcgcccgga cccccactcg gccgcgagga
      421 cgacgaggtc gtaggtgtgc accggcttga ccttgagcca cgacgtgccc cgtcgaccgg
      481 ccgcataggc cgagtcgagc cccttgacga cgatcccttc gtgaccggcc gcgagggcct
      541 cgaccgacac tcgctcagcc accctcgggt cgtcggtcag ctcaccgggc acgcggtact
      601 cccccacgac acgatcgagc acgaccagcc gctcggacag cggccggtcg atgaggtcca
      661 cgccgtcgac gtgcagcacg tcgaagaacc agggtcgcag cagcgcctcc cggggagcgt
      721 cggcgctgaa ccggctgatc gtctcctgga acgggcgggg gtccccgtcc tcgtcgagtg
      781 tcagggtctc cccgtcgagg atgacgtggt cgaccgggag tcggcgcacg atctcgacga
      841 gctcgggcag ccggtgggtg atgtcggcga ggttgcgggt gaagatacgc acgtcctcgc
      901 cctcccgatg cacctggatc cgggccccgt cgagcttgta ctccaccgat gccgcacccg
      961 tcctggccag agccgtcgcc gggtcgaccg ccgtcgaagc gagcatcggg aacaccgccg
     1021 tgccgacgca cagggacacc gcctcgagct cgccgatgcc ggcggccagc gcgagccgga
     1081 ccgtcccacc gaggtctccc gagagcatga cggcccggcg cagtatgtcg gcccgcaccc
     1141 cggcggccct ggccgcagcg tcggtgagca cgccctcgag cgccccggtc cgcaggtcgc
     1201 cgagcagcac ccgggcgacg aactgctgct cctgctcggt tgcccgggcc agcacccgcc
     1261 cgagcgcggc cgccaccgcc tcgcccgagc ccgccccgga gattcccgcg agctcagtga
     1321 cgagcccgtc gacgtcggcc accgtgaggg tcgacacatc ggccgccggg cggtccgcgc
     1381 tcgacacagc cgcccagccg accccgacgc gcccctggcg tgctcgcccc agcaggaagc
     1441 ccaccacggg ctcgacatcc tcggggtcga gccgcgccaa gaggtcagcg agcgctgcga
     1501 ccttggccag ccgcgagggc gtcgacccga caacggccga cacctcgacg acctcgcgca
     1561 gcagcatgcc cgccagtctg acaccgcccc cggacacagc gacccgcgat gtcgccggcc
     1621 ccgtgcgagg gcccgggtca gccgactggg ccaggcgtca gcgcgggcgg tgcgtcgacg
     1681 atcgggtgcg ccctaagcag cccggtaggt cacccactgg ttctgcatcc gggtgacctg
     1741 ccccgccgag aaggtgttca tgcacacgtc gtcggtgtag tccatgaagt tcgtgatcgg
     1801 gtccttgccg gccttggccg ggcaggtgtc acgcccggtc gggcagccga acgcgggggt
     1861 cttctcggcg gcggtgtcgc tgacccggtc gccctggctg ctgcacccgc cctggaacgt
     1921 gtggtagagg cccacccagt gaccgacctc gtgcgtcgcc gtgtcaccga ggttgtatgg
     1981 cgccgcggag ccaccgggca gactctcgtc gaggaccacg acgccgtcca tgaggtcgaa
     2041 ggccgacttc gggaaggtcg cccagccgag caggcccccg gagaggttgg cggtgtagat
     2101 gttgaggtcg tccttggtgc ccttgcgcag ggcggtcttc atcgcgcgct cggccgcgga
     2161 gccactggcc aggttgtacc acgccgcgtt gttcgtcgtt tcggtggcgg tcagcgtgaa
     2221 ggagaaaccg gtcccgaggt acgcggcgtt gaggacgtcg atctggctct cgatctcgtc
     2281 ggcgctgaga tgcccggcgg tgccgttctt gatgacgtgg aagtagaccc cgacggtcgt
     2341 cgccgtgaag gcggccgggg cggcgagggc cccgagccgg gacgcctgcc cgcgcccgtt
     2401 ggtcgtcagg ccctgggcgg ccatggcctg ggccgtcgcc gattcgaggg ccgtgacctg
     2461 cgcctcggtc agctccgcgc gagccgccca gggtggcaac cgcaaggacc tcaacgagct
     2521 gaccgaggcg caggtcacgg ccctcgaa
//
LOCUS       DATAIX010000013         3608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_149547, whole genome shotgun sequence.
ACCESSION   DATAIX010000013 DATAIX010000000
VERSION     DATAIX010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149547"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VHM65_00220"
     CDS             <1..1117
                     /locus_tag="VHM65_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704159.1"
                     /translation="HQITGGEEDPLATAMLAGLAERGVTSIFLAWLTQLPGSPTVAGV
                     HAAIATTLAWAPLRHKRISRVTAEELPWWMELFGTIIGAAAPEGARTPDGFCDLTTAA
                     ILSHSTVTELAASSLLGERPDPATAANVAMLTGLLITNGPGAITAQGAKGAVSADGPQ
                     TPRRVQLNKAMIGFLSHAGYAHGGNGYEGIAFLLERFRESGLVDPGDPDHGLDLAAMT
                     HEFAADFAAATVRDKELGVARTAIPCINHPVFKGKAVNIDPREQFVRELMVQRGDYNV
                     FHEYYRELVQALFDEGATRTVFCVNVDALIATLLLKMLWPRYRDGSLREPELENAAFT
                     AFLFGRMVGCAAEIDDHQNRGRNLDTRTPPRSLSFVS"
     gene            1114..2565
                     /locus_tag="VHM65_00225"
     CDS             1114..2565
                     /locus_tag="VHM65_00225"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX2704160.1"
                     /translation="MRVCVLGAGALGSALGAMLHRGGLDVVLVHRRAEIVDHLNAEGL
                     LLRGAGADELVRVPAALVGEDLGRFDAVVVLVKSTNTRAAMESLVGSIGPSTVVLTLQ
                     NGLGNEETLGEVVGAQRVVAGRTYAGAVTLGPGVVLAGLAGRRTIIGELDGRRSDRLV
                     RLAQAFTAAGVQTEVSERIHSVMWDKLLTNVATGAVSALTGLAYGALYAAPALQATAC
                     AAVAEAMSVAAAAGIELGSSDPEEVWRGAAEGLPAGFKTSMLQSVEARSVTEVDVVNG
                     AVSRWGRRVGVATPVNDTLTALVGALPRAEVGAPAGGRSAMDRPVVPRAPEIYFEDAQ
                     VGDGCVTPAFTVTAEVIDAYARVSGDHNRVHVDEDFAQASHFGRRVAHGLFGLALVDG
                     LKSQSELRFHSGYSLGWTVDFVKPIGIGDTLRGRFWVASKRESRSRPEWGIVVLPSEL
                     LNQDDEVVQRSEHRLMVPRRPAAPVRHTDQKGA"
     gene            2566..>3608
                     /locus_tag="VHM65_00230"
     CDS             2566..>3608
                     /locus_tag="VHM65_00230"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX2704161.1"
                     /translation="MGTQPLPLAGIRVVDYTHFLAGPYLTRCLAALGADVVKVERPVS
                     GDAAREHPYLVKGYSGYFMQQNVGKRDLCVNLKDPRGLELIYRLTDSADVFVENYRPG
                     ALDRLGLGYPRLSERNPRLVYCSVSANGHTGPDAAQPGFGLIAEAKGGAMSFLGVPGQ
                     APPLFRVALADMYAGIHGVAGVCAALLGRVGSGRGQHVDISLYDCMIGLHEYAVQNYL
                     LSGGTILPEQTGHDLPQSTVYGVFTASDGYLVIAAQQDDTWTALARLIGGDDLAADRR
                     MHGLEGRNSQLPQIRQWVDAWAAGRTVVECLDALTAAGVPCAKIQTIDEVVNDPQTIA
                     RGMIIEQDHPVLGR"
BASE COUNT          536 a         1292 c         1256 g          524 t
ORIGIN      
        1 ccaccagatc accggaggtg aggaggaccc gctcgccacc gccatgctcg ccggcctggc
       61 ggagcgtggt gtgacctcga tcttcctggc gtggctcacc cagctgcccg gctcgcctac
      121 cgtggccggg gtccatgctg ccatcgcgac gacactggcc tgggcgccgc tgcgccacaa
      181 gcgcatctcc cgggtgacgg cggaggagct gccctggtgg atggagctgt tcggcacgat
      241 catcggggcc gcagccccgg agggggcacg cacgccggac ggcttctgtg acctgaccac
      301 ggcggcgatc ctgagccact cgacggtcac cgagctcgcc gcgtcgagcc tgctcggcga
      361 gcggccagac cccgcaaccg cagccaacgt cgccatgctc accggcctgc tcatcaccaa
      421 cggcccaggg gcgatcacgg cccagggcgc caagggtgcg gtctcggccg acgggcctca
      481 gacgcccagg cgcgtccagc tcaacaaggc catgatcggc ttcctcagcc acgccggcta
      541 cgcgcacggt ggcaacggct acgagggcat cgccttcctg ctcgagcggt tccgcgagag
      601 cgggctcgtc gacccggggg acccggacca tggcctcgat ctcgcggcca tgacgcacga
      661 gttcgccgcg gacttcgcgg ctgccacggt ccgggacaag gagctggggg tcgcgcgcac
      721 cgccataccg tgcataaacc acccggtctt caagggcaag gcggtgaaca tcgacccccg
      781 ggagcagttc gtccgagagc tcatggtgca gcgcggcgac tacaacgtct tccacgagta
      841 ctaccgcgag ctcgtccagg cgctgttcga cgaaggagcc acgcggaccg tcttctgcgt
      901 caatgtcgac gccctcatcg ccaccctcct gctcaagatg ctctggcccc gctaccgcga
      961 cggaagcctg cgcgagccgg agctggagaa tgccgccttc acggccttcc tcttcggccg
     1021 catggtcggc tgcgccgccg agatcgacga ccaccagaac cgcggacgta acctggacac
     1081 gcgcacccca ccgcgctccc tgagcttcgt gtcatgaggg tctgcgtgct cggcgcgggg
     1141 gccctcgggt cggcccttgg cgcgatgttg caccgaggcg gcctcgatgt cgtgctcgtg
     1201 caccgccggg cagagatcgt cgaccacctc aacgccgaag ggctgctgct gcgcggcgcc
     1261 ggcgcggacg agctggtccg ggtgcccgcc gccctcgtgg gcgaggacct cggtcggttc
     1321 gatgccgtcg tcgtcctggt caagtcgacc aacacgcgcg ccgccatgga aagcctcgtg
     1381 gggtcgatcg gcccgtcgac cgtggtcctg acgctgcaga acgggctcgg caacgaggag
     1441 accctcggtg aggtcgtcgg ggcccagaga gtggtcgcgg gacggacgta tgccggtgcc
     1501 gtcacccttg ggcctggggt ggttctggcc ggcctcgccg gtaggcggac gatcatcggc
     1561 gagctcgacg gtcggcgcag cgatcggttg gtgcgcctcg cgcaggcctt caccgcggcc
     1621 ggtgtgcaga ccgaggtgag cgagcggatc cactcggtca tgtgggacaa gctcctcacc
     1681 aacgtcgcca ctggtgccgt cagcgcgctg accggcctgg cctacggcgc gctctatgcc
     1741 gcaccagccc tccaggcgac ggcgtgcgcc gcggtcgccg aagcgatgtc ggtggccgcc
     1801 gcagccggga tcgagctcgg ttcgagcgac cccgaagagg tatggcgagg cgccgccgag
     1861 gggctcccgg cgggcttcaa gacctcgatg ctgcagagcg tcgaggcccg ctcggtcact
     1921 gaggtcgatg tcgtcaatgg tgccgtgagc cgatggggac gacgcgtcgg ggtcgcgacg
     1981 ccggtcaacg acacgctgac ggccctggtc ggggcgctgc cccgtgctga ggtcggcgcc
     2041 ccagcagggg ggcggtccgc gatggaccgg cccgtcgtgc cgcgggcacc cgagatctac
     2101 ttcgaggacg cccaggtcgg tgacgggtgt gtcaccccgg ccttcacggt gacggccgag
     2161 gtcatcgacg cctacgcgcg cgtgtcgggt gaccacaacc gggtccatgt cgacgaggac
     2221 ttcgcccagg cgagccactt cgggcgccgg gtcgcccacg gcctgttcgg gctggccctg
     2281 gtcgacggcc tcaagagcca gagcgagctg cgcttccact ccggctactc actgggctgg
     2341 accgtcgact tcgtcaagcc gatcggcatc ggagacaccc ttcgcgggcg gttctgggtc
     2401 gccagcaaac gcgagtcccg cagccgtccc gagtggggga tcgtcgtgct ccccagcgag
     2461 ctgctcaacc aggacgacga ggtcgtccag cgaagcgagc accgcctcat ggttccgcgc
     2521 cgcccagccg cgccggtccg tcataccgac cagaaaggcg cctagatggg cacccagccg
     2581 ctgcccctcg cgggcatccg cgtagtcgac tacacccact tcctggccgg gccctacctg
     2641 acccggtgcc tggcggccct gggcgctgac gtggtcaagg tggagcggcc ggtgtcggga
     2701 gacgccgccc gcgagcaccc atacctcgtc aagggctaca gcggctactt catgcagcag
     2761 aacgtcggca agcgtgacct gtgcgtcaac ctcaaggacc cgcgcggcct ggagctgatc
     2821 taccggctca ccgactccgc cgatgtgttc gtcgagaact accggcccgg ggcgctggac
     2881 cggctcgggc tcggctaccc tcggctgtcc gagcgcaacc ccaggctggt ctactgctcg
     2941 gtgtcggcca acgggcacac cgggcccgac gcggcccaac cggggttcgg cctgattgcc
     3001 gaggccaagg gcggtgccat gtccttcctt ggggtgcccg ggcaggcccc gccgctgttc
     3061 cgggtcgcgc tggccgacat gtacgcgggg atccacgggg tggccggcgt gtgtgcggcg
     3121 ctcctcggcc gggttggcag cggccggggc cagcacgtcg acatctcgct ctacgactgc
     3181 atgatcggcc tgcacgagta tgccgtccag aactacctgc tcagcggggg gaccatcctg
     3241 cccgagcaga ccgggcacga cctgccgcag tcgacggtgt acggggtgtt caccgccagc
     3301 gacggctacc tcgtcatcgc ggcccagcag gacgacacgt ggacggcgct ggcgcggctc
     3361 atcggcggcg acgacctggc cgccgaccgg aggatgcacg ggctcgaagg acgcaacagc
     3421 cagctgcccc agatccgcca gtgggtcgat gcctgggccg ccggacgcac cgtcgtcgag
     3481 tgtctcgacg cgctcaccgc ggccggcgtg ccgtgcgcca agatccagac gatcgacgag
     3541 gtcgtcaacg acccgcagac catcgcccgc gggatgatca tcgagcagga ccacccggtc
     3601 ctcggccg
//
LOCUS       DATAIX010000014         5917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_30467, whole genome shotgun sequence.
ACCESSION   DATAIX010000014 DATAIX010000000
VERSION     DATAIX010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30467"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VHM65_00235"
     CDS             complement(<1..378)
                     /locus_tag="VHM65_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704162.1"
                     /translation="MALVLTNGLVTTATESRRADVLVVDGRIGDVGDLTPGPGDEVLD
                     CTGCLVLPGGVESHCHLDLECGPGLVTADDFASGTRSALAGGTTTVIDFATAFHGQSL
                     AEGLRRWHAKAEGRAVADYAFHLA"
     gene            complement(396..1718)
                     /gene="ssnA"
                     /locus_tag="VHM65_00240"
     CDS             complement(396..1718)
                     /gene="ssnA"
                     /locus_tag="VHM65_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HEX2704163.1"
                     /translation="MIVGNGPVITNDPAKPFVEAGAVHVVGTVVAAVGPAAELRDAHP
                     GEPFVDVGGRVIMPGLINAHTHAYSAYARGMAVHLPTRDFTEILENLWWRLDRLLTME
                     DVDLNTATTFIDCIRNGVTTVFDHHSSPDAVTGALHRQAEVARRLGIRASLCYETSDR
                     DGPDVLARAIQENVSFMNEANTSDQDMVKGLFGLHASFTLSQESMEACLEAKDGVPGG
                     FHVHTAEGPADEPDSEGKYGKRIVERFHHLGLLGPQTLAVHCVYANEGELDIIKDTDT
                     SMVHNPHSNMGNAVGGAPVVEALAKGVRCGLGTDAYTADMFASASVAKILASHRLGDP
                     TVGFGHALDLLLGNNPKIAAKHFAKPLGVLAPGAYADLITLTYDPITPLNAGSAGGHI
                     LFGMNGSMVNDTMVNGTWVMRDRVIVTLDEAEVNARSREAAPRIWARM"
     gene            complement(1715..4759)
                     /gene="ygfK"
                     /locus_tag="VHM65_00245"
     CDS             complement(1715..4759)
                     /gene="ygfK"
                     /locus_tag="VHM65_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HEX2704164.1"
                     /translation="MGDLMRPLSFDHLMTWATTELRQDGSIFGVRRDQFWRPEPGRLV
                     TDPFGERLAVPVGPAAGPQTQLANNILVAYLSGARFMELKTVQKMDGEQIRQAVAKPC
                     IEAEDEGYNCEWSTELTVQEALDEYVKAYFAIAVFAREMQLGSIDEVAFNISVGYDLE
                     GIKGAKVNPFVDAMMDASATPIYQECSAWVESHLTGFAHFDRSDLEGLSPHLSHSVTL
                     STLHGCPADEIERIASYLMRDKHLNTFVKCNPTMLGYDTARAIMDELGYEYVSFDDHH
                     FTADLQYGDAIAMFRRLMALARELGLTFGVKLTNTFPVEVTRGELPADEMYMSGRSLL
                     PLTISLAAKLSREFAGTLPISFSGGVDAFNVAELLETGIQPLTAATTILKPGGVIRFN
                     QMAVSAAAVMTDYRGIDVERLDRLVADVFSDERYHKRYREKVHSRKTASPLPLTDCYK
                     APCEHGGCPIEQRIPQYLTLTAAGRFAEAFDVIEVDNTAPTITGVLCPQPCRDHCTRL
                     DYDSSIDMRAVKLAAADGAQDAFMAATTPSPLRTDHKVAVIGAGPAGIAAAMFLRRNG
                     VDVDVFEKQPGPYGIVKYIIPAFRISREQIERDYQLAVAMGVRFHFDCDPGYSVSALG
                     QTYGHVVIATGSWGHGQNPVREGRDKIVDALDFLWQANNEGGPIVGKRVAVIGAGDVA
                     MDCVRTATRAPGVEEAVIVYRRTEPNMPATQHEVNLVRGEGITMHELYGPISYDGRTL
                     HCEEMALRDVDASGRRSVTGRGTFVDLPFDTVIGATGATIDTTAYAANGLGLDLRGRV
                     VLDESYQSSVEGVYVIGDGRLGPDTIVRAIADAKVVARAILAAHNLSVDFDRLTPVTH
                     RPESEVHGRRGLMIASINGQAEGGRCLTCEDICEICTEVCPNRANVAITVPGFADPRQ
                     IVHIDGLCNECGNCGTFCPHAGLPYRDKVTVFWTREDFDLSIDAGFLPLPGGGYLTRT
                     PEREVFELGADRSRLSTDMARILEALELRYTYLLAAPLGVSS"
     gene            complement(4913..>5917)
                     /locus_tag="VHM65_00250"
     CDS             complement(4913..>5917)
                     /locus_tag="VHM65_00250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2704165.1"
                     /translation="RVGMQIHKIAWSDEATLRQFYEILNASQSHGRVHFVGRPFEEAL
                     SEWRQDGVDDQTALVGLWAKGKLAGVAHAWLPPEGSGGAVSAQVDVHPRWRGLGYGHQ
                     LFDALVDLGRQRGATLILAEAKYPAAQGETHPYRLFLEGIGMELTTAQLILELTLPVA
                     PGHLDRLETQAKGEFGEQYRISTWGAIPDDILPSLCELMSFVEAEAPAGRGWHADGVS
                     PESYLSEIAHNALSGRRRLTSLATELSTGAVVGFTEMLTQDGITRAHQWGTYVHPDHR
                     GHQLGMAVKAASARRLQEVRTDCVGVLTSGQTTPGMAAVNARLGFVPAEVRAAFVRTL
                     "
BASE COUNT          880 a         1980 c         2111 g          946 t
ORIGIN      
        1 ggccaggtgg aaggcgtagt cggcgaccgc gcggccctcg gccttcgcgt gccatcggcg
       61 cagaccctcg gcgaggctct ggccgtggaa cgccgtggcg aagtcgatga ccgtggtggt
      121 gccgccggcc agggccgagc gagtaccgga ggcgaagtcg tcggcggtca ccaggccggg
      181 tccgcattcg aggtccaggt ggcagtggga ctcgacgccg ccagggagca cgagacagcc
      241 ggtgcagtcg agcacctcgt cgccgggccc tggcgtgagg tcgccgacat cgccgatccg
      301 gccgtcgacg accagcacgt cggcacgccg cgactcggtc gcagtggtca ccagaccgtt
      361 cgtcaggacc agcgccacgg cgtgctcacc cttctctaca tcctcgccca gatccggggg
      421 gccgcctcgc gcgaccgcgc gttgacctcg gcctcgtcga gggtgacgat gacccggtcg
      481 cgcatcaccc aggtgccgtt gaccatggtg tcgttgacca tcgacccgtt catcccgaag
      541 aggatgtgac cgcccgcgga gccggcattc agcggggtga tggggtcgta ggtgagggtg
      601 atgaggtcgg catacgcccc gggggcgagc accccgagcg gcttggcgaa gtgcttcgcg
      661 gcgatcttgg ggttgttgcc caggagcagg tcgagggcgt ggccgaagcc gaccgtgggg
      721 tcaccgagcc ggtggcttgc caggatcttc gccacggatg cggaggcgaa catgtccgcc
      781 gtgtaggcgt cggtgccgag accgcaccgc acacccttgg ccagtgcctc gacgactggg
      841 gcgccgccga cggcgttgcc catgttggag tgcgggttgt ggaccatcga ggtgtccgtg
      901 tccttgatga tgtccagctc gccctcgttg gcgtagacgc agtggaccgc gagggtctgc
      961 ggacccagta gaccgaggtg gtggaagcgt tcgacgatcc gcttgccgta cttgccttcc
     1021 gagtcgggct cgtcggcggg gccctcggcg gtgtgcacgt ggaacccgcc cggaacgccg
     1081 tccttggcct cgaggcacgc ctccatcgac tcctgtgaca gcgtgaacga ggcgtgcagc
     1141 ccgaacaggc ccttgaccat gtcctggtct gaggtgttgg cctcgttcat gaaggagacg
     1201 ttctcctgga tggcgcgggc caggacgtcc ggcccgtccc ggtcggaggt ctcgtagcac
     1261 aggctggccc ggatgccgag gcggcgcgcc acctccgcct gccggtgcag cgccccggtc
     1321 accgcatcgg ggctggagtg gtggtcgaac accgtcgtca ccccgttgcg gatgcagtcg
     1381 atgaacgtcg tcgccgtgtt gagatcgacg tcctccatgg tgagcagccg gtcgaggcgc
     1441 caccagaggt tctccaggat ctcggtgaag tcgcgcgtcg gcaggtggac cgccataccc
     1501 cgggcatagg cgctgtaggc gtgcgtgtgc gcgttgatga gcccgggcat gatgacccgc
     1561 ccgccgacgt cgacgaacgg ctcgccgggg tgggcgtccc gcagctcggc ggccggcccg
     1621 accgcggcga ccacggtgcc gacgacgtgg accgcaccgg cctcgacgaa gggtttcgcc
     1681 gggtcgttgg tgatgaccgg gccgttgccg acgatcatga gctcactccc agcggcgcgg
     1741 cgagcaggta ggtgtaacga agctcgagcg cctcgaggat ccgcgccatg tccgtgctca
     1801 gccggcttcg gtcggcgccg agctcgaaga cctcccgctc cggcgtccgg gtgaggtacc
     1861 cgccgcccgg cagggggagg aacccggcgt cgatggacag gtcgaagtcc tcgcgcgtcc
     1921 agaacaccgt gaccttgtca cggtagggca ggccggcatg cgggcagaac gtcccgcagt
     1981 tcccgcattc gttgcacagc ccgtcgatat ggacgatctg gcgggggtcg gcgaagcccg
     2041 ggaccgtgat ggcgacgttg gcccggttgg ggcagacctc ggtgcagatc tcgcagatgt
     2101 cctcgcaggt gaggcatcgg ccaccctcgg cctggccgtt gatcgaggcg atcatcaggc
     2161 cacggcgacc gtggacctcg ctctccggac ggtgcgtgac cggcgtgagc cggtcgaagt
     2221 ccaccgagag gttgtgtgcc gccaggatcg cgcgggcgac gaccttggcg tcggcgatgg
     2281 cccgcacgat ggtgtccggc ccgagccggc cgtctccgat gacgtagacg ccctcgaccg
     2341 aggactggta cgactcgtcg aggacgaccc ggccccgtag gtcgagcccg aggccgttgg
     2401 cggcgtatgc cgtggtgtcg atcgtggccc cggtggcacc gatgacggtg tcgaagggca
     2461 ggtcgacgaa ggtgccccgg cccgtgaccg agcgccgtcc gctggcgtcg acgtcgcgca
     2521 gggccatctc ctcgcagtgc agcgtccggc cgtcatacga gatcggcccg tagagctcgt
     2581 gcatcgtgat gccctcgccc cgcacgaggt tgacctcatg ctgcgtcgcg ggcatgttcg
     2641 gctcggtccg gcggtagacg atgaccgcct cctcgacgcc gggcgcgcgc gtcgcggtcc
     2701 ggacgcagtc catcgcgacg tccccggcgc cgatgacggc gacccgcttg cccacgatgg
     2761 ggccgccctc gttgttggcc tgccacagga agtcgagcgc gtcgacgatc ttgtcccggc
     2821 cctcccggac gggattctgg ccatgccccc acgaacccgt cgcgatgacg acgtggccgt
     2881 aggtctgccc cagggccgag accgagtagc cggggtcgca gtcgaagtgg aacctcaccc
     2941 ccatcgccac cgcgagctgg tagtcccgct cgatctgctc tcgcgagatc cggaaggccg
     3001 ggatgatgta cttcacgatc ccgtaggggc cgggctgctt ctcgaagacg tcgacatcca
     3061 cgccgttgcg ccgcaggaac atcgccgcgg cgatcccggc ggggccggcg ccgatgacgg
     3121 cgaccttgtg gtcggtacgc agcggcgacg gcgtggtcgc ggccatgaag gcgtcctgag
     3181 caccgtcagc cgccgcgagc ttgaccgcgc gcatatcgat cgagctgtcg tagtccaggc
     3241 gcgtgcagtg gtcgcggcac ggctgcgggc acagcacccc ggtgatcgtc ggggcggtgt
     3301 tgtccacctc gatgacgtcg aaggcctcgg cgaaccggcc cgctgcggtg agggtgaggt
     3361 actgcgggat ccgctgctcg atcgggcagc cgccgtgctc gcacggagcc ttgtaacagt
     3421 cggtgagcgg cagcggtgaa gcagtcttgc gggagtggac cttctcgcgg tagcgcttgt
     3481 ggtagcgctc gtcggagaag acgtccgcga ccagccggtc gagccgctcg acgtcgatcc
     3541 cgcggtagtc ggtcatgacc gcggccgccg acaccgccat ctggttgaac cggatcaccc
     3601 caccgggttt gaggatcgtc gtggccgccg tcagcggctg gatccccgtc tccaggagct
     3661 cggcgacgtt gaacgcgtcg acccctccgg agaacgagat cggcagggtg cccgcgaact
     3721 cgcgggacag cttcgccgcc agcgagatcg tgagcggcag cagcgaccgg cccgacatgt
     3781 acatctcgtc ggcgggcagc tcgccccgcg tgacctcgac gggaaaggtg ttcgtcagct
     3841 tgacgccgaa ggtcaggccg agctcgcggg cgagcgccat gagccgacgg aacatcgcga
     3901 tggcgtcgcc atactgcagg tcagcggtga agtggtggtc gtcgaaggac acgtactcgt
     3961 agccgagctc gtccatgatg gcccgcgccg tgtcgtagcc gagcatcgtc gggttgcact
     4021 tgacgaaggt gttcaggtgc ttgtccctca tgaggtatga cgcgattcgc tcgatctcgt
     4081 cggcgggaca tccgtgcagc gtcgacaagg tgaccgagtg gctgaggtgg gggctcagcc
     4141 cctcgaggtc cgagcggtcg aagtgggcga aaccggttag gtgcgactcg acccaggcgg
     4201 agcactcctg gtagatcggc gttgcggagg cgtccatcat cgcgtcgacg aaggggttga
     4261 ccttggcgcc cttgatgccc tccaggtcgt agccgacgct gatgttgaag gcgacctcgt
     4321 cgatcgagcc cagctgcatc tcccgggcga acaccgcgat cgcgaagtag gccttgacgt
     4381 actcgtccaa ggcctcctgg acggtgagct cggtggacca ctcgcagttg tagccctcgt
     4441 cctccgcctc gatgcacggt ttggccacgg cctggcggat ctgttcgccg tccatcttct
     4501 ggaccgtctt gagctccatg aagcgcgcgc cggacagata ggcgaccagg atgttgttgg
     4561 ccagctgggt ctgcggaccg gccgccgggc cgaccggcac ggcgagacgc tcgccgaagg
     4621 gatccgtgac gaggcggccc ggctccgggc gccagaactg gtcccggcgc accccgaaga
     4681 tcgacccgtc ctgccgcagc tctgtggtgg cccacgtcat gaggtggtcg aaggacaacg
     4741 gccgcatgag gtcgcccatg aatgaacctc tcgtctggcc ccctcgggga accccagtct
     4801 gccaccaccc tcgggccgtc cacatctgtg cgggatcggc tcgtccccgt ccggcccacg
     4861 ctcaggctcg gacctcaccg gactacggca tcacccaggt gacgggcctg cttcagaggg
     4921 tccgcacgaa ggccgcgcgg acctcggcgg gcacgaagcc gaggcgcgcg ttgaccgccg
     4981 ccatgcccgg ggtggtctgg cctgaggtga gcacgcccac gcagtcggtc cgcacctcct
     5041 gcagtcgacg agcgctggcg gccttgaccg ccataccgag ctggtgtccg cggtggtccg
     5101 gatggacgta ggtgccccac tggtgggccc gggtgatgcc gtcctgcgtg agcatctcgg
     5161 tgaagcccac cacggccccg gtgctcagct cggtcgcgag cgaggtgagc cggcgccggc
     5221 ccgacaacgc gttgtgggcg atctcggaca ggtagctctc cggcgacacc ccgtccgcgt
     5281 gccagccgcg accggccggt gcctcggcct ccacgaagga catgagctcg cacagcgagg
     5341 gcaggatgtc atcgggtatg gcgccccacg tcgagattcg gtactgctcg ccaaactccc
     5401 ccttggcctg cgtctcgagc cggtcgaggt gtccgggcgc cacgggcagc gtcagctcga
     5461 ggatgagctg tgccgtggtg agctccatcc cgatgccctc gaggaacagg cggtaggggt
     5521 gggtctcgcc ctgggcggcc gggtacttcg cctcggcgag gatcagggtg gccccgcgct
     5581 ggcgtcccag gtcgacgagg gcgtcgaaca gctggtgtcc atagccaaga ccccgccagc
     5641 gggggtggac gtcgacctgg gcgctgaccg cgcccccaga tccctccggc ggcagccaag
     5701 cgtgggcgac ccccgcgagc ttccccttgg cccagagacc aaccaacgcg gtctggtcat
     5761 cgacgccgtc ctgacgccac tccgagagtg cttcctcgaa ggggcgcccc acgaagtgca
     5821 cccgcccatg gctctgcgag gcgttgagga tctcgtagaa ctggcgcaac gtcgcctcgt
     5881 cggaccaggc gatcttgtgg atctgcatac cgacacg
//
LOCUS       DATAIX010000015         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_208828, whole genome shotgun sequence.
ACCESSION   DATAIX010000015 DATAIX010000000
VERSION     DATAIX010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208828"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VHM65_00255"
     CDS             complement(<1..338)
                     /locus_tag="VHM65_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX2704166.1"
                     /translation="MLEALASLYPPGAADDWDRVGLVTGDPADVVRRIHLAVDPTLAV
                     IGEAVAAGADLLVTHHPLLLRGVHAVPMTTAKGASVTALIRGKVALYVAHTNADKADP
                     GVCDALAAACG"
     gene            complement(409..481)
                     /locus_tag="VHM65_00260"
     tRNA            complement(409..481)
                     /locus_tag="VHM65_00260"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(446..448),aa:Val,seq:tac)
     gene            833..1285
                     /locus_tag="VHM65_00265"
     CDS             833..1285
                     /locus_tag="VHM65_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704167.1"
                     /translation="MTETFDDPVAFAPERRIVSFQGVRFWSKDPSPPYWTVGDAEGAT
                     LYRKFSDGADTGDLAVSFVKDGDVRSFGFLLNPFGHPNQIAIRVVEVDGTVTLVLLPQ
                     NAGATYFGLHSRIGIEKVVIGQRPDLAGGWTNFSLDRVSRGHIMSGHH"
     gene            complement(1295..1705)
                     /locus_tag="VHM65_00270"
     CDS             complement(1295..1705)
                     /locus_tag="VHM65_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX2704168.1"
                     /translation="MASPSTPLSVGDTAPGFTLKNQHGESVSLADFAGAKNVVLVFYP
                     FAFSGICTGELTEIRDTLEDFQGEDLQVLAISCDPMYSLRAYADANGYFFPLLADFWP
                     HGSVAASYGVLNDLTGGALRGTFLVDRGGVIRPE"
     gene            complement(1767..>2065)
                     /locus_tag="VHM65_00275"
     CDS             complement(1767..>2065)
                     /locus_tag="VHM65_00275"
                     /inference="COORDINATES: protein motif:HMM:NF022698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3052 family protein"
                     /protein_id="HEX2704169.1"
                     /translation="LTTTVLEDEDYGDVADAVLIWWRDGDGDLVDILVESKPNLVERG
                     FLVLVTPKAGRSGYVDASEVEDAAKITGLHAAGTVSACPDWSATRLVSPRSARH"
BASE COUNT          321 a          670 c          755 g          319 t
ORIGIN      
        1 ccgcaggccg ccgccagggc gtcgcagacc cccgggtcgg ccttgtcggc gttggtgtgc
       61 gcgacgtaga gggcgacctt gccgcggatc agcgcggtga ccgacgcgcc cttggcggtc
      121 gtcatcggga cggcatggac cccgcgcagg agcagcgggt ggtgggtgac gagcaggtcc
      181 gcccccgccg ccacggcctc gccgatgacg gcgagggtgg ggtcgaccgc gagatggatc
      241 cggcgcacga cgtcggcggg gtctccggtg acgagcccga cccggtccca gtcgtcggcc
      301 gcgccgggtg ggtagagact cgcgagcgcc tcgagcacct cggcgagcgt cggagcaccg
      361 gtcggcggtt ccgtcggtgc gggtgggtcg ctgacgctct ggctcatgtg ggcccggccg
      421 ggctcgaacc gacgacaccc gcggtgtaaa cgcggtgctc taccaactga gctacaggcc
      481 cgggacatgt ctgcatcgca tcgaaacgcc atgtccggcg gttcattgtg ccaggcgccc
      541 gtgaggcatg catggtgtgt cacctgtcgt gcgctcccca ggggaacaac gactctcacc
      601 cgttcggaag gagcctgcct taggctggct cagccaggga acctcacgag ctgaggatga
      661 ctgccatgac tgggatgaga cgcgggctgg tgcgcgcgct ggtggtgctg gtgggggcga
      721 tggctccggt ctcggctggc gcagtggcca gcccggcgag cgcctcaggt cacagtggcg
      781 ggctcaccgt gactacaacg agagtgactt cctggcggcc aacacgatcc gggtgactga
      841 gacgttcgac gatccggtcg ccttcgctcc ggaacggcgg atcgtgagct tccagggtgt
      901 gaggttctgg tccaaggacc cgagcccccc gtactggacg gtgggggacg ccgaaggcgc
      961 cactctgtac cgcaagttca gcgacggggc ggacacaggg gatcttgcgg tgagcttcgt
     1021 caaggacggc gacgtccgtt ccttcggctt cctgctgaac cccttcggcc acccgaacca
     1081 gatcgctatc cgggtggttg aggtcgacgg aacggtgacg ctggtcttgc taccgcagaa
     1141 cgccggcgcc acgtacttcg ggctgcacag ccgaatcggt atcgagaagg tggtgatcgg
     1201 gcagcgtccc gacctggcag ggggctggac gaacttctcg ctcgaccggg tctctcgtgg
     1261 ccacatcatg tctgggcacc actgaaccga gctgtcactc ggggcggatg acgcccccgc
     1321 ggtcgacgag gaacgtcccg cgcagcgcgc cgccggtgag gtcgttgagc acgccgtagg
     1381 acgcggcgac gctcccgtgc ggccagaagt cggcgagcag cgggaagaag tagccgttgg
     1441 cgtcggcata cgcccgaagg gagtacatcg ggtcgcacga gatggccagc acctgcaggt
     1501 cctcgccctg gaagtcctcg agggtgtcgc ggatctcggt cagctcgccc gtgcagatcc
     1561 ccgagaacgc gaacgggtag aagacgagca cgacgttctt ggcgcccgcg aagtccgcca
     1621 ggctcaccga ctccccgtgc tggttcttca gggtgaagcc gggcgcggtg tcgccgacgg
     1681 acaagggggt cgaggggctg gccatggccc cagtctgcca gggcgcccgg accgcgtgcc
     1741 cgaccgttcc gctgcctgaa ctcgactcag tggcgggccg accgaggcga cacgagccgg
     1801 gtggcgctcc agtcggggca ggcgctgacc gtgccggcgg cgtgcaggcc ggtgatcttg
     1861 gccgcgtcct cgacctcgct ggcatcgaca tacccggaac ggccggcctt gggcgtcacc
     1921 aggacgagaa acccgcgctc gacgaggttg ggcttggact cgacgaggat gtcgacgagg
     1981 tcaccatcgc cgtcgcgcca ccagatgagg accgcatcgg cgacatcgcc gtagtcctcg
     2041 tcctcgagca cggtcgtcgt caggt
//
LOCUS       DATAIX010000016         1898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_60347, whole genome shotgun sequence.
ACCESSION   DATAIX010000016 DATAIX010000000
VERSION     DATAIX010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60347"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VHM65_00280"
     CDS             complement(<1..466)
                     /locus_tag="VHM65_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704170.1"
                     /translation="MSDLKPLYPVSRRHLEALTDATGIMQHALGRRPNPEHGYCTDDV
                     SRSLTVDLLHGRELGWAAVSPSAWRSYRFLVDAFESSTGRFRNFRAIDGTWLDSPGSE
                     DAHARAVLALAESMVAAPEPGFREAASDLFARALPATLELRALRPRSSALLAC"
     gene            complement(459..1631)
                     /locus_tag="VHM65_00285"
     CDS             complement(459..1631)
                     /locus_tag="VHM65_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2704171.1"
                     /translation="MSIRTAFVSTYPPRRCGIASFTRDLASVVGAHEVVALQSTDRVE
                     RYPPEVHHRIRRDVDADYARAARALRDCDVDVVSIQHEYGIWGGEDGASVLDFVRALD
                     KPVVATLHTVLRHPTPSQRRILSQLVRGATTTVVMSGSAASLLTTVYDVGSSQLEIVP
                     HGVPDLDLVDSSAAKPSLGLAGRRVLLSFGLLGPSKGFELAIEALPAIVRDVPDVCYV
                     VLGATHPDLLRRDGESYRRSLVERTAALGMADHVRFVDRFVSPGELGRWLGAADVVVT
                     PYPNLDQIVSGTLSYAMGAGRAVVSTPYAYAAELLADGRGVLVQPGSPGALADAVANL
                     LRDPALRATVGRRAYEYSRPMVWQAVGAAYARVFARVARAAPGPAIASVPMPPIRV"
BASE COUNT          328 a          652 c          672 g          246 t
ORIGIN      
        1 cgcatgccag cagggccgat gaccggggac gcaacgcccg cagctcgagc gtcgccggga
       61 gtgcccgcgc gaacaggtcg gaggcagcct cgcgaaaccc gggctcaggc gcagccacca
      121 ttgactcggc gagcgccagc acggcccgcg cgtgtgcgtc ctcggacccg ggcgagtcga
      181 gccaggtccc gtcgatggcc cggaagttcc gaaagcggcc ggttgagctc tcgaacgcat
      241 ccacgaggaa ccggtatgag cgccacgcgc tgggactgac ggcggcccag ccgagctcgc
      301 gcccatggag caggtcgaca gtcagggagc gggacacgtc gtctgtgcag tacccatgct
      361 ccggattcgg ccgacgtccc agcgcatgct gcatgatccc ggtcgcgtcg gtgagcgcct
      421 cgagatgccg ccgactcacc gggtacagcg gcttgaggtc agacacggat cgggggcatc
      481 gggactgagg cgatcgcggg accaggcgcc gcgcgcgcaa ctcgcgcgaa cacgcgcgcg
      541 tatgccgccc cgaccgcctg ccagaccatc gggcggctgt actcgtaggc ccgacggccc
      601 acggtcgcgc gcaaagccgg atcccgcagg aggttggcaa cggcatcggc gagcgccccg
      661 ggtgagcccg gctggacgag cacgccgcgt ccgtcggcga gcagctcggc cgcataggca
      721 tacggcgtcg acaccacggc cctgccagcc cccatggcgt aggacagcgt gccggacacg
      781 atctggtcga ggttcgggta cggcgtgacc acgacgtccg ctgccccgag ccagcgcccg
      841 agctcgcccg ggctcacgaa gcggtcgaca aaccgcacgt ggtcggccat cccgagcgcg
      901 gccgtccgct cgacgaggct ccgccgatag gactccccgt cgcgtcggag cagatctggg
      961 tgcgtcgctc caaggaccac gtagcacacg tcgggcacgt cgcgcacgat ggcgggcagg
     1021 gcctcgatcg ccagttcgaa gcctttgctc gggcccagca gcccgaagct cagcaggacc
     1081 cgccgcccgg caaggcccag ggacggtttg gcggcgctcg agtcgacgag gtccaggtcc
     1141 gggacgccat ggggcacgat ctccagctgg ctcgagccga cgtcatagac cgtggtcagg
     1201 agtgacgcgg cggaccccga catcaccacc gtcgtcgtcg cccctcgcac cagttgcgag
     1261 aggatccgtc gctgggatgg ggtcgggtgt cgcaggaccg tgtggagcgt ggcgacgacc
     1321 ggtttgtcga gggcgcggac gaagtcgagg acgctggcgc catcttcgcc gccccagatg
     1381 ccgtactcgt gctgaatgga gacgacatcc acgtcgcagt cacggagcgc gcgggccgcc
     1441 cgcgcgtagt cggcgtccac atcccgccgg atgcgatggt ggacttcggg cgggtaccgc
     1501 tccacccggt cggttgactg cagcgcgaca acctcgtgcg ccccgacgac agaggcgagg
     1561 tcacgggtaa acgatgcgat cccgcaacgg cggggtggat aggtcgagac gaaggccgtc
     1621 cgaatactca tgccgcgctc ctctcgggac gcagatgccg agccggacga acagggagtt
     1681 cgactggacg acgcgtgcgg attccggtga cctggccata cgctcgactg accggcaagc
     1741 gtgtctggct gacaccgtat accgccggag cacgacggtg accgacctcg cgccccctgg
     1801 gagaggggat cgacaccatg cgcgacgact ccaaagtggg gctcgcccga gaccctgtca
     1861 agcaacaagt gaccgttgcc atgcgatcag gttgggaa
//
LOCUS       DATAIX010000017         1527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_268488, whole genome shotgun sequence.
ACCESSION   DATAIX010000017 DATAIX010000000
VERSION     DATAIX010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268488"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VHM65_00290"
     CDS             <1..575
                     /locus_tag="VHM65_00290"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX2704172.1"
                     /translation="LARRINASGLRLRKHKGEQGIGRFVGVPIDGGATADIDLVASTS
                     PSPEAVTGAAAVLASDLEGSVLLVHSVRRGTWDCPGGRREEGERVVDCARREVLEETG
                     LGLEESELGYAGYERLTVTDPGHWAYPSPFVQVFQARLGLLRPQLAVGADDVDGARWV
                     SERELDDLCSRLFWWPLARHLLSREPRRSR"
     gene            complement(547..1218)
                     /locus_tag="VHM65_00295"
     CDS             complement(547..1218)
                     /locus_tag="VHM65_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704173.1"
                     /translation="MHPLQLAWLGDCAVLCPQGSADVARDLGDELLDRWSEPARAYHC
                     TTHLTEVLAALTELTSAGLLRGLDVSLARMAAWFHDAVYDPRAGAGANEAASAALARH
                     TLGRLNAAPHHVERVVDLVEMTVDHDPQGIDPVTDAFHDADLWILASPPARYDEYTLQ
                     IRAEYAQVSALEFARGRRAVLATFLARDPIYRTPHAQAAWDPQARVNLARSVAVLSEI
                     VGVRG"
     gene            complement(1228..>1527)
                     /locus_tag="VHM65_00300"
     CDS             complement(1228..>1527)
                     /locus_tag="VHM65_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX2704174.1"
                     /translation="AQHAYLGVASQDTVAADGSAKRAAALLKSVSAGTPAATAGLKVG
                     DAITAVDGEQIDGSLSLVAQIREREVGDSVTLTVLRQGQRSQIKVTLTARPSTTG"
BASE COUNT          207 a          524 c          582 g          214 t
ORIGIN      
        1 acctggcccg gcggatcaac gcgagtggcc ttcggctgcg taagcacaag ggagaacagg
       61 ggatcggccg tttcgtgggc gtccccatcg acggcggcgc cacggccgac atcgacctcg
      121 tcgcctcgac gtcgcccagc ccggaggcgg tgaccggcgc cgcagcggtc ctcgcctcgg
      181 atctcgaggg gtcagtcctg ctcgtgcaca gcgtgcgccg gggcacctgg gactgccccg
      241 gcggacgccg cgaggagggc gaacgcgtcg tcgactgcgc gcgccgcgag gtgctcgagg
      301 agaccggact cgggctcgag gagagcgagc tcgggtatgc cggatacgag cgcctcacgg
      361 tcaccgaccc cgggcactgg gcctacccga gtcccttcgt ccaggtcttc caggcgcggc
      421 tggggttgct gcgtccgcag ctggcggtcg gtgccgacga cgtcgatggt gctcggtggg
      481 tcagcgagcg cgagctcgac gacctgtgca gccggctctt ctggtggccg ctggcacggc
      541 acctgctcag ccgcgaaccc cgacgatctc gctgagcacg gccaccgacc gggcgaggtt
      601 gacgcgggcc tgcgggtccc aggcggcctg ggcatgcggc gtgcgataga tcgggtcgcg
      661 ggccaggaag gtcgccagga ccgcccggcg ccccctcgcg aactccaggg cgctcacctg
      721 ggcgtactcg gcgcggatct gcagggtgta ctcgtcatac cgggccggtg gcgaggccag
      781 gatccacagg tcggcgtcgt ggaacgcatc ggtcaccggg tcgatgccct ggggatcgtg
      841 atccacggtc atctcgacca ggtcgacgac gcgctcgacg tgatgagggg cggcgttgag
      901 ccggcccagg gtgtggcggg ccagcgcggc gctcgccgcc tcgttggcgc cggccccggc
      961 tcgcgggtcg tagaccgcgt cgtggaacca ggcggccatg cgcgccagcg agacgtcgag
     1021 gccccggagc aggccggcgc tcgtcagctc ggtgagcgcg gcaaggacct cggtgaggtg
     1081 agtggtgcag tgatacgccc gggctggctc ggaccatcgg tcgagcagct catcaccgag
     1141 gtcgcgggcc acgtcggccg agccctgcgg acagagcacg gcacagtcac ccagccaggc
     1201 cagctgcagc ggatgcatgc cggcaggcta cccggtcgtc gagggccgcg cggtgagggt
     1261 gaccttgatt tgtgaccgct ggccctggcg caggaccgtc agggtgacgc tgtcgcccac
     1321 ctcgcgttcg cggatctggg cgaccagtga cagcgagccg tcgatctgct cgccgtcgac
     1381 cgcggtgatc gcgtcgccga ccttgagccc ggccgtcgcc gcgggagttc ccgcggacac
     1441 ggacttgagc agcgcggcgg cccgcttggc gctcccgtca gcggccacgg tgtcctgcga
     1501 cgccacgccg aggtaggcgt gctgagc
//
LOCUS       DATAIX010000018         3000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_208863, whole genome shotgun sequence.
ACCESSION   DATAIX010000018 DATAIX010000000
VERSION     DATAIX010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208863"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1451)
                     /locus_tag="VHM65_00305"
     CDS             complement(<1..1451)
                     /locus_tag="VHM65_00305"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEX2704175.1"
                     /translation="MQTTPLLISQMLRFGTTLHTDQEVVTWTADGPRRTSYREVGVQA
                     AQLAHALRGLGVSGDDRVATFMWNNTEHLTAYLAVPSMGAVLHALNIRLFPDQIVYIA
                     NHGGAQTVIVDNTLAAPLARLLAHLPRIRHVIVNGTVDDETRDALAEPEHVEGVHDYA
                     TLLAGQPESYDWPTDIDENTASSMCYTSGTTGNPKGVSYSHRANYLHALTCAMTLGVR
                     EGDRLLVVVPLFHANAWGFPYLAMAAGASLMMPDRFLQAAPLAEFINTEKISFGAGVP
                     TIWSDLLRYLDAEAVDVSSCRGLMVGGAAAPPALMKAFKQRHGITITHGWGMTEMSPV
                     GSLSLPPSRLEEDSDDYWRYATAQGRLLVGVEARLVALDGEEAEWDGQSIGELEVRGP
                     WITASYNTNGTESEDELREMRDKFHDGWLRTGDIGTLTADGFLRLTDRAKDVIKSGGE
                     WISSVDLENAIMAHPDVVEASVVGVPDERWGERP"
     gene            2078..2905
                     /locus_tag="VHM65_00310"
     CDS             2078..2905
                     /locus_tag="VHM65_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEX2704176.1"
                     /translation="MTSAAFPVTVTAANGQVTITAAPKKIVSLSPTATEMLFALGAGA
                     QVIAVDDQSTYPATAPKTKLSGFQPNAEAVAAYAPDLVVFSNDANGLKAALTKLTIPV
                     LELPAAATLEESYVQEATLGTATGHDKEATALVATTKARLAAAVASVPRTTRAPKVYH
                     ELDPTYFSVTSKTFIGNLYTTLGLVSIADGAGDTSGGYPKLSAEFVITSAPDLVVLAD
                     SKCCGQNEASFAKRPGFASLPAVLNGDVVAADDDIASRWGPRVADLAEAVAKEMATP"
BASE COUNT          433 a         1059 c         1045 g          463 t
ORIGIN      
        1 ggccgttcgc cccagcgctc gtccgggacc ccgacgaccg aggcctcgac gacatcggga
       61 tgggccatga tcgcgttctc caggtcgacc gagctgatcc actcgccgcc ggacttgatg
      121 acatccttgg cccggtcggt cagccgcagg aacccgtcgg cggtgagagt gccgatgtcg
      181 ccggtgcgca gccagccgtc atggaacttg tcgcgcatct cccgcagctc gtcctcggac
      241 tcggtgccgt tcgtgttgta cgacgcggtg atccaggggc cgcggacctc gagctcgccg
      301 atggactgcc cgtcccattc ggcctcttcg ccgtccaggg cgacgagccg ggcctcgacg
      361 ccgaccagga gtcgcccctg cgcggtggcg taccgccagt agtcgtcgct gtcttcctcg
      421 agccgggacg gggggaggga gagcgagccg accggggaca tctccgtcat accccagccg
      481 tgggtgatcg tgatcccatg gcgctgcttg aaggccttca tcagtgcggg cggcgcggcg
      541 gcgcccccga ccatgaggcc ccggcagctc gacacatcga cggcctcggc gtccaggtag
      601 cgcagcaggt cgctccagat ggtgggcacc cccgcaccga aggagatctt ctccgtgttg
      661 atgaactcgg cgagcggcgc tgcctgcagg aaccggtcgg gcatcatcag gctcgcgccg
      721 gcggccatcg cgaggtaggg gaagccccag gcgttggcgt ggaagagcgg gacgacgacg
      781 agaaggcgat ctccctcgcg cacgcccagg gtcatcgcgc aggtgagtgc atgcaggtag
      841 ttggcgcggt gcgagtagga cacgcccttg gggttgccgg tcgtgccgga ggtgtagcac
      901 atcgacgagg cggtgttctc gtcgatgtcc gtcggccagt cgtacgactc cggctggccg
      961 gcgagcagcg tcgcgtagtc gtggacgccc tcgacgtgct ccggctcagc gagcgcgtct
     1021 cgcgtctcgt cgtcgaccgt cccgttgacg atgacgtgcc tgatgcgcgg caggtgggcc
     1081 aggagcctgg ccaacggcgc ggcgagggtg ttgtcgacga tgacggtctg cgcgccgcca
     1141 tggttggcga tgtagacgat ctggtccggg aagagccgga tgttgagcgc gtgcaggacg
     1201 gcccccatcg acggcaccgc cagataggcg gtgagatgct cggtgttgtt ccacatgaac
     1261 gtcgcgaccc ggtcgtcgcc gctcacgccg agcccgcgca gcgcgtgcgc cagctgcgct
     1321 gcctggacac cgacctcacg gtatgacgtg cgccgcggcc cgtccgcggt ccaggtcacc
     1381 acctcctggt cggtgtgcaa cgtcgtgccg aaacgcagca tctggctgat gagcaggggc
     1441 gtggtctgca tcgtgctgtc catgcccaca gcctgccact gcacgccgat ctcggcatgc
     1501 gcatcgctgg catcggcgcg ctgagaggcg cttggcgtag gcgtgtggct cgctatgccg
     1561 tgcccgccag tggcgggcac ggcatagcgg cggaattggg tgatcgctcc aagcgacaga
     1621 aggtggtggc gggactgacg gtggcggcgg ggcgcagcgc ggtgggtgtg gcgcgcgcgg
     1681 ggcgacggtg gaggcccacg cgacagggac ggcgcgcccc cggcggtggt agcctcagcg
     1741 gatcggtccg ggcacgcccg cgccgatgac gcaggcccag cgaagtccgg tcaaaccggc
     1801 gctgtcccgc aactgtgagc cgcctccgcg aagggggttg gtgagccagg ccgcctgtct
     1861 tgcgtctgac aacctcgacc ctcgaggcaa gggtcgttcg ttgggcgtgg aaacccacgg
     1921 gcaccctgcc gctcatagac ggagcactgc attgtcccgg ttccactgtc atcgccccct
     1981 ggccctgctc gctgccgcca gtgtcgccct gctcgccgcc tgcgggtcct ctggctcgac
     2041 cgcccccgcc gcgtccagct cctcaacctc ctccggtatg acgtccgcgg ccttcccggt
     2101 caccgtcacc gccgcgaacg gccaggtgac catcaccgcg gcccccaaga agatcgtgtc
     2161 gctgtccccg acggcgaccg agatgctctt cgctctcggc gcgggcgccc aggtcatcgc
     2221 cgtcgacgac cagtcgacct acccggccac cgcgccgaag accaagctgt ccgggttcca
     2281 gcccaacgcc gaggcggtcg cggcatacgc gcctgatctc gtcgtcttct ccaacgacgc
     2341 caacgggctc aaggcggcgc tgaccaagct gacgatcccg gtgctcgagc tgccggcggc
     2401 ggcgacgctc gaggagagct acgtccagga ggcgacgctc ggcacggcca ccggccacga
     2461 caaggaggcc acggccctgg ttgcgacgac caaggcgcgc ctcgcggccg cggtcgcgtc
     2521 ggtgcccagg acgacgaggg cgcccaaggt ctaccacgag ctcgacccga cgtacttcag
     2581 cgtcacgagc aagaccttca tcggcaacct ctacaccacg ctcggcctcg tctcgatcgc
     2641 ggacggcgcc ggcgacacgt cgggcggcta ccccaagctc tcggccgagt tcgtcatcac
     2701 gtcggcgccc gacctcgtcg tcctggccga ctccaagtgc tgcggccaga acgaggcgtc
     2761 cttcgccaag cgccccgggt ttgcctcgct gccagcggtc ctcaacggtg acgtggtcgc
     2821 tgccgacgac gacatcgcct cgcgctgggg cccccgcgtc gccgacctcg ccgaggccgt
     2881 cgccaaggag atggccaccc cgtgacggtg acccgatgct gacccgccgg ccctgggcgc
     2941 tggctaccac gacagtggcg ctcgtcgtgg tagccgtcct ggccgggatc agtgtcgggg
//
LOCUS       DATAIX010000019         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_30783, whole genome shotgun sequence.
ACCESSION   DATAIX010000019 DATAIX010000000
VERSION     DATAIX010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30783"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VHM65_00315"
     CDS             complement(<1..308)
                     /locus_tag="VHM65_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2704177.1"
                     /translation="MNRLDGRVALVTGGASGIGKATALRLASEGASLVVTDITDEAGA
                     GVAAQIGDAGGRAVFVHHDVTSEIDWLTAVSLAAEEFGGLDILVNNAGTGDLATIEET
                     "
     gene            456..1472
                     /locus_tag="VHM65_00320"
     CDS             456..1472
                     /locus_tag="VHM65_00320"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2704178.1"
                     /translation="MTSEEGPSVRWGILATGSIAATVASDLALVAGAERYAVASRDGA
                     RARDFALQHGFARSYGSHAALLADPDVDAVYIATPHGQHHAVASAVIEAGKPALVEKA
                     FTCSAAAAADLVEKARAADVFLMEAMWLRFQPGHARVRALVAEGAIGEVRAIHADLGF
                     NATAAGRAEGRLTDPAAGGGALLDCGVYVVSLAQWYLGHPSTVHAVGRIGPTGVDVEA
                     GLLLGYPNDAHAILSCSFTTDTPGGATIVGTDGRIIIEPRLHNPRRIRLEREGLDTET
                     IPDPRIGRGYAHEFTHVGECLEAGLTESPVMPLADTLSVMATLDAALDQIGAPHLDEG
                     FTRP"
     gene            1586..>2211
                     /locus_tag="VHM65_00325"
     CDS             1586..>2211
                     /locus_tag="VHM65_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX2704179.1"
                     /translation="MTSIPTRPVRIGVQIQPQHAEYAQIRDAVRDAEDLGVDIVFNWD
                     HFYPLYGEPEGKHLECWTMLGAWAEQTSRVEIGALVTCNSYRNPDLLADMARTVDHIS
                     GGRLVLGIGSGWFEKDYDEYGFEFGTAGGRLDTLAAALPRIESRLARLNPAPTRDIPV
                     LIGGGGERKTLRLVAKHADIWHGFGDPAVVERKVRILDEHCAAVGRDPA"
BASE COUNT          313 a          817 c          764 g          317 t
ORIGIN      
        1 cttgtctcct cgatcgtcgc gaggtcaccc gtaccggcat tgttgacgag gatgtcgagg
       61 cccccgaact cctcggccgc cagcgagacg gccgtgagcc agtcgatctc cgacgtcacg
      121 tcgtggtgga cgaagaccgc cctcccgccg gcatcgccga tctgggccgc cacgccggcc
      181 ccggcctcgt cggtgatgtc ggtgacgacg agcgatgccc cttcactcgc gaggcgcagg
      241 gccgtggcct tcccgatccc gctggctccc ccggttacga gcgcaacgcg cccatcgagc
      301 ctgttcatgt caggactccg tccttgtcgg ccgatggccg gatcgtcctg ccggggcacg
      361 gcgctcgcca gggccattgg tccccagcgc cggttcgcgg cccggctcgc tgcgtagact
      421 cggtcctcga cctgacgtcc gccgcgggag gcagcatgac cagcgaggag ggaccgtcgg
      481 tgcgctgggg gatcctggcc acgggttcca tcgccgcgac cgtcgcgagc gaccttgcgc
      541 tcgtggcggg ggcggagcgg tatgccgtcg catcgcgcga cggcgcgcgc gcgagggact
      601 tcgctctcca gcacggcttc gcgcgctcct acggctccca cgccgcgctg ctcgccgacc
      661 ctgacgtcga cgccgtctac atcgcgacac cccatggcca gcaccacgcg gtcgcgagcg
      721 cggtcatcga ggcgggcaag cccgctctcg tcgagaaggc gttcacctgc tcggccgccg
      781 ccgctgcgga cctcgtcgag aaggcccggg ccgctgacgt gttcctcatg gaggccatgt
      841 ggctgcgctt ccagccgggg catgctcgag tgcgcgcgct ggtcgccgag ggggcgatcg
      901 gtgaggtccg cgccatccat gccgatctcg gcttcaacgc cacggcggca gggcgggccg
      961 aggggcggct caccgacccg gccgctggtg gtggcgccct cctcgactgc ggggtgtatg
     1021 tcgtatccct ggcacagtgg tatctcggcc acccgtcgac cgtccacgcg gtcggccgga
     1081 tcggcccgac cggggtcgac gtcgaggcgg ggctgttgct cggctacccc aacgacgcgc
     1141 acgcgatcct gtcctgcagc ttcacgacgg acacgcccgg cggagcgacg atcgtcggca
     1201 ccgacggacg catcatcatc gaaccccggc tacacaaccc ccggcgaatc cggttggagc
     1261 gagaaggact cgacaccgag accatccccg acccacgcat cggcaggggc tatgcacacg
     1321 agttcacgca cgtgggggag tgcctcgagg ccgggctgac cgagtcaccg gtgatgccac
     1381 tggctgacac gctctcggtc atggcgaccc tggacgcggc cctcgaccag atcggcgccc
     1441 cccacctcga cgagggcttc acccggccgt aggacggtcg accgggacgg cgtgctgccg
     1501 tgggcgtgag ccgccggctc ggggatctgg gcaggcgacg cgcgacgggg cgccgcggcg
     1561 cgcgcgtccg gcataccgtg gtcgcatgac gagcatcccc acccgcccgg tccgcatcgg
     1621 cgtgcagatc cagccccagc acgccgagta cgcacagatc cgcgacgcgg tccgcgacgc
     1681 cgaggacctc ggcgtcgaca tcgtcttcaa ctgggaccac ttctacccgc tctacggcga
     1741 gcccgagggc aagcacctcg agtgctggac gatgctcggg gcctgggccg agcagacgag
     1801 ccgcgtcgag atcggcgcgc tcgtgacgtg caacagctat cgcaacccgg acctgctggc
     1861 cgacatggcc cggaccgtcg accacatcag tggcgggcgg ctcgtgctgg ggatcggctc
     1921 gggctggttc gagaaggact acgacgagta cgggttcgag ttcggcacgg ccgggggccg
     1981 gctcgacacc ctggccgcgg cccttccccg catcgagtca cggctcgcca ggctcaaccc
     2041 ggccccgacc cgcgacatcc ccgttctcat cggtgggggc ggcgagcgca agaccctgcg
     2101 tctcgtcgcg aagcatgccg atatctggca cggcttcggc gacccggccg tggtcgagcg
     2161 caaggtgcga atcctcgacg agcactgcgc cgccgtcggc cgcgaccccg c
//
LOCUS       DATAIX010000020         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_60629, whole genome shotgun sequence.
ACCESSION   DATAIX010000020 DATAIX010000000
VERSION     DATAIX010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60629"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VHM65_00330"
     CDS             complement(<1..451)
                     /locus_tag="VHM65_00330"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEX2704180.1"
                     /translation="MSNASAPKARRAILPGIFQGLRTTARTLVKPTHTQEYPDVQPSL
                     PPRTRGVIALTQENCTSCMLCARECPDWCIYIDSHKETIPATTPGGRERQRNVLDRFA
                     IDFSLCMYCGICIEVCPFDALHWSPEFEYAEVDVRDLLHEKERLHEWM"
     gene            complement(448..1410)
                     /locus_tag="VHM65_00335"
     CDS             complement(448..1410)
                     /locus_tag="VHM65_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEX2704181.1"
                     /translation="MSILEVVIRAVAVLLVFLVLPLLVGQTEHKAMAHMQGRLGPMYA
                     GGFHGWAQLIADAVKFVQKEDVLPARADKWIYRLAPAVALIPYLVVMAGIPLGPGPNF
                     VGANVDAGALYVLAVAGVGVIGSLMGGWASGSKYSLLGAMRSAAQMMSYELPMVLSVA
                     SVALAAGTLSLSGIVSAWSPWWLLWQLPGAVVFFIGSLAEILRNPFDMPIAESEIVMG
                     HMTEYSGLRFAFFLLTEYAGVVVMSLLFAVFFLGGWQGPAPDALGWLWTLLKGYVIAF
                     IIIWMRVSWPRMREDQLQRLAWLWLVPISLVQLAMTAVGVVMMQ"
     gene            complement(1407..>1609)
                     /locus_tag="VHM65_00340"
     CDS             complement(1407..>1609)
                     /locus_tag="VHM65_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704182.1"
                     /translation="FVLTARVSKPWPGAKEPAEGQGHDSGAPAPAGGRRKAQAMGVPD
                     PSWGPRVEAPEAPAAPAEDEAP"
BASE COUNT          278 a          537 c          577 g          217 t
ORIGIN      
        1 ccatccactc gtgcagccgc tccttctcgt gcagcagatc gcgcacgtcg acctcggcat
       61 actcgaactc ggggctccag tgcagcgcgt cgaaggggca cacctcgatg cagatcccgc
      121 agtacataca cagcgagaag tcgatggcga agcggtcgag gacgttgcgc tggcgttccc
      181 gcccaccggg cgtcgtcgcc gggatcgtct ccttgtgcga gtcgatgtag atgcaccagt
      241 cggggcactc gcgggcgcag agcatgcacg aggtgcagtt ctcctgggtc agcgcgatga
      301 ccccgcgggt gcgtggtggc agcgacggct ggacgtcggg gtactcctgg gtgtgggtcg
      361 gtttgaccag tgtcctggcg gtcgtcctca acccctggaa gatgccgggg agtatggcgc
      421 gccgcgcctt gggtgcagaa gcgttgctca ctgcatcatc accaccccga ctgccgtcat
      481 agcgagctgg accaaagaga tgggcacgag ccagagccag gcgagtcgct gcagctggtc
      541 ctcgcgcatc cgcggccagg agacgcgcat ccagatgatg atgaacgcga tgacatagcc
      601 cttgagcagc gtccacagcc agccgagggc gtcgggtgcg ggtccctgcc atccgccgag
      661 gaagaacacc gcgaagagca ggctcatgac gacgacgccg gcatactcgg tcagcaggaa
      721 gaacgcgaag cgcagcccgg agtactcggt catgtgcccc atgacgatct cggactcggc
      781 gatcggcatg tcgaaggggt tgcgcaggat ctcggcgagc gagccgatga agaagacgac
      841 cgcgccgggc agctgccaga gcagccacca cggcgaccat gccgagacga tgccgctgag
      901 tgagagggtg cccgcggcga gcgccaccga ggcgaccgac aggaccatcg gcagctcgta
      961 ggacatcatc tgcgcggccg atcgcatcgc cccgagcagg gagtacttgc tgccgctggc
     1021 ccagccgccc atgagcgagc cgatgacacc gaccccggcc acggccagga cgtagagggc
     1081 cccggcgtcg acgttggcgc cgacgaagtt cggccccggg cccagcggga tccccgccat
     1141 gaccacgagg taggggatga gcgcgacggc gggggccagc cggtagatcc acttgtccgc
     1201 ccgggcgggg agcacgtcct ccttctggac gaacttcacc gcgtcggcga tgagctgggc
     1261 ccagccgtgg aagccaccgg catacatggg cccgagccgg ccctgcatgt gcgccatcgc
     1321 cttgtgctcg gtctggccga ccagcagcgg gaggacgagg aagacgagga ggacggcgac
     1381 ggcgcggatg acgacttcga ggatgctcac ggggcctcgt cctcggccgg cgcggcaggt
     1441 gcctccggcg cctcgacccg cgggccccac gacgggtcag gcacgcccat ggcctgggcc
     1501 ttgcgacggc cgccggcggg ggccggggcg cccgagtcgt gaccctggcc ctccgccggc
     1561 tccttggccc ccggccacgg cttggagacg cgcgcggtga ggacgaagg
//
LOCUS       DATAIX010000021         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_90459, whole genome shotgun sequence.
ACCESSION   DATAIX010000021 DATAIX010000000
VERSION     DATAIX010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90459"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1463
                     /gene="nuoH"
                     /locus_tag="VHM65_00345"
     CDS             <1..1463
                     /gene="nuoH"
                     /locus_tag="VHM65_00345"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX2704183.1"
                     /translation="LALPLLTTPLLTTPLVAPASTDNPAVSFADTPLWLSLVKALLIF
                     VFLLLNTLMVIWFERRVIGRMQQRPGPNRTGPFGLLQTLADGMKSMFKEDVTPANADR
                     VIFTLAPIIAATMAFVSFAIIPMGPVVSMWGHKTPLQLADVPVAVLLVLAVASVGIYG
                     IVLAGWSSGSTYPLLGGLRSTAQMISYEIAMGLSLVAIFLYSGSMSTSQIVAAQSSLW
                     YMVPAFFSFGVYVVTMVGETNRLPFDLAEGEGELTGGYHTEYGSMRFAMFFLGEYVNM
                     FTVSALATTMFLGGWQAPPFIASINDGMLNQGWWGLLWFTIKMWSFMFFFVWLRGSLP
                     RVRYDQFMRFGWRFLIPVTLAWVVAVSFIRAAQLGYFGGGTVTLFNRTYSTVVLVVIG
                     VIALGAIAVSFVVENRAIAREAAKPIPPEEVDPFAGGFPVPPLPGQRLTEPVYAGAAT
                     ASITAAAPPEGAALGGSDPGGAGTTWYIEPEEDARG"
     gene            1456..2178
                     /gene="nuoI"
                     /locus_tag="VHM65_00350"
     CDS             1456..2178
                     /gene="nuoI"
                     /locus_tag="VHM65_00350"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX2704184.1"
                     /translation="MAEDKVVAPATAGGGGVDIPAAGRSTGGTRKQPSFLASMFAPVA
                     GFGVTFATMFRTLETEEYPEVKRPTAPRFHGRHQLNRHPDGLEKCIGCELCAWACPAD
                     AILVEGADNDDSPAEAGGTGRFSPGERYGRVYQINYLRCIFCGLCIEACPTRALTMTN
                     VYELADDDRAKLIFTKDQLLAAPAEGMVAPPHPMAPGMEERDYYLGRVAKATPEQEAY
                     AAERASRDRAASVDMVSEGGAL"
     gene            2178..>2432
                     /locus_tag="VHM65_00355"
     CDS             2178..>2432
                     /locus_tag="VHM65_00355"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX2704185.1"
                     /translation="MPGTGEAVMFWILAPISVLAALGLLFLRKAVHAALGVALTMVIL
                     GIFYLAQQADFLGIVQVFVYTGAVMMLFLFVLMLIGVDSSD"
BASE COUNT          314 a          888 c          788 g          442 t
ORIGIN      
        1 cgctcgcact tcccctgctg actacccctc tgctgactac ccccctggtg gctcccgcct
       61 cgaccgacaa ccccgcggtg agctttgccg acacccccct gtggctctcg ctcgtcaagg
      121 cgctgctcat cttcgtcttc ctgctgctca acaccctcat ggtgatctgg ttcgagcgcc
      181 gggtcatcgg ccgtatgcag cagcgtccgg gccccaaccg caccggcccg ttcggcctgc
      241 tgcagaccct tgccgacggc atgaagtcga tgttcaagga ggacgtcacc cccgccaacg
      301 ccgaccgggt catcttcacc cttgccccga tcatcgccgc gacgatggcc ttcgtgtcct
      361 tcgcgatcat cccgatgggg ccggtcgtgt cgatgtgggg tcacaagacg cccttgcagc
      421 tcgccgacgt gcccgtcgcc gtcctgctcg tgctggccgt cgcgagcgtc ggtatctacg
      481 gcatcgtgct cgcgggctgg tcctcgggct cgacctaccc gctgctcggt gggctgcgct
      541 cgaccgccca gatgatctcc tacgagatcg ccatgggcct ctcgctcgtc gcgatcttcc
      601 tctacagcgg ctcgatgtcg acctcgcaga tcgtcgcggc acagtcctcg ctctggtaca
      661 tggtgccggc cttcttctcc ttcggcgtct acgtcgtcac gatggtcggc gagaccaacc
      721 ggctgccgtt cgacctcgcc gagggcgagg gtgagctgac cggtggctac cacaccgagt
      781 acggctcgat gcggttcgcg atgttcttcc tcggtgagta cgtcaacatg ttcaccgtct
      841 cggccctggc cacgacgatg ttcctcggcg gctggcaggc gccgccgttc atcgcctcga
      901 tcaacgacgg catgctcaac cagggctggt ggggcctgct ctggttcacc atcaagatgt
      961 ggtcgttcat gttcttcttc gtgtggctgc gcgggtcgct gccccgggtg cgctacgacc
     1021 agttcatgcg cttcggctgg cgcttcctca tcccggtgac cctcgcctgg gtcgtcgcgg
     1081 tgtcgttcat ccgtgccgcc cagctcggct acttcggcgg cggcacggtg accctgttca
     1141 accgcaccta ctccacggtc gtcctcgtcg tcatcggggt catcgcgctc ggcgccatcg
     1201 ccgtctcgtt cgtcgtggag aaccgcgcga tcgcccggga ggccgccaag cccatcccgc
     1261 cggaggaggt cgacccgttc gccggcggct tccccgtccc gccgctgccc gggcaacggc
     1321 tcaccgagcc ggtgtatgcc ggtgccgcca ccgcgagcat cacggcggcc gcgccgcccg
     1381 agggcgctgc cctcggtggg tccgacccgg gcggggccgg cacgacgtgg tacatcgagc
     1441 ccgaggagga cgcccgtggc tgaggacaag gtcgtggccc cggccaccgc gggaggtggc
     1501 ggcgtcgaca taccggctgc cggccggtcg acgggtggca cgcgcaagca gcccagcttc
     1561 ctcgcctcga tgttcgcgcc cgtggcgggc ttcggggtca ccttcgcgac gatgttccgc
     1621 acgctggaga ccgaggagta ccccgaggtc aagcggccca ccgcgccgcg cttccacggc
     1681 cgccaccagc tcaaccggca cccggacggg ctggagaagt gcatcggctg cgagctgtgc
     1741 gcctgggcct gcccggccga cgccatcctc gtcgagggcg ccgacaacga cgactcgccc
     1801 gccgaggcgg gaggcaccgg acggttctcg cccggcgagc gctacggccg ggtctaccag
     1861 atcaactatc tgcgctgtat cttctgcggg ctctgcatcg aggcgtgccc gacccgggcc
     1921 ctgacgatga cgaacgtcta cgagctggcc gacgacgacc gggccaagct gatcttcacc
     1981 aaggaccagc tgctcgcggc gccggccgag gggatggtcg cgccaccgca cccgatggcc
     2041 ccgggcatgg aggagcgcga ctactacctc gggcgggtcg ccaaggccac gccggagcag
     2101 gaggcgtatg ccgccgagcg ggcctcccgg gaccgggcgg catcggtcga catggtctct
     2161 gaggggggtg cgctctgatg cccggaaccg gtgaggcggt gatgttctgg atcctcgccc
     2221 ccatcagcgt gctggcggca ctcgggctgc tcttcctgcg caaggcggtg cacgcggctc
     2281 tcggggtggc gctgacgatg gtcatcctcg ggatcttcta cctcgcccag caggccgact
     2341 tcctcggcat cgtgcaggtc ttcgtctaca ccggcgcggt catgatgctg ttcctcttcg
     2401 tgctcatgct catcggcgtc gactcctcgg ac
//
LOCUS       DATAIX010000022         4057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_268640, whole genome shotgun sequence.
ACCESSION   DATAIX010000022 DATAIX010000000
VERSION     DATAIX010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268640"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VHM65_00360"
     CDS             complement(<1..599)
                     /locus_tag="VHM65_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704186.1"
                     /translation="MSEPRPAAAGPAPELAITGGPGSFAADLQDVTTAAHVLDRAGDG
                     LREAAGRVTRLMATGDLLAAAPICPISTAVAEARIAATVVGPFGLLPLSALCEGMARG
                     IAATVATYQAGDLAAAAGFEAARHQLGALLGSVTVVSAVAVAADPGLALALLFGGAAW
                     AGADGPAAAAEALAGHPEVLDTVIGLLPGYVEGAVMTAPS"
     gene            complement(596..1315)
                     /locus_tag="VHM65_00365"
     CDS             complement(596..1315)
                     /locus_tag="VHM65_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704187.1"
                     /translation="MSGLQWVIDDEVIRARLPEGVTATDVTGPELARARTTLTNPLVS
                     LVLQSRSPVGHSVTNVLIGAVVVSLSTQVPSPDGPGHAFAAARFVEVSIAPLTRCLDE
                     IMRVIPGPGRCGAGEAGTVALGLRSAARQDRLAALWLVRDRHYLFLRPGPGATPQVMS
                     RAQVSQVILSELAEALVLGLVDPDFDLDGPDADPDGPDAHPDGHGWRDDPDADHSDAD
                     VDHSDADHSDERDRRPGTARA"
     gene            complement(1312..1779)
                     /locus_tag="VHM65_00370"
     CDS             complement(1312..1779)
                     /locus_tag="VHM65_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704188.1"
                     /translation="MLGGDPDRLRDLARALRQESGEVSAAADDVRHARGVTWESTAAD
                     LCRDALGRQAGLIDMCAEVVHDAAGSVDALADELAERQRAIRAAQRLVLGLLTQAQET
                     VWRFAGVPAHELGEAGAAARSAAEALLDTARDLPGPGSPDWLGLARTLRRGRS"
     gene            complement(1949..>4057)
                     /locus_tag="VHM65_00375"
     CDS             complement(1949..>4057)
                     /locus_tag="VHM65_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX2704189.1"
                     /translation="EFLGHTRMSRLPVVAAAHWVTMIGFMVLFLTLLNAFGQLIEPEF
                     ALPLIGHFPPYEWVTDLFAFIGFWAIIVLIVIRQRNHPRSAPGAGGRRSRFFGSTFWQ
                     AYYVELTILAVMSCIGLLRAFEWTLARQAGSDLASALHFPVTGWVGSWFTGVPTETIT
                     TFIVALAAIKICISFAWMIIIALTPTMGVAWHRFLAFFNIFFKRHADGRTALAALQPI
                     LVGGQALDFEKVDELGEDDALGVGKIEDFTWKGLLDFTTCTECGRCQSQCPAWNTDKP
                     LSPKLLVMTLRNHAHAKAPWLLAAESARDALPADVKAAAAVPALVGSTGYDVNSPLSA
                     YTMLGPDAVIDEDVLWSCTTCGACVEQCPVDIEHVDAIVDMRRYKVLIESAFPSELNS
                     LFRNLEKNQNPWGMSSRLRLDWAKDLPFDVPVVGADVEDLTEVDYLFWVGCAGAYEDR
                     AKKTTRAVAELLHTAGVTFAVLGDGEACTGDSARRSGNEFLFQMLAQGNVEMLGEVKA
                     TKIVVTCAHCFNTIKNEYPQLGGHYEVVHHTQLLNQLVRDKLLVPVSRPDGGPSMSSA
                     RNAASTAASVTYHDPCYLGRHNHVYAPPRELLGALPGVELTEMPRHGEKSFCCGAGGA
                     RMWIEEKLGTRINTNRTDEAIATGARRIAVGCPFCKVMLSDGLTAKATQDTSLADVEV
                     VDVAQMLLAAVRRSETVTTG"
BASE COUNT          582 a         1418 c         1495 g          562 t
ORIGIN      
        1 gagggcgccg tcatgacggc gccctccaca tagccgggaa gcaggccgat cacggtgtcg
       61 agaacctcgg gatggccggc gagcgcctcg gccgcggcgg ccggcccgtc ggcaccggcc
      121 cacgcggccc cgccgaacag cagcgcaagg gccagcccgg ggtcggccgc cacggccacc
      181 gccgagacca ccgtgaccga tcccagcagg gcgcccagct ggtggcgcgc ggcctcgaag
      241 cccgccgcag cggccagatc gccggcctgg tatgtcgcga ccgtggccgc gatcccgcgc
      301 gccatcccct cgcacagtgc cgacagtggg agcagcccga acgggccgac gaccgtggcc
      361 gcgatccggg cctcggcgac cgccgtcgag atggggcaga tcggcgcagc ggcgagcagg
      421 tctcccgtcg ccatgagccg ggtgacccgg ccggccgcct cacgcagacc gtccccagcc
      481 cggtccagca cgtgggcggc ggtcgtgacg tcctgcaggt cggcggcgaa gctgcccgga
      541 ccgcccgtga tggcaagctc gggcgccgga cctgcggctg caggcctggg ctcgctcatg
      601 ctctcgccgt gccaggtcgc cggtcccgct cgtcgctgtg gtcggcgtcg ctgtggtcga
      661 cgtcggcgtc gctgtggtcg gcgtccgggt cgtcgcgcca gccgtggccg tcggggtggg
      721 cgtccgggcc gtcggggtcg gcgtccgggc cgtcgaggtc gaagtcgggg tcgacgaggc
      781 cgagcacgag ggcttcggcc agctcgctga ggatgacctg gctcacctgc gctcgactca
      841 tcacctgcgg cgtcgcacct ggcccggggc gcaggaacag gtagtggcgg tccctgacga
      901 gccagagagc agccagccgg tcctgccgtg ccgcagatcg caggcccagg gccacggtcc
      961 cagcctcgcc ggcaccgcag cggcccggcc ccgggatgac ccgcatgatc tcgtcgaggc
     1021 aacgggtcag gggtgcgatc gacacctcga cgaagcgggc cgccgcgaag gcgtgtccag
     1081 gtccgtccgg gctcgggacc tgcgtgctca ggctcacgac gacggccccg atcaggacgt
     1141 tggtgacgct gtgcccgacc gggctgcgtg actgcaggac gagtgacacg agcgggttcg
     1201 tcagagtcgt ccgagcccgg gccagctctg gtccggtgac atcggtcgcc gtcaccccct
     1261 cgggcagacg cgcccggatc acctcgtcgt cgatgaccca ctgcagcccg ctcatgaccg
     1321 gcctctccgc agggtgcgcg cgagcccgag ccagtccggc gaccccgggc cggggaggtc
     1381 ccgggccgtg tcgagcagcg cctcggccgc ggaccgggcc gcagcgccgg cctcgccgag
     1441 ctcgtgcgct gggacgccgg cgaaccgcca taccgtctcc tgtgcctgcg tgagcagccc
     1501 gagcacgagc cgctgggcgg cccggatcgc gcgctgacgc tcggccagct cgtcggcgag
     1561 cgcgtcgacg ctgccggcgg cgtcgtggac cacctcggcg cacatgtcga tgaggccggc
     1621 ctgccggccg agcgcgtcgc ggcacaggtc cgcggcggtg ctctcccagg tcacgccgcg
     1681 ggcgtgccgc acgtcgtcgg ccgccgccga cacctcgccg ctctcctggc gcagcgcgcg
     1741 ggcgaggtcg cgcagccggt ccgggtctcc tccaagcatg ccgctgacgt taggtcgcgg
     1801 acaccgccat ccggtatgcc ggtccgctca cctgtggagg gtgggttctg tcagcgaggg
     1861 gctgtggacg agccggacga cctgccaggc tgggcccacg ccccccgccc acgccccggc
     1921 gagcggcgcc cagcggtgcg gatcctcgtc agccggtcgt gacggtctcg ctgcgacgga
     1981 ctgccgcgag gagcatctga gccacgtcga cgacctcgac gtcggccagt gaggtgtcct
     2041 gcgtcgcctt ggccgtcagc ccgtcgctga gcatcacctt gcagaacggg cagccgaccg
     2101 cgatgcgccg ggcgcctgtc gcgatggcct cgtcggtgcg gttcgtgttg atccgggtgc
     2161 cgagcttctc ctcgatccac atccgggcgc caccggcgcc gcagcagaac gacttctcgc
     2221 cgtggcgcgg catctcggtg agctcgaccc cgggcagcgc cccgagcagc tcgcgcggcg
     2281 gtgcgtagac gtggttgtgc cggccgaggt agcacgggtc gtggtaggtc accgacgcgg
     2341 ccgtcgacgc ggcgttcctg gccgacgaca tcgacgggcc gccgtcgggg cgggacaccg
     2401 ggacgagcag cttgtcgcgc acgagctggt tgagcagctg cgtgtggtgg acgacctcgt
     2461 agtgcccgcc gagctggggg tactcgttct tgatcgtgtt gaagcagtgc gcgcaggtga
     2521 cgacgatctt ggtcgccttg acctcgccga gcatctcgac gttgccctgc gcgagcatct
     2581 ggaagaggaa ctcgttgccc gatcgccggg ccgagtcacc cgtgcaggcc tcgccgtcac
     2641 cgagcaccgc gaacgtcacc ccggccgtgt gcagcagctc ggccacggcg cgggtcgtct
     2701 tcttcgcccg gtcctcgtag gccccggcgc agccgaccca gaagaggtag tcgacctcgg
     2761 tgaggtcctc gacgtcggcc ccgacgaccg gcacgtcgaa cgggaggtcc ttggcccagt
     2821 cgaggcgcag ccgcgacgac ataccccagg ggttctggtt cttctccagg ttgcggaaca
     2881 gcgagttgag ctcggagggg aacgccgact cgatgaggac cttgtagcgg cgcatgtcga
     2941 cgatggcgtc gacgtgctcg atgtcgaccg ggcactgctc gacgcacgca ccgcacgtcg
     3001 tgcacgacca caggacgtcc tcgtcgatga ccgcgtccgg accgagcatc gtgtatgccg
     3061 agagcggcga gttcacgtca tacccggtgg agccgacgag ggctgggacc gcggcggcgg
     3121 ccttgacgtc ggcgggcagc gcgtcgcggg cggactcggc ggccagcagc cacggggcct
     3181 tggcgtgcgc gtggttgcgc agtgtcatga cgagcagctt gggggacagc ggcttgtcgg
     3241 tgttccaggc cgggcactgg ctctggcagc ggccgcactc ggtgcacgtc gtgaagtcga
     3301 gcaggccctt ccacgtgaag tcctcgatct tgccgacgcc gagcgcatcg tcctcgccga
     3361 gctcgtcgac cttctcgaag tcgagggcct gcccgcccac caggatcggc tgcagggccg
     3421 cgagcgcggt ccggccgtcg gcgtgccgct tgaagaagat gttgaagaac gcaaggaagc
     3481 ggtgccatgc cacgcccatc gtcggggtga gcgcgatgat gatcatccac gcgaaggaga
     3541 tgcagatctt gatggccgcg agggcgacga tgaacgtcgt gatcgtctcg gtcggcacac
     3601 cggtgaacca ggagccgacc cagccggtga ctgggaagtg cagggcgctg gccaggtccg
     3661 agcccgcctg cctggccagc gtccactcga aggcgcggag caagccgatg caggacatga
     3721 cggcgaggat cgtcagctcg acgtagtagg cctgccagaa cgtcgacccg aagaagcggc
     3781 tgcggcgacc acccgcaccc ggtgccgagc gcgggtggtt gcgctggcgg atgacgatga
     3841 gcacgatgat cgcccagaag ccgatgaagg cgaacaggtc ggtgacccac tcgtatggcg
     3901 ggaagtgccc gatcagcggg agcgcgaact cgggctcgat gagctggccg aaggcgttga
     3961 gcagggtgag gaagagcacc atgaagccga tcatcgtcac ccagtgcgcc gccgccacga
     4021 cgggcaggcg cgacatccgg gtgtggccga ggaactc
//
LOCUS       DATAIX010000023         4585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_1093, whole genome shotgun sequence.
ACCESSION   DATAIX010000023 DATAIX010000000
VERSION     DATAIX010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VHM65_00380"
     CDS             <1..242
                     /locus_tag="VHM65_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX2704190.1"
                     /translation="SLADIPDGTVVDVVDCFVRSDLVGAVVDAAIAQRERLGIGAVWL
                     QLGVVDEDAAARAKAAGLAVVMDTCPLIEWPLVTR"
     gene            complement(358..897)
                     /gene="purE"
                     /locus_tag="VHM65_00385"
     CDS             complement(358..897)
                     /gene="purE"
                     /locus_tag="VHM65_00385"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX2704191.1"
                     /translation="MSAVTQRPAGPVVGLVMGSDSDWPVMGEAARALEEFGISYEADV
                     VSAHRMPEDMIRYGADAESRGLRVIIAGAGGAAHLPGMLASVTTLPVIGVPVPLAHLD
                     GMDSLLSIVQMPAGVPVATVSIGGARNAGLLAARILSAGEGAEAVRLRQDMASFQRRL
                     RDEARRKGESLRRQSPPDG"
     gene            complement(894..2087)
                     /locus_tag="VHM65_00390"
     CDS             complement(894..2087)
                     /locus_tag="VHM65_00390"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004680.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX2704192.1"
                     /translation="MTAPLRAPGGFPVVGVIGGGQLARMLQGPAVELGVQLSVLAEAP
                     DASAALVIPSAPVGDHADVEAVLAFARHCDVVTFDHEQVPAHVLAALESAGVPLHPTP
                     AALVYAQDKLAMRSRLEQLGIRVPAWAQARTMTEIEAFGARAGWPMVAKAPRGGYDGK
                     GVRVVASGSELADWVAAVGVPGPLARGFLLEEHVAFVRELAVLVARSPTGQAAAWPVV
                     ETVQTDGICTQVIAPAGALDPGLAATVTEAGLRIAGSLAVTGVLAVELFEVRDGEGAP
                     SYVVNELAMRPHNSGHWSMDGAVTGQFEQHLRAVLDLPLGDPRPRAPYTVMANVLGGA
                     HEELYSAYRHVMAHDPGVKVHLYGKSVRPGRKIGHVNVSGTHLGPLVERAAHAAGYLA
                     GVVTE"
     gene            2161..2643
                     /locus_tag="VHM65_00395"
     CDS             2161..2643
                     /locus_tag="VHM65_00395"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX2704193.1"
                     /translation="MTQHAGLGGRLRDAFELIRRELVRFGLVGAMAFVVEIALFNLFL
                     HVLIRDKVTTSKIVAASVATLCAWLGNRYWTFRQRSTGSLRRELVLFFAVNGLAIAVG
                     AAWLAFTHYVLGLDSRLQDNISNVFGIGLGTLLRFWAYHELVFAEVSETQPLTGTTRT
                     "
     gene            complement(2603..3634)
                     /locus_tag="VHM65_00400"
     CDS             complement(2603..3634)
                     /locus_tag="VHM65_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2704194.1"
                     /translation="MRRQLARAALAAVAVAFTVVMGPVTAAVLWVAAARPDIVTDWLD
                     QRAAVRLGQLSAEVFASFALTLGLTALLTYRTTRRFSDAMAGLVTSAEQLGEGRSKVE
                     PMRSGIPEVDRVSDVLVRSGQHVRASLAAERDFAADASHQLRTPLTALLMRLEEVAAT
                     DDLDVVHEEAGIAMGQVERLTRVVDDLLMRSRRANGGPLPEVSLDSVIASLQREWQPA
                     FENARRSIRVDGERGLVVRAMPGALSQVLSTLLENSLVHGLGTVDVRARRSGPSVVVE
                     VSDHGDGIAATIAAHIFQRSVSTGQGKSTGLGLALARDLAESNGGRLDLISARPAKFA
                     LFLSEAESR"
     gene            complement(3641..4315)
                     /locus_tag="VHM65_00405"
     CDS             complement(3641..4315)
                     /locus_tag="VHM65_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2704195.1"
                     /translation="MTRVLLAEDDPAISEPLARALRREGYEVDVRADGPSALAGARNN
                     PDLLVLDLGLPHLDGLEVCRRIRAEGITVPVLVLTARADEVDTVVGLDAGADDYVTKP
                     FRLAELLARVRALLRRSPVEDSGAELVRIDQDGRRAWFHDEELVLTAKEFDLLRVLVR
                     EQGKVVSREQLMREIWETAWFGSTKTLDMHISVLRKKLGDDAAQPRYIATVRGVGFRF
                     ERLDRG"
     gene            complement(4337..>4585)
                     /locus_tag="VHM65_00410"
     CDS             complement(4337..>4585)
                     /locus_tag="VHM65_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2704196.1"
                     /translation="VDEVVPAGTARDRALEIAAEIARNSPVGLRQAKRAMRLGFDVDL
                     ATGLEVEDSCWRATAFSGDRAEGVAAFAEKRDPRWPGE"
BASE COUNT          672 a         1670 c         1614 g          629 t
ORIGIN      
        1 ccagccttgc cgacatcccg gacggcaccg tggtcgacgt cgtcgactgc ttcgtgcgct
       61 ccgacctcgt cggagcggtc gttgatgcgg cgatcgcgca gcgggagcgg ctggggatcg
      121 gcgcggtgtg gctccagctc ggtgtcgtcg acgaggacgc cgcggcccgg gccaaggcag
      181 ccggcctcgc ggtcgtcatg gacacctgcc ccctgatcga gtggcccctg gtcaccaggt
      241 agatccaccc tccgccgagg tcgggcaagg tccgcccatc cggcctggat gagcggacct
      301 tgcccgactt cgagcgacgg cccgacgtcg agcgacagcc ccgggtcgcg gcgcggtcta
      361 cccgtctggc ggggactgac gacgcaggct ttcacccttg cgccgggcct catcacgcag
      421 ccgacgctgg aaggacgcca tatcctggcg cagccgcacc gcctcggcac cctccccggc
      481 cgagaggatg cgcgccgcga gcagccccgc gttgcgggcg ccgccgatcg agaccgtcgc
      541 caccggcaca ccggccggca tctggacgat cgagagcagc gagtccatcc cgtcgaggtg
      601 ggccagcggc accggaaccc cgatcaccgg cagcgtcgtc accgaggcga gcatgcccgg
      661 caggtgcgcc gcgccgccgg cccccgcgat gatgacgcgc agcccgcgcg actccgcgtc
      721 cgcgccatac cggatcatgt cctcgggcat ccggtgcgcc gagacgacgt cggcctcgta
      781 ggagatcccg aactcctcga gcgcccgcgc cgcctcgccc atgacgggcc agtcgctgtc
      841 gctgcccatg acgagcccga cgaccgggcc ggccggtcgc tgcgttaccg cgctcactcg
      901 gtcaccactc ctgcaaggta tcccgccgca tgcgcggccc gctcgacgag cggtcccagg
      961 tgggtcccgc tcacgttgac gtgcccgatc ttgcgccccg gccgcaccga cttgccgtag
     1021 aggtgcacct tgacgcccgg gtcatgagcc atgacgtgcc ggtatgccga gtacagctcc
     1081 tcgtgcgcgc cgcccagcac gttggccatg accgtgtatg gcgcgcgcgg ccggggatcg
     1141 ccgagcggca ggtccaggac cgcgcgaagg tgctgctcga actggccggt caccgcgccg
     1201 tccatgctcc agtggccgct gttgtggggc cgcatggcga gctcgttgac gacgtatgac
     1261 ggcgcaccct cgccgtcacg gacctcgaac agctccaccg cgaggacgcc tgtgacggcg
     1321 agggagccgg cgatccggag ccccgcctcg gtgaccgttg cagccaggcc tggatccagt
     1381 gcgccggccg gtgcgatgac ctgcgtgcag atcccgtcgg tctggaccgt ctcgacgacg
     1441 ggccaggccg cagcctggcc cgtcgggctg cgggcaacga ggacggccag ctcgcggacg
     1501 aaggcgacgt gctcctcgag caggaagccc cgggccagtg gacccggcac gccgaccgcc
     1561 gcaacccagt cggccagctc ggaacccgac gcgacgacgc gaacgccctt gccgtcatac
     1621 ccgcctcgcg gtgccttcgc gaccatgggc cacccggcgc gcgcgccgaa ggcctcgatc
     1681 tcggtcatcg tccgcgcctg tgcccacgcg ggcacccgga tcccgagctg ctccaggcgc
     1741 gatcgcatcg cgagcttgtc ctgggcatag accagcgcgg cgggcgtggg atgcagtggc
     1801 accccggccg attcgagcgc cgccagcacg tgtgccggca cctgctcgtg gtcgaacgtc
     1861 acgacgtcgc agtgccgggc aaaggcgagg acggcctcga catcggcgtg gtcgcccacc
     1921 ggtgccgacg ggatcaccag cgccgccgag gcgtccggcg cctcggcgag gacgctgagc
     1981 tggacaccga gctcgaccgc cgggccctgg agcatccggg ccagctgccc cccgccgatg
     2041 acgccgacga cggggaaccc gccaggagca cgaaggggcg cagtcacggc gaccgagcct
     2101 atcggctcgg cgcccgagcc agtgcgaggg cgtctgccag gacaccttag gctcgtccgt
     2161 atgacgcagc acgccgggtt ggggggacgg ctgcgcgacg ccttcgagct gatccgtcgc
     2221 gagctcgtcc gcttcggcct cgtcggggcc atggccttcg tcgtcgagat cgcgctgttc
     2281 aacctcttcc tgcacgttct catccgcgac aaggtcacga catcgaagat cgtcgcggcc
     2341 agcgtggcga cgctgtgtgc ctggctcggg aaccgctact ggacgttccg gcagcgctcc
     2401 accggctcgc tccggcgcga gctcgtgctg ttcttcgcgg tcaacgggct ggccatcgcc
     2461 gtcggggcgg cgtggctcgc gttcacccac tacgtgctcg gcctcgacag cagactgcag
     2521 gacaacatca gcaacgtctt cgggatcggc ctggggacgc tgttgcgctt ctgggcctac
     2581 cacgagctcg tcttcgccga ggtcagcgag actcagcctc tgacaggaac aacgcgaact
     2641 tagccgggcg cgccgagatc aggtcgagcc ggccaccgtt ggactcggcc aggtcccggg
     2701 ccagggcgag cccgagcccc gtactcttgc cctgacccgt gctgaccgag cgctggaaga
     2761 tgtgtgcagc gatcgtcgcg gcgatcccgt cgccgtggtc actgacctcg acgacgaccg
     2821 acggcccgct gcggcgggcg cggacatcga ccgtcccgag gccgtggacc agggagttct
     2881 cgagcagggt gctcaggacc tggctcagcg ccccgggcat cgcgcgcacg acgaggccgc
     2941 gttcgccgtc gaccctgatg ctgcgccggg cgttctcgaa ggccggctgc cactcgcgct
     3001 gcagggaggc gatgaccgag tcgagcgaga cctccggcaa cgggccgcca ttggctcgcc
     3061 gtgagcgcat gagcaggtcg tcaacgaccc gggtgagccg ctcgacctgg cccatggcga
     3121 tgcctgcctc ctcatggacg acgtcgaggt cgtccgtcgc agcaacctcc tcgaggcgca
     3181 tgagcagcgc ggtgagcggg gtccgcagct ggtgcgaggc gtccgcggcg aagtcacgct
     3241 cggcggccag cgaggcgcgg acatgttggc cgctgcggac gaggacgtcc gagacgcggt
     3301 cgacctcggg aatgcccgag cgcatcggct cgaccttgga gcggccctca ccgagctgct
     3361 ctgccgaggt gaccaggccc gccatggcgt cggagaagcg ccgcgtcgtc cgataggtga
     3421 gtaacgcggt cagcccgagg gtgagtgcga aggaggcgaa cacctcggca ctgagctgcc
     3481 cgagccggac cgcggcacgc tggtccagcc agtcggtgac gatgtccggg cgggcggcag
     3541 cgacccacag gacggcggcc gtgaccgggc ccatgacgac ggtgaaggcg accgcgacag
     3601 ccgccagagc ggcccgcgcg agctgccggc gcacggcgcc tcagccccgg tccaggcgct
     3661 cgaaacggaa accgaccccg cgcaccgttg cgatgtacct cggctgggcg gcgtcgtcgc
     3721 cgagcttctt gcgcagcacc gagatgtgca tgtccagcgt cttggtcgac ccgaaccagg
     3781 cggtctccca gatctcgcgc atgagctgct cgcgcgagac gaccttgcct tgctcgcgca
     3841 cgagcacccg caggaggtcg aactccttgg cggtgaggac gagctcctcg tcgtggaacc
     3901 acgctcgtcg gccgtcctgg tcgatccgga cgagctcggc gcccgagtcc tcgaccgggc
     3961 tgcgccgcag cagggcccgg actcgagcga gcagctcggc cagccggaag ggcttggtca
     4021 cgtagtcgtc ggcgcccgca tcgagcccga cgacggtgtc gacctcgtcg gcccgggcgg
     4081 tgaggaccag gacggggacc gtgatgccct cggcccggat gcgacggcat acttcgagcc
     4141 cgtcgaggtg gggcaaaccg aggtcgagaa cgagcaggtc cgggttgttg cgggctccgg
     4201 cgagggccga cgggccgtcg gcccggacgt cgacctcgta cccttcgcgg cgcagtgcgc
     4261 gggcgagcgg ctcactgatc gcaggatcgt cctcggccag cagcactcgg gtcatacccg
     4321 aatcgtaggc gtgccgtcat tcgccgggcc agcgagggtc tcgcttctcg gcaaacgcgg
     4381 cgaccccttc ggcgcgatcg cccgagaacg ccgtggcccg ccagcaggaa tcctcgacct
     4441 cgagaccggt ggccaggtcg acatcgaagc cgagccgcat cgcccgtttg gcctgacgca
     4501 gccccaccgg cgagttgcgg gcgatctcgg ccgcgatctc cagcgcccgg tcgcgggccg
     4561 tcccggcggg gacgacctca tcgac
//
LOCUS       DATAIX010000024         1760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_179381, whole genome shotgun sequence.
ACCESSION   DATAIX010000024 DATAIX010000000
VERSION     DATAIX010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179381"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VHM65_00415"
     CDS             <1..572
                     /locus_tag="VHM65_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704197.1"
                     /translation="LALMTDAAIGSPPAHPDQAGAPWRVAAEQWIVDHGPILLAHPWL
                     AFTSAALTTLGPNHLAWAEAIVAALSGTELTPAEKLQVGALLGTHLLNMVRVADGIAA
                     FGHAMTDEPAADRTAGTDYLTLMAALVDPATHPALHAAVRSQPQHAGSAGVAPVQLVF
                     DQWPLDFGARMILDGIGALVADRTGTPPA"
     gene            complement(605..>1760)
                     /locus_tag="VHM65_00420"
     CDS             complement(605..>1760)
                     /locus_tag="VHM65_00420"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HEX2704198.1"
                     /translation="SMTVFDLDSAQTRTVLEADPGVVLRSAPPVLVDEWQRVPEVWDV
                     IRRAVDAGAAPGSFLLTGSATPRRDVVTHSGAGRIVSLRMRPMGLSERDVATPTVSLS
                     ALLDGAGSGLDGSSALTLPDYVEEIERSGYPGIRSLPPRARRAQLDGYLTRVLSRDLD
                     EQGITVRRPASLRAWLEAYAAATSTCASYNQIARAATPGDGDPPAKVTAIRYRDWLSQ
                     LWLLDPVPAWHPRLSRLSALAQAPKHHLADPALAMRLLRASESTLLTGGGRTLGPAGT
                     PLLGALFEDLAALTVRVLAQACEAGTSHLRTQGGEHEIDLIVERHDGRVLAIEVKLAG
                     AVRDQDARHLHWFKNLLGDRLADLLILNTGHHAYRRRDGVAVVPLALLGP"
BASE COUNT          264 a          643 c          606 g          247 t
ORIGIN      
        1 tcctagcact catgaccgat gcggcgatcg gttcgccccc cgcccacccc gaccaggccg
       61 gcgcgccgtg gcgggtcgcc gccgagcagt ggatcgtcga ccatggcccg atcctgctcg
      121 cgcacccctg gctggccttc acctccgccg ccctgaccac gctcgggccg aaccacctag
      181 cctgggcaga ggccatcgtc gccgctctct ccggcaccga gctcaccccg gccgagaagc
      241 tgcaggtggg ggcgctgctc ggcacgcatc tgctgaacat ggtccgggtc gcggacggga
      301 tcgcagcgtt cggtcacgcc atgaccgacg aaccagcggc cgaccgcacc gccggaaccg
      361 actacctgac gctgatggct gcgctcgtcg accccgcgac acatccagcg ctgcacgcag
      421 cggtccgttc gcagccgcag cacgctgggt cggccggtgt cgcaccggtc cagctcgtct
      481 tcgaccagtg gccactcgac ttcggtgccc gcatgatcct cgacggcatc ggcgccctgg
      541 tcgcggaccg aacaggtacg ccccccgcct gaacccacca cgaggagcgc ctgcgcctct
      601 ccgttcaggg accgagcaga gccaacggga caacggccac gccgtcgcgg cggcgatagg
      661 cgtggtgccc agtgttgagg atcagcaggt cggccagacg gtcacccagc aggttcttga
      721 accagtgcag gtgacgggca tcctggtctc ggacggcgcc cgcgagcttg acctcgatcg
      781 ccagtacccg gccatcgtgg cgctccacga tgaggtcgat ctcgtgctca cccccctggg
      841 tgcgcaggtg cgaggttccg gcttcgcacg cctgggccag gactcgcacc gtcaacgccg
      901 cgaggtcctc gaacagggcc ccgagcagcg gggttccggc cgggccgagg gtgcgtccgc
      961 cgccagttag gagtgtggac tcgctggctc gaagcagtcg cattgccagc gccgggtctg
     1021 ccaagtggtg cttcggcgcc tgcgccaggg ccgagagccg gctcagccgt gggtgccagg
     1081 ctggcacggg gtcgagcagc cagagctggg acagccagtc tcggtagcgg atcgcagtca
     1141 ccttcgcggg agggtcgccg tctccggggg ttgccgcacg agcgatctgg ttgtagctcg
     1201 cgcacgtcga ggtcgcggcg gcgtacgcct ccagccacgc gcgcaggctc gccggtcgcc
     1261 ggactgtgat gccttgctcg tcgagatcgc gcgacagtac tcgggtgaga tagccgtcga
     1321 gctgggcccg gcgggcccgc ggcggcagcg accggatccc ggggtatccg gaccgttcga
     1381 tctcctcgac atagtccggc agggtcaggg cgcttgaccc gtcgagacct gaccccgccc
     1441 cgtccagcag cgccgacagg ctcaccgtcg gggtcgccac atcacgctcg ctcagaccca
     1501 tcgggcgcat ccgcagcgag acgatccgcc cggccccgga gtgggtcacc acgtcgcggc
     1561 gaggcgtcgc actgccggtg agcagaaagc tcccgggggc cgccccggcg tccacggccc
     1621 ggcgaatgac gtcccagacc tctggaacgc gctgccactc gtcgacgaga acgggcggag
     1681 cggagcggag aacaaccccg ggatcggctt cgagcacggt ccgcgtctgc gccgagtcga
     1741 ggtcgaagac ggtcatggac
//
LOCUS       DATAIX010000025         3670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_90888, whole genome shotgun sequence.
ACCESSION   DATAIX010000025 DATAIX010000000
VERSION     DATAIX010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90888"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..538)
                     /locus_tag="VHM65_00425"
     CDS             complement(11..538)
                     /locus_tag="VHM65_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEX2704199.1"
                     /translation="MSTDYDAAPTTYRAKRKRRTAITLGFAIAVLAGAFYYGYTYWTA
                     GTSASTACPTGGVTRTIPAGQVAPSSVTVNVYNTTAKAGLAARTGDALRAVGFKIGKV
                     SNDPLRRTVAKIAEIRYGAKGAKQAKTVLGFVPGSTLVKDKRADASVDLAVGQGFVAV
                     KVPAQTPQGGASPSC"
     gene            complement(578..874)
                     /locus_tag="VHM65_00430"
     CDS             complement(578..874)
                     /locus_tag="VHM65_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016997782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEX2704200.1"
                     /translation="MIFKAIGDSRPYPDHGRESVRDWTDVAPRQVRLQELITTKRTLD
                     LAALLSEDSTFYGDLFAHVVQWQGELYLEDGLHRALRAALQQRPTLHARVLVLD"
     gene            947..2557
                     /locus_tag="VHM65_00435"
     CDS             947..2557
                     /locus_tag="VHM65_00435"
                     /inference="COORDINATES: protein motif:HMM:NF017669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX2704201.1"
                     /translation="MTDAPTPAAAAPSVPGGGTEGQLEAIRAGYAFAGPAIDLGAAVI
                     NLQAHPDIPVTIPLGVFNRHGLVAGATGTGKTKTLQLMAEQLSQGGVPVFLADIKGDL
                     SGMATAGVTSDRVTSRAADVGQQWRGTAYPVEFYSLGGQGTGIPMRASVTSFGPTLLA
                     KVLGLNETQESSLGLVFHYADAQGLALLDLKDLRAVISHLTSDEGKAELKSLGGLSSA
                     TAGVILRELIAFSDQGADVFFGEPELDTRDLLRVGADGRGVVSMLELPAVQDRPALFS
                     TFLMWLLADLFHDLPEVGDVDKPKLVFFFDEAHLLFRDASKDFVGSIEQTVRLIRSKG
                     VGVFFVTQSPRDVPSGVLAQLGNRVQHALRAFTPDDAKALKAAVSTYPHSEYDLQQLL
                     TQLGTGEAIITVLSERGAPTPVAWTRMRAPQSLMAPSSPEAVDAVVAASPNTSRYAAA
                     VDRESAYELLAARLAAVPVQDAPAPAPAAERAPAPAPRPARPEKEEPGVIGQVVQSSA
                     FRSMLRSAGTVIGREITRSLFGTARRGR"
     gene            complement(2583..>3670)
                     /locus_tag="VHM65_00440"
     CDS             complement(2583..>3670)
                     /locus_tag="VHM65_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEX2704202.1"
                     /translation="THPLTELDVAGHEMTHGVTESSANLVYSGESGGLNEATSDIFGT
                     AVEWYANNAVDAPDYFIGETININGNGTPLRYLDKPSNDGNSADCWSSTLGGLNVHYS
                     SGPLNHWYYLTSEGSGAKTINGVAYNSPTCNGGTVTGIGHLKAEKIWYRTLVTYLTSG
                     SNYQAARNGAIRSAKDLYGAESAECAAVAGAFSAIAVVPGAETCAAAPPPGTNLLTNG
                     GFEAGATSWAGTSGVITSSTSKPAHSRSWKAWLGGNGRRTTENVKQAVSIPATAASAT
                     LTFWVRIDTAETGSTVYDTLKVQIVSGTTTTLATYSNVGATATYAQKTLSLSAYKGKT
                     VTLKLLASEDSSLQTSFVVDDVVASAG"
BASE COUNT          483 a         1287 c         1297 g          603 t
ORIGIN      
        1 aggtcgcggc tcagcaggag gggctcgcgc cgccctgcgg cgtctgcgcc gggaccttga
       61 ccgcgacgaa gccctgcccg accgcgaggt cgaccgaggc gtcggcccgc ttgtccttga
      121 ccagggtcga tccagggacg aagcccagca ccgtcttggc ctgctttgcg cccttcgcgc
      181 cgtacctgat ctcggcgatc ttggccacgg tgcgcctcag cgggtcgttg ctgaccttgc
      241 cgatcttgaa gcccacggcc ctgagcgcgt cgccggtgcg ggccgccagc cccgccttcg
      301 ccgtcgtgtt gtagacattg accgtgaccg acgacggagc cacctggccg gccgggatcg
      361 tccgggtgac gccgcccgtc ggacaggccg tcgacgcgct cgtgcccgcg gtccagtagg
      421 tgtagccgta gtagaacgcg cccgccagca ccgcgatcgc gaaaccgagt gtgattgccg
      481 tgcgacgctt gcgcttggcg cggtatgtcg tcggcgcggc gtcgtagtcg gtgctcacgg
      541 tggcccttcc gcaggatcga cgggaccttg tcggttgtca gtcaagcacg aggacgcgcg
      601 cgtgcaacgt aggacgctgc tgcaacgcgg cacgaagggc ccgatgcagc ccgtcctcga
      661 ggtagagctc gccctgccac tgcacgacgt gggcgaacag gtcgccgtag aacgtcgagt
      721 cttcggagag cagtgcggcg aggtcgaggg tgcgcttggt cgtgatgagc tcctgcagcc
      781 tgacctgcct gggcgccacg tcggtccagt cccgcacgct ctctctcccg tggtccgggt
      841 aggggcgcga gtcgccgatg gccttgaaga tcacgagccg agtgtatggc gtgcgctcgc
      901 ccgggcgtcc tgccgtcacg gcgtgcgcgc ggctacagtt cgacccgtga ctgatgcacc
      961 cacacccgcc gcggctgctc cctccgtgcc cggcggtggc accgagggcc agctcgaggc
     1021 catccgggcc ggctacgcgt tcgccggtcc cgcgatcgac ctcggcgctg ccgtcatcaa
     1081 cctgcaggcc catccggaca tcccggtcac gatcccgctc ggcgtcttca accggcatgg
     1141 cctcgtcgcg ggggcgacgg gaacgggcaa gaccaagacc cttcagctca tggccgagca
     1201 gctctcgcag gggggtgtgc ccgtcttcct cgccgacatc aagggcgacc tgtccgggat
     1261 ggcgaccgcc ggcgtgacca gcgaccgggt gaccagccgg gcagccgacg tggggcagca
     1321 gtggcgcggc acggcatacc ccgtggagtt ctactccctc ggcgggcagg gcacaggcat
     1381 cccgatgcgc gcatcggtga cgtcgttcgg cccgacactg ctcgcaaaag tgctggggct
     1441 caacgagact caggagtcga gcctcgggct ggtcttccac tacgccgacg cgcagggcct
     1501 ggctctgctc gacctcaagg acctgcgcgc ggtcatctcg cacctcacct cggacgaggg
     1561 caaggccgag ctcaagtccc tcggcgggct gtcctcggcg accgcgggcg tcatcctgcg
     1621 cgagctcatc gccttctccg accagggggc ggacgtcttc ttcggggagc ccgagctcga
     1681 cacgcgcgac ctgctccggg tcggcgccga cgggcgcgga gtcgtgtcga tgctcgagct
     1741 gcccgcggtc caggaccgcc cggcgctgtt ctcgacgttc ctcatgtggc tgctcgccga
     1801 cctcttccac gacctgcccg aggtcggaga cgtcgacaag cccaagctcg tcttcttctt
     1861 cgacgaggcg cacctgctct tccgcgacgc gtcgaaggac ttcgtcggct cgatcgagca
     1921 gaccgtgcgc ctcatccggt ccaagggggt tggcgtgttc ttcgtgacgc agagcccccg
     1981 cgacgtgccg tcgggcgtgc tcgcccagct cggcaaccgg gtccagcacg cgctgcgcgc
     2041 cttcaccccc gacgacgcca aggcgctcaa ggcggcggtc tcgacctacc cgcacagcga
     2101 gtacgacctg cagcagctgc tgacccagct cggcaccggc gaggccatca tcaccgtcct
     2161 gtccgagcgc ggcgcaccga cgccggtggc gtggacgcgg atgcgcgcgc cgcagtcgct
     2221 catggccccc tcgtcaccgg aggcggtcga cgcggtggtc gccgcctcac cgaacacgag
     2281 ccggtatgcc gccgcggtcg accgtgagtc ggcgtacgag ctgctcgccg cgcgcctggc
     2341 ggccgtgccc gtccaggatg ccccggcacc cgccccggcg gctgagcgcg cgcccgctcc
     2401 ggcgccacgc cccgctcgcc cggagaagga ggagccgggg gtcatcggcc aggtcgtcca
     2461 gtcctcggcc ttccggtcga tgctgcgctc ggccggcacc gtgatcggcc gggagatcac
     2521 ccgcagcctc ttcggcacgg cgcgccgggg ccgctgagcg acagcggagc cgggtgcctc
     2581 gctcagccgg cgctcgccac gacgtcgtcg acgacgaagc tcgtctgcag cgaggagtcc
     2641 tcactggcga ggagcttgag cgtgaccgtc ttgcccttgt aggcgctcag gctgagcgtc
     2701 ttctgggcgt acgtcgccgt ggccccgaca ttggagtagg tcgcgagcgt ggtcgtcgtc
     2761 ccgctcacga tctggacctt gagcgtgtcg tagaccgtgc tcccggtctc ggccgtgtcg
     2821 atccggaccc agaaggtgag cgtggccgag gctgcggtgg ccgggatcga cactgcctgc
     2881 ttgacgttct cggtcgtgcg gcggccgttc ccgcccagcc aggccttcca cgagcgcgag
     2941 tgggccggct tggacgtgct ggaggtgatg accccggagg tcccggccca gctcgtggcc
     3001 ccggcctcga agccaccgtt ggtgagcagg ttcgtgccag gcgggggcgc cgccgcgcag
     3061 gtctcggcgc cgggtacgac ggcgatggcg ctgaaggcgc cggcgaccgc ggcgcactcg
     3121 gcgctctccg cgccatacag gtccttggcc gagcggatcg cgccgttgcg cgcggcctgg
     3181 tagttgctcc ccgacgtcag gtaggtgacc agcgtgcggt accagatctt ctcggccttc
     3241 aggtggccga tgccggtcac ggtcccgccg ttgcaggtcg ggctgttgta ggccacaccg
     3301 ttgatggtct tggcgcccga gccctccgag gtgaggtagt accaatggtt gagcgggccc
     3361 gaggagtagt ggacgttgag accgcccagc gtggaggacc agcagtcggc gctgttgccg
     3421 tcgttgctcg gcttgtccag gtagcgcagt ggggtgccgt tgccgttgat gttgatggtc
     3481 tcgccgatga agtagtcggg ggcgtcgacc gcgttgttgg cgtaccactc gacggcggtg
     3541 ccgaagatgt cgctcgtcgc ctcgttgagc ccgccggact cgcccgagta gacgaggttc
     3601 gccgagctct ccgtgacacc gtgggtcatc tcgtggccgg cgacgtcgag ctcggtgagc
     3661 gggtgcgtgt
//
LOCUS       DATAIX010000026         2731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_90926, whole genome shotgun sequence.
ACCESSION   DATAIX010000026 DATAIX010000000
VERSION     DATAIX010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90926"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VHM65_00445"
     CDS             <1..742
                     /locus_tag="VHM65_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2704203.1"
                     /translation="EATVHGAVAGSLINTGQDCTAATRAYVQRPLYDEFVAGVADLMR
                     TVRLGDPMDPDTDQGPLISRRQQERVAGFVERAVATGAKAVCGGRIPGGVLAAGAYYE
                     PTLVTGADPASEIVREEIFGPVLVAVPFDSDDDALALANDSPYGLAASAWTTSHYRAQ
                     RAMREIAAGCVWVNDHIPIVSEMPHGGFKSSGFGKDMSVYSLQEYTQLKHVMSDNTAV
                     ARKGWHRTIFTDRSARGPASGTQSGDPQ"
     gene            739..2121
                     /locus_tag="VHM65_00450"
     CDS             739..2121
                     /locus_tag="VHM65_00450"
                     /inference="COORDINATES: protein motif:HMM:NF004718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX2704204.1"
                     /translation="MTTADHGVTAGFTSADEAKVRRDDRAHVFHSWSAQALIDPLPIA
                     SALGSHFTDYAGKRYLDFSSQLVNVNIGYQHPKLVAAIQEQAARLCTIQPSFANDARS
                     EAARLIAELAPGLLNTVFFTNGGAEATENAIRMARLHTGRHKILAAYRSYHGATNGAI
                     MMTGDPRRWPSEPGMPGVVHFWGPYLYRSAFHAVDAAQECERALGHLREMLMVEGPAT
                     VAGIILETVVGTNGILVPPNGYLARVRAICDELGIVLIADEVMAGFGRCGEWFAVDRW
                     AVEPDLITFAKGVNSGYVPLGGVLICDTIAGTFEQRAYPGGLTYSGHPLACASAVASI
                     EIFKEEGIIEHARMLGAEVIGPELARMAEKHPSVGDVRGLGVFWALELVKDRATREPL
                     VPYNASGAAAAPMNDFAAACKARGLWPFVHFNRTHVVPPCTTTVEEVREGLAILDEAL
                     DVTDLEYTGS"
     gene            complement(2135..>2731)
                     /locus_tag="VHM65_00455"
     CDS             complement(2135..>2731)
                     /locus_tag="VHM65_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX2704205.1"
                     /translation="DVEGARDSWLADASVLVERWFGLEDPTRIEPAERELYVEAEIEG
                     LTLRGYVDRLDVAPDGALRVVDYKTGRSPSELFETRALFQMKFYALVLWRLRGVVPRM
                     LQLVYLGNSEVVRYAPDEADLLATERKVKALWVAIERAASAGDWRPRPSRLCDWCDHR
                     GICPAWGGTPPPLPQDAERLALDPRRTGLVTVTEATGD"
BASE COUNT          411 a          989 c          943 g          388 t
ORIGIN      
        1 ggaggccacc gtgcatggcg ccgtggccgg gtccctcatc aacacggggc aggactgcac
       61 ggccgccacg cgggcgtatg tccagcgtcc cctgtatgac gagttcgtcg ccggggtggc
      121 cgacctcatg aggacggtgc gcctcggtga cccgatggac ccggacaccg accagggacc
      181 gctgatctcc cggcgccagc aggagcgggt ggcgggcttc gtcgagcggg ccgtggcgac
      241 gggagccaag gcggtctgcg gtgggcgcat ccccggtggc gtgctcgcag ccggtgccta
      301 ctacgagccg accctcgtca ccggggccga ccccgcgtcg gagatcgtgc gcgaggaaat
      361 cttcggcccg gtgctcgtcg cggtgccgtt cgactcggac gacgacgccc tggccctggc
      421 caacgactcg ccgtacgggc tggcggcctc ggcctggacg acgagccact accgggcgca
      481 gcgcgcgatg cgcgagatcg ccgccgggtg cgtgtgggtc aacgaccaca tcccgatcgt
      541 cagcgagatg ccgcacggcg ggttcaagtc gagcggcttc ggcaaggaca tgtcggtgta
      601 ctccttgcag gagtacaccc agctcaagca cgtcatgtcg gacaacaccg cggtggcccg
      661 caagggctgg caccgcacga tcttcaccga ccggtccgca cgcggaccgg catccggcac
      721 gcagtcagga gaccctcagt gaccaccgca gaccatggcg tcaccgccgg attcaccagc
      781 gccgacgagg cgaaggtccg gcgggacgac cgggcccacg tgttccactc gtggtcggcg
      841 caggccctca tcgacccgct gcccatcgcc tcggccctgg ggtcgcactt caccgactat
      901 gcgggcaagc gctacctcga cttctcctcc cagctggtca acgtcaacat cggctaccag
      961 caccccaagc tcgtcgcggc catccaggag caggccgccc ggctgtgcac gatccagccg
     1021 tcgttcgcca acgacgcccg gtcggaggcc gcccggctca tcgccgagct ggccccgggc
     1081 ctgctgaaca cggtgttctt caccaacggc ggcgccgagg ccacggagaa cgccatccgg
     1141 atggcccgcc tgcacacggg gcggcacaag atcctcgcgg cctaccgcag ctaccacggc
     1201 gcaaccaacg gcgcgatcat gatgaccggt gacccacgcc gctggccctc ggagccaggt
     1261 atgccgggcg tcgtccactt ctgggggccg tacctctacc gctcggcatt ccacgcggtc
     1321 gacgcggcgc aggagtgcga gcgcgccctg gggcacctgc gcgagatgct catggtcgag
     1381 ggcccggcga ccgtggcggg gatcatcctc gagaccgtcg tcggcaccaa cgggatcctc
     1441 gttcccccga acggttacct ggccagggtg cgcgcgatct gtgacgagct cgggatcgtc
     1501 ctcatcgcgg acgaggtcat ggccggcttc ggtcgctgcg gggagtggtt cgcggtggac
     1561 cgctgggcgg tcgagcccga cctcatcacc tttgccaagg gcgtcaactc gggctacgtg
     1621 cccctcggcg gggtgctcat ctgcgacacc atcgcgggca ccttcgagca gcgtgcctac
     1681 cccgggggcc tgacctactc gggacacccc ctggcctgtg cctccgcggt cgcctcgatc
     1741 gagatcttca aggaggaggg gatcatcgag cacgcccgga tgctcggcgc cgaggtgatc
     1801 ggccccgagc tggcccggat ggccgagaag cacccgagtg tgggggacgt ccgcggactg
     1861 ggggtcttct gggccctgga gcttgtcaag gaccgggcga cgcgtgagcc gctcgtgcct
     1921 tacaacgcct cgggcgcggc cgccgcgccg atgaacgact ttgccgcggc gtgcaaggcc
     1981 cgcgggctgt ggcccttcgt gcacttcaac cggacccatg tcgtgccccc ctgcacgacc
     2041 acggtcgagg aggtccgcga aggcctggcc atcctcgacg aggccctcga cgtcaccgac
     2101 ctcgagtaca ccggcagctg agcactccat cccgtcagtc gcccgtggcc tcggtcacgg
     2161 tgaccagccc ggtgcggcgc gggtccaggg cgaggcgctc ggcgtcctgt ggcaaggggg
     2221 gaggcgtgcc cccccaggcc gggcagatgc cccggtggtc gcaccagtcg cacagccggg
     2281 agggcctggg ccgccagtcg ccggcggacg ccgcgcgctc gatcgcgacc cagagcgcct
     2341 tgaccttgcg ttcggtggcg agcaggtccg cctcgtcggg tgcgtagcga acgacctcgc
     2401 tgttgccgag atagaccagc tggagcatcc gcgggacgac tccccgcagc cgccacagga
     2461 caagggcgta gaacttcatc tggaacagtg ccctggtctc gaagagctcc gagggcgagc
     2521 gcccggtctt gtagtcgacg acccgcagcg cgccgtctgg cgcgacgtcg agccggtcga
     2581 catacccgcg cagggtcagg ccctcgatct cggcctcgac gtagagctca cgctcggccg
     2641 gctcgatccg ggtcgggtcc tccagcccga accagcgctc gacgagcacc gaggcgtccg
     2701 cgagccagga gtcgcgcgcc ccctcgacgt c
//
LOCUS       DATAIX010000027         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_61176, whole genome shotgun sequence.
ACCESSION   DATAIX010000027 DATAIX010000000
VERSION     DATAIX010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61176"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VHM65_00460"
     CDS             <1..327
                     /locus_tag="VHM65_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704206.1"
                     /translation="GMSDHLEDTRVITEDAPQYNATLVRRVDYSGELASFWVKLDGDP
                     TPFAAGQYMTAGLFADGKLWQRPYSVASAPRVAGSEGYELYVRLVPVIRLRRSSGASR
                     RGRGCG"
     gene            348..824
                     /locus_tag="VHM65_00465"
     CDS             348..824
                     /locus_tag="VHM65_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704207.1"
                     /translation="MLEPDDRRTHLYVSTGTGIAPFLGMIRETQLAGEMRRTVVLHGA
                     SFADELGYRDLLEALEREGTYPLRYVPTVSRPLDPRNAGWAGRIGRVEAVVADVCHDL
                     HLSADRTVVYICGNPDMILNVESVLMGRGFPEFHVKKELYWPKGKQPAGAGAPASG"
     gene            complement(897..1553)
                     /gene="hypB"
                     /locus_tag="VHM65_00470"
     CDS             complement(897..1553)
                     /gene="hypB"
                     /locus_tag="VHM65_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEX2704208.1"
                     /translation="MTDTGRTTRVPLVERVLEANDEIAAANRAELERRGIFSVDLIGA
                     PGSGKTALLEATLRALQGRRRPAVLVGDLATRRDGDRLARWCDKVVQISTGQGCHLEA
                     NQVREAFGQIDLDTVDLLFIENVGNLICPVGFDLGQTTKVGLFSLTEGDDKAAKHPYL
                     VEAADLILLNKIDLLPHLTFDRPSFEADVHLLNPTVTILDVSATTGQGLDAWIAWLDR
                     "
     gene            complement(1555..1899)
                     /locus_tag="VHM65_00475"
     CDS             complement(1555..1899)
                     /locus_tag="VHM65_00475"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEX2704209.1"
                     /translation="MHEFSIAEALATQVLRHAPATGRVRQVEICVGALRGLEPDALRM
                     CWDAVTHDTPIAGAVLQVDLRPWSITCSTCGRTWPSPVPFVACACGDPAPVPTAGDEL
                     NLVALTIDEEET"
BASE COUNT          298 a          688 c          722 g          303 t
ORIGIN      
        1 ggcatgagcg atcatctcga ggacacgcgc gtcatcaccg aggacgcccc gcagtacaac
       61 gcgacgctgg tccgtcgcgt tgactattcg ggcgagctgg caagcttctg ggtgaagctc
      121 gacggcgacc cgaccccgtt cgcggccggg cagtacatga cggccgggct gttcgccgat
      181 ggcaagctct ggcagcgccc ctactcggtg gcgtcagcgc cacgcgttgc cgggagcgag
      241 ggatacgagc tctacgtccg gctcgtgccg gtgatccgtt tacgacgctc ctctggcgcc
      301 tccaggaggg ggcgaggctg cggctgatcg gtccgaaggg tcgcttcatg ctcgagcccg
      361 acgaccgccg gacgcacctg tacgtctcca ccgggaccgg gatcgcgccc tttctcggca
      421 tgatccgcga gacgcagctg gccggggaga tgcgcaggac ggtggtgctc cacggtgcct
      481 ccttcgccga cgagcttggc taccgcgatc tgctcgaggc gctggagcgc gagggcacct
      541 atccgctccg ctacgtgccc acggtgtcgc ggccactgga cccgcggaac gcgggctggg
      601 caggcaggat cggtcgcgtc gaggcggtgg tcgcggacgt ctgccacgac ctgcacctgt
      661 cggccgaccg cacggtcgtc tatatctgcg gcaaccccga catgatcctc aacgtcgaga
      721 gcgtcctgat ggggcgtggc ttccccgagt tccacgtcaa gaaggagctg tactggccga
      781 agggaaagca gcccgccggg gcgggcgcgc ccgcgagcgg ctgaccatcc tcgcgagcct
      841 cgtcgccgcc cgtacccccc cgggggggcc caatcgcgcg gccgcggcac gaccggtcag
      901 cgatcgagcc aggcgatcca ggcgtcgagg ccctggccgg tcgtggccga gacgtcgagg
      961 atcgtgaccg tcggattgag caggtggaca tcggcctcga agctcggccg gtcgaacgtc
     1021 aggtgcggca gcaggtcgat cttgttgagc aggatgaggt cggccgcctc gaccaggtag
     1081 gggtgcttgg ccgccttgtc atccccctcg gtcaggctga acaggccgac cttggtggtc
     1141 tgtccaaggt cgaagcccac cgggcagatc aggttgccga cgttctcgat gaacagcagg
     1201 tcgaccgtat cgaggtcgat ctggccgaag gcctcgcgaa cctggttcgc ctccaggtga
     1261 caaccctgac cggtcgagat ctggaccacc ttgtcgcacc agcgggcgag gcggtccccg
     1321 tcccggcggg tcgccaggtc gccaacgagg acggccggac ggcgacggcc ctggagagcg
     1381 cgcagggtcg cctccaggag ggccgtcttg ccgctccccg gcgccccgat caggtcgacc
     1441 gagaagatgc cccgtcgttc cagctccgcg cgattcgccg cggcgatctc gtcgtttgcc
     1501 tccaggaccc gctcgacgag cggcacccgg gtcgttcgcc cggtgtcggt catgtcaggt
     1561 ctcctcctcg tcgatcgtga gggcaacgag gttgagctcg tcgcccgccg tcggcaccgg
     1621 cgccgggtcg ccacaggcgc aggccacgaa tggcaccggg ctcggccagg tgcgaccgca
     1681 ggtggagcac gtgatggacc acggtcggag atccacctgg aggaccgccc cggcgatggg
     1741 ggtgtcatgg gtcacggcgt cccagcacat ccggagcgcg tccggctcga gcccgcgcaa
     1801 cgcgccgacg cagatctcga cctggcgcac gcggcccgtg gcgggcgcgt ggcgcaggac
     1861 ctgcgtcgcg agggcttcgg cgatcgagaa ctcgtgcacg cggagcatgg tagcgctcgg
     1921 gccgggaagg ccccgaacgg gggctccggg acgactcggc ccgtccatct cgggccgttg
     1981 agccctgtgc cgaagggccg atctctacgg g
//
LOCUS       DATAIX010000028         2706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_119834, whole genome shotgun sequence.
ACCESSION   DATAIX010000028 DATAIX010000000
VERSION     DATAIX010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119834"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1714
                     /gene="glgP"
                     /locus_tag="VHM65_00480"
     CDS             <1..1714
                     /gene="glgP"
                     /locus_tag="VHM65_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX2704210.1"
                     /translation="FQGVERINELMRDFDLTFDEALEGVRAATVFTTHTPVPAGIDRF
                     GADKIHVYFGEHGAVPGVPLERLLALGGEDFEGGSRGVFNMAIMGLRLGQRANGVSEL
                     HGAVSRDMFDGLWPGFDPTEVPITSITNGVHAGTWVDDRVFALAEKHLGTIEVSRDHA
                     WERIGEIPVEDMWVTKRGLREQLVKDARKRVKASWIKRGASAAELGWVEDILDPDVLT
                     IGFARRVPTYKRLTLMLRDPARLKRMLLHPDRPVQVVIAGKSHPADETGKRLIQQMVQ
                     FADDPQVRHRIVFLPNYDIEMAQKLYPGCDVWLNNPLRPFEACGTSGMKAALNGALNL
                     SILDGWWDEWFDGSNGWAIPTADGVENPDRRDDIESAALYDLIENAVAAHFYDRDMDG
                     LPQHWLSMIAHTLATLGPKVLASRMVRDYTEKLYGPAARAGWALAGATYAGARDLAEY
                     KARVRAAWPGVRVDHVEPSGVSDSPQIGDTLEVRAFVDLGGLEPEDVEVQFVHGRTSE
                     NDDLREPEVVPLAFGESHEGDRHAFVGSLKLARTGSFGYTVRVLPKHPALATGAELGL
                     VANA"
     gene            complement(1904..>2706)
                     /locus_tag="VHM65_00485"
     CDS             complement(1904..>2706)
                     /locus_tag="VHM65_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704211.1"
                     /translation="SQDLFGARDRGPVASINFIAAHDGFTIADLTAYNGKHNDANGEG
                     NRDGSNDNRSWNHGIEGPTDDPIVAGLRRRNIRNMLATLLVSTGVPMLCAGDEFGRTQ
                     RGSNNAYCQDNEISWFDWELASWQRDLLDTTRFLVGLRRTHPVLRQRAFFSGRPEHED
                     GSTDLAWYDVDGDPMNNSRWEDPSARTLQMLLDGGPLGTDSLLVVVHGGAHDRDLTLP
                     RPPGITAYELLWDSTWERPGAPALADLDDEPVVVSAASVRIYRAIGPT"
BASE COUNT          399 a          957 c          942 g          408 t
ORIGIN      
        1 cttccagggt gtcgagcgga tcaacgagct catgcgcgac ttcgacctca ccttcgacga
       61 ggcgctcgag ggcgtccgcg cggccacggt gttcaccacc cacacgccgg tccccgcggg
      121 gatcgaccgg ttcggcgccg acaagatcca cgtctacttc ggtgagcacg gcgccgtccc
      181 gggtgtcccg ctcgagcggc tcctggccct tggcggcgag gacttcgaag gcggctcgcg
      241 cggagtcttc aacatggcga tcatggggct gcgcctgggc cagcgcgcca acggcgtctc
      301 cgagctgcat ggtgccgtga gccgtgacat gttcgacggc ctgtggccgg gattcgaccc
      361 gaccgaggtg ccgattacgt ccatcaccaa cggtgtccac gcggggacgt gggtcgacga
      421 ccgtgtcttc gcgctggccg agaagcacct cggcaccatc gaggtctcgc gcgaccacgc
      481 ctgggagcgc atcggcgaga tcccggtcga ggacatgtgg gtcaccaagc gcgggctgcg
      541 cgagcagctc gtcaaggatg cccgcaagcg ggtcaaggcg tcctggatca agcgcggcgc
      601 cagcgccgcc gagctcggct gggtggagga catcctcgac cccgacgtcc tgaccatcgg
      661 cttcgcccgg cgcgtcccga catacaagcg gctcaccctc atgctgcgcg acccggcacg
      721 gctcaagagg atgctcctgc acccggaccg cccggtccag gtcgtcatcg caggcaagtc
      781 ccacccggcc gacgagacgg gcaagcggct catccagcag atggtgcagt tcgccgacga
      841 cccgcaggtc cgtcaccgga tcgtcttcct gcccaactac gacatcgaga tggcccagaa
      901 actctacccg ggctgcgacg tctggctcaa caacccgctg cgtcccttcg aggcgtgcgg
      961 cacatcgggc atgaaggccg cgctcaacgg tgctctcaac ctgtcgatcc tcgacgggtg
     1021 gtgggacgag tggttcgacg gctcgaacgg ctgggcgatc ccgacagccg atggtgtcga
     1081 gaacccggac cgccgcgacg acatcgagtc ggccgcgctc tacgacctca tcgagaatgc
     1141 cgtcgcggcg cacttctacg accgcgacat ggacgggctc ccccagcact ggctctcgat
     1201 gatcgcccac acgctcgcga cgctcggccc caaggtgctc gcctcgcgca tggtgcgcga
     1261 ctacaccgag aagctctacg gtccggccgc ccgcgccggg tgggcgctcg ccggcgccac
     1321 ctacgccggg gcacgcgacc tcgcggaata caaggcccgc gtccgggcag catggccggg
     1381 cgtgcgcgtc gaccacgtcg agccctcggg ggtctcggac tcgccgcaga tcggggacac
     1441 cctcgaggtg cgggcctttg tcgacctggg agggctcgag cccgaggacg tcgaggtgca
     1501 gttcgtccac gggcgcacgt ccgagaacga cgacctgcgt gagcccgagg tcgtgccgct
     1561 ggccttcggc gagagccacg aaggggaccg gcacgcgttc gtcggctcgc tcaagctggc
     1621 gcggaccggc tcgttcggct acaccgtgcg cgtcctgccc aagcacccgg ccctggcgac
     1681 cggggccgag ctgggcctcg tcgccaacgc ctgagcgccc ggtccgcgct gcgcggcgtc
     1741 tcttccggac atcgcgcgtc cgccgacccg gccgcgccgc atgtcgcgac acgcgcacac
     1801 gcctggttgc gcagttgcgc aaccaggttg cgcacccgga gttccacaac cggcctgcgc
     1861 acccgtgttg cggcgtcccg caaccacgcg gagttgggcc gggtcaggtc gggccgatcg
     1921 cacggtagat ccgcaccgag gccgccgaga cgaccaccgg ctcgtcgtcg aggtcggcca
     1981 gcgcaggcgc gccgggtcgc tcccaggtgc tgtcccagag cagctcgtat gcggtgatgc
     2041 cgggtgggcg cgggagcgtc aggtcccggt cgtgagcgcc gccgtggacg acgacgagca
     2101 gcgagtccgt gcccagcggg cccccgtcga ggagcatctg cagggtccgc gccgacgggt
     2161 cctcccatcg gctgttgttc atcgggtcac cgtcgacgtc ataccaggcc aggtcggtgg
     2221 acccgtcctc gtgctccgga cgtccggaga agaacgcccg ttgccgtagc acggggtggg
     2281 tgcgccggag gccgacgagg aagcgcgtcg tgtcgagcag gtcgcgctgc cagctggcga
     2341 gctcccagtc gaaccacgag atctcgttgt cctggcagta ggcgttgttg gagccgcgct
     2401 gggtgcggcc gaactcgtca ccggcgcaga gcatcggcac accggtcgac acgagcaggg
     2461 tcgcgagcat gttgcggatg ttgcggcgcc ggagcccggc cactatcggg tcgtcggtcg
     2521 gcccctcgat cccgtggttc cacgagcggt tgtcgttgct gccgtcccgg ttgccctcgc
     2581 cgttggcgtc gttgtgcttg ccgttgtagg ccgtgaggtc ggcaatcgtg aacccgtcat
     2641 gggcggcgat gaagttgatc gaggccaccg gcccgcggtc ccgcgccccg aagaggtcct
     2701 gtgagc
//
LOCUS       DATAIX010000029         2884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_150742, whole genome shotgun sequence.
ACCESSION   DATAIX010000029 DATAIX010000000
VERSION     DATAIX010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150742"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..657)
                     /locus_tag="VHM65_00490"
     CDS             complement(127..657)
                     /locus_tag="VHM65_00490"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704212.1"
                     /translation="MTWWRLEDLPDDLMELAATLDPNPTYVVGARWDVLASNPAAHRL
                     FANWPALPRRQRNLLWYYCVDPSARELFVDWEQEAADQLALFREAYKRRAGDPSYARL
                     LERIFEANPAAQDWFDRHQASTKRSGDKRIRLETGQVLGLRQLVLHHADDPDIKVITY
                     FADVDFDDDLWEELDS"
     gene            complement(694..2181)
                     /locus_tag="VHM65_00495"
     CDS             complement(694..2181)
                     /locus_tag="VHM65_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEX2704213.1"
                     /translation="MSAPTDGGRPAYGGSMTTTASTTSGLALETNGINVISEDERKGS
                     PRDLFWPWAAANVSVFGLSYGAYFLGFGISAAQALVAGLLGTVLSFLLVGFVSLAGKR
                     GSAPTMVLSRASFGVRGNALPAALSYLLLVGWEIILVALATLATATVAERLGWGSGNL
                     LKVVAFLVVIAVIMAGGMLGFDVIMRLQTWITVATVILTVGYMVLTVGRIDWGAVGAV
                     PSGSVQGMLGALILGITAFGLGWTNSGADYSRYLPRTTPGAGVVGWTTFGAAVAPVLL
                     VLFGVPLAASDAELSAAIGSDPIGALTTILPTWYLIPFALVAILGLIGGAVLDIYSSG
                     LALLTLGLKVPRWVAAGVDGVIAILGTTYVVWVAEDFFGPFMAFLITLGVPVAVWCGV
                     FLADLLLRRRPYDDAALYESGGRYGSVNWVSWALVGVGTVLGWGLVTNTTKGFGWLGY
                     LLGPLGGREGAWAYANLGVLVAIAIGFAGYSVLCSGRVRRQESSS"
     gene            2318..>2884
                     /locus_tag="VHM65_00500"
     CDS             2318..>2884
                     /locus_tag="VHM65_00500"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEX2704214.1"
                     /translation="MTVGASAGQRDRARGALVGLAVGDALGMPAQMLTRDRVAGLWGR
                     IRGFETGPAESEIAPLMPAGRVTDDTDQAVILAELLLAGGGYVDPEAFARRLLDWESH
                     LVARGSLDLLGPSTKRAVEAFRAGTAVTATGRWGDTNGAAMRVAPVGVLMPADPVAAL
                     AERVAHVNEVTHNTGVANAGASAVATPVL"
BASE COUNT          460 a          977 c         1088 g          359 t
ORIGIN      
        1 gcgcgggaga aggtcaatgc tctttgcgta agcgcgactg tcgattggtc gagtcttgtc
       61 catgagcatg cgtttgacca ccgagaggtg cggatacacc tggacgctgg ggcttgatag
      121 gcgcggtcag gagtcgagct cctcccacag gtcgtcgtcg aagtcgacgt cggcgaagta
      181 ggtgatgacc ttgatgtcgg ggtcgtcggc atggtgcagc acgagctggc gcagccccag
      241 gacctggccg gtctcgagcc ggatccgctt gtccccgctg cgtttggtcg aggcctggtg
      301 gcggtcgaac cagtcctgag ctgccgggtt ggcctcgaag atgcgctcca ggagccgggc
      361 gtatgacggg tcgccggccc gccgcttgta ggcctcgcgg aagagcgcga gctggtctgc
      421 ggcctcctgc tcccagtcga cgaagagctc ccgggccgag gggtcgacgc agtagtacca
      481 gagcaggttg cgctggcgcc ggggcagggc cggccagttg gcgaagagcc gatgtgcggc
      541 cgggttcgac gcgaggacgt cccagcgagc cccgacgaca tacgtcgggt tggggtccag
      601 agtcgcggcc agctccatga ggtcgtcagg gaggtcctcg aggcgccacc acgtcatacg
      661 cctatcctgc caggggccgg cggcggcacc ggctcagctg ctcgactcct gacggcggac
      721 gcggccgctg cagagcacgg agtagccggc gaacccgatg gcgatcgcga cgaggacgcc
      781 gaggttcgcg taggcccagg ccccctcacg gccgccgagc gggcccagga gatagccgag
      841 ccagccgaac cccttggtcg tgttggtcac caggccccag ccgaggacgg tgcccacgcc
      901 gacgagggcc caggagaccc agttcaccga gccgtagcgc ccgcccgact cgtagagggc
      961 cgcgtcgtca tacgggcgcc tgcgcagcag caggtcggcg aggaacaccc cgcaccacac
     1021 cgcgacgggc acgcccaggg tgatgaggaa cgccatgaac gggccgaaga agtcctcggc
     1081 cacccagacg acgtaggtcg tgccgaggat cgcgatgacg ccgtcgacac cggcggccac
     1141 ccagcgcggc accttgaggc cgagggtgag cagggccagc ccggaggagt agatgtcgag
     1201 gacggctccg ccgatgagcc cgaggatcgc gacgagcgcg aaggggatga ggtaccacgt
     1261 cggcaggatc gtcgtcaggg caccgatggg gtcggacccg atcgccgccg agagctcggc
     1321 gtccgacgcg gcgaggggga cgccgaagag cacgagcagc accggcgcca cggccgcgcc
     1381 gaacgtggtc cagccgacca cgccggctcc cggggtggtg cggggcaggt agcgggagta
     1441 gtccgcgccc gagttggtcc agccgaggcc gaacgccgtg atgccgagga tcagggcacc
     1501 gagcatgccc tggacggacc cggacgggac cgccccgacc gcgccccagt cgatcctgcc
     1561 tacggtgagg accatgtaac ccaccgtgag gatgacggtc gccacggtga tccacgtctg
     1621 gagcctcatg atgacgtcga agccgagcat gccaccggcc atgatgacgg cgatgacgac
     1681 gaggaacgcg acgaccttga gcaggttgcc cgaaccccag ccgaggcgct cggccaccgt
     1741 ggccgtcgcg agggtcgcca gggcgacgag gatgatctcc cagccgacga ggagcaggta
     1801 ggacagcgcc gccggcagtg cgttgccgcg gaccccgaag gacgcgcggc tcagcaccat
     1861 cgtcggcgcc gagccacgct tgccggcgag cgagacgaac ccgacgagca ggaacgacag
     1921 cacggtcccg agcaggccgg ccaccagggc ctgcgcggcc gagatcccga acccgaggaa
     1981 gtaggcccca tagctcaggc cgaagaccga cacgttcgcc gccgcccacg gccagaacag
     2041 gtcgcgcggg ctgcccttgc gctcgtcctc gctgatgacg ttgatgccgt tggtctccag
     2101 ggcaagaccg ctcgtggtcg aggccgtggt cgtcatactt cctccgtatg ccgggcgccc
     2161 accgtccgtg ggtgcactca ccccccaatg gggttcagca cgccgatccg atgatccgtc
     2221 ggtccgaggg tagcggcgcg gagacgccct cgacgagccg gtcgtcggcg gcgccggcgg
     2281 gctgcccgct cccgggggca gatggcaggc tggccccatg acggtgggcg cgagcgcggg
     2341 gcagcgggac cgcgcgcggg gagcgctcgt cgggctggcc gtcggcgacg cgctcgggat
     2401 gccggcccag atgctgaccc gggaccgggt ggccgggctg tggggacgga tccggggctt
     2461 cgagaccggc ccggccgaga gcgagatcgc gccgctcatg cccgcggggc gggtcaccga
     2521 cgacaccgac caggccgtga tcctcgccga gctgctcctc gccgggggtg ggtacgtcga
     2581 cccggaggcc ttcgcgcgcc ggctgctcga ctgggagagc cacctcgtgg cccgcgggtc
     2641 gctcgacctg ctcgggccct cgacgaagcg ggcggtcgag gcgttccgcg ccgggactgc
     2701 ggtcacggcg accgggcgct ggggggacac caacggcgcg gcgatgcggg tcgccccggt
     2761 cggggtcctc atgccggccg acccggtggc ggcgttggcc gagcgggtcg cccacgtcaa
     2821 cgaggtcacc cacaacacgg gcgtggccaa cgcgggggcc tcggcggtgg ccacgcccgt
     2881 gttg
//
LOCUS       DATAIX010000030         1913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_209289, whole genome shotgun sequence.
ACCESSION   DATAIX010000030 DATAIX010000000
VERSION     DATAIX010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209289"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VHM65_00505"
     CDS             complement(<1..989)
                     /locus_tag="VHM65_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704215.1"
                     /translation="MAIADDVARLHDPATYVEGVPYEVLARLRRERGVVRVPEPGLPG
                     WPPGPGYWLVLRHTDIERVLRDPGTFSSAVGGTQMRDPGTAEDLAYVRRMMLNMDPPE
                     HTRLRRMLATSFTPRAVAALEGQIGGHARGIVERMLDGQRSGECDFAKDVGADLPLLT
                     LADLLGVPGQDRWLMFDWSNRVIGWQDPDYSTSATFDAASGSAMARQAGALRPEPGPD
                     GRMPDPRSRAGMPDLYRYAHLLGQAKREHPGDDVMSTLMAQRDDSASGDGHVSLEEFE
                     NLFWLFAVAGNETLRNGIPGGMLALLAHPPAQRALRTDPARVAAAVEEMLRWWT"
     gene            complement(999..1754)
                     /locus_tag="VHM65_00510"
     CDS             complement(999..1754)
                     /locus_tag="VHM65_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2704216.1"
                     /translation="MSIAHRTVGSGPHTVLCLPGWFGSSTGWGSFPSYVDGEAFTYVF
                     VDYRGYGARRDEAGDYTLEEIAADVLALADELGVERFSLIGHSMGGSAVQRVLSKAPE
                     RVVKMVGIAPVGASPMPFDEAGHALFYGAAESRDNRYGIIDFTTGNRNTSVWVNQVVE
                     HSLAGSTVDSFAGHLVAWATADFQDEVMGMALPVLAVVGEHDPALGEATIRATWVPTY
                     PNCEVAVMANAGHYPMDETPVQLATVIERFLTT"
BASE COUNT          286 a          701 c          659 g          267 t
ORIGIN      
        1 gtccaccagc gcagcatctc ctcgacggcg gccgcgaccc gcgccgggtc cgtacggagc
       61 gcccgctgag ccggcggatg cgcgagcagc gcgagcatcc cgcccgggat gccgttgcgc
      121 agcgtctcgt tgcctgccac cgcgaacagc cagaacaggt tctcgaactc ctcgaggctg
      181 acatgcccgt cgccgctggc cgagtcgtca cgctgtgcca tgagggtcga catgacgtca
      241 tcgccgggat gctcgcgctt ggcctgcccg agcaggtgcg cgtaccggta gaggtccggc
      301 atacccgccc ggctgcgggg gtccggcatc cggccgtcgg gcccgggctc cggccgcagc
      361 gcacccgcct gtcgcgccat tgccgagcct gaggcggcgt caaaggtggc gctcgtcgag
      421 tagtccgggt cctgccaccc gatgacgcgg ttggaccagt cgaacatgag ccatcggtcc
      481 tgcccgggga ctcccagcag gtcggccagc gtgagcagcg gcaggtccgc cccgacgtcc
      541 ttggcgaagt cacactcgcc tgaccgctgc ccgtcgagca tccgctcgac gatcccccgc
      601 gcgtgcccgc cgatctggcc ctcgagcgca gccaccgccc gcggcgtgaa ggacgtcgcg
      661 agcatccgcc gcagccgggt gtgctccggc gggtccatgt tgagcatcat ccggcggacg
      721 taggcgaggt cctccgccgt gcccgggtcc cgcatctggg tgccgcccac ggccgaggag
      781 aacgtccccg ggtcgcgtag cacccgctcg atgtcggtgt gccgcagcac cagccagtag
      841 cccgggcccg gcggccagcc cggcagcccc ggctcgggca cgcggaccac gccgcgctcg
      901 cggcgaaggc gcgcgagcac ctcgtacggg acaccctcga cgtacgtcgc cgggtcgtgc
      961 agacgagcga cgtcgtcggc gatggccacg ggcgccggtc aggtcgtgag aaagcgctcg
     1021 atgaccgtcg cgagctgcac cggagtctcg tccatcgggt agtggcccgc gttggccatc
     1081 accgcgacct cacagttcgg gtatgtcggc acccaggtcg cccggatcgt cgcctcgccc
     1141 agggccgggt cgtgctcgcc gacgaccgcg aggacgggaa gggccatgcc catgacctcg
     1201 tcctggaagt ccgcggtcgc ccacgcgacg agatgcccgg cgaacgagtc gacggtcgag
     1261 ccggccaggg agtgctcgac gacctggttg acccacacgc tcgtgttccg gttgcccgtg
     1321 gtgaagtcga tgatcccgta ccggttgtcg cggctctcgg ccgcgccgta gaagagggca
     1381 tgaccggcct cgtcgaacgg catcggagac gcccccaccg gcgcgatgcc aaccatcttc
     1441 acgacgcgct ccggagcctt ggacagcacc cgctggacgg cgctaccgcc catcgagtgc
     1501 ccgatgaggc tgaaccgctc gaccccgagc tcgtcggcga gcgcgagcac atcggcggcg
     1561 atctcctcga gggtatagtc ccccgcctcg tccctgcgcg cgccataccc gcggtagtcg
     1621 acgaagacgt acgtgaacgc ctccccgtca acgtatgacg ggaagctccc ccatccggtc
     1681 gaggagccga accagcccgg gaggcacaag acggtgtgcg gaccagagcc aacagtgcgg
     1741 tgcgcgatcg acatgtgtct ctctccttga cgggcgatcg attcctggtg ccacgtccga
     1801 gcctaagggt gacccgagat ccgtgcatcg tcggcggcgg ccctgagagc gccggtattg
     1861 tctggcgcaa catccgggga gcccccgggg agcccccggg gagccccgag agg
//
LOCUS       DATAIX010000031         2676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_179613, whole genome shotgun sequence.
ACCESSION   DATAIX010000031 DATAIX010000000
VERSION     DATAIX010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179613"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..318)
                     /gene="secE"
                     /locus_tag="VHM65_00515"
     CDS             complement(46..318)
                     /gene="secE"
                     /locus_tag="VHM65_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX2704217.1"
                     /translation="MAETSAATRDSGRVSRKRGQSGNPVSRFLRSMSLFVSQIIDELR
                     KVVRPTRSELVNYTTVVILFVLVIMAIVAGLDLLFQKLVLLAFAKN"
     gene            complement(406..478)
                     /locus_tag="VHM65_00520"
     tRNA            complement(406..478)
                     /locus_tag="VHM65_00520"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(443..445),aa:Trp,seq:cca)
     gene            651..1880
                     /locus_tag="VHM65_00525"
     CDS             651..1880
                     /locus_tag="VHM65_00525"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX2704218.1"
                     /translation="MTDHAATRPPLPRVSRRIAAIAESATLAVDAKAKSLKAQGRPVI
                     GFGAGEPDLPTPGYIVEAAIAAASDPANHRYTPAAGLPDLRAAIATKTARDSSYVIAP
                     EQVLVTNGGKQAVYQAFATIVDPGDEVLLPAPYWTTYPEAIALAGGTSVEVFAGEDQD
                     YLVTVEQLEAARTERTKALLFCSPSNPTGAVYPPEQVRAIGEWALENGIWVITDEIYE
                     HLTYDGARAVSMPVAVPGLANTCIVVNGVAKTYAMTGWRVGWMAGPRDVIAAAANLQS
                     HLTSNVNNIAQRAAVTALAGDLTAVDEMLVSFDRRRRVMVAMLNDIEGVDCPTPRGAF
                     YVYPSVRGLLGKSIRGHVVTTSAELAALILDEAEVAVVPGEAFGPSGYLRLSYALGDA
                     DLAEGVGRIQRLLGEAR"
     gene            1922..2383
                     /locus_tag="VHM65_00530"
     CDS             1922..2383
                     /locus_tag="VHM65_00530"
                     /inference="COORDINATES: protein motif:HMM:NF039203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5709 domain-containing protein"
                     /protein_id="HEX2704219.1"
                     /translation="MSIDVDPESFTDDLGSYSIDVETQLQPGDTLVDRGVDDVLDEGY
                     SPADTPRGALAWGTTVYEQAHRETIDQRLCQEEPDVSADGDYDERQENDGRGERRAGR
                     LVSPDEGAHNHVEHTPFAADLGIDGAGASAEEAAMHVVDWDDLSRSAEDDI"
     gene            2440..>2676
                     /locus_tag="VHM65_00535"
     CDS             2440..>2676
                     /locus_tag="VHM65_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704220.1"
                     /translation="MHTLPKAHLHLHFTGSMRLGTLTGLAGHHGIRLPDSFTTSWPPR
                     LHATDERGWFRFQRLYDAARACVRGEADLRRIVHE"
BASE COUNT          417 a          967 c          920 g          372 t
ORIGIN      
        1 ggcgagtgag ccgccggtcg ggcggcgcag gctcagatgg ggcggctagt tcttggcaaa
       61 ggcgagcagg accagcttct ggaacagcag gtcgagcccg gccacgatgg ccatgatgac
      121 gagcacgaac aagatgacga cggttgtgta gttgaccagc tcggagcgag tcggccgcac
      181 caccttgcgc agctcgtcga tgatctggct gacgaacagc gacatggacc gcaagaaccg
      241 ggacaccggg ttgccggact gcccgcgctt gcgggagacc cgaccggagt cgcgggtcgc
      301 cgcgctcgtt tcggccacga ttcctcattc ctgtcgccga cggtcgtgcc gccggactgg
      361 ttcctcgacc gggcgccgac ctctgtcggc cgggcccggg tcacgcaggg caggagggac
      421 tcgaaccccc aaccgccggt tttggagacc ggtgctctac cagttgagcc actgcccttc
      481 gcggccctcg gggaggccgc accgggtggc gacgtcaggg catgcgtcag catggcagcc
      541 gtcaaccgcc gaacggacag catacgggac cgacccggcc caggtcgaac cgctcaggac
      601 gaccgcgata tccgcgcgcc ggtgccaacg gtgtctgaaa cgatgtgccc gtgaccgacc
      661 acgccgccac ccgaccgccc ctgccccgcg tctcgcgccg gatcgcggcc atcgccgagt
      721 ccgccaccct cgccgtcgac gccaaggcaa agtcgctcaa ggcccagggc cggcccgtca
      781 tcgggttcgg cgccggggag cccgacctcc cgacccctgg ctacatcgtc gaggccgcga
      841 ttgccgcggc gtccgacccg gccaaccacc gctacacgcc ggccgcgggc ctgcccgacc
      901 tgcgggcggc catcgcgacg aagactgcgc gtgactcgtc atacgtgatc gcgcctgagc
      961 aggtcctcgt caccaacggc ggcaagcagg cggtctacca ggcgttcgcg acgatcgtcg
     1021 acccgggcga cgaggtgctg ctgcccgcgc cgtactggac cacctacccc gaggcgatcg
     1081 cgctcgccgg tggcacctcg gtcgaggtct tcgcgggcga ggaccaggac tacctcgtca
     1141 ccgtggagca gctcgaggcg gcccgcaccg agcggaccaa ggcgctgctg ttctgctcgc
     1201 cgtcgaaccc caccggtgcg gtctatcccc ccgagcaggt ccgggcgatc ggtgagtggg
     1261 ccctggagaa cggcatctgg gtcatcaccg acgagatcta cgagcacctg acgtatgacg
     1321 gggcacgcgc ggtctcgatg cccgtcgcgg tgcccggcct ggccaacacg tgcatcgtgg
     1381 tcaacggggt ggccaagacg tatgcgatga ccggctggcg ggtcggctgg atggccggac
     1441 cccgcgacgt catcgcggcc gcggctaacc tgcagtcgca cctgacgtcc aacgtcaaca
     1501 acatcgcgca gcgggcagcc gtcacggcgc tggccggaga cctcacggcc gtcgacgaga
     1561 tgttggtctc cttcgaccgg aggcgacggg tcatggtcgc catgctcaac gacatcgagg
     1621 gcgtcgactg cccgacgccg cgcggcgcgt tctatgtcta cccctcggtg cgcgggctcc
     1681 tcggcaagag catccggggc cacgtggtga cgacctcggc cgagctggcg gcgctcatcc
     1741 tcgacgaggc cgaggtcgcg gtcgtgcccg gcgaggcgtt cgggccgagc ggctacctgc
     1801 gtctctccta cgccctcggc gacgcggatc tggccgaggg tgtgggccgg atccagcgcc
     1861 tgctcggcga ggcgaggtag gtcacctccc acgcagcccc catgcgtggc agagtggatt
     1921 catgagcatc gacgtcgacc ccgagagctt caccgacgac cttgggtcgt actcgatcga
     1981 cgtcgagacc cagctgcagc ccggcgacac gctggtcgac cgtggcgttg acgacgtgct
     2041 cgacgagggg tactccccgg ccgacacccc ccgcggggcg ctggcctggg ggacgacggt
     2101 ctacgaacag gcccaccgcg agacgatcga ccagcgcctg tgccaggagg agcccgacgt
     2161 cagtgccgac ggcgactacg acgagcggca ggagaacgac ggtcgcggcg agcgccgggc
     2221 cggacgcctc gtctcccccg acgagggcgc ccacaaccac gtcgagcaca caccgtttgc
     2281 cgccgacctc gggatcgacg gcgccggagc atcggccgag gaggccgcga tgcacgtcgt
     2341 cgactgggac gacctgtctc gctcggccga ggacgacatc tgacccgctc gttgctggcc
     2401 ccgccgtccg ggagcctggc ccggcccggg ccgaggtccg tgcacacctt gcccaaggcc
     2461 catcttcacc tgcacttcac ggggtcgatg cgcctgggca ccctgaccgg tctcgcgggc
     2521 caccacggga tccggctgcc tgactcgttc acgacgtcgt ggccgccgcg actgcatgcc
     2581 acggacgaac gcggctggtt ccgtttccag cgcctgtatg acgccgcccg tgcctgcgtg
     2641 cgaggcgagg ccgacctgcg ccggatcgtg cacgag
//
LOCUS       DATAIX010000032         3027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_91255, whole genome shotgun sequence.
ACCESSION   DATAIX010000032 DATAIX010000000
VERSION     DATAIX010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91255"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..320)
                     /locus_tag="VHM65_00540"
                     /pseudo
     CDS             complement(135..320)
                     /locus_tag="VHM65_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
     gene            complement(441..1064)
                     /locus_tag="VHM65_00545"
     CDS             complement(441..1064)
                     /locus_tag="VHM65_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704221.1"
                     /translation="MALAAGEVWTWGDGSKGALGLGNLTDRSTPTRVPGLSGVTDVTT
                     GHYHSLALLADRTVRTWGQNLYGQLGDGSTTRRTSPVTVAGLSDVVEVAGGADMSYAR
                     RADGTVWAWGRGDRGELGNGTLTRKLVPNTVTGLPAIAQVGCGRDHTLAISATGTLYA
                     WGVNDFGQLGDGTLTRRTRPVLVPGASNVLQAHGGRGYSVVRRGPLP"
     gene            complement(1176..1301)
                     /locus_tag="VHM65_00550"
     CDS             complement(1176..1301)
                     /locus_tag="VHM65_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704222.1"
                     /translation="MTSKPRRLRRLLPLLAALTTLAVAFVPSPRGGSGGDRVLTT"
     gene            complement(1385..2308)
                     /locus_tag="VHM65_00555"
     CDS             complement(1385..2308)
                     /locus_tag="VHM65_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEX2704223.1"
                     /translation="MRGWQPSGEQWRLRHGDDEAVVVEVGGGLRTYRAAGVDVLAGYR
                     SDQMCPQGRGQALMPWPNRIRDGRYTFAGTCYQLPVTEVGRANASHGLVRWAEWDRID
                     HTSDTVTVITVVHPQPGWAWRLELATTYRLTHDGLSVLTQVRNASPSPAPFGYGAHPY
                     LALAGVAAPDTVLTVPAARYLEVDPERRLPVATHPVDGTEADLRHPHALGDTELDTAF
                     TGVGRDADGLWRVRLDRGAAGVVTLWADEAFGWLQVFTAKARVGAAHERGIAVEPMTC
                     PPDAFNSGDSLVVLEPGASWSGRWGIVPPAA"
     gene            2448..>3027
                     /locus_tag="VHM65_00560"
     CDS             2448..>3027
                     /locus_tag="VHM65_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704224.1"
                     /translation="MPTSARSLADDIRARSDVQLSGLVQARPDLARPAPSDLTTLAAR
                     ASTRASVQQALAGLDRGLLDVLEGALAASDSTGCRPVSAREIAQAVGVRTVGEIRDHI
                     DTLWLRAVLWRAADGLRAPRAVIELLGPTPNGLGPSAAELSGLAPAVTAAKARALRAL
                     LARAPDAASAVLDSLAWGPPIGVLGASPAAGEG"
BASE COUNT          404 a         1116 c         1101 g          406 t
ORIGIN      
        1 ccccggcgca gcgagttcga gacgttgtcg aggagcttga tgacatcctc gacgatgacg
       61 accatgtcgt cggcgggctt gcgatgacgc tcccgctggg cggcggcaac cgatggcacg
      121 gggtcgagga cgcgcaagga cagcgcctgg gccccccgac gcccctcgac gatgccgaac
      181 tcgacgcggg tgccgccctt gagggtcttc acaccctccg gcagcgcatt ggcgtgcagg
      241 aagacgtcgc cgccgtcgtc gtcggagagg aagccgaagc ccttctcccc gtcataccac
      301 ttgaccttgc cagtcggcac tgccgttacc tcacgttcgt caggatgacc ccatgagagg
      361 gcgccaccgc gcctgtcgcg cacacacaga ctatcggtcc gctgggggtg gctcccgctg
      421 gtttaccggt ctccatctgg ttacggcagg gggccccggc gaaccacgga gtagccccgt
      481 cccccatggg cctgcagcac gttcgaggca ccgggaacca ggaccggccg ggtccggcgg
      541 gtcagcgtcc cgtcgccgag ctggccgaag tcgttgacac cccacgcgta gagcgtcccc
      601 gtcgccgaga tggccagggt gtggtcacgg ccacagccca cctgcgcgat ggcgggcagc
      661 ccggtgacgg tgttcgggac gagcttgcgg gtcagggtcc cgttgccgag ctcgccacgg
      721 tcgccgcggc cccaggccca gaccgtcccg tcggcgcgcc gcgcgtagct catgtccgcg
      781 ccaccggcca cctcgacgac atcgctgagg ccggccaccg tgaccggtga ggtgcgccgt
      841 gtcgtcgagc cgtcgccgag ctggccgtag aggttctggc cccaggtcct gaccgtgcgg
      901 tcagcgagca gggcgagcga gtggtagtgg cccgtcgtca cgtcggtgac gccgctcagc
      961 ccgggaacgc gggtgggggt cgagcggtcg gtgaggttgc cgaggccgag cgcgcccttg
     1021 gagccgtcgc cccaggtcca cacctcccca gccgccagcg ccacgacatg ctcacgccca
     1081 ccggcggcgt cgtcggggtc ggtgagcggg gtgatgagtc ccaggctcgt acggctcgcc
     1141 gtgttgcccg tgccgagctg gccgtccgag cccagtcagg tcgtcaggac ccggtccccc
     1201 ccgctgcccc cgcgggggga cgggacgaac gcgaccgcca gggtggtcag cgccgcgaga
     1261 agggggagta gccggcgaag acgccggggc ttcgatgtca tgggtgtacc tcaagccttg
     1321 agtatgccga gccagagccg gtccgcgggc cggttccgcg cagatcgcgc gagaacgggc
     1381 cgggtcaggc cgcgggtggc acgatccccc agcgcccgct ccaggacgcg ccgggctcga
     1441 ggacgacgag cgagtcgccc gagttgaacg cgtccggcgg gcaggtcatc ggctcgaccg
     1501 cgatgccgcg ctcgtgcgcc gcaccgaccc gcgccttggc cgtgaacacc tgcagccacc
     1561 cgaacgcctc gtctgcccac agtgtcacca cgccggccgc gccccggtcg agccggaccc
     1621 gccagaggcc atcggcgtcg cgcccgactc cggtgaaggc cgtgtcgagc tcggtgtcac
     1681 cgagcgcgtg cgggtggcgc aggtcggcct cggtcccgtc gacggggtgg gtcgcgaccg
     1741 ggagccgccg ctcggggtcg acctcgagat agcgcgccgc cgggacggtc aggacggtat
     1801 cgggcgccgc gacgccggcg agcgccaggt acgggtgagc gccatacccg aacggtgcgg
     1861 ggctgggact ggcgttgcgc acctgggtga gcacgctcag cccatcatgg gtgagccggt
     1921 atgtcgtcgc cagctcgagg cgccaggccc acccgggctg cgggtggacg acggtgatga
     1981 ccgtgaccgt gtcggaggtg tggtcgatgc gatcccactc ggcccagcgg acgagcccgt
     2041 ggctcgcgtt ggcgcgcccg acctcggtga ccgggagctg gtagcaggtc ccggcgaagg
     2101 tgtagcgccc gtcgcggatc cggttgggcc acggcatgag cgcctgcccg cgcccctggg
     2161 ggcacatctg gtcggaccgg tagcccgcga ggacgtcgac gccggcggca cggtacgtcc
     2221 gcagcccgcc gccgacctcc accacgacgg cctcgtcgtc cccgtggcgc agccgccact
     2281 gctcaccgct tggctgccaa ccccgcatgc ccaccaacag tagtgaccct ccccgctcac
     2341 tcgaacagga cgaactcgcc aatcctgacc gttttgcccc attatcaggg tattctgcgg
     2401 tggcttgacc agttcgacgg accgaccagc cgggatacgg ttgagggatg cccaccagcg
     2461 cgcgcagcct cgccgacgac atccgcgcgc gcagcgacgt ccagctgtcc gggctcgtgc
     2521 aggcccggcc cgacctcgcc cgccccgcgc cgtcggacct gacgaccttg gccgcccggg
     2581 ccagcactcg ggccagcgtc cagcaggccc tcgccgggct ggaccgcggc ctgctcgacg
     2641 tcctcgaagg cgcgctcgcc gcatcggact cgacgggctg ccgcccggtg tccgcgcgcg
     2701 agatcgccca ggccgtcggt gtccggacgg tcggggagat ccgcgaccac atcgacacgc
     2761 tctggctccg tgcggtgctg tggcgcgcgg ccgacgggct gcgggcgccg cgcgcggtca
     2821 tcgagctgct cgggccgacg cccaacggac tcgggcccag cgccgctgag ctctccgggc
     2881 tcgccccggc cgtcacggcg gcgaaggcca gggcgctccg ggcgctgctg gcccgggcgc
     2941 ccgacgcggc gtcggccgtg ctcgactcgc tggcctgggg gcccccgatc ggggtgctcg
     3001 gcgccagtcc cgcggccggc gaaggag
//
LOCUS       DATAIX010000033         1676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_119995, whole genome shotgun sequence.
ACCESSION   DATAIX010000033 DATAIX010000000
VERSION     DATAIX010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119995"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VHM65_00565"
     CDS             complement(<1..368)
                     /locus_tag="VHM65_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX2704225.1"
                     /translation="MRHTEVVLAEIREQPLSVDEVLAAVRHPEAGAVSLFVGMVRDHD
                     HGADVAGLDYSAHPSATETARTMAERIAGRGTAVRIAVVHRVGSLAVGDLAIVAAVSA
                     AHRSEAFSACRELVDGFKTTV"
     gene            complement(368..1471)
                     /locus_tag="VHM65_00570"
     CDS             complement(368..1471)
                     /locus_tag="VHM65_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2704226.1"
                     /translation="MSSPTTANPAGGRGRTVAVAGAASSVPGQIISALTTRALRRGGP
                     ISTVIGVDTVPGGAGSVPWRLADLRSPALASVLAGVDTVVWVACSTDLQHDLERRASE
                     RRAEVTRTALTVSMAAAAAGVSHLVVVSSAMVYGAAPDNPVPLTEDSARRAVSDEGLV
                     GDLLAVEDVLERSRLDHPGLAVTLVRPAALVGAGVDTVVTRHFAAPRLLAVKGTTPAW
                     QFCHVEDLASGVAETVALGLSPVVTVGSLGSMTQAEVERLSGMRRVELSESAALGTAA
                     RLHRIGVLPSPASDLSYALYPWVVSSETLRATAWEPTYDNATALGVLLDGVRGQYAFA
                     GRRVERKDAALGAASAAVALVGTAAILRRARRK"
BASE COUNT          258 a          602 c          614 g          202 t
ORIGIN      
        1 actgtcgtct tgaagccgtc gacgagctcg cgacaggccg agaacgcctc ggaccgatga
       61 gcggcgctga ccgcggcgac gatcgcgagg tcgccgacgg cgagcgagcc gacccggtgg
      121 accaccgcga tccgcacggc cgtgccccgg cccgcgatcc gctcggccat ggtccgggcc
      181 gtctcggtcg cactcgggtg cgcggagtag tccaggccgg cgacgtccgc gccatggtcg
      241 tggtcacgga ccatgccgac gaacaacgac accgctccgg cctcggggtg ccggacggca
      301 gcgagcacct cgtcgaccga gagcggctgc tcgcggatct cggcgaggac gacctcggta
      361 tgacgcatca cttcctcctc gcccgtcgca ggatggcagc ggtcccgacc agcgccacgg
      421 cagcgctggc tgctccgagg gcggcgtcct tgcgctcgac ccgccgcccc gcgaaggcgt
      481 actggccgcg gaccccgtcg agcagcaccc cgagcgccgt cgcattgtca taggtcggtt
      541 cccacgcggt ggcgcgcagg gtctcactgg acacgaccca ggggtagagc gcataggaca
      601 ggtcgctggc cggggacggg agcacaccga tccggtgcag ccgggccgcg gtccccagtg
      661 cggcactctc gctcagctcg acccggcgca tacccgagag ccgctcgacc tccgcctggg
      721 tcatcgagcc cagggacccg acggtgacga ccgggctcag gccgagggcc acggtctcgg
      781 cgacaccgga agccaggtcc tcgacgtggc agaactgcca ggccggggtc gtccccttga
      841 cggcgagcag gcggggggcc gcgaagtgcc gagtcaccac ggtgtccacc cccgcgccga
      901 cgagagcggc cggacgcacg agcgtgacgg ccagccctgg atggtcgagg cgggaccgct
      961 cgaggacatc ctcgaccgcg agcaggtctc cgacgagccc ctcgtcggaa acggcccgcc
     1021 gagccgagtc ctcggtgagc ggcaccgggt tgtcgggggc ggcgccatac accatggcac
     1081 tggacacgac gacgaggtgc gagaccccgg cagcggccgc ggccatcgag acggtcaggg
     1141 cggtccgggt cacctcggcc cgccgctcgc tcgcgcggcg ctcgaggtcg tgctgcaggt
     1201 cggtgctgca ggccacccag acgacggtgt cgaccccggc caggacgctc gcgagcgccg
     1261 gggagcgtaa gtccgcgagg cgccacggca cgctgccggc ccctccagga acagtgtcga
     1321 cgccgatcac cgtgctgatc gggcccccac ggcgaagggc acgggtggtc agcgccgaga
     1381 tgatctgccc cgggacggat gaggccgcgc ccgcgacggc gacggtgcgg ccacggccgc
     1441 ccgcagggtt cgcggtcgtc ggggaactca catcggcctc agggggttgt ctagggggtg
     1501 ggtgccaggg acatcatgtc cgtaggcacg gcgtccgtgc cattgtcacc cgtgacgaaa
     1561 ccagcccggt gtcatcacga agtctcgtcc acagtcgcag gaggcgtgga agccaggtca
     1621 gacactcccc aggaccgggg cggcgagggt ccgcaggacc tgtccgcgat gctgtc
//
LOCUS       DATAIX010000034         5238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_91347, whole genome shotgun sequence.
ACCESSION   DATAIX010000034 DATAIX010000000
VERSION     DATAIX010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91347"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VHM65_00575"
     CDS             <1..450
                     /locus_tag="VHM65_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704227.1"
                     /translation="ALAASVGGLGVTAVPDPAAGLNAAVTAGLDSAAARWPEAARAVL
                     LADVPALRPGDLASALGAAAAYPRSFVPDHTGTGTVLLTARAGEPLVPHFGADSARRH
                     TAAGAVRLDLDLPGLRTDVDDAASLALVLALGAGPRVAALAREAGLL"
     gene            461..676
                     /locus_tag="VHM65_00580"
     CDS             461..676
                     /locus_tag="VHM65_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704228.1"
                     /translation="MQATVHAFDDTTGSGVLIGDDGLLFDLPTVAFAGSGLRHLRPGQ
                     RLTIERTEDGAVTRVWMVGVGDGQPVY"
     gene            complement(875..1129)
                     /locus_tag="VHM65_00585"
                     /pseudo
     CDS             complement(875..1129)
                     /locus_tag="VHM65_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
     gene            complement(1336..1929)
                     /gene="leuD"
                     /locus_tag="VHM65_00590"
     CDS             complement(1336..1929)
                     /gene="leuD"
                     /locus_tag="VHM65_00590"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX2704229.1"
                     /translation="MDRFTTHTGIGVPLRRSNVDTDQIIPAEYLKRVARDGFEDGLFA
                     AWRKDASFVLNQGPYAAGSVLVAGPEFGTGSSREHAVWALMNYGFRVVLSSRFADIFR
                     GNSGKQGLLTGQVAQGDIELIWKYLENTPGASVTVDLVSRTVTAGELVAVFDIDDYTR
                     WRLLEGLDDIGLTMRHGEEVADFETRRPPYRPVTHSS"
     gene            complement(1933..3339)
                     /gene="leuC"
                     /locus_tag="VHM65_00595"
     CDS             complement(1933..3339)
                     /gene="leuC"
                     /locus_tag="VHM65_00595"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF004016.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX2704230.1"
                     /translation="MATTLAEKIWESHVVRRADGEPDLLYIDLHLVHEVTSPQAFDGL
                     RLAGRTVRRPELTLATEDHNVPTTPGPIVDPVSRTQVETLRRNCAEFGVRLYPMGDAE
                     QGIVHVVGPQLGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQALPLRP
                     FKTMAVTVEGTLAPGVTAKDIILTVIATIGTGGGQGYVLEFRGSTIRALSMEARMTVC
                     NMSIEAGARAGLIAPDDTTLAYLQGRSHAPTGVAWDAAVADWRGLKTDADALFDTEIH
                     IDAGTLTPFVTWGTNPGQGLPLSASVPDPSAIVDEGERAAAERALEYMGLAPGVPLRD
                     IAVDTVFVGSCTNGRIEDLRAAAEVVKGRRVAKGVRMLVVPGSAKVRLQAQAEGLDQV
                     FTDAGAEWRLAGCSMCLGMNPDQLAPGERSASTSNRNFEGRQGRGGRTHLVSPLVAAA
                     TAIRGTLCAPADLDTVRA"
     gene            3436..4167
                     /locus_tag="VHM65_00600"
     CDS             3436..4167
                     /locus_tag="VHM65_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX2704231.1"
                     /translation="MDNSSGVGVLDKAAAVLSALEAGPATLAQLVTATGLARPTAHRL
                     AVALEHHRLVSRDMQGRFVLGPRLAELSSAAGEDRLLAAAGPVLGALRDHTNESSQLF
                     RRQGDQRICVAAAERPMGLRDTIPVGATLSMLAGSAAQVLLAWEEPDRLHRGLRGAKF
                     NATALSAVRRRGWAQSVGEREPGVASVSAPVRSPSGRVVAAVSISGPIERLSRQPGRL
                     HAGTVVAGANKLTEVLRRAHATHHG"
     gene            4277..5155
                     /locus_tag="VHM65_00605"
     CDS             4277..5155
                     /locus_tag="VHM65_00605"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX2704232.1"
                     /translation="MPPTLARCGVLAALLAAAMMLSAGCAPQAATAPAATQSWTSSPA
                     TAAPSLESPSPTPSETTTTTPTPTTTPKAPPKPVAGPAILKKGSTGDKVRELQARLKQ
                     IGWWSGGIFPTYGPRTTAAVKGFQGKRRFPVTGEVDQRTWDRIVSMTHKPTKAELTYV
                     VPEPIPGVKGLDPRCMTGRAICASKTTRQVVWVVDGRVVLRFSARFGRSSLPTSNGVF
                     HVYWKSRDHWSTKYDAPMPFALFFNGGQAVHYSPEFASIGYGGVGSHGCVNLRDRSGA
                     ESLFDLVRVSDAVVVY"
BASE COUNT          694 a         1884 c         1848 g          812 t
ORIGIN      
        1 gcgctggccg cctcggtcgg tgggctcggc gtcaccgccg tcccggaccc ggcagccggt
       61 cttaatgcgg ccgtcaccgc tgggctcgac tcggcggcgg cccgctggcc cgaggccgcc
      121 cgggccgtcc tgctcgccga cgtgccggcc ttgcgacccg gcgacctcgc ctcggcactc
      181 ggcgccgccg cggcctaccc tcgctcgttc gttcctgacc acaccggcac cggcacggtc
      241 ctgcttacgg cccgagccgg tgagccgctg gttccgcact tcggcgcgga ctcggctcgc
      301 cggcataccg ccgccggcgc cgtgcgactc gaccttgacc tcccggggtt gcgcaccgac
      361 gtcgacgacg ccgcctccct ggcactcgtg ctggcactcg gggcgggccc ccgggtcgcg
      421 gccttggctc gcgaggccgg cctgctctaa cgtctagctc atgcaggcaa ccgtgcacgc
      481 gttcgacgac accactggct caggcgtcct catcggcgac gacgggctgc tcttcgacct
      541 cccgaccgtc gcgttcgcgg gttcggggct gcgccacctg cgccctggcc agcgcctgac
      601 catcgagcgc accgaggacg gcgccgtgac ccgcgtctgg atggtgggtg tcggcgacgg
      661 ccagcccgtc tactgatgct cccgacggtg tatggcgcag cgactacttc gtgctcgctc
      721 gcttggcggg ggccttcttc gccgccgcct tcgtgggagc tttggtggga gcgttggcct
      781 gggtcgccga cgcgcggcgg atcaccgaca cggtagcggc atgggccccg tcgggcgtcg
      841 ccttcgccag tttgcggggg tcggcgacat aggtcttgaa cgccgttccg gccttgaact
      901 tcggcacggt cgtcttcttg atccggacgg tctgcccagt gcgggggttc cgacccgtcc
      961 gggcggcgcg cgcggcgccc tcgaacgtcc cgaagccggt gatcccgacc ttccccccct
     1021 tggcgacctc acggatgatg gcatcgaaga tggcctcgag ggcatcggag gcggccttct
     1081 tgctcccgag gcgcgactcc accgccgcaa tgagctcagc cttgttcacg ttttcccctt
     1141 tgtcggacct cttgagccgc agacctcgtg tctgacggcg gcgcggccag agcctatcgg
     1201 cttgggtggg cccgtgaggg gccggaggtc ccggagtcca tcacttccgt ggcgttgctc
     1261 gccttaggcc gcggtgagca gctggcggct cccctcgcgg ggaccccgtt ccgcggctcg
     1321 gggttggtga tggcttcagc tggagtgcgt caccgggcgg tagggcggac gccgtgtctc
     1381 gaagtctgcg acctcctcgc catggcgcat ggtcagcccg atgtcgtcga gcccctcgag
     1441 caggcgccag cgcgtgtagt cgtcgatgtc gaagaccgcg acgagctccc cggcggtgac
     1501 ggtgcggctg accaggtcga cggtgaccga ggcgcccggg gtgttctcga ggtacttcca
     1561 gatcagctcg atatcgccct gcgcgacctg cccggtgagc agcccctgct tgcccgagtt
     1621 cccgcggaag atgtcggcga accgcgagga gagcacgacc cggaagccgt agttcatgag
     1681 agcccagacg gcgtgctcac gagaggagcc ggtgccgaac tccgggcccg cgacgaggac
     1741 cgagcccgcg gcatacgggc cctggttgag cacaaagctc gcgtccttgc gccaggccgc
     1801 gaacagcccg tcctcgaagc cgtcgcgggc gacccgcttg aggtactccg cggggatgat
     1861 ctggtcggtg tcgacgttgc tgcggcgcag cgggacgccg atgccggtgt gtgtcgtgaa
     1921 gcggtccacg gcctaggccc gcacggtgtc aaggtcggca ggtgcgcaca gagtcccgcg
     1981 gatggcggtc gccgcggcga ccagcgggct cacgagatga gtgcgcccgc ccctgccctg
     2041 gcgtccctcg aagttgcggt tggatgtcga cgcactgcgc tcgccggggg cgagctggtc
     2101 cgggttcatg cccaggcaca tcgagcaccc ggccaggcgc cactcggccc cggcgtcggt
     2161 gaacacctgg tccaggccct cggcctgggc ctggaggcgc accttggcgg acccgggaac
     2221 gacgagcatc cgcacgccct tggccacgcg ccgacccttg acgacctcgg ccgccgcgcg
     2281 caggtcctcg atccgcccgt tggtgcacga gccgacgaag accgtgtcaa ctgcgatgtc
     2341 ccgcaacgga actcccgggg cgaggcccat gtactcgaga gctcgctcgg ccgcggcgcg
     2401 ctcgccctcg tcgacgatcg ccgacgggtc cgggacgctc gcgctgagcg gaaggccctg
     2461 accggggttg gtcccccagg tgacgaacgg ggtgagcgtg ccggcgtcga tgtggatctc
     2521 ggtgtcgaag agtgcgtcgg cgtcggtctt cagcccgcgc cagtcggcga ccgcggcgtc
     2581 ccaggccacg ccggtcggcg cgtgcgaccg gccctggagg taggccaggg tcgtgtcgtc
     2641 gggggcgatg agcccggccc gggcaccggc ctcgatcgac atgttgcaca cggtcatccg
     2701 ggcctccatc gacaaggccc tgatggtgct gccgcggaac tccaggacgt agccctgacc
     2761 gccccccgtc ccgatcgtgg cgatgacggt gaggatgatg tccttggccg tgactcccgg
     2821 cgccagggtg ccctcgaccg tgacggccat cgtcttgaag ggccgcagcg gcagggcctg
     2881 cgtcgcgagg acgtgctcga cctcgctggt cccgatcccg aaggcgaggg cgccgaaggc
     2941 tccgtgggtc gaggtgtggc tgtcgccgca gacgatggtc atcccgggct gggtgaggcc
     3001 gagctggggg ccgacgacat ggacgatgcc ctgctcggcg tcgcccatcg ggtagagccg
     3061 gacgccgaac tcggcgcagt tgcgccgcag ggtctcgacc tgggtcctgc tcaccgggtc
     3121 cacgatcggc cccggcgtcg tcgggacgtt atggtcctcg gtcgccaggg tgagctcggg
     3181 ccgacggacc gtccgtccgg cgaggcgcag accgtcgaac gcctgggggc tggtcacctc
     3241 gtggacgagg tgcaggtcga tgtagagcag gtccggctcg ccgtcggcac gacggacgac
     3301 gtgcgactcc cagatcttct cggccagcgt cgttgccatg gggccacctc cgtgatactc
     3361 cagtaccaaa caggttcgcg cacttgtcgg gctcccgaag ttgcgtctca gtgagtgaga
     3421 tggcagtatc agagcatgga caacagcagt ggggtgggcg tcctggacaa ggccgccgcg
     3481 gttctgagcg ccctggaggc cggaccagcc accctcgcgc agctcgtcac ggcaacgggg
     3541 ctggcccgcc cgacggctca ccggctcgcg gtggcgctcg agcaccaccg cctcgtgagc
     3601 cgcgacatgc agggccggtt cgtcctcggc ccgcgcttgg ccgagctgtc ctccgccgcc
     3661 ggcgaggatc gcctcctcgc ggccgccggc ccggtcctcg gtgcgctgcg tgaccacacg
     3721 aacgagagtt cccagctgtt ccgccgccag ggggaccagc ggatctgtgt cgctgctgcc
     3781 gagcgcccca tgggcctgcg cgacaccatc ccggtcggcg ccaccctgtc gatgctggcc
     3841 ggatccgccg cccaggtgct cctggcctgg gaggagcccg accggctgca ccgagggctg
     3901 cgtggggcga agttcaacgc caccgccctg tcggccgtcc gccgtcgcgg ctgggcgcag
     3961 agcgtcggcg aacgcgagcc gggagtggcc tcggtgtcag ccccggtgcg cagcccgtcc
     4021 gggcgggtcg tggccgcggt gtcgatctcg ggcccgatcg agcggctgtc gcgccagcct
     4081 gggcgcctcc acgccggcac cgtcgtcgcc ggagcgaaca agctcaccga agtgttgcgc
     4141 cgggctcacg ccactcatca cgggtagatt cgaggtgcca ctgtgacgca tgaggcgttg
     4201 gtggcaacct cggacgcggt ctgcgcgtct ctaagtggag cggggggccc ctcgctcacc
     4261 gcaggaagga gccccggtgc cgccgaccct cgcccgctgc ggcgtgctcg ccgcgctgct
     4321 cgctgccgcc atgatgctca gtgccgggtg cgcgccccag gccgcgaccg ccccagcggc
     4381 gacccagtcc tggacgtcca gccccgccac tgcggcaccc tcgctggaga gcccgtcgcc
     4441 gacgccgagc gagaccacga cgaccactcc gactccgacg acgaccccga aggctccccc
     4501 taagccggtc gccgggccgg ccatcctcaa gaaggggtcg accggtgaca aggtccgtga
     4561 gctgcaggcc cggctcaagc agatcggctg gtggtccggc ggcatcttcc cgacctacgg
     4621 accacgaacc acggcagcgg tcaagggctt ccagggcaaa cgccgcttcc ccgtcaccgg
     4681 cgaggtggac cagcgcacgt gggaccggat cgtgtcgatg acccacaagc cgacgaaggc
     4741 cgagctgacc tacgtcgtgc ccgagcccat cccgggcgtc aaaggcctcg accctcgctg
     4801 tatgacgggc cgcgccatct gtgccagcaa gacgacgcgc caggtcgtgt gggtcgtcga
     4861 cggcagggta gtgcttcgct tcagcgcccg gttcggccgc tcgtcgctgc cgacgtcgaa
     4921 cggggtcttc cacgtgtact ggaagtcgcg cgaccactgg tccaccaagt acgacgcgcc
     4981 catgcccttc gcgctgttct tcaacggggg ccaagcggtg cactactccc ccgagttcgc
     5041 ctcgatcggc tacggggggg tcggctcgca cggatgcgtc aacctgcggg accgcagcgg
     5101 cgccgagtcc ctcttcgacc tagtccgcgt gagcgacgcc gtcgtcgtct actagctggg
     5161 gactcaaccc cgcgcgaggc gagctccacg acataccggc tgggtgcacg ccgtaggctc
     5221 ggcccatgct gaagatcg
//
LOCUS       DATAIX010000035         3477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_31872, whole genome shotgun sequence.
ACCESSION   DATAIX010000035 DATAIX010000000
VERSION     DATAIX010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31872"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(432..1127)
                     /locus_tag="VHM65_00610"
     CDS             complement(432..1127)
                     /locus_tag="VHM65_00610"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX2704233.1"
                     /translation="MTDLIDTTEMYLRTVFELEEEGILALRARIAERLGHSGPTVSQT
                     VARMERDGLLTLSGDRHLELSESGRLLATRVMRKHRLAERLLVDVIGLEWEYAHDEAC
                     RWEHVVSERVERRIVSMLGEVHESPYGNPIPGLEEFGIEPSVGFRAGVVALAEVAGPV
                     PLVVVVRRIGEPAQVDPESLAVLARAGIAPGRSIRVQAAGQRVLAVDAAAGDGEAISL
                     PREVSAHVFVSTA"
     gene            1148..2590
                     /locus_tag="VHM65_00615"
     CDS             1148..2590
                     /locus_tag="VHM65_00615"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX2704234.1"
                     /translation="MTPTVLWLRRDLRLLDHPALGAAHAAGGGGPVLPLFVIDPALWA
                     GSSPARRCRLAASLRSVHARYDGRLVVRTGSPELVVAQVAAEAGATSVHISAETTPYG
                     RRRDGRVRQALAAQGRPLVATGTPYAIGPGALLTAGGTPFQVFTPFRRAWLARGWPQP
                     APVPPGLRLARGVESEPLPRVAEDAPGGAHEAPGELVALDRWAAFAQDGLDRYSERRN
                     RPDLDGTSRLSEALKYGEVHPRTLLAGMAGLHGSGSAGAADFTSELCWREFYADVLWH
                     QPHSAWSDLKPALARMTYDEPGEAAEAWAAGRTGYPIVDAGMRQLLTEGWMHNRVRML
                     TASFLVKDLHVWWPYGARVFLRHLRDGDLASNSHGWQWTAGTGTDAAPYVRIFNPVTQ
                     GLRFDPDGDYVRRHVPELGHLRGAGAHEPWAVPDGYAQGYPQRIVDHASERTEALRRY
                     AEARQSARQGDLALDRAAGVTAGARARTRS"
     gene            complement(2556..3134)
                     /locus_tag="VHM65_00620"
     CDS             complement(2556..3134)
                     /locus_tag="VHM65_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX2704235.1"
                     /translation="MARAQAGRRPVTRERLFERALAIIDAGGLEALTMRRLAADVGVE
                     AASLYHHVSGKDELLDGALSLMRSEMVFAEPLPQDWRALMELVFTTYLAVLAAHPNML
                     PLAGRRVGTDPQSGLVYLVSSGFPEEDAVELWQSINAFTVGFAAFSSAFTTTDTSDLP
                     EGLARRMAQWRETTSRRTLGLIMTGYEPSRRR"
     gene            3236..>3477
                     /locus_tag="VHM65_00625"
     CDS             3236..>3477
                     /locus_tag="VHM65_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704236.1"
                     /translation="MSSAQTSAPTQAPTTSQAPSTAARRTDRWRTLYLIGATAALVGV
                     GGALLDIVLTSLPGWRPDTVPRTLAGWFGQLDDRPWL"
BASE COUNT          492 a         1314 c         1211 g          460 t
ORIGIN      
        1 gacgatgggt gttcaggcag ctccccgggg cctactgcca cgcctgacag cacccggacg
       61 gcaacgcgct cactcgccgc tgcgggtgag acaagggcgg caccattcgc ggacgccgta
      121 tcgacggagc gcgccaccac gtgccgcccg aacgccgcca aaaagcgccg caacctaccc
      181 tcccgatcat gccgtgccgt cctaccctcg tcggtgtcgg ggcggggggc atcgccggtg
      241 gcacgatcgc agacgttctt cctgggcgag ccacgccagc catgactcga ctctggccag
      301 cgaatgcagc gcccgcacca agtcgtcgac gacacaccgg tccgcggtgg gcagggcagc
      361 cagcaccgcc gtgatcgacc tcgccaccac cagccgccac gagtgaggtt gacctggctg
      421 gggccgcccc cctacgccgt ggagacgaac acgtgggcgc tgacctcccg gggcagcgag
      481 atggcctcgc catccccggc ggcggcgtcg acggcgagga cccgctgccc ggcagcctgt
      541 acccggatcg accggccggg ggcgatgccc gcgcgcgcca ggacggcgag cgactccggg
      601 tcgacctggg caggctcgcc gatccgccgg acgaccacga cgagcggcac cggcccggca
      661 acctcggcca gcgccaccac acctgcccgg aaccccacag agggctcgat accgaactcc
      721 tcgagccccg ggatcgggtt gccgtagggc gactcgtgga cctcgccgag catgctcacg
      781 atccggcgct cgacccgctc gctcacgacg tgctcccagc ggcaggcctc gtcatgggcg
      841 tactcccact ccagcccgat gacatcgacg agcagccgct cggcgaggcg gtgcttgcgc
      901 atcacccggg tcgcgaggag acgccccgac tcggacagct cgaggtgacg gtcgccggac
      961 agcgtgagca gcccgtcgcg ctccatccgg gccacggtct gcgacacggt gggcccggag
     1021 tggcccagcc gctcggcgat gcgcgcgcgc agggccagga tgccctcctc ctcgagctcg
     1081 aagacggtcc gcaggtacat ctcggtcgtg tcgatcagat ccgtcacgtg caatagcctc
     1141 tcactcgatg actccaacgg tgctctggct gcgccgcgac cttcgcctgc tcgaccaccc
     1201 tgccctcggc gcagcgcacg cggccggcgg cggcggcccg gtcctgccgc tcttcgtcat
     1261 cgaccccgca ctctgggcag gctcctcccc cgcgcgccgc tgcaggctcg cggcctcgtt
     1321 gcgctcagtg cacgcacggt atgacgggcg cctcgtcgtg cgcaccggct cacccgagct
     1381 cgtcgtcgcc caggttgccg ccgaggcggg agccactagc gtccacatca gtgccgagac
     1441 gacgccgtac ggccggcgcc gcgacgggcg ggtccgccag gccctggcgg cccagggccg
     1501 cccgctcgtg gcgaccggca cgccatacgc catcggacct ggggccctgc tcacggcagg
     1561 ggggacgccg ttccaggtgt tcacaccgtt ccgccgggcg tggctcgcac gtggctggcc
     1621 gcagcccgcg ccggtgccgc cggggcttcg cttggcgcgg ggcgtcgaga gcgagccgct
     1681 gccccgggtc gccgaggacg cgcccggcgg tgcgcacgag gcgcccggtg agctcgtggc
     1741 gctcgaccgg tgggccgcct tcgcgcagga cgggctcgac cgctactccg aacggcgcaa
     1801 ccgtccggac ctcgacggga cctcacggct gtccgaggcg ctcaagtacg gtgaggtgca
     1861 tccgcggacg ctgctcgccg ggatggccgg tctccacggg agcggttcgg cgggcgccgc
     1921 ggacttcacg agcgagctgt gctggcgcga gttctacgcc gacgtcctgt ggcaccagcc
     1981 gcactcggcc tggtcggacc tcaagcccgc gctggcccgg atgacgtatg acgagccggg
     2041 cgaggcggcc gaggcgtggg cggcggggcg cacgggctac ccgatcgtcg atgccgggat
     2101 gcgccagctg ctcaccgagg gctggatgca caaccgggtc cggatgctca ccgcgagctt
     2161 cctcgtcaag gacctgcacg tgtggtggcc gtatggcgcc cgcgtgttcc ttcgtcatct
     2221 gcgcgacggc gacctcgcct cgaactccca cggctggcag tggaccgccg gcacgggcac
     2281 cgacgcggcg ccatacgtcc ggatcttcaa cccggtcacc caggggctgc gtttcgaccc
     2341 ggacggcgac tatgtccggc gccacgtccc cgagctgggc cacctccgcg gtgctggcgc
     2401 gcacgagccg tgggcggtgc cggacggcta tgcccagggc tacccgcagc gcatcgtcga
     2461 ccacgccagc gagcggaccg aggccctgcg ccggtatgcc gaggcccgcc agtcagcccg
     2521 gcagggcgac ctcgcgctgg accgtgctgc tggtgtcacc gccggcgcga gggctcgtac
     2581 ccggtcatga tgagccccag ggtccgccgg ctcgtcgtct cacgccactg ggccatccgc
     2641 cgggccagac cctcgggcag gtcgctcgtg tccgtcgtcg tgaaggccga gctgaacgcg
     2701 gcaaacccga ccgtgaaggc gttgatcgac tgccacagct cgaccgcgtc ctcctcgggg
     2761 aagccgctgg agacgaggta gacgaggccg ctctgcgggt cggtgcccac ccgccggccg
     2821 gcgaggggaa gcatgttcgg gtgcgcagcc agcacggcga ggtaggtcgt gaagacgagc
     2881 tccatgaggg cgcgccaatc ctgcgggagg ggctcggcga agaccatctc ggagcgcatc
     2941 aggctcaggg ccccgtcgag gagctcgtcc ttgcccgaca cgtggtggta gagcgaggca
     3001 gcctccacgc cgacgtccgc ggccagccgg cgcatcgtca gcgcctccag acccccggcg
     3061 tcgatgatcg ccagcgcccg ctcgaagagc cgctcccgag tgaccggccg ccgccctgcc
     3121 tgcgctcgtg ccacgacccc tccttgcctc gcctgcgggg ttgacgccca ccggcgtgag
     3181 gtgacaccgt agagtcacct aacaatgtta ggccaaccga tctggtgatg ggcagatgag
     3241 ttcggcgcag acctcggccc caacgcaggc cccgaccaca tcgcaggctc cgagcaccgc
     3301 cgcgcggcgc acagaccggt ggcgaacgct ctacctcatc ggcgcgacgg cggccctcgt
     3361 cggggtcggc ggggctctcc tcgacatcgt gctgacctcg ctgccgggtt ggagacccga
     3421 caccgtcccg cggaccctgg ccggctggtt cggccagctg gacgacaggc cctggct
//
LOCUS       DATAIX010000036         1870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_91426, whole genome shotgun sequence.
ACCESSION   DATAIX010000036 DATAIX010000000
VERSION     DATAIX010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91426"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(415..1380)
                     /locus_tag="VHM65_00630"
     CDS             complement(415..1380)
                     /locus_tag="VHM65_00630"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX2704237.1"
                     /translation="MTEPTKTVLLAAPRGYCAGVDRAVVTVEKALELYGPPVFVRKEI
                     VHNRHVVTTLQDRGAVFVDETDEVPEGATVIFSAHGVAPIVHEEARVRGLRTIDATCP
                     LVTKVHREAVRFADADYDILLIGHEGHEEVVGTAGEAPDHITLVDGPADVATVSVRDP
                     DKVVWLSQTTLSVDETMETVRRLRERFPSLQDPPSDDICYATQNRQLAVRQMAPRCDV
                     VIVVGSRNSSNSVRLVEVALEHGARASRLVDEAAEIDAAWLDGATTVGVTSGASVPEI
                     LVQDVLALLAAHGFATVETVVAAEESLIFSLPQELRADLKGAGKG"
     gene            complement(1499..>1870)
                     /locus_tag="VHM65_00635"
     CDS             complement(1499..>1870)
                     /locus_tag="VHM65_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX2704238.1"
                     /translation="STYAVPGLYFYGSDVVERARALKPSPRGELEITDLNRTYLEEGR
                     LSVAVLPRGTAWLDTGTFDSLHDASSYVRTIEQRQGLKIGSPEEVAWRVGFLTDDELR
                     ARAELLVKSGYGAYLLGLLGS"
BASE COUNT          293 a          646 c          644 g          287 t
ORIGIN      
        1 cgaagtcctc cggcttgtag agccgggcaa gaagcggtga ggcgaggaag gcgaccgcct
       61 gccccccgac cgtgccgacc aagatggtga ggatgccgcg tggcgcgctg actccacctg
      121 ccgacccgtt ctcaccggag ccgaccacga gtgggtcggc gtcaacctgg ccccccagag
      181 ggatgcacca gcgtacgccc gggaccctgg cagcccggac acccgccgct cgcggctgtc
      241 gttcacagcg gaacgtcaac accaccgggt acgtttgtgc cggggcgtcg ggaaggccgt
      301 ttgtgctgtt gaacgtggtc ggtcttgcag acgtgactgg tcttgcagac gggggcgtcg
      361 cactggccga agggcacgcc cccgaatggg ggcactcagc gggtcctccg ggtcttagcc
      421 cttgccggca cccttgaggt cggcgcgtag ctcttgggga agggagaaga tcagggactc
      481 ctcagccgcc acgacggtct cgacggtggc gaacccgtga gcggccaaca gcgcaagcac
      541 gtcttgaacg aggatctccg gcacgctcgc cccggatgtc accccgaccg tcgtcgcgcc
      601 gtccagccag gcagcgtcga tctccgccgc ctcgtcgacg agccggctgg cccgggcgcc
      661 gtgctcgagt gcgacctcga cgaggcgcac cgagttggac gagttgcgcg agcccacgac
      721 gatcacgacg tcgcaccgag gcgccatctg cctcaccgcg agctgtcgat tctgcgtcgc
      781 atagcagatg tcgtcgctcg gcgggtcctg cagcgacggg aagcgctccc gcaggcgccg
      841 caccgtctcc atcgtctcgt cgaccgagag cgtcgtctgg gagagccaca cgaccttgtc
      901 cgggtctcgg acggacaccg tggccacgtc ggcgggcccg tcgaccagcg tgatgtggtc
      961 cggtgcctcc cctgcggtcc cgacgacctc ctcatgcccc tcgtgcccga tgagcaggat
     1021 gtcgtagtcc gcgtcggcga agcgcacggc ctcccggtgg accttggtga ccaaggggca
     1081 cgtcgcgtca atcgtcctga gcccgcggac ccgcgcctcc tcgtgcacga tgggcgccac
     1141 cccatgggca gaaaagataa ccgtcgcccc ttcggggacc tcgtccgtct cgtcgacgaa
     1201 gaccgcaccg cggtcttgca gggtggtgac gacgtgccgg ttgtggacga tctccttgcg
     1261 gacgaagacc ggaggcccat acagctcgag agccttctcg accgtcacca cggcccggtc
     1321 cacccccgca cagtagcccc ggggcgcggc gaggagcacc gtcttggttg gttcggtcac
     1381 cactccatcg taggtcgtgc tgtgggcagc cccgaagcgg ccgccggccc cgcgccgcac
     1441 tgtgcgggat tgtcaggagc actgggccgg attgtcagga aagcactggg ccggattgtc
     1501 aggagccgag cagcccgagc aggtacgcgc catacccact cttgacgagg agctctgcgc
     1561 gcgccctcag ctcgtcgtcg gtgaggaagc cgacccgcca ggcgacctcc tcgggggaac
     1621 cgatcttgag cccctggcgc tgctcgatcg tccggacgta gctcgacgcg tcgtgaaggg
     1681 agtcgaaggt cccggtgtcg agccatgccg ttccacgcgg gagcaccgcc accgagaggc
     1741 ggccttcctc gagataggtg cggttgaggt cggtgatctc gagctcgccg cgaggggagg
     1801 gcttcagggc gcgagcccgc tcgacgacgt cgctcccgta gaagtacagg ccggggacgg
     1861 cgtaggtgct
//
LOCUS       DATAIX010000037         3145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_151069, whole genome shotgun sequence.
ACCESSION   DATAIX010000037 DATAIX010000000
VERSION     DATAIX010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151069"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1516)
                     /gene="hflX"
                     /locus_tag="VHM65_00640"
     CDS             complement(50..1516)
                     /gene="hflX"
                     /locus_tag="VHM65_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX2704239.1"
                     /translation="MLSDRAAALDSDPDLDPGGTSRHGGHDGEQLERDERAALRRVAG
                     LSTELEDVTEVEYRQLRLERVVLAGVWTEGTLEDAENSIRELAALAETAGSTVLEGVL
                     QRRQRPDAATYLGKGKALELRDIVIAEGADTVICDGELAPGQRRALEDVVKVKVIDRT
                     ALILDIFAQHAKSREGKASVELAQLQYLLPRLRGWGESMSRQAGGRVAGGEGIGSRGP
                     GETKIELDRRRINTRMAKLKRDIAHMKTGRDTKRHSRRAHGVPSVAIAGYTNAGKSSL
                     LNRLTGAGVLVQNQLFATLDPTVRRAETSDGRTYTLVDTVGFVRRLPQQLVEAFRSTL
                     EEVGECDLLLHIVDGSHPDPEGQISAVREVLADVGGDRIKEVVVINKADAADPDVLTR
                     LTRHEKHSIVVSARTGEGLEQLRELIGAELPRPDIDVTLVVPYDRGDLLSRLHDEGEV
                     LSTEHTEDGTVVVARIKATLEGEVAPYLVPHGRVAGHR"
     gene            1663..2277
                     /locus_tag="VHM65_00645"
     CDS             1663..2277
                     /locus_tag="VHM65_00645"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX2704240.1"
                     /translation="MPSPPEHYFTATPAGAVGHRMVPVDLAGRSFTVRTASGVFCPDR
                     IDLGTAVLLREAPDPARAGALLDLGCGWGPIALTLGLLSPEADVWAVDVNERALDLVR
                     TNAKTVGLHRFRAALPDDVPGGLRFATIWSNPPIRIGKAVLHSVLQRWLPRLEPSGAA
                     YLVVAKDLGADSLARWVDDSLDGMTCARVATSKGFRVLKVRWAP"
     gene            complement(2310..>3145)
                     /gene="dapF"
                     /locus_tag="VHM65_00650"
     CDS             complement(2310..>3145)
                     /gene="dapF"
                     /locus_tag="VHM65_00650"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX2704241.1"
                     /translation="AGSPWSSLSRMAPHELDLSAGTVRLLCDRRAGIGGDGVIRVVPT
                     EHADEPAVREQAGEARWFMDYRNADGSPAEMCGNGSRVFAAFLRRERLETADEFAIAT
                     RAGAKHVRVEADLIAVDLGPWRLGDEDAAAERGFDALVTAHGAHELSGLSLHVGNPHT
                     VVALPPGISLAQLDLTVAPSVAPAPGHGTNVEFVDPIGHGHIAMRVHERGVGETRSCG
                     TGAAAAALATRWWEGESESNREWTVDVAGGRLRVRALPDHRVELAGPAVLVADGETSL
                     R"
BASE COUNT          405 a         1109 c         1155 g          476 t
ORIGIN      
        1 cccggagggt cggggacggc gaatcccgct gggacaaggc agggttcgct caacggtgac
       61 cagccacccg accatgcggc acgaggtatg gcgcgacctc accttccaga gtcgccttga
      121 tgcgggcgac cacgacggtg ccgtcctcgg tgtgctccgt cgagaggacc tcgccctcgt
      181 cgtgcaggcg gctcagcagg tcgccgcggt cgtacgggac gacgagggtg acgtcgatgt
      241 cgggccgggg cagctcggca ccgatgagct cgcgcagctg ctccagcccc tcgccggtgc
      301 gggccgagac gacgatggag tgcttctcgt ggcgggtgag acgggtgagg acatcggggt
      361 cggcggcgtc ggccttgttg atgacgacga cctctttgat ccggtcgcca ccgacatccg
      421 cgagcacctc gcgcacggcc gagatctggc cctcggggtc ggggtgcgag ccgtcgacga
      481 tgtgcaggag caggtcgcac tcccccacct cctccagcgt cgagcggaac gcctcgacga
      541 gctgctgggg cagccggcgg acgaagccga ctgtgtcgac gagggtgtag gtgcgcccat
      601 cggaggtctc ggcgcgacgc acggtcgggt ccagggtggc gaagagctgg ttctgcacga
      661 ggaccccggc tccggtgagc cggttgagca gggaggactt gcccgcgttg gtatagccgg
      721 cgatggcgac gctcggcacc ccatgggccc gccgagagtg gcgtttggtg tcgcgcccgg
      781 tcttcatgtg ggcgatgtcg cgcttgagct tggccatcct ggtgttgatg cggcgccggt
      841 cgagctcgat cttggtctcg cccggccctc gcgagccgat gccctcgcca ccggcgaccc
      901 ggccaccagc ctgccgcgac atcgactcgc cccagccccg caggcggggc aggaggtact
      961 gcagctgggc cagctcgacc gaagccttgc cctcccggct cttggcgtgc tgggcgaaga
     1021 tgtcgaggat cagcgcggtc cggtcgatga ccttgacctt gacgacgtcc tcgagcgcgc
     1081 gtcgctggcc gggggcgagc tcgccgtcgc agatgacggt gtcggcgccc tcggcgatga
     1141 cgatgtcgcg cagctccagg gccttgccct tgccgagata cgttgcggca tcgggtcgct
     1201 ggcgtcgctg caggacgcct tccagcacgg tggagccggc cgtctcggcg agtgcggcaa
     1261 gctcgcggat cgagttctcg gcgtcctcga gcgtgccctc ggtccagact ccggcgagga
     1321 cgacgcgctc gagccggagc tggcggtact cgacctcggt gacgtcctcg agctcggtcg
     1381 agagcccggc gacacgacgc agcgccgcgc gttcgtctcg ttcgagctgc tccccgtcat
     1441 gcccgccgtg cctggacgtg ccgccggggt cgaggtccgg gtcgctgtcg agggcggcgg
     1501 cgcggtcgga gagcacaggt gatcgtcgtg tcatatcccc tctaggctct caccttcgcc
     1561 cggtaccccg cgacgcaatt gacccccggc gccggaccgg ggccatccgt ccgggcgggt
     1621 cgggctcctc ccaggggcgt cctggtcgct agcgtgtcct cgatgccttc cccacccgag
     1681 cactacttca cggcaacgcc ggccggcgcc gtcgggcacc ggatggtgcc cgtcgacctc
     1741 gccgggcggt ccttcacggt gcggacggcg tccggggtgt tctgccccga ccgcatcgac
     1801 ctgggcacgg ccgtgttgct gcgcgaggcg ccggacccgg cccgagccgg ggcgctgctc
     1861 gacctcggct gcggctgggg gccgatcgcg ctgacgttgg ggctgctctc gcccgaggcg
     1921 gacgtgtggg cggtcgacgt caacgagcga gccctcgacc tcgttcgcac caacgcgaag
     1981 accgttggcc tgcaccgctt ccgggcagcc ctgcccgacg acgtgcccgg cgggctgcgg
     2041 ttcgcgacga tctggtccaa cccgccgatc cggatcggca aggcggtcct gcactccgtg
     2101 cttcagcggt ggctgcctcg gctggaaccg agcggcgcgg cgtacctcgt ggtggcgaag
     2161 gacctcggtg cggactcact ggctcgctgg gtcgacgact cgcttgacgg tatgacgtgc
     2221 gccagggtcg cgacgagcaa gggcttccgg gtgctgaagg tccgctgggc gccctgagcc
     2281 ggctccgggt ggcccgcgga gtgccccagt cagcgaagcg aggtctcgcc gtcggcgacg
     2341 aggacggccg gcccggcgag ctcgacgcga tggtccggga gggcccgcac gcggagccgc
     2401 ccgcccgcga cgtcgacggt ccactcgcgg ttggactccg actcgccctc ccaccaccgg
     2461 gtcgcgaggg ctgccgcggc cgcgcccgtg ccgcaggacc gggtctcgcc gaccccgcgc
     2521 tcatggacgc gcattgcgat gtgtccgtgc ccgatcgggt cgacgaactc gacgttggtg
     2581 ccatggcccg gcgcgggtgc gaccgaaggg gcgacggtga ggtcgagctg ggccaggctg
     2641 atgccagggg ggagagcgac gaccgtatgc gggttgccga cgtgcaggct cagccccgac
     2701 agctcgtggg ccccgtgggc cgtcacgagg gcgtcgaaac cgcgctctgc tgcagcgtcc
     2761 tcgtcaccca gccgccacgg cccgaggtcg acggcgataa ggtcggcctc gacgcgcacg
     2821 tgcttggccc cggcgcgcgt ggcgatcgcg aactcgtctg ccgtctcgag ccgctcacgg
     2881 cgcagaaagg ccgcgaagac ccgcgacccg ttgccgcaca tctcggccgg cgagccatcg
     2941 gcgttgcggt agtccatgaa ccaccgtgcc tcgccggcct gctcacgaac cgctggctcg
     3001 tccgcgtgct cggtgggcac gacgcggatc accccgtccc cgccgatccc ggcccgccgg
     3061 tcacacagca gccggacggt cccggcgctc aggtcgagct cgtgcggcgc catacgggac
     3121 aatgacgacc atggcgagcc tgcgt
//
LOCUS       DATAIX010000038         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_32162, whole genome shotgun sequence.
ACCESSION   DATAIX010000038 DATAIX010000000
VERSION     DATAIX010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32162"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VHM65_00655"
     CDS             <1..409
                     /locus_tag="VHM65_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704242.1"
                     /translation="RMPHPNPASGASGHDPVLPGSRRVELQRAMTAGTGVVRTAGSLA
                     STAATLAALSGEAQAASATPGPGGWETTNLLQLGQALTAVATLREETRGGHVRLDFPD
                     RDDRRWNGHLRCRRSASGTLVTSYQTTPVQDLT"
     gene            441..1313
                     /gene="nadC"
                     /locus_tag="VHM65_00660"
     CDS             441..1313
                     /gene="nadC"
                     /locus_tag="VHM65_00660"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX2704243.1"
                     /translation="MSTGFPRDAAAVLVATALREDLGPDGLDVTTRATIPVHQVRTAH
                     IVARADGVLAGTPVIEEVLDQVAALVGAPEPVVTVLLGDGFPVTRGDVVATVSGPTQV
                     ILTAERTILNIVCRLSGVATHTRRWADQLEGTGASVLDTRKTTPGMRLLEKYAVRAGG
                     GTNKRMGLYDVAMIKDNHKLAAGSLTAAFDAVRAAFPQAPIQVEVTTADEAREAVAAG
                     ARFVMCDNMSPELLAATVAAIRHTGEAVEVEATGGLTLATVREYARTGVDYLSVGGLT
                     HSSPILDLALDLVG"
     gene            1358..>1538
                     /locus_tag="VHM65_00665"
     CDS             1358..>1538
                     /locus_tag="VHM65_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704244.1"
                     /translation="MTALPPADLPAHEEPPDDDLPEQMRVRRDKRAQLLTAGHAPYPV
                     WVDRTHTLAQVREQWG"
BASE COUNT          221 a          584 c          546 g          187 t
ORIGIN      
        1 acggatgccg cacccgaacc ccgccagcgg cgcgagcggc cacgacccgg tgctgccagg
       61 gtcgcgccgg gtcgagctgc agcgggcgat gacggccggg accggggtcg tgcgcacggc
      121 cggctcgctc gcgtcgaccg cggcgaccct ggccgccctg agcggcgagg cgcaagcagc
      181 ctcggcgacg ccgggccccg gcgggtggga gacgacgaac ctgctccagc tggggcaggc
      241 cctgaccgct gtggcgaccc tgcgcgagga gacccgcggc gggcacgtgc gcctcgactt
      301 ccccgaccgt gacgaccgcc gctggaacgg ccacctccgc tgccggcgta gcgcgagtgg
      361 aacgctcgtg acgtcatacc agaccacgcc ggtccaggac ctcacatgag tcaggacctc
      421 acatgagtac cggctctcac atgagtaccg gcttccccag ggacgccgcg gcggtccttg
      481 tcgcgaccgc gctgcgcgag gacctgggcc ccgacgggct cgacgtgacg acgcgggcga
      541 cgatcccggt tcaccaggtg cgcacggcgc acatcgtggc ccgggccgac ggcgtcctcg
      601 ccggaacgcc ggtcatcgag gaggtgctcg accaggtcgc cgcgctcgtc ggtgccccgg
      661 agcccgtcgt gacggtcctg ctcggcgatg gcttcccggt cacccggggc gacgtggtgg
      721 cgacggtctc ggggccgacc caggtcatcc tcacggccga gcggaccatc ctcaacatcg
      781 tctgccgcct ctccggcgtg gccacgcaca cccggcgctg ggccgaccag ctcgagggca
      841 cgggcgcgtc cgtcctcgac acgcgcaaga cgaccccggg aatgcggctg ctggagaagt
      901 acgccgtgcg agccgggggc ggcaccaaca agcgcatggg cctctacgac gtggccatga
      961 tcaaggacaa ccacaagctc gccgccgggt cgctgacggc cgcgttcgac gcggtgcgcg
     1021 cggcgttccc gcaggcgccg atccaggtcg aggtgacgac ggccgacgag gcgcgcgagg
     1081 ccgtggcggc cggggctcgc ttcgtcatgt gcgacaacat gtcacccgag ctgctggccg
     1141 ccactgtcgc ggccatccgt cataccggcg aagccgtcga ggtcgaggcg accggcgggc
     1201 tcacgctggc gaccgtgcgg gagtatgccc ggaccggtgt ggactacctc agcgtcggag
     1261 ggctcaccca ttcctcgccg atcctcgacc tggcgctcga cctcgtcggc tgaccacccg
     1321 ccaagggcat ccctgcggcc tcggtaggct tgcccccgtg accgcgttgc ccccagccga
     1381 cctgcctgcc cacgaggagc cgcccgacga cgacctgccc gagcagatgc gggtccgccg
     1441 ggacaagcgg gcccagctgc tcaccgccgg ccacgcgcca taccccgtct gggtcgaccg
     1501 gacccacacc ctcgcccagg tgcgcgagca gtgggggc
//
LOCUS       DATAIX010000039         2933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_91725, whole genome shotgun sequence.
ACCESSION   DATAIX010000039 DATAIX010000000
VERSION     DATAIX010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91725"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VHM65_00670"
     CDS             complement(<1..701)
                     /locus_tag="VHM65_00670"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX2704245.1"
                     /translation="MHSHFNGLKTAVLFGGIFAVLLAMGSLISGGRFLWVFALLGVAM
                     TFYGYWNSDKLAIRAMHARPVSEAEAPAMYRIVRELSTAAGKPMPRLYISPTEAPNAF
                     ATGRNPANAAVCCTEGILALLTERELRGVLGHELMHVYNRDILTGSVAAAIAGIITSV
                     SQMAMFAGMFGGGSSDENRPNPIALLLMALMAPLAAGVIQMAISRTREYDADEDGAKL
                     TGDPIALASALRKLEL"
     gene            complement(741..2204)
                     /locus_tag="VHM65_00675"
     CDS             complement(741..2204)
                     /locus_tag="VHM65_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX2704246.1"
                     /translation="MTVSLDLSVLLVVLAPIIGAILVLVADLVAPRQQRAHYAIAFIS
                     LLLGVYGTLATLAADPGNTAKTLCLDAVNCLYEVNAVSATLQLAALLGAMAVLALGWP
                     LERPQQADRTSTQIVLLLTATSGAVGVAGARDLASWLVLLELATVPVVVLVAIPGTRR
                     ALDGAMQLLTTSLVSFGLLVLGAALWYAATGSALLRPNAVLAVDTSAQRALLTLSVVL
                     ILSGIGFKLSLAPFHAWTPATYAGAPLPIATFLAAVSKVAALAAVVVVMQALAALGGS
                     ALATIAVLAILSMTIGNVVALRQNDVVRLLAWSTVGQAGWVIMPLVSISPSGVKAASG
                     YLLGYVVATIVAFTVVTIVARGAQSQRGRAPYQLDAYRGLLRARPGLGGALALALTSL
                     AGLPPGLLGLVTKVLALRPVLAEGWWVLAVLAAVNVVIGVAVYVRWFRVLLEPLPAPA
                     LPSSVRTHPSHAIALATSATLLLVLSLAPQSMLGLFG"
     gene            complement(2201..>2933)
                     /locus_tag="VHM65_00680"
     CDS             complement(2201..>2933)
                     /locus_tag="VHM65_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX2704247.1"
                     /translation="PTVPQGFASVASVLAGVGVVGIIWGGLACLVERDLKRLIAYSSV
                     AHMGFVALALASGTETGVQAALFANIAHGIVSALLFFLVGALKDRWLTADLDTLPGAL
                     RETSPRFGFAFVVGFAGALGLPGLVGFWGEFLAVYAAWNPMSGRPVGFFQACAVVAVI
                     GMTIAASYSLRVLRVIWAGESTEPGRGDARGLEWAVVATLIVAVIGAGVLPRPLLAVT
                     AADVTTLVSGSTAAAAGGTAAKATP"
BASE COUNT          512 a          983 c         1110 g          328 t
ORIGIN      
        1 agctcgagct tgcgcagcgc cgaggcgagg gcgatcgggt caccggttag cttcgcgccg
       61 tcctcgtccg cgtcatactc acgggtgcgg ctgatcgcca tctggatgac ccccgccgcg
      121 agcggcgcca tgagcgccat gagcagaagg gcgatcgggt tgggccggtt ctcatccgat
      181 gacccgccgc cgaacatccc cgcgaacatc gccatctggg agaccgaggt gatgatcccg
      241 gcgatggccg ctgcgacgga cccggtgagg atgtcgcggt tgtagacgtg catgagctcg
      301 tggccgagga ccccacgcag ctcacgctcg gtgagcagag cgaggatccc ctcggtgcag
      361 cagaccgctg cgttggccgg gttgcggccg gtggcaaagg cgttcggggc ttcggtcggt
      421 gagatgtaca ggcgtggcat cggcttgccg gcagccgtcg agagctcacg cacgatccgg
      481 tacatcgccg gggcctcggc ctcgctgacc ggccgcgcgt gcatggcgcg gatcgcgagc
      541 ttgtcggagt tccagtagcc gtagaaggtc atcgcgacac cgagcagggc gaagacccag
      601 aggaagcgcc cgccggagat gagcgagccc atcgcgagca ggacggcgaa gatcccgccg
      661 aagaggaccg cggtcttgag cccgttgaag tggctgtgca tgtcagcacc aacgcgaggc
      721 cgggccccga cgttcccggc tcagccgaag aggccgagca tcgactgcgg cgccaggctc
      781 aggacgagga gcagcgtcgc gctggtcgcg agggcgatcg cgtggctcgg gtgggtccgc
      841 accgacgacg gcagcgcggg cgccggcagc ggctccagca gcaccctgaa ccaccgcacg
      901 tagaccgcga cgccgatgac gacgttgacc gccgccagga ccgcgaggac ccaccagccc
      961 tcggccagga ccgggcgcag cgcgagcacc ttggtgacca gcccgagcag ccccggcggc
     1021 aggcccgcga gcgaggtgag cgccagggcc agggccccgc ccaagccggg ccgcgcacga
     1081 agcagacccc gataggcgtc gagctggtat ggcgcgcgcc cccgctggct ctgcgctccc
     1141 cgggcgacga tggtcacgac cgtgaaggcg acgatggtgg cgacgacgta gccgagcagg
     1201 tagccgctgg ccgccttgac cccgctcggg gagatactca cgaggggcat gatgacccag
     1261 ccggcctggc cgacggtcga ccaggcgagc agccggacga cgtcgttctg gcgcagcgcg
     1321 acgacgttgc cgatggtcat gctgaggatg gcgagcacgg cgatggtggc cagggccgag
     1381 ccgcccagcg cggccagagc ctgcatcacg acgacgactg cggcgagggc ggcgaccttg
     1441 gacacggcgg cgaggaaggt cgcgatcggg agcggggcgc cggcatacgt cgcgggggtc
     1501 cacgcatgga agggggccag ggacagcttg aaaccgatcc ctgagagaat cagcacgacc
     1561 gagagcgtca gcagggcccg ctgagccgag gtgtcgacgg cgagaacggc gttggggcgc
     1621 agcagcgccg acccggtcgc ggcataccag agcgcggcgc cgaggacgag caggccgaac
     1681 gacacgagcg aggtcgtgag cagctgcatc gccccgtcga gggcacggcg cgtgcccggg
     1741 atcgcgacga ggacgacgac gggcaccgtg gccagctcga gcagcacgag ccaggaggcg
     1801 aggtcgcggg ctccggccac cccgacggca cccgaggtgg ccgtcagcag caggacgatc
     1861 tgggtgctcg tccggtcggc ttgctgcggg cgctccaacg gccacccgag cgccaggacg
     1921 gccatcgccc cgagcagggc ggccagctgc agggtcgcgc tcaccgcgtt gacctcgtag
     1981 aggcagttga ccgcatcgag acacagcgtc ttcgccgtgt tgccggggtc ggccgcgagc
     2041 gtcgccagag tcccatagac gccgaggagc aggctgatga acgcgatggc gtagtgggcc
     2101 cgctgctggc gcggcgcgac gaggtcggcg acgaggacga ggatggcgcc gatgatcggc
     2161 gcgaggacca cgagcaggac gctcaggtcg aggctgacgg tcatggcgtg gccttcgcgg
     2221 cagtgccgcc ggcggcagcg gcagtgctcc cggagacgag cgtggtgacg tccgcggcgg
     2281 tcaccgcgag caggggcctc ggcaggacgc cggcaccgat gacggcgacg atgagcgtcg
     2341 cgacgacggc ccattcgagg ccgcgcgcgt caccgcggcc cggttcggtg gactctccgg
     2401 cccagatgac gcgcaggacg cgcagcgagt atgacgccgc gatcgtcatc ccgatgaccg
     2461 cgacgaccgc gcacgcctgg aagaacccga ccgggcgtcc gctcatcggg ttccacgcgg
     2521 cgtacacggc gaggaactca ccccagaacc cgaccagacc cggcaggccg agcgcaccgg
     2581 cgaaaccgac gacgaaggcg aacccgaacc ggggtgacgt ctcgcgcagc gccccgggga
     2641 gagtgtcgag gtcggcggtg agccagcggt ccttgagggc tccgacgagg aagaagagca
     2701 gggcggacac gatgccgtgg gcgatgttgg cgaagagcgc cgcctgcacc ccggtctcgg
     2761 tcccgctcgc gagcgcgaga gccacgaagc ccatgtgcgc gaccgaggag taggcgatga
     2821 gtcgcttgag gtcacgctcg acgaggcagg ccaggcctcc ccagatgatc ccgacgactc
     2881 ccacgcccgc gagcacggac gccaccgagg caaacccctg cggcacggtc ggc
//
LOCUS       DATAIX010000040         5637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_91809, whole genome shotgun sequence.
ACCESSION   DATAIX010000040 DATAIX010000000
VERSION     DATAIX010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91809"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..922
                     /gene="proC"
                     /locus_tag="VHM65_00685"
     CDS             83..922
                     /gene="proC"
                     /locus_tag="VHM65_00685"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX2704248.1"
                     /translation="MANGDTAPPTATHTTAIFGAGVMGETLLSGLLRAGRSPQGLVIA
                     ERRADHAAMLRDRYGVRVVSNIEAAELADTAVLVVKPQDMAGLLDEIHDHIAPGNLVV
                     SLAAGITTDFLQARLPQGSSVVRVMPNTPALVDQGMAAISPGQHCTESHLAEAEALLT
                     SCGKVVRVPEHYQDAVTAISGSGPAYIFYVVEAMIEAGVVLGLPRATSTELVVQTLYG
                     AATMLKETGQHPSILREQVSSPGGTTVAALRQLDDHKVRAAFVNAIEAAAARSSELAS
                     GHV"
     gene            1016..1546
                     /locus_tag="VHM65_00690"
     CDS             1016..1546
                     /locus_tag="VHM65_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2704249.1"
                     /translation="MTEIDVRRLSEDDWQDYRAVRLDALRESPDAFVATHAEESALDE
                     AHWRDRLRRSVRLVASVEAKDIGVVSVGRAGHTGDGFSDADGAVGELFGLWVHPSLRG
                     QGVAMQLVNEGAAQARDAGSTHLCYWVGTDNGPAVAFASGIGFRPTDHRRPMRGAAHE
                     DEEEIAMILPLGADRG"
     gene            complement(1543..2208)
                     /locus_tag="VHM65_00695"
     CDS             complement(1543..2208)
                     /locus_tag="VHM65_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX2704250.1"
                     /translation="MARGRGDDDVVVVGLGSFGVAVATELTSLGNRVTVLERQRERAE
                     RLGRGFHRVIVADASDPATLEEAHAPEAASAVVAIGSSVEASVLTAANLVDLGVRSIW
                     AKASSPRHARILERLGVHKVIFPEAESGRRVAHLLDGRMLDYIEFDDGFAVVKMRPPS
                     ELIGFTLGESRVRSKYGVTVVGVKRSAEAFTYATPETKVTNRHEILVSGPVKLIERLA
                     NRP"
     gene            2343..3470
                     /locus_tag="VHM65_00700"
     CDS             2343..3470
                     /locus_tag="VHM65_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HEX2704251.1"
                     /translation="MSPVRLDLTARLCESFGIFEHVEVEVVSPEVAEDAVLSTVHDLA
                     YIAAVKAASADPDNADPSHGLGTDDDPAFRGMHEASARIAQGTLDVCRAVWQDEAEHG
                     VNFCGGMHHAMPGSASGFCIYNDAAVGIKWLLDHGAERVAYVDIDVHHGDGVERVFWN
                     DPRVLTVSIHETGRALFPGTGWASDIGGAGAQGYAANVSLPPGVGDGAWLRAFHSSVL
                     PLVRAFRPQILVTQHGADTHAQDPLAHLALTVDAQRMAADELHRLSHEVCAGRWVALG
                     GGGYEVVDVVPRTWTHLTAIAAHHPIRVGTEVPDDWRHHVLTSTGRPGPTHMGDIDPS
                     EGSPWWRSWDMGYDPASEVDRAIMASRQALFPYHGLDVWFD"
     gene            3633..3833
                     /locus_tag="VHM65_00705"
     CDS             3633..3833
                     /locus_tag="VHM65_00705"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2704252.1"
                     /translation="MTTERQLAQVRFLTVAEVASIMRVSKMTVYRLVHGGDLPAVRVG
                     RSFRVPEDAVHTYLETSYVDTA"
     gene            3989..4087
                     /locus_tag="VHM65_00710"
     CDS             3989..4087
                     /locus_tag="VHM65_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HEX2704253.1"
                     /translation="MGSVIKKRRKRMAKKKHRKLLRKTRHQRRNRK"
     gene            4275..5372
                     /locus_tag="VHM65_00715"
     CDS             4275..5372
                     /locus_tag="VHM65_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2704254.1"
                     /translation="MSRVVLITGVSRYLGGAYARSLSANPSIERMIGIDVIPPPHGIG
                     RAEFVRADIRNPMIGKIISQAGVDTVVHLNVIATPTNAGGRVSQKEINVIGTMQLLAA
                     CQKATTVRRLVVKSSAAVYGSSPRDPAMFSEDMSPKSLPRSGFGKDSVEVEGYVRGYS
                     RRRPEVEITMLRFANIIGPDIRTSITDYFSLPVIPIPLGYDARLQLCHERDAVEALIM
                     ATLGPEIGICNIAGDGIITVTQAAAIVGRPVLPVPLESAGMLGNLLKRAGLADFSPDQ
                     MNFLAYGRGLDTTKMREKLGFTPAFTTRQTFEDFARNVGPAVPGLTTVGGLLANAAQL
                     VLSTATSAVSAVGSLVPHGDPGHRANGKDRR"
     gene            5369..>5637
                     /locus_tag="VHM65_00720"
     CDS             5369..>5637
                     /locus_tag="VHM65_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704255.1"
                     /translation="MTTPSPRPKAVSASAQALALGAARASGERARRSSTPLLGPQAAP
                     AVAAQPLAGDSRRTTAVSPVIARGAATSAASTAAAGAVPTTHRPVP"
BASE COUNT          831 a         2059 c         1930 g          817 t
ORIGIN      
        1 gcgtaccgtg cttgcgcggg gcagcgaggc gtgcggctta ccgcagccgc ccgcggcccc
       61 ggcgctccgc catactggcc gcatggcgaa cggcgacacc gcacccccca ccgcgaccca
      121 cacgaccgcg atcttcggcg ccggcgtcat gggggagacc ctgctctccg gcctcctgcg
      181 cgccgggcgc tcaccgcagg ggctcgtcat cgccgagcgc cgggccgacc acgccgcgat
      241 gctgcgcgac aggtacggcg tccgggtcgt gagcaacatc gaggccgcag agctcgccga
      301 cacggccgtc ctcgtcgtca agccgcagga catggccggc ctgctcgacg agatccacga
      361 ccacatcgcc ccgggcaacc tcgtcgtctc cctcgcggcc ggcatcacga ccgacttcct
      421 ccaggcccgg ctgccgcagg ggtcctcggt cgtgcgggtc atgcccaaca cccccgccct
      481 cgtcgaccag ggcatggccg cgatctcccc cggtcagcac tgcaccgagt cgcacctcgc
      541 cgaggccgag gccctgctca cgtcgtgcgg caaggtcgtg cgggtgcccg agcactacca
      601 ggacgcggtc actgcgatca gtggaagcgg tccggcatac atcttctacg tcgtcgaggc
      661 gatgatcgag gcgggtgttg tgctcggact accgcgggcc acctcgaccg agctcgtcgt
      721 ccagacgttg tatggcgccg cgacgatgct caaggagacg ggccagcatc cgagcatcct
      781 gcgcgagcag gtgtcctctc cgggtggcac caccgtcgcg gcgctgcgcc agctcgacga
      841 ccacaaggtc cgggccgcgt tcgtcaacgc gatagaagcg gcggccgcgc gctcgagcga
      901 gctcgcgtcg ggccatgtgt gaggtcgggg cgcgctgacg gcagggctgt ctcccggcgg
      961 tgagctcggc ggcggggatc gctattcgcc ggcgcgtcgt gcaaggctag ggcgcatgac
     1021 cgagatcgat gtgcggcggc tgagcgagga cgactggcag gactatcgcg cggtgcgcct
     1081 cgacgcgctg cgcgagtccc ccgacgcctt cgtggccacc cacgccgagg agtctgcact
     1141 cgacgaggcc cactggcgcg accgtctgcg tcgttcggtg cggctcgtcg cgtcggtcga
     1201 ggccaaggac atcggcgtcg tgagcgtcgg ccgggccggt cacaccggtg acgggttcag
     1261 cgacgccgac ggcgccgtcg gtgagctgtt cgggctgtgg gtccacccga gcctgcgcgg
     1321 ccagggcgtc gccatgcagc tggtcaacga aggggccgcc caggcgcgcg acgcggggag
     1381 cacccacctg tgctactggg tcggcaccga caacggcccg gccgtggcct tcgcgagcgg
     1441 catcggcttt cggcctaccg accaccggcg cccgatgcgc ggcgcggccc atgaggacga
     1501 ggaggagatc gccatgatcc tccccctggg cgccgaccgc ggctaggggc ggttagccag
     1561 gcgttcgatg agcttgaccg gcccgctcac gaggatctcg tggcggtttg tgaccttggt
     1621 ctcaggggtc gcataggtga aggcttccgc agaccgcttc acgccgacga ccgtcacccc
     1681 gtacttcgac cggacgcggc tctcaccgag ggtgaagccg atcagctcgc tcggcgggcg
     1741 catcttgacg accgcgaacc cgtcgtcgaa ttcgatgtag tccagcatcc ggccgtcgag
     1801 cagatgcgcg acccgacggc ccgactcggc ctccgggaag atgaccttgt ggacaccgag
     1861 gcgctcgagg atgcgggcgt gcctgggaga gctggccttg gcccagatcg agcgcacgcc
     1921 caggtcgacg aggttcgcgg cggtgagcac cgacgcctcg accgaggacc cgatggcgac
     1981 gaccgccgac gcggcctctg gggcgtgggc ctcctccagg gtcgccgggt cgctggcgtc
     2041 ggccacgatg acccggtgga aacctcggcc caagcgttcg gcgcgctcgc gctggcgctc
     2101 gagcacggtg acccggttgc cgagcgaggt cagctccgtg gcgacagcca ctccgaagct
     2161 gcccagcccg acgacgacga cgtcgtcgtc gccacggcct ctggccatcg ttgccctccc
     2221 gtgctccgta acgcgccgtt gttcggtacg gtccaatcat gccgccttct catccccgcc
     2281 ctcaggtgag ggtcatctgg gacccaggct tcacttcgta cgacttcggg ccctcgcacc
     2341 cgatgtcgcc ggtgcgcctg gacctgaccg cgcggctgtg cgagtcgttc gggatcttcg
     2401 agcacgtcga agtcgaggtc gtctcacccg aggtggccga ggacgccgtt ctcagcacgg
     2461 tgcacgacct ggcctacatt gccgccgtca aggcagcctc ggccgacccg gacaacgccg
     2521 atccgagcca cgggctcggg accgacgacg acccggcctt ccggggcatg cacgaggcca
     2581 gcgcgcggat cgcgcagggc accctcgacg tctgccgggc cgtctggcag gacgaggccg
     2641 agcacggcgt gaacttctgc ggggggatgc accacgcgat gcccggttcg gcgtcggggt
     2701 tctgcatcta caacgacgcc gccgtcggga tcaagtggct gctcgaccat ggcgccgagc
     2761 gcgtcgccta tgtcgatatc gacgtccacc acggggacgg tgtcgaacgg gtcttctgga
     2821 acgacccccg cgtgctcacc gtatcgatcc acgagaccgg ccgagcgctc ttccccggca
     2881 ccgggtgggc gtccgacatc ggtggcgccg gcgcgcaggg gtatgccgcc aacgtgtccc
     2941 tgccgcccgg ggtcggcgac ggggcctggc tgcgggcgtt ccactccagt gtgctgccgc
     3001 tcgtgcgcgc gttccgtccg cagatcctgg tcacccagca tggtgccgac acccacgcgc
     3061 aggatcccct cgcccatctc gccctcaccg tcgacgccca gcggatggcg gcggacgagc
     3121 tgcaccggct ctcgcacgag gtctgcgccg gccggtgggt cgccctcggc gggggcggct
     3181 acgaggttgt cgacgtcgtc ccccgcacgt ggacccacct cacggcgatc gcggcccacc
     3241 atccgatccg cgtcggcacg gaggtccccg acgactggcg ccaccacgtc ctgacctcga
     3301 ccggccgccc tggcccgacc cacatgggcg acatcgaccc gtccgaaggc agcccgtggt
     3361 ggcgctcctg ggacatgggc tacgaccccg ccagcgaggt cgaccgcgcc atcatggcca
     3421 gccgccaggc gctcttcccc tatcacggcc tggacgtctg gttcgactga gtccctccgg
     3481 tgcggcgtgt cggctggact tcggggaaac ttctgtgatc gagcgcttcc cctcgcgtca
     3541 catgagcccc tagtgttgct attcgcacgc acgtaggccc ccgccggagg ggaagccggc
     3601 cctgcgcggg tgctagagga tcgaggagca ccatgaccac agagcgtcag ctcgcgcagg
     3661 tgcggttcct gaccgtcgcc gaagtcgcct cgatcatgcg cgtgtccaag atgacggtgt
     3721 accggcttgt ccacggaggc gacctgccgg cggtgcgagt cggacggtcc ttccgggttc
     3781 cggaggacgc cgttcacacg tatctggaga cgtcatacgt cgacacggcg tgacgtcgag
     3841 cggcccacga cccccggctt tcgcgtcgta tgaccggggg cggtaggctc agcggtcagc
     3901 atccaggagg cagctgccct ctggccgcat caccttccat cgcggggccc ggcgtggccg
     3961 gaccccacac cagcacacga ggacggccat gggttctgtc atcaagaagc ggcgcaagcg
     4021 gatggcgaag aagaagcacc gcaagctgct gcgcaagacg cggcaccagc gtcgcaaccg
     4081 gaagtagcac gacccgccac gatcgcgcag cgcgcaaggg ccggcccccg tgccggccct
     4141 tgcgcgtctc cggcagaggc gccgtggcgg gtaccgtgat ggtgacgacc gtcaccggga
     4201 ggcggcacgt gacacgtcgg taggatcgac agcagcgggt ccggtgaggg tctgtgcaga
     4261 ccgggaggtg gatcgtgtcg cgggtcgtcc tgatcactgg ggtctcgcgg tacctcggtg
     4321 gcgcctatgc ccgatccctg agcgcgaacc cctcgatcga gcgcatgatc gggatcgacg
     4381 tcatcccccc accgcatggc atcgggcgcg ccgagttcgt ccgcgccgac atccgcaacc
     4441 cgatgatcgg caagatcatc agccaggccg gagtcgacac cgtcgtccac ctgaacgtca
     4501 tcgcgacgcc caccaacgcg ggcgggcggg tctcccagaa ggagatcaac gtcatcggga
     4561 cgatgcagct gctcgcggcc tgccagaagg ccaccacggt ccggcggctc gtggtcaagt
     4621 cctccgcggc cgtctacggc tcgtccccgc gagaccccgc gatgttcagc gaggacatga
     4681 gcccgaagtc tttgccgcgc agcggtttcg gcaaggactc cgtcgaggtc gaggggtatg
     4741 tccgcggcta ctcgcggcgc cgtcccgagg tcgagatcac gatgctgcgc ttcgccaaca
     4801 tcatcggccc cgacatccgg acctcgatca cggactactt ctcgctgccg gtgatcccga
     4861 tccccctcgg gtatgacgcg cgcctgcagc tctgccatga acgcgacgcg gtcgaggcgc
     4921 tcatcatggc gacgctgggc cccgagatcg gcatctgcaa catcgccggt gacgggatca
     4981 tcacggtgac ccaggccgcg gcgatcgtcg gacgcccggt cctgcccgtc ccgctggagt
     5041 cggccggaat gctcggcaac ctgctcaaac gggccggcct cgcggacttc tcgccggacc
     5101 agatgaactt cctcgcctac ggccgcgggc tcgacacgac caagatgcgc gagaagctcg
     5161 gtttcacacc tgcgttcacg acccggcaga ccttcgagga cttcgcccgc aacgtcggac
     5221 ccgccgtgcc ggggctcacc acggtgggtg gcctgctcgc caacgctgcg cagctggtgc
     5281 tgagcacggc gacctcggcg gtctcggccg tcggttcgct cgtgccgcac ggcgaccccg
     5341 ggcacagggc caacgggaag gaccgccgat gacgaccccc agtccgcggc cgaaggccgt
     5401 cagcgcctca gcccaggccc tcgcgctcgg ggcagcccgg gcatcggggg agcgcgcccg
     5461 gcgctccagc acgccactgc tcggaccgca ggccgccccg gccgtggccg cccagcccct
     5521 ggccggcgac tcacgccgga ccaccgcggt gtccccggtc atcgcgcgcg gagccgcgac
     5581 gagcgcggcg tccacggcgg cggccggcgc ggtcccgacc acccaccgac cggtgcc
//
LOCUS       DATAIX010000041         2242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_62101, whole genome shotgun sequence.
ACCESSION   DATAIX010000041 DATAIX010000000
VERSION     DATAIX010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62101"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VHM65_00725"
     CDS             <1..528
                     /locus_tag="VHM65_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEX2704256.1"
                     /translation="MWYLNRGTGLVLLVLFTLTTVGGVLSTSRATPRWWPRFLTQGLH
                     RSLGLLSVTLLALHVVTAVVDEFVDIRWWQALLPFGATYKAAFLSLGTLALDLTLAVV
                     VTSLLRGRLSVGAWRAVHLTSYVAWLVAVVHAWGIGTDSSLPLGRGVLIGCGGLVGLA
                     ITARVAILRHEEVLV"
     gene            525..1847
                     /locus_tag="VHM65_00730"
     CDS             525..1847
                     /locus_tag="VHM65_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX2704257.1"
                     /translation="MTARTAERTGPVAAGAHPGLASGSDRSPRAMDGPVLLAHLGDGP
                     SLGAHRERFGALALPRIEALVALAREAQVRGRGGAGFPFAVKLEAVAKGRRPVVVVNV
                     AEGEPASWKDTALATLVPHRVLDGALVAAQALGVREIHVVTPSERTAVGAALRHAVDE
                     RRAAGDRMRFTFHLAAPGFVSGQARAVLELMAGRPNLPVTAWEPEAMSGHRGRPTLLS
                     NAETYAHLAMVVRLGAAGCARFGTADEPGTTLLTIDGDGPSPLVREVAYGAPWSAVLS
                     EAELGQDVILGGYHGTWAPAGALRALSVSRAQLAAAGLALGAGVVLPQAAGTCPVDRT
                     AAIVGFLASRSARRCGPCLHGLPALAAALGTWSQLGGFAEPVARVEQLLTLVERRGAC
                     AHPDGTARLVRSLLVALPEEVAAHQRGRCAVTAGGAATVQRRSAPVSS"
     gene            1844..2056
                     /locus_tag="VHM65_00735"
     CDS             1844..2056
                     /locus_tag="VHM65_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX2704258.1"
                     /translation="MTTAVTHRLRVDWPLCTARGLCSELLPEAVALDEWGYPVVTSDI
                     GDDLVADARAAVKACPQRALRLVPRN"
BASE COUNT          252 a          815 c          823 g          352 t
ORIGIN      
        1 atgtggtacc tcaaccgggg gaccggcctc gtcctgcttg tcctgttcac cctgacgacc
       61 gtcggcggcg tgctgtcgac gtcgcgggcg acgccccgct ggtggccacg gttcctcacg
      121 caggggctgc accgcagcct cggcctcctc tcggtgaccc tgctcgctct gcacgtggtg
      181 acggccgtcg tcgacgagtt cgtcgacatc cggtggtggc aggcgctgct gcccttcggc
      241 gcgacataca aggcggcctt cctctccctc gggaccctcg ccctcgacct cacgctcgcc
      301 gtcgtcgtca cgagcctgct gcgtggccgg ctcagcgtcg gagcgtggcg cgcggtccac
      361 ctgacctcgt atgtcgcgtg gctcgtcgcg gtcgtccacg cgtggggcat cgggaccgac
      421 tcgtccctgc cgctcggtcg cggggtgctg atcgggtgcg gtggcctcgt ggggctcgcc
      481 atcaccgccc gagtcgcgat cctgcgccac gaggaggtgc tggtatgacg gcccgcacgg
      541 ccgagcgcac cggaccggtc gctgcaggcg cgcacccggg actggcctcg gggtccgacc
      601 ggagcccgcg ggccatggac ggccccgtcc tgctcgccca tctcggcgac ggtccctcgc
      661 tcggcgccca ccgcgagcgg ttcggcgcgt tggctctgcc ccggatcgag gcgctcgtcg
      721 cgctcgcgcg tgaggcgcag gtgcgtggcc gaggcggggc tgggttcccg ttcgcggtca
      781 agctcgaagc cgtggccaag gggcgacggc cggtcgtcgt cgtcaacgtc gccgagggag
      841 agccggccag ctggaaggac accgcgctgg ccacactggt gccccaccgg gtgctcgatg
      901 gtgctctggt ggcggcacag gcactcggcg tgcgcgagat acacgtcgtg acgccgagtg
      961 aacgcacggc ggtcggcgcg gccctgcgtc acgccgtcga cgagcggcgc gcggccggcg
     1021 accgcatgcg gttcaccttc cacctcgccg caccggggtt cgtcagcggc caggcgcgcg
     1081 cggtcctcga gctcatggcc ggacgcccga accttcccgt aacggcctgg gagcccgagg
     1141 cgatgtccgg ccaccgcgga cggcccacac tgctgtcgaa cgccgaaacg tacgcccacc
     1201 tcgccatggt ggtgcggctc ggcgccgcgg gctgcgccag gttcggcacc gccgacgagc
     1261 ccgggacgac cctgctcacc atcgacggtg acggaccgtc accactggtc cgcgaggtgg
     1321 cgtatggcgc accgtggagc gccgtgctca gcgaggccga gctcggccag gacgtcatcc
     1381 tgggcggcta ccatggcacg tgggcgccgg ccggtgcgct gcgtgcgctg tcggtcagcc
     1441 gggcccagct cgcggcggcg ggcctggccc tcggcgccgg tgtcgtcctg ccccaggctg
     1501 cggggacctg cccggtcgac cggactgcgg ccatcgtcgg cttcctcgcc agtcgatcgg
     1561 cccggcgttg cggaccgtgc ctgcacgggt tgcccgcgct ggccgctgcg ctcgggacgt
     1621 ggtcgcagct gggcggtttc gctgagcccg tcgctcgggt cgagcagctg ctcactctcg
     1681 tcgagcgccg cggcgcatgt gcccatccgg acggcaccgc ccggctcgtc cgctccctgc
     1741 tggtggcgct gcccgaggag gtcgccgcac accagcgcgg ccggtgcgcg gtcaccgcgg
     1801 gtggggcggc gacggtgcag cgtcggtcgg cgccggtgtc gtcatgacga cggcagtgac
     1861 gcatcggctc cgcgtcgact ggccgctctg cacggcccgc ggactgtgct cggagctgct
     1921 ccccgaggcg gttgctctcg acgagtgggg ctaccccgtc gtgacgtcgg acatcggtga
     1981 cgacctcgtg gccgacgctc gtgccgccgt gaaggcatgc cctcagcgcg cgctgcgtct
     2041 tgtcccccga aactgagtcg gtcgctggcg agtttccctc aggggctagg ggattggacg
     2101 tccgcgggga actgccgccg ggtcgacgat tcacccgcgg gtgcagtgct cggcgtcgtc
     2161 cagcagggcg ataggcggtg tggttaggat ttggcatggc caagaaaccg gcaccctcaa
     2221 agtccgctcc ctccaagaag tc
//
LOCUS       DATAIX010000042         1729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_179898, whole genome shotgun sequence.
ACCESSION   DATAIX010000042 DATAIX010000000
VERSION     DATAIX010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179898"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="VHM65_00740"
     CDS             complement(<1..1018)
                     /locus_tag="VHM65_00740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2704259.1"
                     /translation="MSEAPGRGATGPPPGYPVEWEADVVLRDGSVAHLRPITPADADT
                     LRRFHAAQSDESIYMRFFAPLRELSDRDAYRFTHVDHDLRVALVATIRDDIIGVGRYD
                     KVDESTAEVAFNISDVYQGRGVGSVLLEHLAAIAQDGGVEHFIAEVLPQNRKMLAVFR
                     EAGYEVSQRFEDGVIMVSFDVAATERSTYVRLSREHRAESVSVRSVLHPTSVAVIGAS
                     RREDSIGHRLLSNTLAAGFQGPVYVVNREADEVCGLRAYARIGDVPGPVGLAVIAVPA
                     EGVVDVARQCGLAGVSSLLVVASGFAEAGPEGTALQEALRWEARHGGMRVLGPNSFGV
                     VNQHP"
     gene            1089..1721
                     /locus_tag="VHM65_00745"
     CDS             1089..1721
                     /locus_tag="VHM65_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX2704260.1"
                     /translation="MELRDVIHTRRMVRRYDPDRPVADSVLLDCLDAATRAPSAGFSQ
                     GWDFVVLRLPQERAAFWAATTDPQAPADPWLQGISTAPVLVVACSDPDTYLDRYAEPD
                     KGWTDRATSRWPVPYWDVDAGMASLLILLTAVDHGLGGLFFGVAPDRVAALRHTLGIP
                     ASRRIVGVIALGYAVSHPKSTSLRRGRRGLDEVAHWGRFGVAREPPYPGL"
BASE COUNT          243 a          643 c          595 g          248 t
ORIGIN      
        1 gcgggtgctg gttgaccact ccgaaggagt tcgggccgag gacgcgcatg cccccgtgcc
       61 gggcctccca tcgcagggcc tcctgaagcg ccgtcccttc gggtccggcc tcggcgaagc
      121 cacttgcgac gacgagcaac gagctcactc cggcgagccc gcactggcgg gcgacgtcga
      181 cgacgccctc ggcgggcacg gcgatgaccg caaggccgac cggccccggg acgtccccga
      241 tccgcgcata ggcccgcagg ccgcacacct cgtcggcctc gcggttgacc acgtacaccg
      301 gtccctggaa gcccgccgca agggtgttgg acagcaggcg gtgcccgatc gagtcctcgc
      361 gccggctggc gccgatgacg gcgacggacg tgggatgcag caccgagcgg acgctgaccg
      421 actcggcgcg gtgctcccgc gagagccgga cataggtcga gcgctcggtg gcggccacgt
      481 cgaagctcac catgatgacg ccgtcctcga accgctggct cacctcgtag ccggcctcac
      541 ggaagacggc gagcatcttg cggttctgcg gcaggacctc ggcgatgaag tgctcgactc
      601 ccccgtcctg ggcaatcgcg gccaggtgct cgagcagcac cgagccgaca ccgcggccct
      661 ggtagacgtc ggagatgttg aacgccacct cagcagtgct ctcgtcgacc ttgtcgtagc
      721 gcccgacccc gatgatgtcg tcacggatgg tcgcgacgag ggctacccgc aggtcgtggt
      781 cgacgtgcgt gaagcggtag gcgtcgcggt cgctcagctc acgcagcggg gcgaagaacc
      841 tcatgtagat cgactcgtcc gactgcgcgg cgtggaaccg gcgcagcgtg tcggcgtcgg
      901 cgggggtgat gggccgtaga tgggccaccg acccgtcccg gagcacgacg tcggcctccc
      961 actcgacggg gtaccccggg gggggcccgg tcgcccctcg acccggtgcc tcactcatgg
     1021 ggcccatcgt tccatgcccg ggccttcggc ccgacctggc aagggggtgc gcctgagagg
     1081 atctcaccat ggagctgcgt gacgtgatac acactcgacg gatggtccgc cgatacgacc
     1141 cggaccggcc ggtggccgac tccgtcctgc tcgactgcct cgacgcggcc acccgggccc
     1201 cgagcgccgg cttcagccag ggctgggact tcgtcgtcct tcgcctgccc caggagcggg
     1261 ccgcgttctg ggccgcgacg accgacccgc aggccccggc cgacccgtgg ctccagggca
     1321 tctcgaccgc cccggtcctg gtcgtcgcgt gctcggatcc cgacacctac ctcgaccggt
     1381 acgccgagcc ggacaagggc tggaccgacc gcgccacgag ccgctggccg gtcccctact
     1441 gggacgtcga tgccggtatg gcctcgctcc tgatcctgct caccgccgtc gaccacggcc
     1501 tcggcggcct gttcttcggc gtcgcccccg accgtgtcgc ggcgctccgc cataccctgg
     1561 gcatccccgc cagccgccgg atcgtcggag tcatcgccct cgggtatgcc gtctcgcacc
     1621 cgaagagcac gagcctgcgc cgcggtcggc gcggactgga cgaggtcgcc cactggggac
     1681 gcttcggcgt ggctcgcgag ccgccatacc ccggtttgtg acgatggac
//
LOCUS       DATAIX010000043         1734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_151487, whole genome shotgun sequence.
ACCESSION   DATAIX010000043 DATAIX010000000
VERSION     DATAIX010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151487"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VHM65_00750"
     CDS             complement(<1..376)
                     /locus_tag="VHM65_00750"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704261.1"
                     /translation="MDIWPGKPYPLGATFDGSGVNFALFSEVADEVELALIDNAGAEQ
                     RIPLVETDGFVWHGYVPGLQPGQRYGFRVHGPYDPARGRRCNPAKLLLDPYAKAVDGM
                     VDGDSSLFSYAFDDPKRGLSRKR"
     gene            592..1509
                     /locus_tag="VHM65_00755"
     CDS             592..1509
                     /locus_tag="VHM65_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2704262.1"
                     /translation="MALEIRDVGHAFGERVALDGVSFDVEAGVLTGLLGPNGAGKTTL
                     LRVLLGVLSPDRGDVRYDGRRVVEQDRRRWGYMPQERGLYPGMPAGDQVVHFGRLHGL
                     SRADATVPAHALLDELGLGGRWRERTDKLSGGMQQRLQLATALVHEPEVIVLDEPFSG
                     LDPVAVASLSETLRQRVRSGRTVLFSSHQLDLVQDLCENIVMLDRGRTVLQGTVATLR
                     ASSGRRQLRLHVESPDRDWVRAFPGVSVVSDEADDLRLTVPPGTEPLAILDAARAAGR
                     VVDFGLDLPTLSELFLAVADRTHDQAGVR"
     gene            1539..>1734
                     /locus_tag="VHM65_00760"
     CDS             1539..>1734
                     /locus_tag="VHM65_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704263.1"
                     /translation="MAERALVENVKSRSFKVVAGLLLLMSIAAVTVPQILGEDGTTYT
                     LATVGTAPADVVASQPRRGTP"
BASE COUNT          236 a          618 c          622 g          258 t
ORIGIN      
        1 tgcgctttcg tgacagtcca cgcttggggt cgtcgaaggc gtaggagaac agcgaggagt
       61 cgccgtcgac catcccgtcg acggccttgg cgtaggggtc cagcaggagc ttggccgggt
      121 tgcagcgccg accgcgcgcc gggtcgtagg gtccgtggac tcggaagccg tagcgctgac
      181 cgggctgcag ccctggcaca tagccatgcc agacgaaacc gtcggtctcg acgagcggga
      241 tgcgctgctc ggcgccggcg ttgtcgatca aggcaagctc gacctcgtcg gcgacctcgg
      301 agaacagggc gaagttgacc cctgagccat cgaacgtggc gcccaggggg taaggcttgc
      361 cgggccagat atccatacgt ctctttcggt ggtgcgagtc gcgtcacatc ggtcgtgcgc
      421 agcagagccg cgcgctggac cggggcgaca ctgacacggg ccgcctccat cttgccagcc
      481 agggccgccg cgtggatcga caccccgcac ctcgacgccg gcggacgacc ctgccattgt
      541 ccgcctgccg atccccggga acccgtgtcg acccgttggt agggtcgggg tatggcgctc
      601 gagatccgcg acgtcgggca tgccttcggc gagcgggtag ccctcgacgg cgtgagcttc
      661 gacgtcgagg ctggagtgct caccgggctg ctcggaccca acggtgccgg caagacgacg
      721 ctgctgcggg tcctgctcgg ggtgctgagc cccgaccggg gcgacgtccg gtacgacggg
      781 cggcgcgtgg tcgagcagga ccggcgccgg tggggctaca tgccccagga gcgcgggctc
      841 taccccggga tgccggccgg tgaccaggtc gtccacttcg gtcggctgca cggcctgtcc
      901 cgcgccgacg cgacggtccc ggcccacgcg ctgctcgacg agctgggcct gggcggccgt
      961 tggcgcgagc gcaccgacaa gctctccggc ggcatgcagc agcgtctcca gctggccacg
     1021 gccctcgtgc acgagcccga ggtcatcgtc ctcgacgagc cgttcagcgg cctggacccg
     1081 gtcgccgtcg cgagcctgtc ggagaccctt cggcagcgcg tccggagcgg ccggacggtc
     1141 ctgttctcca gccatcagct cgacctcgtg caggatctct gcgagaacat cgtcatgctc
     1201 gaccggggcc gcaccgtgct ccagggcacg gtcgccaccc tgcgcgcctc gtcgggccgg
     1261 cgtcagctca gactgcacgt ggagtcgccc gaccgcgact gggtgcgcgc cttcccgggc
     1321 gtcagcgtcg tcagtgacga ggccgacgac ctgcggctca ccgtcccgcc gggcaccgag
     1381 ccactcgcca tcctcgatgc cgcccgggca gccgggcgcg tcgtcgactt cgggctcgac
     1441 ctgcccaccc tgtccgagct gttcctggcg gtcgccgacc ggacccacga ccaggcgggg
     1501 gtgcgctgat gggccgatgg tggcgaggga ccgccctggt ggccgagcgc gcgctcgtcg
     1561 agaacgtcaa gtcccggtcc ttcaaggtgg tcgccgggtt gttgctcctc atgtccatcg
     1621 ccgcggtcac ggtgccgcag atcctcggcg aggacggcac gacctacacg ctggccaccg
     1681 tgggcacggc acccgcggac gtcgtggcgt cccagccgcg tcgagggacg ccac
//
LOCUS       DATAIX010000044         1654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_209586, whole genome shotgun sequence.
ACCESSION   DATAIX010000044 DATAIX010000000
VERSION     DATAIX010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209586"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="VHM65_00765"
     CDS             <1..1198
                     /locus_tag="VHM65_00765"
                     /inference="COORDINATES: protein motif:HMM:NF037043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704264.1"
                     /translation="DSGLVREPDRLPAMELDVAIWLVSAEAAPWLERAAGEPDPESLG
                     AADRWRRDLPADRAAAVLTQESLRRAAVRKLGDAARKLFLTRDGLEQATRPTVARWRA
                     TRLATTGVTHVWDLGCGLGLDALALVEAGMGVTAIERDPVTATLAAANLGASARVIVG
                     DVADLYDQVPSDAGVFLDPARRTARGRSWDVADLSPSWDVVTSVLDGGRVACVKLGPG
                     IPYRLLPEHAEAVWVSDQGDLVEASVWAGPGAAPGRRSALLLPQGLSLEAVGGHLPVG
                     PIGSWLYEPDPAVIRAGAIPALGRELGATGIAPGVAYLTTETHQPTPYATAFEVLDHL
                     PYRERDLRAWVKAHGVGTLEIKKRGLEVDPAALRRRLAPKGPASATLVLTPTPDGAVA
                     LVVRRV"
     gene            complement(1195..>1654)
                     /locus_tag="VHM65_00770"
     CDS             complement(1195..>1654)
                     /locus_tag="VHM65_00770"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEX2704265.1"
                     /translation="TRIASQIEDHRDLEHAVALLALLPGIPAVYSGDEQGFTGVKTES
                     FDGDDAVRQQFPAHPYDLLPFGRGLFDLHQRLLGLRRRHRWLWTATATTRELTNEFVV
                     VELTGHDGERLDLALNVAEQPHPLPPGTMLEASDHTATEVPPHGWVVVEP"
BASE COUNT          222 a          593 c          613 g          226 t
ORIGIN      
        1 ggactcagga ctggtccgcg aacccgatag gttgcccgcc atggagctcg acgtcgccat
       61 ctggctggtc tcggccgagg cggcgccctg gctggaacga gccgcggggg agccggaccc
      121 cgagtcgctg ggggccgcgg atcggtggcg gcgagatctc ccggccgacc gtgctgccgc
      181 agttctcacg caggagtcgc tccggcgcgc ggcggtgcgc aagctggggg acgccgcgag
      241 gaagctcttc ctgacccgcg acggactcga gcaggccacc aggcccacgg tcgcgcgatg
      301 gcgcgcaacg aggctcgcga cgaccggggt gacccatgtc tgggacctcg ggtgcgggct
      361 cggactcgac gccctggcgc tggtggaagc ggggatgggc gtcaccgcca tcgagcggga
      421 ccccgtcacc gcgacgctcg ccgccgcgaa cctcggagcg tccgcccgag tgatcgtcgg
      481 cgacgtcgcc gacctgtacg accaggtgcc gagcgatgca ggggtgttcc tcgatcccgc
      541 gcgccgtacg gctcgtggca ggtcatggga cgtggccgac ctcagcccct cgtgggatgt
      601 ggtgaccagc gtcctcgacg gcggccgcgt ggcctgcgtg aagctcggcc ccggcatccc
      661 gtaccggctc ctgcccgagc acgccgaggc cgtctgggtg tcggaccagg gcgacctcgt
      721 cgaggcctcg gtgtgggcgg gtccaggcgc ggcgccggga cgtcgaagcg cgctgctgct
      781 gccgcaaggg ctcagcctcg aggcggtggg tggccacctg ccggtggggc ccatcggaag
      841 ctggctgtac gaacccgacc cagccgtgat tcgtgcgggg gcgatccccg ccctgggccg
      901 cgagctcggg gcgaccggga tcgccccggg cgtggcctac ctcaccaccg agacgcacca
      961 gccgaccccg tacgccaccg ccttcgaggt gctcgaccac ctcccgtacc gcgaacggga
     1021 cctccgggcc tgggtcaagg cccacggcgt cggaaccctt gagatcaaga agcgcggtct
     1081 cgaggtcgac ccggccgcgc tccgccgccg gctggccccg aaaggtcctg cctccgcaac
     1141 cctggtgctg actccgaccc cggacggggc cgtggcgctc gtggtccggc gggtctaggg
     1201 ctcgacgacc acccaaccgt gcggggggac ttccgtagcg gtgtggtccg acgcctccag
     1261 catggtgccg ggcggcagcg ggtgcggctg ctcggccacg ttcagcgcga ggtcgagccg
     1321 ctcgccgtcg tgcccggtca gctcgacgac gacgaactcg ttggtgagct cccgcgtggt
     1381 ggccgtcgcc gtccacagcc accggtggcg ccgacgaagg ccgagcagcc gctggtgcag
     1441 gtcgaacagg ccccttccga acggcagcag atcgtacgga tgggccggga actgctgacg
     1501 tacggcgtcg tcgccgtcga acgactcggt cttcacgccc gtgaagccct gctcgtcgcc
     1561 ggagtagacc gccgggatgc ccggcagcag cgcgagcagc gccaccgcat gctccaggtc
     1621 gcggtgatcc tcgatctgac tcgcgatgcg ggtg
//
LOCUS       DATAIX010000045         2275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_2322, whole genome shotgun sequence.
ACCESSION   DATAIX010000045 DATAIX010000000
VERSION     DATAIX010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2322"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..960)
                     /gene="cysE"
                     /locus_tag="VHM65_00775"
     CDS             complement(280..960)
                     /gene="cysE"
                     /locus_tag="VHM65_00775"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF041874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEX2704266.1"
                     /translation="MSGPDSQPRLTVSPRRSVWPRRSASPGLTVSPVRVGWRERLRED
                     ITTAMAQDPAARSRLETLLTAPGLHAIWAYRLAHRLWHRPGGRLAARILSYVARSGTG
                     VEIHPGATIGRRFFIDHGMGVVIGETAIVGDDVMLYHGVTLGGRTLARGTKRHPTVGN
                     RVTIGTGARVLGPIEIGDGAQIGANSVVVKNVPAGAVATGIPATNRFPAPGEDPYEAL
                     FRHPALFI"
     gene            complement(1010..1936)
                     /gene="cysK"
                     /locus_tag="VHM65_00780"
     CDS             complement(1010..1936)
                     /gene="cysK"
                     /locus_tag="VHM65_00780"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX2704267.1"
                     /translation="MPIYDDVTALVGNTPLVRINRIITSDATVAAKLEFYSPASSVKD
                     RIGVSIVDAAEASGELKPGGTIVEATSGNTGIALAMVGAARGYTVVLTMPSSMSKERR
                     ALLRAFGAELVLTDAALGMKGAVDKADEIAAERGAVLARQFANEANPEIHRRTTAEEI
                     WKDTDGQVDIVVAGIGTGGTITGVGQVLKARKPGVQMIAVEPEESAILTGGAPGPHKI
                     QGIGANFVPEILDRSVYDEVIDINAETAVLWARKAATQEGLLVGISSGAALAAADQVA
                     RRPENAGKTIVVIIPSFGERYLSTVLFEGLVD"
BASE COUNT          321 a          762 c          833 g          359 t
ORIGIN      
        1 ggtcgggcgc gtgtgcgtcc ggcctcacca ttcccctaca tgtggacaag gccgccctga
       61 ttcagccgtg gccacgtgcc gccgccgggt ccctgaggac cccacgggcg ccccaccccg
      121 cgtttccagc ggccccctgg ccagggactg gccgcaggcc attccatcgt tctggacgcg
      181 cgttgtcggg ctcgacatct tggaagaggg gccggggggg cgatctgata caccctgagc
      241 acagatttct ccgctttttc cggcgccggg ccgggtgggt cagatgaaca gcgccggatg
      301 ccggaagagc gcctcgtacg ggtcctcgcc gggggccggg aagcggttcg tggccggtat
      361 gccggtcgcc acagcccctg ctggaacgtt cttgacgacg accgagttgg ccccgatctg
      421 ggcgccgtcc ccgatctcga tggggcccag cacccgggca ccggtgccga tcgtgacccg
      481 gttgccgacc gtgggatgtc gcttggtgcc ccgtgccagg gtgcgaccac caagggtgac
      541 cccgtggtag agcatgacgt cgtccccgac gatcgcggtc tcgccgatga cgacacccat
      601 gccgtggtcg atgaagaaac gccgcccgat cgtcgcgccc gggtggatct cgacgcccgt
      661 gccgctgcgc gcgacatacg acagaatgcg cgcggccagg cggcccccgg gacgatgcca
      721 gagccggtgg gcgagccgat aggcccagat cgcatggagg ccgggcgccg tgaggagggt
      781 ctcgagccgg gatcgggcgg ccgggtcctg cgccatcgcg gtcgtgatgt cctctcgcag
      841 acgctcccgc caccccactc gcaccggcga gacggtgagc ccaggcgacg cgcttcgccg
      901 gggccacacg ctgcgccggg gcgacacggt gagccggggc tgggagtcgg gtcccgacat
      961 acggagcatg ggctgtcctt ggctggaaag gggatgggcg cagagcgggt cagtcgacga
     1021 ggccctcgaa gagcacggtc gagaggtagc gctcgccgaa ggaggggatg atgacgacga
     1081 tcgtcttgcc ggcgttctcg ggacggcggg cgacctggtc ggcggcggcg agcgcggcac
     1141 cggaggagat gccgacgagc agcccttcct gggtcgcggc cttgcgggcc cacagcaccg
     1201 cggtttcggc gttgatgtcg atgacctcgt cgtagacgga ccggtcgagg atctcgggga
     1261 cgaagttggc gccgatgccc tggatcttgt gcggtccggg cgcgccaccg gtgaggatgg
     1321 ccgactcctc cggctcgacc gcgatcatct ggacgccggg cttgcgggcc ttgagcacct
     1381 gtccgacacc ggtgatggtc ccgccggtgc cgatgccggc cacgacgatg tcgacctggc
     1441 cgtcggtgtc cttccagatc tcctcggcgg tcgtgcggcg gtggatctcg gggttggcct
     1501 cgttggcgaa ctggcgggcg aggaccgcgc cgcgctcggc ggcgatctcg tcggccttgt
     1561 cgacggcccc cttcatgccc agcgccgcgt cggtgaggac gagctcggcg ccgaaggccc
     1621 gcagcagcgc gcggcgctcc ttggacatcg aggacggcat ggtgagcacc acggtgtagc
     1681 cgcgcgccgc gccgaccatg gccagcgcga tcccggtgtt gcccgaggtg gcctcgacga
     1741 tcgtgccgcc gggcttgagc tcaccagagg cctcggccgc gtcgacgatc gagacgccga
     1801 tgcggtcctt gaccgagctg gccgggctgt agaactccag cttggccgcc accgtcgcgt
     1861 ccgaggtgat gatccggttg atccgcacga gcggggtgtt gccgaccagt gcggtgacgt
     1921 cgtcgtagat gggcacgggc tcgcctttgc tgccggggac ggagggtctg cggacgacaa
     1981 cccccgtcac cttatgcgta ttccaacgtt gcgttatctt cggaccgatc tcgccggtat
     2041 gccggacacc tcacccctcg ccggtgagcg gggcaggcgg cgcccgcacg gagggttgac
     2101 gctcgtcaca accggggccc gggatgaagc caggagccgt gagcatcaat agactcgcgt
     2161 gcatgagcgc cctccaagtc gtggccctgg tgatctgcgg cgcggtgacg ctgtcaggga
     2221 tcgcgttgct gaccaagacc gtcaacggat tcctcgcgac gttccgcctc gggca
//
LOCUS       DATAIX010000046         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_269301, whole genome shotgun sequence.
ACCESSION   DATAIX010000046 DATAIX010000000
VERSION     DATAIX010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269301"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1873)
                     /locus_tag="VHM65_00785"
     CDS             complement(128..1873)
                     /locus_tag="VHM65_00785"
                     /inference="COORDINATES: protein motif:HMM:NF039189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HEX2704268.1"
                     /translation="MAALRVPGEGIDTVIAGLFSLVLLALVAAAVVGVVNAVRQPHPG
                     HSGSTPAAAARRFVVFALLFVLVVIAAIGVSGLLGRLLDLGNDLAGADVSGLALSLTF
                     ALIGGASAALLWWVLWRHLDTEEERSSIPWSLYVAGVATVALITATMSLMSALAQLIG
                     GRWEPQALASAIIWAGVWVWHRAMAKNRVKSPTRLTDLAPVAGSTYGLLVGAGAAVAT
                     LQTLFDAALETFGDRATIGDPWWVLALQSLIWAVGGAAVWWWHWVRDESRRLDTGAAD
                     AALVLVGIVAAAVLALGGAGTVLYTVLRLGFDRVDPASQILEPLPLALSALAVGALVW
                     TFHRAVIAPRGDRVREASRLLVSGVGLVATATGIGIIVNSILDASTATLAGAGGRGLL
                     LGGLSALVVGAPVWWLTWQPLVKITEPERVRATGRRVYLVAVFGASAVVALVTLLVIG
                     YRVFELALGDLTGEGLVERVRAPFGLLVATGLVAGYHYSVWRGDRSLLAAHPVAKTVG
                     SVILVTGSDPGPLTNAISDVTGAAVTVWLRAGTDLDGPSEDQVAQALAGVSGRCVMVL
                     TGAGGRIEVIPLQPA"
     gene            complement(1831..>2096)
                     /locus_tag="VHM65_00790"
     CDS             complement(1831..>2096)
                     /locus_tag="VHM65_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704269.1"
                     /translation="LTHAPDRCDPMIPRPSGTLRVRLLTTDERADSARVRVLVVQSSG
                     GGPLGSSDYDTEDAFDLVKVAGQWKVDTAPWQLSVCPEKGSTP"
BASE COUNT          350 a          718 c          796 g          232 t
ORIGIN      
        1 ttcaaggaga actcggcgat gctcttcggc gacgccaagg tccgcgtcga ggacatcatc
       61 agggccctct gaccgggggc tgagccctat gaccggggct cagaccgggg gctcagccca
      121 gcagggctca ggccggctgc agcgggatga cctcgatgcg gccgcccgca ccggtgagga
      181 ccatgacgca cctgcccgag acgccggcga gcgcctgggc gacctggtcc tcgctcggcc
      241 cgtcgaggtc ggtgccggcc cgcagccaga cggtgacggc cgcccccgtc acgtcgctga
      301 tggcgttcgt gaggggtccg gggtccgacc cggtgaccaa gatgacggag ccgacggtct
      361 tggccacggg gtgggcggcc agcagcgacc ggtcacccct ccacaccgag tagtggtagc
      421 cggcgaccag gccggtggcc acgagcagcc cgaacggcgc ccggacccgc tcgacgagac
      481 cctcgccggt gaggtcgccc agggcgagct cgaagacccg gtagccgatg acgaggaggg
      541 tgaccagagc gacgacggca ctcgcgccga agaccgcgac gaggtagacg cgccggcccg
      601 tggcccggac gcgctctggc tcggtgatct tgaccagggg ttgccaggtg agccaccaca
      661 ccggcgcgcc gacgacgagc gcgctcagac cgccgaggag cagcccccgc ccgccggcgc
      721 cggcgagcgt cgcggtgctc gcgtcgagga tcgagttgac gatgatgccg atccccgtcg
      781 cggtcgcgac gaggccgacc ccggagacga ggagccggga cgcctcacgg acccgatcgc
      841 cgcgaggtgc gatgacagcg cggtgaaagg tccacaccag ggcgcccacg gccagcgccg
      901 agagggcgag cggcagcggt tcgaggatct ggctcgccgg gtcgacccgg tcgaacccga
      961 gccggagcac ggtgtagagc accgtgccgg ccccgcccag tgccaggacg gccgcagcga
     1021 cgatgccgac gaggaccagg gccgcgtcgg ctgcaccggt gtcgaggcga cggctctcgt
     1081 cgcgaaccca gtgccaccac cacaccgcgg ctccgccgac agcccagatg agcgactgga
     1141 gcgcgaggac ccaccagggg tcgccgatcg tcgcccggtc gccgaaggtc tccagcgccg
     1201 cgtcgaacag agtctgcagc gtggccacgg ccgcaccggc gccgacgagg aggccgtagg
     1261 tggaaccggc gaccggtgcc aggtcggtga gccgggtggg gctcttgacc ctgttcttgg
     1321 ccatcgcgcg atgccagacc cacaccccgg cccagatgat ggcgctggcg agagcctgcg
     1381 gctcccagcg ccctccgatg agctgggcga gcgcggacat gagcgacatc gtggcggtga
     1441 tgagcgcaac cgtggcgacg ccggccacgt agagggacca ggggatggac gagcgttctt
     1501 cctcggtgtc gaggtgacgc cacaggaccc accagagtag ggcggccgag gccccgccga
     1561 taagtgcgaa cgtcagcgac agcgcgaggc cgctgacgtc ggcgccggcg aggtcgttgc
     1621 ccaggtccag gaggcgtccc agcagcccgc tcaccccgat cgcggcgata acgacgagga
     1681 cgaacagcag ggcgaagacc acgaaacgcc gggctgcggc tgcgggggtg gacccactgt
     1741 ggccggggtg tggctggcgc acggcattga cgacgccgac aaccgcggca gcgacaagag
     1801 cgaggaggac gaggctgaac aggccggcga tcacggtgtc gatcccttct ccgggcacac
     1861 ggagagctgc cacggggcgg tgtcgacctt ccactgcccg gcgaccttga ccaggtcgaa
     1921 ggcgtcctcg gtgtcgtagt cggaggagcc gagcgggccg ccgccggagc tctgcacgac
     1981 caggacccgg acgcgggccg agtcggcgcg ctcgtcggtc gtgaggaggc gcacgcgcaa
     2041 cgtgccgctc gggcgaggga tcatcgggtc gcagcggtca ggggcgtggg tgaggt
//
LOCUS       DATAIX010000047         2160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_239499, whole genome shotgun sequence.
ACCESSION   DATAIX010000047 DATAIX010000000
VERSION     DATAIX010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239499"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..277
                     /locus_tag="VHM65_00795"
     tRNA            205..277
                     /locus_tag="VHM65_00795"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:238..240,aa:Val,seq:tac)
     gene            384..1226
                     /locus_tag="VHM65_00800"
     CDS             384..1226
                     /locus_tag="VHM65_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX2704270.1"
                     /translation="MLEALASLYPPGSADDWDRVGLVTGDPADLVRRIHFAVDPTLAV
                     IGEAVAAGADLLVTHHPLLLRGVHALPMTTAKGASVTALIRGKVALYVAHTNADKADP
                     GVCDALAAACGLTEAQPLFTEHGFGVGRVGTLETGGGMTLRGFAARLTEALPKTVGGV
                     RVCGPPDARVERVAVVGGAGDGFFDAVRASGADVYVTADLRHHPALEAREEARGGTPY
                     LIDAGHWATESLWLPSARRELFRVLAERRGAATTVETYISGLRTEPWTFVLGANAAAD
                     AEED"
     gene            1351..1977
                     /locus_tag="VHM65_00805"
     CDS             1351..1977
                     /locus_tag="VHM65_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEX2704271.1"
                     /translation="MAATDLDRRLVQARTARDDIARDVTKADQDVQLVRDRAARDQAR
                     LDSGFGTAKDLQALQHELASLARRQGDLEDIELEVMERAEGLEHAVTALDAERAELAE
                     RIEAVRAERDAALSTLDDEAAGLRARRTDILPRVGEDLVTLYEKIRASSGGIGAAELT
                     SRRCGGCRLELNSVDMNRIVAAPADEVLRCEECRRILVRTAESGIYSG"
     gene            1974..>2160
                     /locus_tag="VHM65_00810"
     CDS             1974..>2160
                     /locus_tag="VHM65_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704272.1"
                     /translation="MSGPPGMPRRRLLVEADGGSRGNPGIGGYGALVRDRASGEVIAE
                     RAEPLGQVSNNVAEYRGL"
BASE COUNT          293 a          809 c          795 g          263 t
ORIGIN      
        1 gctccgaagc cgccccgtgc gactgcccat tgtgtcaggc gccgggcccg ggtcacggcg
       61 gcacggttgc agtcgtagcg tcgtgacctg ccctgtgcgc cgcgacttct gcgggctcgg
      121 cgaagcccgg gccgccagcg cctgacacaa tgggcagtcg cacggggcgg cttcggagct
      181 gagccgaggc ccgccagaag cacggggcct gtagctcagt tggcagagca ccgcgtttac
      241 accgcgggtg tcgtcggttc gagcccggcc gggcccacat gagccagacc gccagcgacc
      301 cctctgcgcc gacggaagcg ccgacggaag cgccgacgga tgcgccgacg gatgcgccga
      361 ccggcgctcc gacgctcggc gaggtgctcg aggcgctcgc gagtctctat ccacccggct
      421 cggccgacga ctgggaccgg gtcgggctcg tcaccggaga ccccgcggac ctggtcaggc
      481 gcatccactt cgcggtcgac cccacgctcg ccgtcatcgg cgaggccgtg gcggcggggg
      541 cggacctgct cgtcacgcac catccgctgc tcctgcgcgg ggtccacgcc ctcccgatga
      601 cgaccgccaa gggcgcgtcg gtcaccgctc tgatccgcgg caaggtcgcc ctctacgtcg
      661 cgcacaccaa cgccgacaag gccgaccccg gggtctgcga cgccctggct gcggcctgcg
      721 ggctcaccga ggcccagccg ctcttcaccg agcacgggtt cggcgtcgga cgcgtcggca
      781 cgctcgagac aggcgggggt atgacgctgc gcgggttcgc cgcccggctc accgaggccc
      841 tgcccaagac cgttggggga gtgcgggtct gcggtccgcc agacgcccgg gtcgagcggg
      901 tcgccgtcgt cggtggcgcg ggtgacgggt tcttcgacgc ggtgcgggcc agcggcgccg
      961 acgtctacgt gaccgctgac ctgcggcacc atccggcgct cgaggcgagg gaagaggctc
     1021 gcgggggcac gccatacctc atcgacgccg gtcactgggc gaccgagtcg ctctggctgc
     1081 ccagcgcccg gcgcgagctg ttccgagtcc tcgccgaacg gcggggtgcg gccactacgg
     1141 tggagaccta catctccggc ctgcgcaccg agccgtggac cttcgtcctc ggcgccaacg
     1201 cggcggccga cgccgaggag gactgacctg aacgccgacc cgacccgcca gctgtccctg
     1261 ctcgacctgc aaggggtcga cacccggctc gaccagatcg cccacgcagc gcgcacgtcg
     1321 ccgctgcacg cactgctcgc gacggtcgag gtggcggcga ccgacctgga ccggcggctg
     1381 gtgcaggccc ggacggcgcg cgacgacatc gcccgcgacg tcaccaaggc cgaccaggac
     1441 gtccagctcg tccgggaccg ggcggcccgc gaccaggccc ggctcgactc ggggttcggg
     1501 acggccaagg acctgcaggc gctgcagcac gagctcgcct cgctcgcccg gcgccagggc
     1561 gacctcgagg acatcgagct cgaggtcatg gagcgggccg aagggcttga gcacgcggtc
     1621 accgcgctcg acgccgagcg cgccgagctg gccgagcgga tcgaggcggt gcgcgccgag
     1681 cgggatgccg cgctgagcac cctcgacgac gaggcggcgg gcctgcgggc caggcgcacc
     1741 gacatcctgc cccgcgtcgg cgaggacctc gtcacgctct acgagaagat ccgcgcgtcc
     1801 tccggcggga tcggcgccgc cgagctcacg tcccgccggt gcggcggctg ccggctcgag
     1861 ctcaactcgg tcgacatgaa ccgcatcgtc gccgcgcccg ccgacgaggt cctgcgctgc
     1921 gaggagtgcc gccggatcct cgtgcgtacc gccgagtccg ggatctactc cgggtgagcg
     1981 ggccgcccgg tatgccgcgc cgccgcctcc tcgtcgaagc cgacggcggg tctcggggca
     2041 accccggcat cggcgggtac ggcgcgctgg tgcgcgaccg ggcgagcggt gaggtcatcg
     2101 ccgagcgggc cgagcccctc gggcaggtga gcaacaacgt ggccgagtac cgcgggctca
//
LOCUS       DATAIX010000048         3581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_180011, whole genome shotgun sequence.
ACCESSION   DATAIX010000048 DATAIX010000000
VERSION     DATAIX010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180011"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VHM65_00815"
     CDS             <1..915
                     /locus_tag="VHM65_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704273.1"
                     /translation="TPASPEAPARPLTRAAALNLGLTPAQLRGPGFVRLFTNVYAPAG
                     IAHTIGARAAGALLIAPPGSLAARHTAAALWGAPVPSWPTIELCIPRPHQMSSAGVRV
                     SRPATLPQAATFQGIPVTTPGRTFLDCAESFGLVDLVVLGDYLVRNNHVAESELGKAT
                     AAWHRRGAELARQAAGLVRAGVDSPMETRLRLLLVLAGFPEPVVNHILRDDAGDWLYR
                     LDLSYPQLRIAIEYDGRHHGESSAQWVKDVGRREALDNAGWRLMVVLSSDIYQHPARL
                     LARAAQLLLDRGMPVTVRQDWRRYFPVR"
     gene            1144..2133
                     /locus_tag="VHM65_00820"
     CDS             1144..2133
                     /locus_tag="VHM65_00820"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF003916.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX2704274.1"
                     /translation="MSTAPVKIAVTGAAGQIGYSLLFRIACGDLLGPDTPVQLRLLEI
                     TPALKALEGVVMELEDCAFPTLAGVEIGDEAATIFDGANLALLVGARPRTAGMERADL
                     LSANGAIFTAQGKAINDHAASDIRIGVTGNPANTNALIAMTNAPDIPRERFTALTRLD
                     HNRALSQLARKAGCRTADIRKLTIWGNHSATQYPDIFHAEINGQNAAALVDDQAWLED
                     DFIPTVAKRGATIIAARGSSSAASAASATIDAARDWLLGSAEGDWVSMAVASDGSYGV
                     PEGIVYSYPVTTENGEWSIVQGLQIDDFSRARMDATAAELVDERAAVTELGLI"
     gene            complement(2186..2461)
                     /locus_tag="VHM65_00825"
     CDS             complement(2186..2461)
                     /locus_tag="VHM65_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3017 domain-containing protein"
                     /protein_id="HEX2704275.1"
                     /translation="MKLRPLADLGAWWAPAVIAVGGLAVAVAGWVRVGGWAVAASLVL
                     SAVLRVVGRRHPSGLEVRSVTADVLALLVLACLLAYAVAIVDLSPPL"
     gene            complement(2458..3327)
                     /locus_tag="VHM65_00830"
     CDS             complement(2458..3327)
                     /locus_tag="VHM65_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019261778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEX2704276.1"
                     /translation="MSAILLDGLTTLAAIKLELRARVAALAERGAVPGLGTVLVGDDP
                     GSHWYVGAKHKDCAEIGIRAIRHDLPATASQAEVEAVVDELNADPGCSGLIVQQPSGL
                     DEFAILSRVDPGKDVDGLHPYNLGCLVLGHPAPLPCTPMGAVELLRRYDVPLAGAKVT
                     VVGRGLTVGRPLGLILTRRSENSTVTLCHTGTRDLAAAVRDADIVVAAAGVHHIITAD
                     MVKPGAAVLDVGVSRVVEDGRSVVGGDVHPDVAAVAGWLSPNPGGVGPMTRALLLTNV
                     VAAAERLAGSAPR"
     gene            3382..>3581
                     /locus_tag="VHM65_00835"
     CDS             3382..>3581
                     /locus_tag="VHM65_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704277.1"
                     /translation="MRAAPVTTAGTAACDNGGDRSLVFTTPFPSAWGASATDASSGRS
                     VWQYGEPVAAHAARRPSHDLTGA"
BASE COUNT          544 a         1353 c         1180 g          504 t
ORIGIN      
        1 accccagcct cacctgaagc accggcccgg ccactcactc gagccgcggc gctcaacctc
       61 gggctcaccc cggcccagct gcgtggtccc ggcttcgtcc ggctgttcac caacgtctat
      121 gcgccggccg gcatcgcgca caccattggc gcccgtgcgg ccggcgctct gctcatcgcc
      181 cccccggggt cgctggctgc ccgacatacg gcagcggctt tgtggggggc gccggttccg
      241 agctggccga ccatcgagct gtgcatcccg cgtccccacc agatgagctc ggccggcgtg
      301 cgggtcagcc gtccggcgac gctccctcag gccgccacct tccagggcat cccggtgacc
      361 acacccggcc ggacatttct cgactgcgcc gagtcctttg ggctggtgga cctggtggta
      421 ctcggcgact acctcgtgag gaacaaccac gtcgcggagt ccgagctggg caaggccacc
      481 gcggcatggc accggcgcgg cgcggagctg gccaggcaag ccgcagggct ggtccgggcg
      541 ggagtcgact cgcccatgga gacgcggctg cgtctgcttc tcgtccttgc cggctttcct
      601 gaacccgtcg tcaaccacat cctgcgggac gacgccggcg actggctcta ccgcctcgac
      661 ctgtcctatc cgcagctgcg gatcgccatt gagtatgacg ggcgccacca cggcgagtcc
      721 tcggcgcagt gggtcaagga tgtcggtagg cgtgaagccc tggacaacgc gggatggcgg
      781 ctgatggtcg tgctgtccag cgatatctac cagcatcctg cccggctcct ggcgcgggcc
      841 gcgcagctgc ttctcgatcg cggtatgccg gttacggtaa ggcaggactg gcgccggtac
      901 ttccccgtcc gttgaggcca aggccctcgg ccggccgacg tcacctcacc catggtgacc
      961 tccttgtgat cgacccccac tgcatccacg accacgtcac ccgaggtgac ctcgcctgcg
     1021 ctcgaccccc gaccacgccg acctgcggtg aggcgcacgt cataccggct ctggatctga
     1081 ccccgcaacg ggcctgctga tagcgtcgta ccacccattt cacaccgtcg caaagggccc
     1141 actgtgagca ctgcacccgt caagatcgcc gtcaccggcg cggccggcca gatcggctac
     1201 agcctcctgt tccggatcgc ctgcggggac ctgctcggcc cggatacccc ggtgcagctg
     1261 cgcctgctgg agatcacgcc cgccctcaaa gccctcgaag gcgtcgtcat ggagctcgag
     1321 gactgcgcct tcccgacact ggccggtgtc gagatcggcg acgaggcggc gacgatcttc
     1381 gacggggcga acctcgccct gctcgtcggc gcccgccctc gcaccgcggg gatggagcgc
     1441 gccgacctgc tctcggccaa cggtgcgatc ttcaccgccc aaggcaaggc catcaacgac
     1501 cacgcggcga gcgacatccg catcggcgtc accggcaacc cggccaacac caacgccctc
     1561 atcgcgatga ccaacgcgcc ggatatcccg cgcgagcgct tcaccgcgct gacccgcctg
     1621 gaccacaacc gggccctgtc ccagctcgcc cgcaaggcgg gctgccgcac tgccgacatc
     1681 cgaaaactga ccatctgggg caaccactcg gcgacgcagt accccgacat cttccacgcc
     1741 gagatcaacg gccagaacgc cgccgccctc gtcgacgacc aggcctggct cgaggacgac
     1801 ttcatcccga ccgtggccaa gcggggcgcg acgatcatcg ccgctcgcgg atcgtcctcg
     1861 gccgcgtccg ccgcctcggc cactatcgat gcggcccgcg actggctgct cggcagcgcc
     1921 gagggcgact gggtgtcgat ggcggtggct tccgacgggt cctacggggt gcccgagggc
     1981 atcgtctact cctaccccgt gacgaccgag aacggcgagt ggtcgatcgt ccagggtctg
     2041 cagatcgacg acttctcccg ggccaggatg gacgccactg cggccgagct cgtcgacgag
     2101 cgcgcggccg tgaccgagct cggcctcatc tagggacgct cagcctcgcc gccgcggggc
     2161 tcccgctcac agcgggggct agagctcaca gcgggggcga gaggtcgacg attgcgacgg
     2221 catacgcgag caggcaggcc agcaccagca gcgcgaggac gtcggcggtc accgagcgga
     2281 cctcaagtcc gctgggatgc ctgcgcccca ctacgcgcag caccgccgac agtacgaggc
     2341 tcgcggccac cgcccatccg ccgacccgca cccacccggc cacggccacg gccagacccc
     2401 cgacggcgat caccgcggga gcccaccagg cgccgaggtc ggcaagcgga cgcagcttca
     2461 cctcggcgca gatcccgcga gacgctcggc tgcggccacg acgttggtga gcagcagcgc
     2521 ccgcgtcatg ggaccgacac caccagggtt ggggctgagc cagccggcca ctgcggccac
     2581 gtcagggtga acgtcgcccc cgacgacgct gcggccgtcc tcgacgaccc ggctcacccc
     2641 gacatcgagc accgccgctc ccggcttgac catgtccgcc gtgatgatgt ggtgcacacc
     2701 ggccgcggcc accacgatgt ccgcgtcccg cacggctgcg gccaggtcac gggtccccgt
     2761 atggcacagg gtgaccgtcg agttctccga gcggcgggtg aggatgagac ccagcgggcg
     2821 cccgacggtg agtccccgac ccacgacggt gaccttggca cccgcgagcg ggacgtcata
     2881 ccggcgcagg agctcgactg cccccatcgg cgtgcagggc aggggggcgg ggtgcccgag
     2941 gacgaggcag cccaggttgt aggggtgcag gccgtcgaca tccttgcccg ggtcgacgcg
     3001 cgacaggatc gcgaactcgt ccagcccgct gggctgctgg acgatgagcc cgctgcagcc
     3061 ggggtcggcg ttgagctcgt cgacaacggc ctcgacctcg gcttggctcg cggtcgcagg
     3121 caggtcgtgg cggatcgcgc ggatgccgat ctcggcacag tccttgtgct tggccccgac
     3181 gtaccagtgg ctgcccgggt catccccgac gaggaccgtg ccaaggccgg gcacggctcc
     3241 ccgctcggcc agggctgcca ctcgcgcccg cagctcgagc ttgatcgcgg cgagggtggt
     3301 caagccgtcg aggagtatgg cgctcacgtc tggtcatcct gccacgccgg tacggtgccc
     3361 gtgcgcgccg gtacggtgcc cgtgcgcgcc gcaccagtga caacggctgg gaccgctgcc
     3421 tgtgacaacg gcggcgaccg cagcctggtc ttcacgacac ccttcccgag cgcctggggc
     3481 gcatccgcca ccgacgcgag ctctgggcgt agcgtctggc agtatggcga acccgtcgca
     3541 gcgcacgctg ctcgtcgtcc atcacacgac ctcaccggcg c
//
LOCUS       DATAIX010000049         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_32636, whole genome shotgun sequence.
ACCESSION   DATAIX010000049 DATAIX010000000
VERSION     DATAIX010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32636"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VHM65_00840"
     CDS             <1..381
                     /locus_tag="VHM65_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2704278.1"
                     /translation="TVLTLAGQPPAMADFARIWQAADKIVYSTTLERVSSARTRIERH
                     FDPEAIRALKESAGRDISVGGPDLAAQAIRAGLVDECHLFVAPIVVGGGTASLPGHVR
                     LTLELLDVRRFGNGMVHLHYRTRT"
     gene            514..807
                     /locus_tag="VHM65_00845"
     CDS             514..807
                     /locus_tag="VHM65_00845"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX2704279.1"
                     /translation="MESIHKQFREQGLVRPTSGRILGGVCAGVARRFNIDPWAARALF
                     FVTLVLIPGSQILIYPILWVFMPDEAAPVAPYTPPQTQRYEPQDTPPPPPPAA"
     gene            complement(813..998)
                     /locus_tag="VHM65_00850"
     CDS             complement(813..998)
                     /locus_tag="VHM65_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003949541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HEX2704280.1"
                     /translation="MADSSRDSDRLLTPAEVAGMFRVDPKTVTRWAKAGKLGAIRTLG
                     GHRRYRESEVLGLLDKS"
     gene            1174..>2062
                     /locus_tag="VHM65_00855"
     CDS             1174..>2062
                     /locus_tag="VHM65_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX2704281.1"
                     /translation="MTPGTSTPSPTPRPDPTADLLGAAEHELVAVCHDRDTGLRAIIA
                     LHSTALGPGLGGTRFYPYPSFAAALTDVLALSKAMSYKNALAGLDHGGGKAVIIGNPV
                     TEKAPELLRAYGRFVESLGGRYVTACDVGTYVADMDVIGEATRFATGRSVARGGAGDS
                     SVLTAYGVFEGMRAAAQQAWGTPTLAGRRVGIAGVGKVGRLLAAHLLDDGADVVVTDV
                     SPAATQSLTQRFPQVEVAPDVATLCAMAIDVYSPCALGGALDPATVESLQARIVCGGA
                     NNQLAVDGPDGTADRLQERG"
BASE COUNT          305 a          773 c          697 g          287 t
ORIGIN      
        1 accgtgctca ccctcgccgg ccagccaccg gccatggcgg acttcgcgcg gatatggcag
       61 gcggcagaca agatcgtcta ctccacgacc ctggagaggg tgtccagcgc caggacccgg
      121 atcgagcgac acttcgaccc tgaggcgatc cgggcgctga aggagtcagc ggggcgtgac
      181 atcagcgtgg gcggtcccga cctggccgcc caggcgatca gggccggttt ggtcgacgag
      241 tgtcacctct tcgtcgcgcc gatcgtcgtg ggaggcggca cggcgtccct ccccggccat
      301 gtccgcctca cgctcgagct gctggacgtg cgccggttcg gaaacggcat ggtccacctg
      361 cactaccgca cgaggacgtg aggagggcga cgaggcgggt cagggtcggc tcaggtgccg
      421 gtcagggtcg accctcatag cggccgcgac gatccaggag gaggcttgtc tcaacgcccc
      481 aaccagggga tccggacacc aaggagcacc accatggaaa gcatccacaa gcagttccgc
      541 gagcagggcc tggtccggcc cacctccggc cgaatcctcg ggggtgtctg cgccggcgtg
      601 gctcgccggt tcaacatcga cccgtgggcg gctcgcgcgc tgttcttcgt cacgctcgtg
      661 ctgatccccg gcagccagat cctgatctac ccgatcctgt gggtcttcat gcccgacgag
      721 gctgcgccgg tcgcgccata cacgccgccg cagacccagc gctacgagcc gcaggacacg
      781 cccccgccgc ccccgccggc tgcgtgagcc ggtcagctct tgtcgaggag tccgaggacc
      841 tcggactcgc ggtagcgccg atgcccgccg agagtgcgga tcgccccgag cttgccggcc
      901 ttggcccagc gagtcacggt tttcgggtcg acccggaaca tgcccgcgac ctcggcgggc
      961 gtcagcagcc ggtccgagtc gcgtgatgag tccgccatga tgtgctactt cctctggtcg
     1021 agcgccaccc caccccggcg cgcccctgga ggcccagtat tgcccgattc gcgcgggcgc
     1081 gggacgaatc gggcagaact cgtggcccgc cgagtggcgt agccttcgga ccggaccaga
     1141 cccgccagca tcggcacaca caggaccgag accgtgaccc caggcaccag caccccctcg
     1201 cctactccca ggcccgaccc cacggctgac ctgctcgggg ccgccgagca cgagctggtc
     1261 gccgtgtgcc acgaccggga caccggcctg cgggcgatca tcgccctgca cagcaccgcc
     1321 ctgggcccgg gcctcggcgg cacccgcttc tacccctacc cgtcgttcgc ggccgccctg
     1381 accgacgtgc tcgccctgtc caaggccatg tcctacaaga acgccctggc cggtctcgac
     1441 cacggcggcg gcaaggccgt catcatcggc aacccggtga cggagaaggc ccccgagctg
     1501 ctgcgcgcct acggccggtt cgtcgagagc ctcggcggcc gctacgtcac cgcctgcgac
     1561 gtcggcacgt atgtcgcgga catggacgtc atcggcgagg ccaccaggtt cgcaacgggc
     1621 cgttccgttg cgcggggagg cgcgggcgac tcgagcgtcc tcacggccta cggggtcttc
     1681 gagggcatgc gtgcggccgc ccagcaggcg tgggggacac ccaccctcgc cggtcgccgg
     1741 gtcgggattg ccggcgtggg caaggtcggc cgcctgctgg ccgcgcacct gctcgacgac
     1801 ggagccgatg tcgtcgtcac cgacgtcagc cccgccgcga cgcagtcgct gacccagcgg
     1861 ttcccccagg tcgaggtggc gccggacgtg gcgacgttgt gcgccatggc tatcgacgtg
     1921 tactcaccgt gcgccctcgg cggcgctctc gaccccgcca cggtggagtc cctccaagcc
     1981 cggatcgtct gcggcggcgc caacaaccag ctcgccgtcg acgggccgga cggcaccgcc
     2041 gaccggctcc aggagcgcgg aa
//
LOCUS       DATAIX010000050         1514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_92170, whole genome shotgun sequence.
ACCESSION   DATAIX010000050 DATAIX010000000
VERSION     DATAIX010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92170"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1095
                     /locus_tag="VHM65_00860"
     CDS             184..1095
                     /locus_tag="VHM65_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEX2704282.1"
                     /translation="MNPDLDTLATRLYVTIDDLLVEHPEWAPERPEVGIAPRLTDAEL
                     LTLAVLQALLGFTSEARFIRHAHAHLRPWFPYLPARPGYNKRLRHAGELMRHVIAHLA
                     RTCPSFTDDLWLVDSTPVECGRSRETVKRSDLAGWATYGYCSSHSRFFWGLRLHLIAT
                     PSGLPVAFALTGAKTDERDTALDMIALDPDLWRPGQILMADKGYRRASFEAELADAGI
                     TLIRPTLGAEKPRPHQRFLRPFRQIIESVNQTLKAQLDLERHGGRTKPGVCARVLQRL
                     LALTAAIWHNETTDRPGPARSLTAYDH"
     gene            complement(1115..>1514)
                     /locus_tag="VHM65_00865"
     CDS             complement(1115..>1514)
                     /locus_tag="VHM65_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704283.1"
                     /translation="LTILQIVWAPGMRLFPHDHDMWACIGIYAGSEDNEFFRRSPGGR
                     GGLRPTGGKRLEPGTATLLGDDVIHAVANPSTVAATAAIHVYGGDFMARPRSQWLEPA
                     LLEEPYDMAAVTARFDEANERWARARGGTP"
BASE COUNT          259 a          587 c          444 g          224 t
ORIGIN      
        1 tgctcgagag gtgcctggac gtgagccgat tcctgctcga gtcggccccg gcctggatcg
       61 acgagcccta gatgagtagt tccaagggat tcgcgcgctc cgcgcgatca tccaccagat
      121 aggtagcgag ggtgtccacg atcagggggc tacaacaaac cctgatcccg aaggacacag
      181 cgtatgaacc ctgacctgga caccctcgcg acccgactgt acgtcaccat cgacgatctc
      241 ctcgtcgaac acccagagtg ggcacccgag cgacccgagg tcgggatcgc gccgaggctc
      301 accgacgccg agctcctcac gctcgccgtg ttacaggccc tgttggggtt cacctccgaa
      361 gcgcgcttca tccgccacgc gcacgcccat ctgcgcccct ggttcccgta cctgcccgca
      421 cgccccggct acaacaagcg gctccgccac gccggtgaac tcatgcgaca cgtcatcgcc
      481 cacctggccc ggacatgccc gtcgttcacc gacgacttgt ggctcgtcga ctccaccccc
      541 gtcgagtgcg gccgcagccg cgagaccgtc aagcgatccg atctcgccgg ctgggccacc
      601 tacgggtact gctcgtcaca ctcccggttc ttctggggac tccgcctgca cctcatcgcc
      661 acaccatcag ggctcccggt cgcgttcgcg ctcaccggag cgaagaccga tgaacgcgac
      721 accgccctcg acatgatcgc cctcgacccc gacctatggc gccccggcca gatcctcatg
      781 gccgacaagg gctaccgacg cgccagcttc gaagccgaac tggccgacgc cggtatcacc
      841 ctcatccgcc ccaccctggg cgccgagaag ccccgacctc accagcggtt cttgcgcccg
      901 ttccgccaga tcatcgagtc ggtcaaccag accctcaagg cccaactcga tctcgaacgc
      961 cacggcggac gcaccaaacc cggcgtctgc gcccgagtcc tgcaacggct cctcgcgctc
     1021 accgccgcga tctggcacaa cgaaaccacc gaccggcccg gcccagcccg cagcctcacc
     1081 gcctacgacc actgaacccc ttggaaccaa tcatctaggg ggtacctccg cgggcgcggg
     1141 cccaccgctc gttcgcctcg tcgaaccggg cggtgaccgc agccatgtca taaggctcct
     1201 cgagcagggc cggctcgagc cactggctgc ggggccgggc catgaagtcg cctccgtaga
     1261 cgtggatggc cgccgtggcc gccactgtgg agggattggc cacggcgtgg atcacgtcgt
     1321 cgccgagcag ggtggcggtg ccgggctcga gccgcttgcc gccggtgggc cgcagtccgc
     1381 cgcgcccacc cgggctgcgc cggaagaact cgttgtcctc cgagccggcg tagatgccga
     1441 tgcacgccca catgtcgtga tcgtggggga acagccgcat tcccggcgcc cacacgatct
     1501 gcaggatcgt cagg
//
LOCUS       DATAIX010000051         1595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_92214, whole genome shotgun sequence.
ACCESSION   DATAIX010000051 DATAIX010000000
VERSION     DATAIX010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92214"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VHM65_00870"
     CDS             complement(<1..346)
                     /locus_tag="VHM65_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704284.1"
                     /translation="MELTAYRDVLAVREARSAMLLGFVIRIPMFAASVILTIHIVSTL
                     RQTYGAAGVATMVVTVALAVSGPWRGRLLDRIGLRRTMTPSIVAQAVCWSIAPFVSYG
                     PLLVLAGLGALFA"
     gene            411..800
                     /locus_tag="VHM65_00875"
     CDS             411..800
                     /locus_tag="VHM65_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704285.1"
                     /translation="MPCPPPAGTAYRLRHGSGRRRGSSLNLFRFRTQIRNPATILYNT
                     GPITDITSASWNRPQFYSVTRIARGHPPVLAKGLGALRPFTMAHLIPLAATAGVNGVW
                     STASRRTATIRVEGKGKKVAARLDWRG"
     gene            797..1498
                     /locus_tag="VHM65_00880"
     CDS             797..1498
                     /locus_tag="VHM65_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2704286.1"
                     /translation="MSTQGMVLVVEDDHAIATMLTLALRKAGYDAQVASDGDAALAAV
                     AAIRPDAVLLDVGLPGRDGIDVCRQLRARGDWTPVLFVTARDDEIDRIVGLELGADDY
                     ITKPFSPREVVARIGTVLRRVRAPAGPVGAEAPGTLVCGPVTLLPGERRVTVDGAEID
                     LTATEFDLLAWLMRRPGQVYSRSQLMEHVWGYDSAAGQRTVDVHVAQLRAKLAGADVI
                     RTVRGVGYAAQTDRP"
BASE COUNT          247 a          594 c          550 g          204 t
ORIGIN      
        1 ccgcgaagag tgcaccgaga ccggcgagga cgagcagcgg accataggag acgaacgggg
       61 cgatcgacca gcacacggcc tgggccacga tcgagggcgt catggtccgg cgcagcccga
      121 tccggtcgag caggcggccg cgccacgggc ccgagaccgc cagggccacg gtcacgacca
      181 tcgtcgccac cccggcggcg ccatacgtct gccgcagggt gctgacgata tggatcgtga
      241 ggatgacgct cgcggcgaac atcgggatcc tgatgacgaa gccgagcagc atcgccgacc
      301 gggcctcacg cacggccagg acgtcccggt aggccgtcag ctccacggcg caagcctcgc
      361 acgcccctgc gacacgaggt ggcccgcggg ggcaggcccg ctgacggcgt atgccgtgcc
      421 cgccccccgc gggcacggca taccggcttc gccatgggtc cgggcgccgt cgagggagca
      481 gcctgaacct attccggttc cgcacgcaga tccgcaaccc ggcaaccatc ctgtacaaca
      541 ccggacctat caccgacatc acgtcggcga gctggaaccg tccccagttc tactccgtca
      601 cacgcatcgc gcgaggccac ccgccggtcc tggcgaaggg cctgggtgcg ctccggccct
      661 tcaccatggc ccatctgatc cctcttgccg ccacggcagg agtcaacggc gtgtggtcga
      721 cggccagccg gaggacggcg acgatccggg tggaaggcaa gggcaagaag gtcgcggcgc
      781 gactagattg gcgcgggtga gcacgcaggg gatggtcctc gtcgtcgagg acgaccacgc
      841 gatcgccacc atgctcaccc tcgccctgcg caaggcgggg tatgacgcgc aggtcgccag
      901 cgatggcgac gcggcgctgg cggctgtggc agccatccgt ccggacgcgg tcctgctcga
      961 cgtcgggctc cccggccgcg acgggatcga cgtgtgccgg cagctccggg cgcgaggcga
     1021 ctggaccccg gtgctgttcg tcacggcccg cgacgacgag atcgaccgga tcgtcggcct
     1081 ggagctcggc gccgacgact acatcaccaa gccgttctcc ccgcgcgagg tcgtcgcccg
     1141 tatcgggacc gtcctgcgcc gcgtccgtgc accggcgggc ccggtcggcg ccgaggcgcc
     1201 cgggaccctg gtctgcgggc cggtgacact actccccggt gagcgccggg tcactgtcga
     1261 cggggccgag atcgacctga ccgcaacgga gttcgacctc ctcgcctggc tgatgcgtcg
     1321 ccccggccag gtctacagcc gatcccagct catggagcac gtctggggct acgactctgc
     1381 ggcgggacag cgcaccgtcg acgtccacgt cgcgcagctc cgggccaagc tcgcgggcgc
     1441 cgacgtcatc cgcaccgtcc ggggcgtcgg gtacgcggcg cagacggacc gtccgtgaca
     1501 ctgccacctg tgacactgtg ccccccgtga ccccaccccc gcacgggcag ccgcgccgcc
     1561 atacgggcct cgcggttcgt cttgccgtgc tcacc
//
LOCUS       DATAIX010000052         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_2626, whole genome shotgun sequence.
ACCESSION   DATAIX010000052 DATAIX010000000
VERSION     DATAIX010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2626"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VHM65_00885"
     CDS             complement(<1..352)
                     /locus_tag="VHM65_00885"
                     /inference="COORDINATES: protein motif:HMM:NF040294.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5998 family protein"
                     /protein_id="HEX2704287.1"
                     /translation="MPGRSGVSAGSRLPKDLLSSIERAGYYPALVADVVESALAGDDT
                     VAHLVHQETTFDASSVRRHITVLALTRAKLVVAHADDFSDGEPHEAVATATSETIPIG
                     AVRGVMVTHVVPSPD"
     gene            complement(373..>1569)
                     /locus_tag="VHM65_00890"
     CDS             complement(373..>1569)
                     /locus_tag="VHM65_00890"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX2704288.1"
                     /translation="GQRIAVVTNSVSLGAITAQSCLSWGLEVTHGPITLAADASVADY
                     VTALESAFADDAVDSVVTCFIPALVALNEDVTGAVKAISASSVKPCVATFLGMFGVTE
                     RADGVSPHAPGQTRALPVFAMPEDAVRALAAATRYSQWRAKDHGTVLDPPGVHRAAAR
                     SFVEDLLEATPKGRELSLTEATELLAAYGIRLWPSRHVFDADAAVAAGDELGYPVIVK
                     AASEVLRHQPGLRAVQGDRTSAEDVRWAMGMLEDRLGGFASEGVFVQRMATPGVACVL
                     SAIEDPLFGPVLSFSVAGPATDLLGDIGHRIAPLTDVDIGDLLDSIKAAPLLKGHRGA
                     EPVDRVALAELVARVSALADDLPEVEVVELNPVNAFAGGVDVLGARVVVGPEPNRPDA
                     HRRALT"
BASE COUNT          241 a          542 c          575 g          211 t
ORIGIN      
        1 ggtcgggcga gggcacgacg tgggtcacca tgacgccgcg aacggccccg atcgggatcg
       61 tctcgctggt cgcggtggcc acggcttcgt gcggctcgcc gtcggagaag tcgtcggcgt
      121 gggcgacgac gagcttggcc cgggtgagcg cgaggaccgt gatgtgccgg cgcaccgagc
      181 tcgcgtcgaa cgtcgtctcc tggtggacca ggtgggcgac ggtgtcatca cccgcgagcg
      241 cggactcgac gacgtcggcg acgagggctg ggtagtatcc cgcgcgctcg atcgaggaca
      301 gcaggtcctt gggcaggcga gagccggcgg agactccact acgaccaggc attgcgctat
      361 cttcccacgc cgtcaggtca aggcccgccg gtgcgcgtcg gggcgattgg gttccggccc
      421 cacgacgact cgagcaccca ggacgtcgac accaccggcg aaggcgttga ccgggttgag
      481 ctcgaccacc tcgacctcgg gcaggtcatc ggccagcgcg ctgacccggg ccacgagctc
      541 ggccagagcc acccggtcga ccggctcggc cccgcgatgg cccttgagca acggcgccgc
      601 cttgatggag tcaagcaggt cgccgatgtc gacatcggtg agcggcgcga tccggtgacc
      661 gatgtccccg agcaggtcgg tcgccgggcc cgcgaccgag aagctgagca ccggcccgaa
      721 cagcgggtcc tcgatcgcac tgaggacgca cgcgaccccg ggcgttgcca tccgctggac
      781 aaagaccccc tccgaggcaa acccgccgag ccggtcctcg agcatcccca tggcccagcg
      841 cacgtcctcg gcggaggtgc ggtcgccttg gaccgcccgc aggccgggct ggtggcgcag
      901 gacctcggag gcggccttga cgatcacggg gtagcccagc tcgtcgcccg ccgcgacggc
      961 tgcgtcggca tcgaagacgt gccggctcgg ccacaacctg atgccgtatg ccgcgagcag
     1021 ttcggtcgcc tctgtgaggg acagctctcg gcccttcggg gtcgcctcga gcaggtcctc
     1081 gacgaaggag cgggcggccg cacgatggac tcctggcggg tcgagaacgg tcccgtggtc
     1141 cttggcgcgc cactgggagt aacgcgtcgc ggctgccagc gcccggaccg cgtcctcggg
     1201 catcgcaaag accgggagcg cgcgggtctg gcccggggca tggggcgaga cgccgtccgc
     1261 gcgctcggtc accccgaaca tgccgaggaa ggtcgcgacg cacggtttca ccgaggacgc
     1321 cgagatggcc ttgaccgccc cggtgacatc ttcgttcagg gccaccagcg ccgggatgaa
     1381 acaggtgacg acgctgtcga cggcgtcgtc ggcgaaggcc gattcgaggg cggtgacgta
     1441 gtcggcgacc gaggcgtccg ccgccagtgt gatcgggccg tgggtgacct ccaagcccca
     1501 gctcaggcac gactgggccg tgatcgcgcc gagcgagacc gagttcgtca cgaccgcgat
     1561 ccgctggcc
//
LOCUS       DATAIX010000053         3130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_32790, whole genome shotgun sequence.
ACCESSION   DATAIX010000053 DATAIX010000000
VERSION     DATAIX010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32790"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..962
                     /locus_tag="VHM65_00895"
     CDS             252..962
                     /locus_tag="VHM65_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704289.1"
                     /translation="MTYTLFGSLTAVLCQGWIEPLTGLEIQAHVQRADAVDTPAQGQL
                     VGTAAIHARGAYRLVLASTYYSGGLVDLSLVMAAPPREPFERGTPPLHVGLGTITPLW
                     RMETGRAVADWDCELAQQQWRSMLRRFGVWTICGRLVSATGATVTAYDADWIQDDLLG
                     ESATDAEGRFLITYVAADFRRTPLSPVITMGSGGPDVYFTAQHGGRSILHESRAEGRR
                     AERRDIDDCYCVELEADV"
     gene            complement(975..2318)
                     /gene="ccrA"
                     /locus_tag="VHM65_00900"
     CDS             complement(975..2318)
                     /gene="ccrA"
                     /locus_tag="VHM65_00900"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR01751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HEX2704290.1"
                     /translation="MTMQPIRDAILAGDRTEATYTALPMPTSYRAVTVHKDETAMFEG
                     MASRDKDPRRSLHLDDVPVPELGPGEALVAVMASSINYNTVWTSIFEPVSTFGFLERY
                     GKTSPWAKRHDLPYHIVGSDLSGVVLRTGPGVHLWKSGQEVVAHCLSVELEHHDGHND
                     TMLDPEQRIWGFETNFGGLAELAIVKSNQLMPKPEHLTWEEAASPGLVNSTAYRQLIS
                     RNGAGMKLGDRVLVWGASGGLGSYATQMALAGGGQPICVVSSPEKAAICRAMGAELVI
                     DRAAEGYKFWKDERTQDMKEWRRLGAKIRELTGGADPDIVFEHPGRETFGASVFVTRR
                     GGTIVTCASTSGFMHEYDNRYLWMNLKRIVGSHFANYREAWEANSLVRRGLIHPTLSK
                     AYALADVGQAAYDVHRNRHQGKVGVLALAPEEGLGVTHHERRAELSGAINRFRDA"
     gene            complement(2406..>3130)
                     /locus_tag="VHM65_00905"
     CDS             complement(2406..>3130)
                     /locus_tag="VHM65_00905"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX2704291.1"
                     /translation="RALARVQGGPAYPFYDTPVSGLRPDLSRPGAQRGAVIWAGSAAT
                     RRLALTFDDGPHPNWTPRVLAALARHNVKATFFVRGDNLTAHGDLLTGTGHEVANHTW
                     DHPDLAAADYRACRDQLSRTSAEIARRFGRPPALFRPPYGHLGGAAMLAAAELGLTTV
                     LWSAQMRESRYVAEPAGIVGDIAANVGPGAIILAHDTGPADRLVTIDHLDDILTRLRD
                     DGYALTTVSELAGLGQEPTTAT"
BASE COUNT          428 a         1065 c         1148 g          489 t
ORIGIN      
        1 cagatggtgc ctcggccgtc ggccctgtcg gcgggttgcc gccctcggcc ggcccggtcc
       61 gatcgtacgc agtgagccgg ggccggcggg cggcgcgcag catctcgcgc tgacgccgcc
      121 ggatctcacg gagccgggcg agcatcacct cacctccctg cgctgagcct agcccggcac
      181 gccgtgctgg acctgggccg acacggtggg gcatagtgcc ccttggccgg ccgggagcga
      241 agggggagtc cgtgacatac acgctgttcg ggtcgttgac cgccgtgctg tgtcaagggt
      301 ggatcgagcc cctgaccggc ctggagatcc aggctcacgt ccagcgtgcc gatgccgtcg
      361 acacgcccgc gcagggccag ctcgtcggga ccgcggcgat ccacgcgcgg ggggcctacc
      421 ggctcgtcct ggcctcgacg tactactccg gtggcctggt ggacctctcg ctggtcatgg
      481 cggcgccgcc gcgggagccg ttcgagaggg ggacaccgcc gctgcacgtc ggcctgggca
      541 ccatcacccc gctgtggcgc atggagaccg gccgggccgt ggccgactgg gactgcgagc
      601 tggcgcagca gcagtggcgc tcgatgctgc gccgtttcgg ggtctggacg atctgcgggc
      661 ggctcgtgtc ggcgaccggc gccacggtga cggcatacga cgccgactgg atccaggatg
      721 acctcctcgg cgagtccgcc accgacgccg agggacgctt cctcatcacg tatgtcgcgg
      781 ccgacttccg ccgcaccccg ctctcgccgg tcatcaccat ggggtccgga ggcccggacg
      841 tctacttcac cgcgcagcac ggcggtcgct cgatcctgca cgagagcagg gccgaaggcc
      901 gccgcgccga gcgacgcgac atcgacgact gctactgcgt cgagctcgag gccgacgtct
      961 gacccgtgcc gcgctcaggc gtcccggaac cggttgatgg cccccgacag ctcggcccgg
     1021 cgctcgtgat gggtgacccc caggccttcc tcgggggcca gggcgagcac gccgaccttg
     1081 ccctggtgcc tgttgcggtg cacgtcgtac gccgcctgcc cgacatcggc cagggcatag
     1141 gccttgctca gcgtcgggtg gatgaggcca cggcggacga ggctgttggc ctcccacgcc
     1201 tcgcggtagt tcgcgaagtg cgagccgacg atccgcttga ggttcatcca caggtagcgg
     1261 ttgtcgtact cgtgcatgaa gcccgacgtc gacgcgcacg tcacgatggt gccgcccctg
     1321 cgggtgacga agacgctggc cccgaacgtc tcacgccccg ggtgctcgaa gacgatgtcg
     1381 gggtccgcgc cgccggtgag ctcgcggatc ttggcgccga ggcggcgcca ctccttcatg
     1441 tcctgcgtcc tctcgtcctt ccagaacttg tatccctcgg ccgcacggtc gatgacgagc
     1501 tcggccccca tggcgcggca gatcgcggcc ttctccgggc tcgacacgac gcagatcggc
     1561 tggccacctc cggccagggc catctgggtg gcgtacgagc cgaggccacc gctggcaccc
     1621 cagacgagga cgcggtcgcc gagcttcatc ccggcgccgt tgcgtgagat gagctgccgg
     1681 taggccgtcg agttcaccag accgggagag gcggcttcct cccaggtgag gtgctcgggc
     1741 ttgggcatga gctggttgga cttgacgatg gcaagctcgg ccagaccgcc gaagttcgtc
     1801 tcgaagcccc agatccgctg ctcggggtcg agcatcgtgt cgttgtgccc gtcgtggtgt
     1861 tcgagctcga ccgagaggca gtgggcgacg acctcctggc cggacttcca caggtgcact
     1921 cccgggccgg tccgcaggac cacgcccgac aggtccgaac cgacgatgtg gtacggcagg
     1981 tcgtgccgct tggcccacgg ggaggtcttg ccgtagcgct cgaggaagcc gaaggtcgac
     2041 accggctcga agatcgaggt ccacaccgtg ttgtagttga tcgagctggc catgacggcg
     2101 acgagggcct cacccgggcc gagctcgggc acgggcacgt catcgaggtg cagggagcgg
     2161 cgcgggtcct tgtcgcggct cgccattccc tcgaacatcg cggtctcgtc cttgtggacg
     2221 gtcacggccc ggtatgacgt cggcatgggc agggcggtat aggtcgcctc ggttcggtca
     2281 ccggcgagga tggcatcacg gatcggctgc atcgtcatac gtcgcaaggt acccgggtct
     2341 cccggcccag ggtcaggcgg ggaggccccg gccacacggt gaccttgctc acccagcggg
     2401 gtgagtcacg tggcggtcgt tggttcttgg ccgagaccag cgagctcgct caccgtcgtc
     2461 agtgcgtagc cgtcgtcccg caaccgggtg aggatatcgt ccaggtggtc gatcgtcacg
     2521 agccggtcgg ccgggccggt gtcgtgggcg aggatgatag ctcccgggcc gacgttcgcc
     2581 gcgatatcgc cgacgatgcc agccggctca gcgacatacc tgctctcgcg catctgggcg
     2641 ctccacagca ccgtggtgag cccgagctcg gcggctgcga gcatcgctgc gccgccgagg
     2701 tgaccgtacg gcggccggaa caaggccggg gggcgcccga accgccgggc gatctccgca
     2761 ctggtgcgag acagctggtc ccggcaggcg cggtagtcgg cggcagcaag gtccgggtgg
     2821 tcccaggtgt ggttggcgac ctcgtggccc gtgccggtga gcaggtcccc atgggcggtg
     2881 aggttgtcgc cccggacgaa gaacgtggct ttgacgttgt gccgggccag ggccgcaagg
     2941 acccgcggtg tccagttcgg gtgggggccg tcgtcgaagg tcagcgccag ccggcgggtg
     3001 gctgcgctgc ccgcccagat gacggcgccg cgctgcgcgc cggggcggct gaggtcgggc
     3061 cgcagtccgg acaccggggt atcgtagaag ggataggccg gtccgccctg gacccgggcg
     3121 agggcgcgct
//
LOCUS       DATAIX010000054         3688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_32835, whole genome shotgun sequence.
ACCESSION   DATAIX010000054 DATAIX010000000
VERSION     DATAIX010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32835"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VHM65_00910"
     CDS             <1..197
                     /locus_tag="VHM65_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704292.1"
                     /translation="LRPASRRQIHAVTTPDLQRVPAVKATLDALSEAALRGHGPSVPW
                     PRPVMAVGPAGGGTDWIATD"
     gene            complement(241..2646)
                     /locus_tag="VHM65_00915"
     CDS             complement(241..2646)
                     /locus_tag="VHM65_00915"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX2704293.1"
                     /translation="MTPNARQGAAVEVSARRADVRLLVPGLWGWALVAAVSGLDVGQQ
                     VLVLVASAVVTVWGLWLMSVRAARGRPGSLPGLLATTGAVSALLVGALIGHRASADAG
                     PVAALAAGRAVVTVTARVQGDPRSLAPTLESARAPRVVVALLVTQIDGRGTRTEVRSP
                     VLAFGGPDLASVRWGQAVVATGRLRAADPGDNQVALLDVRGAPRVVEPEPALLGAAEV
                     VRERFRTATSGLAPDARGLVPALVIGDTSRTPSNLTAAMLTAGMTHLSAVSGSNVSLV
                     LTAWLGLCRLAGVPRRWRPGFAGVGLVLFVVLARPEPSVVRAAVMGAVGLLGLSTSRR
                     GAGLPALATAIVALLVWDPWLARSYGFALSCLATLGLVLFARPWGEAIGARLPARIAG
                     WGPALAIPVIAQVMCAPVVVLLQGSVSVVAIPANLAAAPFVAPATFAGLATAVVSVVW
                     PAAGAVVAWGAALPALAIAWVARTAATLPGATLPWPDGAGGALLLVGVSLAAVLSGPW
                     VRSHARSRPLLAVAVVVLGAATATPTLPFTWPPPGWTAVFCDVGQGDAAVVATAPGHA
                     LVVDAGPDPRLVDGCLRRLGIEVVDAIVLTHFHADHVAGLSGVLGARPVGEILVSPVP
                     DPPYEAHEVSRLAQEAGVPVRTVYAGDDLRWGSHEVHVWWPARALASGSVPNNGSLVV
                     TLRGPTLNALMLGDIEREASQELVGLLRRAPPAELAPFDVVKVAHHGSANRDEELMAL
                     VHAPTAVISVGADNDYGHPAWSTLRELVSLGYAVARTDRDGDIAISREPGAAPVLTRR
                     GPG"
     gene            complement(2643..3464)
                     /locus_tag="VHM65_00920"
     CDS             complement(2643..3464)
                     /locus_tag="VHM65_00920"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX2704294.1"
                     /translation="MLDATLRGGWALEPSTTAGEAGPEAGGGPAEPGLPTRRDRRSVA
                     RPPVFSVPGPLRHVRVRTTRRTVLALAAVTLAALVLFGVRVWLAQAAASPQPVPGAVA
                     TSGEGTPSGLVARSVPPAFAPRTSAASTTKEQLVVHVVGSIAHPGIVRVPPGSRVIDA
                     ITAAGGPTTHADLSRVNLARLVVDGEQIAVPRPGEVVTGPLGATPSGAAGARVDLNAA
                     DLAALDALPGVGPVLAQRILDWRADHGRFSTVDELAEVSGIGDKLLAQLRPKVQV"
BASE COUNT          578 a         1398 c         1329 g          383 t
ORIGIN      
        1 gactccgacc cgcatcacga cgccagatcc atgccgtcac tacgccagac ctgcagcggg
       61 ttcccgctgt caaggccacc ctcgacgccc tgagcgaggc ggcgctgcga ggacacggtc
      121 cttcggtgcc ctggccccga ccggtcatgg cggtcgggcc ggccggcggc ggcaccgact
      181 ggatcgccac cgactgaccg gcaccgactg accggcaccg cctgaccggc cgggttgctc
      241 tcaaccggga ccgcgcctcg tcaggacggg cgccgcaccc ggctcccggg agatggcgat
      301 atcgccgtcg cgatcggttc gggcgaccgc gtatccgagc gagaccagct cgcgcagcgt
      361 ggaccaggcc gggtgcccgt agtcgttgtc ggcccccacg ctgatcaccg cggtcggagc
      421 atggacgagc gccatgagct cttcgtcgcg gttggctgag ccgtggtgag cgaccttgac
      481 gacatcgaac ggggcgagct cggccggtgg agcccgtcgc agcagcccga ccagctcctg
      541 ggacgcttcc cgctcgatat cgccgagcat caacgcgttg agggtcggcc cgcgcagcgt
      601 caccacgagc gagccgttgt tgggcaccga cccggaggcg agcgcccggg cgggccacca
      661 gacatgcact tcgtgcgacc cccaccggag gtcatcgccg gcatacaccg tgcggaccgg
      721 cacacccgcc tcctgcgcca gccggctcac ctcgtgggcc tcgtacggcg ggtcgggcac
      781 cggcgagacg aggatctccc ccacgggccg ggcaccgagg acaccgctca ggccggcgac
      841 gtggtcggcg tggaagtggg tgagcacgat ggcgtcgacg acctcgatcc cgagccggcg
      901 caggcatccg tcgaccagtc tcggatccgg gcccgcatcg acgaccagcg catgccccgg
      961 tgctgtcgcc acgacggccg cgtcgccctg tccgacgtca cagaagaccg ccgtccagcc
     1021 tggcggcggc caggtgaacg gcagggtcgg agtggcggtt gccgcgccga ggaccaccac
     1081 cgccaccgcg agcaggggcc gggaccgggc gtggcttcgc acccagggcc cgctgagcac
     1141 ggcggccagg ctgaccccga cgagcaggag cgcgccccct gcgccgtccg gccatggcag
     1201 ggtcgccccg ggcagcgtgg cggcggtccg ggcgacccac gcgatcgcga gagctggcag
     1261 ggccgccccc cacgccacga ctgcgccggc ggcgggccac accaccgaca ccacggcggt
     1321 cgccagcccc gcgaacgtgg ccggcgcgac gaacggggcc gccgcgaggt tggccgggat
     1381 ggcaacaacc gagaccgacc cctgcagcag gacgacgacc ggggcgcaca tgacctgggc
     1441 gatcacgggg atcgccaggg ccggccccca cccggcgatc cgggcaggaa ggcgtgcccc
     1501 gatcgcctcg ccccacggcc gggcgaacag caccagcccg agggtggcca ggcaggacag
     1561 ggcaaagcca taggagcgag cgagccaagg gtcccagacc agcaacgcca cgatggccgt
     1621 ggccagcgcc ggcagcccgg caccccgccg cgaggtggac aggccgagca gcccgacagc
     1681 gcccatgacg gccgcgcgca ccacgctcgg ctcgggccgg gccagcacga cgaacaggac
     1741 caggccgaca ccagcaaacc ccggccgcca gcggcgtgga accccggcaa ggcggcacag
     1801 tcccagccag gcggtgagca ccaggctgac gttggacccg ctgacggcgc tcaggtgcgt
     1861 catccccgcc gtcagcatgg ctgcggtcag gttcgacggg gtccgtgacg tgtcaccgat
     1921 cacgagtgcg ggcaccagcc cccgcgcgtc cggggccagg ccagaggtcg ccgtgcggaa
     1981 ccgctcgcgc accacctcgg ccgccccgag cagagccggc tccggctcga cgacacgagg
     2041 tgccccgcgg acgtcaagca gagccacctg gttgtcgccc gggtccgccg cccgcaaccg
     2101 tcccgtggcc acgaccgcct ggccccaccg taccgaggcc aggtcggggc caccgaaggc
     2161 aaggacgggg ctcctgacct cggtgcgcgt gccacgcccg tcgatctggg tgacgagcag
     2221 cgcgacgacg acccgagggg cgcgcgcgga ctcgagggtc ggtgcaaggc tccgggggtc
     2281 gccctggact cgcgccgtca cggtgaccac ggcacggccg gcggccagcg ccgcgacggg
     2341 tccagcgtcg gcgctggccc gatggccgat cagggcaccg accagcagag ccgacaccgc
     2401 tcccgtggtt gccagcagtc cgggcagcga gccgggacga cctcgggccg ctcgcacgct
     2461 catcagccac agtccccaaa ccgtgacgac ggcagacgcc acgagcacca gcacctgctg
     2521 gccgacgtcc agtcctgaga ccgcggccac cagggcccac ccccacagcc ccggaacaag
     2581 gagcctgaca tcggctcgcc gcgctgagac ctcgaccgct gccccctggc gagcgttcgg
     2641 cgtcatacct ggaccttggg acggagctgg gcgagcagct tgtccccgat cccgctcacc
     2701 tcggcgagct cgtcgacggt gctgaaccgt ccgtggtcgg ctcgccagtc caggatgcgc
     2761 tgcgccagga ccggcccgac tcccggcaac gcgtccaggg cagccaggtc ggccgcgttg
     2821 aggtccaccc gagcgccggc agcgccggag ggcgtggcac ccagtggccc cgtgacgacc
     2881 tccccgggtc gagggacggc gatctgttca ccgtcgacga cgagccgggc caggttgacc
     2941 ctggacaggt cagcatgcgt ggtcgggcct ccggccgcgg tgatggcgtc gatgacccgt
     3001 gaacccggcg gaacgcgcac gatccccgga tgggcgatgg acccgacgac atgcacgacg
     3061 agctgctcct tggtggtcga cgcagcactc gtcctcggcg cgaaggcggg ggggacggac
     3121 cgcgcgacca gaccactggg agtcccctcg ccgctggtag cgacggctcc ggggacgggc
     3181 tgcggcgaag cagcggcctg ggcgagccag acccggaccc cgaacagcac cagggcagcc
     3241 agtgtcaccg cggcgagcgc gagcacggtg cgacgggtag tcctgactct cacgtggcgc
     3301 agcggaccgg gaacgctgaa caccggcgga cgcgccaccg accgtcggtc gcgtcgcgtg
     3361 ggcagccctg gctcggccgg gccaccaccg gcctcgggac cggcctctcc tgcggtcgtc
     3421 gacggctcga gcgcccaccc gccgcgcagg gtcgcgtcca gcatgggagg tgcctcggcg
     3481 ggctccgccg gccgcgaggg aacccagccc atcgcaggtc cgggggacgc gcgagggtga
     3541 ccgatcagcc cgggcagacg agctggcggg atcgctgggg caggagcacg cgccatacca
     3601 gcaccgtagg tcggccatcc cgccaaccgg gagacaccgc gtgccgcctg gggacggtgg
     3661 gactccgacg cctgccgctg tggacaac
//
LOCUS       DATAIX010000055         3466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_152063, whole genome shotgun sequence.
ACCESSION   DATAIX010000055 DATAIX010000000
VERSION     DATAIX010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152063"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /gene="sufB"
                     /locus_tag="VHM65_00925"
     CDS             complement(<1..753)
                     /gene="sufB"
                     /locus_tag="VHM65_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX2704295.1"
                     /translation="MSTIEELNPGLDGIGRYEYGWSDSDTAGATARRGLNEDVVRDIS
                     ARKNEPAWMLDLRLKGLRLFDRKPMPTWGSDLTGIDFQNIKYFVKSTEKQATSWEELP
                     EDIKNTYDRLGIPEAEKQRLVGGVAAQYESEVVYHQIREDLEAKGVIFVDTDTGLREY
                     EDVFKEYFTSVIPAGDNKFAALNTAVWSGGSFIYVPPGVHVDIPLQAYFRINTENMGQ
                     FERTLIIADEGSYVHYVEGCTAPIYKSDSLHSA"
     gene            complement(750..1523)
                     /locus_tag="VHM65_00930"
     CDS             complement(750..1523)
                     /locus_tag="VHM65_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2704296.1"
                     /translation="MGVVFIQDAVFTQDAALSDGPAPGGGLESRTRERVLHAISSLGP
                     VTAAALGTRLGLTATAVRRHIDNLVEAGLIAEHEMVNIRRGRGRPARSYVMSDSGHRA
                     LGSDYDHLAVDALRFLADTAGPEAVATFAQRRLASTEARYGAQLASVGADPAARAQAL
                     VGLLNADGFAASARPVAGGASPLTGVQICQGHCPVQHVAAEFPQLCDAETESFSRLLG
                     VHVQRLATLAHGEHVCTTFVPTSASGATSAAAPNERPSR"
     gene            complement(1592..2800)
                     /locus_tag="VHM65_00935"
     CDS             complement(1592..2800)
                     /locus_tag="VHM65_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704297.1"
                     /translation="MDLQPRGIETVKALLAKGVDIPNPLSLDIGPEVEVEHISGEGVR
                     LYPGCRIYGARTVICAGVQLGAEGPVTVKDARLGPGAELKAGYAADSVFLERSAMGWG
                     GHVREGCLLEEEASAAHCVGLKQTILFPFVTLGSLVNFCDALMAGGTSRRDHSEVGSS
                     YIHFNFTPAGDKTTPSLFGDVPRGVMLNQPPIFLGGQGGAVGPVRVGYGSVVAAGSVL
                     RSDVAAGQLAMPGPLPSMQVKVPPADYRSANRIVAKNLGYLANLCALQAWYRHVRQPF
                     FAASPLGALVYAGAVDMLALATKERAARLTAMTAKVSDDGGPRTQLRERIGEVLAAYA
                     ETPAQPPDAALLTGLRQAAGDGVGYVAAVQSLGPAQVEAGSAWLQGIVDGLWATSTKL
                     LPALVRMPRP"
     gene            complement(2805..3200)
                     /locus_tag="VHM65_00940"
     CDS             complement(2805..3200)
                     /locus_tag="VHM65_00940"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX2704298.1"
                     /translation="MDRTMRVERLGGDRYSIDVRGHQLTVDQPESDGGEDLAPTPTEL
                     FVAGLASCVAFYAGRFLKRHDIPADGLAVDAAYEFASKPARVAEVTLTVHAPAGLPAE
                     RRDALLAVASHCTVHNTVMSEPEITVTLV"
BASE COUNT          497 a         1204 c         1266 g          499 t
ORIGIN      
        1 cgcgctgtgc aggctgtcgg acttgtagat cggggcggtg cagccctcga cgtagtgcac
       61 gtaggagcct tcgtcggcga tgatcagggt ccgctcgaac tggcccatgt tctcggtgtt
      121 gatccggaag taggcctgca gagggatgtc gacgtgcacg cccgggggca cgtagatgaa
      181 cgagcctccc gaccacaccg cggtgttcag cgccgcgaac ttgttgtcgc cggcggggat
      241 gaccgaggtg aagtactcct tgaagacgtc ctcgtactcg cgcaggccgg tgtcggtgtc
      301 gacgaagatg acgcccttgg cctcgagatc ctcacggatc tggtggtaga cgacttcgct
      361 ttcatactgt gcggccacgc cgccgacgag gcgctgcttc tcggcctccg ggatgccgag
      421 gcggtcgtag gtgttcttga tgtcctcggg gagctcctcc caggaggtcg cctgcttctc
      481 ggtggacttg acgaagtact tgatgttctg gaagtcgatc ccggtcaggt ccgaacccca
      541 ggtcggcatc ggcttgcggt cgaaaaggcg caggcccttg aggcgcaggt cgagcatcca
      601 cgccggctcg ttcttgcggg cggagatgtc gcgaacgacg tcctcgttga gcccgcggcg
      661 ggcggtggcg ccggccgtgt cggagtcgga ccatccgtac tcgtagcggc cgatgccgtc
      721 caggccgggg ttgagctctt cgatcgtgct catcgtgagg gcctttcgtt cggggcggcg
      781 gcggaggtcg caccgctagc ggaggtgggg acgaaggtcg tgcagacgtg ctcgccgtgc
      841 gcgagcgtgg caaggcgctg gacatggacg ccgagcagac gcgagaacga ctcggtctcg
      901 gcatcgcaca gctgggggaa ctcggccgcg acgtgctgga ccgggcagtg gccctggcag
      961 atctggaccc cggtgagcgg gctcgccccg cctgccactg gccgcgccga cgccgcgaac
     1021 ccgtcggcgt tgagcagacc caccagggcc tgggcgcggg ccgccgggtc ggcgcccacg
     1081 gaggcgagct gcgcgccata ccgtgcctcg gtggaggcga gccggcgctg ggcgaaggtg
     1141 gcgacggcct cggggccggc cgtgtcggcg aggaagcgca gggcgtcgac cgccaggtgg
     1201 tcgtagtccg agcccagggc gcggtggccg gagtcgctca tgacgtagga ccgggcgggc
     1261 cgtccgcgcc cacggcggat gttcaccatc tcgtgctcgg cgatgagtcc cgcctcgacg
     1321 aggttgtcga tgtgccgccg gaccgccgtg gccgtgagcc cgaggcgggt gcccagggcg
     1381 gccgccgtga ccgggccgag ggagctgatg gcgtgcagga cccgctcgcg ggtgcgcgac
     1441 tcgaggccgc cgcccggagc cgggccgtcg ctgagcgcgg cgtcctgggt aaacacggca
     1501 tcctggataa acacaacacc catcttacgt aattcacccg ggaaacccaa gcgagcacga
     1561 cgggcgagtg gtcagcgggg cgaaaggccc ctcacggacg cggcatccgc accagcgcgg
     1621 gcagcagctt ggtggaggtg gcccacagcc cgtcgacgat gccctgcagc caggccgacc
     1681 cggcctcgac ctgcgccggg cccagcgact ggacggccgc gacgtagccg accccgtcgc
     1741 cggcggcctg gcgcaacccg gtcagcagcg cggcgtccgg tggctgcgcc ggggtctcgg
     1801 cgtaggcggc cagcacctcg ccgatccgct cccgcagctg cgtgcgcggc ccgccgtcgt
     1861 cgctgacctt ggccgtcatc gcggtgagcc gggccgcccg ctccttcgtg gccagcgcga
     1921 gcatgtccac cgccccggcg tagaccaggg cgcccaacgg cgaagcggcg aagaagggct
     1981 ggcgcacgtg gcggtaccac gcctgcagcg cgcacaggtt ggcgaggtag cccaggttct
     2041 tggcgacgat gcggttggcg ctgcgatagt cggcgggcgg gaccttgacc tgcatcgacg
     2101 gcagcggccc gggcatcgcc agctgcccgg cggccacgtc cgaacgcagc accgacccgg
     2161 cggcgaccac cgagccgtac ccgacccgga ccggcccgac cgcgcccccc tgcccgccga
     2221 ggaagatcgg gggctggttg agcatcaccc cacggggcac gtcgccgaac agtgacgggg
     2281 tcgtcttgtc cccggcgggc gtgaagttga agtggatgta cgacgagccg acctcgctgt
     2341 ggtccctgcg gctggtcccg cccgccatca gggcgtcgca gaagttgacc aggctgccca
     2401 gggtgacgaa cgggaacaag atggtctgct tcagccccac acagtgcgcc gcgctggcct
     2461 cctcctcgag caggcagcct tcgcgcacgt gcccgcccca gcccatggcg gaccgctcca
     2521 ggaacaccga gtccgcggca tacccggcct tgagctcggc ccccggtccc agccgggcgt
     2581 ccttgaccgt gaccgggccc tccgcgccga gctggacccc ggcgcagatg accgtccggg
     2641 ccccgtagat ccggcacccg gggtagagac gcaccccctc gccgctgatg tgctccacct
     2701 cgacctccgg gccgatgtcc aggctcagcg ggttcgggat gtccaccccc ttggccagca
     2761 gcgccttgac cgtctcgatc cctcgcggct gaaggtccat gccgtcacac cagggtgacg
     2821 gtgatctcgg gctccgacat cacggtgttg tggaccgtgc agtgcgacgc gaccgccagg
     2881 agggcgtcgc gccgctcggc ggggaggccc gccggggcgt gcacggtcag ggtcacctcg
     2941 gccacccggg cgggcttgct ggcgaactcg tacgccgcgt cgacggccag cccgtcagcg
     3001 gggatgtcat ggcgcttgag gaaccggccg gcgtagaagg ccacgcagga cgccagaccg
     3061 gccacgaaca gctcggtcgg ggtaggcgcg aggtcctcgc caccatcgga ctcgggctgg
     3121 tccacggtca gctggtgacc ccggacgtcg atcgagtaac ggtccccgcc gagccgctcc
     3181 acgcgcatcg tgcgatccat gtcaggtcac ctgtcctttg ggggagccag cgccggctgc
     3241 ccgcaggagt gcgaccgccg gccagtctgc cgggatcagg gtagcaaata cccaggaggg
     3301 tatcgccagc ggggcccgag tcggtcctga ccgcccagca cctacactgc cggggtgccc
     3361 actccacccg agccagcggt cgtcgtcgcc gctgtgaccc gccggttcgg cgccgtccgg
     3421 gcctgcgcgg agatgacgtg gtcggcgccc gcgggtgcgg tcaccg
//
LOCUS       DATAIX010000056         2613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_32980, whole genome shotgun sequence.
ACCESSION   DATAIX010000056 DATAIX010000000
VERSION     DATAIX010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32980"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2613)
                     /locus_tag="VHM65_00945"
     CDS             complement(<1..>2613)
                     /locus_tag="VHM65_00945"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX2704299.1"
                     /translation="AVRSGDTPASERRAFATTPADILITTPESLFLMLTSSLRAALTG
                     VETVILDEIHAVAGTKRGAHLAVSLERLDALLERPAQRIGLSATVEPAEEVARFLAGG
                     RPVKIVRPPSTKAWDLDVVVPVEDMSALGTLTEDLSGPAAGDQRRASIWPHVEERIVD
                     LVSEHRSTLVFANSRRLAERLTARLNEIWAERQERAADETAELGPIEATATRTPAQVM
                     AQSGASAGTPGVLARAHHGSVSKEARSVIEEDLKAGRLPAVVATSSLELGIDMGAIDL
                     VIQVESPPSVGSGLQRVGRAGHQVGAVSRGVFFPKFRGDLVQCAVVVERMRAGAIETL
                     RVPANPLDVLAQQIVAMCALDEWAVDELETLVRRAAPFASLPRSALEAVLDMLAGRYP
                     SEEFAELRPRLVWDRVSGTLTGRRGAQRLAVTSGGTIPDRGLYGVFLASSEGAGRRVG
                     ELDEEMVYESRVGDVFTLGTSSWRIEDITHDRVLVTPAPGHPGRLPFWKGDALGRPSE
                     LGRAVGAFVREVTALGPAAALERVGAAGLDAWAADNLLTYLREQREATRVIPSDRTIV
                     VERFRDELGDWRVVVHSPYGGQVHAPWALCVSARMRERFGVDVQAMHGDDGIVLRLPD
                     LELEGGAELLDLVLLEPGDVHDLVTEQIGGSALFAARFRECAARALLLPRRRPDRRQA
                     LWQQRQRASALLQVASRYPAFPIVLEAMRECVQDVFDVPGLVELMTRVRSREITVVDV
                     ESAQPSPFARSLMFGYVAQFLYEGDSPLAERRAAALSLDPTLLSELLGRGEGVSLRDL
                     LDPAQVARTEAELQRLAEERRCRDAEDVADLLRHLGPLPVGAVAMRCQGGTEAVALGF
                     LEALQAQRRVIRVRI"
BASE COUNT          368 a          938 c          989 g          318 t
ORIGIN      
        1 gatcctgacc cggatcaccc gtcgctgggc ttgcagagcc tccaggaagc cgagcgcgac
       61 cgcctcggtc ccgccctggc accgcattgc gacggctccg acgggcagcg gcccgagatg
      121 gcggagcagg tcggccacgt cctcggcgtc gcggcaccgg cgttcctcgg ccaggcgctg
      181 cagctcagcc tcggtgcggg caacctgggc cgggtcgagc aggtcacgca gggagacgcc
      241 ttcgccgcgg ccgagcagct cggagagcag cgtcgggtcg agtgacaagg ccgcggcccg
      301 gcgttcggcg agcggtgagt cgccctcgta gaggaactgc gcgacatacc cgaacatcag
      361 cgagcgggcg aacggcgagg gctgcgcgct ctcgacatcg acgacggtga tctcccggga
      421 gcgcacccgg gtcatcagct cgacgaggcc ggggacgtcg aagacgtcct ggacgcactc
      481 gcgcatggct tccaggacga tggggaaggc ggggtaccgg ctggcgacct ggagcagtgc
      541 cgaggcgcgc tggcgctgct gccacagcgc ctggcgccgg tcgggtcgcc gccggggcag
      601 cagcagggcc cgcgccgcgc actcgcggaa ccgcgcggcg aacagcgccg accccccgat
      661 ctgctcggtg acgaggtcgt ggacgtcgcc aggctcgagg agcacgaggt cgagcagctc
      721 ggcgccgccc tcgagctcca gatccggcag ccgcaacacg atgccgtcgt cgccgtgcat
      781 cgcctggacg tcgacgccga agcgctcgcg cattcgcgcc gagacgcaca gcgcccaggg
      841 ggcgtgcacc tgcccgccgt acggggagtg gacgacgacc cgccagtcac cgagctcgtc
      901 gcggaagcgc tcgacgacga tggtgcggtc actgggtatg acgcgggtcg cctcgcgctg
      961 ctcgcgcagg taggtgagca ggttgtcggc ggcccaggcg tccagacccg cggccccgac
     1021 ccgctccagc gcggcggccg gcccgagggc cgtcacctca cggacgaacg ccccgacggc
     1081 ccggccgagc tcggagggcc gccccagggc gtcacccttc cagaacggca gacgcccggg
     1141 gtgcccggga gcaggggtga cgagaacccg gtcgtgggtg atgtcctcga tgcgccagct
     1201 cgacgtgccg agggtgaaga cgtccccgac ccgtgattcg tagaccatct cctcgtccag
     1261 ctcgccgacc cgccggcccg cgccctcgct cgaggcgagg aacacgccgt acaggccgcg
     1321 gtcggggatc gtgccgccgc tggtgacggc gagccgctgg gcgccccgcc gaccggtcag
     1381 ggtgccgctg acccggtccc acacgagccg cggtcgcagc tcggcgaact cctcgctcgg
     1441 gtagcgcccg gcgagcatgt ccaggaccgc ctcgagggcg ctgcggggca ggctggcgaa
     1501 cggcgcagcc cgccgcacga gggtctcgag ctcgtcgacc gcccactcgt cgagggcaca
     1561 catcgcgacg atctgctggg cgaggacgtc gagcgggttg gccgggaccc gcagggtctc
     1621 gatcgcccct gcccgcatgc gctcgacgac gaccgcgcac tggaccaggt cgccgcggaa
     1681 cttggggaag aagactcctc gcgagaccgc cccgacctgg tggcccgccc ggccgacccg
     1741 ctgcaggccg ctgccgacac tcggcggcga ctcgacctgg atgacgaggt cgatcgcgcc
     1801 catgtcgatg ccgagctcga ggctgctcgt ggcgacgacc gccggcaggc gcccggcctt
     1861 gaggtcctcc tcgatgaccg agcgcgcctc cttgctcacc gacccgtggt gcgcgcgggc
     1921 caggaccccc ggcgtcccgg cgctggcccc ggactgggcc atgacctggg caggggtgcg
     1981 ggtcgcggtg gcctcgatcg ggccgagctc ggcggtctcg tcggccgcac gctcctgacg
     2041 ctccgcccag atctcgttga gccgggcggt gagccgctcg gccaggcgcc gggagttggc
     2101 gaagaccagg gtcgagcggt gctcggagac gaggtcgacg atgcgctcct cgacgtgcgg
     2161 ccagatcgag gcccggcgct ggtccccggc tgccggccca gagaggtcct cggtgagcgt
     2221 gccgagcgcc gacatgtcct cgacggggac aacgacgtcg aggtcccagg ccttggtcga
     2281 gggcgggcgg acgatcttga ccggacggcc ccccgcgagg aagcgggcga cctcctcggc
     2341 gggctcgacc gtcgccgaga gcccgatccg ctgggcgggc cgctccagga gggcgtcgag
     2401 ccgctccaag gacacggcga ggtgggcgcc gcgtttggtg ccggccacgg cgtggatctc
     2461 gtcgaggatg accgtctcga ccccggtcag tgccgcgcgg aggctggagg tgagcatgag
     2521 gaagagcgac tcgggcgtcg tgatgaggat gtcggccggc gtcgtggcaa aggcccgccg
     2581 ttcggacgcg ggtgtgtccc cggaccgcac ggc
//
LOCUS       DATAIX010000057         1568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_32983, whole genome shotgun sequence.
ACCESSION   DATAIX010000057 DATAIX010000000
VERSION     DATAIX010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32983"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VHM65_00950"
     CDS             complement(<1..331)
                     /locus_tag="VHM65_00950"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HEX2704300.1"
                     /translation="MDQDLPSPAGGTAPIEDLLDVLDLTPLGTSTITVRGAGADDEPA
                     AEAFTAMVFVGRSQPQPHGRVFGGQVLAQSVMAAGRTVEALPSHAVSPRRLHSLHAYF
                     IRPGDDSL"
     gene            complement(436..>1568)
                     /locus_tag="VHM65_00955"
     CDS             complement(436..>1568)
                     /locus_tag="VHM65_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704301.1"
                     /translation="SPVAYYQQVGRAGRATTSADVLLLPGPEDRDIWRYFASASMPRQ
                     EQADAVLGALRGSAAPVSTAALETVVDIRRTRLELLLKVLDVEGAVSRATGGWTATGQ
                     DWVYDGERYARVAAAREHEQQLMLDYEHGPGCRMEFLQRALDDASAAPCGRCDRCTAP
                     WYPSSMPDTAVLAARARLAQVGVELEPRAMWPTGMGRLGVPRRGRIGADEQAEPGRAL
                     ARLTDLGWGQRLRELLRSPDPHPSAELVDACIEVLKGWGWARRPVAVAAIPSRSRPHL
                     GPALAAELARVGRLEWLGTLDLVDGGPVGAAGGNSAFRLAGVFGRLAVPAGMASRLAA
                     VGGPVLLVDDLAATRWTLTVAAAELRAHGAGAVLPFALALEA"
BASE COUNT          200 a          592 c          610 g          166 t
ORIGIN      
        1 gcagcgagtc gtccccaggc cggatgaagt aggcgtgcag cgagtgcagg cggcgcggcg
       61 acaccgcgtg gctgggcagg gcctcgacgg ttcgccccgc ggccatgacc gactgggcca
      121 gtacctggcc gccgaagaca cggccgtgcg gctgcggctg gctgcggccc acgaacacca
      181 tggccgtgaa ggcttcggcg gcgggctcgt cgtcggcgcc cgcgccccgc acggtgatcg
      241 tcgacgtgcc gagcggggtg aggtcgagga cgtcgagcag gtcctcgatc ggtgcggtgc
      301 ccccggccgg ggaaggcagg tcctggtcca catcgagaga ctatccggcc tcggcgcagc
      361 ctccggccac gcccgcaggg ccgcgttccc gtccggcccg ccgggccggg ttcccctccg
      421 gcccgcaggt ctgggctacg cctccagcgc gagggcgaat gggagcacgg cgccagcccc
      481 gtgcgcgcgg agctcggccg ccgccaccgt gagggtccag cgcgtggcgg cgaggtcgtc
      541 gacgagcagc acgggacccc ccacggcggc gagccgcgac gccatacctg cagggacggc
      601 gaggcgaccg aagaccccgg cgagccgaaa ggcgctgttg ccgcccgccg cgccgacggg
      661 cccgccgtcg acgaggtcga gggtcccgag ccactccagg cgcccgaccc gggccagctc
      721 ggcggccagg gccggaccga ggtgaggccg ggaccgagag ggtatggcgg ccacggcgac
      781 cggccgccgg gcccaccccc agcccttgag gacctcgatg caggcgtcca ccagctcggc
      841 cgagggatgc gggtccgggg agcgcaggag ctcgcgcagc cgctggcccc agccgaggtc
      901 ggtgagccgg gcgagcgcgc ggcctggctc ggcctgctcg tcggccccga tccgtccccg
      961 ccgggggacg cccagccggc ccatgcccgt cggccacatc gcccggggct cgagctcgac
     1021 gccgacctgg gcgagccgcg cccgtgccgc gaggacggcg gtgtcgggca tcgagctggg
     1081 gtaccacggc gcggtgcacc ggtcgcagcg gccacacggg gcggcgctgg cgtcgtcgag
     1141 ggcccgctgg aggaactcca tccggcagcc cgggccgtgc tcgtagtcga gcatgagctg
     1201 ctgctcgtgc tcccgcgccg cggccacccg ggcgtagcgc tcgccgtcgt agacccagtc
     1261 ctgccccgtc gccgtccacc caccggtggc ccggctcacc gccccctcga cgtcgagcac
     1321 cttgaggagc agctcgaggc gggtgcgccg gatgtcgacg acggtctcca gggcggccgt
     1381 cgacaccggc gcagccgagc cgcggagggc accgaggacg gcgtcggcct gctcctggcg
     1441 cggcatcgag gccgaggcga agtagcgcca gatgtcgcgg tcctcggggc cgggcagcag
     1501 aagcacatcg gcgctggtcg tggcccgtcc ggcccggccc acctgctggt agtaggccac
     1561 cggcgacg
//
LOCUS       DATAIX010000058         1656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_120583, whole genome shotgun sequence.
ACCESSION   DATAIX010000058 DATAIX010000000
VERSION     DATAIX010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120583"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VHM65_00960"
     CDS             <1..456
                     /locus_tag="VHM65_00960"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX2704302.1"
                     /translation="RVRPALAVLDRELGLTHALASQRLYTDGAEVLFDYADGGADSDT
                     ADAIWDLVVVRHGQRVFTEVVQSWLRRVTFRDDYAQALPLPGYDRARVIVDAGRSFGQ
                     PIFDHGRARLEDVLGMFRAGEPLDVVAAEYAVPLDELEEAVRVSLRRVA"
     gene            complement(562..1086)
                     /locus_tag="VHM65_00965"
     CDS             complement(562..1086)
                     /locus_tag="VHM65_00965"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEX2704303.1"
                     /translation="MSVNRPLDLLADAFGRVQECVADVVAGLSAEDLAYQPDPAANSI
                     GWLIWHLARVEDDHIAGIAGSEQVWARDGWAGRFALPYPVTTIGYGHSAADVAAFRVV
                     GPGLLAGYYDAVHARTLEVLRGLPDAGFDRIVDERWDPPVTAAVRLVSVVNDVTQHAG
                     QAAYVRGLLERRRS"
     gene            1122..>1656
                     /locus_tag="VHM65_00970"
     CDS             1122..>1656
                     /locus_tag="VHM65_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2704304.1"
                     /translation="MAQPSPPRGPPDVGRSAELTAALRRAGIHDADDSRLARALYSSD
                     ASLYRIPPLAVVRPRHRDEVAAVLSVCRDLGVPVTARGAGTSIAGNAIGPGVVLDYSR
                     HLNHVLHVDAEARTAVVEPGTIHATLQQQALARGLRFGPDPSTHSRCTVGGMVGNNAC
                     GSRALGYGRTSDNVLGLD"
BASE COUNT          231 a          607 c          582 g          236 t
ORIGIN      
        1 cgcgtccgtc cggcgctcgc cgtcctcgat agggagctcg ggctcacgca tgcgctggca
       61 tcacagcggc tctacaccga cggggccgag gtgctcttcg actacgccga cggcggtgcg
      121 gactccgaca ctgccgacgc catctgggac ctggtggtcg tgcggcacgg ccagcgggtc
      181 ttcaccgaag tggtccagag ctggctacga cgagtgacct tccgagatga ctacgcccag
      241 gcgcttcccc tacccggata cgaccgagcg agagtcatcg tcgatgcggg gcggagcttc
      301 ggccagccga tcttcgacca cgggcgcgcc cggctcgagg atgtgctcgg gatgttccgc
      361 gcaggcgagc cgctcgatgt cgtggccgcg gagtacgccg tgcctctcga cgagctcgaa
      421 gaggccgttc gggtatcgct gcgccgcgtg gcatgaccca ccgtgaacgc acaggcgccg
      481 gtcttcttcc ttgatcgaag tctgggccgc aagcaagtcc ccaacggtct ccgccggccg
      541 gggcggggga agcccgtcgg ctcagctgcg gcgccgctcg agcagacctc ggacgtaggc
      601 agcctggccg gcgtgctgag tgacgtcgtt gacgacgctg acgaggcgca ctgcggcagt
      661 cacgggaggg tcccagcgct cgtcgacgat ccggtcgaag ccggcgtcgg gcagcccgcg
      721 caggacctcc agggtgcgcg cgtgcacggc gtcgtagtac ccggccagca ggccggggcc
      781 gaccacccgg aaggccgcga catcggccgc gctgtgcccg tagccgatcg tagtgacggg
      841 gtagggcagg gcgaaccgcc cggcccagcc gtcacgcgcc cacacctgct cgctcccggc
      901 gatcccggcg atgtggtcgt cctcgacccg cgcgagatgc cagatcagcc agccgatcga
      961 gttcgccgcc ggatccggct ggtaggccag gtcctcggcg gagagtcccg ccacgacgtc
     1021 ggcgacgcac tcctggaccc gcccgaaggc gtcagcgagc aggtcgagtg gccggttcac
     1081 gctcatgggg gctccttggg gtcagtgcca tgcttgcagg tatggcgcag ccctcaccac
     1141 cccggggtcc gccggatgtc gggcgcagcg ccgagctgac ggctgccctt cgccgggccg
     1201 ggatccatga cgccgacgac tcgcgactgg cccgggcgct ctactcctcg gacgcctcgc
     1261 tctaccgcat cccgccgctg gccgtcgtcc ggccccggca ccgcgacgag gtcgccgccg
     1321 tgctgtccgt ctgccgagac ctcggtgtgc cagtgaccgc ccggggcgcg gggacgtcca
     1381 tcgcgggcaa cgcgatcggc ccaggcgtcg tcctcgacta cagccgccac ctcaaccatg
     1441 tgctccacgt cgacgccgag gcgcggaccg ccgtcgtgga gcccggcacc atccacgcga
     1501 cgctgcagca gcaggccctc gcgcgcgggc tgcgcttcgg tccggacccg agcacccact
     1561 cccggtgcac cgtcggcggg atggtcggca acaacgcgtg cgggtcgcgt gcgctcggct
     1621 acggcaggac gtcggacaac gtcctcggtc tcgatc
//
LOCUS       DATAIX010000059         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_33030, whole genome shotgun sequence.
ACCESSION   DATAIX010000059 DATAIX010000000
VERSION     DATAIX010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33030"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VHM65_00975"
     CDS             <1..273
                     /locus_tag="VHM65_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX2704305.1"
                     /translation="QSLLGDRVTDVVARQQSLRVDHFDTPAHFRDYFKTHYGPTIAVY
                     RSIAGEPGKVEALDQDLVALAQRFDVGVGTTVLDWEYLLVTASKRS"
     gene            506..1999
                     /locus_tag="VHM65_00980"
     CDS             506..1999
                     /locus_tag="VHM65_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2704306.1"
                     /translation="MPAADVSYDPRTGSPGGAEPWTSPEAGSAAVHAAAAAADAVAST
                     DPRLRQQWLGRVAAAIEADAQSLAALADEETGLGLPRLEGEARRAAAQARFYAQVGAE
                     GSWLGAALDTVPGPPVVDLRRVNRPVGPVAVFGASNFPFGFGLAGHDTMSAIAAGCPV
                     VAKAHPAHPRLGAALAAVVSGALAQAGAPDGTFALVRGFDAGLALVDAPEIAAVAFTG
                     SQAGGMALAARAAARPRPVPVYAEMGTVNPVVVTPAGAAARGDEIAQGFVASFTLGAG
                     QFCTKPGLILVPAGSGLAAAVAAAVTGAATAPLLTEAIATNARLGVERLVGAGGRVLA
                     VAPGTTVGYGVAPVVLAAALDDLVPGSPLLEECFGPVALVIEYAGLDQLLGSARRLQP
                     CLAAAIASRGPGDPAVPDLVAVLAKLAGRVIVDGWPTGVATTWAQHHGGPWPSTSRPD
                     ATSVGAAALARFVRPTAYQDVPEAALPEPIRRDNPWGVPQRVDGVLR"
BASE COUNT          244 a          827 c          807 g          290 t
ORIGIN      
        1 cagtcgctgc tcggcgaccg ggtcaccgat gtcgtcgccc gccagcagag cctgcgggtc
       61 gaccacttcg acaccccggc gcacttccgc gactacttca agacgcacta cgggccgacg
      121 atcgcggtct accggtcgat cgccggggag cccggcaagg tcgaggccct cgaccaggac
      181 ctcgtcgcgc tcgcccagcg cttcgacgtg ggcgtgggga cgaccgtgct ggactgggag
      241 tacctcctcg tcacggcatc gaagaggtcc tgacccgggc gccccccgag gcgtcagcgc
      301 accgtgctcg cggcggccgt tacatgcagg tcacgtcggg cctgtcacga catcttccgc
      361 tgagcgtttc gcaccagcga tactcagatg cgtaggcgga ccgcacagag acaggggccg
      421 tgaggcgacc ttaggttgcg ggtgaggagc ggtgtgctcg gtgagtgctc acccaccggc
      481 gaccgtcagg acgaggagga tgcccgtgcc ggctgccgac gtcagctacg acccgcgcac
      541 cggctcgccc ggaggcgccg aaccgtggac ctccccggag gccgggtcgg ctgcggtcca
      601 cgcggcagcc gccgcggctg acgccgtggc cagcaccgac ccccgcctgc gccagcagtg
      661 gctcggccgg gtcgccgctg cgatcgaggc cgacgcgcag tccctggccg cgctggccga
      721 cgaggagacc ggcctgggcc tgccccggct ggagggcgag gcccggcggg ccgccgcgca
      781 ggcccggttc tacgcacagg tcggggccga agggtcctgg ctcggggccg ccctcgacac
      841 ggtcccgggg cctcccgtgg tcgacctgcg ccgggtcaac cgccccgtcg gcccggtcgc
      901 ggtcttcggg gcgagcaact tccccttcgg cttcgggctc gccgggcacg acacgatgag
      961 cgccatcgcc gccggctgtc cggtcgtggc caaggctcac cccgcgcacc cgcggctggg
     1021 cgccgcgctc gccgcggtgg tcagcggcgc gctggcccag gccggggcac cggacggcac
     1081 ctttgccctc gtccgcggct tcgacgccgg gctggcactg gtcgacgccc cggagatcgc
     1141 tgcggtggcg ttcaccggct cccaggccgg tggtatggcg ctcgccgccc gggccgcggc
     1201 acggcctcgg ccggtcccgg tctacgccga gatggggacg gtcaacccgg ttgtcgtcac
     1261 cccggccggc gccgcggctc gcggcgacga gatcgcccag ggtttcgtgg cctccttcac
     1321 gctgggcgcg gggcagttct gcacgaagcc aggcctgatc ctcgtcccgg ccgggtccgg
     1381 cctggccgcc gccgttgccg ccgcggtgac cggcgcagcc accgcgccgc tcctgaccga
     1441 ggccatcgcc accaatgcac gcctcggggt cgagcggctc gtcggggcgg gcggccgggt
     1501 cctcgccgtg gcccccggga caaccgtcgg gtatggcgtc gcacccgtgg tgctcgcggc
     1561 cgccctcgac gacctggtgc cgggctcgcc cctgctggag gagtgcttcg gcccggtcgc
     1621 gctcgtgatc gagtatgccg gcctcgacca gctgctcggg tcggcgcgcc ggctccagcc
     1681 gtgtctggcc gccgccatcg cgtcccgcgg accgggcgac cccgctgtgc ctgacctcgt
     1741 cgccgtactg gccaagctgg ccggccgggt catcgtcgac ggctggccca caggcgtggc
     1801 gacgacgtgg gcccagcatc acgggggacc gtggccctcg acgtcgcgcc ccgacgccac
     1861 gagcgtggga gccgcggccc tcgcgcggtt cgtgcggccg acggcctacc aggatgtgcc
     1921 ggaggcggcc ctgcccgagc cgatccgccg cgacaacccg tggggcgtgc ctcagcgggt
     1981 ggacggggtg ctccggtgag cgggccgctg gccggcgtcc tcgtcgccga cttctcccgg
     2041 gtcctagccg gcccgtggtg cacgatgacg ctggccgatc tgggcgccga cgtggtcaag
     2101 gtcgaacggc cgggccaggg tgacgacacc cgggcgtggg gcccaccgtg gtccgcgcac
     2161 ggcgcgac
//
LOCUS       DATAIX010000060         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_33034, whole genome shotgun sequence.
ACCESSION   DATAIX010000060 DATAIX010000000
VERSION     DATAIX010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33034"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VHM65_00985"
     CDS             complement(<1..933)
                     /locus_tag="VHM65_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704307.1"
                     /translation="MTSTRAPSLPATTRWVLFVALAVLAALACAAASSLRRVTPTTAA
                     PAQGTRVVLVGTGGLTWPDVSPGGTPSLWGLLAGGAAANLTVVAVHPTTCPVDAWVSL
                     SAGDRAGQPDLPGTSDPACTPVPLVRDGQVSGFAGLVATAHARPYDAVPGRLAARLGE
                     SGQCVGSVGPGADLAAAAPTGAVTHRAPTVAAALDGPCRVVLVDLGAVADRSGARADR
                     VGELDAAVSEVLGQVPGPVQVLVASLSGAGTPGYLGLLAARGPAAPAGLLASASTRNT
                     GLVQSSDLTATLLARAGVARPGALPGAELEVAGLA"
     gene            997..>1730
                     /gene="pheA"
                     /locus_tag="VHM65_00990"
     CDS             997..>1730
                     /gene="pheA"
                     /locus_tag="VHM65_00990"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX2704308.1"
                     /translation="MDRYGYLGPQGTFAEMALLAWPDATGAEHIPFGSVDAALAAVRS
                     GTVEAAMVPIENSVEGGVTATLDALAQGRPLVIIGEVLVPITFVLAVRPGTSLAVVRA
                     IGTHSHAWAQVRAWAGAYLPDATYVPTLSTAASAADLAQGDGDGPSYQGAVCAPVAAE
                     HYGLEVLEHDVGDNPNAVTRFVVVTRPGVIPAPTGTDKTTLVLFQKEDHAGGLLELLE
                     QFAVRGINMTRLESRPTGASMGSYCFS"
BASE COUNT          231 a          613 c          679 g          207 t
ORIGIN      
        1 cgccaggccg gccacctcga gctcggctcc ggggagggcg cccggcctgg cgaccccggc
       61 gcgagcgagc agggtcgcgg tgagatcgct cgactggacc agtccggtat tccgggtgct
      121 tgccgacgcg agcaggccgg ccggcgctgc tggtccccgg gccgcgagca ggccgaggta
      181 accgggagtg cccgccccgg acaggctcgc gacgagaacc tggaccgggc cgggaacctg
      241 gccgaggacc tcggacacgg cggcgtcgag ctcaccgacc cggtcggccc tggcgccgga
      301 ccggtcggcg acggccccga ggtcgacgag gacgacccgg cagggaccgt cgagcgctgc
      361 cgcgacggtg ggggcccggt gcgtgaccgc tccggtcggt gccgccgctg cgaggtccgc
      421 gcccgggccg accgagccca cgcactggcc ggactcgccg aggcgagcgg ccagtctccc
      481 tggcaccgcg tcatacggac gggcgtgtgc cgtggcgacg aggccggcga acccgctcac
      541 ctgaccgtcg cggaccaggg gcaccggtgt gcatgccggg tcgctggtcc ccggtaggtc
      601 gggctggccg gcgcggtctc ccgcggacag ggagacccac gcgtcgaccg ggcaggtcgt
      661 cgggtggacg gcgacgaccg tgaggttggc ggcggcgccg cctgccagca ggccccagag
      721 cgacggcgtc ccgcccggtg agacgtcagg ccaggtgagg ccaccggtac cgaccagcac
      781 gacgcgggtg ccctgggcag gggcggctgt cgtcggcgtg acccgtcgca gggagctcgc
      841 ggcggcgcag gcgagcgcgg ccagcacggc cagggcgacg aacaggaccc agcgcgtcgt
      901 ggcgggcagc gacggcgccc gggtcgaggt caccgcccca ggctaacgag tgggtcgcct
      961 agactgagcg ccgccttcag ggagaggaca acgatcgtgg accggtatgg atacctagga
     1021 ccgcagggga ccttcgccga gatggcgctg ctcgcctggc ccgatgcgac cggggccgag
     1081 cacatcccct tcgggtcggt ggatgcggcg ctggccgcag tgcgctcggg cacggtcgag
     1141 gcggcgatgg tcccgatcga gaactcggtc gagggtgggg tcacggcgac gctggacgcc
     1201 ctggcccagg gacggccgct cgtcatcatc ggcgaggtcc tcgtcccgat caccttcgtg
     1261 ctggccgtcc ggccgggcac ctccctggcg gtcgtccgag ccatcgggac ccactcgcat
     1321 gcgtgggccc aggtccgcgc gtgggccggt gcctacctgc cggacgcgac ctacgtgccg
     1381 acgctgtcga ccgctgcgtc ggcggccgac ctcgcgcagg gggatgggga cgggccgtca
     1441 taccaggggg ccgtctgtgc gccggtcgcc gccgagcact acgggctgga ggtgctggag
     1501 cacgacgtcg gcgacaaccc caacgcggtg acgcgcttcg tcgtggtgac ccggccgggc
     1561 gtcatccccg cgccgaccgg tacggacaag acgacgctcg tgctcttcca gaaggaggac
     1621 cacgccggag ggctgctcga gctgctcgag cagttcgccg tccgcggcat caacatgacc
     1681 cggctggagt cgcgcccgac cggggcgtcg atggggtcgt actgcttctc
//
LOCUS       DATAIX010000061         2243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_239751, whole genome shotgun sequence.
ACCESSION   DATAIX010000061 DATAIX010000000
VERSION     DATAIX010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239751"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2222)
                     /locus_tag="VHM65_00995"
     CDS             complement(<1..2222)
                     /locus_tag="VHM65_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2704309.1"
                     /translation="MRDVVVVEQGRLSSGTTWHAAGLVGQLRATESGTRLVQYSAALY
                     QGLEAETGLSTGYKQCGGLTVARTPERMVSLRRTAAAAEAFNLDCEILTPAQALARYP
                     ILETSDLQGAIWLPLDGTANPADVTQSLAKGARLRGARVFEKTRVLGITARDGAVTGV
                     RTDRGDIEAEIVVNCAGQWAKALAAMVGVTVPMHSAEHFYVVTDAIEGVEPMMPILRD
                     PDGYTYLKEEVGGLVVGGFEPVAKPWVAPEAIPYPFEFALLDEDWDHVEILLRSALHR
                     VPVLARTGVKKLYNGPESFTPDNQFVLGEAPEVRGFYVAAGFNSVGIASAGGAGRALA
                     EWIVSGEPQADLSAVDIRRFASFHGNTPWLHDRVGEILGLHYAVPWPNRELATARPFR
                     RSPVHHLVEAAGASFGSKMGWERPNFFAPPGMSPAIDYSWGRPSWVPWVDAEQRATRE
                     TVALYDQTSFGKLLVQGRDAEAVLQWLCTADVAVPVGRTVYTGVLNARGTYEADVTLT
                     RLAHDCFLWVTGAASPVRDHDWVVRHVGAGQHVAVTDVTGAYAVLGLMGPRSRTLLQS
                     LSRADLAHEALPFGSSREIDLGYCTVRATRITYVGELGYELYVPAEFAVSVFELLTEA
                     GAGLGLANAGHYAINALRLEKGYRAFGAELTPDYTPVEAGLLFATKLGTGIPFLGRSA
                     LVAAKAAGPRRRLVSFLLDDPDAMMWGGELLRRDGLGSGQVTSAAYATTLGACVGLAY
                     V"
BASE COUNT          340 a          780 c          821 g          302 t
ORIGIN      
        1 acataggcca ggcccacgca cgctccgagc gtcgtcgcgt acgccgccga cgtcacctgc
       61 cccgacccga gcccgtcgcg ccgcagcagc tcgccgcccc acatcatcgc gtccgggtca
      121 tcgagcagga acgacacgag ccggcgtcgg ggcccggccg ccttggcagc gacaagagcc
      181 gaacgaccca ggaacggtat gccggtgccc agcttggtcg cgaagagcag cccggcctcg
      241 accggggtgt agtcgggcgt cagctcggcg ccgaaggcac ggtagccctt ctccaggcgc
      301 agcgcgttga tcgcgtagtg gccggcgttc gcgagcccga gtccggcacc ggcttcggtg
      361 agcagctcga acacgctcac cgcgaactcg gccgggacgt agagctcgta acccagctcg
      421 ccgacatacg tgatccgggt ggcccgcacc gtgcagtagc cgaggtcgat ctcgcggctc
      481 gagccgaagg gcagcgcttc gtgcgcgagg tcggcacgcg agaggctctg gagcagcgtc
      541 cgcgaccgcg ggcccatgag gccgaggacg gcgtacgcgc cggtgacgtc ggtgaccgcg
      601 acgtgctggc cggcgccgac gtgccggacg acccagtcgt ggtcgcgcac cggagacgca
      661 gccccggtga cccagagaaa gcagtcgtgc gcgagccggg tgagcgtgac gtcggcctcg
      721 taggtgcccc gggcgttgag caccccggtg tagaccgtgc ggccgacggg caccgcgacg
      781 tcggcggtgc agagccactg caggacagcc tcggcatctc gcccctggac gaggagcttg
      841 ccgaaggacg tctggtcgta gagcgcgacc gtctcgcgag tggcacgctg ctcggcgtcg
      901 acccacggga cccagctcgg cctgccccac gagtagtcga tggcgggcga catacccggt
      961 ggggcaaaga agttgggccg ctcccacccc atcttggacc cgaagctcgc gcccgcagcc
     1021 tcgacgaggt ggtgcaccgg cgagcgccgg aagggacgcg cggtcgccag ctcgcggttg
     1081 ggccacggga cggcgtagtg caggccgagg atctccccga cccggtcgtg cagccaggga
     1141 gtgttgccgt ggaacgaggc gaagcggcgg atgtcgaccg cgctcaggtc cgcttgcggc
     1201 tccccactga cgatccactc ggcaagggcc cgcccggccc caccggccga ggcgatgccg
     1261 accgagttga agccggcggc gacgtagaag ccgcggacct cgggggcctc gccgaggacg
     1321 aactggttgt ccggagtgaa gctctccggg ccgttgtaga gcttcttgac gccggtgcgc
     1381 gccaggacgg ggacccggtg cagggcgctg cgcaggagga tctcgacgtg gtcccagtcc
     1441 tcgtcgagga gcgcgaactc gaaggggtag gggatcgcct cgggcgcgac ccacggcttg
     1501 gcgaccggtt cgaagccgcc gacgacgagg ccgccgacct cctccttgag gtaggtgtag
     1561 ccgtcggggt cgcgcaggat cggcatcatc ggctcgacgc cctcgatggc gtcggtgacg
     1621 acgtagaagt gttcggccga gtgcatcggg acggtgaccc cgaccatggc tgcgagagcc
     1681 ttggcccact gcccggcgca gttgacgacg atctccgcct cgatgtcacc gcggtcggtc
     1741 cggaccccgg tgaccgcgcc gtccctggcg gtgataccga ggacccgggt cttctcgaac
     1801 acccgggcac cgcgcagcct ggcccccttg gccaggctct gggtgacgtc ggccgggttc
     1861 gccgtgccgt cgagcgggag ccagatcgcc ccctgcagat cgctcgtctc gaggatgggg
     1921 tatcgcgcca gagcctgtgc gggggtgagg atctcgcagt cgaggttgaa cgcctcagcc
     1981 gccgcagcgg tgcgccgcag cgagaccatg cgctcaggtg tccgggccac ggtgagaccc
     2041 ccgcactgct tgtagcccgt cgacagaccc gtctcggcct ccagcccctg gtagagggcg
     2101 gccgagtact gcacgagccg cgtgccgctc tccgtggcgc gcagctggcc gacgagccct
     2161 gcggcgtgcc aggtggtgcc cgaggagagg cgcccctgct cgacgacgac gacgtcgcgc
     2221 atccccagtg cggtcaggtg gta
//
LOCUS       DATAIX010000062         3082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_92765, whole genome shotgun sequence.
ACCESSION   DATAIX010000062 DATAIX010000000
VERSION     DATAIX010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92765"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VHM65_01000"
     CDS             complement(<1..335)
                     /locus_tag="VHM65_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX2704310.1"
                     /translation="MRYLLDTHTLLWALTDPASLGDGAAQVVRDADQTLVVSAASAWE
                     IATKQRLGRLPHADAIVATYARHLRRLGAEPLPITDEHALLAGDLDWGHRDPFDRILA
                     AQAMVELLT"
     gene            complement(332..562)
                     /locus_tag="VHM65_01005"
     CDS             complement(332..562)
                     /locus_tag="VHM65_01005"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX2704311.1"
                     /translation="MTTVKVQYAKTHLSALLAAVEAGEEVVIARGDTDVARLVPVHPR
                     GERELGFVAFEVPDSFFDELPEGELAAWESGS"
     gene            complement(713..2137)
                     /gene="glnA"
                     /locus_tag="VHM65_01010"
     CDS             complement(713..2137)
                     /gene="glnA"
                     /locus_tag="VHM65_01010"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX2704312.1"
                     /translation="MFSTADEVLAFIKAQDVKFVDIRFCDLPGVMQHFNVPAQSLDED
                     FFTTGQAFDGSSIRGFQAIHESDMKLIPDVKTAYLDPFRVEKTLIMNFSIVDPFTDEP
                     YSRDPRNVAAKAEAYLKSTGIADAAYFGAEAEFYIFDDVRFETKQNASYYYIDSVEAA
                     WNTGRVEEGGNLGYKTRYKGGYFPVPPVDHFADTRDRICLNLADVGLVVERSHHEVGT
                     AGQQEINYRFASLLASGDDMMKFKYVVKNTVWATGKTATFMPKPIFGDNGSGMHTHQS
                     LWKDGEPLFYDERGYGGLSDLARWYIGGLLKHAPSLLAFTNPTVNSYHRLVPGYEAPV
                     NLVYSARNRSACVRIPIAGTSPKAKRLEFRIPDPSANPYLAFAAQLMAGLDGIKNRIE
                     PPEPVDKDLYELPPEEHAQIEQVPGSLTEVLDALEKDHDYLLAGDVFTNDLIETWVEW
                     KRVHEVDPIRFRPHPHEFEMYFDI"
     gene            2269..2736
                     /locus_tag="VHM65_01015"
     CDS             2269..2736
                     /locus_tag="VHM65_01015"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX2704313.1"
                     /translation="MLDRDDVRSWLQGPGAVRPREPGGYAGARLGLPQSGPGSVARFG
                     RRLVAVCIDWALCQLVAVGLLGVPWPWDGPQGFVPAAVFAVENLLLVGTLGYTIGHRI
                     MGIRVVSLDPRAFFPLQAWVRTMLLCLAIPALVWDRDGRGLHDKVARTVIVRG"
     gene            complement(2810..2893)
                     /locus_tag="VHM65_01020"
     tRNA            complement(2810..2893)
                     /locus_tag="VHM65_01020"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2858..2860),aa:Leu,seq:gag)
BASE COUNT          459 a          997 c         1107 g          519 t
ORIGIN      
        1 aacgtcagca gctcaaccat cgcctgcgcg gcgagtatcc ggtcgaaggg gtcgcgatga
       61 ccccagtcca ggtccccggc cagcagagcg tgctcgtcgg tgatgggcag gggctcggcg
      121 cccaggcgcc gaaggtggcg ggcataggtc gcaacgatgg cgtcggcgtg ggggagcctg
      181 ccgagccgct gcttcgtcgc gatctcccac gcgctcgcgg ccgagacgac cagtgtctgg
      241 tcggcgtccc tgaccacctg tgcggccccg tcaccgagcg aggccgggtc ggtcagggcc
      301 cagagcaggg tatgggtgtc gaggaggtac ctcacgaccc gctctcccac gcggcaagct
      361 cgccctctgg cagctcgtcg aagaagctgt cgggcacctc gaaggccacg aagcccagct
      421 ctcgttcccc acgagggtgc accggcacca gccgcgcgac gtccgtgtcg ccccgggcga
      481 tgacgacctc ctcgccggcc tcgaccgccg cgagcagggc ggacaggtgg gtcttggcgt
      541 actgcacctt caccgtggtc atgccaccag actaggctgg tcaaccatcg tggtcaaccc
      601 aacgcgaagt gccgcccgac gctgaccacc gatgagacat cgctgcaggt caggcggcac
      661 tacaccacat cctcgtcggt cagtgtcctc gctggtcagt aaggaccgcg gctcagatgt
      721 cgaagtacat ctcgaactcg tgcgggtgcg ggcggaaccg gatcgggtcg acctcgtgga
      781 cgcgcttcca ctcgacccag gtctcgatga ggtcgttggt gaagacgtca ccggcaagga
      841 ggtagtcgtg gtccttctcg agggcgtcga gcacctcggt cagcgagccg gggacctgct
      901 cgatctgggc gtgctcctcc gggggcagct cgtagaggtc cttgtcgacc ggctccgggg
      961 gctcgatgcg gttcttgatc ccgtcgaggc cggccatgag ctgggcggcg aaggcgaggt
     1021 aggggttggc cgacgggtcc gggatgcgga actcgaggcg cttggccttg ggcgaggtgc
     1081 ccgcgatcgg gatccggacg caggcggagc ggttgcgggc cgagtagacc aggttgacgg
     1141 gagcctcgta gcccgggacc aggcggtggt aggagttcac cgtcgggttc gtgaaggcga
     1201 gcagcgacgg tgcgtgcttg agcaggccac cgatgtacca gcgggcgagg tcggagagcc
     1261 cgccataccc gcgctcgtcg tagaagagcg gctccccgtc cttccacagc gactggtggg
     1321 tgtgcatgcc cgagccgttg tcaccgaaga tcggcttggg catgaaggtc gcggtcttgc
     1381 cggtcgccca cacggtgttc ttgacgacgt acttgaactt catcatgtcg tcgcccgagg
     1441 cgagcagcga cgcgaagcgg tagttgatct cctgctgccc ggcggtgccg acctcgtgat
     1501 gggagcgttc gacgacgagg cccacgtcgg ccaggttgag gcagatcctg tcgcgggtgt
     1561 cggcgaagtg gtcgaccggc gggacgggga agtagccccc cttgtaccgg gtcttgtagc
     1621 cgaggttgcc cccctcctcg acccggccgg tgttccacgc ggcctcgacg gagtcgatgt
     1681 agtagtagct cgcgttctgc ttggtctcga agcgcacgtc gtcgaagatg tagaactcgg
     1741 cctcggcgcc gaagtaggcc gcgtcggcga tccccgtcga cttgaggtag gcctcggcct
     1801 tggcagcgac gttgcgcggg tcgcggctgt agggctcgtc ggtgaacggg tcgacgatgg
     1861 agaagttcat gatgagggtc ttctcgacgc ggaacgggtc gaggtaggcg gtcttgacgt
     1921 ccgggatgag cttcatgtcc gactcgtgga tggcctggaa gccacggatc gaggacccgt
     1981 cgaaggcctg cccggtcgtg aagaagtcct cgtcgaggct ctgggcgggc acgttgaagt
     2041 gctgcatcac ccccggaaga tcacagaaac gaatgtcgac gaacttgacg tcctgggcct
     2101 tgatgaacgc gaggacctcg tcagcggtgc tgaacaactc aacctcctgg tggggggcgg
     2161 gcgcgacaac aatcccgccc gatacggtcg agccgaggct agcccggatc cgttacccaa
     2221 gggtgaccca gatgtttcgc cggtgttacg tctcgctagg ctggccgggt gcttgatcga
     2281 gacgacgtcc gctcgtggct gcaaggacct ggggcggtcc ggccgcgcga gcccggcggg
     2341 tatgccggag cgcggctcgg gctgccccag tccggccccg ggtcggtcgc ccggttcggt
     2401 cggcgcctcg tcgcggtatg catcgactgg gccctgtgcc agctggtcgc ggtcggtctg
     2461 ctgggcgtcc cgtggccgtg ggacggcccg caaggcttcg tccccgcggc ggtcttcgcc
     2521 gtggagaacc tgctcctcgt cgggacgctc ggctacacga tcggacaccg gatcatgggc
     2581 atccgggtcg tctcgctcga cccgcgcgcg ttcttcccgc tccaagcctg ggtgcggacg
     2641 atgttgctgt gcctcgcgat tccggcactc gtctgggacc gggacggccg tggcctgcac
     2701 gacaaggtgg cccggactgt catcgtccgc ggctaggccc cgcggccggg gtcgcgcccg
     2761 ccgggccgcc gcgcgagtgg cgatctgcac agacctggtc cggcttcggt gtccgagggg
     2821 ggacttgaac ccccacgccc gcaaagggca ctagcacctc aagctagcgc gtctaccatt
     2881 ccgccacccg gacgagtggt gcgtcggccg ccctggtggg gcggctcccg cgccggagac
     2941 aatagcacgc agacgcggcg ttcaccgaac cgatcgcgcc tgccgccccc cttccgtcga
     3001 cgggtgggct tatggtgccg gtatgacgcg tgagctggcc cagccggccg tcaccaccgc
     3061 cccggacgag gtcgtgcgga tc
//
LOCUS       DATAIX010000063         2253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_92776, whole genome shotgun sequence.
ACCESSION   DATAIX010000063 DATAIX010000000
VERSION     DATAIX010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92776"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VHM65_01025"
     CDS             <1..843
                     /locus_tag="VHM65_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX2704314.1"
                     /translation="GTALAPAKEGDKDIRCPNTRACPSQLRERVFGLASRGALDIEAL
                     GWKGADALLAAGVVTDEATLFALTERDLVRVPLYTRAAKKDDDPARVVDGRVLSANGD
                     RLLDNLTKAKGQPLWRVLVALSIRHVGPTAARALAGHFGSVAAITGASVADLAAVEGV
                     GPIIADAVVEWFGVDWHAGIVDQWARDGVRMADESDTSVPQTLAGLTVVVTGSMAGFT
                     RDGAKEAIVSRGGKSASSVSKNSDYVVVGPGAGSKEATARELGRPIIDEAGFVRLLET
                     GSPE"
     gene            931..1206
                     /locus_tag="VHM65_01030"
     CDS             931..1206
                     /locus_tag="VHM65_01030"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX2704315.1"
                     /translation="MRTVTVTEAKARLNELVDDAESTHEQIVITKHGHPAVVMIAADD
                     LDSLRETVFWLSKPGIRESVAEADADQSAGRTLTSEHLRRELGLPPR"
     gene            1203..1469
                     /locus_tag="VHM65_01035"
     CDS             1203..1469
                     /locus_tag="VHM65_01035"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX2704316.1"
                     /translation="MTHSVSWTGPARRDMARLPDRVATAVVVFVDERLAHAPLRLSKP
                     LSGDLADLRSARSGDYRVLFRLLEGLQVIEVVHVDHRAHAYRPG"
     gene            1535..1831
                     /gene="gatC"
                     /locus_tag="VHM65_01040"
     CDS             1535..1831
                     /gene="gatC"
                     /locus_tag="VHM65_01040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX2704317.1"
                     /translation="MSALSREDVAHLARLARIDLSDAELDRMLGELSVILDAVAQVQQ
                     APIGDVEPMSHPLPLTNVTRPDVVRPSLTAEEALAGAPQAEQQRFSVPRILDEA"
     gene            1847..>2253
                     /locus_tag="VHM65_01045"
     CDS             1847..>2253
                     /locus_tag="VHM65_01045"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX2704318.1"
                     /translation="MNSDLTRASAAELADALGRRDVSAVEATQAHLDRIRAVDGPLHA
                     FLHVDAEGALATARDVDQRRAAGEDLHHLAGVPIAVKDVMATKGLPTTCGSKILAGWI
                     PPYDATLVTRLREAGMPILGKTNMDEFAMGSST"
BASE COUNT          320 a          828 c          786 g          319 t
ORIGIN      
        1 ggcaccgccc tggctcccgc gaaggagggc gacaaggaca tccgctgtcc caatacccgg
       61 gcctgcccgt cccagctgcg ggagcgggtc ttcgggctcg cctcccgtgg agccctcgac
      121 atcgaggcgc tcgggtggaa gggcgccgac gcactgctgg cggccggggt ggtgactgac
      181 gaggcgacgc tcttcgccct caccgagcgc gacctggtcc gggtgccgct ctacacgcgg
      241 gccgccaaga aggacgacga cccggcccgg gtcgtcgacg gccgggtgct ctcggccaac
      301 ggagaccggc tcctggacaa cctcaccaag gccaagggac agccgctgtg gcgggtgctc
      361 gttgcgctgt cgatccgcca tgtcgggccg acggcggctc gggcgctggc ggggcacttc
      421 gggtcggtgg cggcgatcac gggggcatcg gtggcggacc tggcggccgt cgagggggtc
      481 ggacccatca tcgccgatgc cgtcgtggag tggttcgggg tggactggca cgccgggatc
      541 gtcgaccagt gggcccgtga cggcgtccgg atggccgacg agagcgacac gtcggtgccg
      601 cagaccctgg ccgggctcac cgtggtcgtc accgggtcga tggcgggctt cacccgggac
      661 ggcgccaagg aggcgatcgt ctcgcggggg ggcaagtcgg cgtcctcggt gtccaagaac
      721 tccgactacg tcgtcgtcgg cccgggcgcc gggtccaagg aggccacggc gcgcgagctg
      781 ggtcgcccga tcatcgacga ggccggcttc gtccgcctgc tcgagacggg ctcgccagag
      841 tgaggcgagc gccgactccc gcgccctgag ccgacgggtc tcggcttgtt ctagtccgcc
      901 acgagtctga tttcggacta tcctgaagcc gtgcgcaccg tgaccgtgac cgaggccaag
      961 gctcggctca acgagctcgt cgacgacgcc gagtcgaccc acgagcagat cgtcatcacc
     1021 aagcatggcc accccgccgt cgtgatgatt gcggccgacg acctcgactc gctccgcgag
     1081 acggtcttct ggctgtccaa gccgggcatc cgggagtccg tggccgaggc ggatgcggac
     1141 cagtcggctg ggcgcacgct cacatccgag catcttcgcc gcgagctcgg tctaccgcct
     1201 cggtgaccca ctcggtctcc tggacgggac ccgcgcgccg cgatatggcc cgactccccg
     1261 accgagttgc cacggcggtg gtcgtcttcg tggacgagcg cctggcccac gcgccgctgc
     1321 ggttgagcaa gccactgagc ggtgacctcg ccgacttgcg ctcggcgcgc agcggtgact
     1381 accgcgtgct cttccggctg ctcgaggggt tgcaggtcat cgaggtggtc cacgtcgacc
     1441 accgcgccca cgcctaccgg ccgggctagc caccggccgc ggtaggacgg gcgccacaga
     1501 tgcccgccat accgtccccc atagactggc gcgcatgtca gctctgtccc gcgaggacgt
     1561 ggcccacctg gcccggctcg cccgcatcga cctgtccgac gccgagctcg accggatgct
     1621 cggtgagctc agcgtgatcc tcgatgcggt ggcccaggtc cagcaggccc cgatcggcga
     1681 cgtcgagccg atgtcgcatc ctctgcccct caccaacgtg acgcgccccg atgtcgttcg
     1741 cccgagcctc accgcggagg aggctctcgc gggagccccc caggcagagc agcagcgctt
     1801 ctccgtgccc cgcatccttg acgaagcctg accgaggact catcccgtga actccgatct
     1861 cacccgggcc agcgctgccg agctcgccga cgccctcggc cgcagggacg tcagcgccgt
     1921 cgaggcgacc caggcccacc tcgaccggat ccgcgccgtc gacggccccc tccacgcctt
     1981 cctgcacgtc gacgccgagg gggccctggc cacggcccgc gacgtcgacc agcggcgcgc
     2041 ggccggggag gacctgcacc acctcgcggg cgtcccgatc gcggtcaagg acgtcatggc
     2101 gacgaagggc ctcccgacga cctgcggctc gaagatcctg gcgggctgga tcccgccgta
     2161 tgacgcgacc ctggtcaccc ggctgcgcga ggccgggatg cccatcctcg gcaagaccaa
     2221 catggacgag ttcgccatgg ggtcctcgac cga
//
LOCUS       DATAIX010000064         2258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_33251, whole genome shotgun sequence.
ACCESSION   DATAIX010000064 DATAIX010000000
VERSION     DATAIX010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33251"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VHM65_01050"
     CDS             complement(<1..446)
                     /locus_tag="VHM65_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704319.1"
                     /translation="MLTLAAVLAALAVLSWPAPSWAVVAREAPFAPVGDPASASRRAR
                     RRRAPGAAAGAFDADILALLGATAAGLRAGLPQADALGLALECIGSTDGRQPTVWGRR
                     GRPVSFAPVRESLRGATADGVALGPVWDAVARAAGAPQVHLVARAW"
     gene            complement(440..1624)
                     /locus_tag="VHM65_01055"
     CDS             complement(440..1624)
                     /locus_tag="VHM65_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HEX2704320.1"
                     /translation="MTVAAAGQHEVWAHIRAGRGPDTPRVAGVVAQDEVRLGRSGTAR
                     RLVELSAGLLGTGPLEPLLGDPAVTDLAVNGDGTVWVDRGDGLQPVDTLGGHEAVRAL
                     AVRLARLAGRRLDETCPYVDGQLPSGVRLHAILPPLVAGGAHICLRIPAARPIGLDEL
                     QARGTLSELVDEVLRGLVARRVSFVVTGGTGAGKTTLLAALLGASSPSDRIVVVEDAR
                     ELTVDHPHVVRLEARQPNVEGAGEVTLTTLVRQALRMRPDRLVVGEVRGAEVRELLAA
                     LNTGHDGGCGTLHANSPADVVARFEALGALAGLGSAAVHAQLLSAVQAVVHVRRDGRH
                     RFVESIGMLTRSAHGGPAVEVALRRHGRGWVRGPVWEALATVSGVSREPSSAPSRLGE
                     PC"
     gene            complement(1621..>2258)
                     /locus_tag="VHM65_01060"
     CDS             complement(1621..>2258)
                     /locus_tag="VHM65_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704321.1"
                     /translation="PDLRELSGETDGARMHASLPSVAGVAVLAGHDGPAGAPGPTGVD
                     ERGSVRDGERGSVPDAVGAEVVSSVVAGLSSVTDLVVVDLGASYPTPRGLVDTLGPLV
                     LVMGTDPHQLAAGCAAARHWAHLVDEVFLCLRAPRPDADLADMVASALDLPVLGCLAD
                     DERVRADLARGTPPGSDRRGPLGSLAQRCVEELGPGPARRSLPARQARRAS"
BASE COUNT          332 a          859 c          841 g          226 t
ORIGIN      
        1 tgccaggccc gagcgacaag gtgcacctgc ggcgccccgg cggcgcgcgc gacggcatcc
       61 cagaccgggc cgagggccac gccgtccgca gtcgcgccgc gcagcgactc gcggacgggt
      121 gcgaacgaga cgggccggcc gcgccgcccc cagaccgtcg gctgccgccc gtcggtgctg
      181 ccgatgcact ccagagccaa ccccagggca tccgcctgtg ggagccctgc tcggagcccg
      241 gcggcggtgg ctccgagcag ggcgaggatg tcggcgtcga atgcgccggc tgctgcgccc
      301 ggggcccggc ggcgacgggc ccgccgagac gctgaggctg ggtcacccac cggggcgaag
      361 ggggcctcac gcgccacgac ggcccaggaa ggggccggcc aggacagcac ggccagcgcg
      421 gccaggacgg cagccagagt cagcacggct cccccagccg ggaaggggcc gacgacggct
      481 cccgggacac tccgctgacg gtggccagcg cctcccagac gggcccccga acccacccgc
      541 ggccgtgacg gcgcagcgcg acctcgactg ccgggccacc gtgcgcagac cgggtcagca
      601 tcccgatcga ctcgacgaag cggtgcctgc cgtcgcgccg gacatgcacg acggcctgga
      661 ccgcactgag cagctgggca tgcacggcag cagagcccag cccagccaac gcaccgagcg
      721 cctcgaaccg ggccacgaca tcggctgggg agttggcgtg cagggtcccg cacccgccgt
      781 cgtgcccggt gttcagcgcg gcgaggagct cgcggacctc ggcgccgcgg acctccccga
      841 cgacgagccg gtctggtcgc atccgcagtg cctggcgcac cagggtggtc aaggtgacct
      901 ccccggcgcc ctcgacgttg ggttgtcgag cctcgagccg cacgacgtgg gggtggtcga
      961 cggtgagctc acgcgcatcc tcgacgacga cgatccggtc cgagggggag ctggccccca
     1021 gcaaggccgc gagcagcgtc gtcttgccgg cgccggtccc gcccgtgacg acgaacgaga
     1081 ctcgtcgtgc gaccaggccg cggagtacct cgtccacgag ctcggacagg gttccgcggg
     1141 cctggagctc gtcgaggccg attgggcgcg cggcggggat gcgcaggcag atgtgcgccc
     1201 cgcccgccac gagcggaggc aagatggcgt gcaggcgaac gccggacggc agctggccgt
     1261 cgacatacgg gcaggtctcg tcgagccggc ggccggcgag ccgggcgagg cgaacggcca
     1321 gggcgcgcac ggcctcgtgg ccgccgaggg tgtcgaccgg ctgcagacca tccccgcggt
     1381 cgacccatac cgtgccgtcg ccgttgaccg cgaggtcggt gaccgcggga tcgcccagga
     1441 gcggctcgag ggggccggtc ccgagaagtc ccgcagacag ctccaccagg cggcgggcgg
     1501 tccctgacct cccgagacgc acctcgtcct gagcgacgac gccggccacc cgcggggtgt
     1561 ccggcccccg cccggccctg atgtgagccc agacctcgtg ctgtcccgcc gcggcgaccg
     1621 tcatgatgcc cgccgggcct gccgcgccgg caacgagcgc cgcgccggcc cggggccgag
     1681 ctcctcgacg caccgctgag cgagggagcc cagcggcccc cgccgatcgc ttcccggcgg
     1741 tgttcccctg gccaggtcgg cgcgcacccg ctcgtcgtcg gccagacatc cgaggacggg
     1801 caggtcgagg gcggatgcca ccatgtcggc gaggtcggcg tctggccgtg gggcgcgaag
     1861 gcacaggaag acctcgtcga ccaggtgagc ccagtgccgg gcggccgcgc acccggcagc
     1921 gagctggtgc gggtcggtgc ccatgaccag gacgagcgga cccagcgtgt ccacaagccc
     1981 tcggggggtt gggtatgacg cacccaggtc cacgacgacg agatcggtga ccgacgagag
     2041 tcccgcgacg accgaggaca cgacctcagc gccgaccgca tccggcaccg agccccgctc
     2101 gccatcccgc accgagcccc gctcgtcgac cccggtcgga ccaggggccc cggccggccc
     2161 atcatggccc gcaagcacgg cgacacccgc gacgctcggc agggaggcat gcatgcgagc
     2221 gccgtcggtc tcaccggaca gctcacgcag gtcaggcc
//
LOCUS       DATAIX010000065         1807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_33350, whole genome shotgun sequence.
ACCESSION   DATAIX010000065 DATAIX010000000
VERSION     DATAIX010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33350"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1179)
                     /locus_tag="VHM65_01065"
     CDS             complement(172..1179)
                     /locus_tag="VHM65_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2704322.1"
                     /translation="MDLVVLGGTAYLGRYVTQAALARGHRVTCLARGTSGQPPEGAVL
                     VPVDRDQDGALSPVSAKHWDSVIDVARHPGHVRRAVSALAGAAGHYVFVSTGNVYADH
                     RRPGLDESAPTLPPLDGEVMTDMSEYGAAKVACENHVLAGFGPGRSLIARSGLIGGPD
                     DRSGRSGYWPWRFAHPSNPEGRVLVPDTPELRTQVIDVRDLAQWLVRCAERRTAGVYD
                     AVGHTVRMHDHLALAREVARHTGPLVPVSSAWLAAHEVAEFMGPRSLPLWLHDPGWLG
                     FTHRRGEAARTAGLHSRPLRETLEAALASESERPTGTPRGAGLTDDEERDLLARVPAD
                     G"
     gene            1415..>1807
                     /locus_tag="VHM65_01070"
     CDS             1415..>1807
                     /locus_tag="VHM65_01070"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2704323.1"
                     /translation="MRGRSESDGTVDRWSRHDWWGVVLEAPDARALAHFYADLLGWAV
                     ASQSDTDAAIGPADGVAYLAFQTTSSYVRPVWPAVPGTQQITMHLDFEVDDLEPAVAH
                     AVSLGGELAAFQPQDNVRVVLDPAGHPFC"
BASE COUNT          235 a          648 c          676 g          248 t
ORIGIN      
        1 ggcctgggcc ctggcccgca cgggcgacgg cgctgcgccg gccccggcct gggccgtgag
       61 cccggcaggc gagggcggcc ttcccgccga ttccgcgggg gtcggcgcgc aggtgcgcga
      121 ccgataccga gggctgcgcg agaccgtcca cccacgctga ctgtcccgag ctcaaccgtc
      181 agccgggacc cgagccagga ggtcgcgctc ctcgtcgtcg gtcaggcccg ccccacgagg
      241 cgtgccggtg ggtcgttcgg actcggacgc cagtgccgcc tcaagggtct cgcgcagcgg
      301 ccgggagtgc agacccgccg ttcgtgccgc ctcgccgcga cggtgcgtga agccgagcca
      361 gccggggtcg tgcagccaga gcgggagaga ccgtggcccc atgaactccg cgacctcgtg
      421 ggcggccagc cacgcggacg acacgggaac gagcggcccg gtatggcggg ccacctcgcg
      481 agcgagggcg aggtggtcgt gcatcctcac ggtgtggccg acggcgtcgt acacacccgc
      541 ggtgcggcgc tcggcgcacc ggacgagcca ctgcgccagg tcccggacgt cgatgacctg
      601 ggtgcgcagc tcaggggtgt cgggcacgag cacgcggcct tcggggttgc tcggatgggc
      661 aaaccgccac ggccagtagc cgcttcgtcc gctccggtca tccgggccgc cgatgagccc
      721 cgatctcgcg atgagcgagc gccccggccc gaagcccgcc aggacgtggt tctcgcaggc
      781 gaccttggcg gcgccatact cgctcatgtc ggtcatgacc tccccgtcca gcggtggcag
      841 ggtcggagcg ctctcgtcca ggccgggtct gcggtggtcg gcgtagacgt tgcccgtcga
      901 gacgaacacg tagtgcccgg cggcgccggc gagcgcactc acggcgcgac ggacgtgtcc
      961 tgggtggcgg gccacgtcga tgaccgagtc ccagtgcttg gcactgaccg gtgacagggc
     1021 gccgtcctgg tcgcggtcca ccggcacgag aaccgcgccc tcggggggct gtccgctcgt
     1081 gccgcgagcc aggcaggtga cgcggtgccc gcgggccagc gcagcctggg tgacatagcg
     1141 gccgaggtac gccgtcccgc cgagtaccac gaggtccatg gcgccagggc agcacggcgc
     1201 cgctcgtggc gacaaggggt tttcgccctg ggcgcagagc agctccgcgg ccggcaggtg
     1261 tgtcgcagcc gtccccgggc cgagcatgat ggggccatgg cgcgtgagga tcacggttgt
     1321 tgccctcgca ccggcgctgg ctccgccgaa gctcggtcta cgccccaagg tagctcgcct
     1381 tgggtgccga ccggtgctct ggcacagtag tgggatgcgc gggcgctcgg agtcggacgg
     1441 gacggtcgac cggtggtcgc gccacgactg gtggggagtc gtgctcgaag cacccgatgc
     1501 gcgggcgctc gcacacttct acgcggacct gctcggctgg gcggtcgcct cgcagagtga
     1561 tacggatgcc gcgatcgggc ccgccgacgg ggtcgcctac ctcgccttcc agacgacatc
     1621 gtcctacgtc cggccggtgt ggccggcggt gcccggcacc cagcagatca cgatgcacct
     1681 cgacttcgaa gtcgacgacc tggagcccgc ggtggcccac gccgtctcgc tcggcggtga
     1741 gctcgccgct ttccagccgc aggacaacgt ccgcgtggtc ctggaccccg ccgggcaccc
     1801 cttctgc
//
LOCUS       DATAIX010000066         1863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_239891, whole genome shotgun sequence.
ACCESSION   DATAIX010000066 DATAIX010000000
VERSION     DATAIX010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239891"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..801
                     /locus_tag="VHM65_01075"
     CDS             112..801
                     /locus_tag="VHM65_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2704324.1"
                     /translation="MTPGRPGPVRAWWDQWAMPRLVDVVLSDATVGVWRQRLCADVAG
                     DVLEVGFGSGRNLPFYGPDVSGVLAVEPSGPAWVRARQRVTTFARPVERIGLDGARIP
                     LPDASVDAVVSTWTMCSIADLTSALSEMRRVLRPGGVIHFVEHSLAPDPGVARVQRAL
                     QPAWGQVSGGCHLDRDIPAELAAAGLTTAAVQTGYVTWLALAKPFGWFCLGRATAGPD
                     HPSAAGAVEAT"
     gene            complement(849..>1863)
                     /locus_tag="VHM65_01080"
     CDS             complement(849..>1863)
                     /locus_tag="VHM65_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2704325.1"
                     /translation="DEADPPAGGDLLASAFPVDADRRIARVNGVVTGGVFWSVPEKET
                     AASGTRPWPSGPDAQAVTVVAATPGMLRAAKLHLTAGTTFDEFHDRRAEPVTVVSHSV
                     AERLHLKPLETHPVIFVGGTALYVIGVYDGVVRQPDLLLSIVVPAGFASQVWGAPTSE
                     NPAAMVVETALGAAQQVASQAAVALRPQSPERLRATAPPDPRDLREQVNADVSALLVV
                     LAGLSLVVGALGIANSTLVAVMERVGEIGLRRAVGARPRHIAGQFLVESTALGTIGGL
                     IGTALGSVGVVLLSLVRGWTPLMEPGLVIVAPFIGTLVGLLAGMYPAWRAARIEPVEA
                     LRR"
BASE COUNT          257 a          700 c          665 g          241 t
ORIGIN      
        1 gtggcagagt gggcggatgc gaagcagtga ccgagtggaa ccagagaccc agtggaacca
       61 gagacccacg ggggccagtg accgaatgga cccagagacc cagtggagcc agtgaccccg
      121 gggcggcccg ggccggtgcg cgcctggtgg gaccagtggg ccatgccccg gctcgtcgac
      181 gtggtcctgt ccgacgcaac ggtcggcgta tggcgccagc ggctgtgcgc cgacgtcgcc
      241 ggcgacgtgc tcgaggtggg gttcgggtcg ggacggaacc tgccgttcta cggccccgac
      301 gtctctggag tcctcgcggt cgaaccctcc ggcccagcgt gggtgcgggc ccgtcagcgg
      361 gtcaccacct tcgcccgtcc ggtcgagcgg atcggcctgg acggggcccg catcccgcta
      421 cccgatgcca gcgtcgacgc cgtcgtgtcg acgtggacga tgtgcagcat tgccgacctc
      481 acgtcggccc tttccgagat gcgccgggtc ctgcgtccgg gcggtgtcat tcacttcgtc
      541 gagcactcac tcgcgcccga tcccggcgtg gcccgagtgc agcgcgccct ccagcccgcc
      601 tggggtcagg tgtccggagg ctgccatctg gaccgcgaca tccctgcgga gctggcggcc
      661 gcgggactca ccacggcggc tgtccagacg gggtacgtca cgtggcttgc gttggccaag
      721 cccttcgggt ggttctgcct gggccgggcg acggccgggc cggaccaccc gtcagcggcg
      781 ggcgcggtcg aggcgacctg acgctcgccg ggcctggccg gtcgcccggt cgcgcccggt
      841 cgcactcctc agcggcgcag cgcttcgacg ggctcgatcc gcgccgcgcg ccacgcgggg
      901 tacatcccgg cgagcagccc cacgagcgtg ccgatgaacg gcgccacgat caccagcccc
      961 ggttccatca acggggtcca cccgcgcacg agcgagagca ggacgacccc caccgaaccg
     1021 agcgcggtcc cgatcagccc gccgatggtc cccagggccg tcgactcgac gaggaactgc
     1081 ccggcaatgt gccggggcct cgcgccgacc gcccgtcgca gcccgatctc cccgacccgc
     1141 tccatcacgg ccacgagcgt ggagttggcg atccccagtg cgcccacgac gagagacagc
     1201 ccggccagga ccacgagcag ggcgctgaca tcggcattca cctgctcgcg caggtcgcgc
     1261 gggtccggcg gggccgtggc ccgcagccgc tccggggact gcggccgcag cgccacggcc
     1321 gcctggctgg ccacctgctg ggccgccccg agcgcggtct cgacgaccat cgccgccggg
     1381 ttctcgctcg tcggcgctcc ccagacctgg gacgcgaagc cggccggcac gacgatcgag
     1441 agcaggaggt ccggctggcg gaccaccccg tcatagacgc cgatgacgta gagggcggtg
     1501 ccgccgacga agatcaccgg gtgggtctcc agcggtttga gatgcagccg ttcggcaacc
     1561 gagtggctca ccaccgtgac cggttcggct cggcggtcgt ggaactcgtc gaacgtcgtg
     1621 cccgccgtca ggtgcagctt cgccgcgcgc agcatccccg gcgtcgccgc cacgaccgtg
     1681 accgcctgcg cgtcgggccc ggacggccac ggccgggtgc cggacgctgc cgtctccttc
     1741 tccggcaccg accagaagac accgccggtc accacgccgt tgacccgggc gatccggcgg
     1801 tccgcatcga cggggaacgc cgacgccagc aggtcaccac cggccggcgg atcggcttcg
     1861 tct
//
LOCUS       DATAIX010000067         1752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_33410, whole genome shotgun sequence.
ACCESSION   DATAIX010000067 DATAIX010000000
VERSION     DATAIX010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33410"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VHM65_01085"
     CDS             complement(<1..211)
                     /locus_tag="VHM65_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704326.1"
                     /translation="MGRARRARQIAQTAAYGGGVGMAGLGAVGAVGYGLIRLEAVLAR
                     RVVEGILGTPPVDRGTYGTGSGRPLE"
     gene            310..>1752
                     /locus_tag="VHM65_01090"
     CDS             310..>1752
                     /locus_tag="VHM65_01090"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR01137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HEX2704327.1"
                     /translation="MLAVARRSSGGVCEDDAVKYAEHIADLVGHTPLVKLSSVTNGLA
                     ATILAKVEYLNPGGSVKDRIALRMIEAAEASGELRPGGTIVEPTSGNTGVGLALVAQR
                     RGYACVFVCPDKVSEDKRTVLKAYGAQVVVCPTSVPPEHPDSYYSVSDRLVRETPGAW
                     KPNQYANPEGPNSHYATTGPEIWADTDGRVTHFVAGVGTGGTITGTARYLLEVSADRP
                     AGRVQVIGADPEGSVYSGGTGRPYLVEGVGEDFWPAAYDPSVVDEVIAVSDADSFAMT
                     RRLAREEGLLVGGSCGMAVVAALRAARDLPAGAVAEAVVVVILPDSGRGYLSKIFNDE
                     WMASYGFLRQDSGRTVGEVLRSKSGALPALVHTHPTETVRDAIEILREYAVSQMPVVK
                     AEPPVTTGEVAGAVSERHLLELLFTGQAHLADPVERHMEAGLPLVAATGSPAPTSGSP
                     ASMWRSTGSARWACPVNSSSSRCRSLTAPAT"
BASE COUNT          248 a          621 c          621 g          262 t
ORIGIN      
        1 tctccaaagg ccggcccgag ccggtcccat aggtgccgcg gtccacgggc ggggtgccga
       61 ggatgccttc gacgacccgc cgggcgagga ccgcctcaag gcggatgagg ccatagccca
      121 cggcgccgac ggcgccgagt cccgccatgc cgactccacc gccatacgcg gcggtctggg
      181 cgatctggcg tgcacgtcgg gcgcgtccca tgggctgctc atccttccgg tccctagaga
      241 agacgtcccc acggtaggtg tgggttcccc tgtatctgag gggattgggc gaaccgagat
      301 acagccgaga tgctcgcggt cgcccgacgg tcttccggcg gtgtctgcga ggatgacgcg
      361 gtgaagtacg ccgagcacat cgccgacctc gtcgggcaca cccccctcgt caagctctcc
      421 tcggttacca acggactggc cgccacgatc ctagccaagg tcgagtacct caaccccggc
      481 gggtcggtca aggaccgcat cgcgctgcgg atgatcgagg cggccgaggc ctccggcgag
      541 ctgcgtccgg gcggcacgat cgtcgagccc acctcgggca acaccggggt cggactggcg
      601 ctcgtggccc agcggcgcgg ttacgcgtgt gtgttcgtct gccccgacaa ggtctccgag
      661 gacaagcgca ccgtgctcaa ggcgtatggc gcgcaggtcg tcgtgtgccc cacatccgtc
      721 cctcctgaac accctgactc ctactactcg gtctcggacc ggctcgtccg ggagaccccg
      781 ggggcatgga agccgaacca gtacgccaac cccgagggac ccaacagcca ctacgccacg
      841 acgggccctg agatctgggc cgacacggac gggcgggtca cgcacttcgt cgccggtgtc
      901 gggacgggtg gcacgatcac cgggaccgcg cgctatctgc tcgaggtgtc ggcggaccgg
      961 cccgccggcc gggttcaggt catcggtgcc gacccggagg ggtcggtcta ctccggcggc
     1021 accgggcgcc cctacctcgt cgagggcgtc ggcgaggact tctggccggc tgcgtatgac
     1081 ccgtcggtgg tcgacgaggt catcgccgtc tcggacgccg actcgttcgc catgacccgg
     1141 cgactggccc gtgaggaggg tctgctcgtc gggggttcct gcggtatggc ggtcgtcgcg
     1201 gctctgcgtg cggcgcggga cctgcccgcc ggggcggttg ccgaggcggt tgtcgtcgtg
     1261 atcctgcccg actcggggcg cggctacctg tccaagatct tcaacgacga gtggatggcc
     1321 tcatacggct tccttcgtca ggacagcggc cgcacggtgg gggaggtcct gcgctccaag
     1381 agcggggcgc tcccggcgct ggtgcacacc cacccgaccg agaccgtgcg cgacgccatc
     1441 gagatcctgc gcgagtacgc cgtctcgcag atgccggtcg tcaaggccga gccaccggtg
     1501 acgacgggtg aggtcgccgg cgcggtgagc gagcggcacc tgctcgagct gctattcacc
     1561 gggcaggccc atctcgccga cccggtcgag cgccacatgg aggcggggct gccgctcgtg
     1621 gccgccactg gctccccggc gcccacgagc ggcagccccg cctccatgtg gcgctcgacc
     1681 gggtcggcga gatgggcctg cccggtgaat agcagctcga gcaggtgccg ctcgctcacc
     1741 gcgccggcga cc
//
LOCUS       DATAIX010000068         2486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_33475, whole genome shotgun sequence.
ACCESSION   DATAIX010000068 DATAIX010000000
VERSION     DATAIX010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33475"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1236
                     /gene="ygeW"
                     /locus_tag="VHM65_01095"
     CDS             46..1236
                     /gene="ygeW"
                     /locus_tag="VHM65_01095"
                     /inference="COORDINATES: protein motif:HMM:NF005538.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HEX2704328.1"
                     /translation="MDLEEQLRTLGGLDCAGLYQDDFLLTWDKSEDEIRAVLAVADAL
                     RALRENNKSARIFDSGLAISLFRDKSTRTRFSYASAGNLLGLEVQDFDEGKSQVAHGE
                     TVRETANMISFMADVVGIRDDMYIGKGHTYMTEFAHYVHEGHAAGVLEQRPTLVSLQC
                     DIDHPTQSTADLLHLVHEFGGLENLRGKKLAMTWAYSPSYGKPLSVPQGMIALMTRFG
                     MDVTLAHPQGYDVMAQTREIAATNAGQSGGRFSVTDSMEEAFEGADIVCPKSWAPYAA
                     MQERTDLYGTGDTDGIKDLEQGLLAQNAEHRDWTTTEAMMAKTKDGTALYLHPLPADI
                     TGVSCVQGEVDASIFDRYRDPLYKQASNKPYAIAAMIFLQKVKDPAAKLAQLWDREQP
                     RWQS"
     gene            1292..>2486
                     /locus_tag="VHM65_01100"
     CDS             1292..>2486
                     /locus_tag="VHM65_01100"
                     /inference="COORDINATES: protein motif:HMM:NF009555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HEX2704329.1"
                     /translation="MSTAVQIDYDAVKAAAEAHRGDMSRFLRALIKAKGTSCQEEEKA
                     RLIQAEMTTLGYTKTEIDPLGNILGYMGTGPTLIGFDGHIDTVGVGNPDNWTFDPFEG
                     YEDDVNIGGRGASDQLGGVVSAVYGAAIAKELGLLSDKYTMLVTGTVQEEDCDGMNWL
                     YIIEESGIRPAFVVSTEPTDNGVYRGHRGRMEIRVEVSGVSCHGSAPERGDNAIYKMA
                     EILLEVRDLNDTLLHNDFLGKGTVTTSQIFFTSPSRCAVADSCAISLDRRLTDGETWQ
                     SAIKEIEALPSVQKWGAKVSLYDYKATAWTGLEYGQECYFPTWVVAEDSPEIQAAAKA
                     HEAMYGEPRIDKWTFSTNGVAIAGRHGIPVIGVGPWQEAQAHAPNEVNWKDDLVKCAA
                     MYSVIP"
BASE COUNT          488 a          845 c          812 g          341 t
ORIGIN      
        1 tagatggatg ccaccagagg acgacacgag gagcgaagga gccggatgga cctcgaggag
       61 cagctacgca cgctgggtgg ccttgactgt gcaggcctgt accaggacga cttcctcctg
      121 acgtgggaca agtccgagga cgagatccgc gcggtgctcg ccgtcgcgga cgcgctgcgg
      181 gccctgcggg agaacaacaa gagcgcacgg atcttcgact ccgggctggc catctccttg
      241 ttccgggaca agtcgacccg cacgaggttc tcctacgcct cggcgggcaa cctgctcggg
      301 cttgaggtcc aggacttcga cgagggcaag tcgcaggtgg cgcacggcga gacggtgcgc
      361 gagacggcga acatgatctc gttcatggcc gacgtcgtcg gcatccgcga cgacatgtac
      421 atcggcaagg gacacaccta tatgacggag ttcgcccact acgtccacga ggggcacgca
      481 gcgggtgtcc tcgaacagcg gccgaccctg gtgagcctgc agtgcgacat cgaccacccc
      541 acgcagtcca ccgccgacct gctccacctc gtccacgagt tcggcgggct ggagaacctg
      601 cgcggcaaga agctcgcgat gacctgggcc tactcgccga gctacggcaa accgctgtcc
      661 gtcccgcaag ggatgatcgc cctgatgacc cggttcggca tggacgtcac cctggcccac
      721 ccgcaggggt atgacgtcat ggcacagacc cgcgagatcg ccgcaacgaa cgccgggcag
      781 tccggcggcc gcttctccgt gacggactcg atggaggagg cgttcgaggg cgccgacatc
      841 gtgtgcccga agagctgggc gccatacgcg gcgatgcagg agcgcaccga cctctacgga
      901 accggcgaca ccgacggaat caaggacctc gagcagggac tgctcgcgca gaacgccgag
      961 caccgggact ggaccaccac cgaggcgatg atggccaaga ccaaggacgg cacggcgctc
     1021 tacctgcacc cgctgccggc cgacatcacc ggcgtgagct gcgtccaggg cgaggtcgac
     1081 gcgtcgatct tcgaccgcta ccgcgacccg ctctacaagc aggccagcaa caagccctac
     1141 gccatcgcgg cgatgatctt cctgcagaag gtcaaggacc ccgcggccaa gctcgcgcag
     1201 ctctgggacc gcgagcaacc gcgctggcag tcctgaaccc ctccggcgtc ctgcaacggt
     1261 cgtccatgca ccagagaacc aaggagacac aatgagcacg gcagtccaga tcgactacga
     1321 cgcggtcaag gcggcggcag aggcccaccg cggcgacatg tcccggttcc ttcgcgccct
     1381 gatcaaggcc aagggcacga gctgccagga ggaggagaag gccaggctca tccaggcgga
     1441 gatgaccacg ctcggctata ccaagaccga gatcgacccc ctgggcaaca tcctcggtta
     1501 catgggcacc ggcccgaccc tcatcggttt cgacgggcac atcgacaccg tcggcgtggg
     1561 taaccccgac aactggacct tcgacccgtt cgagggctac gaggacgacg tcaacatcgg
     1621 cggccgcggc gcctccgacc agctcggcgg ggttgtctcc gcggtctacg gggcggcgat
     1681 cgccaaggag ctcgggctgc tgtccgacaa gtacacgatg ctcgtgaccg gcaccgtcca
     1741 ggaggaggac tgcgacggca tgaactggct ctacatcatc gaggagtccg gcatccgtcc
     1801 ggcgttcgtc gtgtcgaccg agcccaccga caacggcgtc taccgcggcc accgcggccg
     1861 gatggagatc cgtgtcgagg tgagcggcgt ctcctgccac ggttcggcac ccgagcgtgg
     1921 cgacaatgcg atctacaaga tggccgagat cctcctcgag gtgcgcgacc tcaacgacac
     1981 gctgctgcac aacgacttcc tcggcaaggg gacggtcacc acgtcgcaga tcttcttcac
     2041 ctccccgtcc cgatgcgcgg tcgccgacag ctgcgcgatc tccctcgacc ggcggctcac
     2101 cgacggcgag acgtggcaga gcgcgatcaa ggagatcgag gcactgccct cggtccagaa
     2161 gtggggcgcg aaggtctcgc tctacgacta caaggccacc gcgtggaccg ggctggagta
     2221 cggccaggaa tgctacttcc cgacctgggt cgtcgccgag gactcgccgg agatccaggc
     2281 cgcggcgaag gcgcacgagg cgatgtatgg cgagccccgc atcgacaagt ggaccttctc
     2341 gaccaacggg gtcgccatcg caggccggca cggtatcccc gtcatcgggg tcggcccctg
     2401 gcaggaggcc caggcgcacg caccgaacga ggtcaactgg aaggacgacc tcgtcaagtg
     2461 cgcggcgatg tacagcgtca tcccga
//
LOCUS       DATAIX010000069         1922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_3380, whole genome shotgun sequence.
ACCESSION   DATAIX010000069 DATAIX010000000
VERSION     DATAIX010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3380"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(402..473)
                     /locus_tag="VHM65_01105"
     tRNA            complement(402..473)
                     /locus_tag="VHM65_01105"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(438..440),aa:Arg,seq:ccg)
     gene            545..1000
                     /locus_tag="VHM65_01110"
     CDS             545..1000
                     /locus_tag="VHM65_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2704330.1"
                     /translation="MEWSLAEGGARLGEAGSVFVCDDTPSVRHLIRINLELEGYAVRE
                     ADDGAVLVGELDRLIQEGRTLPQVVIIDSQMHPNDGWWAIDQIRSTPGLVDLPVIMVT
                     ASLQQHDRVQARAAGLDAFVGKPFDPDHLVALVGGFCQHGRAFASRDRP"
     gene            1144..>1922
                     /locus_tag="VHM65_01115"
     CDS             1144..>1922
                     /locus_tag="VHM65_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704331.1"
                     /translation="MHAHEAGALHADGYRGPTWLQRPDDASALIPSLWATSVGRSADG
                     VLSVGGMSVTHLASAFGTPAYVLDEADFRSRAREFVTSYTEMFDDLCGGADVYYAGKA
                     FLCAEVARWVREDGLHLDVCSGGELAVAQRAGIPGERIGMHGNNKSLAEIEAGLEYGV
                     GRFIVDSFEEIERLALAAQRLGQIAPVLIRVTVGVEAHTHEFIATAHEDQKFGFSLSG
                     GEAAEAVRRVLGHTPNLALLGLHSHIGSQIFDTSGFEISARR"
BASE COUNT          265 a          688 c          660 g          309 t
ORIGIN      
        1 tcgcggcgag tgggaccctg aggcgctgct gttcaccggt gatcttgatg gcgcccggac
       61 cgcggcgtgg ccgtgccgca ccccgggttg cctcaccgcg acccgcttcc atcaccggcg
      121 ctgcgacggc tgccgacggg caccagacgt gcctggtgcg gcagcggggt ggccggtgct
      181 gtgcctaccg ccgtccaccg ggacgtgcgc ggcgccactc cgagcgcttc gagctcggcg
      241 cgggcgggcg aaggcgggtt gatgatcggc catcaagggg tcgcctggcg ttgctcgtcg
      301 cgtgttacgg agccttgcag tgatgcgctg gccaattgca cgtttgttgc acgcgacgac
      361 cgacagatta cgtttgccca ggtcagggcc ctgcccgtta gtgcccccga caggattcga
      421 acctgcgcaa ccgcctccgg agggcggtgc tctatcccct gagctacggg ggccttgcgg
      481 cccggcaccc ggcgttcgca ccgactgcct gcgcccggaa ggctagcagc ctaggctgcc
      541 attcatggaa tggagcctcg cggagggggg cgctcggctc ggcgaggccg gcagcgtctt
      601 cgtctgcgac gacacgccct cggtgcgcca cctcatccgg atcaacctcg agctcgaggg
      661 gtatgccgtg cgcgaggccg atgatggcgc ggttctggtc ggcgagctcg accggctcat
      721 ccaggagggc cggaccctgc ctcaggtcgt catcatcgac tcgcagatgc acccgaacga
      781 cgggtggtgg gccatcgacc agatccgcag caccccaggc ctggtcgacc tccccgtcat
      841 catggtgacg gcgtccctgc agcagcacga ccgggtccag gcccgggcgg ccggcctgga
      901 cgccttcgtc ggcaagccgt tcgacccgga ccatctcgtc gctctggtag gcgggttctg
      961 ccagcacgga cgggcattcg cgagtcggga ccggccgtga gatcggccga ccccgctccg
     1021 cgcccggcgc gtcgccgacc gcggagcatg agacgcgctg cgttgtggcc gaggcggctc
     1081 gtatcgtcat ctcgtcgagc ctgacctcgc tcgccgtcac cgcccccaga acggatctag
     1141 cccatgcatg ctcacgaggc cggcgctctt catgccgacg ggtaccgcgg tcccacctgg
     1201 ctgcagcggc cagacgacgc ctccgccctc atcccctcgc tctgggccac cagcgtcgga
     1261 cggtccgccg acggggtgct ctcggtcggt ggcatgtcgg tgacccacct cgccagtgcg
     1321 ttcgggaccc cggcgtacgt cctcgacgag gccgacttcc gttcccgcgc cagggagttc
     1381 gtgacgtcat acaccgagat gttcgacgac ctgtgcgggg gagcggacgt ctactacgcc
     1441 ggcaaggcct tcctgtgtgc cgaggttgcc cgatgggtcc gagaggacgg gcttcacctc
     1501 gacgtctgct cgggcggcga gctcgcggtg gcgcagcggg ccgggattcc cggcgagcgc
     1561 atcggcatgc acggaaacaa caagagcctc gccgagatcg aggcggggct ggagtatggc
     1621 gtcggccgct tcatcgtcga ctcgttcgag gagatcgagc ggctggccct cgccgcgcag
     1681 cggctcggac agatcgctcc cgtgctcatc cgcgtcaccg tcggcgtcga ggcccacacc
     1741 cacgagttca tcgccaccgc ccacgaggac cagaagttcg gcttctccct gagcggcggc
     1801 gaggcggccg aggcggtgcg ccgcgtcctc ggccacacgc ccaacctggc gctgctcggt
     1861 ctgcacagcc acatcggttc gcagatcttc gacacctccg gcttcgagat ctcggcccga
     1921 cg
//
LOCUS       DATAIX010000070         3866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_93033, whole genome shotgun sequence.
ACCESSION   DATAIX010000070 DATAIX010000000
VERSION     DATAIX010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93033"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1346
                     /locus_tag="VHM65_01120"
     CDS             <1..1346
                     /locus_tag="VHM65_01120"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX2704332.1"
                     /translation="SEMPTRWYNLVHDLPTPPPPYLHPGTGLPVTPDDLTPLFPMALI
                     EQEVTTEQYVEIPGEVLDVYRLWRPSPLYRAHRLEKALGTPARLYYKYEGVSPAGSHK
                     PNTSVPQAFYNSQAGIRKLTTETGAGQWGTALAFACAQFDLECEVWQVRASYDSKPYR
                     RMMIETFGGTVHPSPSSLTAAGRAILAKDPGSPGSLGIAISEAVEVAAQSPDTNYALG
                     SVLGHVLLHQTIIGEEALLQLAKIGESVDLIVGCTGGGSNFGGLTFPFLREKLAGRMS
                     PVIRAVEPASCPSLTQGTYAYDFGDTAGMTPLMKMHTLGHDFIPDPIHAGGLRYHGMS
                     PLISHLVEEGFIEAIAKTQQDCFAAGVQFARTEGILPAPEPTHALAAAIDEALRCKET
                     GEEKVILTALCGHGHLDLPAYDAYLSGTLVDHTWADAELKASVAEALTHLPAIPG"
     gene            complement(1349..2947)
                     /locus_tag="VHM65_01125"
     CDS             complement(1349..2947)
                     /locus_tag="VHM65_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX2704333.1"
                     /translation="MIAATAVELRAGSRLLLDGATFRINDGDRVGLVGRNGAGKTTLT
                     RVLAGEGPPAAGSVVVSGTVGYLAQDPRTGDLDARARDRILAARGLDVIVHRLREAEK
                     GMASLDEAVRDKAMARYARLDAEFQAAGGYGAESEAAAIASALGLDERVLGQPLGTLS
                     GGQRRRVELSRILFSSASTLLLDEPTNHLDADSVVWLRDHLKAHKGGLVVISHDVALL
                     DQVVTRVFHLDANRAELDVYNVGWKAYLLARETDERRRRRERLNAEKKASVLLAQADR
                     MRAKATKAVAAQNMARRAERLLASIEGERVHDRVAKLRFPSPARCGKTPLTATGLSRS
                     YGSLEVFTDVDLAIDRGSRVVVLGLNGAGKTTLLRILGGIDPPDTGVVEAGHGLKLGY
                     YAQEHENLDVSRSVLENMKTAAPDLGETEVRNVLGSFLFTGDDVDKPAGVLSGGEKTR
                     LSLATLVVSAANVLLLDEPTNNLDPASRAEILGALRTYKGAVVLVTHDEGAVQALEPE
                     RILLLPDGVEDLWGPDYLDLIALA"
     gene            3151..>3866
                     /locus_tag="VHM65_01130"
     CDS             3151..>3866
                     /locus_tag="VHM65_01130"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX2704334.1"
                     /translation="MSFTENVQLDTSRIESGGGGRGLAGIKVGGGIGGVIVMVVVVLF
                     QVLGNGGGGSGGTLPIDAAQYDASRVAAGGQVDPAAFSQCRTGADANRDDVCRIIATV
                     NSAERFWAEELPRRGLRFRPAQTVIYDGTTQSACGTASNQVGPFYCPLDEKIYIDASF
                     FAELTSRFGADDGALAKEYVVAHEYGHHIQNILDILGRAQQDPQGPESGAVRIELMAD
                     CLGGMWVKNATTTTDAQGVT"
BASE COUNT          588 a         1441 c         1308 g          529 t
ORIGIN      
        1 agagcgagat gccgacccgc tggtacaacc tcgtgcacga cctgccgacg ccgccgccgc
       61 cttacctgca ccccggcacg ggcctgccgg tcaccccgga cgacctcacg ccgctgttcc
      121 cgatggcact catcgagcag gaggtcacga ccgagcagta tgtcgagatc cccggcgagg
      181 tcctcgacgt ctaccggctc tggcggccgt cccccctcta ccgggcccac cgcctggaga
      241 aggcgctcgg cacgcccgcg cggctctact acaagtacga gggcgtctct cccgctgggt
      301 cgcacaagcc gaatacctcg gtgccgcagg cgttctacaa ctcccaggcc gggatccgca
      361 agctcaccac cgagaccggc gccggccagt ggggcaccgc actcgccttc gcatgcgcgc
      421 agttcgacct ggagtgcgag gtgtggcagg tccgggcctc gtatgactcc aagccctacc
      481 gccgcatgat gattgagacc ttcggcggga ccgtgcaccc gagcccgtcg tcgctcaccg
      541 ccgccggtcg cgcgatcctc gccaaggacc ccggctcccc agggtcgctc ggcatcgcga
      601 tctccgaggc cgtcgaggtc gccgcccaga gccccgacac gaactacgcc ctgggctcgg
      661 tcctgggcca cgtgctgctc caccagacga tcatcggcga ggaggcgctg ctgcagctgg
      721 ccaagatcgg cgagagcgtc gacctcatcg tcggctgcac cggcggcggg tcgaacttcg
      781 gcgggctcac cttcccgttc ctgcgcgaga agctcgccgg gcggatgtca ccggtgatcc
      841 gggccgtcga gcccgcctcg tgcccgtcgc tgacccaggg cacctacgcc tacgacttcg
      901 gcgacacggc cgggatgacg ccgctcatga agatgcacac cctgggtcat gacttcatcc
      961 ccgacccgat ccacgccggc gggctgcgct accacgggat gagcccgctc atctcgcacc
     1021 tcgtggagga gggcttcatc gaggcgatcg ccaagacgca gcaggactgc ttcgccgccg
     1081 gcgtccagtt cgcccggacc gagggcatcc tgccggcgcc cgagcccacc cacgcgctgg
     1141 ccgcggccat cgacgaggcg ctgcggtgca aagagaccgg cgaggagaag gtcatcctca
     1201 ccgccctgtg cggacacggt cacctcgacc tgcccgcgta tgacgcgtac ttgtccggca
     1261 cgctcgtcga ccacacctgg gccgacgcgg agctcaaggc atcggtcgcc gaggcactga
     1321 cccacctgcc cgccataccc ggctgagctc acgccagggc gatgaggtcc aggtagtccg
     1381 ggccccacag gtcctcgacc ccgtcgggca ggagcaggat ccgctcgggc tcgagggcct
     1441 ggaccgcccc ctcgtcgtgg gtcacgagga cgaccgcgcc cttgtaggtc cgcagcgccc
     1501 cgaggatctc ggcccgggac gcggggtcga ggttgttcgt cggctcgtcg agcaggagca
     1561 cgttggcggc cgagaccacc agcgtggcca gcgacagccg ggtcttctcg ccgcccgaga
     1621 gcacgccggc cggcttgtcg acatcgtccc cggtgaacag gaacgacccg agcacgttac
     1681 ggacctcggt ctcgcccagg tcaggcgccg cggtcttcat gttctccagc accgagcggg
     1741 acacatcgag gttctcgtgc tcctgggcgt agtagcccag cttgagcccg tgaccggcct
     1801 cgaccacacc ggtgtcgggc gggtcgatgc cgccgaggat ccgcagcagg gtcgtcttgc
     1861 ccgccccgtt gaggccgagg accacgacgc gcgacccgcg gtcgatggcc aggtcgacgt
     1921 cggtgaacac ctcgagcgac ccgtaggagc gggacagccc cgttgccgtc agcggcgtct
     1981 tgccgcaccg ggccggtgac gggaaacgca gcttggccac ccggtcgtgg acccgctccc
     2041 cctcgatgga cgcgagcagc cgctcggccc ggcgggccat gttctgcgcg gccaccgcct
     2101 tggtcgcctt ggcccgcatc ctgtcggcct gggcgagcag gacggaggcc ttcttctcgg
     2161 cgttgagccg ctcccgccgg cggcgccgct cgtcggtctc gcgcgcgagc aggtacgcct
     2221 tccacccgac gttgtagacg tcgagctcgg cccggttggc gtcgagatgg aacacccgcg
     2281 tcacgacctg gtcgagcagc gcgacgtcgt gggagatgac gaccagacca cccttgtggg
     2341 ccttgagatg gtcccgcagc cagaccaccg agtcggcgtc gaggtggttc gtcggctcgt
     2401 cgagcagcag cgtcgaggca ctggagaaca ggatccggga cagctcgacc cggcgccgct
     2461 gcccaccgga cagggtgccc aggggctggc cgagcacccg ctcgtcgaga cccagcgccg
     2521 aggcgatcgc cgccgcctcg gactcggcgc cataccctcc cgcggcctgg aactcggcat
     2581 cgaggcgcgc gtagcgcgcc atggccttgt cgcgcacggc ctcgtccagc gacgccatcc
     2641 ccttctcggc ctcgcgcagg cggtgcacga tgacgtcgag tccccgggcg gcgagaatcc
     2701 ggtcccgcgc ccgggcgtcg aggtcgccgg tacgcgggtc ctgggcgagg taccccaccg
     2761 tcccggacac gacgaccgac ccggcggcgg gtgggccttc accagcgagc acccgggtca
     2821 gtgtcgtctt gccggctccg ttgcgcccga ccagccccac ccggtcgccg tcgttgatgc
     2881 ggaacgtcgc gccgtcgagg agcagccgcg atcccgcgcg cagctcgacg gcggtggcgg
     2941 cgatcacggc atactcccga cgtggtggcg cgcccccgcg tcccgggggc aaaccatccc
     3001 agtgtgccag gccgcacggc accctcccaa cccgacgccc agccgaggtc gacccgcgac
     3061 tggcgtgcgg cacgaggggc aggccgggag cgttagggtg ggcggcagca gccgcgggcc
     3121 accacgcccc ccgaagtcct ggagcagcac atgagcttca ccgagaacgt ccagctcgac
     3181 acctcacgga tcgagtccgg cgggggtggc cgcgggctcg ccgggatcaa ggtcggcggc
     3241 ggcatcggtg gcgtcatcgt catggtcgtc gtcgtcctct tccaggtgct cggcaacggc
     3301 ggaggtggca gcggcggaac gctccccatc gacgcggcgc agtacgacgc gagccgcgtc
     3361 gcggcaggcg ggcaggtcga cccggccgcc ttctcccagt gcaggaccgg cgcagacgcc
     3421 aaccgcgacg acgtctgccg catcatcgcc acggtcaact cggccgagcg gttctgggcc
     3481 gaggaactgc cccggagagg cctgaggttc cggcccgccc agaccgtcat ctacgacggc
     3541 acgacccagt cggcctgcgg cacggcgtcg aaccaggtcg gcccgttcta ctgccccctc
     3601 gacgagaaga tctacatcga cgcgagcttc ttcgccgagc tcacctcgcg gttcggggcc
     3661 gacgacgggg cgctggccaa ggagtatgtc gtggcccacg agtacggaca ccacatccag
     3721 aacatcctcg acatcctcgg ccgcgcccaa caggacccgc aggggccgga aagcggagcg
     3781 gtgcggatcg agctcatggc cgactgcctc ggcggcatgt gggtcaagaa cgcgacgacg
     3841 acgaccgacg cccagggggt gacgtt
//
LOCUS       DATAIX010000071         1644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_269803, whole genome shotgun sequence.
ACCESSION   DATAIX010000071 DATAIX010000000
VERSION     DATAIX010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269803"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="VHM65_01135"
     CDS             <1..1090
                     /locus_tag="VHM65_01135"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX2704335.1"
                     /translation="VTIKQVELTTIEQAFATGGVIAHPPDRLSGNTVAVVGSGPAGLA
                     VAQQLTRAGHTVAVYERDDAAGGLLRYGIPEFKMEKAVLERRIAQMEAEGTRFRTSVR
                     VGEDVTGKDLRERYDAVVLAIGATVPRDLPVPGRELGGILQAMEYLPPANRVALGRQA
                     EGRVSAKGKDVIVIGGGDTGSDCLGTALRQGARSVTQLEILPRPTEDRPASQPWPTYP
                     MTYRVSSAQEEGGDRVFSVITKELLGDDAGQVRALRLVDVEGSEREIPAQLVLLAMGF
                     LGPQPGGLVEQLGVELDERSNITRDASFMSSEPGVFVAGDAGRGQSLIVWAIAEGRSA
                     AHGVDAWLTGVPSGLPRPINPTDRPLSI"
     gene            1206..1568
                     /locus_tag="VHM65_01140"
     CDS             1206..1568
                     /locus_tag="VHM65_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX2704336.1"
                     /translation="MQKQAIELSRRYAKPVIVATQVLDSMIESSRPTRAEASDCANAV
                     LDGADALMLSGETSTRPVAGRGRPHDGPHHREHRGPRARAHRAARGRPADDRVRALQG
                     RYAGSRHLATHPAGMVER"
     gene            1548..1633
                     /locus_tag="VHM65_01145"
     tRNA            1548..1633
                     /locus_tag="VHM65_01145"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1581..1583,aa:Leu,seq:caa)
BASE COUNT          253 a          585 c          576 g          230 t
ORIGIN      
        1 ggtgacgatc aagcaggtgg agctgacgac gatcgagcag gcgttcgcga ccggcggcgt
       61 catcgcgcat ccgcccgacc gcctctcggg caataccgtc gccgtcgtgg gctccggacc
      121 ggccggcctg gctgtcgccc agcagctgac ccgcgccggt cacaccgtgg ccgtctacga
      181 acgcgacgac gccgcaggcg gcctgctgcg ctacgggatc ccggagttca agatggagaa
      241 ggcggtcctc gagcgacgga tcgcccagat ggaggccgag ggcacgcggt tccgcacgag
      301 cgttcgcgtc ggtgaggacg tcaccggcaa ggacctgcgg gagcggtatg acgcggtcgt
      361 gctcgcgatc ggggccaccg tcccgcggga cctgccggta cccgggcgag agctgggcgg
      421 gatcctgcag gcgatggagt acctccctcc ggcgaaccgg gtggcgctgg gccgccaggc
      481 cgagggccgc gtcagtgcca agggcaagga cgtcatcgtc attggtggcg gtgacaccgg
      541 ctccgactgc ctcggcaccg cgttgcgcca aggcgcccgg tcggtcaccc agctcgagat
      601 tctcccgcga ccgaccgagg accgaccggc gagccagccg tggccgacat accccatgac
      661 ctaccgcgtg agcagcgccc aagaagaggg gggcgaccgg gtcttctccg tgatcaccaa
      721 ggagctcctc ggcgacgacg cggggcaggt ccgggccctg cgcctcgtcg acgtcgaggg
      781 cagcgagcgg gagatccctg cccagctcgt cctgctcgcc atgggcttcc tcgggccgca
      841 gccagggggg ctggtcgagc agctcggcgt cgagctcgac gagcgctcga acatcactcg
      901 ggatgcctcg ttcatgtcgt ccgagccggg cgtgttcgtt gccggtgacg ccgggcgtgg
      961 ccagtcgctc atcgtctggg ccattgccga aggccggtcc gccgcccatg gggtggacgc
     1021 ctggctgacc ggcgtgccct cggggctccc gcgcccgatc aacccgaccg accggccgct
     1081 cagtatctga gaagcgaccg tctgactgtc gccaacgtca cagcgcggct caacctctcg
     1141 cacggcgagt gctccacgca cgagcaggtc ttggcgtcga gctcccgctc gagcaggtcc
     1201 cgctcgtgca gaagcaggcc atcgagctgt cccggcgcta tgccaagccg gtcatcgtcg
     1261 cgacgcaggt gctcgactcg atgatcgaga gctcgcgccc gacccgcgcc gaagcctccg
     1321 actgtgccaa cgccgtcctc gacggcgccg acgcgctcat gctgtcgggg gagacctcca
     1381 ctcggccagt ggccggtcga ggccgtccgc acgatggccc gcatcatcga gaacaccgag
     1441 gaccacgggc tcgagcgcat cgcgcggctc ggggccgtcc cgcagacgat cgagtgcgag
     1501 cactccaggg ccggtatgct ggcagccgcc acttggccac ccaccctgcc gggatggtgg
     1561 aacggtagac acggagcact caaaatgctc tgcccgcaag ggcgtgcggg ttcgagtccc
     1621 gctcccggca ccactacgct tgag
//
LOCUS       DATAIX010000072         1619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_33689, whole genome shotgun sequence.
ACCESSION   DATAIX010000072 DATAIX010000000
VERSION     DATAIX010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33689"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="VHM65_01150"
     CDS             <1..1115
                     /locus_tag="VHM65_01150"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2704337.1"
                     /translation="DSPRRTYDTASAPAPAQSTVPRGVRVTSEWAWRLLVIGAAALAV
                     PYALRGLSELTVPLAIAMLLSALLYPLFLWLRRRLSPGPAAGLSVLALVIVVAGLLTL
                     VGSQFSGGLTDLTSQVSSGMVEVRDWARTTFKISDTQFDAYFDQLREAVTSSGDLKGM
                     ATKTGLTATHAIAGLFIALFATFFFLYEGSRIWAWLMRLVPSGGRTKIDTSGHLAWGQ
                     LTAFVKATIVVAAVDATGIAVGAAILRVPFAGAIFVIVFLGSFVPIVGALVSGAVAIL
                     LALVTHGPVVALIMTAVVIGVQQVEAHVLQPFLLGRAVQVHPLAVILAIAAGVIIAGI
                     VGALIAVPTAAVLNAVGKHLLLDPTEPAPSEAEAVV"
     gene            complement(1112..>1619)
                     /locus_tag="VHM65_01155"
     CDS             complement(1112..>1619)
                     /locus_tag="VHM65_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704338.1"
                     /translation="VSRPPVRAVVGVLLLAALVGLPATVSLAVVGRLTWLGVLLDLVA
                     LLGLSLVLRRAGVRRRAARRSLARRHTGGSSSVLDQVGTTPGERSATGPAAEADGGAA
                     VAGPSVEADPVVADPNAWTPVPVPPPTYMLKAKAVYREPPPAPVPASAPAPILTAVPG
                     AESDIRAV"
BASE COUNT          210 a          571 c          590 g          248 t
ORIGIN      
        1 cggacagccc gcgcaggacg tacgacacgg ccagcgcccc ggcgccagcc cagtccaccg
       61 tgccgagggg cgtgcgtgtc acgagcgagt gggcctggcg gctgctcgtc atcggagctg
      121 ccgcgctggc cgtgccctac gccctgcgcg ggctgtccga gctcaccgtc ccgctcgcca
      181 tcgcgatgct gctcagcgcg ttgctctacc cgctgttcct ctggctgcgc cgacggctca
      241 gcccgggccc ggcggccggc ctgagcgtgc tcgctctggt catcgtcgtt gcggggctgc
      301 tcaccctggt cgggtcgcag ttctccggcg gcctgaccga cctgacgagc caggtgtcca
      361 gcgggatggt cgaggtgcgc gactgggcgc ggacgacgtt caagatcagc gacacccagt
      421 tcgacgccta cttcgaccag ctccgcgagg cggtgacgtc cagcggcgac ctcaagggta
      481 tggcgacgaa gaccggcctc accgcgaccc atgcgatcgc ggggctcttc atcgcgctgt
      541 tcgcgacgtt cttcttcctc tacgaagggt cgcggatctg ggcctggctg atgcggctgg
      601 tgccgtcggg cgggcgcacc aagatcgaca cgtcgggtca cctcgcctgg ggccagctca
      661 cggcgttcgt caaggccacg atcgtggtgg ccgcggttga cgcgacgggg atcgccgtgg
      721 gggcagcgat cctgcgggtg ccgtttgccg gggcgatctt cgtcatcgtc ttcctcggct
      781 ccttcgtgcc gatcgtcggg gccctggtca gtggggccgt cgccatcctg cttgccctgg
      841 tcacccacgg cccggtcgtc gcgctcatca tgacggccgt cgtcatcggc gtgcagcagg
      901 tggaggcgca cgtgctgcag cccttcctgc tcgggcgcgc cgtgcaggtg cacccgctcg
      961 ccgtcatcct cgcgatcgcg gcgggcgtca tcatcgcggg cattgtgggg gccctgatcg
     1021 ccgttcccac cgccgcagtc ctcaacgccg tgggcaagca cctgctgctc gaccccactg
     1081 agccggcccc gagcgaagcc gaggccgtcg tctagaccgc gcggatgtcg gactcggcac
     1141 cgggcaccgc ggtgagaatg ggcgccgggg ccgatgcggg caccggagcg ggagggggtt
     1201 cgcggtagac ggccttggcc ttgagcatgt aggtcggtgg cggcactggg acgggcgtcc
     1261 acgcgttggg gtcagccaca accgggtcgg cctcgactga cgggcctgcg acagcggcgc
     1321 caccgtcggc ctctgctgcg gggccggtcg cgcttcgctc acctggggtt gtgcccacct
     1381 ggtcgaggac ggaggaagac cctcctgtat gacggcgcgc aagactgcgt cgcgccgcac
     1441 gacgacgcac gcccgcacgc cggagcacga ggctcaggcc gaggagtgcg accaggtcga
     1501 gcagcacgcc gagccaggtg agccgcccga cgacagccag cgagaccgtg gccggcaggc
     1561 ccaccagggc tgcgaggagg aggacgccga cgacggctcg caccggcggg cgcgagact
//
LOCUS       DATAIX010000073         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_152872, whole genome shotgun sequence.
ACCESSION   DATAIX010000073 DATAIX010000000
VERSION     DATAIX010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152872"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..523)
                     /locus_tag="VHM65_01160"
     CDS             complement(8..523)
                     /locus_tag="VHM65_01160"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEX2704339.1"
                     /translation="MRRLLVSSLTVAALAAGYATLDAYDRAPGILTLAPVPAQTQSPA
                     ASPSTTPPGMTLPHLPAATTVLPQASQTAPMPTPAGLAAAVKGPLADPSLGQKSVLVR
                     DAATKRLLLSTDPSRARTPASTAKLLSAAAVLDTLGPTTTLTTRVLGPTTEGRIWLVA
                     GGDTLLSPGQG"
     gene            627..1232
                     /locus_tag="VHM65_01165"
     CDS             627..1232
                     /locus_tag="VHM65_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX2704340.1"
                     /translation="MEFDVTIEIPKGHRNKYEVDHETGRIRLDRLLFTSMSYPADYGY
                     IEDTLGDDGDPLDALVLLDEPTFPGCIVRARPIGMFHMRDEAGGDDKIMCLPARDPRR
                     AHVTDLDHLNKFDVLEIQHFFETYKDLEPGKSVEGAHWAGRESAEKCITDALERASSI
                     GMTTARWRLPMGDAGHAGADPGSAAAETLDEDGDDGEDGDQ"
     gene            1435..1638
                     /locus_tag="VHM65_01170"
     CDS             1435..1638
                     /locus_tag="VHM65_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704341.1"
                     /translation="MAVWRVPGLAAAWPAVAIGAATVSAVLLVLFWNWHLVFGLVIDA
                     ALVVLAIVQPRWLDQVMTRGVAA"
     gene            complement(1954..>2405)
                     /locus_tag="VHM65_01175"
     CDS             complement(1954..>2405)
                     /locus_tag="VHM65_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704342.1"
                     /translation="VASEIIALAMGSSPQSLHGGLVLLSDSYAAADTDTQRAALVSAA
                     EALIAATNAVSWAGILTAAAILILSLLMRRSPFGMTMAVIGAVTGTLGIASEALRPMI
                     GVGYLLYGILLPTWFALVGWRLLHLGSRPSGAGAVAASTEPRAGKLT"
BASE COUNT          370 a          774 c          904 g          357 t
ORIGIN      
        1 accctgctca gccctggcca gggctgagca gggtgtcgcc gccggccacg agccagatcc
       61 ggccctcggt ggtgggtccg aggactcggg tggtcagcgt cgtggtgggc ccgagggtgt
      121 cgaggacggc cgcagcggag aggagcttgg ccgtcgaggc gggagtgcgc gcccggctcg
      181 gatcggtcga cagcagaagc cgtttcgtcg ccgcgtcgcg cacgagcacc gacttctggc
      241 cgagggatgg gtccgcgagg ggtcccttca ccgcggccgc gagaccggcc ggggtcggca
      301 tgggagccgt ctggctggcc tggggcagga cggtcgtcgc ggccggaagg tgcggcagcg
      361 tcataccggg gggtgtcgtt gacggtgacg ctgccgggga ctgggtctgg gcgggaacgg
      421 gcgcgagggt gaggatgccg ggcgcccggt cgtaggcgtc cagggtggcg tacccggcgg
      481 cgagggcggc caccgtcagt gacgacacga gcaggcggcg cacggcgtgc atcctccctc
      541 gggacaaacc agccaggagc acccacagta ggacagactg gtccctgcaa ccgaccacag
      601 cgacgacacg gggcaggaga cgcacagtgg agttcgacgt cacgatcgag atccccaagg
      661 ggcaccgcaa caagtacgag gtcgaccacg agaccgggcg gatccggctc gaccggctgc
      721 tcttcacctc gatgagctat ccggccgact acgggtacat cgaggacacc ctcggtgacg
      781 acggtgaccc gctcgacgcg ctcgtgctcc tcgacgagcc gaccttcccc ggatgcatcg
      841 tgcgggcgcg gccgatcggc atgttccaca tgcgcgacga agcaggcggc gacgacaaga
      901 tcatgtgcct gccggcgcgc gacccgcggc gggcccacgt gacggacctg gaccacctca
      961 acaagttcga cgtgctcgag atccagcact tcttcgagac ctacaaggac ctcgagccgg
     1021 gcaagtcggt cgaaggggcg cactgggccg gtcgtgagtc cgccgagaag tgcatcaccg
     1081 acgccctcga gcgggcctcg tcgatcggga tgacgacggc ccggtggcgc ctccccatgg
     1141 gcgacgcggg gcacgctggc gctgacccag ggtccgcggc agcggagact ctcgacgagg
     1201 acggcgacga cggggaggac ggggaccagt agccgtgtag gttctcatcg cacctgtcat
     1261 ctccgatggg gtcgttgtgc tccgcagcgg gcatgttcct cacggtgaca tcgcgcgcct
     1321 tctcatcgag ttggaggcag ccgacgattg ttgcggcgcg cgaaggtcca tggcacccgg
     1381 gcagacaggg tgcagcaggg ttcgcccccg gattctgcct tcgcgttgct ggccttggcg
     1441 gtctggaggg tgccgggcct ggccgccgcc tggccggccg tcgcgatcgg tgcggcgacg
     1501 gtctcggcgg tcctcctcgt gctgttctgg aactggcacc tcgtcttcgg gctcgtcatc
     1561 gacgctgcgc tggtcgtgct cgcgatcgtc cagccgcggt ggcttgacca ggtcatgacc
     1621 cggggtgtgg cggcttgacc acgatgacgc gtcgctccgg acctgctcca ctggggtccc
     1681 cggcaccgct gtgctatcgc ggcgatgcgc gacgtgacgc gtcgcggtgg ctcgttcgac
     1741 gagatcgagc accggcccgg ctcgagcgag cgctgaccgc ccaaggcatc ccgaacgacg
     1801 tgacgacata cgagggagct gggcactcct tcctcaacga ccacccaccc agcgagacgc
     1861 ctcgatgcag gccgcggtcg gtgccgagca ccggtgggtg tagtgctgtg ggcccagatc
     1921 gatgtcgaca gcggtggagt cggcgccgcg cggtcaggtt agcttgcccg cgcgtggttc
     1981 ggtgctcgcg gcaacggcgc ctgctcctga gggtcgactg ccgaggtgaa ggagcctcca
     2041 tccgaccagc gcgaaccagg tgggcaggag tattccgtag aggagatagc cgacgccgat
     2101 catcgggcgg agcgcctcgc tcgcgatgcc gagtgtgccg gtgacggcac cgatgaccgc
     2161 catggtcatg ccgaagggac tcctgcgcat gagcagggac aggatgagga ttgcggctgc
     2221 ggtgaggatg ccggcccagg acaccgcgtt cgttgcggcg atgagggctt ccgccgcgct
     2281 gacgagggcg gcccgctggg tgtcggtgtc ggccgccgcg taggagtcgc tcaggaggac
     2341 gagcccgccg tggagggact gagggctgct gcccatggcc agcgcgatga tctccgaggc
     2401 aacac
//
LOCUS       DATAIX010000074         4495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_180598, whole genome shotgun sequence.
ACCESSION   DATAIX010000074 DATAIX010000000
VERSION     DATAIX010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180598"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2035)
                     /locus_tag="VHM65_01180"
     CDS             complement(<1..2035)
                     /locus_tag="VHM65_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX2704343.1"
                     /translation="MSQITRLAILERGEPAVRVLAAVGDENRSSEGPPITTVLAHTEP
                     RPRPWFAREADEVLVLRAGDAGSSLGHDQAASPAYGAADVVASLTAAQIDAVWVGFVP
                     CHDQGELVAACEAAGITVVGPDSATVRRLADPAQLAALVSEAGMALAPDGLDLAPLRG
                     LEVDIIADSSGTVWALDLRAVSLVRGAELVIAETPAPEVPAVTAAAIQAAARALAAGA
                     GYRGAGVVRFVLHPSSLQFWCTGVDTLARPEHALIEETTGASYLGLRLRLALGGRLDP
                     EPPTVDGHAVAARLLARDPDRAFASSGGSIELLTLPAGTGVRVDASIREGDIVVADVD
                     PLIATVTAWGHDRDEALARLRRALERTAAVLEIGATNRSGLLALLSSHEVAAGRVTLG
                     WYDDALASGALRPAADPVAVLAAAVEAYEADLAMVRASFFASAERGRPEHPEAVGSNM
                     SLSYRGAEYRLRVDRTAPGSYRIHGGVSVDVSVDRLGEFERRISCGGRRHAIVAVEQG
                     ADFRIEVDGVAHTVTRGDGVVVRTGWPALVSQVLVEPGQNLTAGQPVAVLESMKMVST
                     VTAPFDGMVTSVAVIANAQVERGAPLLRIRATTAHHLAAAEGADGTGGAPAVPVDFAP
                     FAVTDDSPSGKGTSWVFQRLVDYLLGYDLDPARLRALVAEHKLLSTSLAPDDPA"
     gene            complement(2140..>4495)
                     /locus_tag="VHM65_01185"
     CDS             complement(2140..>4495)
                     /locus_tag="VHM65_01185"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2704344.1"
                     /translation="TALTVEPGIATEDLAARLDWWLGEGPIAGVYWLSALDNEGSIDA
                     LDLAGWTEALRRRVKTLYATMRRMWDTGPFLVSATRLGGYHGYDAGGATCPLGGAVTG
                     FTKSYKKERPEALVKAVDLPVSRRTATLADLLVEETLRDPGCVEIGRVDDQRWGVAFA
                     EVPFPALGADGSPDGEGGMTLTKDTVALVTGAAGSIVSAITADLAKASGGTFHLLDLT
                     PVPDPADPDLLAFRTDKAGLKATIAARLKEGGKRATPVAIDKELARLERLGAALTAIQ
                     SVEAAGGTAHYYSVDLTNGEAVTAVMEQVRERSGRIDILLHAAGLEISRNLPEKEPRE
                     YDLVFDVKANGWFNVWRAAATMEVGATVAFSSVAGRFGNNGQTDYSAANDLLCKIASA
                     TRRTRPQTRALALDWTAWGGIGMATRGSIPKIMEMAGVQMLPPEAGVAWIRRELASSS
                     YSGEVIVAGVLGAMATEYYPGGGADPAALIGDQAHGPMVGEVSSSVYSGLVVRTTLDP
                     KAQPFLDNHRIDGTAVLPGVMGMESFAEAAALLAPAGYRVGSVEDVGFAAPVKFYRDA
                     PRTLTVSAVASPDPAGTGDLLVHCRLTGERTLPGSDVPATTVHFTGTVRLTQAEVTAE
                     TRRVSTTPADTALSSDQVYSFYFHGPAYQVVDAAWRTGDRSVARLRDPLPDNHTPSEW
                     PLAIAPRLAELCFQTAGLWEAGRDERLALPMRVDRLVVLRDPATVKGSLFGVARQSGP
                     STFDCAVIDSMGKVFMRMDGYHSIAFPSVIPDEVAGALRSTFAD"
BASE COUNT          623 a         1593 c         1683 g          596 t
ORIGIN      
        1 gtgctgggtc gtcgggggcg agcgaggtgg agaggagctt gtgctcagcc acgagcgccc
       61 gcaggcgggc cgggtcgagg tcgtagccca gcagatagtc gaccagccgc tggaagaccc
      121 acgacgtgcc cttgccggac ggcgagtcgt cggtcacggc gaagggggcg aagtccaccg
      181 gcaccgcggg cgctccgcca gtgccgtccg ccccctcggc ggcagccagg tggtgggccg
      241 ttgtcgcgcg gatccgcagc agtggcgcgc cccgctcgac ctgggcgttg gcgatgacgg
      301 cgaccgaggt caccatgccg tcgaacgggg ccgtcacggt ggagaccatc ttcatcgact
      361 cgagcacggc gaccggctgc ccggcggtga ggttctggcc tggttcgacg aggacctggg
      421 acaccagggc cggccacccg gtccgcacga cgacaccgtc gccgcgggtc acggtgtgag
      481 cgactccgtc gacctcgatg cggaagtcgg caccctgctc gacggcgacg atggcatggc
      541 gccgcccgcc gcaggagatc cgccgctcga actcgccgag ccggtcgacg ctgacgtcga
      601 cgctgacacc gccatggatc cggtaggacc ctggggcggt gcggtcgacc cgcagccggt
      661 actcggcgcc gcggtaggac agggacatgt tcgaccccac ggcctcgggg tgctcggggc
      721 gaccccgctc ggcgctggca aagaacgatg cgcggaccat agccaggtcg gcctcgtagg
      781 cctcgacggc ggccgccagc acggccaccg ggtccgccgc aggccgcagc gcacccgagg
      841 cgagggcgtc gtcgtaccag ccgagagtga cccggccggc ggccacctcg tggctggaca
      901 gcagggccag cagcccggag cggttggtcg ctccgatctc caggaccgcg gccgtgcgct
      961 ccagcgcccg gcgcagccgg gccagggcct cgtcgcggtc gtgtccccag gccgtcacgg
     1021 tcgcgatgag cgggtcgacg tcggcgacca cgatgtcacc ctcgcggatg ctcgcgtcga
     1081 ccctgacccc cgtgccggcc ggaagggtca gcagctcgat cgagccaccg gaggatgcga
     1141 aggcccggtc tgggtcgcgg gcgaggagcc gcgcggccac cgcgtggccg tcgacggtcg
     1201 gcggctccgg gtcgaggcgc ccgccgaggg ccaggcgcag ccgcagcccg aggtagctcg
     1261 ccccggtcgt ctcctcgatg agagcgtgct ccgggcgcgc gagggtgtcg acaccggtgc
     1321 accagaactg cagcgacgag gggtgaagga cgaaccggac caccccggcc ccgcggtagc
     1381 cggcccccgc cgcgagcgcc cgggccgcgg cctggatcgc cgcagccgtc acggccggaa
     1441 cctcaggcgc cggggtctcg gcgatgacga gctcggcgcc gcggacgagg ctcaccgcgc
     1501 gcaggtcgag cgcccagacc gtgcccgacg agtccgcgat gatgtcgacc tcgaggcccc
     1561 gcagcggcgc gaggtcgagg ccgtccggcg ctagcgccat accggcctcg gacacgagcg
     1621 cggcgagctg agccgggtcg gcgaggcgcc gcaccgttgc actgtccggg cccacgacgg
     1681 tgatcccggc agcttcgcag gccgcgacga gttcgccctg gtcgtgacat ggcacgaacc
     1741 ccacccacac ggcgtcgatc tgggcagccg tgagcgacgc gacgacgtcg gccgcgccgt
     1801 aggccgggct cgcagcctgg tcatggccaa gggatgagcc cgcgtcgccc gcgcggagca
     1861 cgagcacctc gtcggcctcc cgggcgaacc acggccgggg gcgaggttcg gtgtgcgcga
     1921 gaaccgtcgt gatggggggg ccttcactgg accggttctc gtcgccgacc gccgcgagga
     1981 ctcggacggc gggctcgccc cgctcgagga tggcgagacg ggtgatctgg ctcatggggc
     2041 tctccgatgg gtggttgttg gctccgggtc agggtggacg tgatgcccga ggatcggtcg
     2101 tcgatcggtc gggcaccacg tccgcggggc aggtggagat cagtcggcga aggtactccg
     2161 gagtgctccg gcaacctcgt ccgggatgac cgacgggaag gcgatcgagt ggtagccgtc
     2221 catccgcatg aacaccttgc ccatggagtc gatgaccgca cagtcgaagg tgctcggtcc
     2281 gctctggcgc gcgacgccga acagggagcc cttgacggtc gccgggtcac gcaggaccac
     2341 cagccgatcg acccgcatcg gcagggcaag gcgctcgtca cgcccggcct cccacagacc
     2401 cgccgtctgg aagcacagct cggccaggcg cggggcgatg gcgagcggcc attccgacgg
     2461 ggtgtggttg tccggcagcg ggtcgcgcaa ccgcgcgacc gaacggtctc cggttcgcca
     2521 cgcggcgtcg acgacctggt aggccggtcc gtggaagtag aacgagtaga cctggtcgct
     2581 gctcagcgcc gtgtcggccg gtgtggtcga gaccctgcgg gtctcggccg tcacctcggc
     2641 ctgggtcagc cgcacggtcc ccgtgaagtg cacggtcgtc gcaggcacgt cgctgcccgg
     2701 cagcgtgcgc tcgccggtga gccggcagtg cacgagcagg tcgccggtgc cggccgggtc
     2761 gggcgaggcc acggccgaga ccgtcagcgt ccgcggcgcg tcccggtaga acttgaccgg
     2821 agccgcgaag cccacgtcct cgaccgagcc cacccggtag ccggccggag cgagcagcgc
     2881 ggccgcctcg gcgaaggact ccatccccat gaccccgggc aggacggctg tcccgtcgat
     2941 gcggtggttg tcgaggaagg gctgggcctt ggggtccagc gtcgtgcgca cgacgagacc
     3001 gctgtagacg ctcgagctga cctcgccgac catcgggccg tgcgcctggt ctccgatgag
     3061 cgccgccggg tctgccccgc cgccggggta gtactcggtc gccatcgcgc cgaggactcc
     3121 cgcgacgatc acttcgccgc tgtatgacga cgacgccagc tcccggcgga tccaggccac
     3181 acccgcctcc ggcgggagca tctggacgcc ggccatctcc atgatcttgg ggatggaccc
     3241 gcgggtcgcc atgccgatgc cgccccatgc cgtccagtcc agcgcgagcg cgcgggtctg
     3301 cgggcgggta cgccgggtgg ccgacgcgat cttgcagagc aggtcgttgg cggccgagta
     3361 gtcggtctgg ccgttgttgc cgaaccgtcc ggcgacggac gagaaggcca ccgtggcccc
     3421 gacctccatc gtcgcggcgg cgcgccatac gttgaaccag ccgttggcct tgacgtcgaa
     3481 gacgaggtcg tactcgcgcg gctccttctc cggcaggtta cggctgatct ccaggcccgc
     3541 agcatgcagc aggatgtcga tgcgcccgct gcgctcgcgg acctgctcca tgaccgctgt
     3601 cacagcctcg ccgttggtga ggtcgaccga gtagtagtgc gcggtcccgc ccgcggcctc
     3661 gacggactgg atcgccgtca atgccgcacc gagccgctcg agccgggcca gctccttgtc
     3721 gatggcgacc ggggtggcac gcttgccgcc ctccttgagc cgggcggcga tggtggcctt
     3781 gaggccggcc ttgtcggtcc ggaaggcgag caggtcgggg tcggccgggt caggcaccgg
     3841 ggtgaggtcg agcaggtgga aggtcccgcc gctcgccttg gccagatcgg cggtgatcgc
     3901 cgagacgatg ctgcccgcgg ccccggtgac cagggccacc gtgtccttgg tcagcgtcat
     3961 accgccctca ccgtcggggc tcccgtcggc gccgagcgcc gggaagggca cctcggcgaa
     4021 cgcgacgccc cagcgctggt cgtcgacccg gccgatctcg acgcagccgg ggtcgcgcag
     4081 cgtctcctcg acgagcaggt cggcgagcgt tgcggtccgg cggctcacgg gaaggtcgac
     4141 cgccttgacg agggcctccg gacgctcctt cttgtaggac ttcgtgaagc cggtgaccgc
     4201 gcccccgagc gggcaggtcg ccccgccggc gtcgtagccg tggtagccac cgagccgggt
     4261 cgccgacacg aggaaggggc ccgtgtccca catccggcgc atcgtcgcgt agagcgtctt
     4321 gacccgtcgg cgcagggcct cggtccagcc ggccaggtcg agcgcgtcga tcgagccctc
     4381 gttgtcgagc gcggagagcc agtagacccc tgcgatcggg ccctcgccga gccaccagtc
     4441 gaggcgggcg gcgaggtcct ccgtggcgat gcccggctcg acggtcagcg cggtg
//
LOCUS       DATAIX010000075         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_120933, whole genome shotgun sequence.
ACCESSION   DATAIX010000075 DATAIX010000000
VERSION     DATAIX010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120933"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VHM65_01190"
     CDS             complement(<1..501)
                     /locus_tag="VHM65_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX2704345.1"
                     /translation="MVVISTRKWLVQASVSSSDLASLKKGLQAEVTPSGSRTKVFGTV
                     SSVGVIASSSSGVATFPVTIAVTGSPTGLYVGGTAGVAIIVKALTDVLTVPTSAIHTE
                     NGLTVVHQVKNGATVTTPVTLGLVSGTTTQVTKGLSLGDEVVVSTSSFGAGSGSTGTR
                     TRGTGTF"
     gene            complement(559..1074)
                     /locus_tag="VHM65_01195"
     CDS             complement(559..1074)
                     /locus_tag="VHM65_01195"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX2704346.1"
                     /translation="MAVAAALVVVAVGAATAWAVTRPKGDATSAATTVSVTATTLRQT
                     VTSSGTFEPASQADLSFGASGTVTAVKVGVGDSVAAGQVLAIIDSTDLANAVELAQAN
                     LDAASAQLSQSRSSGASSTQIAAAQAQVAAARAKVTSATASLKAATLTAPMAGIVATI
                     SISTGSQVGSS"
BASE COUNT          230 a          616 c          579 g          247 t
ORIGIN      
        1 gaaggtgccc gtcccccgcg tccgggtgcc ggtcgagccg gacccggcgc cgaacgagct
       61 cgtcgacacg acgacctcgt cccccagcga caggcccttg gtgacctgcg tcgtcgtgcc
      121 gctgaccagc ccgagcgtga ccggtgtggt gaccgtcgcg ccgttcttga cctgatgcac
      181 gaccgtcagg ccgttctccg tatggatggc cgaggtcggc acggtcagga catcggtgag
      241 cgccttgacg atgatggcga cgccggccgt cccgccgaca tacaaaccgg tgggcgagcc
      301 ggtcaccgcg atggtgaccg ggaaggtggc gacgccgctg cttgacgagg cgatgacacc
      361 gaccgagctg accgtcccga acaccttggt gcgcgacccg ctgggggtga cctcggcctg
      421 gaggcctttc ttgagcgagg cgaggtccga gctgctgacc gatgcctgga cgagccactt
      481 cctcgtcgag atgacgacga tctgggccga cgagctcgtc gaggccgccg agctcccggc
      541 accgccgaca gcggcgcctc atgatgagcc gacctgggac ccggtcgaga tactgatcgt
      601 tgccacgatg ccggccatcg gcgcggtgag cgtcgccgcc ttgaggctgg ccgtggcgct
      661 ggtgaccttg gcccgggccg ccgccacctg cgcctgggcc gccgcgatct gggtcgatga
      721 cgcgccgctg gagcggctct ggctcagctg ggccgatgct gcgtcgaggt tggcctgggc
      781 cagctcgacc gcgttggcca ggtcggtcga gtcgatgatg gccaggacct gcccggcggc
      841 caccgagtca ccgacgccga ccttgaccgc ggtgaccgtg ccactggccc cgaaggacag
      901 gtccgcctgc gacgccggtt cgaaggtgcc gctcgacgtc acggtctggc gcagtgtcgt
      961 cgcggtgacg ctcaccgtgg tcgcagccga ggtggcgtcg cccttggggc gggtcaccgc
     1021 ccaggcagtc gccgcgccga cggccacgac aacgagtgcc gcggcgacgg ccacccgacg
     1081 tcgcctcgat ggctcgcgtc ctgtcctggc catcgatcca cacccgctcc cgcccggtcg
     1141 ctggacccga gctcatcgac tgagcacctc gcgggcccct gcacccgatc tcagcacgct
     1201 gccggggcct cgcccaggct aggagggccc gcccgccaat tgggtatcac cagcctgtga
     1261 ggaggctgtg aaccgcacca ggaaccgcga ggccgctcgg cgcgtggggg ataccgctcg
     1321 gcgtgttcgt ggggtccgac accgacgcgg ccctccgccc ggctacgggc ctcttggcac
     1381 ccttcgcgat cacgatgtac taccggcgct gggccgaccg tgagttcgac caggccgcgg
     1441 cggatctcgc cacccacatg gagcggggga ccgccccggg gccgcccagc gagcgcgctc
     1501 gccaccatcg ctcccggggc gacccaggtc ggcaacacgt cccttcaaca tcggtatctt
     1561 cgcgttcttc gtcctggtgc ccatcgtcat ccgggtctcc cgccagaacg cgaccgccgg
     1621 cgggccacta cgcgcgtggg cgcgccttca ccgggccccc agaacggcat cg
//
LOCUS       DATAIX010000076         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_93385, whole genome shotgun sequence.
ACCESSION   DATAIX010000076 DATAIX010000000
VERSION     DATAIX010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93385"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VHM65_01200"
     CDS             <1..274
                     /locus_tag="VHM65_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX2704347.1"
                     /translation="ELRLLADERSVADLGAPVLVVSQFTLYGDVRQGRRPSWTAAAPA
                     PVAEPVIDALVTALRDRGIEVATGRFGAMMALTSTNEGPFTVLVDT"
     gene            289..615
                     /locus_tag="VHM65_01205"
     CDS             289..615
                     /locus_tag="VHM65_01205"
                     /inference="COORDINATES: protein motif:HMM:NF022184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2516 family protein"
                     /protein_id="HEX2704348.1"
                     /translation="MPVIWDLQNLLLTLLGLAAFGAELFALIDAVRHQDNAYVAAGKR
                     TRTFWLVVLAVAVVVGFVTMGNILASFIGVIAFVAAAVYLADVRPALRQVTGGGGRNQ
                     GPYGPW"
     gene            710..1126
                     /locus_tag="VHM65_01210"
     CDS             710..1126
                     /locus_tag="VHM65_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HEX2704349.1"
                     /translation="MTRISPRYEPGEYDGGDRDPRADIFRAERPWGHFQQFVSNETVT
                     VKIISVEPGHRLSLQRHGHRGEMWQVLDEPMDIEVDERRWVAQRGATVWVPHGAMHRI
                     GNSGSRTGRILEVAFGYFDEGDIERVEDDYQRHDDA"
     gene            complement(1123..>1542)
                     /locus_tag="VHM65_01215"
     CDS             complement(1123..>1542)
                     /locus_tag="VHM65_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704350.1"
                     /translation="DVWRQVQDRLAPGGLFVDGTSDEAGRLATWVGMDVSGPQTLTLS
                     MRLGGLTRPSTIAERLPKTLIHRNVPGEPVHAVLTALDQAWARAAPHASYGARQRFLV
                     TVRALAEQGWDVRDGPARWRLGELTVGWATVAPRSSV"
BASE COUNT          222 a          552 c          544 g          224 t
ORIGIN      
        1 cgagctgcgc cttctcgccg acgagcgcag cgtcgccgac ctcggggcgc ccgtgctcgt
       61 cgtcagccag ttcaccctgt acggcgacgt ccgtcagggc cggcggccgt cgtggaccgc
      121 ggcggcgccc gccccggtgg ctgagccggt catcgacgcg ctcgtcacgg cgctgcgcga
      181 tcgggggatc gaggtggcaa ccggccggtt cggggcgatg atggcgctca cgtcgaccaa
      241 cgaaggcccc ttcaccgtgc tggtggacac ctgacctact ctggtgtcat gcccgtcatc
      301 tgggacctcc agaacctcct gctcaccctg ctcggcctgg ccgcgttcgg ggccgagctg
      361 ttcgcgctca tcgacgcagt tcgccaccag gacaacgcgt atgtcgcagc gggcaagagg
      421 actcggacgt tctggctcgt agtgctcgcc gtcgcggtcg ttgtggggtt cgtcacgatg
      481 ggcaacatcc tcgcctcctt catcggcgtc atcgccttcg tcgccgccgc ggtctacctc
      541 gccgacgtcc gcccggccct gcgccaggtc accggcgggg gcggtcgcaa ccagggtccc
      601 tacggcccct ggtagcgacg cgcagccctc accgagacgc cgccgtcgca tcgagtaggg
      661 cagactgggt gagccggcct cccgctggcg ctccgccgaa gaaggcttca tgacgcgcat
      721 cagtccccgc tacgagccgg gcgagtatga cggcggtgac cgggacccgc gggcggacat
      781 cttccgggcc gagcgcccgt gggggcactt ccagcagttc gtgtcgaacg agaccgtgac
      841 cgtcaagatc atcagcgtcg aacccggtca tcggctctcc ctgcaacgac acgggcaccg
      901 cggtgagatg tggcaggtcc tcgacgagcc catggacatc gaggtggacg agcggcgctg
      961 ggtggcccag cgcggggcaa cggtctgggt gccgcacggg gccatgcacc ggatcggcaa
     1021 cagcggctcg cgcaccggcc gcatcctcga ggtggccttc ggctacttcg acgagggcga
     1081 catcgaacgc gtcgaggacg actaccagcg acacgacgac gcctagacgc tcgacctcgg
     1141 tgccacggtg gcccagccca cggtcagctc cccgagccgc cagcgagccg ggccgtcacg
     1201 cacgtcccag ccctgctcgg ccagtgcgcg cacggtcacg aggaaccgct gacgggcccc
     1261 gtatgacgcg tgcggggcgg cccgcgccca ggcctggtca agggcggtga gcacggcatg
     1321 gaccggctcg ccggggacgt tgcgatggat cagggtcttg ggcagacgtt cggcgatggt
     1381 cgacggccgg gtgaggccgc ccaggcgcat cgacagggtg agggtctggg ggccgctgac
     1441 gtccatgcca acccaggtcg ccaggcgccc ggcctcgtcg ctcgtgccgt cgacgaacag
     1501 cccgccggga gcgagccggt cctggacctg gcgccatacg tc
//
LOCUS       DATAIX010000077         2427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_240119, whole genome shotgun sequence.
ACCESSION   DATAIX010000077 DATAIX010000000
VERSION     DATAIX010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240119"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1005)
                     /locus_tag="VHM65_01220"
     CDS             complement(10..1005)
                     /locus_tag="VHM65_01220"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX2704351.1"
                     /translation="MHASVIGAGSWGTALGAVLAGKGYDVAIWDVDPVPLESIGARHE
                     NSRYLPGIPLPPSLVPVTDLPQALHETGLVVLAVPSHAMRSVATLVRDDLPTSVPICS
                     VTKGIEVDSLMTMSEVLEDVLPLGIHPFLTYLSGPSFAVEVARGMGTAVTVAAHWNRV
                     ARMVQEAFHTPYFRPYTSPDVVGVEIGGCTKNVVAIATGLADGMGFGANARAALITRG
                     LAEITRLGVSRGANPQTLSGLAGIGDLLLTCSSSKSRNYRVGYGLGQGKSLAQIQAEL
                     GQVAEGVLNAKSVHRLAELMRVEMPISEAVYRLIYEDLSPRGVLASLTERDLKGE"
     gene            1126..2346
                     /locus_tag="VHM65_01225"
     CDS             1126..2346
                     /locus_tag="VHM65_01225"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006090.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX2704352.1"
                     /translation="MAGIPDAFVYDAIRTPRGKGKKGGSLYEVKPVDLVVGLLRELGA
                     RHTGFDPARVEDVVLGCVTPIGDQGADIAKTAVIAAGYPDTVGGVQLNRFCASGLDAV
                     NQAAQRVRSGFEDLIIAGGVESMSRVPMASDGGAWALDPATSMATDFVPQGVSADLIA
                     TIEGLSRYDVDAYAAESQRRAAAAWDAGYFSGSVVPVRDRNGLTILDHDELIRPGTTV
                     ESLGALAPSFAAMGQRAGFDAVALEKYHWVDAIDHVHHAGNSSGLVDGAALVLVGTEQ
                     VGAELGLTPRARVVATAVSGSDPTIMLTGPAPAARKALARAGLAPGDIDLYEMNEAFA
                     AVVLRFIRDLGLTPERVNVNGGAIAMGHPLGATGAMLLGTLVDELERRDQRYGLVTLC
                     VGGGMGIATVVERL"
BASE COUNT          357 a          850 c          873 g          347 t
ORIGIN      
        1 gggcttgggc tactcgccct tgaggtcgcg ctcggtcagg gatgccagca cgccacgcgg
       61 gctcaggtcc tcgtagatga gccggtagac ggcctcgctg atcggcatct cgaccctcat
      121 cagctcggcg agacggtgga cgctcttggc gttgaggaca ccctcggcga cctggccgag
      181 ctcggcctgg atctgcgcca gggacttgcc ctggccgaga ccgtagccga cccggtagtt
      241 gcgcgacttc gacgacgagc aggtgaggag caggtcaccg atcccggcca gacccgagag
      301 ggtctgcggg ttggcgcccc ggctcacgcc gaggcgggtg atctcggcga ggccccgggt
      361 gatgagcgcc gcccgggcgt tggcgccgaa gcccatcccg tcggcgaggc cggtggcgat
      421 ggcgacgaca ttcttggtgc agccgccgat ctcgacaccg accacgtccg gggacgtgta
      481 gggacggaag tagggcgtgt ggaaggcctc ctggaccatc cgggcgacgc ggttccagtg
      541 ggccgccacg gtgacggcgg tccccatccc gcgggccacc tcgaccgcga acgacggccc
      601 ggacaggtag gtcaggaacg ggtggatccc cagcggcagc acgtcctcga ggacctcgct
      661 catcgtcatg agcgagtcga cctcgatgcc cttggtcacc gagcagatcg gcacgctcgt
      721 cggcaggtcg tcacggacga gggtcgccac cgaacgcatc gcgtgggagg gcaccgcgag
      781 cacgacgagg ccggtctcgt gcagtgcctg cggcaggtcg gtcactggga ccagcgacgg
      841 tgggaggggg atcccgggca ggtagcggga gttctcgtgg cgggccccga tcgactccaa
      901 cggcactggg tcgacgtccc agatcgcgac gtcatacccc ttgccggcca gcaccgcgcc
      961 aagtgcggtg ccccaggacc ccgctccgat cacgctcgcg tgcatgacca ctccctccac
     1021 cagaacagcc gtcgtgcctg cctccgtcgt gcccgagggt agcgtgctgg acctcacgcc
     1081 gcagcgccat cgagccttcg tcgtggcagg tggcacgatg tgcagatggc tggcatcccc
     1141 gacgcattcg tgtatgacgc gatccgcacc ccgcgcggga agggcaagaa gggcggctcg
     1201 ctctacgagg tcaagcccgt cgacctcgtc gtggggctgc tgcgcgagct cggtgcgcgt
     1261 cataccggct tcgaccccgc cagggtcgag gacgtcgtcc tcggctgcgt gaccccgatc
     1321 ggtgaccagg gtgccgacat cgccaagacc gcggtgatcg cggcgggcta cccggatacg
     1381 gtcggcgggg tgcagctcaa ccggttctgc gcctcggggc tcgatgcggt caaccaggcg
     1441 gcccagcgag tgcggtccgg cttcgaggac ctcatcatcg ccgggggagt cgagtcgatg
     1501 tcgcgcgtgc cgatggcctc ggacggcggc gcctgggcac tggacccggc gacctcgatg
     1561 gcgacggact tcgtgccgca gggggtcagc gccgacctca tcgccacgat cgaaggcttg
     1621 agccggtatg acgtcgatgc gtatgccgcc gagtcgcagc gacgcgcggc ggccgcgtgg
     1681 gacgcggggt acttcagcgg ctcggtggtc ccggtccgcg accgcaacgg gctgacgatc
     1741 ctcgaccacg acgagctcat ccgcccgggc acgacggtcg agtcgctcgg ggcgctggcg
     1801 ccctcgttcg cggcgatggg ccagcgggcc gggttcgacg cggtggcgct ggagaagtac
     1861 cactgggtcg acgcgatcga ccatgtccac cacgccggca actcctcggg cctcgtcgac
     1921 ggggccgccc tggtcctcgt cgggaccgag caggtcggcg ccgagctcgg gctcacgcct
     1981 cgcgcccgcg tggtggccac cgccgtgagc gggtcggacc ccacgatcat gctcaccggc
     2041 ccggcgcctg cggcccgcaa ggcgctggcc agggccgggc tggcccccgg cgacatcgac
     2101 ctctacgaga tgaacgaggc cttcgccgcg gtcgtcctgc gcttcatccg cgacctcggg
     2161 ctgacgccgg agcgggtcaa cgtcaacggc ggggcgatcg cgatggggca ccccctcgga
     2221 gcgaccggcg cgatgctcct cggcaccctc gtcgacgagc tcgaacgtcg tgaccagcgg
     2281 tacggcctgg tcacgctctg cgtcggcggc gggatgggga tcgccaccgt cgtcgagcga
     2341 ctctgaccgc actcccacgg cactttcgag cccgcttcgc cgatcgcaag ggacaccacg
     2401 atgagcacgc agacccacgc accgagc
//
LOCUS       DATAIX010000078         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_3761, whole genome shotgun sequence.
ACCESSION   DATAIX010000078 DATAIX010000000
VERSION     DATAIX010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3761"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1903)
                     /locus_tag="VHM65_01230"
     CDS             complement(<1..1903)
                     /locus_tag="VHM65_01230"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX2704353.1"
                     /translation="MSNEEWPGERASGPGDGGAGGSGGAGGPGGSGGPGGSGGSSQRT
                     MVAAPRRGPLLPTLVILFALVVLLSGAASVWTEILWFDSVGFRSVFMTELGAKLLLGI
                     LAGLLAAGVVGSSLVLGYRTRPIYAPSTPEQESLDRYREAIEPLRRLATVAVPLMVGL
                     LVGVTASTQWQTYLLWLNRKPFGKKDPQFGLDIEWFVFTVPWLNAVVGLLSMVLIAGA
                     IAAVFTHYVYGGVQLTGSRHTMPAARLHLSALLAGLAVTRAAAYWLERYGLTTETSGL
                     MTGIQYTDATAVLPTKAILAVASLMCALMFLSVIWTQSWRLPIIGVVLLGVIAIVVGN
                     IYPSLIQSLKVKPSEKSLEEPYLARNIAATRDAFGIAGIDRIDYKAATTATPAQLRAD
                     AETVPGIRIVDPNVVAPTFKQKQLPKAYYQFPDTLDVDRYVVDGTLSDAVVAVRELDL
                     DGVPTGQRNWLNDHTVYTHGYGLVAAYGNRRGSEGEPVFFEGSTSSTGPLGEYQPRIY
                     FGEQSPSYSIVGAPAGASMREFDYPGDTASAKERSYTYTGSGGVGLGSLARRVAYGIK
                     YREANFLLSDAVNGSSRLLDYRTPKERVEKVAPWLTLDGNVYPAVVDGRVQWIIDGYT
                     TTAPPSRRAP"
BASE COUNT          327 a          668 c          681 g          274 t
ORIGIN      
        1 aaggagcgcg tcgagaaggt ggcgccgtgg tcgtgtaccc atcgatgatc cactggaccc
       61 gtccgtcgac gaccgccggg tagacgttgc cgtcgagggt cagccacggc gccaccttct
      121 cgacgcgctc cttcggggtg cggtagtcga gcagccgcga gctgccgttg acggcgtccg
      181 agagcaggaa gttggcctcg cggtacttga tgccgtaggc cacgcggcga gccagcgacc
      241 ccaacccaac cccaccgctc ccggtgtagg tgtagctgcg ctccttggcc gaggcggtgt
      301 caccgggata gtcgaactcg cgcatcgagg cccccgcagg cgccccgacg atcgagtatg
      361 acggggactg ctcgccgaag tagatccgcg gctggtactc cccgagcggg ccggtcgagg
      421 aggtcgaccc ctcgaagaag acgggctcac cctcagaccc gcgccggttg ccataggccg
      481 cgaccaaccc gtagccgtgc gtgtacacgg tgtggtcgtt gagccagttg cgctgcccgg
      541 tcgggacccc gtccaggtcg agctcgcgga ccgcgacgac agcatcgctg agggtgccat
      601 ccacgacgta acggtcgacg tcgagggtgt ccgggaactg gtagtaggcc ttgggcagct
      661 gcttctgctt gaacgtcggc gcgaccacat tggggtcgac gatccggatg cctgggaccg
      721 tctcggcgtc cgcgcgcagc tgcgccggcg tggccgtcgt cgcggccttg tagtcgatcc
      781 ggtcgatccc ggcgatcccg aacgcatctc gggtggccgc gatgttgcgg gcgagatagg
      841 gctcctcgag cgacttctcc gacggcttga ccttgagcga ctggatcagc gaggggtaga
      901 tgttgccgac gacgatcgcg atgacgccga gcaggacgac gccgatgatc ggcagccgcc
      961 aggactgcgt ccagatcacc gagaggaaca tcagcgcgca catgagcgag gcgacggcga
     1021 ggatcgcctt ggtcggcagg acggcggtgg catcggtgta ctggatgccg gtcatgagcc
     1081 cgctggtctc ggtggtcagc ccgtaccgct cgagccagta ggccgcggcc cgggtgactg
     1141 ccaggcccgc gagcagtgcg gacaggtgca ggcgagcggc gggcatcgta tgacgtgacc
     1201 ccgtgagctg cacaccgcca tacacgtagt gggtgaacac cgcggcgatc gccccagcga
     1261 tgaggaccat gctcagcagg ccgaccaccg cgttgagcca cggcacggtg aagacgaacc
     1321 actcgatgtc gagcccgaac tgcgggtcct tcttgccgaa cggcttgcgg ttgagccaca
     1381 gcaggtaggt ctgccactgg gtcgatgcgg tgaccccgac gagcagcccg accatgagcg
     1441 gcaccgccac ggtggcgagc ctgcgcaggg gctcgatggc ctcgcggtag cggtcgaggc
     1501 tctcctgctc gggcgtcgag ggggcgtaga tcgggcgcgt gcggtagccc aggacgagac
     1561 tggacccgac gacacctgcc gcaagcagtc cggcgaggat ccccagcagc agcttggcgc
     1621 ccagctcggt catgaacacc gagcggaagc ccaccgagtc gaaccacagg atctcggtcc
     1681 acaccgacgc ggctcccgac aggagcacca cgagggcgaa caggatcacc agggtcggca
     1741 gcagcggccc gcgccggggt gcggccacca tcgtccgctg cgacgagccg cccgaaccac
     1801 ccgggccgcc cgaaccaccg gggccgccgg cgccacccga accgcccgcc ccgccatcgc
     1861 cagggcccga agcccgctcg ccgggccatt cctcattgct cacgtcgtca acccacttgt
     1921 cgcctggcgc acggccggtc accgcggctc
//
LOCUS       DATAIX010000079         2226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_33928, whole genome shotgun sequence.
ACCESSION   DATAIX010000079 DATAIX010000000
VERSION     DATAIX010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33928"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..521
                     /locus_tag="VHM65_01235"
     CDS             123..521
                     /locus_tag="VHM65_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2704354.1"
                     /translation="MLTSSPVTTMLPARDVDRAREFYEKRLGLTPLGPRPDGSFHFAA
                     GGTTLALLPKPDGTKAEHTAVSFEVHNLGAVVIALQEAGVVFEDYDLPGLETVDHVCD
                     LGFERAAWFLDTEGNILCVHESLDSGASPG"
     gene            complement(547..1095)
                     /locus_tag="VHM65_01240"
     CDS             complement(547..1095)
                     /locus_tag="VHM65_01240"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX2704355.1"
                     /translation="MASARGSLSWLPLGEHLDLVGSPVARAAAQAPSAHVATIDATLA
                     DTAAFCAAYEVSLDHSANCVIVAARRGEVTRYAAVMVLATMRADVNGVVRRHLDARRI
                     SFAPMEDAMRLTGMEFGGITPVGLGPDWPVLVDRAVAAAGEVVIGSGVRTSKLLLDGH
                     DLAGLPGAHVLDLSLADASPRS"
     gene            1152..2090
                     /locus_tag="VHM65_01245"
     CDS             1152..2090
                     /locus_tag="VHM65_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX2704356.1"
                     /translation="MSVEGGTKAIMAALAANLGIAVTKFVAFLLSGSSSMLAEAVHSV
                     ADSGNQGLLLLGGKRARRDATPVHPFGYGRERYFWAFVVSIVLFMVGGLFALYEAFHK
                     WNHPEPITTWQWLPIVVLGVAIALESMSLRTAVREADKVRGSRSWGDFIRSAKAPELP
                     VILLEDLAALLGLVFALFGVSMTLVTANGRWDAVGTAMIGLLLVVVAVVLAVEMKSLL
                     LGEGATTEDVRTIEGALTGSPGIERIIHMKTLHLGPEELLVAAKVAVPFADTAADIAH
                     TIDRAETAVRTALPALTVLMYVEPDLDRSQSPSPLD"
BASE COUNT          327 a          805 c          757 g          337 t
ORIGIN      
        1 attgtagggc gacgtcgccc agacccgtcc cacgacgtcg ccctacaatt gccggcgtcg
       61 ggcacccgcc cgctgacctt cgtgccggcg atcggccgga cagctcgacc gaaggagtgg
      121 acatgctcac cagctcaccg gtcacgacca tgctccccgc acgcgatgtc gaccgcgccc
      181 gcgagttcta cgagaagcgc ctcggcctga ctcccttggg cccccgcccg gacggaagct
      241 tccacttcgc cgccgggggg accacgctgg ctctgctgcc caagcccgac gggacgaaag
      301 cggagcacac cgccgtcagc ttcgaggtgc acaacctcgg ggcggtggtc atcgccctgc
      361 aggaagccgg ggtggtcttc gaggactacg acctcccggg actggaaacg gtcgaccacg
      421 tctgcgacct cggtttcgag cgcgccgcat ggttcctgga caccgagggc aacatcctgt
      481 gcgtgcacga gtccctcgac agcggagcca gccccggctg agcgtcagct cacgaccggc
      541 acagcgctag ctccggggtg aggcgtcggc caacgagagg tcgagcacgt gcgcgccggg
      601 gagcccggca aggtcgtggc cgtcgagcag cagcttgctc gtccggaccc cgctgccgat
      661 cacgacctca ccggccgcgg cgacggcgcg gtcaacgagc accggccagt cgggcccgag
      721 cccgaccggg gtgatcccac cgaactccat gcccgtcagc cgcatcgcgt cctccatcgg
      781 cgcgaaggag atcctgcgcg cgtcgaggtg gcgccgcacg acaccgttga cgtccgcccg
      841 catcgtggcc aggaccatca ccgcggcgta gcgggtcacc tcgccgcggc gcgcggcgac
      901 gatgacgcag ttggccgagt ggtcgaggga gacctcatag gccgcgcaga aggccgcggt
      961 gtcggcgaga gtggcgtcga tggtcgcgac gtgcgccgac ggcgcctgcg ctgcggcccg
     1021 ggcgacgggg gacccgacga ggtccaggtg ctcccccagg ggcagccacg acagtgaccc
     1081 acgggcgctc gccataccgg gactctagcc gcgacgatcg ctgccgcgac gacctcggta
     1141 ggattcgcga catgtcggtt gagggcggga ccaaggcgat catggctgcg ctcgccgcca
     1201 acctcgggat tgcggtgacc aagttcgtgg cgttcctgct gtccgggtcg agctcgatgc
     1261 tcgccgaggc cgtgcactcg gtcgccgact cgggcaacca gggcctgctc ctgctcggtg
     1321 gcaagcgggc ccggcgtgac gccaccccgg tgcacccgtt cggctacggc cgggagcgct
     1381 acttctgggc ctttgtcgtc tccatcgtgc tgttcatggt cggtggcctc ttcgcgctct
     1441 acgaggcctt tcacaagtgg aaccacccgg agccgatcac gacgtggcag tggcttccga
     1501 tcgtggttct cggggtcgcg atcgccctgg agtcgatgtc cttgcggacc gcggtccggg
     1561 aggccgacaa ggtgcgcggg tcgcggtcct ggggggactt catccgcagt gccaaggcac
     1621 ccgagctgcc cgtcatcctg ctcgaggacc tcgccgcgct gctcggtctg gtcttcgccc
     1681 tcttcggcgt gtcgatgacg ctcgtcaccg ccaacggccg atgggatgcc gtcggcaccg
     1741 ccatgatcgg cctgctcctc gtcgtcgtcg ccgtcgtcct cgcggtggag atgaagtcgc
     1801 tgctcctcgg cgagggggcg accaccgagg atgtgcgcac gatcgagggg gcgctgacgg
     1861 gcagccccgg gatcgagcgc atcatccaca tgaagacgct gcacctgggt cccgaggagc
     1921 tcctcgtcgc ggccaaggtg gccgtgccgt tcgcagacac cgccgcggac atcgcccaca
     1981 cgatcgaccg cgccgagacg gcggtgcgca ccgccctgcc cgcgctcacc gtcctgatgt
     2041 acgtcgagcc ggatctcgac cggtcccaga gcccttcacc gctagactga gccctgaacc
     2101 ggcgtgtgtg atatcccgcg accaccgcgc ccagaggggc tacgccgggt cgcacgcctc
     2161 gccgtgctcc ccacctgccc tggtatggcg cgcccgcgcg ccataccgtt catgaattcc
     2221 tggagg
//
LOCUS       DATAIX010000080         2040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_63680, whole genome shotgun sequence.
ACCESSION   DATAIX010000080 DATAIX010000000
VERSION     DATAIX010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63680"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VHM65_01250"
     CDS             <1..222
                     /locus_tag="VHM65_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704357.1"
                     /translation="RLDVSFTSHALSVPSVLATCRHCFDRVPDSLVLAIRGYEWELSI
                     GLTPGAHANLAAATAYVRSARSVPIPEPA"
     gene            235..735
                     /locus_tag="VHM65_01255"
     CDS             235..735
                     /locus_tag="VHM65_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX2704358.1"
                     /translation="MTEKSFDRIATTIYQAPVGQYIGREGAEVLARHAGDEVNLQDKE
                     VLFRKGEHATTFYILTQGRLALLHEATKNRPAAILHVLEQGDLVGELSFIDGSKHTVS
                     CCALGEASVLSFNVRDLTPLIDENPRVLYDFMRAVIMRVHHTSASISRQRQELADYIS
                     TGGRRF"
     gene            complement(705..1595)
                     /locus_tag="VHM65_01260"
     CDS             complement(705..1595)
                     /locus_tag="VHM65_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704359.1"
                     /translation="MDTRSTVTRDDRVLTYTRGVSLFVAPFLLVAFVILYLFPGETQR
                     LFAWTIRPTMTSMTLASAYLGGFYFFLRVLREQRWAAMKTGFLSVALFASLLGVATVI
                     HWDRFNHGHVAFWLWAGLYFAAPLLVVGAWLANRRYAAPAGVGEQRVGGTARWVAGLV
                     GLVALGTGLAMFLAPMRMIGVWPWLLTPLTCRVVGAIFCLGSAGLGVLVDPRWVAVKL
                     MLEVEVLMVALILVAAVRARDEFFTDRPLTWLLIGGFLAILAGSAYLWFFEQGRARLG
                     AAQCARSTSAPQNLRPPVEM"
     gene            complement(1772..>2040)
                     /locus_tag="VHM65_01265"
     CDS             complement(1772..>2040)
                     /locus_tag="VHM65_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2704360.1"
                     /translation="LGGGFLAQPGRRTVCGTLTGAGLSRLWSRHRAHRFFSHVRWSSE
                     DVTAVLTRLVVDRLVPAGDAVVVAVDDTLFKRNLVSYLRRVCNK"
BASE COUNT          380 a          707 c          687 g          266 t
ORIGIN      
        1 cggctcgacg tgagcttcac ctcacacgcc ctgtcggtgc cgtcggtcct cgccacctgc
       61 cgacactgct tcgaccgcgt ccccgactcc ctcgtcctgg ccatccgcgg gtacgaatgg
      121 gagctgtcca tcggcctcac accgggggcc cacgcaaacc tggcagctgc gacggcatac
      181 gtccgatctg cccgtagtgt ccccatcccc gaaccggcct gactggagcg cgccatgacc
      241 gagaagtcct tcgaccgcat cgcgacgacg atctaccaag ccccggtcgg ccagtacatc
      301 ggccgggagg gtgcggaggt cctcgcccgg cacgccggtg acgaggtgaa cctgcaggac
      361 aaggaggtgc tcttccgcaa gggtgagcac gcgacgacgt tctacatcct cacccagggc
      421 cggctggccc tcctgcatga ggcgacgaag aacaggcccg cggccatcct tcacgtgctg
      481 gagcagggcg acctggtcgg ggagctgagc ttcatcgacg ggagcaagca cacggtctcc
      541 tgctgcgcgc tcggcgaggc ctccgtgctg tccttcaacg tccgcgacct cacccccctc
      601 atcgacgaga acccgcgggt gctctacgac ttcatgcgcg ccgtcatcat gcgggtccac
      661 cacacgtcgg cgtcgatcag ccgccagcgc caggagctgg ccgactacat ctcgaccggg
      721 ggccgcaggt tctgaggcgc cgacgttgag cgagcgcatt gagcggcgcc gagccgggcg
      781 cgaccctgct cgaagaacca gaggtatgcc gacccggcga ggatcgcgag gaagccgccg
      841 atcaggagcc aggtgagagg ccggtccgtg aagaactcgt cgcgtgcgcg gaccgccgcg
      901 acgaggatca gggcgaccat gagcacctcg acctcgagca tgagcttgac ggcgacccac
      961 cgcgggtcga caaggacgcc gagcccggcg ctgccgagac agaagatcgc gccgacgact
     1021 cggcaggtca agggcgtgag cagccacggc cagacgccga tcatccgcat gggcgcgagg
     1081 aacatggcca ggcccgtccc gagcgcgacg agcccgacaa gcccggcgac ccaccgggcg
     1141 gtgccgccga ctcgctgctc gccgacgccg gccggcgcgg cataccgccg gttggccagc
     1201 caggcgccga cgacgagaag gggtgcggcg aagtagaggc ccgcccacag ccagaaggcg
     1261 acgtgtccgt ggttgaaccg gtcccagtgg atgaccgtcg cgacgccgag cagcgacgcg
     1321 aacagcgcga cggagaggaa ccccgtcttc atcgcggccc agcgctgctc ccgcagcacc
     1381 cggaggaaga agtagaaacc gccgaggtat gccgacgcca gtgtcatcga ggtcatcgtg
     1441 ggtcggatgg tccacgcgaa gagccgttgg gtctcaccgg ggaagaggta gaggatgacg
     1501 aaggcgacga ggaggaaggg cgcgacgaag agcgatacgc cccgggtgta ggtgaggacc
     1561 cggtcgtcgc gagtcaccgt ggatcgtgtg tccatatccg gacctctgtc gcgaagatgt
     1621 gaccttggtc gatactagac acccggtgga cccttcgaca gggggagacg atgtagattg
     1681 ccgacctcct cgcgccaggg caggtgctcc tgcggctggc ggtggccggc ttcggagtgg
     1741 tcgtgcctcc accggggtga ccccggcgcg cctacttatt gcaaactctt cgcaagtagg
     1801 aaacgaggtt gcgcttgaac agggtgtcgt ccaccgcgac caccaccgca tcaccggccg
     1861 gcaccaaacg gtcgacgacc aaccgggtca gcaccgcggt cacgtcctcg gatgaccagc
     1921 ggacgtgcga gaagaaccga tgcgcccggt gacgagacca cagccgcgac aaccccgccc
     1981 ccgtcagcgt gccgcacacc gtgcgacggc ccggctgcgc caagaacccg ccgcccagcg
//
LOCUS       DATAIX010000081         1722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_153273, whole genome shotgun sequence.
ACCESSION   DATAIX010000081 DATAIX010000000
VERSION     DATAIX010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153273"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..322)
                     /locus_tag="VHM65_01270"
                     /pseudo
     CDS             complement(1..322)
                     /locus_tag="VHM65_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025991973.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(566..1336)
                     /locus_tag="VHM65_01275"
     CDS             complement(566..1336)
                     /locus_tag="VHM65_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2704361.1"
                     /translation="MSVVITIGQLARYAGVSTKTIRVYHEKGLLAEPDRDASGYRRYT
                     AQDAVELIKIRTLAEAGVPLARIRALKAAPDDAFHHALTEIDDDLTARIRSLRHTQHR
                     LREFASGHTRLLPTEVDHQLDQLGELGFSERWVSLQRDLWILVYATHPDTAPELFSDQ
                     AQALTDPTLRQIYLDYDRAHDLDPGDPLLTDLARRMVDATTERYGAGDLPGQTTGSEI
                     PNLIQGAVNALSPAWQRLDTLVRAELQRAAAGPHAHRT"
     gene            1386..1673
                     /locus_tag="VHM65_01280"
     CDS             1386..1673
                     /locus_tag="VHM65_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704362.1"
                     /translation="MTSVSPDRETLRALADEGNELALDRLADLADARADLAELSDLLD
                     EGCMHAGHLLTRRAVATGDLLELQRIWDAGCDEAGDELQRLLAAPTSPGEE"
BASE COUNT          257 a          528 c          649 g          288 t
ORIGIN      
        1 atgttgcagg cctcggccaa caggacggcg cagatgctga cgccgaggtc ctcggcgcgt
       61 gctgatccct cggacacgtg cgtgaactca tcaaggaaac ccgtccagcc ggcgacgtcc
      121 agcaggacat cgggcaggtc gatgcgcggc aaccgctctt ggacgccgtc tcggagcgca
      181 agcaggctgg ccggttcctc cagcttgtcc agggggtgag gacggggcgg tcgcgcccgt
      241 cgacagtctc gatgcgaact gccgtgttgt ctggccggcg ttcggcgacg gcccggtatg
      301 cgccgtccaa atcggctgtg agcgtgtcga gctccgccgt caaacgcacc cggcaccgcg
      361 actggcccca accctcacga tccatcccaa gacgccgtgg tgacctaatt gaacggtact
      421 aagcctaggc ttggcccccc gaggccatga tcgccctggc catgctcaac cactgcggtt
      481 gcaccaccct cgcgctgcga actcgtcata gatcccgggc cgcggggttg ggtatgacga
      541 tgagctcgcc ccttcgcgca gaacctcagg tccggtgcgc atggggtcca gccgcagcgc
      601 gttggagctc ggcgcggacc agggtgtcga gtcgctgcca ggcgggggac aaggcgttga
      661 cggcgccctg gatcaggttg gggatctccg agccggtcgt ctgtccgggg agatcgccgg
      721 ctccgtaccg ctctgtggtg gcgtcgacca tgcgtcgggc gaggtcggtg aggagggggt
      781 ctcccgggtc gaggtcgtgg gcgcggtcgt agtcgaggta gatctgccgg agggtggggt
      841 cggtcagtgc ctgggcctgg tcgctgaaga gctcgggcgc ggtgtccggg tgggtggcgt
      901 acacgaggat ccacaggtcg cgctgcaacg agacccatcg ttcgctgaaa ccgagctcgc
      961 cgagctgatc gagctggtgg tcgacctcgg tgggaagcag ccgggtgtgg ccggaggcga
     1021 actcccgcag ccggtgctgg gtatggcgca ggctgcggat gcgggcggtg aggtcgtcgt
     1081 cgatctcggt gagggcatgg tggaaggcgt cgtccggtgc cgccttgagg gcacggattc
     1141 tggcgagggg gactccggcc tcggccaggg tgcggatctt gatgagctcg acggcgtcct
     1201 gggcggtata gcgccggtag ccggaggcgt cacggtcggg ttcggccagc agtccctttt
     1261 cgtggtagac gcggatggtc ttggtcgaca cgccggcgta ccgggcgagc tggccgatgg
     1321 tgatcacaac gctcatgctc tcgtacttga ccttgcccta ggggcaaggt tgcagcatgt
     1381 gtggcatgac gagcgtgagc cccgatcggg agaccctgcg agcactggcc gacgagggta
     1441 acgagctggc cctggatcgg ctggctgacc tcgcagatgc gcgcgcggac ctcgccgagc
     1501 tgagcgatct gctggacgaa ggctgcatgc atgcggggca cctgctgacc cgccgcgcgg
     1561 tcgccacggg tgacctgctg gagctgcagc ggatctggga cgcagggtgc gacgaggccg
     1621 gcgatgagct ccaacggctc ctcgccgccc cgacaagccc cggcgaggag tgagcacggc
     1681 ggtccgcttg cggctagcca caccggagtg gcatggtcgg tc
//
LOCUS       DATAIX010000082         3934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_63981, whole genome shotgun sequence.
ACCESSION   DATAIX010000082 DATAIX010000000
VERSION     DATAIX010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63981"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="VHM65_01285"
     CDS             complement(<1..1248)
                     /locus_tag="VHM65_01285"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX2704363.1"
                     /translation="MSVLETIGGPDDLKRLAPEQIPALALEIRDFLVASVSRTGGHLG
                     PNLGVVELTIALHRVFDSPRDTIVFDTGHQAYVHKILTGRQDFSRLRQRGGLSGYPSR
                     TESEHDVVENSHASTALSWADGIATARRLRGERDRHTVAVIGDGALTGGMAWEAINNI
                     AADKDLPLVIVVNDNERSYAPTTGGLAEHLATLRTARSYERVLDWGKHALVKTPVVGG
                     AMYHSLHGMKKGIKDIVAPQGLFEDLGLKYLGPIDGHDEPAIESALRRARAYGGPVLV
                     HVLTKKGMGYRPARQNEDDRFHAVGVINPETGLPLETVGRSWTDDFSEELCLLGRERP
                     DIVALTAAMLIPVGLKEFADEFPGRVFDVGIAEQHAVTMAAGLAFGGLHPVVAVYATF
                     LNRAFDQLLMDCALHRAGVTFVLD"
     gene            1416..2792
                     /locus_tag="VHM65_01290"
     CDS             1416..2792
                     /locus_tag="VHM65_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX2704364.1"
                     /translation="MDDLSSLFHRAAQHAADYRASLAERSVVPLADVGTLRKALGGPL
                     PQRRSAPGDVLEQLVESAEPGLMSSAGPRFFGFVIGGSLPAATAADMVAVGWDQCAFN
                     AVLSPAAAVAEEVAGGWLKDLLGIPPGCSVGFVTGAQEANTVGLAAGRHHVLAKAGRH
                     VERDGLLGAPVVRVVAGVERHATIDRSLRLLGFGTACVEPVRADANGALDIADLDRVL
                     ASGPNGPMIVCLQAGNVNTGACDDLSAGCEVAHRHGAWVHVDGAFGLWAAASPTTRHL
                     VHGVERADSWGCDGHKWLNLPYDSGFALCAHPQEHAAAVSYTAAYLVGSGGAAPGSAD
                     LTLESSRRARGFAAWAALKELGREGVAELVERPCRLARRFAASLSAGGAEVVNDVVLN
                     QVLVRFGDDDRTERVITAVQREGTCWLGGTSWQGRRLMRISVSNWSTTEADVDRSVEA
                     ILRVAASA"
     gene            complement(2851..3246)
                     /locus_tag="VHM65_01295"
     CDS             complement(2851..3246)
                     /locus_tag="VHM65_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2704365.1"
                     /translation="MSRVVHFEIQADVVERAKAFYAAVFDWSFEDYGPLTGSTYWGVV
                     TGPPDQPGINGGLLQRPAPAPAVGRGTNAFVCTMEVEDYDATERRILDAGGQVALPKS
                     ALVGMAWQGYYLDTEGNTFGIHQPDPEAA"
     gene            complement(3341..>3934)
                     /locus_tag="VHM65_01300"
     CDS             complement(3341..>3934)
                     /locus_tag="VHM65_01300"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX2704366.1"
                     /translation="SRARLTYPASEGAPGKPALLNGSGDIQGGHGSDDSYHGTYDVGN
                     GPWAPRVATAVTGSRYRDAAGRPRQGRPRDALGRPLPYGASGVEPVPEEPLPPSQTLD
                     LARSLVHEGRPFAAHEVLEARWKAGPGEERDLWQGLAQVCVGLTHAARGHSVGAVRLL
                     DRGAARLEEYRAGGGCTYGLDLAAVVTCARERTATRR"
BASE COUNT          542 a         1381 c         1408 g          603 t
ORIGIN      
        1 gtcgaggacg aaggtgacgc cggcgcgatg cagcgcacag tccatgagca gctggtcgaa
       61 cgcgcggttg aggaaggtcg catacacggc gacgaccggg tgcagcccgc caaaagcgag
      121 cccagcagcc atcgtgaccg catgctgctc ggcgatgccg acgtcgaaga cccggcccgg
      181 gaactcgtcg gcaaactcct tgagtcccac cgggatgagc atggccgcgg tcaacgcgac
      241 gatgtccggc cgctcccgcc cgagcaggca cagttcctcg ctgaagtcgt cggtccagct
      301 gcgcccgacg gtctccaagg gcaggccggt ctccgggttg atgaccccga ccgcgtggaa
      361 gcggtcgtcc tcgttctggc gggccggccg gtagcccatg cctttcttcg tcagcacgtg
      421 aaccagcacc ggaccgccat acgccctggc tctgcgcagc gcagactcga tggcgggctc
      481 gtcgtgcccg tcgatgggac cgaggtactt caggccgaga tcctcgaaga gcccctgcgg
      541 ggcgacgatg tccttgatcc ccttcttcat gccgtgcagc gagtggtaca tcgcgccgcc
      601 gaccacgggg gtcttgacca gcgcgtgctt gccccagtcg aggacacgct cgtagctgcg
      661 cgcggtccgc agggtcgcca ggtgctcggc gagcccgccg gtggtggggg cataggagcg
      721 ctcgttgtcg ttgacgacga tgacaagcgg taggtccttg tcggcggcga tgttgttgat
      781 cgcctcccac gccatcccgc cggtgagcgc cccgtcgccg atgaccgcga cggtatgacg
      841 gtcacgctcg ccccgaagtc ggcgcgcggt ggcgatcccg tcggcccagg acagcgccgt
      901 ggacgcatgc gagttctcga cgacgtcgtg ctcggactcg gtgcggctcg ggtagcccga
      961 cagcccgccg cgctgacgca gccgcgagaa gtcctgacgg cccgtgagga tcttgtgcac
     1021 gtaggcctgg tgaccggtgt cgaacacgat cgtgtcccgc ggggagtcga agacccggtg
     1081 cagcgcgatc gtcagctcga caacccccag gttggggccg agatgtccgc cggtccgcga
     1141 cacggacgcg acgaggaagt cgcggatctc cagcgccagg gcggggatct gctcgggagc
     1201 aagacgtttg aggtcgtcgg ggccgccgat cgtctcgagc acactcacgt tgggtcccgt
     1261 tccttcctga tgctcaacgg agcgagtcta gttgagccgc gtccgcagac ccccagtggc
     1321 acggcgtcgc gctgggtgcg ggacatgtcc gccagggtgc ggacctcacc ccgggtttcc
     1381 accctcgacc ggtaacgggc gtagcgtgtc ctgtcgtgga cgacttgagc tcgttgtttc
     1441 accgggccgc acagcacgcg gccgactacc gtgcgagcct tgcggagcgc tccgtcgttc
     1501 ccctggccga cgtcggcacg ctgcggaagg ccctgggggg tcccctcccg cagaggcggt
     1561 ccgcaccggg cgacgtgctc gagcagctcg tcgagtccgc cgagcccggc ctcatgtcca
     1621 gcgccggccc gaggttcttc gggttcgtca tcgggggcag cctcccggct gcgacggcgg
     1681 ccgacatggt cgccgtgggc tgggaccagt gtgccttcaa cgcggtgctc tcccctgcgg
     1741 cggccgtggc tgaagaggtt gcgggaggct ggctgaagga ccttctggga atcccgccgg
     1801 ggtgctccgt cggcttcgtc accggcgccc aggaggccaa caccgtgggt ctggccgccg
     1861 ggcgtcacca cgtccttgcc aaggcaggcc ggcacgtgga gcgcgacggt ctgctcggcg
     1921 ctccggttgt ccgggtcgtg gccggtgtcg agcggcacgc caccatcgac cggtccctgc
     1981 ggctgctcgg cttcggcacc gcgtgtgtgg agcccgtccg ggccgacgcc aacggcgcgc
     2041 tggatatcgc cgatctcgac cgcgtgctag cgagcggccc caacgggccg atgatcgtat
     2101 gcctgcaggc cggcaacgtg aacaccggcg cgtgcgacga cctgtcggcc ggctgtgagg
     2161 tcgctcaccg gcacggcgcg tgggtgcacg tggacggcgc attcggcttg tgggccgccg
     2221 ccagcccgac cacgcgacac ctcgtgcacg gcgtggagcg ggccgactcg tggggctgcg
     2281 acgggcacaa gtggctgaac ctgccctacg acagcgggtt cgcactctgc gcccatccgc
     2341 aggagcatgc tgccgctgtg tcctacacag cggcgtacct cgtggggtcc ggcggtgcgg
     2401 ccccgggatc tgcggacctc acgttggagt cctcgcgcag ggcgcgcggg tttgccgcgt
     2461 gggcagccct caaggagctg ggccgggagg gtgtcgctga gctggtcgag cggccgtgcc
     2521 gactggctcg tcggttcgct gcctccttgt cagccggcgg cgccgaggtg gtcaatgacg
     2581 tcgtgctcaa ccaggtcctg gtgcgattcg gcgacgacga ccggaccgag cgggtcatca
     2641 ccgccgtcca gcgcgaggga acctgctggt tggggggcac ctcctggcag gggcgacgcc
     2701 tgatgcggat ctcggtgtcc aactggtcca ccacggaagc ggacgtggac cgctcggtcg
     2761 aggcgatcct gcgcgtcgcg gcctcggcgt gagcgacgtc cgcccatgcc gccgtccgcg
     2821 cgctgccgat gagcaggccg ggggggccgg ctacgccgcc tcgggatccg gctggtggat
     2881 gccgaacgtg ttgccctcgg tgtccaggta gtagccctgc cacgccatgc ccaccagcgc
     2941 gctcttgggc agggcgacct gcccgcccgc atcgaggatg cggcgctcgg tcgcgtcgta
     3001 gtcctcgacc tccatggtgc agacgaacgc gttcgtgccc cggcccacgg caggcgccgg
     3061 cgcggggcgc tgcagcaggc ccccgttgat gcccggctgg tccggcgggc cggtcacgac
     3121 gccccagtag gtcgagccgg tgagcgggcc gtagtcctcg aacgaccagt cgaacacggc
     3181 ggcatagaac gccttcgccc gctcgacgac gtccgcctgg atctcgaaat gcaccacacg
     3241 tgacatgccg gcctcctcgg gttggctgga ggcgccagtc tggcactgac caccggccac
     3301 cggctactgc ccgtagggct tcccggcgcc cggctactcc tcaacgacgg gtggccgtac
     3361 gctcgcgcgc acaggtcacc accgccgcca ggtcgagccc gtacgtgcag ccgccgccgg
     3421 cgcggtactc ttcgagccgg gccgcgcccc ggtcgaggag ccggacagct ccgacgctgt
     3481 gaccacgggc ggcatgggtg aggccgacac agacctgggc gagaccctgc cacaggtcgc
     3541 gttcctcgcc cggaccggcc ttccagcggg cctcaaggac ctcgtgcgcc gcaaagggcc
     3601 tgccctcgtg cacgaggctc cgggccaggt ccagggtctg cgacggcggc agaggctcct
     3661 ccggcacggg ctcgacgccg ctggccccgt agggcagggg ccggccgaga gcatcacgtg
     3721 gccgcccctg acgtgggcga ccggccgcgt cgcggtaacg actgccggtc acggctgtcg
     3781 ccaccctggg tgcccatgga ccattcccga cgtcatacgt cccatggtac gagtcgtcac
     3841 tgccgtgacc gccctggatg tccccggacc cgttcagcag cgcgggctta ccgggcgcgc
     3901 cctcagaggc ggggtacgta agccttgccc tgga
//
LOCUS       DATAIX010000083         3336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_4080, whole genome shotgun sequence.
ACCESSION   DATAIX010000083 DATAIX010000000
VERSION     DATAIX010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4080"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1426
                     /gene="efeB"
                     /locus_tag="VHM65_01305"
     CDS             128..1426
                     /gene="efeB"
                     /locus_tag="VHM65_01305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HEX2704367.1"
                     /translation="MSAKPDISRRRMLGLTAGAVVGTGVLAASQAHTQGPAASPTGGV
                     PPPGGTQGPDPTALQYPFRGVRQAGIITPAQDRMHFVALDVVTSDRAALSDLLERWTR
                     AAERMTAGAEAAPGGLVGGGPNAAPGDTGEAYDLPPAGLTITVGYGPSLFDGRFGLAT
                     RRPEPLRDLPAFAGDDLDPARSGGDICVQACANDPQVAVHAIRNLVRMGFGIVSVRWS
                     QLGFGRTSSTTRSQLTPRNMFGFKDGTANITADESAELAEHVWVGAQDGGPGQQWLVG
                     GTYLVARRIRMQIEVWDRTPLGEQEQIFGRDKSRGAPLGGTAEFDEVDFGRRGTDGEL
                     AIPMAAHIRLAHRSNLDGVRILRRGYNFTDGTDGVGHLDAGLFFVAFCRDAHRQFVPM
                     QRALASADVLNEYIEHSGSAVFACPPGLAQGQDWGSQLLG"
     gene            complement(1428..2252)
                     /locus_tag="VHM65_01310"
     CDS             complement(1428..2252)
                     /locus_tag="VHM65_01310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX2704368.1"
                     /translation="MSQSSAGSPKAPARRAGRVIAYCGLAYLFSWAVWVPMMIQGQVS
                     RPGQGWPTHLLGLLGPALAALVVTAAVEGRPGVTALARRTLRWRGVGRWYWAVGAILL
                     VGAGAVAVLALTGAKVPSVEELSTYSGAPVTEPLLLLGYVLVVNGFGEEIGWRGFLAE
                     ELLTRHRVLPTAVVTALIWAGWHLPLFWVVESFRAMGWTSLGWLVGLVCGSIVLTWMY
                     HGSGRSILLVALWHTAYNVTSATQGTHGIPAAATSTAVMVAAITIAVVELRRRAGA"
     gene            2318..2962
                     /locus_tag="VHM65_01315"
     CDS             2318..2962
                     /locus_tag="VHM65_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2704369.1"
                     /translation="MNQSSGSPARSGSQRPVGRPPGASPSGIPDKLIAAAWSALLAGD
                     TPTVAGLCEAVPCTPPSLYHHFGSVHRLILAASEREFALFRERLAASVATAASPEDAI
                     RRRGRAYLDWGLANPGAYRVLFMSRGVPGSQQHGGVAEGGDGLSALVDDVRAAMAAGS
                     LGGGDPMVVALAHWAAVHGLTSLGVCSPDVPRGVLHAALEVLADALSRPAAGSA"
     gene            complement(2992..>3336)
                     /locus_tag="VHM65_01320"
     CDS             complement(2992..>3336)
                     /locus_tag="VHM65_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704370.1"
                     /translation="VPARDRVRASLLVYLDHIGERSTGWAAPFLHAGNDPDPAAEVRR
                     QARGASVGRLRALLQPSVAPRHDYALPGFFGFLDGACLEWVRRGCPEHDRHPLVEAAL
                     GALEGALGDWGG"
BASE COUNT          497 a         1197 c         1200 g          442 t
ORIGIN      
        1 ttcgggcacc ggctacgtcc tgtacacgga tctgagcaag gacgacatca aggcgctgac
       61 cgtcgcgctc gacgccgtga gcgaaccggt ctcacgggtc gccgcggtcg tcgacaaggg
      121 gtgagccgtg agcgccaagc ctgatatctc ccggcggcgg atgctcggcc tgaccgcggg
      181 cgccgtggtc ggtaccggcg tgctggccgc ctcgcaagcc cacacgcagg ggcctgcggc
      241 ctcgccaacc gggggagtgc caccccctgg cgggacccag ggccccgacc cgacggctct
      301 gcagtacccg ttccgcgggg tcaggcaggc cgggatcatc acgccggccc aggaccgcat
      361 gcacttcgtg gcgctggatg tcgtcacctc ggaccgcgcg gccctgagcg acctgctcga
      421 gcggtggacc cgtgccgccg agcggatgac ggccggtgcc gaggccgctc ccgggggtct
      481 tgtcggcggt gggcccaacg ccgctcccgg ggacacgggc gaggcatacg acctgccgcc
      541 ggcaggcctg acgatcacgg tcggctacgg gccctcgctc ttcgacgggc ggttcggcct
      601 ggccacgcgc aggccagagc cgctgcgcga cctgcctgcc ttcgccggtg acgacctcga
      661 cccggcgcgg tccgggggcg acatttgtgt ccaggcctgt gccaacgacc ctcaggtggc
      721 cgtgcacgcc atacgcaacc tcgtccgaat gggcttcggc atcgtgtcgg tgagatggtc
      781 ccagctgggg ttcgggcgga cgtcgtcgac gacgcggtcc cagctgacgc cgaggaacat
      841 gttcgggttc aaggacggca ccgccaatat cacggccgat gagagtgccg agctggccga
      901 gcatgtctgg gtcggcgcgc aggatggcgg cccgggtcag cagtggctcg tcggtggcac
      961 ctacctcgtc gcccggcgga tccggatgca gatcgaggtg tgggaccgca cgccgctggg
     1021 ggagcaggag cagatcttcg ggcgggacaa gtcccggggc gcgcccctgg gcgggacggc
     1081 cgagttcgac gaggtcgact tcgggcgacg agggacggac ggcgagctcg ccatacccat
     1141 ggccgcgcac atccggctcg cccaccggag caaccttgac ggcgtccgga tcctgcggcg
     1201 cgggtacaac ttcaccgacg gcaccgacgg ggtgggacat ctcgacgcgg ggctgttctt
     1261 cgtggcgttc tgccgcgacg cccaccgcca gttcgtgccc atgcagcggg cgctcgcctc
     1321 ggcggacgtg ctcaacgagt acatcgagca cagcgggtcg gcggtcttcg cctgcccacc
     1381 aggtctcgcg caggggcagg actgggggag ccagctgctc ggctaggcta ggcgccggcc
     1441 cgccgcctca gctcgaccac cgcgatggtg atcgcagcca ccatcacggc ggttgaggtg
     1501 gccgcagccg ggatgccgtg ggttccctgt gtcgcgctgg tgacgttgta tgccgtgtgc
     1561 cacagggcga cgaggaggat actgcgcccg ctgccgtggt acatccaggt gagcacgatc
     1621 gatccgcaca ccaggccgac gagccagccg agcgaggtcc accccatcgc ccgaaagctc
     1681 tcgacgaccc agaacaacgg caggtgccag cccgcccaga tcagcgcggt gacgacggcg
     1741 gtggggagca ctcgatgccg tgtcagcagc tcctccgcga ggaagccgcg ccagccgatc
     1801 tcctcaccga acccgttgac gaccaggacg tagccgagca gcaggagcgg ctcggtgacc
     1861 ggcgccccgc tgtacgtcga cagctcctcg acgctgggca ccttcgcgcc ggtcagcgca
     1921 agcaccgcca ccgcgcccgc gccgacgagc aggatcgccc ctaccgccca gtaccaccgt
     1981 ccgacgcctc gccagcgcag ggtccggcgg gccaatgcgg tcacaccggg ccgcccctcc
     2041 acggccgcgg tgacgacgag ggccgccagt gccggaccga ggagcccgag caggtgagtc
     2101 ggccagccct gaccaggacg cgagacctga ccctggatca tcatgggcac ccagaccgcc
     2161 cacgagaaga ggtacgcgag accgcagtag gcgatcaccc gcccggccct gcgtgccggg
     2221 gccttggggg agccggcgga cgactgcgac atgatgtcaa cccaacctgt gttatgtagt
     2281 gccactataa tactctcatg cgagccggtc gggaccgatg aaccagtcga gcggctcacc
     2341 cgcgcgctct ggcagtcagc ggccagttgg acggccgccg ggcgcaagcc cgagtggcat
     2401 tcccgacaag ctcatcgcgg ccgcatggtc ggctctgctc gccggagaca cccccacggt
     2461 cgccgggctg tgcgaggccg tgccctgcac gccgccctcg ctttaccacc acttcggctc
     2521 cgtgcaccgg ctcatcctgg ccgcgtccga gcgggagttc gcactcttca gggagcgcct
     2581 ggccgcctcg gtcgccaccg cggcgagccc ggaggacgcc attcgacgga gaggacgggc
     2641 ctacctggac tggggtctgg ccaacccggg cgcctaccgg gtgctcttca tgagccgggg
     2701 cgtcccgggg agccagcagc acggcggcgt cgccgaggga ggcgacggcc tcagcgcgct
     2761 tgtcgacgac gtgagggcgg cgatggccgc cggcagcctg ggaggaggcg acccgatggt
     2821 ggtcgccctc gcgcactggg cggccgttca tggcctgacc tcgctcgggg tgtgcagccc
     2881 ggacgtgcct cggggggtgc ttcacgccgc gctggaggtt cttgccgacg cgctgagccg
     2941 gcctgcggca ggcagcgcct agtggacggg ccgggcgtgc cactgctgcg gttagccgcc
     3001 ccagtcgccg agcgcgccct cgagtgcccc caaggcggcc tcgaccagcg gatgacggtc
     3061 gtgctcgggg cacccgcgcc tcacccactc cagacacgcc ccgtcgagga agccgaagaa
     3121 gcccggcaac gcgtagtcgt gccgcggcgc gacggagggc tggagcaggg ccctgagccg
     3181 cccgacagac gccccgcgtg cctggcgccg cacctccgcc gctgggtccg ggtcgttgcc
     3241 cgcgtgaagg aagggcgccg cccatccggt cgagcgctca ccgatgtggt cgaggtagac
     3301 cagcagggac gcccggacgc gatcccgggc cgggac
//
LOCUS       DATAIX010000084         1946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_64122, whole genome shotgun sequence.
ACCESSION   DATAIX010000084 DATAIX010000000
VERSION     DATAIX010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64122"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..1150)
                     /gene="ftsX"
                     /locus_tag="VHM65_01325"
     CDS             complement(230..1150)
                     /gene="ftsX"
                     /locus_tag="VHM65_01325"
                     /inference="COORDINATES: protein motif:HMM:NF038346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX2704371.1"
                     /translation="MSEPMKPGFLLSETANGMRRNLSMAVSVVLVTMISMYLLGLGML
                     AQRQADTMKDYWYERVQVSIFLCVDKSLYPNCAGKATTEGQRDQIRAQLEQMRPPVKD
                     VFYESQAEAFARFKAQFKNSPIGEQATERAFADSFRVSLSDPSKFDVIASSFQGAPGV
                     GRVQDQKELLNRFFSFINALSWSAIVLASLMVLCAVLLISTTIRQTAFSRRRETGIMR
                     LVGASNLTIQLPFVLETMISSLLGAGLAVGMLWATVRYGVSGYLSSALLDTAFIGVAD
                     VSAIAPYVVGGVALLAIVTSAVTLRRYLGV"
     gene            complement(1143..>1946)
                     /gene="ftsE"
                     /locus_tag="VHM65_01330"
     CDS             complement(1143..>1946)
                     /gene="ftsE"
                     /locus_tag="VHM65_01330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX2704372.1"
                     /translation="ISFDQVSKVYPRTTRPALDRVSVEIERGEFVFVVGKSGSGKSTM
                     LRLILKEENATSGTIHVAGRDLGRLAYRKVPRLRREIGTVFQDFRLLPNKTVSANVAF
                     ALQVLGKPRSVIRQTVPEALAMVGLEGMEKRFPHELSGGEQQRVAIARAVVNKPAILL
                     CDEPTGNLDPTTSLDIVTLLQRINRGGTTVLMATHDHEMVNTRRRRVVELDEGVLVRD
                     QSDGRYGGQLATSAGSVEPPAAGAGEPGPAGPGQQMQPEHPGASTEAGE"
BASE COUNT          322 a          664 c          660 g          300 t
ORIGIN      
        1 gttcttgtcc ccacgtgggt cagccccgga ggcggctccc tggacgagga ccagggagca
       61 ggccacgact gcggcaagac cggccacgcg gcgtcgtgct gcgttcatga acacacctct
      121 cccatggaca ccagtaggca cactggcatc accatagccc gtcggccatc ccccgcggtg
      181 gtctggaccc ccagacttct caggccgcca cgaggatcac ctgataccgc tacaccccaa
      241 gatagcggcg caaggtcacg gctgaggtca caatggcaag aagtgccacc ccgccgacga
      301 cgtatggcgc gatcgccgac acgtcggcta ccccgatgaa cgccgtgtcg agcagggcgc
      361 tactgaggta gcccgagacg ccataccgca cggttgccca gagcatcccg acggcgagcc
      421 ccgccccgag cagcgaggag atcatcgtct ccagcacgaa ggggagctgg atcgtgaggt
      481 tcgaggcgcc gacgagacgc atgatgccgg tctcccggcg ccggctgaac gccgtctggc
      541 ggatggtcgt cgagatgagc aggacggcgc agagcaccat gagcgaagcc aggacgatcg
      601 ccgaccacga cagggcgttg atgaaggaga agaagcggtt gagcagctcc ttctggtcct
      661 ggacccggcc gaccccggga gccccctgga acgagctcgc gatgacgtcg aacttgctcg
      721 ggtccgacag cgacacgcgg aagctgtcgg cgaaggcgcg ttccgtcgcc tgctcgccga
      781 tcggcgagtt cttgaactgc gccttgaacc gggcgaaggc ctcggcctgg ctctcgtaga
      841 acacgtcctt gacgggcggg cgcatctgct cgagctgggc gcggatctgg tcccgctggc
      901 cctcggtcgt cgccttgccc gcgcagttcg ggtagagcga cttgtccacg cacaggaaga
      961 tcgagacctg gacccgctcg taccagtagt ccttcatcgt gtcggcctgg cgctgggcga
     1021 gcatgccgag cccgagcagg tacatcgaga tcatcgtgac gaggacgacc gacaccgcca
     1081 tcgacaggtt gcggcgcatc ccgttggccg tctcgctgag caggaacccc ggcttcatcg
     1141 gctcactcac ctgcctcggt cgacgcgccg ggatgctcgg gctgcatctg ttggcccggt
     1201 cccgcgggcc cgggctcccc tgcccccgca gccgggggct ccactgagcc cgcagacgtg
     1261 gcgagctgac cgccataccg gccgtcgctc tggtcgcgca cgagcactcc ctcgtcgagc
     1321 tcgacgaccc ggcggcggcg cgtgttgacc atctcgtggt cgtgggtggc catgaggacc
     1381 gtcgtcccgc cgcggttgat ccgctgcagg agggtgacga tgtccaggct ggtcgtcggg
     1441 tcgaggttgc ccgtgggctc gtcgcagagc aggatcgcag gcttgttgac gaccgcgcgc
     1501 gcgatggcga cccgctgctg ctcgccgccg gacagctcgt gcgggaagcg cttctccatc
     1561 ccctcgaggc ccaccatggc cagggcctcg ggcaccgtct ggcggatgac cgagcgcggc
     1621 ttgccgagca cctgcagggc gaaggcgaca ttggccgaca ccgtcttgtt gggcagcagc
     1681 cggaagtcct ggaagaccgt gccgatctcg cgacgcagcc gcggcacctt gcggtaggcc
     1741 aggcggccga gatcacgacc ggcgacgtgg atcgtgccgg acgtggcgtt ctcctccttg
     1801 aggatgagcc gcagcatcgt cgacttgccc gatccggact tgccgacgac gaagacgaac
     1861 tcgccccgct cgatctccac ggagaccctg tcgagggcgg gccgggtggt cctggggtag
     1921 accttgctga cctggtcgaa cgagat
//
LOCUS       DATAIX010000085         1742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_240358, whole genome shotgun sequence.
ACCESSION   DATAIX010000085 DATAIX010000000
VERSION     DATAIX010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240358"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..789)
                     /locus_tag="VHM65_01335"
     CDS             complement(64..789)
                     /locus_tag="VHM65_01335"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEX2704373.1"
                     /translation="MTVTTGPWTRSTLARWAGGLYLAYILASVLASRLGHIGLSDAQT
                     LRARISGDEAMFRLGLVAALGSALLFVLTAWALYALLRPVDEPLALLVLVLNAVGVAV
                     QCASYLPLLAVLAQGDSAVDVHALTGPQLDGLALLSVTTYRIGFLTAQLFFSAWLFPL
                     GWLVLRSGFLPRFLGWLLLLDGVAVLVWFLQVLLAPGYPAISYPAWAVGFIAELGLGL
                     WLLVKGVRPQVPAPDGREAAGRE"
     gene            complement(786..1193)
                     /locus_tag="VHM65_01340"
     CDS             complement(786..1193)
                     /locus_tag="VHM65_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704374.1"
                     /translation="MANLVISMTPVAAGYRSALSIPYLPMLLEAAAGGLVVAGAVAFL
                     LVRYPQRVPGAGPLRKALLLGLGALVLLTVLVEVPSKLRSDLADPGHWLLVATVFNAI
                     RILALGVTIGLVTRARRTRPDPHRPVTRRQAQS"
     gene            complement(1389..1643)
                     /locus_tag="VHM65_01345"
     CDS             complement(1389..1643)
                     /locus_tag="VHM65_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704375.1"
                     /translation="MDHPGPGEQRDTSSQGGVVLPNTRNGGRRYVARTFPRSLLVRQQ
                     AQPFVSRPNRVDVTTLKELVEAERVAPAGAAQGAAGRGPV"
BASE COUNT          280 a          607 c          640 g          215 t
ORIGIN      
        1 tacacgtcct ctgccgcctc ttgggacgat ggacgtgcct gccgacgggt acgcgggcgg
       61 cctctactcg cggccggccg cctctcgccc gtcgggggcc ggcacctgcg gcctcacgcc
      121 cttcacgagg agccacagcc cgaggccgag ctcggcgatg aagccgacgg cccacgctgg
      181 atagctgatc gcggggtacc ccggggcgag cagcacctgg aggaaccaga cgagcactgc
      241 gaccccgtcg aggaggagaa gccatccgag gaagcggggc aggaacccgg agcgcagcac
      301 gagccagccg agggggaaga gccacgcgga gaagaacaac tgggccgtga ggaacccgat
      361 ccggtatgtg gtgaccgaca gcagggccag cccgtcgagc tgcgggccgg tcagagcgtg
      421 cacgtccacg gcgctgtcgc cctgcgcgag gacggccagg aggggcagat agctcgcgca
      481 ctgcacggcc accccgacgg cgttgaggac gaggacgagc agcgccagcg gctcgtcgac
      541 cgggcggagc agcgcgtaga gcgcccaggc cgtgaggacg aagagcaggg ccgagccgag
      601 cgccgcgacg agccccaacc ggaacatcgc ctcgtccccg gagatcctcg cgcgcagggt
      661 ctgcgcgtcg ctcagcccga tgtggccgag ccggctcgcg agcacgctgg cgaggatgta
      721 cgcgaggtac aggccaccgg cccaccgggc gagcgtgctg cgggtccacg gccctgtcgt
      781 gacggtcatg actgtgcctg ccgcctcgtc accggacggt gagggtctgg tcgggtcctt
      841 cgtgcgcggg tgaccagccc gatggtgacg ccaagggcca ggatgcggat cgcgttgaac
      901 accgttgcca cgagcagcca gtgaccgggg tcggccaggt ccgaacgcag tttcgagggc
      961 acctcgacga gcacggtgag caggacgagg gcacccaggc cgagcagcag ggccttgcgc
     1021 agcgggccgg cgccggggac ccgctgcggg taccgcacga gcaagaaggc caccgcgccc
     1081 gcgacgacca gcccgccggc cgccgcctcc aggagcatcg gcaggtaggg gatcgacagc
     1141 gcggaccggt atccggcggc gaccggggtc atcgagatga cgaggttggc caaccagaac
     1201 gcggccgctc ccgcgagggc gggaacccac cacccagtcc cggccggcct ggtcggcctc
     1261 ggactccggg tacccgacgg ggggacctcg acgatgtccg acaccaactc cgaggggccg
     1321 gacccactgc ccggctcgcg tccagcatcc cgcggggaga tcctgatgac ggccggctgg
     1381 ctgcaaggtc acaccggccc gcggcccgcc gccccctggg cggctcccgc gggagcgacg
     1441 cgctccgcct cgacgagctc cttgagggtc gtcacgtcga cccggttcgg tctcgagacg
     1501 aagggctgtg cctgctggcg cacgagcagt gagcgcggga acgtcctggc gacgtagcgc
     1561 ctgccaccgt tccgtgtgtt gggcaggacg acgcctccct ggctcgaggt gtctcgttgt
     1621 tcaccggggc caggatggtc catatcctga ggcttgtgtc cctcgcacag ccgcttctgg
     1681 cggagccctt cagtgctgag ctgctcggtt actgctaccg cttcttcggg tgctccccga
     1741 tg
//
LOCUS       DATAIX010000086         1691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_34477, whole genome shotgun sequence.
ACCESSION   DATAIX010000086 DATAIX010000000
VERSION     DATAIX010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34477"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /gene="lexA"
                     /locus_tag="VHM65_01350"
     CDS             <1..696
                     /gene="lexA"
                     /locus_tag="VHM65_01350"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX2704376.1"
                     /translation="QMPDRERGDGLTVRQRRVLEVIRNSVDRRGYPPSLREIGEAVGL
                     TSPSSVAHQLAALERKGYLRKDPNRPRALEVVSPDAPSGERGYRQHAQPAAEHDETGS
                     GDARPEASYVPVVGRIAAGGPILAEEAIEDVFPLPKQIVGEGQLFLLRVIGDSMVDAA
                     ICDGDWVVVRQQPTANNGDIVAALLDSEATVKTFKRTSGKVWLLPHNAAFAPIDGDRA
                     TILGKVTAVLRRI"
     gene            complement(743..1573)
                     /locus_tag="VHM65_01355"
     CDS             complement(743..1573)
                     /locus_tag="VHM65_01355"
                     /inference="COORDINATES: protein motif:HMM:NF003738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HEX2704377.1"
                     /translation="MPEDLGPWVELVRGGGVVVLSGAGLSTESGIPDYRGPDGLRRVT
                     PMQYGEFVSSGAARQRYWARSYVGWARLASAGPNDGHRAVAALERLGAVDLVITQNVD
                     GLHQRAGTREVVELHGSLAEVVCLRCGDRTSRVDLDRRMRDANPSYEVRTNEIRPDGD
                     ISLDDLDVAGFVAPRCLVCGEDMLKPDVVFFGESVPRARVDRCFDAVDRAQALLVLGS
                     SLAVMSGYRFVRRAAARGTPVAILTLGPTRGDEQATLRLQAPLGAQLTRLVAVLGRPG
                     "
BASE COUNT          262 a          623 c          591 g          215 t
ORIGIN      
        1 cagatgcccg atcgcgagcg aggcgacggt ctgaccgtgc gccagcgcag ggtgctcgag
       61 gtcatccgca actcggtcga ccggcgcggc tacccgccga gcctgcgcga gatcggggag
      121 gccgtggggc tgaccagccc cagcagcgtc gcccaccagc tggcggctct ggagcgcaag
      181 ggctacctgc gcaaggaccc caaccgcccg cgcgccctcg aggtggtctc ccccgatgcg
      241 ccgagcggcg agcggggcta ccggcagcac gcccagcccg cggccgagca cgacgagacg
      301 ggcagcggcg acgcgcggcc cgaggcgtca tacgtgccgg tcgtgggccg gatcgccgcg
      361 ggcggtccca tcctggccga ggaggccatc gaggacgtct tcccgctgcc gaagcagatc
      421 gtcggtgaag ggcagctctt cctgctccgg gtgatcggtg actcgatggt cgatgcggcc
      481 atctgcgacg gcgactgggt cgtcgtgcgc cagcagccga cagccaacaa cggggacatt
      541 gtcgccgcct tgctcgacag cgaggccacc gtcaagacct tcaagcgcac gagcggcaag
      601 gtctggctcc tgccgcacaa cgcggccttc gcgccgatcg acggtgaccg ggcgacgatc
      661 ctcggcaagg tcaccgcggt cctgcgccgc atctgagcca ccgtatggcg gtcgcgcacc
      721 tcggcgctga gccggcgagg tcctagcctg gccggccgag gacggcaacg agccgggtga
      781 gctgcgcgcc cagcggggcc tgcaggcgca aggtggcttg ctcgtctccg cgagtcgggc
      841 cgagggtgag gatcgcgacc ggtgtccccc gcgccgcagc cctgcggacg aaccggtatc
      901 ccgacatcac cgccagcgac gagccgagca cgagcagggc ctgcgctcgg tccactgcgt
      961 cgaagcaccg gtccaccctg gcgcggggca ccgactcgcc gaagaagacg acgtccggct
     1021 tgagcatgtc ctccccgcag accaggcacc ggggcgccac gaagcctgcc acgtcgaggt
     1081 cgtcgagcga gatgtccccg tccgggcgga tctcgttcgt cctcacctcg tatgacgggt
     1141 tggcgtcgcg cattctcctg tcgaggtcca ctcggctggt ccggtcgccg cagcgaaggc
     1201 acacgacctc ggcgaggctg ccgtgcagct cgacgacctc gcgcgttccg gcacgctgat
     1261 gcagaccgtc gacgttctgg gtgatgacca gatcgacggc gccgagccgc tccaaggccg
     1321 ccaccgcacg gtgcccgtcg ttggggccgg ccgacgccaa gcgcgcccac ccgacatacg
     1381 acctggccca gtagcgctgg cgggcggcac ccgaggagac gaactcgcca tactgcatcg
     1441 gggtgacccg gcgcagtccg tctggtcccc gatagtccgg aatgccggac tcggtcgaca
     1501 gcccggcgcc gctgaggacg accacgcccc cgccgcgcac gagctcgacc cacgggccca
     1561 ggtcctccgg cacgcggtca gtatctccca ccgggtaggc gacccggcgg gcggggccgg
     1621 cggtcccagg cgcgcctgcg gtatcagcac gccatgccaa ccccgacaga ttggataacg
     1681 acccgttatg c
//
LOCUS       DATAIX010000087         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_4350, whole genome shotgun sequence.
ACCESSION   DATAIX010000087 DATAIX010000000
VERSION     DATAIX010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4350"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..1334)
                     /locus_tag="VHM65_01360"
     CDS             complement(246..1334)
                     /locus_tag="VHM65_01360"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2704378.1"
                     /translation="MPAPVLHLTGHVLIGPDDVRDEVWVIGGRIAYDAPGTGADVETV
                     TGWVLPGYVDAHCHVGLDQRGAVDAATAEAQAVADRDAGALLIRDAGCPSDTRWVDER
                     DDLPRIIRAGRHIARTRRYIRGYGHEIEPEDLVTYVRREAHRGDGWVKLVGDWIEREA
                     GDLAPCWPREAVVAAVAAAHEEGARVTAHCFGEESLRDFAAAGTDCIEHATGLQPDTT
                     AAFAEQQIAIVPTLVNIANFPSFAEAAREKFPAYAAHMVDLFDRRYATIAAAHDAGVP
                     VYLGTDAGGALPHGLVAMEARELVHAGFSAAEATNAATWAARRWLGRPGLDEGASADL
                     VVLDEDPRADVAVLAAPRHIVMRGRVFR"
BASE COUNT          199 a          546 c          564 g          200 t
ORIGIN      
        1 atgttggtga tgctgacctt gcccacgagg tcgtaggcac cctcgggctc gcgggcatgg
       61 acgagcaggg tgcggtgggt cggtgactga gccccgacca ggcccgccag cgcgtggggt
      121 cggccgggcc gaactgcgcg aggcgtgccc gcgactgctt caccgcgatg acgtgtgacg
      181 cgatgtcggc tccggtcgca ggggccaccc agacccgctc ccccgaccga gcgagcggca
      241 cgacgtcagc ggaagacacg cccgcgcatc acgatgtggc ggggagcggc cagcacggcc
      301 acgtcggctc gcgggtcctc gtcgaggacg acgaggtcgg cgctggcacc ctcgtccagg
      361 ccgggccggc cgagccagcg ccgggcggcc caggtcgccg cgttcgtggc ctccgcagcg
      421 ctgaacccgg cgtggacgag ctcgcgggcc tccatcgcaa ccagcccatg gggcagggct
      481 ccaccggcgt cggtgccgag gtagacgggg actccggcgt cgtgcgcagc cgcgatggtc
      541 gcgtagcggc ggtcgaacaa gtcgaccatg tgcgcggcat acgccgggaa cttctcgcgg
      601 gctgcctccg cgaacgacgg gaagttggcg atgttgacca gggtggggac gatcgcgatc
      661 tgctgctcgg cgaacgcggc cgtcgtgtcg ggctgcagcc ccgtggcgtg ctcgatgcag
      721 tcggtcccgg cggccgcgaa gtcgcgcagg gactcctctc cgaagcagtg ggccgtgact
      781 cgggcgccct cctcgtgggc ggcggcgacg gcggccacga cggcctcgcg tggccagcac
      841 ggtgccaggt cgcccgcctc gcgctcgatc cagtccccga cgagcttgac ccagccgtcg
      901 ccgcggtgag cctcccggcg gacataggtg acgaggtcct cgggctcgat ctcgtggccg
      961 tagccgcgga tgtagcgcct ggtccgggcg atgtgccggc cagcacggat gatccggggc
     1021 aggtcgtcgc gctcgtcgac ccaccgggtg tccgaggggc acccggcgtc gcggatcagc
     1081 agggccccgg cgtcacggtc ggccacggcc tgggcctcgg ccgtcgcggc gtcgacggca
     1141 ccgcgctggt cgaggccgac gtggcagtgc gcgtcgacat acccgggcag gacccagccg
     1201 gtgacggtct cgacgtcggc gcccgtgccg ggggcgtcat acgcgatccg gcccccgatg
     1261 acccagacct cgtcacggac atcgtccggg ccgatcagga catgacccgt gaggtggagc
     1321 accggcgcag gcatggatgc actctagcca ggcaccgagc ggcgcgcctc cccgccctgg
     1381 acccggtgag gtcgccgcgt tcgccctcgc cgaccggccg tggggcaccg gctccggacc
     1441 ctgcacgcag cccgcgacct tcgtccaccg cctgcccgcg gaccgaggct cgagtaccct
     1501 tctctagta
//
LOCUS       DATAIX010000088         2882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_34556, whole genome shotgun sequence.
ACCESSION   DATAIX010000088 DATAIX010000000
VERSION     DATAIX010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34556"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VHM65_01365"
     CDS             complement(<1..313)
                     /locus_tag="VHM65_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704379.1"
                     /translation="MSSCSSLRIGLLGSVLPFDSGAVPWGAVPGCAGLSLSLSLWLVA
                     DEAGVVVSALAVSAAPPTAMPATAAMAAAVVMMMLLLDFFMVVPPGSSGSVAMWLPSV
                     PA"
     gene            288..569
                     /locus_tag="VHM65_01370"
     CDS             288..569
                     /locus_tag="VHM65_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704380.1"
                     /translation="MRSDEQLLTGDIAAKVTAAAKAKEPDATIQRVETDSEGVYEAHM
                     VRADGTAIIVQVDKTFTVTNVQESPGGGGAGAGGTAPQGAPGESGTATT"
     gene            complement(650..1174)
                     /locus_tag="VHM65_01375"
     CDS             complement(650..1174)
                     /locus_tag="VHM65_01375"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEX2704381.1"
                     /translation="MFGSIGGLPIHALVIHVVVILAPLSALLALAYALAPRWRRTLRW
                     PTALGALVTGVSGVVAGESGKWLDERVIASIVPTDPTVAAHLKALGEHVAAGERLEKV
                     CVAFMIVMLATVFWAAPAPAAERGDAPASGARTVDTVARVVVVLLALLLIALTIQTGH
                     LGATAVWGTLLTTS"
     gene            1388..1636
                     /locus_tag="VHM65_01380"
     CDS             1388..1636
                     /locus_tag="VHM65_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX2704382.1"
                     /translation="MATMNISAARENLAAAVELSRTEAVFLERYGRRAAVLVSPERYD
                     ELMAALEDAQDVAAFDEAMEEEGPNIPWELVKVDLGWA"
     gene            1633..1896
                     /locus_tag="VHM65_01385"
     CDS             1633..1896
                     /locus_tag="VHM65_01385"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX2704383.1"
                     /translation="MSTYRVELRPAAVRALRTLDPQVRGRIQGTIALLAQDPRPPAAR
                     ALQGRPGLRVRVGDYRIIYTVQDDVLLLVVVVTLGHRREVYER"
     gene            1986..>2882
                     /locus_tag="VHM65_01390"
     CDS             1986..>2882
                     /locus_tag="VHM65_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704384.1"
                     /translation="MSRLESQGPPPYEQVYPRLTDIDLRADATRLDVPVHLVQGGHET
                     RGRAELVTEWFDRLAAPRKQVVVRSCSPIPPGADDRFGHANERRAAMKPSRVVLLVLG
                     SLVALMGFGLLAGGATLGWALASQRDSAGFFSTPVERFATDSAAVTFDDIDLGEPGVD
                     EWWATRDLATVRIGVDSATSADLFVGIGPEADVEAYLAGVPHDKVTNVRYRPFAADYR
                     HIDAAGQAVPAAPGEQTSWVAKASGSGAQTLTWDIAPGRWAVVVMNADGSPGVGADVT
                     LAGRTDFLVPLAGGLGLFGVLLA"
BASE COUNT          470 a         1009 c         1035 g          368 t
ORIGIN      
        1 gggccggcac cgacggtagc cacatggcaa cactgccaga gctaccagga ggcaccacca
       61 tgaagaagtc cagcagcagc atcatcatca ccaccgccgc agccatcgcg gccgtggccg
      121 gcatcgccgt cggcggcgcc gcgctcacgg cgagcgcgga caccaccacc ccggcctcgt
      181 ccgcgacgag ccagagcgag agcgagagcg agagcccagc gcagccgggc acggcgcccc
      241 agggcacggc gccagagtcg aacggcaaca cggacccgag caggccgatg cgcagcgacg
      301 agcagctgct caccggggac atcgccgcca aggtcaccgc ggccgccaag gccaaggagc
      361 cggacgcgac gatccagcgg gtcgagaccg actccgaggg cgtgtacgag gcgcacatgg
      421 tccgagccga cgggacggcc atcatcgtgc aggtcgacaa gaccttcacg gtgacgaacg
      481 tccaggagag cccgggcggc ggaggcgccg gggccggcgg cacagccccg cagggtgccc
      541 caggggagtc ggggacggcc acgacctgaa gccggacgac gagctcacgg gttgggtgcc
      601 cggcatacgg caaccgtgcc gtatgccggg gacccacccg tgaggtgcgt caggaggtcg
      661 tgagcagggt gccccacacc gcggtggcac cgaggtgccc ggtctggatg gtcagggcga
      721 tgaggagcag cgcgagcagg acgacgacga cgcgcgcgac cgtgtcgacc gtccgggcgc
      781 cgctcgcggg cgcatcgcca cgctctgccg ccggcgcggg cgcggcccag aacaccgtcg
      841 ccagcatgac gatcatgaag gcaacgcata ccttctccag ccgctccccg gcggcgacgt
      901 gctcgcccag ggccttgagg tgggcggcca cggtggggtc ggtcgggacg atcgaggcga
      961 tgacccgctc gtcgagccac ttgccgctct ccccggccac gaccccggag acgccggtga
     1021 ccagggcacc gagcgcggtc ggccagcgca gcgtccggcg ccaccggggc gccagcgcgt
     1081 aggccagagc cagcagggcc gacaacggag ccaggatgac gacgacgtgg atgacgagcg
     1141 cgtggatcgg caggcccccg atggaaccga acatggtcaa cctcttcccg gcctgcgccg
     1201 tctcgtcccg ccaagttgaa cccgcaacca ccctagacag gggatcgggt ctgcgcgagc
     1261 ccgcgcgccc aggccatcgg gaccggacgc ggtgccacag ggcaagggcg ggacgctggg
     1321 cggcagatgg gcatcgcagg ctttgacagt cttgtacgtc ttttacgtca catcgtacga
     1381 tagcgggatg gcgacgatga acatcagtgc ggctcgcgag aacctcgccg ctgccgtgga
     1441 gctgtctcgc acggaggcgg tgttcctcga gcgctatggc cgtcgcgcgg cagtactcgt
     1501 cagtcccgag cgctacgacg agctgatggc cgcactggag gacgcgcagg acgtggctgc
     1561 cttcgacgag gccatggagg aagagggtcc gaacatcccc tgggagctgg tcaaggtcga
     1621 tctcggctgg gcgtgagcac gtaccgcgtc gagctcaggc cggccgcggt ccgggcactt
     1681 cgcacgctcg acccccaggt ccgcgggcgc atccagggca cgatcgccct gctcgcgcag
     1741 gacccacgac cgccagcggc ccgggccttg cagggccgcc cgggtttgcg ggttcgggtc
     1801 ggtgactacc gcatcatcta caccgtgcaa gacgatgtcc tcctcctcgt ggtggtcgtc
     1861 acgctcgggc acagacgaga ggtctacgaa cgctgatcgc cgtcccaatc gccgatccgc
     1921 ttacgggccg ccagccggcc tggccgtgcg gatgctgaga cgttcggtcg gggaacgccg
     1981 ccctggtgag caggctcgag agccagggcc caccgccata cgagcaggtc tacccccgcc
     2041 tgacggacat cgacctgcgg gccgacgcca cccggctgga cgtgccggtc catctggtcc
     2101 agggtggaca cgagaccaga ggccgggccg agctcgtcac cgagtggttc gaccggctcg
     2161 ccgcacctcg caagcaggtc gtcgtcagat cgtgctcgcc gataccaccc ggagcggatg
     2221 accgcttcgg tcacgccaac gaaaggaggg ccgccatgaa gccgtcgcgc gtcgtgctgc
     2281 tggtcctggg ctcacttgtc gcactcatgg gtttcgggct cctcgccgga ggcgccacgc
     2341 tgggctgggc cctggcgagc cagcgtgaca gtgccgggtt cttctcgacc cccgtggagc
     2401 ggtttgcgac ggacagcgcc gcggtgacct tcgacgacat cgacctcggc gaaccggggg
     2461 tcgacgaatg gtgggccacc cgtgacctcg ccaccgtccg gatcggcgtg gacagcgcga
     2521 cctcggcgga cctcttcgtc ggtatcggtc cggaggccga cgtcgaggcg tacctggccg
     2581 gggtgccgca cgacaaggtc accaacgtcc gctaccgtcc gtttgccgcg gactatcgcc
     2641 acatcgacgc cgcgggacag gccgtgcctg ccgcaccggg cgagcagacc tcctgggtcg
     2701 ccaaggcatc cgggagtggc gcgcagacgt tgacgtggga catcgcgccc ggccggtggg
     2761 cggtcgtcgt gatgaacgcc gacggctcgc cgggggtcgg cgcggacgtc accctggctg
     2821 gccggaccga cttcctcgtc ccgctcgccg ggggcctggg gctgttcggg gttctcctgg
     2881 cc
//
LOCUS       DATAIX010000089         5903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_240458, whole genome shotgun sequence.
ACCESSION   DATAIX010000089 DATAIX010000000
VERSION     DATAIX010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240458"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1364)
                     /locus_tag="VHM65_01395"
     CDS             complement(18..1364)
                     /locus_tag="VHM65_01395"
                     /inference="COORDINATES: protein motif:HMM:NF005883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone dehydrogenase"
                     /protein_id="HEX2704385.1"
                     /translation="MSSRGPSVVILGGGPGGYEAALVAAQLGARVTVVDCDGLGGAAV
                     LTDCVPSKTLISIAGDHRYARLGEINRRILGLAQAQSEDIHRRLESDGVRIVCGTGRL
                     VSPTQVRAVLDDGSSEDLVAEVVLVATGAAPRIMATAQPDGERILTWQQIYGLADLPE
                     RLVVVGSGVTGAKLAAAYLALGSAVTLVSSRDRVLPGEDADAAAVIEDVFRRNGMEVL
                     DRSRMSSVERVGEEVRVHLEGGRVVTGTHALLALGSIPRTAGIGLAEAGVELAPSGHV
                     VVDGVSRTSVRSVYAAGDCNGRLPLASVAAMQGRIAMWHALGDAVAPLRLDIVSANIF
                     TTPEIATVGVSQKDVDSGRVEAQSVTLPLDTNARAKMDDFEDGFVKLFARPGTGAVLG
                     GVIVAPRASELIYPVAMAVTYGLTVDQVAGTFTVYPSMTGSIAEAARRLHPRPLAR"
     gene            1530..2672
                     /locus_tag="VHM65_01400"
     CDS             1530..2672
                     /locus_tag="VHM65_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2704386.1"
                     /translation="MSSTPIRRIAGVAVLAFAVGGVVACSPPADSPSTGASSSSGGVS
                     AKTATSAKAFGGMEALVAAAKQEAALNVIALPPNWANYGEIIKGFQAKYGIKITSDQP
                     EGTSADEISSAQRLKGQDKAPDVFDLGTTVALANVSMFAPYQVEKWSEIPDNRKEAAG
                     VWVNDYAGYMTVGYDSAKVPAPTAIDDLLKAEYKGKVALNGDPTKAGAAFAAVQMVSV
                     AKGGTVADVAKGVDFFKALKAAGNFLPVDPTDATIESGQTPVVFDWAYNNAPRVAKVP
                     TWKTWIPPNAVVAGFYVQAINNDAPHPAAARLWQEYLYSDEGQNLWLKGAAFPVRYDA
                     MKAAGTLDAASVAKLPEVSGSAIIPTDAESTTAKDYLAKNWANAIG"
     gene            2701..3549
                     /locus_tag="VHM65_01405"
     CDS             2701..3549
                     /locus_tag="VHM65_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704387.1"
                     /translation="MQPSGALLGVVPFFAYTTVFLIIPTLVVVVGAFLAKSGGFTLTN
                     VQGLGEPAVTKALTQSIVLSVVTALAGALIGAVLAYAVSTAKPDGTLKRFVTSLCGVL
                     AQFGGVALAFAFLASFGATGFLTAGLSKLGIDSANGTWIYEWNKGLMLVYLYFQIPLM
                     LIVFLPAVEGLRPQWREATETLGGTTWTYWRKVGAPILLPSFVGATLLLFTNAFSAYA
                     TAAALVSQGSPLLPLAIGGTFSGEVITGQENVGKAMALAMVVVVAIVMVFYAWLDKRS
                     SRWLVR"
     gene            3546..4397
                     /locus_tag="VHM65_01410"
     CDS             3546..4397
                     /locus_tag="VHM65_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2704388.1"
                     /translation="MRGQRARLRQRQSWLRRAVIGVVLVFFALPLIGMLEFTTQGPGG
                     VGRSTQTWANLLDFPKIETDYPALKEGFLASLGLALVTVVIMLVLLVPTMTWVRLRLP
                     GLSRLVEFICLLPLTVPAIVLVVGLTPVYAWVTYLLGSSTLWLSLAYVILVLPYAYRS
                     LDAGLRAIDVKTLSEAARSLGASWLTVMRKIVLPNLRTAVLSASFLSVALVLGEFTIA
                     NLFARKNLQVAMYELGKSDAKISVAVGLAALAFAFAVLFGMSFIGNARSGPSPVARLR
                     RHRKESA"
     gene            4394..5455
                     /locus_tag="VHM65_01415"
     CDS             4394..5455
                     /locus_tag="VHM65_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2704389.1"
                     /translation="MSAPGTTGGVAVALTHLTREYAGGIRALDDFTLHIQPGEFVALL
                     GPSGCGKTTALRCLAGLEDPNSGTVEVGGTDLTSVPTNKRDMGMVFQAYSLFPHMTAR
                     QNVEFGLQLRKRSAEARRARTEEMLALVGLEAQADRYAHQMSGGQQQRVALARALAIE
                     PQVLLLDEPLSALDAKVRTQLRDEIRRIQLEVGTTTLFVTHDQEEALAVADRVGVMRA
                     GTLEQLAPPQELYARPATAFVADFVGLTNRLKGVSAGSHAQVLGVSIPLLPGSATSGP
                     VEVLVRPEAIGVAPDDGGQASVVSASFLGAHGRVIATTDFGERVVVQVSSSAVTSYPP
                     GSRVRLTPSDNPALAVATG"
     gene            complement(5469..5663)
                     /locus_tag="VHM65_01420"
     CDS             complement(5469..5663)
                     /locus_tag="VHM65_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704390.1"
                     /translation="MVEARAGLLPVAASVDDASEAELSRSTGPSPASTPTWDEFRAAM
                     AAERRFPLILDIEQIYAMAS"
BASE COUNT          880 a         2168 c         1975 g          880 t
ORIGIN      
        1 ccccggaagg ggtctactca ccgggcgagg gggcgcgggt gcaggcgccg ggcggcctcg
       61 gcgatcgagc cggtcatcga cgggtagacg gtgaaggtcc cggcgacctg gtcgacggtc
      121 agcccgtagg tcacggccat cgccacgggg tagatcagct cggacgcgcg gggggcgacg
      181 atgacaccgc cgagcacggc ccccgtgccg gggcgggcga agagcttgac gaagccgtcc
      241 tcgaagtcgt ccatcttggc gcgggcattg gtgtcgagcg ggagcgtgac cgactgggcc
      301 tcgacccgcc ctgagtccac gtccttctgc gacacaccga cggtagcgat ctcgggcgtc
      361 gtgaagatgt tggccgacac gatgtcgagc cgcagcggcg cgaccgcgtc accgagcgca
      421 tgccacatcg cgatccggcc ttgcatggcc gccaccgagg ccaggggcag ccgcccgttg
      481 cagtctcctg cggcatacac ggaccggacc gatgtccgcg agacgccgtc gacgacgacg
      541 tgcccggacg gtgcgagctc gacgccggct tcggccagcc cgatccccgc cgtgcgcggg
      601 atcgacccga gcgccagcag tgcatgagtg ccggtcacga cccggccgcc ctcgaggtgg
      661 acccggacct cctcgccgac ccgctcgacc gaggacatcc gcgaccggtc gagcacctcc
      721 atcccgttgc gccggaacac gtcctcgatc acggccgcgg cgtcggcgtc ctcgccgggt
      781 agcacccggt cgcgtgagga cacgagcgtc accgcactgc ccagggcgag gtatgccgcc
      841 gccagcttgg ctcccgtgac accggacccc acgacgacga gccgttcggg caggtcggcg
      901 agcccgtaga tctgctgcca ggtgaggatg cgctcgccgt cgggctgggc ggtcgccatg
      961 atgcgggggg cggcaccggt ggcgacaagg acgacctcgg caacgaggtc ctcgctcgac
     1021 ccgtcgtcca gcaccgcccg aacctgggtc ggcgagacca gccggccggt cccgcacacg
     1081 atgcgcacgc catcggactc gagccggcgg tggatgtcct cactctgggc ctgggccagc
     1141 ccgaggatgc ggcggttgat ctcaccgaga cgggcatagc ggtggtcgcc cgcgatcgag
     1201 atgagggtct tgctggggac acagtcggtg agcacggccg cgccgccgag cccgtcacag
     1261 tcgaccacgg tgacccgggc accgagctgg gccgcgacga gggcggcttc gtacccaccc
     1321 ggtcctccac cgaggatgac gaccgacggg ccacgcgaac tcacgcgaac catccaacca
     1381 cgcgccgggt aacacctggg gaacagagcg gggtcggcta gagacggcga tttccccgtg
     1441 tgctaagcat gaaccaccac gaggcgacgt ggtggtccgg ggtaccccgg gagccgtggt
     1501 cgcgtcatac aaggaccgag gaggttcccg tgagctctac ccccatccgc cggatcgcag
     1561 gtgtcgccgt cctggcgttc gccgtcggcg gtgtcgttgc ctgctccccg cccgcggaca
     1621 gcccgagcac gggtgcgtcg tccagctccg gcggtgtgtc ggcaaagacg gcgacctccg
     1681 ccaaagcctt cggcggcatg gaggccctcg tggccgccgc caagcaggaa gccgcgctca
     1741 acgtcatcgc cctgccgccg aactgggcca actacggcga gatcatcaag ggcttccagg
     1801 ccaagtacgg catcaagatc acgagcgacc agcccgaggg caccagcgcc gacgagatct
     1861 cgtccgcaca gcggctcaag ggccaggaca aggcgcccga cgtcttcgac ctcggcacca
     1921 cggtcgccct ggccaacgtg agcatgttcg cgccatacca ggtcgagaag tggtcggaga
     1981 tcccggacaa ccgcaaggag gccgccggcg tctgggtcaa cgactacgcg ggctacatga
     2041 cggtcggcta cgactcggcc aaggtcccgg caccgaccgc catcgacgac ctgctcaagg
     2101 ccgagtacaa gggcaaggtc gccctcaacg gtgacccgac caaggccggc gcggcgttcg
     2161 cggcggtcca gatggtctcc gtggccaagg gcggcacggt cgccgacgtg gccaagggcg
     2221 tcgacttctt caaggcgctc aaggccgcgg gcaacttcct gcccgtcgac ccgaccgacg
     2281 cgacgatcga gtccggccag accccggtcg tcttcgactg ggcctacaac aacgcgcccc
     2341 gggtcgcgaa ggtcccgacg tggaagacgt ggatcccgcc gaacgcggtc gtcgccggct
     2401 tctacgtcca ggcgatcaac aacgacgcac cgcaccccgc cgcggctcgc ctgtggcagg
     2461 agtacctcta ctccgacgag ggccagaacc tctggctcaa gggcgcggcc ttcccggtcc
     2521 gctacgacgc gatgaaggcg gcgggcacgc tcgacgccgc gtccgtcgcg aagctgcccg
     2581 aggtgagtgg ttccgccatc atcccgaccg acgccgagag cacgacggcc aaggactacc
     2641 tggccaagaa ctgggccaac gccattggct gaccgccaac gcaggggggc gctcggcggc
     2701 atgcagccgt ctggcgccct cctcggcgtc gtccccttct tcgcctacac gacggtcttc
     2761 ctcatcatcc cgacactcgt cgtggtggtc ggcgccttcc tcgccaagtc gggcggcttc
     2821 acgctgacca acgtccaggg cctgggcgag ccggccgtca cgaaggcact cacccagtcg
     2881 atcgtcctgt cggtggtcac cgccttggcc ggcgccctga tcggggcggt cctcgcgtat
     2941 gccgtgagca cggcaaagcc ggacggaacc ctcaagcgct tcgtcacctc gctgtgcggc
     3001 gtgctcgccc agttcggtgg cgtggcactg gcattcgcgt tcctggccag tttcggggcg
     3061 accgggttcc tcaccgccgg cctgagcaag ctcggcatcg actcggcgaa cgggacctgg
     3121 atctacgagt ggaacaaggg cctcatgctc gtctacctct acttccagat cccgctcatg
     3181 ctcatcgtct tcctgcccgc cgtcgagggc ctgcgcccgc agtggcgcga ggccaccgag
     3241 accctcggcg ggacgacgtg gacctactgg cgcaaggtgg gggcgccgat cctgctcccg
     3301 tccttcgtcg gagcgacgct gctgctcttc accaacgcct tcagcgccta cgcgaccgcc
     3361 gcagccctgg tctcgcaagg gtccccgttg ttgcccctgg ccatcggcgg cacgttctcc
     3421 ggcgaggtca tcaccggtca ggagaacgtc ggcaaggcga tggcgctcgc gatggtcgtc
     3481 gtcgtcgcga tcgtcatggt gttctacgcc tggctcgaca agcgctcgag cagatggctg
     3541 gtccgatgag gggccagcgg gcgcgtctgc gtcagcgcca gagctggctg cgccgggccg
     3601 tcattggcgt ggtcctcgtc ttcttcgccc taccgctcat cggcatgctc gagttcacga
     3661 cccagggtcc cggcggcgtc ggccgcagca cccagacgtg ggcgaacctg ctcgacttcc
     3721 ccaagatcga gacggactac cccgcgctca aggagggctt cctcgcctcg ctgggcctcg
     3781 cgctggtcac cgtcgtcatc atgctcgtgc tcctcgtgcc gacgatgacc tgggtgcgcc
     3841 tgcgcctgcc ggggctctcg cggctcgtcg agttcatctg cctgctgccg ctaaccgtcc
     3901 cggcgatcgt cctcgtcgtc gggctcaccc cggtctatgc ctgggtgacc tacctgctcg
     3961 gcagctcgac cctgtggctg tccctggcct acgtcatcct cgtgctgccc tacgcctacc
     4021 gctccctcga cgccgggctg cgcgccatcg acgtcaagac cctgtccgag gccgcccgct
     4081 cgctcggggc cagctggctc accgtcatgc gcaagatcgt gctgcccaac ctgcgcacgg
     4141 cggtcctgtc ggcgtcgttc ctctcggtcg ccctggtcct cggtgagttc accatcgcca
     4201 acctgttcgc gcgcaagaac ctgcaggtcg cgatgtacga gctcggcaag agcgacgcca
     4261 agatcagcgt cgccgtcggg ctggcagctc ttgccttcgc gttcgccgtc ctcttcggta
     4321 tgtcgttcat cggcaacgcc cgctcggggc cttcgccggt ggcccggctg cgccgccacc
     4381 gcaaggagtc cgcatgagcg cccccggcac cactggcggc gttgctgtcg ccctgaccca
     4441 cctcacccgc gagtatgccg gcggcatccg ggccctggac gacttcaccc tgcacatcca
     4501 gccgggcgag ttcgtcgccc tgctcggccc gtccgggtgt ggcaagacga ccgcgctgcg
     4561 ctgcctggcc ggtctggagg accccaactc gggcacggtc gaggtcggcg gcacggacct
     4621 cacctcggtt ccgacgaaca agcgcgacat gggcatggtg ttccaggcct acagcctgtt
     4681 cccccacatg accgcccggc agaacgtcga gttcggcctg cagctgcgca agcgctccgc
     4741 cgaggcccgc cgggcccgca ccgaggagat gctcgccctc gtcggtctcg aagcgcaggc
     4801 cgaccgctac gcccaccaga tgtccggcgg gcagcagcag cgcgtcgccc tggcccgcgc
     4861 cctcgcgatc gagccacagg tgctcctgct cgacgagccg ctctccgccc tcgatgccaa
     4921 ggtccgcacc cagctgcgcg acgagatccg ccgcatccag ctcgaggtcg gcacgacgac
     4981 gctcttcgtc acccacgacc aggaggaggc tctcgccgtc gccgaccggg tcggcgtcat
     5041 gcgcgccggc accctcgagc agctggcccc gccacaggag ctctacgccc ggccggcaac
     5101 ggctttcgtc gccgacttcg ttggcctgac caaccggctc aagggggtca gtgccggatc
     5161 ccatgcccag gttctcggcg tgtcgatccc gctcctgcct ggctcggcca cgagcgggcc
     5221 ggtcgaggtg ctggtccggc cggaggcgat cggtgtcgca ccggacgacg gcggacaggc
     5281 cagcgtggtc agcgcgagct tcctcggtgc ccatggccgg gtcatcgcga cgaccgactt
     5341 cggcgagcgg gtcgtcgtcc aggtgagcag ctcggcggtc acgtcatacc cgcccggcag
     5401 cagggtccgg ctgaccccca gcgacaaccc cgccttggcg gtcgccaccg gctgacatca
     5461 gcccagagtc agctcgccat cgcgtagatc tgctcgatgt ccaggatgag cgggaaccgc
     5521 cgctcggcgg ccatcgcggc gcggaactcg tcccaagtcg gggtgctcgc cggcgatggc
     5581 ccggtggatc tggagagctc ggcctcactc gcgtcgtcga ccgacgcggc gaccggcagg
     5641 agcccggcgc gggcctcgac cacggcatac gaccagccgt ccccgctcgt gacgtcgagg
     5701 ctggctcgcg ggtcccgacg cgggaccgcg gcacccgacg ggacatcggt ccccgcacgc
     5761 cacgaccggc ttcgcggatc agcagggctc ggtcgcctcg acgagatcga ggatgaagcg
     5821 ggccatctga gcccgttcgc gggcgttgtg gagcaggtcc tcggtcggct cgtcgtcgag
     5881 gtgctcgacc gggaaggccg aga
//
LOCUS       DATAIX010000090         3323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_181004, whole genome shotgun sequence.
ACCESSION   DATAIX010000090 DATAIX010000000
VERSION     DATAIX010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181004"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..546)
                     /locus_tag="VHM65_01425"
     CDS             complement(64..546)
                     /locus_tag="VHM65_01425"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEX2704391.1"
                     /translation="MGSTLAVAILMLVGVVGIVVPIVPGLAIVWLATALWAWVHPSPA
                     AWVVFGTATFWYVAGLVTQYVVPGRRMKAAGVRTSTLLIALVGAVVGFFVIPVVGGIV
                     GFVLGIYAVELARAGDRSAAWASTKHALRAVGLSMGIELGAALLIVTTWVIGVLALGT
                     "
     gene            complement(550..774)
                     /locus_tag="VHM65_01430"
     CDS             complement(550..774)
                     /locus_tag="VHM65_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704392.1"
                     /translation="MTTALAPSLTAADRCDRCGAQAYIRARLATGGELFFCAHHGRKH
                     LPELQSRCIEIRDETERLGSSAAGEAKAQR"
     gene            1115..3235
                     /locus_tag="VHM65_01435"
     CDS             1115..3235
                     /locus_tag="VHM65_01435"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEX2704393.1"
                     /translation="MSQAAGGTDYTARHLQVLEGLEAVRKRPGMYIGSTDSPGLMHCV
                     GEIIDNAVDEALAGVCDRIEVILASNGSIEVRDNGRGIPVDIEPRTGLSGIEVVFTKL
                     HAGGKFGGGSYTASGGLHGVGASVVNALSARLDVEVDRGGKTYGMSFRRGEPGAFTDA
                     RAAVHSPEATFTPYVVNSELAVVGQAPRGKTGSRVRYWPDRQIFPKDATIEYAALVAR
                     ARQTAYLIPGLTIVLRDERGLAGTPGADGPNEETLCHHGGISEFTEFLAPDPALTDVW
                     RLQGSGTFRETVPVLDDRGHLSPAEVERECGVDIAVRWGTGYDTRVTSFVNIIATPSG
                     GSHLLGFEQALLKVFRRQLELNGRRLRVGADKVDKEDILAGLTAVVTVRLAEPQFEGQ
                     TKEVLGTAAVRAIVARVVEGELTARITSVKRGDKAQAALLLDKVTAEMKSRISARVHK
                     ETQRRKNALETSSLPAKLADCRSTDVERSELFIVEGDSALGTAKLARSSDHQALLPIR
                     GKILNVQKASVSDMLKNAECAAIIQVIGAGSGRSFDLESARYGKVIIMTDADVDGAHI
                     RTLLLTLFFRYMRPLVDAGRVFAAVPPLHRIEVVNAGSARNELIYTYSEPQLRQTLSS
                     LEKRGKKVKQPLQRYKGLGEMDADQLAETTMDPRHRTLRRVTLRDAEIAEQVFDLLMG
                     NDVAPRKDFIIDSAAELDRDRIDA"
BASE COUNT          569 a         1159 c         1135 g          460 t
ORIGIN      
        1 ccccaaaacc gcagccccgc aacgataatc accgcccaac gccatgatca tcggaaactc
       61 gggctaggtg ccgagggcga ggacgccgat gacccaggtc gtcacgatga gcagtgcggc
      121 accaagctcg atccccatgc tgagcccgac cgcgcgaagg gcgtgcttcg tcgaggccca
      181 agccgctgac cggtcgcccg cccgggcgag ctcgaccgcg tagatcccga gcacgaagcc
      241 gacgatcccg ccgacgaccg ggatgacgaa gaagcccacg acagccccga cgagggcgat
      301 gagcagcgtg ctcgtgcgca ccccggcggc cttcatccga cgcccgggga cgacgtactg
      361 cgtcaccagc ccggcgacat accagaaggt ggcagtcccg aacacgaccc acgccgccgg
      421 ggaggggtgc acccaggccc acagggcggt cgcgagccag acgatcgcca agccgggcac
      481 gatgggcacc acgatgccaa cgacccccac gagcatgagg atcgcaacgg caagggtgct
      541 acccaacgat caccgctggg ccttcgcctc gccggccgcg ctgctgccga gacgctcggt
      601 ctcgtcgcgg atctcgatgc accgggactg aagctcagga aggtgcttgc ggccatggtg
      661 cgcacagaag aacagctccc cgccggttgc aaggcgggcg cgaatgtagg cctgggcacc
      721 acagcggtca cagcggtccg cggcggtcag tgagggtgcc agagcagtgg tcacgtcagc
      781 cttctctcat ctcttgtggt gaggagtcgt ctccgacgcc caccgggtgc caccgggtgc
      841 gagcgccctg aggcgaccgg tatccctgtg acgctggttg acggcccgca gttgcctcga
      901 gccgtccacc cgcctcaaca gtgaaccacg gtcacctctt cccagaggcc attgcccgcc
      961 gctagagaca gctcatgtcg tattcgtagc cgggacgtgt ccggatcgtg acgggccctc
     1021 ccgacgggcg cgcctgtgcc gggccgtccg gggaagcggc tagcctgcca ggagcgggca
     1081 ccgggcccgc tgggacacca aggaagggac gcacgtgtca caggccgctg gaggcaccga
     1141 ctacaccgct cggcacctgc aggtgctcga gggcctggag gccgtgcgca agcgtcccgg
     1201 tatgtacatc gggtcaaccg actctcccgg gctcatgcac tgtgtggggg agatcatcga
     1261 caacgccgtc gacgaggccc tcgccggcgt ctgcgaccgg atcgaggtca tcctcgcttc
     1321 caacggctcc atcgaggtcc gcgacaacgg ccgtggcatc ccggtcgaca tcgagccgcg
     1381 caccgggctc tccggcatcg aggtggtctt caccaagctc cacgcaggcg gcaagttcgg
     1441 cggcggctcc tacaccgcct cgggcggcct gcacggagtc ggcgcctcgg tcgtcaacgc
     1501 cctgtccgca cggctggacg tcgaggtcga ccgcggcggc aagacctacg gtatgtcgtt
     1561 ccgccgcggc gagccgggtg ccttcaccga tgccagggcc gcggtccaca gccccgaggc
     1621 gacgttcacg ccatacgtgg tgaacagcga gctggccgtc gtcggccagg caccgcgagg
     1681 caagaccggg tcgcgggtgc gctactggcc cgaccggcag atcttcccca aggacgcgac
     1741 gatcgagtac gccgcgctcg tcgcccgggc gcgtcagacg gcatacctga tccctggcct
     1801 gacgatcgtg ctccgcgacg agcgggggct cgcgggcaca ccgggcgccg acggtccgaa
     1861 tgaggagacg ttgtgccacc acggggggat ctcggagttc accgagttcc tcgcccccga
     1921 cccggccctg accgacgtgt ggcgactgca gggatcgggg acgtttcgcg agaccgtgcc
     1981 cgtcctggac gacagggggc acctgagccc ggccgaggtc gaacgcgagt gcggggtcga
     2041 catcgcggtg cgatggggga ccgggtatga caccagggtc acctcgttcg tcaacatcat
     2101 cgccaccccg tcgggcgggt cacacctgct cggcttcgag caggccttgc tcaaggtctt
     2161 ccgccgccag ctcgagctca atgggcgccg gctcagggtc ggtgcggaca aggtcgacaa
     2221 ggaggacatc ctcgccgggc tgacggccgt cgtgaccgtg cgcctggccg agccgcagtt
     2281 cgagggtcag accaaggagg tcctcgggac cgctgccgtc cgggccatcg tggcgcgggt
     2341 cgtcgagggg gagctgaccg cccggatcac ctcggtcaag cgcggtgaca aggcccaggc
     2401 ggcgctgctg ctcgacaagg tcacggccga gatgaagtcc cggatcagtg cccgggtcca
     2461 caaggagacc cagcggcgca agaacgccct ggagacctcg tccctgcccg ccaagcttgc
     2521 cgactgccgg tccaccgacg tcgagcgttc cgagctgttc atcgtcgagg gcgacagcgc
     2581 gctggggacg gccaagctgg cccggtcgtc ggaccaccag gccctgctgc cgatccgcgg
     2641 caagatcctc aacgtccaga aggcgtcggt ctcggacatg ctcaagaacg ccgaatgtgc
     2701 ggcgatcatc caggtcatcg gggccgggtc ggggcgttcc ttcgacctgg agtccgcccg
     2761 ctacggcaag gtcatcatca tgaccgacgc cgatgttgac ggcgcgcaca tccggaccct
     2821 gctgctgacg ctcttcttcc gctacatgcg tccgctcgtc gacgccgggc gggtctttgc
     2881 ggccgtaccg ccgctgcacc ggatcgaggt cgtcaacgcg gggtcggcca ggaacgagct
     2941 gatctacacc tacagcgagc cccagctgcg ccagaccctg agctcgctgg agaagcgcgg
     3001 caagaaggtc aagcagccgc tgcagcgcta caaggggctc ggcgagatgg acgccgacca
     3061 gctggccgag acgacgatgg acccgcgtca ccgcacgttg cgccgggtga cgctgcgaga
     3121 tgccgagatc gccgagcagg tgttcgacct gctcatgggc aacgacgtcg cgccccgcaa
     3181 ggacttcatc atcgactcgg cggccgagct ggaccgcgac cgcatcgacg cctgaggcgc
     3241 gtcagccttg ttcggcgatg agcgccgaca ggacgggccc gctgcccacc ggcagtgaca
     3301 ccgggatcgg gtcggtgatg gat
//
LOCUS       DATAIX010000091         1881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_210659, whole genome shotgun sequence.
ACCESSION   DATAIX010000091 DATAIX010000000
VERSION     DATAIX010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210659"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VHM65_01440"
     CDS             complement(<1..706)
                     /locus_tag="VHM65_01440"
                     /inference="COORDINATES: protein motif:HMM:NF015629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HEX2704394.1"
                     /translation="MDAPLDLDSDRRGLAGATARRDGAAVVALLSGRDPGLLLQHAGT
                     GLLVALTQQAEGATGLVAGLVAALTERDWCGDDELAGHLSAAAGGPPTGRVLVPADLD
                     MVADLLEGDLAYGFGGHLDRTTGEAWPEAAFEDGAADEDLDLDDDQRWLRVPNLGSRD
                     GWRDMHAFATGMVTEPDLTERLLDAIEGRGAFSRFRRVMDGHPGQLPSWHAFRDERRA
                     GRARDWLAGTGYDASPP"
     gene            complement(911..983)
                     /locus_tag="VHM65_01445"
     tRNA            complement(911..983)
                     /locus_tag="VHM65_01445"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(948..950),aa:Ala,seq:ggc)
     gene            complement(1024..1725)
                     /locus_tag="VHM65_01450"
     CDS             complement(1024..1725)
                     /locus_tag="VHM65_01450"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX2704395.1"
                     /translation="MTGRSPAVRHTRRVVVLRHGETTDNAANIWQGHRDSELSERGLA
                     QAAAAAPVLACYRPALIVSSDLSRAARTATALAAVTGLPVRLDERLREVHVGQWQGRS
                     GPDIHAEYPELVTALGRGEDIRRGVTGETVADVAVRVGAALDDVVASLGPGETAIVST
                     HGVASRAAVAGLCGIGQMEAFSWLQGLGNCHWAVVVESRPGLASGGATPWRIEGWNLS
                     ATVAVGDLGGPGASG"
BASE COUNT          266 a          710 c          672 g          233 t
ORIGIN      
        1 cgggtgggct ggcgtcgtac cccgtgccgg ccagccagtc tcgggcgcgg ccggcgcggc
       61 gctcgtcgcg aaaggcatgc cagctgggaa gctggccggg gtgcccgtcc atcacccggc
      121 ggaagcggga gaacgcgccg cggccctcga tggcgtccag cagccgctcg gtgaggtcgg
      181 gttcggtgac catgccggtc gcgaaggcgt gcatgtcgcg ccacccgtcg cgcgacccga
      241 ggttgggcac cctcagccag cgctggtcgt cgtccaggtc gaggtcctcg tcggcagcac
      301 cgtcctcgaa ggcggcctcc ggccacgcct caccggtggt ccggtccagg tggccgccga
      361 acccgtaggc gagatcgccc tcgagcaggt ctgccaccat gtccagatcc gccgggacga
      421 gcacgcgccc ggtgggcggg cccccggcgg ccgccgacag gtgcccggcg agctcgtcgt
      481 cgccgcacca gtcccgttcg gtcagggcgg ccaccagccc ggcgacgagg ccggtggcgc
      541 cctcggcctg ctgggtcagg gccaccagca gaccggtccc ggcgtgctgg agcagcagtc
      601 ccgggtcgcg tccgctcagc agggcaacga cggccgcacc gtcccgccgg gcggtggcac
      661 cggccaaacc ccgccgatcg gagtccaggt ccaggggtgc gtccatacag gtaccctccc
      721 tcgttcacca cggctcatca agtccgcgac gatcgggtcg aaggcaccct ggactggccg
      781 gcccgaggcc gctgcaccct ggccgaggtc ctgatgtggg aaggcatccc cgtcctgcac
      841 acgggagatc cgcacgatga tcggcggcgc cagtcgccgc gcctgccgcg cgccgaacgg
      901 ccagacccgg tggagctgag gggattcgaa cccctgaccc cctccatgcc atggaggtgc
      961 gctaccaact gcgctacagc cccgagcgcg atgctcgcgt acccccgaag ggactgcgga
     1021 agtctagccc gaggcgcccg ggccccccaa atcgcccacg gccacggttg ccgagaggtt
     1081 ccagccctcg atgcgccacg gcgtcgcgcc gccgctggcc aggcccggcc gcgactcgac
     1141 gacgacagcc cagtggcagt tgcccaggcc ctggagccag gagaaggctt ccatctggcc
     1201 gatcccgcac agcccggcga ccgccgcgcg gctggccacc ccgtgggtgg acacgatggc
     1261 ggtctccccc ggcccgaggg aggcgacgac gtcgtccagg gccgccccga cgcgcacggc
     1321 cacgtcggca accgtctccc ccgtcacacc gcggcggatg tcctcgccgc gcccgagcgc
     1381 ggtcaccaat tcggggtact ccgcgtggat gtccgggccg gagcggccct gccactggcc
     1441 gacgtggacc tcccggagcc gctcgtcgag gcgcaccggc aggccggtca cggccgccag
     1501 agccgtcgcc gtccgggcgg cccgggacag gtcgctgctc acgatgagcg ccggcctgta
     1561 acacgccagc accggcgcag ccgcggccgc ctgcgcgagc ccgcgttcgg acagctcgga
     1621 gtcccggtgg ccctgccaga tgttggccgc gttgtcggtc gtctcaccat ggcgcaggac
     1681 cacgacccgg cgggtatgac gaaccgcagg tgagcggccc gtcatacctg ctcgtcgggt
     1741 gcggcagagg gaacggacac cgccgaacgg gcagccgaaa gggggtcgcc gatcgcggcg
     1801 aggtcgacct gggggcagtc tttccagagt cgttcgaggg cgtagtacat gcgctcgtca
     1861 tcgtgctgga catgcacgac g
//
LOCUS       DATAIX010000092         1729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_181056, whole genome shotgun sequence.
ACCESSION   DATAIX010000092 DATAIX010000000
VERSION     DATAIX010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181056"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VHM65_01455"
     CDS             complement(<1..872)
                     /locus_tag="VHM65_01455"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX2704396.1"
                     /translation="MLTQLWDRIVGTPLTILAIALGAVLLRWLVHRLIARVVQASLSR
                     SDGRRTAFPRRTGRVFAEATGMVHERHVQRASTMGSILRSITTASVYAVALLTVMDLL
                     GIPLAPLLASAGVGGVALGFGAQSLVKDFLSGIFMIVEDQYGVGDVIDTGEAIGTVEE
                     VSLRVTRLRDGAGVIWYVRNGEIVRVANRSQGWSTATVDIPVAYGEDLEKVLALLRSV
                     VARLDGDDAWTDKLIDPPEVMGVESMAGGVVTIRIVAKCPPNEPFGVQREIRERVKAA
                     FDAAGISGPAFPPGV"
     gene            977..>1729
                     /locus_tag="VHM65_01460"
     CDS             977..>1729
                     /locus_tag="VHM65_01460"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF045011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX2704397.1"
                     /translation="MTSTPSDALTRLAQSYGVATDWWDWQGVHHIVSAEATRAVLAAF
                     DVDASDDAAAWRALESRHAEDWRRMVPPVVVCREGWTPWVPVHLPHGDPVVAFLELES
                     GQRWEVRQVDHLVEPRHVDGALVGEATFELPGNLPLGWHVLHTRSGVRAASATVVVTP
                     ARLELDPSLSSRRAWGLMSQVYAMRSARSWGVGDLADLAEIGSWAGAELGADFVLVNP
                     LHAAEAVAPMEPSPYLPTSRRFVNPIYLRVEDI"
BASE COUNT          246 a          622 c          612 g          249 t
ORIGIN      
        1 acgccggggg ggaaggccgg acccgagata ccggcggcgt cgaaggcagc cttgacccgc
       61 tcgcggatct cccgctggac gccaaaaggc tcgttcgggg ggcacttggc cacgatccgg
      121 atggtcacca cgccgccggc catggactcg acacccatca cctcgggtgg gtcgatgagc
      181 ttgtcggtcc aggcgtcgtc gccgtcgagc cgggcgacga ccgagcgcag cagcgccagg
      241 accttctcga ggtcctcgcc ataggccacg gggatgtcga ccgtcgccgt cgaccaccct
      301 tgggaccggt tggcgacgcg gacgatctcg ccgttgcgga cgtaccagat caccccggct
      361 ccgtcgcgca ggcgggtcac ccgcagcgag acctcctcga ccgtgccgat ggcctcgccg
      421 gtgtcgatga cgtcgccgac gccgtactgg tcctcgacga tcatgaagat gcccgagagg
      481 aagtccttga ccaggctctg cgcgccgaac ccgagggcga caccgccgac gccggcggac
      541 gcgagcagcg gggcgagcgg gatcccgagc aggtccatga ccgtcaggag cgcaacggcg
      601 tagaccgagg cggtcgtgat gctgcgcagg atcgagccca tcgtcgaggc ccgctggaca
      661 tgacgctcgt ggaccatgcc ggtggcctcg gcgaacaccc ggccggtccg gcgggggaag
      721 gcggtccgcc ggccgtcact acggctcagg ctcgcctgga cgacccgggc gatgagccga
      781 tgaacaagcc agcgcagcag cacggccccg agggcgattg ccaggatggt cagaggcgtt
      841 ccgacgatcc ggtcccacag ctgggtcagc atactcacct ggttctcact cgagtcctcc
      901 cattgtcggc gaacccggtg tccccggcac cggatgtcgg cgcgaggaag gcgcgtcggc
      961 atggaacact acccgcgtga cgagcacccc ctccgacgcg ctgacccggc tggcccagtc
     1021 ctacggtgtg gcgaccgact ggtgggactg gcagggcgtc caccacatcg tcagtgccga
     1081 ggcgacccgc gcggttctcg cggccttcga cgtcgatgcc tccgacgacg ccgccgcatg
     1141 gcgcgccctc gagtcgcgcc acgccgagga ctggcggcgc atggtgccgc ccgtggtggt
     1201 atgccgcgag ggctggaccc cgtgggtccc ggtccaccta ccccacggtg acccggtcgt
     1261 ggccttcctc gagctcgagt cgggccagcg ctgggaggtc cgtcaggtcg accacctcgt
     1321 cgagccgcgc cacgtcgatg gggccctcgt cggcgaggcg accttcgagc tgccgggcaa
     1381 cctgccgctg gggtggcatg tcctgcacac ccggtcgggg gtgcgcgcgg cctcggcgac
     1441 ggtggtcgtc acgccagcgc gcctcgagct cgacccgtcg ctgtcgagcc gccgggcctg
     1501 gggcctcatg tcccaggtct acgccatgcg ctcggcccgg tcgtggggtg tgggtgacct
     1561 cgccgacctc gcggagatcg ggtcgtgggc cggggccgag ctcggtgccg acttcgtcct
     1621 ggtcaacccc ctgcatgcgg ctgaggcggt cgcgccgatg gagccctcgc cctacctgcc
     1681 gacctcgcgg cggttcgtca acccgatcta cctccgcgtc gaggacatc
//
LOCUS       DATAIX010000093         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_240642, whole genome shotgun sequence.
ACCESSION   DATAIX010000093 DATAIX010000000
VERSION     DATAIX010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240642"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VHM65_01465"
     CDS             <1..558
                     /locus_tag="VHM65_01465"
                     /inference="COORDINATES: protein motif:HMM:NF022216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX2704398.1"
                     /translation="VVVLRQRGSVLAGWLGMLVLGGVLVGLLVSGSLWSSLGLTVLLV
                     LLLGLTWVLVLRPAVAIEPRGVVLHNPFRRTYLPWEVIEGTGSRWMLEVHTAQRRYAS
                     WAISSSLTRPSQWVIESYRRGPGERTSRVTAANASALIDEHREAYAAAQHPAPKAAVV
                     QTAWNRLELLAVGLPAVLLALTLLI"
     gene            1256..>1606
                     /locus_tag="VHM65_01470"
     CDS             1256..>1606
                     /locus_tag="VHM65_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704399.1"
                     /translation="MSTTTTHPRSALSDLRALKGASLARAAQLVGMTAERVEAIEAGD
                     GTGQEQAALYLALVDLPNHPADEDDYTPGRWESARWHAGLHLANLGERLRLAGARLCG
                     DQPRTVDQLMRGSYD"
BASE COUNT          181 a          543 c          619 g          263 t
ORIGIN      
        1 gtcgtcgtgc ttcgccaacg cggctccgtc ctcgcgggct ggctcgggat gctcgtcctc
       61 ggcggcgtcc tcgtggggct gctcgtgtcc gggtcgctct ggtcgtccct gggcctgacc
      121 gtcctgttgg tgctcctgct cggcctgacc tgggtgctcg tgctccgtcc cgcagttgcc
      181 atcgagcctc gcggggtggt cttgcacaac ccctttcgtc gcacctacct gccctgggag
      241 gtcatcgagg gcaccgggtc gcggtggatg ctcgaggtgc acacggcgca gcggcgctac
      301 gccagctggg cgatctcatc gagcctgacc cgcccgtcgc agtgggtcat cgagagctac
      361 cgacgggggc cgggggagcg gacctcccgg gtgacggcgg cgaacgcctc ggcgctcatc
      421 gacgagcacc gggaggcgta tgccgcggcg cagcaccccg cgccgaaggc cgccgtggtc
      481 cagaccgcct ggaaccggct ggagctgctg gcggtcggcc tccccgccgt gcttctcgcc
      541 ctcaccttgc tcatctgagg cgccactgac cggtcgccgt cgtcgtcgtc gtcgtgtcgt
      601 gaccgcaggg cgtgttcagg ctgcgcgccc gggggcgcgg aggtttggtc tcgtcgcgac
      661 ggttgcagtc gtcgtgtggt gaccgcagtg cgtgttcaca gtgtgctctc gcctgggcgc
      721 gcaggctcgc cgagtttggg ctggtggccg tcgtcgcgcg tctggccggc agggtggctg
      781 ggtggctgcg tgcggtcagg cggggatcga gaagggccag tcgggtgggt ctgggtcggg
      841 gcccgcagga gctcggtcta cccggatcga ctcagcatga ccgggtggcg caagtggtcc
      901 cgacccccgc tggggcgccg gtgagtcctg ccgctggggt ccaccgggtg gcccgggtga
      961 gtcctgccgc tcgggtccac ccagcggatc ggatgcccca ggtggtgccg tctcgggggg
     1021 tggggtgcct cgcccgtgga gcgtctggtc gtatgagcca ggggcggtgt cccagtggac
     1081 gccggtgggc gtgacgtgtg cggcgaggcg gtcacggtgc acgacgctgt gatggcgccc
     1141 gcacaggaga gcggcggtgt cgatgtcggt aagcccgccg tgggcccagt ggatggtgtg
     1201 gtgagtcccc gggtctgatg ttggcccgag ggacgtaacc cggaaggcaa ccaccatgag
     1261 caccaccacc acgcatcccc gctcggcgct gtcggacctg cgggcgctca aaggcgccag
     1321 cctcgcccgg gccgcgcagc tcgtcggcat gacggccgag cgggtcgagg cgatcgaggc
     1381 gggcgacggg accggccagg agcaggcggc cctatacctc gccctcgtcg acctacccaa
     1441 ccacccggcc gacgaggacg actacacgcc gggccgttgg gagtcggccc ggtggcacgc
     1501 cggcctgcac ctggccaact tgggcgagcg gctgcggctg gccggggccc gcctgtgcgg
     1561 cgaccagccg cgcacagtcg accagctcat gcgcggctcg tatgac
//
LOCUS       DATAIX010000094         3415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_35137, whole genome shotgun sequence.
ACCESSION   DATAIX010000094 DATAIX010000000
VERSION     DATAIX010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35137"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VHM65_01475"
     CDS             complement(<1..586)
                     /locus_tag="VHM65_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX2704400.1"
                     /translation="MARRRASVTRGRPGWGRRGLGLGAVGLLVVVGAVAARAASAPTA
                     SAASYRTATAEKRAVEQRLVLTGTVARVAQKTAVFPMSATVTNVAVGVGDTVTAGQPL
                     ASVDRAPLTSELLDAEAQVAQAKATVESDQSSSASSTSTLAARSSGSSGSGAGTAALL
                     RPVTLAMQAQERSCARVFGTTGGAVEGWVVDDEVG"
     gene            complement(579..1457)
                     /locus_tag="VHM65_01480"
     CDS             complement(579..1457)
                     /locus_tag="VHM65_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704401.1"
                     /translation="MTTSTRLARAASLIAAVGLLASACASSPSGSGSTPAATLGRTVP
                     GGAGAGAGLRQPGVGGLIAEIAGTTMQVQGDNTQPAVTWTAKTAISRVVTTSASAVKV
                     GVCVTVREPATLGTSNGSSASTATSSDPVTAASVQVSASVNGQCRPPGVRPSGASSGL
                     ATGTMTAGPGAGGQGTAGPGGPGWTGCGVTGRVDAVSGGTFVVERTAFAGASQTGATA
                     ATVSVRTTAATTYTTTVPGSARDLVVGRCVTASGTPDETGTVTASALTVRDAVSGACA
                     TAGVGGRQNAAGGFRG"
     gene            complement(1465..2694)
                     /locus_tag="VHM65_01485"
     CDS             complement(1465..2694)
                     /locus_tag="VHM65_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2704402.1"
                     /translation="MTWRDTVRTALEAIRVRRLRSGLTVLGILIGIAAVILTVGLGQG
                     AQAKVADQINALGSNLLVISPGSSTSVGGIRGGRGTATTLTSADAAILANTDVAPDIA
                     AVAPTSTSSQSLIAGTTTWTSSVIGTTPSWLSVRARSVSAGRFLTGEDVSTGAMVAVL
                     GSTTASELYAGRDPLGQSVTINGQAYTVVGVLTAVGSGSVLDEDDHVVVPRTTFATRI
                     STSSTPDSVSSIYVEAQDQDSLSAAYQEATAALTTAHGVTASTVDFTVSSQQSLVETA
                     TSTAAILTVLLASIAAISLLVGGIGVMNIMLVSVTERIREIGLRKALGATPAMILRQF
                     LVEASILGLVGGVFGLGLGVIGALALPGLLDQPVAISPVAAAAALAVSLVIGVAAGVY
                     PASRAARLTPIDALRSE"
     gene            complement(2691..>3415)
                     /locus_tag="VHM65_01490"
     CDS             complement(2691..>3415)
                     /locus_tag="VHM65_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2704403.1"
                     /translation="PGPSAPPGPIPAILDMRHVTKTYAAGSLEVTALAGITLSVPEGT
                     FLAIMGPSESGKSTLMHILGCLDVPTSGQYLLAGQDVSQLDEAALAQIRNERIGFVFQ
                     QFNLLATMTAWRNVELPLSYAGVPRAERRERALAALAMVGLADRVDHRPGELSGGQQQ
                     RVSIARALVTDPDLILADEPTGNLDSASTADILGLFRTLHGQGRTIVIITHERDIAEA
                     VGSTVTLRDGRVAAPAAPGVSR"
BASE COUNT          490 a         1224 c         1245 g          456 t
ORIGIN      
        1 gccctacctc gtcatcgacg acccagccct cgacggcccc gcccgtggtg ccgaacacgc
       61 gcgcacagct acgttcctgc gcctgcatcg ccagggtcac cgggcgcagc agcgcggccg
      121 tcccagcgcc ggacccggac gacccggagg acctcgcggc tagggtggaa gtcgagctcg
      181 cgctcgagga ctggtccgac tccaccgtgg ccttggcctg cgcgacctgc gcctcggcat
      241 cgagcagctc ggaggtcagc ggtgcccggt ccacgctcgc cagcggctgc cccgccgtga
      301 ccgtgtcacc gaccccgacc gcgacgttcg tgaccgtcgc gctcatcggg aagaccgcgg
      361 tcttctgcgc gacccgcgca accgtgccgg tgagaacgag ccgctgctcg accgcccgct
      421 tctccgcggt cgccgtgcgg taggacgccg ccgaggcggt cggtgccgac gcggctctgg
      481 ccgccacggc gccaaccacc acgagcaggc caacggcacc gaggccgagg ccgcggcgcc
      541 cccacccggg tcgcccgcga gtgaccgagg cccgccgcct agccacggaa gccacccgcc
      601 gcgttctggc ggcccccgac gccggccgtg gcgcacgcac ccgagacggc gtctcgcacc
      661 gtcagtgccg aggctgtcac ggtcccggtc tcgtccgggg tccccgaggc cgtcacgcac
      721 cggcccacga cgagatcgcg agccgatccc ggcacggtcg tcgtgtacgt cgtcgcggcg
      781 gtggtccgga ccgacaccgt cgcagccgta gcgccggtct gtgatgcgcc ggcgaaggct
      841 gtccgctcca ccacgaaggt ccccccgctg acggcgtcaa cccggccggt cacgccgcag
      901 cccgtccagc ctggcccgcc tggtccggcc gtgccctggc cgccagcgcc agggccggcg
      961 gtcatcgtcc ccgtcgccag ccctgaggag gcgccggacg gacggacgcc ggggggcctg
     1021 cactggccgt tcacggaggc gctgacctgg acactggccg cagtcaccgg atcggagctc
     1081 gtggctgtgc tcgcggaaga accgttcgac gtgcctagcg tggctggctc gcggaccgtg
     1141 acacagacgc cgaccttgac agccgacgcc gacgtcgtga cgacccggct gatcgcggtc
     1201 ttggccgtcc aggtcaccgc gggctgggtg ttgtcgccct ggacctgcat cgtcgtcccg
     1261 gcgatctccg cgatgaggcc gccgactccg ggctggcgca agccggcgcc agcgccggcg
     1321 ccccccggga cggtccggcc gagcgttgcc gcgggcgtgc tgcccgatcc tgacggcgac
     1381 gaggcgcagg ccgatgccag cagcccgacg gcggcgatca gtgaagcagc acgggcaagg
     1441 cgggtcgatg tggtcatgag cttcctactc gctgcgaagg gcgtcgatgg gggtcagccg
     1501 ggcggcgcgg ctggcggggt agacgccagc ggcgaccccg atgacgagag acacggccag
     1561 cgcggccgcc gcagcgaccg gggagatcgc gacgggctgg tcgagcagac ccggcagcgc
     1621 gagcgctccg atcactccga gaccgaggcc gaacaccccc ccgacgagcc cgaggatgct
     1681 cgcctcgacg aggaactggc gcaagatcat ggcaggggtg gcaccgagcg ccttgcgcag
     1741 gccgatctcg cggatccgct ccgtgaccga cacgagcatg atgttcatga cgccgatccc
     1801 accgacgagc agcgagatgg cggcgatgct cgccaggagc accgtgagga tcgcggcggt
     1861 gctggtcgcg gtctcgacca gcgactgctg gctgctcacg gtgaagtcca cggtggaagc
     1921 cgtgacgccg tgagcggtcg tcagggctgc cgtggcttcc tggtatgccg cggacaggct
     1981 gtcctggtcc tgggcttcga cgtagatcga ggacacgctg tccggagtgc tgctggtgga
     2041 gatccgggtc gcgaacgtcg tccgaggcac gacgacatgg tcgtcctcgt cgagcaccga
     2101 ccccgagccg accgcggtga ggaccccgac gaccgtgtat gcctggccgt tgatcgtcac
     2161 ggactgaccg agcgggtcgc gcccggcata cagctcgctg gccgtcgtgg acccgaggac
     2221 cgcgaccatg gcacctgtgg agacatcctc gccggtgagg aaccggccgg cgctcacgct
     2281 gcgcgcccga accgaaagcc aggatggcgt cgtaccgatg accgacgacg tccaggtcgt
     2341 cgtcccggcg atgagggact gggaggaggt cgaggtcgga gccacggcgg cgatgtccgg
     2401 ggcgacatcg gtgttggcca ggatcgcggc atcggccgag gtcagggtcg tcgccgtccc
     2461 gcgcccgccc cggatgccac cgacgctggt ggagctgccg ggcgagatca cgagcaggtt
     2521 ggaccccagg gcgttgatct ggtcggcgac cttggcctgg gctccctggc ccaggcccac
     2581 ggtgaggatg acggcggcga tcccgatcag gatgccgagc accgtcagcc ccgaccgcag
     2641 gcgtcggacg cggatcgcct cgagggccgt gcggacggtg tcgcgccagg tcaccgtgac
     2701 acccccggtg ccgcaggcgc cgcgacgcgt ccatcgcgca aggtcactgt cgaccccaca
     2761 gcctcggcga tgtcgcgctc gtgggtgatg atgacgatgg tccggccctg gccgtgcagc
     2821 gttctgaaca gccccaggat gtccgccgtc gaggccgagt cgaggttgcc cgtcggctcg
     2881 tccgcgagga tgaggtccgg gtcggtgacc agggcccggg cgatcgacac ccgctgttgc
     2941 tggccgccgg acagctcccc cggacggtgg tcgacgcggt cggccagccc gaccatcgcg
     3001 agtgccgcca gcgcccgttc ccggcgctcg gcccgcggca ccccggcgta gctgagcggc
     3061 agctcgacgt tgcgccaggc agtcatcgtc gccagcaggt tgaactgctg gaagacgaag
     3121 ccgatgcgct cgttgcggat ctgcgccagc gccgcctcgt cgagctggct gacgtcctgg
     3181 cccgcgagca ggtactgacc ggaggtgggg acgtcaaggc agccgaggat gtgcatgaga
     3241 gtcgacttgc ccgactcgga gggtcccatg atcgccagga aggttccttc cgggacgctc
     3301 agcgtgatcc cggcgagggc ggtcacctcc agcgagccgg cggcgtaggt cttggtgaca
     3361 tggcgcatgt cgaggattgc ggggatcggc cccgggggag ccgacggtcc tggcg
//
LOCUS       DATAIX010000095         4532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_94633, whole genome shotgun sequence.
ACCESSION   DATAIX010000095 DATAIX010000000
VERSION     DATAIX010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94633"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /locus_tag="VHM65_01495"
     CDS             <1..1456
                     /locus_tag="VHM65_01495"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX2704404.1"
                     /translation="RWTQWIFLQIFSSWYDVTAVRADGGLGMARPITDLVGELESGSR
                     PTPDGTAHALPDSMLPLELPDVPDYSPKTFDPEDGASQPEPPLSRAGEWVNVDLDLAD
                     GRGVQRYRREANTMPNWAGSCWYYLRYLDPANSDALVDPANERYWIGPHEGAIPGAPE
                     GSRDPGGVDLYVGGVEHAVLHLLYARFWHKVLFDLGHVCSEEPFRTYFSQGYIQAPAY
                     TDARGQYVEAGEVLEHPGSHGADPTFTWEGQPVRREFGKIGKSLKNMVSPDEMYEAYG
                     ADTFRVYEMSMGPLDLSKPWETRAVVGALRFLQRLWRNVVHEVTGEVQVSDIAPDAAT
                     LTALHKTIAGVREDYDALRFNTAIAKLIELNNALTRLAEVPRAVVEALVLMVAPVAPH
                     IAEELWARLGHSDGLTYVPFPVADRSLVVDELVTCVIQINGKVRDRIEVPATISEDDL
                     RERALARDRVVAAAAGGIRAVIVRAPKIVSVVTA"
     gene            1506..2369
                     /locus_tag="VHM65_01500"
     CDS             1506..2369
                     /locus_tag="VHM65_01500"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX2704405.1"
                     /translation="MNVAVVTDSTAYLPPELAREHGIRVVPLHVFVAGHERREGIDIT
                     LADLSEALRQFRPVTTSRPAPQVFLDLYEELAAAGADAVVSLHISAEMSGTVQSAQLA
                     AAQAPVPVHVVDSRSIGMAMGFAAVSAAQAASGGADAVAVAEVARARSAAAHVVFYVD
                     SLEHLQRGGRIGLASALLGSALAIKPILAVLDGRIVARDRVRTTGKALARLVELSAAA
                     ADAAERAGQVVDIAVQHLDSVDRAEQLVTTLRERVPGAGEIRLGELGAVVGAHVGPGT
                     LAITVSPRAAS"
     gene            2366..3016
                     /locus_tag="VHM65_01505"
     CDS             2366..3016
                     /locus_tag="VHM65_01505"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX2704406.1"
                     /translation="MSGVTAGPVSLTSDALLTFVLAVATVLLVSFFIGSVNPAAIFAR
                     MLGKSLATGSGNPGATNAGRVLGLRWGVVVGLLDVLKGFVPVWVCATTFGPLVAYTAG
                     IAAVLGHIFSPFLRGRGGKGVATALGVVLGVHPVLVLVVVLVFGIGVLLGRQVAAGSV
                     AAALALLVFGATSFLGVTGDGPLTGVWAVTLGVIVVTRHTPNIRGWLARAGPSPTD"
     gene            3768..4460
                     /locus_tag="VHM65_01510"
     CDS             3768..4460
                     /locus_tag="VHM65_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX2704407.1"
                     /translation="MARPRVVSVPGPLRHARVRTPRRTGLALAAVSLAALVLFGARVW
                     LAQSAAQPRPVPAAVATSGEGTPNGLVGRSVPPAFAPGTSAASTTKERLVVHVVGSIA
                     HPGIVRVPAGSRVIDAITAAGGPTRHADLSRVNLARLVVDGEQIVVPRPGEVVAAPPG
                     ATPSGAGARVDLNTADLTALDALPGVGPVLAQRILDWRAEHGRFSTVDELGEVSGIGD
                     KLLAQLRPKVQV"
BASE COUNT          552 a         1626 c         1648 g          706 t
ORIGIN      
        1 ccgctggacc cagtggatct tcctgcagat cttcagctcc tggtatgacg tcaccgccgt
       61 ccgcgcggac ggcgggctcg gcatggcgcg ccccatcacg gatctggtgg gggagctcga
      121 gtcgggatcg cgtccgacgc cggacgggac tgcccacgcc ctgcccgact cgatgctgcc
      181 gctcgagctg cccgacgtgc ccgactactc gcccaagacc ttcgaccccg aggatggcgc
      241 gagccagccg gagccgccgc tgtcccgggc cggcgagtgg gtcaacgtcg acctcgacct
      301 ggccgacggg cgtggcgtcc agcgctacag gcgcgaggcc aacacgatgc ccaactgggc
      361 cgggtcctgt tggtactacc tgcgctacct cgacccggcg aactccgacg ccctcgtcga
      421 cccggccaac gagcggtact ggatcggccc tcacgagggc gccatccccg gggcgcccga
      481 gggcagccgc gaccccggcg gcgtcgacct ctacgtcggt ggggtggagc acgccgtgct
      541 gcacctgctc tatgcccggt tctggcacaa ggtgctcttc gacctcgggc acgtctgctc
      601 ggaggagccg ttccggacct acttctcgca gggctacatc caggcgccgg cctacaccga
      661 cgcgcgcggg cagtacgtcg aggccgggga ggtgctcgag caccccgggt cgcacggcgc
      721 cgacccgacg ttcacctggg agggtcagcc ggtcaggcgc gagttcggca agatcggcaa
      781 gtcgctcaag aacatggtga gcccggacga gatgtacgag gcgtacggcg ccgacacgtt
      841 ccgggtctac gagatgtcga tgggcccgct ggacctgtcc aagccgtggg agaccagggc
      901 cgtcgtcgga gcactgcgct tcctgcaacg gctgtggcgc aacgtggtcc acgaggtcac
      961 cggagaggtg caggtcagcg acatcgcccc ggacgcggcc acgctgacgg cgctgcacaa
     1021 gacgattgcc ggggtgcgcg aggactacga cgcgctgcgc ttcaacacgg ccatcgccaa
     1081 gctcatcgag ctcaacaacg ccctgaccag gctcgccgag gtgccgcggg cggtcgtcga
     1141 ggccctcgtc ctcatggtcg cgcctgtagc gccgcacatc gccgaggagc tgtgggcccg
     1201 gctcggacac tcggatggcc tcacctatgt gcccttcccg gtggccgacc ggtcgctcgt
     1261 cgtcgacgag ctcgtcacct gcgtgatcca gatcaacggc aaggtgcgcg accgcatcga
     1321 ggtgcccgcg acgatctccg aggacgacct gcgcgagcgt gccctggccc gcgaccgggt
     1381 cgtggccgca gctgcgggcg gcatccgcgc ggtcatcgtc cgcgccccga agatcgtcag
     1441 cgtcgtcacc gcgtagccac gcgagtgcag gtccgcgtca taccctcgcg cggtagcctg
     1501 cgcgggtgaa cgttgccgtc gtcaccgact ccacggcata cctgccaccg gagctcgccc
     1561 gcgagcacgg gatccgggtc gtgccgcttc atgtcttcgt ggccggtcac gagcgccgcg
     1621 aaggcatcga catcacgctg gccgacctgt ccgaggcgct gcgccagttc cggccggtga
     1681 cgacctcgcg cccggcgccg caggtgttcc tcgacctcta cgaggagctc gccgcggctg
     1741 gtgccgacgc ggtcgtgtcg ctgcacatct cggccgagat gtcagggacg gtgcagtccg
     1801 cccagctcgc ggccgcgcag gctcccgtcc cggtccacgt cgtggactcg cgctcgatcg
     1861 gtatggcgat gggtttcgcc gcggtgtctg cggcccaggc ggcgtcgggc ggcgccgatg
     1921 ccgtcgctgt cgccgaggtg gcccgggctc ggtcggccgc cgctcacgtc gtcttctatg
     1981 tcgacagcct cgagcacctg cagcggggag ggcggatcgg gttggcctcg gcgctgctcg
     2041 ggtcggcgct cgcgatcaag ccgatcctgg ctgtcctgga tgggcgcatc gtggcgcgtg
     2101 accgcgtccg gaccaccggc aaggcgctcg cccggctggt cgagctgtcc gccgccgccg
     2161 ccgatgcggc cgagcgggcc ggccaggtcg tcgacatcgc cgtccagcac ctcgactccg
     2221 tcgatcgggc cgagcagctc gtcacgaccc tgcgcgagcg ggtccccggg gcgggggaga
     2281 tccggctcgg cgagctcggc gccgtcgtcg gtgcccatgt cggaccgggc acgctggcga
     2341 tcaccgtgtc gccgcgggct gcgtcgtgag tggcgtgacc gccggtccgg tctccctcac
     2401 gtcggacgcg ctactcacct tcgtgctggc cgtcgccacc gtcctgctcg tgtcgttctt
     2461 catcgggtcg gtcaaccctg cggcgatctt cgcgcggatg ctcggcaaga gcctggccac
     2521 cgggtcgggc aacccgggcg cgaccaacgc cgggcgtgtg ctaggcctgc ggtggggggt
     2581 ggtggtcgga ctgctcgacg tcctcaaggg gttcgtcccc gtgtgggtgt gcgcgacgac
     2641 cttcgggccc ctcgtggcct acaccgccgg catcgccgcg gtcctcggcc acatcttctc
     2701 gccgttcctg cgcgggcgcg gcggcaaggg cgtcgcaacg gcgctcggcg tcgtcctcgg
     2761 ggtgcacccg gttctggtcc tggtcgttgt cctcgtgttc ggcatcggcg tgctgctcgg
     2821 gcgccaggtg gccgccgggt cggtggcggc ggcactggcg ctgctggtgt tcggcgcgac
     2881 ctcgttcctc ggcgtgaccg gagacggtcc gctcaccggc gtgtgggcgg tgaccctggg
     2941 ggtcatcgtg gtgacccgcc acaccccgaa catcaggggc tggctggccc gggccggtcc
     3001 ttcgccgacg gactgagagc gcgtctcgga gctctggtgc tggcggggtg cgtgtctgcc
     3061 tgtatggccg tgatgcgtgg ctgagctctc ggtgggtcag tggttgccga gactgggtcc
     3121 tgcctggagc taacggtgcc gtcatcactg acgtggatcc gggcggcctg caccggccag
     3181 atccacctca ctacccgcgc ggcccagcat cgaggccgat ccggccggcc agcaccggcc
     3241 agatccaccg cggtggccca gctccgtcag ccgcccttgg acctccgccc gcgatgagcg
     3301 tcgacgacac cgtccacccg ctgggcgtcc gcgggtacct cccagcgggt ccacgacgcg
     3361 cgatacgacc ggctgcgcgt gcgcgacggt ctgagagttc gccagtcggt tgtccacagc
     3421 ggtaggcgac ggagtcccac cgtccccagg cggcacgagg tgactcccgg ttggcgggag
     3481 gaccgaccta cggtgctggt atggcgcgtg ctcctgaccc agcgatcccg cgagcccgtc
     3541 tgcccgggtt gctcgggttg ctcggtcagc ctcgcctgtc tgccaggcct ggggtgggct
     3601 gggttccctc gcggccatcg gagcctgccg aggcgcctcc cgcgccggac gcgacggtgc
     3661 gcgacgagtg ggctctgcgg ccgtcgacgg tcgcaggagg ggtcgacccc gaggccggtg
     3721 gtggtccggc cgaggcaggg ctgcccacgc gacgcgaccg acggtcggtg gcgcgtccgc
     3781 gggtggtcag cgttcccggt ccgctgcgcc acgcgcgggt caggactccg cgtcgcaccg
     3841 ggctcgcgct cgccgcggtg tcgctggctg ccctggtgct cttcggggcc cgggtctggc
     3901 tcgcacagtc cgctgctcag ccgcggcccg tcccggcagc cgtcgctacc agcggcgagg
     3961 ggacacccaa tggcctggtc gggcggtccg tgccccccgc gttcgcgccg gggacgagtg
     4021 ctgcgtcgac caccaaggag cggctcgtcg tgcatgtcgt cgggtccatc gcccatccgg
     4081 ggatcgtgcg cgttcccgcg gggtcccggg tcatcgacgc catcaccgcg gcagggggcc
     4141 cgaccaggca cgctgacttg tccagggtca acctggcccg gctcgtcgtc gacggtgagc
     4201 agatcgtggt ccctcgaccc ggggaggtcg tcgcggcccc gccgggtgct acgccctcgg
     4261 gcgctggcgc tcgggtggac ctcaacacgg ctgacctgac tgccttggac gccttgcccg
     4321 gagtcgggcc ggtcctggcg cagcgcatcc tggactggcg agccgaacac gggcggttca
     4381 gcaccgtcga cgagctcggc gaggtcagcg ggatcgggga caagctgctc gcccagctcc
     4441 gtcccaaggt ccaggtatga cgtcgaaggc tcgccagggc gccgcaccgg ctcagccgga
     4501 gcacgcgcgg ggcggtcggg gagggagcgg ag
//
LOCUS       DATAIX010000096         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_210864, whole genome shotgun sequence.
ACCESSION   DATAIX010000096 DATAIX010000000
VERSION     DATAIX010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210864"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..972)
                     /locus_tag="VHM65_01515"
     CDS             complement(148..972)
                     /locus_tag="VHM65_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX2704408.1"
                     /translation="MSTLEGSPLVVTRAGGVATLWLNRPDKRNAVSYDMWLGLAARTQ
                     ELAADSSVRVLAIRGVGDHFCAGGDIGGLGELPFEEYRRANEQADRALAAFPKPSIAF
                     ITGACIGGGAEIAIACDLRLADTSARFGITPARLGIVYPGFAVTRAVQLIGESATKHL
                     LYTAEIIDAERALRIGLVDELLDPGLAVARLDELTVLMSQERSLLTQMASKEMVDSAV
                     EHGRVTDEVEARWDAALAASPDAAEGIAAFLEGRAPAFSWAPRPPASTTSLADTDG"
     gene            1103..1516
                     /locus_tag="VHM65_01520"
     CDS             1103..1516
                     /locus_tag="VHM65_01520"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2704409.1"
                     /translation="MTSRLNPYLSFSGNARAAMEFYTDVFGGELTVSTFGDYGQAETP
                     IADQIMHAMLVTDAGYALMGADTPPGMTHTPGDTITLSLSGDDSELLHGYWDKLSAGG
                     TVTTPLAIQMWGDEYGSCVDPYGVVWLVNISQQPG"
     regulatory      complement(1594..1666)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1724..>2368)
                     /locus_tag="VHM65_01525"
     CDS             complement(1724..>2368)
                     /locus_tag="VHM65_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2704410.1"
                     /translation="VVHLAAIPAPGLHSDVTTFRNNMLSTFNVFHAAVRLGIRTVVYA
                     SSETVLGLPFDTPPPYLPVDEDYPARPESVYLLVKHLDEQMAIELCRWHPDLTITALR
                     FSNVMDLEDYRAFPGFDADARRRKWNLWAYIDGRDGAQAVERALHRPGPGFDRFIIAS
                     PDTVMSRPNADLVAEVFPGVPVTRDLGEHDTMLSIDKARRVLGYQPEHTWRDHA"
BASE COUNT          363 a          823 c          827 g          355 t
ORIGIN      
        1 ctccacatag cgctgcccgc accgattgtg agctcggtgg ctgtcagtgg ccgtggcttg
       61 ctgaggtgga tccggccggc tgggaccggt cagcgccggt ctgacccacc tcgttggcca
      121 ggacgctggg tcgcctggtt accgtcgtca tccgtctgta tcggccaggg aggtcgtcga
      181 tgccgggggc cgcggagccc agctgaacgc cggggctcgc ccctcaagga acgccgcgat
      241 cccttcggcg gcgtcggggc tggccgcgag ggcggcatcc cagcgtgcct ccacctcgtc
      301 cgtcacccgc ccgtgctcca ctgcggagtc gaccatctcc ttgctcgcca tctgggtcag
      361 cagagagcgt tcctgcgaca tcaggacggt gagctcgtcg agccgcgcga ccgccagccc
      421 cggatcgagg agctcgtcga cgagaccgat ccgcagtgcc cgctcggcat cgatgatttc
      481 ggcggtgtag agcaggtgct tggtcgccga ctcgccgatc agctgcaccg cccgggtgac
      541 cgcgaagcca gggtagacga ttcccagccg ggccggcgtg atgccgaacc gcgcgctcgt
      601 gtcggccagc cgcaggtcgc acgcgatggc gatctccgcg cccccgccga tgcacgcgcc
      661 ggtgatgaag gcgatgctcg gcttggggaa ggccgcgagg gcccggtcgg cctgctcgtt
      721 tgccctgcgg tactcctcga acggaagctc gcccaggccg ccgatgtcgc cgccggcgca
      781 gaaatggtcc ccgacaccgc ggatggcgag cacccgcacc gagctgtcgg ccgcgagctc
      841 ctgcgtccgc gcggccagcc ccagccacat gtcgtagctc accgcgttgc gcttgtccgg
      901 ccggttgagc cacaacgtcg ccaccccacc cgcacgggtc acgaccagcg ggctcccctc
      961 gagcgtcgac atgggtcgag tatgccggtc ccgctggcaa gcacgtcgtc ccaccggctc
     1021 ttccgcgccc cgccgagtgc gggttaggct acccaaaccc gcgagccaac cggcggaccg
     1081 ccatacccgg aaaggacccg acatgacgtc gcgactcaac ccgtacctca gcttcagcgg
     1141 caacgcccgt gcggcgatgg agttctacac ggacgtgttc ggcggtgagc tgacggtgag
     1201 cacgttcggt gactacggcc aggccgagac accgatcgcc gatcagatca tgcacgcgat
     1261 gctcgtcacg gacgcgggct acgcgctcat gggcgctgac acaccgccgg gcatgacgca
     1321 cacgccgggc gacacgatca ccctcagcct gagcggcgac gacagcgagc tcctgcacgg
     1381 ctactgggac aagctctcgg ccggcgggac ggtcacgacg ccgcttgcca ttcagatgtg
     1441 gggcgacgag tacggctcgt gcgtcgaccc gtatggcgtc gtctggctcg tcaacatctc
     1501 ccaacaacct ggctgacgac agaccgccgc gcgcagggcg agttcggcgc tgacccggtc
     1561 gcaccacagc aggtcggccg ggtcggcagg cacaggagtc gggcttccgc gccatgcagc
     1621 acggttaccg ttgcgggtca gcgccggact ctcaccggtc ttccctccct cgaacgcagg
     1681 cagggtaagc acccccgccg gacggcgcgg accgcgcctt ccgtcaggcg tggtcgcgcc
     1741 aggtgtgctc cggctggtag ccgagcaccc gacgggcctt gtcgatcgaa agcatcgtgt
     1801 cgtgctcgcc gaggtcgcgg gtgaccggga ccccggggaa cacctcggcg acgaggtcgg
     1861 cgttgggccg tgacatcacg gtgtcgggcg aggcgatgat gaaccggtcg aagcccgggc
     1921 cggggcggtg cagcgcccgc tcgaccgcct gggcgccgtc gcggccgtcg atatacgccc
     1981 agaggttcca cttccgccgg cgggcgtccg cgtcgaagcc cgggaacgcg cggtagtcct
     2041 cgaggtccat gacgttggag aaccgcagtg ccgtgatcgt caggtcgggg tgccagcggc
     2101 acagctcgat cgccatctgc tcatccagat gcttgacgag caagtagacg ctctcggggc
     2161 gtgccgggta gtcctcgtcg acgggaaggt acggcggtgg cgtgtcgaag ggcagcccga
     2221 gcaccgtctc gctcgaggcg tagacgacgg tgcggatgcc gagccggacg gcggcatgga
     2281 agacgttgaa cgtcgagagc atgttgttgc ggaacgtcgt gacgtcgctg tgcaaccccg
     2341 gggcgggtat ggcggccagg tggacgac
//
LOCUS       DATAIX010000097         1547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_5097, whole genome shotgun sequence.
ACCESSION   DATAIX010000097 DATAIX010000000
VERSION     DATAIX010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5097"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VHM65_01530"
     CDS             <1..252
                     /locus_tag="VHM65_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704411.1"
                     /translation="PYDHADITAALANRVCLEALSASARRRQDARDGSTWDPARPLLH
                     GRIPHGRLGGPGSGAERHTHHHPAGPGHDPSTDLAPEEH"
     gene            256..>1547
                     /locus_tag="VHM65_01535"
     CDS             256..>1547
                     /locus_tag="VHM65_01535"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX2704412.1"
                     /translation="MAAVTEQALLASVPAGLLIGGTWRASSSGGRFGVEDPATGRTLT
                     TVADATVGDGKAALDAAVAAAPGWATVPPRDRGEILRRAFEVITARTEEFATLMTLEM
                     GKPLHEARAEVAYGAEFFRWFSEEAVRIHGRYSVAPNGATRLMTMKAPVGPTLMITPW
                     NFPLAMGTRKIGPAVAAGCTMVVKPAAQTPLSMLLLAQVMQECGLPDGVLNVITTHST
                     GAVMEPIIRDPRLRKLTFTGSTAVGRRLVEQSAEQLLRVSMELGGNAPFIVFEDADVD
                     RAVEGAMAAKMRNIGEACTAANRFFAHESVAQEFSAKFAARMGALVVGKGTVKGVDVG
                     PLIDDAARAGVDELVRDAVAKGATVLTGGGPIDGEGYFYAPTVLTDVIADARMMTEEI
                     FGPVAPVATFRTEDEVVSAANSTEYGLASYVFTRDLSR"
BASE COUNT          222 a          574 c          539 g          212 t
ORIGIN      
        1 ccctacgacc acgccgacat caccgctgcg ctggccaacc gcgtgtgcct cgaggcactc
       61 tcggcgagcg ctcgtcgtcg ccaggacgct cgcgacggct cgacgtggga cccggctcgc
      121 ccgctcctgc acgggcggat cccccacggg cgcctcggcg ggcccggcag cggtgcggaa
      181 cgccatactc atcaccaccc ggccggcccg ggccacgacc ccagcaccga tctcgcaccc
      241 gaggagcact gagccatggc agccgtcacc gagcaggcgc tgctcgcctc cgtcccggcg
      301 ggcctgctca tcggtgggac ctggcgggcg tcctcgagcg gcgggcggtt cggggtcgag
      361 gacccggcga ccggccgaac gctcacgacc gtcgccgacg ccaccgtcgg ggatggcaag
      421 gcggcactcg atgcggccgt ggccgctgcg ccgggctggg ccacggtgcc accgcgggac
      481 cgcggcgaga tcctgcgccg ggccttcgag gtcatcaccg cgcggaccga ggagttcgcg
      541 acgctcatga ccctcgagat gggcaagccg ctgcacgagg cgcgggccga ggtcgcgtat
      601 ggcgccgagt tcttccgctg gttctccgag gaggccgtgc ggatccacgg tcgttactcg
      661 gtcgccccga acggtgcgac ccggctcatg acgatgaagg ccccggtcgg gccgacgctc
      721 atgatcacgc cgtggaactt cccgctggcg atggggacac gcaagatcgg cccggccgtc
      781 gccgcggggt gcaccatggt cgtcaagccg gccgcgcaga cgcccctgtc gatgctgctc
      841 ctcgcccagg tcatgcagga gtgcgggctg cccgacggtg tcctcaacgt catcacgacg
      901 cacagcacgg gcgcggtcat ggagccgatc atccgtgacc cgcggctacg caagctgacc
      961 ttcaccgggt cgaccgcggt cgggaggcgc ctcgtcgagc agtcggcgga gcagctgctg
     1021 cgggtctcga tggagctcgg cggcaacgcg ccgttcatcg tcttcgagga cgccgacgtc
     1081 gaccgcgccg tcgaaggcgc catggccgcc aagatgcgca acatcggcga ggcctgcacg
     1141 gcggccaacc ggttcttcgc ccacgagtcc gtcgcgcagg agttctcggc caagttcgcc
     1201 gcccggatgg gcgccctggt cgtcggcaag ggcaccgtca agggcgtcga cgtcgggcca
     1261 ctcatcgacg acgcggcccg cgccggggtc gacgagctcg tgcgcgacgc cgtcgccaag
     1321 ggtgccacag tcctcaccgg cggtggcccc atcgacggcg agggctactt ctacgctccg
     1381 acggtcctga cggacgtcat cgccgacgcg cggatgatga ccgaggagat cttcggcccg
     1441 gtcgcccccg tcgcgacctt ccgcaccgag gacgaggtcg tctcggccgc caacagcacg
     1501 gagtacggtc tcgcgtcata cgtcttcacc cgtgacctgt cccggat
//
LOCUS       DATAIX010000098         1711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_121643, whole genome shotgun sequence.
ACCESSION   DATAIX010000098 DATAIX010000000
VERSION     DATAIX010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121643"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VHM65_01540"
     CDS             complement(<1..784)
                     /locus_tag="VHM65_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2704413.1"
                     /translation="MAPFDVVVIGGGVSGLAAAWQLTRAGRALSICVYDAAPVPGGKL
                     ASGVVAGHRIDIGAESMLARRPEAVGLVEEAGLAGGLTHPDVTAASVYSRGRLWPLPP
                     RTLMGIPSDPETTRGLLTDAEVARIYAEPAHPEPVSSDISVGDLVAGRLGTAVLDRLV
                     EPLLAGVYAGSARRISAAASLPAFYAAAASGHGLVATAAELARASATDPRPVFAGYAG
                     GLGLLAEHLAARLEAAGVEIRCGAHVEGLRPRPGGWSVSAISG"
     gene            937..>1711
                     /locus_tag="VHM65_01545"
     CDS             937..>1711
                     /locus_tag="VHM65_01545"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEX2704414.1"
                     /translation="MTNRIPLALGAGLATVMLALVGCSGDSATTSGAAPGGAEAAPAP
                     GAPARDGAVAGGDSKAGTTLVTAQKVIRNATVLLEVPDVAKAAATVRTIAAGAQGVVT
                     SEQLSSGSDVPKPQPADQVAPTRFADATIVISVPQSSLDRVLDRLSAIGTLTSRQLTS
                     QDVTATFVDTTSRVASMKASVERIRALMAKATSITDLASLEGELSRRQTDLEAMQSQL
                     KALEDQVAMSSVTVTMAGPGTTLREDTDWGFFNGLRNGLE"
BASE COUNT          270 a          631 c          608 g          202 t
ORIGIN      
        1 ctcccgagat ggcggagacc gaccaccctc ccggacgcgg ccgcagcccc tcgacgtgag
       61 cgccgcagcg gatctcgacc ccggctgcct ccaggcgtgc ggcgaggtgc tcggcgagca
      121 ggccgagacc accggcatac ccggcgaaga ccggccgggg gtcggtggcg gaggcacgag
      181 ccagctcggc cgccgtcgcg accagcccgt gccctgaggc cgcagcggcg tagaaggcgg
      241 gcaggcttgc tgcggccgag atccggcggg cgctcccggc atagaccccg gccaggagcg
      301 gctcgacgag ccggtcgagc acggcggtcc ccagccggcc ggccacgagg tcccccaccg
      361 agatgtccga gctcactggt tccgggtggg ccggctcggc gtagatgcga gccacctcgg
      421 catcggtgag cagcccccgc gtcgtctccg ggtccgacgg gatccccatg agagtccgcg
      481 gcggcagcgg ccagagcctg ccccgtgagt agaccgaggc tgccgtgacg tccggatggg
      541 tcaggccccc cgcgagcccg gcctcctcga cgaggccgac cgcctcagga cggcgggcga
      601 gcatcgactc ggcgccgatg tcgattcggt gcccggccac gacgcccgag gcgagcttgc
      661 cgcccgggac cggtgcggcg tcatacacgc agatggacag cgcgcgacct gcgcgggtga
      721 gctgccaggc ggcggcgaga ccgctgaccc cgccaccgat cacgacgaca tcgaagggcg
      781 ccacggcgcc caccctcgca catccgtcga cgcgctcggc aaccacctgc ggcgcgaccg
      841 tgacccgatc gagactggag cgaaggaacc ccggtcgccg acgagacctc ccaaggtgac
      901 agaccgacgc cggcgcagct cctggaggtt gacgtgatga cgaaccggat ccccctcgcc
      961 ctcggggctg gcttggccac ggtgatgctc gcccttgtcg ggtgctcagg tgactcggca
     1021 accacgtcgg gcgccgctcc cggtggcgcc gaggcggccc ccgcccccgg agcaccggct
     1081 cgggacggtg ccgtcgccgg tggtgacagc aaggcaggga cgaccttggt cacggcccag
     1141 aaggtcatcc gcaacgcgac ggtcctgctc gaggttcccg acgtggccaa ggccgccgcg
     1201 accgtgcgca cgattgccgc gggcgcccag ggcgtggtga cgtccgagca gctgagcagc
     1261 ggaagcgacg tgccgaagcc ccagcccgcc gaccaggtcg ctcccacccg cttcgcggac
     1321 gccacgatcg tcatctcggt gccgcagagc tcactggacc gagtcctcga caggctctcg
     1381 gccatcggca cgctgacgag ccgtcagctc acctcccagg atgtcaccgc gacgtttgtc
     1441 gacacgacgt cacgggtcgc gtcgatgaag gccagcgtcg agcgcatccg cgccctcatg
     1501 gccaaggcga cgagcatcac cgacctcgcc tcgctcgagg gcgagctgag ccggcgccag
     1561 accgacctcg aggcgatgca gtcccagctc aaggcactcg aggaccaggt ggcgatgtcg
     1621 tcggtcacgg tgaccatggc ggggcctggg acgacgctgc gtgaggacac ggactggggc
     1681 ttcttcaacg gcctcaggaa cggtctggaa g
//
LOCUS       DATAIX010000099         2370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_35539, whole genome shotgun sequence.
ACCESSION   DATAIX010000099 DATAIX010000000
VERSION     DATAIX010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35539"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VHM65_01550"
     CDS             complement(<1..341)
                     /locus_tag="VHM65_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704415.1"
                     /translation="MWLAEDSPAEESIGDRDLTEWRSVTGRAMARAVSRLAGRVATAA
                     RANLVLLTTPGAGILLAGGLAALAAGVYDAVTEGDGIAVLDRPALNAAESLRSPGLNH
                     AVTWFTSLGGTV"
     gene            548..997
                     /locus_tag="VHM65_01555"
     CDS             548..997
                     /locus_tag="VHM65_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX2704416.1"
                     /translation="MTALDSDECWAVLRSQEFGRMAYHDGESVSLVPINYAVDEQRRL
                     VFRTAEGEKLLAVKDNDDIAFEVDEITESTATSIILRGRAHELTGAEAAWVDTTRLRP
                     WVPTSKVHVIAIDPFEVSGRTFQLARPWLHMVPDHPIAPRTRRSRAS"
     gene            1279..2178
                     /locus_tag="VHM65_01560"
     CDS             1279..2178
                     /locus_tag="VHM65_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704417.1"
                     /translation="MTASTTASTTAPPGGSVTTAAAPGPPASSTRAGALLDLPRGGRT
                     IFPAYRLVGFSGAPGSAALGRLGIGAIDDRVSEIERLGASYAKGRTVLPVLELIATVA
                     HRSPGRDDMFRSRASDATISRYLAAARRHRGILLLNISPGRARFLDEVTAYARWLAEP
                     DVGLALDPEWAVGPGQVPGRVFGHTSGAELDSIARYVAGLVAAKRLPEKVVLYHQLHV
                     SIVAGESALTPHPGVVLVKSVDGIGTPAQKASTWRSILRRTPTYVHPGFKLFFIEDRE
                     HGPLMTPAQVLALRPTPEYVLYE"
     gene            complement(2169..>2370)
                     /locus_tag="VHM65_01565"
     CDS             complement(2169..>2370)
                     /locus_tag="VHM65_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704418.1"
                     /translation="GFERGDSEHHNLTRRGRRAGTRVRPCYAAWSGRSPPQRVLPTAA
                     QAAGELPSYTLQSLGARAGPHS"
BASE COUNT          326 a          876 c          836 g          332 t
ORIGIN      
        1 accgtgccgc ccaggctggt gaaccacgtc acagcgtggt tcaggccggg gctgcgcagg
       61 gactcggccg cgttgagcgc cggccggtcg aggaccgcga tcccgtcacc ctcggtcacg
      121 gcgtcgtaga cacccgcggc cagggccgcg agcccgccag ccagcaggat cccggctccc
      181 ggcgtggtga gcaggacgag gttggcccgg gcggctgtcg ccacccgacc ggccagccgc
      241 gacacggctc gcgccatggc ccgtccggtg acgctgcgcc attcggtcag gtcacgatcg
      301 ccgatacttt cttcggcggg cgagtcctca gccagccaca tgtcgctcat tgtccgccgt
      361 ccgcacggct tcgtggctac ggaccgcccg gagctggcct cacccccagc acggaatcgt
      421 ggcgtaggcg acactcgaca acacccggat cgggcgcgtt ggcagggaac tgcggcatac
      481 cctggaattg gcgacccgag tgaggtggcc gaacgggggt acggcgatgg aacacgaccc
      541 gcgcgtcgtg accgcactcg attcggacga gtgctgggcc gtcctgcgct cccaggagtt
      601 cggccggatg gcctaccacg acggggagtc ggtgtccctc gtgcccatca actacgccgt
      661 cgacgaacag cgcaggctgg tcttccgcac ggccgagggc gagaagctgc tcgccgtcaa
      721 ggacaacgac gacattgcct tcgaggtcga cgagatcacc gagtcgacgg cgacgagcat
      781 catcctgcga gggcgcgcgc acgagctcac cggggccgag gccgcctggg tcgacacgac
      841 ccggctgcgg ccgtgggtgc cgacaagcaa ggtccacgtc atcgccatcg acccgtttga
      901 ggtgtccggg cggaccttcc agctcgcccg gccctggctc cacatggtgc ccgaccatcc
      961 gattgcaccc cgcacgcgca gatcccgcgc ttcctgaacg ggagtcggtc gcactgtggc
     1021 tccgtagccc cccggccgcg ggccgggggg ctacggcacg agacggtagc cgatgccggg
     1081 ctcggtgtgg cgacctcccc atgaccctgc ctcgctaggc tcgtccaagt gcggctctcc
     1141 cccggcaccg gacgctcgga ccgggcaacc cggcaccgac ggcaccaccg cagcgccgct
     1201 gccgtggtgc aggtgctcgc cgcggccgct gcctgcacga cctctgcctg caccggcccg
     1261 ccggtcaccg gaccgaccat gactgcctcc acgactgcct ccacgacggc cccgccgggt
     1321 ggctcggtca cgactgcggc ggctcccggg cccccggcca gcagcacccg tgccggtgcg
     1381 ctgctcgacc tgccgcgcgg cgggcggacg atcttcccgg cataccggct cgtggggttc
     1441 tccggcgcgc cagggtcggc tgccctggga cgtctcggga tcggcgcgat cgacgaccgg
     1501 gtgagcgaga tcgagaggct cggcgcgtca tacgcgaagg gccgcacggt cctgccggtc
     1561 ctggagctca tcgccacggt cgcgcaccgg tcgcccggtc gcgacgacat gttccggtcg
     1621 cgggccagcg atgcgacgat ctcgcgctac ctcgcggcgg cgaggcggca ccgggggatc
     1681 ctgctgctca acatctcgcc gggccgggcg aggttcctcg acgaggtcac ggcgtacgcc
     1741 cgctggctcg ccgagcccga tgtcgggctc gcgctcgacc cggagtgggc cgtcgggccg
     1801 gggcaggtgc cgggccgggt gttcgggcac acctccggcg ccgagctcga cagcatcgcc
     1861 cggtatgtcg cgggcctcgt cgccgcgaag cgcctgcccg agaaggtcgt gctctaccac
     1921 cagctgcacg tctcgatcgt cgccggcgag tcggcgctga cgccccaccc gggggtggtg
     1981 ctggtcaagt cggtcgacgg catcggcact ccggcgcaga aggcatcgac gtggcggtcg
     2041 atcctcaggc ggaccccgac gtacgtccac ccgggcttca agctcttctt catcgaggac
     2101 cgcgagcacg gcccgctcat gacgcccgcc caggtcctcg cgctcaggcc gacgccggag
     2161 tacgtcctct acgagtgagg cccggcccgg gcaccgaggg actggagggt gtatgacggg
     2221 agctcgcccg cggcctgcgc ggcggtggga agcacccgct ggggtggcga cctgccgctc
     2281 catgccgcgt agcatggccg gactcgggtg cctgcccgac gtcctcgacg ggtgaggttg
     2341 tgatgctcgg agtcgccccg ctcgaagccc
//
LOCUS       DATAIX010000100         1695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_270735, whole genome shotgun sequence.
ACCESSION   DATAIX010000100 DATAIX010000000
VERSION     DATAIX010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270735"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VHM65_01570"
     CDS             <1..854
                     /locus_tag="VHM65_01570"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX2704419.1"
                     /translation="SSVGGRFLMAFRDNLTHPGFLLPPLPNTLFTRDNTSWIFGGVTL
                     NPMYWPARRQETLLTTAIYHFHPEFASLDFPVWFGNPDTDHGMATLEGGDVMPIGKGV
                     VVIGMGERTSQQAVTQVARALFQAGAAERVIVAALPKTRAAMHLDTVFTFCDRDVVTT
                     YDTVTDAIVPLSLRPDDSIPSGLDIRCEESSFVDTVGEALGVRLRVVPTGGDRYGAAR
                     EQWDDGNNVVALEPGVVVGYDRNTHTNTRLRKAGIEVITITAGELGRGRGGGRCMTCP
                     ILRDGVD"
     gene            967..>1695
                     /gene="argF"
                     /locus_tag="VHM65_01575"
     CDS             967..>1695
                     /gene="argF"
                     /locus_tag="VHM65_01575"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX2704420.1"
                     /translation="MAYNNHNRNVLSLVHHTERDLSHLLSLARDLKRAKYSGVEQQRL
                     RGKNIALIFEKTSTRTRCAFEVAAYDQGANVTYIDPSSSQIGHKESMKDTARVLGRMY
                     DAIEYRGFAQESVEILAANSGVPVYNGLTDEFHPTQMLADVLTMLEYCPKPIYDIAFA
                     FVGDGRNNVANSLLLVGAKLGMDVRIGAPRALWPSEEHVALCQGFARDSGARLTVTDD
                     VQAAVAGVDFIHTDVWVSMGEPIET"
BASE COUNT          278 a          625 c          545 g          247 t
ORIGIN      
        1 cctcgagcgt cggcgggcgc ttcctcatgg ccttccggga caacctcacg cacccggggt
       61 tcctcctgcc gccgctgccc aacaccttgt tcacccgcga caacacgtcg tggatcttcg
      121 gcggggtgac cctcaacccg atgtactggc ccgcccgccg ccaggagacg ctgctcacga
      181 cggcgatcta ccacttccac cccgagttcg cctccctcga cttcccggtg tggttcggca
      241 acccggacac cgaccacggg atggccaccc tcgagggcgg ggacgtcatg ccgatcggca
      301 agggtgtcgt cgtgatcggg atgggggagc gcacctcgca gcaggccgtc acccaggtgg
      361 cccgggcgct gttccaggcc ggggccgccg aacgcgtcat cgtggcggcg ctgcccaaga
      421 cccgggccgc gatgcacctc gacaccgtgt tcacgttctg cgaccgcgac gtcgtgacga
      481 cctacgacac cgtgaccgac gcgatcgtcc ccctcagcct gcgtccggac gactcgatcc
      541 cctcgggtct ggacatccgc tgcgaggagt cctcgttcgt cgacacggtc ggcgaggccc
      601 tcggcgtccg gctccgggtg gtccccaccg gcggggaccg ctacggcgcc gcccgcgagc
      661 agtgggacga cgggaacaac gtcgtcgcgc tggagcccgg cgtcgtggtc ggctacgacc
      721 gcaacaccca cacgaacacc cgcctgcgta aggccgggat cgaggtcatc accatcaccg
      781 cgggtgagct cggccgcggg cgcggaggcg ggcgctgtat gacgtgcccg atccttcgcg
      841 acggggtcga ctgacgcccg agccggctcg tgaccgaggt cacgcgctcg ccgagccggg
      901 acgcgtcata cccgactcgt cccccagccc acccccgact gccagcccat ccgaaggaga
      961 aggtccgtgg cttacaacaa ccacaaccgc aacgtcctga gcctggtcca ccacaccgag
     1021 cgagacctga gccacctgct cagcctggct cgcgacctca aacgggcgaa gtactccggc
     1081 gtcgagcagc agcgcctccg gggcaagaac atcgcgctga tcttcgagaa gacctcgacc
     1141 cggacgcggt gcgccttcga ggtcgcggcc tacgaccagg gcgccaacgt cacctacatc
     1201 gacccgagct cctcgcagat cgggcacaag gagtcgatga aggacacggc tcgggtcctc
     1261 gggcggatgt atgacgcgat cgagtaccgc ggtttcgccc aggagtcggt cgagatcctc
     1321 gccgccaact cgggcgtgcc ggtctacaac gggctgaccg acgagttcca tcccacccag
     1381 atgctcgccg acgtcctgac gatgctcgag tactgcccca agccgatata cgacattgcc
     1441 ttcgccttcg tcggcgatgg tcgcaacaac gtcgcgaact ccctgctgct cgtcggggcc
     1501 aagctcggga tggacgtgcg catcggcgca ccccgcgcgc tgtggccgag cgaggagcac
     1561 gtcgcgctgt gccagggctt cgcgcgggac agcggcgccc ggctgacggt caccgacgac
     1621 gtccaggcgg ccgtggcggg tgtcgacttc atccacaccg acgtgtgggt gtccatgggc
     1681 gaaccgatcg agacc
//
LOCUS       DATAIX010000101         1900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_154866, whole genome shotgun sequence.
ACCESSION   DATAIX010000101 DATAIX010000000
VERSION     DATAIX010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154866"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1330)
                     /locus_tag="VHM65_01580"
     CDS             complement(179..1330)
                     /locus_tag="VHM65_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2704421.1"
                     /translation="MKRLTEEEQSVVDLVHEFVENEVRPVARDLEHANTYPEALIEAM
                     KRMGIFGLAIPEPYGDVQVSTACYVMVTEELSRGWMSLAGAMGGHTVVSKLLLLFGTE
                     EQKQQYLPRMATGELRATMALTEPGGGSDLQAMRTVARRDGDHYLINGSKTWITNARR
                     SGLVALLCKTDPAAQPVHTGMSILLVEKVPGFVVHADLPKLGYKGVETCELSFDNAQV
                     PGTAVLGGVEGKGFAQMMKGLEVGRLQVAARATGVGRAAFDDALSYAQERHSFGKPIW
                     RHQSVGNYLADMATKLSAARQLLLYAADRLDSGERCDMEAGMAKLFASETAMEIALNA
                     MRVHGGYGYSTEFDIERYYRDAPLMIVGEGTNEIQRNVIVSQLVARGGL"
     gene            complement(1327..>1900)
                     /locus_tag="VHM65_01585"
     CDS             complement(1327..>1900)
                     /locus_tag="VHM65_01585"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX2704422.1"
                     /translation="DLEDAVAAGEKSAARSALVDWVHAGGRPMVRVNACGTPWYDADV
                     AVCAEAGVPVLLPKSDDPGALADLVTRLGAGARVIPLVETARGVEHALALACEPGVVR
                     LALGALDLAAEVGFHPADREALLYTRSRLASASAAAGLAGPVDGVTTGVADAAGAVVI
                     LDGRMVDRPVVARAQRLLASIDPSTGGGAS"
BASE COUNT          272 a          700 c          648 g          280 t
ORIGIN      
        1 gtccctggtc accgccgccc ggccggcctc gcccgggcgg cggtgaccag ggactgggcg
       61 ctcatccact gcgcgaacgc gaacgtgcca cgaggctggc ccggcccgcg cgcgctccca
      121 cgagggtctt gcagcagcgg gcgcaccggt tcgccccttg gcgtcgcgtg accactggtc
      181 acagcccacc cctggcgacg agctggctga cgatgacatt gcgctggatc tcgttcgtgc
      241 cctcgccgac gatcatgagc ggcgcgtcgc ggtagtaccg ctcgatgtcg aactcggtcg
      301 agtagccgta tccgccgtga actcgcatgg cgttgagggc gatctccatc gcggtctccg
      361 aggcgaacag cttggccatc ccggcctcca tgtcgcaccg ctcccccgag tcgagccggt
      421 ctgcggcgta caagagcagc tggcgcgcgg ccgagagctt ggtggccatg tcggcgaggt
      481 agttgccgac cgactggtgc cgccagatcg gcttgccgaa gctgtgccgc tcctgggcat
      541 agctcaacgc gtcgtcgaag gcggcgcggc cgacccccgt cgcccgcgct gcgacctgca
      601 gccggccgac ctccagaccc ttcatcatct gcgcgaaacc cttgccctcg acgccgccga
      661 ggaccgccgt cccggggacc tgcgcgttgt cgaaggacag ctcgcaggtc tcgactccct
      721 tgtagccgag cttgggcagg tcggcgtgga cgacgaagcc cgggaccttc tcgaccagca
      781 ggatgctcat tcctgtatgc acgggctgcg ccgccgggtc ggtcttgcac agcagcgcga
      841 cgaggcccga ccggcgcgcg ttggtgatcc aggtcttgga tccgttgatg aggtagtggt
      901 cgccgtcgcg ccgggcgacc gtgcgcatcg cctgcaggtc cgagccgcca cccggctcgg
      961 tcagcgccat cgttgcgcgc agctcaccgg tcgccatccg gggaaggtac tgctgcttct
     1021 gctcctcggt gccgaagagc aggagcagct tggagaccac cgtgtgacca cccatcgccc
     1081 cggccaggct catccacccc cgggacagct cctcggtcac catgacgtag caggcggtgg
     1141 acacctggac gtcgccatac ggctcgggga tggccagccc gaagatcccc atccgcttca
     1201 tcgcctcgat gagcgcctcg gggtaggtgt tggcgtgctc gaggtcccgg gcaacgggac
     1261 gcacctcgtt ctcgacgaac tcgtgcacga ggtccaccac cgactgctcc tcctcggtta
     1321 gccgcttcat gaggcccctc ctcctgtgct cgggtcgatg ctggcgagca ggcgctgggc
     1381 gcgggccacg acgggccggt ccaccattcg cccgtcgagg atcacgacgg ctccggccgc
     1441 gtcggcgacg ccggtcgtga ccccgtcgac gggaccggcg agaccggccg ccgcgctcgc
     1501 cgaggccagc cgggaccggg tgtagagcag tgcctcgcgg tcggccgggt gaaagcccac
     1561 ctcggccgcg aggtcaaggg ccccgagggc aaggcggacg acacctggct cgcaggccag
     1621 cgccagcgca tgctcgaccc cccgggcagt ctccaccaga ggtatgacgc gcgccccggc
     1681 tcccagccgg gtcaccaggt cggcaagagc cccagggtcg tcggacttgg gcagcagcac
     1741 cgggacgccg gcctcggcgc agaccgccac gtccgcgtca taccagggcg tcccgcaggc
     1801 gttgacgcgg accatggggc ggcctcccgc gtgaacccag tccaccaggg ccgaccgggc
     1861 agcggacttc tcgccggcag ccacggcgtc ctcgaggtcg
//
LOCUS       DATAIX010000102         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_211095, whole genome shotgun sequence.
ACCESSION   DATAIX010000102 DATAIX010000000
VERSION     DATAIX010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211095"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VHM65_01590"
     CDS             complement(<1..818)
                     /locus_tag="VHM65_01590"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX2704423.1"
                     /translation="MKGILAGAVISLVCSLFGTPLLIAFLVRRGYGQFIRDDGPTSHH
                     TKRGTPTMGGAVIILSSLLAYGGAHLFTLSRPTASAMLVIFLMAGLGVVGFLDDYIKI
                     SRQRSLGLRSREKLAGQGLVGVIFAVLALQFPNDRFRTPAAPFISFVRDTEVNLAFAG
                     TALGVVLFVIWANLMIAGFANGVNLTDGLDGLATGASTMVFAAYVLIGIWTSNQNCQI
                     NAGTKCYEVRDSLDLAVLAACAMGASFGFLWWNASPAKIFMGDTGSLALGGVLSG"
     gene            complement(847..>2083)
                     /gene="murF"
                     /locus_tag="VHM65_01595"
     CDS             complement(847..>2083)
                     /gene="murF"
                     /locus_tag="VHM65_01595"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX2704424.1"
                     /translation="RYAGPARSGGAVAALTTRLVSELPCVVVADVQTAFGRVARAVID
                     RSPGLTVIAITGSSGKTTTKDLLAHVLSVAGVTVAPVDSLNGEIGVPLTVCRVTLVTR
                     FLVAEMGARGIGHLTYLTGIAPPHIGVVLNVGRAHAGEFGSRENTARAKAELVAALPP
                     QGLAVLNGDDPLVRAMARQSAARVVLTGEAADADVRAVDVVVDDSGRATFTAVTPAGS
                     RPIHLRLVGRHHVGNALAVLAVALECGLRLDLAGEAIEGARPVSRWRMEVIERPDGVT
                     VVNDAYNANPDSMAAALTALAGLRREGTPGRRWAVLGQMLELGAESDAAHAEAGALAA
                     SLGIERVVAVGPGARAIGAGATQSGTGTRVSFVADSDAAYRLLSEQLRDGDVVLLKSS
                     RDAGLRWLGDRLVGLEVPS"
BASE COUNT          347 a          711 c          766 g          259 t
ORIGIN      
        1 cctgagagca ctcccccgag agcaagcgag cccgtgtcgc ccatgaagat cttggcgggc
       61 gaggcgttcc accagaggaa cccgaaactg gcgcccatcg cacacgcggc aagcacggcg
      121 aggtcgaggg agtcgcgcac ctcgtagcac ttcgttccgg cgttgatctg gcagttctgg
      181 ttggaggtcc agatcccgat gaggacgtag gcggcgaaga ccattgtcga cgcgccggtg
      241 gcaaggccgt cgaggccgtc ggtgaggttc acgccgttgg cgaacccggc gatcatgagg
      301 ttggcccaga tgacgaacag gacgacgccg agcgcagttc cggcgaaggc caggttgacc
      361 tcggtgtcac gcacgaacga gatgaacggt gcggcggggg tccggaagcg gtcgttgggg
      421 aactgcaggg ccaggaccgc gaagatcacc ccgacgaggc cctggccggc gagcttctcc
      481 ctcgagcgca gaccgaggct gcgctggcgc gagatcttga tgtagtcgtc gaggaagccg
      541 acgaccccga gcccggccat caggaagatg acgagcatgg ccgaggccgt cggccgggac
      601 aaggtgaaca ggtgcgcgcc gccatacgcg agcagcgagg acaagatgat gacggccccg
      661 cccatggtgg gagtgccgcg tttggtgtgg tgggaggtcg gtccgtcgtc gcggatgaac
      721 tggccgtacc cacggcgaac gaggaaggcg atgagcagcg gcgtgccgaa cagcgagcag
      781 acgagcgaga tcaccgcccc ggcgagaatg cctttcacgg gcgcctgccc tggccgcacc
      841 tgcggttcac gaggggacct ccagcccgac gagtcgatct cccagccacc gtagccctgc
      901 atcgcggctc gacttgagca acacgacgtc accatcgcgc agttgttcgg acaggagccg
      961 gtatgccgcg tccgagtccg cgacgaagct cactctggtc ccggtgcccg actgggtcgc
     1021 gccggcaccg atcgcgcggg caccggggcc gacggcgacg acgcgctcga tgcccagcga
     1081 cgcggcgagc gccccggcct cggcgtgggc cgcatcggac tcggcgccga gctcgagcat
     1141 ctgcccgaga accgcccatc tccggccagg cgtgccctcg cggcgcaggc cggccagggc
     1201 ggtcagggcg gccgccatcg agtcggggtt ggcgttgtag gcgtcgttga cgacggtcac
     1261 cccgtcggga cgttcaatga cctccatgcg ccagcgactc accggccggg ccccctcgat
     1321 ggcctcaccg gcgaggtcga ggcgcaggcc acactcgagg gcgaccgcga ggacggcgag
     1381 cgcattgccg acatggtgcc gtccgacgag acgcaggtgg atcggccggg acccggcggg
     1441 cgtcacggcc gtgaaggtgg cccggcccga gtcgtcaacg accacatcga ccgcccgcac
     1501 gtcggcgtcc gcggcctcac cggtgagaac gacccgggcc gcgctctgcc gcgccatggc
     1561 gcggacgagc gggtcgtcgc cgttgaggac ggccagcccc tgcggcggca gggcggcgac
     1621 gagctcggcc ttggcccggg cggtgttctc gcgcgagccg aactcaccag catgagcgcg
     1681 gccgacgttg aggacgacac cgatgtgcgg cggggcgatc ccggtgaggt aggtcaggtg
     1741 cccgatcccg cgggcaccca tctcggcgac gaggaagcgg gtcaccaggg tgacccggca
     1801 cacggtcagc ggcacgccga tctcgccgtt gagcgagtcc accggggcca ccgtcacccc
     1861 cgcgacggac agcacgtggg ccagcaggtc cttcgtcgtc gtcttgcccg aggacccggt
     1921 gatggcgatg acggtgagcc cggggctgcg gtcgatgacg gcacgggcga cccgcccgaa
     1981 ggcggtctgg acgtccgcga cgacgacaca gggcagctcg ctgacgagcc gcgtcgtcag
     2041 cgccgccacg gcgccccccg accgcgccgg cccggcatac cga
//
LOCUS       DATAIX010000103         2331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_95312, whole genome shotgun sequence.
ACCESSION   DATAIX010000103 DATAIX010000000
VERSION     DATAIX010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95312"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VHM65_01600"
     CDS             complement(<1..498)
                     /locus_tag="VHM65_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX2704425.1"
                     /translation="MLTYDDDLRLAHVLADAAERITMTRFKAEDLRVESKPDLTLVSD
                     ADRATEELIRAQLGRTRPRDSVVGEELETTGHGQRRWIVDPIDGTHSFVRGVPVWATL
                     IALVDGDDPVVGLVAAPALSRRWWAAKGSGTWSGRSLSAARQIRVSQVSSLSDASISY
                     ASLHSW"
     gene            complement(534..1580)
                     /gene="rsgA"
                     /locus_tag="VHM65_01605"
     CDS             complement(534..1580)
                     /gene="rsgA"
                     /locus_tag="VHM65_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX2704426.1"
                     /translation="MSWRDLDESDVRARPNRRGSRPRSKDRPLHEDAIAGVVTGVDRG
                     RYAVLVAAGVGGRASAERTITAMRARELGRKGIVVGDEVDMVGDTAGGADALARIIRL
                     HPRRSVLRRTADDTDPVERVIVANADQLVIVSALASPEPRPRLIDRCLVAAYDAGLTP
                     LLVLTKADLVSPQDFLATYAPLDVAAVVTSRPDVKSDGPSRALDDLRARLSGRVSVFV
                     GHSGVGKSTLVNALVPGADRATGVVNDATGRGRHTSSSAVALRLPGRDGWVIDTPGLR
                     SFGLAHVDLARIISHFPDLDAGTAECPRGCTHDEAECGLDDWAASGSAGPNGPARLES
                     LRRLLRARSSSAED"
     gene            complement(1577..>2331)
                     /locus_tag="VHM65_01610"
     CDS             complement(1577..>2331)
                     /locus_tag="VHM65_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704427.1"
                     /translation="LAGARYDEGVTVRHRGRPVPSEPHIAMTVETLRDVGVIVDDDDA
                     NLWRVEPSEINALDVKVEPDLSSAGPFVAAALVAGGTVAIPGWPRHTTQGGDALRQLL
                     SDMGADVRLSRSGLTVSGTGTIAGIDIDLHDASELTPVVAALAALADTPSRIRGVAHI
                     RGHETDRITALATEINRLGGQVSETEDGLRIQPARLKGKRFRTYHDHRMAMAGAVLGL
                     RVRHLVVEDIATTAKTLPGFTELWMAMLDGTR"
BASE COUNT          322 a          802 c          889 g          318 t
ORIGIN      
        1 ccacgagtgc aaggaagcgt aggagatcga cgcgtccgag agcgacgaga cctgtgacac
       61 gcgaatttgc ctggcagcag acaggcttcg cccgctccag gtcccagagc ccttggctgc
      121 ccaccacctc ctcgacagcg ctggcgcggc gacgagaccg acgaccgggt cgtcgccgtc
      181 gacgagcgcg ataagggtgg cccagaccgg gacaccgcgg acgaagctgt gcgtgccgtc
      241 gatcgggtcg acgatccacc gccgctggcc gtggcccgtc gtctcgagct cctcgccgac
      301 caccgaatcg cggggccggg tccggccgag ctgggcccgg atcagctcct cggtggcgcg
      361 gtcggcatcg ctgacgaggg tgaggtcggg cttggactcg acgcgcaggt cctcggcctt
      421 gaagcgcgtc atcgtgatcc gctccgcggc atcggccagc acatgggcaa ggcgcaggtc
      481 gtcgtcatac gtcagcaccc gcccaaccta gtctgtgggc gcgggggccg cgatcagtcc
      541 tccgcggagg aggagcgggc ccgcagcagc cggcgcagcg actccaggcg tgcggggccg
      601 ttcggcccgg ccgaacccga ggcagcccag tcgtcgagcc cgcactcggc ctcgtcatgg
      661 gtgcagccgc gtgggcactc ggccgtcccc gcgtcgaggt ccgggaagtg ggagatgatc
      721 cgggccaggt cgacgtgggc gagcccgaac gagcgcaagc ccggggtgtc gatcacccag
      781 ccgtcccgcc cggggaggcg cagtgcgacg gccgaggacg aggtatggcg cccacgtccg
      841 gtcgcgtcgt tgaccacccc ggtggcgcgg tcggcaccgg ggacgagggc gttgaccagg
      901 gtcgacttgc cgacgccgga atgcccgacg aagacgctga cccggccgga caggcgagct
      961 cgcaggtcgt ccagcgcgcg gctcggaccg tcgctcttca cgtcgggacg cgaggtgacg
     1021 acggcggcca cgtcgagtgg agcgtaggtg gccaggaagt cctggggcga gacgaggtcg
     1081 gccttggtca ggacgagcag cggcgtgagc ccggcgtcgt aggcggccac gaggcagcgg
     1141 tcgatgagcc gggggcgggg ctccgggctg gccagggccg agacgatgac gagctggtcg
     1201 gcgttggcca cgatgacccg ctcgaccggg tcggtgtcgt cggccgtgcg gcgcaggacc
     1261 gaccggcgcg ggtgcagccg gatgatacgc gccagcgcgt cggcgccacc ggcggtgtcg
     1321 ccgaccatgt cgacctcgtc gccgacgacg atgcccttgc ggccgagctc acgggccctc
     1381 atcgcggtga tcgtccgctc ggcactggct cgcccaccga cgccggcggc gacgagcacg
     1441 gcatagcggc cccggtcgac cccggtgacg actccggcga tggcgtcctc gtgcaacggt
     1501 cggtccttgg aacgcgggcg gctgccccgg cggttggggc gggcccggac gtcggactcg
     1561 tcgaggtcgc gccagctcac cgggtgccgt ccagcatggc catccagagc tcggtgaagc
     1621 cgggcagggt cttggcggtg gtggcgatgt cctcgacgac gaggtggcgc acccgcagtc
     1681 cgagcaccgc cccggccatg gccatccggt ggtcgtggta ggttcgaaaa cgctttccct
     1741 tgaggcgagc cggctggatc cggagcccgt cttcggtctc gctgacctgg ccaccgagcc
     1801 ggttgatctc ggtcgcgagc gccgtgatgc ggtcggtctc gtggccccgg atgtgggcga
     1861 ccccgcggat ccgcgacggg gtgtccgcga gggccgcgag cgccgccacg acgggggtca
     1921 gctcgctcgc gtcgtgcagg tcgatgtcga tcccggcgat ggtgccggtg ccgctgacgg
     1981 tgagcccgga ccgggacagc cgcacgtcgg cgcccatgtc cgagaggagc tggcgcagcg
     2041 catcgccgcc ctgagtggta tggcgtggcc atccggggat ggccacggtg cctcccgcga
     2101 cgagcgccgc cgcgacgaac gggccggcgc tggacaggtc gggctcgacc ttgacgtcca
     2161 gggcgttgat ctcgctcggc tcgacccgcc acaggttggc gtcgtcgtca tcgacgatga
     2221 cgccgacatc gcgcagggtc tccaccgtca tcgcgatgtg cggctcgctc ggcacgggtc
     2281 tgccacggtg gcggaccgtc accccctcgt cataccgggc tccggcgagg a
//
LOCUS       DATAIX010000104         1619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_211131, whole genome shotgun sequence.
ACCESSION   DATAIX010000104 DATAIX010000000
VERSION     DATAIX010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211131"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1393
                     /locus_tag="VHM65_01615"
     CDS             119..1393
                     /locus_tag="VHM65_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2704428.1"
                     /translation="MDFAPSPRAAGLTARVRAFVADEIAPREPAYDREITRRRHTGED
                     SWVPLPLVAELQAKARARGLWNLFLPAGHGDGYAARFGTDGGTGLTNLDYAPVAEQTG
                     RSFLAPLVLNCNAPDTGNMEVLLRYGTTAQQERWLEPLLDGRIRSAFLMTEPEVASSD
                     ATTMRTIGRIEGEEVVLSGRKWWSTGVGHPGCAIFIVMALTNPEAPRHAQHSMVLVPR
                     DAAGVRIERLLPALGQYDEPLGHGEVSLTDVRVPLANVIAGPGRAFEIAQGRLGPGRV
                     HHCMRLIGLAERCLELACRRGTDRVAFGRPLVDLGGNRERIAEARIAVNQARLLVHHA
                     AWKLDTEGPAGSLSEVSQIKVAVPAMAQRVVDMALQLHGGGGLSDDFPLAAALASARS
                     LRLADGPDEVHRELIARLELKKYRDPASLRHP"
     gene            1393..>1619
                     /locus_tag="VHM65_01620"
     CDS             1393..>1619
                     /locus_tag="VHM65_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704429.1"
                     /translation="MSQDVQPVRAEDSLDLDAVAGWLRSARPDLPALSGTPELRQFGG
                     GASNLTYLLGYPAAALVLRRPPAGTKAKSAH"
BASE COUNT          217 a          624 c          555 g          223 t
ORIGIN      
        1 atccacaggc ggcaccgtgg tactcgacaa gggggacctg ggctggcagc cgacgcgcag
       61 ccgtcgaccg gcgccggtcg tcggcgcgca gccgccgagc gggagtacgg tcgggtgtat
      121 ggactttgcc ccatcgccgc gggcggctgg cctcacggct cgggtccgcg ccttcgtcgc
      181 ggacgagatt gccccccggg agcccgccta cgaccgcgag atcacccgtc ggcgccatac
      241 gggagaagac tcatgggtgc cgctgccgct tgtcgcagag ctccaggcca aggcgcgggc
      301 ccgcggtctg tggaacctct tcctgccggc cgggcacggt gacggctacg cggcgcgctt
      361 cggcaccgac gggggcaccg gcctgaccaa cctcgactac gcccccgtcg ccgagcagac
      421 cggccggtcc tttctcgccc cgctcgtcct caactgcaac gcccccgaca caggcaacat
      481 ggaggtcctg ctgcgctacg gcaccacggc ccagcaggag cggtggctcg agccgctgct
      541 cgacggccgc atccgcagcg ccttcctcat gaccgagcca gaggtggctt cctccgacgc
      601 gacgacgatg cggacgatcg gacggatcga gggcgaggag gtggtcctgt ccgggcgcaa
      661 gtggtggtcc accggcgtgg gtcaccctgg ctgcgcgatc ttcatcgtca tggcgctgac
      721 caacccggag gcgcccaggc acgcccagca ctcgatggtc ctcgtcccgc gcgatgccgc
      781 gggggtcagg atcgagcgcc tgctcccggc cctcgggcag tatgacgagc cgctgggcca
      841 cggggaggtg agcctgaccg acgtgcgagt ccccctggcc aacgtcatcg ccggacccgg
      901 gcgcgccttc gagatcgccc aagggcggct cggccccggg cgggtccatc actgcatgcg
      961 cctcatcggc ctcgccgagc gctgcctcga gctggcctgt cgccggggca ccgaccgggt
     1021 cgccttcggc cgtcccctcg tcgacctcgg cggcaaccgc gagcgcatcg ccgaggctcg
     1081 gatcgcggtc aaccaggccc gcctgctcgt ccaccacgcc gcgtggaagc tcgacaccga
     1141 gggtcccgca ggctcgctgt ccgaggtgag ccagatcaag gtcgccgtcc cggccatggc
     1201 acagcgggtc gtcgacatgg ccctgcagct gcacggcggc gggggtctgt cggacgactt
     1261 cccgctcgcc gcggccctgg ccagcgcccg ctcgctccgc ctggctgacg ggccggacga
     1321 ggtgcaccga gagctcatcg cccggctcga gctcaagaag taccgcgacc ctgcgtcgct
     1381 gcggcacccg tgatgtccca ggacgtgcag ccagtgcgcg ccgaggactc gctcgacctc
     1441 gacgcggtcg ccggctggct acgctccgct cgcccggacc tccccgcctt gtccggcact
     1501 cccgagctgc gccagttcgg cggcggggcg tccaacctca cctacctgct cggttatccg
     1561 gccgcggccc tcgtccttcg tcgcccgccg gccgggacca aggccaagag cgcccacga
//
LOCUS       DATAIX010000105         1765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_211135, whole genome shotgun sequence.
ACCESSION   DATAIX010000105 DATAIX010000000
VERSION     DATAIX010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211135"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1193
                     /locus_tag="VHM65_01625"
     CDS             138..1193
                     /locus_tag="VHM65_01625"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX2704430.1"
                     /translation="MPVSGTFGVPALNPDAGKQTKKEFTSDQEVRWCPGCGDYSILAA
                     VQSFMPELGLKRENIVFVSGIGCSSRFPYYMDTYGMHSIHGRAPAIATGIAVARRDLS
                     VWVVTGDGDALSIGGNHLIHALRRNINLKILLFNNEIYGLTKGQYSPTSPKGQVTKSS
                     PSGSVDTPFNPVSLALGAEATFVARAMDTDRAGLTEVLRAAAEHRGSAFVEIYQNCPI
                     FNDGIFDPIKDRTQAATRILHLREGEEVTAGDNVVVRAEGGALEVVPRLQATPADILI
                     HDSTREHPSVAFALSRLDDASFSHVPMGVFRNVARPTYDDLVRGQGDDAVAAAGGPPS
                     AADFNALFRGADSWTVG"
     gene            1281..>1765
                     /locus_tag="VHM65_01630"
     CDS             1281..>1765
                     /locus_tag="VHM65_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX2704431.1"
                     /translation="MSAYVIWGSFPLYFRALLPAGPFEILAHRIVWSLLVCALLLLVR
                     RDWRWVRPVLRRRRLMLGIALAAALIATNWVVYVAAVNTGHVTDASLGYFLNPIVTVA
                     LGVVVLHERPRPLQRLAVGIGFGAAVFLGVAAGTVPVIAMTLATTFGLYGLTKKRIGA
                     TL"
BASE COUNT          242 a          668 c          556 g          299 t
ORIGIN      
        1 ctgcctttcg cctcttccga gctcgccgcc gtgatcggcg ccgcgctcac cggccccccc
       61 tcgatgatcg gatcggcatg acactgcttg agcgcccgcc catccacgcc accgcgcatc
      121 ggcccgtcga ccttggtatg ccggtcagcg gcaccttcgg tgtcccggcc cttaaccccg
      181 acgccgggaa gcagacgaag aaggagttca cgtccgacca ggaggtacgg tggtgccccg
      241 gctgcgggga ctactcgatc ctggccgccg tccagtcctt catgcccgag ctcgggctca
      301 agcgggagaa tatcgtcttc gtctccggca tcggctgctc gtcgcggttc ccgtactaca
      361 tggacaccta cggcatgcac tcgatccacg gccgggcacc ggcgatcgcg accggcatcg
      421 ccgtggcccg tcgcgacctg tcggtgtggg tcgtcaccgg tgacggcgac gccctgtcca
      481 tcggcggcaa ccacctcatc cacgctctgc ggcgcaacat caacctcaag atcctgctct
      541 tcaacaacga gatctacggg ctgaccaagg gccagtactc ccccaccagc cccaagggcc
      601 aggtcaccaa gtcctcaccg tcggggtcgg tcgacacgcc gttcaacccg gtctcgctgg
      661 cgctcggcgc cgaggcgacc ttcgtcgccc gggcgatgga caccgaccgc gctgggctca
      721 ccgaggtcct gcgtgccgcc gcggagcacc ggggctcggc cttcgtcgag atctaccaga
      781 actgcccgat cttcaacgac ggcatcttcg acccgatcaa ggaccgcacc caggccgcga
      841 cgcggatcct tcacctgcgc gagggcgagg aggtcacggc cggcgacaac gtcgtcgtcc
      901 gggccgaagg gggcgccctg gaggtcgtcc cccgcttgca ggccacgccg gcggacatcc
      961 tgatccacga ctcgacccgc gagcacccgt cggtggcctt cgcgctgtcc cgcctggacg
     1021 acgcgtcgtt ctcgcacgtg cccatgggcg tcttccgcaa cgttgctcgt cccacctacg
     1081 acgacctcgt gcgtggccag ggcgacgacg ccgtggctgc ggccggtggc ccgccctcgg
     1141 cggccgactt caacgcgttg ttccggggag cggacagctg gaccgtgggc tgagtgggcc
     1201 gggaacgacc acggccactt atcgcctccc cgctcggggc atcgccttcg caggactccc
     1261 gtcagggggt cctctacggt gtgagtgcct atgtcatctg gggcagcttc ccgctctact
     1321 tccgcgcgct gctgcccgcc ggacccttcg agatcctggc ccaccggatc gtgtggtcgc
     1381 tgctcgtctg cgccctgctc ctgctcgtcc gacgagactg gcgctgggtc cgcccagtcc
     1441 tgcgtcggcg caggctcatg ctcgggattg cccttgccgc ggcgctcatc gcgacgaact
     1501 gggtcgtcta cgtcgccgcc gtgaacaccg gacacgtcac cgatgcctcg ctcgggtact
     1561 tcctcaaccc gatcgtcacg gtcgcgctcg gggtcgtcgt cctgcatgaa cggccccggc
     1621 cgttgcagcg gctcgcggtc ggaatcggct tcggggccgc ggtgttcctc ggcgtcgcgg
     1681 cgggcacggt ccccgtcatc gccatgaccc tcgcgacgac cttcgggctc tacgggctga
     1741 ccaagaagcg gatcggggcc accct
//
LOCUS       DATAIX010000106         3244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_155379, whole genome shotgun sequence.
ACCESSION   DATAIX010000106 DATAIX010000000
VERSION     DATAIX010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155379"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            465..1517
                     /locus_tag="VHM65_01635"
     CDS             465..1517
                     /locus_tag="VHM65_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX2704432.1"
                     /translation="MDVARFGTREARRVVQIRYGAAALDALDTTGLWAVVATFEGEVV
                     AAQLADVSPVEAPVADPAGWAPLDGSWSTTLDATAYRLAVQEVRGRVAAGAVYQSNIC
                     RVMTHPLSADADLGGLAALLRQGNPAPYASRVKVAEADLDIVCASPELFLSRRGTRLA
                     SSPVKGTAAPGAPMLDKDYAENVMITDLVRNDLSHVCLPGTVDVEVLCGAEDHPGLSH
                     LVTTVAGRLRPDATWAKIFAATFPPGSVSGAPKSSALRAIADLEPGPRGPYCGAVGWV
                     DADTREAELAVGIRTFWAEHDISGRRWLRFGTGAGITWGSDPQAEWEETELKARRLVA
                     LASGHGHGALTKGHRG"
     gene            1514..2278
                     /locus_tag="VHM65_01640"
     CDS             1514..2278
                     /locus_tag="VHM65_01640"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX2704433.1"
                     /translation="MTPEVRVWVNGGLVEAGSAAISALDHGVTVGDGVFETLKVVDGR
                     PFALSRHHRRLARSLAGLGLPGADFDHLAEGIEAVLAGEPIPFGRLRYTVTGGAGPLG
                     SDRHDCALTYIVTAGPSPRPADSAALSIVPWTRNERAATAGLKTTSYAENVMALAYAK
                     ERGALEAVFANTRGELCECTGSNIFVGTDGVLRTPPADSGLLPGVTRELVIEWCRQAG
                     LQVAEEALPIGVLADADEVFITSSTKDVLGVHAAGR"
     gene            2331..2822
                     /locus_tag="VHM65_01645"
     CDS             2331..2822
                     /locus_tag="VHM65_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02611 family protein"
                     /protein_id="HEX2704434.1"
                     /translation="MRHSADRPAPDTGGGAEPIDYSDRNLTLDAAEDRWAWRARLRAN
                     PHSLRIYRGAVFAVGLVVVVVGLIAVPAPGPGWLIVLGGVAVWASEFEFAQRLLDWGG
                     ARLHVWTTWMAASPWWVKVSVSLGTMAAVAAGVWALFAVTAVPGFLPDTVDAWLRSLP
                     GIE"
     gene            2894..2967
                     /locus_tag="VHM65_01650"
     tRNA            2894..2967
                     /locus_tag="VHM65_01650"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2928..2930,aa:Val,seq:cac)
BASE COUNT          431 a         1127 c         1183 g          503 t
ORIGIN      
        1 cgcgctcact agctggctgt aggccgagtc aagttgaggt gtgggcgtgt ggcatcggcc
       61 ccgcgtcgcg gagctgtttt tggattctgg ccggtggtgt tgttgctgga ggttgcctgg
      121 cgcccacgct gacttatgcg acctcaccgg ggtgggtcat ctggcctcgt tcgggctgcc
      181 gtgtggcgaa ggacccgggc gccaagctag gagcggcgta acacctttgg gggtgtccgc
      241 catagccgga tcggggtctg gtccgggtcc ttcgtgcctt cggcatggca ctaccgaccg
      301 cagttgtgaa cacggcacgg gtggcaagga ccgctgggga tggtgacctc gggggccgtt
      361 gctcgagggc catggtgtgc ccgggcgtgc tcatcgccac cgggcccgcg tcgctgtgcg
      421 gccgccgcga cagccgcgac cgccgcacag cctaggatgc gcggatggac gtggcacggt
      481 tcgggacccg cgaggcccgc cgagtcgtgc agatccggta tggcgcggcg gctctggacg
      541 cgctggacac gacaggtctc tgggcggtgg ttgccacctt cgaaggtgag gtcgtcgcgg
      601 cgcagctcgc cgacgtcagc ccggtcgagg cgccggtcgc agatccggcc ggctgggcgc
      661 cgctggacgg atcgtggagt acgacgctcg acgcgacggc ataccggctg gccgtccagg
      721 aggtccgcgg ccgcgttgcc gccggggcgg tctaccagtc caacatctgc cgggtcatga
      781 cgcacccgct ctcggccgat gccgaccttg gcgggctggc tgcccttctg cgccaaggga
      841 accccgcgcc atacgcgagc cgggtcaagg tcgccgaagc ggacctcgac atcgtctgcg
      901 cctcgccgga gctgttcctg agccgacgtg ggacacgtct ggcttcgtcc cccgtcaagg
      961 gcaccgcggc tccgggcgcg ccgatgctcg acaaggacta cgccgagaac gtcatgatca
     1021 ccgacctcgt tcgcaacgac ctgtcgcacg tctgcctccc agggacggtc gacgtcgagg
     1081 tcctgtgcgg cgccgaggac caccccggcc tgtcacacct cgtcaccacc gtggccgggc
     1141 ggctgcggcc ggacgcgacc tgggccaaga tcttcgcggc gaccttcccg ccgggctcgg
     1201 tctcgggggc ccccaagtcc agcgccctgc gggccatcgc cgacctcgag ccggggccgc
     1261 gagggcccta ctgcggagcg gtgggctggg tggacgccga cacacgcgag gcggaactcg
     1321 ccgtgggtat ccggacgttc tgggctgagc acgacatcag cggccggcgc tggctcaggt
     1381 tcggaaccgg ggccgggatc acctggggct ccgacccgca ggccgagtgg gaggagaccg
     1441 agctgaaggc tcgccgcctc gtggccctcg cctcgggcca cggccacgga gcgctcacga
     1501 agggacatcg gggatgacgc cggaggtcag ggtctgggtg aacggcggtc tcgtcgaggc
     1561 gggctcggcg gcgatcagcg ccctcgacca cggtgtcacg gtcggtgacg gcgtcttcga
     1621 gacgctcaag gtcgtcgacg gccgcccgtt cgccctgtcc cggcaccacc ggcggctggc
     1681 ccgctcgctg gccgggctgg gactgcccgg agccgacttc gaccacctcg ccgaggggat
     1741 cgaggccgtg ctggccggcg agccgatccc gttcgggcgg ctgcgctaca cggtcacggg
     1801 cggagcgggg ccgctcgggt cggaccgcca cgactgtgcg ctcacctaca tcgtcacggc
     1861 cggcccgtcg ccacggccgg cggactcagc tgctctgagc atcgtcccgt ggacccgcaa
     1921 cgaacgtgcc gcgaccgcgg ggctcaagac gacgtcatac gccgagaatg tgatggccct
     1981 ggcctacgcc aaggagcgtg gggcgctgga agccgtcttc gccaacaccc gcggcgagct
     2041 gtgcgagtgc acggggagca acatcttcgt cggcaccgac ggagtgctgc ggacgccccc
     2101 ggccgactcg gggctcctgc ccggcgtgac ccgcgagctc gtcatcgagt ggtgccgtca
     2161 ggccggcctg caggtcgccg aggaggccct gccgatcggt gtcctggccg acgcggacga
     2221 ggtcttcatc acctcctcga ccaaggacgt gctcggggtc cacgcggccg gccgctgacc
     2281 cgtcgggcgc agcagatctt cgctgcccgg gccgctcagg aggtggaccc atgaggcact
     2341 ccgcggaccg gccggcgccg gacaccggcg gcggtgccga gccgatcgac tactcggacc
     2401 gcaacctgac gctggatgcc gcagaggacc gctgggcctg gcgggcccgg ctgcgggcca
     2461 acccgcactc cttgcggatc taccgcggcg cggtgttcgc cgtcggcctc gtggtcgtcg
     2521 tcgtcggcct catcgccgtg ccggcccccg gtcccgggtg gctcatcgtc ctcggcggag
     2581 ttgccgtctg ggcctcggag ttcgagttcg cccaacgcct gctcgactgg ggcggggccc
     2641 gcctgcacgt ctggaccacg tggatggcgg cctcgccgtg gtgggtcaag gtcagcgtca
     2701 gcctcggcac gatggcggcc gtcgcggccg gcgtgtgggc gctgttcgcc gtcacggccg
     2761 tcccgggctt cctccccgac accgtcgacg cgtggctgcg gtcgcttcct gggatcgagt
     2821 gatccgcagg ggattggtgc tcggcctcgg ggggcggcta tccttcggtc cgttgcggca
     2881 ccaccgcggc tctggacgtg tagctcagct ggttagagcg ttcgcttcac acgcgagagg
     2941 tcatgggttc gagtcccttc acgtccactc cccctacagt cctttgacct gcgaaaacgc
     3001 cacggatcgg gcccactcct attcaacaac gtgtcggata cgtgtcgatg gtccgccttg
     3061 tcatcgggga gagccaccct gaccacagcg acaccagcca cccgggccgg accgaccaaa
     3121 ccgaccgcgg gtcgactggg gtccggcgtc gagccggtga tcgggatgtg gaccggcagc
     3181 cagaggcacc ggacacaggc cttgggccgg acgacgcctt gtggagcatc gaggacctgc
     3241 agag
//
LOCUS       DATAIX010000107         2507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_36176, whole genome shotgun sequence.
ACCESSION   DATAIX010000107 DATAIX010000000
VERSION     DATAIX010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36176"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1042)
                     /locus_tag="VHM65_01655"
     CDS             complement(92..1042)
                     /locus_tag="VHM65_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX2704435.1"
                     /translation="MDPSDLLRNGLLALSKSTAVRNAVERAPVSRNVVRRFVPGAGTE
                     DAVEAVGRLRVTGRTATIDYLGEDTLDPTQARHTRDAYLRLLAALCERDYTAGGAAEV
                     SLKLSALGQALPGDGPKAALEHAREICQAAAEAGTTVTLDMEDHTTTDRTLEALRELR
                     ADFPSVGAVLQSYLHRTEADCKDLAGAGSRVRLCKGAYKEPESVAYQDSGDVDRSYVR
                     CLKVLMSGQGYPMVASHDPRLVEIAGALAVQAGRDPKSFEYQMLFGIRPEEQKRIADR
                     GDQMRVYIPYGDEWYGYLMRRMAERPANAFFFLRGVATRG"
     gene            complement(1110..1844)
                     /locus_tag="VHM65_01660"
     CDS             complement(1110..1844)
                     /locus_tag="VHM65_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2704436.1"
                     /translation="MSPRLTLLTAGRVLRQLRGDRRTVALMLVVPCVLIGLLAWIYAD
                     TPIFDGIGPALLGVFPFVVMFIVTSVATLRERTSGTLERLLTTPLGKGDFMFGYALAF
                     GVVAVIQALIATAFAVYVCGLDIKGPLWVLILVAVLDAVLGTALGLLASGFARTEFQA
                     VQFMPAFVLPQFLLCGLIAPRDQLPGVLSAVSDVLPLSYAVDAMHTVTVSSAPLTDMA
                     SDLGIIIAWVLVSLGLGSLTLRRRTP"
     gene            complement(1841..>2507)
                     /locus_tag="VHM65_01665"
     CDS             complement(1841..>2507)
                     /locus_tag="VHM65_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2704437.1"
                     /translation="SVNVPTGQVTGLLGPSGSGKSTLLRAIVGVQLVASGEVRVLGDP
                     AGSPTLRRRVGYLTQEPSVYDDLSVRANVAYLAGVVGARAGAVQEAITSVDLDSHADV
                     LVGRLSGGQRSRVSLACALVGGPPLLVLDEPTVGLDPVLRRDLWNLFHRLADAGTSLL
                     VSSHVMDEATRCDRLLLLREGRLLSDATMPELLATTGAVDAEGAFLALIDGAAASPQG
                     DPR"
BASE COUNT          414 a          858 c          926 g          309 t
ORIGIN      
        1 gccgtgcccg ccaccggcgg gcacggcata ctgtgctgca cgggtgacgg ccctgctccc
       61 aggccccgct ccggggcccc tgcgcgggac cctacccgcg ggtggcgacc ccgcgcagga
      121 agaagaacgc gttggccggg cgctcggcca tccggcgcat gaggtagccg taccactcgt
      181 cgccgtacgg gatgtagacg cgcatctggt cgccgcggtc ggcaatgcgc ttctgctcct
      241 cggggcggat gccgaacaac atctggtact cgaacgactt cgggtctcgt ccggcctgga
      301 cggcgagcgc cccggcaatc tcgacgagcc gcgggtcgtg gctcgctacc atgggatagc
      361 cctgacccga catcagcacc ttgaggcagc gcacatagga gcggtcgacg tcgccggagt
      421 cctggtaggc caccgactcg ggctccttgt aggcgccctt gcacagccgg acccggctgc
      481 ccgcaccggc gaggtccttg cagtcggcct cggtccgatg caggtaggac tgcaggacgg
      541 ccccgaccga cggaaagtca gcccgcagct cgcgcagcgc ctcgagcgtg cggtcggtcg
      601 tcgtgtggtc ctccatgtcc agggtgaccg tcgtgccggc ctcggcggcg gcctggcaga
      661 tctcgcgagc gtgctcgagg gcggccttgg ggccgtcgcc gggcagggcc tgcccgagcg
      721 cgctcagctt gagcgagacc tcggcggccc cgccggcggt gtagtcccgc tcgcacaggg
      781 cagcgagcag gcgcagatag gcgtcgcgcg tatgccgggc ctgcgttggg tcgagggtgt
      841 cctcgcccag gtagtcgatc gtggccgtgc gtcccgtgac gcggagccgg ccaacggcct
      901 cgactgcgtc ctcggtcccg gcaccgggga cgaaacgcct gacgacgttt cggctcaccg
      961 gggcgcgttc gacggcgttg cgcactgccg tcgacttgga cagggcgagc aaaccattgc
     1021 ggagcaggtc gctggggtcc accaaaccaa tctagcgggg aggtcgccac ggtgccgacc
     1081 ccggcgcaac gaggtgaccg ggcaccaggt cagggagtcc ggcggcgcag ggtcaaggac
     1141 cccaggccca aggagacaag cacccacgcg atgatgatcc ccaggtcgct ggccatgtcg
     1201 gtgagcgggg cgctgctgac ggtcaccgta tgcatcgcgt cgacggcgta cgacaggggc
     1261 agcacgtccg acaccgctga cagcacaccc gggagctggt cacgcggcgc gatgaggcca
     1321 cacaggagga actgcgggag gacgaacgcg ggcatgaact gcaccgcctg gaactcggtg
     1381 cgggcgaaac cgctggcgag cagccccaac gcggtaccga gcacggcgtc gaggaccgcg
     1441 acgaggatga gaacccacag cgggcccttg atgtccagcc cgcagacata gacggcgaac
     1501 gccgtggcga tcagcgcctg gatgacggcg acgaccccga aggccagcgc atagccgaac
     1561 atgaagtcgc ccttaccgag cggcgtggtg agcaggcgct cgagcgtccc cgaggtgcgc
     1621 tcgcgcaggg tcgccacgct cgtgacgatg aacatgacga cgaacgggaa gacgccgagt
     1681 agggccggcc cgatcccgtc gaagatcggc gtatcggcat agatccaggc gagcaggccg
     1741 atgagcacgc agggcacgac gagcatgagc gccaccgtgc ggcggtcgcc ccggagctgg
     1801 cgcaggactc gcccggccgt gagcagggtg aggcgggggc tcatcgcggg tccccctgcg
     1861 gcgaggcggc tgcgccgtcg atgagcgcga ggaacgcccc ctcggcgtcg accgccccgg
     1921 ttgtcgcgag cagctctggc atcgtggcgt cggacagcag gcgcccctcc cggagcagca
     1981 ggagccggtc gcagcgggtg gcctcgtcca tgacatggct ggagacgagc aggctggtcc
     2041 cggcgtcggc gagccggtgg aagaggttcc acaggtcgcg acgcagaacc gggtcgagcc
     2101 cgaccgtcgg ctcgtcgagg accagcaggg gcggtccccc gacgagggcg caggcaaggg
     2161 acacccgaga gcgctgacca cccgagagcc ggccgacgag gacgtcggca tgactgtcca
     2221 ggtcgaccga cgtgatcgcc tcctggacgg cacccgcacg ggcgccgacc accccggcga
     2281 ggtaggcgac gttcgcgcgg accgacaggt cgtcatacac gctgggttcc tgggtgaggt
     2341 agccgacgcg gcggcgcagc gtcggcgacc cggcgggatc accgagcacc cggacctcgc
     2401 cgctggccac cagctggacg ccgacgatgg cccgcagcag agtggacttg cccgaccccg
     2461 acggtccgag caaaccggtg acctggccgg tcgggacgtt gacggac
//
LOCUS       DATAIX010000108         1689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_66069, whole genome shotgun sequence.
ACCESSION   DATAIX010000108 DATAIX010000000
VERSION     DATAIX010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66069"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..1485
                     /locus_tag="VHM65_01670"
                     /pseudo
     CDS             334..1485
                     /locus_tag="VHM65_01670"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1502..>1689)
                     /locus_tag="VHM65_01675"
     CDS             complement(1502..>1689)
                     /locus_tag="VHM65_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704438.1"
                     /translation="TWPAYEDRRLQAPGCELGGAAAPAATQFPSSRRCSRSRRGPSGF
                     TSFPVPHWKKIWSTNPQ"
BASE COUNT          257 a          560 c          624 g          248 t
ORIGIN      
        1 gactgtcgat gacggtgccg caatcggcgg tcgagccgcg gcatgccaat cagacaactc
       61 tctctcgact ctacgacctg actggcggaa tggtcgggcg gcatcgcgca gaagcgccgt
      121 tccggatcaa gcggtcgcca gcgggggacg ctcctcgcaa cggccagccg tgcggaactg
      181 ccgtggaatg tcgcgctgca ccgcctctcg gtgaacggat gcgctggacg ccgtggtcga
      241 tcacggtcga gtctgtcagc gtggtgacgt cgagggagca ggtgctcgag ctgtgcggcc
      301 gcgaggtggt gatcacgaac ccggacaagg gtcgtgttcc ccgacgccgg gcacaccaag
      361 ttggacctgg tccgttacta cttgtcggtt gccgaggggg cgctgcgcgg ggtcgccggc
      421 cggccgatga tcctcaagcg gttcgtcaag ggcatcggcg aggaggcggt gtttcagaag
      481 cgggccccag ccacgcggcc ggagtggttg gacgtcgctg agctgcatta cgcgtcgggg
      541 acctcggcgg ccgagatcgt ggtgcgggac gaggccgggc tggcttgggt catcaacctc
      601 ggttgcgtgg acctcaaccc gcacccagtg ttggctgagg acctcgagca ccccgccgag
      661 ctgcgcatcg acctggaccc ggtgcccggc gtcgagtggc agcagatcgt cgacgtcgcg
      721 ctcgtcgccc gggaggtcct cacggatgcg ggactatccg cgtgaccgaa gacgtccggc
      781 tcccgcggct tccacatcta tgccccggtc gacccggtct ggacctaccc ccaggtgcgg
      841 ttggcggcga aggccgtggc ccgcgaggtg gggctccgcg cacccgggct ggcgacgagc
      901 cgttggtgga aggaggaacg tcacggggtg ttcgtcgact tcaaccagaa cgccaaggac
      961 cgcacggtgg cttcggcgta ctcggtgcgg gccacgccgg acgcgcgggt gtccacgccg
     1021 ctgcgttggg acgaggtgcg ccaggtccgc ccggaggacc acacgctgac caccgtgccc
     1081 gcccggctcg ccgagatcgg cgacccgtgg gccgggatcg acgccgaacc cgggtcgctc
     1141 gaggcgctgc tcgagctggc cgagcggctc ggcccaatgg agaagcgccc gaaggggacc
     1201 gggcggcgaa cgctcagcat gccgctcgtc gaggtggttc gagcccgcac gaaaccggag
     1261 gccctcgccg gcctcgagtc ctggcagagg aagcaccccg acgtcgtgcc gctgctggag
     1321 gactctgacg tgctcgtcga tggcatgcgc ggcagcagct cactgtggta ccgcgtgcgg
     1381 atcaacctcc agcacgtccc ggaggcgcaa cgtcccgagc aggccgagct ggaggtcgac
     1441 tacgatccct gggcgggcta ccgcggctcc cagcgccggc gctgaaatga ccccgcccag
     1501 ctcactgcgg gttggtcgac cagatcttct tccagtgcgg caccgggaag gaggtgaagc
     1561 cagacggtcc tcggcggctc cgggagcagc gcctcgacga tgggaactgg gtggccgcag
     1621 gggccgccgc gccgccgagc tcgcaaccgg gagcttggag ccgccggtct tcgtaagccg
     1681 gccaggtgc
//
LOCUS       DATAIX010000109         3437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_6024, whole genome shotgun sequence.
ACCESSION   DATAIX010000109 DATAIX010000000
VERSION     DATAIX010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6024"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VHM65_01680"
     CDS             complement(<1..914)
                     /locus_tag="VHM65_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX2704439.1"
                     /translation="MSEDVRTEVTGPQARMRARLARLGTPRTNPALEPLIRTVRASHP
                     KADVSVIERAYAVAEQAHSGQTRRSGDAYITHPLAVTAILADLGMTPNTLAAALLHDT
                     VEDTSYSLEALKRDFGQEVAMLVDGVTKLDKVTYGEAAQAETVRKMVVAMARDIRVLV
                     IKLADRLHNARTWRYVSPESAARKAKETLEIYAPLAHRLGMNTIKWELEDLSFAALYP
                     KVYDEIVRMVAERAPAREEYLFGVCSEVERDLKAAKIRATVTGRPKHYYSVYQKMVGR
                     GREFDEIYDLVAVRVLVDSVRDCYAALGS"
     gene            complement(957..1487)
                     /locus_tag="VHM65_01685"
     CDS             complement(957..1487)
                     /locus_tag="VHM65_01685"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX2704440.1"
                     /translation="MSSESVRSLLARTIRDSPDFPTPGVTFKDFTPLLADAQAFRTVV
                     KDIADRYAGRVDVVAGIEARGFIIGAAVAYELGAGFVPIRKAGKLPGDTHRESYSLEY
                     GTAEIEMQTDALAPGVRVLVLDDVLATGGTAAAACALIERAGEQVVAVEAILELSFLH
                     GRRALPGREIHSLLAV"
     gene            complement(1498..2622)
                     /gene="secF"
                     /locus_tag="VHM65_01690"
     CDS             complement(1498..2622)
                     /gene="secF"
                     /locus_tag="VHM65_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX2704441.1"
                     /translation="MNFAQFGNDLYTGKRSVDFIGRRTTWYLVSAVLIALAALGLVSR
                     GLNFGIEFRGGSEFRVSAVPSTSDYEAKAKAAISTAGIPGIVTTTVVGGSSVRVQTEA
                     ADTKTEAAKTALAKAFGVDPASVSSALIGPSWGASVSSKAIQALIVFLILVSLVMAAY
                     FRTWKMAVAGLVALVHDLLITVGIYALFGFEITPSSMIGFLTILGYSLYDTVVVFDKV
                     RENTAEAMTGRRSSYADAANLAVNQTLVRSINTTVVALLPIAAILVVGFTLLGPGTLL
                     DLSLALFVGIAVGAYSSIFIATPLLVALRNTEPAVKELAKAAARHQERHVPVPAADSA
                     RPGPGEIQDGAGTPAAARPVHKYASAGPRNQPRRPPRSKR"
     gene            complement(2624..>3437)
                     /locus_tag="VHM65_01695"
     CDS             complement(2624..>3437)
                     /locus_tag="VHM65_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SecD/SecF family protein translocase subunit"
                     /protein_id="HEX2704442.1"
                     /translation="NRFAIVLDRAVITAPTTEAAIVTGQASITGSFTLDSARVLARQI
                     KFGALPLSFKVQTEETISPQLGDEQLRLGLLAGLIGLLLVVFYSLLQYRALGLVTVAS
                     LVVAAVLTFGILTFLGWAYNFRLTMAGVTGVIVSIGVTADSFIVYFERIRDEVRDGRP
                     LVAAVEAGWARAKRTILAADAVNFLAAAVLYLLAANNVRGFAFTLGVMTLIDLLVVFL
                     FTHPTVGLLARTEFFGGGHKWSGLDPERLGSAGPRYVGRGRFAGPQIAEGKA"
BASE COUNT          543 a         1122 c         1268 g          504 t
ORIGIN      
        1 gagccgagcg cggcgtagca gtcccgcacc gagtcgacga ggacccggac ggcgacgagg
       61 tcgtagatct cgtcgaactc gcggccacgc ccgaccatct tctggtagac ggagtagtag
      121 tgcttggggc gcccggtcac ggtcgcgcgg atcttggcgg ccttgaggtc gcgctcgacc
      181 tcgctgcaga ccccgaagag gtactcttcc cgggctggcg cccgctcggc caccatccgc
      241 acgatctcgt cgtacacctt ggggtagagc gccgcgaagg agagatcctc gagctcccac
      301 ttgatggtgt tcatgccgag ccggtgagcc aacggggcgt agatctccag ggtctccttg
      361 gccttgcgtg ccgccgactc cggcgagacg tagcgccagg tcctcgcgtt gtggagccgg
      421 tcggcgagct tgatgacgag cacccggatg tcgcgcgcca tggcgacgac catcttgcgc
      481 acggtctcgg cctgcgcggc ctcgccgtag gtgaccttgt cgagcttggt cacgccgtcg
      541 acgagcatgg cgacttcctg cccgaagtcg cgcttgagcg cctccaggct gtatgacgtg
      601 tcctccacgg tgtcgtggag cagggccgcg gccagcgtgt tgggcgtcat gcccaggtcc
      661 gcgaggatcg ccgtcaccgc gagcgggtgg gtgatgtagg cgtccccgct acgccgggtc
      721 tggcccgaat gcgcctgctc ggcgacggca taggcccgct cgatgactga gacgtccgcc
      781 ttggggtggc tcgccctgac cgtgcggatg agaggttcga gcgccgggtt cgtccggggt
      841 gtgccgagcc gggcaaggcg ggcgcgcatg cgggcctgag gtccggtgac ctccgtccgc
      901 acgtcctcgc tcatggcggc agtgtagtcg cgtcgccggc ccgcccaggc gggactctag
      961 accgcgagca ggctgtggat ctcgcggccc ggcagggcac ggcgcccgtg caggaacgag
     1021 agctcgagga tggcttcgac ggcgacgacc tgctctcctg ctcgctcgat cagggcgcag
     1081 gctgcggccg cggtgcctcc cgtcgccagg acgtcgtcca gaacgaggac ccggacgccc
     1141 ggggccaggg cgtcggtctg catctcgatc tcggcggtgc cgtactccag ggagtaggac
     1201 tcccggtggg tgtcccccgg cagcttgccc gccttgcgga tcgggacgaa ccccgcgccg
     1261 agctcatagg ccacggcggc tccgatgatg aagccacggg cctcgatccc cgcgacgacg
     1321 tccacacgcc cggcatacct gtcggcgatg tccttgacga ccgtccggaa tgcctgcgcg
     1381 tcggcgagca acggcgtgaa gtccttgaac gtgacccccg gggtggggaa gtccggggag
     1441 tcacggatcg tccgggcgag gagggaccgg accgactcgc tgctcatcgc tgcgggctca
     1501 ccgctttgag cgcgggggac gcctcggctg gttgcgcgga cccgccgagg cgtacttgtg
     1561 caccggacgt gccgccgccg gcgtgcccgc gccgtcctgg atctcgcccg gacccggccg
     1621 ggctgagtcc gccgcgggga ccgggacgtg acgctcctgg tggcgcgcgg cggccttcgc
     1681 gagctccttg accgcgggct cggtgttgcg cagcgccacg aggagcgggg tcgcgatgaa
     1741 gatcgaggag taggcaccga cggcgatccc gacgaacagc gccaggctga ggtcgagcag
     1801 ggttccgggg ccgagcagcg tgaagccgac gacgaggatc gccgcgatgg gcaggagcgc
     1861 gacgacggtc gtgttgatcg agcggaccag ggtctggttc accgcgaggt tcgcggcgtc
     1921 ggcatacgag ctgcgccgtc ccgtcatggc ctcggcggtg ttctcgcgga ccttgtcgaa
     1981 gacgacgacc gtgtcgtaga gcgagtagcc gagaatggtg aggaagccga tcatcgacga
     2041 cggggtgatc tcgaagccga agagcgcgta gatgcccacg gtgatgagca ggtcgtgcac
     2101 gagcgcgacg agaccggcga cggccatctt ccacgtgcgg aagtaggccg ccatgaccag
     2161 ggacacgagg atgaggaaga cgatgagcgc ctgtatcgcc ttgctgctga ccgacgcgcc
     2221 ccacgagggg ccgatgagcg ccgagctgac gctcgccggg tcgaccccga aggccttggc
     2281 cagagccgtc ttggcggcct cggtcttggt gtcggcggcc tcggtctgga cgcggacact
     2341 ggagccaccg acgaccgtgg tcgtcacgat cccggggatg cccgccgtgc tgatggctgc
     2401 cttggccttg gcctcgtagt ccgaggtgct ggggacggcg gacacccgga actccgagcc
     2461 accccggaac tcgatgccga agttgagccc ccggctgacc aggccgaggg ccgccagcgc
     2521 gatgagcacc gccgacacga ggtaccacgt cgtgcggcga ccgatgaagt cgaccgagcg
     2581 cttgccggtg tagaggtcgt tcccgaactg ggcgaagttc atgctaggcc ttcccctcgg
     2641 cgatctgggg cccggcgaac cggccgcggc cgacataccg tggaccggcc gagccgagcc
     2701 gctccggatc gagcccggac cacttgtgcc cgccgccgaa gaactccgtg cgcgccagga
     2761 ggccgaccgt cgggtgggtg aagaggaaca cgacgagcag gtcgatgagc gtcatcaccc
     2821 cgagggtgaa ggcgaaccca cggacgttgt tggccgcgag caggtagagc acggcggcgg
     2881 cgaggaagtt cacggcatca gcggccagga tcgtgcgctt ggcccgggcc cagcccgcct
     2941 cgactgccgc gacgaggggc cggccgtccc gaacctcgtc ccggatgcgt tcgaagtaga
     3001 cgatgaacga gtccgccgtg acaccgatcg agacgatgac accggtgacc ccggccatgg
     3061 tcaggcggaa gttgtaggcc cacccgagga aggtcaggat gccgaaggtc agcacggcag
     3121 ccacgacgag cgaggccacg gtgaccagtc ccagggcgcg gtactgcagc agcgagtaga
     3181 agaccacgag aagcaggccg atgagaccgg ccagcaggcc caggcgcagc tgctcgtcac
     3241 cgagctgggg gctgatcgtc tcctcggtct ggaccttgaa ggacagcggg agcgcaccga
     3301 acttgatctg ccgggcgagc acccgggccg agtcgagggt gaagctgccg gtgatgctcg
     3361 cctgaccggt gacgatcgcg gcctcggtgg tcggtgcggt gatcacggcg cggtcgagca
     3421 cgatggcgaa ccggttg
//
LOCUS       DATAIX010000110         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_155529, whole genome shotgun sequence.
ACCESSION   DATAIX010000110 DATAIX010000000
VERSION     DATAIX010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155529"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VHM65_01700"
     CDS             <1..466
                     /locus_tag="VHM65_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX2704443.1"
                     /translation="LASTPGDLVIMQFGHNDQKIPTLAPREGFARNLERFVEECRERG
                     QRPVLCTPVARRRFVEGRLVPTLGEYPEAVLELGARLDVPVVDLHAVTSRLYEAWGDE
                     GSRVLFTQLLADQHPLYRIGIADDTHFSITGAQAVAHEVAAALVPLIEEARA"
     gene            463..>2187
                     /locus_tag="VHM65_01705"
     CDS             463..>2187
                     /locus_tag="VHM65_01705"
                     /inference="COORDINATES: protein motif:HMM:NF044450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704444.1"
                     /translation="MTAPVRRVTGTGSVTWGVPWPRGQVSSDASFRLGEVPVQSWPLA
                     TWPDGSLKWSGHATSLVGSGTLEVGEPATPDEPVVVDEGQEGVTVSVGTHVWHIARTG
                     ALVDQVLVDGRLVAGPISLVSQRASQAGPGDREDWVGHVTDMVVEQSGPVRAVVRLRG
                     AHRQIEARQTREWLPFTVRLVWFADTDEVRIMHSFVWDGDPESDFLAALGVQVGVPMV
                     DDPTDRHIRIAGPMREGGPGFCTEAVRGITGLRRDPGREARASQIAGRPVGELSEPVA
                     GLLHWVPTWDAWRLDQLSPDGFTLAKRTAPDRPWVTIPGGTRAAGLAYLGGVSGGVAL
                     GVRDFWRSCPASLEIAGAAGDNGRLTAWLWSPRAEPMDLHAWHDDLGTHGWGDEVEAM
                     EITYEDYEPGFPSPHGIARTHELRLVAYAATPAHDVLAEAAQRTDDPGVAAAEPAYLR
                     DCRVFGDWDLPDRSSREAAAIEDRLDWFFEYYVTQREERRWYGFWDYGDVMHTYDPDR
                     HQWRYDVGGFAWDNSELSPDLWLWYQFLRTGRADVYRFAEAMTRHTGEVDVYHLGRFA
                     RLGSRHNVQ"
BASE COUNT          309 a          764 c          789 g          325 t
ORIGIN      
        1 cctggcctcg acgcccgggg atctcgtgat catgcagttc ggtcacaacg accagaagat
       61 cccgacgctc gcgccacgcg aggggttcgc ccgcaacctc gagcggttcg tcgaagagtg
      121 ccgggagcgg ggccagcggc cggtgctgtg tacaccggtg gcgcggcgtc ggttcgtgga
      181 gggccggctc gtccccactc tcggggagta tcccgaggcc gtcctcgagc tcggagcccg
      241 gctggacgtg cccgtcgtgg acctccatgc ggtgacctca cgcctctacg aggcgtgggg
      301 cgacgagggc tcgcgagtcc tgttcaccca gctcctcgcc gaccagcacc cgctctaccg
      361 gatcgggatc gccgacgaca cccacttcag catcaccggg gcccaggcgg tggcgcacga
      421 ggtggccgcc gcgctggttc ccctcatcga ggaggcacga gcatgacagc acccgtccgt
      481 cgcgtcaccg gtaccggatc ggtcacctgg ggagtgccgt ggccacgggg gcaggtcagc
      541 agcgacgctt ccttccgcct cggggaggtc cccgtgcagt cctggcccct ggccacgtgg
      601 ccggacggtt ccctgaagtg gagcggccac gccacgtcgt tggtcgggtc cgggactctg
      661 gaggtcggtg agccggccac ccctgacgaa ccggtcgtcg tcgatgaagg gcaggagggg
      721 gtgacggtga gcgtcgggac gcatgtctgg cacatcgccc ggaccggcgc cctcgtcgac
      781 caggtgctcg tggacggacg cctggtggcc ggacccatca gcctcgtgag ccaacgggcg
      841 tcccaggcag ggccgggcga ccgggaagac tgggtcggcc acgtcacgga catggtggtc
      901 gagcagtccg ggccggtgcg ggcggttgtc cgcctgcggg gcgcccaccg acagatcgag
      961 gcgcgccaga cccgcgagtg gctcccgttc accgtccgac tggtctggtt cgccgatacc
     1021 gacgaggtcc ggatcatgca ctcgttcgtg tgggacggcg acccggagag cgacttcctc
     1081 gccgcgctcg gcgtacaggt cggcgtgcct atggtcgacg acccgacaga tcgtcacatc
     1141 cgcatcgccg gaccgatgcg tgagggtgga cccgggttct gcaccgaagc cgtcagaggc
     1201 atcacaggac tgcgccgcga tccgggtcgg gaagcgaggg cgagccagat cgccggacga
     1261 ccggtcggcg agctcagcga gccggtggcg ggactgctcc actgggttcc cacctgggac
     1321 gcctggcggc ttgatcagct ctccccggac ggcttcacgc tggccaagcg caccgctccc
     1381 gaccgtccct gggtgacgat cccgggcggg acgcgagcag cgggtctggc atacctcggc
     1441 ggggtctccg gcggtgtcgc cctcggcgtc cgcgacttct ggcgatcctg ccccgcctcg
     1501 ctcgagatcg cgggggccgc cggcgacaac ggccgactga ccgcctggct gtggtccccc
     1561 cgggccgagc cgatggacct ccacgcgtgg cacgacgacc tggggaccca cgggtggggc
     1621 gacgaggtcg aggccatgga gatcacgtac gaggactacg agcccggctt cccctcgccg
     1681 cacgggatcg cccggaccca cgagctgcgt cttgtcgcct acgcggcgac gcccgctcac
     1741 gacgtgctgg ccgaggcggc gcagcgtacc gacgacccgg gtgtggcggc cgcggaaccc
     1801 gcgtacctgc gcgactgccg cgtgttcggc gactgggacc tgccggaccg ctcctcccgc
     1861 gaggcggcgg cgatcgagga caggctcgac tggttcttcg agtactacgt cacccagcgt
     1921 gaggagcggc gctggtacgg gttctgggac tacggcgacg tgatgcacac ctacgacccc
     1981 gaccgccacc agtggcgtta cgacgtcggt ggcttcgcct gggacaactc ggagctctcc
     2041 cccgacctgt ggctctggta ccagttcctg cgaaccggcc gggcggacgt gtatcgcttc
     2101 gccgaggcca tgacgcggca cacgggcgag gtggatgtgt accacctcgg gcgtttcgcg
     2161 cgtctcggct cgcggcacaa cgttcag
//
LOCUS       DATAIX010000111         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_36298, whole genome shotgun sequence.
ACCESSION   DATAIX010000111 DATAIX010000000
VERSION     DATAIX010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36298"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VHM65_01710"
     CDS             <1..832
                     /locus_tag="VHM65_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEX2704445.1"
                     /translation="QNQVQAMVTKGAKVLVVGAIDGSQLGTQLKAAKQAGATVIAYDR
                     LVKNTDAVDYYVAYDNFKVGVLQANALLEGMAAKKPAGPYNIELFAGSPDDANAQVFF
                     DGAMSVLKPKIADGTLKVVSGQADFNKAVTQGWLKDNAQKRMDTLLAGNYTKTELDGV
                     LSPNDNLARAVLTSVKAAGKPIPVVTGQDSEVESVKSIMAGEQYSTINKDTRALVKAT
                     IEMVKALQKGGKPTVTDEKSYNNGVKVVPANLLVPIIVTKANAKEAYANDPTLSKLTM
                     "
     gene            complement(1087..>2368)
                     /locus_tag="VHM65_01715"
     CDS             complement(1087..>2368)
                     /locus_tag="VHM65_01715"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEX2704446.1"
                     /translation="LVAQRHDRVRQEDPARAVGALGDDADVADVARAGIDERAAEAFG
                     RRGADRPDESGAGAAEDGQVRPADPGAGALEALRPGAAPLGGPSPALVWVVTAMSAEG
                     LSRTLVDARTGEVRDVRVISKGADGTGRVFLPNPVVTLRDESLVDKGDRNQVALRPAY
                     QDVVLSHLDGNGRLTGSAVRIVRARGGLAKSATSTFGYLRADDRFEQVNAYYAVDISQ
                     TYLQRLGFTDANNEAQRVAINTYAGDNSFYDPDKDLITFGLGGVDDAEDAEVIWHEYG
                     HAIQDAIVPGFGSTEEAGAIGEGFGDYWAVTMSVPSSRGFDLPCVMDWDATSYTSGPS
                     HCLRRVDGVKTVADMDGEVHDDGEIWSRALWDIHRGLGRAKANRVVIESTYFFSPDTT
                     FASAARQTVQAARALYGADAAAVVSRAFQDRGIL"
BASE COUNT          412 a          830 c          787 g          339 t
ORIGIN      
        1 gcagaaccag gtccaggcca tggtgaccaa gggcgccaaa gtcctcgtcg tgggggccat
       61 cgacggctct cagctcggca cgcagctcaa ggccgccaag caggccggcg caacggtcat
      121 cgcatacgac cggctcgtca agaacaccga cgcggtcgac tactacgtcg cctacgacaa
      181 cttcaaggtc ggcgtgctcc aggccaacgc cctgctggag ggtatggcgg ccaagaagcc
      241 ggccggtccg tacaacatcg agctgttcgc cgggtcaccc gacgacgcca acgcgcaggt
      301 gttcttcgac ggcgccatga gcgtcctgaa gccgaagatc gccgacggca ccctcaaggt
      361 cgtctcgggt caggccgact tcaacaaggc ggtcacccag ggctggttga aggacaacgc
      421 ccagaagcgc atggacaccc tcctcgcggg caactacacc aagaccgagc tcgacggtgt
      481 gctgtccccg aacgacaacc tggcccgcgc ggtcctcacg tcggtcaagg cagcaggcaa
      541 gccgatcccg gtcgtcaccg gccaggactc agaggtcgag tcggtgaagt cgatcatggc
      601 gggcgagcag tactcgacga tcaacaagga cacccgtgcc ctcgtcaagg cgaccatcga
      661 gatggtcaag gcgctccaga agggtggcaa gccgaccgtc accgacgaga agtcgtacaa
      721 caacggcgtc aaggtcgtcc cggccaacct cctggtaccg atcatcgtca cgaaggccaa
      781 cgccaaggag gcctacgcca acgacccgac cctgtccaag ctgaccatgt gaggacaggt
      841 tccctgacct gacccatcag gtcgacgaag gggccccggc cgaccaggcc ggggcccctt
      901 ctgcctgctc acccgacttc ctgggccggg cccggaccgc ccgacaggac caacgtctgg
      961 gagtgccccc gagaatagcg ccaaaccagg gcataagccg ccgtgcgagc ggctttgacc
     1021 agttctccgg tccgctggag ccaccgatga ggtatggcgg cgtcacgcga gccacgccgc
     1081 gcgggctcag aggatgccgc ggtcctggaa ggctcggctg accacggcgg cggcgtccgc
     1141 gccatacaac gccctggctg cctggaccgt ctgtctcgcg gcggaggcga acgtcgtgtc
     1201 ggggctgaag aagtacgtcg actcgatgac gacgcggttg gccttggctc gcccgagccc
     1261 gcggtggatg tcccacagcg cgcgtgacca gatctcgccg tcgtcgtgca cctcgccgtc
     1321 catgtcggcg accgtcttga cgccgtcgac gcggcgcagg cagtgcgagg gaccagaggt
     1381 gtatgacgtc gcgtcccagt ccatgacgca cggcaggtcg aacccgcggc tcgagggcac
     1441 cgacatcgtc accgcccagt agtcgccgaa gccctccccg atcgctccgg cctcctcggt
     1501 cgagccgaag ccgggcacga tggcgtcctg gatcgcgtga ccgtactcgt gccagatcac
     1561 ctcggcgtcc tcggcgtcat cgaccccacc gagcccgaag gtgatcaggt ccttgtccgg
     1621 gtcgtagaac gagttgtccc cggcgtaggt gttgatcgcg acgcgctgtg cctcgttgtt
     1681 ggcatcggtg aagccgagcc gttgcaggta ggtctgcgag atgtcgacgg cgtagtaggc
     1741 gttgacctgc tcgaaacggt catcggcgcg caggtagcca aaggtgctgg tcgcgctctt
     1801 ggccagtcca ccccgggccc gcacgatccg caccgcggag ccggtcagcc tgccgttgcc
     1861 gtcgaggtgg gagagcacga cgtcctggta tgccgggcgc agcgccacct ggttgcggtc
     1921 acccttgtcg accaggctct cgtcgcgcaa cgtcacgacc gggttcggca ggaagacccg
     1981 gcccgtgccg tcggcgccct tggagatgac gcggacgtcg cggacctcgc ccgtgcgggc
     2041 atcgacgagc gtgcggctga ggccttcggc cgacatggcg gtgaccaccc agacgagcgc
     2101 agggctgggg ccgcccagcg gcgcggcacc gggccgaagg gcttccaggg cgccagcacc
     2161 cggatcagcg gggcgcacct ggccgtcctc ggcggccccc gccccgctct cgtctgggcg
     2221 atcagcgcca cgtcgaccga aggcctcagc cgcacgctcg tcgatgcccg cacgggcgac
     2281 gtccgcgacg tccgcgtcat ctccaagggc gccgacggca cgggccgggt cttcctgccg
     2341 aacccggtcg tgacgttgcg cgacgaga
//
LOCUS       DATAIX010000112         4613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_241225, whole genome shotgun sequence.
ACCESSION   DATAIX010000112 DATAIX010000000
VERSION     DATAIX010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241225"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1251)
                     /locus_tag="VHM65_01720"
     CDS             complement(85..1251)
                     /locus_tag="VHM65_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX2704447.1"
                     /translation="MSTTDTRAVHAGREDLAARGVHVPSIDLSSTYPLPSVERGGDSY
                     ATLAGGGRPGPDDTFVYQRLWNPNVDRFERALAELEGTPEAVAFASGMAALTATLLAT
                     VTAGRPHLVAIRPLYGGTDHVIDSGLLGTTVTWATPDTVAASLRPDTGLVILETPANP
                     TLDLVDIEAVVAQAGAVPVLVDNTFATPVLQQPRRHGATLVLHSATKFLGGHGDLLGG
                     IVATTPEWATRIRHIRALTGGILYPLAAYQLHRGLQTLPVRVRAQQESAQAVATWLAG
                     HRAVSRVFYPGLEGSDPQGLLGRQMRGAGSILAIELRGGYDAASTTANGCCLITHAVS
                     LGGVDSLIQHPAALTHRPVAPPARPAASVLRLSIGLEDPQDIIDDLDKALAPTA"
     gene            1360..1863
                     /locus_tag="VHM65_01725"
     CDS             1360..1863
                     /locus_tag="VHM65_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX2704448.1"
                     /translation="MPKEARSLPRGPAPRSAPALDEVDRVLAEALAEDGRTPNATLAR
                     RAGIAESTCVGRVRALHDRGVIRGIHADLDPARVGLAVQAMVAVRFGGHVRAHVEAFR
                     DEVARLPGVIATYNISGANDYLVHVAAASPDALRDFVLDHLTNRPGVVHAETSLIFEA
                     TRGTRLL"
     gene            complement(1860..2240)
                     /locus_tag="VHM65_01730"
     CDS             complement(1860..2240)
                     /locus_tag="VHM65_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX2704449.1"
                     /translation="MSRPDPIYLTTEDLLSIADELGGLVVSDWGLLDSAAHRPAASAF
                     GEDAYPDLHLKAAVLLESIARNHALVDGNNRLAWVSTVVFLGLNGVRLVPPEDPAYDL
                     VIAVCETRINAKEVAAALVRWSAS"
     gene            complement(2237..2440)
                     /locus_tag="VHM65_01735"
     CDS             complement(2237..2440)
                     /locus_tag="VHM65_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704450.1"
                     /translation="MAINVRLSPEAEQVLTDLAAAEGLSKNDVINRAILEKGARAIRS
                     RDVRDLARAAIADYATLLDRLAQ"
     gene            2621..3469
                     /locus_tag="VHM65_01740"
     CDS             2621..3469
                     /locus_tag="VHM65_01740"
                     /inference="COORDINATES: protein motif:HMM:NF019359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2704451.1"
                     /translation="MAKASTETTTGMQTTGRVTVATHTYTVGQVAELFDVTVRTLHHY
                     DEIGLLTPSDRSSAGYRLYTDADLVRLQHIVVYRRLAFPLGQIASILDSDGPDVAAHL
                     RRQRAAVMSRLDEMRELVAAIDRALEKQMNDQPLTTAEMKELFGDGFRDEYQAEAEQR
                     WGDTEAWKQSSARTSRYTKADWEAIRADQEATGRALVEALTSGAPADSAAAMDAAEGA
                     RQHITRWFYDCPHAFHTNLADMYVADPRFTRTYEDLAPGLAQYVRDAIYANAARHGAS
                     GPPPGA"
     gene            complement(3485..4507)
                     /locus_tag="VHM65_01745"
     CDS             complement(3485..4507)
                     /locus_tag="VHM65_01745"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX2704452.1"
                     /translation="MTGDVLLHGPVVSQARRDAGLPGARATHGLDFRPLLAASRRVVE
                     GADLAICQLETPLAKPGGPYSGYPTFQVPQDILPALTDTGYDACTTASNHSVDAGWTG
                     LVRTVDALDSAGLKHTGTFRTQREADTPLLLDANGVKVALLNYAYGLNGLSEPAGRRY
                     AVNLISVPAILADAKAARRAGADVVLVALHAGNEYVQAPSAQQRAVVAALTASPDVDL
                     VYGHHVHVVQPFDRVNGKWVAYGLGNLIHNQAHSGNEPTRAEVAARFTFTERAAGGFA
                     VSKAEYIPGSMSIGTPMRYVDLGAALSNPSVPAATKARYRAVQAKVVQAVTSLGADEK
                     GLVLGR"
BASE COUNT          667 a         1546 c         1760 g          640 t
ORIGIN      
        1 ggaatcggtc ccatgggcaa gcgctccgac ctcgcgagga tagaggggcc gggccggcca
       61 gggcgcggcc ggagggggcg gcggtcaggc cgtcggagcc agcgccttgt cgaggtcgtc
      121 gatgatgtcc tgggggtcct cgagcccgat cgagaggcgc agcaccgacg cggccgggcg
      181 ggccggcggt gccaccgggc ggtgggtcag tgccgcggga tgctggatca gcgagtccac
      241 cccgccgagg gagaccgcgt gggtgatgag gcagcagccg ttcgccgtgg tggaggcggc
      301 gtcatacccg cccctgagct cgatggccag gatggacccg gcgccgcgca tctgcctgcc
      361 gagcagcccc tgcgggtcgc tgccctccag gccggggtag aagacgcggc tcaccgcccg
      421 gtgcccggcg agccaggtgg cgacggcctg ggcgctctcc tgctgggcgc ggacgcggac
      481 ggggagcgtc tgcaggccgc gatggagctg gtaggccgcg agcgggtaga ggatcccgcc
      541 ggtgagggcg cggatgtggc ggatccgcgt ggcccactcg ggcgtcgtgg ccacgatgcc
      601 gccgagcagg tcgccgtgtc cgccgaggaa cttcgtcgcc gagtgcagga cgagcgtcgc
      661 gccgtgccgc ctcggctgct gcaggaccgg cgtggcgaag gtgttgtcga cgagcacggg
      721 cacggctccc gcctgggcga cgacggcctc gatgtcgacg aggtccaggg tcgggttggc
      781 cggggtctcc aggatgacga gccccgtgtc gggccgcagg ctcgccgcca cggtgtctgg
      841 cgtcgcccag gtcaccgtcg tgccgagcag cccggagtcg atgacatggt cggtgccgcc
      901 gtagagcggc cggatcgcaa cgaggtgagg ccggcccgcc gtgacggtcg cgagcagggt
      961 cgcggtgagg gcggccatgc cggaggcgaa ggcgacggcc tccggggtgc cttcgagctc
     1021 ggcgagggcc cgctcgaagc gatcgacgtt ggggttccac aggcgctggt agacgaaggt
     1081 gtcgtcgggg cctggacgcc cgccgcccgc cagcgtcgcg taggagtctc cgccgcgctc
     1141 gacgctcggc agcgggtagg tcgacgagag gtcgatcgac ggcacgtgga cgccgcgggc
     1201 ggcgaggtcc tcacggccgg cgtggacggc gcgggtgtca gtagtgctca taccatcagg
     1261 gtgaggcttc tacggaatca cggcaacaga tccgcagaaa acatcccacg gacagaatgg
     1321 accgtggata ctgcgactct ggcgggtaga gtgagacgta tgccgaagga agcacgctcc
     1381 ctgccgcggg ggccggcacc acggtctgcc ccggcgctcg acgaggtcga ccgggtgctg
     1441 gccgaggccc tagccgagga cgggcggacc ccgaacgcga ccctggcgcg gcgcgccggg
     1501 attgccgagt cgacctgtgt gggacgcgtc cgggcgctgc acgaccgggg cgtcatccgg
     1561 ggcatccacg ccgacctcga ccccgcgcgg gtcggtctgg ccgtccaggc catggtggcc
     1621 gttcgtttcg gcggtcacgt ccgcgctcac gtcgaggcgt tccgcgacga ggtggcccgg
     1681 ctgcccgggg tgatcgcgac ctacaacatc agcggtgcca acgactacct cgtgcacgtc
     1741 gctgcggcct ccccggatgc cctgcgcgac ttcgtgctcg accacctgac gaaccgtccc
     1801 ggggtcgtcc acgccgagac gagcctgatc ttcgaggcca cccgcggcac ccgcctgctc
     1861 tagctcgcag accagcgaac cagggcggcg gcaacctctt tggcgttgat ccgcgtctcg
     1921 cagacggcga tgaccaggtc gtaggccggg tcctccggcg gcaccagccg gacgccgttg
     1981 aggccgagaa acaccacggt cgacacccag gccaggcgat tgttgccgtc cacgagggcg
     2041 tggttgcggg cgatcgactc caagagcacg gctgccttga ggtgcaggtc ggggtaggcg
     2101 tcctcgccga acgctgacgc cgccgggcgg tgagcagcgg agtcgagcaa cccccagtcg
     2161 ctgacgacca gtccaccgag ctcgtcggcg atgctcagaa ggtcctcggt ggtcaggtag
     2221 atcgggtcgg ggcggctcac tgggccaggc ggtcgagcag cgtcgcgtag tcggcgatgg
     2281 cggcgcgggc aaggtcgcgc acgtcacggg acctgatcgc gcgggccccc ttctccagga
     2341 tcgctcggtt gatcacgtca ttcttgctca agccctcggc cgcggcgagg tcggtgagca
     2401 cctgctccgc ttcggggctg agccggacgt tgatggccac gactgtcatg gtaccagtgt
     2461 ggcaccacgg cccgggatgg gctggcgctc atccacagcc tcgattatcc ccggcctcaa
     2521 tgaccggcgg gaggtgagcc ggccgtcata ctggctggtc tgagcttcgg cggttcattc
     2581 gcttgaccct cacgcggcgt gaggccgcac gctcggtgtc atggccaagg cgagcaccga
     2641 gacgacgact ggcatgcaga cgacaggacg ggtcacggtg gcgacacaca cctacaccgt
     2701 ggggcaggtg gccgagctgt tcgacgtcac cgtccggacg ctgcaccact acgacgagat
     2761 cgggctgctc acgccgagcg accgctcgtc ggccgggtac cggctctaca ccgacgccga
     2821 cctcgtccgg ctgcagcaca tcgtcgtcta ccgccggctc gccttcccgc tcgggcagat
     2881 cgcgtcgatc ctggacagcg acggccccga tgtcgccgcg cacctgcggc gccagcgggc
     2941 tgcggtcatg tcccggctgg acgagatgcg cgagctcgtc gcagccatcg accgagcatt
     3001 ggagaagcag atgaacgacc agcccctgac caccgccgag atgaaggagc tcttcggcga
     3061 cggcttccgt gacgagtacc aggccgaggc cgagcagcgc tggggtgaca ccgaggcgtg
     3121 gaagcagtcc tcggcgcgga cgagccgcta cacgaaggcc gactgggagg ccatccgagc
     3181 cgaccaggag gccaccgggc gggccctcgt cgaggcgctg acctctggag cgccggccga
     3241 ctcggcggcg gcgatggatg ccgccgaagg cgcgcgccag cacatcaccc gctggttcta
     3301 cgactgcccg cacgccttcc acacgaacct ggcggacatg tatgtcgcgg acccccggtt
     3361 caccaggacg tacgaggacc tggcccccgg gctggcgcag tacgtccgtg acgccatcta
     3421 cgccaacgcg gcccgtcacg gcgcatcggg accacccccc ggcgcatagg gaccacagcc
     3481 cacctcagcg gccgaggacc aggcccttct cgtcggcccc cagggaggtg accgcctgga
     3541 ccaccttggc ctggacggcc cggtagcggg ccttggtggc ggcgggcacc gacgggttgg
     3601 agagcgcggc cccgaggtcg acataccgca tgggggtgcc gatcgacatc gaccccggga
     3661 tgtactcggc cttgctcacc gcgaacccgc ccgccgcccg ttcggtgaag gtgaagcgcg
     3721 cggcaacctc ggcccgggtc ggctcgttgc cgctgtgtgc ctggttgtgg atcaggttgc
     3781 ccaggccata ggcgacccac ttgccgttga cccggtcgaa gggctgcacg acgtggacgt
     3841 ggtggccgta gacgaggtcc acatccggcg aggcggtcag cgcggcgacg accgcgcgct
     3901 gctgagcgct cggagcctgg acgtactcgt tcccggcatg cagggcgacg aggacgacgt
     3961 cggcgccggc gcgccgggcg gccttggcgt cggcgagtat cgcggggacg gagatgaggt
     4021 tgacggcgta gcgccgcccc gcgggttcgc tcaagccgtt gagcccgtag gcgtagttga
     4081 gcagggcgac cttgacgccg ttggcgtcga gcagcagcgg cgtgtcggcc tcgcgctggg
     4141 tgcggaaggt ccccgtgtgc ttgaggccgg cgctgtccag cgcgtcgacg gtgcggacga
     4201 gcccggtcca cccggcgtcg acggagtggt tcgacgcggt cgtgcacgcg tcatacccgg
     4261 tgtccgtcag ggctggcagg atgtcctggg ggacctggaa cgtggggtac ccggagtagg
     4321 ggccccccgg tttggccagc ggggtctcga gctggcagat cgcgaggtcg gcgccctcga
     4381 cgacgcggcg cgaggcggcc agcagcgggc ggaagtcgag cccgtgcgtg gccctggcgc
     4441 cgggcagccc ggcatcgcgg cgggcctggc tgacgacagg cccgtggagc aggacgtcgc
     4501 cggtcatgac gacggtgaca ctgcgggcag gcgcacgggt cggcgccggg gtggtgctgg
     4561 ccggcgacga ggtggtcgct gaggggctgg gcgacgtcgg ggccgtggtg gtc
//
LOCUS       DATAIX010000113         1724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_271049, whole genome shotgun sequence.
ACCESSION   DATAIX010000113 DATAIX010000000
VERSION     DATAIX010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271049"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="VHM65_01750"
     CDS             complement(<1..1346)
                     /locus_tag="VHM65_01750"
                     /inference="COORDINATES: protein motif:HMM:NF022650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccE"
                     /protein_id="HEX2704453.1"
                     /translation="MVGPLPLASVVGLEIAALAALLARSAQRPVAGVLLALVGVAGLV
                     LALPVRGAPLGTAVVRRLAFLSRSRVAAAGPGDVVAPSASSLGAEVPGLPVGLSTLFP
                     GAHLRPVRDQAGRVFALAEWATTVGAVVRVGPVAGPLVHARTSTRVPLAATFDRSVGL
                     GIPLDSITLLSLSSAASSQSAGPASPTTGRQEVFLVVRLRPLDARSAIAARGGGQDGV
                     RGALAALTSAVVAQVRSSGLAAEVLDVESLAALADASVGSTTAATAVAAGDLTTWAES
                     WDDVTGPSASHRTFAAVEWNPAAEVADWSGPPGFARAVATEVSPAAGQPRVRVLTRVS
                     DPTVRGADVAGQTLRRHAGHAGMTVRPVSGEQLSGLRATTPFGQGGGARRAAERRPSF
                     GPGGAAPDLACRSRPAQPGRRRRAASRRRSRGNVAGARPRRPAPANDCRSRAGLAGP"
BASE COUNT          253 a          653 c          646 g          172 t
ORIGIN      
        1 gggccagcca gcccggcccg agagcgacaa tcgttcgcgg gcgctggccg acgaggtcga
       61 gcaccagcga cgttccctcg ggaccgacgc cgagatgcag cccgccgtcg acgaccgggc
      121 tgagccggtc gagatcggca cgcgagatcg ggagccgcgc ctccaggtcc gaacgaaggg
      181 cggcgctcag ccgctcgtcg tgccccgccc ccctgcccga agggggtcgt cgcccgaagt
      241 ccgctcagct gctcacccga gaccgggcgg accgtcatac cggcgtggcc ggcgtggcgg
      301 cgcagggtct ggccggcgac atcggcgccc cgcaccgtgg ggtcgctgac ccgggtcagg
      361 acccgcaccc gaggctgacc cgccgctggt gacacctcag tggccacggc gcgagcgaaa
      421 ccgggcggcc ccgaccagtc ggcgacctcg gccgcggggt tccactcgac tgcggcgaac
      481 gtccgatggc tcgccgaggg cccagtcacg tcgtcccagg actcggccca cgtcgtcagg
      541 tctcccgccg cgactgcggt ggcggccgtc gtcgagccga cgctcgcgtc ggcgagcgcg
      601 gcgagcgact cgacatcgag cacctccgcc gccagcccgc tcgagcggac ctgagccacc
      661 actgccgacg tcagagcggc aagtgctccg cggacgccgt cctggccgcc accgcgggcc
      721 gcaatggccg accgggcgtc caacggccgg aggcgcacca cgaggaagac ctcctggcgg
      781 ccggtcgtcg gcgatgccgg gccggcactc tgggacgatg ccgcggagga cagagacagc
      841 agcgtgatcg agtccagcgg tatgccgaga cccaccgacc gatcgaaggt cgccgccagc
      901 gggacccggg tcgaggtccg ggcgtggacc agcgggccgg caacgggccc gacccggacg
      961 accgcgccga ccgtggtcgc ccattcggcc agcgcgaaga cacgaccggc ctggtcgcgc
     1021 acgggtcgga ggtgcgcccc ggggaagagc gtcgacaggc ccacggggag ccccggcacc
     1081 tcagcgccga gggacgaagc actaggggcg acgacgtcgc cggggcccgc cgcagcgacc
     1141 ctcgagcggc tcaggaacgc cagccgacgc acgacggcgg tccccagggg ggcgccacga
     1201 accgggagag ccaggaccag accggccacc ccgaccaggg cgaggagcac cccggcaacc
     1261 ggacgttgcg ccgatcgtgc gagcagggcc gccagagccg cgatctcgag gccgaccacg
     1321 ctcgcgaggg ggagggggcc gaccatgtgc cggaccggat cgcgccggat cacattgcgg
     1381 ctccgtccct gggccggcac ccgggtcggc agccgacccg gaggcggagc ggacgcctga
     1441 gctggcgccc ggctcgatga ctgtgccggg gcgccagccc gacgggggcc tgcggttgga
     1501 ccagggccag tgcgggcgcc cgccccgctg cgtcgagtgg gttcaccgga tcgagcccgg
     1561 gaccggcggg ggccctggtg ctcgccgcgt tcagggttga tgctcaccat gtctgcccct
     1621 ccccctacct gcgcctcgca gcggggcacg cctgctcgtc ggtcgtccga agtctagcga
     1681 ggaccgctcg ggagcccggg ccccccgagc ggtcctcgct agac
//
LOCUS       DATAIX010000114         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_122084, whole genome shotgun sequence.
ACCESSION   DATAIX010000114 DATAIX010000000
VERSION     DATAIX010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122084"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /gene="sigE"
                     /locus_tag="VHM65_01755"
     CDS             complement(<1..656)
                     /gene="sigE"
                     /locus_tag="VHM65_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014154239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigE"
                     /protein_id="HEX2704454.1"
                     /translation="MSDTLTAPAVHAEAGSSASAPGSAWAPPSWEEIVALHSGRVYRL
                     AYRLTGNQHDAEDLTHDVFVRVFRSLHSYTPGTFEGWLHRITTNVFLDKMRRKQRIRF
                     DFLPEDAAGRLASAEPGPEQRYADTHLDDDIQRALDALSPDFRAAVVLCDIEGLSYEE
                     IAATLGVKLGTVRSRIHRGRAQLREALAHRAPTAPAASSPAPAGPRRIPRRGMGMSVR
                     T"
     gene            789..1421
                     /locus_tag="VHM65_01760"
     CDS             789..1421
                     /locus_tag="VHM65_01760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX2704455.1"
                     /translation="MSAQKPASWAYAEEFVPEPAMTDQARRQGEQLGAMPVGSAVGAT
                     LRMLASAMKATSVVEIGTGAGVSGLWLLEGMPGEGVLTTIDVSTEHQRAARAAFASAG
                     VVHQRTRVIAGRALDVLPRMTDGAYDLVFIDGDKLEYPAYVEQAVRLLRSGGVMVLDN
                     MLWGDHVADPAARDEQTRVLRDLGKAIRDDEGFLTVLLPVGDGLLAAVKR"
     gene            complement(1369..>1620)
                     /locus_tag="VHM65_01765"
     CDS             complement(1369..>1620)
                     /locus_tag="VHM65_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704456.1"
                     /translation="VRAGAVVAALFLREFAGSRHWAHLDIAGPGRAEADSGLTVTGPT
                     GFGARLLLDYLAGLDPAAKRPPLTASPRRAGRRRREAAR"
BASE COUNT          241 a          549 c          613 g          217 t
ORIGIN      
        1 gtcctcacgg acatgcccat gccgcgtcgc gggatccggc gcgggcctgc aggagcgggc
       61 gacgaagcag caggggcggt cggcgcgcgg tgggccagcg cctcgcgcag ctgagcccgg
      121 ccccggtgga tgcgggagcg caccgtgccg agcttgaccc cgagcgtcgc agcgatctcc
      181 tcgtaggaga gcccctcgat gtcgcacagg acgaccgcgg cccggaagtc cggcgagagc
      241 gcgtcaaggg cacgctggat gtcgtcatcg agatgggtgt cggcgtaacg ctgttcgggg
      301 ccgggctcgg ccgacgccag ccgcccggca gcatcctcgg gcaggaagtc gaaccggatg
      361 cgctgcttgc ggcgcatctt gtcgaggaac acgttggtcg tgatgcggtg cagccagccc
      421 tcgaacgtac ccggtgtgta ggagtgcaac gagcggaaca cccggacgaa gacgtcgtgg
      481 gtgaggtcct cggcgtcgtg ctggttgccg gtgaggcggt acgccaggcg gtagacgcgc
      541 ccggagtgca gggcgacgat ctcttcccag ctcggcgggg cccaggcgct gccgggggca
      601 gacgcactcg acccagcctc ggcatggacc gcgggggcgg tgagggtgtc ggacacgacg
      661 tgcgcctttc acgagttcag accggtatga cgaaccgggg tgccttcagc gttcccgacg
      721 actctgggag aagcctgaga ggcacctggc gagatcccca gggtccgcgt cagcactagg
      781 gtgaggtcat gagcgcgcag aagccggcca gctgggccta tgccgaggag ttcgtccccg
      841 aacccgcgat gaccgaccag gcgcggcgcc agggtgagca gctcggcgcc atgccggtcg
      901 gcagtgccgt cggcgcgacg cttcggatgc tcgcctcggc gatgaaggcc acgtccgtcg
      961 tcgagatcgg caccggggcc ggcgtgtccg gtctatggct cctcgagggc atgcccggcg
     1021 agggggtcct cacgacgatc gacgtgtcga ccgagcacca gcgtgccgcc agggccgcgt
     1081 tcgcctctgc cggagtggtc caccagcgca cccgggtcat cgccggccgg gcgctcgacg
     1141 tcctgccgcg gatgaccgac ggcgcctacg acctcgtgtt catcgacggc gacaagctgg
     1201 agtaccccgc gtatgtcgag caggcagtcc gcctcctgcg cagcggtggg gtcatggtcc
     1261 tggacaacat gctctggggc gaccacgtcg ccgacccggc cgcccgggac gagcagacgc
     1321 gggtgctgcg tgacctcggc aaggcgatcc gcgacgacga gggcttcctc accgtgctgc
     1381 ttcccgtcgg cgacggcctg ctcgccgcgg tgaagcggtg agcggcggac gcttcgcggc
     1441 cgggtcgaga ccggcgaggt agtccagcag cagccgggca ccgaaccccg tcgggccggt
     1501 cacggtgagc ccgctgtccg cctcggcgcg gccggggccg gcgatgtcca ggtgagccca
     1561 gtgccgggac ccggcgaact cgcgcaggaa caacgccgcg accacggcgc cggcccgcac
//
LOCUS       DATAIX010000115         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_66252, whole genome shotgun sequence.
ACCESSION   DATAIX010000115 DATAIX010000000
VERSION     DATAIX010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66252"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VHM65_01770"
     CDS             <1..912
                     /locus_tag="VHM65_01770"
                     /inference="COORDINATES: protein motif:HMM:NF016114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HEX2704457.1"
                     /translation="GISRFKVARLLDSAVAAGLVRIRIGLPGALDITLSVELQARFGL
                     RHCVVIDSHESAPAEMRRVLAGAAARLLTEIVTPEDVLGLAWARSAADMVGQLRGLAP
                     VPVVQLTGALSSPDLGDTSIELVREVAHRTGGPAYYFYAPLIVPDTATSRALRKQPEI
                     ARAFAQVGAVTKAVVGLGAWAPGQSTLFDAMGAFEREELVRLGVIGEICSSFIDREGH
                     AIVSSISERMIGIDAAGLASVPEVIAIPYGDAKASAVRAAVNGGFVNGLVTHSSLARI
                     LLGDDDLGDDDLGDNDLGDNDLGDHDR"
     gene            924..1619
                     /gene="nucS"
                     /locus_tag="VHM65_01775"
     CDS             924..1619
                     /gene="nucS"
                     /locus_tag="VHM65_01775"
                     /inference="COORDINATES: protein motif:HMM:NF002876.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HEX2704458.1"
                     /translation="MRLVIARCSVDYEGRLQAHLPLATRLLVVKADGSVLVHSDGGSY
                     KPLNWMSPPCVLAEVGPDEAELADGVTGVWVVAHQKSDDRLRVRLHEVLHDSAHDLGV
                     DPGLVKDGVEAHLQRLLAEHIATLGPGYTLVRREYMTAIGPVDILARDPSGGTVAVEI
                     KRRGEIDGVEQLTRYLELMNRDPHLAPVSGVFAAQEIKPQARTLAEDRGIRCLILDLA
                     ALKGIDNVESRLF"
     gene            complement(1668..>2031)
                     /locus_tag="VHM65_01780"
     CDS             complement(1668..>2031)
                     /locus_tag="VHM65_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX2704459.1"
                     /translation="VELASGALEGRPVDDHERVSCEDRWRSGDLGAAWPGGESGTAIL
                     ARMRQALDEVADRHRGEAVLVVSHGGAMSLALPRLVSGAASPTLAVPAVANRAVVALE
                     GDADGWRLTRPWPGSELG"
BASE COUNT          280 a          744 c          698 g          309 t
ORIGIN      
        1 ggcatcagcc ggttcaaggt ggctcggctg ctcgactccg cggtcgcggc ggggttggtg
       61 cggatccgga tcggtctgcc cggcgcgctc gacatcaccc tgtccgtcga gctccaggcg
      121 cgcttcggcc tgcgtcactg cgtcgtcatc gactcccatg agagcgcacc tgccgagatg
      181 cgccgcgtgc tcgccggtgc ggcggcgcgc ctgctgacgg agatcgtcac ccccgaggac
      241 gtgctcgggc tggcctgggc ccggtcggcg gccgacatgg tcggtcagct ccgcgggctc
      301 gcgcccgtcc cggtcgtgca gctgaccggg gcgttgtcga gccccgacct gggcgacacc
      361 tcgatcgagc tcgtccgcga ggtggcacat cgcaccggcg gcccggcgta ctacttctac
      421 gcgccgctca tcgtgcctga cacggcaacc tctcgagctt tgcgcaagca gcctgagatc
      481 gcccgggcgt tcgcccaggt cggagccgtg accaaggccg tcgtcgggct gggcgcctgg
      541 gctcccggcc aaagcacgct gttcgacgcg atgggggcct tcgagcgcga ggagctggtg
      601 cgactcggtg tcatcggcga gatctgcagc tcgttcatcg accgcgaggg ccacgccatc
      661 gtctcctcga tcagtgagcg gatgatcggt atcgacgccg ccgggctggc ttccgtgccg
      721 gaggtcatcg ccataccgta tggcgacgcg aaggcgtcgg ccgtcagggc cgccgtcaac
      781 gggggcttcg tcaacggtct ggtgacccat tcgagcctgg ccaggatcct gctcggtgac
      841 gatgacctcg gtgacgatga cctcggtgac aatgacctcg gtgacaatga cctcggtgac
      901 cacgaccgct aggctcgcct ctcgtgcgac ttgtcattgc ccggtgcagc gtcgactacg
      961 aggggcgcct gcaggcccac ctgcccctgg ccacccggct gctcgtcgtc aaggccgacg
     1021 gctcggtgct ggtccacagc gacgggggct cctacaagcc gctcaactgg atgtccccgc
     1081 cctgcgtgct ggccgaggtg gggcccgacg aggccgagct cgcggacggt gtcaccgggg
     1141 tctgggtggt tgcccaccag aagtccgacg accggctccg ggtgcgcctg cacgaggtgt
     1201 tgcacgactc ggcgcatgac ctcggggtgg accccggtct cgtcaaggac ggcgtcgagg
     1261 cccatctgca gcggctcctc gccgagcaca tcgccacgct ggggccgggc tacaccctcg
     1321 tgcgccggga gtacatgacg gccatcggcc cggtcgacat actcgcccgc gacccgtccg
     1381 ggggcacggt cgcggtcgag atcaagcggc gcggtgagat cgacggggtc gagcagctga
     1441 cccgctacct cgagctgatg aaccgtgacc cccaccttgc cccggtcagc ggtgtcttcg
     1501 cggcccagga gatcaagccc caggcgcgca ctctcgccga ggaccgcggg atccgttgcc
     1561 tcatcctcga cctcgccgcg ctcaagggca tcgacaacgt cgagtcgcgc ctgttctgac
     1621 tccgcccgcc gggatcaccc cagctcagac cccggccacg gccgggatca ccccagctca
     1681 gaccccggcc acggccgggt gagccgccag ccgtccgcat caccttcgag ggctacgacg
     1741 gcacggtttg cgaccgcagg gacagccagc gtgggcgacg cggcaccgct gaccaggcgc
     1801 ggcagcgcaa gggacatcgc cccgccatgg ctgacgacga ggaccgcctc cccgcggtgc
     1861 cggtccgcga cctcgtccag cgcctggcgc atgcgggcga ggatcgccgt tccgctctcg
     1921 ccaccgggcc aggccgcgcc gaggtcgccg gaccgccagc ggtcctcgca gctgacccgt
     1981 tcgtggtcat ccaccgggcg tccctcgagc gctccggagg cgagctccac g
//
LOCUS       DATAIX010000116         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_66290, whole genome shotgun sequence.
ACCESSION   DATAIX010000116 DATAIX010000000
VERSION     DATAIX010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66290"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VHM65_01785"
     CDS             complement(<1..327)
                     /locus_tag="VHM65_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704460.1"
                     /translation="MRWATYTSGSKGEGSRVGLVDGDELRGLDASYTLLGLLEAGGDA
                     LAVAAERARDHPAEVVRLDAVRLLPPVPRPPSVRDFMAFESHVVTAMGALGREVDPVW
                     YEQPVFY"
     gene            complement(352..618)
                     /locus_tag="VHM65_01790"
     CDS             complement(352..618)
                     /locus_tag="VHM65_01790"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX2704461.1"
                     /translation="MTYEVALASSAQRDLQGIPPRYASAILEFIYSVLPDNPRRVGKP
                     LGRDLEGIHSARRGDYRVLYEVHEHTNEVVVIRVSHRASAYRKR"
     gene            complement(615..893)
                     /locus_tag="VHM65_01795"
     CDS             complement(615..893)
                     /locus_tag="VHM65_01795"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX2704462.1"
                     /translation="MQTLPISKAKDRLNELVDEAAATHEHFTITRNGTPAAMLIGMQE
                     WESIQETLFWLSQEGVRQDIERARQEHAAGQTLSEEQVRAQLGIPRRP"
     gene            complement(982..1920)
                     /locus_tag="VHM65_01800"
     CDS             complement(982..1920)
                     /locus_tag="VHM65_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2704463.1"
                     /translation="MSTNGTDHAYAPPGRAVEVSDGVYAYVQPDGSWWINNTGFFVGR
                     RGVVSVDACATETRTRAYLTAIRAVTDAPVTTVLNTHHHGDHTFGNYLFPGATIVGHD
                     SVRAALLAWGLPRAAPFWTDVDWGAVELAPPFLTYSEGVTVYVDDLRCEVRYVGTPAH
                     TTNDSFVWVPERRLLFSGDLLFNGGTPFLVQGSIAGALVALDVLRGLGAETIVPGHGP
                     VCGPEVIERVASYLRFVQDTARRGRAAGLSPLEVARESSLGQFARWLDPERIVGNLHR
                     AYAEDAGAAPGAVIDALAALTDMITFNGGQPLTCHA"
     gene            complement(1953..>2184)
                     /locus_tag="VHM65_01805"
     CDS             complement(1953..>2184)
                     /locus_tag="VHM65_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704464.1"
                     /translation="VLGAETVALPHDPGALAYRVADSTVLALAERQRVLAAPDTVARL
                     RLVGYLLRRERSLVEQFRAVPQSPDFGGAGLN"
BASE COUNT          332 a          746 c          784 g          322 t
ORIGIN      
        1 gtagaagacg ggctgctcgt accagaccgg gtcgacctcg cggccgagcg cccccatcgc
       61 ggtgacgacg tggctctcga acgccatgaa gtcacgcacc gacggcggcc gcggcaccgg
      121 cggcagcagc cgcacggcat cgaggcgaac cacctcggcc gggtggtcgc gggcccgctc
      181 cgcggcgacc gcgagcgcgt cgcctcctgc ttcgagcaga cccagcaggg tgtatgacgc
      241 gtccagcccc cgcagctcgt cgccgtcgac gagcccgacc cggctgccct cgccctttga
      301 acccgatgtg tatgtcgccc aacgcatgtg cggctcctcc cactgaggtg tctagcgctt
      361 ccggtacgcg ctagcgcgat ggctgacccg gattacgacg acttcgttgg tgtgctcgtg
      421 gacctcgtac aggactcggt agtcaccgcg gcgagccgag tggatcccct cgaggtcacg
      481 gcccaggggc ttgccgaccc gccgtgggtt gtcgggaaga accgagtaga tgaactcgag
      541 gatggctgag gcatagcgag gggggatgcc ctgaagatca cgctgtgcgc tgctggcaag
      601 ggcgacctca taggtcatgg acgacgcggg atgccgagct gagcgcgcac ctgctcttca
      661 ctcagtgtct gaccagcggc gtgttcctgg cgggcgcgct cgatgtcctg gcggacgccc
      721 tcctgagaca gccagaagag ggtctcctgg atggactccc actcctgcat gccgatcagc
      781 atcgctgccg gagtgccgtt ccgggtgatg gtgaagtgct cgtgggtggc ggcggcctcg
      841 tcgacgagct cgttgaggcg gtccttggcc ttggagatcg gcagggtctg cataagtcga
      901 tcgtacgcct gaatatcggc cgtgaacgtg cccggactca ggccgctatg gggcgacggg
      961 tcgccgaccg atgcggctcg ctcaggcgtg gcaggtcagc ggctggccgc cgttgaaggt
     1021 gatcatgtcg gtgagcgcgg ccagcgcgtc gatcaccgcg cccggcgcag cgccggcgtc
     1081 ctcggcatac gcgcggtgca ggttgccgac gatccgctcg gggtcgagcc atctggcgaa
     1141 ctgcccgagg ctgctctccc gagcgacctc caacggcgag agccccgccg cgcgcccccg
     1201 ccgcgcggtg tcctggacga agcgcaggta tgacgcgacc cgctcgatca cctcgggccc
     1261 gcacaccggc ccgtgaccgg gcacgatcgt ctcggcgccg aggccgcgga gcacgtcgag
     1321 agccacgagc gctccggcga tcgagccctg gacgaggaag ggcgtcccgc cgttgaagag
     1381 caggtccccg ctgaagagca gccgccgctc cgggacccag acgaacgagt cgttcgtcgt
     1441 atgggcgggc gtgccgacat accggacctc gcacctcagg tcgtcgacat agaccgtcac
     1501 cccctcggaa taggtgagga agggcggggc gagctcgacc gcgccccagt cgacatcggt
     1561 ccagaacggc gccgcccggg gcagccccca cgcgagcagc gcggcccgga cgctgtcgtg
     1621 cccgacgatc gtggcaccgg ggaagaggta gttgccgaac gtgtggtccc cgtggtggtg
     1681 cgtgttgagc accgtcgtga ccggcgcgtc ggtgaccgcc cggatcgccg tgagatacgc
     1741 gcgcgtccgg gtctcggtgg cgcaggcgtc gacactgaca accccccggc gcccgacgaa
     1801 gaagcccgtg ttgttgatcc accagctgcc gtcgggctgg acataggcgt agaccccgtc
     1861 gctcacctcg acggcccggc ccggcggggc gtaagcgtgg tcggtgccgt tcgtgctcat
     1921 gcccgattgt ggcccttcgc cgccgggttg gctcagttga ggcccgcgcc gccgaagtcg
     1981 gggctctggg gcacggcgcg gaactgctcg acgagcgagc gctcccggcg caggaggtag
     2041 ccgacgagtc gcagccgcgc gaccgtgtcc ggggcggcca gaacgcgctg ccgctcagcc
     2101 agggcgagca ccgtcgagtc ggccacccgg taggcgagcg cgcccggatc atgtggtaag
     2161 gcaacggttt cggcaccaag gacc
//
LOCUS       DATAIX010000117         1752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_241288, whole genome shotgun sequence.
ACCESSION   DATAIX010000117 DATAIX010000000
VERSION     DATAIX010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241288"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..435)
                     /locus_tag="VHM65_01810"
     CDS             complement(34..435)
                     /locus_tag="VHM65_01810"
                     /inference="COORDINATES: protein motif:HMM:NF023453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3515 family protein"
                     /protein_id="HEX2704465.1"
                     /translation="MFAACTRGVEVTPPSGAAGAACRAAAARWPTKVGRAERVTTTPE
                     SPGTAAWGDPALIARCGLAPLTPTTEDCLGVDGVDWVVRRLRDGASFTTYGTDPALEV
                     LIPAAYVPEPTLLPAFGAAARALPQNGRHCS"
     gene            complement(485..721)
                     /locus_tag="VHM65_01815"
     CDS             complement(485..721)
                     /locus_tag="VHM65_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX2704466.1"
                     /translation="MVVQAYILIQTEVGKATTVAEAIARISGVAMAEDVTGPYDVIAR
                     VEAGSVDELGKLVIAKIQDVPGITRTLTCTVVHV"
     gene            820..>1752
                     /gene="thiL"
                     /locus_tag="VHM65_01820"
     CDS             820..>1752
                     /gene="thiL"
                     /locus_tag="VHM65_01820"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX2704467.1"
                     /translation="MVSTPSPDRAAGPGRAERLADLSEEAVLARILPLMHATAAAAVW
                     VGPGDDAALLRVSSGAVVATTDTMVRDRDWRDRFSTGADVGHKTVAQNLADVAAMGAV
                     PTGLLVTLVADPQTPMSWVEDFADGLGSAARAARVPVIGGDLSSAPAGVLTVCVTALG
                     ELATGHAVLRSGASPGDVVALCGSLGLAHAGWRLLEQGEPDAMPVAVARQRRPEAPID
                     EGPVAARAGATSMIDISDGLLRDAARVAQASGARLDLSREALSGDVARLRPAMGERLA
                     WECVLAGGEEHSLLATFPCAEQVPAAWRVIGSVQP"
BASE COUNT          221 a          610 c          671 g          250 t
ORIGIN      
        1 ggtgggtgga atgggctgcc cgctgaggct ggtctagctg cagtgccgcc cgttctgggg
       61 cagcgcgcgg gcggccgccc cgaaggcggg cagcaaggtc ggttcgggca cgtaggcggc
      121 ggggatcagc acctcgagcg ccggatcggt gccatatgtc gtgaagcttg cgccgtcgcg
      181 cagccgccgg accacccagt cgacgccgtc cacgccgagg cagtcctcgg tggtcggggt
      241 cagcggcgcg aggccgcatc gggcgatgag ggccgggtcg ccccaagccg ctgttcccgg
      301 ggactcgggg gtagtcgtga cgcgttcggc gcgaccgacc ttggtcggcc atcgggcggc
      361 cgcggcccgg caggcagcac cggcggcccc gctgggcggg gtgacctcga cgccgcgggt
      421 gcacgcggca aacatcagca gggccgtgcc cgcgcgcgtc gcgtggacgg ctcggacacg
      481 gatcctagac gtggacgacg gtgcaggtga gcgtgcgggt gatgccgggg acgtcctgga
      541 tcttggcgat gacgagcttg cccagctcgt cgacgctgcc cgcctcgacc cgggcgatga
      601 cgtcatacgg accggtgacg tcctcggcca tcgccacgcc actgatcctg gcaatggcct
      661 cggcaacagt cgtcgcctta ccgacctccg tctgaatcag gatgtaggcc tgaaccacca
      721 cgacacacct cacttcagtt gggttgaccg gggctctgcc cgacatggtc ctgggcgggt
      781 ccgcctgaac cgcacgagac agacgctacc gtgcgggtga tggtttcgac acccagcccc
      841 gaccgggcgg ccgggcccgg gcgggccgag cgcctcgctg acctcagtga ggaggccgtc
      901 ctcgcccgga tcctgccgtt gatgcacgcg actgccgcag cggccgtgtg ggtcgggccc
      961 ggtgacgacg ccgccctgct ccgggtgtcc tcgggcgcgg tggtggcgac gactgacacg
     1021 atggtgcgcg accgggactg gcgggaccgc ttctcgaccg gcgccgacgt gggccacaag
     1081 acggtcgcgc agaacctcgc cgacgttgct gcgatgggcg ccgtgcccac cgggctgctc
     1141 gtcaccctgg tcgccgaccc gcagacaccc atgagctggg tcgaggactt cgccgacggg
     1201 ctgggctcgg ccgctcgcgc ggcacgggtg cccgtcatcg gaggggacct gtcgtcggcg
     1261 ccggccggtg tgctcaccgt gtgcgtgacc gccctgggcg agctcgcgac cgggcacgcc
     1321 gtgctgcgca gcggcgcgag cccgggggat gtggtcgcgc tgtgcgggtc gctcggactg
     1381 gcgcacgccg gctggcgact gctcgagcag ggggagcccg acgcgatgcc cgtcgccgtc
     1441 gcccgtcagc gtcgtcccga ggcgccgatc gatgagggac ccgtggcggc acgagccggt
     1501 gccacctcga tgatcgacat ctccgatggg ctgcttcggg acgccgcgcg ggtggctcag
     1561 gccagcggag cgcgcctcga cctgtcccgg gaggccctct ccggcgatgt ggcccggctg
     1621 aggccagcca tgggggagcg cctggcctgg gagtgtgtcc tggccggcgg cgaagaacac
     1681 agcctgctgg cgaccttccc ctgcgccgag caggtccccg ccgcctggcg ggtcatcggg
     1741 tcggtacagc ct
//
LOCUS       DATAIX010000118         7395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_66371, whole genome shotgun sequence.
ACCESSION   DATAIX010000118 DATAIX010000000
VERSION     DATAIX010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66371"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VHM65_01825"
     CDS             <1..604
                     /locus_tag="VHM65_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX2704468.1"
                     /translation="AVLEATIDQLGAGARWLVGCGSLPPGMDDSFYAELVRRARYAGA
                     RVAIDTSGSPLTVAVTAWPDLIKPNLEELEDLVGETLSTLGDVRDAAATLAESGIGTV
                     VVSLGRHGALLVSATQVAHAQATVAKPLSTVGAGDSLLAGFLHAISRGTAAPEALANG
                     AAWGGAAVSLPGSRMPTPGEVAAIPVSLTTTPDFTLHVTD"
     gene            654..1100
                     /locus_tag="VHM65_01830"
     CDS             654..1100
                     /locus_tag="VHM65_01830"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HEX2704469.1"
                     /translation="MSLITEAQVVLDLDAPDRHVATRTLASTLVADGRCTDLDLFLED
                     VRKREEQMATGLPGGIGIPHARSAAITTPSLVFGRSAGIDWGAEDGPATLIFLIAAPD
                     GGGQEHLQILAKLARKLMRVDFRQALHDAPDATTVVDIVNREVVNA"
     gene            1097..2641
                     /locus_tag="VHM65_01835"
     CDS             1097..2641
                     /locus_tag="VHM65_01835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HEX2704470.1"
                     /translation="MKLVGVTSCPTGIAHTYMAAEALEQAAKAAGHEMIVETQGSAGS
                     DPIPQAVINAADAVVFAHDLEVVDRARFAGKPTIDVGVKKAISDGPGLIAQAVAAAEA
                     FAANPQDAAARVSAAAPAGGGGLTSKVDSGASTATKIRQWLMTGVSYMIPFVAAGGIL
                     IAVSFMLAQVAGGEKGAIDVTKLYTLNPAVPTKLTILQNAFNPGNGMHWAALLFLIGA
                     AAFGFLVPILSGFIAFGIADRPGIVPGIVGGFIAASMGAGFLGGIATGFLGGFAARWV
                     SRWKVAKGVRGVMPVVVIPLLSTLITGGLFIVVLGRPIKALMDALTRGLNGMTGSSAI
                     ILGLILGAMMGFDLGGPVNKVAYTFATTGLAAAGTATDAPQLKIMAAVMAAGMVAPLA
                     MALATTLRPQLFTEPERENGKAAWLLGASFISEGAIPFAAADPVRIVVSSVIGSSITG
                     ALAMGFGTTLRAPHGGIWVLPLIGGFLLFLVALAVGVVVTAGIVVALKQRDHSQAAQA
                     LRAAAV"
     gene            2701..2970
                     /locus_tag="VHM65_01840"
     CDS             2701..2970
                     /locus_tag="VHM65_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX2704471.1"
                     /translation="MASRTVTIASSVGLHARPASLFVQAASATGLDVEIGRPGEEAVD
                     ATSILGVMALGAAHGEEVVLTAEGDGADQALDSLVALLSRDLDAE"
     gene            2978..4663
                     /gene="ptsP"
                     /locus_tag="VHM65_01845"
     CDS             2978..4663
                     /gene="ptsP"
                     /locus_tag="VHM65_01845"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX2704472.1"
                     /translation="MATQPQVMRGIGVSPGTAVGPVIQVRPPARPPANEPATTDAAAT
                     HVSIRGIFEAVAVSLEERAARAEDTAQQILTAGALMARDKGLQKAIGKHLATGAGPST
                     AVNDAVEEYCAKFEALGGYFAERVADLRDVRDRAIARILGVPEPGVPTLTGPSVIVAY
                     DLAPAETATLDPAMVVGIVTEAGGRTSHTAILAAQMGIPAVVQVEGALGIRFGTTVAV
                     DGDAGEVTLEPDREYQTRLAAKRDRRSAALAASHGPGVTKDGHAVALLANIGGVDDAV
                     AAGAQDVEGVGLFRTEFVFLGNDTAPTLAEQTEIYRKVFEPFGGRRVVVRTLDAGADK
                     PLAFADLGPEENPALGRRGLRLSAERPELLDTQLEALAVAARATGADVRVMAPMVATA
                     EEAAWFARRVRENGLPKVGVMIEVPGAALTAKYVLAEVDFGSLGTNDLAQYTMAADRM
                     QGALSDLLDPWQPAVLHVVAAACEGGRVTGRPIGVCGESAGDPLLALVLTGLGVSSLS
                     MAPSKVALVRHALSLHSLPACTRMASLALDARTASDARAAVLETAAPALTELL"
     gene            4707..5423
                     /locus_tag="VHM65_01850"
     CDS             4707..5423
                     /locus_tag="VHM65_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2704473.1"
                     /translation="MATAPHDSPPDVPVEAVIFDVHSTLMDQGDPTRWLAVALARLGR
                     APDPVAELGPPEAARLAVALDQLWEGAREIDPSSSRDVAPADHRRVFDVLTQSQMGIA
                     ADLGGALYETMLDTWQPFEDTVPTLVALRDLGIRIAVLSNVGIDIRGMLERAGVMALV
                     DVAVFSCEVGVVKPDPAIFAHAVEGLGVPAERILMVGDHWADDGAAAALGIRTLILPR
                     TRGPRHGLGLVVAAASSHAY"
     gene            5428..5865
                     /locus_tag="VHM65_01855"
     CDS             5428..5865
                     /locus_tag="VHM65_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704474.1"
                     /translation="MLTFELATALRRTGLAWTPAPGDRFVITTDGLHEDIYHVSEMTV
                     EVHEFVDGVVIGFNGTTEWALDSIDLGDVLWFPREDQLRAALGPAFVRLETVQDGYVV
                     TVASGGRELRALDIDAERAYARALVRVLADATPPPSSTSRSTP"
     gene            complement(6085..>7395)
                     /locus_tag="VHM65_01860"
     CDS             complement(6085..>7395)
                     /locus_tag="VHM65_01860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX2704475.1"
                     /translation="RWPDIARVPRGLRSRFASRIAQTLTRQAVARLAMRMVLPGGEVC
                     GGAARDTAPTIVVHDPEHFFARLADSGLVGFGEAYMAGDWSSPDLAAVLTVMASQMGS
                     LVPMPLQKLRAMYVVRHPDTERNTSDNSRSNISRHYDLSNELFELFLDESLTYSSALF
                     PGVSAEQAGAVPLGAKVEVSRPGAPSRWEDLAAAQRLKIDRMLDAARVGQGTRVLEIG
                     SGWGELAIRAASRGATVRTITLSVEQQGKARSRIARAGFSDRVSVDLVDYRHVEGSYD
                     AVVSCEMVEAVGHEYWPDYFRAIDARLAPGGRAVVQAITMPHDRMLATRRTYTWMHKY
                     IFPGGFLPSTEAIVAVTRRHTSLRVVERLRMGAHYAQTLRLWHERFEAGWPQIAELTS
                     GPLAGFDFDETFRRMWLFYLDYSRAGFSSGYLDVQQIVLERPNR"
BASE COUNT         1053 a         2766 c         2522 g         1054 t
ORIGIN      
        1 cgccgtcctc gaggcgacca tcgaccagct cggtgccggt gcacgctggc tcgtcgggtg
       61 cgggagcctc ccccccggga tggacgactc gttctatgcc gagctcgttc gccgagcgcg
      121 gtatgccggc gcgcgggtcg cgatcgacac ctccgggtcc ccgttgacgg tcgcggtcac
      181 ggcctggccg gatctgatca agcccaacct cgaggagctc gaagacctcg tcggcgagac
      241 cctgtcgacc cttggcgacg tgcgcgacgc ggcagccaca ctcgccgaga gcggcatcgg
      301 caccgttgtc gtgagcctcg gcaggcacgg cgccctgctc gtcagcgcca cgcaggtcgc
      361 ccacgcccag gccacggtgg ccaagccgct ctcgacggtc ggagcgggcg actcgctgct
      421 cgccgggttc ctgcacgcca tctcgcgcgg gaccgccgcg ccggaggctc ttgccaacgg
      481 cgccgcctgg ggcggcgccg ccgtcagcct ccccggtagc cgcatgccca ccccgggtga
      541 ggtggccgcc ataccggtat ccctcaccac cacacccgac ttcaccctcc acgtcacgga
      601 ctgatccagt caccctcccc ctgaaaggaa cacaccatgt caaggagcac accatgtcat
      661 tgatcaccga agcacaggtc gtcctcgacc tcgatgcccc cgaccgccac gtggcgaccc
      721 ggaccctcgc gtcgacgctg gtcgcggacg ggcgctgcac ggacctcgac ctgttcctcg
      781 aggacgtgcg caagcgcgag gagcagatgg ccacgggcct gccgggcggt atcggtatcc
      841 cgcacgcacg cagtgccgcg atcacgaccc cgtcgctcgt cttcgggcgg tcagcgggga
      901 tcgactgggg cgccgaggac ggccccgcca ccctgatctt cctcatcgcc gcccctgacg
      961 gcggaggtca ggaacacctg cagatcctgg ccaagctggc ccgcaagctc atgcgggtgg
     1021 acttccgcca ggccctgcat gacgcacccg acgccacaac ggtcgtcgac atcgtgaacc
     1081 gagaggtcgt gaacgcatga agctcgtcgg agtgacatcg tgccccacgg gcatcgccca
     1141 cacctacatg gccgccgagg ccctggagca ggccgccaag gccgccgggc acgagatgat
     1201 cgtggagacc cagggctcgg cgggctctga cccgatcccc caggccgtca tcaacgccgc
     1261 cgacgccgtg gtcttcgccc acgacctcga ggtcgtcgac cgggcgcgct tcgccgggaa
     1321 gccgaccatc gatgtcggcg tcaagaaggc catctccgac ggtcccggcc tcatcgcaca
     1381 ggccgttgcc gccgcggagg cgttcgcggc caacccccag gacgcggcag cccgggtgtc
     1441 tgccgccgct ccggccgggg gcggcgggct cacgagcaag gtcgactccg gagccagcac
     1501 ggccaccaag atccgccagt ggctcatgac cggcgtgtcc tacatgatcc cgttcgtcgc
     1561 cgcgggcggc atcctcatcg ccgtgtcctt catgctcgcc caggtggcgg gcggggagaa
     1621 gggagccatc gacgtcacca agctgtacac gctcaacccc gccgtcccca cgaagctgac
     1681 catcctgcag aacgcgttca accccggcaa cggcatgcac tgggccgcgc tgctcttcct
     1741 catcggggcg gccgccttcg ggttcctcgt gccgatcctg tccggcttca tcgccttcgg
     1801 catcgccgac cgtccaggca tcgtgcccgg catcgtcggc gggttcatcg ccgcgagcat
     1861 gggcgccggc ttcctcggcg gcatcgccac cggcttcctc ggcgggttcg ccgctcgttg
     1921 ggtgagccgg tggaaggtcg ccaagggcgt ccggggcgtc atgccggtcg tcgtcatccc
     1981 gctgctgtcc acccttatca ccgggggtct gttcatcgtc gtgctcggcc gtccgatcaa
     2041 ggccctcatg gacgccctga cccgtggcct caacgggatg acgggcagca gcgcgatcat
     2101 cctcgggctc atcctcggcg ccatgatggg cttcgacctc ggtggcccgg tcaacaaggt
     2161 cgcctacacc ttcgcgacga ccggtctcgc agcggccggg acggcgacgg acgctcccca
     2221 gctcaagatc atggccgcgg tcatggccgc cgggatggtc gccccgctcg cgatggccct
     2281 ggccacgaca ctccggccgc agctgttcac cgagcccgag cgcgagaacg gcaaggccgc
     2341 gtggctgctc ggggcgtcgt tcatctccga aggcgcgatc ccgttcgccg ccgccgaccc
     2401 ggtccggatc gtcgtgtcct cggtcatcgg ctcgtcgatc accggcgccc tggcgatggg
     2461 ctttggcacc acgctgcgcg cgccgcacgg cggcatctgg gtcctgccgc tcatcggtgg
     2521 gttcctgctc ttcctcgtcg cgctcgccgt cggcgtcgtc gtcacggcgg ggatcgtcgt
     2581 cgccctcaag cagcgcgacc actcccaggc ggcgcaggcc ctgcgcgcag ccgccgtctg
     2641 acccaacccg ctcgagccag ccacccggca tactctcgcc gtgacctaag gagactgacc
     2701 atggcaagcc gtaccgtcac catcgcctcg tccgtggggc tgcacgcccg cccggcctcg
     2761 ctcttcgtcc aggccgcttc ggcgaccggg ctcgacgtcg agatcggccg tcccggagag
     2821 gaggccgtcg acgcgacgag catcctcggg gtgatggccc tcggcgccgc ccacggcgag
     2881 gaggtcgtgc tcaccgccga aggagacggg gccgaccagg cgctcgactc cctcgtcgcg
     2941 ctcctgtccc gcgacctcga cgcggagtga gtggctgatg gccacccagc cgcaggtcat
     3001 gcgcggcatc ggggtcagcc cgggaacggc ggtcggaccg gtgatccagg tccggccgcc
     3061 ggcccggccg ccggccaacg agccggcgac cacagacgcc gcagcgacgc acgtcagcat
     3121 cagggggatc ttcgaagccg tcgccgtcag cctggaggag cgggctgccc gggcggagga
     3181 caccgcccag cagatcctca ccgcgggcgc cctcatggcc cgcgacaagg gcctgcagaa
     3241 ggccatcggc aagcacctcg ccacgggcgc cggcccgtcg acggcggtca acgacgccgt
     3301 cgaggagtac tgcgccaagt tcgaggcgct cggcggctac ttcgccgagc gggtcgccga
     3361 cctgcgtgac gtccgtgacc gggccatcgc gcggatcctc ggcgtccctg agccaggcgt
     3421 gccgaccctg accggcccca gcgtcatcgt ggcgtacgac ctggctccgg ccgagacggc
     3481 gacgctcgac ccggccatgg tcgtcgggat cgtcaccgag gccggcgggc gcacgagcca
     3541 cacggcgatc ctcgctgccc agatggggat ccctgccgtc gtccaggtcg agggcgccct
     3601 cggcatccgc ttcggcacca cggttgccgt cgacggggac gccggtgagg tcaccctcga
     3661 acccgaccgc gagtaccaga cccggctcgc cgccaagcgg gaccgccgct cggccgccct
     3721 ggccgcctcg cacggtcccg gtgtcaccaa ggacggccac gccgtggcgt tactcgccaa
     3781 catcggcggg gtcgacgacg cggtggcggc tggcgcccag gacgtcgagg gcgtcgggct
     3841 cttccgcacc gagttcgtct tcctcggcaa cgacacggcg ccgacgctcg cggaacagac
     3901 ggagatctac cgcaaggtct tcgagccctt cgggggtcgc cgggtcgtcg tccggaccct
     3961 cgatgccggc gccgacaagc ccctggcctt cgccgacctg ggaccggagg agaaccccgc
     4021 cctcgggcgc cggggcctgc gcctctcggc cgagcggccc gagctgctcg acacccagct
     4081 cgaggccctg gccgtcgctg cccgcgccac gggcgccgac gtgcgggtca tggccccgat
     4141 ggtcgccacg gccgaggagg cggcctggtt cgcccggcgg gtccgcgaga acgggctgcc
     4201 caaggtcggg gtcatgatcg aggtgcccgg cgcggcgctg accgccaagt acgtgctcgc
     4261 cgaggtcgac ttcggcagcc tgggcaccaa cgacctcgcg cagtacacga tggccgccga
     4321 ccggatgcag ggtgccctgt cggacctgct cgacccgtgg cagccggccg tcctgcacgt
     4381 cgtggccgcc gcctgcgagg gcgggcgggt caccggccgg ccgatcgggg tctgcggcga
     4441 gtcggccggc gaccccctgc tggcgctcgt gctcaccggg ctcggggtgt cgagcctgtc
     4501 gatggcgccg tcgaaggtgg ctctggtacg ccacgccctg tcgctgcaca gcctgccggc
     4561 ctgcacgcgg atggcgtccc tcgccctgga cgcgcgaacg gcgtccgacg cgcgggctgc
     4621 ggtgctcgag accgcggccc ccgcgctcac cgagctcctc tgacatgggc gatggggtgc
     4681 ggtcggcgtc atacgctcac gaggtgatgg cgaccgcgcc gcacgacagt cccccggacg
     4741 tcccggtcga ggccgtcatc ttcgacgtgc actcgaccct catggaccag ggggacccga
     4801 cccgatggct ggcggtagcg ctcgcccgcc tcggccgcgc tcccgacccg gtcgccgagc
     4861 tcggcccccc cgaggccgcc cgccttgccg tggccctgga ccagctctgg gagggggccc
     4921 gcgagatcga ccccagcagc agccgtgacg tcgctcccgc cgaccaccgc cgggtcttcg
     4981 acgtcctcac ccagagccag atgggcatcg ctgccgacct cggcggcgcc ctctacgaga
     5041 cgatgctcga cacctggcag ccattcgagg acaccgtccc gaccctcgtc gcgctccggg
     5101 acctcgggat tcggatcgcc gtactgtcca acgtcggtat cgacatccgc ggcatgctcg
     5161 aacgggccgg cgtcatggcg ctcgtcgatg tcgcggtgtt ctcctgcgag gtcggcgtcg
     5221 tcaagcccga cccggcaatc ttcgcccacg ccgtcgaggg gctcggggtg cccgccgagc
     5281 gcatcctcat ggtcggtgac cactgggccg acgacggcgc cgcagcagcg ctcggcatcc
     5341 gcacgctgat cctgccgcgg acccgcggtc cccggcatgg gctggggctc gtcgtggccg
     5401 cggcgagcag ccacgcatac tgacgtcatg ctgaccttcg agctggccac ggccctgcgc
     5461 cggaccggac ttgcgtggac gcccgcccct ggcgaccgct tcgtcatcac gaccgacggc
     5521 ctgcacgagg acatctacca cgtgtcggag atgacagtcg aggtgcacga gttcgtcgac
     5581 ggcgtggtca tcggcttcaa cggcacgacc gagtgggcgc tggactcgat cgacctcggt
     5641 gatgtgctgt ggttcccgcg cgaggaccag ctgcgagccg cgctcgggcc ggccttcgtg
     5701 cggctcgaga ccgtccagga cgggtatgtc gtcacagtcg cctcgggtgg acgcgagctg
     5761 cgggctctcg acatcgacgc cgaacgggcc tacgcgcggg cgctcgtgcg cgtcctggcc
     5821 gacgcgacgc cgccgccatc atccacgtcg cgctcgacac catgatcgac actctggcca
     5881 tcctcgcgcc gcatagtggc tccgcgcgcg gatgatatgc gtcgcgagga gtcacaatgc
     5941 ggcgcgcgca tgcctggacc cccgaacagg ccaaacaaag cttcggtgac ccggtttatc
     6001 ccgatttatc cgtatttagc ggccagccgg cgactgtttg acctgttccc cggtacggca
     6061 caacgtccgg tctgcgccgc ccgcctaccg gttggggcgc tcgaggacga tctgctggac
     6121 gtcgaggtag cccgaggaaa agcccgcccg cgagtagtcc aggtagaaga gccacatccg
     6181 gcggaaggtc tcgtcgaagt cgaacccggc cagcggcccg gaggtgagct cggcgatctg
     6241 cggccagccg gcctcgaacc gctcgtgcca gagccgcagg gtctgggcgt agtgggcacc
     6301 catgcggagc cgctcgacga cccgcagcga ggtatgccgc cgggtcaccg cgacgatcgc
     6361 ctcggtcgag ggcaggaacc cgccggggaa gatgtacttg tgcatccagg tgtaggtccg
     6421 ccgggtcgcg agcatccggt cgtggggcat cgtgatggcc tggacgacgg cgcggccgcc
     6481 gggcgccagc cgcgcatcga tcgcgcggaa gtagtcgggc cagtactcgt gcccgaccgc
     6541 ctcgaccatc tcgcacgaca ccaccgcgtc atagctgccc tcgacgtggc gatagtcgac
     6601 gaggtcgacg ctgacccggt cggagaaccc cgcgcgcgcg atgcgggagc gtgccttccc
     6661 ctgctgctcc accgacaggg tgatcgtgcg cactgtcgca ccgcgggagg ctgcccggat
     6721 cgcgagctcg ccccatcccg acccgatctc gagcacccgg gtgccctgcc ccacgcgagc
     6781 cgcgtcgagc atccggtcga tcttgagccg ttgcgccgcg gcgaggtcct cccagcggga
     6841 gggcgcaccc ggccgcgaga cctcgacctt cgcaccgagc ggcaccgcgc cggcctgctc
     6901 ggcgctgaca ccggggaaga gcgccgagga gtaggtcagc gactcgtcga ggaagagctc
     6961 gaagagctcg ttggacaggt cgtagtggcg cgagatgttc gaacggctgt tgtccgaggt
     7021 gttgcgttcg gtgtccggat ggcgtacgac atacatcgcc cggagcttct ggagcggcat
     7081 cggcacgagc gaccccatct ggctcgccat gaccgtgagc acggcggcga ggtcgggcga
     7141 ggaccagtcc ccggccatgt aggcctcccc gaacccgacg agcccgctgt ccgcgagccg
     7201 ggcgaagaaa tgctccgggt cgtggaccac gatggtcggt gccgtgtcgc gggcagcccc
     7261 gccgcacacc tcaccgccgg gcaggaccat ccgcatcgcg agccgcgcga cggcctggcg
     7321 cgtcagcgtc tgcgcgatcc gcgacgcaaa gcggctccgc aggccgcggg ggacacgggc
     7381 aatgtcaggc cagcg
//
LOCUS       DATAIX010000119         1659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_66489, whole genome shotgun sequence.
ACCESSION   DATAIX010000119 DATAIX010000000
VERSION     DATAIX010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66489"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VHM65_01865"
     CDS             complement(<1..318)
                     /locus_tag="VHM65_01865"
                     /inference="COORDINATES: protein motif:HMM:NF021575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEX2704476.1"
                     /translation="MKVLLIQVVLITGVLVLAARVIIGRSARTQAFRRLWLVVLAGLA
                     IWSILFPSVWTRMAGVVGVGRGTDLVLYALVVAFLSFTVTTYLRFRELEARYTTLARR
                     IALD"
     gene            complement(350..1045)
                     /locus_tag="VHM65_01870"
     CDS             complement(350..1045)
                     /locus_tag="VHM65_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2704477.1"
                     /translation="MTSTPNDTVWVVLAVFNEASVVGDVVRGLRVAFPHVVCVDDGSA
                     DDSAGHASRAGAVVVRHPVNLGQGAALQTGFEYALRDPGMRYVVTYDADGQHQVDDVR
                     LMLDRIRAGDVDVVFGSRFLDERTSPSLVRALVLRAAVAYTNLTTHTKLSDAHNGLRV
                     LSRDVVARLQITQNRMAHATELVGQVGAMTLAGRPVRYAEAPVHILYTEYSSEKGQSL
                     WNAINILVDLILH"
     gene            1108..>1659
                     /locus_tag="VHM65_01875"
     CDS             1108..>1659
                     /locus_tag="VHM65_01875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2704478.1"
                     /translation="MASERVHVLFVVTNYPPHHGGVQVHVQALAREIVALGASATVVC
                     LGRNPGRRDEDGVRVVTLQRHLGFAEVLALPGPREWRRRMPDLAREATHISTHTRFFP
                     MSWLGLRAGRRAGRPVVHTEHGAGFVRTGAPVVDLLARLVDLTMGRAVLRGATRVLAV
                     SDAVADFVRHLAGRESVVVGNGVH"
BASE COUNT          253 a          575 c          597 g          234 t
ORIGIN      
        1 gtcgagcgcg atccgccggg ccagcgtcgt gtagcgagcc tcgagctcgc gaaagcgcag
       61 gtaggtcgtc acggtaaagc tgaggaacgc gacgacgagc gcatagagca cgaggtcggt
      121 cccgcgaccg acgccgacga ccccggccat ccgggtccac acactgggga acaggatcga
      181 ccagatcgcc agccccgcga gcacgaccag ccagagccgg cggaaggcct gggtccgggc
      241 actgcgtccg atgatgacgc gcgcggccag gacgaggacg cccgtgatga ggacgacctg
      301 gatcagcagg accttcacgg cgtgccggcc gggcgcccgc tcgccctgct cagtgcagga
      361 tgaggtcgac gaggatgttg atggcgttcc acagtgactg gcccttctcg ctcgagtact
      421 ccgtgtagag gatgtgcacc ggcgcttcgg cataccgcac cgggcgtccg gcgagcgtca
      481 tggcgccgac ctgcccgacg agctcggtgg cgtgggccat ccggttctgg gtgatctgca
      541 gccgggccac gacgtcccgg gacaggaccc gcagcccgtt gtgggcgtcg ctgagcttcg
      601 tgtgcgtcgt gaggttcgtg tacgccaccg cggcccgcag cacgagggcc cgcaccaggg
      661 acggcgacgt gcgctcgtcg aggaaccgtg acccgaacac gacatcgaca tctccggccc
      721 ggatccggtc gagcatgagc cggacgtcgt cgacctggtg ctggccgtcc gcgtcatacg
      781 tcacgacgta cctcatccca ggatcacgca acgcgtactc gaagccggtc tgcagggccg
      841 ccccctgacc gaggttgacc gggtgcctca cgaccacggc gccggcccgg gatgcgtgcc
      901 ccgcagagtc gtcggcggag ccgtcgtcga cacagacgac atgggggaag gcgacccgca
      961 gcccgcgcac gacgtcaccg acaaccgacg cctcgttgaa gacggcgagg acgacccata
     1021 ccgtgtcgtt gggggtagat gtcaccaagg tccttcgcgc caggtcgcgc ccatcgaggc
     1081 gcatgcacgc gaagtaggct aacgcccgtg gcgagcgaac gggtgcacgt gctcttcgtg
     1141 gtgaccaact acccgccgca ccacgggggc gtccaggtgc acgtgcaggc cctggcccgc
     1201 gagatcgtgg cgctcggcgc atccgcgacc gtggtctgtc tcggccgtaa tcctggccgg
     1261 cgcgacgagg acggcgtgcg ggtcgtcacc ctgcaacggc acctcgggtt cgccgaggtc
     1321 ctggccctgc cggggccgcg cgagtggcgc cggcggatgc ctgacctggc gcgggaggct
     1381 acccacatct cgacgcatac ccggttcttc ccgatgtcgt ggctgggtct gcgggccggg
     1441 cgacgggccg ggcgcccggt cgtccacacc gagcacggcg ccggcttcgt gcggaccggt
     1501 gcacccgtcg tcgacctgct cgcccggctc gtcgatctca cgatggggcg ggcggtgctg
     1561 cggggagcca cccgggtcct cgccgtctcc gatgccgttg cggacttcgt gcggcacctg
     1621 gccgggcggg agagcgtcgt cgtcggcaac ggggtgcac
//
LOCUS       DATAIX010000120         1895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_6390, whole genome shotgun sequence.
ACCESSION   DATAIX010000120 DATAIX010000000
VERSION     DATAIX010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6390"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1791)
                     /locus_tag="VHM65_01880"
     CDS             complement(217..1791)
                     /locus_tag="VHM65_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2704479.1"
                     /translation="MDQTIEVGMSRLVPVGRAAVVALGAALALSSCITLPFGSDPAPT
                     STRPSSALPPPAGQEVLATFYAQRLQWQSCQGGECATLRVPIDYANPSGDTIELALLR
                     VRARSSSQRIGSIVVNPGGPGGSGVDYAKAADFIVGSSVRRRFDIVGFDPRGVGRSAP
                     IDCIDDGALDHFLGMDPTPDTGAEQQALLTEATALAEGCQERGGALLAHVSTVDAARD
                     MDVLRAALGETTLSYLGKSYGTLLGATYAGLFPRQVGRFVLDGVVPPDLTSAELSEGQ
                     ARGFEAATRAFMQDCVDDGDCPFGSTIDEGVTWLQDFLKGLDSTPVRVSADGEVTALT
                     EGWGSLGIAYALYDQGSWATLTKALQAGERGQGDDLMDLADSYADRERGGGYTGNIME
                     VIYAVNCLDRPDTADPDQIMANETAFSRKAPTWGRFLAWGSVPCGVWPLEPTGSPHKI
                     TAEGSPPIVVVGTTRDPATIYEWSVRLRGELANAVLVTYDGDGHTAYSRSSSCVDKPI
                     DEFFVTGTPPQDGLRC"
BASE COUNT          297 a          663 c          664 g          271 t
ORIGIN      
        1 gtgtgggcgg gctcgcggtc gccccgcctg cgcgccgccg agcaccgctg cggccgcgcg
       61 cgagagcgcg gcggccggga cgagaagcgg tcggtacctg gaaatggttc tcctgccttc
      121 gcctcggacg ccgtaaccgg accaggctag gtccccgcag gcgaaagcgg gaccgcttgc
      181 agaaaacgag gcgtgcgcac ctcgcgggtc ccgctcttag cagcgcaagc cgtcctgggg
      241 tggggtcccg gtcacgaaga actcgtcgat gggcttgtcg acacacgagc tggagcgcga
      301 gtaggcggtg tgaccatcac cgtcataggt caccaggacc gcgttggcca gctcgccgcg
      361 aagccgcacc gaccactcgt agatcgtggc tgggtcgcgg gtcgtcccga cgacgacgat
      421 gggcgggctg ccttccgcag tgatcttgtg cggcgacccg gtgggctcca atggccacac
      481 cccgcagggc accgatcccc acgccaggaa ccgtccccag gtcggcgcct tccggctgaa
      541 cgccgtctcg ttggccatga tctggtcggg gtcggccgtg tccggccggt cgaggcagtt
      601 gactgcgtag atgacctcca tgatgttgcc ggtataccca ccgccgcgtt cgcggtcggc
      661 atacgagtcg gccaggtcca tgaggtcgtc gccctggccg cgctcgccgg cctgcagggc
      721 cttggtcagc gtcgcccagg agccctggtc gtacagggca taggcaatcc cgagcgagcc
      781 ccagccctcg gtcagcgccg tgacctcacc gtcggcgctg acgcgcacag gagtggagtc
      841 caggcccttg aggaagtcct gcagccaggt gaccccctcg tcgatcgtgc tgccgaaggg
      901 gcagtcaccg tcgtcgacac agtcctgcat gaaggcgcgc gtggccgcct cgaagccgcg
      961 ggcctggccc tcgctcagct cggctgaggt gaggtccggc gggacgacgc cgtcgaggac
     1021 gaaccggccg acctgccggg ggaagagccc cgcgtaggtc gcgccgagga gcgtgccgta
     1081 ggacttgccg aggtagctca gcgtggtctc gccgagcgcc gcacgcagca cgtccatgtc
     1141 gcgggccgcg tcgaccgtcg agacgtgagc gagcagcgcg cccccacgct cctggcagcc
     1201 ctcggcgagc gcggttgcct cggtgagaag cgcctgctgc tcggcgcccg tgtcgggcgt
     1261 cgggtccatg ccgaggaagt gatcgagagc gccgtcgtcg atgcagtcga tcggcgcaga
     1321 ccgcccgaca ccccgcgggt cgaacccgac gatgtcgaac cgccgccgca ccgaggaccc
     1381 cacgatgaag tcggcggcct tggcatagtc gacaccggag ccacctggcc caccggggtt
     1441 gacgacgatc gacccgatcc gctgcgagga cgatcgggcc ctgactcgca acagggccag
     1501 ctcgatcgtg tcaccgctgg ggttcgcata gtcgatcggc accctgagcg ttgcgcactc
     1561 accaccctgg caggactgcc actgcagacg ctgggcgtag aaggtcgcga gcacctcctg
     1621 cccggcgggc ggcgggagcg ctgagctggg cctcgtcgag gtcggcgctg ggtcgctgcc
     1681 gaagggcagg gtgatgcagg aggacagcgc aagggctgct cccaacgcca ccaccgctgc
     1741 ccggccgacc ggcactaacc gcgacattcc cacctcaatc gtctggtcca cggacaaccc
     1801 acgttacctc gccggacttg aggtcgcccg cagggacaag gccatcgcct caagagccag
     1861 cagcgggggc acattggcgt tgatccgttc ccgag
//
LOCUS       DATAIX010000121         1679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_156013, whole genome shotgun sequence.
ACCESSION   DATAIX010000121 DATAIX010000000
VERSION     DATAIX010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156013"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..812
                     /locus_tag="VHM65_01885"
     CDS             201..812
                     /locus_tag="VHM65_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2704480.1"
                     /translation="MTSGTSSPTCSILTGGAGRRPSIRGGGWSRRCCTSPAPAASGAT
                     CPSGSRRGRRSGPTFHAAGGRGGRTFGAKRSILVEILVLPLAVRVDPARPHDVVAGRE
                     LLADRLPELPRVRAVVADRAYRGLARLAARRQLALDIKVPPPGTSGFVPLRPLYRVEH
                     AFAQLGRWRRLSRCYEGTAASARAWLEVAAMGYLFARLRVEPA"
     gene            complement(1043..>1679)
                     /locus_tag="VHM65_01890"
     CDS             complement(1043..>1679)
                     /locus_tag="VHM65_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704481.1"
                     /translation="LAGLVVDEDELLGDLTEGRALREATDGATVAGSWYRRQAVRAVP
                     RLLWRGLRTRFGWVALGVVGALAVNLAVLASLGPTGLFDSRYTPTTQEVLGDTIVHGV
                     AILLGGLLAGAVAALLSRGARLVPVVIVAAFCFLWAWHGPFYAVYDPGRLTGDGPYAI
                     ELGAWSQDLLLPAVWQLPTMSILMPTVTLLGGLAAVAWTRRRASRDGSDPA"
BASE COUNT          224 a          646 c          582 g          227 t
ORIGIN      
        1 catccaaggt cgtgggcagg tcactgggcg tcgtcgacat cggggcaggg ctgtccgacg
       61 ggagcgcgcc ctcggccccg gcccggtgac tgagggcctg tagccgaatt ccctcgcacg
      121 aatcgaccca gccgtgccat gaaagctggc acatgacggt ccgatgctgc ccgacgtggg
      181 cacgatcatc gacctctccg atgacgagtg ggacttcgtc gccgacctgt tcgatcctga
      241 cgggcggcgc gggacgccgg cccagcatcc gcggcggcgg atggtcgagg cgatgctgta
      301 cctcgcccgc accggctgcc agtggcgcta cctgcccgag cggttcccgg cgtgggaggc
      361 ggtctggtcc cacgttccat gccgctggcg ggcggggtgg ccggacgttc ggtgccaagc
      421 ggagcatcct ggtcgagatc ctggtcctgc cgctcgcggt gcgggtcgac ccggcccgac
      481 cccacgacgt ggtggccgga cgcgaactgc tcgccgaccg gctgcctgag ctgccgcgcg
      541 tgcgggccgt cgtcgccgac cgcgcctacc ggggtctcgc ccgtctcgcc gcccggcgtc
      601 agctcgcgct cgacatcaag gtcccgccgc cggggacgag cggctttgtg ccgctccgcc
      661 cgctctaccg ggtcgagcac gccttcgccc agctcggccg ctggcgccgc ctctcgcgct
      721 gctacgaggg gaccgcggca tccgcccgag cctggctcga ggtggccgcc atgggctacc
      781 tgttcgcgcg tttgcgggtc gagcctgcct gacgcgcacc ggcggtcggc ggctccgagc
      841 gcgtttcggt tgcgccgggc atcccgccac ccgtcatgtc gcggccaacc gggtacctgc
      901 agccgatctc cgcgtctccc acccgcgccg tcctgcgaat gtgagcccta cgccgtccgc
      961 catcctgtcg cgagttcagg tcggggtcgg ccgcagggcc cgaattcggc tacaggccct
     1021 cagccgacgc cctggccccg cctcacgcgg gatctgaccc gtcccgcgac gcgcgacgcc
     1081 gcgtccacgc caccgcggcg aggccaccga ggagggtcac cgtcggcatc aggatcgaca
     1141 tcgtcggcag ctgccagacg gcggggagca gcaggtcctg ggaccacgct ccgagctcga
     1201 tcgcgtacgg cccgtccccg gtcaggcggc cggggtcgta gaccgcgtag aacggtccat
     1261 gccaagccca caggaagcag aaggcggcca cgatcaccac cggcacgagg cgggcacccc
     1321 ggctcagcag cgcggcgacc gcccctgcca gcagcccgcc cagcagaatc gcgacgccgt
     1381 gaacgatggt gtcgccgagg acctcctgcg tcgtcggcgt gtaccggctg tcgaacaagc
     1441 ccgtcggacc cagcgaggcg aggaccgcaa ggttgaccgc caatgccccc acgacgccga
     1501 gcgccaccca gccgaacctg gtccgcaacc cgcgccatag caggcgtggc acggcgcgca
     1561 ctgcctggcg gcggtaccag ctccctgcga cggtggcgcc gtcggtcgcc tcccggaggg
     1621 cgcgcccctc ggtcaggtcg ccgagcagct cgtcctcgtc cacgaccagc ccggccagg
//
LOCUS       DATAIX010000122         4126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_6503, whole genome shotgun sequence.
ACCESSION   DATAIX010000122 DATAIX010000000
VERSION     DATAIX010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6503"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1498)
                     /locus_tag="VHM65_01895"
     CDS             complement(<1..1498)
                     /locus_tag="VHM65_01895"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEX2704482.1"
                     /translation="MTSTTDWVHHVRDKDVERRVGSSTFSRGRWYALQGRVRSLTHSP
                     SGAILASVRGTRPAPYEVVVTGRRDHTGVWWDGECTCPVGYECKHAVAALLAARLALR
                     PVQAPWEAVLEPLVREPEQAGPGSSTVPLALHFLVEAPRPGRFSSGVQVPRVTLRPML
                     RGKSGKWVKTGASWADLGSGYTRGPSQAQLGVIQALWQVATTGRGGYLQYSRPDRLDL
                     ASLPPAVWRVLGSAAEAGLALLSGLGQDTPVELAADPATFTIDVVADEDGSGGARVVP
                     SVSVAGVRRRGIEVGWLGDPVHGFFLQSPPAGGPGGHAGSARTGGSLTLTRLAEPLDR
                     DVSTLVLRSVEVAIPGADVGRFISLYYPRMRRRVAVESSDASVVLPQVVPPRLALDVT
                     FSGGHVADLAWSFLYGDAEAAARLPLWPEPSGVSLGSRPAARDDRAERQIVDGLDCLD
                     ELPVLATVHGGRRRMAEQARLAGHDTRVFVTGVLPRLQADERVVVTVAG"
     gene            1678..3114
                     /locus_tag="VHM65_01900"
     CDS             1678..3114
                     /locus_tag="VHM65_01900"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX2704483.1"
                     /translation="MATLRSPGVSQSLAERMKRRKPLTPGPHDTGLVRSIGLFQLTMF
                     GVGATIGTGIFFVLQTNVSKAGPAIIFSFALAAVVAGLTALCYAELASAVPVSGSSYS
                     YAYATLGEGVAMVIAACLLLEYGVSSAAVSVGWSAYLNKLLGNLFDIQIPDALSLAPA
                     EGGIINVPAMVLVGLCMLLLIRGASESAKVNAVMVLIKLGVLMLFVAIGLTGWDTNNL
                     ADFAPLGAAGIWVATGGIFFSFIGLDAVSTAGEEVKDPTRTMPRAIVLALLIVTVVYV
                     LVAVVAVAAQPWQRFEGQDAGLAQILQDVTGSTWPGTMVAGGAVISIFSVTLVTIYGQ
                     TRILFTMGTDGVVPPVFSKVNPRTLTPIQNTVIVCAAVALLAGLVPLGGLADLTSIGT
                     LAAFSVVSLAVILLRRRQPDMHRAFKVPGYPVTPILSIAACIVVMSGLSAITWIFFAG
                     WVGAFLVFYFLYGIKHSNLEPYPKGDEQ"
     gene            3483..3983
                     /locus_tag="VHM65_01905"
     CDS             3483..3983
                     /locus_tag="VHM65_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2704484.1"
                     /translation="MHSSQVAVAVAPRGYRSGAGQRVERVTVAVDGTAATQLVVQRAA
                     SVAAQVGARLRLVTFAVRAGTMFPPEVGLHAEDKVVQVWREDADRIVHTALADLGQTL
                     VGEPEYGVADARTWAEALDEPGWVPGDVLVIGSSSSQSLVSRVFLGSTAARILRHSPV
                     PVVVVP"
BASE COUNT          556 a         1484 c         1469 g          617 t
ORIGIN      
        1 cgcccgccac cgtcacgacg acccgctcgt cggcctggag cctgggcagc accccggtga
       61 cgaagacccg cgtgtcgtga ccagcgagcc gggcctgctc ggccatccgg cgccgtccgc
      121 cgtgaacggt ggcgagcacc ggcagctcgt ccaggcagtc cagcccgtcg acgatctggc
      181 gttcggcccg gtcgtcacga gcggccgggc gcgaaccgag cgagacaccg gacggctccg
      241 gccacaacgg caggcgcgcg gccgcctcgg cgtcgccata caggaaggac caggccaggt
      301 cggcgacgtg gccgccggag aaggtgacgt cgagggcgag ccgagggggc acgacctgcg
      361 ggaggaccac actcgcgtcg gaggactcga ccgcgacccg gcggcgcatc ctcgggtagt
      421 agagggagat gaagcggccc acgtccgcac cggggatggc gacctcgacc gagcggagca
      481 cgagcgtgct gacatcgcgg tcgaggggct cggcgagccg ggtcagggtg aggctcccgc
      541 cggtgcgggc ggagccggcg tgaccgccgg ggccaccggc cggaggggac tggaggaaga
      601 acccgtggac cgggtccccg agccagccca cctcgatgcc ccggcggcgg acgccggcca
      661 cggagacgct cgggacgacc cgagccccgc ccgagccgtc ctcgtcggcg acgacgtcga
      721 tcgtgaaggt ggcggggtcg gcggcgagct cgacgggcgt gtcctggccc aggcccgaca
      781 acagcgccag cccggcctcg gcggcggacc cgagcacccg ccataccgcc ggggggaggc
      841 tcgccaggtc gaggcggtcc ggacgggagt actgcaggta gccgccgcga ccagttgtcg
      901 cgacctgcca gagggcctgg atgacgccga gctgcgcctg gctcggcccg cgggtgtagc
      961 cgctcccgag gtcggcccac gaggcgccgg tcttgaccca cttgccggac ttgccgcgca
     1021 gcatcgggcg cagcgtgacc cgggggacct ggacgccgct ggagaagcgt ccgggcctgg
     1081 gcgcctccac gaggaagtgc agggccaggg ggacggtgct cgagcccggg ccggcctgct
     1141 ccggctcccg cacgagcggc tcgagcaccg cctcccacgg cgcctgcacg gggcgcaggg
     1201 ccaggcgcgc ggcgagcagc gcagccaccg catgcttgca ctcgtagccg accgggcagg
     1261 tgcactcgcc gtcccaccac acgccggtgt ggtcgcgacg gccggtgacg acgacctcgt
     1321 acggcgcggg cctggtcccc ctgacggagg ccaggatcgc ccccgacggg gagtgggtca
     1381 gggacctgac ccggccctgc agggcatacc agcggccccg gctgaacgtg ctggacccga
     1441 cgcggcgctc gacgtccttg tcgcgcacgt ggtgcaccca gtcggtggtc gaggtcacgg
     1501 acaggagcct acggccacat ggtgacacga cccgccacgg ctggccgcgc gccgccgccg
     1561 gcctggcccg ccagggaaga aaatcgcaga attgtcgacc gcgccgtatt gacgccggtc
     1621 tgtcgtggcg gccagagtca gaggcttatc gcggtccagg accctgtgga ggtcggtatg
     1681 gcgacgttgc ggtccccggg agtatcccag tcgctggctg agcggatgaa gcgccgcaag
     1741 ccactcaccc cgggaccgca cgacaccggg ctggtgcgct cgatcggcct gttccagctg
     1801 acgatgttcg gcgtcggggc cacgatcggc acgggtatct tcttcgtcct gcagacgaac
     1861 gtctccaagg ccggccccgc catcatcttc tccttcgccc tggcggcggt cgtcgcggga
     1921 ctcaccgcgc tgtgctacgc cgagctcgcc agcgcggtgc cggtctcggg ctcgtcctac
     1981 tcctatgcct acgcgacgct cggggagggc gtcgcgatgg tcatcgccgc ctgcctgctg
     2041 ctggagtacg gcgtgtcgtc ggcggcggtc tcggtgggct ggtcggcgta cctcaacaag
     2101 ctgctgggca acctcttcga catccagatc cccgacgcgc tgtccctggc cccggccgag
     2161 ggcgggatca tcaacgtgcc ggcgatggtg ctcgtcggcc tgtgcatgct cctgctcatc
     2221 cgcggggcga gcgagtcggc caaggtcaac gcggtcatgg tcctcatcaa gctcggcgtg
     2281 ctcatgctct tcgtcgccat cggcctgacc gggtgggaca ccaacaacct tgccgacttc
     2341 gcccccctcg gcgccgcggg catctgggtg gcgaccggcg ggatcttctt ctccttcatc
     2401 ggcctcgacg ccgtctcgac ggcgggcgag gaggtcaagg accccacccg caccatgcct
     2461 cgtgccatcg tcctcgcgct gctcatcgtc accgtggtct acgttctcgt cgcggtcgtc
     2521 gccgtcgccg cccagccgtg gcagcggttc gaggggcaag acgccggcct ggcccagatc
     2581 ctccaggacg tcacggggtc gacgtggccg ggcaccatgg tcgccggggg cgcggtcatc
     2641 tcgatcttct cggtcaccct cgtgacgatc tacggccaga cccggatcct gttcaccatg
     2701 ggcaccgacg gtgtcgtgcc gccggtgttc agcaaggtca acccccgcac gctgaccccg
     2761 attcagaaca ccgtcatcgt ctgcgcggcg gtggcgctgc tcgccggcct ggtcccgctc
     2821 ggcgggctcg ccgacctcac gagcatcggc accctggcgg cgttcagtgt cgtctcgctg
     2881 gccgtgatcc tcctgcggcg gcgccagccg gatatgcacc gggccttcaa ggtccccggc
     2941 tacccggtca caccgatcct gtccatcgcc gcgtgcatcg tcgtcatgtc cgggctgagc
     3001 gcgatcacgt ggatcttctt cgccggctgg gtcggcgcct tcctcgtctt ctacttcctc
     3061 tatgggatca agcactccaa cctcgagccc taccccaagg gtgacgagca gtgacgatcg
     3121 tcgtcgggta cggaccggag gaccgcggcc acggtgccct ccagctggcc cggatgctcg
     3181 ccacgtcggc cggcgtcggg ctcgtcgtct gctgcgtcat ccccgaccgg tggcagtcgg
     3241 tgggtccggg ccggcaggtc gaccgcgact acgaggagta cctccgcgga ctcgctcgac
     3301 cacgccgcgg cgctcatcgg agacctcacg gtcccggtca cctacgacgt gctcacctcg
     3361 cgctccgcgc cgtccggcct gctgctagca gccgagcgct ttgacgcgac ggtactcgtc
     3421 gtcggctcct cgtcgcacgg cgcctggggc atgtcgcgct cggcagcgtc acggaccggt
     3481 tgttgcacag ctcgcaggtg gccgtcgcgg tggccccccg tggctaccgc agcggggctg
     3541 ggcagcgggt ggagcgcgtc accgtggcgg tcgacggcac cgcagcaacc cagctggtgg
     3601 tccagcgggc ggccagtgtc gcggcgcagg tcggcgcccg gctgcgcctg gtgaccttcg
     3661 cggtgcgggc ggggacgatg ttcccgccgg aggtgggcct gcatgccgag gacaaggtcg
     3721 tgcaggtttg gcgcgaggac gccgatcgca tcgtgcatac cgcgctggcg gatctgggcc
     3781 agactctcgt cggggaaccc gagtatgggg tcgccgatgc ccgcacctgg gccgaggcgc
     3841 tcgacgagcc ggggtgggtt ccgggggacg tgctcgtcat cgggtccagc tcgtcccagt
     3901 cgctggtctc ccgggtgttc ctcgggtcga ccgccgcccg gatcctgcgg cactccccgg
     3961 tcccggtcgt cgtcgtcccc tgacgaccga tgtgtaccgg ctcggggcga gcgtgccgag
     4021 gtagccgccc tcgcctgatc gtaccgatct ctactgcact atagtgaaac gctataggcg
     4081 gctaccattt gctacacatg gagcggtatg gacccagtgc tcaacc
//
LOCUS       DATAIX010000123         1958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_6525, whole genome shotgun sequence.
ACCESSION   DATAIX010000123 DATAIX010000000
VERSION     DATAIX010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6525"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..991)
                     /gene="miaA"
                     /locus_tag="VHM65_01910"
     CDS             complement(77..991)
                     /gene="miaA"
                     /locus_tag="VHM65_01910"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX2704485.1"
                     /translation="MTGVVVAVVGPTAVGKSALSLDLAQALGGEVVNADAMQLYRGMD
                     IGTAKLTAAERRGVRHHLLDVLDVREEASVATYQRVSRAAVADIHSRGRVPILVGGSG
                     LYLRAALDRLEIPPTDPRVRHRIEAEYAAHGLAPVLDRLTVLDPAAAAHINPANARRV
                     IRALEVVELTGRPFSATMPQREYVVPTVVIGLTAARPVLDERIERRVGAMWEAGLLGE
                     VRALAGQGLREGRTASRALGYAQALAVLDGGLAPAQAQADTVALTRRFARRQQSWFRP
                     DPRIAWLAHDRPDLKASAVELARRHTGQ"
     gene            complement(988..1671)
                     /gene="def"
                     /locus_tag="VHM65_01915"
     CDS             complement(988..1671)
                     /gene="def"
                     /locus_tag="VHM65_01915"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX2704486.1"
                     /translation="MAIRPIVITGDPVLHRPAARVERFDAALRSLIGDLVETMDAAHG
                     VGLAAPQIGVGLRIFTYAIPNDDGVLPRGVVVNPILAVGKIPKERPDPDEESEGCLSV
                     PGQSFPLKRAGWVKVSGFGAAGEPLEFEATGWFARCIQHEFDHLNGKLYVDRLTDRWA
                     RKARKAARSRGWGKPGLSWRPGVDPDPFGHDHEVGDHDFAAHGPDAHVEGGPAPATEY
                     ATDEVSDPA"
BASE COUNT          293 a          669 c          725 g          271 t
ORIGIN      
        1 ccggccaggt cggggagcag gacgaagtcg ttctcggtcc cgtggccctt ggtgaaacgc
       61 aggctcgcca tggtcgtcat tgtcccgtat gccgtcgcgc cagctcgact gcgctggcct
      121 tgaggtccgg gcggtcgtgg gccagccacg cgatcctggg gtcgggccgg aaccacgact
      181 gctggcgccg ggcgaaccgc cgggtcagcg cgaccgtgtc ggcctgggcc tgtgctgggg
      241 cgagcccgcc gtcgaggacg gcgagggcct gggcatagcc gagggcccgc gaggcggtcc
      301 tgccctcccg caggccctgc ccggccaggg cgcggacctc gccgagcagc ccggcctccc
      361 acatcgcgcc cacccggcgc tcgatgcgct cgtcgaggac cggccgagca gcggtgagac
      421 cgataacgac ggtcgggacg acatactcgc gctggggcat cgtggcactg aacggccgcc
      481 cggtcagctc gacgacctcg agcgcccgga tgacgcgccg ggcgtttgcc gggttgatgt
      541 gtgctgcggc ggcggggtcg agcacggtca gccggtccag caccggcgcc agaccgtggg
      601 cggcatactc ggcctcgatc cggtgccgga cccgcgggtc ggtcggcggg atctcgagcc
      661 ggtcgagggc ggcccgcagg tagaggccgg agccgccgac gaggatcggc acgcggcctc
      721 tggagtggat gtcagccact gcggcccgcg aaactcgttg gtatgtcgcg acactcgcct
      781 cctcgcgcac gtcgaggacg tccaggaggt gatggcggac gccccggcgc tcggctgcgg
      841 tgagcttggc cgtgccgata tccatgcctc ggtagagctg catcgcatcg gcgttgacga
      901 cctccccgcc gagggcctgg gcgaggtcga gcgacagggc gctcttgccg acggccgtag
      961 ggccgacgac cgcgacgacg acaccggtca cgctgggtcg gacacctcgt cggtcgcata
     1021 ctcggtggcc ggtgccggac cgccttcgac gtgggcgtcc gggccgtgcg cggcgaagtc
     1081 gtggtcaccg acctcgtggt cgtgcccgaa cgggtcgggg tcgacaccag gacgccagga
     1141 cagcccgggc ttgccccacc ccctggacct ggccgccttg cgggccttgc gggcccagcg
     1201 gtcggtgagg cggtcgacat acagcttgcc gttgaggtgg tcgaactcgt gctggatgca
     1261 gcgggcgaac cacccggtgg cctcgaactc gagcggctcc cccgcggcgc cgaaaccgct
     1321 caccttgacc cagccggccc gcttgagcgg gaagctctgg ccagggacgg acaggcagcc
     1381 ctcggactcc tcgtcggggt cgggccgctc cttggggatc ttgccaacgg cgaggatcgg
     1441 gttgactacg accccgcgcg gaagcacgcc gtcgtcgttg ggtatggcgt aggtgaagat
     1501 ccgcagcccg acaccgatct gtggggccgc caggccgacg ccgtgagcgg catccatcgt
     1561 ctcgaccagg tcgccgatga ggctgcgcag cgcagcgtcg aaccgctcga ctcgggcggc
     1621 cgggcggtga agcaccggat ctccggtgat gacgatgggt cggatggcca tgagcgccat
     1681 gttctcagac ccgggacggc cggccgtcac gccgccacga cacacccccg gcccggcgaa
     1741 ttggtgcata gcccgcgaat gcgacatggt agaagggcga gcgcgatgcg tgctcgtgcc
     1801 agcaccacca agcaaggagt tttgccgtga acatgttcga caacctcaag gacaagacca
     1861 aggagctctt cggccagcac tccgacaagg tcgaggagct gtccgaccag ggcatcgaga
     1921 aggcggccga cgcggccgac gcggccacgg gaggcaag
//
LOCUS       DATAIX010000124         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_96366, whole genome shotgun sequence.
ACCESSION   DATAIX010000124 DATAIX010000000
VERSION     DATAIX010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96366"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VHM65_01920"
     CDS             <1..408
                     /locus_tag="VHM65_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2704487.1"
                     /translation="SLLWAALRGVAARHVAELDELAAAYLGDRSDLQTITRRDLQQRL
                     AAGDIVVLDVRPGAEYAAGHIAGAVSVPLAELEDRLADLPEGTAVAAYCRGPYCVYAD
                     DAVRMLGARGFRAARLEDGYPEWARAGLTVSCG"
     gene            complement(462..1235)
                     /locus_tag="VHM65_01925"
     CDS             complement(462..1235)
                     /locus_tag="VHM65_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX2704488.1"
                     /translation="MTARTHTHTPLTVLTPPRHRTLAQRAQLLAAASVTYNVAEAVIA
                     LSAGTVAGSTALIGFGLDSTVEVASGLVILWQFRHPMPETRERRAQRLIAVSFFALAA
                     YVVVESVRALLGAERPDSSPVGIGLALASLAVMPLLSWAQRRTGRELGSGAVVADGTQ
                     TLLCTYLSAVLLLGLLANALLGWWWLDASAALVIAAVAVREGLQSWRGEGCCAPEAVV
                     GAGPDSGDPDSGDPGGRDDCCAGRGAGHEPGGGCCRAGR"
     gene            complement(1232..>1537)
                     /locus_tag="VHM65_01930"
     CDS             complement(1232..>1537)
                     /locus_tag="VHM65_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704489.1"
                     /translation="VMLGREVLAVLGQHAPADVEPTEMVEALSAVHDVLDVHDLHVWS
                     LTSGMTVASAHLVARDQADSAAVLDTAQEVLRDRFSIAHATLQVEAAGRAQCQGARW"
BASE COUNT          216 a          582 c          561 g          178 t
ORIGIN      
        1 tcgctgctct gggccgctct gcgcggcgtc gcggcacggc acgtggccga gctcgacgag
       61 ctcgccgccg cctacctcgg ggaccgcagc gacctgcaga cgatcaccag gagggacctg
      121 cagcagcgcc tcgcggccgg ggacatcgtc gtcctggacg tccggccggg cgccgagtac
      181 gccgccggtc acatcgccgg agccgtctcc gtccccctcg ccgagctcga ggaccgcctg
      241 gcggacctgc cggaaggtac cgccgtcgcc gcctactgcc gtggcccgta ctgcgtctac
      301 gccgacgatg ccgtccgcat gctcggcgcc cgcgggttcc gcgccgcacg gctcgaggac
      361 ggctaccccg agtgggcccg ggccgggctc acggtgtcat gcggctgagc gcaccgcgac
      421 ggcccggggg gagcctgccc aaccgggcct gcccgccttc ctcaccggcc agcgcggcaa
      481 caaccaccgc ccggctcatg gcccgcgccc ctccccgcgc agcagtcgtc gcgaccgccc
      541 gggtcgccag agtccgggtc gccggagtcc ggtcccgcac ccaccacagc ctcgggcgcg
      601 cagcagcctt cgccgcgcca gctctgcagg ccctcgcgca ccgcgacggc ggcgatgacg
      661 agggcggctg acgcgtccag ccaccaccag cccaggagtg cgttggccag caggcccagc
      721 agcagcaccg ctgagaggta ggtgcacagg agcgtctggg tgccgtcggc gaccaccgcg
      781 cccgagccga gctcacggcc ggttcggcgc tgtgcccacg agagcagcgg catgacggcc
      841 agggacgcca gggccaggcc gatccccacg ggtgaggagt cgggccgctc cgcgccgagc
      901 agggcgcgga cggactcgac gacgacatac gcggcgaggg cgaagaagga cacagcgatc
      961 agacgctgcg cgcgccgctc cctcgtctcg ggcatggggt ggcggaactg ccacaggatg
     1021 accagcccgc tggccacctc gacggtggag tcgaggccga agccgatcag cgcggtcgag
     1081 ccggccacgg tgcccgcgga gagggcgatg accgcctcgg cgacgttgta ggtcaccgag
     1141 gccgcggcga gcagttgggc gcgctgggcc agtgtccggt gccgcggcgg ggtcaggacc
     1201 gtgagcgggg tgtgggtgtg ggtgcgggcg gtcaccagcg cgccccctgg cactgggcgc
     1261 gccctgcggc ctcgacctga agcgtcgcgt gggcgatcga gaaccggtcg cgcagcacct
     1321 cctgggccgt gtcgagcaca gcggcggagt ccgcctggtc ccgcgcgacg aggtgcgcgc
     1381 ttgccaccgt catcccagag gtcaggctcc acacatgcag atcgtggacg tccagcacat
     1441 cgtgcaccgc actgagcgcc tccaccatct ccgtgggctc gacatcggcg ggcgcgtgct
     1501 ggcccagcac agccaggacc tctcggccga gcatgac
//
LOCUS       DATAIX010000125         1940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_36866, whole genome shotgun sequence.
ACCESSION   DATAIX010000125 DATAIX010000000
VERSION     DATAIX010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36866"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VHM65_01935"
     CDS             complement(<1..483)
                     /locus_tag="VHM65_01935"
                     /inference="COORDINATES: protein motif:HMM:NF019559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HEX2704490.1"
                     /translation="MNIYAWVLLGALAVAAPADWVCVHRGFREGERLAAPAFMVLLLA
                     LAWLLGADLVGYGRWLLIGLVASLVGDLFLLGDTEERFRAGLGSFLAVHLAYLAMILT
                     LPSHGSPLWVSVVLVALTAGVASYRLILPLVREVPGEGVPLLGYALVLALVAMAAWWH
                     G"
     gene            476..955
                     /locus_tag="VHM65_01940"
     CDS             476..955
                     /locus_tag="VHM65_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704491.1"
                     /translation="MFTHLTLSDPNPDAADPEPVGWAGMNTMEHTVTEADTAEHLGSG
                     DLPVLGTPRLINWAEVATVAAAAEALDEDHTTVGTLVKFEHVRASPVGATITITCSKP
                     VNDGRRLIFHVRVADSAGDELAHGEIHRAVVDRQRFMARLDEAGEPPVTSPTPDSRA"
     gene            1013..1474
                     /locus_tag="VHM65_01945"
     CDS             1013..1474
                     /locus_tag="VHM65_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704492.1"
                     /translation="MNFVERLEAQSSVFVEEAAAALERQGMKHYVDSGAESTRDRMQE
                     LMDLLLECLRGKTLLPMVKHAERVANERYRAGFGLEEVQTSFNVMEESLWKHLVHEAP
                     VAELAEDLGLIGTVLGAGKDQLARTYVSLASHHHAPSLDLHALFGGAAHGA"
     gene            complement(1589..>1940)
                     /locus_tag="VHM65_01950"
     CDS             complement(1589..>1940)
                     /locus_tag="VHM65_01950"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX2704493.1"
                     /translation="ARQVGSGWSGLNPVLAAGDVDSDGHPDLLARTSTGALLLYPGSS
                     SGGFLHSRQVGHGWSGFTSIVSVGDFDGTGTSDVLARRADGTLWLYPGLGTGRWGTAR
                     QVGSGWNGIEPLVP"
BASE COUNT          337 a          714 c          660 g          229 t
ORIGIN      
        1 gccgtgccac cacgcggcca tggcgaccag ggcgaggacg agcgcatacc cgagcagggg
       61 gaccccctcg cccggcacct cgcggaccag cggcaggatg agccggtatg acgccacgcc
      121 cgccgtgagt gccacgagca cgaccgacac ccagagcggg gacccatggc tcggcagggt
      181 caggatcatc gccaggtagg ccagatggac ggccaggaac gaacccagcc cagcccggaa
      241 gcgctcctcg gtgtcgccga gcaggaagag gtctccgacc aggctcgcga ccagcccgat
      301 gagcagccag cggccgtagc ccacgaggtc cgcaccgagg agccaggcca gggcgagcag
      361 gagcaccatg aacgcgggcg cggcgagccg ctcgccctca cggaacccgc ggtggacaca
      421 cacccagtcg gccggggcag ccacggcgag tgctccgagg agcacccagg cgtagatgtt
      481 cacgcacctc accctgtcag acccgaaccc ggatgccgcg gacccggagc ccgtaggatg
      541 ggcgggcatg aacaccatgg agcacacggt cacggaggcc gacaccgctg agcacctcgg
      601 cagcggtgac ctccccgtcc tggggacgcc ccggttgatc aactgggccg aggtcgccac
      661 ggtggccgcg gcggccgagg cgctcgacga ggaccacacg accgttggca ctctcgtcaa
      721 gttcgagcac gtcagggcca gcccggtcgg tgcgacgatc acgatcacct gctccaagcc
      781 ggtcaacgac ggccgccggc tgatcttcca tgtccgcgtg gccgactccg ctggtgacga
      841 gctcgcccat ggcgagatcc accgggccgt cgtcgaccgg cagcgtttca tggcccgact
      901 cgacgaggcc ggcgagccgc ccgtgacctc ccccacgcct gactcccggg cctgagcgca
      961 cgtcgcggga gtagggtgag ggaccgactt aggcaactga agggacaccg tcatgaactt
     1021 cgtcgagcgg ctggaagccc agtcgtcagt cttcgtcgaa gaggcggcgg ccgcgctcga
     1081 acgccagggc atgaagcatt acgtcgactc cggtgcggag tcgacgcgcg accgcatgca
     1141 ggagctgatg gacctgctct tggagtgcct gcgcggcaag accctgctgc ccatggtcaa
     1201 gcacgccgag cgcgtggcga acgagcgtta ccgggcgggc ttcgggctcg aagaggtcca
     1261 gacgtcgttc aacgtcatgg aggagtcgct ctggaagcac ctcgtccacg aggcaccggt
     1321 cgccgagctc gccgaggacc tcggcctcat cggcaccgtc ctcggcgccg gcaaggacca
     1381 gctggcccgc acctatgtgt cactggccag ccatcaccac gcgcccagcc tggacctgca
     1441 tgcccttttc ggcggggcgg cgcacggagc ctgagcgcga cgctcggccg acctccccga
     1501 gcgcggccgc cgcaccgcac gccatgacgg ttcggccccg gcgacgacca gatctcatcc
     1561 gccaccgctc cccccggctc ctgcccgatc agggcacaag gggctcaata ccgttccagc
     1621 cggaccccac ctggcgggcg gtaccccacc gtccggtccc caggccggga tagagccaca
     1681 gtgtgccgtc ggccctcctg gccaggacgt ccgaggtccc ggtgccgtcg aagtcgccga
     1741 cggacacgat cgaggtgaac ccggaccagc cgtgcccgac ctggcgcgaa tgcaggaagc
     1801 cgcccgagct gctccccggg tagagcagca gggccccggt cgacgtacgg gctaacaggt
     1861 ccgggtgccc gtcgctgtcc acgtccccgg ccgcgaggac ggggttgagg cccgaccagc
     1921 ccgagccgac ctggcgcgcg
//
LOCUS       DATAIX010000126         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_241528, whole genome shotgun sequence.
ACCESSION   DATAIX010000126 DATAIX010000000
VERSION     DATAIX010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241528"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            390..1412
                     /locus_tag="VHM65_01955"
     CDS             390..1412
                     /locus_tag="VHM65_01955"
                     /inference="COORDINATES: protein motif:HMM:NF024801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX2704494.1"
                     /translation="MSESPLTSYVEPTFVRDTRYLDARITADGRDGWPVASGRYRLVA
                     ARACPWAHRTVIVRRLLGLEDAISLGLCGPTHEASSWTFDLDPGGLDPVLRIPRIKDA
                     YEKAVPGYPRGITVPCIVDIPSGAVVTNDFKAITLDFETQWTAFHRPGAPDLYPEPLR
                     AQIDEVAEVVYRDVNNGVYRAGFAGTQSAYDAAYATLFARLDWLSDRLAQQRYLVGDT
                     VTEADIRLFTTLVRFDPVYHGHFKCNRSKLSELPVLWAYARDLFQTPGFGDTVDFEQI
                     KAHYYVVHRDINPTGVIPAGPDLAGWHTPHGRERLGGRPFGDGTAPGPVRAGEEIRGW
                     PPQPRA"
BASE COUNT          236 a          633 c          484 g          222 t
ORIGIN      
        1 cggcctgtcg atcgggctca ccgagtccca gacgctgcgc tcgatccagc tcccgcaggc
       61 cctcaccgcc atgcttccgg ccatcgtcgg ccagctcgtc gtcgtgctca aggactcggc
      121 cctcggccag atcatcacct agcccgagct gctcaacacc ttccagcaga tcggcagcta
      181 caagtccaac ccggtgccgg cgctcatggt catcgccacc atcttcgtgg cgatcaacta
      241 ctcgctgacc acgctcgccg gccgggccta gcgacggctg cgctcctcgg gccgcccgac
      301 cctcggaggc agcccagcgg ccggcgcgat cggggtcggg gacgacctcc ccgagccgta
      361 ggcacgggcc cgcaccgtag cctgacggca tgagcgagag cccgctgacg tcatacgtcg
      421 aaccgacctt tgtccgcgac acgcgctacc tcgacgcccg gatcacggcc gacgggcgcg
      481 acgggtggcc ggtggcgtcc gggcgctacc gcctcgtcgc cgcgcgggcg tgcccgtggg
      541 cgcaccggac ggtcatcgtg cgccgcctgc tcgggctcga ggacgcgatc tcgctgggcc
      601 tgtgcggccc gacgcacgag gcgagctcct ggacgttcga cctcgacccg ggcggtctcg
      661 acccggtcct tcggatcccg cggatcaagg acgcctacga gaaagccgtg cccggctacc
      721 cccggggcat caccgtgccc tgcatcgtcg acatcccgag cggggcggtc gtcaccaacg
      781 acttcaaggc catcacgctc gacttcgaga cccagtggac ggcgttccac cgccccggtg
      841 cacccgacct ctaccccgag ccactgcgtg cgcagatcga cgaggtcgcc gaggtcgtct
      901 accgcgacgt caacaacggc gtctaccgcg ccgggttcgc tggcacacag agtgcgtatg
      961 acgccgccta cgccacgctc ttcgcccgcc tcgactggct ctccgatcgc ctggcccagc
     1021 agcggtacct ggtcggcgac acggtcaccg aggccgatat ccggctcttc accaccctcg
     1081 tccgcttcga cccggtctac cacggccact tcaagtgcaa ccgctccaag ctcagcgagc
     1141 tgccggtgct gtgggcctac gcccgcgacc tcttccagac cccgggattc ggcgacacgg
     1201 tcgacttcga gcagatcaag gcgcactact acgtcgtcca tcgcgacatc aacccgaccg
     1261 gcgtcatccc ggccggtcct gatctggctg ggtggcacac gccgcatggg cgcgagcggc
     1321 tcggcggtcg cccgttcggc gacggcaccg cgccggggcc ggtccgcgcc ggcgaggaga
     1381 tccgcggctg gcccccgcag ccccgcgcgt aaccagcctc gatcccgcac gcccggcata
     1441 ccgaggccga tctggccgcc ccgcaccggc cggatcaacc tcgatcccgc acgcccggca
     1501 taccgaggcc gatctggccg cccccgaccg gccggatcaa cctcgatccc gcacgccgac
     1561 ataccgaggc cgatc
//
LOCUS       DATAIX010000127         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_271314, whole genome shotgun sequence.
ACCESSION   DATAIX010000127 DATAIX010000000
VERSION     DATAIX010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271314"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1602
                     /locus_tag="VHM65_01960"
     CDS             <1..1602
                     /locus_tag="VHM65_01960"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HEX2704495.1"
                     /translation="QAGAGFQDNTDGPDVARDFLTPGAAAGWKPTSSVTVIEAPVAVE
                     AVDGSGVRVRAKAVARIDSAGHYTEVRPGTMVTAVFAVVRLAGEWRLDLPEGRFGLWL
                     TSQDLQRLFQPVRIYYATLSGRELVPETRWFPSGARLATTLARAQAEPVPAYLRGALT
                     TGFPAVTSLQVDAVPVLGGRANVTLTAAALQANPERRRMMYAQMVTALSQVLNVQEVG
                     LLAGDTKLELSGLGDSARSVTELGYRVVGPASPRTGLLRQGSALSSVNLQALAGLEQD
                     TRPKAVTAPAATWPAIALGWTALALSVDGAELAGVGGDLKELRRWRGTTEIRVGPFAS
                     ALTRPSYDAHGSLWTSGVSGGTTRVYVVDTAARGSAAPVAVKADWLAARRVAALRVSP
                     DGTRAAVVSREPGASTDSLDLAGIIRTLAGQPVGLSAPGPEGQPLSQVRDVTWLDIDT
                     LGVLGRVSASEPWRPYGVDLGQGIGLRSPGSALLSPVPDGQAITSAGGPRGVLVRTDS
                     GRVKVRAGSSWVQLAAGSEVIVPGA"
     gene            1696..2490
                     /locus_tag="VHM65_01965"
     CDS             1696..2490
                     /locus_tag="VHM65_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX2704496.1"
                     /translation="MPQPSAPRATRPTCSQSLRAALASLLDLALPAECAGCSRPGPAL
                     CRPCQLAFDAEHLRGGPCVVVPSPEPPGLPPVLSTFRYGGAVTTALRAYKDAGRRDLV
                     HTLAPALAPALAVALQVAVRDPSSRGSPVWVVPAPSSRSAGRRRGDRPTEQLARRALE
                     VSATSYLVPEGMVVRAVEAVRRVADQAALGHDARAVNVAFAYDVRPGLGSSMRGRVCV
                     LVDDVVTTGATLAEAARALRRAGAVVPVAATVAATALRREVPRRWT"
BASE COUNT          296 a          981 c          953 g          365 t
ORIGIN      
        1 caggccggcg cgggatttca ggacaacacc gacggccccg acgtcgcccg cgacttcctc
       61 actcccggcg ccgctgccgg gtggaagccc acctcgtcgg tcaccgtcat cgaggcgccg
      121 gtcgcggtgg aggccgtcga cgggagcggc gtccgcgtgc gcgccaaggc ggtggcgcgg
      181 atcgactcgg ccggccacta caccgaggtc aggccgggga cgatggtcac cgccgtcttc
      241 gccgtcgttc gcctcgcggg ggagtggcgt ctcgacctgc ccgagggcag gttcgggctc
      301 tggctcacct cgcaggacct ccagcgactc ttccagcccg tccggatcta ctacgcgacc
      361 ctctcgggcc gcgagctcgt cccggagacc cgatggttcc cctccggcgc ccggctcgcc
      421 accaccctgg cccgcgccca agcggagccg gtcccggcat acctgcgtgg tgctctgacc
      481 acggggttcc ccgcagtgac ctcgctgcag gtcgacgccg tgcccgtcct cggcggccgg
      541 gcgaacgtca cgctcaccgc cgctgcgctg caggccaatc ctgaacggcg ccgcatgatg
      601 tacgcccaga tggtcacggc actgagccag gtgctcaacg tccaggaggt cggcctgctc
      661 gccggggaca ccaagctcga gctgagcggc ctcggggact ccgcccggtc ggtcaccgag
      721 ctcggctacc gcgtcgtcgg cccggcgagc ccgaggacgg gtctgctgcg ccaggggtcg
      781 gccctgtcgt cggtcaacct gcaggcgctg gccggcttgg agcaggacac ccgccccaag
      841 gccgtcacgg cgcccgccgc gacctggccc gcgatcgccc tgggctggac cgcgctcgcg
      901 ctgtcggtcg acggcgccga gctagcgggc gtgggcggag acctcaagga gctgcgccgc
      961 tggcgtggaa ccaccgagat ccgcgtcggg ccgttcgcct cggcgctgac ccgcccgtcc
     1021 tacgacgccc atgggtcctt gtggacgagc ggtgtgagcg gggggacgac cagggtgtat
     1081 gtcgtcgaca ccgcggcccg gggtagcgcg gcgcccgtgg cagtcaaggc cgactggctc
     1141 gccgcgcgcc gggtggctgc gctgcgggtc tccccggatg gcacgcgggc cgccgtcgtg
     1201 tcccgtgagc ccggcgcctc gaccgactcc ctcgacctgg ccggcattat ccggaccctc
     1261 gccgggcagc cggtgggcct gtctgccccc gggccggaag gccagccact gagccaggtc
     1321 cgggatgtca cctggctcga catcgacacg ctcggagtgc tcggccgggt gagcgcgagc
     1381 gagccgtggc gaccctacgg cgtcgacctc ggccagggca tcggcctgcg atcgccgggt
     1441 tccgcgctgc tctcgccggt cccggacggc caggcgatca cgagtgctgg cggtccccgt
     1501 ggggtgctcg tccggaccga cagcggccgg gtcaaggtcc gggccggcag ctcgtgggtc
     1561 cagctcgccg ccgggtccga ggtcatcgtc cccggcgcct gaacggggcc cacaaccccc
     1621 tcgcacgcag tcccgccgtc cacagccgtg ccgaccgggc tggcgtccgg gaccacgccg
     1681 cgtcaccgta gccaggtgcc ccagccgtcc gcgccgcgcg ccacgaggcc cacctgctcc
     1741 cagagcctgc gggcggcgct cgcgagcctg ctcgacctcg cccttcccgc cgagtgtgcg
     1801 gggtgctcga ggcccgggcc ggcgctgtgc cggccgtgtc agctggcatt cgacgccgag
     1861 cacctgcgtg gcggtccgtg tgtcgtcgtg ccctcgcccg agcccccggg gcttccgccg
     1921 gtgctctcga cgttccggta tggcggggcc gtcaccaccg cgctgcgggc ttacaaggac
     1981 gccggacggc gcgacctcgt gcacacgctg gcgccggcgc ttgcaccggc tctcgcggtc
     2041 gccctgcagg tcgcggtgcg cgatccgtcc tcccgcggat cgcccgtgtg ggtcgttcct
     2101 gcgccgtcat cccggtcggc ggggcgccgc cgcggggacc ggccgaccga gcagctggcc
     2161 cgtcgcgcgc tcgaggtgag cgccacgtca tacctcgtcc cggaggggat ggtggttcgc
     2221 gcggtcgagg cggtgcgccg cgttgcggac caggcagctc tcggtcacga cgcacgggcc
     2281 gtcaacgtgg cattcgcgta tgacgtccgc cccgggctgg ggagctcgat gcgcgggcgc
     2341 gtctgcgtgc tcgtcgacga cgtcgtgacc accggggcga cgctcgccga agccgcccgg
     2401 gccctgcggc gggctggcgc agtcgtcccg gtcgcggcaa cggtcgcggc caccgcgttg
     2461 cgacgggagg tgccacggcg ttggacgtga cgccacggcg acctgagcat gacggcgaca
     2521 ccgcggcgag atagcgtttg cgtatggact ccgttcacgc gacgcacgag tcggggtgat
     2581 gccgccggtg ttgag
//
LOCUS       DATAIX010000128         2960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_66939, whole genome shotgun sequence.
ACCESSION   DATAIX010000128 DATAIX010000000
VERSION     DATAIX010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66939"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VHM65_01970"
     CDS             <1..684
                     /locus_tag="VHM65_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704497.1"
                     /translation="LKDRYGTKIVAVEALECPTLLENGFGEHNIQGIGDKHLPLIHNI
                     MNHDLVAAISDQATDQLAVLFTSEVGKRYLVGKGVPQGVVDQLVDFGLSSICNVLAGI
                     SAAKVLGLGETDVIVTVATDGSQMYPSEIAKVTERDFGGELTELDAAEVWGKHLANVP
                     TSATRELTEKDRNRIFNLGYYTWVEQQGTPFELFQRRRSQDFWTGLRPFLGRWDAMIE
                     DFNAKVAAR"
     gene            681..1916
                     /locus_tag="VHM65_01975"
     CDS             681..1916
                     /locus_tag="VHM65_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HEX2704498.1"
                     /translation="MTAGRRGTVTGYRCATCGTRVAIAAVHPFRCPAAPAGDAHHVLH
                     PVVDGPDPGLVEDPNPFVAYGPSLAWWAFARAHGMSDEQCMSLTRDVANGFAATPFGP
                     HAGLSERLGAEVWVKDETGNVGGSHKARHLVGILLHLRAAEELGLVTGERRPLAIASC
                     GNAAIAAATLAHRVGWPLQVYVPETVSDAIATTLSGLGAEITRCSRTQDRPTGDPAVV
                     AFREAVAAGATPFSVQGPENGLCLDGGRTLGWEMAERLAPPARVVVQVGGGALAACLG
                     WGLGPTIRLDTVQTEGGAPLARAWLRAGSLPTGELGRHWGELMTPWDAPRSLADGILD
                     DETYDWQADVAVMRASGGTPIVVSEDNVVSAYAIATSTGIPVSPTGSAGLAGLLPTPD
                     GPAVVPGERVAIVFSGVRR"
     gene            complement(1923..>2960)
                     /locus_tag="VHM65_01980"
     CDS             complement(1923..>2960)
                     /locus_tag="VHM65_01980"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF006679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HEX2704499.1"
                     /translation="LERRALPEEARLADDGYAAGVAHDFLASLVAAGTTSALIFGSHF
                     PGAVDLLFREAQRVGVRVTSGLVVSDRFLRSDLLTTPENAVEAALRLARTWHGTGRAR
                     YAVMPRFALSCSDDMLASCAAVLAEVPGALFTSHVNENLGEIDQVCASFGGTYVDSYD
                     RHGLVGRHTVLAHNVHPDDGELAVLAERDAAVAHCPSSNCALGSGIFPMRRHLDHGVR
                     FALGTDVGAGTSFSLLREGLQAYFVQRVRGPDGVSLSAADLLYLATAAGADALDLPDV
                     GDLSVGKRFDAVWLRPRPGSTLDVALRHAADTVDALGRVFALGSAQDVAAVWVDGARV
                     SSRMDGSLPGR"
BASE COUNT          428 a         1071 c         1057 g          404 t
ORIGIN      
        1 ctcaaggacc gctacgggac gaagatcgtc gcggtcgagg ctctggagtg cccgaccctc
       61 ctcgagaacg gcttcggtga gcacaacatc cagggcatcg gcgacaagca cctgccgctc
      121 atccacaaca tcatgaacca cgacctcgtc gcggcgatca gcgaccaggc caccgaccag
      181 ctcgcggtgc tcttcacctc cgaggtcggc aagcgctacc tcgtcggcaa gggtgtgccg
      241 cagggcgtcg tcgaccagct cgtcgacttc ggcctgtcct ccatctgcaa cgtcctcgcg
      301 ggcatctcgg cagcgaaggt cctcggcctc ggtgagaccg acgtcatcgt caccgtggcc
      361 accgacggct cgcagatgta cccctccgag atcgcgaagg tcaccgagcg cgacttcggt
      421 ggcgagctca ccgagctcga cgcggccgag gtctggggca agcacctggc caacgtgccg
      481 accagcgcga cgcgagagct caccgagaag gaccgcaacc ggatcttcaa cctcggctac
      541 tacacctggg tcgagcagca gggcacgccc ttcgagctgt tccagcggcg ccggtcgcag
      601 gacttctgga ccggcctgcg gcccttcctc ggccgctggg atgccatgat cgaggacttc
      661 aacgccaagg tcgccgcacg gtgacggcgg gccgtcgcgg caccgtcacc ggctaccgct
      721 gcgccacgtg cggcacacgg gtcgccatcg cagcggtgca cccgttccgc tgccccgccg
      781 cgccggcggg cgacgcccac catgtcctcc atccggtggt cgacggtcca gaccccggtc
      841 tcgtcgagga ccccaacccg ttcgtggcct acggtccgtc gctggcctgg tgggcgttcg
      901 cgcgggccca cggcatgagc gacgagcagt gtatgtcgct gacccgcgac gtcgcgaacg
      961 gcttcgcggc gaccccgttc gggccgcacg cgggcctgtc cgaacggctg ggtgccgagg
     1021 tgtgggtcaa ggacgagacc ggcaatgtcg gcggcagcca caaggcccgg cacctcgtcg
     1081 gcatcctgct ccacctgcgc gcggccgagg agctgggcct ggtcaccggg gagcgccggc
     1141 cgctggccat cgcgtcgtgc ggcaacgccg ccatcgccgc ggccaccttg gcgcaccggg
     1201 tgggctggcc gctgcaggtc tacgttcccg aaacggtgag cgatgccatc gctacgacgt
     1261 tgtccggact gggagccgag atcactcggt gttcccggac gcaggaccgg ccgacgggcg
     1321 accccgccgt ggtcgccttc cgcgaggcgg tggccgcggg agcgactccg ttcagcgtcc
     1381 agggcccgga gaacgggctg tgcctcgacg gcggccggac cctcggctgg gagatggccg
     1441 agcggctcgc tccccccgcc cgggtggtcg tccaggtcgg cgggggcgcg ctggcggcct
     1501 gcctcgggtg ggggctgggt ccgacgatcc ggctcgacac cgtccagacc gaggggggag
     1561 cgccgttggc tcgcgcgtgg ctccgtgccg ggtcgctgcc caccggcgag ctcggacggc
     1621 actggggcga gctgatgacc ccgtgggacg ctccgagatc cctcgcggac ggcatcctcg
     1681 acgacgagac ctacgactgg caggccgatg tcgccgtcat gcgggcctcc gggggcacgc
     1741 ccatcgtggt gtcggaggac aacgtcgtgt cggcatacgc catcgcgacg agcacgggga
     1801 tcccggtaag cccgacgggt tcggccgggc tggccgggct gctgcccacc ccggacggtc
     1861 cggcggtcgt tcctggcgaa cgagtcgcga tcgtgttctc cggcgtgcgc cgctgaccga
     1921 ggctacctgc cggggaggct cccgtccatc cgtgaggaga ccctcgcccc gtcgacccag
     1981 accgcggcga cgtcctgcgc cgacccgagc gcgaacaccc tcccgagggc atcgacggtg
     2041 tcggccgcgt gccgcaaggc gacgtccaag gtgctccctg gccggggccg cagccacacc
     2101 gcgtcgaagc gcttcccgac ggagagatcg ccgacgtcgg gcaggtcgag agcgtcggct
     2161 ccggccgcgg tggcgagata cagcaggtcc gcggcactca ggctgacacc gtcgggcccg
     2221 cgcacccgtt gcacgaaata ggcctgaagc ccctcgcgca gcaggctgaa cgacgtcccg
     2281 gcgcccacgt cggtgcccag ggcgaaccgg acgccgtggt cgaggtggcg ccgcatcggg
     2341 aagatcccgc tgccgagcgc gcagttgctg ctggggcagt gcgcgacggc ggcatcgcgt
     2401 tcggcgagca ccgcgagctc gccgtcgtcc gggtgcacgt tgtgtgcgag cacggtatgg
     2461 cggcccacga gcccgtgccg gtcgtagctg tcgacgtagg tgccgccgaa gctggcgcac
     2521 acctggtcga tctcaccgag gttctcgttg acgtgggagg tgaacagggc tccgggtact
     2581 tccgccagta ccgccgcgca cgacgcgagc atgtcgtcgc tgcacgacag cgcgaaccgc
     2641 ggcatgacgg cataccgtgc ccggcccgtc ccgtgccagg tccgggccag ccgaagggcc
     2701 gcctcgacgg cgttctccgg cgtggtgagc agatcgctgc gcaggaaccg gtcgctgacg
     2761 accagccccg acgtgacccg caccccaacg cgctgggcct cgcggaagag caggtcgacg
     2821 gcgcccggga agtgcgaccc gaagatgagc gcgctcgtcg tgcctgccgc cacgagcgag
     2881 gcgaggaagt cgtgggcgac gccggcggcg tacccgtcgt cggccagccg cgcctcctcg
     2941 gggagcgccc gtcgctcgag
//
LOCUS       DATAIX010000129         2900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_241593, whole genome shotgun sequence.
ACCESSION   DATAIX010000129 DATAIX010000000
VERSION     DATAIX010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241593"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VHM65_01985"
     CDS             complement(<1..312)
                     /locus_tag="VHM65_01985"
                     /inference="COORDINATES: protein motif:HMM:NF015414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704500.1"
                     /translation="MSQTPHQSYLTQEAYDRLTGELEYLQGEGRADISKKIGAAREEG
                     DLRENGGYHAAREEQGKMEARIRQVRDLLDHAVVGETPPDDGVVEPGMIVTVEIFGEE
                     ET"
     gene            complement(1002..1367)
                     /locus_tag="VHM65_01990"
     CDS             complement(1002..1367)
                     /locus_tag="VHM65_01990"
                     /inference="COORDINATES: protein motif:HMM:NF025520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4307 domain-containing protein"
                     /protein_id="HEX2704501.1"
                     /translation="MAIPRPEPGTGKWWAIGIIGCTAGVALISWGQLAGTADRVTGMT
                     VSYRVVDAQNVTVTFDVHKRRDLAAVCTVRALDERFGTVGSLEVAIPPVDAPASNHTV
                     SIRTTSLAVTGEVQKCRGV"
     gene            1492..2451
                     /gene="mca"
                     /locus_tag="VHM65_01995"
     CDS             1492..2451
                     /gene="mca"
                     /locus_tag="VHM65_01995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HEX2704502.1"
                     /translation="MAVRAGLRLMAVHAHPDDESSKGAATTAMYVAQGAEVMVVSCTG
                     GERGDILNLQLREDPRVQRDIAEFRREEMARAAEILGVHHVWLGFVDSGLPEGDPAPP
                     LPDGCFALEPIEVTTEALVRVVREFRPHVMTTYNELGGYPHPDHVNTHTVAMSAFTAA
                     GDPTAYPHAGTPWQPLKIYYDVTFSKARVEALHDAIIASGEESPYAQWLASWTDSRPV
                     RRITTRVACGAYFETRNRALIAHATQVDPDGTWFRVPLDVQRRVWPTEDWEAALSYVP
                     VAGGEDDLFAGLGTAQEADRLALTPGRPIVYDGRVTAAQEESA"
     gene            2448..2678
                     /locus_tag="VHM65_02000"
     CDS             2448..2678
                     /locus_tag="VHM65_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704503.1"
                     /translation="MTFDTTTPTTNIGPGLGAFLSFFVLALALWLLMRNMNARMRRIA
                     YRERAEQEAATGQAADLGDARTAEEPQDPGVA"
     gene            complement(2657..>2900)
                     /locus_tag="VHM65_02005"
     CDS             complement(2657..>2900)
                     /locus_tag="VHM65_02005"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX2704504.1"
                     /translation="GWVAVFYFGPMLRGGGVAVMSLIVAGGVLYTLGAVVYATKRPDP
                     SPRWFGFHEVFHACTVLAFAAHYIAVSMAVYATPGS"
BASE COUNT          426 a          988 c         1057 g          429 t
ORIGIN      
        1 ggtctcctcc tcgccgaaga tctcgacggt gacgatcatg ccgggctcga cgacaccgtc
       61 gtccggcggg gtctcgccga cgacggcgtg atcgagcagg tcacgaacct ggcggatccg
      121 ggcctccatc ttgccctgct cctcgcgggc cgcatggtag ccgccgttct cgcgcaggtc
      181 accctcctcg cgggcggccc cgatcttctt ggagatgtcg gcgcggccct caccctggag
      241 gtactcgagc tcgccagtga ggcggtcgta cgcctcctgc gtcaggtagc tctggtgtgg
      301 ggtctgcgac atggtcagct ccttgggtgg tccggcgacg aaggctcagg ccggatccgg
      361 accggtccgc gaggcgcgaa gcgctgcggg ccggccgttc tgggcaagac tccaggctac
      421 caaagcctca gagcgcgtcg ggagctcggg tggccgggtt tggggcggcc aggcggcggg
      481 acatgatcgc gatgtagatc atggtctcgt ggtcctcggg ctggttctcc gtggtcgcgg
      541 cggcggtgtg tggtggtccg gccgaaggtg cgttgtccga ccgggcgtcg gggtgcgacg
      601 acgagcccgg tggtgtcgtc ggtgtgtttg gcgagcgtca tgtccaggga gagaacggtt
      661 tggctcactg caccggtcgg ccgcggtatc cgccatccgg ccgcggtatc cgccatccgg
      721 ccaagaccaa gtcgaggctg atcgcgggcg ggagctccgc gtatagctgg cagccgtggg
      781 ttagcgcggt ggatctggcc ggtgctggtc ggccggatcg gcctcgacga gccagccctg
      841 gacgaccgcg gccgaacaaa cgggcgggaa cgggccaggg ggcagcatca cgctgccccc
      901 tggtgggcgc ggtctcggca accaatgacc taccaggggc tcacctaccc accgcaacac
      961 aggcatcacg cgcccgccag caaacagggt tcccaggcgc cctagacgcc ccggcatttc
     1021 tggacttcac cggtcacggc caggctcgtc gtgcggatcg acacggtgtg gttgctcgcc
     1081 ggcgcgtcga cgggcgggat cgccacctcg agcgagccga ccgtgccgaa gcgctcgtcg
     1141 agggcacgca ccgtgcacac tgccgcgagg tcgcggcgct tgtggacgtc gaaggtcacc
     1201 gtgacgttct gggcgtcgac gacccggtag ctcaccgtca taccggtcac gcggtccgcg
     1261 gttcccgcca gctgccccca ggagatgagg gcgacaccgg ccgtgcaccc gatgatgccg
     1321 atggcccacc acttgccggt gcctggttcg ggtctgggga tggccacggg atgagctcct
     1381 ggtcgtgctc ggcccgctcg cgcggggtga catgatgggt cactgtccat tgtcgagcac
     1441 cgcaccggtg ggccgtgaca gcacggcccg gtcaggcagg agagcacagg tatggcggtc
     1501 cgtgcgggtc tgcggctcat ggcggtccac gcacacccgg acgacgagtc gagcaagggc
     1561 gcggcgacga cggcgatgta cgtcgcccag ggtgccgagg tcatggtcgt ctcgtgtacc
     1621 ggtggtgagc ggggggacat tctcaacctg cagctgcgtg aggacccccg cgtccagcgt
     1681 gacatcgcgg agttccggcg cgaggagatg gccagggcgg ccgagattct cggcgtccac
     1741 cacgtctggc tcggtttcgt cgactcgggg ctgcccgagg gcgacccggc tccgcccctg
     1801 cctgacggct gcttcgccct cgaaccgatc gaggtgacga ccgaggcgct cgtgcgggtg
     1861 gtccgcgagt tccgcccgca cgtcatgacg acctacaacg agctgggcgg ctacccgcac
     1921 ccggaccacg tcaacaccca caccgtggcg atgtctgcct tcaccgcggc cggggacccc
     1981 acggcatacc cgcacgcggg cacaccgtgg cagccgctca agatctacta cgacgtcacc
     2041 ttctccaagg cgcgggtcga ggccctgcac gacgcgatca tcgccagtgg cgaggagtcg
     2101 ccctacgcgc agtggctggc gagctggacc gactcgcgcc cggtgcggcg catcacgacg
     2161 cgggtcgcgt gcggtgccta cttcgagacc cgcaaccggg ccctcatcgc ccacgcgacc
     2221 caggtcgacc cggacggcac gtggttccgg gtccccctgg acgtgcagcg gcgggtctgg
     2281 ccgaccgagg actgggaggc ggcgctgtcc tacgtgcccg tcgccggcgg cgaggacgac
     2341 ctgttcgcag ggctcgggac ggcgcaggag gccgaccggc tcgccctgac gcccggacgc
     2401 ccgatcgtgt atgacggtcg cgtcaccgcc gcgcaggagg agtccgcatg actttcgaca
     2461 cgacgacacc gacgacgaac atcggcccgg ggctgggggc gttcctgtcg ttcttcgtgc
     2521 tggcgctggc gctctggctg ctcatgcgca acatgaacgc gcggatgcgc cggatcgcct
     2581 accgcgagcg ggcggagcag gaggcggcca ccgggcaggc ggccgacctg ggcgacgctc
     2641 ggacggcgga ggagcctcag gaccccgggg tcgcgtagac cgccatcgag accgcgatgt
     2701 agtgggcggc gaaggcgagc accgtgcacg cgtggaacac ctcgtggaaa ccgaaccagc
     2761 gcggggaggg gtcgggccgc ttcgtggcgt agaccaccgc cccgagggtg tagagcaccc
     2821 cgccggccac gatgaggctc atgacggcca ccccgccgcc gcgcagcatc ggcccgaagt
     2881 agaagacggc gacccagccc
//
LOCUS       DATAIX010000130         2790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_67139, whole genome shotgun sequence.
ACCESSION   DATAIX010000130 DATAIX010000000
VERSION     DATAIX010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67139"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VHM65_02010"
     CDS             <1..243
                     /locus_tag="VHM65_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704505.1"
                     /translation="IPGLDGSRVTFDVTYNPATQKYAGAVDISGAGFDAHIRVQAGRF
                     GVTQNGDMVKGTLYGIKALSNPKRCFVIAFRIRDLG"
     gene            complement(374..1006)
                     /locus_tag="VHM65_02015"
     CDS             complement(374..1006)
                     /locus_tag="VHM65_02015"
                     /inference="COORDINATES: protein motif:HMM:NF037625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX2704506.1"
                     /translation="MHERAHRGGLRERKRVSAMRHIQQVAIDLFDERGFDAVTIEAVA
                     AAAEVSPSSVYRYFGTKEGLVVRDDADRTLVASFAAHLVEHDVWDAVDLAIEDADPAG
                     FEPVAALHLARMRLFHDVPSVRGRLLVEAADAIDELAGVLMSPTAKVPRSRFEARVVA
                     TALVGALVVAAQEWYAAGGAAPILDLARTSIDLVRTDRQAGRGDRPGQGS"
     gene            1062..2039
                     /locus_tag="VHM65_02020"
     CDS             1062..2039
                     /locus_tag="VHM65_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2704507.1"
                     /translation="MRKSLSFSRRTTPTSDQVIDAAGLAKRFGRVTALDGLDLQVRRH
                     EVHGFLGPNGSGKSTTIRALLGQLRLDGGHAQVLGQDPWRDAVAIHERLAYVPGDVTL
                     WPGLTGGECIDVLGRFQGRANGARRDELVERFALDPSRKTRTYSKGNRQKVALVAALA
                     TDVELLLLDEPTAGLDPLMEAEFQTCIRETMGQGRTVLLSSHILGEVEALCDRVSIIR
                     RGRTVRNGTLEELRAETLTTVEATLRHPIPDLAGAPGVESWHEHTLPDGATRATVRVR
                     AEALTPVVSLLIAYQASGLVVAPPTLDELFLGQYHDAPEPAAAGGGEPV"
     gene            2036..>2790
                     /locus_tag="VHM65_02025"
     CDS             2036..>2790
                     /locus_tag="VHM65_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704508.1"
                     /translation="MTAGRHESPRWGAWQGSLRLTRLHLRTHRWSVLGWPAVIVALSA
                     STAAGIVSLYATTAARQGYAATIGTSTMSIAFNGRGYDLDTLGGITVNELGVYALLVY
                     PAIAVHLAVRHTRGEEEAGREELLTAARVGRLAPLTAGALVTGLVLVAAGLLSGVSMA
                     AAGLPLGGSMRYATALTLAMLTYAGAGLVAAQLSQSSRVANAVAVGVLGAAYLARAVA
                     DGRGSPLVWPNPTGWLAEAAPFGAARSWPLVGYG"
BASE COUNT          391 a         1015 c         1002 g          382 t
ORIGIN      
        1 atcccagggc tggacggatc cagggtgacg ttcgacgtca cctacaaccc ggcgacgcag
       61 aagtacgcgg gagccgtcga catctctgga gccgggttcg acgcgcacat ccgcgtgcag
      121 gctggccggt tcggcgtcac ccagaacggc gacatggtca agggcacgct ctacgggatc
      181 aaagccctga gcaacccgaa gaggtgcttc gtgatcgcct tccggatcag ggacctgggc
      241 tgaccctggg gtgaccgtgc gggagggcct gcgggacgga cgcgaccacc ggtccgagac
      301 gccgtctgca cagttgcgag cacagcgccc gtcctgagcg gggatggtga tgtccttcgg
      361 ggatggtgac gtcctacgaa ccctgaccgg gcctgtctcc tcggccagcc tgccggtcgg
      421 tgcggacgag gtcgatgctc gtccgggcca ggtcgaggat cggcgcggcg ccccccgcgg
      481 cataccactc ctgcgccgcg acgacgagcg caccgacgag cgcggtggcc acgacgcggg
      541 cctcgaagcg ggagcgcggg accttcgccg tgggggacat gaggaccccg gccagctcgt
      601 cgatggcgtc ggcggcctcg acaagcaggc gcccgcggac gctgggcacg tcgtggaaga
      661 gcctcatccg ggccagatgg agcgcggcga ccggctcgaa gccggccggg tcggcatcct
      721 cgatggccag gtcgacggcg tcccagacat cgtgctcgac gaggtgggcc gcgaacgagg
      781 ccaccagggt gcggtccgcg tcatcgcgga cgacgaggcc ctccttggtg ccgaagtaac
      841 ggtagaccga gctcggcgac acctcggcgg ccgccgcgac cgcctcgatg gtcaccgcgt
      901 cgaaaccgcg ctcgtcgaac aggtcgatgg ccacctgctg gatgtggcgc atggcgctga
      961 cgcgcttgcg ctcacgcagc ccgccacggt gcgcccgctc gtgcatgtga cctcgccttc
     1021 tcccggctcg ctccgatggt atgccgagta gagttccgtg catgagaaag tcactgtcat
     1081 tttctcggag gacgacgccc acctcggacc aggtcatcga cgcggccggc ctggccaaac
     1141 gcttcggccg ggtgaccgct ctcgacgggc tggacctgca ggtgcgccgt catgaggtgc
     1201 atggcttcct cggacccaac gggtcgggca agtcgacgac gatccgggcc ctcctgggcc
     1261 agctgcgcct cgacggcggg cacgcgcagg tcctcgggca ggatccgtgg cgggacgcgg
     1321 tcgccatcca cgagcgcctc gcctacgtgc cgggggacgt cacgctgtgg ccgggcctga
     1381 ccggtggcga gtgcatcgac gtcctcggcc ggttccaggg ccgggcgaac ggggcccggc
     1441 gcgacgagct cgtcgagcgc ttcgccctcg acccgagccg caagacccgc acgtactcca
     1501 agggcaaccg ccagaaggtc gccctcgtcg ccgcgctcgc gaccgacgtc gagctgctgc
     1561 tgctcgacga gccgacggcc ggtctggacc cgctcatgga ggccgagttc cagacctgca
     1621 tccgcgagac gatgggccag ggccgcacgg tgctgctcag cagccacatc ctcggcgagg
     1681 tcgaggccct gtgcgaccgc gtgagcatta tccggcgagg ccggaccgtg cgaaatggca
     1741 ccctcgagga gctgcgcgcc gagacgctca cgacggtcga ggcgacgctg cgccatccga
     1801 tccccgacct cgccggggcg ccaggcgtcg agagctggca cgagcacacc ctgcccgacg
     1861 gggccacccg cgccaccgtc cgcgtccggg ccgaggcgct cacccccgtg gtgtccctgc
     1921 tcatcgccta ccaggcgagc gggctggtcg tcgctccgcc gacgctggac gagctcttcc
     1981 tcggccagta ccacgacgcc cccgagcccg ccgcagccgg tggaggtgaa ccggtatgac
     2041 ggcgggtcgc cacgagtcgc cccgctgggg ggcctggcag ggcagcctgc gcctgacccg
     2101 gctgcacctg cgcacccacc ggtggagcgt gctcggctgg ccggcggtga tcgtcgcact
     2161 gtcggcatcg acggccgcgg gcatcgtgag cctgtacgcc acgaccgccg cgcggcaggg
     2221 gtatgccgcg accatcggca cgtcgacgat gtcgatcgcc ttcaacggcc gggggtacga
     2281 cctggacacc ctcgggggca tcaccgtcaa cgagctcggc gtctacgccc tgctcgtcta
     2341 cccggccatc gcggtccacc tcgcggtccg ccatacccgc ggggaagaag aggccgggcg
     2401 cgaggagctg ctcaccgcag cccgcgtggg gcgcctggcc ccgctcactg ccggagccct
     2461 tgtcaccggg ctcgtgctcg tggcggccgg cctgctcagc ggcgtgtcga tggccgcggc
     2521 ggggctcccg ctcggcgggt cgatgcgcta cgccaccgcg ctgaccctgg cgatgctcac
     2581 ctatgccggg gccgggctgg tcgccgccca gctcagccag agcagccggg tcgccaacgc
     2641 ggtcgccgtc ggggtcctcg gcgccgccta ccttgcccga gccgtcgccg acggccgggg
     2701 ctcgccgctg gtctggccca acccgacggg gtggctcgcc gaggccgctc cgttcggcgc
     2761 ggcccggagc tggcccctcg tggggtatgg
//
LOCUS       DATAIX010000131         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_96805, whole genome shotgun sequence.
ACCESSION   DATAIX010000131 DATAIX010000000
VERSION     DATAIX010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96805"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VHM65_02030"
     CDS             <1..241
                     /locus_tag="VHM65_02030"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX2704509.1"
                     /translation="VPQDRDGFSRALREEYQVGSGMFYPVPNHRLAPFRVSADLPETE
                     RAAATCLSLPVHPSLCQGDLERIVAAVNALARAGA"
     gene            243..1229
                     /locus_tag="VHM65_02035"
     CDS             243..1229
                     /locus_tag="VHM65_02035"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2704510.1"
                     /translation="MANLRAGLIGLGMMGRHHARVMSSLPGVDLVAVADPGGDPYGVA
                     GGRPLLASVEDLLAAGIDYCLVAVPTVHHEVLGLALAAAGVHAMIEKPLAGDTAAAAR
                     VAGAFDANGLVGAVGHIERYNPALQSARARIEAGELGQVFQITTRRQGPFPARIADVG
                     VVKDLATHDIDLTAWVAQQHYTAVAARTAYRSGRVHEDLVAVTGALADGTVTNHLVNW
                     LSPLKERVTVITGEKGAFVADTLTADLTYYANGQIETTWDEIRNFRGVSEGDIVRFAI
                     AKPEPLRVEHENMRDAVLGLDADIVTMAQGLTTVRVAEAVLESARSGQTVQV"
     gene            complement(1257..2324)
                     /gene="wecB"
                     /locus_tag="VHM65_02040"
     CDS             complement(1257..2324)
                     /gene="wecB"
                     /locus_tag="VHM65_02040"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX2704511.1"
                     /translation="MKVLSVVGARPQFVKLAPIAAQLSLEGHEHVIVHTGQHYDALLS
                     DVFFADLSIPAPDVHLGVGSGSHGVQTGSMLAAMDAVFEEHHPDWVLVYGDTNSTLAG
                     AVSAVKLHLPLAHLEAGLRSFNRRMPEEHNRVLTDHAADLCLAPTQVAMDHLADEGLA
                     RRAVLVGDVMTDVCFRVRAAMAARAPELPDGVDPAQPYLVATIHRADNTDDPDRLRAV
                     IEALADLPVPVVLLAHPRLVSRAASFGVDLRAGALHPGAPLPYPQMVRAVESAAGVVT
                     DSGGLQKEAFLLGTPCTTLRTETEWTETLVDGWNILDPGLTQVRQVAVRARPTQPRAT
                     PYGDGTAARAAVDALARSVRT"
BASE COUNT          354 a          832 c          866 g          328 t
ORIGIN      
        1 ggtcccgcag gaccgcgacg ggttctcccg ggcgctgcgc gaggagtacc aggtcggctc
       61 ggggatgttc taccccgtcc cgaaccaccg cctggctccg ttccgggtga gcgcggatct
      121 gccggagacc gaacgggccg cggccacgtg cctctcgctc cccgttcacc cctcgctgtg
      181 ccagggcgat ctcgagcgga tcgtggccgc ggtcaacgcc ctcgcgcgtg cgggagcctg
      241 acatggccaa cctcagggcc ggcctcatcg gcctcggcat gatggggcgc caccatgccc
      301 gggtcatgag cagtctgccc ggcgtcgacc tggtggcggt ggccgacccg ggcggtgacc
      361 cctatggggt ggcgggcgga cgtccactgc tcgcctcggt cgaggacctc cttgcggcgg
      421 gtatcgacta ctgcctcgtg gcggtcccga ccgtccacca cgaggtgctc gggctggcgc
      481 tcgccgcggc cggggtgcac gccatgatcg agaagccgct ggcaggggac accgcggccg
      541 ccgcccgcgt ggccggggcg ttcgacgcca acgggctcgt cggcgcagtc ggccacatcg
      601 agcgctacaa ccccgccctg cagtcggcgc gggcgcgtat cgaagccggc gagctggggc
      661 aggtcttcca gatcacgacc cggcgccaag ggcccttccc ggcccggatc gccgatgtcg
      721 gcgtcgtcaa ggacctggcc acgcacgaca tcgacctgac ggcctgggtc gcgcagcagc
      781 actacaccgc cgtggcggcg aggacggcat accgctcggg gcgggtgcac gaagacctcg
      841 tcgcggtcac cggggccctg gccgacggga cggtgaccaa ccatctcgtc aactggctct
      901 cgcccctcaa ggagcgggtc accgtcatca ccggcgagaa gggcgcgttc gtggcggaca
      961 cgctgaccgc cgacctcacg tactacgcca acggccagat cgagaccacc tgggacgaga
     1021 tccgcaactt ccggggggtc tccgagggcg acatcgtccg gtttgccatt gccaagccgg
     1081 agccactgcg cgtcgagcac gagaacatgc gcgacgccgt gctgggcctc gacgcggaca
     1141 tcgtcacgat ggcgcagggg ctcacgacgg tgcgggtcgc cgaggccgtg ctcgagtcgg
     1201 cacgcagcgg tcagaccgtc caggtctgac gcccgcgggc acggcgggcg ggccggtcag
     1261 gtccgcacgg accgggcgag ggcatcgacg gcggcccgcg ccgccgttcc gtccccgtac
     1321 ggcgtggccc gcggctgggt gggacgcgca cgcacggcca cctggcgaac ctgagtcagc
     1381 cccgggtcga ggatgttcca gccgtcgacg agcgtctcgg tccactcggt ctcggtgcgc
     1441 aacgtcgtgc acggggtgcc gagcaggaag gcctccttct gcagtccccc cgagtcggtc
     1501 accacaccag cggcgctctc gaccgcgcga accatctgcg ggtagggaag cggcgcgccc
     1561 gggtgcaggg cccctgccct gaggtcgact ccgaacgacg cggcccggct caccagtctc
     1621 ggatgcgcga gcaggacgac gggaaccggc aggtctgcca gcgcctcgat caccgctcga
     1681 aggcggtccg ggtcgtcggt gttgtcggcg cggtggatcg tcgcgacgag gtacggctgg
     1741 gccgggtcga caccgtccgg gagctcggga gcgcgggccg ccatcgcggc acgcacccgg
     1801 aagcagacgt cggtcatgac atcaccgacg aggactgctc gacgagcaag tccctcatcg
     1861 gcaaggtggt ccatggccac ttgagttggc gcgaggcaca ggtcggcggc atggtcggtg
     1921 aggacgcggt tgtgctcttc cggcatgcgc cggttgaagg agcgcaggcc cgcctcgaga
     1981 tgcgccaggg gaaggtgcag cttgaccgcc gacaccgcac cggccagcgt cgagttcgtg
     2041 tcgccgtaga cgagaaccca gtccgggtgg tgctcctcga acacggcgtc catggccgcg
     2101 agcatgcttc ccgtctggac tccatgcgag cccgagccga cgccgaggtg gacgtccgga
     2161 gccggaatgg acaggtcggc gaagaagacg tcggacagga gcgcgtcgta gtgctgcccg
     2221 gtgtggacga tgacgtgctc atgcccctcg aggctgagct gggccgcgat cggggcgagc
     2281 ttgacgaact gcggacgggc cccgaccacg ctgagcacct tcacgagctg gttccttccg
     2341 gggggaggcg gtcgatcacg tgccccaccc accctagagc
//
LOCUS       DATAIX010000132         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_271426, whole genome shotgun sequence.
ACCESSION   DATAIX010000132 DATAIX010000000
VERSION     DATAIX010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271426"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="VHM65_02045"
     CDS             complement(<1..1225)
                     /locus_tag="VHM65_02045"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX2704512.1"
                     /translation="MSRAARVGRPGSLGPTRGEREGPLGAVAIATALGNPPPTDEQRA
                     IIEADPSRPLLVVAGAGSGKTETMASRVVWLVANGHVDPDQVLGLTFTRKAATELAER
                     IVRRLRTLQATGLWRPRTGDGSGAEALLAMPTVSTYHSYAGRLVREHALRLGIEPESR
                     LLSEAAAWQYAAEVVARYDGPMEDVSSAESTVIDAVVSLAGELAEHLLAPADVEAYLA
                     QFEARMAALPKGPSKAHSVPIGDTLKALRGRRAILPIVSRYLDLKRSRDAVDFADQMA
                     LAARLARDFAGVGAIERERFGAVLLDEFQDTSEAQLVLLRSLFVAPVGATAVTAVGDP
                     NQSIYGWRGASATTLARFPQEFADRQGPARVVALSTSWRNDEAILAAANATSAPLRTG
                     HVGVLRARSGAGPGAH"
     gene            complement(1249..>2079)
                     /locus_tag="VHM65_02050"
     CDS             complement(1249..>2079)
                     /locus_tag="VHM65_02050"
                     /inference="COORDINATES: protein motif:HMM:NF022379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX2704513.1"
                     /translation="VPGADPARWWVQTALSDDRPLRAPDQHVRVSPSKVESFTQCRLR
                     WLLTSTGGDGPGVGAATIGALVHDIAHEMGDADAATLVQAVHARWGRLGMTPGWVSDR
                     KRAEALDMVGRLAAYVEQARGQGWAKVGSELPVSVQVGRAEVTGRVDRLERDPEGRLR
                     AIDYKTGSKHFNDAAVQEHPQLATYQVAIERGAFKELGSTSAGAALVQLGKAATRANP
                     PLSQRPLAGHDDPQWAERLVAGVAEGMGGGQFAATQGEWCKLCPVKGSCPAQPDGEGI
                     "
BASE COUNT          275 a          775 c          755 g          274 t
ORIGIN      
        1 ggtgtgctcc gggcccggct cccgaccggg cccggagcac acccacatga cccgtccgca
       61 agggggccga ggtcgcgttg gccgccgcga ggatggcctc gtcgttgcgc cagctcgtgc
      121 tgagcgcgac aactcgcgcc ggcccttgcc ggtcagcgaa ctcctgcggg aaccgggcca
      181 gcgtcgtcgc gctcgcgccg cgccagccgt agatcgactg gttcgggtcg ccgacggcgg
      241 tcaccgcggt ggcgcccaca ggagccacga agagcgaacg cagcagcacg agctgggcct
      301 cggaggtgtc ctggaactcg tcgagcagga ccgcgccgaa ccgctcacgc tcgatggccc
      361 cgaccccggc gaagtcccgg gccagccgag cggccagcgc catctggtcg gcgaagtcga
      421 cggcgtcccg gcttcgcttg aggtcgagat agcgcgacac gatgggtagg atcgcgcggc
      481 gtccgcgcag cgctttgagg gtgtccccga tcggcaccga gtgcgccttc gacggcccct
      541 tgggcagcgc ggccatccgc gcctcgaact gcgccaggta ggcctcgacg tcggcgggag
      601 ccagcaggtg ctcggcgagc tctccggcca gcgagaccac ggcgtcgatg acggtcgact
      661 cggcactgct gacgtcctcc atggggccgt cgtagcgggc gaccacctcc gcggcatact
      721 gccacgccgc cgcctcggag agtagccgcg actccggctc gatcccgagc cgcagggcgt
      781 gctcgcggac aaggcgaccg gcatacgagt gataggtcga caccgtcggc atggcgagaa
      841 gcgcctcggc gcccgacccg tcgccggtcc gcggccgcca cagcccggtg gcttgcaggg
      901 tgcgcaggcg ccggacgatg cgctcggcga gctcggtggc cgccttgcgg gtgaaggtga
      961 gaccgaggac ctggtcgggg tcgacatggc cgttggcgac gagccagacg acgcgtgagg
     1021 ccatcgtctc ggtcttgccc gagccggcac cggcgacgac gagtagcggc cgcgacgggt
     1081 cggcctcgat gatcgcccgc tgctcgtcgg tcggtggtgg gttgccgaga gcagtggcga
     1141 tcgcgacggc accgagaggg ccctcccgct ccccacgggt cgggccgagg gatcccggcc
     1201 gccccacgcg ggccgcgcga ctcacggcag acccgtcaac cggatgcatc agatgccctc
     1261 accgtcgggc tgggccgggc aggacccctt gaccgggcag agcttgcacc actcgccctg
     1321 ggtcgccgcg aactgcccgc cgcccatccc ctcggcgacc cccgcgacga gccgctcggc
     1381 ccactgcggg tcgtcgtgcc cggcaagcgg gcgctgggac agcggtgggt tggcccgggt
     1441 cgcggcctta ccgagctgga cgagggccgc cccggccgag gtggacccga gctctttgaa
     1501 cgcccctcgc tcgatggcca cctggtaggt cgccagctgc ggatgctcct gcaccgccgc
     1561 gtcgttgaag tgcttcgacc ccgtcttgta gtcgatcgcc cggagccggc cctcggggtc
     1621 tcgctcgagt cggtcgaccc ggccggtgac ctcggcccgg ccgacctgca cgctgaccgg
     1681 gagctcgctg ccgaccttgg cccagccctg cccgcgcgcc tgctcgacat acgccgcgag
     1741 ccgcccgacc atgtcgagcg cctcggcgcg cttgcggtcg ctgacccagc ccggcgtcat
     1801 accgagccgg ccccagcggg cgtgcacggc ctgaaccaga gtcgcggcgt cggcgtctcc
     1861 catctcgtgg gcgatgtcgt ggacgagggc gccgatcgtc gcggcaccga ctcctgggcc
     1921 gtcaccgccg gtgctcgtca ggagccagcg caggcggcac tgcgtgaacg actcgacctt
     1981 ggacggcgac acccggacgt gctggtccgg cgcccgcagc ggccggtcgt cgctcagcgc
     2041 ggtctggacc caccagcggg ccgggtcggc accgggcac
//
LOCUS       DATAIX010000133         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_156729, whole genome shotgun sequence.
ACCESSION   DATAIX010000133 DATAIX010000000
VERSION     DATAIX010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156729"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..497)
                     /locus_tag="VHM65_02055"
     CDS             complement(84..497)
                     /locus_tag="VHM65_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX2704514.1"
                     /translation="MDDLYRDEILEHYRRPHNFGTLQHPDVSYEGANPLCGDRLTMML
                     DLDASGTVTDVAFTGRGCAISQASASMLTDMLRGRSTEELGKLAKDDVLELLGIEISP
                     ARLKCALLSLDTLQRALAERALGGAATPTPAEVRV"
     gene            complement(490..>1610)
                     /locus_tag="VHM65_02060"
     CDS             complement(490..>1610)
                     /locus_tag="VHM65_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX2704515.1"
                     /translation="AEEATAAYEAARASAARFLNAPSSSEIVFVRNATEAINLVAYSW
                     GRRNIRQADTIVLTELEHHSNLVPWQLLVQEQDADLEFVAIDDQGRLDQQSFEVLLRT
                     RPKLVAFNHVSNALGTINPVREMTAQAHAAGALVLIDGAQGVPHAPVDVQDIGADFYV
                     FSGHKALGPTGSGVLWARRELLDAMPPFMGGGEMIREVHLRHTTFNDVPWKFEAGTPD
                     IAAAIGLGAALEYLSVLGMDRVREHERMLTAYALDMLPREVPGIRIIGPLSPAERSGI
                     VTFTLPGIHAHDVATLLDREAIAIRAGHHCTQPLHERLGESATGRASFNVYTTIDDLD
                     RLAAGLRSVQRIFGADTAPGARAAHGEPADRYPVTSHG"
BASE COUNT          253 a          547 c          570 g          240 t
ORIGIN      
        1 ggtctcgcgc cggggtccgg gcggacagat caggctgggt cgggatgccg gtcaggccgg
       61 ccaggtacag tcgcccatgg tgctcacacc cggacctcgg ccggggtcgg ggtcgcggca
      121 ccgccgagcg cacgctccgc cagtgcccgc tggagcgtat ccagcgacag cagggcgcac
      181 ttgaggcggg ccgggctgat ctcgatgccc agcagctcca gcacgtcgtc cttggcgagc
      241 ttgcccagct cctccgtgga ccggccgcgc agcatgtcgg tgagcatcga ggcggatgcc
      301 tggctgatgg cacagccacg acccgtgaag gcgacatccg tgaccgtgcc cgacgcatcc
      361 aggtcgagca tcatggtcag ccggtcgccg cacagcggat tggcgccctc gtacgagaca
      421 tccgggtgct ggagcgtgcc gaagttgtgc ggccgccggt agtgctccag gatctcgtcg
      481 cggtacaggt catccatggg acgtcacagg gtaccggtca gcaggctccc cgtgcgcggc
      541 gcgcgccccg ggagccgtgt cggcgccgaa gatgcgttgc acggaacgga gcccggcggc
      601 gagccggtcc aggtcatcga tggtcgtgta gacgttgaag ctggcccgcc cggtcgccga
      661 ctcgcccagg cgctcgtgga gcggctgggt gcaatggtga ccggcgcgga tggcgatggc
      721 ctcgcggtcc agcagggtcg ccacgtcgtg ggcgtggatg cccggcaagg tgaacgtcac
      781 gatgcccgat cgctccgccg gggacagcgg tccgatgatc cggatgcccg gcacctcccg
      841 gggcagcatg tccagggcgt acgcggtgag catccgctca tgctcgcgca cacggtccat
      901 gcccagcacg gacaggtatt ccagcgcagc gcccagcccg atggccgccg cgatgtcggg
      961 cgtgccggcc tcgaacttcc agggcacgtc attgaacgtc gtgtgccgga gatggacctc
     1021 gcggatcatc tcgccaccac ccatgaacgg cggcatcgcg tccagcagct cccggcgcgc
     1081 ccagaggacc cccgacccgg tcgggccaag cgccttgtga ccggagaaca cgtagaagtc
     1141 ggcaccgatg tcctggacat cgacgggtgc atgcggcaca ccctgcgcac catcgatgag
     1201 cacgagggca cccgcggcat gggcctgcgc ggtcatctcc cgcaccggat tgatggtccc
     1261 aagtgcattc gagacatgat tgaaggcgac cagcttgggg cgggtccgga gcagcacctc
     1321 gaacgactgc tgatcgaggc gcccctgatc gtcgatggcc acgaactcca ggtccgcgtc
     1381 ctgctcctgg acgagcagct gccagggcac caggttggag tggtgctcca gctccgtgag
     1441 cacgatcgtg tccgcctgcc ggatgttccg gcgaccccac gagtaggcga ccaggttgat
     1501 ggcctcggtc gcattgcgca cgaacacgat ctcggagctc gagggtgcgt tcaggaagcg
     1561 cgccgcggac gcacgcgcgg cctcgtacgc ggccgtggcc tcttccgcgg
//
LOCUS       DATAIX010000134         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_37490, whole genome shotgun sequence.
ACCESSION   DATAIX010000134 DATAIX010000000
VERSION     DATAIX010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37490"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VHM65_02065"
     CDS             complement(<1..879)
                     /locus_tag="VHM65_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2704516.1"
                     /translation="MTTQRRPPIGPLPSIATRPLGHPSYRLIAGAGPVQWVCRRPDRE
                     ITMTSEHDTTDLTTTDLTTTGPAGLSDPTGTQDTDAPQATESPAVPELTFADFDVHPD
                     IVADLADAGIIHPFPIQAMTLPVALGGHDIIGQAKTGTGKTLGFAIPALQRAITPNDE
                     AYADLPHAGKPQALMVAPTRELAVQVAGDITAAGRRRGARVLTVYGGRAYEPQIEALR
                     RGVEIVVGTPGRLIDLAKQGHLDLSHAKTVVLDEADEMLDLGFLPDVETLMSLTSANR
                     QTMLFSATMPGAIVALA"
     gene            1050..1763
                     /locus_tag="VHM65_02070"
     CDS             1050..1763
                     /locus_tag="VHM65_02070"
                     /inference="COORDINATES: protein motif:HMM:NF025166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like fold-containing protein"
                     /protein_id="HEX2704517.1"
                     /translation="MDEPSDLTAPAADGSAPATPGLEDAVVDLLGALGYGELMAFSQL
                     AADAEIAPDQSSKVALARLAVAEFHHFELILARITELGGDPDTVMAPFVHSFDDFHAR
                     TRPSGWLEGLVKFYVGDGIASDFYSEIAAYVDPVTRELIHTVMEGKGQDDVIVAMVRQ
                     AISDDTKLAGRLALWGRRLVGEALTQAQRVVVERESVAGLLVGSFGGAGADLAELGRM
                     FARLTDEHTRRMARLGLSA"
BASE COUNT          263 a          692 c          746 g          310 t
ORIGIN      
        1 ggccagcgcg acgatggccc cgggcatcgt cgccgagaag agcatcgtct ggcggttggc
       61 cgaggtgagc gacatgaggg tctcgacgtc gggcaggaag ccgaggtcga gcatctcgtc
      121 ggcctcgtcg agaacgacgg tcttggcgtg gctgaggtcg aggtggccct gcttggccag
      181 gtcgatgagc cgcccggggg tgcccacgac gatctcgacg ccgcggcgca gcgcctcgat
      241 ctgaggctcg tacgcgcgac cgccatacac ggtgaggacg cgcgctccgc ggcggcgccc
      301 cgccgcggtg atgtccccgg cgacctgcac ggcgagctca cgcgtcgggg cgaccatgag
      361 cgcctgcggc ttgcccgcgt ggggcaggtc ggcgtaggcc tcgtcgttgg gggtgatggc
      421 gcgctgcagc gcggggatcg caaagccgag ggtcttgccc gtgccggtct tggcctgccc
      481 gatgatgtcg tgcccgccga gggcgaccgg cagggtcatg gcctggatcg ggaaggggtg
      541 gatgatcccg gcgtcggcga ggtccgcaac gatgtccggg tggacgtcga agtcggcgaa
      601 cgtcagctcc gggaccgccg gggactccgt ggcctggggg gcgtcggtgt cctgggtgcc
      661 cgtcgggtcg ctgaggccgg cggggccggt ggtggtcagg tcggtggtgg tcaggtcggt
      721 ggtgtcgtgc tcggaagtca tggtgatctc tcggtcgggc ctgcggcaca cccactgcac
      781 tggccccgcc ccggcgatga gccggtatga cgggtggccg agcgggcgcg tcgcgatgct
      841 gggaagcggg ccgatcggag gtcttcgttg ggtggtcatg cgaccgtgca tgacgcggat
      901 tcgggccgac cgggcaccgg ataaccccct tagtctaccg gccgggccga ggcgcccacg
      961 ccatgcgcgc acaaccggcc tgcgtcggcc cgcgcagccc ggccggccac cccctgcctc
     1021 ctggtcctgc ggatcgctac tctgacgcca tggacgaacc cagcgacctc actgcccccg
     1081 ccgcggacgg ctcagcaccg gccaccccgg ggctcgagga cgcggtcgtc gacctgctcg
     1141 gtgccctcgg ctatggcgag ctcatggcct tctcccagct cgctgccgac gccgagatcg
     1201 cacccgacca gtcctcgaag gtggccctgg cccgactggc agtcgcggag ttccaccact
     1261 tcgagctgat cctcgccaga atcaccgagc tcggtggcga cccggacacc gtcatggccc
     1321 cgttcgtgca ctcgttcgac gacttccacg cccggacccg tccctcgggc tggctcgagg
     1381 gactggtcaa gttctatgtc ggcgacggca ttgcctcgga cttctactcc gagatcgccg
     1441 cgtatgtcga cccggtgacc cgcgagctca tccacaccgt gatggagggc aagggccagg
     1501 acgacgtcat cgtcgccatg gtccgccagg ccatctccga tgacaccaag ctcgcaggcc
     1561 ggctggccct gtggggtcgg cgcctcgtgg gtgaggcgct gacccaggcc cagcgggtcg
     1621 tcgtggagcg tgaatcggtc gccggcctgc tcgtcgggag cttcggggga gccggtgccg
     1681 acctggccga gctcggccgg atgttcgccc ggctcaccga tgagcacacg cgccggatgg
     1741 cgcgcctcgg gctgtccgcc tgacccgccg gaccggcccc tccccccacg gcgacagcgg
     1801 ggccccgtcc atgccggacg gggccccgct ggggttggtg tgcggtccgg gtcagacctt
     1861 cttggcggtg accgcgccgt agccgacgac gccagcggcg gcgacgacga tgctgatgag
     1921 ccagcggatc cagtcgatgc cgctggtctt ctcgacgccg agcaggctgg ccaggtagta
     1981 gccgacgacc gcgccgacga tgccgatgat g
//
LOCUS       DATAIX010000135         2620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_67454, whole genome shotgun sequence.
ACCESSION   DATAIX010000135 DATAIX010000000
VERSION     DATAIX010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67454"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..775)
                     /locus_tag="VHM65_02075"
     CDS             complement(8..775)
                     /locus_tag="VHM65_02075"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX2704518.1"
                     /translation="MRAPFLVPRLRESLVATGRPWRSLDVHDEIDSTNAECLRLGEPW
                     RVVLAEHQQAGRGRMRRAWEAPAHTSVLLSVLLPGKDAPWGWLPLLAGLAMTRAVWRV
                     AHLELGLKWPNDVLAPADGERKVCGILCEVGNAGVVVGAGLNVDQTRAELPVPTGSSL
                     ALCGARGAAREGLVVAFLDELAAVHTGWGTPQGRPDQEDYRRRCLTIGRVVTVHTPGG
                     AVSGRVSAVDEDGRLLVDGGQGPASYAAGDVEHMSAG"
     gene            800..2407
                     /locus_tag="VHM65_02080"
     CDS             800..2407
                     /locus_tag="VHM65_02080"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX2704519.1"
                     /translation="MATVDVTPSDQSTTSGKLADLKRRVAKVASAGSTRAVNTQHARG
                     KQTARERLQQLLDEGTFIEMDKFARHRSKNFGQEKNRPYGDGVVTGYGQVDGRTVAVF
                     AQDFTVFGGSLGEVFGEKICKVMDFAMKIGCPVVGLNDSGGARIQEGVVSLGLYGDIF
                     YRNVMASGVVPQISLIMGPCAGGAVYSPAITDFTVMVDKTSHMFITGPDVIKTVTGED
                     VGFEDLGGAKAHNTKSGVAHYMGADEVDAIEYVKALLSYLPSNNMDDPPDLGGDHDAD
                     LGVTDEDLWLDTCIPDSAHVPYDMHSVIEHIVDDEEFLEVQPIFAPNIVTGFARVDGM
                     AVGIVANQPMQYAGCLDIDASEKAARFVRTCDAFNVPILTFVDVPGFLPGADQEWDGI
                     IRRGAKLIYAYAEATVPKVTVITRKAYGGAYDVMGSKHLGADINLAWPTAQIAVMGAQ
                     GAVNILYRKELAAAAAKGRDIEGARAAFIKDYEQTLANPYIAAERGYIDRVISPSDTR
                     LNVTRALRALRTKRQSLPPKKHGNIPL"
BASE COUNT          451 a          930 c          871 g          368 t
ORIGIN      
        1 ggcatagcta gccggcagac atgtgctcga cgtctcccgc cgcgtaggac gcaggcccct
       61 gcccgccgtc gacgaggagc cgaccgtcct cgtcgacggc gctgacgcgt ccggacaccg
      121 ctccgcccgg ggtgtgcacg gtgacgacgc ggccgatcgt gaggcacctc cgccgatagt
      181 cctcctggtc gggccggccc tgcggggtgc cccacccggt gtgcaccgcg gcgagctcgt
      241 cgaggaaggc cacgaccagg ccttcgcggg cggcgccccg cgcgccgcac agcgccagag
      301 aggaacccgt cggaacgggc agctccgcgc gagtctggtc cacgttgagc ccggccccga
      361 cgacgacacc ggcgttgccc acctcgcaca ggatcccgca gaccttgcgc tccccgtccg
      421 ccggagcgag cacgtcgttg ggccacttga gcccgagctc gaggtgagcg acccgccata
      481 ccgcacgggt catcgcaaga ccggcgagaa gcggcagcca gccccacgga gcgtccttgc
      541 cgggcagcag caccgacagg agcaccgagg tgtgcgccgg ggcctcccat gcgcggcgca
      601 tccggccacg tccggcctgc tggtgctcgg cgaggacgac ccgccacggc tcgccgaggc
      661 gcagacattc ggcattggtc gagtcgatct cgtcgtggac atccaggctg cgccacggac
      721 gaccggtcgc gaccaacgac tcacgcaggc gtggaacgag gaaaggtgcg cgcatgagga
      781 caaggctagg cttccgggca tggcgacggt tgacgtaacc ccctcggatc agtccacgac
      841 gtcaggcaag ctggccgacc tcaagcgccg tgtcgcgaaa gtggccagcg cggggtcgac
      901 ccgcgcggtc aacacgcagc atgcgcgggg caagcagacg gcccgggagc gcctccagca
      961 gctgctcgac gagggcacct tcatcgagat ggacaagttc gcgcgacacc gcagcaagaa
     1021 cttcgggcag gagaagaacc gcccgtatgg cgacggcgtc gtgacggggt acggccaggt
     1081 cgacgggcgc accgtggccg tgtttgccca ggacttcacg gtcttcggcg gatcgctcgg
     1141 cgaggtgttc ggcgagaaga tctgcaaggt catggacttc gccatgaaga tcggctgccc
     1201 cgtcgtgggg ctcaacgact cgggcggggc ccggatccag gagggcgtcg tctcgctcgg
     1261 gctctacggc gacatcttct accgcaacgt catggcctcc ggtgtcgtcc cgcagatcag
     1321 cctcatcatg ggcccgtgcg cggggggagc ggtctactcc ccggccatca ccgacttcac
     1381 cgtcatggtc gacaagacct cgcacatgtt catcaccggc cccgacgtca tcaagacggt
     1441 gaccggcgag gatgtcgggt tcgaggacct gggcggcgcc aaggcccaca acaccaagtc
     1501 gggcgtggct cactacatgg gcgccgacga ggtcgacgcc atcgagtacg tcaaggcgct
     1561 gctgtcctac ttgccgtcga acaatatgga cgacccgcca gacctcggcg gcgaccacga
     1621 tgccgacctc ggtgtcaccg acgaggacct ctggctcgac acgtgcatcc ccgactcggc
     1681 ccacgtgccc tacgacatgc acagtgtcat cgagcacatc gtcgacgacg aggagttcct
     1741 cgaggtgcag ccgatcttcg cgcccaacat cgtcaccggc ttcgcgcggg tcgacgggat
     1801 ggccgtgggg atcgtcgcga accagccgat gcagtatgcc ggctgcctcg acatcgacgc
     1861 ctccgagaag gccgcccgct tcgtccggac ctgcgacgcc ttcaacgtgc cgatcctgac
     1921 gttcgtcgac gtgccgggct tcctgccggg cgccgaccag gagtgggacg gcatcatccg
     1981 ccggggcgcc aagctcatct atgcctatgc cgaggccacc gtcccgaagg tcaccgtcat
     2041 cacccgcaag gcttatggcg gtgcgtacga cgtcatgggc tccaagcatc tcggcgccga
     2101 catcaacctg gcctggccga cggcacagat cgccgtcatg ggcgcccagg gtgcggtcaa
     2161 catcctctac cgcaaggagc ttgctgcagc ggcagccaaa gggcgcgaca tcgagggggc
     2221 ccgcgcggcc ttcatcaaag actacgagca gacgctggcc aacccctaca tcgcggccga
     2281 gcgtggctac atcgacaggg tcatcagtcc gtccgacacg cggttgaacg tgacccgggc
     2341 cctgcgggcg ctgcgcacca agcgccagag cctgcccccg aagaagcacg ggaacatccc
     2401 gctatgaccg ccaacagcgc tcccgacgcg cctgccgacg ctgtccctgt gatccaggtc
     2461 gtgtccggga cccccagccc cgaggagctg accgctctcg tcgtggtcct gatcgctgcc
     2521 gcgggggacg gcgcgtccgg cataccggac cgttcgtcct cacgatgggc cgcaccaccg
     2581 ggtgcggtgg gttcagcgcg ggaaggacga ggtccgccat
//
LOCUS       DATAIX010000136         3634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_7478, whole genome shotgun sequence.
ACCESSION   DATAIX010000136 DATAIX010000000
VERSION     DATAIX010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7478"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VHM65_02085"
     CDS             complement(<1..910)
                     /locus_tag="VHM65_02085"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX2704520.1"
                     /translation="MREYFLIFVVAAVFTYGATPFVRALALVVGAITPVRDRDVHDTP
                     IPRLGGLAILAGFLAAVLVAMHLPFSVKLFSSGEIKGVVLGAAIVCFVGAIDDVRELD
                     ALTKLSGQVIAAAVMAYQGVQLISLPVFGVTVLPTPVLIAGTVLIVLVTANAVNFIDG
                     LDGLAAGIVGIASLAFFAYSYLISKEYNPPYVFSVATFISAALLGCCVGFLPHNFYPA
                     RLFMGDSGALLLGLLLSSATITMTGSIDPSSVSRNQVTATLIPILVPLAVLSLPLFDM
                     AFAIVRRTRAGQRPWQPDSGHLHHRML"
     gene            complement(942..3008)
                     /locus_tag="VHM65_02090"
     CDS             complement(942..3008)
                     /locus_tag="VHM65_02090"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX2704521.1"
                     /translation="MSEVFDCSTPRGLLAGVAAARDAMNRGRLVVFPTDTVYGVGCDA
                     FNHDAVRALLDAKGRGPAIPPPVLIPSSRTVDGLARSVPDWARRLMDEFWPGALTVVL
                     PVQPSLAWDLGETNGTVALRVPDHEIALGLLEQAGPSAVTSANLTGRPAATTVAEAQQ
                     MLGESVAVYLDGGPAAGGAASTILDCTGEHPVVLREGALSWADLVARVALPDAAIATA
                     TVDQPIEPATTSLDTSRSMTPMSSDTMSSDGLSSDGLSSDTRPSDTATPFYGSDFAAL
                     QAFDPDIAAVLLSEVGRIRSGLQLIASENLSSPEVLTALGSTLSNKYAEGYPGRRYYG
                     GCAEVDKAEQIAIDRCTALFGADHANVQPHSGANANLAVYGAFLKPGETLLAMSLPHG
                     GHLTHGSKVSFSGKWFNAVHYGVDRETEDIDYDQVRDLALEHRPKVILAGGSAIPRLI
                     DFAFFRALADEIDAIFWVDAAHFIGLVAGQAIPSPVPFADVVSFTTHKVLRGPRSGAI
                     VCTAEHAAAIDKAVFPMMQGGPQMHSIAAKAVNFKECATPEYAAYARQVLANASTLAL
                     SLGEHGIRPTTGGTDTHLSLHDLQGVGVTGRDAEARCDGAGIVLNKNAIPFDPQPPSI
                     ASGIRVGSPCVTTQGMGTEQMVDIARLIHRAVTAGDADPAHAVSAEVRAEVTDLVTRF
                     PAYPRA"
     gene            complement(3018..>3634)
                     /gene="prmC"
                     /locus_tag="VHM65_02095"
     CDS             complement(3018..>3634)
                     /gene="prmC"
                     /locus_tag="VHM65_02095"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX2704522.1"
                     /translation="FRRLTLSVGPGVFVPRPETELLVGHALRALSGVDRPLVVDLCTG
                     SGAIALSVKSECPRARVVAVELSAHAHAWATENVTRTSLAVELHLGDATTALPELDGT
                     ADVVVSNPPYIPVGAVPVDPEVRDHDPEVALYGGSSDGLAIPLQVAERAASLLRSGGT
                     LVMEHGDTQGAALVRALAETGWWTSVTDHPDLVGKPRHVVARRA"
BASE COUNT          556 a         1168 c         1422 g          488 t
ORIGIN      
        1 ccagcatccg gtggtgcagg tgtcccgagt ccggctgcca cggccgttga ccggcgcggg
       61 tgcgccgcac gatggcaaag gccatgtcga acagcggcag cgagagcacg gcgagcggaa
      121 cgaggatcgg gatcagggtc gcggtgacct ggttgcggct caccgagctg gggtcgatgc
      181 tgcccgtcat cgtgatcgtc gccgaggaca gcagcaggcc gaggagcagg gcgccggagt
      241 cgcccatgaa gagccgggcc gggtagaagt tgtgcggcag gaagccgacg cagcacccga
      301 gcagcgccgc gctgatgaag gtcgccaccg agaagacgta aggcgggttg tactccttgg
      361 agatgagata ggagtaggcg aagaacgcca gcgaggcgat gcccacgatc cccgccgcga
      421 gcccgtcgag cccgtcgatg aagttcaccg cgttggccgt cacgagcacg atgagaacgg
      481 tcccggcgat gagcacgggc gtcgggagca cggtgacccc gaagaccggc agcgagatga
      541 gctgcacccc ctggtaggcc atgactgccg ccgcgatgac ctgcccggac agcttggtca
      601 gggcgtcgag ctcacggacg tcgtcgatgg cgccgacgaa gcagacgatg gcagcgccga
      661 gcacgacgcc cttgatctca cccgagctga acagcttgac cgagaagggg aggtgcatgg
      721 cgacgaggac ggcggccagg aagccggcga ggatcgccag ccccccgagc cgcgggatcg
      781 gcgtgtcgtg gacgtcgcgg tcacggacgg gggtgatggc ccccacgacg agcgcgagtg
      841 cccgcacgaa gggcgtcgcg ccatatgtga acacggcggc gacgacaaag atgaggaagt
      901 actcgcgcac ctagcgcgct cccgcctcac cagggcacgt cctaggcacg cgggtatgcc
      961 gggaagcggg tcacgaggtc ggtcacctcg gcccgcacct cggcggagac ggcgtgagcc
     1021 gggtcggcgt ccccggccgt cacggcgcgg tggatgagcc gggcgatgtc gaccatctgc
     1081 tcggtcccca tgccctgcgt ggtgacgcac ggcgacccga cgcggatccc cgaggcgatg
     1141 ctcggcggct gcgggtcgaa ggggatggcg ttcttgttga gcacgatgcc ggccccatcg
     1201 cagcgggcct cggcgtcccg gccggtcacc ccgacgccct gcaggtcgtg caggctcagg
     1261 tgggtgtccg tgccgcccgt ggtcggccgg atgccgtgct cgcccaggga gagggcgagg
     1321 gtgctggcgt tggcgaggac ctggcgggcg tacgcggcat actccggggt cgcgcactcc
     1381 ttgaagttga ccgccttggc ggcgatcgag tgcatctgcg gaccgccctg catcatcggg
     1441 aagaccgcct tgtcgatcgc ggcggcgtgc tcggccgtgc agacgatcgc accggagcgg
     1501 gggccgcgca ggaccttgtg ggtcgtgaag ctgacgacgt cggcgaaggg caccgggctc
     1561 gggatcgcct gtccggcgac gagaccgatg aagtgggcgg catcgaccca gaagatcgcg
     1621 tcgatctcgt cggcgagggc gcggaagaac gcgaagtcga tgagccgtgg gatggccgac
     1681 ccgccggcga ggatcacctt gggccggtgc tccagggcga ggtcgcggac ctggtcgtag
     1741 tcgatgtcct cggtctcgcg gtcgacgccg tagtggacgg cgttgaacca cttgccggag
     1801 aaggacacct tggagccgtg ggtgagatgc ccaccgtggg gaagcgacat cgcgaggagg
     1861 gtctcgcccg gcttgaggaa cgcgccgtac acggccaggt tggcgttggc ccccgagtgc
     1921 ggctggacgt tggcgtggtc ggcgccgaag agcgccgtgc agcggtcgat ggcgatctgc
     1981 tcggccttgt cgacctcggc gcagccgccg tagtagcgcc gcccggggta gccctctgcg
     2041 tacttgttcg acagggtcga gccgagggcg gtgagcacct cgggcgagga gaggttctca
     2101 ctcgcgatga gctgcaggcc gctgcggatc cggccgacct cggagaggag cacggcggcg
     2161 atgtcggggt cgaaggcctg cagggccgcg aagtccgagc cgtagaaggg cgtggcggtg
     2221 tcgctgggcc gggtgtcgct ggacaggccg tcgctggaca ggccgtcgct ggacatggtg
     2281 tcgctggaca tgggcgtcat actccgtgag gtgtcgaggg aggtcgtcgc tggctcgatc
     2341 ggttggtcca ctgtagccgt ggcgatcgcg gcgtccggca gcgcgacgcg cgcgacgaga
     2401 tcggcccagg acagggctcc ctcacgcagg accacggggt gctcccccgt gcagtcgagg
     2461 atggtcgagg ccgcccctcc cgccgcgggc cctccgtcga ggtagaccgc gaccgactcc
     2521 cccagcatct gctgggcctc ggcgacggtc gttgcggcgg gccggccggt gaggttggcg
     2581 ctcgtcacgg cgctggggcc ggcctgctcg agcaggccga gggcgatctc gtggtcgggg
     2641 acgcgcagcg cgaccgtgcc gttggtctcg ccgaggtccc acgcgagcga gggctggacg
     2701 ggcaggacga cggtgagcgc ccccggccag aactcgtcca tgagccggcg ggcccagtcc
     2761 gggacggagc gcgcgagacc gtcgaccgtg cgcgacgagg ggatcaggac cggtgggggt
     2821 atcgcggggc cgcggccctt ggcgtcgagc agggcccgga cggcgtcgtg gttgaacgcg
     2881 tcgcacccca cgccatacac cgtgtcggtc gggaagacga cgagccgccc ccggttcatc
     2941 gcgtcgcggg ctgccgcgac tccggccagc agcccccgcg gtgtggagca gtcgaacacc
     3001 tcactcatgg ccaaacccta tgcgcgccga gccacgacgt gccggggctt gccgacgagg
     3061 tcggggtggt cggtgacgct cgtccaccag ccggtctcgg ccagcgcgcg cacgagggcc
     3121 gcgccctggg tgtcgccatg ttccatgacg agggtcccgc cgctgcgcag gagggaggcg
     3181 gcgcgctcgg ccacctgcag cgggatggcc agcccgtcgc tgctcccgcc gtacagcgcg
     3241 acctcggggt cgtggtcgcg cacctcggga tcgacgggca cggctcccac cgggatgtat
     3301 ggcgggttgc tcaccacgac atcggccgtg ccgtcgagct cgggcagagc cgtcgtggcg
     3361 tcgccgaggt gcagctcgac ggcgagcgag gtgcgggtga cgttctcggt agcccaggcg
     3421 tgggcgtgcg cggagagctc caccgcgacg acccgcgcac ggggacactc ggacttgacc
     3481 gagagggcga tcgcccctga gccggtgcac aggtcgacga cgagggggcg gtccacgccg
     3541 ctgagggcgc gaagggcatg tccgacgagg agctcggtct cgggcctcgg gacgaagacg
     3601 ccgggcccga ccgacagggt cagccggcgg aagt
//
LOCUS       DATAIX010000137         1638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_271565, whole genome shotgun sequence.
ACCESSION   DATAIX010000137 DATAIX010000000
VERSION     DATAIX010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271565"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..977
                     /locus_tag="VHM65_02100"
     CDS             159..977
                     /locus_tag="VHM65_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX2704523.1"
                     /translation="MSDLLITSPANGRLRALVGLRRRRTREESGTTLVEGFEELTLAL
                     DAGVEPRVLFYCPELHGADERHSELIARARAAGAETIQVGRVAFERVAYREGPDGFLA
                     VVPSTSTSLAALRPTRDGLSLVCQGVEKPGNLGAMLRSADAAGVDAVLAADPVTDWGN
                     PNVVRASKGTVFSVPCVSATTEQTLQWLAEHEVTLVATTPDTDVFYTDVDYSGRVAVA
                     VGAEKHGLTEDLLAAAGHRVRIPMFGRANSLNVAASAAIVLFEAVRQRRSRSTY"
     gene            1062..>1638
                     /locus_tag="VHM65_02105"
     CDS             1062..>1638
                     /locus_tag="VHM65_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2704524.1"
                     /translation="MRVDHVSYAAEHDGLRATADRLAKLIGVDSVDGGVHPRFGTRNI
                     ILPLADERYVEVVEVLDHPASDKAPFGQAVRARSEAGGGWLGWVIRIDDITVAEHRVG
                     RSSVEGYRHRPDGAELRWRQVGVKGLMADPQLPYFVQWMDPSLHPSRSGRSDCHLVGL
                     QIAGDPGRVKHWLGLPDGCEPSTLQFDFVAPH"
BASE COUNT          227 a          577 c          572 g          262 t
ORIGIN      
        1 gtcaaagagg tcgccgacga ggtcaccgca aatgtcgccg aggacggact ggtgccggtc
       61 ctgcgctcgc tgctcgaggc atgacccagg tcccgcgctg acgaagactt cggctggaca
      121 gggcccctcc gtcgtcatcg ggtgccatga tgagcccgat gtcggacctc cttatcacct
      181 caccggccaa cgggcggctc agggcgctcg tcgggctgcg acggcgacgc acccgcgagg
      241 agtcggggac gacactcgtc gaggggttcg aggagctcac cctcgcgctc gacgcggggg
      301 tggagccccg ggtcctcttc tactgccccg agctgcatgg cgcggacgag cgccactctg
      361 agctgattgc gcgtgctcgc gctgccgggg ccgagacgat ccaggtgggg cgggtcgcct
      421 tcgagcgggt cgcctatcgg gagggccctg acggcttcct cgccgtcgtc ccctcgacga
      481 gcacgtcgct cgctgccctc aggccgaccc gcgacgggct gagcctggtc tgtcagggcg
      541 tcgagaagcc gggcaacctc ggcgcgatgc tgcgcagcgc cgatgccgcg ggagtcgacg
      601 ccgtgctcgc cgccgacccg gtgaccgact ggggcaaccc caatgtcgtg cgcgcgagca
      661 agggcacggt cttctccgtt ccctgcgtca gtgccacgac ggagcagact ctgcagtggc
      721 tggccgagca cgaggtcacg ctggtggcga cgacgcccga cacggacgtg ttctacaccg
      781 acgtcgacta ctccggccgg gtcgccgtcg ccgtcggggc cgagaagcat ggcctgaccg
      841 aggacctgct tgccgccgcc ggccatcggg tgcggatccc gatgttcggt cgggccaact
      901 cgctcaacgt cgcggcgtcg gcggccatcg tgctcttcga ggcggtccgc cagcgccggt
      961 cgcgctcgac gtattgacga cgaagcgtcc cagcagtctc aggcgtccca gcttcgggac
     1021 ctctgtcaag agcacacctt tcggtgtgcc acgatgggcc catgcgggtt gaccatgtct
     1081 cgtatgcagc cgagcacgac ggccttcggg ctacggccga ccgccttgcc aagctgatcg
     1141 gggtggactc cgtcgacggc ggagtgcacc cccggttcgg gacgcgcaac atcattctgc
     1201 cgctcgcgga cgagcgttat gtcgaggtcg tcgaggtgct cgaccatccg gcctccgaca
     1261 aggccccctt cggtcaggcc gtgcgcgccc ggtccgaggc cggcggtggc tggctcgggt
     1321 gggtgatccg gatcgacgac atcaccgtgg ccgagcaccg ggtcggccgc tcgtcggtcg
     1381 agggctaccg ccaccggccc gacggcgccg agctgcgctg gcgccaggtg ggtgtcaagg
     1441 gcctcatggc cgatccgcag ctgccctact tcgtccagtg gatggacccg agcctgcacc
     1501 cctcgcggtc cggccggtcg gactgccacc tcgtcggtct gcagatcgcc ggtgacccag
     1561 gccgggtcaa gcattggctc ggcctgccgg acggctgcga accttcgacg ttgcagttcg
     1621 acttcgtcgc accacacg
//
LOCUS       DATAIX010000138         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_7579, whole genome shotgun sequence.
ACCESSION   DATAIX010000138 DATAIX010000000
VERSION     DATAIX010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7579"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1501
                     /locus_tag="VHM65_02110"
     CDS             <1..>1501
                     /locus_tag="VHM65_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704525.1"
                     /translation="VHIGRVWVGPGATVGGRSTLGPGTRIGAGAEISPGSGVSGKVPA
                     GELWTGSPAVRQGRAGTEFPTQRPARAVHWVAGYGVSSVLLAGVPLVSALPALWLVLH
                     AAAGRRSLGSAPVAALAWTPVATLAGLLTLALLTILAVRLLGLGVRPGYHPVRSRVGF
                     QVWATERLLDSARTLLFPLYASLLTPGWLRALGARVGHDVEASTVLLLPVMTTIGDGA
                     FLADDTMIASYELGGGWLHIEPAKIGKRAFLGNSGMAAPGRTVPKNGLVAVLSTAPAK
                     SKAGTSWLGSPPVRLRRTASETDQSRTYHPPTRLKVARALVEACRLVPVTATVALAVT
                     VAATYEWLWFRVGLLATAALGGFVLLCAGLVAGALAVVAKWVLVGRLRVGETPLWSSF
                     VWRNEVVDAFVEIVAAPDERAPQRGLADSKAPDEDPLGRDGKRPRDQSGEEQDEPAEC
                     RRGKQTVAEPEPFVGRRDGHRQGHRGGDRNEPARLDEGTGDLEPGRWVVG"
BASE COUNT          172 a          586 c          531 g          212 t
ORIGIN      
        1 ggtccacatc ggccgggtct gggtcggacc tggcgccacc gtcggcgggc gcagcacgct
       61 gggccccggc acccggatcg gcgcgggcgc cgagatcagc cccgggtccg gcgtctcggg
      121 caaggtgccc gccggtgagc tctggaccgg ttcgcccgcg gtccgccagg gcagggccgg
      181 caccgagttc cccacgcagc gaccggcccg ggccgtgcac tgggtggccg ggtatggcgt
      241 ctcctccgtc ctgctcgccg gcgtcccgct cgtctcggcc ctgcctgccc tctggctcgt
      301 cctgcatgcc gccgcgggcc gcagatcgct cgggagcgca cccgtcgcgg ccctcgcctg
      361 gaccccggtc gcgacgctgg ccgggctgct caccctcgcc ctgctgacga tcctcgcggt
      421 ccggctcctc ggcctcggcg tccggcccgg ctaccacccc gtgcgcagcc gggtcggctt
      481 ccaggtgtgg gccaccgagc ggctcctcga cagcgcccgg accctgctct tcccgctcta
      541 tgccagcctg ctcaccccgg ggtggctccg cgctctcggc gccagggtcg gccacgacgt
      601 cgaggcctcg accgtcctgc tcctgccggt catgacgacg atcggcgacg gggccttcct
      661 cgccgacgac acgatgatcg cgtcctacga gctcgggggt ggctggctgc acatcgagcc
      721 ggccaagatc ggcaagcggg ccttcctcgg caactcgggc atggccgcgc ccggccgcac
      781 cgtgcccaag aacgggctcg tggccgtcct gtccaccgct ccggcgaagt ccaaggcggg
      841 cacctcgtgg ctcggctccc cgccggtccg gctcaggcgc accgcgagcg agaccgacca
      901 gagccggacc taccacccac cgacccggct caaggtcgcc cgtgccctcg tcgaggcgtg
      961 ccgcctcgtg ccggtcaccg ccaccgtggc cctcgcggtg accgtcgcgg cgacctacga
     1021 atggctctgg ttccgcgtcg gcctgctcgc cacggccgcc ctgggcgggt tcgtcctgct
     1081 ctgcgccgga ctggtcgccg gggcgcttgc cgtcgtggcc aagtgggtcc tcgtcgggcg
     1141 cctgcgggtc ggggagaccc cgttgtggag ctcgttcgtc tggcgcaacg aggtcgtcga
     1201 cgccttcgtc gagatcgtcg ccgcgccaga cgaacgtgct ccacagcggg gtctcgccga
     1261 ctcgaaggcg cccgacgagg acccacttgg ccgcgacggc aagcgccccc gcgaccagtc
     1321 cggcgaagag caggacgaac ccgccgagtg ccgccgtggc aagcagaccg tagcggagcc
     1381 agagccattc gtaggtcgcc gcgacggtca tcgccagggc caccgtggcg gtgaccggaa
     1441 cgagccggca cgcctcgacg agggcacggg cgaccttgag ccgggtcggt gggtggtagg
     1501 t
//
LOCUS       DATAIX010000139         3801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_211923, whole genome shotgun sequence.
ACCESSION   DATAIX010000139 DATAIX010000000
VERSION     DATAIX010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211923"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VHM65_02115"
     CDS             complement(<1..450)
                     /locus_tag="VHM65_02115"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX2704526.1"
                     /translation="MQLLLVRHGQTRSNVEMLLDTAHPGAPLDETGHAQAAALAGRLA
                     VDPVDGIYASDLTRTRQTAAPLAAARGITPVVLPGLREIQAGDDELSVDWTRYVAALG
                     AWRDDPSVRIPGGENGIEFMTRFDEAVGQVASAGHASAVLFTHGAALR"
     gene            complement(598..1038)
                     /locus_tag="VHM65_02120"
     CDS             complement(598..1038)
                     /locus_tag="VHM65_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX2704527.1"
                     /translation="MPTRFDPFREMDRFFTTAMRPPAATAMPMDLYRDGEQFIVKIDL
                     PGVDPSTVDVDVEDRTLTVRAERREDLPEQVQWLSHERPTGTFARQLTLGYGVALDRI
                     EAEYADGVLTLRVPVAEEAKPRRISVTHASGRTPIAGETVAPVG"
     gene            complement(1154..1543)
                     /locus_tag="VHM65_02125"
     CDS             complement(1154..1543)
                     /locus_tag="VHM65_02125"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HEX2704528.1"
                     /translation="MADLTILHNARCSTSRAALETIVAAGISAEVLTYLTHPLDAAAL
                     GDLVDKLEDPPTDLVRRDPYFAGLGLTDADVATREQVVAVLAEHPRLMQRPVLVRGSR
                     AIIGRPKDRVAAFLTEHPRNTPAPPQR"
     gene            1673..>3801
                     /locus_tag="VHM65_02130"
     CDS             1673..>3801
                     /locus_tag="VHM65_02130"
                     /inference="COORDINATES: protein motif:HMM:NF023454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HEX2704529.1"
                     /translation="MSLTDALAPAEQRSPEQFDPDATYAALVEWAADQGITLYPAQQE
                     ALLELATGADVILSTPTGSGKSLVAVGAHLIAMAQGQRTYYTAPIKALVSEKFFALCD
                     VFGAENVAMLTGDAAVNVGAPLICCTAEVLANIALREGRGADIGQVVMDEFHFYAEPD
                     RGWAWQVPLLTLPRTQFLLMSATLGDASFFRDDLTRRTGRPTAVVASAQRPVPLSFQW
                     ALTPLHETLDELLTTHQAPIYVVHFTQAAALERAQALMSTNVCTRAEKDAIAIQIKGF
                     RFARGFGATLSRLVRHGIGVHHAGLLPKYRRLVEQLAQQGLLKVICGTDTLGVGINVP
                     IRTVVFTGLSKYDGTRQRLLKAREFHQIAGRAGRAGFDTAGTVVVQAPEHVIENHRLL
                     TRAGDDAKKLRRVQRKPAPEGFVSWTEDQFERIVAAEPEPLQSRMQITYSMILNIISA
                     PGDPFVAVRDLLRDNHEDRRRQARLARRAVEIARSLLAAGVVQRLPEPDGTGRTLALT
                     MDLQRGFALNQPLSAFALAAIELLDPASPSYALDVVSVIEATLEDPRGVLGAQQWLAR
                     GQAVAEMKADGLEYDERMELLDDVSWPKPLEELLTAALELYRHTHPWISERDLSPKSV
                     ARDLHERAMTFGEYVAFYKLTRAEGMVLRYLSDAYKALRQTVPVGARTEELTDIVEWL
                     GEVVRQTDSSLLEEWEQLTSGYDGQLTS"
BASE COUNT          571 a         1370 c         1320 g          540 t
ORIGIN      
        1 ccgcagagca gccccgtggg tgaagagcac tgcgctcgca tggcccgccg aggccacctg
       61 cccgaccgcc tcgtcgaacc gggtcatgaa ctcgatcccg ttctcaccgc cggggattcg
      121 cacgctgggg tcgtcgcgcc aggcgcccag cgcggccacg taccgggtcc agtcgacgct
      181 cagctcgtcg tcaccggcct ggatctcgcg caagccgggc aggacgacag gtgtgatccc
      241 ccgcgccgcg gccagaggcg cggcggtctg ccgggtgcgg gtgagatccg aggcgtagat
      301 cccatcgacg gggtcgacgg ccagccgacc tgccagcgca gcagcctgag cgtgtccagt
      361 ctcgtcgagc ggcgcccccg ggtgggccgt gtcgaggagc atctcgacgt tggaccgcgt
      421 ctggccgtgc cggacgagca ggagctgcat ggccatcacc ctagggcgga ggggagcggc
      481 ctcctggtca ggggtaccac tgggccggcg actcgaccaa gggggcgcct gcgccacgcc
      541 cgagatgcgg cgcgaggtca gaccccggcc acggctgagc cgtggctcag ccgtggctca
      601 cccgaccggg gcgacggtct cgccggcgat cggggtgcgc ccactcgcgt gggtcacgct
      661 gatccgccgg ggcttggcct cctcggcgac cgggacccgc agggtgagca cgccgtcggc
      721 gtactcggcc tcgatgcggt cgagggcgac gccatacccc agggtgagct ggcgggcgaa
      781 ggtgccggtc gggcgctcgt gcgagagcca ctggacttgc tcgggcaggt cctcacggcg
      841 ttcggcgcgg acagtgaggg tgcgatcctc gacgtcgaca tcgacggtcg aaggatcgac
      901 cccgggtagg tcgatcttga cgatgaactg ctcaccgtct cggtagaggt ccatcggcat
      961 cgcggtcgct gcgggcggcc gcatggcggt ggtgaagaag cggtccatct cacggaacgg
     1021 gtcgaagcgg gtaggcatgg ccagcatcct cctgacatga cagggccaac agggtccggg
     1081 accggagcgc gagcgccccg ggcccgcagc tacctcgtgc cacgcctgcg ttagcactca
     1141 gcatccatga gtgctaacgc tgcgggggcg ctggagtgtt cctgggatgc tcggtgagga
     1201 aggcggcgac ccggtccttc ggccgcccga tgatcgcccg gctgccgcgg accaggacgg
     1261 gccgctgcat gagccgcggg tgctcggcca gcacggcgac gacctgctcg cgagtggcca
     1321 cgtcggcgtc ggtgaggccg agcccggcga agtacgggtc gcggcggacc aggtcggtcg
     1381 gcgggtcctc gagcttgtcg accaggtcgc cgagtgcggc ggcatcgaga gggtgggtga
     1441 gataggtgag cacctcggcc gagatgcctg ccgcgacgat ggtttccagg gccgcgcggg
     1501 aggtcgagca gcgggcgttg tgcaggatcg tgaggtcggc catggcgtga gactaccgag
     1561 gtcgatctgg ccgggccgga ccggccagat caacttcggt gccggggaac ccgccacgga
     1621 gcacgggttc cccggcgcag gcagggcccg gcagtagtgg acgataggga cgatgtccct
     1681 caccgacgcc ctggcccccg cggagcagcg cagccctgag cagttcgacc ccgacgcgac
     1741 atacgcggcc ctggtcgagt gggccgcaga ccagggcatc acgctctacc cggctcagca
     1801 ggaagcgctc ctcgagctcg ccacgggggc cgacgtcatc ctctcgacgc cgaccgggtc
     1861 ggggaagtcg ctcgtggccg tcggcgccca tctcatcgcg atggcccagg gccagcgcac
     1921 ctactacacc gcgcccatca aggccctcgt gagcgagaag ttcttcgccc tgtgcgacgt
     1981 cttcggggcc gagaacgtcg cgatgttgac cggtgacgcc gccgtcaacg tgggcgcgcc
     2041 cctcatctgc tgcacggctg aggtcctggc caacatcgcg ctgcgcgagg gcaggggcgc
     2101 cgacatcggc caggtggtca tggacgagtt ccacttctac gccgagcccg accgcgggtg
     2161 ggcctggcag gtgccgttgc tcaccctgcc caggacccag ttcctgctca tgtcggccac
     2221 cctcggtgac gcgtcgttct tccgcgacga cctcacccgg cgcaccggcc gccccaccgc
     2281 tgtcgtggcc agtgcccagc gccccgtccc cctgtccttc cagtgggcgc tcacgcccct
     2341 gcacgagacc ctcgacgagc tgctcacgac ccaccaggcc ccgatctacg tcgtgcactt
     2401 cacccaggcc gcggccctcg aacgcgccca ggcactcatg agcaccaacg tctgcacccg
     2461 cgccgagaag gacgcgatcg ccatacagat caaggggttt cgctttgccc gaggtttcgg
     2521 cgccaccctg agccggctgg tgaggcacgg catcggagtg caccacgcgg gcctgctccc
     2581 gaagtaccgg cgcctcgtcg agcagctcgc ccagcagggc ctgctcaagg tcatctgcgg
     2641 tacggacacg ctcggggtcg gcatcaacgt gcccatccgg accgtcgtgt tcaccggcct
     2701 gagcaagtat gacgggacgc gtcagcgcct gctcaaggcc cgcgagttcc accagatcgc
     2761 cggccgcgcc ggacgggccg gcttcgacac ggccggcacc gtcgtcgtcc aggcacccga
     2821 gcacgtcatc gagaaccacc ggttgctcac cagggccggg gacgacgcca agaagcttcg
     2881 ccgggtccag cgcaagcccg cccccgaggg cttcgtgtcc tggaccgagg accagttcga
     2941 gcggatcgtc gccgcggagc cggagccgct gcagtcccgc atgcagatca cctactcgat
     3001 gatcctcaac atcatctcgg cccccggcga ccccttcgtc gccgtgcggg acctgctccg
     3061 ggacaaccac gaggaccgcc ggcgccaggc ccgcctcgcc cgccgcgccg tcgagatcgc
     3121 ccgctcgctg ctcgctgcgg gagtcgtgca gcgcctcccc gagccggacg ggaccggccg
     3181 cacgctcgcc ctgacgatgg acctgcagcg cgggttcgcc ctcaaccagc cgctgtccgc
     3241 cttcgccctc gccgcgatcg agctgctcga ccccgcgtcc ccgtcatacg cgctcgatgt
     3301 cgtcagcgtc atcgaggcga ccctggagga cccgcggggc gtgctcggcg cccagcagtg
     3361 gctcgcccgc ggccaggccg tcgccgagat gaaggccgac gggctcgagt acgacgagcg
     3421 gatggagctc ctcgacgacg tctcctggcc caagccgctc gaggagctgc tcaccgcggc
     3481 actcgagctc taccgtcata cgcacccgtg gatctccgag cgcgacctgt cgccgaagtc
     3541 ggtcgcgcgt gacctgcacg aacgggcgat gaccttcggt gagtacgtcg cgttctacaa
     3601 gctgacccgc gccgagggca tggtgctgcg ctatctgtcc gacgcctaca aggcgctgcg
     3661 ccagaccgtc cccgtcggcg cccgcacaga ggagctcacc gacatcgtcg agtggctcgg
     3721 ggaggtcgtc cgccagaccg actccagcct gctcgaggag tgggaacagc tcaccagtgg
     3781 gtatgacggt cagctcacct c
//
LOCUS       DATAIX010000140         1560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_67719, whole genome shotgun sequence.
ACCESSION   DATAIX010000140 DATAIX010000000
VERSION     DATAIX010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67719"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="VHM65_02135"
     CDS             <1..1381
                     /locus_tag="VHM65_02135"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF015904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX2704530.1"
                     /translation="HGAVLTDLGVNPAMGLGDLYAKISDLPDEHRMQIETDIQAVYDA
                     RPAIAMVNSDKGITNLHVPSDIIVDASMPVVVRDSGQMWNADGHLQATLAMVPDRCYG
                     PMYAEVFQNCRTHGALDPATMGSVPNVGLMAQKAEEYGSHPTTFIMTAAGTVRVVDAA
                     GAVLMSHGVEQGDVWRMSRVRDIPIQDWVKLAVTRARLTGAPACFYLDSKRAHDANVI
                     AKVQRYLQDHDTSGLEIRVLAPVEAIRYCLERIREGKDTISVTGNVLRDYLTDLFPIL
                     ELGTSAKMLSIVPLLAGGGLFETGAGGSAPKHVQQFVQENYLRWDSLGEFSALGASLE
                     HLAVSCANPKAQVLADTLDVAIATFLDENKSPARKVGQIDNRGSHFYLALYWAEALAA
                     QTADAQLRERFAPAAKALRENEARINDELIGVQGHPVDMGGYYRPDVAKTSAAMRPSP
                     TLNTIIDAL"
BASE COUNT          264 a          559 c          500 g          237 t
ORIGIN      
        1 gcacggtgcc gttctcaccg acctcggcgt gaaccccgcc atggggctcg gcgacctcta
       61 cgccaagatc tccgacctgc cggacgagca tcgcatgcag atcgagaccg acatccaggc
      121 cgtgtatgac gctcgcccgg cgatcgccat ggtgaactcg gacaagggca tcaccaacct
      181 gcacgtcccc agcgacatca tcgtcgacgc gtccatgccc gtcgtcgtgc gcgactccgg
      241 gcagatgtgg aacgccgacg ggcatctgca ggccaccctc gcgatggtgc cggatcgctg
      301 ctacggcccg atgtacgccg aggtcttcca gaactgccgg acccacgggg cgctcgaccc
      361 ggcgaccatg gggtcggtcc ccaacgtcgg cctcatggcg cagaaggccg aggagtacgg
      421 ctcgcacccg acgaccttca tcatgaccgc cgccggcacg gtccgggtcg tcgacgcggc
      481 gggcgcggtc ctcatgtcgc acggcgtcga acagggcgac gtctggcgga tgtcccgggt
      541 gcgcgacata ccgatccagg actgggtcaa gctcgccgtg acccgggcaa ggctgaccgg
      601 cgcgccggcc tgcttctacc tggacagcaa gcgcgcccac gacgccaacg tcatcgccaa
      661 ggtgcagcgc tacctccagg accacgacac gagcggtctg gagatccggg tcctggcgcc
      721 cgtcgaggcg atccgctact gcctggagcg gatccgcgag ggcaaggaca cgatctcggt
      781 gaccggcaac gtcctgcgtg actacctcac cgacctcttc ccgatcctcg agctcggcac
      841 gagcgcaaag atgctctcga tcgtcccgct ccttgcgggc ggcggcctgt tcgagaccgg
      901 cgccggcggg tcggcgccca agcacgtcca gcagttcgtc caggagaact acctgcggtg
      961 ggactccctc ggtgagttct cggcgctcgg ggcctcgctg gagcacctcg cggtgagctg
     1021 tgccaacccc aaggcgcagg tcctcgccga caccctcgac gtcgccatcg ccacgttcct
     1081 cgacgagaac aagtccccgg cgcgcaaggt cggccagatc gacaaccgcg gcagccactt
     1141 ctacctggcg ctctactggg ccgaggccct ggcggcgcag accgccgatg cgcagctgcg
     1201 ggagcggttc gcgccggctg ccaaggcgct gcgcgagaac gaggcgagga tcaacgacga
     1261 gctgatcggc gtccagggcc atcccgtgga catgggcggc tactaccgtc cggacgtcgc
     1321 gaagacctcg gccgccatgc gcccgagccc caccctcaac accatcatcg acgcgctgtg
     1381 aggtgcagtc ccacgtaacc tgacgggctc cccgcctaca gattctcgcc gagttcccga
     1441 gttcccgagt tcccgggtgg ccgcgggtcg cgtcagatgt gactttgccc agcgtgatct
     1501 gtagttccag atggcccatt gcagcgcaaa ctacagattt ggctgagttt gggcagtagg
//
LOCUS       DATAIX010000141         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_7671, whole genome shotgun sequence.
ACCESSION   DATAIX010000141 DATAIX010000000
VERSION     DATAIX010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7671"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1584)
                     /locus_tag="VHM65_02140"
     CDS             complement(<1..>1584)
                     /locus_tag="VHM65_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX2704531.1"
                     /translation="IGSLAAGALAIPLNLLVTHANAASATVVAIVGAGVQLYSIWYLA
                     DDKRYGAFAATVSMFTAAMLLVVMSGDLVLTLIGWEVMGWGSYLLIGHLTAKESARRA
                     AVKAFLVTRFADIGFVLGVVMLAVAAGTTQLSKVLVTTGAAVTTALVLLLMGVAGKSG
                     LVPFHDWLPDAMEGPTPASALIHGATMVAAGTVVVSSLFPLYAEHPPARAVLAVLTSV
                     TMVWAALLAFGQSDLKRMLAYSTLSQVAIMLSALSVAPLEVGPGAGLGHLYGHAFFKA
                     LLFLGAGWLAAVVGAGTAFAGLRGRLAPRSALGLSMAVGLASLAGVPPLVGFFSKETV
                     VGTAEESALNGGGLAAWLVLVALLVTIPLTAAYATRAWLVLAGARRPAGVAEPVAAAQ
                     PVTAGHDEHGPVGRVASYTVIALALFTVLGGLLLVLARTGVHLAPVTTVLALVLVAVA
                     GVLVYRRYAASGEDPALALGARTLERADAGYGMDSVYVRVATWTQAAGRLVVALDRDV
                     VDAYPRAASRATVLLGRGLD"
BASE COUNT          270 a          536 c          614 g          164 t
ORIGIN      
        1 gtccaggccc cggccgagca ggaccgtcgc gcgggacgcg gcccgggggt aggcgtcgac
       61 gacgtcacgg tcgagagcga cgacgagccg gccggccgcc tgcgtccagg tcgcgacccg
      121 gacgtagacc gagtccatgc catacccggc atccgcgcgc tccagtgtgc gcgcgccgag
      181 ggccagggcc gggtcctcgc cggacgcggc gtacctgcgg tagacgagga ccccggctac
      241 cgccacgaga acgagtgcga ggacggtcgt caccggtgcg aggtgcacgc cggttcgggc
      301 cagaaccagg agcaggccgc cgagcacggt gaagagggca agagcaatga ccgtgtatga
      361 cgccacgcgc cccaccggcc cgtgctcgtc atggccggct gtgacgggct gggcggccgc
      421 gacgggctcg gcgacgccgg ccggcctgcg cgccccggcg agcacgagcc aggctcgggt
      481 cgcataggcg gccgtgagcg ggatggtcac gagcagggcg accaggacga gccacgccgc
      541 gagcccgccg ccattgagcg cactctcctc ggccgtcccg acgacggtct ccttgctgaa
      601 gaagccgacg agcggcggga ccccggccag ggaggccagg ccgaccgcca tcgacaggcc
      661 gagcgcgctg cgcggcgcga gccggccccg caggccggcg aacgcggtcc ctgcgccgac
      721 gaccgcggcg agccatccgg ctccgaggaa gagcagcgcc ttgaagaacg cgtggccgta
      781 gaggtgcccg agcccggctc cgggaccgac ctccagcggc gcgaccgaca gggcgctgag
      841 catgatcgcg acctggctca gcgtcgagta ggcgagcatc cgcttgaggt cggactgtcc
      901 gaaggcgagc agggcggccc agaccatcgt caccgaggtg agcaccgcga gcaccgcgcg
      961 agcgggcggg tgctcggcat acagggggaa gagcgaggag acgacgacgg tgcccgcggc
     1021 gaccatcgtc gcaccgtgga tgagagccga ggcgggcgtc gggccctcca tggcgtcggg
     1081 gagccagtcg tggaagggca cgaggccgga cttgcccgcg acgcccatga gcagcaggac
     1141 cagtgcggtg gtcacggccg caccggtcgt cacgaggacc ttgctcagct gggtcgtgcc
     1201 cgcggcgacg gcgagcatga cgacgccgag gacgaacccg atgtcggcga agcgggtcac
     1261 gaggaaggcc ttgacggcag cccggcgagc ggactccttc gcggtgaggt ggccgatgag
     1321 caggtaggag ccccagccca tgacctccca cccgatcaga gtgagcacga ggtccccgga
     1381 catgacgacg aggagcatcg cggcggtgaa catcgagacg gtggcggcga acgcgccata
     1441 cctcttgtcg tcggcgaggt accagatcga gtagagctgc acgcccgctc cgacgatggc
     1501 cacgacggtc gcactggcgg cgttggcgtg cgtgacgagc aggttcagcg gtatggcgag
     1561 cgcgccggcg gcgagggagc cgat
//
LOCUS       DATAIX010000142         2228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_241920, whole genome shotgun sequence.
ACCESSION   DATAIX010000142 DATAIX010000000
VERSION     DATAIX010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241920"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /gene="cbiB"
                     /locus_tag="VHM65_02145"
     CDS             <1..658
                     /gene="cbiB"
                     /locus_tag="VHM65_02145"
                     /inference="COORDINATES: protein motif:HMM:NF015164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HEX2704532.1"
                     /translation="DMAALLDSGDLPAARHRLSHLCARDATGLNRDEIARAALESIAE
                     NTSDAMVAPLLWVAVAGVPGALGYRAANTLDAMVGYRSARYERFGWAPARLDDLLNAV
                     PARVGAALTAGCAPHVGGRAAMSWRVWRRDAAGHPSPNAGQVEAAFAGALGVTLGGAS
                     TYAGVPEVRGRLGDGPTPSTADLRRGVRLSRAVGLAATLASAAVAGAVTRIRPGRGRR
                     "
     gene            655..2142
                     /locus_tag="VHM65_02150"
     CDS             655..2142
                     /locus_tag="VHM65_02150"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEX2704533.1"
                     /translation="MTQRARGLLVAGTASDAGKSLVTAGICRWLARQGISVAPFKAQN
                     MSNNSMVCPDGAEIGRAQWVQALAAGITPEAALNPVLLKPGSDLRSHVVLMGQPWGDL
                     GSGEWASARRELAQAAYSAFRDLASRYDVVVAEGAGSPAEINLREGDYVNLGLAREMD
                     LPMVVVGDIDRGGVLAAMYGTLAILDPADQAVIAGWVVNKFRGHLGLLEPGLRMLEER
                     TGRPVLGVLPYLPGVWLDSEDSLALAHLPQARPPGPGRLSVAVVRLPRVSNATDIDAV
                     AGEPGVEVVVTTDPRVVAGADLAVIPGSRSTLADLAWLRRTGLADAVAARAAQGRAVL
                     GICGGYQMLAGTIRDPDGVEGSPGAVAEGLGLLPVEVTFAVEKALGTPSTTWRGQPVS
                     GYLIHHGRSTIRWDVTPPGGGARPFLDGVEAGAVFGTMWHGAFECDGFRRAFLAGVSE
                     DAGTGWAPDPGAPAFAQARERMLDTLADAIAEHLDTVALARIIGL"
BASE COUNT          257 a          805 c          847 g          319 t
ORIGIN      
        1 ggacatggcg gcgttgctcg actcggggga cctgcccgcg gcacgccacc ggctgagcca
       61 cctctgcgcc cgcgacgcca ccgggctgaa ccgtgacgag atcgcccgcg ccgcgctgga
      121 gtccatcgcc gagaacacct ccgacgcgat ggtggccccg ctgctgtggg tcgccgtcgc
      181 cggggtgccc ggtgcactgg gctatcgcgc cgccaacacc ctggacgcga tggtcggcta
      241 ccgctcggcc cgctatgagc gcttcgggtg ggcgccggcc cgcctcgacg acctgctcaa
      301 cgccgtgccc gcgcgggtcg gggccgccct gacggcgggg tgcgccccgc atgtcggcgg
      361 gcgcgcggcc atgtcctggc gggtctggcg ccgggacgcc gccggccatc cgagccctaa
      421 cgcaggccag gtcgaggccg ccttcgcggg cgctctcggt gtcacgctcg gcggtgccag
      481 cacctatgcc ggcgtccccg aggtgcgggg ccgccttggt gacgggccca cgccgagcac
      541 cgccgacctg cgcagggggg tgcgcctctc ccgtgcggtc gggctggccg cgaccctggc
      601 gtccgccgcg gtggcggggg cggtcacgcg catccggccg gggcgtggtc gacggtgacc
      661 cagcgggccc gcggtcttct cgtcgccggg acggcctccg acgccggcaa gtcgctcgtc
      721 accgccggga tctgccgctg gctggcccgt caagggatca gcgtcgcgcc cttcaaggcc
      781 cagaacatgt ccaacaactc gatggtctgc ccagacggcg ccgagatcgg ccgcgcccag
      841 tgggtccagg ccctcgccgc cggcatcact cccgaagccg ccctcaaccc cgtcctgctc
      901 aagccgggca gcgacctgcg ctcgcatgtc gtcctcatgg gacagccgtg gggcgacctc
      961 ggctcggggg agtgggcgag cgcacgacgt gagctcgcgc aggccgccta ctcggccttc
     1021 cgggacctcg cgagccggta tgacgtcgtc gtcgccgagg gcgcggggag cccggccgag
     1081 atcaacctgc gcgagggcga ctacgtcaac cttggcctcg cccgcgagat ggacctgccc
     1141 atggtggtcg tcggcgacat cgaccggggc ggggtcctgg ccgcgatgta cggcacgctg
     1201 gcgatcctgg accccgccga ccaggcggtc attgccgggt gggtcgtcaa caagttccgc
     1261 gggcacctgg ggctgctcga gcccgggctg cggatgctcg aggagcggac cggccggcct
     1321 gtcctcggcg tgctgcccta tctgccgggt gtgtggctgg actccgagga ctcgctggcc
     1381 ctcgcgcacc tgccgcaagc tcggccaccc gggcccgggc ggctcagtgt cgcggtcgtc
     1441 cggctgcccc gcgtcagcaa tgccacggac atcgacgccg tggccggcga gcccggggtc
     1501 gaggtcgtcg tgaccaccga cccgcgggtg gtcgcggggg ccgacctggc cgtcataccg
     1561 ggcagtcggt ccaccctcgc ggatctcgcg tggctgcgcc gtaccggcct cgctgatgcc
     1621 gtcgccgcgc gcgctgccca ggggcgggcg gtgctcggga tctgcggcgg ctaccagatg
     1681 ctcgccggga cgatcaggga cccggatggc gtcgaagggt cgccgggtgc ggtcgccgag
     1741 ggcttgggtc tgctgccggt cgaggtgacc ttcgccgtgg agaaggcact ggggacgccg
     1801 tcgacgacgt ggcggggtca gcccgtgagc ggctacctca tccaccacgg ccgttcgacg
     1861 attcgctggg acgtcacgcc cccggggggt ggtgcgaggc cgttcctcga cggggtcgag
     1921 gccggcgccg tgttcgggac gatgtggcat ggggccttcg agtgcgatgg tttccgcaga
     1981 gccttcctgg cgggggtgag cgaggacgcg ggtaccggct gggcgccgga tcctggcgcg
     2041 ccggccttcg cgcaggcccg ggaacggatg ctcgacacgc tggccgacgc catcgccgag
     2101 cacctcgaca cggtggcgct cgcacgcatc atcgggctct gagccggctc cggggtgcgg
     2161 gcacctaggg tggtcctatg agtaccgcat ggacgtggga gtatctcgac ccggccggcc
     2221 tgccgatg
//
LOCUS       DATAIX010000143         1959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_157304, whole genome shotgun sequence.
ACCESSION   DATAIX010000143 DATAIX010000000
VERSION     DATAIX010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157304"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VHM65_02155"
     CDS             <1..730
                     /locus_tag="VHM65_02155"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704534.1"
                     /translation="ARSKGTIVLVHLYPREMSIYGDLGNTRCLATRIERHGYAVDLRN
                     HHPGEPFPTDGQLFMGGGGQDSGQARVEDDLTRNGAELHAMARDGIPMLMICGMYQLF
                     GNAFVTVEGQELPGLGILDVTTQGNVRRMIGRVVVDTAHGLVVGYENHSGATELASGQ
                     KAFGVVRVGHGNNGADHSEGAVIHHVIGSYLHGPLLPANPAVADWLIAQAAERATGRP
                     FDPGELDDSLATEARTRQIRRLMR"
     gene            complement(848..>1959)
                     /locus_tag="VHM65_02160"
     CDS             complement(848..>1959)
                     /locus_tag="VHM65_02160"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX2704535.1"
                     /translation="PGVLGHDLAEPVPGGTPIRRMIQFVVGVGLALGLLGWALPHFAK
                     TSWGDVGRVLIAVPRPTAIGLLLLMLGGLWLYTFTFTGSLPGLSHGKAMIVNICGSSV
                     GNLLPGGGAAGVAVTYSQLRSWGFSRRDISTSIIVTGVWNVLARLALPLLGTIFMIFG
                     AGQLNPAVLRAALAGAVGGLGLLALFIAVLASDRAANVVGRVMERMVRPLRTWRARRW
                     AKREGMALDTTHPTAGLDGLITDLRARTIVVVRHGWVSMTCGMVGFFGMFYMLFWFCL
                     NSTGVQLPFAYMFFAYTLSRLLTAVGVTPGGLGVTETATAALLVGWGADPAQATAGVL
                     IFTLFTHILEVPLGAIGWVFWSASRKTPADGSAEG"
BASE COUNT          340 a          689 c          687 g          243 t
ORIGIN      
        1 cgcgcggtcg aagggcacga tcgtcctcgt ccacctctac ccgcgggaga tgtcgatcta
       61 cggcgacctc ggcaacaccc gctgtctcgc gacccggatc gagcgtcacg gctacgcggt
      121 cgacctgcgc aaccatcacc cgggggagcc cttcccgacc gacgggcagc tcttcatggg
      181 tggcggcggg caggactctg gccaggcgcg ggtcgaggac gacctcaccc gcaacggcgc
      241 agagctgcac gccatggcgc gtgacggcat accgatgctc atgatctgcg gcatgtacca
      301 gctcttcggc aacgccttcg tcaccgtcga gggccaggag ctgcccggcc tgggcattct
      361 cgacgtgacg acgcagggca acgtcagacg catgatcgga cgtgtcgtcg tcgacaccgc
      421 ccatgggctc gtcgtgggct acgagaacca ctcgggggcc accgagctgg cctcgggcca
      481 gaaggcgttc ggagtggtgc gcgtcgggca cggcaacaac ggcgccgacc acagcgaagg
      541 tgccgtcatc catcacgtca tcggctccta cctgcatggg ccgctcctgc cggccaaccc
      601 ggccgtcgcc gactggctca tcgcccaggc cgccgaacgg gcgaccgggc gaccgttcga
      661 tccgggcgag ctcgacgact ccctggccac cgaggcccgg acccgccaga tccgccggct
      721 catgcgatag gcgcggcccg tgcgagcatc ctcgcgccgg atagtggccc tgcgcgccgg
      781 agatgtgcgt cgcgaggagc agctcagcgg cgcgcggatg cgatggcgtg gacgggccgg
      841 cccccaccta accctcggcg gagccgtcgg caggggtctt cctgctggcg ctccagaaga
      901 cccagccgat cgcgccgagc ggcacctcga gaatgtgcgt gaagagggtg aagatcagca
      961 cgcccgcggt ggcctgggcg gggtcggcac cccagccgac gagcagcgcg gcggtggccg
     1021 tctcggtgac gccgagaccc cccggggtga caccgacggc cgtgagcagc cggctcaacg
     1081 tgtaggcgaa gaacatgtag gcgaagggga gctggacccc cgtgctgttc aggcagaacc
     1141 agaacagcat gtagaacatc ccgaagaagc cgaccatccc gcacgtcatg ctgacccacc
     1201 cgtgccggac gacgacgatc gtgcgagccc gcagatccgt gatgaggccg tcgaggccgg
     1261 ccgtcgggtg ggtcgtgtcc agggccatgc cctcgcgctt cgcccatcgc cgggcccgcc
     1321 atgtccgcaa cggccggacc atgcgctcca tcacccgtcc gacgacgttc gcggctcgat
     1381 ccgaggcgag gacggcgatg aacagtgcga gcagtccgag gccacccact gcgcccgcca
     1441 gggcagcacg gaggactgcc gggttgagct ggcccgcgcc gaagatcatg aagatcgtgc
     1501 cgagcagggg caatgccagg cgggcgagca cgttccagac gccggtcacg atgatcgagg
     1561 tcgagatgtc gcgccgcgag aagccccacg accgcagctg ggagtaggtg acggcgaccc
     1621 cggccgcgcc gccgccgggg agcaggttgc ccaccgagga cccgcagatg ttgacgatca
     1681 tcgccttgcc gtgcgagagc ccgggcagcg acccggtgaa cgtgaaggtg tagagccata
     1741 ggccgccgag catgagcagg agcagcccga tcgccgtcgg gcgaggcacg gcgatgagga
     1801 cgcgccccac atcaccccag ctcgtcttgg caaagtgggg cagcgcccag ccgagcaacc
     1861 cgagcgccag ccccaccccg acgacgaact ggatcatgcg ccggatcggg gtcccgccgg
     1921 ggacgggttc tgccaggtcg tgaccgagga cccccgggg
//
LOCUS       DATAIX010000144         1700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_37988, whole genome shotgun sequence.
ACCESSION   DATAIX010000144 DATAIX010000000
VERSION     DATAIX010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37988"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VHM65_02165"
     CDS             <1..474
                     /locus_tag="VHM65_02165"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEX2704536.1"
                     /translation="LMAKATSITDLASLEGELSRRQTELEAMQSQLKALEDQVAMSSV
                     TVTLAGPGTTLRDDTDWGFLNGLRNGLGALGNALVIGLTVLGAVLPFAAVLALLAAPL
                     IWWWRRRSPGRTPPAASPSGSSGPAAPVLSPPAPAPVSAGEEPAKGEAPRRPPLP"
     gene            complement(705..>1700)
                     /gene="hemE"
                     /locus_tag="VHM65_02170"
     CDS             complement(705..>1700)
                     /gene="hemE"
                     /locus_tag="VHM65_02170"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX2704537.1"
                     /translation="RRQRVERTPVWFMRQAGRSLPEYRALREGIGMLESCRRPDLVTE
                     ITLQPVRRHGVDAAILFSDIVVPLAAVGLDVEIVAGVGPVVHEPFRSLADLDRLPPLT
                     PEHVPDITEAVRLLTRELAGTPLIGFAGAPFTLASYLVEGGPSKGHERTKALMHGDPA
                     LWDALCARLAQISGAFLKVQVDAGASVVQLFDSWAGALSRADYVRFVRAHSAAALATV
                     AGTGVPRIHFGVGTGELLADIGAAGAEVVGVDFRVALADASARVGPGYALQGNLDPAL
                     LFAPWPVLAERVRQIVIEGAALPGHIFNLGHGVPPGTDPDALTRVVELVRESSAR"
BASE COUNT          259 a          634 c          605 g          202 t
ORIGIN      
        1 ctcatggcca aggcgacaag catcaccgac ctcgcttcgc tcgagggcga gctcagccgg
       61 cgccagaccg agctcgaggc gatgcagtcc cagctcaagg cgctcgagga ccaggtggcg
      121 atgtcctcgg tcaccgtgac cctggcgggg cctgggacga cgctgcgcga cgacacggac
      181 tggggcttcc tcaacggcct caggaacggt ctgggagccc tcggcaacgc cctggtcatc
      241 ggactcaccg tgctcggcgc ggtgcttccg ttcgcggccg tactcgcgct gctggctgcc
      301 ccgctcatct ggtggtggcg ccgccgctcg ccgggccgga ccccaccggc cgcgagtccc
      361 tcggggtcct ccggccctgc ggcacccgtc ctctccccgc ccgcaccggc acccgtctcg
      421 gctggcgagg agcccgcaaa gggcgaggca ccacgacgtc cgccgctccc gtgagatgcg
      481 tcatctcacc atttgcggca tctcacatgg tgcgccccgg cgtgccggct cgggcagccg
      541 acgcaccccg accggagtcg tatgccgtgc ccgccaccag cgggcacggc atggggcagc
      601 cgacgcaccg cgagcggagt cctatgccgc gcccgccacc agcgggcacg gcatactccg
      661 cgaaatggag cagtgccccc ggccgcgagc cagcgctgcc gcgctcagcg ggccgagctc
      721 tcccggacca gctcgacgac ccgggtgagc gcatccgggt cggtacccgg cggcaccccg
      781 tggccgaggt tgaagatgtg gcccggaaga gccgcgccct cgatcacgat ctggcgcacg
      841 cgctcggcca gcacaggcca gggcgcgaaa agcagggccg ggtcgaggtt gccctgcagg
      901 gcatagcccg gcccgacccg ggcggacgca tcggccagtg cgacccggaa gtccacaccg
      961 acgacctcgg ctccggccgc gccgatgtcg gcgagcagct cgccggtccc cacgccgaag
     1021 tggattcggg gcacaccggt gccggccacg gtggccagtg ccgcggccga gtgcgcccgc
     1081 acgaaacgca cgtagtcggc ccgggacagc gcaccggccc aggagtcgaa gagctgcacc
     1141 acggaggccc cggcgtcgac ctgcaccttg aggaacgcgc cggagatctg ggccagcctc
     1201 gcgcagaggg catcccacag cgcggggtcg ccatgcatga gggccttggt ccgctcgtgc
     1261 cccttggacg ggccgccttc gacgaggtat gacgcgagcg tgaagggagc cccagcaaac
     1321 ccgatgaggg gcgtccccgc gagctcgcgg gtgagcagcc gcaccgcctc ggtgatgtcg
     1381 gggacgtgct ccggcgtcag cggcgggagc cggtcgaggt cggccaggga ccggaacggc
     1441 tcgtggacca cgggcccgac cccggccacg atctcgacgt cgaggccgac ggccgcgagt
     1501 ggcacgacga tgtcgctgaa gaggatcgcc gcgtcgaccc cgtgacggcg caccggctga
     1561 agagtgatct cggtgacgag gtcgggccga cggcacgact ccagcatgcc gatcccctcg
     1621 cgcagagcgc ggtactcggg cagtgaccgc ccggcttgac gcatgaacca caccggggtc
     1681 cgctcgacgc gctggcgacg
//
LOCUS       DATAIX010000145         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_67985, whole genome shotgun sequence.
ACCESSION   DATAIX010000145 DATAIX010000000
VERSION     DATAIX010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67985"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VHM65_02175"
     CDS             <1..195
                     /locus_tag="VHM65_02175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2704538.1"
                     /translation="LEHRARSRGAAYLMLEVRADNASAIRVYERRGFDVATVRRRYYQ
                     PGDVDALVMRKTLQNKQAHG"
     gene            188..1234
                     /gene="tsaD"
                     /locus_tag="VHM65_02180"
     CDS             188..1234
                     /gene="tsaD"
                     /locus_tag="VHM65_02180"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX2704539.1"
                     /translation="MADQPLVLGIETSCDETGVGIVRGPTLLADAVASSVDEHARFGG
                     VVPEVASCAHLEAMVQTIERACREAGVRLADLEAIAVTAGPGLAGALMVGVAAAKALA
                     LALGKPVYGVNHLASHVAVDVLEHGPLPEPAMALLVSGGHSSILLVTDVTADISALGS
                     TIDDAAGEAFDKVARVLGLPFPGGPHIDRRARAGSSVAIDFPRGLTSGRDLERHRYDF
                     SFSGLKTAVARWVDARERSGEPVPAADVAASFQEAVVDVLTRKAVLACREYGVGDLLI
                     GGGVAANSRLRALARSRCDDAGIRLRVPRPGLCTDNGAMVAALGAEMVRRGRAPSALD
                     LPTDSSMPVTAVQA"
     gene            1295..>1601
                     /locus_tag="VHM65_02185"
     CDS             1295..>1601
                     /locus_tag="VHM65_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704540.1"
                     /translation="MSEQRPDVGVSRRLGALALAVVALAGCTSDPGAPAPTTTRGSST
                     STTARGLPSPASAASAPTPVALAAGVPTEAELGAARAEVGRLDIRELAGQLVVARYAG
                     "
BASE COUNT          193 a          566 c          611 g          231 t
ORIGIN      
        1 ctcgagcatc gcgcgcgcag ccgtggcgcg gcatacctca tgctcgaggt tcgcgcggac
       61 aacgcatcgg cgatccgggt ctacgagcgg cgcgggttcg acgtggccac agtgcggcgg
      121 cggtactacc agccgggcga cgtcgatgcc ctcgtgatgc gaaagacgtt gcagaacaag
      181 caggctcatg gctgaccagc ctctcgtgct tgggatcgag acgtcctgcg acgagaccgg
      241 cgtgggcatc gtccgcggcc cgaccctgct cgccgacgcg gtggcgagca gtgtcgacga
      301 acacgcacgc ttcggcggcg tcgtgcccga ggtcgcgagc tgcgcgcatc tggaagccat
      361 ggtgcagacg atcgagcggg cctgccgcga ggccggggtc cgcctggccg acctcgaggc
      421 gatcgcggtc accgccgggc cggggctggc cggcgcgctc atggtcgggg tcgccgccgc
      481 caaggccctc gccctcgccc tgggcaagcc ggtgtatggc gtcaaccacc tcgcgtcgca
      541 tgttgccgtt gacgtcctcg agcacgggcc tctgcccgag ccggccatgg cgctgctcgt
      601 ctcgggcggg cactcgtcca tcctcctggt gaccgacgtc accgcggaca tctcggcgct
      661 gggctcgacg atcgacgacg cggccgggga ggccttcgac aaggtggccc gggtgctcgg
      721 gttgcccttc ccgggcgggc cgcacatcga ccgacgggcc cgtgccggga gctcggtggc
      781 gatcgacttc ccccgcgggc tgacctccgg gcgcgacctg gagcggcatc gctacgactt
      841 ctccttcagc ggcctcaaga ccgcggtggc gcgctgggtc gacgcacggg agcgctcggg
      901 ggaaccggtt ccggccgccg atgtcgcggc gagctttcag gaggcggtcg tcgacgtgct
      961 gacccgtaag gccgtgctgg cctgccggga gtacggtgtc ggcgacctgc tcatcggcgg
     1021 gggagtcgcc gcgaactcgc ggctgcgggc cttggctcgg tcgcgctgcg acgacgcggg
     1081 gatccggctg cgggtcccgc ggccgggcct gtgcaccgac aacggcgcca tggtggccgc
     1141 cctcggagcc gagatggtcc gccgtggccg ggccccctcc gcgctcgacc tgccgacgga
     1201 ctcgtcgatg ccggtgacgg cggtccaggc ctagccttgg cctagcctcg gccctgctgg
     1261 gctgcctcgg ccgcgggtgg ggcagaatcg gtgggtgagc gagcagcggc cggacgtcgg
     1321 ggtgagccgg cgtctggggg cgctggccct ggccgttgtc gctctggccg gctgcacgag
     1381 cgacccgggc gcaccggcgc ccacgacgac acggggctcg tccacgtcca cgacagcacg
     1441 gggccttccg tcgccagcat cagccgcgtc ggctccgacc cccgtggcgc tggcggccgg
     1501 agtgccgact gaggccgagc tcggcgcagc ccgcgcggag gtgggccggc tcgacatacg
     1561 agagctcgct ggtcagctcg tcgtcgcgcg gtatgcaggg a
//
LOCUS       DATAIX010000146         2637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_241982, whole genome shotgun sequence.
ACCESSION   DATAIX010000146 DATAIX010000000
VERSION     DATAIX010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241982"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VHM65_02190"
     CDS             complement(<1..651)
                     /locus_tag="VHM65_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX2704541.1"
                     /translation="MPRRPTREPPKVALSSASVFPESAAATFDLAARLGYDGVEVMVW
                     TEPVSQEAGALRGLSRLHAIPVVSVHAPTLLLTQRVWGPGPWDKIDNAIRLALDLEAA
                     TVVVHPPFRWQSDYAAEFADGVAQREHDSGVQVAVENMYPWRAGQRELLAYAPHWDPV
                     EMPYDHVTLDLSHTASAGSDAMAMAEALGPRLAHVHLADGSGSALDEHLVPGRGSQP"
     gene            complement(651..1580)
                     /locus_tag="VHM65_02195"
     CDS             complement(651..1580)
                     /locus_tag="VHM65_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX2704542.1"
                     /translation="MRLGVIDVGSNTVHLLVVDAHAGAQPLPAFSHKIELRLSESMRD
                     DGSIAPSGAQRLVAFVRECLDVAAEQGVHEVLAFATSAIREAPNGDDVLALVQARTGV
                     TLTVLPGPDESRLTFLAVRRWFGWSSGQLLVVDIGGGSLELAIGLDEEPDVAVSLPLG
                     AGRLARSHLRHDPPTPAEVRTLRTLVRATIAQEVRAFSRGATVHRAVGTSKTMRSLAR
                     ILGAAPSSEGTYAARSLPLAQLSELVVRLGRMDHAARAGMPGVSMARAPQLLAGALVA
                     EAAMDLLGVASLEICPWALREGVILRRLDTLAS"
     gene            complement(1652..1828)
                     /locus_tag="VHM65_02200"
     CDS             complement(1652..1828)
                     /locus_tag="VHM65_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704543.1"
                     /translation="MTAFLVLLAVLAVPLGYVLRAQWRTTGSQIDSVIESVLAEPSPT
                     PLRALTPVRVPPRS"
     gene            2068..>2637
                     /locus_tag="VHM65_02205"
     CDS             2068..>2637
                     /locus_tag="VHM65_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704544.1"
                     /translation="MVETLLDIGRCWVEFTDPADEGRRFRADLTWLTSSWGCIFGKGC
                     RGLYVGRPDDGCCTLGAHFTDADDLERVAGAVALLTDAEWQYRAAVLPVPGDDPAEAT
                     TVTDPTRAWPKDEGDARRTRVVDGACVFLNRPGFPAGPGCALHHQAVLRGEPPHAVKP
                     DVCWQLPIRRQYREVTFPDDSTYLEVTITE"
BASE COUNT          410 a          887 c          976 g          364 t
ORIGIN      
        1 gggctggctc ccccggcccg ggaccagatg ctcatcgagc gcggagcccg atccgtcggc
       61 gaggtggacg tgggccaggc gtgggcccag cgcctcggcc atcgccatgg catccgaacc
      121 ggcgctggcg gtgtgggaca ggtcgagtgt gacgtggtcg tagggcatct cgaccgggtc
      181 ccagtgcggc gcgtaggcga gcagctcgcg ctgacccgcc cgccaggggt acatgttctc
      241 gacggcgacc tgcaccccac tgtcgtgttc acgctgggcc accccgtcgg cgaactcggc
      301 cgcatagtca ctttgccatc ggaacggcgg gtgcacgacg accgtggccg cctcgaggtc
      361 cagggcgagg cggatggcgt tgtcgatctt gtcccacggg ccggggcccc agacccgctg
      421 ggtgagcagg agggtcggtg cgtggaccga gacgaccggg atggcgtgca gacggctcag
      481 cccgcgcagc gccccagcct cctgcgagac cggctcggtc cagaccatga cctcgacgcc
      541 gtcgtagccg aggcgggccg cgaggtcgaa ggtggcagca gcggactccg ggaataccga
      601 ggcgctcgac agggcgacct tcggcggctc gcgcgtcggt cggcgaggca tcacgaggcg
      661 agagtgtcga gacggcgcag gatgaccccc tcacgcagcg cccaggggca gatctcgagg
      721 cttgcgaccc cgagcaggtc catcgcggcc tcggcaacga gcgctccggc cagcagctgc
      781 ggcgcgcggg ccatggacac cccgggcatg cccgcgcgcg ccgcgtggtc catgcggccc
      841 agccggacca cgagctcgct gagctgggcg agcggcaggc tgcgcgcggc atacgtgccc
      901 tccgagcttg gcgccgcacc gaggatccgg gcgagggacc gcatcgtctt ggacgtgccg
      961 accgctcggt gcaccgtggc gccacgggag aaggcgcgca cctcctgggc gatcgtcgcg
     1021 cggaccaggg tgcgcagcgt gcggacctct gcgggcgttg gggggtcgtg gcgcaggtgg
     1081 gagcgggcaa gccgcccggc gccgagtggc aggctcaccg cgacgtccgg ttcctcgtcg
     1141 agcccaatgg cgagctcgag ggatccgccg ccgatgtcga cgacgagcag ctggccgctg
     1201 gaccacccga accaccggcg gaccgcgagg aaggtcagcc gggactcgtc cgggccggga
     1261 aggacggtaa gcgtgacgcc ggtacgggcc tgcaccagag ccagcacgtc gtcaccgttc
     1321 ggggcctcgc ggatggccga ggtggcgaag gcgaggacct cgtggacacc ctgctcggcc
     1381 gcgacatcga gacactcgcg cacgaaggcg acaagccgtt gggctccaga cggtgcgatc
     1441 gagccgtcgt cacgcatgga ctcgctgagg cggagctcga tcttgtggga gaaggcgggg
     1501 aggggctgcg cccccgcgtg cgcgtcgacg acgagcagat gcacggtgtt cgagccgaca
     1561 tcgatcacac cgaggcgcat gggatcacgc tatcggtgcg ccgccagcgg ttcgtgcaag
     1621 gtcaacgcga cccggggtga cccggcccgg gtcacgacct ggggggcacg cggacgggcg
     1681 tgagggcccg gagcggtgtt ggggacggct cagccaggac ggactcgatc acggagtcga
     1741 tctgcgagcc ggtcgtgcgc cattgggcac gcaggacata gccgaggggg acggcgagaa
     1801 ctgcgagcag taccaggaat gcggtcacgg cggacatggg ctccttcttc caggagcggt
     1861 gctctcctgt tgactcggac ggcgtccggg acaatccatg acgggccagc gagaacttga
     1921 ccaactcttt accggcgatg gtaaccgttc actatctgaa ggtcacgctg actgcaattt
     1981 tcatgagtat agcactgctt ccgtgtaaca gcgacgtagt gtcgaggcgc ggcacgctga
     2041 tgtgtctctg cagctagggt tctgttcgtg gttgagaccc tgctcgacat cggccgctgc
     2101 tgggtggagt tcaccgaccc ggccgacgag ggccggcgct tccgcgccga cctgacctgg
     2161 ctcacctcca gctggggctg catcttcggc aaggggtgcc gcgggctcta cgtgggacgg
     2221 cccgacgacg ggtgctgcac cctcggagcc cacttcaccg atgctgacga cctcgagcgg
     2281 gtcgcgggag ccgtcgccct gctcaccgac gccgagtggc agtaccgcgc ggccgttctc
     2341 cccgtcccgg gtgacgaccc agccgaggcg acgaccgtga ccgatccgac gcgggcgtgg
     2401 cccaaggacg agggcgacgc acgaaggacg cgggtcgtcg acggtgcctg cgtcttcctc
     2461 aaccggcccg gcttcccggc cggaccgggg tgcgcgctcc accaccaggc cgtactccgt
     2521 ggcgagccgc cgcacgcggt gaagcccgac gtgtgctggc agctgccgat ccggcgccag
     2581 taccgcgagg tcacgttccc ggacgactcc acctacctcg aggtcaccat caccgag
//
LOCUS       DATAIX010000147         2064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_68010, whole genome shotgun sequence.
ACCESSION   DATAIX010000147 DATAIX010000000
VERSION     DATAIX010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68010"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..963)
                     /locus_tag="VHM65_02210"
     CDS             complement(220..963)
                     /locus_tag="VHM65_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX2704545.1"
                     /translation="MKAFHGTEVLKGIDLDVNRGQVVCLLGPSGSGKTTFLRCINQLE
                     TIDGGRIWVDGDLMGYEDRAGVLHRLNDKRIAAQRRDIGMVFQRFNLFPHKTALENIM
                     EAPIQVKGEKKSEVEVRAREVLDRVGLKDKPAAYPAQLSGGQQQRVAIARALAMRPKL
                     MLFDEPTSALDPELVGEVLDVMRELAHEGMTMIVVTHEMTFARDVADEVVFIDGGVVV
                     EQGHPRDVITNPQHQRTKTFLRRILAEDE"
     gene            complement(1017..1967)
                     /locus_tag="VHM65_02215"
     CDS             complement(1017..1967)
                     /locus_tag="VHM65_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX2704546.1"
                     /translation="MTTDLADRPGTINARPVRHPWRWVAIVIIAILVAMLVSSFLTNP
                     KWDFSYAFQIMQQKSVIQGLWVGTILGTVGSMVLGIVIGVLLAIMRLSDNPVLRGVAF
                     IYTWFFRSMPRYVLLAIMAVGVGYLYNRFDIGFPFGQQLAHALGMQNDLTFVSVRWDD
                     ISSTIWVGILGLGLSEAAYMAEIARAGILSVDKGQAEAAQALGMAPGKTMRRVVLPQA
                     MRVIVPPTGNETIAMVKDTSLFIAASIITELYFQATFFGSRAFKIMPGFVAATLWYLI
                     VCSILMVGQSYLENYYGRGFGTTPQKKRAKEKMLSIEAGH"
BASE COUNT          340 a          640 c          719 g          365 t
ORIGIN      
        1 cgctggaggg tgtcctggag ccgcgtgagc tgggccgctc ggagctcgtc gaccgaccac
       61 gagagcgcgg ggtcggtcct gcgtgccgtc gtcgtcatgc tgacctccgc tggggcgcca
      121 ttgtcttacc gcgatcctgc cgtgaggggg tgctgcccgc aaggacagat gcgacacagt
      181 gacatagcta ctcgtcgaca tagctactcg tcgacgtggc tactcgtcct cggcgaggat
      241 ccggcgcagg aacgtcttgg tgcgctggtg ctgcgggttg gtgatgacgt ccctggggtg
      301 gccctgctcg acgacgacgc caccatcgat gaagacgacc tcgtcggcga catcgcgggc
      361 gaaggtcatc tcgtgggtga cgacgatcat cgtcatcccc tcgtgggcga gctcacgcat
      421 gacgtcgagg acctcgccga ccagctcggg gtccagggcc gaggtcggct cgtcgaagag
      481 catgagcttg ggccgcatgg ccagggcccg ggcgatcgcg acacgctgct gctgaccgcc
      541 ggagagctgc gcggggtagg ccgcgggctt gtccttgagg cccactcggt cgagcacctc
      601 gcgcgcgcgg acctcgacct cgctcttctt ctcgcccttg acctggatgg gcgcctccat
      661 gatgttctcg agggccgtct tgtgcgggaa gaggttgaac cgctggaaga ccatcccgat
      721 gtcccggcgc tgggcggcaa tccgcttgtc gttcagcctg tgcaggacgc cggcgcggtc
      781 ttcgtagccc atgaggtcgc cgtcgaccca gatccggccg ccgtcgatgg tctcgagctg
      841 gttgatacag cgcaggaagg tcgtcttgcc cgagccggag ggaccgagca ggcagacgac
      901 ctggccgcgg ttgacgtcga ggtcgatccc tttgagcacc tcggtgccgt ggaaggcttt
      961 catgacgttg acggcgtgga ccaggggctg acccggtgcc gaagggtccg gggtcatcag
     1021 tggcccgcct cgatgctcag catcttctct ttggccctct tcttctgcgg ggtcgtgccg
     1081 aacccgcgtc cgtagtagtt ctcgaggtat gactgcccca ccatgaggat cgagcagacg
     1141 atgaggtacc acagggtcgc ggcgacgaag ccgggcatga tcttgaatgc tctcgacccg
     1201 aagaacgttg cctggaagta gagctcggtg atgatcgagg cggcaatgaa cagcgacgtg
     1261 tccttgacca tggcaatcgt ctcgttgccg gtggggggca cgatgacccg catggcctgc
     1321 gggaggacga cccgtcgcat cgtcttgccc ggtgccatgc cgagcgcctg cgccgcctcg
     1381 gcctggccct tgtcgaccga aaggatgccg gcgcgggcga tctcggccat gtatgcggcc
     1441 tcggacagcc cgagcccgag gatgccgacc cagatggtgc tcgagatgtc gtcccagcgc
     1501 acggagacga aggtgaggtc gttctgcatc ccgagggcgt gggcgagctg ctggccgaac
     1561 gggaagccga tgtcgaaccg gttgtagaga tacccgaccc cgacggccat gatggcaagc
     1621 aggacgtagc gcggcatcga gcggaagaac cacgtgtaga tgaaggccac cccccgcagg
     1681 acggggttgt cggagagccg catgatcgcg aggagcacgc cgatgacgat tccgagcacc
     1741 atcgacccca cggtgcccag gatcgtcccg acccagagcc cctggatgac cgacttctgc
     1801 tgcatgatct ggaaggcgta ggagaagtcc cacttcgggt tggtcaggaa cgagctgacc
     1861 agcatcgcca cgaggatggc gatgatgacg atcgcaaccc aacgccaggg atgccggacc
     1921 gggcgggcgt tgatcgtgcc cggccggtcc gcgaggtccg tcgtcatacg gctgggttga
     1981 cggcgaagtc ggtgatcgcg ccgccggcgc acttccactt ggtgaggatc gccttgtacg
     2041 tgccgtcctt gtcgagctgc ttca
//
LOCUS       DATAIX010000148         1621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_68081, whole genome shotgun sequence.
ACCESSION   DATAIX010000148 DATAIX010000000
VERSION     DATAIX010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68081"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /gene="mgrA"
                     /locus_tag="VHM65_02220"
     CDS             complement(<1..916)
                     /gene="mgrA"
                     /locus_tag="VHM65_02220"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HEX2704547.1"
                     /translation="MTYRVDEYRGAADRYDRLPYRRCGRSGLDLPLISLGLWHNFGDD
                     VPLERQQATLRRAFDLGVTHFDLANNYGPPYGSAERNFGALFARDLRPYRDELVISSK
                     AGWDMWPGPYGQGGGSRKYLLASLDQSLARMGLDYVDIFYSHRFDETTPLEETMGALD
                     TAVRSGRALYAGISSYSGARTREAVRILRELGTPLLIHQPSYSMLNRWIEHDLLDVLG
                     DEGVGAIAFSPLAQGMLTNKYLDGIPEGSRAAAGKSLDPSLLSDAALAHVRALNAIAA
                     ERGQSLAQMALAWGLRDERVTSVLVGASS"
     regulatory      1026..1144
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00634"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class IV; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00634"
     gene            1233..>1621
                     /locus_tag="VHM65_02225"
     CDS             1233..>1621
                     /locus_tag="VHM65_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX2704548.1"
                     /translation="MSDTPSWSFETRQVHAGQIADATTGARALPIYQTTSYAFRDTEH
                     AANLFALKEFGNIYTRIMNPTQDAVEQRIASLEGGLAALLVASGQAAETIAFLNIAEA
                     GDHIVSSPSLYGGTYNLLHYTLPKYGLA"
BASE COUNT          256 a          579 c          555 g          231 t
ORIGIN      
        1 cgctggaggc gccgacgagg acggaggtga ctcgttcgtc gcgcagcccc caggccaggg
       61 ccatctgcgc caaggactgg ccgcgctcgg cggcgatcgc gttgagcgct cgcacgtggg
      121 ccagcgccgc gtcggagagc agcgaggggt ccagggactt gcccgcagcg gcccgcgagc
      181 cttcggggat gccgtcgagg tacttgttcg tcagcatccc ctgggccagc ggcgagaagg
      241 cgatggcgcc caccccctcg tcgccgagga cgtcgagcag gtcgtgctcg atccagcggt
      301 tgagcatcga gtaggacggc tggtggatca gcagcggcgt gcccagctcg cgcaggatgc
      361 gcaccgcctc gcgggtccgc gcacccgagt aggacgagat accggcatac agcgcccgac
      421 cggaccgcac ggcggtgtcc agcgcaccca tcgtctcctc gagcggtgtg gtctcgtcga
      481 aacggtggct gtagaagatg tcgacgtagt ccaggcccat ccgcgccagg gactggtcca
      541 gggaggcgag caggtacttg cgcgacccgc cgccctgccc gtaggggccc ggccacatgt
      601 cccagcccgc cttgctcgag atgacgagct cgtcccggta ggggcgcagg tcgcgggcga
      661 acagcgcccc gaagttgcgc tctgcgctgc catagggcgg gccgtagttg ttcgccaggt
      721 cgaagtgcgt cacgccgagg tcgaaggccc ggcgcagcgt ggcctgctgg cgctcgaggg
      781 gcacgtcgtc gccgaagttg tgccagagcc ccagggagat gagcggcagg tccagcccgc
      841 ttcggccgca ccggcggtag gggaggcggt cgtatctgtc tgcggcgccg cggtactcgt
      901 cgacgcggta ggtcatgacc ccatcctgcc tccgccgtcg ccgccctgtt ctcccccagg
      961 tcggccgtcc cagatgaaga gacacgcctt caggtggcgt tgacgccgtg gttacgctct
     1021 agccaggtca tgagtgccag cgcacagccc cggcttgctg gtcggcaacc ctccaccgcg
     1081 gtggggtgcc ccgggtgaag acctggccac cgcgctctct cgggctcggc ggcaagcgcg
     1141 gaggtcaccc ggctctccgc atcgtcacct cggcgcacct ggtgcagggg tgacggcacc
     1201 ggcatacgag cagatgtgaa ggaacaacca ccatgagcga cacccccagc tggtccttcg
     1261 agacccgcca ggtccacgcc ggccagatcg cggacgccac gaccggcgcc cgcgcactgc
     1321 cgatctacca gacgacctcc tacgccttcc gggacaccga gcacgcggcg aacctgttcg
     1381 cgctcaagga gttcggcaac atctacaccc ggatcatgaa ccccacgcag gacgccgtcg
     1441 agcagcggat cgcctcgctc gagggaggcc tggccgcgct gctcgtcgcc tccggccagg
     1501 cggctgagac catcgcgttc ctcaacatcg ccgaggccgg cgaccacatc gtctcgagcc
     1561 cgagcctcta cggcgggacg tacaacctcc tgcactacac gctgcccaag tacggcctgg
     1621 c
//
LOCUS       DATAIX010000149         2808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_97771, whole genome shotgun sequence.
ACCESSION   DATAIX010000149 DATAIX010000000
VERSION     DATAIX010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97771"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2350)
                     /locus_tag="VHM65_02230"
     CDS             complement(<1..2350)
                     /locus_tag="VHM65_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704549.1"
                     /translation="MLSFFDSITVPGLEKVTVYRDDEDAYRFYVMPSTPRIARDDSGE
                     LLLQLMIYARNVDTLPPDQLEAQRGWIAASVELRLTPEEEDKVRAYLKELINAGNHLW
                     FARFFRLRLDPAEPKITLPPEWLSGDVALSIPTPGGTSTVSTSKPSLISTNVATLAGD
                     LSQDSSELLRQAVLKGGLPMAVNYTDLTFVARIPSITVRIHGDRSAFLRETIERYQIT
                     HTIVHREEVDLGLISWTTEWWQQWTTNHTSVDQFQQITQSITLEVEDSDFRTEPEYVA
                     IRAKFEAMALEIFTASVVPSIMADVSKAFDAARTAAAGDPAKPAPPCSVQTLVSTISG
                     SVDMTLSKSAVVQLKKNPNGALARDLTPEQITKAVTYIDLSDPYFRELFLHVHANVNF
                     ATDPVFGLKVNVEYEQQDEQMTRVVKGAKSMLFTSADQVQTFRQILARGADGATKDEY
                     RYWSEIIYKETGQTIRVPATGTLPGKGNELVISYRSLGFVKVLATLGPQPAEVASVKV
                     SFSYPRSAAATATQSFELTTANPTATYFTYAGHPGEPEPYTYTLTYVLTDGQQMDVPP
                     ATSRGATLAVGSPFESRVSATFVAQADFTVVDKIILDATYSDPANDLTLNHHAELAGN
                     GASDAWSVALRNPALTGFTYSTLVLYKNGSSEQRGPTSATLGTTVPVGVGATAALEVM
                     LVPNLDATRPTAVVQMTYDDPAAGVHQAQNYTMGMTDAPATFRVLLRNPEARAYRYRI
                     QLLATASAPVWDSGWRDGTDTVLLVRADQAPPPPPTPAPPPPT"
     gene            complement(2400..>2808)
                     /locus_tag="VHM65_02235"
     CDS             complement(2400..>2808)
                     /locus_tag="VHM65_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704550.1"
                     /translation="VPTAAGLAGVVVELAYADPGGDPAFAQSDSLFFDDLGAGTPQEW
                     RARLKDRAARGYTWALTTLKNDGTQTTTTPERATQESLFIRPPIAGAPVTPAPVTPAP
                     VTPALVTPALVTPTPVTPGPAPAPAPGPDDGRP"
BASE COUNT          370 a          861 c         1091 g          486 t
ORIGIN      
        1 gtgtcggtgg tggcggagcc ggtgtgggtg gcggcggcgg cgcctggtcg gcccggacga
       61 gtagcacggt gtccgtgccg tcgcgccagc cggagtccca gaccggcgcc gaggccgtcg
      121 cgagcagctg gatccgatag cggtaggccc gtgcctcggg gttgcgcaac aggacccgga
      181 acgtcgccgg cgcgtccgtc atacccatcg tgtagttctg ggcctggtgc acgcctgcgg
      241 ccgggtcgtc atacgtcatc tgcacgacgg cggtgggccg cgtggcgtcg aggttcggca
      301 cgagcatcac ctcgagcgcg gcggtcgcgc cgacgccgac cggcaccgtc gtgccgaggg
      361 ttgccgacgt cggcccacgc tgctcgctcg agccgttctt gtacaggacg agcgtcgagt
      421 aggtgaaccc ggtcagcgcg gggttgcgca gggcgaccga ccaggcgtcg gaggcgccgt
      481 tgcccgcgag ctcggcgtgg tggttgaggg tgaggtcgtt cgccgggtcg ctgtaggtcg
      541 cgtcgaggat gatcttgtcg acgacggtga agtcggcctg ggcgacgaag gtcgccgaga
      601 cccggctctc gaacgggctc ccgaccgcga gcgtcgcgcc ccggcttgtc gcgggcggca
      661 cgtccatctg ctgtccgtcg gtgagcacgt aggtcagcgt gtaggtgtac ggctcgggct
      721 ccccgggatg gccggcatag gtgaagtagg tcgccgtcgg gtttgccgtc gtcagctcga
      781 aggactgcgt cgccgtggcc gcggcggagc gcgggtagct gaacgacacc ttgaccgagg
      841 ccacctcggc gggctggggt cccagggtcg ccaggacctt gacgaacccg agcgaccggt
      901 aggagatgac gagctcgttg cccttgcccg gcagggtgcc ggtggcgggg acccggatcg
      961 tctggccggt ctccttgtag atgatctccg accagtagcg gtactcgtcc ttggtggcgc
     1021 cgtccgcccc gcgggccagg atctggcgga aggtctggac ctggtcggcg ctggtgaaga
     1081 gcatcgactt ggcgcccttg acgacccgcg tcatctgctc gtcctgctgc tcgtactcga
     1141 cgttgacctt gaggccgaag accgggtcgg tggcgaagtt gacgttggcg tgtacgtgca
     1201 ggaacagctc gcggaagtag gggtcggaca ggtcgatgta ggtgacggcc ttggtgatct
     1261 gctcgggggt gaggtcgcgg gcgagggccc cgttcgggtt cttcttgagc tggacgacgg
     1321 cgctcttgct cagcgtcatg tcgaccgacc cgctgatggt ggacacgagc gtctgcaccg
     1381 agcagggcgg ggccggcttg gccgggtcgc cggcggcagc ggtgcgggcc gcgtcgaacg
     1441 ccttgctgac gtcggccatg atgctcggca cgaccgaggc ggtgaagatc tccagcgcca
     1501 tggcctcgaa cttggcgcgg atggcgacgt actcgggctc ggtgcgaaag tccgagtcct
     1561 cgacctcgag ggtgatcgac tgcgtgatct gctggaactg gtcgaccgac gtgtggttcg
     1621 tcgtccactg ctgccaccac tcggtcgtcc acgagatcag gccgaggtcg acctcctcgc
     1681 gatgcacgat cgtgtgcgtg atctggtagc gctcgatcgt ctcgcggagg aaggcgctgc
     1741 ggtcgccgtg gatgcgcacg gtgatcgacg ggatgcgcgc gacgaaggtg aggtcggtgt
     1801 agttgaccgc catcggcagc ccacccttga ggacggcctg gcggagcagc tcgctcgagt
     1861 cctggctgag gtcgccggcg agggttgcca cgttggtgct gatgagtgac ggcttcgagg
     1921 tcgagaccgt gctcgtgccc cctggcgtcg ggatcgacag ggcgacgtcg ccggagagcc
     1981 actccggcgg cagggtgatc ttgggctccg ccgggtcgag ccgcagccgg aagaaccggg
     2041 cgaaccagag gtggttgccc gcgttgatga gctccttgag gtaggcgcgg accttgtcct
     2101 cctcctccgg ggtgaggcgc agctcgaccg aggcggcgat ccagccgcgt tgtgcctcga
     2161 gctggtccgg gggcagcgtg tcgacgttgc gcgcgtagat catgagctgc aggagcagct
     2221 ccccggagtc gtcgcgggcg atccgcggag tcgacggcat gacgtagaac cggtaggcgt
     2281 cctcgtcgtc ccggtagacc gtcaccttct ccagccctgg aacggtgatg ctgtcgaaga
     2341 agctcagcat ggtcgggtcc tcctgagtgg gtcgatgccg gtatgacggg tgcgcgggct
     2401 cacggtcgtc cgtcgtccgg tccgggtgcc ggggcgggcg cgggtcccgg tgtcacgggc
     2461 gtcggtgtca ccagcgccgg tgtcaccagc gccggtgtca cgggcgccgg tgtcacggga
     2521 gccggtgtca cgggagcgcc ggcgatgggt gggcggatga agaggctctc ctgcgtcgct
     2581 cgctcgggag tggtcgtcgt ctgggtgccg tcgttcttga gggtggtcag cgcccacgtg
     2641 tagccccgag cagctcggtc cttgagccgg gcccgccact cctgcggcgt ccctgcgccg
     2701 aggtcgtcga agaagagcga gtcgctctgg gcgaaggccg ggtcaccgcc cgggtcggcg
     2761 taggcgagct cgacgacgac acccgcgagt cccgccgcgg tcgggacg
//
LOCUS       DATAIX010000150         4137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_212180, whole genome shotgun sequence.
ACCESSION   DATAIX010000150 DATAIX010000000
VERSION     DATAIX010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212180"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VHM65_02240"
     CDS             complement(<1..833)
                     /locus_tag="VHM65_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704551.1"
                     /translation="MVTRLATGEGWGLLESLPPYDGATVLPLQARLRAAGFDADLVAA
                     VLTQSRLRARAVDKLGAFAAGMLFTDDGLEQATRLPLAARHAERFVTAGVATVHDLGC
                     GIGADAMAFAGLDLAIQAIDADPATAAVATVNLRHFPEATVRAGLAETVALPSGARAR
                     HTGVWVDPARRVTGVADSRGRTRRVFALDAISPPWTRVQEWAAQVPAAGAKLSPALAH
                     QAIPAGAEAEWVSWQGEVLECVVWFGSLARRPGRVASVCRRDRLPVSVAEADAHGGDA
                     TL"
     gene            864..2093
                     /locus_tag="VHM65_02245"
     CDS             864..2093
                     /locus_tag="VHM65_02245"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEX2704552.1"
                     /translation="MTPLSDSEHRAWLAAEAVRLVDFARESRHPDGGFAWLGSNGEPL
                     LAKPVELWITARMTHVFALGQLLGLPGCEELVDHGIDALNGRLHDDRHGGWFTAVGSS
                     APIITDKEAYGHAFVVLAATSANAAGHPDAHWLLADALGVLDERFWDEPYGMVVDRCD
                     ETWATTDPYRGVNANMHTVEAYLAASDVTGDPGWRDRALRITERVLGYGAAGSWRLPE
                     HFDTTWTPQLEYNRDHPADPFRPYGATVGHGLEWARLALALAEGLGEGAPATLRDGAV
                     ALFDRAVSDGWSVDGEPGLVYTTDWSGVPVVRQRMHWVAAEATATATVLHRATGDERY
                     ARWERLFWEHIRELFVDSAHGSWHHELDPANHPSASIWHGKPDVYHALQAVLLPRLPT
                     TPSFATALAQGRLDMPE"
     gene            2427..2744
                     /gene="groES"
                     /locus_tag="VHM65_02250"
     CDS             2427..2744
                     /gene="groES"
                     /locus_tag="VHM65_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX2704553.1"
                     /translation="MPEGEVTTVSVSITPLEDRIVIKSLEAETTTASGLVIPDTAKEK
                     PQEGEVLAVGPGRISDKGNRIPLDIKVGDRVIYSKYGGTEIKHKGEEYLILSARDVLA
                     VVG"
     gene            3011..>4137
                     /gene="groL"
                     /locus_tag="VHM65_02255"
     CDS             3011..>4137
                     /gene="groL"
                     /locus_tag="VHM65_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX2704554.1"
                     /translation="MAKTLEFNDSARKSLERGVDALANAVKVTLGPKGRNVVIDKKWG
                     APTITNDGVTIAREIELEDSYENLGAQLAKEVATKTNDVAGDGTTTATVLAQAMVKEG
                     LRNVAAGAAPSALKRGMDLAVEAVNERLLANARELGGHDEVAQVATLSAQDDAIGTLI
                     ANAFDKVGKDGVITVEESSTTATELEFSEGMQFDKGYISAYFVTDAERMESVLEDAYI
                     LIHQSKISAISDLLPILEKVVQSGKPLLIVAEDVDGEALSTLVVNKIRGTFTVTAVKA
                     PGFGDRRKAMLQDLAILTGGQVISEEVGLKLEQATLDLLGQARRVVVTKDNTTVIDGA
                     GSSDEVAARVHQLKAEIERTDSDWDREKLQERLAKLAGGVCV"
BASE COUNT          645 a         1504 c         1429 g          559 t
ORIGIN      
        1 agcgtggcat cgccgccgtg cgcgtcggcc tcggcgacgc tgaccggcag ccggtcgcgg
       61 cggcataccg aggccacccg gccgggccgg cgtgcgagcg agccgaacca caccacgcac
      121 tcgaggacct cgccctgcca cgagacccac tccgcctcgg cgcctgccgg gatcgcctgg
      181 tgtgccaagg cggggctcag cttggctccg gccgcgggga cctgggcggc ccactcctgg
      241 acccgggtcc agggcggcga gatcgcgtcg agggcgaaga cccgcctcgt ccgcccgcgc
      301 gagtccgcga ctccggtgac gcgccgggcg gggtcgaccc agacgccggt atggcgcgcc
      361 cgggcgccgc tgggtagggc gaccgtctcg gcgagcccgg cccggacggt ggcctcgggg
      421 aagtggcgca ggttgacggt ggcgaccgcc gcggtggccg ggtcggcgtc gatggcctgg
      481 atggcgaggt cgagcccggc gaacgccatg gcgtcggcgc cgatcccaca gccgaggtcg
      541 tgcacggtcg caaccccggc ggtgacgaag cgctcggcgt gccgggcggc cagcggcagc
      601 cgggtcgctt gctcgaggcc gtcgtcggtg aagagcatcc cggccgcaaa cgcgccgagc
      661 ttgtcgaccg cccgcgcccg cagccggctc tgggtcaaca cggcggcgac gaggtcggcg
      721 tcgaagcccg ccgcccgcag ccgtgcctgc agcgggagga ccgtggcgcc gtcatacggg
      781 ggcagggact cgagcagccc ccagccctcg ccggtggcga gccgagttac catcgccacg
      841 tccacgagtg acatcctgtc ctagtgaccc ccctctccga ctccgagcac cgggcgtggc
      901 ttgcggcgga ggccgtgcgc ctcgtcgact tcgcccgtga gtcgcgccac cccgacggag
      961 ggttcgcctg gctcgggtcc aacggtgagc ccttgctggc caaaccggtc gagctgtgga
     1021 tcaccgcccg gatgacccac gtcttcgccc tcggccagct gctcggcctg cccgggtgcg
     1081 aggaactggt cgaccacggg atcgacgcgc tgaacggtcg gctgcacgac gaccgtcacg
     1141 gcggctggtt caccgccgtc gggtcctccg cgccgatcat caccgacaag gaggcctacg
     1201 gacacgcctt cgtcgtcctc gctgccacgt cggcgaacgc cgcgggtcat ccggacgcgc
     1261 actggctgct cgccgacgcg ctgggggtgc tcgacgagcg gttctgggac gagccctacg
     1321 ggatggtcgt cgaccgctgc gacgagacat gggcgacgac ggacccttac cgcggggtca
     1381 acgccaacat gcatacggtc gaggcctacc tcgcggcatc ggatgtcacc ggtgaccccg
     1441 gctggcgcga tcgtgccctg cggatcaccg aacgcgtcct cgggtatggc gccgccggct
     1501 cgtggcggct gccggagcat ttcgacacca cgtggacgcc gcagctggag tacaaccgcg
     1561 accatccggc cgacccgttc cgcccctatg gtgcgaccgt gggccacggg ctggaatggg
     1621 cccggctcgc gctggcgctc gccgaggggc tgggtgaggg cgcccctgcg acgctgcgcg
     1681 acggggcggt ggcactgttc gaccgggccg tgagcgacgg gtggtcggtc gacggggagc
     1741 ccggactggt ctataccacc gactggtctg gtgtgccagt ggtccgccag cggatgcact
     1801 gggtcgccgc cgaggccacc gccacggcca cggtgctgca ccgggccacc ggtgacgagc
     1861 ggtatgcccg gtgggagcgc ctgttctggg agcacatccg tgagctgttc gtcgactcgg
     1921 cccacgggtc ctggcaccac gagctcgacc ccgcaaacca cccgtcggcg agcatctggc
     1981 acggcaagcc cgatgtctac cacgccctgc aggccgtgct gctgccccgc ctgccgacca
     2041 ccccgtcctt cgccacggcg ttggcccagg gccgcctcga catgccggag taatctggtc
     2101 caaccgaggc gccacccctc cgtggacaag acagcgcgcc tcgctggccc atctgcccac
     2161 agacctcctc ccgggccgcg cctgttccac ctgcccgacc ggcacggcaa ccgagtcggg
     2221 cgtggtgccg ccggagtgag ggtggcgccc acctgctgcg acacccccgg ttagcactcg
     2281 gcttgaccga gtgctaaccg gcgacttaga ttctgtggcg ttagcactct gcctagctca
     2341 gtgccaacgg cactcccgca gtgcgccgac ccccgcgacg gcggcgtccg tgcagagtgc
     2401 atccaagaca tctgtccaac ttcgacattc ccgaagggga ggtcaccacc gtgtcggttt
     2461 ccatcacgcc gctcgaagac cgcatcgtca tcaagtccct cgaagccgag accacgacgg
     2521 cgtcaggcct ggtcatcccg gacaccgcga aggagaagcc ccaggagggc gaggtcctcg
     2581 cggtcggccc gggtcgcatc agcgacaagg gcaaccggat cccgctcgac atcaaggtcg
     2641 gcgaccgggt catctacagc aagtacggcg gcaccgagat caagcacaag ggcgaggagt
     2701 acctcatcct gtccgcccgc gacgtcctcg cggtcgtcgg ctgactgacc cgtgcgaatc
     2761 gagcttgctg ggtccccgcg ctctgcgcgg gtggcccgga gcgggtcgcg caagccggcc
     2821 acagggccgt cggctcacgc ccacaggccc aggcgctggt atgccgtgac cgcacctcgc
     2881 gcgggtacct gggtcccagg ccctgcgccg gggtgtgctc gggccgccct gccgcccacg
     2941 tcttactgaa ccacccacca gctgggacgc gacccacgag gcgcgcgccc ggcatacgag
     3001 aaggaagctc atggccaaga cgctggagtt caacgactcc gcccgcaagt cgctcgagcg
     3061 tggcgtcgac gcgctcgcca acgcggtcaa ggtgacgctc ggtcccaagg gccgcaacgt
     3121 cgtcatcgac aagaagtggg gcgcgccgac gatcaccaac gacggcgtga cgatcgcccg
     3181 cgagatcgag ctcgaggact cctacgagaa cctcggcgcg cagcttgcca aggaggtcgc
     3241 gaccaagacc aacgacgtcg ccggtgacgg gacgaccacc gcaacggtcc tcgcccaggc
     3301 gatggtcaag gaaggcctgc gcaacgtcgc cgcgggcgcc gccccgtccg cgctcaagcg
     3361 cgggatggac ctggcggtcg aggcggtcaa cgaacggctc ctcgccaacg cgcgtgagct
     3421 cggcgggcac gacgaggtcg cccaggtcgc cacgctctcg gcgcaggacg acgcgatcgg
     3481 cacgctcatc gccaacgcgt tcgacaaggt cggcaaggac ggcgtcatca ccgtcgagga
     3541 gtcgtcgacg acggcgaccg agctcgaatt ctctgagggc atgcagttcg acaagggcta
     3601 catctcggcc tacttcgtca ccgacgcgga gcggatggag tcggtcctcg aggacgccta
     3661 catcctgatc catcagagca agatctcggc catctccgac ctgctcccga tcctggagaa
     3721 ggtcgtccag tcgggcaagc cgctgctcat cgtggccgag gacgtcgacg gcgaggcgct
     3781 ctccacgctg gtcgtcaaca agatccgggg caccttcacc gtcaccgcgg tcaaggcgcc
     3841 cggcttcggc gaccgccgca aggcgatgct gcaggacctc gcgatcctca cggggggtca
     3901 ggtcatctcc gaggaggtcg ggctcaagct cgagcaggcc accctcgacc tgctcggcca
     3961 ggcccgtcgc gtcgtcgtca ccaaggacaa cacgacggtc atcgacgggg cgggttccag
     4021 cgacgaggtc gccgcccggg tccaccagct caaggccgag atcgagcgaa ccgactccga
     4081 ctgggaccgc gagaagcttc aggagcgcct cgccaagctc gccggtgggg tctgcgt
//
LOCUS       DATAIX010000151         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_122862, whole genome shotgun sequence.
ACCESSION   DATAIX010000151 DATAIX010000000
VERSION     DATAIX010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122862"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VHM65_02260"
     CDS             complement(<1..736)
                     /locus_tag="VHM65_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2704555.1"
                     /translation="MTNLAGNLAATAGRYPDKVAIRLDQAELSWSQFYGLARAAAGAL
                     KAEGLEPGDRVALILPNVPSYPVLFYGTLLAGGIVVPMNPLLKRHEIEFFFADSGAKF
                     AFVWPDFVAEAQAGAENPGTRVIPTGPLGPVPGSFTADAAPVAAPAERDDTDTAVILY
                     TSGTTGKPKGAELTHANLHLNASRSATVIMAMTHDDVIMGCLPLFHVFGLTCGLNAAT
                     VAGATLTLIPRFDPAKALEVIGRDKVT"
     gene            889..>2301
                     /gene="ligA"
                     /locus_tag="VHM65_02265"
     CDS             889..>2301
                     /gene="ligA"
                     /locus_tag="VHM65_02265"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX2704556.1"
                     /translation="MIEHEPVIERSEPGVPADVRHTWTDLAEEARRHQFAYHVQDAPT
                     VSDGEYDALMRRLCALEDRWPELRTPDSPTQQVGGAAFSTDFAAVDHLERMMSLDNAF
                     SSEELVAWAARVAREAGDEVSYLCELKIDGLAVNLLYERGLLTRALTRGDGRTGEDVT
                     LNVRTISGVPTRLGGHARPDLVEVRGEVFFPTEAFSDLNAALVESGKAPFANPRNAAA
                     GSLRQKDPRVSASRPLRMLVHGIGARSGFEITRQSQGYDALRGWGLPVSAHSRVVESL
                     AAVEEFIAHFGEYRHSVEHEIDGVVVKVDEVALQRRLGSTSRAPRWAIAYKYPPEEVN
                     TRLLDIRVNVGRTGRVTPFGVMKPVVVSGSTVEMATLHNADEVRRKGVLIGDTVVLRK
                     AGDVIPEIVGPVVELRDGSEREFVMPTHCPSCGTALAPAKEGDKDIRCPNTRACPSQL
                     RERVFGLASRGALDIEALGWK"
BASE COUNT          321 a          758 c          872 g          350 t
ORIGIN      
        1 tcgtgacctt gtcgcgcccg atgacctcga gggccttggc cgggtcgaag cgcgggatga
       61 gggtcagcgt ggcgccggcc accgtcgccg cgttgagccc gcaggtgagg ccgaagacgt
      121 ggaagagcgg caggcagccc atgatgacgt cgtcgtgggt catcgccatg atcacggtcg
      181 ccgagcgcga ggcgttgagg tggaggttgg cgtgagtcag ctcggcgccc ttgggcttgc
      241 cggtcgtccc tgacgtgtag aggatgaccg ccgtgtcggt gtcgtcgcgc tcggcgggag
      301 cggccaccgg tgccgcgtcg gcggtgaacg aaccgggcac cgggccgagc gggccggtcg
      361 ggatgacccg ggtccccggg ttctcggcgc cggcctgggc ctcggccacg aaatcgggcc
      421 agacgaaggc gaacttggcc cccgagtcgg cgaagaagaa ctcgatctcg tgccgcttga
      481 gcagggggtt catggggacc acgatcccgc ccgccagcag ggtgccgtag aagagcacgg
      541 ggtatgacgg cacgttcggc aggatcagcg ccacccggtc gccgggctcc agtccctcgg
      601 ccttgagggc tcccgccgcg gcgcgggcga gcccgtagaa ctgggaccag ctcagctcgg
      661 cctggtcgag gcggatcgcg accttgtcgg gatagcggcc cgcggtggcg gcgaggttgc
      721 cggcgaggtt agtcatgagc gtgtgctcct tagctccgtt ggtccgtgga ttcggcccta
      781 ttcttaccgg ttggtagccc gctggccagg gcctcgtcgt cgcgcacggt ggccgtcctg
      841 tcggcggtct ggcgagggcc tgtcggggcg gtggtgcact atgggcccgt gatcgaacat
      901 gagcccgtga tcgagcgcag cgagcccggc gttcccgccg acgtccgcca tacctggacc
      961 gacctggccg aggaggctcg tcggcaccag ttcgcctacc acgtccagga cgctccgacg
     1021 gtcagtgacg gggagtacga cgcgctcatg cgccgcctct gcgccctgga ggaccggtgg
     1081 cccgagctgc ggacgccgga cagcccgacg cagcaggtgg ggggcgcggc gttctcgacc
     1141 gacttcgcgg cggtcgacca tctcgagcgg atgatgagcc tggacaacgc cttctccagc
     1201 gaggagctcg tggcgtgggc ggcccgggtg gcccgtgagg cgggtgacga ggtgagctac
     1261 ctgtgcgagc tcaagatcga cgggctcgcg gtcaacctgc tctacgagcg tggcctgctg
     1321 acccgggcgc tgacccgcgg cgacgggcgc acgggcgagg acgtcacgct caacgtccgc
     1381 acgatctccg gcgtcccgac ccgcctcggg ggccacgcca gaccggatct cgtcgaggtg
     1441 cggggggagg tgttcttccc cactgaggcc ttttccgacc tcaacgcggc gctcgtcgag
     1501 tccggcaagg cgccgttcgc caacccccgc aacgccgccg ccgggtcact gcgccagaag
     1561 gacccgcggg tcagcgcctc gcgcccgttg cggatgctcg tgcacgggat cggggcccgc
     1621 tcggggttcg agatcacccg ccagtcgcag gggtatgacg cgctgcgggg ctgggggcta
     1681 cccgtgtcgg cacattcgcg cgtcgtggag tcgctggcgg cggtggagga gttcatcgcc
     1741 cacttcggcg agtaccggca ctcggtcgag cacgagatcg acggggtggt cgtcaaggtc
     1801 gacgaggtgg ccctgcaacg ccggctgggg tcgacctcgc gggcgccgcg gtgggcgatc
     1861 gcctacaagt acccgcccga ggaggtcaac acccgcctgc tcgacatcag agtcaacgtc
     1921 ggccggacgg ggcgggtgac cccgttcggg gtcatgaagc cggtcgtcgt gagcgggtcg
     1981 acggtcgaga tggcgacgct gcacaacgcc gacgaggtcc ggcgcaaggg ggtcctcatc
     2041 ggcgacacgg tggtgctgcg caaggctggg gacgtcatcc ccgagatcgt cggccccgtt
     2101 gtcgagctgc gtgacggcag tgagcgcgag ttcgtcatgc cgacgcactg tccctcgtgc
     2161 ggcaccgccc tggctcccgc gaaggagggc gacaaggaca tccgctgtcc caatacccgg
     2221 gcctgcccgt cccagctgcg ggagcgggtc ttcgggctcg cctcccgtgg agccctcgac
     2281 atcgaggcgc tcgggtggaa g
//
LOCUS       DATAIX010000152         2734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_157862, whole genome shotgun sequence.
ACCESSION   DATAIX010000152 DATAIX010000000
VERSION     DATAIX010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157862"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VHM65_02270"
     CDS             <1..211
                     /locus_tag="VHM65_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704557.1"
                     /translation="TVRIGAVRGSRLAIPGRGRVRGRRRAQRGRRGHDIGLVIEYSVS
                     GKAKRGNCRAGGSTTVDGVTFSHRT"
     gene            222..>2734
                     /locus_tag="VHM65_02275"
     CDS             222..>2734
                     /locus_tag="VHM65_02275"
                     /inference="COORDINATES: protein motif:HMM:NF025181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin-like doman-containing protein"
                     /protein_id="HEX2704558.1"
                     /translation="MLATLVVGLGGTGSWALTHLKQRLLTDQRWGLLGANPGAIGTPG
                     YDTYSGPVVLRALDVDRKNRPRSLEGLVLDPTAEDLAISGPIGRVIEEIQRADESART
                     WPTIQQWLPRSEALTFELGDATRFMVEGAAIRQFGRVAFFLDQLQGAPQLERVVGALS
                     RLSGADRDGRAQGFDLQVFVVASVAGGAGSGLLVDVLTYLQHARRRLAGEVSTRTVGF
                     VVLPGAFSGVVQGDTVEQMQASGRAALRELDRLINAHRTVRVEWLPGRPVKLDAPALD
                     ICYLLDGSRDLSREAPQLEGHVPFENGLPVAIADAIYAHAFPSSGAVFSRDYPNLLPH
                     LIAGTEARYSSFGSYSVNYAWEPLTRSAGMRAAVDMLDHLLAGRESEGAKALGQFLGA
                     GATGSLAVGDVQQPLPAFPTGALSQPQDAGSLLPVSSWLEPRDRLAPAPTQPNLFNHF
                     PGLGKLRTEYDNPDVIKQTQALIEKFWGSRDGSWNGTAEPGFHEAANHNLAQSHREWE
                     RALYVAAVALLNGADRVGGLSAARALLLSLIARVQEFSHQLGALPALDLASYQQAVTD
                     GENAMNDARRWDDMIEQRDYLEARQDLLEQEITAELRTRVSELAGRYLASTREVLAEV
                     DSWRGTLEVVREHAIAGRNAVDAARRAANASPVQRYLPLPGDAVEQELWQDCYGTPGE
                     GLAAGLRQCLETVSWSLTLEEAPNKKIRFATPAAQIRDGGMSLEAALGVTARPFASLR
                     TRSVWEMLERAGTDAGELVAELASRGSPLSVFNPNGQQDVAGNLRQFRDSNYVFTGWL
                     EERADAAESVRGAAPSRAVRARLGEREVHTEPLDTLRNPA"
BASE COUNT          394 a          953 c         1026 g          361 t
ORIGIN      
        1 caccgtgcgg atcggggcgg tccgcggatc ccggctcgcg atccccgggc gggggcgcgt
       61 acggggccgg cggcgggcgc agcgcgggcg ccgcggccac gacatcgggc tggtgatcga
      121 gtactcggtc agtggtaagg cgaagcgcgg caactgccgg gcgggtggca gcacgaccgt
      181 cgacggcgtg accttcagcc atcgaacgtg agaggaccgt catgcttgcg acactggtag
      241 tcgggctggg tggtaccggc agctgggccc tcacgcacct caagcagcgg ctgctgaccg
      301 accagcggtg gggactgctc ggcgccaatc cgggcgcgat cggcacgccc ggttacgaca
      361 cctacagcgg ccccgtcgtg ctgcgcgcac tcgacgtcga ccgcaagaac cggccccggt
      421 cgctggaggg cctggtgctg gacccgaccg cggaggacct ggcgatcagc gggccgatcg
      481 ggcgggtcat cgaggagatt cagcgcgccg acgagtcggc ccggacgtgg ccgacgatcc
      541 agcagtggct gccccgcagc gaagcgctga ccttcgagct cggcgacgcc acccgcttca
      601 tggtggaagg cgcggccatt cgacagttcg gccgggtggc gttctttctc gaccagctgc
      661 agggcgcgcc gcagctcgag cgggtggtcg gcgcgttgtc ccggctcagc ggcgccgacc
      721 gggacgggcg ggcgcagggt ttcgacctgc aggtgttcgt cgtcgcctcg gtggccggcg
      781 gagccggctc gggcttgctg gtcgacgtac tgacctacct ccagcacgcg cggcgccggc
      841 tcgccggcga ggtgtcgacg cgcacggtgg gcttcgtcgt gctgcccggg gccttcagcg
      901 gggtcgtgca gggcgacacg gtcgagcaga tgcaggccag tgggcgagcg gcgctccgcg
      961 agctcgaccg tttgatcaac gcgcaccgca cggtgcgggt cgagtggttg ccgggacgac
     1021 cggtgaaact cgacgcgccg gccctcgaca tctgctatct gctggacggg tcccgggacc
     1081 tctcccggga ggcaccgcag ctggagggac acgtcccctt cgagaacggg ctgcccgtcg
     1141 cgatcgcgga cgcgatctac gcgcacgcgt tcccgtccag cggcgcggtc ttctcgcggg
     1201 actacccgaa ccttcttccg cacctgatcg ccggtaccga ggcccggtac tccagcttcg
     1261 gctcgtacag cgtcaactac gcctgggagc cgctcacccg cagtgcgggg atgcgggccg
     1321 ccgtcgacat gctggaccac ctgctggccg ggcgggagag cgagggggcc aaggcgctcg
     1381 gccagttcct cggcgccggc gccaccgggt ccctcgccgt cggcgacgtt cagcagccgc
     1441 tgccggcgtt cccgaccggc gcgctgtccc agccccagga cgccggctcg ctgcttccgg
     1501 tcagcagctg gctggagccg cgggaccggc tggcacccgc gccgacgcag ccgaacctgt
     1561 tcaaccactt ccccggactg ggcaagctgc gcaccgagta cgacaacccg gacgtcatca
     1621 agcagacgca ggcgctgatc gagaagttct ggggcagccg cgacgggtcg tggaacggca
     1681 ccgccgagcc cggcttccac gaagccgcca accacaacct cgcgcagtcc caccgggagt
     1741 gggagcgcgc cctgtacgtg gcggcggtcg cgctgctcaa cggcgccgac cgggtcgggg
     1801 ggctcagcgc cgccagggcg ctgctgctca gcctgatcgc ccgggtgcag gagttctccc
     1861 accagctcgg cgcgctgcct gcgctggacc tggccagcta ccagcaggcg gtgaccgacg
     1921 gcgagaacgc gatgaacgac gcccgccgct gggacgacat gatcgagcaa cgggactacc
     1981 tcgaggcccg ccaggacctg ctggaacagg agatcacggc ggagctgcgg acccgggtgt
     2041 cggaactcgc cggccgctac ctggcctcca cccgggaggt tctcgccgag gtcgacagct
     2101 ggcgcggcac gctggaagtg gtccgggagc acgcgatcgc cggcaggaac gccgtggacg
     2161 cggcccgcag agcggccaac gcctccccgg tccagcggta cctgccgctg cccggagacg
     2221 cggtcgagca ggaattgtgg caggactgct acgggactcc gggtgagggc ctcgcggccg
     2281 ggctgcggca gtgcctggag accgtgagtt ggtcgctgac gctcgaggag gcgccgaaca
     2341 agaagatccg gttcgccacg ccggcggcgc aaatccggga cggcggcatg tccctcgagg
     2401 cggcgctcgg tgtcacggcc cggccgttcg cgtcgctgcg gacccggtcg gtgtgggaga
     2461 tgctcgagcg cgccgggacg gacgccggcg agctggtggc cgagctcgcg tcccgaggat
     2521 cgccgctgtc ggtgttcaac ccgaacgggc agcaggacgt ggccggcaac ctgcggcagt
     2581 tccgggacag caactacgtc ttcaccggct ggttggagga gcgggccgat gcggccgaga
     2641 gtgtgcgggg agcggcgccg agccgggcgg tgcgggcccg actcggcgaa cgcgaggtgc
     2701 acaccgagcc cctcgacacc ctccgcaacc ccgc
//
LOCUS       DATAIX010000153         3381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_242155, whole genome shotgun sequence.
ACCESSION   DATAIX010000153 DATAIX010000000
VERSION     DATAIX010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242155"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..307)
                     /locus_tag="VHM65_02280"
     CDS             complement(14..307)
                     /locus_tag="VHM65_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002530052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX2704559.1"
                     /translation="MTATPRGYVDTKQTHLAGLRRIEGQIRGLQRMVDDDAYCIDVLT
                     QISAATRALKKVALGLLEDHIAHCVTHAIHAGGQEADAKIKEASAAIARLVRS"
     gene            complement(325..2784)
                     /locus_tag="VHM65_02285"
     CDS             complement(325..2784)
                     /locus_tag="VHM65_02285"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX2704560.1"
                     /translation="MSSHIEDPRNPEQPMATDPVCGMSVDPAGPLVAEHDGRTFHFCS
                     EHCRARFVADPGRYTGAAVPRHQGEPSHSHQQEEPDQHQHQHQHQHAPEPQGSGEPGG
                     EVAQYTCPMHPEIRQPGPGACPICGMALESVMVTTDTGPSKELADMTRRFWVGLALTV
                     PVVLLEMGTHLVGWVRDLIPGTVSTWLQLVLATPVVLWAGWPFFVRGWASVRSRNLNM
                     FTLIAMGTGVAWVYSVVATLAPGIFPAAFRTTSDMSEAGTSGMGTVDVYFEAAAVITV
                     LVLLGQVLELRAREQTSGAIKALLDLTPKTARRIRDDGTDEEIALADVQIGDRLRVRP
                     GERVPVDGTVEEGRSSVDESLVTGESMPVTKTGGDTVIGGTINQTGGLVMRAEKIGRD
                     TMLARIVAMVAEAQRSRAPIQRMADRVAGLFVPAVIGIAVVAFILWALVGPEPRLAHA
                     LIAAVAVLIIACPCALGLATPMSIMVGVGRGAGLGVLIKNAEALERMEKVDTLVVDKT
                     GTLTEGRPSVTAIVPAEGFADDEVLRLAAGVERASEHPLAQAVVEAAQGRDLAIPDVD
                     DFDSPVGRGVQGRVEGHAVVLGSSAFLTSHQIDTAALDARAAQLRGDGATVIYIGVDA
                     QAAGLFAIADPVKATTPAALAALRTDGIEVVMLTGDNRVTAEAVGRRLGIDRVEAEVL
                     PDHKSDVVKALRDQGRVVAMAGDGVNDAPALAAADVGLAMGSGTDVAMESAGVTLLHG
                     DLTGIVRARQLSVKTMSNIRQNLLFAFVYNVAGIPIAAGVLYPMFGLLLSPIIAAAAM
                     ALSSVSVIGNALRLRAQDLET"
     gene            complement(2861..3157)
                     /locus_tag="VHM65_02290"
     CDS             complement(2861..3157)
                     /locus_tag="VHM65_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704561.1"
                     /translation="MLPHVMHHIGLLAGAALLTGVVGSAVLYALGILLSIPMLRRLHT
                     RFGTRWAPAVGVAVFTGLFALSAFVIGPAISVTASTPPAPTAPTVAPDVHTGHH"
BASE COUNT          484 a         1132 c         1252 g          513 t
ORIGIN      
        1 ccattggaag gtgtcaggag cggacgagtc gtgcaatggc ggcggaggcc tccttgatct
       61 tggcatcggc ctcctgaccg ccggcgtgga tggcgtgggt gacgcagtgg gctatgtggt
      121 cctcgagcaa gccgagggcg actttcttga gggctcgggt ggcggccgag atctgggtga
      181 gcacgtcgat gcagtaggca tcgtcgtcga ccatgcgctg caggccccgt atctggccct
      241 cgatgcggcg cagccccgcc agatgagtct gcttggtgtc gacgtaccca cgtggtgtcg
      301 cggtcatgac cgggtccctt cggctcaggt ctcgaggtct tgggcacgca ggcgcagggc
      361 gttgccgatg acgctgaccg aggacagggc catggcggcg gcggcgatga tcggcgagag
      421 cagcagcccg aacatcgggt agagcacccc ggcagcgatc gggatgccgg ccacgttgta
      481 gacgaaggcg aagagcaggt tctggcggat gttgctcatc gtcttgaccg agagctggcg
      541 ggcccggacg atgccggtga ggtcgccatg cagcagggtg acgccggcgc tctccatggc
      601 cacgtccgtg cccgatccca tggcgagccc gacgtccgcc gcggcgaggg cgggcgcgtc
      661 gttgacgccg tcgccggcca tcgccacgac tcgtccctgg tcccgcagtg ccttgacgac
      721 gtcgctcttg tgatcgggca ggacttcggc ctcgacccgg tcgatcccca ggcgccggcc
      781 gaccgcctcg gccgtcacgc ggttgtcccc ggtgagcatg acgacctcga tgccgtcggt
      841 gcgcagcgcg gccaacgctg ctggggtggt ggccttgacg gggtcggcga tggcgaagag
      901 accggccgcc tgggcgtcga cgccgatgta gatgacggtg gctccgtcgc cgcgcagctg
      961 ggcggcccgg gcgtcgaggg cggcggtgtc gatctggtga gaggtcagga aggccgagct
     1021 gcccaggacc acggcgtggc cctcgacccg cccctgcacg cctcggccca cgggtgagtc
     1081 gaagtcgtcg acgtccggga tggcgaggtc gcggccctgg gcggcctcga cgacggcttg
     1141 cgccaagggg tgctcggagg cacgctccac accggcggcc agacgcagga cctcgtcgtc
     1201 ggcgaaccct tcggcgggca cgatggcggt gaccgaggga cgaccctcgg tgagggtgcc
     1261 ggtcttgtcc acgacgaggg tgtcgacctt ctccatccgc tcgagcgcct cggcgttctt
     1321 gatgagcacg ccgagcccgg cgccgcgccc gacgccgacc atgatcgaca tcggggtcgc
     1381 caggccgagg gcgcaggggc aggcgatgat cagcacggcg actgcggcga tgagggcgtg
     1441 ggcgagccgc ggttcggggc cgacgagggc ccagaggatg aacgcgacga cggcgatgcc
     1501 gatgacggcg gggacgaaca gcccggccac ccggtcggcc atccgctgga tcggggctcg
     1561 ggagcgttgg gcctcggcga ccatggcgac gatccgcgcg agcatggtgt cgcggccgat
     1621 cttctccgcg cgcatcacca gaccgccggt ctggttgatg gtgccgccga tgaccgtgtc
     1681 tccgccggtc ttggtgaccg gcatcgactc cccggtgacc agggactcgt ccaccgagga
     1741 ccggccctcc tcgacggtgc cgtcgacggg cacccgctcg ccgggccgga cccgcaaccg
     1801 gtccccgatc tgcacgtcgg ccagggcgat ctcctcatcg gtcccgtcgt cgcggatgcg
     1861 ccgagcggtc ttcggggtca ggtcgagcag cgccttgatt gccccggagg tctgctcccg
     1921 cgcccgcagc tccagcacct gccccagcag gaccagcacg gtgatgacgg cggcggcctc
     1981 gaagtagacg tcgacggtgc ccatgccgct cgtgccggcc tcgctcatgt cgctcgtggt
     2041 gcggaaggcg gcggggaaga tgccgggcgc gagcgtcgcg acgacgctgt acacccacgc
     2101 cacaccggtg cccatcgcga tgagggtgaa catgttgagg ttccggctcc ggaccgacgc
     2161 ccagccccga acgaagaacg gccagccggc ccacagcacc accggggtgg cgagcaccag
     2221 ctgcagccag gtcgagaccg tgcccgggat caggtcacgg acccagccga ccaggtgggt
     2281 tcccatctcc agcaggacca ccggcacggt cagggccagc ccgacccaga agcggcgggt
     2341 catgtccgcc agctccttgc tgggcccggt gtcggtggtc accatcaccg actcgagcgc
     2401 catcccgcag attgggcacg cacccgggcc gggctggcgg atctcggggt gcatcgggca
     2461 ggtgtactgt gcgacctccc ccccgggctc gccactgccc tgcggctccg gtgcgtgctg
     2521 gtgctggtgc tggtgctggt gctggtcggg ctcctcctgc tggtgcgagt ggctgggctc
     2581 tccctggtgc cggggaacgg ccgcgccggt atacctgccc gggtcggcca cgaaccgggc
     2641 ccggcagtgc tcggagcaga agtggaaggt gcgcccatca tgctcggcga cgagcgggcc
     2701 ggccgggtcg acgctcatgc cgcacaccgg gtcggtggcc atgggctgct cggggtttcg
     2761 cggatcctcg atatggctgc tcatgccatc cagaataccc ctacccccta agggtataca
     2821 agagggattg gtcgtggatg gtcgatcccg gcgaccacgg tcagtggtgg cctgtgtgca
     2881 cgtcaggcgc cacggtcggg gcggtggggg ctggtggcgt gctggcggtc acgctgatcg
     2941 cgggtccgat gacgaacgcc gagagcgcaa acagtccggt gaagacggcg actcccacgg
     3001 ctggagccca ccgggtgccg aagcgggtgt ggagtcggcg cagcatcggg atgctcagca
     3061 gaatcccgag cgcgtacagc acggcactgc cgacgacgcc ggtcagcagg gcagcgccgg
     3121 ccagcagccc gatgtggtgc atcacgtgtg gcagcacacc cagggcagca ccgagagctg
     3181 ccctgacgcc ggcccagagc ccccgtcccg ttcccgggtc cgcgaccgaa gcggtgtcga
     3241 cgggctcggc ggcaggtccc ctggtttgct gggtcacgac tgggtctggc tctctggtct
     3301 ggggtggccc aactatccgc gagttctgat cgtggccgcc gtttcccgcg gacgccctgt
     3361 acatccggtc aagatccgat g
//
LOCUS       DATAIX010000154         1940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_8383, whole genome shotgun sequence.
ACCESSION   DATAIX010000154 DATAIX010000000
VERSION     DATAIX010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8383"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="VHM65_02295"
     CDS             <1..995
                     /locus_tag="VHM65_02295"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX2704562.1"
                     /translation="ELRNKGITCIIISHKLNEIAQIADSITIIRDGQSIETLDVKGDA
                     VDEDRIIRGMVGRPLESRFPDYTPSIGEVFFEVRDWTVESPIVPGRMVCKGSSFTVSR
                     GEIVGFAGLMGAGRTELMRSLFGHSYGGFREGTMILDGKEVQLRSVPAAIDAGIGYVT
                     EDRKMLGLNLLDDIKATTVSAKLKKITRHFGVDRRAEFQAAEGYRKSLRTKTPTVNEG
                     VSTLSGGNQQKVVLAKWLFTDPELLILDEPTRGIDVGAKFEIYGIIHQLAAEGKGIIV
                     VSSELPELLGLSDRIYTICEGAITGCLTKAEATQENLMRLMTTTSDHTPYRQSA"
     gene            1010..>1940
                     /locus_tag="VHM65_02300"
     CDS             1010..>1940
                     /locus_tag="VHM65_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704563.1"
                     /translation="MKKLKDIFGGDTRQFGMILALVALIVLFQITTGGTTLTPDNVIN
                     LFNGNSYILVLAVGMVLVIIAGHIDLSVGSIAAAVGIIVAIAMRDWGLAWPVGILLGL
                     IVGALIGAWQGFWVAYVGIPAFIVTLAGMLIFRGVNQFVGKSNSVPVSTDFQVIGAGY
                     LPEVGPDTGYNNLTVLVGLAGAAAIAYGQWRGRRGAIKVGSPVAPFWAFVTKIALVCG
                     AVLAATLLFASGRPGTSFPISGLILGILVLIYGFISTRTVVGRHIYAVGGNRHAAELS
                     GVRSKQVNFLVMMNMAVLAALAGMMFVARSTSSG"
BASE COUNT          320 a          674 c          611 g          335 t
ORIGIN      
        1 acgagctgcg caacaagggc attacgtgca tcatcatcag ccacaagctc aacgagatcg
       61 cccagatcgc cgactcgatc acgatcatcc gggacggtca gtcgatcgag accctcgacg
      121 tcaagggcga cgccgtcgac gaggaccgga tcatccgcgg catggtcggc cgaccgctgg
      181 agtcgcggtt ccccgactac acgccctcca tcggggaggt cttcttcgag gtgcgggact
      241 ggacggtcga gagcccgatc gtccccggcc ggatggtgtg caagggctcg tccttcaccg
      301 tgagccgcgg tgagatcgtc gggttcgccg gtctcatggg tgcgggccgc accgagctga
      361 tgcgttcgct cttcggtcac tcctacggcg ggttccgcga gggcacgatg atcctcgacg
      421 gcaaggaagt ccagctgcgc agcgtcccgg ccgcgatcga cgccggcatc ggctacgtca
      481 ccgaggaccg caagatgctc gggctgaacc tgctcgacga catcaaggcg acgaccgtgt
      541 cggccaagct gaagaagatc acgagacact tcggcgtcga ccgccgggcc gagtttcagg
      601 ccgccgaggg ctaccgcaag tcgctgcgga ccaagacgcc gaccgtcaac gagggcgtct
      661 ccaccctctc cggcggcaac cagcagaagg tcgtcctggc caagtggctg ttcaccgacc
      721 ccgagctgct catcctcgac gagccgaccc ggggcatcga cgtcggcgcc aagttcgaga
      781 tctacggcat catccaccag ctcgccgccg agggtaaggg catcatcgtc gtctcctccg
      841 agctgcccga gctgctcgga ctgtccgacc ggatctacac gatctgcgag ggcgcgatca
      901 ccgggtgcct caccaaggcc gaggccaccc aggagaacct catgaggctg atgacgacca
      961 cctccgacca caccccctac cgccagtcgg cctgacccga aggacgtcga tgaagaagct
     1021 caaggacatc ttcggcgggg acacccgcca gttcggcatg atcctcgcgc tcgtcgcgct
     1081 gatcgtgctg ttccagatca cgaccggcgg gacgacgctg acaccggaca acgtcatcaa
     1141 cctgttcaac ggcaactcct acatcctcgt cctggccgtc ggcatggtgc tcgtcatcat
     1201 cgccggtcat atcgacctct cggtggggtc gatcgcggcg gctgtcggca tcatcgtggc
     1261 gatcgcgatg cgcgactggg ggctggcctg gccggtcggg atcctgctgg gcctcatcgt
     1321 cggagcactc atcggggcct ggcagggctt ctgggtcgcg tatgtcggaa tcccggcctt
     1381 catcgtcacc ctggcgggga tgctcatctt ccggggggtc aaccagttcg tcggcaagtc
     1441 gaactcggtc ccggtctcga ccgacttcca ggtcatcggc gccggctacc tacccgaagt
     1501 cgggcctgac acggggtaca acaacctcac cgtgctcgtc ggtctggccg gtgccgcagc
     1561 catcgcctac ggccagtgga ggggtcgccg cggcgccatc aaggtgggct caccggtggc
     1621 cccgttctgg gccttcgtca ccaagatcgc cctggtctgc ggggccgtcc tcgccgcgac
     1681 gctgctgttc gcctccggcc ggccaggcac gtcgttcccg atctcgggcc tgatcctcgg
     1741 cattctcgtg ctcatctacg gtttcatctc gacgcgcacc gtcgtcggtc ggcacatcta
     1801 cgccgtgggc ggcaaccggc acgcggccga gctctcgggt gtcaggagca agcaggtcaa
     1861 cttcctcgtc atgatgaaca tggccgtcct ggcggcgctg gcaggcatga tgttcgtcgc
     1921 ccgctccacc tcctcgggcc
//
LOCUS       DATAIX010000155         2057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_212319, whole genome shotgun sequence.
ACCESSION   DATAIX010000155 DATAIX010000000
VERSION     DATAIX010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212319"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..248)
                     /locus_tag="VHM65_02305"
     CDS             complement(33..248)
                     /locus_tag="VHM65_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704564.1"
                     /translation="MAKYDTNVTTLGELLEDPAVVEIIDRHAPGLATNPMIGMAKGMP
                     ASQALSMACGMIGKDSADAIRADVEAL"
     gene            complement(364..>2057)
                     /locus_tag="VHM65_02310"
     CDS             complement(364..>2057)
                     /locus_tag="VHM65_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX2704565.1"
                     /translation="AAQGDLDAWFACAQALRRYGGAWVSDSERAELREGVLRLVLGSR
                     SQARSRLARLLESASLDAVVGDELAQNLESGDVDLADVFALTEYASARTLLDGVAGLG
                     TAAPDDPECARLERAITAYTAPDDDSPIARQMRVVSELSLGSTQEERTERSGLVAELE
                     QRYAALGCGYEGMAEPVSLAAAQELLGADELLVRYVLPYHALHPAYLPSVLVVTRTEA
                     RLVRLPVPDSGIGLIGRLSIDGRAPVDASPLGNEVVLARSAMDGGLDGDASPHLHRLY
                     AMLVEPVLSTGLAANRNRWVIAPQRSLHLVPWMALQDASGRWLLDDAAVTIAPSASVW
                     AALRRRPPRPAGALAAVGDPLVGYAGLPQLPGARAEVDHLAGLFQASGRPVRSLLGAA
                     ATYDGLVGAVQLAGLVHVATHGSFPEDGALFDHRILLSQDLGRPGPVTTDALRRLDLG
                     AAWCVTLSVCNGGVFRVGPGDEPYGLVPAVLEAGSPTVVAAQWAVDDDAGRELMRRVA
                     DRIVEAGPAEALRGAVLEARRTGARPARDYTAFLAIGTGLGPAGDTSNVMEGRDR"
BASE COUNT          279 a          762 c          757 g          259 t
ORIGIN      
        1 ggggtgcagg gccaggggtg caggggtgca ggctagagcg cctcgacgtc ggcgcggatc
       61 gcgtcggcgc tgtccttgcc gatcatcccg caggccatgc tcagtgcctg gctggccggc
      121 atgcccttgg ccatgccgat catggggttg gtcgcgaggc caggagcgtg cctgtcgatg
      181 atctcgacga cagccgggtc ctccagcagc tcgccgagcg tcgtgacgtt ggtgtcgtac
      241 ttggccatgg tcttcctccc gttgagtgcg tgcgctggcc gcccggacgg cgcacccgga
      301 ggccagcccc gaccctacct cgcagcgacc ccccgacgca gaacgaccgg gcgggccggt
      361 gacctaccgg tccctgccct ccatgacgtt cgacgtgtca ccagcaggtc ccaggccggt
      421 cccgatggcg aggaacgccg tgtagtcgcg ggccggccgg gccccggtcc gacgggcctc
      481 gagcacagca ccccgcagcg cctcggccgg gccggcctcg acgatccggt cggcgacgcg
      541 gcgcatgagc tcgcgccccg cgtcgtcgtc gacggcccac tgggccgcca cgacggtcgg
      601 gctgccggct tccagcaccg cgggcaccag gccgtaaggc tcgtccccgg gcccgacccg
      661 gaatactccg ccgttgcaca cgctcagcgt cacgcaccac gcggcaccca ggtcgagccg
      721 gcgcagcgcg tccgtcgtca ccgggccggg acggccgagg tcctgggaga gcaggatccg
      781 gtggtcgaag agcgccccgt cctcgggaaa ggacccgtgg gtggcgacgt gcacgagccc
      841 cgcgagctgg accgcgccga ccagcccgtc gtatgtcgcg gcggcgccga gcagggaccg
      901 caccgggcgt ccactcgcct ggaacaggcc agccaggtgg tcgacctcgg cccgtgcccc
      961 cggcagctgc gggagaccgg catacccgac gagggggtcc ccgacggcgg ccagcgctcc
     1021 ggcgggccgg ggtggacgac gacgcagggc tgcccacaca gacgcactcg gcgcgatggt
     1081 cacggcggcg tcgtcgagca gccaccggcc agaggcatcc tgcagcgcca tccaggggac
     1141 gagatgcagc gagcgctggg gcgcgatgac ccaccggttc cggttcgcgg ccagcccggt
     1201 gctcagcacg ggttcgacga gcatggcgta gagccggtgc aggtgcgggc tcgcgtcccc
     1261 gtcgagaccg ccgtccatgg cgctccgggc caggacgacc tcgttgccca gggggctcgc
     1321 gtcgaccggg gctcgcccgt cgatcgagag gcgcccgatg aggccgatgc cggagtccgg
     1381 caccggcagg cggacgagcc gcgcctcggt ccgggtcacg acgagcacgc tcggcaggta
     1441 ggccgggtgc agcgcgtggt agggcaggac ataccggacg agcagctcgt ccgccccgag
     1501 gagctcctgg gccgcggcga gcgagaccgg ctcggccatc ccctcgtagc cgcagccgag
     1561 cgcggcatac cgctgctcca gctcggcgac gagcccgctc cgttcggtgc gctcctcctg
     1621 ggtactgccg agtgacagct cgctgacgac gcgcatctgc cgggcgatcg gcgagtcgtc
     1681 gtcgggtgcg gtgtatgccg tgatcgcgcg ttcgagccga gcgcactccg ggtcgtccgg
     1741 tgccgcggtg cccagacccg cgaccccgtc gagcagggtc cgcgccgacg cgtactcggt
     1801 gagcgcgaag acgtcggcga ggtccacgtc gccgctctcc aggttctgcg cgagctcgtc
     1861 tccgaccacg gcgtcgaggc tggccgactc gagcagccgc gccagccggc tgcgggcctg
     1921 ggagcgcgat cccagcacga gccgcaggac tccttcgcgc agctcggcgc gctcggagtc
     1981 gctcacccac gcgccgccat agcggcgcag cgcctgggca caagcgaacc atgcgtcgag
     2041 gtcgccctgc gccgcta
//
LOCUS       DATAIX010000156         2808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_68589, whole genome shotgun sequence.
ACCESSION   DATAIX010000156 DATAIX010000000
VERSION     DATAIX010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68589"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="VHM65_02315"
     CDS             complement(<1..1246)
                     /locus_tag="VHM65_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX2704566.1"
                     /translation="MKKAALLTPLLAVLFVPLFAAAFLASAQTPALAEQVRATQCSSA
                     AIPATGAWRPPFQQAYALTSGFGQRFDPVYQRWQRHAGQDLASLPGPGPVVAAAAGKV
                     TRAGPYGGYGNAVVLDNGAGTETTYGHLAGIDPKITVEATVWMGQKLGVEGSTGASTG
                     SHLHFELHQDGTPIDPVPFMLAHGAPLTGHAVAPSKPPTTDPSSGTSSSVEGGIGFPL
                     PEPGTPRQDSLHSPPTPIPAGVKALYVAAADRYKIPWTLLAGIGMEETGHGRNLGTSS
                     AGAQGLMQLTPATWAQYGVDGDGDGRAVISDGADSVMSAASYLTRLGVSAGGQAGVSA
                     AIFGYNHGDWYVNDVLFYANAYGGGTILGDPSDCGPGGNGNPTLPPLASERIATVLTW
                     AKAQIGEPYVFGANGPNAWDCSS"
     gene            complement(1265..>2808)
                     /locus_tag="VHM65_02320"
     CDS             complement(1265..>2808)
                     /locus_tag="VHM65_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704567.1"
                     /translation="AVRRPVRRTESGRGRDVSRLLSDFGHDLPSVPAAGPRAKEARLF
                     QHAPRGGPRRPDGGWAPAKAPVMSYRMTSDQAPVFWPFIATPGLPPTGAQMGIDMLSG
                     GSFYTDPLGWVLRDEIPVTNPNIFSFGKPGTGKSATTKAFCLRMMDFGYRTFILGDPK
                     DEYEPLCRFFGVEPFVIGPGMSTRINPLAFGPLVMGWDRLTPAAATSRVAVIFGRWLT
                     LVRGLVGSQRIGTHHVPFGPTEDRVVKAALQDLTGYSRGATALHESTIPELWHLLDNP
                     TDELVEQCRYACARHFLDETRLLRDALGQLVNGALAGLFDGHTTIEVDWAAPIQSLSL
                     SRLEPLGDDAVGIALTCLNSWGRGMRERAEPGDLRIVVRDESWKQLRLGPEAVKSFDA
                     DLRLSRGCAGMGGDIQLAVAHKPSDLLSAGDAGSQAATIAKDLLHLADIKILHGQKAQ
                     VAAELDTMLGLGPMARDLITGWAMQSKGRALWCVGEQTYKVQTVLHPIEKNLTFTNQA
                     IETAG"
BASE COUNT          369 a          942 c         1060 g          437 t
ORIGIN      
        1 agctggagca gtcccacgcg ttggggccgt tggccccgaa gacgtagggc tcgccgatct
       61 gggctttggc ccaggtcagc accgtggcga tgcgttcgct ggccagtggg ggcagggttg
      121 ggttgccgtt gccgccaggc ccgcagtcgc tggggtcccc caggatggtg ccgccgccgt
      181 aggcgttggc gtagaacagg acgtcgttga cgtaccagtc gccgtggttg tacccgaaga
      241 tcgcggcgct gaccccggcc tgtcccccgg cgctgacgcc gagcctggtc aggtagctgg
      301 ctgcgctcat gacggagtcg gcgccgtcgc tgatcactgc gcgcccgtcg ccgtcgccgt
      361 cgacgccata ctgcgcccag gtggcgggcg tgagctgcat cagcccctgg gcgccggcgc
      421 tggaggtgcc caagttccgg ccgtggcctg tctcttccat cccgatcccg gccagcaggg
      481 tccacgggat cttgtaccgg tcggcggccg cgacgtacag ggccttgacg ccggccggga
      541 tcggcgtcgg cgggctgtgc agcgagtcct ggcgtggggt gcccggctcg ggcaggggaa
      601 acccgatgcc tccctcgacc gagctcgatg ttccggacga ggggtcagtg gtcggtggct
      661 tggagggtgc gacggcgtgc ccggtcaggg gcgccccgtg agccagcatg aacggcaccg
      721 ggtcgatcgg cgtcccgtcc tggtgcagct cgaagtgcag gtgactgcct gtcgacgccc
      781 cggtggagcc ttcgacgccg agcttctggc ccatccagac ggtggcctcg acggtgatct
      841 tcgggtcgat gccggccagg tgcccgtacg tggtttcggt gccggcgccg ttgtccagga
      901 cgacggcgtt gccgtagccg ccgtagggcc cggcgcgggt gaccttcccg gcggccgcgg
      961 cgaccaccgg gccgggcccg ggcagggagg ccaggtcttg cccggcgtgg cgctgccagc
     1021 gctggtagac cgggtcgaag cgttgcccga agccgctggt gagggcgtac gcctgctgga
     1081 acggcggccg ccatgccccg gtcgcgggta tggcggcact ggagcactgg gtggccctga
     1141 cctgttcggc gagggcgggt gtctgcgccg aggccaggaa cgcggccgcg aacagcggca
     1201 cgaacagcac cgccagcagc ggggtcagca gtgctgcctt cttcatagcg cgctcccccg
     1261 tcgcctagcc ggctgtctcg atggcctggt ttgtgaaggt gaggttcttc tcgatggggt
     1321 gcagcacggt ctggaccttg taggtctgct ctccgacgca ccacagggcc cggcccttgc
     1381 tttgcatcgc ccagccggtg atcaggtccc gggccatcgg gcccagcccg agcatggtgt
     1441 ccagctcggc cgcgacctgg gccttctgcc cgtgcaggat cttgatgtcg gccaggtgca
     1501 gcaggtcctt ggcgatcgtg gcggcctggg agccggcgtc gccggccgag agcaggtcgg
     1561 agggtttatg ggcgacggcg agctggatgt caccgcccat cccggcgcag ccgcgggaca
     1621 ggcgcaggtc tgcgtcgaag ctcttgacgg cctccgggcc gaggcgcagc tgcttccagg
     1681 actcgtcgcg cacgacgatg cgcaggtccc cgggctcggc cctctcccgc atcccccggc
     1741 cccaggagtt caggcaggtc agcgcgatcc cgaccgcgtc gtcaccgagg ggttcgagcc
     1801 gggacaggct cagggactgg atcggggcgg cccagtccac ctcgatggtg gtgtggccgt
     1861 cgaacaaccc ggccagggcg ccgttgacca gctgcccgag ggcgtcacgc agcagcctgg
     1921 tctcgtccag gaagtgcctg gcgcaggcgt accggcactg ctcgaccagc tcgtcggtgg
     1981 ggttgtccag cagatgccac agctcgggga tggtcgactc gtgcagcgcg gtggcgccgc
     2041 ggctgtaacc ggtgaggtcc tgcagggcgg ccttgacgac gcggtcctcg gtcggcccga
     2101 acggcacgtg gtgggtgccg atccgctggc tgccgaccag gccgcgcacc agggtgagcc
     2161 agcgcccgaa gatgaccgcg acccggctgg tcgccgccgc cggggtgagc cggtcccagc
     2221 ccatgaccag cgggccgaac gcgagcgggt tgatgcgggt gctcatgccc gggccgatga
     2281 cgaacggctc gaccccgaag aaccggcaca gcggctcgta ctcgtctttg gggtcgccga
     2341 ggatgaaggt ccggtacccg aagtccatca tccgcaggca gaacgctttc gtcgtcgcgg
     2401 acttgccggt gccgggcttg ccgaagctga agatgttcgg gttggtgacg gggatctcat
     2461 cgcgcaggac ccagccgagg gggtcggtgt agaacgagcc tccggagagc atgtcgatgc
     2521 ccatctgcgc gccggtcggc ggcagcccgg gtgtggcgat gaacggccag aacaccggcg
     2581 cctggtcgga ggtcatccga tacgacatga ccggggcctt cgccggtgcc cacccgccgt
     2641 ctgggcggcg aggcccgccg cgtggcgcgt gctggaacaa ccgggcctcc ttggcccgcg
     2701 gcccggcggc cggcaccgac ggaaggtcgt ggccgaagtc ggacagcagc cgggacacgt
     2761 cacgtccgcg gccggactcg gtgcgcctca cagggcgcct caccgcgt
//
LOCUS       DATAIX010000157         4050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_158039, whole genome shotgun sequence.
ACCESSION   DATAIX010000157 DATAIX010000000
VERSION     DATAIX010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158039"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..451)
                     /locus_tag="VHM65_02325"
     CDS             complement(131..451)
                     /locus_tag="VHM65_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX2704568.1"
                     /translation="MPERLPIRMLQDRVLVSVEHEGGERRSGGGILIPATASVGKRLS
                     WAAVVATGSSVRQVEIGDRVLFDPEDRHEVELGSKEYILLRERDIHAVAATRVQNGQT
                     GLYL"
     gene            complement(480..758)
                     /locus_tag="VHM65_02330"
     CDS             complement(480..758)
                     /locus_tag="VHM65_02330"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX2704569.1"
                     /translation="MSESNGASPTRSVAEIEAEMATTRARLAGTIDELSVRAQPKEIA
                     RRQAQSARAKLSAALTTPEGDLRVERLAIIAAAVVVLIALRHVARRRR"
     gene            complement(814..1281)
                     /gene="bcp"
                     /locus_tag="VHM65_02335"
     CDS             complement(814..1281)
                     /gene="bcp"
                     /locus_tag="VHM65_02335"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEX2704570.1"
                     /translation="MAERLTPGDVAPDFDLTNDAGEQVRLAGLRGRKVIVYVYPAAMT
                     PGCTTQACDFTESLGSLRSHGYEVLGISPDQPGKLATFRKRDALTITLLSDPDKTVMS
                     AYGAYGQKQMYGRTVQGVIRSTFVIDEQGRIQHAFYNVKATGHVAKLRRDLGL"
     gene            1413..2105
                     /locus_tag="VHM65_02340"
     CDS             1413..2105
                     /locus_tag="VHM65_02340"
                     /inference="COORDINATES: protein motif:HMM:NF014007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEX2704571.1"
                     /translation="MHVPDGFLNAPTSLATGAVAVAGVALALRGSRRELDDRTAPLAG
                     LVATFIFATQMLNFPVAAGTSGHLLGGALAAVLVGPWTGTLCLAVVLGVQALLFADGG
                     LTALGTNITLMGLVGVWAGYGGFRLALALLPRRVRLVPAAAAIGALVSVPTAALGFVG
                     LFALGGQVPVPLATLAAAMGSVHVLIGIGEAVITFLVVASITAVRPDLVHGARFVQAK
                     PMLSDEREQAAV"
     gene            2102..2455
                     /locus_tag="VHM65_02345"
     CDS             2102..2455
                     /locus_tag="VHM65_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HEX2704572.1"
                     /translation="MTRLPTRALVGIGLALALFLAGFVSYYASSEPDGLERVATDKGF
                     IGSAAGHGAAGSPLAGYGVAGVDNPRLSAGLAGVAGTLVVLVTAGGLTWALRRTRQRA
                     GQDTGDGARPPTSSG"
     gene            2466..3233
                     /gene="cbiQ"
                     /locus_tag="VHM65_02350"
     CDS             2466..3233
                     /gene="cbiQ"
                     /locus_tag="VHM65_02350"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HEX2704573.1"
                     /translation="MGAGHGHTLHYHGHSLLHRMPAHLKVVAVLGFVLAVVATPPEQY
                     WAFAVHAALVGAAVIASRVPVRYLAARLVIEVPFLGFAALMPFVSTGPRVDVLGLSLS
                     SAGLLGGWALVAKGTLGVLASLVLATTTEPRDLLRGLERLRLPRQLTQIMGFMVRYVD
                     VVGDEMRRMRVARESRGFSARSPRHWGVLAQSAGALFIRAYERGERVHLAMLSRGYSG
                     TLPAGHDVATTRADAARAAILPGCALLVALLAVQAMR"
     gene            3241..3996
                     /locus_tag="VHM65_02355"
     CDS             3241..3996
                     /locus_tag="VHM65_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2704574.1"
                     /translation="MSTPVLDLRGLAYAYPDGHRALNGVDLHVHRGERVALLGPNGAG
                     KTTLVLHLNGILTPGAGSVAVSGLPVVKANLAEVRRRVGIVFQDPDDQLFMPTVRDDV
                     AFGPANAGLTGPALDRRVAAALDAVGMRELADRPPHHLSFGQRRRVSVATVLAMEPRI
                     LVLDEPSSNLDPASRRELADIVRSLDVTVLMVTHDLPYALELCERSVILSGGTIVADG
                     PTRDVLADTELMTAHRLELPFGFDPNATVGSRG"
BASE COUNT          490 a         1526 c         1398 g          636 t
ORIGIN      
        1 gctcaatggc agagcaagtg actcttaatc actgggttcg gggttcgaat ccctggcggc
       61 gcaccacaca aagaccagcg ggcccagggt catccggggc ccgctggctc gttctgcgcc
      121 gccggccggg tcacaggtac agcccggtct ggccgttctg gacccgggtc gctgcgacgg
      181 cgtggatgtc tcgctcgcgc agcaggatgt actccttcga gccgagctcg acctcgtggc
      241 ggtcctcggg gtcgaagagc acccggtcgc cgatctcgac ctggcgcacc gaggagccgg
      301 ttgccacgac tgctgcccag gacaggcgct tgccgaccga cgcggtcgcg gggatgagga
      361 tgccgccgcc ggatcggcgc tcgccgccct cgtgctcgac cgagacgaga acgcggtcct
      421 ggagcatgcg gatgggcagg cgctcaggca ccgaccgcgt ggccgaggac gagcccacgt
      481 cagcgacggc ggcgcgcgac gtgacgcagc gcaatgagca cgaccaccgc ggcggcgatg
      541 atcgcgaggc gctcgacccg caggtcgccc tcgggcgtgg tcagggctgc ggacagcttg
      601 gcccgggccg actgagcctg acggcgagcg atctccttgg gctgggcgcg caccgagagc
      661 tcgtcgatcg tgcccgccag acgtgctcgc gtcgttgcca tctcggcctc gatctcggcg
      721 acggaccgag tcggcgacgc gccgttggac tcgctcatgc tctctcctcg gctggtcagt
      781 cgacgaaccc gacgggcccc actctagcga ccctcacagg cccaggtcgc ggcgcagctt
      841 ggcgacgtga ccggtggcct tgacgttgta gaacgcgtgc tggatccggc cctgctcgtc
      901 gatgacgaac gtcgaccgga tgacgccctg gacggtgcgc ccgtacatct gcttctggcc
      961 gtacgcgcca tacgcgctca tgacggtctt gtccgggtcg gacagcaagg tgatggtgag
     1021 ggcgtctcgc ttgcggaacg tggcgagctt gcccggctgg tccggagaga taccgaggac
     1081 ctcgtagccg tggctgcgca gcgagccgag cgactcggtg aagtcgcacg cctgcgtggt
     1141 gcagcccggg gtcatcgcgg ccgggtagac gtagacgatg accttgcgcc cccgaaggcc
     1201 ggcgaggcgg acctgctccc cggcgtcgtt ggtgaggtcg aagtcgggtg ccacgtcccc
     1261 gggggtgagt cgctcggcca tccgggctcc ttcgtcgtcg gtgcgcccgg cccgacggac
     1321 cggtcagggc gggcgggtgt tcttattgca aagtgattgc aataaggtgg tggggtcggc
     1381 cggaccagcc ggctccgcca ccaggagcaa ccatgcacgt cccagacgga tttctcaacg
     1441 cacccacgtc tctggcgacc ggcgccgtcg ccgtcgccgg ggtcgccctc gcccttcgcg
     1501 ggtcgcgccg cgagctcgac gaccgcacgg cccccctggc cggcctggtc gccaccttca
     1561 tcttcgccac ccagatgctg aacttcccgg tcgcggccgg cacctcgggc catctgctcg
     1621 gcggcgccct cgccgccgtc ctcgtcggcc cttggacggg cacgctgtgc ctcgcggtcg
     1681 tcctcggtgt ccaggccctg ctcttcgccg acggtgggct caccgcgctc ggcacgaaca
     1741 tcaccctcat ggggctggtc ggtgtctggg ccgggtatgg cgggttccgc ctcgcgctcg
     1801 ctctccttcc ccgccgggtc cggctcgtcc cggccgcggc tgccatcggg gcgctcgtct
     1861 cggtccccac ggcggcactg ggcttcgtcg gcctgttcgc gctcggcggg caggtgcccg
     1921 tcccgctcgc gaccctggcc gcggcgatgg gctcggtcca cgtcctcatc ggtatcggtg
     1981 aggccgtcat caccttcctc gtcgtcgcga gcatcaccgc ggtccgtccc gacctggtcc
     2041 acggcgcccg cttcgtgcag gccaagccca tgctctccga cgagagagag caggccgcgg
     2101 tatgacgcgc ctgcccaccc gggccctcgt cggcatcggc ctggccctcg cgctcttcct
     2161 cgccgggttc gtgagctact acgcgtcctc cgagcccgac gggctcgaac gggtggccac
     2221 cgacaagggc ttcatcggca gcgcggccgg gcacggcgcg gccggctcgc ccctggccgg
     2281 atacggcgtc gccggggtcg acaacccgcg gctctccgcc gggctggccg gagtcgcggg
     2341 gacgctcgtc gtgctcgtca ccgccggcgg tctcacctgg gccctgcgtc gcacgcgtca
     2401 gcgcgcgggc caggacaccg gcgacggcgc ccgcccgccc acgtcgtcgg gctaggacgg
     2461 cgccggtggg ggctggacac gggcacacgc tgcactacca cgggcactcg ctgctgcacc
     2521 ggatgcccgc gcacctcaag gtcgtcgccg tgctcggctt cgtcctcgcg gtcgtcgcga
     2581 ctccacccga gcagtactgg gccttcgccg tccacgccgc cctcgtcggt gccgccgtca
     2641 tcgcctcccg cgtcccggtg cgctatctcg ctgctcgcct ggtcatcgag gtgccgttcc
     2701 tgggctttgc cgccctcatg cccttcgtca gcaccggacc gcgggtcgac gtgctgggcc
     2761 tgtccctctc ctcggccggg ctgctcggcg gctgggcgct cgtcgccaag ggcacgctgg
     2821 gcgtcctggc ctccctcgtt cttgccacga cgaccgagcc gcgcgacctg ctcaggggcc
     2881 tggagcgcct gcgcctgcca cggcagctga cccagatcat gggcttcatg gtccgctacg
     2941 tcgacgtcgt cggcgacgag atgcgccgga tgcgggtcgc ccgcgagtcc cgcgggttca
     3001 gcgcccgctc gccgcggcac tggggcgtcc tcgcccagtc cgccggggcg ctgttcatcc
     3061 gcgcctacga gcgcggcgag cgcgtccacc tcgcgatgct ctcgcgcggc tactccggca
     3121 cgctcccggc cggccacgac gtggccacca cccgggccga cgcggcgcgg gccgccatac
     3181 tgccggggtg cgctctgctc gtcgccctgc tcgcggtcca ggcgatgagg tgaccagccg
     3241 atgagcacgc cggtgctcga cctgcgcggg ctcgcctacg cctaccccga cgggcatcgc
     3301 gccctcaacg gcgtcgacct gcacgtccac cgcggcgaac gggtggcgct gctggggccc
     3361 aacggcgcgg gcaagacgac gctcgtcctg cacctcaacg ggatcctcac ccccggcgcg
     3421 ggctcggtcg ccgtctcggg tctgcccgtc gtgaaggcca acctcgccga ggtgcgccgc
     3481 agggtcggga tcgtcttcca ggaccccgac gaccagctct tcatgccgac cgtgcgcgac
     3541 gacgtcgcct tcgggccggc caacgcgggt cttaccggcc ccgcgctgga ccgtcgcgtg
     3601 gcggcggccc tggacgctgt cggaatgcgc gagctcgcgg accggccccc gcaccacctg
     3661 tcgttcggcc agcggcggcg ggtgtccgtg gcgaccgttc tcgcgatgga accgcggatc
     3721 ctcgtgctcg acgagccgtc ctcgaacctc gacccggcct cacgccgcga gctcgccgac
     3781 atcgtcagat ccctcgacgt caccgtgctg atggtgaccc acgacctgcc ctacgccctc
     3841 gagctgtgcg agcggtcggt catcctctcc ggcggtacca tcgtcgcgga cggcccgacc
     3901 cgggacgtgc tcgccgacac cgagctcatg accgcgcatc gcctcgagct gcccttcggc
     3961 ttcgacccga acgccaccgt gggcagccgc ggatagcatg agcggcgcgc ggaagtggtg
     4021 gaactggtag acacgcagga cttaggatcc
//
LOCUS       DATAIX010000158         4195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_242225, whole genome shotgun sequence.
ACCESSION   DATAIX010000158 DATAIX010000000
VERSION     DATAIX010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242225"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VHM65_02360"
     CDS             complement(<1..519)
                     /locus_tag="VHM65_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2704575.1"
                     /translation="MNERSPGVASSDVDDGQDAAAPAPDDDDPSLHSSSTDPGSDTSS
                     DPERPRTRRIPSAAQSVPMIRADLVDDLRALDVPEPVIDEAELVVCELVSNALRHARP
                     LPDGSIRVHWRAKAGVVELEVTDGGSDTVPCPAPISVWSSTGRGLRIVRSVAHEWGVT
                     GGSAGHTVWASLG"
     gene            complement(530..1354)
                     /locus_tag="VHM65_02365"
     CDS             complement(530..1354)
                     /locus_tag="VHM65_02365"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX2704576.1"
                     /translation="MPPRARPLVIAHRGANEVIAEHTVGAYRAAVAARADALECDVRL
                     TADRELVCLHDRTAARTSNGRAVISTQTLAELEALDWGGWKYGPGTDAGIAADPDNRR
                     LITLRQLIELAVEATRSAATGQETGLAVETKHPSRYGGQLEHAVAAMAGRFDLAAERG
                     HGVPWLRVMSSSWLAVRRWTSLRPDIPSVFLTETKIPTPARYGALPGGASAAGIDIEI
                     VRRVPDMVVRQRAHGHDVYVWTVDEPADVTLCLELAVAGIITNRPQAVMDQIAHAG"
     gene            1433..1912
                     /locus_tag="VHM65_02370"
     CDS             1433..1912
                     /locus_tag="VHM65_02370"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2704577.1"
                     /translation="MGVHLPPFQSLVDAHWYDVARLAHALAGPQDGPDVAQQAWVQAY
                     AAYPSLRSARNLRGWLLTITHRCAMDGHRARARRPVAVPDTTLAAGMPPVSAAEAPDD
                     AVWGRVAALPGRQRSAVVLRFVGDLDHAQIAAALGTTAETSRRLLSDALATLRKDLS"
     gene            1909..2502
                     /locus_tag="VHM65_02375"
     CDS             1909..2502
                     /locus_tag="VHM65_02375"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX2704578.1"
                     /translation="MTDIARSLATFAPAGLAPPVFGTADVAYVVHDSAVGRVLLAVGD
                     GGALLALVFVPGPGAEQAWLDRLGRCVSPRVLRLPRKLDDARRELDDFLAGTTRDFSV
                     ATDLRLATAFQRDVLVRLASTVGYGERSTYGALAGSLGRPTASRAVGSALGGNPLCVV
                     LPCHRIVSSTGALTGYAGGLDAKRYLLELEARHTISG"
     gene            2499..3308
                     /locus_tag="VHM65_02380"
     CDS             2499..3308
                     /locus_tag="VHM65_02380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2704579.1"
                     /translation="MRATALGLTTDLMILRLGGSEVQDCGDHLVVRSPHNPTFWWGNL
                     IALPRPPAAETVPDWLDRFTEAFPSASHRAFGVDHAPADPAGPETPWTEPWVAAGFTY
                     EPSTVLTTDSVRPPPFPNPDAVCRPLESASDWAALLEVKMANNDRHSPEGYRTFALNS
                     ITAYRRMAEGGHGQWFGAFIDGRLRSTMGLFTDGSGLARFQSVDTHPDARRQGLAGTL
                     VHHVGTWGLGELGASRLVMVADPSYVAIRLYRALGFVDAEPEHQLERPSKS"
     gene            complement(3356..3817)
                     /locus_tag="VHM65_02385"
     CDS             complement(3356..3817)
                     /locus_tag="VHM65_02385"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2704580.1"
                     /translation="MLVPALFMFVLAADGTNVVETLLVTQVLGGNGTVYGVLGVFFAA
                     GAVIGPLLAARVESDRVRVLAVVGVAGGGLNALCLTLVVTRTPDAFRGRVLATVTGGA
                     RAASLLALVIGGLLGQSLGPRHTYVALGVLTLAAAPMALLGLRGLREPRAV"
BASE COUNT          585 a         1555 c         1512 g          543 t
ORIGIN      
        1 gcccagcgag gcccacaccg tgtgtcccgc actgcccccc gtgacgcccc actcgtgcgc
       61 gacgctgcgc acgatgcgca gtccgcgccc ggtcgaggac cagaccgaga tcggcgcggg
      121 gcagggcacg gtgtcactgc ccccgtcagt cacctcgagc tcgacgactc cggccttggc
      181 ccgccagtgc acccggatgc tgccatcggg cagcggccgc gcgtgtcgca aggcgttgga
      241 gacgagctca cagacgacga gctcggcctc gtcgatgacc ggctcgggca cgtcgagcgc
      301 cctgaggtcg tcgaccagat cggcccggat catcggcacc gactgcgcgg cgctggggat
      361 gcgacgggtc cgcggccgct ccgggtcgct cgaggtgtcg gaccccgggt ccgtggagga
      421 ggagtgcagc gacggatcgt cgtcgtcggg ggcaggagcg gcggcgtcct gcccgtcgtc
      481 cacatcggag gaagccacgc ccggggagcg ctcgttcacc cgatcgaggc taacctgcgt
      541 gtgcgatctg gtccataacg gcttggggac gattcgtgat gatcccggcc accgcgagct
      601 cgaggcagag ggtcacatcc gcgggctcgt cgaccgtcca cacgtagacg tcgtgcccgt
      661 gcgcgcgctg acgaacgacc atgtcgggca ctcggcggac gatctcgata tcgatcccgg
      721 cggcgctcgc gcctcccggc agcgcgccat accgggcagg ggtgggaatc ttcgtctcgg
      781 tgaggaacac cgagggtatg tcgggacgca ggctcgtcca ccggcgcacg gccaaccagg
      841 aggacgacat gacgcgcagc cagggcacgc cgtgcccgcg ttcggcggcg aggtcgaacc
      901 ggccggccat cgcggcgacc gcgtgctcga gctgaccgcc gtatcggctg gggtgcttgg
      961 tctcgacggc gagaccggtc tcctggccgg tcgccgcgga cctcgtcgcc tcgacggcga
     1021 gctcgatgag ctgacgaagc gtgatgagcc ggcggttgtc ggggtcggcg gcgatcccgg
     1081 cgtcggtccc cgggccgtac ttccagccgc cccagtccag cgcctcgagc tcggcgaggg
     1141 tctgcgtcga gatgaccgct cgcccgttgg acgtgcgcgc cgcagtgcga tcgtgcaggc
     1201 agacgagctc gcggtcggcg gtgaggcgga cgtcgcactc gagcgcgtcc gcccgagccg
     1261 cgacagcggc ccggtaggcc ccgacggtgt gctcggcgat cacctcgtta gcaccccggt
     1321 gggcgatgac gagggggcgg gcacgcggtg gcacggcccc tatcctcaca cgagcccgcc
     1381 acggccggcc gtgacagcat cggactcaca ttcgcggcgg acggagcgtt gtatgggtgt
     1441 gcaccttcct cccttccagt cgctcgtcga cgcgcactgg tatgacgtcg cgcgcctcgc
     1501 gcacgcgctg gccggcccgc aggacgggcc cgacgtggcc cagcaagcat gggtgcaggc
     1561 gtacgcggcg tacccgtcgc tgcgttcggc caggaacctg cgcgggtggc tcctgacgat
     1621 cacgcaccgg tgtgcgatgg acggccaccg ggcgcgggcc cggcgacccg ttgcggtgcc
     1681 ggacacgacg ctggctgccg ggatgccacc ggtcagcgcg gccgaagccc cggacgacgc
     1741 cgtgtggggg cgggtcgcgg cgctgcccgg gcgccagcgc tcggccgtcg tgctgaggtt
     1801 cgtcggcgac ctcgaccatg cccagatcgc ggccgcgctc gggaccaccg ccgagacgag
     1861 ccggcgcctg ctcagcgacg ctcttgccac cctccgaaag gacctctcat gaccgatatc
     1921 gcccgatcgc tggcgacgtt cgctcccgcc ggcctggccc cgccggtgtt cgggaccgcc
     1981 gacgtcgcct acgtggtgca tgacagcgcc gtgggacggg tactgctggc ggtcggcgat
     2041 ggtggcgccc tgctggcgct ggtcttcgtc cccggcccgg gcgccgagca ggcctggctc
     2101 gaccggctcg gccgctgcgt ctcgccgcgc gtcctgcgcc tgccgaggaa gctcgacgac
     2161 gccaggcgcg agctcgacga cttcctcgcg gggaccaccc gcgacttctc ggtcgcgacc
     2221 gacctgcggc tcgccaccgc cttccagcgc gacgtcctcg tgcggctcgc ctcgaccgtg
     2281 gggtatggcg agcggtccac gtatggcgcg ctggccggct ccctgggccg ccccacggca
     2341 tcgcgggcgg tcggatcggc gctggggggg aacccgctgt gcgtggtgct gccctgccac
     2401 cgcattgtct cgtcgacggg cgccctgacc gggtatgccg gaggcctgga cgcgaagcgc
     2461 tacctgctcg agctggaggc gcgccatacc atcagcgggt gagggcaacc gcgctggggc
     2521 tgacgaccga cctgatgatc ttgcgcctcg ggggcagcga ggtgcaggac tgcggtgacc
     2581 atctcgtcgt ccggtcgccg cacaacccga cgttctggtg gggcaacctc atcgcgctgc
     2641 ctcgtccgcc agcggccgag acggtgcctg actggctcga ccggttcacc gaggcgttcc
     2701 cgtcggccag ccatcgggcc ttcggagtcg accacgcacc ggcggacccg gccggccccg
     2761 agacgccctg gaccgagccg tgggtggcgg ccgggttcac ctacgagccg tcgacggtgc
     2821 tcacgaccga ctcggtgcgg ccgccgccct tcccgaaccc cgacgccgta tgccggccgc
     2881 tggagtccgc gtcagactgg gcggcgctcc tcgaggtcaa gatggccaac aacgaccggc
     2941 actcccctga gggctaccgg acgttcgcgc tcaactcgat cacggcgtac cggcggatgg
     3001 ctgagggtgg gcacggacag tggttcgggg cgttcatcga cgggcggctc cgctcgacga
     3061 tggggctgtt caccgacggg tcgggtctgg cccgcttcca gtccgtcgac acgcaccccg
     3121 acgcacggcg ccagggcctg gccgggacgc tggtgcatca cgtcgggacg tgggggctcg
     3181 gggagctcgg cgcgtcccgg ctcgtcatgg tggcggaccc gtcatacgtc gccatccgcc
     3241 tgtatcgcgc gctggggttt gtcgacgccg agcctgagca ccagctcgaa cgaccctcga
     3301 agagctgacc ccccgtccgg agccggccgc ggtcacgggt ggggccaggg ccggctcaga
     3361 cggcgcgcgg ctcccgcagg ccacgcagac ccagcagcgc catcggcgcg gctgccaggg
     3421 tcaggacacc cagcgcgaca taggtatggc gcggcccgag cgactgcccc aggagcccgc
     3481 cgatcacgag agccagcagc gaggcggccc tggccccacc cgtgaccgtg gcgaggaccc
     3541 ggccccggaa ggcgtcgggc gtccgggtga ccaccaaggt cagacacagc gcgttgagcc
     3601 cccctccggc gactccgacg acggcgagaa cccggacccg gtcggactcc acccgagcgg
     3661 cgagcagcgg cccgatgacg gccccggccg cgaagaagac cccgagcacg ccgtacaccg
     3721 tcccgttccc accgagcacc tgggtgacga gcagcgtctc gacgacgttc gtcccgtccg
     3781 cggcgaggac gaacatgaac agcgccggca cgagcacccg cagcaccgcg tcccggcgca
     3841 gggcggccag cccccctcgg cgcgtcgacg agcagccggt ccgccggcgc cgccggagcc
     3901 agcgagccgg cccgtcgggc cgggagcacg ccgcgaccgc accgccaggg ccgccaccac
     3961 gagcagcgcg aaggtgaccg tgtcgatcgc gatcgcccag ccgtagccga cccggtcgaa
     4021 gagcacccca ccgagcgcga tccccaccag gctcgccgcg ccagccagcg aggcctgcag
     4081 cccgatggcg acgcccacca ggtcgtcacc gacgatccgc ggcacgagcg cggtccaggt
     4141 cgggccggtc accgtctggc cacactgcaa ggccaggaca cacccgatca tccag
//
LOCUS       DATAIX010000159         2744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_68672, whole genome shotgun sequence.
ACCESSION   DATAIX010000159 DATAIX010000000
VERSION     DATAIX010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68672"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VHM65_02390"
     CDS             <1..641
                     /locus_tag="VHM65_02390"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX2704581.1"
                     /translation="TVARLPEYLRVLTSLAAHGVDSVSSEDLATRAGVRSTQLRKDLS
                     HLGSYGVRGVGYDVTRLGREIARELGITQDWPIAIVGMGNLGRALAAYSGFGGGEFRI
                     VALLDEDPLVVGGHVGALAISPMAALPVIAAATGLAIGVIATPAASAQRATDDLIAVG
                     VRSILNFAPTVLAVPQGAQVRRVDLSTELHLLAFHDQRKAVSAEGAHAGVVP"
     gene            638..1933
                     /locus_tag="VHM65_02395"
     CDS             638..1933
                     /locus_tag="VHM65_02395"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX2704582.1"
                     /translation="MSVLVMGISHRTAPMTMLEVLALDDRGAAGLRERVRASATVSEV
                     LVIATCNRLEVYAEPVTFHGAVAAIGDALAASSGLPLGILRDHLYVHHEDRAVAHAFS
                     VASGLDSMAVGESQILAQVRTALSSAQRAGTAGDSLNTLFQQALRVAKKAHARTALDT
                     VSCSLVDAALAQAVEVIGPIGQAWVTVVGAGAMSSLAATSVSRAGASRLTIVNRSAAA
                     GRRVAAATGATYRPWSELTDALAGADLVISCTGAQGHVISGLAVAATRAGGRARQVYV
                     DLAMPRDVDPAVGHVAGTTLLTLADLAERLASGTPGAVVQEVEDLITGEVASYLAGRM
                     VKKVAPTVAALRTRAAQVVQAELARLDQKLPDLDPAARSELARTVHRVVEKLLHTPTI
                     RVKELSAEGGDYAEALRELFDLDPHDVAAVSVPPERGMP"
     gene            1930..>2744
                     /gene="hemC"
                     /locus_tag="VHM65_02400"
     CDS             1930..>2744
                     /gene="hemC"
                     /locus_tag="VHM65_02400"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX2704583.1"
                     /translation="MSDRAVRLGTRCSPLALVQSRTIAAALRELGCAVEVVEIVTSGD
                     ESPAPLTQIGGTGVFASAIRRAVLAGEVDLAVHSLKDLPVTPEAGLVIAAIPVREDPR
                     DALVARGGLTLGELPAGSVVGTGSPRRMAALAALGLGLEVRDVRGNVDTRIAQVDDGR
                     CDGVVLARAGLARLGRLGRVTETLDPIQMLPAPGQGALAVECRASSTALLSLLAGLDD
                     PSTRACVTAERALLGALEAGCTAPVGALAEVVEGDEGLELSLRAFVGTLDGSE"
BASE COUNT          327 a         1022 c         1008 g          387 t
ORIGIN      
        1 ccacggtcgc ccgcctgccc gagtacctgc gcgttctcac cagcctggcc gcgcacgggg
       61 tcgacagtgt ctcctccgag gacctcgcga cccgggccgg ggtgcgctcc acccagctgc
      121 gcaaggacct gagccacctc ggctcgtacg gcgtccgcgg tgtcgggtat gacgtcacgc
      181 gcctcgggcg cgagatcgcc cgtgagctcg ggatcaccca ggactggccc atcgccatcg
      241 tcgggatggg caacctcggg cgcgcgctcg cggcctactc gggcttcggg ggcggggagt
      301 tccgcatcgt cgcgctgctc gacgaggacc cgttggtcgt cggcgggcac gtcggcgcgc
      361 tggccatcag ccccatggcg gcgctgccgg tcatcgccgc cgccaccgga ctggcgatcg
      421 gggtcatcgc gacccccgcc gcctcggccc agcgggcgac cgacgacctc atcgccgtcg
      481 gggtccgctc gatcctcaac ttcgccccga ccgtgctcgc ggtcccgcag ggcgcccagg
      541 tgcgccgcgt cgacctgtcg accgagctgc acctgctcgc cttccacgat cagcgcaagg
      601 ccgtctcggc cgagggcgcc cacgcgggag tcgtcccgtg agcgttctcg tcatgggtat
      661 ctcccatcgc acggcaccga tgacgatgct cgaggtcctc gcgctggacg accgcggtgc
      721 agccgggctg cgcgagcggg tccgcgcgag tgcgacggtg tcggaggtcc ttgtcatcgc
      781 cacgtgcaac cggctcgagg tgtatgccga gcccgtcacc ttccatgggg cggtggcggc
      841 catcggcgac gcgctggccg cgtcgagcgg gctgccgctg gggatcctgc gcgaccacct
      901 ctacgtccat cacgaggacc gggccgtcgc ccacgccttc tcggtggcga gcggcctgga
      961 ctccatggcc gtcggcgagt cccagatcct ggcccaggtg cgcaccgcgc tcagcagtgc
     1021 gcagcgggcc gggacggcgg gggactcact caacacgctc ttccagcagg ccctgcgcgt
     1081 cgccaagaag gcccatgccc ggaccgcgct cgacacggtg agctgctcgc tcgtcgacgc
     1141 cgccctcgcc caggccgtcg aggtcatcgg cccgatcggt caggcctggg tcaccgtcgt
     1201 cggcgctggg gcgatgagct cgctggccgc cacgagcgtg tcgcgggccg gcgccagcag
     1261 gctcacgatc gtcaaccgca gtgccgcagc ggggcgccgg gtcgcggcgg cgacgggcgc
     1321 gacataccgg ccctggtccg agctgacgga cgcgctcgcc ggagccgacc tcgtcatcag
     1381 ctgcacgggt gcgcaggggc acgtcatctc cgggctcgct gtggccgcca cccgcgctgg
     1441 gggaagggca cggcaggtgt acgtcgacct ggccatgccg cgcgacgtcg acccagccgt
     1501 cggccacgtg gcggggacga ccctgctcac cctcgccgac cttgccgaac ggctcgcgtc
     1561 gggcacgccg ggggccgtcg tccaggaggt cgaggacctc atcaccggcg aggtcgcgtc
     1621 ctatctcgcg gggcgcatgg tcaagaaggt cgcgccgacc gtggctgccc tgcgcacccg
     1681 cgcggcccag gtcgtccagg ccgagctggc ccgcctcgac cagaagctgc ccgacctgga
     1741 ccccgcagcc cgctccgagc tggcacgcac ggtgcaccgc gtcgtggaga agctgctgca
     1801 tacgccgacg atccgggtca aggagctcag cgccgagggc ggtgactacg ccgaggcact
     1861 gcgtgagctg ttcgacctcg acccgcacga cgtcgccgcg gtatcggttc cgcccgagcg
     1921 ggggatgccg tgagcgatcg tgcggtccgc ctcggcaccc gatgcagccc gctggccctc
     1981 gtgcagtccc gcaccatcgc cgcggcactg cgtgagctcg gctgtgccgt cgaggtcgtc
     2041 gagatcgtca cctccggcga cgagtcgccc gcgcccctga cccagatcgg ggggaccggg
     2101 gtcttcgcgt cggcgatccg gcgggcggtg cttgccgggg aggtcgacct ggctgtccac
     2161 tcgctcaagg acctgccggt cacgcccgaa gccggcctgg tcatcgcggc catcccggtg
     2221 cgggaggacc cgcgggacgc cctcgtggct cgcggcggcc tcaccctggg ggagcttccc
     2281 gccgggtcgg tcgtcggtac gggctcgcct cgacgcatgg ctgcgctggc cgcgctcggg
     2341 ctgggcctgg aggtgaggga cgtgcggggc aacgtcgaca cccggatcgc ccaggtcgat
     2401 gacggccgct gtgacggtgt cgtgctggcc cgggccggcc tggcccgact ggggcggctc
     2461 ggccgggtca ccgagacgct ggacccgatc cagatgctgc ccgcgcctgg gcagggtgcg
     2521 ctggcggtcg agtgccgggc gagcagcacg gctctcctgt cgttgctcgc gggcctcgac
     2581 gacccctcga cccgggcgtg cgtcaccgcg gagcgcgccc tgctcggcgc gctcgaggcc
     2641 ggctgcaccg ccccggtcgg ggcgctcgcc gaggtcgtcg agggagatga ggggctcgag
     2701 ctgtcgctgc gggcgttcgt cgggaccctc gacgggtccg agag
//
LOCUS       DATAIX010000160         1571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_212417, whole genome shotgun sequence.
ACCESSION   DATAIX010000160 DATAIX010000000
VERSION     DATAIX010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212417"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /gene="mnmA"
                     /locus_tag="VHM65_02405"
     CDS             complement(<1..791)
                     /gene="mnmA"
                     /locus_tag="VHM65_02405"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX2704584.1"
                     /translation="MSGGVDSAVAAARMLDAGYEVVGVHLALSQNAATLREGARGCCT
                     VEDSADARRVADRLGIPFYVWDLAQRFQHDVVADFVAEYQAGRTPNPCLRCNEKIKFA
                     ALLDKARALGFDGVATGHYARVVDHPDGRRELHRAVDQAKDQSYVLGVLDADQLARSH
                     FPLGALTKAEVRAEARARGLAVAAKPDSHDICFIADGDTKGWLTRRLGQQDGDIVDTD
                     GAVVGRHTGSYAFTVGQRRGLGLGRPADGGEPRYVLRVEPDTNTVV"
     gene            complement(836..>1571)
                     /locus_tag="VHM65_02410"
     CDS             complement(836..>1571)
                     /locus_tag="VHM65_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX2704585.1"
                     /translation="MWANNEVGTVQDIPALAAIAHERGIPIHTDAVQAFGHVPVDFTA
                     ADVDLLTITAHKVGGPVGVGALVAKRTAAIVPLIHGGGQERQVRSGTLDAAGVRAFAV
                     AACEATGRREHETARLMALRDRLIKGALAIDGEIRPGGWWEAGDATRRLAGNAHLVVP
                     GCEGDSLLYLLDAAGIECSTGSACQAGVPQPSHVLLAMGLSEAEARGALRFTLGHSST
                     DADIDAVLAVLPSAVARARRASGGAA"
BASE COUNT          208 a          576 c          569 g          218 t
ORIGIN      
        1 acgacggtgt tggtatccgg ctcgacccgc aggacgtacc gcggctcccc gccgtcggct
       61 ggacgcccga gcccaagccc gcgccgctgc cccacggtga aggcatagga gccggtatga
      121 cgtcccacga cggctccgtc ggtgtcgacg atgtccccgt cctgctgacc gagccggcgg
      181 gtcagccagc ccttggtgtc gccgtcggcg atgaagcaga tgtcgtggct gtccggcttc
      241 gcggcgacgg ccagcccgcg cgcgcgggcc tcggcccgga cctccgcctt ggtcagggcg
      301 cccaggggga agtgtgaccg ggcgagctgg tccgcgtcga ggacccccag gacgtaggac
      361 tggtccttgg cctggtcgac cgctcggtgc agctcgcgcc gaccgtccgg gtggtccacg
      421 acccgggcat agtggcccgt cgcgaccccg tcgaagccga gcgcgcgtgc cttgtcgagc
      481 agcgccgcga acttgatctt ctcgttgcag cgcaggcacg ggttgggggt gcgtccggcc
      541 tggtactcgg cgacgaagtc cgcgacgacg tcgtgctgga agcgctgggc gaggtcccag
      601 acgtagaacg gtatgccgag ccggtcggcg acccgccggg cgtctgccga gtcctccacg
      661 gtgcagcatc cgcgggcacc ctcgcgcagg gtggcggcgt tctggctcag ggcgagatgg
      721 acgccgacga cctcatagcc ggcgtcgagc atccgggccg cagcgaccgc cgagtcgacg
      781 ccaccgctca tcgcggcgac cactcggcgg accggtcctg cactgaccat ggcggctacg
      841 ccgccccacc ggaggctcgc ctcgctcggg ccaccgccga gggcagcacg gcgaggaccg
      901 cgtcgatgtc cgcgtcggtc gagctgtgcc ccagggtgaa gcgcagcgca ccccgcgcct
      961 cggcctcgga cagacccatc gcaagcagga catggctcgg ctgcggcacg ccggcctggc
     1021 aggcggaccc ggtcgagcac tcgatccccg ccgcatcgag caggtagagc agcgagtcgc
     1081 cctcgcagcc gggcacgacc aggtgtgcgt tgccggccag gcgccgggtc gcatcgccgg
     1141 cctcccacca cccccctggc cggatctcac cgtcgatcgc cagggctccc ttgatgagcc
     1201 ggtcacgcag tgccatgagc cgcgcggtct cgtgctcgcg ccggcccgtg gcctcgcaag
     1261 ccgccacggc gaaggcgcgg actccggccg cgtcgagcgt gccgctgcgg acctgacgct
     1321 cctggccccc gccgtggatg agcggcacga tggctgcggt ccgcttggcg acgagggcgc
     1381 cgacccccac gggcccgccg accttgtgcg cggtgatcgt caggaggtcg acatccgcgg
     1441 cagtgaagtc gaccgggacg tggccgaacg cctggacggc gtcggtgtgg atcgggatgc
     1501 cccgttcgtg ggcaatggct gccagggccg ggatgtcctg gacggttccg acctcgttgt
     1561 tcgcccacat c
//
LOCUS       DATAIX010000161         2226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_8718, whole genome shotgun sequence.
ACCESSION   DATAIX010000161 DATAIX010000000
VERSION     DATAIX010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8718"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1635
                     /gene="gatA"
                     /locus_tag="VHM65_02415"
     CDS             109..1635
                     /gene="gatA"
                     /locus_tag="VHM65_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX2704586.1"
                     /translation="MNSDLTRASAAELAGALGRKDVSAVEATQAHLERIGAVDGPVHA
                     FLHVDAEGALATARDVDQRRAAGEDLHHLAGVPIAVKDVMATKGLPTTCGSKILAGWI
                     PPYDATLVTRLREAGMPILGKTNMDEFAMGSSTEHSAYGPTRNPWDLGRIPGGSGGGS
                     AAAVASYQAPLAIGTDTGGSIRQPASVTGTVGTKPTYGGVSRYGLVALASSLDQAGPC
                     ARTVLDAALLHAVIAGHDPMDSTSIDAPPPPVVQAALRADVTGMTVGVVRELGGEGYQ
                     PGVRARFEESVALLVEAGATVVEVSCPSFQYALAAYYLILPSEASSNLAKFDAMRYGL
                     RVTPPGDPSAEEVMAATRDAGFGPEVKRRIILGTYALSSGYYDAYYGSAQKVRRLIAD
                     DFAAAFTRADVLLSPTAPTTAFAFGDKLDDPLAMYLNDVATIPANLAGVPGMSLPSGL
                     ADEDGLPAGIQVLAPAMADDRLYAVGSALERALTSRWGGPLLAKAPALGVTAKGAADG
                     "
     gene            1628..>2226
                     /locus_tag="VHM65_02420"
     CDS             1628..>2226
                     /locus_tag="VHM65_02420"
                     /inference="COORDINATES: protein motif:HMM:NF014930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704587.1"
                     /translation="MADTVLSYADTVASYDPVLGLEVHVELGTATKMFCGCPTAFGAE
                     PNSQVCPVCLGLPGALPVVNAVAVESAIRIGLALSCTIAPWCRFARKNYFYPDMPKNF
                     QTSQYDEPIASDGWLDIELDDGSAYRVPIERAHMEEDTGKSTHVGGATGRIHGADYSL
                     VDYNRAGIPLIEIVTKPMTGTGERAPEVARAYVTALRNLL"
BASE COUNT          316 a          843 c          761 g          306 t
ORIGIN      
        1 cgcccgagcc tcaccgcgga ggaggctctc gcgggagccc cccaggcaga gcagcagcgc
       61 ttctccgtgc cccgcatcct tgacgaagcc tgaccgagga ctcatcccgt gaactccgat
      121 ctcacccggg ccagcgccgc cgagctggcc ggcgccctcg gccgcaagga cgtcagcgcc
      181 gtcgaggcga cccaggccca cctcgagcgc atcggcgccg tcgacggccc cgtccacgcc
      241 ttcctgcacg tcgacgccga gggcgcgctg gccacggccc gcgacgtcga ccagcggcgc
      301 gcggccggcg aggacctgca ccacctcgcc ggcgtcccga tcgcggtcaa ggacgtcatg
      361 gcgacgaagg gcctcccgac gacctgcggc tcgaagatcc tggcgggctg gatccctccg
      421 tatgacgcga ccctggtcac ccggctgcgc gaggccggga tgcccatcct cggcaagacc
      481 aacatggacg agttcgccat ggggtcctcg accgagcact cggcctacgg ccccacgcgt
      541 aacccctggg acctcggccg catccccggc gggtccggcg gtggctccgc ggcggcggtc
      601 gcgtcatacc aggcaccgtt ggcgatcggg accgacacgg gcggctcgat ccgccagccc
      661 gcctcggtga ccgggacggt gggcaccaag ccgacgtatg gcggggtctc gcgctacggt
      721 ctggtcgccc tggcgtcgag tctcgaccag gccgggccgt gtgctcgcac cgtgctcgac
      781 gccgcgctgc tgcatgcggt catcgccggt cacgacccga tggactcgac ctcgatcgac
      841 gccccgccac cccccgtggt gcaggctgcc ctgcgcgccg acgtgaccgg aatgaccgtc
      901 ggggtcgtgc gcgagctcgg tggcgagggc taccagccgg gagtgcgggc gcggttcgag
      961 gagtcggtgg cgctgctcgt cgaggccggc gccacggtcg tcgaggtgtc ctgcccgagc
     1021 ttccagtacg ccctcgcggc ctactacctc atcctcccga gcgaggcgtc gagcaacctc
     1081 gcgaagttcg acgccatgcg ctacgggctg cgggtcaccc cgccaggcga cccgagcgcc
     1141 gaggaggtca tggcggcgac gcgcgacgcc ggcttcgggc ctgaggtcaa gcggcgcatc
     1201 atcctcggca cctacgccct gtccagcggc tactacgacg cctactacgg gtcggcccag
     1261 aaggtccggc gcctcatcgc cgacgacttc gccgcggcct tcacccgggc cgacgtcctg
     1321 ctctcgccga ccgccccgac gactgccttc gcgttcgggg acaagctcga cgacccgctc
     1381 gcgatgtacc tcaacgacgt cgcgacgatc ccggccaacc tcgccggagt gcccgggatg
     1441 tccctgccga gcgggctcgc cgacgaggac gggctccccg cggggatcca ggtcctcgcc
     1501 ccggcgatgg ccgacgaccg cctgtatgcg gtcggttcgg cgctcgagcg ggccctgacc
     1561 tcgcggtggg gcggccccct gctggcgaag gccccggccc tcggcgtgac cgcgaagggg
     1621 gccgcagatg gctgacaccg tgctcagcta cgccgacacc gtggcgtcat acgacccggt
     1681 cctgggcctg gaggtccacg tcgagctcgg gaccgcgacg aagatgttct gcggctgccc
     1741 gacggcgttc ggcgccgagc ccaactcgca ggtctgcccg gtatgcctcg gcctgcccgg
     1801 cgccctgccg gtcgtgaacg ccgtcgccgt cgagtcggcc atccggatcg ggctcgccct
     1861 gagctgcacg atcgcgccct ggtgccggtt cgcgcgcaag aactacttct accccgacat
     1921 gccgaagaac ttccagacca gccagtatga cgagcccatc gcctcggacg ggtggctcga
     1981 catcgagctc gacgacgggt cggcataccg cgtcccgatc gagcgggcgc acatggagga
     2041 ggacaccggc aagtcgaccc acgtcggcgg tgcgacgggt cgcatccatg gtgctgacta
     2101 ctcgctggtg gactacaacc gggccgggat cccgctcatc gagatcgtca ccaagccgat
     2161 gacgggcacg ggggagcgag cgccggaggt ggctcgtgcc tatgtcacgg ccctgcgcaa
     2221 cctgct
//
LOCUS       DATAIX010000162         1785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_272143, whole genome shotgun sequence.
ACCESSION   DATAIX010000162 DATAIX010000000
VERSION     DATAIX010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272143"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(551..1627)
                     /locus_tag="VHM65_02425"
     CDS             complement(551..1627)
                     /locus_tag="VHM65_02425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2704588.1"
                     /translation="MADQPSTGMRPLNVARVIGVLERGGAQLSALRLSAALRSDGFVT
                     RLYAGDATAEGIALAAAFDVPVEAFARHAHLQWVPSTDFAEWLRPRLAGADLVHAHMF
                     GAWWAAAAAVPDGVPLVATEHNAVTWPHGDYVTEARAAAPRIAAFFTHGPAARTFADS
                     LGVPAERLFEGRSIVEGTDARPLPGLPSPRVTFAGRLHPEKGPDVLLEALAMLPDPPA
                     MLLLGDGPLRESLTARSVALGLADRVLLPGWVRSPGSYIAGATVHVVPSREEAWSQSA
                     VLALGLRVPVVGTAVEGLPATLAEGRGVLVPPENPEELAHAIDELLHGRRVPDPVPGR
                     GYAERFTPERIAPYYADTYRRLTL"
BASE COUNT          266 a          596 c          651 g          272 t
ORIGIN      
        1 ctgcccccac tgcgatgatc ttgtggcagt gagcgacctt gtcgccgaca accagttcgg
       61 cgtcacccca cgacccgtcc gattaggact tcgggagatc ccttggttac gtaaaagatc
      121 ttgatccaca ggtattgaca attcctcgcg ttgttcgggg cgctggtgtg gttcggactg
      181 ggcgtcatac gtcgtcatcg gcgccgcaag gcgcgccgcg acgaccgacg ggactgacct
      241 gcaccagact gctgttgctt ctgcctcggg ctgcgccgcg gtgagccgcc gcagaacgtg
      301 agctccggcc atgcttgtcg ggactcgatg ttcgacgtcg ctctgcacat ctacgctttc
      361 acccccgccg gtttggtacg aaaccgtcga cccggccgat caaatcactt accccgtggc
      421 aaggattact tgggttctgc ggggggactg taacccctgg gtggttcggc gaggcgtcat
      481 cgcacgatga tgacgtgcag ggtgcggggc cgtggacgag agcgggacaa cttaacaggt
      541 gggtcgttcc tcatagcgtg agccgccggt aggtgtcggc gtagtacggg gcgatccgct
      601 cgggggtgaa gcgctcggcg tatccgcgcc ccggtaccgg gtcgggaacc ctgcgaccgt
      661 gcaggagctc gtcgatggcg tgtgcgagct cttcggggtt ctcgggcggg acgagcacgc
      721 cgcggccctc cgccagcgtc gcgggcagcc cttccacagc ggtgccgacg accggcaccc
      781 gcaggcccaa tgcaaggacg gcgctttgcg accaggcttc ttcccgggac gggacgacgt
      841 gcacggtggc tccggcgata taggagccag gcgagcgcac ccatcccggc aggaggaccc
      901 gatcggccag gccgagggcg accgaccggg cagttaggga ctcccgcaac ggcccgtcgc
      961 cgagcagcag catcgccggc ggatcaggaa gcattgccag cgcctcaagc aggacgtcgg
     1021 ggcctttttc gggatgcagg cgcccggcga aggtcaccct ggggctgggc agcccgggca
     1081 gcggtcgcgc atcggtcccc tcgacaatcg accgtccctc gaacaggcgc tcggccggga
     1141 cgccgagcga gtcggcgaag gtgcgcgctg cggggccgtg ggtgaagaag gctgctatgc
     1201 gcggggcggc ggcgcgggcc tcggtgacat agtcgccgtg gggccaggtc acggcgttgt
     1261 gctcggtcgc gacgagcggc accccgtcgg gcacggccgc cgctgccgcc caccaggctc
     1321 cgaacatgtg ggcgtggacg aggtcggcgc ctgccaggcg gggacgcagc cattcggcga
     1381 aatcggtcga cggcacccat tgcaggtgcg cgtgccgggc gaaggcctcg acgggcacgt
     1441 cgaaggcggc cgcgagcgcg atcccttcgg cggtggcgtc cccggcgtac aggcgggtga
     1501 cgaaaccgtc ggaccgcagg gcggcggata gccgcagcgc ggagagctgc gctccgccgc
     1561 gctcgagcac cccgatgacc cgagcgacgt tgagcggccg catgcccgtc gacggctggt
     1621 cagccatggc cggtgccggg gccctcggcg ggcattctgg ccacggccac acccccgcgc
     1681 tcggcgctgc ggtcgaagcc gagccccgtc tctcgctccc accggcgcag aacttcctgc
     1741 tcacctgggc gttcggcgtc gtcgaggacg acggtggcgc ctggg
//
LOCUS       DATAIX010000163         4601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_8810, whole genome shotgun sequence.
ACCESSION   DATAIX010000163 DATAIX010000000
VERSION     DATAIX010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8810"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1707
                     /locus_tag="VHM65_02430"
     CDS             <1..1707
                     /locus_tag="VHM65_02430"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF017493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX2704589.1"
                     /translation="DLPDVAKGPDVTEFDYEDLLPLREDATTYRLLTSEGVRTVDGPG
                     GRTFLEIDPEALRMLTATAMHDIAHYLRPAHLGQLRMILDDPAASNNDKFVALDLLKN
                     ANIAAGGVLPMCQDTGTAIVMGKRGQHVLTPGTDERAIAHGVYDAYTRLNLRYSQMAP
                     LTMWEERNTGTNLPAQIELYADTAPGHETTYKFLFMAKGGGSANKSYLYQETKAILNE
                     DAMVRFLDEKLRSLGTAACPPYHLAIVIGGTSAELGMKTAKYASAKYLDALPTSGSML
                     AHGFRDTALEERVLELTRSFGIGAQFGGKYFCHDVRVVRLPRHGASLPVAIAVSCSAD
                     RQCLAKITPDGVFIEELETDPARFVPEVGHDDLRDSSDVVRVDLSRPMDEILAELTKH
                     PVKTRLSLIGTLVVARDIAHAKIKERLDAGEPMPQYLRDHPVYYAGPAKTPAGLPSGS
                     FGPTTAGRMDSYVAQFQAAGGSKVMLAKGNRSQQVTDACYDFGGFYLGSIGGPAARLA
                     QDCIRSVEVLEYAELGMEAVWRIEVEDFPAFIVVDDKGNDFFAEVATPAVMTIPVRPG
                     LT"
     gene            1704..2348
                     /locus_tag="VHM65_02435"
     CDS             1704..2348
                     /locus_tag="VHM65_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX2704590.1"
                     /translation="MTDRTRPQPGTRPTTPAQAWAVLTAGNARFMAGERDHPHQNVHV
                     RHRLAAKQAPFAVFFGCADSRVAAEIIFDQGLGDLFVVRTAGHVVDQAVLGSVEFGVA
                     VLQIPLVVVLGHDSCGAVSATKDAVDTGVMPRGYIRDIVERVTPSVLSATRAGRTSIE
                     EIETEHVRHTVRLLVERSHVVAERVHQGRLAIVGATYDLMDGQATLVEAIGPAT"
     gene            complement(2544..2972)
                     /locus_tag="VHM65_02440"
     CDS             complement(2544..2972)
                     /locus_tag="VHM65_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ohr family peroxiredoxin"
                     /protein_id="HEX2704591.1"
                     /translation="MPYEAVIETVYTTAATAKGGRGGHTTSADGVVDLPLGKPGSSAN
                     PKANPETLLAAGYAACFNGALNLMASREGLDTRESTVTVNVTFGKTETGVGLAVEITA
                     NVPGVDAETAQRLADRADEYCPYSKATRGNIDVTVTGVPA"
     gene            3232..4569
                     /locus_tag="VHM65_02445"
     CDS             3232..4569
                     /locus_tag="VHM65_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704592.1"
                     /translation="MTLPHHALARRSIVSLVTAGALAASLTGGAGASAAVGPAPGDSF
                     PDPAGAQIAALQKVKGSLSKAERKLSSDLSIALRSASDPSIAAALPGLRSALAKSTAG
                     TVTVDITATVTPALMSRLATVGATVAYASATAGSVRASVPVASLTTVAAWADVRSVRS
                     AATAMTANAKPTPAPTKQERVRRVEANLRAALAPSAEVGAVTSEGDATHAADAARATR
                     HVTGVGVKVCVLSDGVDSLAASRAAGELPAVDVLPGQAGAGDEGTAMLEIIHDLAPGS
                     ALGFATAFTSDASFADNIRALRRQGRCQIIVDDVIYFNEATFQDGPIAQAVIDVTNAG
                     ALYFSSAGNEGSVRSGTSANYEGRFVNSGQGIGKFIGTAHDFDPGPRVQVYEPVSFDG
                     FPAAPAILQWANPRGRGRRLRPVPLRRHGNPGGRLPGRPGRDSGPVRDPRRLR"
BASE COUNT          697 a         1689 c         1535 g          680 t
ORIGIN      
        1 gacctccccg atgtggcaaa gggacctgac gtgaccgagt tcgactacga ggacctgctc
       61 cccctgcgtg aggatgcgac gacataccgg ctgctcacca gcgagggcgt gcgcaccgtc
      121 gacggacccg gcggacggac cttcctcgag atcgaccccg aggccctgcg gatgctcacc
      181 gcaaccgcga tgcacgacat cgcgcactac ctgcgcccgg cccacctcgg ccagctgcgc
      241 atgatcctcg acgacccggc cgcgagcaac aacgacaagt tcgtcgccct cgacctgctc
      301 aagaacgcca acatcgccgc ggggggcgtc ctgccgatgt gccaggacac cggcacggcg
      361 atcgtcatgg gcaagcgcgg acagcacgtg ctcacgccgg ggaccgacga acgcgcgatc
      421 gctcacggtg tgtatgacgc ctacacgcgc ctcaacctgc gctacagcca gatggcgccg
      481 ctgacgatgt gggaggagcg caacaccggg acgaacctgc ccgcccagat cgagctgtac
      541 gccgacaccg cgccagggca cgagacgacc tacaagttcc tgttcatggc caagggcggc
      601 gggtcggcca acaagtccta cctctaccag gagaccaagg ccatcctcaa cgaggacgcg
      661 atggtccgct tcctcgacga gaagctccgc tcgctcggga ccgccgcgtg cccgccatac
      721 catctcgcga tcgtcatagg cggcacgagc gccgagctcg gcatgaagac ggcgaagtac
      781 gcgtctgcca agtacctcga cgcactgccg acatcgggct cgatgttagc ccacggcttc
      841 cgggacaccg cactcgagga gcgcgtgctc gagctgaccc ggtccttcgg catcggggcg
      901 cagttcggcg gcaagtactt ctgccacgac gtgcgcgtcg tccggctccc gcgccatggc
      961 gcatccctgc ccgtcgcgat cgccgtatcg tgctcggccg accgtcagtg cctcgccaag
     1021 atcacccctg acggcgtgtt catcgaggag ctggagaccg acccggcgcg gttcgtcccc
     1081 gaggtgggcc acgacgatct gcgcgactcg tccgatgtcg tccgagtcga cctgtcgcgc
     1141 cccatggacg agatcctcgc cgagctgacg aaacaccctg tgaagactcg gctttcgctg
     1201 attgggaccc tcgtcgtcgc ccgcgatatc gcccacgcca agatcaagga gcggctcgac
     1261 gcgggcgagc cgatgccgca gtacctccgc gaccacccgg tctactacgc cggcccggcc
     1321 aagacgcccg cgggcctgcc gtcggggtcc ttcggtccca cgaccgccgg ccggatggac
     1381 tcctacgtcg cacagttcca ggccgcgggt ggctccaagg tcatgctggc caaggggaac
     1441 cggtcccagc aggtcaccga cgcgtgctac gacttcgggg ggttctacct cgggtccatc
     1501 gggggcccgg cggcccggct cgctcaggac tgcatccgct cggtcgaggt cctcgagtat
     1561 gccgagctcg gcatggaggc ggtgtggagg atcgaggtcg aggacttccc ggccttcatc
     1621 gtcgtcgacg acaagggcaa cgacttcttc gccgaggtcg ccacgccggc cgtgatgacg
     1681 atccctgtcc gcccgggcct gacgtgaccg accggacgcg cccgcagccg ggcacccgcc
     1741 ctacgacgcc cgcgcaggcc tgggccgtcc tcactgccgg caacgccagg ttcatggccg
     1801 gcgagcgcga ccacccgcac cagaacgtcc acgtccgcca ccggctggcc gccaagcagg
     1861 cgcccttcgc cgtgttcttc ggctgcgcgg actcccgggt cgctgccgag atcatcttcg
     1921 accagggcct gggcgacctc ttcgtcgtcc ggaccgccgg tcacgtcgtc gaccaggcag
     1981 ttctcggatc ggtcgagttc ggggtcgcgg tcctgcagat cccgctggtc gtcgtcctcg
     2041 gccacgactc gtgcggggcg gtctcggcga cgaaggacgc ggtcgacacc ggcgtcatgc
     2101 cccgcggcta tatccgcgac atcgtcgagc gggtcacccc gagcgtgttg agcgccaccc
     2161 gcgcgggccg gacctccatc gaggagatcg agaccgagca cgtgcgccat acggtgcggc
     2221 tgctggtcga gcgctcccat gtcgtcgccg aacgggtcca ccagggacgg ctggcgatcg
     2281 tcggcgccac ctacgacctc atggacggcc aggcgacgct cgtcgaggcc atcggcccgg
     2341 cgacctgagg ccgctgacag gccgcccagc cgatcggtcg ttccgagcag gtgccggcgc
     2401 cctgcgcccg ttggcagccg atgaccgccg cggggttcgc gcccccgggc tgccgaggtc
     2461 gatctgaccg gccccgaccg gccgcaccca cctcagtccg ccaccccagc cacccgacag
     2521 caccagggct cccagacgct ctctcaggcc gggacgcccg tcacggtgac gtcgatgttg
     2581 ccgcgcgtgg ccttggagta ggggcagtac tcatctgccc tgtcggccag gcgctgcgcc
     2641 gtctcggcgt cgaccccagg gacgttggcc gtgatctcca cggccaggcc aaccccggtc
     2701 tcggtcttgc cgaaggtgac attgacagtg accgtcgact ccctggtgtc caggccctcc
     2761 cgggacgcca tgaggttgag ggccccgttg aagcacgcgg cgtagccggc ggccaagagc
     2821 gtctcggggt tggccttggg gttcgccgag ctgcccggct tgccgagggg aaggtcgacg
     2881 acgccgtcgg cactcgtggt gtgcccacct cggccgccct tggcggtcgc ggcggtcgtg
     2941 tagacggtct caatgacggc ttcgtacggc atacaggctc cttctgggtg tgaggtgcac
     3001 ctgctggggg atcggctcgg tgccctctga ctggttggcg tgtgcatggt tggcgtgtgg
     3061 ctgcatggcg tgcggagggc acgtgagggt gccaacccgg ttcggcgcgg tgacattccc
     3121 gccggcgctc cggttgccgg tcgccgggac cggggttacc gtcacgggac aggggcggga
     3181 ccggtcaccg ggcctacgcg cagtcccctg acgaggggaa ggctgggccc aatgactttg
     3241 ccacaccacg ctctggcgcg ccgatcgatc gtctccctcg tcaccgcagg cgcgctggcc
     3301 gccagcctca ccggcggagc cggggcgtca gcagcggtcg gccccgcccc cggcgactcg
     3361 ttcccggacc cggcaggagc ccagatcgcg gccctgcaga aggtcaaagg gtccctttcc
     3421 aaggccgagc gcaagctcag cagcgacctg tccatcgccc tgcgcagcgc gtccgacccc
     3481 tcgatcgccg cggcgctccc gggccttcgg tccgccctcg ccaagagcac agccgggacg
     3541 gtcacggtgg acatcacggc gaccgtgacc cccgctctga tgagcaggct cgcgacggtc
     3601 ggtgccactg ttgcctacgc ctcggcgacc gccggctcgg tccgcgcctc cgtccccgtg
     3661 gcgtcgctga cgacggtggc cgcgtgggcc gacgtgcgca gcgtccgtag cgccgcgaca
     3721 gcgatgacgg ccaacgccaa acccaccccg gctccgacga aacaggagcg ggtcaggcgc
     3781 gtcgaggcaa acctccgggc cgcgctggcg ccgtcggccg aggtcggcgc tgtgacgagc
     3841 gagggcgatg ccactcatgc ggcggacgcc gcccgagcca cgcgtcacgt cacgggcgtc
     3901 ggcgtcaagg tctgcgtcct gtccgacggc gtggactcac tagcggcctc ccgggcggcc
     3961 ggcgagctcc cggccgtcga cgtcctgccc ggccaagccg gtgcgggcga cgaggggacc
     4021 gccatgctcg agatcatcca tgacctcgca cccgggtctg ctctcggctt tgccaccgcg
     4081 ttcaccagtg atgcgtcctt cgccgacaac atcagggccc tgagacgtca ggggcgctgc
     4141 cagatcatcg tcgacgacgt catctacttc aacgaggcga ccttccagga cggcccgatc
     4201 gcccaggccg tcatcgacgt caccaacgcc ggcgcgctgt acttcagctc ggccggcaac
     4261 gagggcagcg tccgcagtgg cacctcggcc aactacgagg ggaggttcgt caactccggg
     4321 cagggcatcg ggaagttcat cggaacggcg cacgacttcg accctgggcc cagggtgcag
     4381 gtctacgaac cggtgagctt cgacggcttc cccgccgctc cggcgatcct gcagtgggcc
     4441 aacccccggg gccgcgggcg acgactacga cctgtacctc ttcgacgcca cgggaaccct
     4501 ggtggccgtc tcccaggacg tccaggacgg gactcaggac ccgttcgaga tcctcggcgt
     4561 ctccggtgac gacctgcgcc tcgccgtggt gaagaactcc g
//
LOCUS       DATAIX010000164         4817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_8856, whole genome shotgun sequence.
ACCESSION   DATAIX010000164 DATAIX010000000
VERSION     DATAIX010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8856"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VHM65_02450"
     CDS             <1..716
                     /locus_tag="VHM65_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704593.1"
                     /translation="TSGGAAALDVLPSTQELIDYLCPDEDVPLSTAAHISARFPYVSP
                     SGRITRGGRCAGTPGLVPGPAVSFDVDGGLFDNSGSGTVVDTWSALAPLAAQTEADQR
                     ACLVPIMVQVDNSPPAATQSSSVDARPKEPLAPVSALIGQVDSRERYSNARAAAAFSR
                     PLSAGGQTVTVSGSPATTLYFRISLYGQPGGQPPLGWTLAPQTVKDMRSQLLASANAD
                     TIRAIRALLTPGTMACTPR"
     gene            complement(792..1628)
                     /locus_tag="VHM65_02455"
     CDS             complement(792..1628)
                     /locus_tag="VHM65_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX2704594.1"
                     /translation="MDATGVPQTAAAWPSYEGAIFDLDGTIYRGDEPIPGAVDALNRI
                     AAAGARIAFVTNNAMVTAEGVAAKLAAMGYPATPGQVVTSAAATAQILLDRHGPTRVY
                     VIGAPALAKALTDAGHVVADSEVTAVVVGLDLDLTYAKLRCATTLILGGAEFVATNPD
                     RLFPLPDGLAPGGGAIVAAVATAVAHVTTPFVVGKPETPLVEQALVLLGTARDRTLLV
                     GDQIRTDILAAKRTGLYGVLVTTGAPQFGDDIAPDRVVASLSELPDPGRPTPRRRHPP
                     VE"
     gene            1747..2499
                     /locus_tag="VHM65_02460"
     CDS             1747..2499
                     /locus_tag="VHM65_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX2704595.1"
                     /translation="MTAKVALGIDIGGSGIKGAPVDLVKGELLRDRVKIATPAKSTPK
                     AVATIVGLIASEFTDDLPADAPVGVTIPGVVQHGIVRTAANIDKAWIDCPGQELFSEA
                     LGRSCHLVNDGDAAGVAEQRYGAAMDRQGLVILATLGTGIGIALLHNGVLVPNAELGH
                     LELDGYDAESRAAASAREREGLSYPVWAKRLQRYFSHLENLLWPDLFVVGGGVSRKSD
                     RFLPLLELRTPIVPALLQNGAGIIGAAALAAD"
     gene            complement(2544..3314)
                     /gene="fetB"
                     /locus_tag="VHM65_02465"
     CDS             complement(2544..3314)
                     /gene="fetB"
                     /locus_tag="VHM65_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEX2704596.1"
                     /translation="MITWWGMASAAMLLVVAAGLSALFQVGVERSILWAGARMAVQLA
                     LVGGLLTLVLAPGRSIWWSWLWVAGMVGYAADVAARRVPEVPGTRPVALAAFAAAATV
                     TLAVLFGLHVFEVAGRSVVPLAGMMVGNSLSSVVLASRRTLEEVRDRRDEVEARLALG
                     QDWQTASRPCVRAALRSALIPQIETTKATGLVFLPGAMVGLLLAGVSPQDAVMTQAVV
                     MFLVLAAVAVTSTVVALGVVRRLFTPDQRLVPIERRPG"
     gene            complement(3311..3940)
                     /locus_tag="VHM65_02470"
     CDS             complement(3311..3940)
                     /locus_tag="VHM65_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2704597.1"
                     /translation="MSPALSFEDVVVVRAGARVLDGVSFAVPSAAVTVIRGPSGSGKS
                     SLLRLANRLDVPASGVVRYRGSDTAGLDVRELRRRVGMVFQRPTAFAGTVRENLAVAR
                     AATDERMSAALAQAALGPELLSRNAAELSGGELQRVCLARTLLTGPNVLLLDEPTSAL
                     DEEAAATFEAAARGLVAGGTTALWVTHDRAQIERMAEHVLTLTDGKVHQ"
     gene            4075..>4817
                     /locus_tag="VHM65_02475"
     CDS             4075..>4817
                     /locus_tag="VHM65_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2704598.1"
                     /translation="MTTAHPGYLAARDFLLAYRADYDTALRDFQWPAVSGPFNWATDF
                     FDPLARGNAATALWIVEEDGREERYTFEEMATRSDRLAALLRDLGVGRGDPILVMLGN
                     QVELWDAMLAIMKVGAIIAPTTTALGGVDVEDRVRRAGAKGILLNPEDALKAAGLGPE
                     TLRLSVGPVDSAERGERGGTWTQATAGFHDIRQAQSREDAPACQTVTDASDPLLLYFT
                     SGTTRRPKLVEHTQVSYPVGHLSTMYWLGL"
BASE COUNT          720 a         1791 c         1681 g          625 t
ORIGIN      
        1 cgacctcggg cggcgcggcc gcgctcgacg tcctgccctc gacgcaggag ctcatcgact
       61 acctgtgtcc cgacgaggac gtcccgttgt cgaccgccgc gcacatctcg gcgcggttcc
      121 cctacgtctc accgagcggc cggatcaccc gcggcgggcg ctgcgccggc acacccggcc
      181 tggtccccgg gccggccgtg tcgttcgacg tcgacggtgg cctgttcgac aactcggggt
      241 cagggacggt cgtcgacacg tggtcggccc tcgctccgct ggcggcgcag accgaggccg
      301 accagcgcgc atgcctcgtg ccgatcatgg tccaggtcga caactcccct cccgcggcga
      361 cccagtcatc ctcggtcgac gcgcggccga aggagccgct cgcaccggtc tcggccctga
      421 tcgggcaggt cgacagccgg gagcgctact cgaacgcccg cgccgcggca gcgttcagcc
      481 gcccgctgtc ggccggtggc cagaccgtga ccgtgagcgg gtcaccggcc acgacgctgt
      541 acttccggat ctcgctctac ggccagcccg gtggtcagcc gccgctgggc tggacgcttg
      601 ctccgcagac ggtcaaggac atgcgcagcc agctgctcgc gagcgccaac gccgacacga
      661 tccgcgcgat ccgggccctg ctcacgccgg gcacgatggc gtgcacgcct cgttagcagt
      721 actggcccgc caccctccgg ttgagtgagc gcagctggtc gctatcgggg cccccaaacg
      781 accacctgcg ctcactcaac cggtgggtgg cggcggcgtg gcgtgggccg ccccgggtcg
      841 ggcagctcgg acagggacgc gacgacccgg tcgggagcga tgtcgtcgcc gaactgcggc
      901 gccccggtgg tgacgagcac gccatacagg ccagtgcgtt tcgcggcgag gatgtcagtg
      961 cgaatctggt ctcccaccaa cagggttcgg tcgcgcgccg tcccgagcag cacgagtgcc
     1021 tgctcgacca gcggtgtctc gggcttgccg acgacgaagg gggtcgtcac gtgggccacg
     1081 gcggtggcga ccgcggcgac gatggcgccg cccccgggcg cgaggccatc gggcagcggg
     1141 aagagccggt cggggttggt cgccacgaac tcggcgccgc ccaggatgag tgtggtcgcg
     1201 caccgcaact tggcgtaggt gaggtcgagg tccaggccga cgacgaccgc ggtgacctcg
     1261 gagtcggcga ccacatggcc cgcgtcggtc aacgccttcg ccagggccgg cgcgccgatg
     1321 acgtagacgc gggtcggccc gtgccggtcg agcaggatct gggccgtggc tgcggcactc
     1381 gtgacgacct ggcccggggt cgccgggtag cccatcgccg ccagcttggc ggcaaccccc
     1441 tcggccgtca ccatggcgtt gttcgtcacg aaggcaatcc gcgctccggc cgccgcgatg
     1501 cggttcagtg cgtcgaccgc gccagggatc ggctcgtcgc cccggtagat cgtcccgtcg
     1561 aggtcgaaga tcgccccctc gtatgacggc cacgccgccg cggtctgcgg caccccggtc
     1621 gcgtccacct caccgacggt agcaaacccg ctcccagcgc ggacccgatg tgtgggcccg
     1681 tgaggcgttc tcggtcctag gctaagccgc gggagctgcc caccatctct tcaggaggtc
     1741 acggacatga cggccaaggt tgcgctcggc atcgacatcg gtggttcggg gatcaagggg
     1801 gcaccggtcg acctcgtcaa gggcgagctc ctcagggacc gggtcaagat cgccactcca
     1861 gccaagtcga caccgaaggc cgtggccacg atcgtcggcc tcatcgcctc cgagttcacc
     1921 gacgacctgc cagccgacgc gcctgtcggc gtgacgattc cgggcgtcgt ccagcacggc
     1981 atcgtgcgga ccgctgccaa catcgacaag gcgtggatcg actgccccgg ccaggagctg
     2041 ttctccgagg ccctcgggcg gtcgtgccat ctcgtcaacg acggcgacgc ggcgggcgtc
     2101 gccgaacagc gctacggcgc cgccatggac cggcagggcc tggtcatcct ggccaccctg
     2161 ggcaccggga tcgggatcgc cctgctgcac aacggtgtcc tggtccccaa cgccgagctg
     2221 gggcacctcg agctcgacgg ctacgacgcg gagagccggg ccgcggcgag tgctcgcgag
     2281 cgtgaggggc tgagctaccc cgtgtgggcc aagcgcctgc agcgctactt ctcgcacctg
     2341 gagaacctgc tctggcccga cctgttcgtc gtcgggggtg gggtgtcgcg caagtcggac
     2401 cggttcctgc cgctgctcga gctgcgtacg ccgatcgtgc cggccctgct gcagaacggc
     2461 gcgggcatca tcggggctgc cgctctcgcc gccgactgag catcaccggc tcaccgacga
     2521 ccacaggcgg tcaccccggg cggtcacccc gggcgacgct cgatcggcac gaggcgctga
     2581 tccggggtga agagccgccg gaccacgccg agagccacaa cggtcgaggt gacggccacc
     2641 gccgcgagga cgaggaacat cacgaccgcc tgagtcatga ccgcgtcctg cggcgacacc
     2701 ccggccagga gcaggccgac catcgcacca gggaggaaga cgagcccggt cgccttggtc
     2761 gtctcgatct ggggaatcag ggccgagcgc aacgctgctc gcacgcaggg ccgtgacgcg
     2821 gtctgccagt cctggcccag ggcgaggcgg gcctcgacct cgtcccgccg gtcgcgcacc
     2881 tcctcgagcg tgcgccgtga ggcgaggacc accgacgaca gcgagttgcc gaccatcatc
     2941 ccggccagcg gcacgaccga gcgcccagcg acctcgaaga catgcagccc gaagagcacc
     3001 gccagagtca ccgtcgccgc cgcggcgaag gccgccagcg cgaccgggcg ggtgcccggc
     3061 acttccggca cccggcgggc ggcgacatcc gcggcatacc cgaccatgcc ggccacccag
     3121 agccacgacc accagatcga gcgtcccggc gcgaggacca gcgtgagcag gccacccacc
     3181 agggcgagct ggaccgccat ccgcgctccg gcccacagga tggaccgctc gacgccgacc
     3241 tggaacagcg ctgacaggcc cgccgcgacg acgagcagca tggcggccga ggccatgccc
     3301 caccacgtga tcattggtgc accttcccgt cggtgagcgt gaggacgtgc tcggccatcc
     3361 gttcgatctg ggctcggtcg tgggtgaccc acagagccgt cgtgccacct gcgacgaggc
     3421 cgcgcgcggc cgcttcgaag gtcgccgcgg cctcctcgtc gagcgccgac gtgggctcgt
     3481 cgagcaagag cacgttcggg ccggtgagca gggtccgggc caggcagacg cgctgcagct
     3541 cacctccgga gagctcggct gcgttgcggc tcaacagctc cgggccaagg gcggcctggg
     3601 ccagcgcggc cgacatccgc tcgtcggtcg cggcgcgagc caccgcgagg ttctcgcgca
     3661 ccgtgccggc gaacgcggtc gggcgctgga agaccatgcc gacccgccgt cgcagctcac
     3721 gcacgtcgag gccggcggta tcgctcccgc ggtagcgcac cacgcccgag gcgggcacgt
     3781 cgagccggtt ggccagccgg agcagcgagg acttccccga gcccgacggc ccacggatca
     3841 ccgtcaccgc ggcgctcggg accgcaaagc tcaccccgtc gagcacgcgg gcccccgcgc
     3901 gcaccacgac gacgtcctcg aacgacagtg cagggctcac gtcgggaggc cgggtcaccc
     3961 cgccatcctc atggacccgg cgcgggcccg ccatacccgc ggctgtgccc gagccgcgca
     4021 gatcacgcca cctcccactc gcggggcggt cgccaccctg ctagacacgg aggtatgacg
     4081 acagcgcacc ccggctacct ggctgcccgt gacttcctgc tcgcctaccg ggcggactac
     4141 gacaccgccc tcagggactt ccagtggccg gctgtcagcg gcccgttcaa ctgggcgacc
     4201 gacttcttcg acccgctggc ccggggcaac gccgccaccg ccctgtggat cgtcgaggag
     4261 gatgggcgcg aggagcggta caccttcgag gagatggcga cccgctcgga ccggctggcc
     4321 gctctcctgc gcgacctcgg cgtcggccgc ggcgacccga tcctggtcat gctcggcaac
     4381 caggtcgagc tctgggacgc gatgctcgcg atcatgaagg tgggcgcgat catcgcgccg
     4441 acgacgacgg cgctcggcgg cgtcgacgtc gaggatcgag tccgccgcgc gggtgccaag
     4501 ggcatcctgc tcaacccgga ggacgcgctc aaggcagccg ggctcggccc ggagacactc
     4561 cggctctcgg tcggcccggt cgacagtgcc gagaggggcg agaggggcgg gacctggacc
     4621 caagccacgg cgggattcca cgacatccgg caggcgcagt cacgcgagga tgcacccgcc
     4681 tgccagacgg tcaccgacgc ctcggacccc ctgctgctct acttcacctc cggcacgacc
     4741 cggcggccca agctcgtcga gcacacgcag gtgtcctacc ccgtcgggca cctgtcgacg
     4801 atgtactggc tcgggct
//
LOCUS       DATAIX010000165         2088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_158443, whole genome shotgun sequence.
ACCESSION   DATAIX010000165 DATAIX010000000
VERSION     DATAIX010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158443"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..678)
                     /locus_tag="VHM65_02480"
     CDS             complement(217..678)
                     /locus_tag="VHM65_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2704599.1"
                     /translation="MAVAFRNVELGQGTPLEHWPYEALVTVIERGTVGDWARLTRAIR
                     REPWGEVARQVEEYLGQAQPWGVGPLLQRAVDAARAEAERSERETVARHVTDLIARSG
                     LSQAEFAQRIGTSASRLSTYRTGAVVPSAALVVRMELLSARLSGVAADRLG"
     gene            complement(678..1403)
                     /locus_tag="VHM65_02485"
     CDS             complement(678..1403)
                     /locus_tag="VHM65_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704600.1"
                     /translation="MASRPAASPRRSAALAEVLASAARLQRAVPDAVLVGGAAAALYA
                     GHRDSVDHDHVLADLQDRYLEVLEAMEATEGWVTSVRASAPPLTIMGSLGGVEAGLRQ
                     LRRSRPLEVQEVDLGEAGVMRAPTIEEILRVKSYLVVLRNQVRDYLDVVAVAETTGLD
                     RAVSTLVGIDAYYVDRSGEEDSVLTALVQRLAEPNPRDHRVTRQLAAYKGLDPRWHRW
                     STVVAACQALADAIVRVVEARVP"
     gene            1601..>2088
                     /locus_tag="VHM65_02490"
     CDS             1601..>2088
                     /locus_tag="VHM65_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704601.1"
                     /translation="MDEYELLIRHTEAWAAAKKRPLDTDLLSSLLHLRDVHDRHPGTS
                     WPAGSAEHLLTVRWPGHGPLGAADAEAIVATLDTFWRFLRGTGRMASGSVEPRDLLRE
                     ARRAIPAMRARAEDPAYFGQSKALQQFGAQIGITLDGAADLDDLNARMQQIMDAWNAL
                     PQ"
BASE COUNT          318 a          751 c          722 g          297 t
ORIGIN      
        1 ctgttcaggt cgacacgcag tcggtatgtc gaggcgtgcg cgggtgccga gggaacagtg
       61 agggcgggtg gcgccccatc tgccgccgct gcccgggccc gcccgcaggg atggggccca
      121 ggcaccgtcg acggtgctgc cgccacgacg gcggtgcggt ctgcggccct tgcgggtccc
      181 ggccctctgg gcatcgaccg acacagccag aagcgctcac ccgagccggt ctgcggccac
      241 cccggacaac cgcgccgaga ggagctccat ccgcacgaca agagccgccg agggcacgac
      301 cgcgcccgtg cggtaggtcg agagccggga ggctgacgtc ccgatacgct gggcgaactc
      361 ggcctggctc agtcccgagc gagcgatgag gtcggtgacg tggcgggcca ccgtctcgcg
      421 ttcggagcgc tcggcctcag cccgggccgc atcgacagcg cgctgcaaca ggggacccac
      481 gccccacggc tgggcctggc cgaggtactc ctcgacctgc cgagcgacct ccccccaagg
      541 ctcacggcgg atcgctcggg tgagtcgggc ccagtcaccg accgtgccac gctcgatgac
      601 cgtcacgagc gcctcgtagg gccaatgttc gaggggagtc ccttgtccca gctccacgtt
      661 tcggaacgcg acggccatca cggcactcgc gcttcgacca ctcgcacgat cgcgtcggcg
      721 agggcctggc aggccgcgac gaccgtcgac cagcgatgcc agcgcgggtc gaggcccttg
      781 taggcggcca gctgccgggt cacccggtgg tcgcgcgggt tgggttcagc gagccgctgc
      841 accagggcgg tgagcacgga gtcctcctct ccggaccggt ccacgtagta ggcgtcgatg
      901 ccgaccagcg tggacacagc ccggtcgagg cccgtcgtct cggcaacggc caccacgtcg
      961 aggtagtcgc gcacctggtt acgcagcacg accagatagc tcttgacccg caggatctcc
     1021 tcgatcgtgg gcgcccgcat cactcccgcc tcgccgagat cgacctcctg cacctccagg
     1081 ggccgcgagc gccgcagctg acgcagccct gcctcgacac cacccaggct gcccatgatg
     1141 gtcagcggtg gggcactcgc gcgcacgctg gtgacccagc cctcggtggc ttccatggct
     1201 tcgagaacct cgaggtagcg atcctggagg tcggccagaa cgtggtcgtg gtcgacggag
     1261 tcgcggtgac cggcatacag tgcggccgct gcaccgccga ccaggacggc gtccggcacg
     1321 gcacgctgca gtcgcgccgc tgaggccagc acctcggcca gagcggcgct gcggcgaggt
     1381 gatgcggctg gacgagaagc catgcgttga tggtatcaga tatgataacc atcagtccga
     1441 ggatgcgacc tcgtccgctg cggtcaccct gcatccgatg ctgccagcgc gatctcggcg
     1501 tcctcgacat ccaggccacg cgtgggcacc cccgctttgc gaggcaccgt gcttggtgtc
     1561 cttgtcgcat ctcgagacga ccaagtgcca agattctcgc gtggacgagt acgagctgct
     1621 gatccgtcat accgaggcct gggcggccgc caagaagcgg cccctggaca ccgacctgct
     1681 gagcagtctc cttcatctgc gcgatgtcca tgaccgccac cccggcacgt cgtggccggc
     1741 ggggagcgcc gagcatcttc tgacggtgcg ctggcccggc cacggcccgc tcggcgctgc
     1801 ggacgccgag gcgatcgtgg cgaccctgga cacgttctgg cggttcctgc gcgggacggg
     1861 ccggatggcg tccggctccg tggagcccag ggacctgctg cgcgaggcgc gtcgcgccat
     1921 accggccatg cgtgctcgcg ccgaggaccc ggcctacttc gggcagtcca aggcgctgca
     1981 gcagttcggc gcgcagatcg ggatcaccct cgacggcgcg gccgacctgg acgacctcaa
     2041 cgcccggatg cagcagatca tggacgcgtg gaacgccctc ccccagga
//
LOCUS       DATAIX010000166         1647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_8904, whole genome shotgun sequence.
ACCESSION   DATAIX010000166 DATAIX010000000
VERSION     DATAIX010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8904"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VHM65_02495"
     CDS             complement(<1..762)
                     /locus_tag="VHM65_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704602.1"
                     /translation="MSTTPPDLMDRWATTMEQASQLARDWWDQSQAMMEPWRDVVTGM
                     ATSAEPDARHHDRHHDRHHDHDHDHDHDQHAHRGDCNDCGCHDCDGHHGRHRGRGGHQ
                     SCGCEKRDARAGSAQHHGSSCRRADADVLVRARAGERRVIPFVVRSSRRRERTVTLEV
                     GPWHACCSDELIITTAFDTASPLELGPCDERVVRLLIDIQVARRDVSVRHDSQHAETE
                     HAAAKEGTVDREAGEVVVTPRTWGGDVLCCATTYAD"
     gene            complement(759..1346)
                     /locus_tag="VHM65_02500"
     CDS             complement(759..1346)
                     /locus_tag="VHM65_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704603.1"
                     /translation="MRDGHPGVAGQASALIPPGATEQVDLTIPMDPYTEPGEYAAQLD
                     VGGVLRPVVVVVEPELAMRVSPAKVLAGPGRTSLELVVRNDGNVAIALAGTTRSWTVP
                     SGKGDADHGPEVTLRLGADDLVLEPGGMVRVTATLEVPEDLDREHRHESRVPLGLADL
                     TVLVLPRDVAPLTTPTTRSTRPARRPRTASKKETP"
BASE COUNT          206 a          564 c          616 g          261 t
ORIGIN      
        1 gtcggcgtat gtcgtggcgc agcacagcac gtcgcctccc cacgtcctcg gtgtgacgac
       61 gacctcgccg gcttcccggt cgacggtccc ctccttcgcc gcggcgtgct cggtctcggc
      121 gtgctgactg tcgtgacgga ccgagacatc gcgcctcgcg acctggatgt cgatgaggag
      181 cctcacgaca cgctcgtcgc agggcccgag ctcgaggggt gaggcggtgt cgaacgcggt
      241 cgtgatgatg agctcgtccg agcagcaggc gtgccacgga ccgacctcga gggtgaccgt
      301 gcgctcgcgc cgacgagagc tgcgcacgac aaaggggatg acgcgccgct caccggctcg
      361 ggctcggacg aggacgtccg cgtcggcccg gcggcacgaa gaaccgtggt gctgcgccga
      421 acccgctcgg gcgtcccgct tctcgcatcc gcacgactga tgcccgccgc ggccgcggtg
      481 acgtccgtgg tgcccgtcgc agtcgtgaca cccacagtcg ttgcagtcgc cgcggtgggc
      541 atgttggtcg tggtcgtggt cgtggtcgtg gtcgtggtgg cggtcgtggt ggcggtcgtg
      601 gtggcgggcg tcgggctcgg cgcttgtcgc catccccgtg acgacgtcgc gccacggctc
      661 catcatcgcc tgggactggt cccaccagtc ccgcgccagc tggctcgcct gctccatcgt
      721 cgtggcccag cggtccatga ggtcgggggg tgtggtgctc atggtgtctc cttcttcgat
      781 gcggtacgcg gacgccgggc gggccgggtc gagcgggtcg tcggtgtggt caatggggcg
      841 acgtcacgcg gcaggacgag cacggtgaga tcggcgagcc caaggggcac ccggctctcg
      901 tggcgatgct cgcggtcgag gtcctctggc acctccagcg tggccgtgac gcggaccatg
      961 cccccgggct cgaggacgag gtcgtcggcg ccgaggcgca gggtcacctc cgggccgtgg
     1021 tcggcgtcac ctttgccgct cgggacggtc cagctgcgag tcgtccccgc gagggcaatg
     1081 gcgacgttgc cgtcgttgcg gacgacgagc tcgaggctcg tccggcccgg gccggcgagc
     1141 accttcgccg gcgagacccg catcgcgagc tcgggttcga cgacgacgac gaccggccgg
     1201 agcaccccgc cgacgtcgag ctgcgcggca tactcgccgg gctcggtgta cgggtccatc
     1261 ggaatggtga ggtcgacctg ctcggtggcc cctgggggga tcagtgccga ggcctggccg
     1321 gcgacccccg ggtggccgtc gcgcacgagc accgcaccgc ggacaccgag cccggccctc
     1381 gccggtgttc tcgacggaga aggtgccctg gacccgttgt ggtggaccgc tcaacacgag
     1441 cgggcgctcg gccgtctcgg gcgtttgtct ggccatgacc tcatcctccc gctcagtcga
     1501 tcacatgcgg tcgtcggtcc gcgggagcca ggtgggtcga gctgtgcccg ccgacgctca
     1561 acgagaacag ctcggtcccg agcacggccc aggaccagcg ctgctcatag ggggagtgga
     1621 catcggtcgc cggcagcgga cccagct
//
LOCUS       DATAIX010000167         2878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_69163, whole genome shotgun sequence.
ACCESSION   DATAIX010000167 DATAIX010000000
VERSION     DATAIX010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69163"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VHM65_02505"
     CDS             complement(<1..183)
                     /locus_tag="VHM65_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704604.1"
                     /translation="MHWGRALTLLGPGPGADGERYDLLVSLAEDLRLMGDLDGARTRL
                     EEAIRIARVLGDDERAA"
     gene            complement(194..1207)
                     /locus_tag="VHM65_02510"
     CDS             complement(194..1207)
                     /locus_tag="VHM65_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704605.1"
                     /translation="MVTAVEAAELVTSVSGLDETELTETLRARTGGNPFFLVETARLL
                     AAEAHDGPGPSVAGVAAMVPDTVRDVVERRVQRLPDDTQTMLGMAAVAGPGVELAVLE
                     HATGLAADRVCTLLEPAVRSGLLVELDDRIGWRFSHALAQDAVRSTLTRSNRARLHGA
                     LAEAIEHVHDDDLDQHLDELAHHSYEAAQAGRGASALRWSVAAAESAHAHHGHDRASL
                     NWGRALTLLGPGPGADGERYDLLVSLAEDLRLMGDLDGARTRLEEAIRIARVLGDDER
                     AARAAVVFGGVTLWYWRAYGTTDWPMVEEVRRLAATAAGPRYLRAALVDCGRRVRPCP
                     PRP"
     gene            1447..1704
                     /locus_tag="VHM65_02515"
     CDS             1447..1704
                     /locus_tag="VHM65_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704606.1"
                     /translation="MLVATTITRRIAPAWIVLAGYSAATLIATGVLVPVGAGVASITN
                     FAGYVIWCLWLLAMAVILWRSAPAATATADARPRGASHVLR"
     gene            complement(1780..2253)
                     /locus_tag="VHM65_02520"
     CDS             complement(1780..2253)
                     /locus_tag="VHM65_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HEX2704607.1"
                     /translation="MTTTVDIGIPAERLDEVVSGLSRVLADSYSLYLRTHSYHWNVVG
                     PMFHSLHAMFMEEYTELSLAVDEIAERIRALGSFAPATFRQFAAMSSVPEDTDVPDAL
                     EMVRRLVSAHEATARTIRGVLPVAEGAPDQVSTDLLVRRLDVHEKTAWMLRSLLA"
     gene            complement(2250..2747)
                     /locus_tag="VHM65_02525"
     CDS             complement(2250..2747)
                     /locus_tag="VHM65_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX2704608.1"
                     /translation="MTVPSAPELLRQHHLRVTAPRVAVLDVLGERSHLTTEEVAAAVR
                     QRIGTLSMQAGYHVLDTLVAVGLLRRIEPAGSPARYERRVGDNHHHLVCRSCGIVVDV
                     DCHRQDGRCLEPTDPHGFEVDEAEVVFWGRCPDCRPAGPTPNAKTTRPTPTATTPTAT
                     PRSAR"
BASE COUNT          416 a          976 c         1067 g          419 t
ORIGIN      
        1 ggcggcgcgc tcgtcgtcgc cgaggacccg ggcgattcga atcgcctcct cgagccgggt
       61 gcgcgcaccg tcgaggtcac ccatgaggcg caggtcctcc gcgagcgaca ccagcaggtc
      121 gtagcgctcg ccgtcagcgc ccgggcccgg cccgagcagg gtgagcgcgc ggccccagtg
      181 caaggaggcc cggtcatggc cgtggtgggc atgggcggac tcggcggccg caatcgacca
      241 gcgcagcgcg gaggtatcgc gggccggctg cggtcgcggc gagccgtcgc acctcctcga
      301 ccatgggcca gtcggttgtc ccgtaggccc gccagtacca gagcgtcacg ccgccgaaga
      361 cgacggcggc ccgggcggcg cgctcgtcgt cgccgaggac ccgggcgatt cgaatcgcct
      421 cctcgagccg ggtgcgcgca ccgtcgaggt cacccatgag acgcaggtcc tccgcgagcg
      481 acaccagcag gtcgtagcgc tcgccgtcgg cgcctgggcc cggcccgagc agggtgagcg
      541 cgcggcccca gttcaaggag gcccggtcat ggccgtggtg cgcatgggcg gactcggcgg
      601 ccgcgaccga ccagcgcagc gcggaggcgc cgcggccggc ctgtgccgct tcgtagctgt
      661 ggtgggccag ctcgtcgagg tgctggtcga ggtcgtcgtc gtggacgtgc tcgatggcct
      721 cggccagtgc cccgtgcagc ctggcccggt tgctgcgggt cagtgtcgag cggacggcgt
      781 cctgagcgag ggcgtggctg aagcgccagc cgatccggtc gtcgagctcg acgagcaggc
      841 cgctgcggac cgccggctcg agcagcgtgc agacccggtc ggcggcgagg ccggtggcgt
      901 gctcgagcac ggcgagctcc actcccggtc cggccacggc cgccatcccc agcatggttt
      961 gggtgtcgtc gggcaggcgc tgaacgcggc gttcgacgac atcgcggaca gtgtccggaa
     1021 ccatcgcggc aaccccggcc accgaggggc cggggccgtc gtgggcctcg gccgccagga
     1081 gccgtgcggt ctccacgagg aagaaggggt tgcctcccgt ccgggcgcgc agcgtctcgg
     1141 tgagctcggt ctcgtcgagg ccgctgacgg aggtgacgag ctcggcggcc tcgacggccg
     1201 tgaccaccga ggcgcagccg ccggtgccgt ggtgagcgca ggaggtcggc cagcgtcgcg
     1261 tcgagcatcc ggtgtgaacc gagctcgggt ctgcggacgg tgacgatgac gacgaacggc
     1321 gtcgagccca cctgtcccat gagcaggcgc agcagctgaa gggaggcctg gtcggccacc
     1381 tgaaggtcgt cgacgaccgc cagcagcggg gagaccatcg gctacgccct caccgcggcg
     1441 ttcgccatgc tcgtcgccac caccatcacc cgccggatcg cccctgcctg gattgtcctg
     1501 gccgggtact ccgctgcgac gctcatcgcg accggtgttc tcgtccctgt cggcgccggc
     1561 gtcgcgagca tcacgaactt cgccggctac gtgatctggt gcctctggct cctggccatg
     1621 gcggtcatcc tctggcgctc ggcgccggcc gccaccgcga ccgcagacgc caggccgagg
     1681 ggcgcatccc atgtcctcag gtagcggcgc gagccggccg agggtgcgga cctgcttcgg
     1741 cgagaggctt ccgtcccgcg agctcgtccg gagcgaaggt caggcgagga ggctgcgcag
     1801 catccaggcg gtcttctcgt ggacgtcgag gcggcgcacg agcaggtcgg tggacacctg
     1861 gtccggtgcg ccctcggcca cgggaagaac accgcgaatg gtgcgcgcag tggcttcgtg
     1921 ggcggagacc aggcggcgca ccatctccag ggcgtccggg acgtcggtgt cctcggggac
     1981 cgaggacatt gccgcgaact gccggaaggt tgcgggggcg aacgagccaa gggcccggat
     2041 ccgctcggca atctcgtcga ccgcaaggga cagctcggtg tactcctcca tgaacatggc
     2101 gtggagcgaa tggaacatcg ggcccaccac gttccagtgg tagctgtggg tccgcaggta
     2161 cagggagtag gagtccgcga ggactcggga gaggcccgac acgacctcgt cgaggcgctc
     2221 cgccgggatc ccgatgtcga cagtggtggt catctggcac tccttggtgt tgctgtgggt
     2281 gttgttgcag tgggtgtcgg tcgggttgtt ttcgcattgg gcgtcggccc ggccggccga
     2341 cagtcggggc agcggcccca gaacacgacc tcagcctcgt cgacctcgaa gccgtgcggg
     2401 tcggtcggct caaggcaccg cccgtcctgc ctgtggcagt cgacatcgac gacgatgccg
     2461 caggaccggc ataccaggtg atggtggttg tcaccgaccc ggcgctcgta gcgcgctgga
     2521 ctgcccgccg gctcgatccg gcgcagcaga ccaacggcca cgagcgtgtc gagcacgtgg
     2581 taacccgcct gcatcgacaa cgtgccgatc cgctgccgaa ccgctgctgc cacctcttcg
     2641 gtggtcaggt gactgcgctc gcccaggaca tcgagaaccg cgacacgggg ggcggtcacg
     2701 cgcaggtggt gctgtcgcag gagctccggg gccgaaggca cagtcactag actagttcct
     2761 acaatgggac tattccaatg agtgactgct gaaggtttgc cgcctgggaa gacatcgcca
     2821 agctggtttt cgacacagcc ctcgacgcgc gcggaacctc gcgtcgggcg gtccagcc
//
LOCUS       DATAIX010000168         4590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_39185, whole genome shotgun sequence.
ACCESSION   DATAIX010000168 DATAIX010000000
VERSION     DATAIX010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39185"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            393..836
                     /gene="rplM"
                     /locus_tag="VHM65_02530"
     CDS             393..836
                     /gene="rplM"
                     /locus_tag="VHM65_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX2704609.1"
                     /translation="MRTYTPKPGEVTRTWHIIDATDVVLGRLASQTAVLLRGKHKPTF
                     APHVDTGDFVIIVNAAKVALTGAKADQKRAYRHSGFPGGLRSQTYAELLAKDPRKAVE
                     KAVRGMLPKNSLARQQITKLKVYSGVVHPHGAQKPVPFEITQVAQ"
     gene            965..1384
                     /gene="rpsI"
                     /locus_tag="VHM65_02535"
     CDS             965..1384
                     /gene="rpsI"
                     /locus_tag="VHM65_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX2704610.1"
                     /translation="MGGPAPKPTASAPGAATGRRKQAVARVRIVPGTGEWTINGRSLD
                     AYFPNKVHQQIVAEPFKVLELDGAYDALVRVHGGGPSGQAGAVRLGVARALNGIDEEL
                     NRPALKKAGFLTRDARVPERKKAGLKKARKAPQYSKR"
     gene            1388..2740
                     /gene="glmM"
                     /locus_tag="VHM65_02540"
     CDS             1388..2740
                     /gene="glmM"
                     /locus_tag="VHM65_02540"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX2704611.1"
                     /translation="MGRLFGTDGVRGLANRDITAELALDLSVAAAHVLADTGAFAGHR
                     AKAIVGTDGRASGEFLSAAVMAGLASAGVDVYDAGVLPTPAIAFLTGSTGADLGVMLS
                     ASHNPMPDNGIKFFAKGGHKLPDDVEDQIEARVGEAWDRPTGAAVGRVRRLLDAEERY
                     LTHLLTLVPHRLDGVRVVIDGAHGAASAVSPELFRRAGAEVSVIGAHPDGLNINDGYG
                     STHLENLRLAVLDRHADLGIAHDGDADRALAVNAEGGVVDGDQIMAILALSMREKGTL
                     AKDTLVATVMSNLGLLQAMEREGVTVLQTAVGDRYVLDEMKSGEYSLGGEQSGHVIFL
                     DHSTTGDGLLTGLMLASRVAETGTSLKDLAGVMTRLPQVMINVRDVDKDAVDTNDVVQ
                     AAVRGIGTQLDGTGRVLLRKSGTEPLVRVMVEAQTDAQARQIASSLAEVVKAELALRA
                     "
     gene            complement(2761..>4590)
                     /locus_tag="VHM65_02545"
     CDS             complement(2761..>4590)
                     /locus_tag="VHM65_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX2704612.1"
                     /translation="FQDGPIARAVIDVTNAGALYFSSAGNEGSVRSGTAANYEGRFVD
                     SGQGIGKFIGTAHDFDPGPRVQVYEPVNFDSFNAAPAILQWANPLGNAGDDYDLYLLD
                     ATGTLVAVSQDVQDGTQDPFEILGVSGDGLRLAVVKYSGAAAYLQLSTLGGRYRDAGE
                     LRAYVSPGVTRGHSAVAKAFSVAAAPAAEGIGALQPGDPDGPSGPYPGVFTRAQPPEV
                     FSSDGPRRVFYAPDGSPAPEVRQKPDITAADGVSTSLADFTPFFGTSAAAPHAAAIAA
                     LVLSGSPGATTATVRRAFSATALDLVPAGVDNRTGRGIVQADRVLRFTGATAQPLVTA
                     GTPTVVTSSDGDAYLEPGESARVTIPVTNVGDGTAPAVALNVASASPGARVAPARRAL
                     GRLIVGATRAATFTVSVPPWWPLGTPVLLTTRVTFAGAFSPATGIVSVPTGRKAAPVT
                     FSYGGEPVAIPDNDTVGASVGIDVSGIGIPSSLSFSVDGATCTTDEAATTVGIDHTFV
                     SDLVGTLSSPTGVTATVFNRSGGFGHNMCQVVFDDAAGTPFATAKASQAPFTGTWLPD
                     GPLAPLLSGSADGTWTFHVVDQARVDSGSIRSVSLHLGGYEIG"
BASE COUNT          714 a         1634 c         1586 g          656 t
ORIGIN      
        1 ggccagcatg cggatcgccc tagcgtcgag ccggacgtct gtcacctcgt cggggccgct
       61 cgccaggtcg tcgggagggt ggaccgaggg tttgtgtgcc atggcgtaca taatacgcat
      121 gcacaataga catgcatagc atgactgcac aggtttcgtg ccagttgctc tgtcggccgt
      181 atccgcgcag gtcagcgccc tcgccgccgc ccgcctgcgc cgatttgggg ccgcgtttgc
      241 cgatgggtag actaggacgc tgttgtgcca gatgcccggg tgctgggtgt ccttcgtgcc
      301 cgaacgaggt ggacgggcca gacgcgcgcc gagactgcgc acgcggcata cctcttatcc
      361 gctgagcgtc cgaagaagtg aaggtcaacc cgttgcgcac ttacaccccc aagcccggcg
      421 aggtcactcg cacctggcac atcatcgacg cgacggacgt cgtgctcggc cgcctcgcgt
      481 cgcagacggc ggtcctcctc cgcggcaagc acaagccgac cttcgcgccg cacgtggaca
      541 ccggtgactt cgtcatcatc gtcaatgccg ccaaggtcgc gctgaccggc gccaaggccg
      601 accagaagcg cgcctaccgc cactccggct tcccgggcgg gctcaggtcc cagacgtatg
      661 ccgagctcct cgccaaggac ccgcgcaagg ccgtcgagaa ggccgtccgc ggcatgctgc
      721 cgaagaactc cctcgcccgt cagcagatca ccaagctcaa ggtctactcc ggcgtcgttc
      781 acccccacgg ggcgcagaag cccgtgccgt tcgagatcac ccaggtcgct cagtaggacc
      841 gcgcagcggc acttgacccc acagctgtcc aaggagaatc gtggctgaca ccaccaccac
      901 cgacatcacc gccgccgacg acgtcgagct cacggagtac acctccgagt ccgacccgca
      961 ggtcgtgggc gggccggccc ccaagccgac agcgtccgcc cccggcgccg cgaccggccg
     1021 acgcaagcag gccgttgccc gggtgcgcat cgtcccgggc acgggcgagt ggacgatcaa
     1081 cgggcgctcg ctcgacgctt acttccccaa caaggtccac cagcagattg tcgccgagcc
     1141 cttcaaggtg ctcgagctcg acggtgcgta tgacgcgctc gtccgggtcc acggcggcgg
     1201 cccctccggc caggccgggg cggtgcgcct cggcgtggcg cgcgccctca acgggatcga
     1261 cgaggagctc aaccgtccgg ccctgaagaa ggccggcttc ctcacccggg acgctcgcgt
     1321 gcccgagcgc aagaaggccg gcctcaagaa ggcccgcaag gctccgcagt acagcaagcg
     1381 ctgacccgtg ggccggctgt tcgggacgga cggcgtgcgt ggcctggcta accgggacat
     1441 cactgccgag ctggccctcg acctctcggt tgccgctgcc cacgtccttg ccgacacggg
     1501 tgcgttcgcc gggcaccgcg ccaaggcgat cgtcggcacg gacgggcgcg cgtcagggga
     1561 gttcctgtcg gctgccgtca tggccggcct ggcttcggcg ggcgtcgacg tctatgacgc
     1621 cggcgtcctg cccacaccgg cgattgcctt cctgaccggg agcaccgggg ccgacctcgg
     1681 cgtcatgctc tcggcctcgc acaacccgat gccggacaac gggatcaagt tcttcgccaa
     1741 gggtggccac aagctccccg acgacgtcga agaccagatc gaggctcgcg tcggcgaggc
     1801 gtgggaccgg ccgaccggtg cggccgtggg ccgggtgcgt cgcctgctcg atgccgagga
     1861 gcgctatctg acgcacctgc tcaccctcgt cccgcatcgc ctcgacggcg tgcgggtggt
     1921 catcgacggg gcgcacggag cggcgagcgc cgtctcgccc gagctcttcc gccgggccgg
     1981 ggccgaggtc tcggtcatcg gggcccaccc cgacggtctc aacatcaacg acggctatgg
     2041 ctcgacgcat ctggagaacc tgcgcctcgc ggtgctcgac cgtcatgccg acctcgggat
     2101 cgcccacgat ggcgatgccg accgcgccct cgccgtcaac gctgagggag gcgtggtcga
     2161 cggcgaccag atcatggcca tcctcgccct gtcgatgcgg gagaagggca ctctcgccaa
     2221 ggacaccctc gtcgcgaccg tgatgagcaa cctcgggctg ctccaggcga tggagcgcga
     2281 gggcgtcacc gtgctgcaga cggccgttgg cgaccgctac gtcctcgacg agatgaagtc
     2341 aggggagtac tccctcggcg gcgagcagtc cggccacgtg atcttcctcg accacagcac
     2401 gaccggcgac ggcctgctca ccgggctcat gctcgcttcc cgggttgccg agaccgggac
     2461 ctcgctcaag gacctcgcgg gtgtcatgac ccggcttccc caggtcatga tcaacgtccg
     2521 ggacgtcgac aaggatgccg tcgacaccaa cgacgtcgtc caggccgcgg tgcgcggtat
     2581 cggcacccag ctcgacggca ccggccgggt gctgttgcga aagtcgggta ccgaacccct
     2641 ggtccgggtc atggtcgagg cccagaccga cgcgcaggcc cgccagatcg cgagcagcct
     2701 cgccgaggtc gtcaaggcgg aactggccct tcgcgcctag cattgcgccg catcgaccgg
     2761 tcagccgatc tcgtagccac cgaggtgaag cgacaccgac cggatcgacc ccgagtcgac
     2821 acgggcctgg tcgacgacgt ggaaggtcca cgtcccgtcg gccgacccgg acagcagcgg
     2881 ggccagcggg ccatcgggca gccacgtacc ggtgaacggc gcctggctcg ccttcgcggt
     2941 ggcgaacggc gtgccggcgg catcatcgaa caccacctgg cacatgttgt ggccaaagcc
     3001 acccgagcgg ttgaagaccg tcgccgtcac gccggtgggt gagctgaggg tccccacgag
     3061 gtcgctgacg aaggtgtggt cgatcccgac cgtggtggcg gcctcatcgg tcgtgcacgt
     3121 cgccccgtcc accgagaagg agagggacga ggggatgccg atccccgaca cgtcgatgcc
     3181 gaccgacgcg cccacagtgt cgttgtcggg tatggcgacc ggctctcctc cgtaagagaa
     3241 ggtcaccggt gcggccttcc tccctgtcgg caccgacacg atgcccgtcg ccggggagaa
     3301 ggccccggcg aacgtcaccc gggtcgtcag cagcacaggg gtgccgagtg gccaccacgg
     3361 cggtaccgaa acggtgaacg ttgccgcccg ggtggccccc acgatgagcc ggccgagggc
     3421 ccggcgcgcc ggtgccacac gtgcccctgg gctggccgat gcgacgttga gcgccacggc
     3481 gggcgccgtc ccatcgccga cgttggtgac cgggatggtg accctcgcgg actcacccgg
     3541 ctcgagatag gcgtcgccgt cgctcgacgt cacgaccgtc ggagttccgg ccgtcacgag
     3601 cggctgagcc gtggccccgg tgaagcgcag cacccggtcc gcctggacga tcccgcgccc
     3661 ggtgcggttg tcgactccag cagggaccag gtcgagcgcg gtcgccgaga aggcccgccg
     3721 gaccgtggcc gtggtggcgc cggggctgcc cgagagcacg agggccgcga tcgccgccgc
     3781 gtgcggggcg gccgccgacg tgccgaagaa cggcgtgaag tcagcgagcg aggtgctcac
     3841 gccgtcagcc gccgtgatgt ccggcttctg cctcacctcg ggggccggtg agccgtctgg
     3901 tgcgtagaag acccgtcgcg gcccgtccga gctgaagacc tcgggcggct gcgcccgggt
     3961 gaagacaccc gggtaggggc ccgaaggccc gtcggggtca ccgggctgga gagccccgat
     4021 cccctcggct gcgggcgcgg ccgcgacgct gaacgccttg gccaccgcgg agtggccccg
     4081 ggtcactcct ggtgacacgt aggccctgag ctcgccggcg tcgcggtaac ggccccccag
     4141 ggtgctcagc tggaggtagg cggcggcgcc ggagtacttc accacggcga ggcgaaggcc
     4201 gtcaccggag acgccgagga tctcgaacgg gtcctgggtc ccgtcctgga cgtcctggga
     4261 gaccgccacc agggttcccg tggcgtccaa gaggtacagg tcgtagtcgt cgcccgcgtt
     4321 cccgaggggg ttggcccact gcaggatcgc cggagcggcg ttgaagctgt caaagttgac
     4381 cggttcgtag acctgcaccc tgggcccagg gtcgaagtcg tgcgccgttc cgatgaactt
     4441 cccgatgccc tgaccggagt cgacgaacct cccttcgtag ttggccgcag tgccactgcg
     4501 gacgctgccc tcgttgccgg ccgagctgaa gtacagcgcc ccggcgttgg tgacgtcgat
     4561 gacggcccgg gcgatcgggc cgtcctggaa
//
LOCUS       DATAIX010000169         3945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_39201, whole genome shotgun sequence.
ACCESSION   DATAIX010000169 DATAIX010000000
VERSION     DATAIX010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39201"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VHM65_02550"
     CDS             <1..643
                     /locus_tag="VHM65_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX2704613.1"
                     /translation="LGSGNVFTQESLLFSKTAVITFGGAYAVLAYVSQQAVQTYGWIS
                     ASDMVTGLGLAETTPGPLIMVVQFVGFLAAYNHPGSLPPLFAGVLGASITVWVTFLPC
                     FFFIFLGAPYVEKLRHSQALGHALTGIGAAVVGVILNLALWFALNTAFATVTVTWWGP
                     VQLFVPRWPTVVWASLAITALAAVLVFRFKISTLRVLAACAAVGVAAAVTGLT"
     gene            684..3314
                     /gene="mgtA"
                     /locus_tag="VHM65_02555"
     CDS             684..3314
                     /gene="mgtA"
                     /locus_tag="VHM65_02555"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEX2704614.1"
                     /translation="MAATSSPLSVADGPLSIQIAATESPERVLGLLGSGPRGLTPSEV
                     DERSRTFGPNAVRSHHAGAWSVLAGQLASPLLWLLIGAAMVSALVGERIDATIIGAIL
                     LASVGLGFANEYKAARAAEALHSEIRHVVTVVRDGAPASVEVTHLVPGDVVHLGVGSI
                     VPADLRLLAADNLECDESILTGESVPAPKSAPAVAGATALADLSCCLFMGTVVHEGAA
                     DAVVVATGRLTQFGRIAVGLGEKHPQTEFQIGLTAFSGLLARVGGALSAVIFAVNIAL
                     GRPVIEAVLFSLAVAVGITPQLLPAVVSTSLATGSRALARKKVLVKRLVCIEDLGDID
                     VLFTDKTGTLTEGHISFERATDALGADSPDVRRLGLLCNEASVSAGTAVGGNPLDVAL
                     WEAPGSTDVPMTDVERVDVAPFDHDRRCVSVLLDLGAERMLVTKGAPESVLERCADVP
                     AEARRVLEREFAAGNRVVAVATRPAPELATLSPADEHDLRLAGYLTFLDQPKDSAAHS
                     IARLAGLGITVKIVTGDNPVVAETVCRTLGLTSQGTLTGAELDALDDAALEERVTQTT
                     IFARVSPEQKARILSAQRQAGSAVAFLGDGVNDALALHHADVGISVDSAIDVAKDAAD
                     IILLERDLDVLADGVGEGRRIFANTIKYVLMGTSSNFGNMASVTVAAAFLPFLPLLPF
                     QVLLNNLLYDTSQMAIPTDRVDPEQLARPSHWDIAFVRRFMIRFGPISSVFDFATFAV
                     MLWGFHAAAPLFRSGWFVESLATQTLIVFVIRTHRVPFFRSRPAPALLVSVVAVVLIG
                     ALIPQSPLNETLGFAPLPFAFFAVLVGFVLTYLLCVEGAKLLFFRVHPTTIARPLQRG
                     DTHRVHRIAARWSHHEPLPR"
     gene            complement(3414..3791)
                     /locus_tag="VHM65_02560"
     CDS             complement(3414..3791)
                     /locus_tag="VHM65_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX2704615.1"
                     /translation="MTSTSRHGPDAGPPAVNEVHLVGRVSGPPDERVLPRGDELIALR
                     IVVPRVGGSRRGAPTGPKGPKVDTIDATCWSASTRRVACRLAVGDTVQVAGALRRRFF
                     RTGAGAQSRYDVEVTSLRRVASA"
BASE COUNT          514 a         1481 c         1286 g          664 t
ORIGIN      
        1 gttgggcagc ggcaacgtgt tcacccagga gagcctgctg ttctccaaga ccgccgtcat
       61 caccttcggc ggcgcctacg cggtgctggc ctacgtctcc cagcaggcgg tgcagaccta
      121 tggctggatc agcgccagcg acatggtcac cggcctcggc ctggccgaaa ccaccccggg
      181 gccgttgatc atggtggttc agttcgtggg cttcctcgcc gcctacaacc accccggcag
      241 cctcccaccg ctgttcgcag gggtcctggg ggcgtcgatc acggtgtggg tcaccttcct
      301 gccctgcttc ttcttcatct tcctcggcgc cccctacgtc gagaagctcc gccacagcca
      361 ggccctgggc catgcactca ccgggatcgg cgccgccgtc gtcggagtga tcctgaacct
      421 ggcgctgtgg ttcgcgctga acaccgcctt cgccaccgtc accgtcacct ggtggggacc
      481 ggtgcagctc ttcgtaccac gatggccgac cgtggtctgg gcatcactgg cgatcaccgc
      541 cctcgccgcg gtcctggtat tccggttcaa gatcagcacc ctgcgggtcc tggccgcgtg
      601 tgcggcggtg ggtgtcgccg ctgccgtcac cggcctcacc tgaccggggc aggccacgtc
      661 cctcggtcaa tagaatggcc ggtatggcgg cgacgagctc cccactgtcc gtcgctgacg
      721 gccccctctc gatccagatc gcggcaaccg agtcgcccga gcgcgtgctc gggctgctgg
      781 gttccggtcc gcgcggcctg accccgtccg aggtcgacga gcggtcgcgc acgttcggcc
      841 ccaacgcggt ccggtcgcac catgccggcg cgtggtcggt ccttgccggc cagctcgcca
      901 gcccgctgct gtggcttctt atcggcgccg cgatggtgtc ggccctcgtc ggtgagcgga
      961 tcgacgccac gatcatcggg gcgatcctgc tcgcgtcggt gggtctgggg ttcgccaacg
     1021 agtacaaggc ggcgcgtgcc gccgaggcgc tgcactcgga gatcaggcac gtcgtgaccg
     1081 tcgttcgcga tggggcgccg gccagcgtcg aggtcaccca cctcgtcccc ggcgatgtcg
     1141 tccatctcgg cgtcgggtcg atcgtccccg cggacctgcg gctgctcgct gcggacaacc
     1201 tcgagtgcga cgagtccatc ctcaccggcg agtcggtccc cgcgcccaag tcggccccgg
     1261 cggtcgccgg ggccacggca ctggccgacc tgtcgtgctg cctcttcatg ggtaccgtgg
     1321 tgcacgaggg cgcggccgat gcggtggtgg tcgcgaccgg gcggctcacc cagttcgggc
     1381 ggatcgcggt cggcctcggg gagaagcacc cgcagaccga gttccagatc ggcctcacgg
     1441 ccttctccgg gttgttggcc cgggtcggtg gagcgctcag cgcggtcatc ttcgccgtga
     1501 acatcgccct ggggcgcccg gtcatcgagg ccgtgctctt ctcgctcgcg gtcgccgtgg
     1561 ggatcacccc ccagctgctc cccgccgtcg tgtccaccag cctcgccacc gggtcgcgcg
     1621 cgctcgcgcg caagaaggtc ctcgtcaagc gactggtctg catcgaggac ctcggcgaca
     1681 ttgacgtgct gttcaccgac aagacaggga cgctgaccga ggggcacatc agcttcgagc
     1741 gggccaccga tgccctcggg gccgacagcc cggacgtgcg ccgcctcgga ctgctgtgca
     1801 acgaggcctc cgtgtccgca gggaccgcgg tcggcggcaa cccgctcgac gtggcgctct
     1861 gggaagcgcc cgggtcgacg gatgtcccca tgaccgacgt ggagcgggtc gacgttgctc
     1921 ccttcgacca cgatcgccga tgcgtctcgg tgctgctcga cctgggtgcc gagcggatgc
     1981 tggtcaccaa gggcgccccc gagtcggtgc tcgagcggtg cgcggacgtg cccgccgaag
     2041 cccggcgggt gctcgagcgt gagttcgcgg ccggcaaccg ggtcgtggcg gttgcgaccc
     2101 ggcccgcccc tgagctggcc acgctctccc ccgccgacga gcacgacctg cgtctcgcgg
     2161 gctacctgac cttcctcgac cagccgaagg actcggcggc ccactcgatc gcccggctgg
     2221 cgggcctcgg catcacggtc aagatcgtca cgggcgacaa ccccgtcgtt gccgagaccg
     2281 tatgccggac cctcggcctc acctcccagg ggacgctcac cggggccgag ctcgacgccc
     2341 tcgacgatgc cgcgctcgag gagagggtca cccagacgac gatcttcgcc cgcgtcagcc
     2401 ccgagcagaa ggctcgcatc ctgagcgccc agcgccaggc cggctccgcc gtcgccttcc
     2461 tcggtgatgg cgtcaacgac gcgctcgcgc tccatcacgc cgacgtgggc atctcggtcg
     2521 actcggccat tgacgtcgcc aaggacgcgg ccgacatcat cctgctcgag cgcgacctcg
     2581 acgtgctggc cgacggtgtt ggcgaagggc ggcggatctt tgccaacacc atcaagtacg
     2641 tcctcatggg gacgtcgtcg aacttcggta acatggccag cgtcaccgtc gccgcggcgt
     2701 tcctgccgtt cctgccgttg ctgcccttcc aggtgctgct caacaacctg ctctacgaca
     2761 cgagccagat ggcgatcccc accgaccggg tggacccaga gcagctcgcc cgcccctcgc
     2821 actgggacat cgccttcgtc cgccggttca tgatccgctt cgggccgatc agctcggtct
     2881 tcgacttcgc caccttcgcc gtcatgctgt ggggcttcca cgccgctgcc ccacttttcc
     2941 gctcgggctg gttcgtcgag tccctggcca cccagaccct tatcgtgttc gtcatccgca
     3001 cgcatcgcgt cccgttcttc cgcagccggc ctgcgccggc cctgctcgtc tcggtcgtcg
     3061 cggtcgtgct gatcggcgcg ttgatccccc agagcccgct caacgagacg ctcgggttcg
     3121 caccgcttcc cttcgccttc ttcgccgttc tcgtggggtt cgtcctcacc tacctgctgt
     3181 gcgtcgaggg cgccaagctt ctcttcttcc gggtccaccc gaccaccatc gcccggccgc
     3241 tgcagcgtgg ggacacccat cgcgtgcacc gaatcgccgc ccgatggagc caccacgagc
     3301 cactcccccg ctgaggctca ccggcggagg ctgatgctga ccacacgggc gtagacgcgt
     3361 ctgccgtcaa tcgtcctccc gactcgggat gggtccccag cacccgcgcg tgctcacgca
     3421 ctggcgaccc gccgcagcga ggtgacctcg acgtcatacc ggctctgcgc tccggcacca
     3481 gtgcggaaga agcggcggcg cagcgcaccg gcaacctgca cggtgtcacc gaccgcaagc
     3541 cggcatgcga cccggcgggt cgaggccgac cagcaggtcg cgtcgatcgt gtcgaccttc
     3601 ggccccttcg gccccgtcgg cgccccacgg cgcgagccgc ccacgcgtgg caccacgatc
     3661 cgcagagcga tgagctcgtc accgcggggt agcacccgct cgtcaggtgg cccactgaca
     3721 cgcccgacca gatgcacctc gttgacggcg ggagggccgg cgtccgggcc gtggcgcgac
     3781 gtggaggtca tgccccgagc ctcccccgcc gggtccgggt tgtcgctcgc ctaggctcct
     3841 cctgtggagg atgggacttc ccaacgggga gatgtggaca gtcgcaccgg cccgttggct
     3901 caggcgggcc ggtgcgactg tccacatctc cccgttggga agtcc
//
LOCUS       DATAIX010000170         3028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_69582, whole genome shotgun sequence.
ACCESSION   DATAIX010000170 DATAIX010000000
VERSION     DATAIX010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69582"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..2199)
                     /locus_tag="VHM65_02565"
     CDS             complement(175..2199)
                     /locus_tag="VHM65_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX2704616.1"
                     /translation="MTPVETGLVLQLGLAAVAVVAALAAPQRARSLAAGGASVALGVA
                     GAVTGALVLGGGTGSLEVPLAVAPGLVAELTLAPDRLGGLFMILAAAVSAVAALFGIG
                     YAHGPAASRTGWTSYALFVLGLQLVPAAGDVVAFLFAWELMAIGSTVLLFADHDRRAQ
                     VRSAGLWYAVMTHLSFLLLLAGFAVLGNAAGSTQFSAMAHASVGASAASGPAFVLLTL
                     GFATKAGVVPMHVWLPRAHPEAPSHVSAVMSAAMVKMGVYGVLLTVTRLMPDGPQWWG
                     LLLLALALPSALYGILQASVASDLKRLLAYSTTENVGLILTAVAISMLARASGAPAVA
                     GVALVAALMLAMSHAAFKTVLFLGAGAILHATGERDLDRLGGLAAGMPVTSAAFAVAA
                     LGAAALPVTSGFAAEWVLLQALIHGHGSVAPGQGDRLTTALLPAAVAVVALTAGLALM
                     TFVKAYGIAFLARPRSAGAAAAREPSVSMRMAMLAGAAAVLGLGLAPGLAAAAAASAV
                     GTSEARAVGMAGVDLPGLGALLDPMALTLLGAIVTVPVLALTWALARRQARRPVDLPW
                     GCGGVRVGPRMQYTATSFAEPLVRVFDDALQPTRDLQVTHVRESRYLVEEVRYRQSVD
                     DIVEHRVYVPLVRLLDRVGDAARSVQNGSIYRYLGFSFAALVLILLVVVR"
     gene            complement(2196..2732)
                     /locus_tag="VHM65_02570"
     CDS             complement(2196..2732)
                     /locus_tag="VHM65_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704617.1"
                     /translation="MPVPLYRARTETFRTPMNPLRLLRAIRKAGRVAEPAPPAPEGAP
                     VGDPSLAGSVHVRHVDAGSCNGCEIEVGAAFGPVYDVERYGARLVASPRHADVVLVTG
                     VVTHNMAEPLRRTVDATPRPRVVLAVGDCARDCGAFAGGYGVAGAVGDIVPVDLEVPG
                     CPPEPDQIVAALRRVTRR"
BASE COUNT          473 a         1083 c         1158 g          314 t
ORIGIN      
        1 gtggctcctt gccggcgagc ttgaccaggt cacgccaggg ctggccggcg cctggcccga
       61 cgcggccttc gaggcgggcg cgcacctggc gcatgacgcc gacgagcagc ggagccacgg
      121 cgagcagggt caggcactgg acggcgatga gcagggccgc ccgaagcgtc gtcgtcaccg
      181 gaccaccacc agcagtatga gaaccagagc ggcgaaggag aacccgaggt agcggtagat
      241 gctgccgttc tggaccgacc gggccgcgtc gccgacccgg tcgagcagcc gcacgagcgg
      301 cacatagaca cggtgctcga cgatgtcatc gacgctctgg cggtagcgga cctcttcgac
      361 gaggtagcgc gactcccgga catgggtgac ctgcaggtcc cgggtcggct gcagcgcgtc
      421 gtcgaagacc cgcaccaggg gctcggcgaa cgacgtggcg gtgtactgca tccgggggcc
      481 gacccggacg ccgccgcacc cccacggcag gtcgaccggg cgccgagcct gccgtcgggc
      541 cagggcccag gtgagcgcga ggacgggcac ggtgacgatg gccccgagca gggtcagggc
      601 catcgggtcg aggagcgctc cgagtccggg caggtcgaca ccagccatcc cgaccgcgcg
      661 cgcctcggac gtcccgaccg ccgaggcggc cgcggcggcc gccagtcccg gagccagccc
      721 gagaccgagg acagcggccg ctccggcgag catcgccata cgcatcgaca cactcggctc
      781 gcgggccgcg gcggcaccag ccgagcgcgg ccgggcgagg aacgcgatcc cgtaggcctt
      841 gacgaaggtc atcaacgcga gcccggcggt gagcgcgacc accgccacgg ccgccgggag
      901 aagggccgtc gtgagccggt cgccctgccc cggcgctacc gacccgtgcc cgtggatcag
      961 ggcctgcagg agcacccact cggcggcaaa gcccgacgtg accgggagcg ccgccgcccc
     1021 gagcgcagcc accgcgaaag ccgccgacgt gaccggcata ccggcagcga ggccgccgag
     1081 acggtcgagg tcgcgctcgc cggtggcgtg caggatggcc ccggccccga ggaagagcac
     1141 cgtcttgaag gccgcgtggc tcatcgcgag catgagcgcc gcgacgaggg cgaccccggc
     1201 cacggccggc gcacccgacg cgcgggccag catcgagatg gcgacggcgg tgaggatgag
     1261 cccgacgttc tcggtcgtcg agtaggccag cagccgcttg aggtcgctgg cgaccgacgc
     1321 ctgcaggatc ccgtagagcg ccgacggcag cgcgagcgcg agcagcagca gcccccacca
     1381 ctgcggcccg tcaggcatga gccgggtcac cgtgagcagg acgccgtaga cgcccatctt
     1441 gaccatggcg gcactcatga ccgccgacac gtggctcggg gcctcagggt gcgcacgcgg
     1501 cagccagacg tgcatcggga cgacacccgc cttcgtcgca aagccgagcg tgagcaggac
     1561 gaaggcgggg ccggaggcgg cgctggcgcc caccgaagcg tgggccatgg ccgagaactg
     1621 cgtgctgccg gcggcgttcc cgagcacggc gaagccggcg agcagcagca ggaagctgag
     1681 gtgggtcatc acggcatacc agagcccggc cgagcgcacc tgcgcgcgcc ggtcgtggtc
     1741 ggcgaagagc aggaccgtcg acccgatcgc catgagctcc caggcgaaga ggaacgccac
     1801 gacatcaccg gccgccggga cgagctgcag gccgaggacg aacagcgcgt atgacgtcca
     1861 gccggtgcgc gaggcagcgg gcccgtgggc gtagccgatg ccgaagaggg ccgcgacggc
     1921 gctgaccgcg gcggcgagga tcatgaacag gccgccgagg cggtccggtg cgagggtcag
     1981 ctcggcgacc aggccggggg ccacggcgag cgggacctcg agggagccgg tgccgccgcc
     2041 gagcacgagc gcgccagtga cggctccggc cacgccgagg gcgaccgacg cgccaccggc
     2101 tgcgagcgac cgggcgcgct ggggtgccgc gagggcggcc accacggcca cggcggccag
     2161 gcccagctgc agcaccaggc cggtctcgac cggggtcatc ggcgggtgac ccgcctcagg
     2221 gcggcgacga tctggtccgg ctccggcggg cagccgggga cctccaggtc gacggggacg
     2281 atgtcgccca cagcgccggc gacgccatac cccccggcga aggcgccgca gtcgcgcgcg
     2341 cagtccccga cggcgaggac gaccctgggc cggggcgtgg cgtcgacggt gcgccgcagc
     2401 ggctcggcca tgttgtgcgt gacgaccccc gtgacgagga cgacgtccgc gtgcctcggt
     2461 gaggcgacga ggcgggcgcc ataccgctcg acgtcgtaga ccgggccgaa cgccgccccg
     2521 acctcgatct cgcagccgtt gcaggagccc gcgtcgacgt ggcggacgtg gacgcttccg
     2581 gcgagcgagg ggtcgccgac cggggcaccc tccggtgcgg gcggggcggg ttcggcgacg
     2641 cgtccggcct tgcggatcgc ccgcaggagg cgcagcgggt tcatgggcgt acggaacgtc
     2701 tcggtcctgg cccggtagag cgggacgggc acgatgaccc tccctcggta tgccgggtgg
     2761 ctgggtgtgt cgttcctccg gtgcgcagtt tcgcagatcc acctgcggac tcgccatccg
     2821 gggcggccac gagggagcgg ctccgcgtca tacgcacgaa ccgccgtggt cagccctggt
     2881 cagccctcgt cagcgccccg acctgctcga tgtatgacga tcgggcctgc tcgggcgagg
     2941 tccccttcag cgccgcccag gcgtcgtact tggcccggcc gacgaagtcc atcatcccgg
     3001 gacggcgacc gcttgcgtcc ccctgggt
//
LOCUS       DATAIX010000171         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_69589, whole genome shotgun sequence.
ACCESSION   DATAIX010000171 DATAIX010000000
VERSION     DATAIX010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69589"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VHM65_02575"
     CDS             complement(<1..509)
                     /locus_tag="VHM65_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX2704618.1"
                     /translation="MNWIEAARHLRERREPGVLVTLVTVRGHAPREAGAKMVVAASQQ
                     WGSIGGGNLEATAVDRARAMLAAKATRPEMLAQELTDRATVAFGQQCCGGTTTVLLDP
                     LPTVAAVAIFGMGHVGREVARILARHDLELHLVDAREAHASPTSLPALDDACARVHWH
                     HAPWPDPLLP"
     gene            complement(506..>2142)
                     /locus_tag="VHM65_02580"
     CDS             complement(506..>2142)
                     /locus_tag="VHM65_02580"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX2704619.1"
                     /translation="FAALAALGATLTGRPVRVRLNRALDLTMTGKRHGFHARWQAGFD
                     DEGHIVALDTTLTADGGWSLDLSEPVLGRALCHVDNAYWIPHVRVHGQIAQTNKTSQT
                     AFRGFGGPQGMLVIEDVIGRCAPLMGLDPATVRERSFYSSGQETPYGQEVRHPERMHR
                     IWAQLLASADVARRRVEVDEFNAAHRHTKRALGITPVKFGISFNLTAFNQAGALVLVY
                     KDGSVLVNHGGTEMGQGLHTKMAQVTATALGIPLSLVRIAPTRTDKVPNTSATAASSG
                     ADLNGAAVKNACEQIRERLVEVAARHFAVPAGAVRLADAGASAEGSTRPPLAWADLVR
                     QAYFRRVQLSALGFYRTEGLAWDPVAMRGQPFKYFAYGAAVTEVEVDGFTGANLTRRV
                     DIVHDVGDSLSPLVDVGQIEGGFVQGAGWMTLEELLWDERPGTSLGRLLTASASTYKL
                     PSLSEMPRVFNVALFEGATEDGVVYGSKAVGEPPLMLAISVREALRQAVAAFGAPGTS
                     VDLALPATPEAVFWAVEAVRNGTTDGGVTRTADGTP"
BASE COUNT          291 a          762 c          787 g          302 t
ORIGIN      
        1 ggtagaaggg ggtccggcca gggtgcgtga tgccagtgca cccgcgcaca cgcgtcgtcg
       61 agcgccggca ggctggtcgg cgacgcgtgt gcctcgcggg cgtcgaccag gtgcagctcg
      121 aggtcgtgcc gggcgaggat ccgggcgacc tcgcgtccca cgtggcccat gccgaagatc
      181 gcgacggcgg cgacggtcgg cagggggtcg agcaggacgg tcgtcgtccc gccgcagcac
      241 tgctgcccga aggcgaccgt ggcacggtcg gtgagctcct gggcgagcat ctcgggccgg
      301 gtcgccttcg cggcgagcat ggcccgggcc cgatccaccg ccgtcgcctc gaggttgccg
      361 ccgccgatcg agccccactg ctgcgacgcc gcgacgacca tcttggcgcc ggcctcgcgg
      421 ggtgcgtgac cccggacggt gacgagggtg accaggacac ccggttcgcg gcgctcgcgc
      481 aggtgccggg cagcctcgat ccagttcacg gcgtgccgtc cgcggttctg gtgacccctc
      541 cgtcggtggt gccgttgcgc actgcctcga ccgcccagaa gaccgcctcc ggcgtggcgg
      601 gaagggcgag gtcgacgctc gtgccggggg cgccgaaggc cgccaccgcc tgcctcaggg
      661 cctcgcgcac cgagatcgcg agcatgagcg ggggttcgcc gacggccttg ctcccgtaga
      721 cgacgccgtc ctcagtggcg ccctcgaaca gcgcgacgtt gaagacccgc ggcatctcgg
      781 acagactcgg cagcttgtac gtgctggccg acgccgtgag cagccggccc aggctcgttc
      841 caggccgctc gtcccagagg agctcttcca gggtcatcca cccggcaccc tgcacgaagc
      901 cgccctcgat ctggccgacg tcgaccagtg gcgagaggct gtcgccgacg tcgtgcacga
      961 tgtcgacccg gcgggtgagg ttcgccccgg tgaacccgtc gacctcgacc tccgtcaccg
     1021 cggcgccata cgcgaagtac ttgaacggct ggccacgcat ggcaacgggg tcccaggcca
     1081 gcccctcggt gcggtagaag cccagggccg agagctggac gcgccggaag tacgcctgcc
     1141 ggaccaggtc ggcccacgcg agcggcggcc gggtggaacc ctcggcggac gcccccgcgt
     1201 cggcgagccg gacggcgccg gcgggcacgg cgaaatgcct cgccgcgacc tccaccagtc
     1261 gctcacggat ctgctcgcag gcgttcttga cggccgcacc gttgaggtcg gcgcccgagc
     1321 tcgccgccgt cgccgaggtg ttcggcacct tgtcggtgcg cgtcggcgcg atgcgcacga
     1381 gggagagggg gatcccgagc gccgtggcgg tgacctgggc catcttcgtg tgcagcccct
     1441 ggcccatctc ggtgccgccg tggttgacca ggacggagcc gtccttgtag acgaggacga
     1501 gcgcgcccgc ctggttgaac gccgtgaggt tgaacgagat cccgaacttc accggggtga
     1561 tccccaacgc ccgtttggta tgccggtgcg cggcgttgaa ctcgtcgacc tccacccggc
     1621 gccgggccac gtccgccgag gcgagcagct gggcccagat ccgatgcatt cgctcggggt
     1681 ggcggacctc ctggccgtac ggcgtctcct ggccggagga gtagaagctc cgctcgcgca
     1741 cggtggccgg gtccagaccc atcagcggcg cgcagcgtcc gatgacgtcc tcgatgacga
     1801 gcatcccctg cgggccgcca aatccgcgga aggcggtctg cgaggtcttg ttggtctgcg
     1861 cgatctgccc gtgcacgcgg acgtggggga tccagtacgc gttgtcgaca tggcacagcg
     1921 cccggccgag gacgggttcg gacaggtcga ggctccagcc gccgtcggcc gtcagcgtcg
     1981 tgtcgagcgc gacgatatgt ccctcgtcgt cgaacccggc ctgccaccgc gcgtgaaagc
     2041 cgtgtcgctt gccggtcatc gtcaggtcga gcgcgcggtt gagccggacg cggaccgggc
     2101 gccccgtcag ggtcgcgccg agcgccgcga gcgccgcgaa gc
//
LOCUS       DATAIX010000172         4092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_9450, whole genome shotgun sequence.
ACCESSION   DATAIX010000172 DATAIX010000000
VERSION     DATAIX010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9450"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VHM65_02585"
     CDS             complement(<1..492)
                     /locus_tag="VHM65_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2704620.1"
                     /translation="MRTAIAVEWLKLRRSRVVVVTTAVLAGLLPVLAAGFMAAYLTGG
                     DSQLAVKARGSVSGSTWWDYLSLDAQLLSVGALLGVGVVVCWTYGREFSDGTVAGLFA
                     LPVSRAQIAWAKALVVLGWGLATSVLAALVAIPLGVLLDLGWPSGWASLATRPSGSAS
                     RVER"
     gene            complement(492..1244)
                     /locus_tag="VHM65_02590"
     CDS             complement(492..1244)
                     /locus_tag="VHM65_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2704621.1"
                     /translation="MSEPRRPTGPVALDARDVRRGYRDVLAVDGVDIRVESGEILALV
                     GLNGAGKTTLMRLLLAMVRPDSGSVRVLGREARACTAEQWSRVGHLIEAPFAYPELTV
                     TENVVVAARLHGLSAPAAAQAAEHVLARLALGSLATRRSRTLSLGNRQRLGLACAMAH
                     DPDVLVLDEPTNALDPRGVLVVRAALRDAAQRGAAVLVSSHHLDEVARVADRIAVMHR
                     GRIIGALDPAGHDLERAFFDMIYAAEPEEVDA"
     gene            complement(1237..1806)
                     /locus_tag="VHM65_02595"
     CDS             complement(1237..1806)
                     /locus_tag="VHM65_02595"
                     /inference="COORDINATES: protein motif:HMM:NF037625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2704622.1"
                     /translation="MTERSERTRARLQNAALELFLEHGYDETTVAQIAAAVGVSQMTF
                     FRHFPTKESVVLDDPYDPLIAAAVLAQRSDLPAFERVRRGLREAWRRLPEPAEEQTRD
                     KIRLAVRHPALRAATFENNHRTEAIIVEALESAGVPRMDAVVAAGACLGAIMGALVDW
                     GTSGDDAPLGERVLAALACLPHEGNDPDE"
     gene            complement(1823..3511)
                     /locus_tag="VHM65_02600"
     CDS             complement(1823..3511)
                     /locus_tag="VHM65_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704623.1"
                     /translation="MAARHGGQPTYRLSVELVDGDMVLDRWERSIGFRDIRLDTEPDG
                     LGAPFTIVVGGQPVFVRGVNWVPDDLFVSALTPQRYRARLSEAKAAGATLVRVWGGGI
                     YEDDAFYDACDELGLMVWQDFLFACAAYPEEEPHRSEVEEEAREAVTRLMPHPSLVLW
                     NGNNENLWGWFDWDWQPRLVGRTWGRGYYLDLLPRVVAETDPTRPYWPGSPWSGGFGR
                     HPNDDAYGLVHIWDVWNTRDYTDYRRRVPRFVAEFGYQAPPTWATLARAVHDDPLAPG
                     SPGMLHHQKADDGNGKLARGLSAHFAEPASFEDWHLLTQMNQARAVQTGVEHFRSHRG
                     RCMGTIWWQLNDCWPVTSWSVIDGDGRRKPAWYALRAAYAPCLVTIQPRGEGLAVVLV
                     NDSPHEWSGSLVVARRSVTGLLREEQSWPVAVAAYGASTIPLPARLAQPDDTRTEVLV
                     ADVGDRRAWWWFERDLDLDLPAPVLSAVATATERGYRVDVSARTLVRDLCLFTDRLAP
                     DAVVDDMLVSLLPGESARFDVRTASDLDVGALTSAPVLRCLGDGAGGGLVNPEC"
BASE COUNT          591 a         1467 c         1511 g          523 t
ORIGIN      
        1 cctttcgacc ctgctggcgc tcccgctcgg cctcgtcgcg agcgaggccc acccggaggg
       61 ccacccgagg tcgaggagga ctccgagcgg gatggccacg agggcggcga gtaccgacgt
      121 cgcgaggccc catccgagca cgacgagcgc cttcgcccag gcgatctgcg cacggctcac
      181 gggcaacgcg aagagccctg cgacggtgcc gtcggagaac tcgcgtccgt acgtccagca
      241 cacgacgacg ccgactccga gcagggcgcc gaccgagagc agctgggcgt cgagcgacag
      301 gtagtcccac cacgtcgacc cgctcactga gccgcgagcc ttgaccgcga gctggctgtc
      361 gccgccggtg aggtacgcgg ccatgaagcc ggcggcgagc acgggcagga gcccggccag
      421 gacggcggtc gtcacgacga cgaccctcga tcggcgcagc ttgagccact ccacggcgat
      481 ggcggtgcgc atcaggcgtc gacctcctcg ggttcggcgg cgtagatcat gtcgaagaag
      541 gcccgctcga ggtcgtgacc ggccgggtcg agggcgccga tgatccggcc ccggtgcatg
      601 accgcgatcc ggtccgccac ccgggccacc tcgtcgaggt gatggctcga cacgaggacg
      661 gccgcccccc gctgggcggc gtcccgcagg gccgcccgga cgacgaggac gccgcgcggg
      721 tccagggcgt tcgtcggttc gtcgaggacg agcacgtccg ggtcgtgggc catcgcgcac
      781 gcgaggccga gccgctggcg gttgccgagc gagagggtgc gcgaccgtcg ggtcgccagc
      841 gagccgagcg cgagtcgggc aaggacgtgc tcggcggcct gggctgcggc cggcgcggac
      901 agcccgtgca ggcgcgcggc gacgacgacg ttctcagtca cggtcagctc ggggtaggcg
      961 aacggcgcct cgatgagatg cccgacccgg gaccactgct cggcggtgca ggcgcgcgcc
     1021 tcgcgtccga ggacccggac cgaccccgag tcggggcgca ccatcgcgag gagaagtcgc
     1081 atgagggtcg tcttgccggc cccgttgagc ccgacgaggg cgaggatctc cccggactcg
     1141 acccgtatgt cgaccccgtc cacggcgagg acgtcccggt agccgcggcg cacgtcgcgc
     1201 gcgtcgagcg cgacggggcc cgtcggccgg cgcggctcac tcatcggggt cgttgccctc
     1261 atgcgggagg caggcgaggg ccgcgaggac ccgttccccg aggggcgcgt cgtccccgct
     1321 cgtcccccag tcgacgaggg cgcccatgat cgcccccagg caggcgcccg cggccacgac
     1381 cgcgtccatc cggggcacgc ccgcgctctc gagggcctcg acgatgatcg cctcggtgcg
     1441 gtggttgttc tcgaaggtcg ccgctcgaag tgccgggtgt cgcaccgcga ggcggatctt
     1501 gtcccgggtc tgctcctcgg cgggctcggg cagccggcgc cacgcctcgc gaagaccgcg
     1561 ccggacccgc tcgaaggcgg gcaggtcgct tcgctgcgcg agaacggccg cggcgatgag
     1621 cgggtcgtag gggtcgtcga ggacgaccga ctccttcgtc gggaagtgcc ggaagaaggt
     1681 catctgcgag acgccgaccg ccgccgcgat ctgggcgacc gtcgtctcgt cgtaaccgtg
     1741 ctcgaggaac agctcgagcg ccgcgttctg gaggcgggct cgggtgcgct cggatcgttc
     1801 cgtcacccct gaatgttagt cactaacatt cagggttgac cagacccccg cctgccccgt
     1861 cgccgagaca ccgcagcacg ggtgctgagg tcagcgcacc gacgtcgagg tcgctcgccg
     1921 tgcgcacgtc gaagcgcgcg gactcccccg gcagaaggct cacgagcatg tcgtcgacga
     1981 cggcgtcggg cgcgagccga tcggtgaaca ggcacaggtc ccgcacgagc gtccgcgcgc
     2041 tgacgtcgac ccggtagccc cgctccgttg ccgtcgcgac agcggagagg acgggtgccg
     2101 gcaggtcgag gtcgaggtcc cgctcgaacc accaccaggc gcgccggtcg cccacgtcgg
     2161 cgacgaggac ctcggtgcgg gtgtcgtcgg gctgggcgag ccgggccggt agcgggatgg
     2221 tcgaagcgcc atacgcggcg acggcgaccg gccacgactg ctcctcccgg agcaggcccg
     2281 tgacgctgcg gcgggcgacg accagcgacc ctgaccactc gtgcggcgag tcgttgacga
     2341 ggacgacggc gagcccctcg ccgcggggct ggatggtgac caggcagggg gcgtaggcgg
     2401 cgcgcagggc gtaccacgcc ggcttacgcc gtccgtcccc gtcgatgacc gaccacgacg
     2461 tgaccggcca gcagtcgttg agctgccacc agatcgtccc catgcagcga ccccggtggg
     2521 agcggaagtg ctccactccg gtctggactg cgcgggcctg gttcatctgg gtgagcaggt
     2581 gccagtcctc gaacgacgcg ggttcggcga agtgcgcgga caggccgcga gcgagcttgc
     2641 cgttgccatc gtcggccttc tggtggtgga gcatcccggg agagccgggc gcgagcgggt
     2701 cgtcatggac agcgcgggcc agcgtcgccc acgtcggcgg cgcctggtag ccgaactccg
     2761 cgacgaaccg cgggacgcgg cgccggtagt cggtgtagtc cctcgtgttc cacacgtccc
     2821 agatgtggac cagcccgtag gcgtcgtcgt tggggtggcg accgaacccc cccgaccacg
     2881 gggagccggg ccagtacggc ctcgtcgggt ccgtctcggc gacgacccgc gggagcaggt
     2941 cgaggtagta acccctcccc cacgtacggc ccacgagccg cggctgccag tcccagtcga
     3001 accagcccca caggttctcg ttgttgccgt tccacaggac gaggctcggg tggggcatga
     3061 gccgggtgac ggcctcgcgg gcctcctcct cgacctcgct tcggtgcggc tcctcctccg
     3121 ggtaggccgc gcacgcgaag aggaagtcct gccacaccat gaggccgagc tcgtcacacg
     3181 cgtcatagaa ggcgtcgtcc tcgtagatgc ccccgcccca gacccggacg agggtggcgc
     3241 cggccgcctt cgcctcgctc aggcgcgcgc gatagcgctg gggcgtcagc gcggacacga
     3301 agaggtcgtc ggggacccag ttgaccccgc ggacgaagac gggctgcccg ccgacgacga
     3361 tggtgaaggg cgccccgagg ccgtccggct cggtgtcgag ccggatatcc cggaagccga
     3421 tgctgcgctc ccagcggtcg aggaccatgt ccccgtcgac gagctcgacc gacagccggt
     3481 atgtcggctg ccccccatgt cgtgcggcca ccactggagc gggtcggcga ccgccagctc
     3541 gaccgggtcc ccgacgccgg ccgttcccgt ggactcggtc ccgtgcccac gcaccgcggc
     3601 gcggacggcg agtggggccg gcccacggtc cccgggcgcg tcggcgacga cgctgacgtc
     3661 gatgcgcacc cggccggccg gcccgtcgac gctcacctgg gaacggaccg cgccgaggcg
     3721 ggccgtggac cagccgacga gagagaccgc cttccagatg cccgaggtga ccgtcgaggg
     3781 gccccagtcc cagccgaagc tcgccgccat cttccggacg aagttgaacg gggccgggta
     3841 ggcgttcggg agggcgccga gccgcgccgc ctcggcatac gcccacgtcc acgccgacgt
     3901 gaaggtgacc cgcagctcgt tggccgtctc gcgcaggagc gccgtgacgt cataccggca
     3961 ggcgcggtgc atgttgcgcg acgtcccgag caccgtcccg ttgaaggcga cctcggcgat
     4021 cgtgtcgagc ccgtcgaaga cgagctcgta ccgggcgcag ccctgcccct cgtggtcgaa
     4081 caccgtcgtg ta
//
LOCUS       DATAIX010000173         1935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_9539, whole genome shotgun sequence.
ACCESSION   DATAIX010000173 DATAIX010000000
VERSION     DATAIX010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9539"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1434
                     /locus_tag="VHM65_02605"
     CDS             91..1434
                     /locus_tag="VHM65_02605"
                     /inference="COORDINATES: protein motif:HMM:NF005913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX2704624.1"
                     /translation="MTREQAQPAVTTASDEVVRICQDLIRIDSSNYGDGSGPGERAAA
                     EYVMTELTEVGLDPVLVESAPGRSSVLVRIEGADPARPGLVVHGHLDVVPAEPADWQV
                     DPFGGEIRDGCVWGRGAVDMLDMVAMVLANVREMARTGMRPARDLTVAFFADEEAGGV
                     YGSHWVTEHHPEYFQGATEAISEVGGFSVTVPSAGTGEPTRAYLIQTAEKGIAWLRLH
                     AHGRAGHGSLPHSESAIVRLAEAIARIDAHPWPREYIASVRSLLDGLSEITGTAYDDA
                     APDALLAQLGTAALFVRATLQDTANATMLTGGYKHNVVPQRASAALDARFLPGHEDDL
                     VATLRELAGEHVTVEVEHRDIALEAPFDTDLVSAMSRALRREDPAAAVLPYCLSGGTD
                     NKALHGLGITGYGFAPLRLPADLEFVPMFHGVDERVPLDSLTFGARVLGSFLRDC"
     gene            complement(1414..1545)
                     /locus_tag="VHM65_02610"
     CDS             complement(1414..1545)
                     /locus_tag="VHM65_02610"
                     /inference="COORDINATES: protein motif:HMM:NF040150.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 family protein"
                     /protein_id="HEX2704625.1"
                     /translation="MRDYEYRVLTFVPSVSRPDLRRELTEFAEYGHWERARSAVSQE"
     gene            1584..>1935
                     /locus_tag="VHM65_02615"
     CDS             1584..>1935
                     /locus_tag="VHM65_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704626.1"
                     /translation="MTPDPRAALAVLVSALERHLEAAASRRDPDDPTVTAAAQDLAEA
                     FDAYDEALFDSTEVATPLAIYDGPFEDDDSEDDDEDDEDDDDDEDDDDEDLDDDDEDT
                     GIYSGLEDVDLDDDS"
BASE COUNT          302 a          709 c          655 g          269 t
ORIGIN      
        1 ccggagacaa tagcacgcag gcgaggtgtt cgccgaaccg gtcgtgcctg cccgcccccc
       61 tccgtcgacg ggtgggttta tggtgccggt atgacgcgtg agcaggccca gccggccgtc
      121 accaccgctt cggacgaggt cgtgcggatc tgccaggacc tcatccggat cgactcgagc
      181 aactacggcg acgggtcggg cccgggggag cgagcggctg ccgagtacgt catgaccgag
      241 ctgaccgagg tcggcctcga cccggtcctc gtcgagagcg cgccgggccg atcgagtgtc
      301 ctggtgcgga tcgagggcgc cgaccccgcc cgcccgggtc tggtcgtgca cggccacctc
      361 gacgtcgtcc cagccgagcc ggccgactgg caggtcgacc ccttcggggg cgagatccgc
      421 gatggctgcg tctggggacg cggggccgtg gacatgctcg acatggtcgc gatggtgctg
      481 gccaacgtcc gcgagatggc ccgtacgggg atgcggcccg ctcgggacct caccgtcgcg
      541 ttcttcgccg acgaggaggc cgggggcgtc tacggcagcc actgggtcac cgagcaccac
      601 ccggagtact tccagggcgc caccgaggcg atcagcgagg tcggcggctt cagcgtcacc
      661 gtcccctccg ccggcaccgg cgagcccacc cgcgcctacc tgatccagac cgccgagaag
      721 ggcatcgcct ggctccgcct gcacgcccac gggcgcgccg gccatggctc cctcccgcac
      781 tcggagagcg caatcgtccg gctcgccgag gccatcgccc ggatcgatgc ccacccgtgg
      841 ccccgcgagt acatcgcctc ggtccgctcc ctgctcgacg gtctcagcga gatcaccggc
      901 acggcgtatg acgacgccgc gccggatgcg ctgctggctc agctcggcac ggcggcgctc
      961 ttcgtccggg cgaccctgca ggacacggcg aacgcgacaa tgctcaccgg cggctataag
     1021 cacaatgtcg tgccgcagcg cgcgagtgca gcgcttgacg cccggttcct gcccgggcac
     1081 gaggacgacc tcgtggcgac cctgcgcgag ctggccggcg aacacgtcac ggtcgaggtc
     1141 gagcaccgcg acatcgcgct cgaggcccct ttcgacaccg acctcgtctc ggcgatgagc
     1201 cgggcactgc gccgcgagga cccagccgcg gccgtcctgc cctactgcct ctcgggcggc
     1261 accgacaaca aggccctgca cggcttgggg atcaccggct acgggttcgc tccgctgcgc
     1321 ctgcctgccg acctggagtt cgtgccgatg ttccacggcg tcgacgagcg ggtcccgctc
     1381 gactcgctga ccttcggagc cagagtgctc gggtcattcc tgcgagactg ctgaccgcgc
     1441 ccgctcccag tgcccatact cggcgaactc ggtgagctcg cgtcgcaggt ctggccggga
     1501 gacgctcggg acgaaagtga ggactcggta ctcgtagtca cgcatcgctg cccattgtgg
     1561 accaaaccag ataccgtcga gtcatgaccc cggacccgcg tgccgcgctc gccgtcctgg
     1621 tatcggccct tgagcggcac cttgaagcag ccgcctcgcg gcgggacccc gacgacccca
     1681 ccgtcacggc agcggcccag gatctggccg aggcgttcga cgcgtacgac gaggcactct
     1741 tcgactcgac cgaggtcgcg acaccgctcg cgatctacga cggccccttc gaggacgacg
     1801 actccgagga cgacgacgag gacgacgagg acgacgacga cgacgaggac gacgacgacg
     1861 aggacctcga cgacgacgac gaggacaccg ggatctactc gggcctcgag gacgtcgatc
     1921 tcgatgacga ctccg
//
LOCUS       DATAIX010000174         2580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_69707, whole genome shotgun sequence.
ACCESSION   DATAIX010000174 DATAIX010000000
VERSION     DATAIX010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69707"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VHM65_02620"
     CDS             complement(<1..415)
                     /locus_tag="VHM65_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704627.1"
                     /translation="MSEVLVLVETTPQGVRKATLELLTLARRLGEPAAVLCGAADDAM
                     VSALGEYGASVVYVVPGTEEFLSVPAAEALVEIAGRAQPAAILITSGPTGKDVAARAA
                     VRLDSGIITDAVDVQAGDGGLTATQSVFAGTWHVSS"
     gene            complement(530..1309)
                     /locus_tag="VHM65_02625"
     CDS             complement(530..1309)
                     /locus_tag="VHM65_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX2704628.1"
                     /translation="MKIVVLVKYVPEPTATWRFAPDLTLDRGAVEGRLSELDEYAVEQ
                     AVRLVEGGLTAEIVYLTVGPAKAVDGLRKALSIGGDSAVHVNDAAIHGSDSLATSLVL
                     AKALESIGYDLVMAGMASTDAEMSVVPSMVADRLGINQATFAGQLTVDGSTVSIRRDS
                     DTASEEVSTSLPAIVSVTDQNPEARYPAFRAIMQSKKKPVTTMTLADLGLDASQVGLG
                     ASATKVLSVDRSPARQAGALVTDDGTGATQLADFLASKGFV"
     gene            complement(1581..>2580)
                     /locus_tag="VHM65_02630"
     CDS             complement(1581..>2580)
                     /locus_tag="VHM65_02630"
                     /inference="COORDINATES: protein motif:HMM:NF019436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704629.1"
                     /translation="AVARVNARTAAAETPRTAEPRSLTEHPRTHLALAMLNAMHGDLL
                     DDLDSPLTLTMALRHTRRDVSLNREALAATYPHAAGTLVVFLHGLAENDDSWAYRAQE
                     RFGDAGTTYGTLLEHDLGVTALYVRYNTGLRISANGALLAELLGRLRDAWPAPVEQIV
                     LVGHSMGGLVIHSALFVDDPWAELVSHTITLGAPHLGAPLEQAVTRLAHGVPRSATLR
                     VMRQILRGRSAGIKDLRHGNLVHADWHGHDPDDPSNRRVAVRPHPAPRHHAIVSTVTK
                     DPGHAVGAVLGDLMVLPHSAGGLETPDDQICRIGGLHHLDLLNHPSVYAQILTWLR"
BASE COUNT          367 a          851 c          966 g          396 t
ORIGIN      
        1 ggctcgacac gtgccacgtc ccggcgaaga cggactgcgt cgcggtgaga ccaccgtcgc
       61 cggcctggac gtcgaccgcg tcggtgatga tcccggagtc gagccggact gcggcacggg
      121 cggcgacgtc cttgcccgtc ggacccgagg tgatgaggat cgccgcgggc tgggcccggc
      181 cggcgatctc gacgagggct tcggccgccg ggaccgagag gaactcctcg gtgccgggga
      241 cgacgtagac gacggacgcg ccatactcgc ccagcgcact gaccatggcg tcgtccgcgg
      301 cgccgcacag gacggcggcg ggctcgccga ggcggcgggc aagggtgagc agctcgagag
      361 tggccttgcg gacaccctgc ggggtggtct cgacgaggac gaggacttcc gacacggttc
      421 cttctcctgt tacttgtctg gcggtggctg gggaatggct ggtggtcact ggtggtggcg
      481 acatctcaca ccagggcgac gcgggatggc gcgcgggtgg ccgcgagggt cagacgaagc
      541 ccttgctggc gaggaagtcg gcgagctggg tcgcgcccgt cccgtcgtcg gtgacgagcg
      601 caccggcctg gcgagccgga gaccggtcga ccgagaggac cttggtcgcg gaggcgccga
      661 ggccgacctg ggaggcgtcg agcccgaggt cggccagcgt catcgtcgtg accggcttct
      721 tcttgctctg catgatcgcc ctgaacgcgg ggtatcgggc ctcggggttc tggtcggtca
      781 ccgacacgat cgccgggagg ctcgtgctga cctcttcgct cgcggtgtcg ctgtcgcgcc
      841 ggatcgagac cgtcgagccg tcgacggtga gctgcccggc gaacgtcgcc tggttgatcc
      901 cgagccggtc ggcgaccatc gagggcacga cggacatctc ggcgtcggtc gaggccatgc
      961 cggccatgac gaggtcgtag ccgatcgact ccagggcctt ggcgaggacg agcgaggtgg
     1021 ccagcgagtc cgagccgtgg atcgccgcgt cgttgacgtg gacggccgag tcgccaccga
     1081 tcgacagcgc cttgcgcagc ccgtcgacgg ccttggccgg gcccaccgtg aggtagacga
     1141 tctcggccgt cagcccaccc tcgacgaggc ggacggcctg ctcgacggca tactcgtcga
     1201 gctcggagag gcgtccttcg acggcaccgc ggtcgagggt caggtctggg gcgaaccgcc
     1261 aggtggcggt cggttcgggc acgtacttga ccagcacgac gatcttcatg ggtcctgcct
     1321 tctcgctcgg tcgttcgatt cacggggcca ggcgcatgct acggcccggc gcggacacgc
     1381 tccacgtcag agcctgccct gaactggcct gttcgcacag ggacttgggg ccctagttta
     1441 ctgagcaagg cgacccgcct gcctggcgga gagcctcgac ccgctccggt ggcggcaccg
     1501 gagatcatca tcatcgtggt gatggggctt ctcggccaca cgagagtgcg actgcgacgg
     1561 cgacggccac gtccccggcc tcagcgcagc caggtcagga tctgggcgta gaccgacggg
     1621 tggttgagca ggtccaggtg gtggagccca ccgatgcggc agatctggtc gtcgggagtc
     1681 tccagtccac ccgcgctgtg cggcagcacc atgaggtcgc cgaggacggc ccccacggcg
     1741 tgacccgggt ccttggtgac cgtgctgacg atggcatggt gccgaggcgc cgggtgcggg
     1801 cgcacggcca cccgccggtt cgacgggtcg tcgggatcgt ggccgtgcca gtcggcgtgg
     1861 acgaggttcc cgtgccgcag gtccttgatc ccggcgctgc gaccccgcag gatctggcgc
     1921 atcacccgca gggtggcgct gcgcggaacg ccgtgggcga gccgggtcac ggcctgttcg
     1981 agcggcgcac ccaggtgcgg cgcacccagg gtgatggtgt ggctgacgag ctcggcccac
     2041 ggatcgtcga cgaagagcgc actgtggatc accagcccgc ccatcgagtg gcccacgagg
     2101 acgatctgct cgaccggggc aggccaggcg tcacggagcc gcccgagcag ctcggccagc
     2161 agtgccccgt tggccgagat ccgcaacccc gtgttgtacc gcacatagag agcggtgacg
     2221 cccaggtcgt gctcgagcaa cgtgccgtac gtcgtccccg cgtccccgaa ccgctcctgc
     2281 gcccgatagg cccatgagtc gtcgttctcg gccagcccgt gcagaaagac cacgagcgtc
     2341 ccggccgcgt gcgggtaggt cgcggcaagg gcctcgcggt tcagtgacac atcgcggcgg
     2401 gtatgacgca gcgccatcgt cagcgtgagg ggtgagtcca ggtcgtcgag caggtccccg
     2461 tgcatcgcgt tgagcatcgc gagcgcgaga tgggtcctcg ggtgctcggt gaggcttcgc
     2521 ggttccgcgg tgcgtggcgt ttcggccgcc gcggtccttg cgttcacccg ggcgactgca
//
LOCUS       DATAIX010000175         2829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_159112, whole genome shotgun sequence.
ACCESSION   DATAIX010000175 DATAIX010000000
VERSION     DATAIX010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159112"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VHM65_02635"
     CDS             <1..355
                     /locus_tag="VHM65_02635"
                     /inference="COORDINATES: protein motif:HMM:NF025196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HEX2704630.1"
                     /translation="LAKDPDAGLLGYHEWLGRRTLLLSYWRSPEHLMRFAANPDGPHV
                     GPWRDFMRRAGSNPAVGIWHETYRIPVGHSEAIYANMPEFGLAKATTRVPIVPGTQTA
                     RARFSFRAPPSSAGA"
     gene            complement(318..821)
                     /locus_tag="VHM65_02640"
     CDS             complement(318..821)
                     /locus_tag="VHM65_02640"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEX2704631.1"
                     /translation="MAEVFRVTFVCSGNICRSPMGEVILRRLAQGAGMAGLVEVDSFG
                     TGGWHEGQPAHRPTLTALRHHGYDGSAHRARQVTSDVFETHDLVLAADEGHVRQLQRL
                     ARTPGHRAALRLMREFDPSAVAAGTLEVDDPYYGDAADYERCLQEVEAACRGLLTHLR
                     STVVPGS"
     gene            complement(826..1539)
                     /gene="bioD"
                     /locus_tag="VHM65_02645"
     CDS             complement(826..1539)
                     /gene="bioD"
                     /locus_tag="VHM65_02645"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX2704632.1"
                     /translation="MAEPISLPRVVVVTGTSTDVGKTVVTAALAALVLARGSTVAVVK
                     ATQTGVGPGDPGDVDVIRALLGADPHLQTHELLRLRAPLAPDAAARQEGVQIPTVADY
                     TRRVAELVSTADVVLAEGAGGLLVRLDQRGGTLADLAVALRDKGVSTGVVVVVAPGLG
                     SLNHTALTAEALRTRGIPLLGLVIGSWPSDPGLAERSNLEDLPSVAGAPLIGRVPAGA
                     GALDAAAFRYQSPGWFTVH"
     gene            complement(1532..2662)
                     /locus_tag="VHM65_02650"
     CDS             complement(1532..2662)
                     /locus_tag="VHM65_02650"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEX2704633.1"
                     /translation="MNEFLDWLSAAATRREELGLTRRLRAVKPGELLDLAGNDYLGLL
                     RHPAVVSGAVAAAQTYGGGAGASRLVTGTLPIHDELESRLASLTRSPAALVFSTGYHA
                     NLAAVTALTDAQTLLVSDAHIHASLVDAARLSRATVAVAGHCDVDAVVRLLRGRDRHT
                     HPRALVMVESIYSVLGDAAPLAALAEVCMALDAVLLVDEAHGIGVAGPAGEGLAAQLG
                     LTAEPGVVTTVTLSKALAAQGGAVLGHPAVRDHLVNTARPFIYDTGLAPGAAGAALGA
                     LDVLAAEPHRLGAIRRNAELLADACGVARPDGAVLSVAMAGPAEALAAVADAEARGIR
                     MGCFRPPSTPDKTSRLRLTAHANHEPEDLAPALDLLRGLLRG"
BASE COUNT          404 a         1008 c         1061 g          356 t
ORIGIN      
        1 cctggccaag gaccccgatg ccgggctgct cggctaccac gaatggctgg ggcgcaggac
       61 tctcctgctg tcctactggc gcagcccgga gcacctcatg cggttcgccg cgaaccccga
      121 tggcccccac gttggcccgt ggcgtgactt catgcgccgc gccgggtcga acccggcggt
      181 cgggatctgg cacgagacct atcggatccc ggtcggccac agcgaggcga tctacgccaa
      241 catgcccgag ttcggcctgg ccaaggccac cacacgcgtc ccgatcgtcc cgggcacgca
      301 gaccgccaga gccaggttca gcttccgggc accaccgtcg agcgcaggtg cgtgagcaga
      361 ccgcggcagg cggcctcgac ctcctgcagg caccgctcgt agtcggccgc gtccccgtag
      421 taggggtcgt cgacctcgag ggttccggcc gcgacggcac tggggtcgaa ctcccgcatc
      481 aggcgcaggg ccgcccggtg cccgggggta cgggccagcc gctgcagctg gcggacgtga
      541 ccctcgtcgg cggccaggac caggtcgtgg gtctcgaaga cgtccgaggt gacctgccgc
      601 gcccggtggg ctgacccgtc atacccatgg tggcgcaacg cggtaagggt gggccggtgc
      661 gcgggctgac cctcgtgcca gccacccgtg ccgaacgagt cgacctcgac gaggcccgcc
      721 atcccggctc cctgggcaag gcggcgcagg atgacctcac ccatcggcga gcggcagatg
      781 ttgccactgc acacgaacgt cacgcgaaag acctctgcca tggccctagt gaaccgtgaa
      841 ccagccgggc gactggtatc ggaacgccgc agcgtcaagc gcccctgccc ctgccgggac
      901 ccggccgatg aggggcgcgc ccgccacgga cggcaggtcc tcgaggttgg agcgctccgc
      961 gaggccgggg tcgctcggcc aggagccgat gacgagcccg agcagcggga tccccctggt
     1021 gcgcagggct tctgccgtca gcgcggtgtg gttgaggctg cccagaccgg gggccacgac
     1081 gacgacgaca cccgtgctga ctcccttgtc gcgcaacgcg acggcgaggt cggcgagcgt
     1141 tccgccgcgc tggtcgagcc gtacgagcag ccctccggcg ccctcggcga ggacgacatc
     1201 cgcggtggac accagctcgg ccacccgacg ggtgtagtcg gcgacggtgg ggatctgaac
     1261 cccctcctgg cgggctgcgg catcgggtgc gaggggtgcc cgcagccgga gcagctcgtg
     1321 ggtctgcagg tgtggatcgg ccccgagcag ggcgcgtatg acgtccacgt cacctgggtc
     1381 gcccggcccg accccggtct gggtcgcctt gacgacggca accgtggacc cgcgggccag
     1441 gacgagggcg gcgagcgccg ccgtcacgac ggtcttgccg acgtcggtgc tcgtgcccgt
     1501 gacgacgacg acgcggggca gcgagatcgg ctcagccacg caggagccca cggagcaggt
     1561 ccagagcggg tgccaggtcc tcgggttcgt ggttggcatg ggcggtcagc cgcagtcgcg
     1621 aggtcttgtc aggtgtcgac gggggccgga aacagcccat ccggatgcct cgcgcctcgg
     1681 cgtccgccac ggcggccagg gcctcggccg gacctgccat ggcgaccgac aggactgcgc
     1741 cgtcgggccg tgccacgccg caggcgtcgg cgagcagctc ggcgttgcga cggatcgcgc
     1801 cgagccggtg gggttcggcg gcgaggacgt cgagggcgcc gagcgctgcg ccggcggcgc
     1861 ccggggccag gccggtgtcg tagatgaacg ggcgcgccgt gttgacgagg tggtcccgca
     1921 ccgcgggatg accgagcacc gccccgcctt gtgccgcaag ggccttggac agcgtcacgg
     1981 tggtcacgac accgggctcg gcggtcaggc cgagctgggc cgcgagccct tcgccggccg
     2041 gcccggccac gccgatgccg tgggcctcgt cgacgagcag caccgcgtcg agggccatgc
     2101 acacctcggc cagcgcggcc agcggcgccg cgtcgccgag caccgagtag atcgactcga
     2161 ccatgacgag ggcccgtggg tgcgtgtgcc ggtcgcgccc gcgcaggagg cgaacgacgg
     2221 catcgacgtc gcagtggccg gcgacggcca ccgtggcccg gctcaggcgg gcggcatcga
     2281 cgagcgacgc gtggatatgg gcgtcggaga cgaggagcgt ctgggcgtcc gtcagcgcgg
     2341 tcacggcagc gaggttggcg tggtagccag tcgagaacac cagcgcagca ggggatctcg
     2401 tcaaggaggc cagccgggac tcgagctcgt catggatcgg cagcgtgccg gtgaccaggc
     2461 gggacgcgcc cgctccgccg ccatacgtct gggcggctgc cacggctccg ctcacgaccg
     2521 ccggatggcg cagcagcccg aggtagtcgt tgccggcgag gtcgagcagc tcgcccggct
     2581 tgacggctcg cagtcggcgc gtcaggccga gctcctcgcg gcgcgtcgcg gcggcactga
     2641 gccagtcgag gaactcattc acacgccgat cctgccacgg ggaggcacac cggcgccggg
     2701 ggaggacgcc ggctcacgcc tgccaccggg accacgccgc ctgtttggtc gtcccggagt
     2761 agtcgccgac ctccgacaac gacctcgtcg gccgcgagcg tgcgtgccga ctctccgagc
     2821 gcctcgccg
//
LOCUS       DATAIX010000176         2896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_159131, whole genome shotgun sequence.
ACCESSION   DATAIX010000176 DATAIX010000000
VERSION     DATAIX010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159131"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="VHM65_02655"
     CDS             complement(<1..1315)
                     /locus_tag="VHM65_02655"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX2704634.1"
                     /translation="MPGTTTPRSVALVTLGCTRNEVDSEELAGRLQAAGWALVEDAAD
                     ADVAVVNTCGFIEAAKKDSIDALLEASELKRSGRTQAVVAVGCLAERYGVQLAEELPE
                     ADAVLGFDSYAGLSGHLTAILGGAKVASHPPRDRRTLLPLAPATRAASAGGVALPGHG
                     TTAAPDPQTRDAPYPGSGPRVIRARLDGRPWAPLKIASGCDRRCGFCAIPMFRGSFVS
                     RQPADVVAEGRWLAGEGVRELFLVSENSTSYGKDLGDLTLLEKLLPELAAIDGIERVR
                     VSYLQPAEVRPSLLDAIAGTPGVVPYFDLSFQHASGPLLRRMRRFGDGEAFLHLIGQV
                     RDRVPAAGIRSNVIVGFPGESEADVEALTSFLERARLDVIGAFGYSDEDGTEAFGLDG
                     HLDGDVIADRLDAVTTLLEELTAQRAEERVGEVVRVLVEEVDAETG"
     gene            1556..2755
                     /locus_tag="VHM65_02660"
     CDS             1556..2755
                     /locus_tag="VHM65_02660"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX2704635.1"
                     /translation="MTSDALVLRSITDLLQAHPLIDGHNDLPWEAREQVRYDFDRLDL
                     AQPVATTCTDIPRLRAGCVGGQFWSVFVPSTRQGDDAVVTTLEQVDAVHQMIGRYGDA
                     LGLARTAGEVERVFASGRVASLLGAEGGHSIGCSLGALRMLHVLGVRYLTLTHNDNVP
                     WADSATDTPVVGGLSRFGVEVVREMNRLGMMVDLSHVSADTMRAALDASVAPVIFSHS
                     SARALCDVPRNVPDDVLGRLGANDGLCMVTFVPEFISPVVAAWRDEAAAAGTALGLNP
                     YDWDTFMPFALEWQNQSPKPEATLGEVVSHVEHVRDVAGIEHVGIGGDYDGTDTFPVG
                     LRDVSGYPALFEALRHRAWSDADLVALAGGNVLRVMRGVEDVAARLQQSRGPSLATFA
                     ELDTPTR"
BASE COUNT          390 a         1044 c         1046 g          416 t
ORIGIN      
        1 cgcccgtctc ggcgtcgacc tcctcgacga ggacccggac gacctcgccg acgcgctcct
       61 cggcccgctg ggcggtgagc tcctcgagca gggtcgtcac ggcgtcgagc cggtcggcga
      121 tgacgtcccc gtccaggtgc ccgtcgaggc cgaaagcctc cgtgccgtcc tcgtcggagt
      181 agccgaacgc cccgatcacg tcgagccggg cccgctcgag gaagctcgtc agcgcctcga
      241 cgtcggcctc gctctccccg gggaagccga cgatgacgtt ggaccggatc ccggccgccg
      301 gcacccggtc gcggacctgc cctatgaggt ggaggaacgc ctcgccgtcc ccgaaccggc
      361 gcatccggcg caacagcggc ccggacgcgt gctggaagga caggtcgaag tagggcacca
      421 cgccgggcgt ccccgcgatc gcgtcgagca ggctggggcg cacctcggcc ggctgcaggt
      481 aggacacccg cacccgctcg atcccgtcga tggcggcgag ctcgggcagg agcttctcga
      541 gcagggtgag gtcgccgagg tccttgccgt aggacgtgga gttctcgctg acgaggaaga
      601 gctcgcgcac gccctccccg gcgagccagc gcccctcggc gacgacgtcg gccggctgcc
      661 gggagacgaa cgacccgcgg aacatcggga tcgcgcagaa cccgcaccgc cggtcgcagc
      721 cgctcgcgat cttcagcggc gcccagggtc gcccgtccag gcgcgcgcgg atcactcgcg
      781 gaccagagcc cgggtatggc gcgtcccggg tctgcgggtc gggcgcggct gtggtgccgt
      841 gacccggcag ggccaccccg ccggcgctcg cggcgcgggt ggccggagcg agcggaagca
      901 gggtgcgccg gtcgcgcggg gggtgcgagg cgaccttcgc gccgccgagg atcgcggtca
      961 ggtgcccgga caggccggcg tacgagtcga acccgagcac cgcatcggcc tcgggcagct
     1021 cctcggcgag ctgcacgcca taccgctcgg cgaggcagcc gacggccacg acggcctgcg
     1081 tgcgcccgct gcgcttgagc tcggaggctt cgagcagggc gtcgatcgag tccttcttgg
     1141 cggcctcgat gaagccgcag gtgttgacga cggccacgtc ggcgtcggcg gcgtcctcga
     1201 cgagggccca gcccgctgcc tgcaggcgcc ccgccagctc ctcggagtcg acctcgttgc
     1261 gggtgcagcc cagggtcacc agggcaacgc tgcgaggcgt cgtggtcccg ggcatgacgc
     1321 cactctagac ggggcacggg cgcacccgga cgccagacct catccgcccc gcgctcgtcc
     1381 ggtgccgcga tcggcgaaag cccccggctg cggaagggcc atagcagggt gatggcccgc
     1441 acctaggcgg cgccgtcgac gcgccgggtg gaggaggatt gttggacgtg gccggggtgc
     1501 tgcccacagc ggcttggtaa agggagtggg tcgacgactg gctatcgtga ggcgcatgac
     1561 gtccgacgcc cttgtgctgc gctcgatcac cgacctgctg caggcgcatc cgctcatcga
     1621 cgggcacaac gacctcccct gggaggcgcg tgagcaggtc cgctacgact tcgaccggct
     1681 cgacctcgca cagcccgtcg ccacgacgtg caccgacatc ccgcggctgc gcgccgggtg
     1741 cgtcggcggg cagttctggt cggtcttcgt cccctcgacg cgccagggcg atgacgccgt
     1801 ggtcaccacc ctggagcagg tcgacgccgt ccaccagatg atcggccggt atggcgatgc
     1861 gctgggtctg gcacgcacgg ccggtgaggt ggagcgcgtc ttcgcctccg gccgggtcgc
     1921 ctcgctgctc ggtgccgagg gcgggcactc gatcgggtgc tcgctcggtg ccctgcggat
     1981 gctgcacgtc ctgggggtcc ggtatctgac gctcacgcac aacgacaacg tgccgtgggc
     2041 cgactccgcg acggataccc ctgtcgtcgg cgggctttcg cgtttcggtg tcgaggtggt
     2101 gcgcgagatg aaccggctcg ggatgatggt cgacctctcg cacgtgtcgg ccgacacgat
     2161 gagggccgcc ctggatgcca gcgtcgcgcc ggtcatcttc agccactcct cggcccgggc
     2221 cttgtgcgac gtcccccgca acgtccctga cgacgtcctg gggcgcctcg gcgcgaacga
     2281 cggcctgtgc atggtgacct tcgtgccgga gttcatctcc cccgtcgtcg cggcctggcg
     2341 cgacgaagcg gccgcggcgg ggactgcgct ggggctcaac ccctacgact gggacacctt
     2401 catgccgttc gcgctggagt ggcagaacca atcgcccaag cccgaggcca cgctcggcga
     2461 ggtcgtctcg cacgtcgagc acgtccgcga cgtggccggg atcgagcacg tcggcatcgg
     2521 cggtgactac gacgggaccg ataccttccc cgtcggcctg agggacgtca gcggctaccc
     2581 ggcgcttttc gaggccctgc gccaccgcgc ctggtccgac gccgacctcg tcgccctggc
     2641 cggcggcaac gtgctacgcg tcatgcgtgg tgtcgaggat gtcgccgcac gtctgcagca
     2701 gtcccgcgga ccgagcctgg cgaccttcgc cgagctcgac accccgacgc gctaacggtc
     2761 acggtcggag gtgccgccca tcgtcgcggt tgggagggaa ggagtcctcc tcgtcgggcg
     2821 cgcagcccat gagcctttag cgccgccata ctcgcgacgg ggatctctcc cggctcttgc
     2881 atgatgagcc ggatcg
//
LOCUS       DATAIX010000177         6069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_99273, whole genome shotgun sequence.
ACCESSION   DATAIX010000177 DATAIX010000000
VERSION     DATAIX010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99273"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VHM65_02665"
     CDS             complement(<1..407)
                     /locus_tag="VHM65_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HEX2704636.1"
                     /translation="MASYDFARPDRFVAGTVGPPGQRTFFLQAREGRALVSASLEKQQ
                     VGLLADRVNDLLDDFAGAAGTEEAAAGAVDNDPLDTPIEDEFRVVTIALSWDPDGQRV
                     VIECDDEPPGADDEVEGDAVTREGGDDLLAPHDT"
     gene            complement(418..1185)
                     /locus_tag="VHM65_02670"
     CDS             complement(418..1185)
                     /locus_tag="VHM65_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HEX2704637.1"
                     /translation="MPIVLLVRHGRTAANASGLLAGWTAGVELDAEGVRQAEGTGARI
                     AALGHPIARVVSSPLRRCVQTAAVIAAACGPSPPGPGVETDERLGECRYGAWTGRALA
                     ELAGEDLWRVVQDQPSAARFPDGADFPGESIAAMQARALDVIRSVDVEVAAAHGPHAL
                     WVAVSHGDVIKSVLADAAGAHLDAFQRMHVDPASVSVVRYTARRPFVLKVNHTGGDLL
                     GIIPAPHPAPSPDAAGEAVGDLDGDAAVGGGAGPAPG"
     gene            1289..2263
                     /locus_tag="VHM65_02675"
     CDS             1289..2263
                     /locus_tag="VHM65_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HEX2704638.1"
                     /translation="MIIDQAIYRHGRREACEDLSDAVTMLREEDEGFVWVGLKDPSDA
                     EFALVNTELDLHPLAVEDAVSGNQRPKADIYDETMLVVLKTLRYIEASSDIETGEVMV
                     FVGDRFVVTVRRGEGNPLAGVRLRLEANPEQLELGPGAVLHAITDSVVDNYIAIDIEL
                     ARDLDAIEEEVFSGTLEVDPAEIYRLKREVLEYRRAAEPLLEAMRTFHGHKVRELIHP
                     DCLPFFRDVEDHLHRVNDHARTYDRLLTDVLHAHLAQLQVQQNEDMRKISAMVALAAV
                     PTMIAGIYGMNFAHMPELEESWGYPAVLLLMLGACGFIYHAFRKANWL"
     gene            complement(2418..3272)
                     /locus_tag="VHM65_02680"
     CDS             complement(2418..3272)
                     /locus_tag="VHM65_02680"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001392.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX2704639.1"
                     /translation="MADLTYPASIVLGIVEGLTEFLPVSSTGHLTVAEELLGLQVDAP
                     EVTAYTAIIQLGAIAATFLYFAGDILRLFKAWVGGLRNPVRRADPDYGLAWAVLVGSL
                     PVALVGFALKDLIEGGLRSLWVVAAALILWSAVMVVAERRHVVLERQGVTRGEHDVRM
                     RDGLVIGLVQCFSLIPGVSRSGATISAGLVRGIDRVTATRLSFFMAIPALTAAGLYEG
                     AKTDLSGLGLGPLVLGIVIAFVVAYASIAWLLRFVAGHTLLSFVWYRVAAGLVLILAL
                     GAGWVSAT"
     gene            complement(3338..4396)
                     /locus_tag="VHM65_02685"
     CDS             complement(3338..4396)
                     /locus_tag="VHM65_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX2704640.1"
                     /translation="MELGLNLGNWGSGTDAGNIVLAQEADRLGYSVVWVAEAYGSDAP
                     TVLSWVGAKTTRIGLGSAVMQIPARTPAMTAMTAATLDTLSGGRFRLGLGVSGPQVSE
                     GWHGVRFASPLARTREYVEIVRMALRRETVAYDGAHWTLPLPDGPGKALKLSVHPVRS
                     AIPVYLAAVGPRNLELAGEIADGWLAIFFSPEHSREQLDNLRAGARRAGKGTAEDPLA
                     GFDVVPTVPVVIDDDVERAAAALRPYCALYLGGMGSREKNFYNQLAVRMGFADAAAQI
                     QELYLAKRHRDAAAAVPLEFIDATALLGPRERIKGRISRYADAGVTTLSISPGAPTLE
                     GRLVAVRLMAEVMGETGL"
     gene            4417..5358
                     /locus_tag="VHM65_02690"
     CDS             4417..5358
                     /locus_tag="VHM65_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2704641.1"
                     /translation="MRRRRLGSSGLAVSRLGLGTMLWGGLVGEEVARDQLSLFVERGG
                     TLVDTAHGYGAGLAEQILGRLLGDVVAREDVVVCTKAGVESRASEKVVDASRGTLLRQ
                     LDQSLRRLGTDYVDLWLVHAYDPSVPLAETMFTLEWAVTSGKARYVGVSNYSGWQAAR
                     AFSLLERRAGAPLVANQVEYSLVRRHAEDEQVPAALALGMGVLAWAPLGRGVLTGKYR
                     HTVPAKSRGASASFPGFADRYLDPHSVAVTEAVMTAAQGMAVPAAAVALAWLRDRPGV
                     TAPVVGARTLAQLKIAVDSEAVDVPTEIADALDDVSE"
     gene            complement(5348..5842)
                     /locus_tag="VHM65_02695"
     CDS             complement(5348..5842)
                     /locus_tag="VHM65_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704642.1"
                     /translation="MTPDPRAALAVLVSALERHLEAAASRRDPDDPTVTAAAQDLAEA
                     FDAYDETLFDATEVATPLAIYDGPFEDDDSDDEDDEDEDEDDDDEDDEDEEDEEDEDL
                     DDDDEDTGIYSGLEDVDLDDDSDDTDSDDEDDDEDDDDEDGEDADSDDEDGPDDEEPA
                     GVIR"
     gene            5881..6066
                     /locus_tag="VHM65_02700"
     CDS             5881..6066
                     /locus_tag="VHM65_02700"
                     /inference="COORDINATES: protein motif:HMM:NF040150.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 family protein"
                     /protein_id="HEX2704643.1"
                     /translation="MRDYEYRVLTFVPGVSRSDLRRELTELAEYGHWELARTVVYAGG
                     WRRVWLRRRVIRVLRTA"
BASE COUNT          871 a         2135 c         2186 g          877 t
ORIGIN      
        1 gtgtcgtggg gggcgagcag gtcgtcgccg ccttcccggg tcaccgcgtc accctcgacc
       61 tcgtcgtccg cgcccggggg ttcgtcgtcg cactcgatga cgacccgctg gccgtccggg
      121 tcccacgaca aggcgatcgt cacgacgcgg aactcgtcct cgatcggggt gtcgagcggg
      181 tcgttgtcga cggcgccagc cgcggcctcc tcggtgccgg cggcaccggc gaagtcgtcg
      241 agcaggtcgt tgacccggtc ggcgagcaga ccgacctgct gcttctccag ggatgccgac
      301 accagggcgc ggccctcgcg ggcctggagg aagaaggtcc gctgcccggg aggaccgacc
      361 gtccccgcga cgaaacgatc ggggcgcgcg aagtcatagg acgccatacc gaagacccta
      421 gcccggggcg ggtcccgcgc cgccaccgac cgccgcgtca ccgtcgaggt cgcccaccgc
      481 ctcacccgcc gcgtccggtg acggggcggg atgcggcgcc gggatgatcc cgagcaggtc
      541 gccgccggtg tggttgacct tgagcacgaa cggacgccgc gcggtgtagc gcacgacgct
      601 gaccgaggcc gggtcgacgt gcatgcgctg gaacgcgtcg agatgcgctc ccgcggcgtc
      661 ggcgaggacc gacttgatga catcaccatg ggagacggcg acccacagcg cgtgcggccc
      721 gtgcgcggcg gcaacctcca cgtcgacgga ccgtatgacg tcgagcgccc gagcctgcat
      781 cgccgcgatc gactcgcccg ggaagtccgc gccgtcgggg aagcgagccg cgctgggctg
      841 gtcctggacg acccgccaga ggtcctcccc cgcgagctcg gccagggcgc ggccggtcca
      901 cgcgccatac cggcactcgc cgagccgctc gtcggtctcg acgccgggac ccggcggact
      961 gggcccgcag gcggccgcga tgaccgccgc ggtctggacg cagcgccgca gcggtgagct
     1021 gacgacccgg gcgatcgggt gccccagcgc ggcgatccga gcaccggtgc cctcggcctg
     1081 gcgtacccct tcggcgtcga gctcgacgcc cgccgtccac cccgcgagca ggccggaggc
     1141 gttcgcggcc gtccggccat ggcggacgag caggacgatg ggcacggcga cactctagag
     1201 ggctcgcccc ttcggccggt cccgggcata ccatggtccg cggcgcccgg gcgttcccgc
     1261 gctgcgcgcc ctcgagagga gtcggcccgt gatcatcgac caggcgatct atcgacacgg
     1321 ccgacgcgaa gcctgcgagg acctcagcga cgcggtgacc atgctgcgcg aggaggacga
     1381 aggcttcgtc tgggtcggtc tcaaggaccc gagcgacgcc gagttcgccc tcgtcaacac
     1441 cgagctcgac ctgcacccgc tcgcagtcga ggatgcggtg agcggcaacc agcggcccaa
     1501 ggccgacatc tacgacgaga cgatgctcgt cgtgctcaag acgctgcgct acatcgaggc
     1561 gagctcggac atcgagaccg gtgaggtcat ggtgttcgtc ggtgaccggt tcgtcgtcac
     1621 cgtccgccgg ggcgagggca acccgctcgc cggagtgcgg ctgcggctcg aggcgaaccc
     1681 cgagcagctc gagctcggcc ccggcgccgt cctgcacgcg atcaccgact cggtcgtcga
     1741 caactacatc gcgatcgaca tcgagctggc gcgtgacctc gacgcgatcg aggaggaggt
     1801 cttctccggg accctcgagg tcgaccccgc cgagatctac cggctcaagc gggaggtcct
     1861 ggagtaccgc cgggcggccg agccgctgct cgaggcgatg cggaccttcc acgggcacaa
     1921 ggtccgcgag ctgatccacc ccgactgcct gccgttcttc cgcgacgtcg aggaccacct
     1981 gcaccgggtc aacgaccacg cccggaccta tgaccggctg ctcaccgacg tcctgcacgc
     2041 ccacctggcc cagctgcagg tgcagcagaa cgaggacatg cgcaagatct cggcaatggt
     2101 cgccctcgcg gcagttccga ccatgatcgc ggggatctac gggatgaact tcgcccacat
     2161 gcccgagctg gaggagtcgt ggggctatcc cgcggtgctg ctcctcatgc tcggcgcctg
     2221 cggcttcatc taccacgcgt tccgcaaggc gaactggctt tagcgccagt cgcattgggc
     2281 cggcacaact ccctgggtgt cgccgcccgt gtcggcggac cggccaactg gccgaacccc
     2341 gtcctggtgc ccgttgtgcc ccggtatatc cccctgctgg cgggattagg ccagttcggg
     2401 tggccggggg ctgcgggcta ggtcgcgctg acccagccgg cgccgagggc caggatgagg
     2461 acgaggccgg ccgccacgcg gtaccacacg aaggacagca gcgtgtggcc ggccacgaag
     2521 cgcagcagcc aagcgatcga ggcgtaggcc acgacgaacg cgatgacgat gccgagcacg
     2581 agcggcccca aacctagacc gctgaggtcg gtcttcgcgc cctcgtagag accggctgcc
     2641 gtcaaggccg ggatggccat gaagaaggac agccgggtcg cggtgacccg gtcgatcccg
     2701 cggaccagcc cggccgagat cgtcgcaccc gagcgggaga ccccggggat cagcgagaag
     2761 cactggacca ggccgatgac gagcccgtca cgcatcctca cgtcgtgctc gccgcgggtc
     2821 acaccctggc gctccagcac cacgtggcgc cgctcggcga cgaccatgac cgcgctccag
     2881 aggatgagcg ccgccgcgac gacccagagc gagcgaaggc cgccctcgat gaggtccttg
     2941 agcgcgaagc cgaccagggc gaccgggagc gaaccgacga ggacggccca ggcgaggccg
     3001 tagtcagggt cggcgcggcg gaccgggttg cgcaacccgc cgacccaggc tttgaacagc
     3061 cgcaggatgt cgccggcgaa gtagaggaac gttgcggcga tcgcgccgag ctggatgatc
     3121 gccgtgtatg ccgtcacctc cggcgcgtcg acctgcaggc cgagcagctc ctcggcgacg
     3181 gtgaggtgcc cggtcgagga cacgggcagg aactcggtga gcccctcgac gatgccgagc
     3241 acgatcgacg cgggataggt caggtctgcc acgggagagg ttccttcact cgttacggga
     3301 cggcgccggg agtgtcaccg ctcgtcgggc acccgcgtca gaggccagtc tcccccatga
     3361 cctcggccat caggcgcacc gccacgagcc ggccttcgag ggtgggcgcg cctggggaga
     3421 tgctgagcgt ggtgacgccc gcgtcggcat accggctgat ccggcccttg atgcgctcgc
     3481 gcggcccgag cagcgctgtg gcgtcgatga actcgagcgg cactgcggcc gcggcatcgc
     3541 gatgacgttt ggcgagatag agctcctgga tctgtgcggc cgcgtcggcg aagcccatcc
     3601 ggaccgccag ctggttgtag aagttcttct cccgcgaacc catcccgccg aggtagagcg
     3661 cgcagtaggg acgcagggcc gccgcggcgc gctcgacgtc gtcgtcgatg acgacgggca
     3721 cggtgggaac gacgtcgaag cccgcgagcg ggtcctcggc cgtgcccttg ccggcccggc
     3781 gcgcgcccgc gcgcaggttg tcgagctgtt cgcgcgagtg ctccgggctg aagaagatcg
     3841 cgagccagcc gtcggcgatc tccccggcga gctcgaggtt cctcggcccg accgcggcga
     3901 gatagaccgg gatcgcgctg cgcaccggat ggacgctcag cttgagggct ttgccgggac
     3961 cgtccgggag cggcagggtc cagtgggcgc cgtcatacgc aaccgtctcg cggcgcagcg
     4021 ccatccggac gatctcgaca tactcgcggg tgcgagcgag cggcgaggcg aaccgcacgc
     4081 catgccagcc ctcgctgacc tgaggaccgc tgacgccgag gccgagccgg aaccgcccgc
     4141 cggagagggt gtccagcgtc gcggcggtca tggccgtcat cgccggggtg cgggccggga
     4201 tctgcatgac cgcggagccg agcccgatcc gggtcgtctt ggcgcccacc caggacagga
     4261 ccgtcggggc gtccgagccg taggcctcgg caacccagac caccgagtag ccgagccggt
     4321 cggcctcctg ggccagcacg atgttgccgg cgtcggtgcc ggagccccag ttgcccaggt
     4381 tcagcccgag ttccatggcc agtaccgtat cgagtcatgc ggcgcagacg gctcgggtcg
     4441 agtggccttg ccgtgtcgcg cctcgggctc ggcacgatgc tgtggggagg cttggtcggc
     4501 gaggaggtcg cgcgcgacca gctgagcctg ttcgtcgagc gcggcggcac gctcgtcgac
     4561 acggcccatg ggtatggcgc ggggctggcc gagcagatcc tcggccgcct cctcggcgat
     4621 gtggtcgcgc gcgaggacgt ggtcgtgtgc accaaggccg gggtcgaaag ccgggccagt
     4681 gaaaaggtgg tcgacgcctc gcgcggcacg ttgctgcgtc agctcgacca gtcgctgcgg
     4741 cgcctcggca ccgactacgt cgacctgtgg ctcgtgcatg cctacgaccc gtccgtgccc
     4801 ctcgccgaga cgatgttcac cctggagtgg gcggtcacga gcggcaaggc ccgctatgtc
     4861 ggggtctcca actactcggg gtggcaggcg gcgcgggcgt tcagcctgct cgaacgccgt
     4921 gccggtgccc ccctcgtggc caaccaggtg gagtactcgc tcgtgcgccg ccatgccgag
     4981 gacgagcagg tgcccgcggc gctcgccctc gggatgggtg tgctcgcgtg ggcaccgctc
     5041 gggcggggtg tgctgaccgg caagtaccgt cataccgtcc cggccaagtc ccggggggcg
     5101 tcggcgtcgt tccccggctt cgccgatcgc tacctcgacc cgcactcggt cgccgtgacc
     5161 gaggcggtca tgactgcggc ccagggtatg gcggtccccg ccgctgcggt cgcgctggcc
     5221 tggctgcggg accggcccgg ggtgacggcc ccggtcgtgg gggcgcgcac gctggctcag
     5281 ctcaagatcg cggtggactc agaggctgtg gacgtgccga ccgagatcgc cgacgcgctg
     5341 gacgacgtca gcgaatgacg cctgcgggct cctcgtcgtc gggcccgtcc tcgtcgtccg
     5401 agtcggcgtc ctccccgtcc tcgtcatcgt catcctcgtc gtcgtcctcg tcgtcagagt
     5461 ccgtgtcgtc cgagtcgtca tcgaggtcga cgtcctcgag gcccgagtag atcccggtgt
     5521 cctcgtcgtc gtcgtcgagg tcctcgtctt cctcgtcctc ctcgtcctcg tcgtcctcgt
     5581 cgtcgtcgtc ctcgtcctcg tcctcgtcgt cctcgtcgtc cgagtcgtcg tcctcgaagg
     5641 gcccgtcgta gatcgcgagg ggcgtcgcga cctcggtcgc gtcgaagagt gtctcgtcgt
     5701 aggcgtcgaa cgcctcggcc aggtcctggg ccgctgccgt gacggtgggg tcgtcggggt
     5761 cccgccgcga cgcggctgct tcaaggtgcc gctcgagggc cgagaccagg acggcgagtg
     5821 cggcacgcgg gtccggggtc atgactcgac ggtatcggtt ttggcccaca atgggcagcg
     5881 atgcgtgact acgagtaccg agtcctgacc tttgtcccgg gcgtctcccg gtcggacctg
     5941 cgccgcgagc tcaccgagct cgccgagtac gggcactggg agctggcacg gaccgtcgtg
     6001 tatgccggtg gctggcgccg ggtctggctg cgccgacggg tcatccgggt cctgcgcacg
     6061 gcctgagcg
//
LOCUS       DATAIX010000178         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_99286, whole genome shotgun sequence.
ACCESSION   DATAIX010000178 DATAIX010000000
VERSION     DATAIX010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99286"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(857..1585)
                     /locus_tag="VHM65_02705"
     CDS             complement(857..1585)
                     /locus_tag="VHM65_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX2704644.1"
                     /translation="MPAARHRPGVGRVAGRRPLVDLRSLVEVVYTVHAPMDLRSILDS
                     AVPWHPLATCVETGAAVDLRPYLRQADDVQVAIRASAAMPVLAGGPIGLGGRHFYDGG
                     VAEPIPYRTAMGQGATHVLVLRSRRRDDIPKVSRTTPLFARAALRRYSHELERAYNHG
                     PVRHAEDDARLDGMTPNAAAGVALASIRPAPGSPRVGRLASDGGLLGEAFEAGRAAVH
                     EVFARMPGRTDATGCGPFALGSRT"
     gene            1543..>1783
                     /locus_tag="VHM65_02710"
     CDS             1543..>1783
                     /locus_tag="VHM65_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX2704645.1"
                     /translation="MRPYLRQADDVQLAIRASAAMPVLAGGPIRLGGRHFYDGGVSEP
                     IPYRTAMGQGATHVLVLRSRRRDDIPKVSRTTPLFA"
BASE COUNT          235 a          665 c          638 g          245 t
ORIGIN      
        1 agcaaggagc gccgagatga gcatgaaccc actcgacagc ggcaaggatg acgcggactc
       61 cggagccgac gctggcggcg acgccggagg cgacgccggc gcagacggag ggtcggacgg
      121 cggcgccagc gacccgaagt agtgcccgcg ttcgggcggc ccgtcatacc ccctcacgac
      181 cgggggtatg acggtctcgc ccgcgtgata ggcatcgacg agcagaagtt cgcccgggag
      241 ttctggggcc gtcgtccgac cctccgtctg cgccggcgtc gcctccggcg tcgcctccgg
      301 cgtcgcctcc ggtgtcggct ccggtgtcgg ccccagcgtc tgcgcccccg tcggctccgg
      361 cgtccgcgcc agcgtcggct ccggagtccg cgtcatcctt gccgctgtcg agtgggttca
      421 tgctcatctc ggcgctcctt gctcgtgggt caggtcaccg ggaggtgccc agtgcggctg
      481 acaatacccg cccggcccgc cgtcatacgt gctccggttg tcaccgacgg tggtggcacc
      541 cacgctcggg tgagcccagc cgccgctcga gggggtctca tggtcacacc gacacggtcg
      601 tccgctcacc gggcactcgc tggggtgttc gcggccacag tggtgccgct cgccgtcggc
      661 ctcatgctgc tccagagtgc ctcgggcgcg ccgaccgccc cccggcacac cgcgcgtcca
      721 ggagctcagc ccgagcacga ccgcgcagct ggtgcgggtc agggccccga caccgccgga
      781 tcgctcccga gtcgtggcgc tgggactcga tgtgaccgag gccgccggcg ccgacggtga
      841 caggcctgga cctgccctac gtcctggagc caagggcgaa cggcccgcag ccggtggcat
      901 cggtgcggcc gggcatgcgg gcgaacacct cgtgcacggc cgcacgaccg gcctcgaacg
      961 cctcccccag caggcctccg tcgctggcga ggcgtccgac gcgtggagag cccggcgccg
     1021 gcctgatcga ggccagcgcc acgccggccg ccgcgttggg cgtcatacca tccaggcgcg
     1081 cgtcgtcctc ggcgtggcgc accggcccgt ggttgtacgc gcgctcgagc tcgtggctgt
     1141 agcggcgcaa cgccgcgcgg gcgaagagcg gcgtcgtgcg cgacaccttg ggtatgtcgt
     1201 cgcggcgccg ggagcgcagg acgagcacgt gcgtggctcc ctgacccatc gcggtccggt
     1261 aggggatcgg ttcggccaca cccccgtcgt agaagtggcg gccgccgagc ccgatcgggc
     1321 cgccggcgag aacgggcatg gcggccgaag cccggattgc cacctgcacg tcatcggcct
     1381 ggcgtaggta gggtcgcagg tcgacggccg cgccggtctc cacgcacgtc gcgaggggat
     1441 gccagggcac ggcgctgtcc aggatcgacc gcagatccat cggggcgtgc accgtgtaga
     1501 ccacctcgac gagggacctc aggtcgacca gcggccgtcg acctgcgacc ctacctacgc
     1561 caggccgatg acgtgcagct ggcaatccgg gcctcggccg ccatgcccgt tctcgccggc
     1621 ggcccgatcc ggctcggtgg ccgccacttc tacgacgggg gtgtgtccga accgatcccc
     1681 taccggaccg cgatgggtca gggagccacg cacgtgctcg tcctgcgctc ccggcgccgc
     1741 gacgacatac ccaaggtgtc gcgcacgacg ccgctcttcg ccc
//
LOCUS       DATAIX010000179         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_9685, whole genome shotgun sequence.
ACCESSION   DATAIX010000179 DATAIX010000000
VERSION     DATAIX010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9685"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..958
                     /locus_tag="VHM65_02715"
     CDS             74..958
                     /locus_tag="VHM65_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEX2704646.1"
                     /translation="MTKKSGRKDRSAAKSDPPRTFAQALRVDQGFVLADLDPESTPGF
                     PGGKALGEKRFATRASGISELQERLFAESREGGRRSLLLVIQGMDTSGKGGLMRHVVG
                     QADPQGVRHTAFKAPSAEEREHDFLWRIRKALPVAGEIGVFDRSHYEDVLIVRVHDIV
                     PRAEWMTRYDLINAFEREVAASGTTIVKVMLHISKDEQKLRLAERLDRPDKYWKYNPG
                     DIAEREHWDDYMTAYQAALSTCSTLGAPWYVVPANRKWYARLAVQELLIEALEQMDPQ
                     WPAADFDIAVEKSRLAAS"
     gene            complement(1400..1525)
                     /locus_tag="VHM65_02720"
     CDS             complement(1400..1525)
                     /locus_tag="VHM65_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704647.1"
                     /translation="MSGTSTTAKASETTYKVKLSARVRTIPRTPLCPYMIEIVSR"
     gene            1903..1975
                     /locus_tag="VHM65_02725"
     tRNA            1903..1975
                     /locus_tag="VHM65_02725"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1936..1938,aa:Gly,seq:gcc)
     gene            1997..2070
                     /locus_tag="VHM65_02730"
     tRNA            1997..2070
                     /locus_tag="VHM65_02730"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2029..2031,aa:Cys,seq:gca)
BASE COUNT          285 a          726 c          725 g          348 t
ORIGIN      
        1 aggagtcgca tgacgcagta tggcgcgcac gtcaccgatg tcgtcggccc tccgatacgg
       61 caggatgtcg gccatgacga agaagtcggg gcgcaaggac cggtccgcgg ccaagagcga
      121 cccgccgcgg accttcgccc aggccctgcg ggtcgaccag ggcttcgtgc tcgccgacct
      181 ggacccggag tcgacgcccg gtttcccggg cggcaaggcc ctgggcgaga agcggttcgc
      241 cacccgggcc agcgggatca gcgagctgca ggagcggctg ttcgccgagt ccagggaagg
      301 cggacggcgc tccttgctgc tcgtcatcca gggcatggac acctcgggca agggcggcct
      361 gatgcgccat gtcgtcgggc aggccgaccc gcagggagtc cggcatacgg cgttcaaggc
      421 ccccagcgcc gaggagcgcg agcacgactt cctctggcgc atccgcaagg cgctgcccgt
      481 cgccggcgag atcggggtgt tcgaccggtc gcactacgag gacgtgctca tcgtgcgtgt
      541 ccacgacatc gtgccgcgcg cggagtggat gacgcgctat gacctcatca acgccttcga
      601 gcgcgaggtc gccgcgtcgg gcaccacgat cgtcaaggtc atgctgcaca tctccaagga
      661 cgagcagaag ctgcgcctcg ccgagcgcct cgaccggccg gacaagtact ggaagtacaa
      721 ccctggggac atcgccgagc gcgagcactg ggacgactac atgacggcct accaggccgc
      781 gctctccacg tgctcgaccc tcggggcgcc ctggtatgtc gtgcccgcca accgcaagtg
      841 gtacgcccgc ctcgccgtcc aggagctgct gatcgaggcg ctcgagcaga tggacccgca
      901 gtggccggcg gcggacttcg acatcgccgt cgagaagtcc cggctcgccg caagctgagc
      961 tgcggcgctg tcgtgggtgc tcacctcgtg gcgcggcggc gggtgccgtg atgctgcgcc
     1021 gtcgtctcgt cgcccgcctg gcccgggtgc ccgctgccgc gcccctggcc cggctcaccg
     1081 tcgagacgat ccgggtctgc ctgcgctacc gggtgaccgg gctcgcgtcg gaagcggcgt
     1141 tcttcatgct cctgtcgctg ccgccgctcc tgctcgggct gttcgcaggc gtcgggtatg
     1201 tcggtcagtg ggtcgggctc gcggcggtgt cagcggcggt gtgctcgatt cgggagtatt
     1261 ccggccagct cttcaccgcg cagagcgtca gcgacgtcat cgtgccgacg atcgacgagg
     1321 tcttccgggg cgggcgcgtc gagctggtgt cgctggggtt cctcctcgcc ctgtggtcgg
     1381 ggtcccgggc gctcaacgtc tatctcgaca cgatctcgat catgtacggg cagagcgggg
     1441 ttcgcgggat cgtgcgcacc cgcgcgctca gcttcacctt gtatgtcgtc tcgctggcct
     1501 tcgcggtcgt gctcgtcccg ctcatcctca tctatgggcc cctgaccgcc ccgatcgtcg
     1561 tgctcatctg gctctatgcc gtggccatct cgatcctcat cggtgctgcc ctgaactccg
     1621 ccgcccggag cctgtttcta actccggggg gtcctcgccc gggggtgcgg aggcctgtga
     1681 ggccatgccc gagcgatcgg tcacggcgcc gatctcgtcg ccgagcagtt cctccgccgg
     1741 gacgcgagcg cctcgcccgg ccagggccac gcacggccgc gagcgggccg ccatacccac
     1801 gccatacccg gcccggatgc cgcctgccgg tttggtcctc gcgggccgga tcggctaatg
     1861 tcggccaggc atccgcgggg tccgtgacct cgcgggtgat gtgcggacgt agctcagttg
     1921 gtagagcgca accttgccaa ggttgaggtc gcgagttcga gcctcgtcgt ccgctcggag
     1981 gatgtggcgc cttcacggtg gagtggccga gaggcgaggc agcggcctgc aaagccgcgt
     2041 acacgggttc aaatcccgtc tccacctcca accgctcaac cgag
//
LOCUS       DATAIX010000180         1883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_9776, whole genome shotgun sequence.
ACCESSION   DATAIX010000180 DATAIX010000000
VERSION     DATAIX010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9776"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(331..1478)
                     /locus_tag="VHM65_02735"
                     /pseudo
     CDS             complement(331..1478)
                     /locus_tag="VHM65_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611765.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          284 a          628 c          698 g          273 t
ORIGIN      
        1 ccagcaggca agctcgcggt ggcggcgatg gggccggcgg cccgcgccac gtggacggga
       61 ccggctcggc tgggccgact tcgcgatggg ctgccggggg ccgggcggcg ctcgggacgg
      121 gctgcgtgac gaccaccgac ccgggtgtgc accggatgag cgctgcgacc gccggcgatc
      181 gggcggtggt taagcctggg tcacggaatg cgccgcccga ccgccgggcg gtcattccgg
      241 ccacgaagtg cgcaatgcgg ccgtgaaccg ccctgacttc ggtggagact ctggggtgcc
      301 ctgagtttcg tggagtcgcg agactggatt tcacgcgacc gtctcggtct tgtgccgagt
      361 ctcgaactcg acaggggtaa gcatgccaag cgccgagtga cggcgctggc ggttgtggaa
      421 gatctcgagg tactcgaaga tcgcgttggc cagctcgacc ctggtccgcc aggtgcgccg
      481 gtcgaggagc tcgacctgca tccggctcca gaacgactcg atcatggcgt tgtcgaagca
      541 gctcccgacc gagcccatcg agggcatcag gcgagagtcg agcgctcggc gggtgaacgc
      601 ccaggaggtg aactgggtgc cctggtcgga gtggatgatc gtcccgtccg gttcgcggtt
      661 cgcgatcgcc atgcccaacg cgttggtgac gagggctgac gtggccgagg cgtcgatgga
      721 ccagccgacg accctccggc tgaaggcatc gagcacgacc gcgcagtaaa ccttgccctc
      781 gcgggtggga tgctcggtga tatcggtgac ccagagccgg tcgggatcgt cctgggcaaa
      841 gtgccgctcg acgaggtcgg acgcggttgg cacgtgcggg atgtgccgga agcgcggccg
      901 gccggacagc ccggccatcc ccgcccgagc catgaggagc tcgacggcgc agcggccgac
      961 ctcgaggcca tggcccaggg tgagctcggc gtggacgcgc cgggcgccgt aggtgccgcg
     1021 ggagtcgcgg tggatccgac cgatgagctc ggtcagatag gcgtgtcgca gggcccgcgc
     1081 cgacggaggg cggccgtgct gggcatagaa gccggactcg gagacggcca gcacccggca
     1141 cgcgagcagg acggagtgag cttccgcggc catcacctcg atcgccgcga accgcctttt
     1201 gggtggctgt gctccgccag cagctcactc gcccgccggt ggatcgcgag ctcggcttcg
     1261 agctcccgga tccggcgctt cgcggcggcg agctcctccc gctcgaccga tgtcagcccc
     1321 gcaaccaggc cctggtcgat ctgctcctgc cggcgccagg cgtagaccgt ctggctgctg
     1381 acgccgaggt cacgtgcgag gtcggcgacc ttgcgacccg cggtgaccag gtcgagcacg
     1441 cgtcgacgga actcgggcgg gtatccccgt cttcccatgg ctgcctccta ctgggggaca
     1501 gccgcatcat gtccagtaat cggactccac gaaaggcggg gcagaccaaa cccagggcgc
     1561 ttcagccgtg cggaactcac tcgccggtcg cagtgttcac tccgtggacg cgacgggcag
     1621 cggcggccgg ttgcgcgacc gctccaggcc agggtcgcgc cccggccgtc gtcgtgtcac
     1681 agagggttgg ggccgacagc ctggcccctg gtcgcgcatc gtcgccgagc acggcggggg
     1741 gcgcatccgc acgatatcct gtaggccatt ccacccaccc caggagcacc catgaaagtc
     1801 ggcgttccga gagaaaccct gcccggcgag cgccgggtgg cgctggtgcc ggaggccctc
     1861 ggcaagctca aggccgcggg cat
//
LOCUS       DATAIX010000181         1937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_272598, whole genome shotgun sequence.
ACCESSION   DATAIX010000181 DATAIX010000000
VERSION     DATAIX010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272598"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VHM65_02740"
     CDS             complement(<1..1127)
                     /locus_tag="VHM65_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX2704648.1"
                     /translation="MRSGTLVAGIDSSTQSCKVLVCRAENGQIVRQGRGVHPEGTEVD
                     PAHWWTAFEQATAGGLLDGVSAIGVGGQQHGMVTLAEDGRVVRPALLWNDTRSASAAT
                     DLVAELGGAQACAEAVGSVLVASFTVAKLRWLAEHEPGLAARVAHVVLPHDWLTHRLI
                     AAGDAASYTTDRGDASGTGYWSPVTGEYRLDVLARAFGRPIAVPRVLGPADVAGESAS
                     GMLVSAGTGDNMAAGLGLGLVPGDVAVSLGTSGTVFARHDAPTPDPSGFVAGFADATG
                     AFLPLVCTLNAARVLTATADMLGVDVAGLDRLALAGAPGAGGLTLLPYLDGERTPDLP
                     QATGTLGGITRANATPENLARSAVEGMLCGLADGLDALRAQG"
     gene            complement(1147..>1937)
                     /locus_tag="VHM65_02745"
     CDS             complement(1147..>1937)
                     /locus_tag="VHM65_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX2704649.1"
                     /translation="VVYHIAGCGQCEHCRRGYAVACSGPQRAAYGWQRDGGHAPYLLA
                     EEVSCLPLPDELSFADGALVSCGFGTAYEALLRAGAGGQDRLLVTGLGPVGLATAMLG
                     RALGVSDVIGSDVSASRREMALRLGLVGAAVGPDDLDDAVAARTAGRGCEVSVECSGN
                     GSARVSALRNTRSWGRCVFVGEGGDVTFAVSDLLIHRQITLYGSWVTSLLHMDQLLER
                     LARWRLHPEVIVTHRFGLDQADEAYRVAASGAGGKVCLVPNPAQ"
BASE COUNT          291 a          741 c          667 g          238 t
ORIGIN      
        1 ccctgagcgc gcagcgcatc gaggccgtcg gcaagcccgc agagcatgcc ttcgaccgcc
       61 gaccgggcga ggttctccgg ggtcgcgttg gcccgtgtga tccccccgag cgtccccgtg
      121 gcctgtggca ggtcgggcgt ccgctcacca tcgaggtagg gcagcagggt gagtccgccc
      181 gctcccggcg cgccggccag ggccagccgg tcgagaccgg cgacgtccac gccgagcatg
      241 tccgcggtgg cggtgaggac gcgggccgcg ttgagcgtgc acaccagagg caggaacgcc
      301 cctgtcgcgt cggcaaaccc agccacgaag ccgctggggt cgggcgtcgg cgcgtcatga
      361 cgggcgaaga ccgtccccga cgttcccagc gacacggcga cgtcgcccgg gacgagcccg
      421 aggccgagcc ccgctgccat gttgtcaccg gtgcccgccg atacgagcat cccgctggcg
      481 ctctcaccgg caacgtccgc aggacccagc accctgggga cggcgatcgg ccgtccgaat
      541 gcccgggcca ggacgtccag ccggtactcc cccgtcaccg gggaccagta gccagtcccc
      601 gacgcgtcgc cacggtcggt cgtgtatgac gcggcgtcgc cggccgcgat gagccgatgg
      661 gtcagccagt cgtgcggcag caccacgtgc gcgaccctcg cggcgaggcc gggttcgtgc
      721 tcggccagcc agcggagctt ggccacggtg aagctcgcga cgaggacgga tcccacggcc
      781 tcggcgcacg cctgagcgcc gccgagctca gccacgagat ccgttgccgc agacgccgac
      841 cgtgtgtcgt tccagagcag agccgggcgc acgacccggc cgtcctcggc cagcgtcacc
      901 atcccatgct gctgtccccc gacaccgatc gcgctgaccc cgtcgagcag cccgcccgct
      961 gtcgcctgct cgaaggcggt ccaccagtgt gccgggtcga cctcggtgcc ctcgggatgg
     1021 accccccgcc cctggcgcac gatctggccg ttctcggccc ggcagaccag caccttgcac
     1081 gactgggtcg aggagtcgat gccggcgacg agcgtgccac ttctcatgat ccgggtgtcc
     1141 ttcgcgtcac tgcgccgggt tgggtacgag acacaccttg ccacccgccc cggaggcggc
     1201 cacgcggtat gcctcgtcgg cctggtcgag cccgaagcgg tgcgtcacga tgacctcagg
     1261 gtgcagccgc catcgcgcca ggcgctccag gagctggtcc atgtggagca gcgacgtcac
     1321 ccacgaccca tacagggtga tctggcggtg gatgagcagg tccgagacgg cgaacgtcac
     1381 gtcgcctccc tccccgacga agacacaccg cccccacgaa cgggtgttgc gcagcgccga
     1441 gaccctcgcc gagccgttgc ccgaacactc cacgctgacc tcgcaaccgc gcccggccgt
     1501 gcgagccgca accgcgtcgt cgaggtcgtc agggcccacg gccgcaccga cgaggccgag
     1561 gcgcagcgcc atctcgcggc gcgaggccga gacgtcggac ccgatgacgt cgctgacacc
     1621 gagcgcgcgg ccgagcatgg ccgtggccag tccgaccggg ccgaggccgg tgacgagcag
     1681 gcggtcctgg ccgccggccc cggcgcgcag cagcgcctcg taagcagtgc cgaacccaca
     1741 cgacacgagt gccccgtcgg caaagctgag ctcgtccggc agtggcagac agctcacctc
     1801 ctcggccagg aggtatggcg cgtggccacc gtcccgctgc cacccgtagg cggcgcgctg
     1861 cggccccgag cacgcgacgg catacccccg tcggcagtgc tcgcactgcc cgcagccggc
     1921 gatgtggtag accacga
//
LOCUS       DATAIX010000182         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_242770, whole genome shotgun sequence.
ACCESSION   DATAIX010000182 DATAIX010000000
VERSION     DATAIX010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242770"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1085
                     /locus_tag="VHM65_02750"
     CDS             258..1085
                     /locus_tag="VHM65_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704650.1"
                     /translation="MSPEPQPFRALALLDLVTNARYGSGLGRRRLQRVMARPEREAVT
                     AVLERLPATVRAWSDDLATIEPLDIVEIRRPLSSLSSSGGGRWWAGPKDCREELLELA
                     AEHSYDSVLVLWPAAPELPLCGWGCTIGPSEATLGAGFTSIASDHWPTLATDPDPEQG
                     YVHEWLHQVESIYRELGVGEDELPGLHDAGDFTSTRPATEPPFGESYAVYHDGSPGRS
                     DGPGRSDEPGRPGARTWAPWYRDWMTGRLRRLADPGDDGSVPTPLGLTPERWARRTR"
     gene            1173..1775
                     /locus_tag="VHM65_02755"
     CDS             1173..1775
                     /locus_tag="VHM65_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2704651.1"
                     /translation="MATTVLTELRSARMDRGLGGADVARAAGISASQYSRIERGLTAG
                     LTIEQASILLATVGLELSVRAYPGGQPLRDAAHVALIDRLRTRVHRSLRFRTEVPFPS
                     PGDRRAWDVVIAGPNWRHGIEAETRPRDRQALERRLALKLLDGDVASLSLLLLDSRHN
                     RDFVRANQDVLAERFPVPGRRTLELLGAGVNPSGNSVLLL"
BASE COUNT          304 a          750 c          747 g          336 t
ORIGIN      
        1 cggctgctgg ccgctgcggc actagccgac cgctttcgcg cgctgggcgc ggacgggtcg
       61 accggcccgg tggtcacctc ttgcgggagt ggcgtctcgg cctgcttcaa cagcctggcc
      121 atgcgcgtcg ccggcctgcc agacccgatc ctctatccgg gttcgtacag tgactggagt
      181 cgggccggta agccggtggc agtcggcccg gagccgggcc ctgtgccagg gacgctgaga
      241 cggcgacgtc ctgggcggtg agccctgagc cgcagccgtt ccgagcgctc gccctgctcg
      301 acctggtcac gaacgcgagg tacggctcgg ggctgggtcg caggcgacta cagcgggtca
      361 tggctcgacc ggagcgagag gcggtgaccg ccgtcctgga gcgactgccg gcgaccgtcc
      421 gcgcctggag cgacgacttg gcgacgatcg agcccctcga catcgtcgag atccggcgcc
      481 ccctgtcgag cctgtcgtcg agcggtggcg gtcgctggtg ggcgggaccg aaggattgcc
      541 gcgaggagct gctcgagttg gccgccgagc attcgtatga ctctgtgctc gtcctgtggc
      601 cggcggcccc cgagctgccg ttgtgcggct ggggctgcac gatcggcccg tcggaggcca
      661 cgctcggggc cggattcacc tcgatcgcgt cggatcactg gccgaccctc gcgaccgatc
      721 ccgaccccga gcagggctac gtccacgagt ggctccacca ggtcgagtcg atctatcgcg
      781 agctcggcgt cggggaggac gagctgcccg gcctacacga cgccggcgac ttcacctcca
      841 ctcgcccggc gaccgagccg ccgttcggag agtcctacgc cgtgtaccac gacgggagtc
      901 cggggcgcag cgacgggccg gggcgcagcg acgagccggg gcggcccggc gcgcggacct
      961 gggcaccctg gtaccgcgac tggatgaccg gccgactccg ccgcctcgcg gatccgggcg
     1021 atgacggttc cgtccccacg cctctcgggc tcaccccaga gcgttgggct cgccgcacca
     1081 ggtaagcctc cggtgagcgg cgtcgtgcac catcccaagc catggggtct cgcgaacgag
     1141 ccgtcgacat cggtgcaagg cgtggccgag cgatggcgac cacggtgctc accgagctgc
     1201 ggtcagcacg gatggaccga ggtctcggcg gcgcggacgt ggcccgtgcg gcggggatct
     1261 cggcttcgca gtacagcagg atcgagagag gcttgacggc cggcctgacg atcgaacagg
     1321 cgtcgatcct gctcgccacg gtaggtctcg agctctccgt tcgtgcctat cctggcggcc
     1381 agccgctgcg tgacgccgcg cacgtcgccc tcattgaccg attgagaacc cgtgtccatc
     1441 ggtccctgcg attccggacc gaggtgccct ttccgtcgcc gggcgatcgt cgagcgtggg
     1501 acgtggtgat cgccgggccg aactggcgcc acggcatcga ggccgagacc aggccacgcg
     1561 accgtcaagc tctcgagcgt cgactcgccc tgaagttgct ggacggcgac gttgcgagcc
     1621 tgtcgctcct cctgctcgac tcgcgtcaca accgcgactt cgtcagggcc aaccaggacg
     1681 tcctcgccga gcgcttcccg gtcccaggtc ggcggacgct cgaattgctc ggtgcgggag
     1741 tcaacccgtc cgggaactca gtcctcctgc tctgagctcc aggggcgtct gaaccttgac
     1801 cccggtcgcc aacatgattg cgaccggggt cgcgggcacg attgcatcgg atgcaaccaa
     1861 cgtcgccggc gacgatcttc tccctcgatt cgtgcgggag aattgcatca gacgaaacgc
     1921 cagcgaccga ctgcgccgac cgcggcctcc agcccgctcg gttcatgcgt ttgacgcaag
     1981 ttgatggatg ggccgggccc ccgtccgaca gaacatgcat ctagcgcaac gtcctgccga
     2041 atccttgcgt ccagcgcaat ttgcgggccg cggtggcctt cgcccggccg agacaacccc
     2101 gaccaggccg gggagtcccg gcctggtcgg ggttgtc
//
LOCUS       DATAIX010000183         1759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_183368, whole genome shotgun sequence.
ACCESSION   DATAIX010000183 DATAIX010000000
VERSION     DATAIX010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183368"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..866)
                     /locus_tag="VHM65_02760"
     CDS             complement(228..866)
                     /locus_tag="VHM65_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX2704652.1"
                     /translation="MSAAMVGFLGLCLVMTLTPGLDTAMVMRNALRRGPVAGVLTAVG
                     CALGLFVHAAAVAVGLAAILLRSATIFEVVRWIGAVVLVVIGSLSLRAAITGRGVSAP
                     DLDAEIVRSSRRSSFAQGLVTNLTNPKATLFFLSALPQFVNPGSSTQALPQAMTLAVI
                     AATFSAVGLSVVGLLAGKARRWLSSVRTHRILEGVMGTVLVGLGIKVALDRP"
     gene            complement(1014..>1759)
                     /locus_tag="VHM65_02765"
     CDS             complement(1014..>1759)
                     /locus_tag="VHM65_02765"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX2704653.1"
                     /translation="CAEDILDLLRSDGPLPTTELPDTCAVPWRSSGWTHDKNVQKMLS
                     ALVARGEVALSRREGAMPWWDLAERVYPDDPPVSLADAARERNRRRLAALGIARAKAV
                     QTPVEPNDVGPVGVEALVEGVRGTWQVDPAQLERLAEPFEGRAALLSPLDRLVFDRKR
                     LAEIFDYDYALEMYKPAAQRIWGYWALPVLYGDRLIGKADAIAEREEGELIVHALYED
                     EPWTPGQRAAVVDELGSLARWLDLALVLP"
BASE COUNT          297 a          624 c          587 g          251 t
ORIGIN      
        1 gggtaagggc ggaacgcaac gcgatcgaga ccgcgtcacg gctcatggcg gtgagtttgg
       61 acgtgtccac cagtcatctc ctcgaaagtt gagcgtacta ggctcaaccc tactcgctga
      121 accgcccttt cgtgctgagc cgtccgacgc gcacaaccct tgtggtgcaa aaacgtggtc
      181 acacgggttg tgcgggcacc cgaaaggttg tgcggagggg cagcatctca ggggcggtcg
      241 agcgcgacct tgatgccgag gccgacgagg acggtcccca tcacgccctc caggatgcgg
      301 tgggtgcgca cgctcgacag ccagcgtcgc gccttcccgg ccaggagtcc gaccacgctc
      361 agcccgaccg cgctgaacgt cgcggcgatg acggccaacg tcatggcctg cgggagcgcc
      421 tgggtcgacg aacccgggtt cacgaactgc ggcagtgccg acaggaagaa gagggtcgcc
      481 ttggggttgg tgaggttcgt gacgagccct tgggcgaagg aggagcgtcg cgaggaccgt
      541 acgatctcgg cgtcgaggtc cggcgcagag accccgcgtc cggtgatcgc cgcgcgcaaa
      601 gacaacgagc cgatgaccac cagcaccacc gccccgatcc agcggacgac ctcgaagatc
      661 gtcgccgagc gcagcaggat cgctgccagc cccacagcga cagccgccgc gtgaacgaac
      721 agcccgagtg cgcatcccac ggctgtgagc actccggcca ccgggccacg ccgcaacgcg
      781 ttccgcatca ccatcgcggt gtcgagccca ggggtgaggg tcatcaccag gcacagtccc
      841 aggaacccca ccatcgccgc cgacatgcgt gaatcctgac aggccggcgg ctgccagacc
      901 acccctgatc cgtgcctggt ctgacgcacg cgaccgacat tcagtggcac ccacgcggtc
      961 gccggtcgcc atatgcgtcg gcggtgccct cagtgccact caatgtcgga gactcagggc
     1021 agcacgagcg ccaggtcgag ccagcgggca agggatccga gctcgtcgac gaccgccgcg
     1081 cgctgcccag gcgtccaggg ctcgtcctcg tacaacgcat gcacgatcag ctcgccctcc
     1141 tcgcgttcgg cgatggcgtc ggccttgccg atgagccgat ctccgtacag gacgggcaac
     1201 gcccagtagc cccagatgcg ctgcgcggcc ggcttgtaca tctccagcgc gtagtcgtag
     1261 tcgaagatct ccgccagccg cttgcggtcg aacaccagcc tgtcgagcgg cgacagcagc
     1321 gcggcgcggc cctcgaaggg ctcggccaga cgctcgagct gcgcggggtc gacctgccac
     1381 gtgcctcgta cgccctcgac gagcgcctcg acgcccaccg gcccgacatc gttgggctcg
     1441 accggcgtct gcaccgcttt cgcgcgtgcg atgccgagcg cggcgagtcg gcgacggttg
     1501 cgttcgcgcg cggcgtcggc gagcgatacc ggagggtcat cggggtagac ccgctccgcc
     1561 aggtcccacc agggcatcgc gccttctcgt cgcgacaatg ccacctcgcc ccgcgccacg
     1621 agcgcgctca gcatcttctg cacgttcttg tcgtgggtcc accccgacga tcgccagggc
     1681 accgcgcacg tgtcgggcag ctcggtggtg gggagcgggc cgtcgcttcg cagcaggtcg
     1741 agaatgtcct ccgcgcagg
//
LOCUS       DATAIX010000184         2417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_70110, whole genome shotgun sequence.
ACCESSION   DATAIX010000184 DATAIX010000000
VERSION     DATAIX010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70110"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..366
                     /locus_tag="VHM65_02770"
     CDS             52..366
                     /locus_tag="VHM65_02770"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2704654.1"
                     /translation="MTGSTALESSTPRESAAQQAALLSAVADPVRLGLVWRLAQGTRC
                     VCDLQTDPAIPGNLLSYHLRVLREAGLVTTSRRGRWVDYTLAHDALARLHAALPSGGE
                     PR"
     gene            363..1418
                     /locus_tag="VHM65_02775"
     CDS             363..1418
                     /locus_tag="VHM65_02775"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX2704655.1"
                     /translation="MTVTERRQRRGEYAVAARVAAAAVVWGAAYQVNEPLWTWLAGDV
                     MHLDLASRLGSGVLFFCYDTVKIALLLAGIIFAVTVLRSFMSVERTRALLGGKREGAG
                     NVIAACLGVVTPFCSCSAVPAFIGFVASGVPLGVTMSFLVASPLVNEIAIALLLGMFG
                     PGPTALYVAAGLVIAILSGFVIGRLRLERWVEPFVFETRLGGQLVDPSAALTWAQRVQ
                     MGTEEVASILRRIWPYLLVGIGLGAVIHGWAPEDFFAQYAGPGNPFGVLVAVLVGVPL
                     YSNAAGVLPLVEALYDKGLPMGTLLAFMMATVALSLPEMILLRRVLKPALIATFAAVV
                     SVGIIAVGYLFNAVLPS"
     gene            1447..1704
                     /locus_tag="VHM65_02780"
     CDS             1447..1704
                     /locus_tag="VHM65_02780"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX2704656.1"
                     /translation="MHVKILGPGCANCGTLEKNTHEALAALGLDPEVTKVTDYAEIAA
                     YGVMRTPALVVDDKVLLSGRVPKAAEIKDLLTQHGSAAAAR"
     gene            1737..2192
                     /locus_tag="VHM65_02785"
     CDS             1737..2192
                     /locus_tag="VHM65_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HEX2704657.1"
                     /translation="MSATPAVLFVCVKNGGKSQMAAALMRQVAGDLVEVPSAGPHPGT
                     AINALSAQVVAEVGADMSGEHPRPVDPRLLARVDRTIVLGAEAVVAPVPGMRGDIETW
                     ETDEPSARGIEGIVRMRLVRDDILTRVRALARELTSVGGSAGQGSPPPR"
BASE COUNT          310 a          847 c          851 g          409 t
ORIGIN      
        1 atctggaccg gcggtgaccc gtgccatgat tcaaatgtga ctagttcagc tgtgactggt
       61 tcaacagccc ttgagtcttc aactccccgt gagtcggcgg ctcagcaggc cgccctcctg
      121 tcggccgtgg ctgacccggt gcggctgggg ctcgtgtggc ggctggcgca ggggactcga
      181 tgcgtgtgtg acctgcagac cgaccctgcg atcccgggca acctgctcag ctaccacctg
      241 agggtcctgc gcgaggcggg cctggtgacg acgtcgcgcc ggggccggtg ggtggactac
      301 accctggcgc atgacgccct ggcccggctg cacgctgccc tcccctccgg gggtgagcca
      361 cgatgacggt caccgagcgg cgtcagcggc gcggggagta tgccgtcgcc gcgcgggtcg
      421 cggccgctgc cgtggtgtgg ggcgcggcct accaggtcaa cgagccgttg tggacgtggc
      481 tggccggcga cgtcatgcat ctggatctgg cctcccggct cgggtcgggg gtgctcttct
      541 tctgctatga cacggtcaag atcgcgctcc tgctcgccgg gatcatcttc gccgtcacgg
      601 tcctgcgctc gttcatgagc gtcgagcgga cccgggccct gctcggtggc aagcgagagg
      661 gcgccggcaa cgtcatcgcg gcatgcctcg gggtggtcac ccccttctgc tcgtgcagcg
      721 ccgtcccggc gttcatcggg ttcgtcgcct cgggcgtgcc cctgggggtg acgatgagct
      781 tcctcgtcgc cagcccgctg gtcaacgaga tcgcgatcgc gctgctcctc ggcatgttcg
      841 ggccgggtcc gaccgccctc tacgtggcgg cggggctggt catcgcgatt ctttccgggt
      901 tcgtcatcgg ccggctcagg ctggagcgct gggtcgagcc gttcgtcttc gagacccgcc
      961 tcggtgggca gctcgtcgac cccagcgccg cgctgacgtg ggcgcagcgg gtccagatgg
     1021 gcaccgagga ggtggcctcg atcctgcggc ggatctggcc gtacctgctc gtcggcatcg
     1081 gcctgggagc ggtgatccac gggtgggccc ccgaggactt cttcgcccag tacgcgggcc
     1141 cgggcaaccc gttcggggtc ctcgtcgcgg tcctcgtcgg ggtgccgctg tactccaacg
     1201 ccgccggggt cctgccgctg gtcgaggccc tgtatgacaa gggcctgccg atgggcaccc
     1261 tgctggcctt catgatggcc accgtcgccc tgagcctgcc cgagatgatc ctgctgcgcc
     1321 gggtcctcaa gcctgcgctg atcgccacct tcgcggctgt cgtctccgtc gggatcatcg
     1381 ccgtcggcta cctgttcaac gccgtcctgc cctcctgagc accacctcac gaaagcgaga
     1441 accgccatgc acgtcaagat cctgggaccc ggctgcgcca actgcggcac cctggagaag
     1501 aacacccatg aggcgctcgc cgccctcggc ctggaccccg aggtcaccaa ggtcaccgac
     1561 tacgccgaga tcgcggccta cggcgtcatg cgcaccccgg ccctggtcgt ggacgacaag
     1621 gtcctcctct ccggtcgagt ccccaaagcg gccgagatca aggacctgct cacccagcac
     1681 ggttccgccg cggcggcccg ctgactcgag tcacgccata ccgaagggag acccagatga
     1741 gtgccacgcc agcggtgttg ttcgtctgcg tgaagaacgg tggcaagtcc cagatggctg
     1801 ccgcgctgat gcgccaggtg gccggcgacc tggtcgaggt cccctcggcc gggccccacc
     1861 ccgggaccgc tatcaacgcg ctctccgccc aggtcgtcgc cgaggtgggc gcggacatga
     1921 gcggtgagca cccgaggccg gtcgacccgc gcctgctggc ccgggtggac cgcaccatcg
     1981 tgctgggcgc cgaggcggtc gtcgcgcccg ttcccggcat gcgcggggac atcgagacgt
     2041 gggagacgga cgagccgtcc gcgcggggca tcgaggggat cgtgcggatg cgcctcgtcc
     2101 gcgatgacat cctcacccgg gtccgtgcgc tggctcgcga gctcacctcg gtcggtggct
     2161 ctgcgggcca aggttcgccg ccgccgaggt gaggcgcgcc gtcatacgct gggcttcctg
     2221 gccggcacca aggaggaggc gcgatgtccc agacgttcga cgcgattgac gactcgatgg
     2281 cccggtggct tgccgcgcag ccgatgttct tcatcggcac ggcgccgctg gccgcgggcg
     2341 ggcacgtcaa cgtctcgccc aaggggatgg cggggacctt cgtcgtgctc ggggagcggc
     2401 aggtggccta tctcgac
//
LOCUS       DATAIX010000185         2620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_99703, whole genome shotgun sequence.
ACCESSION   DATAIX010000185 DATAIX010000000
VERSION     DATAIX010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99703"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VHM65_02790"
     CDS             complement(<1..180)
                     /locus_tag="VHM65_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704658.1"
                     /translation="MSDLLITSPANGRLRALVGLRRRRTREESGTTLVEGCEELTLAL
                     DAGVEPRVLFYCPELH"
     gene            complement(254..1081)
                     /locus_tag="VHM65_02795"
     CDS             complement(254..1081)
                     /locus_tag="VHM65_02795"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX2704659.1"
                     /translation="MPRSLPGPSARPRRLVALDLDGTTLDHSGGLSRAVRDAIRAVVF
                     AGTHVIIATGRSIVATVPVAEELGLMNGYAVCSNGAVTLRLDEAHPSGYDVIDTVTFD
                     PRPVLTLLRDRIPGALVAVEDLGVGFKVSARFPEGELGGEQTVVDWDHLVSSPATRVT
                     LRRPEGTPEAFMREVEAAGLHGVTYAVGWTAWLDISPEGVSKASALELLRRRLGVQPW
                     DTVAVGDQRNDVEMLRWAARGVAMGNAPDEVKAVADEVTADVAEDGLVPVLRSLLEA"
     gene            complement(1086..2381)
                     /gene="serS"
                     /locus_tag="VHM65_02800"
     CDS             complement(1086..2381)
                     /gene="serS"
                     /locus_tag="VHM65_02800"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX2704660.1"
                     /translation="MVVHIVQEVRREASDVVRASQRARGEDPALVDAILAADERRRSS
                     LAEFEALRAEQKAFGKRVAAAEGADKAALVAAAKATAERVKVLDAAAAAAQDELDTLL
                     RRAGNVIIEGVPAGGEDDYTVRETVGTPRDFAAEGFEPLDHVALGERLGAIDLDRGAK
                     VSGARFYFLTGPGARLELGLLHLAIDRAVAHGFTPMVTPTLVTSATMAGAGFLDAHAD
                     EVYHLPADDLYLTGTSEVALAGYHADEILDLTDGPRRYAGWSACYRREAGSHGKDTRG
                     IIRVHQFHKVEMFVYCRVEDAQAEHERLLAMEQDMLAAAELPYRIVDIAAGDLGGPAA
                     RKFDCEAWVPTQGRYRELTSTSNCTTFQARRLNTRERVPAELGGSGGTRVVATLNGTL
                     ATTRWLVAILENHQQADGSVLVPEALRPYVGLDLLTAVR"
     gene            2427..>2620
                     /locus_tag="VHM65_02805"
     CDS             2427..>2620
                     /locus_tag="VHM65_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704661.1"
                     /translation="MIDIRIVREASDVVRASQRARGEDPALVDAILAADERRRSSLAE
                     FEALRAGQKASGKRVAAAPRT"
BASE COUNT          361 a          929 c          975 g          355 t
ORIGIN      
        1 atgcagctcg gggcagtaga agaggacccg gggctccacc cccgcatcga gcgcgagggt
       61 gagctcctcg cacccctcga cgagggtcgt ccccgactcc tcgcgggtgc gtcgccgtcg
      121 cagcccgacg agcgccctga gccgcccgtt ggccggtgag gtgataagga ggtccgacat
      181 cgggctcatc atggcacccg atgagacgca gtggcccggt ccagccgaag tctgcgtcaa
      241 cgccggacct gggtcatgcc tcgagcagcg agcgcaggac cggcaccagt ccgtcctcgg
      301 cgacatctgc ggtgacctcg tcggcgaccg ctttgacctc gtcaggggcg ttgcccatcg
      361 cgacgccgcg cgcggcccag cggagcatct cgacgtcgtt gcgctggtcg ccgacggcga
      421 cggtgtccca cggctggacc cccagccgcc ggcgcagcag ctccagcgcc gaggccttgg
      481 agacgccctc cggggagatg tcgagccacg cggtccagcc cacggcatac gtcacaccat
      541 gcaggcccgc ggcctcgacc tcacgcatga acgcctcggg cgtgccctcg ggccggcgca
      601 acgtgacgcg agtggccggg ctggacacga ggtggtccca gtcgacgacg gtctgctcgc
      661 cgccgagctc gccttccggg aaccgcgcgc tgaccttgaa gccgacaccg aggtcctcga
      721 ccgcgacgag tgcccccggg atccggtcac gcagcagggt cagcaccggt cgcgggtcga
      781 aggtcaccgt gtcgatgacg tcgtacccgc tcgggtgcgc ctcgtcgagc cgcagcgtga
      841 ccgccccgtt ggagcacacg gcatacccgt tcatcagccc gagctcctcg gcgacgggga
      901 cggtcgcgac gatcgagcgc ccggtggcga tgatgacgtg cgtgccggca aagacgacgg
      961 cccggatggc gtcacgcacc gcgcgggaga ggccgccgga gtggtccagg gtcgtgccgt
     1021 cgaggtcgag ggccaccagg cgccgcggac gcgccgaggg cccgggaagg cttctgggca
     1081 cgggcctacc gcaccgccgt gaggaggtcg aggccgacgt aaggtcgcag cgcctcgggc
     1141 acgaggaccg aaccgtcggc ctgctggtgg ttctccagga tcgcgacgag ccaccgcgtg
     1201 gtggcgagcg tgccgttgag ggtcgccacg acgcgcgtgc cgcccgagcc gccgagctcg
     1261 gccgggacgc gctctcgcgt gttgagccga cgggcctgga acgtcgtgca gttcgacgtc
     1321 gaggtgagct cgcggtagcg cccctgggtc ggcacccacg cctcgcagtc gaacttgcgg
     1381 gcggccgggc cgccgaggtc gcccgcggcg atgtcgacga tgcggtaggg cagctcggcg
     1441 gcggcgagca tgtcctgctc catggcgagc aggcgctcat gctcggcctg ggcgtcctcg
     1501 acccggcagt agacgaacat ctccaccttg tggaactggt ggacccggat gatcccgcga
     1561 gtgtccttgc cgtggctgcc ggcctcgcgg cggtagcacg ccgaccaccc ggcgtagcgc
     1621 ctggggccgt cggtgaggtc gaggatctcg tcggcgtggt atcccgccag ggccacctcg
     1681 gacgtgccgg tgaggtagag gtcgtcggcg ggcaggtggt agacctcgtc ggcgtgcgcg
     1741 tcgaggaacc cggcgccggc catcgtcgcc gacgtcacca gcgtcggggt caccatgggg
     1801 gtgaagccgt gcgccacggc gcggtcgatg gcgagatgga gcaggccgag ctcaagtcgg
     1861 gcgcccgggc cggtgaggaa gtagaaccgg gcgccggaga ccttcgcgcc ccggtcgagg
     1921 tcgatggccc cgaggcgctc gccgagggcg acgtggtcga gcggctcgaa tccctcggcc
     1981 gcgaagtccc gtggcgtccc gacggtctcg cgcacggtgt agtcgtcctc accaccggcg
     2041 gggacgccct cgatgatgac gttgccggcc cggcgcagga gggtgtccag ctcgtcctgg
     2101 gcggctgcgg cagccgcgtc gagcaccttg acccgctcgg cggtcgcctt ggcggccgcg
     2161 acgagcgcgg ccttgtcggc tccctccgcg gcggcgacgc gcttgccgaa ggccttctgc
     2221 tcggcccgga gcgcctcgaa ctcggcgagg gaggatcggc ggcgctcgtc ggcggcgagg
     2281 atcgcgtcga cgagcgccgg gtcctctcca cgggcacgct gcgaggcgcg gaccacgtcg
     2341 gaggcctcgc ggcgtacttc ctgcacgatg tgcaccacca cggaaaggac atcggcgcga
     2401 tggccccgcc tcgataggct ggcccggtga tcgacatccg catcgtgcgc gaggcctccg
     2461 acgtggtccg cgcctcgcaa cgtgcccgtg gggaggaccc ggcgctcgtc gacgcgatcc
     2521 tcgctgccga tgagcgccgt cgatcctccc tcgccgagtt cgaggcgctg cgggccggcc
     2581 agaaggcctc cggcaagcgc gtcgccgcag cgcctcgaac
//
LOCUS       DATAIX010000186         1661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_10312, whole genome shotgun sequence.
ACCESSION   DATAIX010000186 DATAIX010000000
VERSION     DATAIX010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10312"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..374)
                     /locus_tag="VHM65_02810"
     CDS             complement(87..374)
                     /locus_tag="VHM65_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005326607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX2704662.1"
                     /translation="MHETMATVLHTTALPDATEQVVEELQWQERALCAQTDPEAFFPE
                     KGGSTREAKRVCLSCDVRVECLEYALANDERFGIWGGLSERERRRVKKRAV"
     gene            690..>1661
                     /gene="cofD"
                     /locus_tag="VHM65_02815"
     CDS             690..>1661
                     /gene="cofD"
                     /locus_tag="VHM65_02815"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX2704663.1"
                     /translation="MRITALAGGIGGARFLRGLLHHLATIDPSAQVTVIGNTADDVTL
                     HGLRVCPDLDTVMYTLGGGIHPEQGWGRDDEQFTVAEELAAYGAQPQWFTLGDRDLAT
                     HLVRTRMLGAGLTLSQATAALCERWQPGVRLLPMSDDRVETHIMIDDDEAAPTEDGSP
                     ATRAVHFQEWWVRLHAAVPARQILAVGIEQSKPAPGVLDAIREADVVLLPPSNPVVSI
                     GTILGVPGVREALRGTRAPVVGVSPVIAGAPVRGMADACLTAIGVPTSAGAVAGLYRD
                     FLGGWLVDDADAAFATVPPSGLIVRARPLLMTDVEASAKIAAAALDLA"
BASE COUNT          244 a          599 c          561 g          257 t
ORIGIN      
        1 ggtcggcgcc tgcggcaagg tcggtggtca caccgaggtt gggggtcaca ccgaagttgg
       61 gggtcacacc gtcgatgatt acctgatcag accgctcgtt tcttgacgcg acgccgttcg
      121 cgctcggaaa gtcctcccca gatcccgaat cgctcgtcgt tggcgagcgc gtactccagg
      181 cactccaccc gcacgtcgca ggacagacag acccgcttgg cctcgcgggt cgacccaccc
      241 ttctccggga agaaggcctc cgggtcggtc tgggcgcaca aggcgcgttc ctgccactgc
      301 agctcttcca cgacctgttc ggtcgcgtcc ggcagcgccg tggtgtgcag cactgtcgcc
      361 atggtctcgt gcacctggcg tcctcctcga ccttggcccc acatcgggac tcccccccat
      421 aacctgccgc gtgtcaaaca ctcaaagtgt tcgtatgagc accctgtgtg ttcgcgcagg
      481 tgaaacagta atggaattac acgcgtgtcg tgcgcggaac gtcaagcagg gcggtggtat
      541 ccccccgaac agatgacccc gagtggcctt catcggcgag tcgtccacag atgcacagtc
      601 ggtgtccaca gccgatcgcc cgtcgtccac aatccgtcaa caggcacccg gccgagcgcc
      661 acggccccgc gatgcgagga ttggccacca tgcgcatcac agcactcgcg ggcggcatcg
      721 gcggggccag gtttctgcgg ggcctgctcc accacctcgc cacgatcgac ccgtcggccc
      781 aggtcaccgt gatcggcaac actgcggacg acgtcaccct gcacggcctt cgggtctgcc
      841 ccgacctcga caccgtgatg tacaccctcg gcggcgggat ccaccctgaa cagggttggg
      901 gtcgcgacga cgagcagttc acggtggccg aggagctggc cgcctatgga gcgcagccgc
      961 agtggttcac cctcggcgac cgcgacctgg ccacccacct cgtgcgcacc cggatgctcg
     1021 gggcggggct gaccctttcg caggccacgg cggcactgtg cgagcgctgg cagcccggcg
     1081 tccggctgct gccgatgagc gacgatcggg tcgagaccca catcatgatc gacgacgacg
     1141 aggcggcgcc caccgaggac ggcagcccgg ccacccgggc ggtgcacttc caggagtggt
     1201 gggtgcggct gcacgcggcg gtgccggccc ggcagatcct cgcggtcggg atcgagcagt
     1261 ccaagcccgc gccgggggtg ctggacgcga tccgggaagc ggacgtcgta ctactgccgc
     1321 cgagcaaccc ggtggtctcg atcggcacca tcctcggcgt cccaggcgtg cgtgaggcgc
     1381 tgcgcggcac ccgggccccg gtggtggggg tctcgccggt gatcgccggc gccccggtcc
     1441 gcgggatggc cgacgcctgc ctgaccgcga tcggggtgcc gacctcggcc ggcgcggtcg
     1501 ccgggctcta ccgcgacttc ctcggcggct ggctggtgga cgacgcggac gctgcgttcg
     1561 ccacggtgcc accctccggg ttgatcgtcc gggcccgccc gctgctgatg acggacgtcg
     1621 aggcctcggc gaagatcgcc gcggccgccc tcgacctggc c
//
LOCUS       DATAIX010000187         3609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_40484, whole genome shotgun sequence.
ACCESSION   DATAIX010000187 DATAIX010000000
VERSION     DATAIX010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40484"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(464..721)
                     /locus_tag="VHM65_02820"
     CDS             complement(464..721)
                     /locus_tag="VHM65_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704664.1"
                     /translation="MMTSTPSYEHRVANATGPSDGPDDGLTVAQLRRLLPQLPSQARV
                     VIGVRDDHHFDVLLGELPVLSASAVNGPARCALVLQVDGLS"
     gene            931..1530
                     /locus_tag="VHM65_02825"
     CDS             931..1530
                     /locus_tag="VHM65_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2704665.1"
                     /translation="MTGPHERVTTRMRVHEATLELFLAHGYEGTSLQTIADRLSITKA
                     AIYYYYRTKGDLLEALLSPVWDELTQLISEAKTSLAPARHDLGLLEAYVDMLIRQRRL
                     VPLLDRDPGVRSQPAVQSGLLPLRRRLEQLILGDEADEDSQLGLLIMRAGFCEVLVAF
                     TSWDDEDLRVALLPVAHALLGRRRLHSRPKNSAIRPHTA"
     gene            1505..2419
                     /gene="eccD"
                     /locus_tag="VHM65_02830"
                     /pseudo
     CDS             1505..2419
                     /gene="eccD"
                     /locus_tag="VHM65_02830"
                     /inference="COORDINATES: protein motif:HMM:NF039223.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion integral membrane protein
                     EccD"
     gene            2416..>3609
                     /gene="mycP"
                     /locus_tag="VHM65_02835"
     CDS             2416..>3609
                     /gene="mycP"
                     /locus_tag="VHM65_02835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion-associated serine protease
                     mycosin"
                     /protein_id="HEX2704666.1"
                     /translation="MTPRSWSRSRGAAGAARAALAAATVTVLNTGIAAPATPARAGPT
                     DGPTAYQQVKRCISDGRALEPLASAVSPAQRLLGYTQLWALSRGDGVTVAVIDTGVNP
                     SPAFGTRLVSGGDLVQPAATRAGAPQPGLDDCDGHGTLVAGIIAATPDPKTGFAGVAP
                     GARIVSIRQNSEAFAPEGSASSAEGAGTSTTLAQALDLAVAAGVDVDVVNISAAHCGP
                     AITVGDPALTSAVERAVQRDVVVVVAAGNLGSGGDCSTQNTPGQRPVTGATPSSIPAA
                     LTVGSVTTAGAPADFSLASEWVDVAAPGERIISTNPHREQTGQVDSVVGAEGVVPIQG
                     TSFSAPYVAGVAALVRARFPDLTAEQVMHRIAVTADHPAGPGERTIYVGFGTINPRRA
                     LTAVLA"
BASE COUNT          526 a         1366 c         1173 g          544 t
ORIGIN      
        1 tcacccccgt ccgcgcaaca atcgccgccg tgtcaccagg aaacgagaac cccgccatca
       61 cgccgccccg ggtaccggga gggttgtgac cgtggccgcg accttcgaca gcgactcgta
      121 tcccccctgc agggtgcacc agtgagcacc agacccagca tcatcctggc cccagcctgg
      181 ccgatattcc ttgggtcaca ccgggatctt gcgttggcgc gctgacgccc gcctgtggcc
      241 ctatcgacgg accgacctga gttgctcgac cctcggcgag ccggtcagac catccggcgt
      301 cgaaaggaca cggcacaagc gcaaccacat ccgccaactc gaggccctcg gctacaaggt
      361 caccctgcaa cccgccgcct gacagtccag cccccgaaga ccagatctca accccgcccg
      421 ctcatcctca tttccgatca gggggtatcc agcagttgca ccgtcaggac aggccgtcca
      481 cctggagcac gagcgcgcag cgggccgggc cgttgactgc ggacgcactc aacacgggca
      541 gctctcctag cagcacgtcg aagtgatggt catcgcggac gccgatcacc acgcgcgctt
      601 gcgagggtaa ctgcggcagg agccgtcgta actgcgcgac cgtcaacccg tcatctgggc
      661 cgtctgaggg gcccgttgcg tttgcgaccc gatgctcata gctcggtgtc gaggtcatca
      721 tctcggtctc cttccctcga ggaggcgctt caccggccac tacagttacg tcggcgtatg
      781 cacccggtgc ggcccgagcc gcagatgcta gccatactgc tagtctccac atccaggata
      841 cctagccgtt cggctaggta tcctctggaa agcagccgac ttgccgtggc acatatcaca
      901 gacctcaccg ccagactggg agcaagtacg atgaccggtc cgcacgagcg ggtcaccacc
      961 cgaatgagag tgcatgaggc gacgcttgag ctgttcctag cgcacggata cgaaggcacg
     1021 tcgcttcaaa cgatcgccga ccgtctgagc atcaccaagg cggctattta ctactactac
     1081 cgcacgaagg gcgacctgct cgaggcgctg ctgagtcccg tgtgggacga gctgacgcag
     1141 ctgatctccg aggcgaagac gtccctcgct cctgcccggc acgaccttgg gctgctggaa
     1201 gcctacgtcg acatgctcat tcgacaacgc cggttggtgc ccctgctgga ccgggatccg
     1261 ggtgtccgct cccagccagc ggtccagtcc ggactccttc cgttgcgacg ccgactggag
     1321 cagttgatcc tcggggacga agccgacgag gactcgcagc tggggctgct gatcatgcga
     1381 gcgggtttct gtgaggttct cgtcgccttc accagttggg acgacgagga cctgcgggta
     1441 gcgctgctac ctgttgccca cgcgctgttg ggccgtaggc gcctgcactc gcggcccaag
     1501 aacagtgcca ttcggccgca tacggcgtga tcccacaacc cgccgcccgc cgcccactgc
     1561 ggccggcgac gcaacgttgc cctcgaagaa cgccactacg ccgtggagct gttccaggag
     1621 cgccacctgc gccataccgg gtgcccctgg ttaccccggc gcgctcctag gctcggcgtt
     1681 cgccctgacg tatgccgtcg cggccggccc gctgctcgcg accggcaccg tcgtcttctc
     1741 ggctctggcc acgtcggcgt tcttcgctgt ctccggggca ctcggcgggc tcctcctcga
     1801 tgcccgggcc tcccacgtcg ggaccgtggt ctcggcctgg gctctcgccg cgatgaccct
     1861 gctgccgtgg atggcggtcc ggatggcccg gctgccgatg ccggtcatcc ccaccacggc
     1921 ggaccacctg cgcgacagcg cgctcggcgt ggacttcgcg gccacgggcc gccgagcgac
     1981 cgtggccgcc gagtacctcg acgggaccct gaccggatgc gcgctcgtcg ccgcgggcgg
     2041 ggcggccctg acgatggcga ctggcacggt gttcggggtg ctgtaggccg ccgttgccct
     2101 cgccgtgctc atgctgcggg tccggtccct cgcgggccag ctgtcccgga ccgcgttgct
     2161 cgcggccggg ctcgccggca gcgccgtggg catcgccctc gcggcggacc agatgccccg
     2221 tgagtcgacc ctcggcctgg tggccctgtc cctggccggg agcgccacgg cggtcctgct
     2281 caccgtggtc gggccacgac gccggctcag cccgatgacg gggcgaagca tcgacatcgc
     2341 cgagagcctg ttgctcgcct ccgtcctgcc actcgcagtc ggcgcgatcg acctctactc
     2401 gacggtgcgc cactggtgac cccgcggtcg tggtcgcggt cgcggggggc cgccggggcc
     2461 gcccgggccg cactagccgc agccaccgtc acggtcctga acacgggcat cgcggcaccg
     2521 gccacaccgg cgcgagcggg gccgaccgac gggccgacgg cataccagca ggtcaagcgc
     2581 tgcatctccg acggccgtgc cctcgaaccg ctcgcgtccg cggtctcccc ggcccagcgg
     2641 ctcctcggct acacccagct gtgggccctg tcccggggtg acggggtcac cgtcgcggtg
     2701 atcgacaccg gggtcaaccc cagccccgcg ttcggcaccc gcctcgtcag tggcggcgac
     2761 ctggtccagc ccgcggcgac ccgcgctggt gcgccgcagc caggcctcga cgactgcgac
     2821 gggcacggaa ccctcgtcgc cgggatcatc gcggccacgc ccgacccgaa gaccggcttc
     2881 gccggggtcg cccccggggc gaggatcgtg tcgatccggc agaacagcga ggcgttcgcc
     2941 cccgaaggct cggcctcgtc cgccgaggga gccgggacca gcaccaccct ggcccaggct
     3001 ctcgacctgg ccgtcgcggc cggcgtcgac gtcgacgtcg tcaacatctc cgccgcgcac
     3061 tgcggcccgg cgatcacagt cggcgacccg gccctgacct cggcggtcga gcgagccgtc
     3121 cagcgcgatg tcgtcgtcgt cgtcgcggcc ggcaacctgg gctccggcgg cgactgctcc
     3181 acccagaaca cacccggaca gcgaccggtc acgggtgcga cgccatccag catcccggca
     3241 gcgctgaccg tcggctcggt gacgacagcc ggagcgccgg cggacttctc gctggccagc
     3301 gagtgggtcg atgtcgcggc tcccggcgag cgcatcatct cgacgaaccc ccaccgcgag
     3361 cagaccggcc aggtggactc ggtcgtcggc gccgagggcg tcgtcccgat ccaggggacg
     3421 agcttctccg cgccctacgt cgccggcgtc gccgccctcg tccgcgcccg gttccccgac
     3481 ctgaccgccg aacaggtcat gcatcggatc gcggtgaccg cggaccatcc ggccggtccg
     3541 ggcgagcgca cgatctacgt cggtttcggg accatcaacc cccggcgcgc cctcaccgcg
     3601 gtgctggcg
//
LOCUS       DATAIX010000188         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_159857, whole genome shotgun sequence.
ACCESSION   DATAIX010000188 DATAIX010000000
VERSION     DATAIX010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159857"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..785
                     /gene="nusG"
                     /locus_tag="VHM65_02840"
     CDS             210..785
                     /gene="nusG"
                     /locus_tag="VHM65_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX2704667.1"
                     /translation="MRARPGDWFVVHSYAGYENRVRANLETRIQTLNMEDYIFEIEVP
                     MEEVTEIKNGQKKLVRRVRMPGYVLVRMDMTDASWGAVRHTPGVTGFVGNAHQPVPLS
                     IDEVVTMLAPDLEAAMAGPEGTLRRSKPAEVRLVDFEVGESVTVMEGPFESLSATISE
                     INPDTQKLKVLVSIFGRETPVELSFGQVAKI"
     gene            853..1290
                     /gene="rplK"
                     /locus_tag="VHM65_02845"
     CDS             853..1290
                     /gene="rplK"
                     /locus_tag="VHM65_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX2704668.1"
                     /translation="MPPKKKKISGFIKLQINAGAATPAPPVGPALGQHGVNIMEFVKA
                     YNAATEHQRGNVIPVEITVYEDRSFTFVTKTPPAAELIKKAAGVPKGSGEPHKTKVAT
                     LSADQVRQIAADKMEDLNANDIEAAAKIIAGTARSMGILTEGY"
     gene            1450..>2111
                     /gene="rplA"
                     /locus_tag="VHM65_02850"
     CDS             1450..>2111
                     /gene="rplA"
                     /locus_tag="VHM65_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX2704669.1"
                     /translation="MKRSKAYQAAAEKIVAGRVYTPLEAVRLAKETSPTKYDATIEVA
                     FRLSVDPRKADQMVRGTVNLPHGTGKTARVLVFANAEKADAAREAGADYVGSDDMLEK
                     VSGGWLDFDAVVATPDMMGKVGRLGKVLGPRGLMPNPKTGTVTMDVAKAVTEIKGGKI
                     EFRVDKHANLHFIIGKSSFSDTALVQNYAAALDEVLRLKPSAAKGRYLKKATIATTMG
                     PG"
BASE COUNT          405 a          766 c          680 g          260 t
ORIGIN      
        1 cccctaggaa gtaggtcatc agcgtgtccg acgaggtcac cgagttcgag gagaccgcgg
       61 ctgtggagca cgacgagccg cggactgtcg ccgcccccgc ggtcgaggcg gcgcccgagg
      121 ccgaagcggc gcccgaggcc gaggagcccg cagccgaccc ggtggccgag gagcccgcag
      181 ccgacccggt ggccgagttc aaggccggcc tgcgggcccg tcccggggac tggtttgtcg
      241 tgcactccta cgcggggtac gagaaccggg tccgggccaa cctcgagact cgcatccaga
      301 ccctcaacat ggaggactac atcttcgaga tcgaggtccc catggaagag gtgaccgaga
      361 tcaagaacgg ccagaagaag ctcgtccgcc gggtgcggat gcccggctac gtcctcgtcc
      421 ggatggacat gaccgacgcg agctgggggg cggtccgtca taccccgggt gtcaccggct
      481 tcgtcggcaa cgcgcaccag cccgtcccgc tgagcatcga cgaggtcgtc accatgctcg
      541 ccccagacct cgaggcggcc atggccggcc ccgagggcac cctgcggcgc tccaagcccg
      601 ccgaggtccg gctcgtcgac ttcgaggtcg gcgagtccgt caccgtcatg gagggcccct
      661 tcgagtcgct ctccgccacg atctccgaga tcaaccctga cacgcagaag ctcaaggtgc
      721 tggtctcgat cttcggccgg gagaccccgg tcgagctgtc cttcggccag gtcgccaaga
      781 tctgaccggc agacgcgcac gacaacggcc caacacacgg ctcacaccca gcacagctaa
      841 ggaccccaac ccatgcctcc caagaagaag aagatctccg gcttcatcaa gctgcagatc
      901 aacgcgggcg cggccactcc ggccccgccg gtcggcccgg cgctcggcca gcacggcgtc
      961 aacatcatgg agttcgtcaa ggcctacaac gccgcgaccg agcaccagcg cggcaacgtc
     1021 atcccagtcg agatcaccgt ctatgaggac cgttccttca ctttcgtcac caagaccccg
     1081 ccggccgcgg agctcatcaa gaaggccgcg ggcgtgccga agggctcggg tgagccgcac
     1141 aagaccaagg tcgccacgct gtcggccgac caggtccgcc agatcgccgc cgacaagatg
     1201 gaggacctca acgccaacga catcgaagcc gcggccaaga tcatcgccgg caccgcccgc
     1261 tccatgggca tcctgaccga gggctactag ggcggtcagg ggctccgccc ccgactgtcc
     1321 cgaggacccg cgcggtcgcc ccagaccgca cgggcacgac atacctcccg gtatgacgag
     1381 tggcagggcc gagcgcggcc cgcgaaccac gactccacag cacgaccagc agaagccata
     1441 ggagcagcaa tgaagcgcag caaggcctac caggccgccg cagagaagat cgtcgcgggc
     1501 agggtctaca ccccgctcga ggccgtccgc ctcgcgaagg agacgtcccc gacgaagtac
     1561 gacgccacca tcgaggtcgc cttccggctg tcggtcgacc cgcgcaaggc cgaccagatg
     1621 gtgcgcggca ccgtcaacct gccgcacggc accggcaaga ccgcccgggt cctggtcttc
     1681 gccaacgccg agaaggccga cgccgcgcgt gaggcgggcg ctgactacgt cgggtccgac
     1741 gacatgctcg agaaggtctc gggcggctgg ctcgacttcg acgccgtcgt cgcgacgcca
     1801 gacatgatgg gcaaggtcgg ccggctcggc aaggtcctcg gcccccgtgg cctcatgccc
     1861 aacccgaaga cgggcaccgt gacgatggac gtcgccaagg cggtcaccga gatcaagggc
     1921 ggcaagatcg agttccgcgt cgacaagcac gcgaacctgc acttcatcat cggcaagtcg
     1981 tcgttcagcg acaccgccct ggtgcagaac tacgcggccg ccctggacga ggtgctgcgg
     2041 ctcaagccgt cggccgccaa gggccggtac ctgaagaagg ccaccatcgc caccaccatg
     2101 gggcccggga t
//
LOCUS       DATAIX010000189         2243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_242980, whole genome shotgun sequence.
ACCESSION   DATAIX010000189 DATAIX010000000
VERSION     DATAIX010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242980"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1243
                     /locus_tag="VHM65_02855"
     CDS             113..1243
                     /locus_tag="VHM65_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2704670.1"
                     /translation="MLPYGHQSIDESDVAAVVAVLHGDWLTTGPAVASFERGLAALAG
                     TPDAVSVTSGTAALHVAYAALGVGPGDEVVTTPLTFVATASCASLLGATVVFADVTED
                     TGNLDPAAVGAALTPRTRVVTGVDYGGHPIDAPGLRSAIRDSGVVLLEDAAHSVGGSL
                     DGVPVGSLADVTTFSFFPTKNLTTAEGGAVVAVDPGVVARARTFRTIGLVFDGESLRL
                     PDEGPWHKEVHSFGLNYRLPDVLAALGSSQLRRLGAFKARRQELTDRYNRALSGIDGL
                     RLPAHRPEADPVWHLYPLRVPDGRRRALYDHLRAQGIGVQVNYLPVYWHPVYADLGYQ
                     RGMCPVAEEYYRQELSLPLFHGLSDADVDRVIDAVRGFFGHR"
     gene            1240..2073
                     /locus_tag="VHM65_02860"
     CDS             1240..2073
                     /locus_tag="VHM65_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704671.1"
                     /translation="MTDPGAQAGSRHAAEASRAVLGRGWIYTAGTASPILAQLVVTPA
                     VTRLLGPDGGWGRVATGLVLVQVTMMLASLGFPSVITRQALLAGGGVAGARSLFLRGS
                     LLTCAVSGVALVTRRWWGAGFGGLEADLALVAIGAGTLFVIVENGQALLRALDRPVAF
                     VALSAVASLGGPLLGLALVLAGGEPGWLHYLLGLAGATRWAPRWRHTSACVGFAPTAA
                     ATSAPRSGWGSPSCRTSSRSTWPTERWSCSPAGSSATAMPGGSSSRSSSARRPGSSSV
                     P"
BASE COUNT          234 a          867 c          794 g          348 t
ORIGIN      
        1 ccccgccggc ggggtcccgg tgcccgaggg cttccactac acgtccgaca ccaacgaccg
       61 gtggttcacc cgtgaggaga tcgcccggct cctcgagtcg gacgtctagg ccgtgcttcc
      121 ctacggccat cagtcgatcg acgagtccga cgtcgctgcc gtcgtggccg tgctgcacgg
      181 cgactggctc accaccggcc cggctgttgc gtccttcgaa cgcggcctag ccgcgctcgc
      241 cgggaccccc gacgcggtct cggtgacgtc cgggaccgcg gccctgcatg tcgcctacgc
      301 cgccctgggc gtcgggccgg gcgacgaggt ggtgaccacg ccgctcacgt ttgtggccac
      361 cgccagctgc gcgtcgctgc tgggcgccac cgtcgtcttc gccgacgtca ccgaggacac
      421 gggcaacctc gacccggcag cggtcggcgc cgcgctgacc ccgcggacca gggtcgtcac
      481 gggggtcgac tacggcgggc atccgatcga cgcaccgggg ctgcgcagcg ccatccggga
      541 ctccggggtg gtgctgctcg aggacgcggc gcactcggtc ggtgggtcgc tcgacggcgt
      601 gccggtcggc tcgctcgccg acgtcacgac cttctccttc ttcccgacca agaacctcac
      661 gaccgccgag ggcggcgccg tcgtcgcggt cgacccgggc gtcgtcgccc gggcgcggac
      721 cttccgcacg atcgggctcg tcttcgacgg agagagcctg cgcctgcccg acgagggacc
      781 ctggcacaag gaggtccact cgttcgggct caactaccgg ctgcccgacg tcctcgccgc
      841 cctggggtcg agccagctgc gccgcctcgg ggcgttcaag gcccggcgcc aagagctcac
      901 ggaccgctac aaccgcgcgc tgtccggcat cgacgggttg cgcctgccgg cccaccgccc
      961 ggaggccgac ccggtgtggc atctgtaccc gctgcgtgtc cccgacgggc gccgccgtgc
     1021 tctctacgac cacctgcgcg cccagggcat cggggttcag gtcaactacc tgcccgtcta
     1081 ctggcacccg gtctatgccg acctgggcta ccagcgcggg atgtgcccgg tcgccgagga
     1141 gtactaccgc caggagctct cgctgccgtt gttccacggc ctgtccgacg ccgacgtgga
     1201 ccgggtcatc gacgcggtcc gcgggttctt cgggcaccgg tgacggaccc gggcgcgcag
     1261 gccgggagcc ggcacgcggc cgaggcgagc cgtgcggtcc tgggccgagg ctggatctac
     1321 acggccggga ccgcctcccc gatcctcgcc cagctcgtcg tcacccccgc cgtcacgagg
     1381 ctcctcggtc cggacggtgg gtggggccgc gtcgcgaccg gcctggtgct cgtccaggtg
     1441 acgatgatgc tcgccagtct cggcttcccc tcggtcatca ccaggcaggc cctgctggcc
     1501 gggggcgggg tcgccggggc ccgcagcctg ttcctgcgcg gctccctcct gacctgcgcc
     1561 gtgtccggcg tcgcgctcgt gacccggcgc tggtggggcg ccgggttcgg cgggctcgag
     1621 gccgaccttg ccctcgtggc catcggggcc gggacgctgt tcgtcatcgt cgagaacggg
     1681 caggcgctcc tgcgggccct cgaccgtccc gtggccttcg tcgccctgtc ggcggtggcc
     1741 agcctcggtg gcccgctgct cgggctggcc ctcgtcctcg ccggaggcga gcccggctgg
     1801 ctgcactacc tgctcggcct cgccggggct acgcgctggg cgccgcgctg gcggcatacc
     1861 tctgcctgcg tggggttcgc acccaccgcg gcggcgacgt ccgcgccgcg ctccggctgg
     1921 ggctccccgt cctgccgcac ctcgtctcgc tctacctggc caacggagcg ctggtcctgc
     1981 tcgccggccg gttcttcggc gaccgcgatg ccgggcggat ccagctcgcg atcctcgtcg
     2041 gcgcgtcgcc cggggtcctc atcggtgccc tgaacaacgc ctgggcaccg gcgatctacc
     2101 gggtgccgac cgcgcaccgg gggcagacgg tcgaacggac cgcccgtgac gtggccgcga
     2161 tcgccgccct gctctgtggc ggggtgtcgc tcctgtctcc gtggctgctg cgactgctcg
     2221 ccgatgagcg ctatgacctg ggc
//
LOCUS       DATAIX010000190         4338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_100012, whole genome shotgun sequence.
ACCESSION   DATAIX010000190 DATAIX010000000
VERSION     DATAIX010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100012"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..820)
                     /locus_tag="VHM65_02865"
     CDS             complement(77..820)
                     /locus_tag="VHM65_02865"
                     /inference="COORDINATES: protein motif:HMM:NF005746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX2704672.1"
                     /translation="MKITLKIWRQAGPSAAGSMVTYQADDVSPDMSFLEMLDVLNEDL
                     QARGEEPVAFDSDCREGICGMCGLVINGHPHGPEVTTTCQLHMRSFSDGDTVTIEPWR
                     AEAFPVLKDLVVDRGAFDRIIQAGGFISVNTGAAPEAHSVPVPKNKADRAFDAATCIG
                     CGACVAACPNASAMLFVGAKVTHLGELPQGQPERDARVVTMLSQHDHEGFGGCTNVGA
                     CADACPKEIPLDVISQLNSDLRHSMFKGG"
     gene            complement(817..2784)
                     /locus_tag="VHM65_02870"
     CDS             complement(817..2784)
                     /locus_tag="VHM65_02870"
                     /inference="COORDINATES: protein motif:HMM:NF005749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEX2704673.1"
                     /translation="MTTTSGTLVHGLYHEGAPIKDTKAPSGPIEDRWATRQFEAKLVN
                     PANRRKLSVIIVGTGLAGASAGATLGEAGYQVKSFCYQDSPRRAHSIAAQGGINAAKN
                     YKEDGDSVFRLFYDTVKGGDYRSRESNVFRLAEVSTNIIDQCVAQGVPFAREYGGLLD
                     NRSFGGVQVSRTFYARGQTGQQLLIGAYQALERQIAEGAVEMFTRHEMLELVVVDGRA
                     RGIIVRDMVSGEIETHLADAVVLASGGYGNVFYLSTNAMGCNVTATWRAHRKGAHFAN
                     PCFTQIHPTCIPVSGDHQSKLTLMSESLRNDGRIWVPKDKADKDKDPRQIPEADRDYY
                     LERIYPAFGNLVPRDIASRAAKYQCDDGKGVGPGGLGVYLDFAEAIARMGRPAVEEQY
                     GNLFDMYARITGENPYETPMRIYPAVHYTMGGLWVDYDLQSSIPGLFVTGEANFSDHG
                     ANRLGASALMQGLADGYFVLPNTIRDYLASGPYPTVDGNAPEVLEARESVEQRLAHFL
                     SCNGTRTVDSYHKELGHLMWEYCGMERTEEGLALAIERIRALRKEFWADVRVLGVNEG
                     VNQSLEKAGRVADLLELGELMCIDALHRRESCGGHFRAESQTEEGEALRHDDLYAYVA
                     AWEWGGINRPPTLHKEDLVYEAVELKQRSYK"
     gene            complement(2809..3525)
                     /locus_tag="VHM65_02875"
     CDS             complement(2809..3525)
                     /locus_tag="VHM65_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HEX2704674.1"
                     /translation="MATSTLSPAQVKARRTTVAMKMLMATTGALFVFYVLMHMYGNLK
                     IFGGQDAFDTYAHHLRTLGEPILPYEGFLWVFRLVLVASLVGHAYSAFYLWTRARSAR
                     PTGYAVRLAGAAAWRSQAMRWGGVALLLFLVFHLIQFTISKVNFNSGVAAAKIGDSPY
                     QLVVASFQLWWVVLVYVLALLALGLHLEHGVWSGAQTLGWTGSPRARGAAKMAARLIA
                     AVVVVGFALPPLSILFGIVK"
     gene            3605..>4338
                     /locus_tag="VHM65_02880"
     CDS             3605..>4338
                     /locus_tag="VHM65_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX2704675.1"
                     /translation="MSEPIDGFWAVIPAGGAGTRLWPLSRAASPKFLHDLTGSGRTLL
                     QSTWDRLTPLAGDQVVVVTGVAHAAAVAGQLPGMPAAHLLVEPSPRDSMPAIALAAAL
                     VERWDPDGVIGSFAADHVIGDAVAFHACVREAVSVARGGAVVTLGVTPTHPATGFGYI
                     RTGEALAGFPTARSVVRFVEKPDAARASAYVASGEFLWNAGIFVVQASVLLDVLASYH
                     PDLARGARAIAADLRRLGEVWPTMAR"
BASE COUNT          638 a         1434 c         1579 g          687 t
ORIGIN      
        1 gacatggccc ggaacgacgc gtcggtgccg tcggccgggt cagtgctcag cgcccggacg
       61 gtatggcgtg cgctggtcag ccgcccttga acatcgagtg acgcaggtcc gagttgagct
      121 gggagatcac gtcgagcggg atctccttgg ggcaggcgtc cgcgcaggcg ccgacattgg
      181 tgcagccacc gaagccctcg tggtcgtgct ggctcagcat cgtgaccacg cgcgcgtcac
      241 gctcgggctg gccctggggg agctcgccca ggtgggtcac cttggcgccg acgaagagca
      301 tggcggaggc gttggggcag gccgcgacgc acgcaccgca gccgatacag gtcgccgcat
      361 cgaaggcccg gtcggccttg ttcttgggga cgggcaccga gtgggcctcg ggggcggcgc
      421 cggtgttgac cgagatgaag ccaccggcct ggatgatccg gtcgaaggcg ccccggtcga
      481 cgacgaggtc cttgaggacc gggaacgcct cggcccgcca cggctcgatc gtgacggtgt
      541 cgccgtcgga gaacgaacgc atgtgcagct ggcaggtggt ggtcacctcg ggcccgtgcg
      601 ggtggccgtt gatgacgagg ccgcacatgc cacagatgcc ttcgcggcag tcgctgtcga
      661 aggccacggg ctcctcgccg cgagcctgca ggtcctcgtt gagcacgtcg agcatctcga
      721 ggaaggacat gtcgggcgag acgtcgtcgg cctggtaggt caccatcgac cctgccgcgc
      781 tgggtccggc ctggcgccag atcttgaggg tgatcttcac ttgtagctcc gttgcttcag
      841 ctcgacggcc tcgtacacca ggtcttcctt gtgcagcgtc gggggacggt tgatgccgcc
      901 ccactcccac gccgcgacat aggcgtagag gtcgtcgtgg cgcagcgcct cgccctcctc
      961 ggtctgcgac tcggcgcgga agtgcccacc gcacgactcg cgccggtgca gcgcgtcgat
     1021 gcacatgagc tcgccgagct cgagcaggtc ggccacccgg ccggccttct cgagcgactg
     1081 gttgacgccc tcgttgacac cgaggacgcg cacgtcggcc cagaactcct tgcgcagggc
     1141 gcggatccgc tcgatcgcga gggccagtcc ctcctcggtg cgctccatgc cgcagtactc
     1201 ccacatgagg tggcccagct ccttgtggta ggagtccacg gtgcgggtgc cgttgcacga
     1261 caggaagtgc gcgagccgct gctcgaccga ctcgcgggcc tcgagcacct cgggagcgtt
     1321 gccatcgacc gtcgggtaag ggccgctcgc gaggtagtcc cggatcgtgt tgggcaggac
     1381 gaagtagccg tcggcgaggc cctgcatgag cgccgaggca cccaggcggt tggcgccgtg
     1441 gtcggagaag ttggcctcgc cggtgacgaa caggccgggg atcgaggact gcaggtcgta
     1501 gtcgacccac agcccgccca tcgtgtagtg gaccgcgggg tagatgcgca tcggggtctc
     1561 gtaggggttc tccccggtga tccgcgcgta catgtcgaaa aggttgccgt actgctcctc
     1621 gaccgcgggc cggcccatcc gggcgatggc ctcggcgaag tcgaggtaga caccgagccc
     1681 ccccgggccg acgcccttgc cgtcgtcgca ctggtacttc gccgcccgcg aggcgatgtc
     1741 gcgggggacg aggttgccga acgccgggta gatccgctcg aggtagtagt cgcggtcggc
     1801 ctcggggatc tggcgcgggt ccttgtcctt gtcggccttg tccttgggca cccagatgcg
     1861 cccgtcgttg cgcagcgact cggacatcag cgtgagcttg ctctgatggt cgccggacac
     1921 cgggatgcag gtcgggtgga tctgcgtgaa gcaggggttg gcgaagtggg cgcccttgcg
     1981 gtgggcccgc cacgtcgcgg tgacgttgca ccccatggcg ttggtcgaca ggtagaagac
     2041 gttgccgtag ccgcccgagg cgagcacgac ggcatcggcg agatgggtct cgatctcacc
     2101 gctgaccatg tcgcgcacga tgatgccgcg ggcgcggccg tcgacgacga cgagctcgag
     2161 catctcgtgc cgggtgaaca tctcgaccgc gccttcggcg atctgccgct cgagcgcctg
     2221 gtaggcgccg atgagcagct gctggccggt ctggcctcgg gcgtagaacg tccgcgacac
     2281 ctggacgcca ccgaacgagc ggttgtcgag gagcccgcca tactcgcggg cgaacgggac
     2341 gccctgcgcg acgcactggt cgatgatgtt ggtgctgacc tcggcgaggc ggaagacgtt
     2401 ggactcgcgc gagcggtagt caccaccctt gacggtgtcg tagaagagac ggaagaccga
     2461 gtcgccgtcc tccttgtagt tcttggccgc gttgatgccg ccctgggcgg cgatcgagtg
     2521 ggcgcgccgc ggggagtcct ggtagcagaa ggacttgacc tggtagccgg cctcgccgag
     2581 cgtcgcgcct gccgacgccc cggccaggcc agtgccgacg atgatgacgg acagcttgcg
     2641 ccggttggcc gggttgacca gcttggcctc gaactgcctt gtcgcccagc gatcctcgat
     2701 cggtccgctc ggggccttcg tgtccttgat cggcgcgccc tcgtggtaga gcccgtggac
     2761 gagggtgccg ctggtggtgg tcatctcagt catgaaaggt cgcagccctt acttcacgat
     2821 gccgaacagg atggacagcg gggggagggc gaagcccacg acgacgactg ccgcgatgag
     2881 gcgcgcggcc atcttggcgg cgccccgggc cctgggcgag cccgtccacc ccagggtctg
     2941 cgcgccgctc cagacgccgt gctcgaggtg cagccccagc gcgagcaggg ccaggacgta
     3001 gacgagcacc acccaccaca gctggaagct cgccacgacg agctggtagg ggctgtcacc
     3061 gatcttggcc gcggccacac cggagttgaa gttcaccttg ctgatcgtga actggatgag
     3121 gtggaagacg aggaagagca gcagggccac gccgccccag cgcatcgcct gggaccgcca
     3181 ggccgcggcg ccggccaggc gcacggcata cccggtcggt ctggcggacc tcgcgcgggt
     3241 ccagaggtag aaggccgagt aggcatggcc caccaggctc gcgacgagga cgaggcggaa
     3301 gacccacagg aagccctcgt agggcaggat cggctcgccg agagtccgca ggtggtgcgc
     3361 gtaggtgtcg aaggcgtctt gcccaccgaa gatcttgagg ttgccgtaca tgtgcatcag
     3421 cacatagaag acgaacaacg ccccggtcgt ggccatgagc atcttcatgg cgacggtggt
     3481 ccgacgggcc ttgacctgcg caggggagag ggtcgaggtt gccacgagtg acaccgtacc
     3541 gcgcggccgg aaccgggcca cacccgcgag gggtgtgaag tttccctcgg ctagcctgcc
     3601 tcacgtgagc gaacccatcg acggcttctg ggcggtgatc cccgccgggg gcgccgggac
     3661 ccggctctgg ccgctgtccc gcgccgcgtc cccgaagttc ctgcacgacc tcaccggttc
     3721 tgggcgcacc ttgctccagt cgacgtggga ccggctgacg ccgctcgcgg gtgaccaggt
     3781 cgtcgtggtc accggcgtcg cgcacgccgc cgccgtcgcc ggccagctgc ccggtatgcc
     3841 ggccgctcac ctcctcgtcg agccctcgcc ccgcgactcg atgcccgcca tcgcgctggc
     3901 cgccgcgctg gtcgagcggt gggacccgga cggggtgatc gggtcgttcg cggccgacca
     3961 cgtcatcggt gacgccgtag ccttccatgc ctgcgtccgc gaggcggtgt cggtcgcgcg
     4021 cggcggggcg gtcgtcacac tcggggtcac gccgacgcat ccggccaccg ggttcgggta
     4081 catccgcacg ggggaggcgc tcgccggctt ccccacggcg cgctcggtcg tccggttcgt
     4141 cgagaagcca gacgcagcaa gggcgtcggc gtatgtcgca tccggcgagt tcctgtggaa
     4201 cgccggcata ttcgtcgtgc aggcgagcgt cctgctcgac gtgctcgcgt cataccaccc
     4261 ggatctggcg aggggggccc gcgccatcgc ggccgatctt cggcggctgg gggaggtgtg
     4321 gccgacgatg gccaggat
//
LOCUS       DATAIX010000191         4145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_100109, whole genome shotgun sequence.
ACCESSION   DATAIX010000191 DATAIX010000000
VERSION     DATAIX010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100109"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..992)
                     /locus_tag="VHM65_02885"
     CDS             complement(132..992)
                     /locus_tag="VHM65_02885"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2704676.1"
                     /translation="MSVSTSARVAPAAGSLPVRVGRWFADRFAVIVALFVLAYLFVPV
                     AYTLVFSFNNYKKSNITWNPEGSPTLKHWQNPCGAAGVCESLGVSLQVGVLSTLVATI
                     LGTFIALAMVRYRFVGRGASNMLIFLPMATPEIVLGASLLTIFVQGFNQTFTLGFWTI
                     VIAHIMFCLSFVVVTVKARLQSLDPRLEEAAGDLYAGPVETFWRVTFPLLLPGIVSAA
                     LLSFSLSFDDFIITSFVSGSETTFPKFVYVSYLRGIPAQANVIGFSMFVIAVTLVVVG
                     QVIGNRRKAA"
     gene            complement(989..1903)
                     /locus_tag="VHM65_02890"
     CDS             complement(989..1903)
                     /locus_tag="VHM65_02890"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2704677.1"
                     /translation="MSVMAHAATSAAPVQPGGEDSRKRGLTPYLLLLPGAIWLLIFFV
                     VPLVQLASVSLQSQFPGYPDYYYKDVSFSNYTAAFTEYAGHFGRSMLYAGLATLFGFL
                     LGYPLAYAMAFKVGKWRNIMMICVIAPFFTSFILRTIAWRQILADEGPVVSALRAVHL
                     LPGNGRITETAVAVVAGLTYNFLPFMVLPIYASLERADPRLIEAGGDLYANGLTTFRT
                     VTLPLSMPGVLAGTLLTFIPAAGDYVNAELLGSDKSTKMVGNVIESQFFKVPGGYPTA
                     AALSFTLMALILVLVVLYVRRFGTEELV"
     gene            complement(1900..3051)
                     /locus_tag="VHM65_02895"
     CDS             complement(1900..3051)
                     /locus_tag="VHM65_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2704678.1"
                     /translation="MARNIDALKNAQGDLHVESVTKRFGDFTAVDDLSLTVPRGSFFA
                     LLGPSGCGKTTMLRMIAGLEQPTLGRVLIGHTDLTGSRSYERPVNTVFQNYALFPHLN
                     IRDNVAFAPKRRKIADADTLADETLELVQLSHLATRKPTQLSGGQQQRVAVARAIVNK
                     PEVLLLDEPLGALDLKLRRQMQIELKRIQSEVGLTFIHVTHDQEEAMTMADTVAVMNE
                     GRIEQMGRPEELYDLPRTPFVANFLGQTNLLPVKLVERGEWTLVEGAGRRLRVAPARS
                     IVTEGALALGVRPEKVRIHDSEPDHRGGLTSVIGPGTVIDVSFTGVSTQYLVQVPRMG
                     VWGVFEQNLDVERIDTRPGDQAWLSWAAGHSFVVTGGEALHAGAEQDLT"
     gene            complement(3051..>4145)
                     /locus_tag="VHM65_02900"
     CDS             complement(3051..>4145)
                     /locus_tag="VHM65_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX2704679.1"
                     /translation="PLPPAGGKAALKLPTDVSATDKVVRWANWTAYLDYDEETKKYPT
                     LEEFITKTGIQATYSEDIEDNDAYFSKVSPQLRAGQDIGKDIFTLTDWMANRVIAAQL
                     CQPLELIQMPNAGNLLPALKDVSFDPGRQYSLTWQTGFAGIGYNKAKVGRELKSLDDL
                     WADDLKGRISVLSEFRDTAAMIMQQQGVDISGSFTRTDVETAFAEVSKRVSDGSIRRI
                     RGNSYLEDLKSGNSVAGIVWSGDLFVLRAETEDDNWEFVIPESGGTIWSDNMMVPITS
                     THRRNAEALMNYYYDPQVAAQVAEWVNYICPVQGAQTEMEKIDPDLAKSPFIFPTEAF
                     LKDNNVQAFRALNPQEDQEYSALWAKVVGN"
BASE COUNT          704 a         1296 c         1460 g          685 t
ORIGIN      
        1 gcccgggtga ggtcgtagtc cgcgacaacg agggcctcga agaaatcgcg gtcgacggcg
       61 atcctcgccg ccgcgtcccc gacaccccca gctccgatga tgaggatgcg catgatgccc
      121 tcccgaggcg cctaggccgc cttgcggcgg ttcccgatga cctggccgac gacgacgagg
      181 gtcactgcaa tgacgaacat cgagaagccg atgacgttgg cctgggcggg gatcccgcgc
      241 aggtaggaca catagacgaa cttcgggaac gtcgtctcgc tgccggagac gaagctcgtg
      301 atgatgaagt cgtcaaagct gagcgagaag ctgagcagcg ccgccgacac gatcccggga
      361 agcaggagcg ggaaggtgac ccgccagaag gtctcgaccg gtccggcata caggtccccg
      421 gccgcttctt ctaaccgtgg gtccagggac tgcagccgcg ccttgaccgt gacgacgacg
      481 aagctgaggc agaacatgat gtgggcgatg acgatggtcc agaagcccag cgtgaaggtc
      541 tggttgaagc cctggacgaa gatcgtcagc aggctcgcgc cgaggacgat ctccggggtc
      601 gccatcggca ggaagatgag catgttgctc gcgccgcgac cgacgaagcg gtagcgcacc
      661 atggccaggg cgatgaacgt gccgaggatc gtggccacca gagtcgagag cacgccgacc
      721 tgcaggctga ccccgagtga ctcacagacg cccgccgcgc cgcaggggtt ctgccagtgc
      781 ttgagggtgg ggctgccttc ggggttccac gtgatgttcg acttcttgta gttgttgaac
      841 gagaagacga gggtgtaggc caccggcacg aacaggtacg cgaggacgaa cagcgcgacg
      901 atgaccgcga accggtcggc gaaccaccgg ccgacccgca cgggcaggga tccggccgca
      961 ggcgcgacgc gcgcggaggt cgagacgctc ataccagctc ctccgtcccg aaccggcgca
     1021 catagagcac gacgagcacg aggatgagcg ccatcagcgt gaaggacagc gccgccgccg
     1081 tcggatagcc accgggcacc ttgaagaact ggctctcgat gacgttgccg accatcttgg
     1141 tgctcttgtc cgacccgagc agctcggcgt tgacgtagtc accggctgcc gggatgaagg
     1201 tgagcaacgt cccggcgagg acgcccggca tgctcagcgg cagggtcacc gtccggaacg
     1261 tcgtgagccc gttggcgtag aggtctcctc ccgcctcgat gagccggggg tcggcccgtt
     1321 cgaggctggc gtagatcggc agcaccatga agggcaggaa gttgtaggtc agaccggcga
     1381 cgacggcgac ggccgtctcg gtgatccgcc cattgcccgg cagcaggtgc accgccctga
     1441 gcgccgagac aaccgggccc tcgtcggcga ggatctgccg ccaggcgatc gtgcgcagga
     1501 tgaacgaggt gaagaacggc gcgatgacgc agatcatcat gatgttgcgc cacttgccga
     1561 ccttgaacgc catggcgtag gccagcgggt agcccaggag gaacccgaac agcgtggcca
     1621 gcccggcata gagcatggac cgcccgaaat gccccgcgta ctcggtgaac gccgccgtgt
     1681 agttcgagaa gctgacgtcc ttgtagtagt agtcggggta gcccgggaac tgcgactgca
     1741 ggctcacgct ggcgagctgg acgaggggca cgacgaagaa gatgagcagc cagatcgcgc
     1801 ccggcaggag cagcaggtat ggcgtcagtc cccgcttgcg gctgtcctca cctcccggct
     1861 ggaccggggc ggcgctcgtc gccgcgtgcg ccatcacgct catgtcaggt cctgctcggc
     1921 tccggcgtgc agggcctcgc caccggtgac gacgaaggag tgcccggcgg cccaggagag
     1981 ccaggcctgg tcgcctgggc gcgtgtcgat ccgctcgacg tcgaggttct gctcgaacac
     2041 cccccagacg cccatcctgg ggacctggac gaggtactgg gtcgacaccc ccgtgaacga
     2101 cacgtcgatc accgtgcccg gaccgatgac gctggtcagc ccgccgcggt ggtccggctc
     2161 cgagtcgtgg atgcggacct tttcgggccg gacgccgagc gccaacgccc cctcggtcac
     2221 gatcgaccgg gccggggcga cgcgcaggcg ccgtccggcg ccctcgacaa gggtccactc
     2281 accgcgctcg accagcttga cagggagcag gttggtctgg ccgaggaagt ttgcgacgaa
     2341 cggcgtgcgc ggcaggtcgt agagctcctc gggccggccc atctgctcga tccggccctc
     2401 gttcatcacc gcgacggtgt cggccatggt catggcctcc tcctggtcat gggtgacgtg
     2461 gatgaaggtc aggccgacct cggactggat gcgcttgagc tcgatctgca tctggcgacg
     2521 cagcttgagg tccagcgcac cgagcggttc gtcgagcagg aggacctcgg gcttgttgac
     2581 gatggcccgg gccacagcga cgcgctgctg ctgaccgccg gagagctggg tgggcttgcg
     2641 ggtcgccaga tgcgagagct ggaccagctc gagcgtctcg tcggccagcg tgtccgcgtc
     2701 ggcgatcttg cgtcgtttgg gggcgaaggc gacgttgtcg cggatgttga ggtgcgggaa
     2761 gagggcgtag ttctggaaca cggtgttgac cggacgttcg taagagcgcg agccagtgag
     2821 gtccgtatga ccgatgagca cccggccgag ggtgggctgc tccaggccgg caatcatccg
     2881 cagcatggtg gtcttgccgc acccggaggg gcctagcagg gcgaagaagg agccgcgcgg
     2941 aacggtcagc gagaggtcgt cgacggcggt gaagtcgccg aaccgcttgg tgacggactc
     3001 gacgtgcagg tcaccctggg cgttcttcag ggcatcgatg ttgcgtgcca tcagttcccc
     3061 accacctttg cccacagggc gctgtactcc tggtcctcct gtgggttgag cgcacggaag
     3121 gcttgcacgt tgttgtcctt gaggaaggcc tcggtcggga agatgaacgg gctcttcgcc
     3181 aggtcggggt cgatcttctc catctccgtc tgggcgccct ggaccgggca gatgtagttg
     3241 acccattccg cgacctgagc ggccacctgg gggtcgtagt agtagttcat gagtgcctcg
     3301 gcgttgcgcc ggtgcgtcga ggtgatgggg accatcatgt tgtcgctcca gatcgtgccg
     3361 cccgactcgg ggatgacgaa ctcccagttg tcgtcctcgg tctcggcccg caggacgaac
     3421 aggtcaccgc tccagacgat gcctgcgacg gagttgccgc tcttgaggtc ctcgaggtag
     3481 gagttgccgc ggatccgtcg aatgctgccg tcggacaccc gcttgctcac ctcggcgaag
     3541 gccgtctcga cgtcggtcct ggtgaagctg ccggagatgt ccacaccctg ctgctgcatg
     3601 atcatcgccg cggtgtcgcg gaactccgac aggaccgaga tgcggccctt gaggtcgtct
     3661 gcccacaggt cgtccaggct cttgagctcg cggccgacct tcgccttgtt gtagccgatc
     3721 ccggcgaagc cggtctgcca ggtcagcgag tactgccggc ccgggtcgaa ggagacgtcc
     3781 ttgagggcgg gaagcaggtt gcccgcgttg ggcatctgga tcagctcgag cggctggcac
     3841 agctgggcag cgatgacccg gttggccatc cagtcggtca gcgtgaagat gtccttgccg
     3901 atgtcctgcc cggcccgcag ctggggggac accttcgaga agtacgcgtc gttgtcctcg
     3961 atgtcctcgg agtaggtcgc ctggatcccc gtcttcgtga tgaactcctc gagggtcggg
     4021 tacttcttgg tctcctcgtc gtagtcgagg taggccgtcc agttcgccca gcgcacgacc
     4081 ttgtcggtgg ccgagacgtc cgtggggagc ttgagcgctg ccttcccgcc ggcgggcggc
     4141 agcgg
//
LOCUS       DATAIX010000192         2495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_40701, whole genome shotgun sequence.
ACCESSION   DATAIX010000192 DATAIX010000000
VERSION     DATAIX010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40701"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..901
                     /locus_tag="VHM65_02905"
     CDS             143..901
                     /locus_tag="VHM65_02905"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2704680.1"
                     /translation="MTPLSEALQEARGSLGTVRNAVLLLGLLGEGPAYQHLTDLAERS
                     GLSVPTVHRLLRSLVLADLAEQDPRSSRYGLGPELVRLSHRYLGRLPVLGALAPFLVQ
                     LRDQVGATVHVQGLTRSGIVDLDRVDGSEAGLYRDAHQVRPALSCASGRLLAARGDDE
                     QWKAWLAEAGEPVQELAERDREEWAHADYLVCPGSEAGAPDEVAVPVHDTGAPVPVAL
                     AANLPPATGPAELATVVEHLTRAAHAAARTLGHA"
     gene            894..>2495
                     /locus_tag="VHM65_02910"
     CDS             894..>2495
                     /locus_tag="VHM65_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2704681.1"
                     /translation="MHEQLPHEPDRPAWAAAADLIQWTTRPDSLWEPGDRFGSWFPGG
                     RLNASVACVDRHAERSPDTIAFHWEGEPRDRRSVTYAALLGEVRALTRGLRGLGVGPG
                     DVVALHLGWLPETVVAMFACARIGAAYAVVPQPLPAEALADRLQALAPKVLFTQDGAW
                     RRGTVLPLKARADEALTALDRIEHTVVVRRTGIDVAWFEGDRWYHDLVAGTRPGQGHG
                     DDVPTDVPSCHPLLLVSLANHRGRPVTVTVGTAAMLVQAAALHRWGLAAGRVLWCAGD
                     VSWLGTQVHGIVGPLALGETSVMYEGTLDVPTHARAWKVMRRYDVTTLITTPSVLRTM
                     RSWSFELDRTADAGALRRVVTFGEPGEPALRRWAAEGIGGPRISVADGWGQVELGGIV
                     TVDAPVDPGRMPDAGAKIVDRTGAPVARGQEGELVLTRAWAGAMVGASGAVESIDDVH
                     WRRFPGVYATGDRVRRHKDGRVAFLGRTDQVVSVSGQLVSLREVAEVLLDHPFVQAAD
                     VVERREPSGGRFLLAAVVLSPDRSGE"
BASE COUNT          305 a          933 c          912 g          345 t
ORIGIN      
        1 aaacgcaatg cttgttgtga aactggaatc cgggacgtag agtcgtcgac acggtaggag
       61 cgagggggtt ccggtcggtc gcgggactcg cctggtatga cggcggcgcg cgacgctggt
      121 ccgcaggaag gtgaggcgcc cagtgacgcc gttgagcgag gcgctgcagg aggcccgcgg
      181 cagcctcggc accgtccgca acgccgtcct gctgctcggc ctgctcggcg agggcccggc
      241 ataccagcat ctgaccgacc tcgccgaacg ctcgggcctg tcggtgccga ccgtccaccg
      301 gctgctgcgc tccctcgtgc tggccgacct cgccgagcag gacccgcggt cctcgcgcta
      361 cggccttggc cccgagctcg tccgtctctc ccaccgctac ctcgggcgcc tgccggtgct
      421 cggcgcgctc gcgcccttcc tcgtccagct ccgcgaccag gtcggcgcca cggtgcacgt
      481 ccagggcctg acccggtcgg gcatcgtcga cctcgaccgc gtcgacggct cggaggccgg
      541 cctctaccgc gacgcccacc aggtccggcc ggccctgtcg tgtgccagcg ggcgactcct
      601 cgcggcgcgc ggcgacgacg agcagtggaa ggcgtggctg gccgaggccg gcgagccggt
      661 ccaggagctg gctgagcggg accgggagga gtgggcgcac gcggactatc tggtctgccc
      721 gggctcggaa gccggcgccc cggacgaggt agccgtcccc gtgcacgaca ccggcgcccc
      781 ggtccccgtg gcgctcgcgg ccaacctgcc tcccgccacc ggcccggccg agctggcgac
      841 agtggtcgaa cacctcactc gagcagctca tgcggcggcg cggacgctcg gccatgcatg
      901 agcagctgcc gcacgagccg gaccggccgg cctgggccgc tgcggccgac ctgatccagt
      961 ggacaacccg gccggactca ctctgggagc cgggcgaccg attcgggtcg tggttccccg
     1021 gcgggcggct caacgcctcg gtcgcctgcg tcgaccgtca cgcggaacgc agtcccgaca
     1081 ccatcgcctt ccactgggag ggtgagcccc gggaccgtcg gtcggtgacc tacgccgcgc
     1141 tgctcggcga ggtccgggcc ctgacgcgcg ggttgcgcgg gctcggggtc ggacccggcg
     1201 acgtcgtcgc gctgcacctc ggctggctcc cggagaccgt cgtcgcgatg ttcgcctgcg
     1261 cgcggatcgg ggcggcgtat gccgtcgtcc cccagccgct tccggccgaa gccctcgccg
     1321 accggctcca ggcgctggct cccaaggtcc tgttcaccca ggacggcgcc tggcgacgcg
     1381 gcacggtgct cccgctcaag gctcgtgccg acgaggcgct caccgcgctc gaccggatcg
     1441 agcacacggt cgtcgtgcgc cgcactggca tcgacgtcgc atggttcgaa ggcgaccggt
     1501 ggtaccacga cctcgtggcc ggaacccgcc ctgggcaggg ccacggggac gacgtgccca
     1561 cggacgtgcc ctcctgccac ccgctcctgc tcgtcagcct ggccaaccac cgcgggcgcc
     1621 cggtcaccgt cacggtcggc accgcggcga tgctcgtcca ggccgcagcc ctgcatcgct
     1681 ggggcctggc ggcgggcagg gtgctgtggt gcgccgggga cgtctcgtgg ctcggcaccc
     1741 aggtgcacgg catcgtcggc ccgctcgcgc tcggcgagac cagcgtgatg tacgaaggga
     1801 ccctcgacgt ccccacccac gcgcgagcgt ggaaggtcat gcgcaggtat gacgtcacca
     1861 ccttgatcac gacgccatcg gtcctgcgca cgatgcggtc atggtccttc gagctcgacc
     1921 gcacggccga cgccggggcg ctgcgccggg tcgtcacctt cggcgagccc ggtgagccgg
     1981 ccctgcgccg ctgggccgcc gagggcatcg gtggcccgag gatctcggtc gccgacgggt
     2041 ggggacaggt cgagctcggc gggatcgtca cggtcgacgc gccggtcgat cccgggcgga
     2101 tgcccgacgc cggcgccaag atcgtcgacc gcaccggcgc gcccgtggcc cgtggacagg
     2161 agggcgagct cgtcctgacc agagcctggg cgggcgcgat ggtcggtgcc agcggcgccg
     2221 tcgagagcat cgacgacgtc cactggaggc gcttcccagg ggtctatgcg accggcgacc
     2281 gggtccgccg gcacaaggac ggccgggtcg ccttcctggg ccggacggac caggtggttt
     2341 ccgtctcggg ccagctcgtc tcgctgcgcg aggtcgccga ggtgctgctc gaccacccct
     2401 tcgtccaggc cgccgatgtc gtcgagcggc gcgagccctc gggcgggcgc ttcctgcttg
     2461 ccgcggtcgt gctctcgccg gaccggtccg gcgag
//
LOCUS       DATAIX010000193         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_100152, whole genome shotgun sequence.
ACCESSION   DATAIX010000193 DATAIX010000000
VERSION     DATAIX010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100152"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..>2178
                     /locus_tag="VHM65_02915"
     CDS             144..>2178
                     /locus_tag="VHM65_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX2704682.1"
                     /translation="MSSLFDDLQRPGSGAPGRGPRGANATATASAEVTPAGVPRWAVD
                     GDEPPRAAGARHTGSGVPSVSDPESLLEGLNPQQRAAVVHEGGPLLIIAGAGSGKTRV
                     LAHRIAYLLGARGAQPGQILAITFTNKAAGEMRERVGQLVGSRSTAMWVLTFHSACVR
                     ILRREAAKVGMRSTFSIYDAADSQRLMSMVMRDLDLDPKRYPSRSFSAAVSNLKNELV
                     DDEAFSARVAIDGTHHERMVAEAYRGYQRRLRQANALDFDDLIMTTVHLLQAFPDVAE
                     HYRRRFRHILVDEYQDTNVAQYQLIRELVGFGSDDPHGVPPGELAVVGDADQSIYAFR
                     GATIRNIVEFEQDYPDATTILLEQNYRSTQTILRAANAVIARNASRRPKNLWTDSGDG
                     ARIIGYVADSEHDEAAFVARQIDALGDAHGVRPGDVAVFYRTNAQSRAIEEVLVRVGL
                     PYKVVGGTRFYERREIKDALAYLRVISNPTDTVNLRRILNVPKRGIGDRAVACIASLA
                     ERERIPFVAALGRPEDAPGIATRSVVAVKAFTSLLEDLRTVYDGGAGVCALLEAAVEH
                     SGYLAELRASADPQDETRVENLAELVAVAREYDDAAERDGEPASLETFLEQVSLVADA
                     DQVPDSPEAEAQGVVTLMTLHTAKGLEFPVVFLTGMEDGTFPHLRALGDAHELE"
BASE COUNT          316 a          827 c          739 g          296 t
ORIGIN      
        1 gccctgcggc ggttgggcct cgggcgggtc caggccgtcc gcggcgggtt cgcggcgtgg
       61 cgggccgagg gtctgccggt cgtgctcgcc gactgaccgg gccagccgtg gctgtgggcg
      121 cggccacata ggcttagccc gacatgagca gcctcttcga cgaccttcag cgccctggtt
      181 ccggggctcc cggccggggc ccccgcggcg cgaacgccac cgccacggcg agcgccgagg
      241 tgacccccgc gggcgtgccc aggtgggcgg tcgatggcga cgagcccccg cgagccgcgg
      301 gggcccggca taccggctcg ggggttccct cggtcagcga ccccgagagc ctgctcgagg
      361 ggctcaaccc gcagcagcgc gccgcggtcg tccacgaggg cggtccgctc ctcatcatcg
      421 cgggggccgg gtcgggcaag acccgcgtcc tggcgcaccg catcgcctac ctgctcgggg
      481 cacggggtgc ccagccgggc cagatcctcg cgatcacctt caccaacaag gcggccggcg
      541 agatgcgcga gcgggtcggc cagctcgtcg ggtcgcgcag caccgcgatg tgggtcctga
      601 ccttccactc ggcgtgcgtg cggatcctgc gccgcgaggc ggccaaggtc ggcatgaggt
      661 cgacgttctc gatctacgac gcggccgaca gccagcggct catgtcgatg gtcatgcgcg
      721 acctcgacct cgaccccaag cgctacccct cgcggtcgtt ctccgcggca gtgtcgaacc
      781 tcaagaacga gctcgtcgac gacgaggcgt tttccgcgcg cgtcgccatc gacggcacgc
      841 accacgagcg gatggtggcc gaggcctacc ggggctacca gcgccgcctg cgccaagcca
      901 acgcgctcga cttcgacgac ctcatcatga cgaccgtcca tctgctgcag gcgttcccgg
      961 acgtcgccga gcactaccgg cgacggttcc ggcacatcct cgtcgacgag taccaggaca
     1021 ccaacgtcgc ccagtaccag ctgatccgcg agctcgtcgg cttcggctcg gacgacccgc
     1081 acggggtccc gccgggtgag ctcgccgtcg tcggcgacgc cgaccagtcg atctacgcgt
     1141 tccgcggggc cacgatccgc aacatcgtcg agttcgagca ggactacccc gacgcgacga
     1201 cgatcctgct cgagcagaac taccgctcga cccagacgat cctgcgcgcg gccaacgccg
     1261 tcatcgcccg caacgccagc cgccggccca agaacctgtg gaccgactcc ggcgacggcg
     1321 cacgcatcat cgggtatgtc gcggactccg agcatgacga ggcggcgttc gtcgcccgcc
     1381 agatcgacgc cctcggggac gcccacggcg tgcgccccgg ggacgtcgcg gtgttctacc
     1441 gcaccaacgc ccagtcccgg gcgatcgagg aggtgctcgt ccgggtcggc ctgccctaca
     1501 aggtcgttgg cggcacccgc ttctacgagc ggcgggagat caaggacgcc ctggcctacc
     1561 tgcgggtgat ctccaacccc accgacacgg tcaacctgcg tcgcatcctc aacgtcccca
     1621 agcgcggcat cggtgaccgt gccgtggcct gcatcgcctc gctcgccgag cgcgagcgca
     1681 tccccttcgt cgccgccctc ggccgcccgg aggacgcgcc ggggatcgcg acccgatcgg
     1741 tcgtcgcggt caaggcgttc acctcgctgc tcgaggacct gcgcaccgta tacgacgggg
     1801 gcgccggggt ctgcgctctg ctcgaagccg ccgtcgagca cagcggctac ctcgccgagc
     1861 tgcgcgcgag cgccgacccg caggacgaga cacgggtcga gaacctcgcc gagctcgtcg
     1921 ccgtcgcccg cgagtatgac gacgccgcgg agcgcgacgg cgagccggcc agcctggaga
     1981 ccttcctcga gcaggtctcg ctcgtcgccg atgccgacca ggtcccggac tcacccgagg
     2041 ccgaggcgca gggggtggtc accctcatga cgctgcacac ggccaagggc ctggagttcc
     2101 ccgtcgtctt cctcactggc atggaggacg gcaccttccc gcacctgcgg gccctcggtg
     2161 atgcccacga gctcgaag
//
LOCUS       DATAIX010000194         1959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_70683, whole genome shotgun sequence.
ACCESSION   DATAIX010000194 DATAIX010000000
VERSION     DATAIX010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70683"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VHM65_02920"
     CDS             <1..658
                     /locus_tag="VHM65_02920"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HEX2704683.1"
                     /translation="LAKADQVVVSPNGVKVIGYTDLPARLPTQASQLFGTNLVNLLKL
                     VTPAKDGEATLDMDDVVVRGMTVVHNGTLTWPPPPVQVSAAPAPVAAAPVVAKAATPP
                     SPLRRYGGALVAAALLLLVGWKAPSEFLGHFTVFALAVVVGFYVIRNVHHALHTPLMS
                     VTNAISGIIVVGALLQVGSGDTAATVIAVFAILLASINIFGGFLVTRRMLQMFRRADR
                     "
     gene            655..>1959
                     /gene="pntB"
                     /locus_tag="VHM65_02925"
     CDS             655..>1959
                     /gene="pntB"
                     /locus_tag="VHM65_02925"
                     /inference="COORDINATES: protein motif:HMM:NF006974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - NAD(P)+ transhydrogenase
                     (AB-specific) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEX2704684.1"
                     /translation="MTGVVAATYIVAGLLFVFSLAGLSKHETSKVGNVYGMVGMALAL
                     VATIILAVKNELSAGGLGLIAGAMVIGAGIGIQRARTVEMTAMPELVAMLHSFVGLAA
                     VLVGIDSFLQPGPLAPNMETIHNVEVFIGVFIGAVTFTGSIVAFLKLSARISSKPLML
                     PARHWLNLAAVLVSAGLLGWFIGSHSIVPLLAMTAIALAFGWHLVASIGGGDMPVVVS
                     MLNSYSGWAAAAAGFMLGNDLLIITGALVGSSGAILSYIMCRAMNRSFIAVIAGGFGS
                     DGAVTSGDKDYGEHRETIAEDVADMLNEAQSVIITPGYGMAVAQAQYPVAELTAKLRE
                     RGVTVRFGIHPVAGRLPGHMNVLLAEARVPYDIVLEMDEINEDFSDTDVVLVIGANDT
                     VNPAALEDPGSPIAGMPVLHVWEGKQVIVFKRSMNTGYAGVQN"
BASE COUNT          282 a          714 c          621 g          342 t
ORIGIN      
        1 gctggccaag gccgaccagg tcgtcgtcag ccccaacggg gtcaaggtca tcggctatac
       61 cgacctcccg gcccggcttc cgacgcaggc atcccagctg ttcggcacga acctcgtcaa
      121 cctgctcaag ctcgtcaccc cggccaagga cggcgaggcg accctcgaca tggacgacgt
      181 cgtcgtccgc gggatgaccg tcgtgcacaa cggcaccctc acctggcctc cgccccccgt
      241 tcaggtctcc gcggcaccgg ctccggtcgc cgctgcgccc gtcgtcgcca aggcggcaac
      301 cccgccttca cccctgcgcc ggtatggcgg ggcgctcgtc gctgcggcgt tgctgctgct
      361 cgtcggctgg aaggcgcctt cggagttcct cggccacttc accgtgttcg cgctcgccgt
      421 ggtcgtcggg ttctacgtca tccgcaacgt ccaccacgcg ctgcacaccc cgctcatgtc
      481 ggtgaccaac gcgatctccg gcatcatcgt cgtcggtgca ctgctgcagg tcggctcggg
      541 tgacaccgcg gcgaccgtca tcgccgtgtt cgccatactc ctggcgagca tcaacatctt
      601 cggtggcttc ctcgtcacca gacgaatgct ccagatgttc cggagggcgg accgatgacc
      661 ggggttgttg ccgcgaccta catcgtcgcc gggctgctct tcgtcttcag cctcgcgggg
      721 ctgtccaagc acgagacctc caaggtcggc aacgtctacg gcatggtcgg tatggcgctc
      781 gcgctcgtcg cgacgatcat cctggcggtg aagaacgagc tgtcggccgg cgggctcggt
      841 ctcatcgccg gcgcgatggt catcggcgcg ggcatcggta tccagcgggc ccgcacggtc
      901 gagatgaccg cgatgcccga gctcgtcgcg atgctgcaca gcttcgtcgg tctcgccgcc
      961 gtgctcgtgg gcatcgactc gttcctccag cccgggccgc tcgcacccaa catggagacg
     1021 atccacaacg tcgaggtctt catcggcgtc ttcatcggcg cggtgacctt caccggctcg
     1081 atcgtggcgt tcctcaagct gagcgcccgg atctcgtcca agccgctcat gctcccggcg
     1141 aggcactggc tcaacctggc ggccgtcctc gtcagcgccg gccttctcgg ctggttcatc
     1201 ggctcgcact cgatcgtgcc gctgctcgcg atgacggcca tcgcgctcgc cttcgggtgg
     1261 cacctcgtgg cctcgatcgg cggcggcgac atgcccgtgg tcgtgtcgat gctcaactcc
     1321 tactccggct gggccgcagc cgccgcgggg ttcatgctcg gcaacgacct gctcatcatc
     1381 accggggccc tcgtcgggtc ctccggtgcg atcctgagct acatcatgtg ccgggcgatg
     1441 aaccgctcgt tcatcgcggt catcgccggc gggttcgggt ccgacggggc ggtcacctcg
     1501 ggcgacaagg actacggcga gcaccgcgag acgatcgccg aggacgtcgc ggacatgctc
     1561 aacgaggccc agtcggtcat catcaccccc ggctacggta tggcggtcgc ccaggcccag
     1621 taccccgtcg ccgagctgac ggccaagctg cgcgagcgtg gggtcaccgt ccggttcggg
     1681 atccaccccg tcgccgggcg cctcccggga cacatgaacg tgctgctcgc cgaggcgagg
     1741 gtcccctacg acatcgtcct cgagatggac gagatcaacg aggacttctc cgacaccgac
     1801 gtcgtgctcg tcatcggtgc caacgacacg gtcaacccgg cggcgctcga ggacccgggc
     1861 agcccgatcg ccgggatgcc ggtgctccac gtctgggagg gcaagcaggt catcgtcttc
     1921 aagcgatcga tgaacaccgg gtacgccggc gtccagaac
//
LOCUS       DATAIX010000195         1813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_40741, whole genome shotgun sequence.
ACCESSION   DATAIX010000195 DATAIX010000000
VERSION     DATAIX010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40741"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VHM65_02930"
     CDS             <1..627
                     /locus_tag="VHM65_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX2704685.1"
                     /translation="VEYDAVLGPHRVRAGDVVLALASSGLHSNGYSLVRRVVASAGWA
                     LDRHVEEFGRTLGEELLEPTRVYSADLLALIRADGVAVHALSHVTGGGLAANLARVLP
                     AGLLARVERSRWAPPPVFTTIGRLGHVPVLDLERTLNMGVGFVVVVPADSADAALRAA
                     AARAIPAWVLGEVTTDSPDARGISTEVVQGAKGVDGGAVQVIGSHPGC"
     gene            complement(739..933)
                     /locus_tag="VHM65_02935"
     CDS             complement(739..933)
                     /locus_tag="VHM65_02935"
                     /inference="COORDINATES: protein motif:HMM:NF022717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3073 domain-containing protein"
                     /protein_id="HEX2704686.1"
                     /translation="MGRGRAKAKQTKVARDLKYFSPKTDLSALERELHGSSQRQPDVQ
                     SDSVDDEYNDDYGQWAPGPG"
     gene            complement(1050..>1813)
                     /locus_tag="VHM65_02940"
     CDS             complement(1050..>1813)
                     /locus_tag="VHM65_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase family protein"
                     /protein_id="HEX2704687.1"
                     /translation="REDARVARASPRNGTARCEPGGLADDVHVRDIRADIARGHIAAA
                     QHVWGTPTLAGRRVGIAGVGKVGRLLTAHLLDDGADVVVTDVSPAATHSLTQRFPQVE
                     VAPDAVTLCAMAIDVYSPCALGGALDPATVESLQARIVCGGANNQLAVDGPDGTADRL
                     QERGILYAPDFLVNAGGVIQVSDELSGFDFERAKARTAGIFEATARVLHTAAERGLTP
                     AAAADQLAEERMASAAQRGLRLRDGTGSAAPPFTL"
BASE COUNT          227 a          625 c          678 g          283 t
ORIGIN      
        1 gtcgagtacg acgcggtgct gggtccgcac cgcgtacggg cgggcgacgt cgtgctcgcg
       61 ctcgcgtcga gcggcctgca ctcgaacggc tactcactgg tgcgccgcgt cgtggccagc
      121 gcggggtggg cgctcgaccg ccacgtcgag gagttcggcc ggactctcgg cgaggagctg
      181 ctcgaaccga cccgggtcta ctcggccgac ctgcttgcgc tgatccgcgc cgacggcgtc
      241 gccgtccatg cgctgtcaca cgtgaccggt ggcgggctcg cggccaacct ggcacgggtg
      301 ctgccggccg ggttgctcgc ccgggtcgag cggtcccgct gggccccgcc gccggtgttc
      361 accacgatcg gacgcctcgg ccacgtgccg gtgctcgacc ttgagcgcac cctcaacatg
      421 ggggtcggct tcgtcgtcgt cgtcccggcc gacagcgcag acgcggcgct gcgcgcggcg
      481 gccgcgcgcg ccataccggc atgggtgctc ggcgaggtga ccacggactc gccggacgcc
      541 cgtggcatct cgaccgaggt cgtgcagggt gccaagggtg tcgacggcgg agccgtccag
      601 gtcatcggga gccacccggg gtgctaggcg cgcgggtcgt cgacctcggc gctgcacgat
      661 ctcgccgacc ttcggcgcgc gcacgaactc gcgccgacct gcggcgcgag cgatgagggg
      721 tcgaaagcgt cgggcggctc agcctgggcc cggcgcccac tgaccgtagt cgtcgttgta
      781 ctcgtcatcg acggagtcag actggacgtc tggctgacgt tgtgacgaac cgtgcagttc
      841 tcgctcaagc gcgctcaagt cggtcttcgg ggagaagtac ttcaagtccc gagcgacctt
      901 ggtctgcttg gctttggccc ggccgcgccc catggcgtga ccccctcatg cgtctgccgg
      961 ggcggcatca ctcgggttcg ttggggcgtc acgcccttcg agccgtcgcg gagcggtccc
     1021 gggaaatgtt cgtcttcgtg gggaccacgc tacagggtga aagggggtgc tgccgagccc
     1081 gtcccgtcac ggagcctgag gccgcgctgc gcggcgctcg ccatccgctc ctcggcgagc
     1141 tggtcggccg ccgctgcagg tgtcagccca cgctcggctg cggtgtgcag gacccgcgcc
     1201 gtggcctcga agatgcctgc cgtccgggcc ttggcccgct cgaagtcgaa gccggacagc
     1261 tcgtcgctga cctggatcac gccaccggcg ttgacgagga agtctggggc gtagaggatt
     1321 ccgcgctcct ggagccggtc ggcggtgccg tccggcccgt cgacggcgag ctggttgttg
     1381 gcgccgccgc agacgatccg ggcttggagg gactccaccg tggcggggtc gagagcgccg
     1441 ccgagcgcgc acggtgagta cacgtcgata gccatggcgc acaacgtcac cgcgtccggc
     1501 gccacctcga cctgggggaa ccgctgggtc agcgaatgcg tcgcggcggg gctgacgtcg
     1561 gtgacgacga catcggctcc gtcgtcgagc aggtgtgcgg tcagcaggcg gccgaccttg
     1621 cccacgccgg cgatcccgac ccggcgaccg gcgagggtgg gtgtccccca cacgtgctgg
     1681 gcggccgcta tgtgaccgcg tgcgatgtcg gcacgtatgt cgcggacatg gacgtcatcg
     1741 gcgaggccac caggttcgca acgggccgtt ccgttgcgcg gggaggcgcg ggcgactcga
     1801 gcgtcctcac ggc
//
LOCUS       DATAIX010000196         2762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_40795, whole genome shotgun sequence.
ACCESSION   DATAIX010000196 DATAIX010000000
VERSION     DATAIX010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40795"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VHM65_02945"
     CDS             complement(<1..462)
                     /locus_tag="VHM65_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704688.1"
                     /translation="MSLRRPRLKSYFRPLERGAGALQLGLDPQAGAVVLEGLSEPEIA
                     FVCGLDGTRSASRVYADASAAGIAPDRTARLLALLRDHGLLVRDQAGRGRLGALPDPL
                     RQALLADAQAFGLVYDGLDDGFHVLAARRRQSVLVGGAGALPWSVATILRAS"
     gene            complement(624..785)
                     /locus_tag="VHM65_02950"
     CDS             complement(624..785)
                     /locus_tag="VHM65_02950"
                     /inference="COORDINATES: protein motif:HMM:NF039193.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEX2704689.1"
                     /translation="MAAETYKGEFYCVKCKAKKEAEGKVVVSENGRRMAKGQCPTCGT
                     NLNRILGKA"
     gene            976..1353
                     /locus_tag="VHM65_02955"
     CDS             976..1353
                     /locus_tag="VHM65_02955"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF010997.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX2704690.1"
                     /translation="MGEHRTGRTGRIASSPDAATAGGLGSRAGGAVPVRVTVFVQGGV
                     QGVGFRWWTRSRALELGLEGFARNLADGRVEVCAQGRPEAVDRLVVLLGEQPSTRRRP
                     GWVDSTTILWASPREDLTGFAEW"
     gene            1395..2210
                     /locus_tag="VHM65_02960"
     CDS             1395..2210
                     /locus_tag="VHM65_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX2704691.1"
                     /translation="MSWWEQDYEDLRVERVEHGPRVVAVLTLDQPDKRNAMSTAMTAS
                     WVRAIAALRADDALAAVVVTGTGQAFSAGGDLSWIVSEPDASVVSLRSRMLAFYRDWL
                     SIGTLEVPTIAAVNGHAVGAGFALALACDIRYAAADAKLAVPFTSLGLHPGMATTWSL
                     PNVAGAAVARDLLLTGRVVTGEEAMTLGLVSRSLPRDAVLPAAMAAARQVAASAPIAT
                     RLTVQALRGGGHATCDDAIHWEALAQAVTLATADVHEGIAAAAQRRVPGFTGR"
     gene            2217..>2762
                     /locus_tag="VHM65_02965"
     CDS             2217..>2762
                     /locus_tag="VHM65_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX2704692.1"
                     /translation="MATTEVEVRRSARRRRTVSAYREGGKTIVLIPARFTAADERRWV
                     DVMVARIERSESRRRPSDAVLAARAAELSSEYLRGGARAASVRWAANQRTRWGSCTPS
                     DGSIRISTRVQGMPGYVLDYVLLHELAHLLEAGHGERFWTLLAAYPRLERARGYLEGV
                     AAASGLDVSDDGPGRKVGHSGE"
BASE COUNT          365 a          981 c         1024 g          392 t
ORIGIN      
        1 gctcgcccgc aggatcgtcg cgaccgacca gggcagcgcc ccggccccgc cgacgagcac
       61 gctctgccgc cgtcgcgccg cgaggacgtg gaacccgtcg tcgagcccgt catagacgag
      121 accgaacgcc tgggcgtcgg cgaggagggc ctggcgcagt gggtcgggga gagcgccgag
      181 cctgccacgt ccggcctggt cccggacgag gaggccatgg tcgcgcagga gggccagcag
      241 ccgggcagtc cggtcgggcg caatccccgc cgcgctcgcg tcggcataca cccggctcgc
      301 ggaccgcgtg ccgtccagac cgcagacgaa ggcgatctcg ggctcgctca gaccctcgag
      361 gacaaccgct ccggcctgcg ggtccaggcc gagctggagc gcgcccgcgc cgcgctcgag
      421 cggacggaag tacgacttga ggcgaggccg gcgcagggac atcgggacct tccggtggcg
      481 tggtccctcg actgtgccag accacccggg tcaggccgtc ccgtcatcca caggcttcgg
      541 ctccctgcgg cgcaggacaa cgcgagagcg gggtcgcgcc ggatggcgaa ccccgctctc
      601 gcgttggtcg atcgtggcgg cgctcaggcc ttgccgagga tccggttcag gttcgtcccg
      661 cacgtcgggc actggccctt ggccatccgg cgaccgttct cgctgacgac aaccttgcct
      721 tcggcttcct tcttggcctt gcacttcacg cagtagaact cgcccttata ggtctccgct
      781 gccatgtggc tctccactcc ttcgtctggg tcccgccggc ccggcggact tgtccggcac
      841 cgaacccggc tcaaccctag accagcgcac gctcagaacc gcatattgtc cgcggcgggc
      901 cgcgcatggg ttccgccgcc actgcaccgg gcgggatccc ggcggatgtc ggtcgcccgg
      961 cctacgatca cggatgtggg agaacaccgc accggccgca ccggccgcat cgcgtccagt
     1021 cctgacgcgg ccaccgccgg cggcctcggc agccgagccg ggggcgccgt gcccgtgcgc
     1081 gtcacggtgt tcgtccaagg tggcgtccag ggcgtcggat tccggtggtg gacacgctca
     1141 cgcgccctcg agctcgggct cgaggggttc gcccgcaacc tcgccgatgg tcgggtcgag
     1201 gtgtgtgccc agggccggcc ggaggccgtc gaccgactcg tggtgctgct cggagagcag
     1261 ccgagcacgc gtcggcggcc tgggtgggtc gactcgacga cgatcctgtg ggcgtcgccc
     1321 cgcgaggatc tcacgggctt cgccgagtgg tgaggaacgg acgtggacag gacacaacga
     1381 cggcaggagc ggtgatgagc tggtgggagc aggactatga ggacctgcgc gtggagcgcg
     1441 tggagcatgg gccacgggtg gtggcggtcc tgaccctcga ccagccggac aagcgcaacg
     1501 cgatgagcac ggccatgacc gccagctggg tccgggccat cgctgcgctg cgtgcggacg
     1561 acgcgctggc cgcggtcgtc gtgaccggta ccgggcaggc gttcagcgcg gggggagacc
     1621 tgtcctggat cgtctccgag cctgacgcca gcgtcgtctc gctgcgctcg cggatgctcg
     1681 ccttctaccg ggactggctc tccatcggga ccctcgaggt gccgacgatc gccgcggtca
     1741 acgggcatgc cgtcggcgcc gggttcgcgc tcgcgctggc ctgcgacatc cggtatgccg
     1801 ccgctgacgc caagctcgca gtccccttca cctcgttggg actgcatccc gggatggcga
     1861 cgacgtggtc cctgcccaac gtcgcgggcg ccgcggtggc gcgggacctg ctcctgaccg
     1921 ggcgcgtcgt gaccggggag gaggcgatga cgctcggcct cgtctcgcgc tccctgcccc
     1981 gggatgcggt gcttcccgcg gccatggcgg ccgcccggca ggtggcggcc tcggctccca
     2041 ttgcgaccag gctcaccgtc caggcgctgc ggggcggcgg gcacgcgacc tgcgacgacg
     2101 cgatccactg ggaggcgctc gcgcaggcgg tcacgctcgc caccgcggac gtgcacgagg
     2161 gcattgccgc tgccgcgcaa cgccgcgtcc cgggcttcac ggggcgctga gtgccggtgg
     2221 ccaccactga ggtcgaggtc cgccgcagcg ctcggcggcg ccgcacggtc agtgcctacc
     2281 gtgagggcgg caagacgatc gtgctcatcc cggcgcggtt cacggccgcc gacgagcggc
     2341 gctgggtcga tgtcatggtg gccaggatcg aacgctcgga gagccgtcga cggcccagcg
     2401 atgcggtgct ggccgcgcgg gcggccgagc tgtcgagcga gtacctcagg ggtggggccc
     2461 gggcggcgag cgtgcgctgg gccgccaacc aacgcacccg gtgggggtcg tgcacgccct
     2521 cggacgggtc gatccggatc tcgacgaggg tccaggggat gcccggctat gtcctggact
     2581 atgtcctgct ccacgagctg gcgcacctgc tcgaagccgg ccatggggag cggttctgga
     2641 ccctgctcgc ggcctacccc cggctcgagc gcgcacgagg ctatctcgaa ggggtggccg
     2701 ctgcgtcagg tctcgacgtc agcgacgacg gacctggccg caaggtcggc cacagcggcg
     2761 ag
//
LOCUS       DATAIX010000197         1881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_70791, whole genome shotgun sequence.
ACCESSION   DATAIX010000197 DATAIX010000000
VERSION     DATAIX010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70791"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..1424)
                     /locus_tag="VHM65_02970"
     CDS             complement(225..1424)
                     /locus_tag="VHM65_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2704693.1"
                     /translation="MARLQQTEGLTEEQGELVKLVRQFVDEQIIPVATALEHKDEYPA
                     EIVEGMKEMGIFGLMIPEEYGGLGESLLTYALCVEEIARGWMSVSGIINTHFIVAYML
                     IQHGTDEQKQRYLPRMATGEVRGAFSMSEPGCGSDVSAIKSKAVQGPGDEWVVNGQKM
                     WLTNGGSANLVAVLVKTDTGANSVYKNMTTFLIDKESGFGQTAQGITVPGKIEKMGYK
                     GVDTTELVMEGHRTTSAQVLGGVPGKGFYQMMDGVEVGRVNVAARACGLAMRAFELGI
                     AYSQQRETFGKKIAEHQAVLFRLAEMATKCEAAHQMMVKAARKKDSGERNDLEAGMAK
                     YLASEFCRDVVEDSFRIHGGYGYSKEYEIERLYREAPMLLIGEGTADIQRMIIGRRLL
                     EDYALKG"
BASE COUNT          276 a          658 c          600 g          347 t
ORIGIN      
        1 gttctggacc gggaaatcct tgtcgctcag ggtgtcgacg gccttctgcg ccgcggtata
       61 ggtgtcgaag acgccgagcg acatggggta ctcgagggtg agaacgggga atcggccacg
      121 cggggtgacg gatggcatgc tcatagcggt catggtctca cctcagcctg gacgcccgct
      181 ggacgccccc tgtgtgctcg gccccggcgt gcccgcgggc acgctcagcc cttgagtgcg
      241 tagtcctcca ggagacgccg gccgatgatc atccgctgga tgtcggccgt cccctcgccg
      301 atgagcagca tcggcgcctc acggtagagc cgctcgatct cgtactcctt ggagtagccg
      361 tacccgccgt ggatgcggaa cgagtcctcg acgacgtcgc ggcagaactc ggaggcgagg
      421 tacttcgcca taccggcctc gaggtcgttg cgctcgcccg agtccttctt gcgcgcagcc
      481 ttgaccatca tctggtgggc ggcctcacac ttggtcgcca tctcggcgag gcggaacagc
      541 acggcctggt gctcggcgat cttcttgccg aaggtctcgc gctgctggct gtaggcgatg
      601 ccgagctcga acgcccgcat cgccaggccg cacgcgcgag ccgcgacgtt gacccggccg
      661 acctcgacgc cgtccatcat ctggtagaag cccttgccgg ggacgccgcc caggacctgg
      721 gccgacgtcg tgcggtgccc ctccatgacg agctcggtcg tgtcgacgcc cttgtagccc
      781 atcttctcga tcttgcccgg cacggtgatg ccctgggcgg tctggccgaa gccggactcc
      841 ttgtcgatga ggaaggtcgt catgttcttg tagacgctgt tcgcgccggt gtcggtcttg
      901 accaggacgg cgacgaggtt ggccgagccg ccgttggtca gccacatctt ctgcccgttg
      961 acgacccact cgtcacccgg gccctggacc gccttggact tgatcgcact gacatccgag
     1021 ccgcagccgg gctcgctcat cgagaaggcg ccccggacct caccggtcgc catccgcggc
     1081 aggtagcgct gcttctgctc gtcggtgccg tgctggatca gcatgtaggc gacgatgaag
     1141 tgcgtgttga tgatcccgct gacgctcatc cacccccgcg cgatctcctc gacgcacagg
     1201 gcataggtca gcagcgactc gcccagcccg ccatactcct ccgggatcat gagcccgaag
     1261 atccccatct ccttcatgcc ctcgacgatc tcggcggggt actcgtcctt gtgctcgagc
     1321 gcggtcgcga ccgggatgat ctgctcgtca acgaactggc gcaccagctt gaccagctcg
     1381 ccctgctcct cggtcagtcc ttcggtctgc tgcagtcgcg ccatggggga tcaccttacg
     1441 tccgcggatg gttgaggttc ctctcgcgag tatgccgagt gcccggctcc cagcgacacc
     1501 cacctcgctg tgagtcccac cacccggtcg cgtcctcccc cgcgtcgagg tcgggcaatg
     1561 tcggctcatc tcacggtgtg gggcggtgaa gggcggtggg ggaaaccggt tgccacgggt
     1621 gctggggagc ctggacactt gagccagcgc gcgccatacg acgcgggtgt tccgacggac
     1681 tccgaggagg gtgacatgca gaccacggcc ctgggtgcct catgcaaccc gaccccctcc
     1741 accaggaagt cctgccttga cctcgttcac cgaacacgag ctcttctcct ctgactctca
     1801 ccccgacggc ccgcccgaag gcgagcgctg gtcgtcctgg gacggtgcca tgcacggtcc
     1861 caccccccgg cccgactggg t
//
LOCUS       DATAIX010000198         3058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_40825, whole genome shotgun sequence.
ACCESSION   DATAIX010000198 DATAIX010000000
VERSION     DATAIX010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40825"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /gene="deoC"
                     /locus_tag="VHM65_02975"
     CDS             <1..594
                     /gene="deoC"
                     /locus_tag="VHM65_02975"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX2704694.1"
                     /translation="VAAVATAFPSGRASMTVKLADTRDAIEAGADEIDMVIDRGAFLS
                     GRYRQVFDEIAAVKAACGSTHLKVIMETGELVTYDNVRRASWLCMLAGADFIKTSTGK
                     VAPAATLPVTLIMLEAVRDWRDLTGEMVGVKPAGGIRTSKDATKYLVTVNEVAGEDWL
                     SSTWFRFGASSLLNDLLLQRQRLRLGAYSGPDYVTID"
     gene            606..2066
                     /locus_tag="VHM65_02980"
     CDS             606..2066
                     /locus_tag="VHM65_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2704695.1"
                     /translation="MSRFGYAPAPESRSVVDIASSYGLFIDGVMTDGSGSTSFKTVNP
                     ATEEVLAEVTEASAADVDRAVAAARRAYEGVWGRMSGADRGKYLFRIARLMQERAREF
                     AVLETLDNGKPIKESRDVDVPLAAAHFFYHAGWADKLEYAGLAPAGSAPPRPVGVVGQ
                     IIPWNFPLLMLAWKIAPALACGNTVVLKPAETTPLTALLFAQVCQQADLPAGVVNIVT
                     GAGDTGRALVEHAGLDKIAFTGSTEVGKSIARAVAGTRTRVTLELGGKAANIIFDDAA
                     IDEAVEGVVNGIFFNQGHVCCAGSRLLVQESVHDEVVRKLQRRLGTIRVGDPMDKNTD
                     LGAINSATQLARVRELSDAGEAEGAVRWSPACELPAKGFWFPPTIFTGVAQTHRIAQE
                     EVFGPVLSVLTFRTPQEAVAKANNTPYGLSAGIWTEKGSRILWMADHLRAGVIWANTF
                     NVFDPASPFGGYKESGYGREGGRHGLEAYVTTGAAR"
     gene            2063..2971
                     /locus_tag="VHM65_02985"
     CDS             2063..2971
                     /locus_tag="VHM65_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2704696.1"
                     /translation="MSPTPAARVDVRKTYKLYIGGTFPRSESGRSYAVYGASRTRPFL
                     ANAAQGSRKDARDAVVAARAAAPGWAGATAYNRGQVLYRVAEVMTGRHAQLTKEVAVG
                     EGLAPAKASALVDQAIDRWVWYAGWTDKLAQVLGGLNPVAGSFFNISAPEPTGVVAAL
                     APQRSSLLGLVSVLAPIIVSGNTAVVIASHERPLPAVTLGEVLATSDVPAGVVNLVTG
                     DPAEIAPWLASHRDVNAIDLTGAADAAGVDWAALEAAAADSVTRVLRPAGSGAEAVEP
                     DFGLAPGLDRVRAFLETKTVWHPKGR"
BASE COUNT          453 a         1127 c         1043 g          435 t
ORIGIN      
        1 gtcgccgctg tcgcgaccgc cttcccgtca ggacgcgcga gtatgacggt caagctcgcc
       61 gacacgcgcg acgccatcga ggccggggcc gacgagatcg acatggtcat cgaccgcggg
      121 gcgttcctgt ccggtcgcta ccgtcaggtc ttcgacgaga tcgcggccgt caaggcggcc
      181 tgcggctcga ctcacctcaa ggtgatcatg gagaccggcg agctcgtcac ctacgacaat
      241 gtccgccgag cctcgtggct gtgcatgctc gcgggagccg acttcatcaa gacctcgacc
      301 ggcaaggtcg caccggccgc caccttgcct gtcacgctca tcatgctcga agcggtccgc
      361 gactggcgcg acctcaccgg cgagatggtc ggggtgaagc ccgccggtgg catccgcacg
      421 agcaaggacg cgacgaagta cctcgtcacc gtcaacgagg tcgccggtga ggactggctc
      481 tcttcgacgt ggttccgctt cggtgcgtcg agcctgctca acgacctgct cctgcaacgc
      541 cagaggctgc gcctcggcgc ctactcgggc cccgactacg tcacgatcga ctgaaggacc
      601 acggcatgag caggttcggc tacgcccccg cccctgagtc gcgctcggtc gtcgacatcg
      661 cctcgtccta cgggctgttc atcgacggcg tgatgaccga cggctccggg agcacctcct
      721 tcaagacggt caacccggcc accgaggagg ttctcgccga ggtcaccgaa gccagtgccg
      781 ccgacgtcga ccgcgccgtg gcggccgcgc gccgcgccta cgagggggtg tggggccgga
      841 tgtccggcgc cgaccgcggc aagtaccttt tccggatcgc ccggctcatg caggaacgcg
      901 cgcgggagtt cgccgtcctg gagaccctcg acaacggcaa gccgatcaag gagagccgcg
      961 acgtcgacgt cccgctcgcc gcggcgcact tcttctacca cgcgggctgg gccgacaagc
     1021 tcgagtatgc cgggctggcg ccggccggca gcgcgccacc ccgcccggtc ggtgtcgtcg
     1081 gccagatcat cccctggaac ttcccgctgc tcatgctggc ctggaagatc gctccggcgc
     1141 tggcctgtgg caacaccgtc gtcctcaagc cggccgagac gacgccactg acggcgctgc
     1201 tcttcgcgca ggtctgccag caggccgacc tgccggcggg cgtcgtcaac atcgtcaccg
     1261 gcgctggaga caccggtcgc gccctcgtcg agcacgccgg gctggacaag atcgccttca
     1321 ccgggtcgac cgaggtcggc aagtcgatcg cccgcgccgt ggcggggacc cgcacgcgcg
     1381 tcactctcga gctcggcggc aaggccgcca acatcatctt cgacgacgcg gcgatcgacg
     1441 aggccgtcga gggcgtcgtc aacgggatct tcttcaacca gggccacgtg tgctgcgcgg
     1501 ggtcgcgcct gctcgtgcag gagtccgtcc acgacgaggt cgtccgcaag ctgcagcgac
     1561 gcctgggcac catccgggtc ggtgacccga tggacaagaa caccgacctc ggggcgatca
     1621 actcggccac ccagctggcg cgggtgcgcg agctctcgga tgccggtgag gccgaggggg
     1681 cagtgcgctg gagcccggcc tgcgagctcc cagccaaggg cttctggttc cccccgacga
     1741 tcttcacggg agtcgcccag acccaccgga tcgcccagga ggaggtcttc ggcccggtcc
     1801 tgtccgtcct caccttccgc accccccagg aggcggtggc gaaggccaac aacacgccct
     1861 acgggctctc ggccggcatc tggaccgaga agggctcgcg catcctctgg atggccgacc
     1921 acctgcgcgc gggggtgatc tgggccaaca cgttcaacgt gttcgacccg gcctcgccgt
     1981 tcggcggcta caaggagtcg ggctacggcc gcgagggcgg tcgccacgga ctcgaggcct
     2041 acgtcacgac gggagcagcc cgatgagccc gaccccggcc gcgcgggtcg acgtgcgcaa
     2101 gacctacaag ctctacatcg gcggcacgtt cccccgcagc gagtccggtc gctcgtatgc
     2161 cgtgtacggc gcctcgcgca cccggccctt cctcgccaac gccgcccagg gctcgcgcaa
     2221 ggatgcccgc gatgccgtcg tggccgcccg ggccgcagcc ccgggctggg cgggggcgac
     2281 ggcatacaac cgcgggcagg tgctctaccg cgtcgccgag gtcatgacgg gccggcatgc
     2341 ccagctcacc aaggaggttg cggtcggcga agggctggct ccagccaagg cgtcggcgct
     2401 cgtcgaccag gccatcgacc gctgggtgtg gtacgccggg tggaccgaca agctcgcgca
     2461 ggtcctcggc gggctcaacc cggtcgccgg cagctttttc aacatctcgg ccccagagcc
     2521 gaccggggtc gtcgcggccc tggcgcccca gcggtcctcc ctgctcgggc tggtcagcgt
     2581 cctggccccg atcatcgtca gcggcaatac cgccgtcgtc atcgccagcc acgagcggcc
     2641 actgcccgcg gtcaccctag gcgaggttct tgcgacgagc gacgtgcccg ctggcgtggt
     2701 caacctcgtg accggcgacc cggccgagat cgcgccctgg ctcgcctcgc accgcgacgt
     2761 caacgcgatc gacctcaccg gggcggcgga cgccgccggt gtcgactggg ctgccctgga
     2821 ggccgcggcg gcggacagcg tcacgcgagt gcttcgcccc gccgggtccg gtgccgaggc
     2881 ggtcgagccg gacttcggtc tcgcccccgg cctggaccgg gtccgcgcgt tcctcgagac
     2941 gaagaccgtc tggcacccca agggacgctg acgaggcagc gcaggcgcca ctgctgggcc
     3001 acgcgtcgcg gaggcaccac cggacacgag gtcagtgcgc gcgctgccgc tggtagcg
//
LOCUS       DATAIX010000199         2361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_100522, whole genome shotgun sequence.
ACCESSION   DATAIX010000199 DATAIX010000000
VERSION     DATAIX010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100522"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /gene="recF"
                     /locus_tag="VHM65_02990"
     CDS             complement(<1..1163)
                     /gene="recF"
                     /locus_tag="VHM65_02990"
                     /inference="COORDINATES: protein motif:HMM:NF000822.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX2704697.1"
                     /translation="MYVRHLQVGDFRSYVTADLALSPGVTTLLGLNGQGKTNLVEAIG
                     YVASLSSHRVATDQPLVRFGADRATIRAAVCRHDRETLVELELTPGRANRAKLNRAAA
                     ARPRDVLGVLRLVLFAPEDLALVKGDPSERRRFLDDLLVARQPRWAGVRSEYDRVLRQ
                     RNALLKSARPVLRPGPGRRPRPGDPALEEARTSALHTLDVWNTQLAQVGAQLLYARLR
                     LMRDLAPDLATAYSEVSASASEARMSYRASLREDAATRIAAGDVPAPEWLNDELLATF
                     DQVRGAEIDRGVSLAGPHRDDVVLSLGDLPAKGYASHGESWSLALGLKLAAYELLRRD
                     LGEDPVLILDDVFAELDVGRRNRLAERVRDCEQVIITAAVPADVPPPLAASGMT"
     gene            complement(1214..2134)
                     /gene="gnd"
                     /locus_tag="VHM65_02995"
     CDS             complement(1214..2134)
                     /gene="gnd"
                     /locus_tag="VHM65_02995"
                     /inference="COORDINATES: protein motif:HMM:NF007161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX2704698.1"
                     /translation="MQLGLIGLGKMGGNMRERIRRAGHEVVGYDRNPEVSDVRGMTAL
                     VAALQAPRVVWVMVPSGDPTRDTVRRLGELLSPGDLVIDGGNSRFTDDFDNAKLLKAK
                     GIGYLDCGVSGGIWGLDNGYGLMVGGETTWVRAAMPIFDALRPEGPRDEGFVHAGEVG
                     AGHYAKMVHNGIEYGLMAAYAEGYELLEAKDIVTNVPGAFKAWTRGTVVRSWLLDLLV
                     KALEDDPSLAEVSGYTADSGEGRWTVEEAIANSVPMPVISASLFARFASRQDESPTMK
                     AVAALRGQFGGHAVMSVAEGRSLRSEGAPS"
BASE COUNT          361 a          798 c          866 g          336 t
ORIGIN      
        1 gtcatcccgg aggcagcgag aggtggtggg acatcggcgg ggacagcggc ggtgatgatg
       61 acctgttcgc agtccctgac ccgttcggcg aggcggttgc gccgcccgac gtcgagctcg
      121 gcgaagacgt cgtcgaggat cagcaccgga tcctcgccga ggtcacgacg aaggagctca
      181 taggccgcga gcttgagtcc cagcgcgagg gaccaggact ccccgtggct ggcgtagccc
      241 ttggccggga ggtctcccag ggacaggacc acgtcgtcgc ggtgtggtcc ggccagtgac
      301 acacctcggt cgatctcggc gccgcggacc tggtcgaagg tggccagcag ctcgtcgttg
      361 agccactctg gagcagggac gtcgccggcc gctatccgcg tcgcggcgtc ctcgcgcagg
      421 gaggcacggt aggacatccg ggcctcgctc gcgctcgcac tgacctcgga gtatgccgtc
      481 gccaggtccg gtgcgaggtc gcgcatgagc cggagccggg catagagcag ctgggcgccg
      541 acctgggcca gctgggtgtt ccagacgtcg agggtatgca gcgccgaggt ccgtgcctcc
      601 tcgagcgcgg ggtcccccgg ccgggggcgc cgcccggggc ccgggcgcag gaccggcctg
      661 gccgacttga gcagcgcgtt gcgttgcctg agcacccggt catactccga tcggacgccc
      721 gcccagcgcg gctggcgggc cacgagcagg tcatcgagga agcgacgccg ctccgagggg
      781 tcgcccttga ccagcgccag gtcctcgggc gcgaagagca cgaggcgcag gacgccgagg
      841 acgtcgcggg gacgcgccgc ggcggcccgg ttgagcttgg cccggttggc ccggcccggt
      901 gtcagctcga gctcgacgag ggtctcgcgg tcatgccggc agacggccgc ccggatcgtg
      961 gcccggtcgg caccgaagcg caccagcggc tggtcggtgg ccacccggtg cgaggacaac
     1021 gaggcgacgt agccgatcgc ctcgacgagg ttcgtcttgc cctggccgtt gagcccgagc
     1081 agggtcgtga cgccagggga cagagcgaga tcggccgtga cgtagctgcg gaagtcacca
     1141 acctgcagat ggcggacgta cacgtgtcag cgctccaacg acccactgag ggcgcagagc
     1201 agcgggatgc tgctcagctc ggagccccct cggaccgcag cgacctgccc tcggccacgc
     1261 tcatcacggc atgcccgccg aactgtccgc gcagcgcggc caccgccttc atcgtcggcg
     1321 actcgtcctg ccgggaggcg aagcgggcga agagcgaggc cgagatcacc ggcatcggca
     1381 cggagttggc gatggcctcc tcgaccgtcc agcggccttc gccggagtcg gcggtgtagc
     1441 cggagacttc agcgaggctg gggtcatcct cgagggcctt gacgagcagg tcgagcaacc
     1501 aggagcgcac gaccgtgccg cgggtccatg ccttgaaggc accgggcacg ttggtgacga
     1561 tgtccttggc ctccagcagc tcgtagccct cggcataggc cgccatgagg ccgtactcga
     1621 tcccgttgtg gaccatcttg gcgtagtgcc ccgcgcccac ctcgccggcg tggacgaagc
     1681 cctcgtcgcg ggggccctca ggacgcagcg catcgaagat cggcatcgcc gcgcggaccc
     1741 acgtcgtctc gccgccgacc atgagcccgt agccgttgtc gagcccccat atcccaccgg
     1801 acacgccaca gtcgagatag ccgattcctt ttgccttcaa caattttgcg ttgtcgaagt
     1861 cgtccgtgaa gcgggagttc ccgccatcga tgacgaggtc cccgggggag agcagctcgc
     1921 cgagccggcg cacggtgtcc cgagtggggt cgccggacgg caccatgacc cacacgacgc
     1981 ggggggcctg cagcgccgcg acgagggccg tcataccgcg gacgtccgag acctcggggt
     2041 tgcggtcgta gcccacgacc tcgtgccccg cgcgccggat ccgctcgcgc atgttgccac
     2101 ccatcttgcc caagccgatc agtccgagct gcatgatcgc gtgccttccg tggtgtggcg
     2161 tcaggggtcg caggcgggct cggccggcct agctcgcgaa gcgaaccggc atcaggacgt
     2221 agcgataggc caggtcgggg tcggcatcgg gatcggcctg accggccatg agcgccgggc
     2281 ggccggtctg ggtgaacgac agacgggtgt atggcgtccc gagggcgtgc agtccgtcga
     2341 gcaggaagtg cgggttgaag g
//
LOCUS       DATAIX010000200         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_100621, whole genome shotgun sequence.
ACCESSION   DATAIX010000200 DATAIX010000000
VERSION     DATAIX010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100621"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VHM65_03000"
     CDS             <1..182
                     /locus_tag="VHM65_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704699.1"
                     /translation="LTASHQRVVAVAVVIADGVLRDRIRVAADGAGVELVPGVLAHTG
                     ALANLVSLRAGNLEG"
     gene            179..1003
                     /locus_tag="VHM65_03005"
     CDS             179..1003
                     /locus_tag="VHM65_03005"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX2704700.1"
                     /translation="MTASEPTIPTQNPADQSSPHPALRVTRTPDLVTVTLCDPRTRNA
                     QTPSLWLALAEVARSIGPGVRVVIIDAEGESFSAGLHRGMLSPGGLPGEPPVLALAAG
                     DRAVFLDAIAGFQEGFTAWAQIPAVVIAAVQGHAIGAGLQLALGADLRVVADDVAFAM
                     REPSLGMIPDMGGTHPLVRLVGYARALEICATGRFVHADEAVRIGLATVAVPAGALGE
                     TARELAGAMLAAHPGALRELKPLLQQAVDGDLASQLRAEREAQARLLGAAMAAPRE"
     gene            1064..>2368
                     /locus_tag="VHM65_03010"
     CDS             1064..>2368
                     /locus_tag="VHM65_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2704701.1"
                     /translation="MADDAVDDTRLLGGRPQAHPGDGPAGDGLRAPFRPQITAFLLVV
                     VVDSSLVVATPLLLKAIVDQGVIPRDSHVVVRLAVIVGLLAILDAILTVVQRFYSSRI
                     GEGLIYDLRTEVFAHVQRQPIAFFTRAQTGALVSRLNSDVIGAQQAFTSVLSSVVSNA
                     VSLVLIVGAMATLSWQLTLGALLLLPFFLVPARFMGRRLAGLAAQSMQLNADMGSRMT
                     ERFNVAGALLVKLYGRPEIEDREYAVRAAKVRDIGVRMSLNRTSFMVALGLVASLATA
                     MVYGFGGLMAVGGSLTVGTLLALTALPGRLYGPLTSISNVRVDVMTALVSFERVFEIL
                     DLRPKILQSPDARVLPSGPLSVEFDRVGFRYPSADEVSLVSLETTTSGDRKAGGTVLH
                     DITFTAPPGALVALVGPSGAGKTTITSLVSRLYDPTSGAVRLG"
BASE COUNT          287 a          860 c          828 g          393 t
ORIGIN      
        1 ggctcaccgc gagtcatcag cgggtcgtcg ccgtggcggt cgtcatcgcc gacggcgtgc
       61 tgcgtgaccg gatccgggtg gccgccgacg gcgccggcgt tgagctggtc ccaggtgtgc
      121 tcgctcacac cggggcgctg gcgaatctgg tgtcactgcg cgccggtaac ctcgaagggt
      181 gactgcgtcc gagccgacca tcccgaccca gaaccctgcg gatcagagca gcccccatcc
      241 ggccttgcgg gtgactcgga cccccgacct cgtcaccgtg accttgtgcg acccccggac
      301 ccgcaacgcc cagaccccga gcctctggct cgccctggcc gaggtcgccc gcagcatcgg
      361 ccccggggtg cgcgtcgtca tcatcgacgc cgaaggggag tccttctcgg ccgggctcca
      421 ccgcgggatg ctcagccccg ggggcctgcc tggggagccg ccggtgctgg ccctcgccgc
      481 gggggaccgg gccgtcttcc tcgacgcgat tgccgggttc caggagggct tcaccgcctg
      541 ggcgcagatc ccggccgtcg tcatcgccgc ggtccagggc cacgcgatcg gggccggctt
      601 gcagctcgcg ctcggtgccg acctgcgggt ggtcgccgat gacgtcgcgt tcgcgatgcg
      661 cgaacccagc ctcgggatga tcccggacat gggcggcacg catccgctgg tgcgcctcgt
      721 cggctatgcc cgggcgctgg agatctgcgc gaccgggcgc ttcgtccatg ccgacgaggc
      781 cgtgcggatc gggctggcca cggtcgcggt cccggccggt gccctcggtg agacggcacg
      841 cgagctggcg ggcgccatgc tcgccgctca tccgggcgct ctgcgcgagc tgaaaccgtt
      901 gctgcagcag gcggttgacg gtgacctcgc gagccagctg cgtgccgagc gggaggctca
      961 ggcgcgcctg ctcggagcgg cgatggcggc cccgagggaa tgaggcccgc gtcggtcacg
     1021 ttggacgcgc cggagacgag gaggcgctga tgagtatgtc gacgtggcag acgatgcggt
     1081 cgatgacacg cgactcctcg gtggccgacc gcaagctcac cccggggacg gcccggcggg
     1141 tgatgggtta cgcgcgccct tccgccctca gatcacggcc ttcctgctcg tcgtcgtcgt
     1201 cgactcgagc ctggtcgtgg cgaccccgtt actgctcaag gcgatcgtcg accagggcgt
     1261 catcccgcgg gactcacacg tcgtcgtgcg actcgccgtc atcgtcggcc tgctggcgat
     1321 cctcgacgcg atcctcaccg tcgtgcagcg cttctactcc tcgcgcatcg gggaggggct
     1381 gatctacgat ctgcggaccg aggtcttcgc gcacgtccag cgccagccga tcgcgttctt
     1441 cacccgggcg cagacgggtg ccctcgtctc acggctcaac agcgacgtca tcggggccca
     1501 gcaggcgttc acgtcggtgc tcagcagcgt cgtctccaac gcggtctcgc tcgtgctcat
     1561 cgtcggtgcc atggccaccc tgtcgtggca gctcaccctg ggggcgctgc tgctcctgcc
     1621 cttcttcctc gtccccgcga ggttcatggg caggcggctg gccgggctgg cggcccagtc
     1681 gatgcagctc aacgccgaca tgggcagccg gatgaccgag cgcttcaacg tggccggggc
     1741 gctcctcgtc aagctctacg gccggccgga gatcgaggac cgcgagtatg ccgtccgggc
     1801 cgccaaggtg cgcgacatcg gcgtgcgcat gtcgctcaac cggacctcgt tcatggtggc
     1861 cctcggcctg gtcgcctccc tggccacggc gatggtctat ggtttcggcg ggctcatggc
     1921 cgtcggcggc tcgctcacgg tggggaccct gctggcgctg accgcgctgc ccgggcgtct
     1981 ctacggcccg ctcacctcga tctcgaacgt ccgggtcgac gtcatgaccg cgctggtgtc
     2041 cttcgagcgg gtcttcgaga tcctcgacct gcggcccaag atccttcagt cccccgacgc
     2101 gcgggtgctg ccgagcggtc cgctctcggt ggagttcgac cgggtgggct tccgctaccc
     2161 ctcggccgac gaggtctcgc tcgtctcgct ggagacgacg accagtggcg atcgcaaggc
     2221 cggcggcacg gttctgcacg acatcacctt caccgcccct ccgggcgcgc tggtggccct
     2281 cgtcgggccg agcggagcgg ggaagacgac gatcacctcg ctcgtgtccc gcctctacga
     2341 cccgacgtcc ggtgccgtgc gtctcggc
//
LOCUS       DATAIX010000201         4988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_183789, whole genome shotgun sequence.
ACCESSION   DATAIX010000201 DATAIX010000000
VERSION     DATAIX010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183789"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..1103
                     /locus_tag="VHM65_03015"
     CDS             306..1103
                     /locus_tag="VHM65_03015"
                     /inference="COORDINATES: protein motif:HMM:NF019542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HEX2704702.1"
                     /translation="MPRSKPPPKKRPRPQPPAADGNALAIFGELTGLGSAPTQAQIAQ
                     FLAQGGFLGMALERLRPKPLTVPAPPKQLASYRVRVDLKGAKPPVWRRLDLAGDLRLD
                     QVNTVLQEAIGWYDLHLHSFRRWTDDVSLHFLTAYDIEEEGEGEGEEGLAEADVRLDQ
                     VLREVGDRLAYAYEFGDGWQLAIRLEAIRGRAADEPRARCVGGRRAGPPEDFGGIDWF
                     NEMVADWGDPRGRSRPGWLAQEDVPDWFDPKDFDPDELDERLRDLFQ"
     gene            complement(1269..1730)
                     /locus_tag="VHM65_03020"
     CDS             complement(1269..1730)
                     /locus_tag="VHM65_03020"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX2704703.1"
                     /translation="MTCIVARVFLDTNILAYQFDGSEPDKQARVRQLLSGHEHSFSVS
                     TQVLLELYQVITRKLTPTVPPSAARQVLTALAELAVVSADAQLVLRAAQTAEDDRLSI
                     WDAMIIEAAAEAGCDEVWTEDLHTGVRLRGVEIVNPLRTTTQDPLKTRLRP"
     gene            complement(1711..1944)
                     /locus_tag="VHM65_03025"
     CDS             complement(1711..1944)
                     /locus_tag="VHM65_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704704.1"
                     /translation="MANVTISVDEDTLRRARIRALERRESVNGYLAEMLRRYADGGSQ
                     ARVFRDLAAIADEAGAGRDGHGRAWTRDDLHRG"
     gene            complement(2023..2829)
                     /locus_tag="VHM65_03030"
     CDS             complement(2023..2829)
                     /locus_tag="VHM65_03030"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEX2704705.1"
                     /translation="MATHREGMATHRKASARAPRGYFEWEAAGLRWLAGAPGGAPVVE
                     VHGVGPGYLDLARLTSTTPTRAAAQRFGEQLAHTHRAGPAGGTAGPRGAYGSGPDGWE
                     GDGFFGPLSDPLPLSLTPHATWAQFYALERLAPITRACRDAGVLSADDAALLDRVGAG
                     LTEYDTCEPPARIHGDLWSGNIMWTADGATLIDPAAHGGHREADLAMLALFGAPHLEV
                     SIAAYQGVWPLASGWRERVALHQLYPLAVHALLFGGGYAAQTVAAARRYA"
     gene            complement(2874..3569)
                     /locus_tag="VHM65_03035"
     CDS             complement(2874..3569)
                     /locus_tag="VHM65_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2704706.1"
                     /translation="MRDGPQERAPLSRERVLRGALSIADAGGIGSLTIRSLAQELGVK
                     PMSVYHYVASKDEIIDGIVDLVFAEIDVPAAGGDWRSEMHRRAASARQMLRRHPWAIP
                     LLQSRTAPGPATLRHLNAVIGCLRGAGFSVKMTAHAYALIDSFVYGFALSEASLPING
                     PESVAEVAESMQERSSIAAYPHLLEFSTQHIMKPGYDFGEEFEFGLALVLDALADRAT
                     ARRTAPKRARARS"
     gene            3689..4735
                     /locus_tag="VHM65_03040"
     CDS             3689..4735
                     /locus_tag="VHM65_03040"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX2704707.1"
                     /translation="MRARGQATAIGGDTNSPAEGTMQAVVQDRYGSADVLRLARIARP
                     EVGDSEVLLRVHAAGLDRGTWHLMTGRPLLLRLVFGIRGPRQPVLGHDVAGTVVAVGS
                     AVTRFAAGDEVYGFGQGSFAEYAVAREDKLALKPANATFEQAAVVPVSALTAIQALCD
                     VGRVESGQKVLITGASGGVGSYAVQLAKAFGAEVTGVCSTAKLDLVRSLGATHVVDYT
                     RADFADGADRYDLILDIAGNPALSRLRRALTPTGTLVLVGGEEGGDWTGGMIERQLRT
                     RAVSLFVRQRLTSCLCTERGSDLERLTDLIEAGQVTPSIDRTYPLAKVPDAIRHLEAG
                     QARGKVAITTGLLR"
     gene            4732..>4988
                     /locus_tag="VHM65_03045"
     CDS             4732..>4988
                     /locus_tag="VHM65_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2704708.1"
                     /translation="MITVESLTKTYGAFTAVDDVAFTAEPGRVTGFLGPNGAGKSTTM
                     RILVGLTPATSGIAQVCGRRFADLPNPGLEVGVLLDASAQHA"
BASE COUNT          723 a         1736 c         1841 g          688 t
ORIGIN      
        1 gccttcgacc gcggcgcgct cggccggatc ggcctcgcta ccccgggtgg cgcggatcga
       61 ggtggatctg gccggtaagg gccggccgga tcggcctcgc taccccgggt ggcgcggatc
      121 gaggtggaac tggccggtcg ggagcagcca ttcgggactg cgccggaccg ctaggccgcg
      181 atccagggag tatcgtctgc tccccgggcg agccgcggtg gggagccacc gggtgcccgg
      241 caccgggcac ccggtggcga acgaaccagg tagggtccca ggaccgagga gaggaccagt
      301 ttcggatgcc acgatcgaag ccgccgccca agaagcgacc ccggccgcag ccacccgctg
      361 ccgacgggaa cgcgctggcc atcttcggtg agctgaccgg gctggggtcc gcgccgaccc
      421 aggcgcagat cgcgcagttc ctcgcccaag gtggcttcct cggcatggcc cttgagcggt
      481 tacgccccaa gccgctcacg gtgcccgcgc cacccaagca gctggccagc taccgggtcc
      541 gcgtcgacct caagggcgcc aagccgccgg tttggcgccg cctcgacctg gccggcgacc
      601 tgcgcctgga ccaggtcaac acggtcctgc aggaagccat cggctggtac gacctgcacc
      661 tgcactcctt ccgccggtgg accgacgacg tcagcctgca ctttctcacg gcatacgaca
      721 tcgaggagga gggtgagggt gagggtgagg aggggctggc cgaggcggac gtgcggctgg
      781 accaggtgct gcgggaggtc ggggaccgcc tggcctacgc gtacgagttc ggggacggct
      841 ggcagctggc gatccggctc gaggcgatcc gcgggcgagc cgccgacgag ccccgggcgc
      901 gctgcgtggg cgggcggcgg gccgggccgc cggaggactt cggcgggatc gactggttca
      961 acgagatggt cgccgactgg ggcgacccgc gcggtcgcag caggccgggg tggctggcac
     1021 aggaggacgt cccggactgg ttcgacccca aggacttcga cccggacgaa ctcgacgaac
     1081 gactgaggga cctgttccag tagccgcaag ccgggctcgc tgcagccagc ctggcgctgg
     1141 gcaggcgggc caaggccaat ggcatgcctg ctgcggtatg ccgcccgccg gcaccgctgc
     1201 tgagccgcgg ctcgctccac tcgccgcggt gcccttcagg cggggggcgg cggccgcgaa
     1261 ggagaacctc agggccgcag ccgtgtcttc agggggtctt gtgtcgtcgt gcgcaaaggg
     1321 ttgacgatct cgaccccgcg caaccggacg ccggtgtgga gatcctcggt ccagacctcg
     1381 tcgcacccgg cctcggcggc tgcctcgatg atcattgcgt cccagatcga cagccgatcg
     1441 tcctcggccg tctgcgcagc ccgcaagacg agctgtgcgt cagcgctgac cactgccaac
     1501 tcggcgagcg cagtgagcac ttgtcgggca gcggatggcg gcacggtagg agtcagcttg
     1561 cgcgtgatca cctgatagag ctcgagcagg acctgggtgc tgacgctgaa cgagtgctca
     1621 tgacccgaca gcagctgacg aacccgggct tgcttgtccg gctccgaccc gtcgaactga
     1681 taggcgagga tgttcgtgtc gaggaagacc ctagccacga tgcaggtcat cccgcgtcca
     1741 ggcgcggccg tgaccgtccc ggccggcacc ggcctcgtcg gcgatggccg cgaggtccct
     1801 gaagacccgg gcttggctcc ccccgtccgc gtagcgacgg agcatctcgg cgaggtagcc
     1861 gttgacggac tcgcgtcgct ccagcgccct gatccgagcg cgccgcagcg tgtcctcgtc
     1921 cacggagatc gtcacattcg ccatacgagc agtgtagcgc tgaatcagtg gagcacgacg
     1981 ccgatggccg atctcgtggc ccgctcgcat cgtgccaagg gttcacgcgt agcgacgcgc
     2041 ggcggcgacg gtctgggcgg cataccctcc gccgaagagc agcgcgtgga cggccagcgg
     2101 gtagagctgg tgaagggcaa cccgctcgcg ccagccgctg gccagtggcc aaaccccttg
     2161 gtatgccgcg atgctcacct ccaggtgggg cgcaccgaag agagcgagca tggccaggtc
     2221 ggcctcgcga tgaccgccgt gagcggccgg gtcgatgagg gtggcgccat cggctgtcca
     2281 catgatgttg cccgaccaca ggtcgccgtg gatgcgggcg ggcggctcgc aggtgtcgta
     2341 ctcggtgagg ccggccccga ctcggtcgag cagagcggcg tcatccgccg agaggacgcc
     2401 cgcgtcgcgg caggcgcgcg tgatgggagc gaggcgctcc agcgcataga actgcgccca
     2461 ggtcgcgtgc ggggtgagcg agagggggag cgggtccgaa agcgggccga agaagccgtc
     2521 accctcccag ccgtccggcc cgctcccgta tgcgccccgc gggcccgcgg tgccccctgc
     2581 cgggccggcc cggtgggtgt gggccagctg ctccccgaac cgttgggcgg ccgctcgggt
     2641 gggcgtggtc gaggtgagcc gggcgaggtc gaggtagccg gggccgacgc cgtggacctc
     2701 gacgaccggt gcaccgcccg gggcgccggc cagccagcgc agccccgcgg cctcccactc
     2761 gaagtagccg cgcggggcgc gcgcgctggc cttgcggtgc gtcgccatac cctcgcggtg
     2821 cgtcgccata cctgctgagc gtagacggcg tgagggggcg acgcggcggc ggctcagctc
     2881 ctcgcgcggg cgcgcttcgg ggcagtccgg cgggccgttg cccgatccgc gagggcgtcg
     2941 aggaccaggg cgagcccgaa ctcgaactcc tcgccgaagt cgtagccggg cttcatgatg
     3001 tgctgggtcg agaactcgag caggtggggg tacgcggcga tggaggaccg ctcctgcatc
     3061 gactcggcga cctcggcgac ggactccggg ccgttgatgg gcagggaggc ctccgagagc
     3121 gcgaacccgt agacgaagct gtcgatcagg gcgtaggcgt gggcggtcat cttcaccgag
     3181 aagcccgccc cgcgcaggca gccgatgacc gcgttgaggt ggcgcagcgt cgccgggccg
     3241 ggcgcggtcc gcgactgcag gagcgggatc gcccaggggt ggcgcctcag catctgacgg
     3301 gccgaggcgg cccgccggtg catctcagat cgccagtcgc cgccggcggc cgggacgtcg
     3361 atctcggcga agacgagatc gacgatgccg tcgatgatct cgtccttgct ggcgacgtag
     3421 tggtagacgg acatcggctt gacgcccagc tcctgcgcga gcgagcggat cgtcagcgat
     3481 ccgatgcccc ccgcgtcggc gatggacagg gcaccgcgca gcacccgctc gcggctcagc
     3541 ggtgcccgct cctgagggcc gtccctcact ggtttcacca tgcgcttctc accattgctt
     3601 ccagacccta gcgggttcgt acttagtacg ataccgtact cgtactaagt acggcgggag
     3661 cggctcgccg actggaaggg aagcggtcat gagagctcga ggacaggcca ccgccatcgg
     3721 cggcgacacg aacagcccgg cggagggcac gatgcaggcg gtcgtgcagg acaggtacgg
     3781 ctcggccgac gtcctgcgcc tcgcgcggat cgcccggccc gaggtgggcg acagcgaggt
     3841 gctccttcgg gtgcacgcgg ccggtctcga ccggggcacg tggcacctca tgacggggcg
     3901 gcccctcctg ctccgcctcg tgttcgggat ccgcgggccg aggcagccgg tcctcgggca
     3961 cgacgtggcc ggcaccgtcg tcgcggtcgg ctcggcggtg acgaggttcg ccgccggtga
     4021 cgaggtgtac ggcttcggtc agggctcctt cgccgagtac gccgtggccc gcgaggacaa
     4081 gctggcgctc aagccggcca acgcgacctt cgagcaggcc gcggtggtcc cggtctcggc
     4141 gctgaccgcc atacaggccc tgtgcgacgt cggccgggtc gagtcgggac agaaggtact
     4201 gatcaccggt gcgtccgggg gcgtcgggtc gtatgccgtc cagctcgcca aggcgttcgg
     4261 ggccgaggtc accggcgtct gcagcaccgc caagctcgac ctggtccggt ccctcggcgc
     4321 cacccacgtc gtcgactaca cccgggcgga cttcgccgat ggtgcggacc gctacgacct
     4381 gatcctcgac atcgccggca accccgccct ctcccggctg cggcgcgcgc tcacgccgac
     4441 gggcacgctc gtcctcgtcg gcggcgagga gggcggcgac tggaccggag gcatgatcga
     4501 gcggcagctg cggacccggg ccgtgtcgct cttcgtgcgg cagcgcctga ccagctgcct
     4561 ctgcacggag cgtggcagcg acctcgagcg gctcaccgac ctcatcgagg ccggccaggt
     4621 gacgccgagc atcgaccgga cctacccgct cgccaaggtg ccggacgcca tacgccacct
     4681 cgaggccggc caggcccgcg ggaaggtcgc catcacgacg gggctcctcc gatgatcacg
     4741 gtcgagtcac tcaccaagac gtatggcgcc ttcaccgccg tcgacgacgt cgccttcacc
     4801 gccgagccgg ggcgggtcac cggcttcctc ggcccgaacg gcgcgggcaa gtcgacgacg
     4861 atgcggatcc tggtgggcct cacgcccgcc acctcgggca tcgcgcaggt ctgcgggcgc
     4921 cgcttcgcgg acctgcccaa ccccggcctc gaggtcggcg tcctgctcga tgcctccgcc
     4981 cagcacgc
//
LOCUS       DATAIX010000202         4964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_100733, whole genome shotgun sequence.
ACCESSION   DATAIX010000202 DATAIX010000000
VERSION     DATAIX010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100733"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VHM65_03050"
     CDS             complement(<1..763)
                     /locus_tag="VHM65_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704709.1"
                     /translation="MTSLTHALSTLASHPRAVAAATVAREACTELRWHEALRRRIPEA
                     CAESRIRGARASAMLDGARLPLERVREMVLGLGQGGDLDPVERVVMGAVAATSESERL
                     RAVVRRAPYQGLARLHVAATAGLLPADQVGRPRAEGEACAEVVDLGAAPGVDVVAERL
                     AALADIVAAVSQGLPALAVAAIVHAEVMTVRPFVAGNGLVARAMERAIVAAFGLDPTG
                     VAVPETGHERAGLAGYLGALTAYGSGGLDGVLIWLE"
     gene            786..1970
                     /locus_tag="VHM65_03055"
     CDS             786..1970
                     /locus_tag="VHM65_03055"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX2704710.1"
                     /translation="MSITISLPVALLMAAAVVVGLRLLTRPASRRAFVSHGEQATYET
                     LHTASLAARHLGEGLTPQGTAKAGKHLRSLLGVEALAFVDSTGVLSWDGAGANHRHDA
                     PGHAEPVLRQGRTVVLTDLGCPDPSCPVRSAVLAPVVADDRVVATLVAYDKSASTGLV
                     RATEEVARWSATQAEVAELGRERTRTMEAGLRALRAQISPHFIYNCLAAIASFVRTDP
                     DRARELLLEFADFTRYAFRRGGAYTTLADELRNIERYLVLEQARFGDRLTVSLTIAPE
                     VLAVAVPFLSIQPLVENAVRHGLATKSGSGHVTITAGDAGPVAEISIEDDGIGSDPDV
                     IRAVLSGESTVDSVGLGNVDARLRQAFGDQHGLVVETAPGAGTKVSFRVPKFAPGVTT
                     GS"
     gene            2010..2783
                     /locus_tag="VHM65_03060"
     CDS             2010..2783
                     /locus_tag="VHM65_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX2704711.1"
                     /translation="MTDPPAGGSAGPRLRALLVDDEAPTLSELAFLLRQDERIETIHT
                     ATSGTEALKMIESHPVDVVFCDILMPGLDGMDLARVLARFTERPQVVFVTAYDAHAVD
                     AFDLDATDYVMKPARPERLAEAVRRVLDKRADRARGQGEAPAAPDETIPVELGGRTTF
                     IQRSQIRYAQAQGDYARLHTETDSHLVRISLNALEERWSEHGFVRIHRSTLVATPHVT
                     AVSMLHGHCSVSLGDVELQVSRRHTRELRDRLVRLPQLP"
     gene            2780..3247
                     /locus_tag="VHM65_03065"
     CDS             2780..3247
                     /locus_tag="VHM65_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704712.1"
                     /translation="MSSPGPGAPRPRRLGLPSSSGPGGSTGPKPGIAPPHRVRVTSPR
                     RDARRGSDPRPRSTELKEQTGLGEIYLSALLRAQLRLSATVLAGAAVVLGGLPVLFLA
                     FPEIRRVHLGPVPLPWVILGVLVYPAIALIARAYVRQAERIEREFSDLIRERQ"
     gene            3244..4887
                     /locus_tag="VHM65_03070"
     CDS             3244..4887
                     /locus_tag="VHM65_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEX2704713.1"
                     /translation="MTGRDPSYAIAAVTVVSLATLLIGGIGWRLSRTTRDFYVAGRSV
                     SPGWNASAIGGEYLSAASFLGVAGLVYAYGVDMLWFPVGYTAGYLVLLVLVAAPLRRS
                     GAYTIPDFAEVRLESRLVRRIAAALVVGIGWLYLLPQFQGAGLALSTVTGAPRWVGAV
                     IVAVVVVTNVVAGGMRSITFVQAVQYWIKLTAISIPAFVCLWLWTRDGQPAPDLPAVL
                     RTTWVEPMTGARAHSVYATYSTILALCFGTMGLPHVLVRFYTNPDGRGARRTTLAVIG
                     LLGLFYLFTPVYGVLGRVYLPSLPEGVRADAVTLLLPDAISPGTLGSLLSAVLAAGAF
                     AAFLSTASGLTISVAGVIDQDLLGPRLSRIAGGDTRRIQHFRLATLVAVLIPYLLSWL
                     TLSSSLADTVGLAFAVAASTFCPLLVLGVWWPRLSVAGAAAGMASGGALAIAATLVTV
                     LGLGDGGTAWHGWLTALLAQPAAWTVPIAFLVASSSGWPRRGRCPAGPRRRWSACTPP
                     SPSRHPERPSRLPAPRPGARPGLPSRRHLPPEVRRPRRR"
BASE COUNT          622 a         1929 c         1671 g          742 t
ORIGIN      
        1 gttcgagcca gataagcacc ccgtccagac ctcccgaccc gtaggccgtc agtgcgccga
       61 ggtaccctgc gagcccagcc cgctcgtgtc cggtctcggg cacagccacc ccggtcgggt
      121 ccagcccgaa cgccgccacg atcgcgcgtt ccatggcgcg cgcgacgagc ccgttgcccg
      181 caacgaacgg ccggacggtc atgacttcgg catgcacgat ggccgccacc gccagagcgg
      241 gcagcccctg cgacacggcc gcgacaatgt cagccagggc cgccagccgc tcggccacca
      301 catccacgcc gggagccgcc ccgaggtcga ccacctcagc gcacgcctcg ccctcggcgc
      361 gcggccgccc cacctggtcg gccgggagca gtcctgcggt tgccgcgacg tgcaaccggg
      421 ccaggccctg gtatggcgcc ctcctcacca ccgcgcgcag acgctcactc tcggacgtcg
      481 ccgcgacggc gcccatgacg acgcgctcca cggggtcgag gtcgccgccc tgaccgaggc
      541 ccagaaccat ctcccgcacc cgctcgagcg ggagccgagc cccgtcgagc atcgcgctcg
      601 cccgagcccc gcgaatccgg gactcggcgc acgcttccgg gatccggcgg cgcagggcct
      661 catgccagcg cagctcggtg caggcctctc gcgccacggt ggcggccgcg accgcccgcg
      721 ggtgactggc gagggtcgag agcgcatggg tgagcgatgt caccgctcca ccttagagtt
      781 cccccatgag catcaccatc tcgctcccgg tggcactgct catggccgct gcggttgtcg
      841 tcgggctcag gctgctcacc cggccggcct cccgccgcgc cttcgtctcc cacggtgagc
      901 aggcgacata cgagacgttg cacaccgcct cactggcggc caggcacctc ggcgaggggc
      961 tcactcccca aggcacggcc aaggcaggca aacatctgcg ctcgctgctc ggcgtcgagg
     1021 cgctggcgtt cgtggactcc acgggggtgc tgtcctggga cggcgccggg gcgaaccatc
     1081 gccacgacgc cccggggcac gccgagccag tgctgcgcca gggccgcacg gtcgtgctca
     1141 ccgacctcgg ctgccccgac cccagctgcc cggtgcgctc ggcggtcctt gcgccggtcg
     1201 tcgccgacga ccgcgtcgtg gcgaccctcg tggcgtacga caagagcgcg tcgaccgggc
     1261 tcgtgcgcgc cacggaggag gtcgcccgct ggtccgcgac ccaggccgag gtggccgagc
     1321 tgggtcgcga gcgcacgcgc acgatggagg ccgggctgcg agcgttgcga gcgcagatca
     1381 gcccccactt catctacaac tgcctggcgg cgatcgcctc gttcgtgcgc accgaccccg
     1441 accgggcccg cgagctgctg ctggagttcg ccgacttcac ccgttacgcc tttcgtcgcg
     1501 gaggtgccta caccaccctg gccgacgagc tgcgcaacat cgagcgctac ctcgtcctcg
     1561 agcaggcccg cttcggcgac aggctcaccg tgagtctcac cattgcgccc gaggtcctcg
     1621 ccgtggccgt cccgttcctg tcgatccagc cgctggtcga gaacgccgtg cgccacgggc
     1681 tggccaccaa gagcgggtct ggccatgtga cgatcaccgc cggtgatgcc ggcccggtcg
     1741 ccgagatcag catcgaggac gacgggatcg gcagcgaccc cgacgtgatc cgggcggtgc
     1801 tctccgggga gagcaccgtc gacagcgtgg ggctcggcaa cgtcgacgcg cggctgcgcc
     1861 aggccttcgg cgaccagcat ggcctcgtcg tcgagacggc cccgggcgcg ggcaccaaag
     1921 tgagcttccg ggtcccgaag ttcgcccctg gagtcacaac cggcagctga gggcccccgc
     1981 ggaggggctg ggcgccgtag tctgccgcca tgaccgaccc accagccggg ggctcagccg
     2041 gccctcggct gcgcgccctg ctcgtcgacg acgaggcacc caccctgtcc gagctggcct
     2101 tcctgcttcg ccaggacgag cggatcgaga cgatccacac cgcgacgagc ggcaccgagg
     2161 cgctcaagat gatcgagagc cacccggtgg acgtcgtctt ctgtgacatc ctgatgcccg
     2221 gcctcgacgg gatggacctt gcccgtgtcc tcgcccggtt caccgagcgc ccgcaggtcg
     2281 tcttcgtcac cgcgtacgac gcgcacgcgg tcgacgcttt cgacctcgac gccaccgact
     2341 acgtcatgaa gccggcccgc cccgaacgcc tcgccgaggc ggtccgccgg gtcctcgaca
     2401 agcgtgccga ccgcgcccgc ggccagggcg aggccccggc ggcaccagac gagacgatcc
     2461 cggtcgagct cggcgggcgc accacgttca tccagcgcag ccagatccgc tacgcccagg
     2521 ctcagggcga ctacgcccgc ctgcacaccg agaccgactc gcacctcgtc cggatctccc
     2581 tcaacgccct cgaggagcgc tggagcgagc acggcttcgt ccgcatccac cgcagcacgc
     2641 tcgtggccac ccctcatgtc acggccgtct cgatgctcca cggtcactgt tcggtcagcc
     2701 tcggtgacgt cgagctgcag gtgagccggc gccatacccg cgagctgcgc gaccggctcg
     2761 tccgcctgcc ccagctccca tgagcagccc cggaccgggc gcaccgcggc cccggcggct
     2821 gggtctgccg tcctcgtccg gtccaggcgg gtccaccggc ccgaagccgg gtatagcgcc
     2881 accgcatcgc gtccgggtca cctcgccgcg gcgcgatgcg cggcggggca gcgacccccg
     2941 gccccggagc accgagctga aggaacagac cgggctgggt gagatctacc tgtcggcgct
     3001 gctcagggcc cagctgcggc tgtcggcgac cgtcctggcc ggagccgcag tcgtcctcgg
     3061 gggtctgccg gtgctcttcc tcgcctttcc cgagatccgc cgggtccacc tcggcccggt
     3121 cccgttgccg tgggtgatcc tcggggtcct cgtctacccg gccatcgccc tcatcgcgcg
     3181 cgcgtacgtc cgccaggccg aacggatcga gcgcgagttc agcgacctga tccgggagcg
     3241 gcagtgacgg ggcgcgaccc gtcatacgcg atcgcagccg tcacggtcgt cagcctcgcc
     3301 acgttgctca tcggcgggat cggatggcgc ctgtcccgca cgacccgcga cttctacgtc
     3361 gcggggcgct cggtctcgcc gggctggaac gcctcggcca tcggcggcga gtacctctcg
     3421 gcggcgtcgt tcctcggcgt cgccgggctc gtctacgcct acggcgtcga catgctgtgg
     3481 ttccccgtcg gctataccgc gggatacctc gtcctgctcg ttctcgtcgc cgcccccctg
     3541 cgccgctccg gcgcctacac gatcccggac ttcgccgagg tccggctcga gtcgcgcctc
     3601 gtccgtcgca tcgccgcggc gctcgtcgtc gggatcgggt ggctctacct gctcccccag
     3661 ttccagggag ccgggctggc cctgagcacc gtgaccgggg cacctcgctg ggttggcgcg
     3721 gtgatcgtcg cggtcgtcgt cgtcaccaac gtcgtcgcgg gagggatgcg ctcgatcacc
     3781 ttcgtccagg ccgtacagta ctggatcaag ctcaccgcca tctcgatccc cgccttcgtc
     3841 tgtctctggc tctggacccg cgacgggcag cccgcccccg acctgcctgc cgttctgcgc
     3901 acgacctggg tcgagccgat gaccggcgcc cgcgcgcact cggtctacgc gacgtactcg
     3961 acgatcctgg cgctctgttt cgggacgatg ggcctgccgc atgtgctcgt ccgcttctac
     4021 accaacccag acggacgggg ggcgcggcgc acgaccctcg cggtcatcgg gttgctgggc
     4081 ctgttctacc tcttcacccc ggtgtatggc gtgctcgggc gggtctatct gccgagcctg
     4141 cccgagggcg tgcgcgccga cgcggtgacg ttgctcctgc ccgacgcgat atcacccggg
     4201 acgttgggct cgctgctgtc ggccgtgctc gcggcagggg cgttcgcggc gttcctgtcc
     4261 accgcgtcgg gtctgacgat ctcggtagcc ggcgtcatcg accaggacct gctcggccct
     4321 cggctgagcc ggatcgcggg cggggacacc cgccggatcc agcacttccg gctcgctacg
     4381 ctggtcgcgg tcctcatccc ctacctgctg tcctggctca ccctcagctc ctcgctggcc
     4441 gacacggtgg gcctcgcgtt cgccgtggcg gcctcgacct tctgcccgct gctcgtgctg
     4501 ggcgtctggt ggccgaggct ctcggtcgcc ggggcggcgg ctggaatggc cagtgggggt
     4561 gccttggcga tcgccgcgac cctggtgacc gtgctcgggc tcggtgacgg gggcaccgcg
     4621 tggcacggct ggctgaccgc gttgctcgcc cagccggccg cctggacggt gccgatcgcc
     4681 ttcctcgtgg catcgtcgtc tggctggcca cgccggggtc ggtgccccgc gggaccgcgc
     4741 agacgatggt ccgcctgcac gcccccgagt ccgtcgcgtc accctgaacg gcctagccgg
     4801 ctgccggcgc cccggccggg ggcccgccct ggcctcccgt cgaggcgcca cctcccgccg
     4861 gaggtccgcc ggccccgccg ccggtgatgc gacggaggtt ctgcgtcgag ttggtcggca
     4921 gctccgcggt gcggtcggcg aggacgacaa cggcaccctc agac
//
LOCUS       DATAIX010000203         1656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_100765, whole genome shotgun sequence.
ACCESSION   DATAIX010000203 DATAIX010000000
VERSION     DATAIX010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100765"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VHM65_03075"
     CDS             complement(<1..610)
                     /locus_tag="VHM65_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX2704714.1"
                     /translation="MSCAGVEDVASGADRVFVERASEHIHVAASLMKLPLVVAAYRLA
                     DRGLLDLDAPVPVHNVHASALDGAPYACTPDYDQDDATWDRQGSAAPLRWLCRQAIVR
                     SGNLATNLVLEHVRRAGWGEVARALSECDAAHGTVVSRGIQDEAAAAAGLANLVTAGD
                     MGRLMRAIARGAAASPASCAELVEVLDAQEWRDGIAAGVPAGT"
     gene            complement(687..989)
                     /locus_tag="VHM65_03080"
     CDS             complement(687..989)
                     /locus_tag="VHM65_03080"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2704715.1"
                     /translation="MILLRREFGDVLRENRRAQGRTLREVSASASVSLGYLSEVERGE
                     KEASSELLASICAALRLPLSEVLSDVSARVAESEALVAPVTLPVAGVAPLVSASAA"
     gene            complement(1196..>1656)
                     /locus_tag="VHM65_03085"
     CDS             complement(1196..>1656)
                     /locus_tag="VHM65_03085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HEX2704716.1"
                     /translation="LTEAGQSLACAESLTAGLLCARVADVAGASAVLRGGLVTYATDL
                     KASILGVDPDLLARRGAVDPEVAATMAGSVRVLLGATWGVATTGVAGPTEQDGHPVGE
                     VYVAVAGPRGEGGVVGLGVRALRLAGSRAQIREQAVAAALRLLLERLDEG"
BASE COUNT          245 a          631 c          592 g          188 t
ORIGIN      
        1 gggtgccggc tgggactccc gccgcgatcc cgtcgcgcca ctcctgggcg tcgagcacct
       61 cgacgagctc ggcacaggag gcagggctcg cggcggcgcc ccgcgcaatg gcccgcatga
      121 gccggcccat gtccccagcc gtgacgaggt tggccaggcc ggccgccgca gcggcctcgt
      181 cctggatgcc ccgggacacc acggtgccgt gggcggcgtc acactcgctc agggctcgcg
      241 ccacctcgcc ccaccccgcg cggcgcacgt gctcgaggac gaggttcgtc gcgaggttgc
      301 ccgagcggac gatcgcctgg cggcagagcc acctcagcgg ggccgcgctg ccctggcggt
      361 cccacgtcgc gtcgtcctgg tcgtagtccg gggtgcaggc gtacggcgca ccgtccagcg
      421 cagaggcgtg gacgttatgg accggcacag gtgcgtcgag gtcgagcagg ccgcggtcgg
      481 cgaggcggta ggcagcgacg accagaggga gcttcatgag gcttgcggcg acgtggatgt
      541 gctcgctggc gcgctcgacg aacacccggt ccgctccgct cgcgacgtcc tccaccccgg
      601 cgcaagacac cgacagcgtg ccgggcccgg acgtcagccg cccaagagca gcgagaacag
      661 ggcccgagtg gggcaccgac gtgtggtcag gccgccgagg ccgagacgag cggggcgacg
      721 ccagcgaccg ggagcgtgac gggcgcgacg agggcttcgc tctcggcgac ccgagcggag
      781 acgtcactga ggacctcgga caggggcagg cgcagcgcgg cgcaaatcga ggcaagcagc
      841 tcggaggagg cttccttctc gccgcgctcg acctcgctga gatagccgag ggacaccgac
      901 gccgaggcgg acacctcacg cagggtgcgg ccctgggccc ggcggttctc gcgcagcaca
      961 tcgccgaact cgcgtcgcaa caggatcacg tcaccctcct ctgctcactg cctcggtcgt
     1021 cctcgcagga cacgagcgcc ctcgcggccc taccggaacc aacggtacca acggtacccg
     1081 ggtctccacg gtaccgcctg gcattggtga ccggtgtccc gggtgtcggg caagtcggcg
     1141 accccgacgt gccactgggt cacccaacgc aggcaaaccg attcaaacgg gcaggtcagc
     1201 cctcgtcgag ccgttccagc agcagccgca gggccgcggc cacggcctgc tcccggatct
     1261 gggcccgcga gcccgccagc cgcagcgctc gcacgcccag gcccacgact ccgccctcgc
     1321 cccgagggcc cgcgacggcc acgtagacct cccccacggg gtggccgtcc tgctcggtcg
     1381 gcccggccac gcccgtcgtc gcgacccccc aggtggctcc gagcagcacc cggacgctgc
     1441 ccgccatcgt cgcggccacc tcggggtcga cggcgccgcg acgcgcgagc aggtccgggt
     1501 cgacacccag gatcgacgcc ttgagatcgg tggcgtaggt gacgaggccc ccgcgcagga
     1561 cggccgaggc cccggcgacg tccgccaccc gcgcacagag cagccccgcc gtgagcgact
     1621 cggcgcaggc gaggctctgt ccggcctcgg tgagca
//
LOCUS       DATAIX010000204         2068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_100814, whole genome shotgun sequence.
ACCESSION   DATAIX010000204 DATAIX010000000
VERSION     DATAIX010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100814"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..>2068)
                     /locus_tag="VHM65_03090"
     CDS             complement(14..>2068)
                     /locus_tag="VHM65_03090"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEX2704717.1"
                     /translation="GKTDTIARLDATAAQGIAEALHEAVIRVTSVQEKPYTRRPSAPF
                     TTSTLQQEASRKLRMASKTAMRVAQRLYENGFITYMRTDSTTLSEAAITAARSQARDL
                     YGASYVPDAPRRYEGKVKNAQEAHEAIRPAGDRFRTPAQVAGELRGDEFALYELIWKR
                     TVASQMADATGSTATVRLGAALAGLAAAHEVELSASGTVITFRGFLAAYEEGRDEAQA
                     RRKRDIAEEERRLPKLSEGVPVHLVKAGAEGHETNPPPRYTEATLVKAMEEKGIGRPS
                     TYASTVGTIQDRGYVTTRGQALLPTWLAFAVTRLLEEHFATLVDYDFTASMEEDLDRI
                     ASGDEHRVAWLSRFYFGDEAASLDGLRHLVDDLGEIDAREISTIPIGEGIVVRVGRYG
                     PYVEEVTPAGVDPVTGEAADGATSDPRRATITDDIAPDELTPEKARELLAAAADDGRV
                     LGQDPETGRDIVARAGRYGPYVTEVLDEEAAGATAKSKVRAKPRTASLFTGMDLATVD
                     LDTAMRLLSLPRVVGADAEGLEITAQNGRYGPYLKRGTDSRSLTTEQQLFEITLEEAL
                     AIYAQPKTRGRAAAAAPLKELGPDPVSGKPVVVKEGRFGPYVTDGETNATLRKGDEPE
                     TITAERGSELLAEKRARGPVTRKRTPATTTAAKKATTKRTTAAKKTAAAQTPAKRSAP
                     RG"
BASE COUNT          232 a          714 c          768 g          354 t
ORIGIN      
        1 acccaggccc ggctcagccc cgaggtgccg agcgtttcgc tggcgtctgt gcggccgccg
       61 tcttcttggc cgccgtcgtc cgtttggtgg tggccttctt cgcggccgtc gtcgtggcgg
      121 gggtgcgttt gcgggtcacc ggcccccggg cgcgcttctc ggccaggagc tcggaacctc
      181 tctcggcagt gatggtctcg ggctcgtcgc ccttgcgcag cgtcgcgttg gtctccccgt
      241 ctgtgacgta ggggccgaac cggccctcct tgacgacgac gggcttgccg ctgaccgggt
      301 cgggaccgag ctccttgagc ggcgcggcgg ccgcggcgcg gcccctggtc ttgggctggg
      361 catagatcgc gagcgcctcc tcgagggtga tctcgaagag ctgctgctcc gtggtcagcg
      421 accgggagtc ggtgccgcgc ttgagatagg gcccatagcg gccgttctgg gcggtgatct
      481 ctagcccctc ggcgtcggcg ccgaccacgc ggggcagcga cagcagccgc atggccgtgt
      541 cgaggtcgac cgtcgcgagg tccatgcccg tgaagagcga ggccgtccgc ggcttggccc
      601 tgaccttgct cttggccgtt gcgccggctg cctcctcgtc gagcacctcg gtgacgtaag
      661 ggccgtagcg gccggcccgg gcgacgatgt cgcgcccggt ctctgggtcc tggccgagca
      721 cccggccgtc gtcggcggcc gccgccagca gctcccgggc cttctcgggg gtcagctcgt
      781 cgggtgcgat gtcgtcggtg atcgtcgcac ggcgtgggtc gctcgtcgcg ccgtcggccg
      841 cctcaccggt gaccgggtcg acgcctgcgg gggtgacctc ctcgacatac gggccgtagc
      901 gtccgacccg gacgacgatc ccctcgccga tcgggatcgt cgagatctcc cgggcgtcga
      961 tctcgcccag gtcgtcgacg agatggcgca gaccgtcgag ggaggcggcc tcgtcgccga
     1021 agtagaagcg gctcagccac gcgacgcggt gctcgtcgcc gctggcgatg cggtcgaggt
     1081 cctcctccat cgaggcggtg aagtcgtagt cgacgagggt ggcaaagtgc tcctccagca
     1141 gccgggtcac cgcgaaggcg agccaggtcg gcaggagcgc ctgcccacgg gtggtgacat
     1201 agccgcggtc ctggatggtc ccgaccgtcg aggcgtatgt cgaggggcgg ccgatcccct
     1261 tctcctccat ggccttgacg agagtcgcct cggtatagcg cggaggcggg ttcgtctcgt
     1321 ggccctcggc gccggccttg accagatgga cgggcacgcc ctcagagagc ttgggcaggc
     1381 ggcgctcctc ctcggcgatg tcccgcttgc gcctggcctg ggcctcgtcg cggccctcct
     1441 cataggccgc gaggaacccg cggaaggtga tgacggtgcc gctcgccgac agctcgacct
     1501 cgtgagcggc cgcgaggccg gccagggccg cgccgagcct caccgtcgcc gtcgagcccg
     1561 tggcatcggc catctgcgag gccacggtgc gcttccagat cagctcgtag agggcgaact
     1621 cgtccccacg cagctccccc gccacctgcg cgggggtgcg gaagcggtct cctgccgggc
     1681 ggatcgcctc gtgcgcctcc tgggcgttct tgaccttgcc ctcgtagcgg cgcggggcgt
     1741 cggggacgta cgacgcgcca tacaggtccc tcgcctggct tcgcgcggcg gtgatcgcgg
     1801 cctcggacaa ggtcgtcgag tcggtccgca tgtaggtgat gaagccgttc tcgtagagcc
     1861 gctgggccac ccgcatcgcg gtcttggagg ccatccgcag cttgcgcgag gcctcttgtt
     1921 gcagcgtcga ggtggtgaag ggggccgacg gccggcgggt gtagggcttc tcctggacgc
     1981 tcgtgacgcg gatcacggcc tcgtgcagcg cctcggcgat cccctgggcg gcagtggcgt
     2041 cgaggcgcgc gatggtgtcg gtcttgcc
//
LOCUS       DATAIX010000205         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_183882, whole genome shotgun sequence.
ACCESSION   DATAIX010000205 DATAIX010000000
VERSION     DATAIX010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183882"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VHM65_03095"
     CDS             <1..445
                     /locus_tag="VHM65_03095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX2704718.1"
                     /translation="PEVLKEGSGGLTELLTTPEQRARIAALTAVMALLEGHADVVMDD
                     VGPQVIPTVAEIRAKFTERRKGLSSLDRLVRRLLGLEAKMSQYRNGAAFVRGVVDRIG
                     IDDFNAIWTSPQTLPSPEEIATPAHWIDRVHGADRVPGADRVPGA"
     gene            442..1449
                     /gene="tilS"
                     /locus_tag="VHM65_03100"
     CDS             442..1449
                     /gene="tilS"
                     /locus_tag="VHM65_03100"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX2704719.1"
                     /translation="MTLTERPETLAAVDPSVPTAWAAVRSELADLDPGSLVVVACSGG
                     PDSLALAAAAAVEGRRTGVRVGAVVIDHGLQDGSTDVAHRVAEQCRSLGLEPVSVVTV
                     SVRQSGQGPEAAARQARYGGLRDAAAALGAVRVLLGHTRDDQAEQVILGLARGSGPRS
                     LSGMPRRRDCFARPFLGLTHAETEAVCAAYGLEPWTDPHNADPAYRRVRARALIRAFE
                     AELGPGIAKSLARTADLCREDGELLDAQAEAARAGLGAVPVAVADLAALPRPIRTRVW
                     RVLATEVGSPAGSLTACHVESLDTLVTTWHGQGPLHLPGHVQARRVGGRVRIEGPGPV
                     E"
     gene            1504..2055
                     /gene="hpt"
                     /locus_tag="VHM65_03105"
     CDS             1504..2055
                     /gene="hpt"
                     /locus_tag="VHM65_03105"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX2704720.1"
                     /translation="MDATDMGPDLEKVLLTEEQILARLDEMAAQICADYQGKDLLLVG
                     VLKGAVIVMADLMRALPVSVPMDWMAISSYGSGTKSSGVVRIIMDLRDDITDRHVLIV
                     EDIIDSGLTLNWIRNNLLSRNPASLEICTLLRKPDVAKVDIDVRYVGFDIPNEFVVGY
                     GLDYGERYRNLRQVATLAPHVYS"
BASE COUNT          294 a          820 c          760 g          301 t
ORIGIN      
        1 ccccgaggtg ctcaaggaag gcagcggcgg cctcaccgag ctcctcacga cccccgagca
       61 gcgcgcgcgg atcgccgcgc tcaccgcggt catggccctg ctcgaaggcc acgccgacgt
      121 cgttatggac gacgtgggcc cgcaggtcat cccgaccgtg gccgagatcc gggccaagtt
      181 caccgagcgc cgcaaggggc tcagctcgct cgaccggctg gtccggcggc tgctggggct
      241 cgaggcgaag atgagccagt accgcaacgg cgcggccttc gtccgcggcg tcgtcgaccg
      301 catcggcatc gacgacttca acgcgatctg gacctccccc cagaccctgc ccagtcccga
      361 ggagatcgcg accccggcgc actggatcga ccgggtgcac ggcgccgatc gggtgcccgg
      421 cgccgatcgg gtgcccggcg catgacgctc accgagcgcc ccgagacgct cgctgccgtg
      481 gaccccagcg tcccgaccgc ctgggcggcg gtgcgctccg agctcgccga tctcgacccc
      541 ggctcgctcg tcgtcgtcgc ctgcagcggc gggcccgact cgctcgcgct cgcggctgcg
      601 gccgcggtcg aggggcgccg gaccggtgtg cgggtcggtg cggtcgtcat cgaccacggt
      661 ctgcaggacg gctccacgga cgtggcccac cgcgtggccg agcagtgccg gtccctcggc
      721 ctggagccgg tgagcgtcgt cacggtcagc gtccgccagt cgggccaggg gcccgaggcg
      781 gcggcccgcc aggcccggta tggcgggctc cgcgacgccg cggcagccct cggcgcagtc
      841 cgggtcctgc tggggcacac ccgcgacgac caggccgaac aggtcatcct cggtctcgcg
      901 cggggctccg gcccgcgctc gctgtcgggc atgccccggc gccgggactg cttcgcgcgg
      961 ccgttcctcg gcctgaccca cgccgagacc gaggccgtgt gcgcggcata tgggctggag
     1021 ccatggacgg acccgcacaa tgcggacccg gcctaccggc gcgtccgggc gcgcgctctg
     1081 atccgggcct tcgaggccga gctcgggccg gggatcgcca agtcgctggc ccgcaccgcc
     1141 gacctgtgcc gcgaagacgg ggagctcctc gacgcccagg ccgaggcggc gcgtgcgggg
     1201 ctcggcgcgg tgccggtggc ggtcgccgac ctcgctgcgc ttccccgtcc gatcaggacg
     1261 cgggtatggc gcgtgctcgc caccgaggtc ggctcaccgg cggggtcgct cacggcctgc
     1321 cacgtcgagt cgctcgacac cctcgtcacg acctggcacg ggcaggggcc gctgcacctg
     1381 cccggccacg tccaggcccg ccgggtcggc ggcagggtcc gcatcgaggg cccgggtccg
     1441 gttgaatagc gacatcaccg atcgggcgcc gcccggctga cctgcacctc gcaaggagct
     1501 gtcgtggacg cgaccgacat gggacccgac ctggagaagg tcctgctcac cgaggagcag
     1561 atcctcgccc ggctggacga gatggcggcc cagatctgcg cggactacca gggcaaggac
     1621 ctgctgctcg tcggtgtcct caagggggcg gtcatcgtca tggccgacct catgcgggcc
     1681 ctgcccgtgt cggtgccgat ggactggatg gcgatctcgt cctacggctc gggcacgaag
     1741 tcctccggtg tggtgcggat catcatggac ctgcgcgacg acatcaccga ccggcacgtc
     1801 ctcatcgtcg aggacatcat cgactcgggc ctgaccctga actggatccg caacaacctg
     1861 ctctcgcgca acccggcctc gctggagatc tgcaccctgc tgcgcaagcc cgacgtggcc
     1921 aaggtcgaca tcgacgtcag gtacgtcggg ttcgacatcc ccaacgagtt cgtcgtcggc
     1981 tacggcctgg actacggcga gcgctaccgc aacctgcgcc aggtggccac cctggccccc
     2041 cacgtctact cgtaacggcg ggcccgccgg ggcgcgtcct gcccgtctcc ggcgtctccc
     2101 gccgaagtag ggaaacgtcc gcctatatcg cacgttccgc acttctcaag ggcaatatgg
     2161 gcccccaccc tggat
//
LOCUS       DATAIX010000206         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_71385, whole genome shotgun sequence.
ACCESSION   DATAIX010000206 DATAIX010000000
VERSION     DATAIX010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71385"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VHM65_03110"
     CDS             complement(<1..704)
                     /locus_tag="VHM65_03110"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX2704721.1"
                     /translation="MSGLKRLRARLQATRAMRSWKRFGDARGNILAAGVAYFAFFSVF
                     PAVTLAFTVFGLVLRGNEPLRQSLTESLSTALPGVIKSSPTSSGLIEITAPSATALTI
                     TGLVGVVTLMLAGAGWLGALRAGIRAMLGVAGTPGNMLTTKLRDLGVLVGLGLALLVS
                     IVATTGAVSAAALVSQVLGEGESPVLVTAVGLLVSAAVDVGVMVLLLRVLSGVSLPWP
                     DVRQGRDTPESTRRSRT"
     gene            complement(701..1228)
                     /locus_tag="VHM65_03115"
     CDS             complement(701..1228)
                     /locus_tag="VHM65_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEX2704722.1"
                     /translation="MATRTIGVSIPVPPPYGEYLQGRRASFGDPLAPYIRAHITLLGP
                     TLVDDTDRPELTAHLAYAASQVPPFTVILRGTGSFRPVSDVVFVQVASGISSCEQLER
                     QIRRGPYEREQPFPYHPHVTVAHDVGLDELARAFEELAGFGASFDVDAFQLWEREGDG
                     TWLAVESFPLGESPS"
     gene            complement(1231..2253)
                     /gene="trpS"
                     /locus_tag="VHM65_03120"
     CDS             complement(1231..2253)
                     /gene="trpS"
                     /locus_tag="VHM65_03120"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX2704723.1"
                     /translation="MAAMSAAPARPRTLSGMQPTSDSLHLGNYLGALVNWVELQEQFD
                     TYYFIADLHALTVPTDPAILRRRTRVTAAQFVAGGVDPARSAVFCQSHLPEHAQLGWV
                     MSCITAFGEAGRMTQFKDKVAKGQNANVGLFTYPMLMAADILMYDADRVPVGEDQRQH
                     LEITRDLAQRFNQRFGPTLVVPEPYILKETAKIMDLADPASKMSSTTSSGAGLVLLSD
                     HPQQIRKKIKSAVTDTEREIRYDPEHKAGVSNLLVIESALSGVPIATLEQGYAGQGYG
                     QLKGQVAQVVVEAVEPFQKRMAELLDDPAELDRILADGAARAAEVARGTLTRVFDRVG
                     LLPARV"
BASE COUNT          401 a          818 c          906 g          366 t
ORIGIN      
        1 gttctgctcc tgcgggtgct ctcgggcgtg tccctgccct ggcggacgtc cggccagggc
       61 agggacacgc ccgagagcac ccgcaggagc agaaccatga ctccgacgtc gacggcggcg
      121 ctgacgagca ggcccacggc cgtgacgaga acggggctct caccctcgcc gaggacttgc
      181 gacaccagcg ccgccgccga gacggcaccg gtggtagcga cgatggacac gagcagcgcc
      241 aggccgagcc cgacgaggac gccgaggtcg cgcagcttgg tcgtcagcat gttccctggg
      301 gtcccggcga cacccagcat ggcccggatc ccagcccgca gggcgcccag ccatccggct
      361 ccggcgagca tcagggtgac gaccccgacg agcccggtga tcgtcagcgc ggtcgccgac
      421 ggtgccgtga tctcgatcag cccggacgag gtcggcgagc tcttgatgac ccccggaagt
      481 gccgtactga gcgactcggt gagactctgg cgcagcggct cgttgccgcg cagcacgaga
      541 ccgaagacgg tgaacgccag ggtgacggcc gggaagaccg agaagaaggc gaagtaggcc
      601 acaccggccg cgaggatgtt gccccgcgcg tccccgaacc gcttccagct ccgcatcgca
      661 cgggtggcct gaagccgggc cctgagccgt ttgaggccgc tcacgacggc gactccccca
      721 gcgggaagga ctcgactgcc agccacgtgc cgtccccttc tcgctcccag agctggaagg
      781 catccacgtc gaaggacgcc ccgaacccgg cgagctcctc gaaggcccgc gccagctcgt
      841 ccagccccac gtcatgggca accgtgacgt gcgggtggta agggaacggc tgctcgcgct
      901 cgtagggtcc tcgtcggatc tgccgttcga gctgctcaca ggacgagatt ccgctcgcca
      961 cctggacgaa cacgacgtcc gacaccggcc tgaacgaccc cgtgccccgc aggatcacgg
     1021 tgaacggcgg gacctggctc gcggcatacg cgaggtgcgc cgtgagctcg ggacggtcgg
     1081 tgtcatcgac gagggtgggc ccgagcagcg tgatgtgagc ccggatgtat ggcgcgagcg
     1141 ggtcgccgaa cgacgcccgc ctgccctgga ggtactcgcc gtatggcggc gggacaggga
     1201 tcgagacccc gatggtccgc gttgccacgt tcagaccctc gcagggagca gcccgacccg
     1261 gtcgaacacc cgggtgagcg tgccgcgggc gacctccgcg gcccgcgccg cgccgtcggc
     1321 gaggatccgg tcgagctcgg cggggtcgtc gagcagctcg gccatccgct tctggaaggg
     1381 ctcgaccgcc tcgacgacga cctgcgcgac ctggcccttg agctggccgt acccctggcc
     1441 cgcgtagccc tgctcgaggg tggcgatggg caccccggag agggcggact cgatgacgag
     1501 caggttggac acgcccgcct tgtgctccgg gtcgtagcgg atctcccgct cggtgtcggt
     1561 gaccgccgac ttgatctttt tcctgatctg ctgggggtgg tcggacagca ggacgagccc
     1621 ggcgccggac gacgtcgtgc tggacatctt cgaggcgggg tcggccaggt ccatgatctt
     1681 ggccgtctcc ttgaggatgt acggctcggg cacgacgagg gtcgggccga agcgctggtt
     1741 gaaccgctgg gcgaggtcgc gggtgatctc gaggtgctgg cgctggtcct ctccgacggg
     1801 gacccggtcg gcgtcataca tgaggatgtc ggccgccatg agcatcgggt aggtgaacag
     1861 cccgacgttg gcgttctgac ccttggcgac cttgtccttg aactgggtca tccggcctgc
     1921 ctcgccaaag gcggtgatgc acgacatgac ccagccgagc tgggcgtgct cgggcaggtg
     1981 gctctggcag aacacggccg accgtgccgg gtccacaccg ccggcgacga actgggcggc
     2041 ggtgacgcgg gtgcgtcggc gcaggatggc cgggtcggtc gggaccgtga gcgcatgcag
     2101 gtcggcgatg aagtagtacg tgtcgaactg ctcctgcagt tccacccagt tgaccagcgc
     2161 cccgaggtag ttgcccaggt gcagcgagtc ggaggtgggc tgcatccccg acaaggtccg
     2221 gggacgcgca ggcgcagcag acatagcggc cattctgtca ggtatgccgg gcccgtccgg
     2281 tccgggtcaa cgctcagcgc tcgctatggg cagcggtcag gccttggtgg cgccgagggt
     2341 gagaccggag acgaactgct tctgcaggac gaagaagatg atgagcgtcg ggagcgctac
     2401 gatgatcgac ccggcggcga cgaggttgaa gtcggtgaag aactggccgc gcaggttgtt
     2461 gagcgaactg gtcacgggga acttgtcccc t
//
LOCUS       DATAIX010000207         2939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_41413, whole genome shotgun sequence.
ACCESSION   DATAIX010000207 DATAIX010000000
VERSION     DATAIX010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41413"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VHM65_03125"
     CDS             complement(<1..732)
                     /locus_tag="VHM65_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2704724.1"
                     /translation="MSPPDLDSLTPSDLRSRRTLKWTTYDADVLPLWVAEMDYPTAPP
                     ILEAITRAVRDETLGYPSSILERELGDAVAAWSMQRFGWAVDPFRVHSAGNVMHGLRL
                     ALTYYSAPSDPVILTTPAYPPFFDVVALARRPQVHVPMTRAAEGRPTLDLPAIDAAFG
                     AGARTLVLCHPHNPLGRVFTRAEPSDLAEVVAGHGGRVVSDEIHGSLVYDDAAYVPYA
                     AISPQTAAHTVTVSAASKAWNLPGLM"
     gene            774..1865
                     /locus_tag="VHM65_03130"
     CDS             774..1865
                     /locus_tag="VHM65_03130"
                     /inference="COORDINATES: protein motif:HMM:NF021466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HEX2704725.1"
                     /translation="MARPHASSIVEDAWHRQLDRALRRRGWKNRIIPFTGYGSLDFVR
                     VFGRVLLRRPDDPDGPDDFEDITYGGLRSPYVTRRGWRSFLTAPAMGVPITVTVGHHV
                     AHGRSDRSGHIDTTFRGHGLAPGWGTVTIAAQHAEPVEAPIFVVGPDVRFGIVSDIDD
                     TVISTSLPRPMIAAWNTFVKHESARRVVPGMAPLYRELLTEHPGAPIAFVSTGAWNTA
                     PTLARFLKTGGFPLGPLLLTDWGPTNTGWFRSGQDHKRACLDRLARDFPAIQWLLIGD
                     DGQHDPKIYQDFAEARPDRVDAIAIRQLTPTEMVLSHGLPVSNEELVARQRPPREMPV
                     VYAPDGYGLARLLRASGRLSGPDHADGHA"
     gene            1896..2756
                     /locus_tag="VHM65_03135"
     CDS             1896..2756
                     /locus_tag="VHM65_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX2704726.1"
                     /translation="MPELPEVQGLVDFLAGRVVGFAVTGVELGSISVLKTFDPPPSAL
                     TGAPIDAVTRHGKFLDVDIDGIHLVFHLARAGWLRWYDVAPRAVLRPGKSPIALRVRL
                     SDGSGFDLTEAGTKKSLAAYLVRDPALVPGVARLGPDPLAVDFTLEQFAGLLAGRRTQ
                     VKGLLRDQAFLAGVGNAYSDEVLHAAKLSPFGLAASLKPAEVERLYAALRSTLTGAIA
                     AAAGKPTNELKDAKRAGLRVHARTGQPCPECGDTVREVSFADSSLQYCATCQTGGKPL
                     ADRRMSRLLK"
BASE COUNT          402 a         1079 c         1025 g          433 t
ORIGIN      
        1 catgagtcct gggaggttcc acgccttgct cgcggcgctc accgtgaccg tgtgcgcggc
       61 cgtctgcggc gagattgccg catacgggac gtaggccgcg tcgtcataca cgagcgagcc
      121 gtggatctcg tcggagacga cccgcccgcc gtggcccgcg acgacctcgg cgaggtcgct
      181 gggctccgcg cgggtgaaga cccgcccgag cgggttgtgc gggtggcaca gcacgagggt
      241 gcgcgccccg gccccaaagg ctgcgtcgat cgccgggagg tcgagggtag ggcgcccctc
      301 cgctgcgcgg gtcatcggca catggacctg gggccgccgg gccagcgcga cgacgtcgaa
      361 gaacggcggg taggccggcg tcgtgaggat aaccgggtcg gacggggccg agtagtaggt
      421 gagggcgagc cgcagcccat gcatgacgtt gccggccgag tgcacccgga acgggtccac
      481 tgcccagccg aagcgctgca tcgaccaggc cgcgacagcg tccccgagct cgcgctcgag
      541 gatgcttgac gggtagccga gggtctcgtc gcggaccgcc cgggtgatcg cctcgaggat
      601 gggcggcgcc gtggggtagt ccatctcggc cacccacagg ggcaggacgt ccgcgtcgta
      661 cgtcgtccac ttgagggtgc gccgggaccg caggtccgat ggggtgagcg agtcgagatc
      721 cggtggcgac atggcaccat tgtggctggg gcggcggtcg gtagattgac ctcatggccc
      781 gcccccacgc gtcgtcgatc gtcgaggatg cctggcatcg acagctcgac cgggcgctgc
      841 gccggcgcgg ctggaagaac cggatcatcc cgttcacggg ttacgggtcg ctcgacttcg
      901 tccgggtgtt cggccgggtc ctgctgcgcc ggcccgacga ccccgacggt cccgacgact
      961 tcgaggacat cacgtacggc gggctgcgct cgccctacgt gacgcggcgg ggctggcgct
     1021 cgttcctcac cgccccggcc atgggggtgc cgatcacggt gacggtcggg catcacgtcg
     1081 cccatgggcg aagcgaccga agcgggcaca tcgacacgac gttccgcggc cacgggctgg
     1141 cgccgggatg gggcacggtg acgatcgccg cccagcacgc cgagccggtc gaggccccga
     1201 tcttcgtcgt cggccccgac gtgaggttcg ggatcgtcag cgacatcgac gacaccgtga
     1261 tctcgacctc gctaccacgc ccgatgatcg cggcctggaa caccttcgtc aaacacgaga
     1321 gcgcccgccg cgtcgtgccc ggtatggcgc ccctctaccg cgagctgctc accgagcatc
     1381 cgggcgcgcc catcgccttc gtctcgaccg gggcgtggaa caccgccccg accctggcgc
     1441 gcttcctcaa gaccggcggc ttcccgctgg gcccgctgct gctcaccgac tgggggccca
     1501 cgaacacggg ctggttccga agcggccagg accacaagcg cgcgtgcctc gaccggctcg
     1561 cccgcgactt ccctgcgatc cagtggctgc tcatcggcga cgacggacag cacgacccca
     1621 agatctacca ggacttcgcc gaggcccgtc ccgaccgcgt cgacgccatc gcgatccgcc
     1681 agctcacccc gaccgagatg gtgctctcgc acggtctgcc cgtctccaac gaggagctcg
     1741 tggcccgcca gcgccctccc cgcgagatgc cagttgtcta cgcgccggac ggctacggtc
     1801 tggcccggct gctgcgggcc tccgggcggc tcagcgggcc cgaccatgcc gatggccatg
     1861 cctgaggtcg atggccatgc ctgaggtcga tggccatgcc tgagctgccc gaggtccagg
     1921 ggctcgtcga cttcctcgcg ggccgcgtcg tcggattcgc ggtcaccggg gtcgagctcg
     1981 ggtcgatctc ggtgctcaag accttcgacc cgccgccgtc ggccctcacc ggagcgccca
     2041 tcgacgccgt cacgcggcac ggcaagttcc tcgacgtcga catcgatggc atccaccttg
     2101 tcttccacct cgcccgggcc ggctggctgc gctggtatga cgtcgctccc agggccgtcc
     2161 tgcggccggg caagtcaccc atcgccctgc gggtcaggct ctcggacggg tccgggttcg
     2221 acctcaccga ggccgggacg aagaagtcgc tcgcggccta cctcgtccgc gacccggcgc
     2281 tcgtgccggg cgtcgcgcgg ctgggcccgg acccgctggc cgtcgacttc accctcgagc
     2341 agttcgccgg gctgctcgcc ggccggcgga cccaggtcaa ggggctgctg cgcgaccagg
     2401 cgttcctcgc cggggtcggc aacgcctact ccgacgaggt gctgcacgcg gccaagctgt
     2461 cgcccttcgg tttggccgca tcgctcaagc cggccgaggt ggagcggctc tatgcggccc
     2521 tgcgctcgac cctgaccggg gccatcgcgg cggctgcggg caagcccacg aacgagctca
     2581 aggacgccaa gcgcgccggg ttgcgggttc atgccaggac cggccagccg tgtcccgagt
     2641 gcggcgacac ggtgcgcgag gtgtccttcg ccgactcgtc gctgcagtac tgcgcgacct
     2701 gccagaccgg cggcaagccc ctggctgacc ggcggatgtc gcgcctgctc aagtagcctc
     2761 gcccccgtga ggatcctctc gatccagtcg cacgtcgcct tcggccatgc cggcaactcg
     2821 gcggcggtct tcccgctgca ccggctcggg cacgacgtca taccggtgct caccgtcacc
     2881 ttctccaacc acaccgggta tggcgcgacg cgcggcccgc tgatcgcccc cgcagacgt
//
LOCUS       DATAIX010000208         1568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_100895, whole genome shotgun sequence.
ACCESSION   DATAIX010000208 DATAIX010000000
VERSION     DATAIX010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100895"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VHM65_03140"
     CDS             <1..481
                     /locus_tag="VHM65_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002068740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2704727.1"
                     /translation="AVLTEGGLAGLTAQWGVDDVLLDTAGPAEVDARIRLAMTRLNME
                     SQDLDALITAGDVTIDEVSYSAKVRGRVLDLTYKEFELLKYLAQHPGRVFTRSQLLHE
                     VWGYDYYGGTRTVDVHVRRLRAKLGPDNESLIGTVRNVGYRFVTERPREESADLTPV"
     gene            478..1398
                     /gene="mshD"
                     /locus_tag="VHM65_03145"
     CDS             478..1398
                     /gene="mshD"
                     /locus_tag="VHM65_03145"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: protein motif:HMM:TIGR03448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HEX2704728.1"
                     /translation="MTSRLVRSTGPLAPDVREQVVALAVRATLADGTPPLSEQFRLGV
                     AAGPLVSHLVALDQDSGAAVGYAQVDAPHAQRPAAELVVDPGRRRAGIGTLLLNALPA
                     ATHVWAHGDVPAATAFAGARGLTVVRELHRMVLDLAAATLPALPAVPGLLLRPFEPGR
                     DEDEWLRVNARAFASHPEQGALTRTDLGLRMAEPWFEPAGLLLLVPEGAPDRIAAFHW
                     TKIADPGRADGEVYVVGVDPAYQGHGLGRAATLAGLHHLRERGVRHVGLYVDGDNAPA
                     LGTYRSLGFSADGTDRMFRRDGADTVVRNP"
BASE COUNT          209 a          580 c          559 g          220 t
ORIGIN      
        1 cgccgtgctc accgagggtg gcctcgccgg gctcaccgcg cagtggggcg tcgacgacgt
       61 cctgctcgac accgccgggc ccgccgaggt cgacgcccgg atccggctcg cgatgacccg
      121 gctcaacatg gagagccagg acctcgacgc gctcatcacc gccggagacg tcacgatcga
      181 cgaggtgtcc tactcggcca aggtgcgagg gcgggtcctg gacctcacct acaaggagtt
      241 cgagctgctc aagtacctcg ctcagcatcc ggggcgggtc ttcaccaggt cccagctgct
      301 ccatgaggtc tggggttacg actactacgg cggcacccgc accgtcgacg tccacgtccg
      361 gcggttgcgc gccaagctcg gaccggacaa cgagtccctc atcgggacgg tccgcaacgt
      421 cggctaccgc ttcgtcaccg agcgtccccg cgaggagtcc gcggacctca cgccggtatg
      481 acgtcgcgtc tggtccgcag cacgggcccc ctcgcgccgg acgtgcgcga gcaggtggtc
      541 gcgctcgccg tccgggcgac gctcgcggac gggacacccc cgctgtccga gcagttccgg
      601 ctcggtgtcg cggccgggcc cctcgtgtcc catctcgtcg ccctcgacca ggactccggc
      661 gcggcggtcg ggtatgccca ggtcgatgcg ccgcacgcgc agcggccggc ggccgagctg
      721 gtggtggacc cgggccggcg tcgcgccggc atcgggaccc tgctgctcaa cgcgctgccc
      781 gcggcgaccc acgtgtgggc ccacggtgac gtgccggccg caaccgcctt tgccggcgca
      841 cgggggctga ccgtggtccg cgagctgcac cggatggtcc tggacctggc cgcggcgacc
      901 ctgcctgccc tgccggccgt cccgggcctg ctgctgcggc cgttcgagcc ggggcgggac
      961 gaggacgagt ggctccgggt caacgcccgc gcgttcgcct cccaccctga gcagggcgct
     1021 ctgacccgca cggacctcgg cctgcggatg gccgagccgt ggttcgagcc cgccgggttg
     1081 ctcctcctcg tccccgaggg agcgccggac cgcattgcgg ccttccactg gaccaagatc
     1141 gcggacccgg gccgcgcgga cggggaggtc tacgtcgtcg gcgtggaccc ggcataccag
     1201 ggtcatgggc tcggtcgcgc ggccaccctc gcgggcctgc accacctgcg ggagcggggt
     1261 gtgcgccacg tcgggctcta tgtcgacggc gacaacgcgc ccgcgctggg gacctaccgg
     1321 tcgctgggtt tctcggctga cgggaccgat cggatgttcc gtcgtgacgg cgccgacacg
     1381 gtcgtcagaa acccgtgaaa ccgggacttt ccgcccgcaa cgccgacacg atgtggtgac
     1441 agaatgaccc atgagcacgg ctgagaccat gatcgccgag caggccgagg ccgccggcgt
     1501 gagcaccagc gcgacctcga gccgcgagct cacgactctg gaggggcctg tccgctcgag
     1561 ggtgagcg
//
LOCUS       DATAIX010000209         1966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_100903, whole genome shotgun sequence.
ACCESSION   DATAIX010000209 DATAIX010000000
VERSION     DATAIX010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100903"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1240
                     /locus_tag="VHM65_03150"
     CDS             155..1240
                     /locus_tag="VHM65_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX2704729.1"
                     /translation="MPTPLARADLLSRVQRVVDSELTRQGQVLAQIGPDMEPLVDLVA
                     SLLQGGKRLRAACAYWGYRAVGGSDGEPIVRLATAMEFFQAAALLHDDVMDDSDTRRG
                     QPAAHRSMASRHTAGGWDGDSDRFGQGAAILAGNLCLTWTDALYATSGLPGEELRRAR
                     GTFDLMRTQLMGGQYLDLLGAARPWKTSSAAERIAQARRVIRFKSAKYTVEHPLLVGA
                     LAGGAGPADLAALSDYGLALGEAFQLRDDLLGVFGDPSATGKPAGDDLREGKRTVLIA
                     EFLDAADAGPRALLGERLGRPDLSSTQIDELRDVIRGSGAVERVEARIEVGAQQARVA
                     LCRTERLDPEALAVLDELVEAATIREA"
     gene            complement(1246..1647)
                     /locus_tag="VHM65_03155"
     CDS             complement(1246..1647)
                     /locus_tag="VHM65_03155"
                     /inference="COORDINATES: protein motif:HMM:NF037332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2175c family DNA-binding protein"
                     /protein_id="HEX2704730.1"
                     /translation="MPVVSHEPLSDDADPPLIADLVQEWVTVPDLCDRWRVTPARVRQ
                     LIADRELLGWRVGERNVLAVPAKLTTDAGPRPDLKGTMTVLADGGMDDEEILRWLFTP
                     DGTLPVPGAPIDALIAGHKTEVRRRAQTTAL"
BASE COUNT          302 a          707 c          686 g          271 t
ORIGIN      
        1 gtgccatggg tcatgaggcc agggtacggg cgcccgaagg gccgcggccg aacggctagg
       61 ctcgggtgag accgtctgcc accacctctc gacaccatgc cctctcgaca ccctgccctc
      121 acaacgcctt gcgacgcccg acccggagga gcccgtgccc accccgctcg cgcgtgccga
      181 cctgctcagc cgggtccagc gcgtcgtgga cagcgagctc accaggcagg gacaggtgct
      241 cgcgcagatc ggtcccgaca tggagccgct ggtcgacctc gtcgcgagcc tgctccaagg
      301 cggcaagcgc ctgcgggcgg cctgcgccta ctggggctac cgtgccgtcg gcgggtccga
      361 tggcgagccg atcgtgcgcc tggccaccgc catggagttc ttccaggccg ccgcgctgct
      421 gcacgacgat gtcatggacg acagcgacac gcgtcggggc cagccggcgg cccatcggag
      481 tatggcgtcg cgccatacgg cgggtgggtg ggacggcgac agcgaccgct tcgggcaagg
      541 tgcggcgatc ctcgccggca acctgtgcct gacctggacc gacgccctct atgcgaccag
      601 cgggctgccc ggggaggagc tgcggcgggc gcgggggacg ttcgacctca tgcggaccca
      661 gctcatgggc ggccagtacc tcgacctgct cggggccgcc cgcccctgga agaccagctc
      721 ggccgcggag cggatcgccc aggcccggag ggtcattcgt ttcaagagcg ccaagtacac
      781 cgtcgaacat ccgctgctcg tcggagccct ggctggtgga gcgggccctg ccgacctggc
      841 ggctctctcg gactacggct tggccctcgg ggaggccttc cagctgcgcg acgacctgct
      901 cggggtcttc ggcgacccca gcgccaccgg caagcccgcg ggtgacgacc tgcgtgaggg
      961 caagcgcacg gtgctcattg cggagttcct cgacgcggcg gacgccggcc cgcgagcgct
     1021 gctcggggaa cggctcggcc gccccgatct cagctcgacc cagatcgacg agctgcgcga
     1081 cgtgatccgc ggatcagggg ccgtcgagcg ggtcgaggcc cgcatcgagg tcggcgcgca
     1141 gcaggcccgg gtcgcgctct gccgcaccga gcgccttgat cccgaagcct tggccgtcct
     1201 cgacgagctg gtcgaggccg ccaccatccg cgaggcctga ccgcgctaga gggccgtggt
     1261 ctgcgcgcgg cggcggacct cggtcttgtg cccggcgatg agggcatcga tcggagcacc
     1321 cggcacgggc agcgtcccgt ccggcgtgaa gagccagcgc aggatctcct cgtcgtccat
     1381 cccaccgtcg gcgagaaccg tcatggtccc cttgaggtca gggcgtggac cggcgtcggt
     1441 ggtcagcttg gcagggacgg cgagcacgtt gcgctcaccg acccgccagc caagcagctc
     1501 acggtcggcg atgagctgac gcacccgtgc cggtgtcacg cgccagcggt cgcacaggtc
     1561 gggaacggtc acccactcct gcacgagatc ggcaatgagc ggcgggtcgg cgtcgtcgga
     1621 gaggggctca tgggagacaa ctggcacggt cctagcctgc caggtgagag cacccggccg
     1681 cgagacgtgc gggggcaacc attaattaca gcagggtaac tcaatgatat cggcgcaaac
     1741 acaggtagat ttcacaccag tccctcacgc ctcgcaaccc cccgtgtgca tcacgcatca
     1801 caggtggggg ttgccctcgc ctacccagga ggccaccagc acatgcctgc cagcatcctc
     1861 atcgccttgt ccccgccggc cgcaccagcg ggtatccgga acctggcggc cgcgccaagc
     1921 ggctggcagc gctacgccat ccgtcaggga gacaccctct gggacc
//
LOCUS       DATAIX010000210         1784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_124196, whole genome shotgun sequence.
ACCESSION   DATAIX010000210 DATAIX010000000
VERSION     DATAIX010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124196"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..693
                     /locus_tag="VHM65_03160"
     CDS             265..693
                     /locus_tag="VHM65_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX2704731.1"
                     /translation="MRISDVIRGKTPGVVTVRPDDTVTRLLAVLAEHRIGAIVVSEDG
                     SDVAGIVSERDVVRRLDRDGAGILEGPVSAIMTTKVITCEPADSVETLAGVMTDQRIR
                     HVPVLADGSLYAIVSIGDIVKHRMGALKAERDHLVGYIQQ"
     gene            818..>1784
                     /locus_tag="VHM65_03165"
     CDS             818..>1784
                     /locus_tag="VHM65_03165"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX2704732.1"
                     /translation="MTAPGSAEDGIVVSAPGTSRLGPYRLISPLGEGGMGLVHLALDP
                     NGRAVALKVLRAHVAADADARRRMEREFDTLSRIRDPRVAAVIDADVFGDRPYLVTRY
                     IPGRSLDEVVSTSGPLRGPDLLRLGRGLAGALAAIHAVGVVHRDLKPANVLIEDGEPV
                     VIDFGIAHLLDDSRITMTGLVMGTPGYLSPEVVQGGAVTEATDWWGFAATLAYAASGN
                     PPFGRGPMAGVLDRVRCGEFDLSGVDPRLAPLLAAALAPDASRRPHHAAVVAALEQYA
                     RGDAVTVALGPPAAGDATGPVASYPETRPMPVQPTREWTGPAWAGQ"
BASE COUNT          224 a          628 c          667 g          265 t
ORIGIN      
        1 ccaggcaccc tcgtgggctg ccatgaccga gcccatggcg gtgacgaggc ccccggggct
       61 gcgccgccac gtgatcgtgc cctcgttgtc gacatgccgg tccacgggca ggcggttggc
      121 gacgatgacg aagtcaaccg ccgggtaggg ctgggactgc tcgttcacgt caaacctccc
      181 tccccccacc ataggcgtgc ggggaggctg atagcggccg ggcgaggtat ggcggcgcgg
      241 ggctcccggg gttaccttcg gactatgcgg atctcagacg tcatacgcgg caagaccccc
      301 ggagtcgtca ccgtgcggcc cgacgacact gtgacccgac tgctcgccgt gctggcggaa
      361 caccggatcg gcgccatcgt cgtcagcgag gacggcagcg acgtcgcggg gatcgtcagc
      421 gaacgggatg tcgtgcgaag gctcgaccgc gacggagcgg ggatcctcga gggcccggtc
      481 agtgcgatca tgaccacgaa ggtgatcacc tgcgagccgg ccgactcggt cgagacgctc
      541 gcgggagtca tgaccgacca gcgcatcagg cacgtcccgg tcctggccga cgggtcgctc
      601 tatgccatcg tgagcatcgg tgacatcgtc aagcatcgga tgggcgccct gaaggccgag
      661 cgcgaccacc tcgtcgggta catccagcag taggccctcc ggcggctccg cccaggtggg
      721 ccggccgcca taccggctcg gccgtctggc ccggctcgcc cgtctggccc ggcggtccgg
      781 ccctcccgac tgcgcgcggt cgcttaccgt tgtcggtatg acggcgcccg gctccgcgga
      841 ggacggcatc gtcgtgtcag cgcccggcac gtcgcggctc gggccgtatc gcctgatctc
      901 gccgctcggt gagggcggga tggggctcgt ccacctcgcg ctggacccga acggccgggc
      961 cgtcgcgctc aaggtcctgc gcgcccacgt ggcggctgac gccgacgcgc ggcggcggat
     1021 ggagcgggag ttcgacacgc tgtcgcggat ccgggacccc cgggtggcgg cggtcatcga
     1081 cgcggacgtg ttcggggacc ggccctatct cgtcactcgg tacatccccg gtcggtccct
     1141 cgatgaggtc gtgagcacgt cggggccgct gcgcggcccg gacctgctgc gcctgggtcg
     1201 cggcctggcc ggggcgctcg ccgcgatcca tgcggtgggc gtcgtgcatc gtgacctcaa
     1261 gccggccaac gtcctgatcg aggacgggga gccggtcgtc atcgacttcg gcatcgcgca
     1321 cctgctcgac gactcgcgga tcacgatgac cggcctcgtg atggggacgc cggggtacct
     1381 ctcgcccgag gtcgtccagg gcggcgcggt caccgaggcg accgactggt ggggcttcgc
     1441 cgcgaccctt gcgtatgccg cgtcggggaa cccgccgttc gggcgcgggc ccatggcggg
     1501 cgtgctcgac cgggtccggt gcggggagtt cgacctttcg ggtgtcgacc cgcggctggc
     1561 tccgctgctt gcggcggctc tggccccgga cgcgtcccgg cgcccgcacc acgccgcggt
     1621 ggtcgcggcg ctggagcagt acgcccgtgg tgacgcggtc acggtggcgc tcgggcctcc
     1681 cgcggccggc gacgcgaccg gaccggtggc gtcatacccg gagactcgtc cgatgcctgt
     1741 ccagcccacg cgggagtgga ccggtcccgc gtgggctgga cagg
//
LOCUS       DATAIX010000211         2980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_160990, whole genome shotgun sequence.
ACCESSION   DATAIX010000211 DATAIX010000000
VERSION     DATAIX010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160990"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VHM65_03170"
     CDS             <1..944
                     /locus_tag="VHM65_03170"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX2704733.1"
                     /translation="IRTTATMGPAYTELHSMVKRDGLHTVCQEAGCPNIFECWEDREA
                     TFLIGGEQCTRRCDFCQIETGRPASLDRDEPRRVAESVRQMGLRYSTITGVARDDLPD
                     GGAWLYAETIRQVHALNPGTGVEILVPDFNAVPALVAEVIDTRPEVWAHNLETVPRVF
                     RRIRPAFGYDRSLRVITMGRDAGLVTKSNLILGMGERGEEIEAALRDLYDAGTDIVTI
                     TQYLRPSPRHHPIDRWVTPEEFVHWSDVALALGFKGAMAGPLVRSSYRAGRLWATAMR
                     RWGRAIPDQLRHLAEAAGSPARQEAAALVARSAIDVS"
     gene            950..1720
                     /locus_tag="VHM65_03175"
     CDS             950..1720
                     /locus_tag="VHM65_03175"
                     /inference="COORDINATES: protein motif:HMM:NF025199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4191 domain-containing protein"
                     /protein_id="HEX2704734.1"
                     /translation="MAKDSPATAPAKRSLFSRRSARGPAKGPGRLAQVRQVYTSSRAV
                     DPAITWWMLGASLGILAIALVIGLLTHAPVYALVLGLPMALLAATIVLSRRAERAAYR
                     QIEGQPGAAGAALSSLRRGWFVEEQPVAADAARPGDVAHAAIVFRALGRPGIVLVGEG
                     PSARAQRLLIAERKKVERVAPGVPVTTLRIGDGGGTDEIAIRKLTSHVQRMKPVLTKD
                     EVITVNKRLRALGGLRTAIPAGIDPTKVRMDRKAMRGR"
     gene            1814..>2980
                     /locus_tag="VHM65_03180"
     CDS             1814..>2980
                     /locus_tag="VHM65_03180"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2704735.1"
                     /translation="MTFLRDLRILLAYGGFRRLLGVRLTSQSSDGLFQAALATLLLFN
                     PERAPSAAAIAGGFAVLLLPFSVLGPFAGVFLDRWPRRAVLVTTNLVRALLLVGLAVL
                     KVNDQVGGLFVVLVLAVLGLNRFLLAGLSASLPHVVDRDKLVTANAVTPTCGTLAYLV
                     GIVIGGVLAEQAPGYTPFVAAAGGYLAAALIARTMPDLGPDLAAADASVRAAVRHMLS
                     GISDAATHLPRTAQVALGLVTAIRIPVGIMIVATILYFRAAHPPGASGVIGFGSAVVA
                     TGVGFAVAAVATPALTRRFGVPRTVRLLATASGAVQLLALPFAPVLLVAAAFGLGATT
                     QSIKICIDTILQRDVSDAFRGRVFALYDILFNVAFVAATAIAALVLPPTGRSAWV"
BASE COUNT          370 a         1144 c         1009 g          457 t
ORIGIN      
        1 ggatccgcac gacggcgacc atgggcccgg cttacaccga gctgcactcg atggtgaagc
       61 gcgacgggct gcacacggtc tgccaggagg ccgggtgccc gaacatcttc gagtgctggg
      121 aggaccgcga ggcgaccttc ctcatcggcg gcgagcagtg cacccgccgg tgcgacttct
      181 gccagatcga gaccggccgg ccagccagcc tggatcggga cgagccacgg cgggtcgccg
      241 agtcggtgcg ccagatgggg ctgcgctact cgacgatcac cggcgtcgcc cgggacgacc
      301 ttcccgacgg aggcgcctgg ctgtatgccg agacgatccg tcaggtgcac gcgctcaacc
      361 ccggcacggg ggtggagatc ctcgtccctg acttcaacgc cgtccccgct ctcgtggccg
      421 aggtcatcga cacccgcccc gaggtgtggg cccacaacct cgagaccgtc ccccgggtct
      481 tccggcgcat ccggcccgcc ttcggctacg accgctcact gagggtcatc accatgggcc
      541 gcgacgcggg gctggtcacc aagagcaacc tcatcctcgg gatgggcgag cggggcgagg
      601 agatcgaggc agccctgcgg gacctgtatg acgcgggcac ggacatcgtg acgatcaccc
      661 agtacctccg gccgtccccg cgacaccacc ccatcgaccg gtgggtcacg cccgaggagt
      721 tcgtccactg gtccgacgtc gccctggcgc tcggcttcaa gggcgccatg gccggacccc
      781 tggtgcgctc ctcctatcgg gccgggcggc tctgggccac cgcgatgcga cggtggggac
      841 gcgccatacc ggaccagctg cgtcacctgg ccgaggcggc cggctcgccc gcccgccagg
      901 aagcggccgc attggtcgcg cggtccgcca tcgacgtatc ctgaccctca tggccaagga
      961 ctcccccgcg acggcgcccg cgaagcgctc gctcttctcc cgccgctcgg ctcggggccc
     1021 ggccaaaggc ccgggccggc tcgcccaggt ccgccaggtc tacacgtcgt ccagggcggt
     1081 cgaccccgcc atcacctggt ggatgctcgg cgcctccctc ggcatcctcg ccatcgcgct
     1141 cgtcatcggt ctgctcaccc acgccccggt ctatgcactg gtcctgggtc tgcccatggc
     1201 cctgctcgcc gcgacgatcg tcctgagccg ccgggccgag cgcgcggcat accggcagat
     1261 cgagggccag ccgggagcgg ccggcgcggc cctgtcctcg ctgcgccgtg gctggttcgt
     1321 cgaggagcag ccggtggccg ccgacgccgc ccggcccggt gatgtcgccc atgccgccat
     1381 cgtctttcgc gccctcggcc gccccgggat cgtgctcgtc ggcgagggcc cctcggcccg
     1441 cgcccagcgc ctcctcatcg ccgagcgcaa gaaggtcgag cgggtggccc ccggagtccc
     1501 cgtcacgacc ctgcgtatcg gtgatggtgg ggggacggac gagatcgcga tccgcaagct
     1561 gacgagccac gtccagcgga tgaagcccgt ccttaccaag gacgaggtca tcacggtcaa
     1621 caagcggctg cgggccctcg gcgggctacg caccgccatc cccgcgggca tcgacccgac
     1681 caaggtccgc atggaccgca aggcgatgcg cggccgctga ctccagcgcg gtcgcggcat
     1741 acgtcatacc gaccaaggag gagctcgaga tcgcccggca gtgcctggcc gtcgtggcac
     1801 ctacgctgag cccgtgacct tcctgcgcga cctgcgcatc ctcctcgcgt atggcggctt
     1861 ccggcgcctg ctcggggtgc ggctgacctc gcagtcctcc gacgggctgt tccaggcggc
     1921 gctggccacc ctgctcttgt tcaacccgga gagggccccg tcggcggcgg cgatcgcggg
     1981 cgggttcgcg gtcctgctgc tcccgttcag cgtgctcggc ccgttcgccg gagtcttcct
     2041 cgaccggtgg ccgcgccggg ccgtcctggt caccaccaac ctcgtccggg cgctgctgct
     2101 cgtcgggctg gcggtcctca aggtcaacga ccaggtgggc gggctcttcg tcgtcctcgt
     2161 tcttgcggtc ctcgggctca accggttcct cctcgccggg ctgtccgcct ccctccccca
     2221 cgtcgtggac cgcgacaagc tcgtgacggc caacgcggtg accccgacgt gcggcacgct
     2281 cgcctacctc gtggggatcg tcatcggagg cgtcctggcc gaacaggcac ccggttacac
     2341 gccgttcgtg gccgcggcgg gcggctacct cgcggcggca ctgatcgcgc ggaccatgcc
     2401 tgacctcgga ccggacctgg ccgccgcgga cgccagcgtg cgggcggcgg tgcggcacat
     2461 gctctccggg atctccgacg ccgctaccca tctgccccgc acggcgcagg tggccctcgg
     2521 gctcgtcacc gcgatccgga tcccggtcgg gatcatgatc gtcgcgacga tcctctactt
     2581 ccgcgccgcc cacccaccgg gtgcgagcgg ggtgatcggc ttcggctccg cggtggtggc
     2641 gacgggcgtg gggttcgcag tggcggccgt cgctactccg gcgctgaccc gccgcttcgg
     2701 ggtcccccgg acggtccgcc tgctcgcgac cgcttcgggc gccgtccagc tgcttgccct
     2761 gcccttcgcg ccggtcctgc tcgtcgccgc cgccttcggc ctgggcgcga cgacccagag
     2821 catcaagatc tgcatcgaca ccatcttgca gcgcgacgtc agcgacgcgt tccgggggcg
     2881 ggtcttcgcc ctctacgaca tcctcttcaa cgtggcgttc gtcgcggcca cggccatcgc
     2941 cgcgctcgtg ctgccaccga ccgggcgctc ggcgtgggtt
//
LOCUS       DATAIX010000212         1638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_213549, whole genome shotgun sequence.
ACCESSION   DATAIX010000212 DATAIX010000000
VERSION     DATAIX010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213549"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..928)
                     /locus_tag="VHM65_03185"
     CDS             complement(86..928)
                     /locus_tag="VHM65_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2704736.1"
                     /translation="MTTGSSGRGSHTGHTGLQVSELSAGYGRLEVLYDIDLEVRPGEV
                     LAVVGANGAGKTTVLRAISGLLRPSHGAITLNGTDITTATAERIASLGLAHVPENRLV
                     FPSLSVADNLALGRWTRRREGDARAGTERVLALFPRLADRTAQAAGSLSGGEQQMLAI
                     GRALMAEPSVVVLDEPSLGLAPRVVGEIMAVLAHLRADRGLAVLLVEQNVRAAFTVAD
                     HVLVMDRGRVVATGTAAELAQDERVRMAYLGGSSTTSGSIAAPVSGPAAAPTAAVDGP
                     APAG"
     gene            complement(925..>1638)
                     /locus_tag="VHM65_03190"
     CDS             complement(925..>1638)
                     /locus_tag="VHM65_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2704737.1"
                     /translation="GEILALIGPNGAGKTTAFNIVTGVIAPTSGTVTLMGTPVQGRRP
                     HVVAALGATRTFQNLQVFGSTSVLGNVMVARHLRSRSGVIRGMLGLAADEHRAVESAA
                     RTALAAMGLSEQAERTVTDLSFGQQRQVEVARALALAPILLLLDEPMAGLSAVSRDAL
                     SGLLRQIRDAGIAILLVEHDVDAVMALADRVAVLDDGILIALGTPGQVANDPVVVAAY
                     LGTDEAADALVRTAHVEES"
BASE COUNT          228 a          594 c          605 g          211 t
ORIGIN      
        1 gtccaatcca accatggcgg ctcggcccgg agcccgggga accagtggcc cctcaccggg
       61 gacccaggcg cgccgagcgg gccgctcagc cggccggggc gggcccgtcg acggcagcgg
      121 tcggcgcggc ggcgggcccg ctcacgggcg cggcgatgga gccggacgtc gtcgaggacc
      181 cgccgaggta ggccatccgt acccgctcgt cctgggccag ctcggccgcc gtgcccgtcg
      241 ccacgacccg gccacgatcc atgacgagaa cgtggtcggc cacggtgaac gcggcccgga
      301 cgttctgctc gacgagcagc accgccaggc cgcggtccgc acgcaggtgg gccaggacgg
      361 ccatgatctc gccgacgact cggggcgcga ggcccaggct cggctcgtcg aggacgacga
      421 ccgatggctc ggccatgagc gcccggccga tcgcgagcat ctgttgctca ccgccggaca
      481 gtgaccccgc cgcttgtgct gtccggtcgg ccagccgcgg gaagagcgcc aggacccgct
      541 cggtgcccgc acgggcatca ccctcgcgtc tgcgggtcca ccgaccgagc gcgaggttgt
      601 cggcgacgct gagcgagggg aagacgagcc ggttctcggg cacgtgggcc aggccgaggc
      661 tcgcgatccg ctcggccgtg gcggtcgtga tgtcggtccc gttgagggtg atcgcgccat
      721 gggagggccg caggagcccg gagatcgccc gcaggacggt tgtcttgccg gccccgttcg
      781 cgccgaccac cgccagcacc tcgccgggcc tgacctccag gtcgatgtcg tacaggacct
      841 cgaggcggcc atagccggcg gacagctcgc tgacctgcag cccggtgtgc ccggtgtggc
      901 tcccacgccc ggagctcccc gtcgtcatga ctcctccaca tgggcggtgc ggacgagtgc
      961 gtcggccgcc tcgtcggtgc cgaggtacgc cgcgacgacg acggggtcgt tcgcgacctg
     1021 acccggagtc ccgagcgcga tgaggatccc gtcgtcgagc acggcgaccc ggtcggcgag
     1081 ggccatgacc gcgtcgacgt cgtgttcgac gagcaggatc gcgatccccg cgtcccggat
     1141 ctggcgcagc aggccggaga gcgcgtcccg ggagaccgcc gagagcccgg ccatgggttc
     1201 gtcgagcagg agcaggatcg gggccagtgc cagggcccgg gcgacctcca cctgacgctg
     1261 ctggccgaag gagagatcgg tgaccgtccg ctcggcctgc tcgctgagac ccatcgcggc
     1321 cagtgcggtg cgcgccgcgg actcgacggc gcggtgctca tcagcagcca gccccaacat
     1381 gccccggatc acgcccgatc ggctgcgcag gtgccgggca accatgacgt tgccgaggac
     1441 cgacgtgctc ccgaagacct gcaggttctg gaaggtgcgg gtcgcgccga gggcggccac
     1501 gacgtgcggc cgtctcccct ggaccggtgt ccccatgaga gtcacggtcc cgctcgtcgg
     1561 ggcgatgacc ccggtgacga tgttgaacgc ggtcgtcttc ccggccccgt tggggccgat
     1621 caaggccagg atctcccc
//
LOCUS       DATAIX010000213         3691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_41640, whole genome shotgun sequence.
ACCESSION   DATAIX010000213 DATAIX010000000
VERSION     DATAIX010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41640"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VHM65_03195"
     CDS             complement(<1..353)
                     /locus_tag="VHM65_03195"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEX2704738.1"
                     /translation="MAGATTTRSVCCPSRTWGTSWTSCQTSVWTGLPDSAAHVGAPTN
                     ASAAAVADFRPRTYAAAKIKKSHAREAGSPTADEPAPTVELVRNPDILAGLVQARGAA
                     HRPLIVGFAAETGDS"
     gene            250..1359
                     /gene="coaBC"
                     /locus_tag="VHM65_03200"
     CDS             250..1359
                     /gene="coaBC"
                     /locus_tag="VHM65_03200"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX2704739.1"
                     /translation="MSGKPVQTDVWHDVHEVPHVRLGQQTDLVVVAPATADLIARAAH
                     GRADDLLTATLLTARCPVVMAPAMHTEMWEHPATQANVATLAARGIHIVPPASGRLTG
                     ADTGPGRLPEPADLYDACLAVARASTPLQGMRVVISAGGTREPLDPVRFLGNRSSGKQ
                     GYALAAVAAERGADVTLVSANVALPVPAGVALVTVGTALQMQAAVTERAGSADVIVMA
                     AAVADFRPRTYAPAKIKKSHRREAGSPAGGEPAPTVELVRNPDILAGLVQARGAAHRP
                     LIVGFAAETGDSEGSVLDHARAKLERKGADLVVANEVGETRTFGQDSNEVSILRRSRP
                     EVTHVGQAPKERIAGAVWDAIQDALGVAGPTGASG"
     gene            1408..2643
                     /gene="metK"
                     /locus_tag="VHM65_03205"
     CDS             1408..2643
                     /gene="metK"
                     /locus_tag="VHM65_03205"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX2704740.1"
                     /translation="MPARLFTSESVTEGHPDKICDQISDATLDAMLVEDKHSRVAVET
                     MVTTGLVHVAGEVSTTGYVDIPSIVRETVLAIGYDSSTKGFDGQSCGVQVSIGQQSPD
                     IAQGVDTAYESRTGGVDPLDKQGAGDQGLMFGYACDDTPELMPLPIFLAHRLAERLTA
                     VRKAGRVPYLRPDGKTQVTIGYEGDRAVRLDTVVLSTQHAADISLEGLLVPDIIEHVI
                     IPVLHELTESGSDLDVSDYRTLINPTGRFEIGGPMGDAGLTGRKIIVDTYGGMARHGG
                     GAFSGKDPSKVDRSAAYATRWVAKNVVAAGLARRCEVQVAYAIGRAQPVGLYVETFGT
                     ETAPVDAIQCAITSVFDLRPAAIVDQLDLLRPIYRATAAYGHFGRISANGVEGAFTWE
                     RLDRVEALRAAVAGPAGPG"
     gene            2648..>3691
                     /locus_tag="VHM65_03210"
     CDS             2648..>3691
                     /locus_tag="VHM65_03210"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704741.1"
                     /translation="MAEPTQRSAPRVPSAGGRPVAAVVVATGLAHLDRVFEYAIPDEL
                     TDTAQPGVRVRVRFAGRDLDGFVVERRHEARHEGPLAPLRRVVSPEPVLTPDILALVR
                     AVADRYAGTRGDVLRLAVPKRHAAAERALTERPPEQVALVPTPEPGPWSAYPAGPSLV
                     RRVGRGENPAASWLALPGQRDNADWPAALAVLAVAAVSAGRGAVIVVPDHRDVDRVDS
                     ALRTALGAGRHVRLTAAQGPHARYTAWLSILRGHVQCVVGTRAAMFAPVRNLGLVAWW
                     DDGDDLLAEPRAPYPHVREVLMLRAAQSGAAAVSGGFARSVDQQLALESGGVHAVAAA
                     PAQVRATAPRVLVA"
BASE COUNT          474 a         1389 c         1313 g          515 t
ORIGIN      
        1 tcggagtcgc cggtctcggc ggcgaacccg acgatgagcg gccggtgggc ggccccgcgc
       61 gcctggacga gcccggcgag gatgtcgggg ttgcggacca gctcgacggt cggggccggc
      121 tcgtccgcgg tcggcgaacc cgcctcgcga gcgtgcgact tcttgatctt ggccgccgca
      181 taggtccggg gccggaagtc ggccaccgcg gcggcgctgg cgttcgtcgg ggcgccaacg
      241 tgggcggcgc tgtccggcaa gccggtccag accgacgtct ggcatgacgt ccacgaggtc
      301 ccccatgtcc ggctcggaca gcagaccgac ctcgtcgtcg tcgccccggc caccgctgac
      361 ctgatcgcca gagcggccca cggccgcgcc gacgacctgc tgaccgccac gctgctcacc
      421 gcgcgctgcc cggtggtcat ggcaccggcg atgcacaccg agatgtggga gcacccggcg
      481 acccaggcca acgtcgccac cctggcggcg cggggcatcc acatcgtccc acccgcctcg
      541 ggtcgcctca ccggtgccga caccggcccc ggacgcctgc ccgaacccgc ggacctgtat
      601 gacgcgtgcc ttgccgtcgc gcgcgcttcg acccccttgc aggggatgcg ggtcgtcatc
      661 tccgccggtg gcacgcgcga accgctcgac ccggtgcgct ttctcggcaa ccggtcttcg
      721 ggcaagcagg gctatgctct cgccgccgtc gccgccgagc gaggtgccga cgtgaccctc
      781 gtcagcgcca acgtcgcgtt gccggtgcct gctggtgtcg cgctcgtcac cgtggggacg
      841 gcgctgcaga tgcaggcggc cgtgaccgag cgggcgggat ccgccgatgt catcgtcatg
      901 gccgccgcgg tggccgactt ccggccccgg acctacgcgc cggccaagat caagaagtcc
      961 catcgtcgcg aggcgggttc gccggctggg ggcgagccgg ccccgacggt cgagctggtc
     1021 cgcaaccccg acatcctcgc cgggctcgtc caggcgcgcg gggccgccca ccggccgctc
     1081 atcgtcgggt tcgccgccga gaccggcgac tccgagggct ccgtcctcga ccatgcccgc
     1141 gccaagctcg agcgcaaggg cgccgacctc gtggtcgcca acgaggtggg ggagacccgc
     1201 accttcggcc aggacagcaa cgaggtgtcg atcctgcgtc gtagccgccc cgaggtcacc
     1261 cacgtcggcc aggcccccaa ggagcgcatt gctggcgccg tgtgggacgc catccaggac
     1321 gctctcgggg ttgcgggtcc caccggcgcg tcgggataga ctcggctgct ggtccacccc
     1381 ctgtcaccca acgtgcgagg agatcgcgtg cctgcccggc tgttcacgtc cgagtccgtc
     1441 accgagggtc accccgacaa gatctgcgac cagatctcgg acgcgaccct cgacgccatg
     1501 ctcgtcgagg acaagcactc ccgcgtcgcg gtcgagacca tggtcacgac cgggctcgtc
     1561 cacgtcgccg gtgaggtctc gacgaccggc tacgtcgaca tcccctcgat cgtgcgcgag
     1621 accgtgctcg cgatcggcta cgactcctcg acgaagggct tcgacggcca gtcgtgcggg
     1681 gtccaggtct cgatcgggca gcagagcccc gacatcgccc agggcgtcga cacggcctac
     1741 gagtcgcgga ccggcggggt ggacccgctg gacaagcagg gcgccggtga ccagggcctc
     1801 atgttcggct atgcctgcga cgacaccccc gagctcatgc cactgccgat cttcctcgcc
     1861 catcggctcg cggagcgcct caccgcggta cgtaaggccg ggcgcgtccc gtacctgcgt
     1921 ccggacggca agacccaggt gaccatcggc tacgagggtg accgcgccgt ccggctcgac
     1981 accgtcgtgc tctcgaccca gcacgccgcg gacatctcgc tcgagggtct gctcgtcccc
     2041 gacatcatcg agcacgtcat catcccggtc ctgcacgagc tcaccgagtc ggggtccgat
     2101 ctggacgtgt cggactaccg gactctcatc aacccgaccg gccggttcga gatcggaggg
     2161 cccatgggtg atgccgggct caccggtcgc aagatcatcg tcgacacgta tggcgggatg
     2221 gctcgccatg gtgggggagc cttctcgggc aaggacccct ccaaggtcga ccgctccgcc
     2281 gcgtatgcca cccgctgggt cgccaagaac gtcgtggctg ccgggctcgc gcgccgctgc
     2341 gaggtccagg tcgcctacgc catcggcagg gcccagcccg tgggcctgta tgtcgagacc
     2401 ttcggcaccg agaccgcgcc ggtcgatgcg atccagtgcg ccatcacgtc ggtgttcgac
     2461 ctgcgtccgg cggcgatcgt cgaccagctc gacctgttgc gcccgatcta ccgggcgacc
     2521 gccgcctacg ggcacttcgg ccggatctcg gccaacgggg tcgagggcgc cttcacgtgg
     2581 gaacgcctcg accgggtcga ggcgctccgc gcggccgtcg ccggcccggc cggcccaggc
     2641 tgaaccagtg gccgagccga cccagcgcag cgcaccccgg gtgccctcgg ccgggggccg
     2701 gccggtcgct gccgtggtcg tcgccaccgg gctggcacat ctggaccggg tcttcgagta
     2761 cgccatccct gacgagctca ccgacaccgc ccagccgggg gtacgggtca gggtccgctt
     2821 cgccggtcgc gacctcgacg ggttcgtcgt cgagcgccgc cacgaggccc gccatgaggg
     2881 cccgctcgcg ccgctacggc gggtcgtcag ccccgaaccc gtcctcaccc ctgacatcct
     2941 ggctctggtc cgcgccgtcg cggaccggta tgccggcacg cgtggcgacg tgctccgcct
     3001 cgcggtcccg aagcggcacg ccgccgccga gcgggccctg accgagcggc ctccggagca
     3061 ggtcgcgctc gtgccgaccc ccgaacccgg gccgtggtcg gcataccccg ccggtccttc
     3121 gctcgtccgg cgggtggggc ggggtgagaa cccggcggcg tcgtggctgg cactgcccgg
     3181 ccagcgcgac aacgcggact ggccggccgc tctcgcggtc ctcgcggtgg cggccgtcag
     3241 cgccgggcgc ggggcggtca tcgtcgtgcc cgaccaccgg gacgtcgacc gggtcgactc
     3301 ggctctgcgg acggccctgg gggcggggag gcacgtccgg ctcaccgcgg cacaaggccc
     3361 tcatgctcgc tacaccgcgt ggctgagcat cctgcggggg catgtgcagt gcgtcgtcgg
     3421 gaccagggcg gcgatgttcg cgcccgtgcg caacctgggc ctggtcgcct ggtgggacga
     3481 cggggacgac ctgctggccg agccccgcgc gccatacccg cacgtccggg aggtgctgat
     3541 gctgcgcgct gcgcagtcgg gggccgcagc ggtgagcggg gggttcgcgc gcagcgtgga
     3601 ccagcagctg gcgctggagt ccggcggtgt ccatgcggtg gcggcggcgc cggcccaggt
     3661 ccgggcgacc gccccccgcg tgctggtggc c
//
LOCUS       DATAIX010000214         2252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_71714, whole genome shotgun sequence.
ACCESSION   DATAIX010000214 DATAIX010000000
VERSION     DATAIX010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71714"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VHM65_03215"
     CDS             complement(<1..392)
                     /locus_tag="VHM65_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX2704742.1"
                     /translation="MSVQEFRLPDPGEGLTEAEIVSWKVRPGDIVKVNDIVVEIETAK
                     SLVELPVPFAGVVSALLAAEGETVEVGRAIIAIDDGSGGPVAPAPPPAAEASAAAPVP
                     APGSGGPAPIEQGKIGGTTSTGRTAVLVG"
     gene            complement(408..1373)
                     /locus_tag="VHM65_03220"
     CDS             complement(408..1373)
                     /locus_tag="VHM65_03220"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX2704743.1"
                     /translation="MTIAKAVNSGLRAAMERDPKVVLMGEDIGKLGGVFRVTDGLQKD
                     FGEDRVLDTPLAESGIVGTAVGMALRGYRPVVEIQFDGFVYPAFDHIVSQVAKMGARA
                     LGHVRLPIVIRIPVGGGIGAVEHHSESNEAYFAHTAGLKVVAVSNPHDAFWMVQQAIE
                     TDDPVIFYEPKRRYWDKGEVDESASRVGMFEARVVRPGTAVTVAAYGPMVKTCLQAAE
                     AAAEVGRNLEVIDLRSLSPLDLGTLATSVGRTGRLVVVHEAPTFLGMGAEIAAEVTTR
                     CFYQLEAPVVRVGGYNVPYPPSKIEEDFLPDLDRVLDGVDRALAY"
     gene            complement(1384..>2252)
                     /locus_tag="VHM65_03225"
     CDS             complement(1384..>2252)
                     /locus_tag="VHM65_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX2704744.1"
                     /translation="LRHQDYAFPTYREHGVAWVRGVNPLNLLGLFRGVNQGGWDPNEN
                     NFHLYTVVIGAQTLHAVGYAMGIQHDHAVGTGEPDRDAAVIAYFGDGATSQGDVNESF
                     VFAAVSNAPVVFFCQNNQWAISEPNERQTRIPLYQRARGFGFPGVRVDGNDVLAVYAV
                     TKHALDEARSGQGPSFIEAYTYRMGAHTTSDDPTKYRVSAEVEVWKLRDPILRLKAYL
                     AHEGKADGAFFEAVEAEADALAASVRKGCLEMPDPAGTLMFDYVYADPHPLVVAERAE
                     FLAYQASFEGAH"
BASE COUNT          337 a          773 c          795 g          347 t
ORIGIN      
        1 ccgacgagga cagcggtccg gccggtcgag gtggtgccgc cgatcttgcc ctgctcgatc
       61 ggcgcgggcc cgcccgagcc aggggcgggc accggagcag ctgccgatgc ctcggccgcg
      121 ggcggtgggg ccggggcgac cggcccgccc gagccgtcgt cgatggcgat gatcgccctg
      181 cccacctcga cggtctcgcc ctcggcggcg agcagcgccg atacgacccc cgcgaagggc
      241 acgggcagct cgacgagcga cttggccgtc tcgatctcga cgacgatgtc gttgaccttg
      301 acgatgtcgc cgggccggac cttccacgag acgatctcgg cctcggtgag accctcgccc
      361 gggtcgggca gcctgaactc ctggacactc atctgcgatc caacccctca gtacgccagc
      421 gcacggtcga caccgtcgag cacccggtcg aggtccggga ggaagtcttc ctcgatcttg
      481 ctcggcggat agggcacgtt gtagccaccg acccggacca cgggcgcctc gagctggtag
      541 aagcagcgcg tggtgacctc ggcggcgatc tcggcgccca tcccgaggaa ggtcggcgct
      601 tcgtggacga cgacaaggcg acccgtgcgc cccaccgagg tggcgagcgt gccgaggtcg
      661 agcgggctca gcgagcgcag gtcgatgacc tcgaggttcc ggcccacctc ggcggccgcc
      721 tcggccgcct gcaggcaggt cttgaccatc ggcccgtatg ccgcgaccgt caccgcagtg
      781 cctggccgga cgaccctggc ctcgaacatc cccacgcgag aagcggactc gtcaacctcc
      841 cccttgtccc aatagcggcg tttgggctcg tagaagatca ccgggtcgtc ggtctcgatg
      901 gcctgctgga ccatccagaa cgcatcatgg gggttggaca cggcgacgac cttgaggccg
      961 gccgtgtggg cgaagtaggc ctcgttggac tcgctgtggt gctcgaccgc gccgatgccg
     1021 ccaccaaccg ggatccggat cacgatgggc agccgcacgt gaccgagggc ccgggcgccc
     1081 atcttggcga cctggctgac gatgtggtcg aaggccgggt agacgaaccc gtcgaactgg
     1141 atctcgacga ccgggcggta gccgcgcagc gccataccga ccgcggtccc gacgatcccg
     1201 gactcggcga gcggggtgtc gaggacccgg tcctcgccga agtccttctg cagcccgtcg
     1261 gtgacccgga agaccccgcc gagcttgccg atgtcctcgc ccatgaggac gaccttgggg
     1321 tcgcgctcca tcgcggcgcg caggccggag ttgaccgcct tggcaatggt catggtcgcc
     1381 atgtcagtgc gccccctcga agctggcctg gtaggcgagg aactcggccc gctcggccac
     1441 gacgagcggg tgcgggtcgg cgtagacata gtcgaacatc agggtcccgg ccgggtctgg
     1501 catctccagg cagcccttgc ggaccgaggc ggcgagggcg tcggcctcgg cctcgacggc
     1561 ctcgaagaag gcaccgtcgg ccttgccctc gtgggcgaga taggccttga gccgcaggat
     1621 cgggtcgcgc agcttccaca cctcgacctc ggcgctgacg cggtacttcg tcgggtcgtc
     1681 cgaggtcgtg tgggcgccca tccggtaggt gtaggcctcg atgaaggacg gtccctgccc
     1741 gctgcgcgcc tcgtcgaggg cgtgcttggt gacggcatac accgcgagga cgtcgttgcc
     1801 gtcgacccgc acccccggga agccgaagcc gcgtgcccgc tggtagagcg ggatccgcgt
     1861 ctggcgctcg ttgggctcgg agatcgccca ctggttgttc tgacagaaga agacgaccgg
     1921 tgcgttgctg acggcggcga agacgaacga ctcgttgacg tcgccctggc tcgtcgcgcc
     1981 gtcgccgaag taggcgatga ccgctgcgtc ccggtccggt tcgccggtgc ccaccgcgtg
     2041 atcgtgctgg atgcccatcg cgtagcccac ggcgtgcagc gtctgtgcgc cgatgaccac
     2101 ggtatagagg tggaagttgt tctcgttcgg gtcccagcca ccctggttga ccccccggaa
     2161 cagcccgagc aggttgagcg ggttcactcc gcggacccag gcaacgccgt gctcacggta
     2221 ggtggggaag gcatagtcct ggtgacgcag cg
//
LOCUS       DATAIX010000215         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_11597, whole genome shotgun sequence.
ACCESSION   DATAIX010000215 DATAIX010000000
VERSION     DATAIX010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11597"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VHM65_03230"
     CDS             complement(<1..871)
                     /locus_tag="VHM65_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2704745.1"
                     /translation="MSKHLVLGAGPVGRHTAAELRRRGEEVVVGSRSGRGAGPGVAMV
                     ALDATDADAISRAADGVASIHNCLNPPQYHRWPQDWPPMAQALLVAAERCGAVLVTAS
                     NLYAYGPVDGPMREGMPDAATHSKGMVRARMWAEALAAHTAGRVHAVEVRASDYVGSG
                     VGAGSHLNRLVPGMLQGRTARVIGDADAAHTWTDVGDVGRTMAHLALTPASWGRVWHA
                     PSNPPRTQREALTDICAAAGRPAPKVAVIPRSVLRALGLVVPIVRELNGVAYQFDRPF
                     VMDSSAAERDLGLA"
     gene            988..1689
                     /locus_tag="VHM65_03235"
     CDS             988..1689
                     /locus_tag="VHM65_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2704746.1"
                     /translation="MPTSAPRLARALAREHLTAQIVAAARRQLTEVGPSELSVRAVAR
                     ELAMSSSAVYRYVASRDELLTLLLVEVYNELGDVTEAAELAVSDRTDLHTRWMTLCRA
                     IRAWARGHPHDYALVYGSPVPGYAAPPDTVPPATRVVAAFLRLLADIPWSRHVAGSAP
                     VAPDLAHAVGSLRALTPSGTPDLVIVRCLVAWTAVFGTISFELFGHLVGAVDDVDAYA
                     DLAFGRLAADLLGLS"
BASE COUNT          213 a          651 c          589 g          259 t
ORIGIN      
        1 gagccagccc gagatcgcgc tcggccgccg aggagtccat gacgaacggc cggtcgaact
       61 ggtaggcgac cccattcagc tcacgcacga tcgggaccac gagcccgagg gcgcgaagca
      121 ccgaacgggg gatgaccgcg accttgggcg cgggacggcc ggccgcggcg cagatgtcgg
      181 tgagcgcctc gcgctgggtc cgaggggggt tgctcggcgc gtgccagacg cgcccccagg
      241 acgccggtgt gagcgcgagg tgggccatcg tgcggccgac atcgccgacg tctgtccacg
      301 tgtgggccgc gtcggcgtcc ccgatgaccc tggccgtcct gccctggagc atccccggca
      361 cgagccggtt gaggtggctg cccgcgccga cgcccgaccc gacgtagtcc gacgcccgga
      421 cctcgaccgc gtgcacccgc ccggccgtat gggcagcgag cgcctcggcc cacatccgcg
      481 cccggaccat gcccttggag tgcgtcgcgg catctggcat gccctcgcgc atcggtccgt
      541 cgacagggcc gtaggcgtac aggttcgacg cggtgacgag aaccgccccg cagcgttcgg
      601 cggcgaccag gagcgcctgc gccatcggcg gccagtcctg cggccagcga tggtactgcg
      661 gtgggttgag gcagttgtgg atcgaggcga ccccgtcggc agcgcgggag atcgcgtcgg
      721 cgtcggtggc gtcgagcgcc accatcgcaa cgcccgggcc ggcgccgcgg cccgaccggc
      781 tcccgacgac gacctcctcg ccgcgtcggc gcagctcggc agccgtgtgc cggccgacgg
      841 gtccggcgcc aaggaccagg tgcttgctca tgtcactccc tttgcgatca atgctctcgt
      901 ttcagagcat tgcactcgcg ctctcgtttg gcaagagcgg tgctctcgtt tgttgtcacc
      961 gctctcgctc gatgggatag tgacggcatg ccgacatccg ccccccgcct ggcccgcgcc
     1021 ctcgcccgcg agcacctcac cgcgcagatc gtggccgctg cccggcgcca gctcaccgag
     1081 gtcgggccgt ccgagctgtc ggtccgcgcc gtcgcgcgcg agctcgccat gtcctcctcc
     1141 gccgtctacc ggtatgtcgc gagccgtgac gagctgctca ccctgctgct cgtcgaggtc
     1201 tacaacgagc tcggcgacgt gaccgaggcc gcggagctgg ccgtctcgga ccgcaccgac
     1261 ctgcacaccc gatggatgac cctgtgccgg gccatccgcg cctgggccag gggacacccc
     1321 cacgactacg ccctggtcta cggctcaccc gtgcccgggt atgccgcgcc ccccgacacg
     1381 gtgcctcctg cgacccgggt cgtggcggcc ttcctccggc tgctggcgga catcccctgg
     1441 tccaggcacg tcgccggctc ggcacccgtg gcgcctgacc tggctcacgc ggtcggcagc
     1501 ctgcgggctc tgaccccgag cgggacgccg gacctggtga tcgtgcgctg cctcgtggcg
     1561 tggacagcgg tcttcggcac catctcgttc gagctgttcg gccacctcgt cggcgctgtc
     1621 gacgacgtcg acgcctacgc ggacctcgcc ttcggccggc tcgccgccga cctgctcggg
     1681 ctctcgtgac cgatggtacc tgattgcatc cc
//
LOCUS       DATAIX010000216         1673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_71762, whole genome shotgun sequence.
ACCESSION   DATAIX010000216 DATAIX010000000
VERSION     DATAIX010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71762"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1405)
                     /gene="murD"
                     /locus_tag="VHM65_03240"
     CDS             complement(<1..1405)
                     /gene="murD"
                     /locus_tag="VHM65_03240"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX2704747.1"
                     /translation="MSGTDASYEPRPGLTHGDADWSGLAVTVVGLGVSGFAAADALAE
                     RGATVSALSPDVTPAIEDRARVLDILGVDVVLGREDEPRRLAAAIGADLVVTSPGVRP
                     SDPLMLAAAAAGVPVWGEVELAWRMRAREGAASWLTITGTNGKTTTTKMLASILTAAG
                     LRATSAGNVGTPLLEAVLHPDPYDVLAVELSSFQLHWSRSISPVASVCLNVAPDHIDW
                     HGSYEEYVRAKGRVYDNTHLACIYNVEDPVTEELVIEADVVEGCRAVGFTLGVPSVSM
                     VGVVDDVLADRAFVEARQHSAAELGTLADLRGDAASVAPHYVANALAAAALARAYGVG
                     PLAVRNGLRAFRPDPHRIAEVATVDGVRYVNDSKATNPHAAAAALAAYDSVVWVAGGL
                     LKGADIDALVERVAPKLHGVVLIGADRAQIAGALTRHAPGVPVIDVASTDTEVMDLVV
                     AHASALARPGDNEVHDLS"
     gene            complement(1402..>1673)
                     /locus_tag="VHM65_03245"
     CDS             complement(1402..>1673)
                     /locus_tag="VHM65_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704748.1"
                     /translation="SGLAITTRTELLLVILGGLFVIITSSVVLQVASFKSTGKRIFRM
                     APLQHHFELVGWAEVTIVIRFWIVCGVFVALGLGVFYAERVVGAT"
BASE COUNT          261 a          579 c          618 g          215 t
ORIGIN      
        1 cactgaggtc atggacctcg ttgtcgcccg ggcgggcgag cgcgctcgca tgggcgacaa
       61 cgaggtccat gacctcagtg tccgtgctcg cgacatcgat gacggggacc ccgggcgcgt
      121 gtcgggtcag cgcgcctgcg atctgcgccc ggtccgcgcc gatgagcacg acaccgtgca
      181 gcttgggagc gacccgctcg acgagggcgt cgatatccgc gcccttgagc agccctccgg
      241 cgacccagac caccgagtcg taggccgcga gcgcggccgc ggcggcgtgc gggttggtcg
      301 ccttggagtc gttgacatag cggacgccgt cgaccgtcgc gacctcggcg atgcggtgag
      361 ggtccggccg gaaggcgcgc aggccgttgc gcacggcgag cggcccgacg ccatacgccc
      421 gggcgagcgc cgcggccgcg agcgcattgg cgacgtagtg gggtgccacg ctcgccgcgt
      481 cacccctgag atccgcgagg gtgcccagct cggccgccga gtgctggcgc gcctcgacga
      541 aggcccggtc ggcgagcacg tcgtcgacga cacccaccat cgagaccgag ggcacgccga
      601 gggtgaaacc gaccgcgcgg cagccctcga cgacatcggc ctcgatgacg agctcctccg
      661 tgaccgggtc ctcgacgttg tagatgcagg cgaggtgggt gttgtcgtag acccgtccct
      721 tggctcggac gtactcctca taggagccgt gccagtcgat gtggtcgggg gcgacgttga
      781 ggcagaccga cgcgacgggg gagatcgagc gcgaccagtg cagctggaag ctcgacagct
      841 cgacggcgag cacgtcatac gggtccgggt gcaggaccgc ctcgagaagt ggcgtcccga
      901 cgttgcccgc gctcgtcgcg cgcagccccg cggcggtgag gatcgaggca agcatcttgg
      961 tcgtcgtcgt cttgccgttg gtcccggtga tcgtcagcca cgaggcggcg ccctcgcgcg
     1021 cccgcatccg ccaggcgagc tcgacctccc cccacacggg gacccccgcg gcggccgcgg
     1081 cgagcatgag cgggtcgctc gggcgcacgc ccggcgaggt gacgacgaga tcggccccga
     1141 tggcggcggc gaggcggcgc ggctcgtcct cccggccgag cacgacgtcg acgccgagga
     1201 tgtcgaggac ccgggcgcgg tcctcgatcg ccggcgtgac gtcgggggag agcgcgctca
     1261 cggtggcacc gcgctcggcg agcgcgtcgg cggccgcgaa gccactgacg ccgaggccca
     1321 ccacggtgac ggcgagtccg gaccagtcgg catcgccgtg ggtcagaccc gggcgcggct
     1381 cgtatgacgc gtcggtgccg ctcatgtcgc acccaccacc cgctccgcgt agaagacccc
     1441 gagcccgagg gcaacgaaca caccgcagac gatccagaac cggatgacga tcgtcacctc
     1501 ggcccagccg acgagctcga agtggtgctg caggggtgcc atccggaaga tccgtttgcc
     1561 ggtcgacttg aaggaggcca cctgcaggac caccgaggac gtgatgatga cgaagagccc
     1621 cccgaggatg acgagcagca gctcggtcct ggtcgtgatg gccagacctg aga
//
LOCUS       DATAIX010000217         3982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_273414, whole genome shotgun sequence.
ACCESSION   DATAIX010000217 DATAIX010000000
VERSION     DATAIX010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273414"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VHM65_03250"
     CDS             complement(<1..197)
                     /locus_tag="VHM65_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704749.1"
                     /translation="MAAFTITREIDAPLRYVWAAATDWGGYARWFPMTRMRLDPLPVR
                     PGWGFAGLTGVGRLALRDSMV"
     gene            complement(200..574)
                     /locus_tag="VHM65_03255"
     CDS             complement(200..574)
                     /locus_tag="VHM65_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX2704750.1"
                     /translation="MVWVMLVVAVVVAALTVAAVAGRVDGSMGEPTSTLSHVPLPPEQ
                     VTESALGALRFDTGLRGYRMSQVDDVIDRLRRELADRDDQLAVLRAVVTPPDTSPPEP
                     AQPPAVAGPAASLRASMPIEAD"
     gene            complement(597..1361)
                     /locus_tag="VHM65_03260"
     CDS             complement(597..1361)
                     /locus_tag="VHM65_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX2704751.1"
                     /translation="MTDHVGTGRERHTGPVTLRRNRVPQTTTDQRLLDSPGSTEWLHA
                     DPWRVMRIQSEFVEGFGALAELGPAVSVFGSARTRPDDPIYDTGERLGRALAEAGYAV
                     ITGGGPGTMEAANKGAFEAGGTSVGLGIELPFEQGMNAYVDLGVNFRYFFARKTMFVK
                     YARGFIVLPGGFGTLDELFESLVLVQTGKVTSFPIVLMGVDYWSGLVDWLRTSCAASG
                     TIELADVDLLDVTDDVEQAVGFIVDHAYSGRPIDPS"
     gene            complement(1358..2437)
                     /gene="dapE"
                     /locus_tag="VHM65_03265"
     CDS             complement(1358..2437)
                     /gene="dapE"
                     /locus_tag="VHM65_03265"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEX2704752.1"
                     /translation="MTALDLSLDPVALTAAVCDIESVSGDERALADAVEAALRGVPHL
                     DVIRDGDTIVARTSSGRPERVILAGHLDTVPLTTTPNLPTRRETGADGTELLWGRGSV
                     DMKGGIAVALRLAQQLSAPTREVTYVFYECEEVESERNGLARLAATRPDLLEADFAVL
                     LEPTDGGIEGGCKGTLSVEVTATGIAAHSARPWKGHNAIHEAGTVLNRLVAYEPRTVN
                     VEGLDFHEALSAVAISGGIAGNVIPDRCSVSVNYRFAPDRSGVEALAHVREVFAGYDV
                     TLTDTADGARPGLHLAAAQSFVEALGLPVAAKEGWTDVARFSTLGIPAVNFGPGDPSL
                     AHADDERCPVHQITGAEAALLRWLS"
     gene            2517..3479
                     /gene="dapD"
                     /locus_tag="VHM65_03270"
     CDS             2517..3479
                     /gene="dapD"
                     /locus_tag="VHM65_03270"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015583082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX2704753.1"
                     /translation="MRTAPVSEESTPGRRLAWGHGLATLTDGGQVLDTWYPNPRLGRR
                     PADSTVPDELAPLAAVDVARRTRTEVVTTEIDLDAPPASTPDAYLRLHLLSHRLAAPN
                     MVNLDGIFAALPNVVWTSQGPCAVEGFELTRARLRARGPVTVYGVDKFPRMVDYIVPS
                     GVRIADGDRVRLGAHLASGTTVMHEGFVNFNAGTLGTSMVEGRISQGVVVGDGSDIGG
                     GASTMGTLSGGGTERVRIGERCLLGAESGLGIALGDDCVIEAGLYVTAGTKVTLPDGT
                     VVKARELSGQDGLLFLRDSVTGAVVVRARDGHGITLNDTLHAND"
     gene            3466..3972
                     /locus_tag="VHM65_03275"
     CDS             3466..3972
                     /locus_tag="VHM65_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704754.1"
                     /translation="MRTTERSFTLAPGPRRRRRWPGRVLLLALLAVAVGGGWLGAKTL
                     FATVLPPVCQATASGTTFSLEPDQAANAALIAGVAQRRKLAPRAVTIALATALQESKL
                     RNLGHGDRDSLGLFQQRPSQGWGTATQILDPVYSTNKFFDALVKVKGYETMEITVVAQ
                     QVQRSGFP"
BASE COUNT          575 a         1448 c         1409 g          550 t
ORIGIN      
        1 atgaccatgg agtcgcgcag agccagccgc ccgaccccgg tcaggcccgc gaacccccag
       61 cccggacgga cgggcagcgg gtccagccgc atccgcgtca tggggaacca gcgggcatag
      121 ccgccccagt cagttgccgc agcccagaca taccgcagcg gcgcatcgat ctcgcgggtg
      181 atcgtgaagg ccgccatggt cagtccgcct cgatcggcat ggacgcccgg agggaggcgg
      241 ccggcccagc caccgcgggg ggctgggccg gctcgggagg cgaggtgtca ggaggggtca
      301 cgacggcgcg caggacggcg agctggtcgt cgcggtccgc cagctcgcgg cgcagccggt
      361 cgatgacgtc gtcgacctgg ctcatccggt agccccgcag cccggtgtcg aagcgcagcg
      421 cccccagcgc ggactcggtg acttgctccg gcgggagcgg cacgtggctc agcgtggagg
      481 tcggctcccc catcgacccg tcgactcgtc cggcgacggc agccaccgtc agggccgcga
      541 ccacgaccgc caccacgagc atcacccaga ccatcggacg atcgtgccat gccgggctag
      601 gacgggtcga tcgggcgtcc gctgtacgcg tggtcgacga tgaagccgac ggcctgctcg
      661 acgtcgtcgg tgacgtcgag caggtcgacg tcggccagct cgatcgtccc ggacgcggcg
      721 cacgaggtgc gcagccagtc gacgagcccg ctccagtagt cgacccccat gagcacgatc
      781 gggaacgagg tcaccttgcc cgtctggacg aggaccaggg actcgaacag ctcgtcgagg
      841 gtgccgaagc cgccgggcag gacgatgaac ccgcgggcgt acttgacgaa catcgtcttg
      901 cgggcgaaga agtagcgaaa gttgaccccg aggtcgacgt acgcgttcat cccctgctcg
      961 aagggcagct cgatgccgag cccgaccgac gtcccgccgg cctcgaaggc acccttgttg
     1021 gcggcctcca tcgtacccgg cccaccgccg gtgatcacgg catacccggc ctcggcgagc
     1081 gctcggccga gccgctcgcc ggtgtcgtag atggggtcgt cgggccgggt ccgggccgac
     1141 ccgaagacac tcaccgccgg accgagctca gccagggcgc cgaacccttc gacgaactcg
     1201 ctctggatcc gcatcacccg ccacgggtcg gcgtgcagcc actcggtcga tcccgggctg
     1261 tcgagcaggc gctggtcggt cgtcgtctgg gggacccggt tgcgccgcag ggtgaccggg
     1321 cccgtatgcc gctcgcgccc ggtccccacg tggtcggtca tgacagccac ctgagcaggg
     1381 ccgcctcggc gccggtgatc tggtgcaccg ggcagcgttc gtcgtcggcg tgggccaggc
     1441 tcgggtcacc cggcccgaag ttgacggcgg ggatgcccag ggtggagaac cgcgcgacat
     1501 cggtccagcc ctccttggcc gcgacgggca gcccgagcgc ctcgacgaag gactgtgccg
     1561 ccgccagatg caggccgggg cgggctccgt ccgcggtgtc ggtcagcgtg acgtcatacc
     1621 ccgcgaacac ctcccgcacg tgcgcgagcg cctccacgcc gctgcggtcc ggcgcgaagc
     1681 ggtagttgac cgagaccgag catcggtcgg gtatgacgtt gcccgcgatg ccgccgctga
     1741 tggcaaccgc gctgagcgcc tcgtggaagt ccaggccctc gacgttgacc gtgcggggct
     1801 cgtaggcgac gaggcggttc aggaccgtcc cggcctcgtg gatggcgttg tggcccttcc
     1861 acgggcgagc cgagtgggcg gcgatgcccg tggccgtgac ctcgacgctc agggtgccct
     1921 tgcagccgcc ctcgatcccc ccgtcggtgg gctcgagcag gaccgcgaag tcggcctcga
     1981 gcaggtcagg gcgggtggct gcgaggcggg ccaggccgtt gcgctcggac tcgacctcct
     2041 cgcattcgta gaagacgtag gtgacctccc gggtcggggc gctcagctgc tgggcgagcc
     2101 gcagcgccac ggcgataccg cccttcatgt cgacgctccc gcgcccccag agcagctcag
     2161 tgccgtcggc gccggtctcg cgccgggtcg gcaggttcgg agtcgtcgtc agtggcacgg
     2221 tgtcaagatg cccggcgagg atgacgcgct cgggtcgccc ggatgacgtc cgggcgacga
     2281 tcgtgtcacc gtcgcggatc acgtcgagat ggggcacccc cctcagggcc gcctcgacgg
     2341 catcggccag ggcccgctcg tcaccgctga ccgactcgat gtcgcagacg gcggcggtga
     2401 gagcgacggg gtcgagggac aggtccagtg cggtcatgag gcacaaggct atgacgtcca
     2461 cgcggaaggg gggcgcaatc cggacggcgt cggcatcgct cattaggctg gtcgccatga
     2521 gaaccgcccc cgtcagcgag gagagcaccc ccggccgccg gctcgcctgg ggccacggac
     2581 tggccacgct gaccgacggt gggcaggtgc tcgacacctg gtatcccaac ccccggctcg
     2641 gccggcgccc ggccgactcg acggtccccg acgagctcgc ccccctcgcc gcggtcgacg
     2701 tggcgcgccg cacgcggacc gaggtcgtca ccaccgagat cgacctcgac gcgccccctg
     2761 cgagcacgcc cgatgcctac ctgcgcctgc atctgctctc gcaccggctc gccgcgccca
     2821 acatggtcaa cctcgacggg atcttcgccg ccctgcccaa tgtcgtctgg acgagccagg
     2881 gcccgtgtgc cgtcgagggg ttcgagctca cccgggcccg gcttcgagcc cgcggaccgg
     2941 tcaccgtcta cggtgtcgac aagttcccgc ggatggtcga ctacatcgtc ccgtcggggg
     3001 tgcgcatcgc cgacggcgac cgggtgcgac tcggtgccca tcttgcgagt ggcacgaccg
     3061 tcatgcacga gggcttcgtc aacttcaacg ccggcacgct cggcacctcg atggtggagg
     3121 gccgcatcag ccagggcgtc gtcgtcgggg acggctcgga catcggcggc ggcgcctcga
     3181 cgatgggcac cctgtccggc ggtggcaccg agcgcgtccg catcggcgag cgctgcctgc
     3241 tcggtgccga gtccggcctc gggatcgccc tcggggacga ctgcgtcatc gaggccgggc
     3301 tctatgtcac ggccggcacc aaggtgaccc tgccggacgg caccgtcgtc aaggctcgcg
     3361 agctctccgg tcaggacggc ctgctcttcc tgcgcgactc ggtcaccggc gcggtcgtgg
     3421 tcagggcacg ggacggccat ggcatcaccc tcaacgacac gctgcatgcg aacgactgag
     3481 cgctccttca ccctcgcgcc cgggccgcgc cgacgtcgcc gctggccggg gcgtgtcctg
     3541 ctgctcgcgc tcctcgccgt cgccgtcggc gggggctggc tgggtgccaa gaccctcttc
     3601 gccaccgtgc tcccaccggt gtgccaggcc acggcctcgg gcacgacctt cagcctggag
     3661 ccggaccagg ccgccaacgc ggcgcttatc gcgggggttg cccagcggcg caagctggca
     3721 ccccgggcgg tgacgatcgc cctcgccacg gccctgcagg agtccaagct gcgcaacctc
     3781 ggccacggcg accgcgactc cctcggcctg ttccagcagc ggccgagcca gggatggggg
     3841 acggcgacgc agatcctcga cccggtctac tcgacgaaca aattcttcga cgccctcgtc
     3901 aaggtcaagg gctacgagac gatggagatc accgtcgtcg cccagcaggt ccagcgcagc
     3961 ggcttcccct aggcgtatgc cg
//
LOCUS       DATAIX010000218         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_41788, whole genome shotgun sequence.
ACCESSION   DATAIX010000218 DATAIX010000000
VERSION     DATAIX010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41788"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VHM65_03280"
     CDS             complement(<1..236)
                     /locus_tag="VHM65_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704755.1"
                     /translation="MTTPPSPAPASGASGLREGLAVIAVGVRAQPRPFVVSVLGSALY
                     GVMTVLTAWAVGAVVRDVVTPAVAARTVTAGQLW"
     gene            277..927
                     /locus_tag="VHM65_03285"
     CDS             277..927
                     /locus_tag="VHM65_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03085 family metal-binding protein"
                     /protein_id="HEX2704756.1"
                     /translation="MMAPVTHLARAERYALCETFLAVGPDAPTLCSPWRSRDLAAHLV
                     LRESRPDLTAGIVIPALAGRLERTQHDRAMKDWPDLVRDFRQGPPMWWPARLRILDDA
                     ANLSEFYIHHEDVLRAADEPMSRELDPGLEAALWSSIKRHGPLAVRRSPVGVVLVAEG
                     HGRAAVMRPGTGGTVVLHGAPGELVLHLSGRQDHARVHITGEPADVSAFEQVKFGI"
     gene            complement(934..>1521)
                     /locus_tag="VHM65_03290"
     CDS             complement(934..>1521)
                     /locus_tag="VHM65_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX2704757.1"
                     /translation="TYEVIGRTAEQVFIPLTVGGGVRTVEDVDRLLRAGADKVGVNTA
                     AIERPELISEVADRFGAQVLVLSADVRRRLGHGGRATHDFVMTTHGGRTPVDLDAVQW
                     CARAAGLGAGEILLNSMDADGTKDGFDLELIAAVRAAVGIPIIASGGAGRAEHFAPAV
                     WAGADAVLAASVFHFGELTVGEVKAAMRAAGVTVR"
BASE COUNT          210 a          599 c          507 g          205 t
ORIGIN      
        1 tgccagagct ggccggccgt caccgtcctc gcggcgaccg ccggagtgac gacgtcacgg
       61 acgaccgcgc cgaccgccca cgcggtgagg accgtcatga cgccatacag ggcgctcccg
      121 agcacggaga cgacgaacgg ccgcggctgg gcgcgcaccc cgacggcgat gaccgccagt
      181 ccctcacgca ggccggacgc cccactggcg ggtgcggggc tcgggggagt ggtcacaaca
      241 accgagcctg tcacacacct ccgacagcat gctgcgatga tggcgcccgt gacacatctg
      301 gcccgcgccg agcgctacgc cctctgtgag accttccttg cggtcggccc cgacgcaccg
      361 acgctgtgca gcccctggcg ctcgcgcgac ctcgctgcgc acctcgtcct gcgcgagtct
      421 cgccccgacc tgaccgccgg gatcgtcatc cctgccctcg cgggccggct cgagcgcacc
      481 cagcacgacc gcgcgatgaa ggactggccc gacctcgtgc gcgacttccg ccagggtccc
      541 ccgatgtggt ggcccgcccg cctgcgcatc ctcgacgatg cggccaacct ctccgagttc
      601 tacatccacc acgaggacgt cctgcgcgcg gccgacgagc cgatgtcccg tgagctcgac
      661 cccggcctcg aggcggcgct ctggtcctcg atcaagcgcc acgggcccct cgccgtccgg
      721 cgcagcccgg tcggggtcgt gctcgtcgcc gagggccatg gccgcgccgc ggtgatgagg
      781 cccggcacgg gtggcaccgt cgtgctgcac ggcgccccgg gtgagctggt tctgcacctc
      841 tccggtcgcc aggaccacgc tcgcgtccac atcaccgggg agcccgcaga cgtcagcgcc
      901 ttcgagcagg tcaagttcgg gatctgagcc cggctagcgg acggtcaccc cggccgcccg
      961 catcgcggcc ttgacctcac ccacggtcag ctcgccgaag tggaagacgc tcgccgccag
     1021 gacggcgtcg gcgcccgccc ataccgccgg tgcgaagtgc tcagcgcgcc cggcgccccc
     1081 actggcgatg atcgggatgc ccaccgcagc tcgcacggcg gcgatgagct cgaggtcgaa
     1141 cccgtccttg gtcccgtcgg cgtccatcga gttgagcagg atctccccgg ctcccagccc
     1201 ggccgctcgc gcgcaccact gcacggcgtc gagatcgacc ggtgtgcggc cgccgtgcgt
     1261 cgtcatgacg aagtcgtggg tcgctcggcc accgtggccg agccgtcggc gcacgtcggc
     1321 cgaaaggacg agcacctgag ccccgaagcg gtcggccacc tcgctgatca gctcgggccg
     1381 ctcgatggct gcggtgttga cgccgacctt gtccgctccg gcgcgcagca gacggtcgac
     1441 gtcctcgacc gtccggaccc cgccgccgac cgtcagcggg atgaagacct gctccgcggt
     1501 ccggccgatg acctcgtatg t
//
LOCUS       DATAIX010000219         3614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_71907, whole genome shotgun sequence.
ACCESSION   DATAIX010000219 DATAIX010000000
VERSION     DATAIX010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71907"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VHM65_03295"
     CDS             complement(<1..365)
                     /locus_tag="VHM65_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704758.1"
                     /translation="MASSASRAESLAQKLLDAQVGYHLDRLTGDQLVTTVSRLADDLL
                     AAADQHQLVDLVDRAAIKAIVVRALRSVPGSSAVSGIVELATTVVYDGPAESYPLGEP
                     VEREQIEVLLDELLALNPLL"
     gene            complement(1016..3529)
                     /locus_tag="VHM65_03300"
     CDS             complement(1016..3529)
                     /locus_tag="VHM65_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2704759.1"
                     /translation="MTTRTALRLWPGSRRLAGVLLGVALPLLLLGVAQPVSAARSAGV
                     VRTDPATDRQGPLVIVLDLSGSMNDQDANGVVKLEGAKSALEKLVGRQPVGAELGLWT
                     YPGGQVDPTSGCSPGEFEIGGVIAEVTDPADLAAQIDILTADGGTPTGPALRTVADRL
                     KALGYTGATILLVSDGESNCGPPPCEVAQQIVDEGFDVTVDALGFRLSEAGTQEMTCI
                     SSTTGGRYVPVDDTAQLDEYLTTLAVPNLALQVTAPTQAIVGQGITVSATVSNPSSRP
                     VADAQISLTFNDKTSQAIFPAVLPPRYRLGNIAPGGSVSRTWTITAGVDLTGGAASYR
                     VSTWGKGVGATDKSGTITVNSTEGLKNAAGPWLKSINDTGNVLIMGDSYSAGEGAPPY
                     VDGTDKGTNRCHRSDKTHTYPQFDTGRRTNIACSGAVVNNMESAQYVGDAEENRPQFA
                     QLRDMDVKPDLVVLTLGGNDIGFGDIIKGCLGPSNCSESDLRTKTAVKIAALNQPQDT
                     TKPGSLTQAYLDIYETLNAHDAEAKRKRVAPLLVLAYPLVLPVTNTTKCAGFSQTEVV
                     FAKGVIASLNTTIERAVRLAAEGGRHIYFVGDTSRAVQPNHTACDPDPYIRDVNALSG
                     LAAKAFDEWLKAQAAKFRTNDYPSLTQQFMHPLEKGYQAMTVATYIWSTSLDLDLGPG
                     RPIPPAYDYVEPEGVDLWLAGATVRPGETETVTPGSTTTVEADGLPPHHPVLISLNSV
                     RRTLATSMTDEAGRLIQVVTIPADTPIGAHTLHIEYLQEGAEKSLDATLHLHAPIPLW
                     VLAIATFGLLGAVGALALGLREHRSRRQTSQRLVAPSTI"
BASE COUNT          545 a         1169 c         1296 g          604 t
ORIGIN      
        1 agcagggggt tcaaggcgag cagctcgtcg aggagcacct caatctgctc gcgctcaacg
       61 ggctccccga gcgggtagga ctcggcgggt ccgtcgtaca caaccgtggt cgcgagctcg
      121 acgatgccgc tgacggcgga gcttcccggc acgctcctga gagcccgcac cacgatggcc
      181 ttgatcgccg cccggtcgac gaggtcgacg agctgatgct ggtccgccgc cgccagcagg
      241 tcgtcggcga gacgggacac cgtggtcacg agctgatctc cggtcagccg gtcaaggtgg
      301 tagccgacct gcgcgtcgag cagcttctgg gcaagggact ctgctcgcga agcgctactg
      361 gccatgggga aacggtacgg gacggcgcgg aggaccgtag cgctcccagc cactcacgcg
      421 tgatctctgc tgccgctcat cggctacgtg agggcgaaat gaccatcgtg ccggaccctt
      481 cagtgccgag gacgccagca gcgttgggcc cgcgggaacg ccaccatcga cgtccatatc
      541 cctgaaggtg ccggcgaacg tctgcccttg cggaccggcg ccacgggaag tccgaccatc
      601 cagaccgggc ggccttcagg cccgggagcg ggtcgtgacc cgcgaggagg tcttcgagct
      661 gagcaacccg tccgggccgg tcagatcctc accccgcacc cgagcagcct ctaccagttg
      721 acgcaccgcg gcacgctcag cctccgccgc cgggaccgtc ttccctactg ccatctcaag
      781 cggactcatg tcccccagtg tctcggtcat cctcgaccat ccttcctgcc gaacctggct
      841 caacgccgct tgacaacgca taggagcatc agtctcgcgt ggtgtagagg caggacggcg
      901 gaccgacgta tgccggtcgg cgggtgaagc tggagtagcc gccgggcgct tcgggatgac
      961 tccggtcgtg tcggagcgcc gctcgggctg tatcggggcg cggttcttgc ccctgtcaga
     1021 tggtcgaggg ggcgacaagc cgctgggacg tttggcggcg cgaacggtgt tcccgtagcc
     1081 cgagcgcgag cgctccgacg gccccgagca ggccgaaggt ggcgatggca aggacccaca
     1141 ggggaatcgg tgcatgcaga tgcagcgtcg cgtcgagtga cttctcggcc ccttcctgca
     1201 ggtactcgat gtggagggta tgcgcgccga tcggggtgtc ggcagggatg gtcaccacct
     1261 ggatgagccg accggcctcg tcggtcatcg aggtggccag cgtgcgtcgc acgctgttga
     1321 ggctgatgag gaccgggtga tggggtggca ggccgtcggc ctcgacggtg gtggtcgacc
     1381 caggagtcac cgtctccgtc tccccgggcc gcacagtcgc gccggcgagc cagaggtcca
     1441 cgccctcggg ctcgacgtag tcgtaggcgg gtgggatcgg ccgacccgga ccgaggtcca
     1501 ggtcgagtga ggtcgaccag atgtatgtcg cgacggtcat cgcctggtag cccttctcca
     1561 ggggatgcat gaactgctgg gtcagtgagg ggtagtcgtt ggtccggaac ttggctgcct
     1621 gagctttgag ccactcgtcg aaggccttcg cggccagtcc tgacagggcg ttgacgtcac
     1681 ggatgtaagg gtccgggtcg cacgccgtgt ggttcggctg gacggcccgg gatgtgtcgc
     1741 cgacgaagta gatgtggcgg ccgccctccg ccgcaagtct taccgcccgc tcgatcgtcg
     1801 tgttgaggct ggcgatgacg cccttggcga agacaacctc agtctgcgag aagcccgcgc
     1861 acttcgtggt gttggtgacg gggagcacca gcgggtacgc cagcacgagg agcggagcga
     1921 cccgcttacg cttcgcctca gcgtcgtgcg cgttcagggt ttcgtagatg tcgaggtacg
     1981 cctgggtgag cgaaccgggc ttcgtggtgt cctggggctg gttgagggcc gcgatcttca
     2041 cagcggtctt ggttcggagg tcggactcgc tgcagttcga cgggccgagg cagcccttga
     2101 tgatgtcccc gaagccgatg tcgttgccgc cgagcgtcag aaccacgaga tcgggtttga
     2161 catccatgtc gcgcagctgc gcaaactggg gccggttctc ctcggcgtct ccgacatact
     2221 gcgccgactc catgttattg acgaccgccc ccgagcaggc gatgttcgtc cggcgcccgg
     2281 tgtcgaactg cgggtaggtg tgcgtcttgt ccgaccggtg gcagcggttc gtgcccttgt
     2341 ccgtgccgtc gacgtagggc ggggctccct cccccgcgga gtaggagtcc cccatgatga
     2401 ggacgttccc cgtgtcgttg atgctcttga gccaagggcc ggcggcgttc ttcagcccct
     2461 cggtggagtt gaccgtgatc gtccccgatt tgtcggtcgc gcccacccct ttcccccagg
     2521 tgctcacccg gtatgacgca gcaccgccgg tgaggtccac cccggcggtg atggtccagg
     2581 tgcgtgacac cgacccccca ggcgcgatgt tgcccaggcg atagcgaggt ggaaggacgg
     2641 ccgggaagat ggcctgcgat gtcttgtcgt tgaaggtcag ggagatctgg gcgtcggcca
     2701 cgggccggga cgaggggttg gagaccgtgg cggacaccgt gatcccctga ccgacgatgg
     2761 cctgcgtcgg cgccgtaacc tgcagtgcga ggttaggcac ggccaaggtc gtgaggtact
     2821 cgtcgagctg tgcggtgtcg tcgacgggca cgtagcggcc gccggtcgtc gacgagatgc
     2881 aggtcatctc ctgggtgccg gcttcggaga gccggaaccc cagggcatcg acggtgacgt
     2941 cgaagccttc gtcgacgatc tgttgcgcca cctcgcacgg cggcgggccg cagttggact
     3001 ccccgtcgga cacgagcagg atcgtcgccc cggtgtagcc gagggccttg agccgatccg
     3061 cgaccgtgcg gagagccgga ccggtggggg tgccgccgtc ggcggtgagg atgtcgatct
     3121 gtgccgcgag gtcggccggg tcagtcacct cggcgatcac gccgccgatc tcgaactcac
     3181 cgggagagca cccggacgtc gggtccacct ggcctccggg gtaggtccac agcccgagct
     3241 cggcgccgac cggctggcgc cctacgagct tctcgagagc ggacttcgcg ccctcgagct
     3301 tcaccacacc gttggcgtcc tggtcgttca tcgagcccga caggtccagg acgatgacca
     3361 gcggaccttg gcggtctgtc gccggatcgg tgcggaccac ccccgcggat cgagccgccg
     3421 acacgggttg ggctacgccg agcaggagca ggggtagagc gacgccaagg agcaccccgg
     3481 ccaggcgcct tgagcccggc cacagccgga gcgcggtccg tgtggtcacg gccgcgcccc
     3541 gatccgcgtg ccgagcagcg gcccgccgac gagccgagcg tccccgagga cggcgtcggt
     3601 ggacgcgtcg ggat
//
LOCUS       DATAIX010000220         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_11832, whole genome shotgun sequence.
ACCESSION   DATAIX010000220 DATAIX010000000
VERSION     DATAIX010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11832"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..908)
                     /locus_tag="VHM65_03305"
     CDS             complement(186..908)
                     /locus_tag="VHM65_03305"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX2704760.1"
                     /translation="MGFPENVLAKDEKVVRSLHPHVLTVVGPAFVGLVLLAVAVAVAR
                     VTPDDAKGNWIQWISVALIVLVAVPLVLVPFLRWRTTRYVVTTHRVMLRRGILSKAGK
                     DITLSKITDVSFAQTLLDRMIRSGTLLIESAGDSPNEMFRNIPHSNDVQQLINGLIDE
                     DANRRSAVIQERFLRESDVFDQTRDERRAPVLPPPAQSPSAPTAPSPSAPTAEETQVV
                     SAPPPRSQPPLPPSRPTAGPAE"
     gene            1227..1802
                     /gene="thpR"
                     /locus_tag="VHM65_03310"
     CDS             1227..1802
                     /gene="thpR"
                     /locus_tag="VHM65_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX2704761.1"
                     /translation="MPHRLFVAVRLPDEVSGDLEAFLEPRRDAGTELRWTRPEHWHVT
                     LAFMEAVSDRAVEPLVERLTAAAARCPRFPLWLGGAGAFPHPYDARVLWLGVRGPEQD
                     ESPALARLARSARAAANAAGAAPHGAAFRAHVSLARSRRPVEATRWLRTLGTYVSAGF
                     VVDRIELVASHLGEGPRRTPRHETLDTFALS"
BASE COUNT          302 a          739 c          743 g          312 t
ORIGIN      
        1 ctcggcgatc gcgagaagcc ggcacacgtc gtacatcgac tcccctgcca tcccgaccgc
       61 ggcccgggat cgactcttcc gggccggcgc cgaggttgag gtcgcgcatg aacgagcgca
      121 tcgcagccag ccgccgcagg acgtcctcga ccgtcccgat cgaccccgcc gcgagatagc
      181 tggcgctact ccgccgggcc cgccgtggga cgtgacggtg ggagcggtgg ctgagatcgc
      241 ggcggcggcg ccgagaccac ctgcgtctcc tcggccgtcg gcgccgaggg cgagggcgcc
      301 gtcggcgccg agggcgactg ggcgggcgga ggcagcacgg gcgcccggcg ctcgtcacga
      361 gtctggtcga agacgtccga ctcgcgcagg aagcgctcct ggatcaccgc gcttcgccgg
      421 ttggcgtcct cgtcgatgag gccgttgatc agctgctgga cgtcgttgct gtgggggata
      481 ttgcggaaca tctcgttggg gctgtcgccg gccgactcga tgagcagcgt gccggagcgg
      541 atcatccggt cgagcagggt ctgggcgaag ctcacgtcgg tgatcttgga cagcgtgatg
      601 tccttgccgg ccttgctcag gattccccgg cggagcatca cccgatgggt cgtgacgacg
      661 tagcgggtcg tgcgccagcg caggaacggc acgaggacga gcggcaccgc cacgaggacg
      721 atgagcgcca ccgagatcca ctggatccag ttgcccttgg catcgtcggg ggtgaccctg
      781 gccaccgcaa cagcgacggc cagcagaacg agcccgacga acgccggacc gaccacggtg
      841 aggacgtgtg gatgcaggct gcggacgacc ttctcgtcct tggccagcac attctcggga
      901 aagcccactg cgaacctcct gcgcgagcga cgggcctgca ccgatccgca cacccagggc
      961 caggctatcg gcaccacggt catggtgccg ggtccgacgc gacgtggtat gacgcccccc
     1021 ggcagatggg ctccggcccg gggaccgctg aagaaggggc caccggcccc atcctcgcga
     1081 cggttactgg cgcctcgcgt ggcaaatctg cagcgcgagg agccacttta cggcgcgtgg
     1141 atgaggacgg ggggccatct cggcgcgggg cggccgggcc gaaacccggg tgccgcccgg
     1201 tcgcccgtga cgtagcctcg catccgatgc cgcaccgtct gttcgtcgcc gtccgactgc
     1261 ccgacgaggt cagcggggat ctcgaggcgt tcctcgagcc ccgccgcgac gccgggacag
     1321 agctgcgctg gacccggccc gagcactggc acgtcaccct cgccttcatg gaggcggtca
     1381 gtgaccgggc ggtcgagccc ctggtcgagc gcctgaccgc cgcggccgca cggtgcccac
     1441 ggtttccgct gtggctcggg ggggccggag ccttccctca cccgtatgac gcgcgcgtcc
     1501 tctggctggg cgtgcgcggt cccgagcagg acgagtcgcc cgccctcgct cggctggccc
     1561 ggagcgctcg ggcggcagcc aatgccgcgg gcgctgcgcc ccacggggcg gccttccggg
     1621 cccacgtgtc gctggctcgg agccgtcgtc ccgtcgaggc gacccgctgg ctgcgcacgc
     1681 tggggaccta cgtgagtgct gggttcgtgg tggaccggat cgagctcgtc gcgtcccacc
     1741 tcggtgaggg accgcgccgt acccctcggc acgagacgct cgacaccttc gcgctgtcgt
     1801 gagcgcgccc gacgtcggcc tgagcgagcc ccgacgacac cgaagctctg cctgcccgaa
     1861 ccgctctgcc tgcccgagtc ccgttgaccc gcacgtcggt ttgttctatc ttggggcgtt
     1921 tgtggcgaga actcaccact tcggacacgt tcggaaggag tccgtgtccg aacctcttta
     1981 catcgccccg agtctgtggc ataaatgtag tacgccccga caggcactga cccgaggacc
     2041 acgacgagcg aagggagccc acgtgcagga tccgctgctg tcgctctgcc gcgtac
//
LOCUS       DATAIX010000221         2646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_161366, whole genome shotgun sequence.
ACCESSION   DATAIX010000221 DATAIX010000000
VERSION     DATAIX010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161366"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="VHM65_03315"
     CDS             complement(<1..1274)
                     /locus_tag="VHM65_03315"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX2704762.1"
                     /translation="MTPTGKRHISPVDTIWLNMDRPNNLMVIISLMFLESVPAWDDLA
                     EVIRGGIVERFPVFSQRPTPPLVPVGLPSWEDDPDFSLEHHLHHVTLAAPGDDATLQR
                     YVDERIPVPLDRAHALWEIHLIDGYGSGAVLFCRFHHALADGIALTRVLLSMTEDGRG
                     ARSPDADRGDEVTGTHDHSLVRDMISTTMALARGGLEQVSHLAHPSAVVDALGLGVRT
                     SEVVADLLLAHNPDNVFNGTPGVRKRVVWTRAFPLADLKRVSRLAQATLNDLLLSAVA
                     GALHTYQRDHGAEPVDLVTMVPVNVRPLDQPLPRELGNQFAILFFKFPSETPTAVGRL
                     AETKRRMDWLKASPEAVLTFGLIAAIGRTTRDLERYIVDFFANKAIGVTTNVAGPTSI
                     RTFAGVPMTSVLGWVPGSGKQTVGVCIFTYAGT"
     gene            complement(1422..2273)
                     /locus_tag="VHM65_03320"
     CDS             complement(1422..2273)
                     /locus_tag="VHM65_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2704763.1"
                     /translation="MNALSPQTATLRGHSLSYLDSGAGPAILFVHGILGSQSSWAHLI
                     DRLDDAHRVIVPDLFGHGASAKPMGDYSLGAHAGTLRDLLDWLGIESVTLVGHSLGGG
                     ISMKFLYLFPERVERLVLVGSGGLGREVNPVLRSATLPGAELVLPVIASLWVRERAES
                     VGRVLSRFGWRPGSDLTEIWQGFASLGDADSRRAFLATTRAVIDPGGQTVSAHDYLPE
                     VNDVPTLVVWGTGDRMIPAWHATKAHGAIPGCRVVLFEGAGHFPHLEQPDRFAEVLRD
                     FIAESGS"
BASE COUNT          427 a          875 c          934 g          410 t
ORIGIN      
        1 gtcccggcat acgtgaaaat gcacaccccc accgtctgct tgcccgaccc cggcacccat
       61 cccagcacgc tcgtcatggg cacgccggcg aaggtgcgga tcgacgtcgg cccggccacg
      121 ttggtcgtga cgccgatggc cttgttcgcg aagaagtcga cgatgtagcg ctcgagatcg
      181 cgggtggtgc ggcctatcgc ggcgatgagc ccgaaggtaa ggacggcctc cggtgatgcc
      241 ttgagccagt ccatccggcg tttggtctcg gccagccggc ccaccgctgt cggcgtctcg
      301 ctcgggaact tgaagaagag aatcgcgaac tggttgccga gctcgcgagg cagcggctgg
      361 tcgagcgggc gcacgttgac cggcaccatc gtcacgaggt cgaccggctc ggcgccgtgg
      421 tccctctggt aggtgtgcag tgcccccgcg actgcgctga ggagtaggtc gttgagtgtg
      481 gcctgcgcta gccggctcac ccgcttgagg tcggccagcg ggaacgcccg ggtccagacg
      541 acccgtttac gcacaccggg tgtcccgttg aacacgttgt cggggttgtg cgccagcagc
      601 aggtcggcca cgacctcgct cgtgcgcacc cccagtccca gtgcatcgac cacggcggac
      661 gggtgggcca ggtgagacac ctgctccagg cccccccgcg ccagggccat ggtggtcgag
      721 atcatgtccc tgaccaggga gtggtcgtgg gtgccggtga cctcatcgcc acggtcggca
      781 tccggactcc gtgcgccccg cccgtcctcg gtcatcgaca gcaggacccg cgtcagggcg
      841 atcccgtcag ccagggcgtg gtggaagcgg cagaacaaga ccgccccgga cccgtagccg
      901 tcgatgaggt gaatctccca gagcgcgtgg gcgcgatcga gcggcacggg gatgcgctcg
      961 tcgacgtacc gctgaagggt cgcgtcgtcg cccggagccg ccagggtgac atggtgcagg
     1021 tggtgctcca gggagaagtc ggggtcgtcc tcccaggagg gcagtcccac tgggacgaga
     1081 ggcggggtgg gccgctggct gaacaccggg aagcgctcga cgatcccgcc ccggatgacc
     1141 tccgcgagat cgtcccaggc gggcaccgac tccaggaaca tgagcgagat gatgaccatg
     1201 aggttgttcg gccggtccat gttcagccag atggtgtcca ctgggctgat gtggcgcttt
     1261 ccggtgggtg tcatgctcgg ctccagttcg gtggaagggc cctgcgatgg gtcaacgacc
     1321 agcgtgggct ggcgcctacc gcagtgctag tggccatagt cccatctcgg ccattccgcg
     1381 gcccggcacg agcaccccgg cgcgggaacg ccgaggtggg ctcaggaccc ggattctgcg
     1441 atgaagtcac gaagcacctc cgcgaaccga tctggttgct cgaggtgagg gaagtggccg
     1501 gctccctcga acagcacgac gcggcagccg ggaatcgccc cgtgcgcctt ggtggcatgc
     1561 caggcgggga tcatccggtc accagtgccc cagacgacga gggtcggaac gtcgttgacc
     1621 tcgggcaggt agtcgtgcgc gctcacggtc tgaccgccgg ggtcgatgac cgcgcgcgtc
     1681 gtggccagaa acgcgcgcct gctgtcggca tcacccaagg acgcgaagcc ttgccagatc
     1741 tcggtcaggt ccgaccccgg ccgccagccg aaccgggaca ggacccggcc cacggactct
     1801 gcacgctcgc gcacccacaa tgaggcgatg accggcagca ccagctctgc tcccggcagg
     1861 gtggcggaac ggagcaccgg gttgacctcc cgtcccagcc ctccgctgcc gacaagcacc
     1921 agtcgttcca cccgctccgg gaacaggtac aggaacttca tcgagatgcc accgccgagg
     1981 gagtggccga ccagggtgac gctctcgatg ccgagccagt cgagcagatc acgcagggtg
     2041 ccggcgtggg cgccgagcga gtagtcgccc atcggcttgg ccgatgcacc atggccgaag
     2101 aggtccggca cgatcacccg gtgggcgtcg tccagccggt cgatgaggtg ggcccagctg
     2161 ctttgggacc cgagaatccc gtggacaaag aggatcgcgg gtcccgcacc gctgtcgagg
     2221 tacgacaggg agtgtccgcg cagcgtcgcc gtctgcgggc tcaacgcgtt cacggcgcct
     2281 cctcggtccg agtacggggg ctcttggagc atagcccgcc cggtgagtgc cctcgcgttc
     2341 agtggcgacc ccggctgatg agcggccatt tcggcggaca tatcggcccg atttggccgc
     2401 ggcagcggcg tgccaccgtt cgccatccgg gggtggccgc ctgcccctgc tgcgggttca
     2461 gccctgtcag tgccatgatg gcatggcggg caatcggacc atcggatcga cggtgagccg
     2521 gccgatcgga cgactgacga ggaggtggca atggctgacc acggcgatac gcatcgatac
     2581 ggactgctgc agtctgcttt cgaggagttc ctgcagggga cccgctccgt ggccaacctc
     2641 gtgggg
//
LOCUS       DATAIX010000222         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_273547, whole genome shotgun sequence.
ACCESSION   DATAIX010000222 DATAIX010000000
VERSION     DATAIX010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273547"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VHM65_03325"
     CDS             complement(<1..529)
                     /locus_tag="VHM65_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX2704764.1"
                     /translation="MSPSVAPRPMQDGGPEMIQLLTPEGERVHDPAHAAYAQIAAGLS
                     EEDLRGFYRDLVLIRRVDAEATALQRQGELGIWASLLGQEAAQVGSGRALRPQDYAFP
                     TYREHGVAWCRGVNPLNLLGLFRGVNHGGWDPHEKNFHLYTIVIGAQTLHATGYAMGV
                     QRDGAVGTGDAERDTA"
     gene            758..>1587
                     /locus_tag="VHM65_03330"
     CDS             758..>1587
                     /locus_tag="VHM65_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX2704765.1"
                     /translation="MASSRRGFTLATFVPGVAVLRGYERPWLRGDLVAGVTVAAYLIP
                     QVMAYAEVAGLPPVVGLWAIMGTMAVYAVVGSSRQLSVGPESTTALMTATAIAPLAAS
                     DPARYAGLAAVLALMVGAMCLVGWAARLGFLAELLSRPVLVGYMAGVAVIMAAGQLGK
                     VTGITVEADSVFGQLAYVVTHLGEANVATGVLSASVLALLVVGRQRFPRLPVPLIVML
                     LAAAVVAVLDLPSRGIAVIGSVPGGVPLPSVPSFSRSDLAALLLPALGVTVVGYTDNI
                     L"
BASE COUNT          168 a          551 c          601 g          267 t
ORIGIN      
        1 cggcagtgtc ccgctcggcg tcgccggtgc cgacggcgcc gtcgcgctgc acgcccatgg
       61 catagcccgt ggcgtgcagg gtctgagcgc cgatgacgat cgtgtagagg tggaagttct
      121 tctcgtgcgg gtcccagccg ccatggttga ccccgcggaa gagcccgagc aggttgagcg
      181 ggttgacccc ccggcaccac gccacgccgt gctcgcggta ggtcgggaac gcgtagtcct
      241 gcgggcgcag ggcgcgcccg gacccaacct gggcggcctc ctggccgagc aggctggccc
      301 agatcccgag ctcgccctgg cgctgcaagg ccgtggcctc ggcatcgacc cggcggatga
      361 ggacgaggtc gcggtagaac ccccgcaggt cctcctcgct cagcccggcg gcgatctggg
      421 cgtatgccgc gtgcgccggg tcgtggacgc gctcgccttc cggggtgagc agctggatca
      481 tctcgggccc gccgtcctgc atggggcgtg gcgcgacgga tggactcacg ggcactcctt
      541 ctccctggcg cgaccgcgtc gtcgcgtcag gttcgggccg gcggttcggt cacggggtgt
      601 ccgcggctgg ccgagcggcg tcgtggctgt gcggcgccag ggtgtgtcaa cgctcacagc
      661 gagggcaggc cccaacgtac tccaccaggg ccggatctca catgccgcaa gacctgaggg
      721 cggcacatcg gaccggcctg gtcctatgct tcgccgcatg gcgtcctcac gacgcgggtt
      781 caccctggcc accttcgtcc ctggggtggc ggtcctgcgc ggctacgagc ggccctggct
      841 gcgcggcgac ctcgtcgcgg gggtgaccgt cgcggcatac ctcatcccgc aggtgatggc
      901 gtatgccgag gtcgcgggcc tgccaccggt ggtggggctg tgggcgatca tgggcacgat
      961 ggccgtctat gccgtcgtcg ggtcgagccg gcagctctcg gtcggtcccg agtcgacgac
     1021 ggcgctgatg acggccaccg cgatcgcccc cctcgcggcc tcggacccgg cccggtatgc
     1081 cgggctcgct gccgtcctcg cgctcatggt cggggcgatg tgcctcgtgg gctgggccgc
     1141 tcggctgggg ttcctcgccg agctgttgtc gcggccggtg ctggtggggt acatggcggg
     1201 ggtggccgtg atcatggccg ccggccagct cggcaaggtc acggggatca cggtcgaggc
     1261 cgactcggtg ttcggccagc tcgcctacgt cgtgacgcac ctgggcgagg cgaacgtggc
     1321 caccggggtg ctgtcggcct cggtgctcgc cctgctcgtc gtcgggcgtc agcgcttccc
     1381 caggctgccg gtgccgctca tcgtcatgct gctcgccgct gcggtcgtcg cggtgctgga
     1441 cctgcccagc cgtgggatcg cggtgatcgg gtcggtgccc ggcggggtgc cgctgccgag
     1501 cgtgccgtcg ttctcgcgca gcgatctggc cgcgctgctg ctgccggcgc ttggtgtcac
     1561 cgtggtgggc tacaccgaca acatcct
//
LOCUS       DATAIX010000223         5416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_42042, whole genome shotgun sequence.
ACCESSION   DATAIX010000223 DATAIX010000000
VERSION     DATAIX010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 5416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5416
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42042"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VHM65_03335"
     CDS             <1..441
                     /locus_tag="VHM65_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX2704766.1"
                     /translation="EALPLNGNGKLDLAALPRQRTLSAAPAAPAVAPADPLEQSIAQV
                     WQTVLGLASVGVDDNFFDAGGTSIRLVAVQSGLQALGRPVSVIDLFQYPTIRSLARHL
                     SDRDNRADIGQPSSDRRIAARRGLRRRPADRVPPSAPQSEPSAS"
     gene            515..>5416
                     /locus_tag="VHM65_03340"
     CDS             515..>5416
                     /locus_tag="VHM65_03340"
                     /EC_number="6.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0034081 - polyketide synthase complex
                     [Evidence IEA];
                     GO_process: GO:0030639 - polyketide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEX2704767.1"
                     /translation="MNDDEAAVEPIAVIGMACRVPGASDLGQYWSNLRDGVESIRFFD
                     REDALAAGASVRAVDDPQHVRAAPILDDIEGFDAGLFNCTRREAEILDPQHRVLLECA
                     SAALDHAGYDPGRYDGDIGVYAGVGSGEYQWYHLLPDKSLVGAVGHMAIALANNTDYA
                     ATLVAYKMNLRGPALSVSTACSTGLVAVHLACEAVRNGECDMALAGAASVELMQWHGY
                     TYHEGGILSPDGHCRAFDQDAKGTLWGSGGGVVVLKRLSDALADGDTVHGVILGSAIN
                     NDGSDKVGFSAPSVSGQSAVIRQALGVAGVHPDSIDYVEAHGTGTNVGDPIEVRALSA
                     ALGSTREPQSCLLGSVKPNIGHLAAAAGVAGLIKTVLSLENETIPPTLHFQQPNPAMN
                     LETTALRVASVKHPWTRGSRPRRAGVSSFGMGGTNAHAIVQEAPLVPIATSSGRPQIV
                     VVSGKTPSALDSAATSLAETLRAQPDLSLDDVAYTLQDGRRRLPYRRALVATDLADAA
                     AVLEGAAPRRVLVGKAPAAAEAVFLLPGQGTQRAGMAADPYAQFDVVRCWIDTGAELL
                     LPILGFDVRELLVTRFEADSPAQLAADERLRQTGTTQPALFVFEFALAKLWESWGVQP
                     KAMVGHSLGEYVACCLAGVFGFEDGVRIVATRAALMESVAPGSMIAVPMDEQSLELPG
                     TGVELAAVNAPMACVVSGPVDRIDAYEAALVEEGVPVTRLRTSHGFHTSMFEPVRAAL
                     AAAFEGVQLRSPAVPVVSNVTGTWLTAEQATDPQYWAEHMCRPVLFAAGLQTALADRS
                     SVPVEVGPGNALSGLARMQLPTDTPDPVPTLPAHSADGYVALLEAAARAWTLGLPVDF
                     TPGRATGRRRIPLPGHPFERQRYWIDGVDALVTTGPPAAAELPEGRQEPDDWYWVPTW
                     RQTAAVAAEVPAGSGPWLVLRDRAGHVDDLVARLRADGATVVTVTSGDAYAGADGDYT
                     VRCDEPGDFERLVADLVASGTTPSRVVHAMALDEVPADLLDLAAARRTVSLTFTSSLH
                     LAQALAGQGLAENLDVTFVTRDAQAVVGGDVRTPAAAVLSGPARTLPMEFAGVTCRQV
                     DVDDPGLATRAILDAVAAEEPAQVIAYRRGRRWLAGLEQAPVADTATGQARLRDRGVY
                     LLTGGLGGIGLSIAEDLARRVRARVVLLGRSPLPPRSTWAERIAAGGRQGRQLATLLR
                     IEEPGGEVLAVAADVTRPEQLADARRQVLDRYGEINGIVHAAGVAGGTMVEAHSAERA
                     ATVLEPKILGTLALAQAFQYDALDFLALCSSVTALAGGLGQLDYCAGNAFLDAFAHAA
                     DGAAPWPVLSINWGAWLEVGMAVETAVPNALLDVERGLERTIPTALERRPLAHPLLAE
                     SEQAAGSKGLVCTTELGPEQHWVLAEHRILGTPAMPGTGYLEMARAAFAQGDDSRPVE
                     LRDVVFLAPLGIPDDSSRKVRVLIEDTDGGAWRIESWAGADWQEHARGFVAWAPQTPS
                     AVTDLDAVIERCPNVRENASLQDSASGLVGFGGRWSSLQQVRGNVTEELALLVAGPDV
                     AADLPAYGLHPAVLDEATAFGDYAEASGSYLPLGYGRITVRRPMPERLYSHLRHRDSG
                     TGELLTCDITLLDEHGAVVADIGD"
BASE COUNT          720 a         2026 c         1926 g          744 t
ORIGIN      
        1 gaagccctgc cgctcaacgg gaacggcaag ctcgaccttg ccgcactgcc ccgccagcgc
       61 acgctctcgg ccgcgccggc cgccccggcc gtggcaccgg ccgacccgct cgagcagtcg
      121 atcgcccagg tctggcagac cgtcctcggc ctggcgtcgg tcggcgtcga cgacaacttc
      181 ttcgacgcgg gcggaacctc gatccgcctg gtcgctgtcc agtccggcct gcaggcgctg
      241 ggccgaccgg tgtcggtgat cgacctcttc cagtacccca cgatcaggtc cctcgcccgg
      301 cacctgtccg accgcgacaa ccgcgccgac atcggccagc cgagctccga ccggcgcatc
      361 gcggcccgcc gtggactgcg ccgccgcccc gccgaccggg tgccgccgag cgcaccgcag
      421 tccgagccat ccgcctcctg acctatcgca caccccacgt ccaaacggat caaacccgct
      481 aaaatagaac aggatcaaca tgtttggcaa cgccatgaat gacgacgagg ccgccgtcga
      541 gccgatcgct gtgatcggca tggcctgccg agtacccggc gcatcggacc tcggacagta
      601 ttggagcaac ctacgcgacg gcgtcgagtc gatccggttc ttcgaccgcg aggacgcctt
      661 ggcggccggc gcgagcgtcc gtgccgtcga cgacccgcag cacgtgcgcg cggcgccgat
      721 cctcgacgac atcgagggct tcgatgccgg cctgttcaac tgcacccgcc gcgaggccga
      781 gatcctggac ccgcagcacc gcgtgctgct cgagtgcgcg tccgcggcgc tcgaccacgc
      841 cggctacgac ccgggccggt acgacggtga catcggcgtg tacgccggag tcggctccgg
      901 cgagtaccag tggtatcact tgcttcccga caagtcactc gtcggggccg tcggccacat
      961 ggcgatcgcc ctggccaaca acaccgacta cgccgccacc ctggtcgcct acaagatgaa
     1021 cctgcgcggg cccgccctct cggtgtcgac cgcatgctcc accggcctgg tcgcggtcca
     1081 cctggcgtgt gaggccgtgc gcaacggcga gtgcgacatg gcgctcgcgg gcgcggcctc
     1141 ggtcgagctc atgcagtggc acggctacac ctaccacgag ggcggcatcc tgtcgccgga
     1201 cggccactgc cgcgcgttcg accaggacgc caagggcacg ctgtggggga gcggcggcgg
     1261 ggtcgtcgtg ctgaagcggc tgtccgacgc gctggccgac ggcgacaccg tgcacggcgt
     1321 cattctcgga tcggcgatca acaatgacgg ctcggacaag gtcgggttct cggcgccgag
     1381 cgtgtccggc cagtccgcgg tgatccggca ggcgctcggc gtcgccgggg tgcacccgga
     1441 cagcatcgac tacgtcgagg ctcacggcac gggcacgaac gtcggcgacc cgatcgaggt
     1501 gcgggcgttg agtgccgccc tcggcagcac ccgggagccg cagtcctgcc tgctcggctc
     1561 ggtcaagccc aacatcgggc acctggccgc ggccgccggg gtcgccggcc tgatcaagac
     1621 cgtcctgtcc ctcgagaacg agacgatccc gccgaccctg cacttccagc agccgaatcc
     1681 ggcaatgaac ctggagacga ccgccctgcg ggtcgccagt gtcaagcacc cctggacgcg
     1741 gggcagccgc ccccggcgag ccggcgtcag ctcgttcggc atgggcggca ccaacgcgca
     1801 cgccattgtg caggaggccc cactcgtccc gatcgcgacg tcgagcggcc ggccccagat
     1861 cgttgtcgtc tccggcaaga cgccctcggc cctcgatagc gcggcgacca gcctcgcgga
     1921 gactctgcgc gcccagccgg acctctcgct ggacgacgtg gcctacaccc tgcaggacgg
     1981 gcgccgacgc ctgccctacc ggagggcgct cgtagcaacc gatctcgccg acgcagccgc
     2041 cgttctggag ggtgccgcac ctcgtcgggt gctggtcggc aaggctccgg ccgcggccga
     2101 ggcggtcttc ctgctgcccg gccaaggcac acagcgggcc ggcatggctg ccgaccccta
     2161 cgcgcagttc gacgtcgtac gctgctggat cgacaccggc gcggagctcc tgctcccgat
     2221 cctcgggttc gacgtgcgcg agctgctcgt gacccggttc gaggccgact cccccgcgca
     2281 gctcgctgcg gacgagcggc tccggcagac cggcacgacc cagcccgcgc tgttcgtctt
     2341 cgagttcgcg ctggccaagc tctgggagtc ctggggtgtg cagccgaagg ccatggtcgg
     2401 gcacagcctc ggggagtacg tcgcgtgctg cctcgccggt gtgttcggct tcgaggacgg
     2461 cgtacggatc gtggccaccc gcgcggccct gatggagtcg gtcgcgccgg gatcgatgat
     2521 cgccgtgccg atggacgagc agagcctcga gctgccgggc accggcgtcg agctggccgc
     2581 tgtcaacgcg ccgatggctt gtgtcgtcag cgggccggtc gaccggatcg acgcctacga
     2641 ggcggccctg gtcgaggagg gcgtgcccgt cacccggctc cggacctccc acggcttcca
     2701 caccagcatg ttcgagccgg tgcgcgccgc gctggcggct gccttcgagg gtgtccagct
     2761 ccggtcgccg gcggtgcccg tcgtctcgaa cgtcaccggc acctggctga ccgccgagca
     2821 ggcgacagac ccgcagtact gggcggagca catgtgccgc ccggtgctgt ttgccgccgg
     2881 gctgcagacc gcgctggccg accggtcctc ggtgccggtc gaggtcgggc cgggcaacgc
     2941 actctccggc ctggcccgga tgcagctgcc cacggacacc cccgacccgg tccccacgct
     3001 gcccgcgcac tccgcggacg ggtacgtcgc gcttctcgag gcggcggcac gcgcctggac
     3061 cctcggcctc ccggtcgact tcactcccgg ccgggcgacc ggtcggcgcc ggatcccgtt
     3121 gcccggccac ccgttcgagc ggcagcggta ctggatcgac ggcgtcgacg cgctcgtgac
     3181 caccggccct cccgccgccg ccgagctgcc cgaagggcgg caggagccgg acgactggta
     3241 ctgggtgccg acctggcgcc agaccgccgc ggtcgcggcc gaggtgcctg caggcagcgg
     3301 gccctggctg gtgctgcggg accgggccgg gcacgtggac gacctcgtcg ctcgcctgcg
     3361 cgccgacggc gcgacggttg tcacggtgac gtccggcgac gcctacgccg gcgccgacgg
     3421 cgactacacc gtgcggtgcg acgagcccgg cgatttcgag cggctcgtgg ccgacctggt
     3481 cgcgtcgggc acgaccccga gccgggtcgt gcacgccatg gcgctcgacg aggtgccggc
     3541 cgacctgctc gacctggccg ctgcgcgccg taccgtctcg ctcaccttca cgagctctct
     3601 gcacctcgcg caggctctcg ccgggcaggg cctggcggag aacctcgacg tcacgttcgt
     3661 cacgcgcgac gcgcaggccg tcgtcggtgg cgacgtgcgc acgcccgccg ccgccgtcct
     3721 cagcggcccg gcccgcaccc tgccgatgga gttcgcgggg gtgacgtgcc ggcaggtcga
     3781 cgtcgacgac ccggggctcg cgacgcgcgc catcctggac gcggtggcgg ccgaggaacc
     3841 cgctcaggtc attgcctacc gccgcggccg ccgctggctg gccggactgg agcaggctcc
     3901 ggtcgccgac accgcgaccg gccaggcgcg gctgcgggac cggggtgtct acctactcac
     3961 cggaggcctc ggcggcatcg gcctgagcat cgccgaggac ctcgcccgcc gggtgcgggc
     4021 gcgggtcgtg ctgctgggac gttccccgct gccgccgcgc tccacctggg ccgagcggat
     4081 cgctgccggt ggccgtcaag gccgtcagct ggcgacgctg ctgcgcatcg aggagcccgg
     4141 cggggaggtg ctggccgtcg ccgccgacgt cacccgcccc gagcagcttg ccgacgcgcg
     4201 ccggcaggtg ctcgaccggt acggcgagat caacggcatc gtgcacgcgg cgggggtcgc
     4261 gggcgggacg atggtcgagg cgcacagcgc cgagcgcgca gcgaccgtgc tggagccgaa
     4321 gatcctcggc acacttgccc tggcgcaagc gttccagtac gacgcgctgg acttcctcgc
     4381 gctctgctcg tccgtgaccg ccctggcggg tggcctgggc cagctcgact actgcgcggg
     4441 caacgccttc ctcgacgcgt tcgcgcacgc cgccgacggc gccgccccgt ggccggtgtt
     4501 gtcgatcaac tggggtgcgt ggctcgaggt cggcatggcc gtcgagacag cggtgccgaa
     4561 tgcgctgctc gacgtcgagc gcgggctcga gcgcacgatc ccgacggctc tggagcgccg
     4621 ccccctcgcg cacccgctgc tcgccgagtc cgagcaggcg gcaggatcca agggcctcgt
     4681 ctgcacgacc gagctcgggc ccgagcagca ctgggtgctc gccgagcacc ggatcctcgg
     4741 cacgccggcc atgcccggta cgggctacct ggagatggcg cgggcggcct tcgcgcaggg
     4801 cgacgacagc cggccggtgg agttgcggga cgtggtgttc cttgcgcccc tcggcatccc
     4861 ggacgactcc agccggaagg ttcgggtact catcgaggac accgacggcg gcgcctggcg
     4921 gatcgagagc tgggccggtg cggactggca ggagcatgcc cgtgggttcg ttgcctgggc
     4981 gccgcagacg ccatcggccg tgaccgacct ggacgccgtg atcgagcggt gcccgaacgt
     5041 tcgcgagaac gcctccctgc aggactccgc cagcgggctg gtcggcttcg gcggccggtg
     5101 gagcagcttg cagcaggtgc gcggcaacgt gaccgaggag ctcgcgctgc tggtggccgg
     5161 gcccgacgtg gccgccgacc ttccggcgta cgggctgcac ccggcggtcc tcgacgaggc
     5221 cacggcgttc ggcgactacg ccgaggcgag cgggtcgtac ctgccgctcg gctacggccg
     5281 gatcaccgtg cggcggccga tgccggagcg gctgtacagc cacctgcggc accgcgactc
     5341 gggcaccggc gagctgctca cctgtgacat cacgctgctc gacgagcacg gtgcggtcgt
     5401 cgccgacatc ggcgac
//
LOCUS       DATAIX010000224         2059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_213812, whole genome shotgun sequence.
ACCESSION   DATAIX010000224 DATAIX010000000
VERSION     DATAIX010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213812"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="VHM65_03345"
     CDS             <1..1354
                     /locus_tag="VHM65_03345"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEX2704768.1"
                     /translation="RTHNGTQVLMNHELASDKGIVRRHGQEGSFVSKVSLDRTGRVVK
                     AEDFITSVRYFSFGSFGDGLGTYQATPVGTDDARFNRFCSSTLAAAGSLYNRATGRGT
                     TERIYFANEEGNDESRVFGVDESGHAVELPRLGRFATENSVPAAHSGNTTVVMGNEDG
                     ATTTSELRVYVGKKERGFGKSPFAKAGLQNGTSHVIAVTDGVGGLVPSDAAFRAAYGK
                     GDKVRFSLSEVPWDATGAAQNAEADVEGTSFDRIEDGQWDPRKRNVYYFVTTQGGEGT
                     VQGAGNGGGGGLWRLTYDDVRNPSAGGTLELLLDGTEAINLVKPDNMTIDGRGNILLQ
                     EDPGNIDAVARIAAYRIADGALGQIATFDPALFTPGAPRFLTKDEESSGIIDLEPLGY
                     PAGTFLFDAQVHTDLGLPADPDPAADNPGTADEYVERGQLLKLGVADFRSVYTILPVA
                     "
     gene            complement(1532..1807)
                     /locus_tag="VHM65_03350"
     CDS             complement(1532..1807)
                     /locus_tag="VHM65_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704769.1"
                     /translation="MSAAVATSLRTLTEIWRGDGSWDRALRSGRVVVTAAEHVRRAVP
                     QWLGQSTLAATPRPALPSNGDAAESVAPQRHLLSVSAAVPGSPQTRP"
     gene            complement(1845..>2059)
                     /locus_tag="VHM65_03355"
     CDS             complement(1845..>2059)
                     /locus_tag="VHM65_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704770.1"
                     /translation="GRELAGVVEGLGAWGVRWIHEIGDEDLDPHLLMWDMRRTVPVHT
                     WPRSRTVVSVRRRRAPGGEVVARRLR"
BASE COUNT          319 a          751 c          700 g          289 t
ORIGIN      
        1 ccgcacgcac aacggcactc aggtgctgat gaaccacgag ctggcgtccg acaaaggcat
       61 cgtgcggcgc catggccagg agggctcctt cgtgtcgaag gtgtcgctcg accgcacggg
      121 gcgggtggtc aaggctgagg acttcatcac ctccgtccgg tacttcagct tcggttcctt
      181 cggggacggt ctcggcacct accaggccac gccggtcggc accgacgacg ctcggttcaa
      241 ccgcttctgc tcttcgactc tggcggccgc cggctcgctg tacaaccggg ccaccggtcg
      301 gggcaccacc gagcgcatct acttcgccaa cgaagagggg aacgacgagt cgcgggtgtt
      361 cggcgtcgac gagtcgggcc acgctgttga gcttccccgc cttggccggt tcgcgacgga
      421 gaactcagtg cccgccgccc actcgggcaa caccaccgtc gtgatgggca acgaggacgg
      481 tgccaccacc acctctgagc tgcgggtgta tgtcggtaag aaggagcgcg gcttcggcaa
      541 gagcccgttc gccaaggccg gcctccagaa cggcacgagc cacgtgatcg cggtgaccga
      601 cggcgtcggc ggcctcgtgc ccagcgatgc cgcgttccgt gccgcgtacg gcaagggcga
      661 caaggtgcgg ttctcgctgt ccgaggtccc gtgggacgcc accggagcgg cccagaacgc
      721 cgaggcggat gtggagggga cctccttcga ccgcatcgag gacggccagt gggatccccg
      781 caagcggaac gtctactact tcgtcaccac ccagggcggc gaagggacgg tccaaggcgc
      841 cggcaacggc ggcggcggtg gcttgtggcg gctgacctac gacgatgtcc gcaacccctc
      901 cgccggcggc accctcgagc tcctgctcga cgggaccgag gcgatcaacc tggtcaagcc
      961 ggacaacatg acgatcgacg gtcgcggcaa catcctgctc caggaggacc cgggcaacat
     1021 cgacgctgtc gcccgcatcg cggcgtaccg catcgccgac ggtgccctcg gccagatcgc
     1081 caccttcgac ccggcgctgt tcaccccggg ggctccccgc ttcctcacca aggatgagga
     1141 gtccagcggc atcatcgatc tggagccgct cggctacccg gccggcacgt tcctgttcga
     1201 tgcgcaggtg catacggacc tcggccttcc cgccgacccc gacccggccg ccgacaatcc
     1261 cgggacggcc gacgagtacg tggagcgagg ccagctcctc aagctcggtg tcgccgactt
     1321 ccggtcggtg tacacgatcc tgcctgtcgc ctagccagct cgatcaccgt cacgacgtga
     1381 aggggcgggt cgccggtctc ggtgacccgc ctcgtcacgt tcggccatcg ggctgtccgg
     1441 ccacgccctt caccagcgct cgtcgacccc ggtggtcgga ggcggcgacg atggcggttc
     1501 agcaggtcga ggacgccggt gccgactgcg ctcagggccg cgtctgcggc gacccgggca
     1561 ccgcagcgga gacggacaag aggtggcgtt gcggcgccac ggactccgcg gcgtcgccgt
     1621 tcgacgggag cgcggggcgg ggggtggccg cgagggtgct ctggcccagc cactgcggga
     1681 cggcgcggcg cacgtgctcg gccgccgtca ctaccaccct gcccgagcgc agggcccggt
     1741 cccacgaccc gtcgccgcgc cagatctcgg tgagcgtgcg caggctcgtc gcgaccgcgg
     1801 ccgacacctc gaacccgggg tcggtgtcgc agacgtcgac gtcgtcaccg gagacgacga
     1861 gccaccacct cgccgcccgg cgctcgacgt cgtcgaaccg acaccaccgt gcgagagcgt
     1921 ggccacgtgt ggacgggcac cgtgcgccgc atatcccaca tgagcaggtg cgggtccagg
     1981 tcctcgtccc cgatttcgtg gatccagcga acgccccagg ccccgagccc ctccaccacg
     2041 ccggccagct cccgaccgg
//
LOCUS       DATAIX010000225         1563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_72251, whole genome shotgun sequence.
ACCESSION   DATAIX010000225 DATAIX010000000
VERSION     DATAIX010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72251"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1457
                     /locus_tag="VHM65_03360"
     CDS             <1..1457
                     /locus_tag="VHM65_03360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEX2704771.1"
                     /translation="LRPLSGAPTWLVADGRPREATGTWGWQPCVAEGVDVTQGRVLGT
                     LPPRGGPEFRVLVPPGVAGHVEWLAPPGTYGALDPLAVVAGTPVSLTTDWPVRRPRPV
                     RERLASSEPLLTGQRVLDAVFPLARGGTAGVPGGFGTGKTMLLQQIAKWCAAEVIVYV
                     GCGERGNEMADVLAELAELSDPRTGRRLSERTVVIANTSNMPMMAREASIYTGVTVAE
                     YFRDMGHDAVVIADSTSRWAEALREFASRAGALPAEEGYPASLPSALAAFYERAGRVT
                     TLGGESASVTVIGAVSPPGGDTTEPVTAHTQRFVRAVWGLDRELAYARHYPAVSWAGS
                     FSRDAEQLGRWYAHHGDPEWGRRRAQVTGLLAEADRLGDLAALVGTGSLPGHERVVLL
                     AGRLLREGVLQQNALSPNDAHCTAEKTAALIDAVLAVIDEAEAAVGRGVLASEVEELD
                     FSPLLRAAAESPPDETDDVVARQEQMRAQLRGLR"
BASE COUNT          188 a          586 c          582 g          207 t
ORIGIN      
        1 tgctgcggcc cctgtcgggg gcccccacct ggctggtggc ggacggtcga cctcgtgagg
       61 cgaccggcac ttggggctgg cagccgtgcg tcgccgaggg cgtcgacgtg acgcagggcc
      121 gggtgctcgg aacgctcccg ccccgaggcg gtcccgagtt ccgggtgctg gtgccgcccg
      181 gggtcgccgg ccacgtggag tggctggccc cacccggcac ctacggggcc ctcgacccgc
      241 tcgccgtcgt ggccgggacc cctgtctcgc tcaccaccga ctggccggtg cgccgccccc
      301 gtccggtccg ggagcgactg gcctcgagcg agccgctcct caccgggcag cgggtgctgg
      361 atgccgtgtt ccccctcgcc cgcggcggca ccgcgggcgt gccgggtggg ttcggcacgg
      421 gaaagacgat gctcctgcag cagatcgcca agtggtgcgc cgctgaggtc atcgtctacg
      481 tcgggtgcgg cgagcgcggc aacgagatgg ccgacgtcct ggccgagctg gccgagctgt
      541 cggacccgcg aaccgggcgc cggctgtccg agcgcaccgt ggtgatcgcg aacacctcga
      601 acatgccgat gatggcccgc gaggcgagca tctacaccgg cgtcacggtg gccgagtact
      661 tccgggacat gggccacgac gccgtggtca tcgccgactc gacgtcccgg tgggccgagg
      721 cgctgcgtga gttcgcctcc cgcgcaggcg cgctgccggc cgaggagggc taccccgcat
      781 cactcccctc ggcgctcgcg gcgttctacg agcgggccgg gagggtcacc accctggggg
      841 gcgagagcgc gtcggtgacc gtcatcggtg ccgtctcccc acctggcggc gacaccaccg
      901 agccggtcac cgcccacacc cagcgcttcg tgcgcgccgt gtggggcctc gaccgcgagc
      961 tcgcgtacgc ccggcactac cccgccgtgt cctgggccgg ctccttctcc cgcgacgccg
     1021 agcagctagg gcgctggtac gcgcaccacg gggacccgga gtggggcagg cgacgggcgc
     1081 aggtgaccgg gttgctcgcc gaggccgacc ggctcggcga cctcgcggca ctggtcggca
     1141 cggggtcgct gccgggccac gaacgggtcg tcctcctcgc cggccgcctc ctgcgcgagg
     1201 gggtgctgca gcagaacgcc ctgtcgccca acgacgccca ctgcaccgcc gagaagaccg
     1261 ccgcgctcat cgacgcggtc ctcgccgtca tcgacgaggc cgaggccgcc gtgggtcgtg
     1321 gcgtgctcgc ctccgaggtg gaggagctcg acttctcgcc gctgctgcgc gcggccgcgg
     1381 agagcccgcc cgacgagacc gacgacgtcg tcgcccgcca ggaacagatg cgggcacagc
     1441 tcagggggct gcgatgacga gctcgccgtc caccggcagc cgcgcgccca ccttcgaggt
     1501 cgcctacgcg gacatcgtgg agatgcacgg gccgctcctg ctcgtccgcg gggtgtgcgg
     1561 ggt
//
LOCUS       DATAIX010000226         1766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_42264, whole genome shotgun sequence.
ACCESSION   DATAIX010000226 DATAIX010000000
VERSION     DATAIX010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42264"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1653
                     /locus_tag="VHM65_03365"
     CDS             <1..1653
                     /locus_tag="VHM65_03365"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX2704772.1"
                     /translation="DMFLVMELVPGRTLRQVLADEGALTPRAALTIIEQVLQALDAAH
                     RAGLIHRDIKPENVMLDERMTVKVTDFGLARAVTTTTMIGQPGVLLGTASYLAPEQVE
                     RGVADARSDVYAVGLLLFEMLTGSKAVSGDNPIHVAYQHVHGSVPAPSSRVAGVPATL
                     DALVAHATSRDPDDRPRDAGAMLADLRNRRADMSTIELDLRPAGAQAMLPPAPTVAVE
                     RTTALPVSAPTSDTLEGAYLPSVPGPVGSAAAHSSQGAQRRGRVGWVTAALVLALVVV
                     GGWFFTLGPAGTTTVPTTVGLSRDAADSALQAAGLTAEVTEAFDETAPKDQVMTSDPA
                     SGQSARKGTAVSLTVSKGPERYSAPTLVGTKADGAKAALAAVKLALGEVKAQYDEKVA
                     AGVVISQAPAAGQPLKPGATVSIVVSKGREPIPVTDYTGKPADAATAALTKLGLTVTR
                     GEAVNSDTVPQGSIISQSPKSGTLFKGATVTIVVSKGPVLIEVPGVVGMQRADARRIL
                     ERAGFTVRFDEVLGGFFGTVRSQDPAGGSKAARGTTVSLVIV"
BASE COUNT          255 a          670 c          589 g          252 t
ORIGIN      
        1 gacatgttcc tcgtcatgga gctcgtgccc ggccggaccc tgcgccaggt cctcgccgac
       61 gagggagcgc tgacgccgcg agcggccctg acgatcatcg agcaggtgct ccaggccctc
      121 gacgccgcgc accgcgcggg actgatccac cgggacatca agcccgagaa cgtcatgctc
      181 gatgagcgga tgacggtcaa ggtgaccgac ttcggtctcg cgcgggctgt gacgacaacg
      241 acgatgatcg gtcagccggg cgtcctgctc ggcaccgcgt catacctcgc tccggagcag
      301 gtcgagcgcg gcgtcgccga tgcccgcagc gatgtgtatg ccgtcgggct gctgctcttc
      361 gagatgctca ccggttccaa ggcggtctcc ggggacaacc cgatccacgt ggcctaccag
      421 cacgtccatg ggtcggtgcc ggctccgtcc tcccgcgtcg ccggcgtgcc cgccaccctc
      481 gacgcgctcg tggcgcatgc gacctcacgc gacccggacg accgcccgcg cgacgccggc
      541 gcgatgctgg ccgacctgcg caaccgccgc gccgacatgt cgacgatcga gctcgacctg
      601 cgaccggccg gcgcccaggc gatgctcccg ccggccccga cggtcgcggt cgagcgcacg
      661 actgccctgc ccgtatcggc tccgacatcg gacacgctcg aaggggccta cctgccttcg
      721 gtcccgggcc cggtggggag cgctgcggcc cactcgtccc agggcgccca gcgccggggt
      781 cgcgtcggct gggtcactgc cgcgctggtt ctcgcgctcg tcgtcgtagg cgggtggttc
      841 ttcaccctcg gaccggctgg gacgactacc gtccccacca ccgtcggcct gtcccgggac
      901 gccgccgaca gcgcgctcca ggcggcgggc ctgacggccg aggtcaccga ggcgttcgac
      961 gagacggccc ccaaggacca ggtcatgacg tcggacccgg catcggggca gtcggcccgc
     1021 aagggcacgg ccgtctcgct cacggtgtcc aagggccccg agcgctactc ggccccgacc
     1081 ctcgtcggca cgaaggccga cggcgccaag gccgcgctcg cggcagtcaa gctcgccctc
     1141 ggcgaggtca aggcgcagta tgacgagaaa gtagccgcgg gggtggtcat ctcgcaggcc
     1201 cccgcggcgg gtcagccgct caagccggga gccaccgtct cgatcgtcgt gagcaagggc
     1261 cgcgagccga tcccggtgac cgactacacc ggcaagcccg ccgatgccgc caccgctgcg
     1321 ctgaccaagc tcggcctcac ggtcactcgg ggcgaggccg tcaacagcga caccgtcccc
     1381 cagggctcga tcatcagcca gagccccaag agcggcacgc tgttcaaggg cgcaacagtg
     1441 acgatcgtcg tctccaaggg ccccgtgctc atcgaggtcc ccggggtagt cggcatgcag
     1501 cgcgccgacg cacgccgcat cctcgagcga gccggattca ccgtgcgctt cgacgaggtg
     1561 ctgggcggct tcttcggcac ggtccgctcc caggatcccg cgggcgggtc gaaggccgca
     1621 cgaggcacca ccgttagtct cgtcatcgtc taggtcctga tggcccccca agcgctccgt
     1681 gggcagtccc ttgccaccat cctgctcgtc gccctgaccg ccatctgggg gtcgaccttc
     1741 ttcctgatcc gtgacctcgt cacggt
//
LOCUS       DATAIX010000227         3393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_42437, whole genome shotgun sequence.
ACCESSION   DATAIX010000227 DATAIX010000000
VERSION     DATAIX010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42437"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VHM65_03370"
     CDS             complement(<1..961)
                     /locus_tag="VHM65_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEX2704773.1"
                     /translation="MSSTTAPEGAASLRADDPASSAPAGEGMTRIGGVAGWVDERTGV
                     AKPVAHLLKKVFPDHWSFMLGEIAMYSMIICLLTGAFLTFWFVPSAGQISYDGSYLPL
                     QGVTMSEAYASTLGISFDIRGGLLIRQIHHWAALLFVVAIVVHMMRVFFTGAFRKPRE
                     VNWVIGCILSMLAIVEGFAGYSLPDDLLSGTGLRAAEGFMQSIPVIGSYVAYIAFDGP
                     FPGELIIPRLFSLHVLLIPAILVGLFTAHIVLVFVHKHTQYPGPGRTNSNVVGFPFMP
                     VYIAKAGGFFFIVFGVTALISALVTINPIWAYGPYDPSPVTAGS"
     gene            complement(961..2049)
                     /locus_tag="VHM65_03375"
     CDS             complement(961..2049)
                     /locus_tag="VHM65_03375"
                     /inference="COORDINATES: protein motif:HMM:NF039285.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX2704774.1"
                     /translation="MNEHTTTGSTLDLAHGDHHGTGGLPDRFADPGLPPHVARQADLN
                     EKAAKRAERQVASLFAMSIAGTLLFLVAFFTIDDATASFVPFMGKVNVYNIVIGLGMA
                     LSLFGIGAGAVHWAKTLMPDHEIAEERHPLRSSDESRQDAVDTTLGGGEAAQLGRRPL
                     IKATLGGALGLFALPFVVQVVRSLGPATSKDELFHTIWDIPTEGPAAGKPIRLMRDPL
                     MTPIKAEEVTIGSVFHVMPETLQKYLDEGHHVLEEKGKAAVLLMRLDPPDITSQKAKD
                     WGYQGIVAYSKICTHVGCPVGLYEQQTHHLLCPCHQSTFDVTKDCEVIFGPAKRPLAQ
                     LKITVDAEGYLVATQGFREAVGPSFWER"
     gene            complement(2059..2832)
                     /locus_tag="VHM65_03380"
     CDS             complement(2059..2832)
                     /locus_tag="VHM65_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX2704775.1"
                     /translation="MAARRRHPAAIALLLLLGLLVTGGAYSLLAPKPAKAAVMSADEI
                     AKGKNLFLANCATCHGLSAQGTQTGPSLYGVGAAAVDFQVSTGRMPMVVPGVQAARSE
                     VKFSEEEIAAMAAYAASLAPGPAVPDPATVTGEDANAAAGGELFRVNCAMCHNFAGAG
                     GALTRGKYAPALAGATGTQVYEAMVTGPSSMPVFSDTNMDLKAKNDVIAYLTAIENEQ
                     QVGGFNLGNLGPVSEGFFVWIFGLGLLIACAVWLGRKAA"
     gene            complement(2914..>3393)
                     /locus_tag="VHM65_03385"
     CDS             complement(2914..>3393)
                     /locus_tag="VHM65_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HEX2704776.1"
                     /translation="LWAESTAKLNITYAAANTLILVISSVWCQMGVFAAERGQPGRSG
                     TLLQWRKWGMREWYVLAYLFGAVFVAGQVYEYANLISENISLSSNAYGSVFYMTTGFH
                     GMHVTGGLLAFLLIVGRSFATRHYSHTQAIGAVVISYYWHFVDVVWIALFATIYILK"
BASE COUNT          584 a         1040 c         1235 g          534 t
ORIGIN      
        1 gggaacccgc ggtcacgggg gacgggtcgt agggtccgta ggcccagatc gggttgatcg
       61 tcaccagcgc cgagatgagt gcggtgacgc cgaagacgat gaagaagaag ccgccggcct
      121 tcgcgatgta gaccggcatg aacgggaaac ccacgacgtt gctgttcgtg cgaccggggc
      181 cggggtactg cgtgtgcttg tgcacaaaga cgaggacgat gtgcgccgtg aacagcccga
      241 ccaagatggc cgggatcagc agcacgtgca ggctgaacag ccgcgggatg atcagctcgc
      301 ctgggaaggg cccgtcgaag gcgatatagg cgacgtagga gccgatcacc gggatcgact
      361 gcatgaagcc ctcggcggcc cgcaggccgg tgccggagag caggtcgtcg ggcagcgagt
      421 agccggcgaa gccctcgacg atcgcgagca tcgacaggat gcagccgatg acccagttga
      481 cctcgcgtgg cttgcggaac gcgccggtga agaagacgcg catcatgtgc acgacgatgg
      541 cgacaacgaa cagcagcgcc gcccagtggt ggatctggcg gatgagcaga ccaccacgga
      601 tgtcgaagga gatgcccagg gtcgaggcgt aggcctcgct catggtgacg ccctgcagcg
      661 ggaggtagga cccgtcatac gagatctggc cggcgctcgg cacgaaccag aaggtgagga
      721 aggcgccggt gagcaggcag atgatcatcg agtacatcgc gatctcgccg agcatgaagc
      781 tccagtggtc cgggaagacc ttcttgagca ggtgagcgac cggcttggcg acgccggtgc
      841 gctcgtccac ccacccggcc acgccgccga tcctcgtcat gccctcgccg gccggcgccg
      901 aggacgccgg gtcgtcggca cgcagcgacg cggctccctc gggtgcagtt gtgctgctca
      961 tcagcgctcc cagaaactcg ggccgacggc ttccctgaag ccttgggtgg cgacgaggta
     1021 gccctcggcg tcgacggtga tcttcagctg cgccagaggc cgcttggcgg ggccgaagat
     1081 cacctcgcag tccttggtga catcgaaggt cgactggtgg caggggcaga gcagatggtg
     1141 ggtctgctgc tcgtacaagc ccacggggca gccgacgtgg gtgcagatct tggagtaggc
     1201 cacgatgccc tggtagcccc agtccttggc cttctgcgag gtgatgtcgg gggggtcgag
     1261 acgcatgagc aggacagcgg ccttgccctt ctcctccagg acgtgatggc cctcgtcgag
     1321 gtacttctgc agggtctcgg gcatgacgtg gaagaccgac ccgatcgtca cctcctcggc
     1381 cttgatcggg gtcatgaggg ggtctcgcat gagccggatc ggcttgcctg cggcggggcc
     1441 ttccgtgggg atgtcccaga tcgtgtggaa cagctcgtcc ttgctcgtcg ccgggcccag
     1501 cgagcgcacg acctggacga cgaacgggag ggcgaacagg ccgagtgcgc cgcccagggt
     1561 ggccttgatg aggggacggc gaccgagctg ggccgcctcg ccaccgccga gggtggtgtc
     1621 cacggcgtcc tggcggctct cgtcggacga gcgcaggggg tgtcgctcct cggcgatctc
     1681 gtggtcgggc atcagtgtct tggcccagtg gaccgcgccg gcgccaatgc cgaacaggct
     1741 cagggccatt ccgaggccga tgacgatgtt gtagacgttg accttgccca tgaacgggac
     1801 gaaggaggcg gtcgcgtcgt cgatcgtgaa gaacgcgacg aggaacagca gcgtgccggc
     1861 gatcgacatc gcgaacaggc ttgcgacctg acgctcggcc cgttttgccg ccttctcgtt
     1921 caggtccgcc tggcgtgcga cgtgcggcgg cagcccgggg tcggcgaacc ggtcgggaag
     1981 cccacccgtg ccgtggtggt cgccgtgggc aaggtcgagg gtcgaccccg tggtcgtgtg
     2041 ctcgttcacg ctcattcctt atgccgcctt cctgccgagc cacaccgcgc aggcgatgag
     2101 caggccgagc ccgaagatcc acacgaagaa cccttcggac accgggccga ggttgccgag
     2161 gttgaacccg cccacctgct gctcgttctc gatggccgtg aggtaggcga tgacgtcgtt
     2221 cttggccttg aggtccatgt tggtgtcgct gaagaccggc atcgacgacg ggccggtcac
     2281 catggcctcg tagacctggg tcccggtcgc gcccgccagc gcgggagcgt acttgccacg
     2341 ggtcagcgcg ccgcctgccc cggcgaagtt gtggcacatg gcgcagttga cccggaacag
     2401 ctcaccgccg gccgcggcgt tggcgtcttc gccggtgacg gtcgccgggt cggggacggc
     2461 cggacctggg gccagcgagg ccgcatatgc ggccatcgcc gcgatctcct cctcggagaa
     2521 cttcacctcg ctgcgcgccg cctggacgcc gggcaccacc atcggcatgc ggccggtgga
     2581 gacctggaag tccacggcgg cagccccgac gccatacagc gaaggtccag tctgcgtccc
     2641 ctgggcactg aggccgtggc aagtcgcaca gttggcgagg aagaggttct tgcccttggc
     2701 gatctcgtcc gctgacatga cggctgcctt ggccggcttg ggagcaagca ggctgtaggc
     2761 cccgccggtc acgaggagcc cgagaagcag gagaagggcg atggcggccg ggtgacgtcg
     2821 gcgggcggcc atggcgttca cggtgctgtc ctgtcgtcgg ttgtcgagta gtggcgaacc
     2881 taccagcagg tcaggccggg gcgcagcagc gggtcacttg aggatgtaga tcgtggcgaa
     2941 cagggcgatc cacacgacgt ccacgaagtg ccagtagtac gagatcacga cggcgccgat
     3001 ggcctgagtg tggctgtagt gcctcgtcgc gaaggaacgg ccgacgatga gcaggaaggc
     3061 gagcagaccg ccggtgacgt gcatgccgtg gaatccggtg gtcatgtaga agaccgagcc
     3121 gtaggcgttg gacgacaggc tgatgttctc ggagatgagg ttggcgtact cgtagacctg
     3181 tccggcgacg aagacggcgc cgaagaggta ggcgaggaca taccactcgc gcatgcccca
     3241 cttccgccac tggagcaggg ttccgctacg tccgggctgg ccgcgctcgg ctgcgaacac
     3301 gcccatctgg caccacaccg aggagatcac gaggatcagg gtgttggcgg ccgcgtaggt
     3361 gatgttgagc ttggccgtcg actcggccca gag
//
LOCUS       DATAIX010000228         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_273749, whole genome shotgun sequence.
ACCESSION   DATAIX010000228 DATAIX010000000
VERSION     DATAIX010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273749"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..369)
                     /locus_tag="VHM65_03390"
     CDS             complement(163..369)
                     /locus_tag="VHM65_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704777.1"
                     /translation="MRCPSAAASSSSILSAGTTASTSKESEGGQLVGLASDSVAQVAA
                     PVRPRVVGYGAPTGRGERRYEQQG"
     gene            609..1409
                     /locus_tag="VHM65_03395"
     CDS             609..1409
                     /locus_tag="VHM65_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX2704778.1"
                     /translation="MCSLKGGVGKTSVVLGLASAALAAKVPTLVVDLDPQGDATTALD
                     VTEPPASHVAMVLDRPSAEIVRSAVGPSSWSRDWPGSRLDVLVGSPDAARHDHPYPSA
                     TALGRLRSALSRTEGYDLVLIDCPPSLGGLTRTGLVASQRALVVTEPGLFALQAADRV
                     LATIDIVRKDHAPDLQPLGIIANRVRSRTTEHAFRLEELASMFGPLFLNPFIPERAAV
                     QQAQGALGPIHGWPSAGAREMAQRFDALLARALRAPAARVRGESGATR"
     gene            2190..>2630
                     /locus_tag="VHM65_03400"
     CDS             2190..>2630
                     /locus_tag="VHM65_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2704779.1"
                     /translation="MRAQLGLVSANRDFRVLLGAFVVQALAIGCMLAGVDYVARQVLH
                     NRSAATLLFVCFVGPALVVTPLWQRLGGPRGKKRGYVIASLLLGGGALVLTAARTLPV
                     TVVYLAVAVVGIGYAGAQLFPLAMLPDVAALDAARSTENRVGVFT"
BASE COUNT          283 a         1001 c          902 g          444 t
ORIGIN      
        1 gccatagcgt ggcgtcatgg tgccgtggcg tcatggtgcc atgacgccac ggcaccatga
       61 agccctggca caccggtata acgcgcagca ggcccgcgtt cgccgcccgc tcgccatacc
      121 cctgctcgcc ataccctgct cgcatagctc ctgctgctcg ccctacccct gctgctcgta
      181 gcggcgttcg ccacggccgg ttggcgcgcc gtagcctacg acgcggggac ggaccggcgc
      241 agcgacctgc gcgacactgt cgctcgccag gccgacgagc tggccaccct cggactcctt
      301 cgacgtgctc gcggtggtcc cggcactgag gatgctgctg ctgctggcgg ccgcggaggg
      361 acaacgcacc cttcgacgac acgacccggg cgctcgccgc tgcggcccgg gtcgagcccg
      421 tcatcgcttc ggggtctcgg cacggcagcg ggatgttccg ggaccatccc gaactggtgg
      481 cccaggtcgt cgcatttgcc ggccgatccg tcccggccgc accgtgatgc tggtgctcgg
      541 aacgcccgtg ccgcgccaac cgatgggctt gcatgggtga gtaggttggg catgtgctcg
      601 tgctcagcgt gtgcagtctc aagggaggcg tgggtaagac ctcggtcgtg ctgggcctcg
      661 cctcggcagc tctggccgcg aaggtcccga ccctcgtcgt cgacctggac ccccagggcg
      721 atgccacgac cgcgctcgac gtcaccgagc cccctgcctc acacgtcgcg atggtcctcg
      781 accggcccag tgccgagatc gtccgctcgg cggtcgggcc gagctcctgg tctcgggact
      841 ggccgggctc gcgtctcgac gtgctggtcg gaagtccgga cgcggcccgg cacgaccacc
      901 cctacccgag cgccaccgcc ctgggccggc tccgttcggc gctgagccgg accgagggtt
      961 acgacctcgt gctcatcgac tgcccgccct cgctcggagg gctgacccgc accggtctcg
     1021 tcgccagcca gcgtgccctg gtcgtcaccg agcccgggct cttcgccttg caggccgccg
     1081 accgggtgct cgcgacgatc gacatcgttc gcaaggacca cgcccccgac ctgcagccgc
     1141 tgggaatcat cgccaaccgg gtccggtcgc gcaccaccga gcacgccttc cggctcgagg
     1201 agctcgcgtc gatgttcggc ccgctgttcc tgaacccgtt catccccgag cgcgcggccg
     1261 tccagcaggc ccagggcgcg ctgggtccga tccacggctg gccctcggcg ggtgcccggg
     1321 agatggccca acggttcgac gccttgctgg cccgcgccct gcgcgcgccc gccgcccggg
     1381 ttcggggcga gtcgggtgct acccgctgag gccgggtgct gacggcgcaa gcgctggccc
     1441 ggctggcccg gctcgtccgc ggtggccagc gcacgtgcac gggctggcgt tcctgccgtg
     1501 tgccgcgcgt tcgcccccta cagtcgccgg tatgacgacg acgtcacctc gcgcgggtct
     1561 gcccgcgtcg gtcctgcggg gttatgccct cggcggggtc gccaccggct cgttcgggac
     1621 cgtgcccggg ttgctcctgc tgccctacct caccgagcgg ctcggcatcg gtgctgcgct
     1681 ggccggggtc atcgtcttcg cccccaaggc ctgggacgtc gtcctcaacc cggtcgccgg
     1741 gcggatcagc gaccgctcga cgcatccggc cggcccgcgg cggcccttcc tcatccgggc
     1801 cggcctggct ctcgcgctct gcttcgtcct gctctttgcc gggccctccg caccgccggg
     1861 cctcgctgcg gcatacgtcg cggttctctt cctcgggtgc gcgacggcct atgcgttctt
     1921 ccaggtgccg tatgtcgcca tgccggccga gatcaccgac gactacgccg aacggactcg
     1981 gatcatgacc tggcgggtcg tcgtgctcgc cctcgcgatc ctgctcagcg gggccagtgc
     2041 cccggcgatc cgggatgtgg tcgggccgga gcgcggctat ctcgtcatgg gtctggccat
     2101 cggtctgctc atcgcggcgg gcgccgtcgg agcatggtgg ggaacccggc aggctccggt
     2161 gcggcgcctc gccgtagcgg gccggcggac tgcgggccca gctggggctg gtgagcgcca
     2221 accgcgactt ccgtgtcctg ctcggggcct tcgtggtcca ggcgctcgcc atcggctgca
     2281 tgctcgccgg agtcgactac gtcgctcgcc aggtcctgca caaccggtcc gcggcgaccc
     2341 tgctcttcgt ctgcttcgtc ggtccggccc tggtcgtcac cccgctctgg cagcggcttg
     2401 ggggcccccg cggcaagaag aggggctatg tcatcgcctc gctgctcctc gggggcggtg
     2461 ccctcgtgct caccgcggcc cggacgctgc cggtcacggt cgtctacctc gccgtcgctg
     2521 tcgtggggat cggttacgcc ggtgcgcagc tgttcccctt ggccatgctg cccgacgtcg
     2581 ccgcactcga cgctgcccgc agcactgaga accgggtcgg ggtcttcacg
//
LOCUS       DATAIX010000229         3263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_42476, whole genome shotgun sequence.
ACCESSION   DATAIX010000229 DATAIX010000000
VERSION     DATAIX010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42476"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..541)
                     /locus_tag="VHM65_03405"
     CDS             complement(50..541)
                     /locus_tag="VHM65_03405"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HEX2704780.1"
                     /translation="MKIVRAIDYPASPHVVFLMLSDEAFHERKCQAAGALEHFVTITT
                     TGAGASIITARVMSTDGLPDFVRSMVGARITILETYHWGPADASGNRIGSLVVNVGDA
                     PLAMTANVDLAPADAGTVMTIDGHLRARIPLFGSKVEVAAAPVIDAAIRVEHRTGQAW
                     LAG"
     gene            complement(570..1082)
                     /locus_tag="VHM65_03410"
     CDS             complement(570..1082)
                     /locus_tag="VHM65_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704781.1"
                     /translation="MSTPEPYGDPASCSELGGTLRRAAARLGDGRDALRPGLAGLGRR
                     DGRAASRVADELALLDRTIECLDEAGAALQRYATELAELGEAARVRRARVEAGGLVLE
                     QWRVSEPWGPSGVDDAMARREAVPALQQQADQLHARIARARALLLRTVSDLDGALSRQ
                     SARVRQGLGR"
     gene            complement(1079..1393)
                     /locus_tag="VHM65_03415"
     CDS             complement(1079..1393)
                     /locus_tag="VHM65_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704782.1"
                     /translation="MDPGAAPAGSPRATGVARNLGVLRQELLRVADELLQALPDVGEH
                     TAQRALDAFVEQASDTARAHADAVEDALVTHGPAWGTQPAAGPRRPAAPARHTPLEPW
                     LR"
     gene            1549..>3263
                     /locus_tag="VHM65_03420"
     CDS             1549..>3263
                     /locus_tag="VHM65_03420"
                     /inference="COORDINATES: protein motif:HMM:NF045284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704783.1"
                     /translation="MTTSLEQSRAQLISTAAHLGQRSGGPDLEAFLGRYYRHVATDDL
                     LARAPEDLLGAALSHRELARERAVGSVNAAAFNPSVDAQGWSSGHTVVQVVCDDMPFL
                     VDSVTSELGRESRAVHLVVHPLLVARRTIAGHLEEILDLDPPGPHEFGTGVESWIHVE
                     IDRASSAAELEEIAARLTEVLGNVRDAVEDWPKMRERVRSIADGLRHGGPAGIPQREV
                     DETVAFLDWMVDDRFTLLGYREYALEREEDHDLLRTQPSSGLGLLRYDTSQSGSFARL
                     GPLVQAKAREKRLLIITKANTRATVHRSVYLDYVGVKVFDDAGEVVGEQRILGLFASS
                     AYNASVLDIPIMADRVNAVLHLAGFTHDSHSGKDLLQVLESYPRDELFQTSVEKLLDV
                     ASSVLHAQERRKTQLFLRKDDYGRFMSCLVFIPRARYNTPVRLKIESLLREAFHAVSV
                     DYTTRVTESSLARLHFVVRVRQGEVIPDVDEPGLTRAIVEAARTWDEDLAEAARTEYG
                     EEGAARLMGLYSRAFPEAYKEDFGPRVAVADVRFIESLTALDSTGLNMYQEPGAPSHE
                     RRFKL"
BASE COUNT          477 a         1175 c         1144 g          467 t
ORIGIN      
        1 ggccggtgct ggtcggccgg gtcggcctcg gtcggtgggg ggtgggttct cagccggcga
       61 gccaggcctg cccggtgcgg tgctcgacgc ggatggcggc atcgatgacg ggggccgcgg
      121 ctacttcgac cttgctgccg aagagcggga tcctggccct gaggtgcccg tcgatcgtca
      181 tgacggttcc ggcgtcggcc ggggcgaggt cgacgttggc ggtcatggcg aggggagcgt
      241 ccccgacatt gacgacgagc gagccgatgc ggttcccgct cgcgtcggcc gggccccagt
      301 ggtaggtctc caggatcgtg atccgtgccc cgaccatcga cctgacgaag tccgggaggc
      361 cgtcggtcga catcacccgg gccgtgatga tgctcgcgcc ggcaccggtg gtcgtgatcg
      421 tcacaaagtg ctcgagggcc cccgcggcct ggcacttgcg ctcatgaaag gcctcgtcgg
      481 acagcatgag gaagacgacg tgggggctcg cggggtagtc gatggcccgg acaatcttca
      541 tgggccacac ggtagtgggt gggcaagggc taccggccca ggccctggcg cacccgagcc
      601 gactggcgcg acagcgcgcc gtcgagatcg ctcacggtgc gcagcaggag cgcccgggcc
      661 cgggcgatgc gggcatggag ctggtcggcc tgctgctgca gggccgggac cgcctcccgt
      721 cgggccatgg cgtcatcgac cccagaaggc ccccagggct cactgacgcg ccactgctcg
      781 aggaccagtc ccccggcctc gacccgagcg cgccggaccc gtgctgcctc accgagctcg
      841 gcgagctcag tggcatagcg ctgcagggcc gcgccggctt cgtcaagaca ctcgatggtg
      901 cggtcgagca gcgccagctc gtccgcgaca cggctcgccg cccggccgtc ccgccggccc
      961 agcccggcca ggcccggcct cagggcgtcg cgaccgtcac cgagccgggc ggccgcgcgg
     1021 cgcaacgtcc ccccgagctc ggagcaggac gctgggtcgc catacggctc tggggtgctc
     1081 accgcagcca cggctccagc ggggtatggc gggccggagc cgccggtcgc cgtgggcctg
     1141 cggcgggctg ggtgccccat gcaggcccgt gggtgaccag ggcatcctca accgcatcgg
     1201 catgcgcccg ggcggtgtca ctcgcctgct cgacgaaggc gtcgagagct cgctgggcgg
     1261 tgtgctctcc gacgtccggg agcgcctgca gcagctcgtc ggcgacccgg agcagctcct
     1321 ggcgcagcac accgaggttg cgggcgaccc ccgtggcgcg cggcgagccc gccggtgccg
     1381 ctccgggatc catgtcttca cgatacgggg tccgagtccg gcagggccgg agttctccac
     1441 cggcgaggtc cgggcgggtg gcagcggcga gcgacgcgga gggataggct cggcagcggt
     1501 gcacaacggc gtgcgcccac ggatatgacg aaggacgcga cgtcgctcat gacgacttca
     1561 ctggagcagt cccgcgccca gctgatcagc accgcggccc atctgggaca gcggtccggc
     1621 gggccagacc tggaggcctt cctcgggcgc tactaccgac acgtggctac cgacgatctg
     1681 ctcgcccgag cacccgagga cctgctcggg gcggcgctgt cgcaccgcga gctcgcccgc
     1741 gaacgagccg tcggaagcgt caacgccgcg gcgttcaacc cctccgtcga cgcgcagggg
     1801 tggtcgagcg ggcacacggt ggtccaggtc gtctgcgacg acatgccgtt cctcgtcgac
     1861 tcggtcacct cagagctcgg ccgtgagagc cgcgccgtcc acctcgtcgt ccatccgctc
     1921 ctcgtggccc ggcgcaccat cgccggccac ctcgaggaga ttctcgacct cgacccccca
     1981 ggcccgcacg agttcggcac cggcgtcgag tcgtggatcc acgtcgagat cgaccgggcc
     2041 agctcggcgg ccgagctcga ggagatcgcg gcaaggctca ccgaggtgct cggcaacgtc
     2101 cgcgacgccg tcgaggactg gcccaagatg cgcgagcgcg tgcgcagcat cgccgacggg
     2161 ctgcgccatg gcggtccggc cggcataccc cagcgtgagg tcgacgagac cgtcgccttc
     2221 ctcgactgga tggtcgatga ccgcttcacc ttgctcggct accgcgagta cgccctggag
     2281 cgcgaggaag accacgacct gttgcgcacc cagccaagca gcgggctcgg tctcctgcgg
     2341 tatgacacca gccagtccgg ctcgttcgcc cggctcggcc ccctggtgca ggccaaggct
     2401 cgcgagaagc gcctgctcat catcaccaaa gccaacacgc gcgcgaccgt ccatcgctcg
     2461 gtctacctcg actacgtcgg ggtcaaggtc ttcgacgacg cgggggaggt cgtcggtgag
     2521 cagcggatcc tcgggctctt cgcgtccagc gcctacaacg cctcggtcct cgacatcccg
     2581 atcatggccg accgggtcaa cgccgtcctg cacctcgcgg ggttcacgca cgacagccac
     2641 tcgggcaagg acctgctcca ggtgctggag agctacccgc gtgacgagct gttccagacc
     2701 agcgtcgaga agctgctcga cgtcgcgtcg tccgtgctcc acgcccagga acggcgcaag
     2761 acccagctgt tcctgcgcaa ggacgactac ggccgtttca tgtcctgcct cgtcttcatc
     2821 ccacgcgccc gctacaacac gcccgtccgg ctcaagatcg agtcgcttct gcgcgaggcc
     2881 ttccacgcgg tcagcgtcga ctacacgacc cgggtgaccg agtcctcgct ggcccggcta
     2941 catttcgtcg tccgggtccg gcaaggtgag gtaatccccg acgtcgacga gccgggcctc
     3001 acccgcgcga tcgtcgaggc cgcccggacg tgggacgagg acctcgccga ggccgcccgg
     3061 accgagtatg gcgaggaggg cgcggcccgc ctcatggggc tgtactcccg ggcgttcccg
     3121 gaggcataca aggaggactt cggtccccgg gtcgcggtcg ccgacgtgcg tttcatcgag
     3181 tccctcaccg cgctggactc gaccgggctc aacatgtacc aggagcccgg cgcgccctca
     3241 cacgaacggc ggttcaagct cta
//
LOCUS       DATAIX010000230         6085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_184355, whole genome shotgun sequence.
ACCESSION   DATAIX010000230 DATAIX010000000
VERSION     DATAIX010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184355"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2928
                     /gene="uvrA"
                     /locus_tag="VHM65_03425"
     CDS             <1..2928
                     /gene="uvrA"
                     /locus_tag="VHM65_03425"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX2704784.1"
                     /translation="REHNLKDISVDLPRDSLIVFTGLSGSGKSSLAFDTIFAEGQRRY
                     VESLSAYARQFLGQMDKPDVDFIEGLSPAVSIDQKSTNRNPRSTVGTITEVYDYLRLL
                     FARAGRPHCPACGEPITRQSPQQIVDRLLELAEGTRFQVLAPVVRARKGEYVDLFAEL
                     QTKGFARARVDGEVVALTEPPALEKQKKHTIEVVVDRLVAKGPDAKAKRRLTDSVETA
                     LGLAGGTLLVDFVDVPAGAAEGDGGLPNERRYSERMACPNDHPLAMDEVEPRSFSFNS
                     PFGACTKCTGLGTELEVDADLCVPDPDLSLSQGAIAPWAQGSGMAEYFQRVIGGLATD
                     LKFSLDTPWAGLPERAKEALLFGENYRVHVTYRNRYGRDRSYTTGFEGVVPFIKRRHA
                     ETDSDWSRERYEGFMREVPCPQCRGARLKPESLAVLVGGQSISAVCALAIRECSEFLA
                     GVDFTDRERQIAERVIKEVQARLGFLLDVGLDYLSLDRPAGTLSGGEAQRIRLATQIG
                     SGLVGVLYVLDEPSIGLHQRDNRRLIETLTRLRDLGNTLIVVEHDEDTISHADWVVDI
                     GPGAGEHGGQVVHCGPVQGLLDHPDSITGKYLSGRLEIPTPAVRRPQDGREVRVLGAR
                     EHNLAGIDVTFPLGNLVAVTGVSGSGKSTLVNDILYNVLANKLNGARHVPGRHKSVAG
                     LEHLDKVVHVDQSPIGRTPRSNPATYTGVFDHMRRLFAETTEAKIRGYLPGRFSFNVK
                     GGRCDSCTGDGTIKIEMNFLPDVYVPCEVCHGARYNRETLEVHFKGRTIADVLDMPIE
                     EAADFFAAVPAIARHLRTLNDVGLGYVRLGQPAPTLSGGEAQRVKLASELQKRSTGRT
                     IYVLDEPTTGLHFEDIRKLLDVLQGLVDKGNTVIVIEHNLDVIKNADWVVDMGPEGGN
                     GGGRVVAEGTPERVAGVHASHTGRFLAPVLARTARTPTRGNAPAAGLKTTRPKVALRG
                     AAG"
     gene            3015..3482
                     /locus_tag="VHM65_03430"
     CDS             3015..3482
                     /locus_tag="VHM65_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX2704785.1"
                     /translation="MDQQNVTRRSVVQGAGLVGLGVVGAGALAACGDSSTPAAGDGAA
                     ASSTTSTAPGSSSSSAGGVSVKTADVPVGGGTIIATSLVVVTQPKAGEFKAFSAICTH
                     QKCPVTEIANGTIDCKCHGSKYDITTGAVVAGPAPSPLAAKTATVSGDTVTVS"
     gene            3540..5546
                     /gene="uvrC"
                     /locus_tag="VHM65_03435"
     CDS             3540..5546
                     /gene="uvrC"
                     /locus_tag="VHM65_03435"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX2704786.1"
                     /translation="MADPSTYRPRPGEIPVDPGVYRFRDSAGRVIYVGKAKSLRSRLG
                     SYFQDVAALHPRTATMVTTAAGVEWTVVATEVEALQLEYSWIKEFDPRFNVRYRDDKS
                     YPYLAVTLGEEFPRAQVMRGAKRKGTRYFGPYSHAWAIRETLDLVLRVFPIRTCSSGV
                     FKRAGQSGRPCLLGYIDKCAAPCVGRVDADEHRRIAEDFCDFMGGNTDQFVRRLERSM
                     RAAADELAFERAARLRDDLGALTKALERSAVVLGDGTDADIFALADDELEAAVQVFHV
                     RGGRIRGQRGWVVEKESEDLPLLVEHLLQQVYGESSPDGVPREVLVPVLPGEADVVMR
                     WLGERKGAKVDVRVPQRGDKRALMETVRRNAEQSLARHKVARAGDLTARSQALAELQE
                     GLGLDEAPLRIECFDVSHVQGTQVVASMVVFEDGLARRSEYRRFVVRGDAGVEGGAGD
                     EPAMVDDTRAMHEVLTRRFRRYLDDRAGATDIELGDGVPDAGPVSGPIDPATGKPRRF
                     AYPPNLVVVDGGLPQGNAAAAALGALGIDDVAVVGLAKRLEEVWCAGQDFPLILPRAS
                     EGLYLLQRVRDEAHRFAVTFHRQRRSTAMTASVLDAIPGLGPARRTALLAQLGSVKAI
                     RAASAEDLQAVPGVGPAMALAIVSHLATPATPAVNVTTGEVIDG"
     gene            5559..>6085
                     /locus_tag="VHM65_03440"
     CDS             5559..>6085
                     /locus_tag="VHM65_03440"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX2704787.1"
                     /translation="MSEVDSESRPMVILTGMSGAGRSTTANVLEDLGWYVIDNLPPQM
                     LGQLDELLSDVRRRAQPDVPPLAAVVDVRSRGWFVELSSEVRRLAGRGVRPTLIFLDA
                     TDEALVRRFESVRRPHPLQGEGRLLDGIVREREMLVGLRSDADMIIDTSGLNVHALTA
                     KITSIFAQPTAAQIR"
BASE COUNT          927 a         2211 c         2093 g          854 t
ORIGIN      
        1 cgcgagcaca acctcaagga catctcggtc gacctgcccc gcgacagcct catcgtcttc
       61 accgggctct cgggctcggg caagagctcg ctcgcgttcg acacgatctt cgccgagggg
      121 cagcgccggt atgtcgagtc gctgtcggcc tacgcccgcc agttcctcgg tcagatggac
      181 aaacccgacg tcgacttcat cgagggcctc tcaccggccg tgtcgatcga ccagaagtcg
      241 accaaccgca acccgcgctc gaccgtgggc acgatcaccg aggtctacga ctacctgcgc
      301 ctgctctttg cccgcgcggg tcggccgcac tgcccggcct gtggtgagcc gatcacccgc
      361 cagagcccgc agcagatcgt cgaccggctg ctggagctcg ccgaaggcac gcggttccag
      421 gtcctcgcgc cggtcgtgcg ggcgcgcaag ggcgagtacg tcgacctgtt cgccgagctg
      481 cagaccaagg ggttcgcccg cgcccgggtg gacggcgagg tcgtcgccct aaccgagccc
      541 ccggcgctgg agaagcagaa gaagcacacg atcgaggtcg tcgtcgaccg cctcgtcgcc
      601 aaggggccgg acgccaaggc caagcggcgg ctcaccgact cggtcgagac ggcgctaggg
      661 ctcgcgggcg ggacgctcct cgtcgacttc gtcgacgtgc cggccggcgc cgccgagggg
      721 gacgggggac tgccgaacga gcggcgctac tccgagcgta tggcctgccc caacgaccat
      781 cccctggcga tggacgaggt cgagcctcgc tcgttctcgt tcaactcgcc gttcggggcc
      841 tgcaccaagt gcaccggcct cgggaccgag ctcgaggtcg acgccgacct ctgcgttccc
      901 gacccggacc tgtcgctgtc gcagggagcg atcgccccat gggcccaggg gtcggggatg
      961 gccgagtact tccagcgcgt catcggcggg ctcgccaccg acctgaagtt ctcgctggac
     1021 acaccgtggg cgggactgcc ggagcgggcc aaggaggccc tgctcttcgg ggagaactac
     1081 cgggtccacg tgacctatcg caaccgctac ggccgggacc gctcctacac cacggggttc
     1141 gaaggcgtcg tcccgttcat caagcgccgg cacgccgaga ccgactcgga ctggagccgc
     1201 gagcggtacg aagggttcat gcgggaggtg ccgtgcccgc agtgccgggg cgcgcgcctc
     1261 aaacccgagt cgctcgcggt cctcgtcggc ggccagagca tctcggccgt ctgcgccctg
     1321 gcgatccggg agtgctccga gttcctcgcc ggcgtcgact tcaccgaccg cgagcgccag
     1381 atcgccgagc gggtcatcaa ggaggtccag gcccggctcg ggttcctcct cgacgtcggc
     1441 ctggactacc tctcgctcga ccgccccgcc gggaccctgt ccggcggaga ggcccagcgc
     1501 atccggctgg ccacccagat cgggtccggc ctggtcggcg tgctctacgt cctcgacgag
     1561 ccctcgatcg gcctgcacca gcgcgacaac cgccgtctca tcgagaccct gacccggctg
     1621 cgcgacctcg gcaacaccct catcgtcgtc gagcacgacg aggacacgat ctcccatgcc
     1681 gactgggtcg tcgacatcgg tccgggcgcc ggcgagcacg ggggacaggt cgtccactgc
     1741 ggcccggtcc aggggctgct cgaccacccc gactcgatca ccggcaagta cctctccggc
     1801 cgcctggaga tcccgacccc ggcggtgcgc cgcccccagg acggccgcga ggtccgggtc
     1861 ctcggtgccc gtgagcacaa cctcgccggc atcgacgtca ccttccccct cggcaacctc
     1921 gttgccgtca caggggtttc gggctcgggc aagtccaccc tggtcaacga catcctctac
     1981 aacgtcctgg ccaacaagct caacggcgcc cggcacgtcc ccggccggca caagtcggtg
     2041 gccgggctgg agcacctcga caaggtcgtc cacgtcgacc agagcccgat cgggcgcacc
     2101 ccgcggagca accccgcgac atacaccggg gtgttcgacc acatgcgccg gctcttcgcc
     2161 gagacgaccg aggccaagat ccgcggctac ctgccgggcc ggttctcgtt caacgtcaag
     2221 ggcggccgct gtgacagctg caccggcgac ggcacgatca agatcgagat gaacttcctg
     2281 cccgacgtct acgtgccgtg cgaggtctgc cacggggccc gctacaaccg cgagacgctc
     2341 gaggtccatt tcaagggcag gacgatcgcc gacgtcctcg acatgccgat cgaggaggcg
     2401 gccgacttct tcgctgccgt tcccgcgatc gcgcggcacc tgcggaccct caacgacgtc
     2461 ggcctcggct acgtccgcct cggccagccc gcgccgaccc tgtccggtgg cgaggcccag
     2521 cgggtcaagc tcgccagcga gctgcagaag cgctcgaccg ggcggacgat ctacgtcctc
     2581 gacgagccga cgacgggcct gcacttcgag gacatccgca agctgctcga cgtcctgcag
     2641 gggctcgtcg acaagggcaa cacggtcatc gtcatcgagc acaacctcga tgtcatcaag
     2701 aacgccgact gggtcgtcga catgggcccg gaaggcggca acggcggggg gcgggtcgtc
     2761 gccgagggaa cccccgagcg ggtcgcgggc gttcacgctt cgcacaccgg gcggttcctc
     2821 gcccccgtgc tggcccggac ggcgcgcacg cccacccggg ggaatgcccc ggcggcaggt
     2881 cttaagacca cccgcccgaa ggtcgcattg cggggcgccg caggctagtt gagactgcaa
     2941 tgacgagcgg gaacccagcg acatcgaccg gtatggcggg ccgcaggccc ccgccgcaga
     3001 acggaaggca gcacatggac cagcagaatg tgacccggcg cagcgtcgtt cagggcgcgg
     3061 gcctcgtcgg gctcggggtc gtcggcgcag gagcgctcgc cgcgtgcggc gactccagca
     3121 ccccggcggc gggagatggt gccgctgcgt cctcgacgac ctcaacggcc ccgggctcct
     3181 cgtcgagctc ggcggggggc gtctcggtca agacggccga cgtcccggtc gggggcggga
     3241 cgatcatcgc cacgtcgttg gtcgtcgtga cccagcccaa ggcgggggag ttcaaggcgt
     3301 tcagtgccat ctgcacgcac cagaagtgcc cggtcaccga gatcgcgaac ggcacgatcg
     3361 actgcaagtg ccacggcagc aagtacgaca tcacgaccgg ggcggtcgtg gccggcccgg
     3421 cgccgagccc gctcgcggcc aagacggcca ccgtctccgg agacaccgtc acggtctcct
     3481 gacccggcgg ccgagggcgg ccgaggggcg gcgtcatacc ggcctcgtag agttggcctg
     3541 tggctgaccc gtcgacctac cgaccacggc caggtgagat cccggtcgac cctggcgtgt
     3601 accggttccg ggactccgcc ggccgggtca tctacgtcgg caaggccaag tcgctgcgca
     3661 gccggctcgg gtcgtacttc caggacgtcg cggcgctgca cccccgcacc gcaacgatgg
     3721 tcaccacggc ggccggcgtc gagtggaccg tggtggcgac cgaggtcgag gccctgcagc
     3781 tcgagtactc ctggatcaag gagttcgacc cccggttcaa tgtgcgctac cgggacgaca
     3841 agtcctatcc ctacctcgcg gtgaccctgg gcgaggagtt tccccgcgcc caggtgatgc
     3901 gcggcgcgaa gcgcaagggg acccgctact tcgggcccta ctcgcacgca tgggcgatcc
     3961 gcgagacgct cgacctcgtg ctgcgagtct tcccgatacg tacctgcagc tccggggtct
     4021 tcaagcgggc cggtcagagc ggccggccgt gcctgctcgg ctacatcgac aagtgcgctg
     4081 ccccatgcgt cggccgggtc gatgccgacg agcaccgtcg catcgccgag gacttctgcg
     4141 acttcatggg aggcaacacc gaccagttcg tccggcggct cgagcggtcg atgcgcgctg
     4201 cggcggacga gctggcgttc gagcgggcgg cccggctccg cgacgacctc ggggcgctga
     4261 ccaaggcgct ggagcgctcc gccgtcgtcc tcggcgacgg gaccgacgcc gatatcttcg
     4321 ccctcgccga cgacgagctc gaggccgccg tgcaggtctt ccacgtccgc ggggggcgga
     4381 ttcgcggaca gcgcgggtgg gtcgtcgaga aggagagcga ggacctgccc ctgctcgtgg
     4441 agcacctgct ccagcaggtg tatggcgagt cctcgcctga cggtgtcccg cgcgaggtcc
     4501 tcgtgcccgt cctgccgggc gaggccgacg tcgtcatgcg gtggctcggc gagcgaaaag
     4561 gggcgaaggt cgacgtgcga gtcccgcagc ggggggacaa gcgcgcgctc atggagaccg
     4621 tccggcgcaa cgccgagcag tcgctggccc ggcacaaggt cgcccgggcc ggtgacctga
     4681 ccgcgcgcag ccaggccctg gccgagctcc aggaaggact cggcctggac gaggcgccgc
     4741 tgcgcatcga gtgtttcgac gtcagccatg tccagggcac ccaggtcgtc gcctcgatgg
     4801 tcgtcttcga ggacggactg gcgcgcaggt ccgagtaccg ccggttcgtc gtccggggcg
     4861 atgcgggcgt cgagggcggt gccggcgacg agcccgcgat ggtcgacgac acccgtgcca
     4921 tgcacgaggt gctgacccgt cgcttccgtc gctacctcga cgaccgggca ggggccaccg
     4981 atatcgagct gggagatggt gtcccggacg cgggcccggt cagcggaccg atcgaccccg
     5041 ccacgggcaa gccgcgccgg ttcgcctacc caccgaacct cgtcgtcgtc gacggtgggc
     5101 ttccccaggg caacgcggcg gccgccgcac tgggcgccct cggcatcgac gatgtcgcgg
     5161 tcgtcggcct ggccaaacgc ctcgaagagg tctggtgcgc cggccaggac ttcccgctca
     5221 tcctgccccg cgccagtgag gggctgtacc tcctccagcg ggtccgggac gaggctcacc
     5281 ggttcgccgt caccttccat cgccagcgcc ggtccacggc gatgaccgcc tcggtgctcg
     5341 acgccatacc cggcctggga ccggcccgca ggacggcgct gctcgcccag ctcgggtcgg
     5401 tcaaggcgat ccgggccgcc tcggccgagg acctgcaggc cgtcccgggc gtcgggcccg
     5461 ccatggccct ggcaattgtg agccacctgg cgacaccggc cacccctgcc gttaacgtga
     5521 cgaccggcga ggtcatcgat ggctagggag gacacgacat gagcgaggtg gacagcgagt
     5581 cacggcccat ggtcatcctc accgggatga gtggtgcagg ccggtcgacg accgccaacg
     5641 tgctggagga cctcggctgg tacgtcatcg acaacctccc accgcagatg ctcggccagc
     5701 tcgacgagct gctcagcgac gtgcggcgcc gggcgcagcc ggacgtccct ccgctggccg
     5761 ccgtcgtcga cgtccgaagc cgagggtggt tcgtcgagct gagcagcgag gtccggcgcc
     5821 tggccgggcg tggcgtgcgg cccacgctga tcttcctcga cgcgaccgac gaggcgctgg
     5881 tccgccggtt cgagagcgtc cgccgtcccc acccgctgca gggggagggc cgactgctcg
     5941 acggcatcgt ccgcgagcgc gagatgctcg tcgggctgcg ctcggacgcc gacatgatca
     6001 tagacacctc gggtctgaac gtccacgctc tcaccgccaa gatcacctcg atcttcgccc
     6061 agcccacggc cgctcagatc cgcat
//
LOCUS       DATAIX010000231         5394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_243847, whole genome shotgun sequence.
ACCESSION   DATAIX010000231 DATAIX010000000
VERSION     DATAIX010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243847"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..581)
                     /gene="coaD"
                     /locus_tag="VHM65_03445"
     CDS             complement(87..581)
                     /gene="coaD"
                     /locus_tag="VHM65_03445"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX2704788.1"
                     /translation="MTRRAVCPGSFDPVTNGHLDVATRAAALFDEVVVAVMHNPAKEG
                     TFPVEVRLEFLRTALQGQPTLRVEAFGGQLLVDVCRALDAVAIVKGLRDGADYAFELP
                     MAQMNRHLTGVETVFIPGNPAYAHVSSSLVKEVVRYGGDVSGLVPAAVLVALPAAVRG
                     GGIR"
     gene            635..1102
                     /gene="coaD"
                     /locus_tag="VHM65_03450"
                     /pseudo
     CDS             635..1102
                     /gene="coaD"
                     /locus_tag="VHM65_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
     gene            1295..1861
                     /locus_tag="VHM65_03455"
     CDS             1295..1861
                     /locus_tag="VHM65_03455"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEX2704789.1"
                     /translation="MFDTRELARRPGSMVELVRVAAAPDDFGTDIIAIPAGSPVELVL
                     RWESVVEGVLVSGSAHSTASGACVRCLDPVSLSVEARFQELFAYTDRAAHHRKVAGDG
                     AQADEADVYELVDDLFDLEPVLRDAVVPTLPFQPVCRADCPGLCSECGARLADDPDHR
                     HDVTDPRWAALQALSSPAPADPTEERRT"
     gene            1863..2057
                     /gene="rpmF"
                     /locus_tag="VHM65_03460"
     CDS             1863..2057
                     /gene="rpmF"
                     /locus_tag="VHM65_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX2704790.1"
                     /translation="MAVPKRKTSRSNTRSRRANWKATAPTLTACPQCKEPKAPHLACP
                     SCGMYNGRHYGEAERTEHQA"
     gene            2072..2860
                     /gene="rnc"
                     /locus_tag="VHM65_03465"
     CDS             2072..2860
                     /gene="rnc"
                     /locus_tag="VHM65_03465"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX2704791.1"
                     /translation="MTRLPAVPSGPRLPAAALHGDLTEVLGAPLDEQLLTRALTHRSY
                     AYEHGGLPNNERLEFLGDAVLGLVVTETLYAIHPDLPEGQLAKLRAAVVNMRALAEVS
                     RTVGLGEHLLLGRGEESTGGRNKDSILADTLEAVIGTVFLCAGMPGARTFVHHLFDPL
                     IATSAVRGAGLDWKTSLQEITSAGGFGAPDYKLEESGPDHEKSFTAVVHIDGGALGEG
                     EGRTKKEAEQHAAEQAWTALDERRSAHCPPPSADSADGPVGADA"
     gene            2853..3737
                     /gene="mutM"
                     /locus_tag="VHM65_03470"
     CDS             2853..3737
                     /gene="mutM"
                     /locus_tag="VHM65_03470"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX2704792.1"
                     /translation="MPELPEVEMVRRGLAEHVVGRQIRQATILGTRVARRHPGGPADL
                     AVRLTGATVRAAHRRGKYLWLDLDAPGQQGQALVVHLGMSGQLLVEPEDAPRERHLHA
                     SLDFADGGPQLRFVDQRTFGGLLLADVDGDGIPDPVRHIGADPFDPRFDQAGVVRRLR
                     ARHTGIKRALLDQTLVSGIGNIYADEALWRGQVHGEREAAALSKPTLARVLEHARAVI
                     GAALEVGGTSFDALYVNVNGASGYFDRSLDAYGQEGRPCRRCGTAIRREAFMNRSSYS
                     CPRCQPRPRLAGRVSTGR"
     gene            3748..>5394
                     /locus_tag="VHM65_03475"
     CDS             3748..>5394
                     /locus_tag="VHM65_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX2704793.1"
                     /translation="MSTGLRSPVDLPSAGAAAGWLDARVTGELDRARRLVEELKATPP
                     EGALAVLRSWNDIALAMSNVGALGSLFSEVHPDEAVRTRAESAIQDLQRLDTELGLDA
                     GLFAVFSALDPAGLDALATRLLAKTVLDFRRAGVDRDEATRERIRTLQERAVLVGQEF
                     SKAIRDDVRSIRVAPERLAGMPQDWLDAHPVGEDGLVTVTTDYPDSIPLRTFCRDAAA
                     RREMAVAFLSIGWPRNDARLRELFAIRAELAQLLGYRDWADFDAQVKMIGRGEAIPPF
                     IDKMAAASRESGMRDRQVVLDRLRQDVPGATTIDGADLMYYAEAVRREQHDVDAQEVR
                     RYFDFPRVRQGLLDVTGRLFGLRYQPVAEAVVWDPDVVVYDVHLGRDRGGEHIGRIYL
                     DLHPRGGKYKHAAQFDLGRGVAGRQLAEGVLACNFPRGLMEHDQVVTLFHEFGHLVHH
                     VLAGRGEWVRFSGVATEWDFVEAPSQMLEEWAWDAQVLRTFAVDESGEPIPAELVARM
                     RDADHFGKGYQARTQMFYAAMSYYFHVEQPEDITARCRELQ"
BASE COUNT          779 a         1964 c         1894 g          757 t
ORIGIN      
        1 atgtcagggc agacacagac cgacgcacca gagtaccgga gtgcccgatc cccccataat
       61 cccggacggc ctcttcccgg acaccctcag cgtatgccgc cgccgcgcac cgccgcgggc
      121 agggccacca ggacggccgc aggcacgagc ccggagacgt cgccgccata ccggacgacc
      181 tccttgacca gggagctcga gacgtgggcg taggccgggt tgccggggat gaagacggtc
      241 tcgaccccgg taaggtgacg gttcatctgg gccatcggca gctcgaaggc gtagtcggcg
      301 ccgtcccgca gccccttgac gatcgcgacg gcatcgagcg cgcggcagac atcgacgagg
      361 agctggcccc cgaacgcctc gacgcggagc gtgggctgtc cctgcagcgc ggtccgcaag
      421 aactccagac gcacctcgac ggggaaggtc ccctctttgg cggggttgtg catcaccgcg
      481 acgacgacct cgtcgaagag cgcggccgcc cgggtcgcga catcgaggtg gccgttcgtc
      541 acagggtcga acgagccggg gcagaccgca cgcctggtca cagcgccgac cgtagcagga
      601 ccggcggtgc ggtggtcagg cgacctcgcc cggctcgttc gaccctgtga cgaacggcca
      661 cctcgacgtc gcgacccggg cggccgcgct cttcgacgag gtggtcgtcg cggtgatgca
      721 caaccccgcc aaggagggca cctttcccgt cgacgtgcgt ctggagttcc tgcggacggc
      781 gctgcagggg cagcccaccg tccgcgtcga ggcgttcgtg ggccagctcc tcgtcgacgt
      841 ctgccgcagg ctcgatgccg tcgcgatcgt caaggggctc cgggacggcg ccgactacgc
      901 cttcgagctg ccgatggccc agatgaaccg ccacctcacc ggggtcgaga ccgttttcgt
      961 ccccggcaac ccggtgtacg cccacgtctc gagctccctg gtcaaggagg tcgtccggta
     1021 tggcggcgac gtctccggac tcgtgcctgc ggccgtcctg gcggccctgc ccgcggcggt
     1081 gcgcggcggc ggcatacgct gagggtgtcc gggaagaggc cgtccgggat tatgggggga
     1141 tcgggcactc cggtactctg gtgcgtcggt ctgtgtctgc cctgacatgg cccgtatggc
     1201 gtgacacggc tgccgtgaca cgcctgccgt gacacgaacc aagccaacac gactgtggga
     1261 tgtgatgaac cgcctcgacc ctcgagcacc gttggtgttc gacacgcgtg agcttgctcg
     1321 ccggccaggg tcgatggtgg agctcgtccg tgtcgctgcg gcgcccgatg acttcgggac
     1381 cgatatcatc gccatcccgg cgggttcgcc cgtcgagctg gtccttcggt gggagtccgt
     1441 cgtcgaaggc gtcctggtca gcggctcggc ccacagcacg gccagcggtg cctgcgtgcg
     1501 gtgcctggac ccggtgtccc tctctgtcga ggcccggttt caggagctgt tcgcctacac
     1561 cgaccgtgcg gcccaccacc gcaaggtggc gggggacggc gcccaggccg acgaggccga
     1621 cgtgtacgag ctcgtggacg acctgttcga cctcgagccc gtgctccggg acgcagtggt
     1681 gcccaccctg ccgttccagc cggtatgccg cgccgactgt ccgggtctgt gctccgaatg
     1741 cggagcgcgc ctggcggacg acccggacca ccggcacgac gtgaccgacc cgagatgggc
     1801 ggcgttgcag gcgctttcga gcccggcacc ggccgacccg accgaagaga gaaggacctg
     1861 acgtggctgt cccgaaacgc aagacgtcgc gctcgaacac ccgctcgcgc cgtgcgaact
     1921 ggaaggcgac cgcgcccacg ctcaccgcgt gcccgcagtg caaggagccc aaggctccgc
     1981 acctggcctg cccgtcgtgc ggcatgtaca acggccgtca ctacggggag gccgagcgga
     2041 ccgagcacca ggcctgaccc cggtcctcct ggtgacccgg ctccctgccg ttccctccgg
     2101 accccggctg cccgccgcgg cgctgcacgg ggacctcacc gaggtgctcg gcgcgcccct
     2161 cgacgaacag ctgctcaccc gtgccctgac gcaccggtcg tacgcctacg agcacggagg
     2221 gctgcccaac aacgagcggc tcgagttcct cggggatgcc gtgctcgggc tcgtcgtcac
     2281 cgagacgctc tatgcgatcc accccgacct gcccgaaggg cagctcgcca agctgcgggc
     2341 ggccgtcgtc aacatgcggg cgctggccga ggtgagccgg acggtgggcc tcggtgagca
     2401 cctgctgctc ggccgcggcg aggagtcaac cggcgggcgc aacaaggact cgatcctcgc
     2461 cgacaccctc gaagcggtca tcgggacggt gtttctctgc gccggtatgc cgggggcacg
     2521 cacgttcgtc catcacctgt tcgacccgct catcgcgacc tcggcggtgc gcggagccgg
     2581 tctggactgg aagacaagcc tgcaggagat cacctccgcc ggtggcttcg gggccccgga
     2641 ctacaagctc gaggagtccg gtccggacca cgagaagtcc ttcaccgcag tcgtgcacat
     2701 cgacggtggg gcgctcggcg agggcgaggg ccggaccaag aaggaagccg agcagcacgc
     2761 cgccgagcag gcgtggacgg ccctggacga gcgccggagc gcgcactgcc cgccaccttc
     2821 ggccgactcg gccgacggcc ccgtaggagc cgatgcctga gctgcccgag gtcgagatgg
     2881 tccggcgcgg cctcgccgag cacgtcgtcg gacgccagat ccgccaggcc acgatcctcg
     2941 gcacccgggt ggcccgccgt caccccggcg gccccgcgga cctggccgtc cggctcaccg
     3001 gcgcgaccgt gcgggcagcg caccgccggg gcaagtacct ctggttggac ctcgacgcac
     3061 ccggtcagca gggtcaggcg ctcgtcgtcc atctcggcat gagcggccag ctgctcgtcg
     3121 agccggagga cgccccgcgc gagaggcatc tgcacgcgtc gctggacttc gccgacggcg
     3181 ggccccagct gcgcttcgtg gaccagcgca cgttcggcgg gctcctgctc gcggacgtcg
     3241 acggcgacgg cataccggac ccggtccggc acatcggcgc ggacccgttc gacccgcgct
     3301 tcgaccaggc aggcgtcgtg cgcaggctca gggcccgcca taccggcatc aaacgagcgc
     3361 tcctggacca gacgctcgtc tcggggatcg gcaacatcta tgccgacgag gcgctgtggc
     3421 gggggcaggt ccacggcgag cgcgaagccg cggcgctgtc caaacccacg ctggctcggg
     3481 tgctcgagca cgcccgtgcg gtgatcgggg ccgcgctcga ggtgggcggc acgagcttcg
     3541 atgcgctcta tgtcaacgtc aacggggcct cggggtactt cgaccggtcc ctcgacgcct
     3601 acggccagga ggggcgcccg tgccgccggt gcggcacggc gatccgccgg gaggcgttca
     3661 tgaaccgctc gtcctacagc tgcccgcggt gccagccacg gccccgcctc gcgggtcgtg
     3721 tcagcaccgg ccgctaaagt gccgcgagtg agcaccggac tgcgcagccc cgtcgacctc
     3781 ccctccgccg gcgccgctgc cgggtggctc gacgcgcggg tcacgggcga gctcgaccgg
     3841 gcccgccgcc tcgtcgagga gctcaaggcc actccgccgg agggtgcgct cgcggtactg
     3901 cgctcgtgga acgacatcgc gctggccatg agcaacgtcg gcgcgctcgg gtcgctgttc
     3961 agcgaggtgc acccggacga ggcggtgcgg acccgcgccg agagcgccat ccaggacctg
     4021 cagcggctcg acaccgagct gggcctggac gccggcctgt tcgccgtctt ctcggccctc
     4081 gacccggccg ggctcgacgc cttggccacc cggctgctcg ccaagaccgt gctcgacttc
     4141 cgccgcgccg gtgtcgaccg cgacgaggcg acccgcgagc ggatccgaac cctgcaggag
     4201 cgtgccgtcc tggtcggcca ggagttcagc aaggcaatcc gtgacgacgt ccggagcatc
     4261 cgcgtggcgc ccgagcggct ggccgggatg ccccaggact ggctcgacgc ccatccggtc
     4321 ggggaggacg ggctcgtcac ggtgacgacc gactaccccg actcgatccc cctgcggacc
     4381 ttctgccgcg acgcggcggc gcgccgggag atggccgtcg cgttcctctc gatcggctgg
     4441 ccgcgcaacg acgcccggct cagggagctc ttcgcgatcc gggccgagct ggcgcagctg
     4501 ctcggctatc gcgactgggc cgacttcgac gcccaggtca agatgatcgg gcggggcgag
     4561 gccatacccc ccttcatcga caagatggcc gccgcctcgc gggagtcggg aatgcgcgac
     4621 cgccaggtcg tcctcgaccg gctccgtcag gacgtgcccg gcgcgacgac catcgacggc
     4681 gcggacctca tgtactacgc cgaggcggtg cgccgcgagc agcacgacgt cgacgcccag
     4741 gaggtgcgcc gctacttcga cttcccccgg gtgcgtcagg ggctgctcga cgtcaccggc
     4801 cggctgttcg gcctgcgcta ccagccggtc gccgaagccg tcgtctggga ccccgatgtc
     4861 gtcgtgtacg acgtccacct cggccgtgac cgcgggggcg agcacatcgg gcggatctat
     4921 ctcgaccttc acccgcgcgg gggcaagtac aagcacgccg cgcagttcga cctcggtcgt
     4981 ggggtcgccg ggcgccagct cgctgagggc gtcctggcct gcaacttccc ccgcgggctg
     5041 atggagcacg accaggtcgt cacgctcttc cacgagttcg gccacctcgt ccatcacgtg
     5101 ctggcgggcc ggggggagtg ggtgcggttc tccggcgtcg ccaccgagtg ggacttcgtc
     5161 gaggcaccga gccagatgct cgaggagtgg gcctgggatg cccaggtcct gcggaccttc
     5221 gcggtcgacg agtccggcga gccgatcccg gccgagctgg tcgcccggat gcgcgacgcc
     5281 gaccacttcg gcaagggcta ccaggcgcgc acccagatgt tctacgccgc gatgtcctac
     5341 tacttccacg tcgagcagcc cgaggacatc acggcccgat gccgcgagct ccag
//
LOCUS       DATAIX010000232         3825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_161960, whole genome shotgun sequence.
ACCESSION   DATAIX010000232 DATAIX010000000
VERSION     DATAIX010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161960"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VHM65_03480"
     CDS             complement(<1..884)
                     /locus_tag="VHM65_03480"
                     /inference="COORDINATES: protein motif:HMM:NF023702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HEX2704794.1"
                     /translation="MATLTEVLSTHPRLSPEDVEWLTLLVGDWQLLADLSFADLVLWV
                     SSDPGWAVVAHVRPTTGQTLFFDDQVGHLASAGRAAVLSRAEADHRIVRGRDPEWGAD
                     TPIREESIPVIRRGRVLAILTRHTNLAAMRTPSRLELTYLSTADALVRMIASGDFPHG
                     AAPTGMRRGAPRVGDGVLRLDAQGQIRYASPNAVSALHRLGHVGDLAGHSLARIVTDV
                     LRDPSPVDEGLPFVLTGRQAWRTEVCSGGATVTMRAIPLTEDGVRVGAVLLLRDVSEL
                     RRREQELLTKDATIREIH"
     gene            complement(927..2168)
                     /locus_tag="VHM65_03485"
     CDS             complement(927..2168)
                     /locus_tag="VHM65_03485"
                     /inference="COORDINATES: protein motif:HMM:NF044969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704795.1"
                     /translation="MTDEVAEKVLRDNYEQNVLLGNARAQQHMMLPVHTRLIHQLEER
                     GILDRALEFMPSDAELAKRNDTGHGLTSPELCVLVAFTKIALKDDLLPSALPDDPWFQ
                     STLLDYFPEQIRMHHADDLAAHPLRREIVTNAVVNSMVNRGGITFAYRAVEETGATVE
                     QVSRAFVVCREVFNLAGFVRQVEALDNHVSTSTQTALYLEFRRLLDRAVRWFLTNRPT
                     SLDVAAEVDRFSSTVAKLGPQIPELLQGGEHKRLERRAREMEKAGVPAEFALNVAALL
                     DQFSLLDIVELATETLVPAAEVAAIYYVTSERFGIDEMLTRVTRLPRDDRWDALARGA
                     LRDDLYAVLETLTHAVIEASSDGQTPAKRFDAWASANAESLRRARAALGGIERLEQPG
                     IAPLSVALRTLRAVSQSGSSG"
     gene            complement(2166..3797)
                     /locus_tag="VHM65_03490"
                     /pseudo
     CDS             complement(2166..3797)
                     /locus_tag="VHM65_03490"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
BASE COUNT          580 a         1288 c         1378 g          579 t
ORIGIN      
        1 tggtggatct cccggatcgt cgcgtccttg gtgagcagct cctgctcccg ccggcgcagc
       61 tcgctgacat cgcgaagcag caggaccgcg ccgacccgga cgccgtcctc ggtgagcggt
      121 atggcgcgca tcgtcaccgt cgcgccgccc gagcagacct cggtgcgcca ggcctgacgg
      181 ccggtgagga cgaagggcag gccttcgtcg accggcgatg ggtcgcggag cacgtcggtg
      241 acgatccggg ccagcgagtg cccggccagg tcaccgacat gcccgagacg gtgcagcgcc
      301 gacactgcgt tggggctggc atacctgatc tggccctggg catcgaggcg cagcacgcca
      361 tcaccgaccc ggggggcgcc acggcgcatc cccgtcggcg ccgccccatg cgggaagtcc
      421 cccgaggcga tcatccggac gagtgcgtcg gccgtcgaca ggtacgtgag ctcgagccgg
      481 ctcggggtgc gcatcgcggc aaggttggta tggcgggtca ggatcgccag gacccggcca
      541 cgccggatga ccgggatcga ctcctcacgg atcggcgtat cggcgcccca ctccgggtct
      601 cggccacgga cgatccgatg atcggcctcg gcacggctga gcacagcggc ccgtccggcg
      661 cttgccagat gcccgacctg gtcgtcgaag aagagtgtct gacccgtcgt gggtcgcacg
      721 tgcgcgacga cggcccagcc tggatcggag ctgacccaga gcacgaggtc ggcgaaggac
      781 agatcggcga gcagctgcca gtcccccacg agcagcgtga gccattcgac gtcctccggc
      841 gagaggcggg gatgcgtcga cagcacctcg gtcagggtgg ccacgatccg acagcgtagc
      901 cgcgtcgcag gcccgcgggc cgaccgctat ccggaggagc cgctctggct caccgcacgc
      961 agcgtccgca gggccacgga caacggagcg atcccgggct gctcgaggcg ctcgatgcca
     1021 cccagtgcgg cccgcgcacg gcgcagcgac tcggcgttgg cgctcgccca ggcgtcgaag
     1081 cgcttggccg gggtctgacc gtccgagctg gcctcgatga ccgcgtgggt cagcgtctcg
     1141 agtacggcgt agaggtcgtc gcgcagggca ccgcgcgcca aggcatccca ccggtcgtcg
     1201 cgcgggagcc gggtcacccg ggtcagcatc tcgtcgatgc cgaaccgttc cgacgtcacg
     1261 tagtagatcg cggcgacctc agccgccggc acgagggtct cggtcgccag ctcgacgatg
     1321 tcgagcaggc tgaactggtc gagcaacgcg gcgacattga gcgcgaactc ggctggcacc
     1381 ccggccttct ccatctcgcg agcgcgccgc tccagtcgct tgtgctcgcc gccctgaagg
     1441 agctccggga tctgcggccc gagcttggcc accgtggagg agaagcggtc gacctcggcc
     1501 gcgacgtcga ggctcgtcgg acggttggtg aggaaccacc gcaccgcacg atcgagcagc
     1561 cgccggaact cgaggtatag cgccgtctgg gtgctcgtcg agacgtggtt gtccagtgcc
     1621 tcgacctggc ggacgaaccc ggccaggttg aagacctccc ggcacacgac gaacgcccgg
     1681 gacacctgct ccaccgtggc cccggtctcc tcgacggcac ggtaggcgaa ggtgatgccg
     1741 ccccggttga ccatcgagtt gacgaccgcg ttggtgacga tctcgcgccg cagcgggtgc
     1801 gcggcgaggt cgtcggcatg gtgcatcctg atctgctcgg ggaagtagtc gagcagcgtg
     1861 ctctggaacc acgggtcgtc cggcagcgcg gacgggagca ggtcgtcctt gagcgcgatc
     1921 ttggtgaacg cgacgaggac gcagagctcc ggggaggtca gcccatggcc ggtgtcgttg
     1981 cgcttggcca gctcggcatc gctcggcatg aactccaggg cgcggtcgag gatgccccgc
     2041 tcctcgagct ggtggatgag ccgcgtgtgg accggcagca tcatgtgctg ctgggcccgc
     2101 gcgttgccga gcaggacatt ctgctcgtag ttgtcgcgca ggaccttctc ggccacctcg
     2161 tcggtcatcg agacgagcag cttgttgcgc tgcttcaggg tcatgtcccc gtcgcgcatg
     2221 aggccggtca gcaagatctt gatgttgacc tcgtggtcgg aggtgtcgac cccggccgag
     2281 ttgtcgatgg cgtcggtgtt gacacggacg ccgcgcaggc tcgcctcgac ccgcccgagc
     2341 tggctgcagc ccaggttgcc gccttccccg atcaccttga ctcgcagccg gctcccgtcg
     2401 acgcggatcg cgtcgttggc ccggtcgccg atgtcggcgt cgctctccgt ggacgccttg
     2461 aggtaggtgc cgatgccacc gttccagagc aggtcgaccg gcgcgagcag gatcgcgcgc
     2521 atgagctcgg caggggtgag cttggcgacc gtgtcagcca ggccgagtgc agccgcggcc
     2581 tgcgggctga ccggcaccga cttggcactg cggtcgaaga cgccaccgcc ctcgctgatg
     2641 agctcgcggc ggtagtcccg ccatgaggac cgtggcaggt cgagcagcct gcgacgttcg
     2701 gcgaacgact ctgccgcgtc ggggctgggg tcgatgaaga tgtggcggtg gtcgaacgcg
     2761 gccacgagac ggatgtgctc ggagcagatc atcgcgttgc cgaacacgtc gccgctcatg
     2821 tcccccactc cgacgcaggt gaagtcctgc gtctgggtgt cgacccccag ctcacggaag
     2881 tgccgcttga ccgactccca ggcgccgcgg gccgtgatcc ccatggcctt gtggtcatac
     2941 ccggccgacc cccccgacgc gaacgcgtcg tcgagccaga agcccgcctc ctgggcgacg
     3001 ccgttggcga tgtcggagaa ggtcgccgtg cccttgtcgg ccgcgacgac gaggtaggag
     3061 tcgtcctggt catgacgaac gacgtcgacc ggcgcgatga tccgcccgct gacccggttg
     3121 tcggtgagat cgagcagacc ggagatgagc agcttgtatg acgcgatccc ctcggccagc
     3181 cacgcctcgc ggtcgacgga ggggtcaggc aggttcttgg cgaagaagcc gcccttggaa
     3241 ccggtcggga cgatgaccga gttcttgacc atctgggcct tgaccagacc caggacctcg
     3301 gtgcggaagt cctcgcggcg gtccgaccag cgcagcccgc accgggcgac cttgccggag
     3361 cgcaggtgca cgccctagac ccgggggctg tagacccaga gctcgtaggc cggccgtggc
     3421 gcgggcaggt cgggcacctc cttggggttc agcttgaggc tgacgtagga cttggccgcg
     3481 cccgcggcat cgagctggaa gtagttcgtg cgcagcccgg cggtgacgac gccgagcagc
     3541 gcccggatga tccggtcgtg gtcgaggctc ttgaccgcgt caaggtcgct catgagcgag
     3601 gcgacgatcg atgcctgcgc ggccgaacgg ccggccgggt cctggccgaa ccgggcaggg
     3661 tcgaaccggg tctcgaacag gacaacgagc cggcgggcaa tcgtggggtt ggaggtcagt
     3721 gccgcctcga catagtcctg ggagacccgg gactgcgtct ggcgcaggta cttggccacc
     3781 gcgcgcagca cgaccacctg gcgccaggtg gtcgtgctgc gcgcg
//
LOCUS       DATAIX010000233         3312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_213990, whole genome shotgun sequence.
ACCESSION   DATAIX010000233 DATAIX010000000
VERSION     DATAIX010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213990"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..387)
                     /locus_tag="VHM65_03495"
     CDS             complement(160..387)
                     /locus_tag="VHM65_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704796.1"
                     /translation="MDDRVEVLGADRRRHDEAHAFVCRWHVRRVVRATVHGDINPTID
                     EPFAQLLDVVLDAAKGGRQSLLPDHRDVYAN"
     gene            complement(506..1597)
                     /locus_tag="VHM65_03500"
     CDS             complement(506..1597)
                     /locus_tag="VHM65_03500"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX2704797.1"
                     /translation="MDIDWFLTAACFGIGIVVGLTGMGGGALMTPVLVLFFNIPPLTA
                     VSSDLAAAAVMKPVGSFVHLRHGTVNLSLVKWLCLGSIPAAFSGVFIIRALGNSESIE
                     ELTKTALGFALLIAATGLTIRAYMRLVQRARKRDGHLPPEPKGRPDVVVRVIPTIILG
                     VIGGVMVGMTSVGSGSLIIIALMALCPTLRASELVGTDLAQAVPLVASAALAHVFYGD
                     FQLSLTTSLLIGSLPGVWIGAHLSSRAPGGIVRRALAFVLLASSFKLLGLSNQQAGLL
                     LGVLLPTWLQPELVAQPVRERAGLRRRDPVGDVARRAPELASVVLGEGLADRDDLVGH
                     VAAHPEGRSQARPEPRRPTWPVGGRVRAP"
     gene            complement(1597..3027)
                     /gene="cysC"
                     /locus_tag="VHM65_03505"
     CDS             complement(1597..3027)
                     /gene="cysC"
                     /locus_tag="VHM65_03505"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX2704798.1"
                     /translation="MSERTVPTPMPTHVLAPAELRDVELALFGALGDGHVLGGPVTAG
                     ADPTAATQSSRVLLALPVDPRARTLAHGAVQLLDDELTPIARLTAVTDVRDATGTSDG
                     LVSGTLVRERWRESGSARDHALTDSDVTGLHADAVLVLGRPPVDADAEALAGLTAQTL
                     TGPQLSPRTLLVAVPDRPEDTQGVPTGAMLSVATGLVARLGRQAEAQLRVIPLARRDK
                     ASDRALAHAFAARVGARAVVLLGVGERTPADTTWQGALATLQRGTDLARIPALAADDE
                     RLLRRWRRPRRERGLVLMFSGLSGSGKSTLARGAGTWLERHTDRTVSLLDGDVVRRML
                     SSGLGFDRESRILNVRRIGYVGAEVARHGGVAICAPIAPYASTRAEVRAMAEAVGDFV
                     LVHVSTPIEECERRDLKGLYAKARAGLIPEFTGVSDPYDVPADAEVVVDTSALSPADA
                     VRTVADFLRAGGWVTDTDPTSSQGTP"
BASE COUNT          527 a         1111 c         1248 g          426 t
ORIGIN      
        1 gcgcacgacg gtgccgctca tggcggacga atactcgcgc aaccgtacga cgggggggtt
       61 catcctcgtc gacgagggaa ccaaccgcac cgtcgcggcc ggaatgatca ccgaagcgag
      121 ctgaggctat ggctggcccg cccgcgcggg cgtcggcggt tagttagcat acacgtcgcg
      181 atgatcgggc agaagggact gcctgccacc tttggcggca tcgagcacca cgtcgagcag
      241 ctgggcgaac ggctcgtcga tcgtggggtt gatgtcaccg tgtactgtcg cgcgaactac
      301 gcggcggaca tgccatcgac atacaaaggc atgcgcctcg tcgtgacgcc gacgatcggc
      361 accaagcacc tcgacgcgat cgtccacagc gcgacgtcga cagcgcacgc gatgaggtcc
      421 ggtgtcggca tcgtgcacta ccacgcgatc ggtccgggtc tggtggctcc gctgccgcga
      481 ttcgcctccc gggccaaggt tgccctcacg gtgcacggac tcgaccacca acgggccaag
      541 tggggcggcg tggctcaggc cgtgcttggg aacgtccctc tggatgagcg gccacgtgcc
      601 caacgagatc atcacggtca gccagtccct cgccgagcac taccgacgcg agttcgggcg
      661 cacggcgagc tacatcccca acggggtcgc gacgccgcag cccggcccgc tctcggaccg
      721 gttgcgcaac cagttcgggc tggagccagg tcggtagcag cacaccgaga aggagcccgg
      781 cctgctggtt ggagagcccg agcagcttga aggaggaggc gagcaggacg aaggcgagcg
      841 cccggcggac gatgccaccg ggtgcccgtg aggacaggtg ggctccgatc cacacgccgg
      901 ggaggctccc gatgagcagg gatgtggtaa gcgagagctg gaagtcgccg tagaagacgt
      961 gcgccagggc cgccgaggcg acgagcggca cggcctgggc gaggtcggtc ccgacgagct
     1021 cactggcccg aagggtgggg cagagcgcca tgagggcgat gatgatgagc gaccccgacc
     1081 cgacggaggt catgccgacc atgacaccac cgatgacgcc gaggataatc gtcgggatca
     1141 ccctcacgac gacgtcaggg cgtcccttgg gttcgggagg caggtggccg tcgcgcttgc
     1201 gcgcccgctg gacgaggcgc atgtaggcgc ggatggtcag gccggtcgcc gcgatgagga
     1261 gggcaaagcc cagcgcggtc ttggtcagct cctcgatcga ctccgagttg cccagcgcgc
     1321 ggatgatgaa cacgcccgag aacgccgccg ggatcgagcc gaggcagagc catttgacga
     1381 ggctgaggtt gaccgtgccg tggcggaggt ggacgaagga ccccaccggt ttcatcaccg
     1441 cggcggcggc gaggtcgctg gagacggcgg tgaggggcgg gatgttgaag aacagcacga
     1501 ggacgggcgt catcagcgcc cctccaccca tcccggtgag cccgacgacg atgccgatac
     1561 cgaagcacgc ggcggtcagg aaccagtcga tgtccatcag ggtgtccctt gtgaagaggt
     1621 cgggtcggtg tcggtgaccc agccaccggc ccgcaggaag tccgcaaccg tgcgcacggc
     1681 atcggccggg ctcagggccg aggtgtcgac gacgacctcg gcgtcggccg ggacgtcata
     1741 cgggtcgctc actccggtga actcggggat cagaccggcc cgggctttgg cgtagaggcc
     1801 cttgaggtcg cggcgctcgc actcctcgat cggggtgctg acgtgcacga ggacgaagtc
     1861 cccgacggcc tcggccatgg cgcgcacctc ggcccgggtc gaggcgtagg gtgcgatcgg
     1921 cgcgcagatc gcgacgccgc cgtggcgcgc gacctcggcg ccgacatacc cgatcctgcg
     1981 cacgttgagg atgcgtgact cgcggtcgaa acccagcccc gaggacagca tccggcggac
     2041 gacgtcgccg tcgagcaggg agaccgtccg gtcggtatga cgttccagcc acgtcccggc
     2101 cccccgggcg agggtggact tgcctgagcc cgaaagcccg ctgaacatca gcacgagccc
     2161 gcgttcgcgc cggggtctgc gccaccgcct gagcagccgc tcgtcgtcgg cggcgagggc
     2221 cgggatccgg gcgaggtcgg tgccgcgctg gagggtggcc agagccccct gccaggtcgt
     2281 gtcggcgggc gtccgttccc ctaccccgag caggacgacc gcacgcgcgc cgacacgggc
     2341 ggcgaaggca tgcgcgaggg cgcggtcgct ggccttgtcc cggcgagcga gcggtatgac
     2401 gcgcagctgc gcctcggcct gtcgaccgag gcgggccacg agcccggtgg cgacggagag
     2461 catcgccccg gtcggcacgc cctgggtgtc ctcaggccgg tccggaacgg ccacgagcag
     2521 ggtccgcggc gagagctgcg ggccggtgag cgtctgggcg gtgagaccgg ccagggcctc
     2581 ggcatcggca tcgaccggag ggcggcccag gacgagcacg gcatcggcgt gcagcccggt
     2641 gacgtcggag tcggtcaagg catggtcgcg ggcggagccg ctctcacgcc agcgctcgcg
     2701 cacgagggtg ccgctgacca ggccatccga ggtcccggtc gcgtcgcgca catcggtgac
     2761 ggcggtcagc cgggcgatcg gggtgagctc gtcgtcgagg agctggacgg cgccatgagc
     2821 gagcgtccgg gcccgcgggt cgacggggag cgcgaggagc acccgggacg actgggtcgc
     2881 cgcggtgggg tctgcgccgg ccgtgaccgg gccaccgagg acgtgcccgt cgccgagcgc
     2941 cccgaacagc gcgagctcga cgtcgcgcag ctccgccggc gccagcacgt gggtcggcat
     3001 cggcgtgggc acggtgcgct cggacatggg ctccccctcg gcatcggtgg atatccccgg
     3061 acgctgcccg gcctggtgcg caacccagga cggccgggcc cggccccggg acaccagcgc
     3121 gcaaggaccc gccaactcta ccggccagag gggcaaatgt cggctttggc agcctcaagg
     3181 ccggtccttg ctccaccgct tgacgccgtc gcgacctggc gtcgtcgccc gatgggtcgc
     3241 ccgatgggtc ggccagctgg cgcgcgcccg cgtgtagtag cgtcggccag ctggcgcgcg
     3301 cccgcgcgta gt
//
LOCUS       DATAIX010000234         1636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_162076, whole genome shotgun sequence.
ACCESSION   DATAIX010000234 DATAIX010000000
VERSION     DATAIX010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162076"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VHM65_03510"
     CDS             complement(<1..473)
                     /locus_tag="VHM65_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2704799.1"
                     /translation="MTIDQPQAVQDDGARAPVVAGVDGTGNDELVLAWAAAEAQRLGV
                     ALLAVHALDLPSGVVAGGFSDRHEILATVRLERRGEAVRHALERVRTTHPDVETSMVD
                     RWGTPAQELVALGGRASAIVVGGGRKSGLAKLAVGSTSLAVAMHAPCPVVLVRAHT"
     gene            complement(521..>1636)
                     /locus_tag="VHM65_03515"
     CDS             complement(521..>1636)
                     /locus_tag="VHM65_03515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX2704800.1"
                     /translation="GFDGLWLSLAIGVVAAVVVAVWAGPICAVFGASPEAVGHATSYL
                     RISAAGIPAMLAVLAVTGVLRGLQDTRTPLVVSVLAFSTNVALNLAFVYGLGLGIVGS
                     AWGTVLAQTGMALALVTVVTRHARRLGARLAPHPVRVLRAARDGVPLMLRTIALRATL
                     LTATWVAARQGDIPLAAYQVSMTVWTFLVFALDALAIAGQALTGRSLGAGDVAGTRAM
                     TTLMIRWGIGAGVGFGLAVAALAGVLPAIFTSDPATRAAIGAALLAVAAAQPLSGWVF
                     VLDGVLIGAGDNRFLAWAQSVAFAAYAPVAWATYVLSDRLATPGARVQLLWWGVAVWM
                     LVRGVLLQRRASGTAWLRTGAVSTSVTRGELDPSRGS"
BASE COUNT          264 a          600 c          606 g          166 t
ORIGIN      
        1 gtatgggccc ggacgaggac gaccgggcag ggggcgtgca tggcgacggc gagagacgtc
       61 gaaccgaccg caagcttggc caacccggac ttgcggccgc ccccgaccac gatggccgat
      121 gcccgcccgc cgagggcgac cagctcctgg gccggggttc cccagcgatc gaccatcgag
      181 gtctccacgt cgggatgtgt cgtccgaacc cgctcgaggg cgtgccgcac ggcctcaccc
      241 cgtcgctcga gccgcaccgt cgcgaggatc tcgtgccggt cggagaagcc gccggcgacc
      301 acgccggacg gcaggtccag cgcgtggacc gccagcagag cgacgccgag tcgctgcgcc
      361 tcggcggccg cccacgcgag gacgagctcg tcattgccgg ttccgtcgac tccggccacc
      421 actggggcac gtgcgccgtc gtcctgaaca gcctggggct ggtcgatcgt catacctgca
      481 ggatgctcgc gcccagggcc ggccgacagg ggccttcgac tcacgaacct cgcgacgggt
      541 ccagctcccc gcgggtcacc gacgtcgaca ccgcgccggt gcgcagccag gcggtccccg
      601 aagcacgccg ttgcagcagg accccacgga cgagcatcca gacggcaacg ccccaccaca
      661 gcagctggac gcgcgcaccg ggcgtggcga gccgatcgct gaggacgtac gtcgcccacg
      721 cgaccggcgc gtacgccgcg aacgcgaccg actgcgccca ggccaggaac cggttgtccc
      781 cggccccgat gaggacgccg tcgaggacga agacccagcc cgacagcggt tgcgccgcgg
      841 cgacggcgag cagggccgcc ccgatcgccg cccgggtggc cgggtcgctg gtgaagatcg
      901 cgggaaggac cccggccagc gcggcgacgg ccagcccgaa gccgaccccg gcgccgatgc
      961 cccatcggat catgagtgtc gtcatggcgc gggtgccggc cacgtcaccc gcgccgaggc
     1021 tgcgcccggt cagggcctgc ccggcgatgg cgagcgcgtc gagcgcgaag acgaggaacg
     1081 tccacaccgt catcgacacc tggtaggccg cgagcgggat gtcgccctgg cgtgcggcca
     1141 cccacgtcgc ggtgagcagg gtggcccgca gggcgatcgt ccgcagcatg agcggtacgc
     1201 cgtcgcgtgc ggcgcgcagc acccgcaccg ggtgcggcgc caggcgggcg ccgaggcgtc
     1261 tggcgtggcg ggtgacgacc gtgacgaggg cgagcgccat accggtctgg gccaggaccg
     1321 tgccccaggc ggaccccacg atcccgaggc ccagaccgta gacgaacgcc aggttgaggg
     1381 cgacgttggt gctgaaggcg agaaccgaga caacgagcgg ggtcctggtg tcctgcagcc
     1441 cccgcagcac ccctgtgacg gcgaggacgg cgagcatcgc ggggatgccg gcagcggaga
     1501 tgcgcaggta tgacgtcgcg tgccccaccg cctcggggga ggcaccgaag acggcgcaga
     1561 tcggtccggc ccagacggcg acgacgacgg cggcgacgac gccgatggcc agcgacagcc
     1621 agagtccgtc gaaccc
//
LOCUS       DATAIX010000235         4554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_184442, whole genome shotgun sequence.
ACCESSION   DATAIX010000235 DATAIX010000000
VERSION     DATAIX010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184442"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VHM65_03520"
     CDS             complement(<1..193)
                     /locus_tag="VHM65_03520"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEX2704801.1"
                     /translation="MPRYLVERTFPGGLAIPMNSSGAKSCLGVVDGNATKGVTWVHSY
                     VTPDREKTFCVYDGPDPEAI"
     gene            complement(268..2871)
                     /locus_tag="VHM65_03525"
     CDS             complement(268..2871)
                     /locus_tag="VHM65_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2704802.1"
                     /translation="MDLVEREAPLAVLSALLVEARRGHGSLVLLGAEAGMGKSSVLRA
                     FADHAAGSGTRVLWGSCDPLTTPRPLGPLWDMTDADPALNQRLDVDGARHQLFSAVLH
                     ELSSPTVMAVEDLHWADDATLDLVRFLGRRVAATRSVVVLTYRDEHPPTPLRSVLGDL
                     ATSAGVRRLTLDPLTSQGVAEVIGDQPLDPEHIRRLTAGNPFYVTEVATAPGWTVPPT
                     VADAVLARIAPRSAPARELLGAVSESPGGLELELVEALVPGSEAALDECVGSGMLIES
                     GGRVAFRHEIARLAVRDDQLPARRRAVSLRLLELLEERASRDEARLAHHAQVAGDRER
                     VVRYCAAAGAAASARGAHRAAAQQYAAAVTHADGLPEERRVALLSLWATERNSLGDAA
                     EVARLWRQIAGDWRAVGDQVRAAAAHLECARSLWSTGDSAGARRLAEGAVAALEVCPP
                     GPELALGYARLASLSMLARDAEAALRWGHRAIELAENVGPSATRALAAALNATGSTLI
                     VSHESVDGVADLSRSHDLALGLGDDVGACLALSNLGTALGEVRHYPQASEYLTRAVEF
                     ARARDLDDAHGYAAAWLARVLFETGRWDEAVEQAQYSLDSGGTMPIIAITALTVQARV
                     RVRRGEPGHQSLLDEASRLADATGDLQRLWPVAAAHAEALWLAERRDDVVALVQNHLA
                     TAERLGIRWAVGELAFWRWRAGHPAPPSDAAAEPYRLHTLGDWSGAARAWEQLGCPYE
                     QAECLADGDEEAQRAALAAFDRLGARPAADRLRGRMRAAGLTGIPSRPRAATRAAPQQ
                     LTARQLEVLALVAEGRSNAAIAQRLFISEKTAGHHVSAVLHKLAARSRGEAAATALRL
                     GIVLPEAAGET"
     gene            3533..3763
                     /locus_tag="VHM65_03530"
     CDS             3533..3763
                     /locus_tag="VHM65_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX2704803.1"
                     /translation="MKLSVSLSEDEVAALDKYARAAGLASRSAAIQQAIRLLGDPELE
                     DSYAAAWDEWETSGDAAAWDSTAADGLLDAAR"
     gene            4261..>4554
                     /locus_tag="VHM65_03535"
     CDS             4261..>4554
                     /locus_tag="VHM65_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019750077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2704804.1"
                     /translation="MFKVAQEKDQPTAHIPAGVGGSLLDELVRDGARAMLAAALQAEV
                     SAYIDAHTEHVDQAGHRLVVRNGHHQPREVATAAGAVPVRAPRVNDKRIDEATG"
BASE COUNT          663 a         1630 c         1651 g          610 t
ORIGIN      
        1 tgatcgcctc ggggtcggga ccgtcataca cgcagaacgt cttctcgcgg tcgggtgtca
       61 cgtacgagtg cacccacgtc acgcccttgg tcgcgttgcc gtcaacgacg ccgagacagg
      121 acttggcgcc gctgctgttc atcggtatgg cgagaccgcc gggaaacgtc cgttccacga
      181 gatacctggg catgtgccct ccttttgcgg gtgatggtgc cagcaaaggt aggtgtgtcg
      241 agtgcacgcc ttcgtcggga gatctcccta tgtttcgccc gcggcctccg gaagcacgat
      301 gccgagccgc agggccgtgg cggcagcctc tccgcgcgac cttgcggcca gcttgtgcag
      361 cacggcggac acgtggtggc ccgcggtctt ctcggagatg aacagccgct gcgcgatcgc
      421 ggcgttgctg cgaccctcgg cgacgagcgc cagcacctcc agctggcgcg cggtgagctg
      481 ctgcggcgcc gcccgggtcg ccgcccgcgg tcgagaaggt atgccggtca gcccggcggc
      541 gcgcatccgt ccccgcagcc tgtcggcggc ggggcgtgcc ccgagccgat cgaaggccgc
      601 cagcgcggcg cgctgcgcct cctcgtctcc gtcggccagg cactcggcct gctcgtaggg
      661 acaccccagc tgttcccagg cacgcgccgc gccagaccag tcgccaagag tgtgcagccg
      721 gtacggctcg gcggcagcgt cggagggtgg agcggggtgc ccggcacgcc agcgccagaa
      781 ggccagctcg ccgacggccc acctgatccc gagccgctcc gcggtcgcga gatggttctg
      841 cacaagggcg accacgtcgt cacgccgttc tgccagccag agtgcctcgg cgtgcgcggc
      901 cgccaccggc cagaggcgtt gcaggtctcc ggtcgcgtcg gcgagccggc tggcctcgtc
      961 gaggagcgac tggtggccgg gctcgccgcg gcggacccgc acgcgtgcct ggacggtcaa
     1021 cgccgtgatg gcgatgatcg gcatggtccc accggagtcg agcgagtact gcgcctgctc
     1081 aaccgcctca tcccaccgcc cggtctcgaa gagcacgcgg gccagccacg cggcggcata
     1141 cccgtgcgcg tcgtcgaggt cgcgcgcgcg ggcgaactcg accgcccggg tgaggtactc
     1201 gctcgcctgg gggtaatgcc tcacctcgcc gagcgcggtg ccgaggttgc tcagcgccag
     1261 gcaagccccg acgtcgtcgc cgagtccgag ggcgaggtcg tggctgcgcg acaggtccgc
     1321 gacaccgtcc acggactcgt gggagacgat gagggtcgag ccggtggcgt tgagcgccgc
     1381 ggccagtgcc cgcgtggcgg acggcccgac gttctcggcc agctcgatcg cccggtgacc
     1441 ccagcgcagg gcggcctcgg cgtcgcgggc gagcatggac agggacgcca gccgcgcgta
     1501 gccgagggcg agctcgggtc ccggcgggca gacctcgagc gccgcgacgg caccttccgc
     1561 cagccgccgc gcacccgcgc tgtccccggt cgaccacagg ctccgggcgc attcgaggtg
     1621 ggccgccgct gcgcgcacct ggtcgcccac cgctcgccag tcgccggcga tctggcgcca
     1681 cagccgggcg acctccgccg cgtcgcccag gctgttgcgc tcggtcgccc agagggacag
     1741 cagcgcgacc cggcgctcct ccggcaggcc gtctgcgtgc gtgactgcgg cggcgtactg
     1801 ctgggccgct gcccgatggg cgccgcgggc ggacgccgcg gcaccggcgg ccgcgcagta
     1861 gcgcaccacg cgctcgcggt caccagccac ctgggcgtgg tgggccagcc gcgcctcgtc
     1921 gcgcgacgcg cgctcctcga gcagttccag cagccggagg ctgaccgccc ggcggcgtgc
     1981 ggggagctgg tcgtcgcgga ccgccagccg ggcgatctcg tggcggaacg cgaccctgcc
     2041 gcccgactcg atgagcatgc ccgaacccac gcattcgtcg agcgcggcct ccgagccggg
     2101 gacgagcgcc tcgaccagct cgagctcgag gccaccgggg gactccgaga ccgctcccaa
     2161 gagctcccgg gccggtgccg agcggggcgc gatgcgcgcc agcacggcgt cggcgacggt
     2221 ggggggcacg gtccagccgg gggcagtggc cacctcggtg acgtagaagg gattgccggc
     2281 ggtcagcctg cggatgtgct ccgggtcgag cggctggtcg ccgatcacct cggcgacgcc
     2341 ctgcgaggtg agcggatcga gggtcagccg gcgcacgccc gcgctggtcg ccaggtcgcc
     2401 gaggacggag cgcagcgggg tgggcgggtg ctcgtcgcgg taggtcagca cgaccaccga
     2461 cctggtggcg gcgacccgtc gcccgaggaa ccggacgagg tcgagcgtcg cgtcgtcggc
     2521 ccagtgcagg tcttcgacgg ccatgaccgt gggggaggag agttcgtgca ggactgcgga
     2581 gaacagctgg tggcgggctc cgtcgacgtc gagccgctgg ttcagggctg ggtcggcgtc
     2641 ggtcatgtcc cacagcggcc ccagtggccg gggggtggtc agcgggtcgc agctgcccca
     2701 gagcacgcgc gtccccgagc cggctgcatg gtcggcaaac gcccgcagca cgctggactt
     2761 gcccatcccc gcctccgcgc ccaggagcac cagcgatcca tgtccccgcc tggcctcgac
     2821 gagcagggcg gagagcacag cgagcggagc ctctcgctcc acaaggtcca cccgatcacg
     2881 gtagcccggc ccatcccggc tggacagcga gtcccccggt ttcccgggtc gagccgggcc
     2941 cgtcgggagg aggcaacgga cccggccggg tctgttgggc gccgcgaact ccaagtgggc
     3001 gccgacgacg agggtccacg cgcggcccgg cccaggctcg gtgcccgtct cgtcgatctc
     3061 gaccgagccc acccgcgcga gccagtcgtc atacgggctg cgggcgaagt cgcggccccc
     3121 acacggccaa ccgtcgtccg tcggcgaact cgacgtcggt ggtcggctcg aagccgaggc
     3181 agctccagat cggcgcccac gtctcccttc gcgtcgtcct caggatcgac cacccgaggc
     3241 cgggcacggt cccctcgccg agacgatgga tcgccatgag actgacgacg gcgccgagct
     3301 tcctcaggcg gcaccctcgc gaccgcgaac tggtgcagga ccgtccaagc ctgagggcgc
     3361 ctcccgcgac tggttcaacg tacgagggct ccgcgccgac agcgggatga gcgggcgagc
     3421 gcagccgccc tcagcgcggt atgaccgtgc gagcacagcc gccctgccca gcggcatgag
     3481 cgaatgagcg accggagtag ccaggagaga actactggag tagcataggg tcgtgaaact
     3541 cagcgtcagt ctctccgagg acgaggtcgc cgccctcgac aagtacgcac gcgcggcagg
     3601 actggcgtcg cgttctgcgg cgatccagca ggcgatccgg ctacttggcg acccagagct
     3661 cgaggactcc tacgccgccg cctgggacga gtgggaaacg tcgggtgacg ctgcggcctg
     3721 ggactcgacc gcggccgacg ggctccttga tgctgcgcgg tgagatccgt ctgaccgact
     3781 tggagccgac ccggcctagc gaggcgaaca agcgtcgccc agccgtcatt gtcagcaacg
     3841 accgtgccaa cgccaccgcc gcacgactcg gtcgcggcgt ggtcaccgtc gttccggtca
     3901 ccagcagcgt ctcccgggtg ttccccttcc aggtgctcct accagccgac gagacgggcc
     3961 ttcgggtcga ctccaaggca caagccgaac aggtccgctc agtgtcagtc gaacgcctgg
     4021 gcccggtgat cggccgagct cccacgcacc tgatgactca gctcgacgac gccttgcgcc
     4081 tccaccttca gctctagctg gatgccatgt ccgcggcatg tgcggagagc aatgatcaga
     4141 acctgtggat ggtcctcggc caggtgacgc gggtgggcgc accttcccga ggatgatctg
     4201 aagcccaagc aagtctgatc aagagccggc gttgacgcgc tggttcggga aggtacgccc
     4261 atgttcaagg tagcccaaga gaaggatcag cccactgctc acatcccggc cggggtcggc
     4321 gggtcgctgc tggatgagct ggttcgtgac ggcgcgaggg ccatgctcgc ggccgccttg
     4381 caggccgagg tcagcgccta catcgacgcc cacaccgagc acgttgacca ggcgggtcac
     4441 cggctggtgg tccgcaacgg ccaccaccaa ccgcgggagg tggccacggc cgcgggtgcg
     4501 gtgccggtgc gggcgccgcg ggtcaacgac aagcgcatcg acgaggcaac gggt
//
LOCUS       DATAIX010000236         1763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_243945, whole genome shotgun sequence.
ACCESSION   DATAIX010000236 DATAIX010000000
VERSION     DATAIX010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243945"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1581)
                     /locus_tag="VHM65_03540"
     CDS             complement(73..1581)
                     /locus_tag="VHM65_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX2704805.1"
                     /translation="MRPRTDAPVPLEQARRRHAVTHPVAPVATRPARGPLAEQGEIDW
                     GLVRAFRTLVAGRLAQALGRDWRERGEEEDSPNGAVRAAMDRTAQEQYGWTVITDLLK
                     EHTADAVATGAQAWTAQQEAVVAQAVFDAVFRLGRLQPLVDADRVENIFIIGCRQVLL
                     ELVDGSRVPGPPVADSDEELIDFLRFLANRSESNPRPFSESTPKLHMKLDGGARLAAT
                     AWVVSSPTVVIRRHRLKVDTLSGWVERDGMSPSCANLLAAVVRGAWSATVSGTMSAGK
                     TTLMRAMCAQIPRDEAIGTFESDYELFLKEAGNHEVVIEWEARPGVGEIGPDGRAAGS
                     FELWEALIDSYRFALSRQIIGEIRGPEVWTMIKAMESGTGSISSTHAANAEACVRKLV
                     TCAMEAGGHVTHDLVVSKLAECLDVIVHVRRKVVHLPDGTQALRRWVSEVVVVEPGEK
                     EKGYALTHIFGPSEDGPAQATGILTERCKDLAEHGFDVDLYQREADGRGWRP"
BASE COUNT          255 a          629 c          618 g          261 t
ORIGIN      
        1 gcagcgcgag gaccatcccg accaggccgg ccacggtgag ggcaccaaag acagcgggca
       61 gcagctgagg ggtcacgggc gccacccccg cccgtcggcc tcgcgctggt agaggtccac
      121 gtcgaagccg tgctcggcca ggtccttgca ccgctcggtg agaatgcccg tggcctgggc
      181 ggggccgtcc tcgctgggcc cgaagatatg ggtgagggcg tagcccttct ccttctcccc
      241 cggctcgacg acgacgacct cgctgaccca tcgacgcagc gcctgggtgc cgtccggtag
      301 atgcacgacc ttgcggcgga catgcacgat cacgtcgagg cactccgcga gcttgctgac
      361 gaccaggtcg tgggtgacgt ggccgccggc ctccatggcg caggtgacga gcttgcgcac
      421 gcacgcctcg gcgttggcgg catgggtgga ggagatcgag ccggttccgg actccatcgc
      481 cttgatcatc gtccacacct cgggcccgcg gatctcaccg atgatctgcc gcgagagggc
      541 gaaccggtat gagtcgatca gcgcctccca cagctcgaac gagccggccg cgcgcccatc
      601 cgggccgatc tcgcccaccc cgggccgggc ctcccactcg atgacgacct cgtggttgcc
      661 ggcctccttc aggaacagct catagtcgct ttcgaaggtg ccgatcgcct cgtcacgcgg
      721 gatctgcgcg cacatcgcac gcatgagggt cgtctttccg gcggacatgg tccccgagac
      781 ggtcgcgctc cacgccccgc ggacgacggc ggccaagagg ttcgcgcacg aggggctcat
      841 gccgtcgcgc tcgacccacc ccgagagggt gtcgaccttg agccggtggc gtcggatgac
      901 gaccgtcggc gagctgacca cccacgcagt ggccgcgagg cgggcgccgc cgtcgagctt
      961 catgtgcagt ttcggggtgg actcggagaa cgggcgcggg ttggactcgg accggttggc
     1021 caagaagcgc aggaagtcga tcagctcctc atcactgtcg gccaccggtg gcccggggac
     1081 gcggctgccg tcgacgagct cgagcaggac ctggcggcat ccgatgatga agatgttctc
     1141 cacccggtcc gcgtcgacca gcggttggag cctgccgagg cggaacaccg cgtcgaacac
     1201 ggcctgggcc acaacagcct cctgttgcgc cgtccacgcc tgcgcgccag tggccaccgc
     1261 gtcggcggtg tgctccttga gcaggtcggt gatgaccgtc cagccgtact gctcctgggc
     1321 tgtgcggtcc atcgctgccc ggacggcccc gttcggggag tcctcctcct cgcctcgctc
     1381 acgccagtcc cggcccaggg cctgcgcgag gcggccggcc accagggtgc ggaaggcccg
     1441 caccagcccc cagtcgatct cgccctgctc cgccagcggc ccgcgtgccg ggcgcgtcgc
     1501 gaccggcgcg accgggtggg tcacggcgtg ccgacgccgg gcctgctcca acggaacggg
     1561 cgcatccgtg cggggcctca tcgagaaacc gccacggaca cgtccagacc gcggcgcggg
     1621 cgcggagaac agcggcagcg atgctgggtc gctcggatcg gtggagggaa tcagaggctc
     1681 gctagtcaca tctgcctccc ggtggtgctc gcctgcagct gctcaccgtt ggtgcggatc
     1741 gtcccggtga tggcgctgcg ggc
//
LOCUS       DATAIX010000237         3745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_12671, whole genome shotgun sequence.
ACCESSION   DATAIX010000237 DATAIX010000000
VERSION     DATAIX010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12671"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VHM65_03545"
     CDS             <1..562
                     /locus_tag="VHM65_03545"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704806.1"
                     /translation="GGLGRDELLELAAGLGSDVPFSLTGGIAIGSGRGELVTPVLAKG
                     SYHWVFALSEVGLPTPKVYAECDRLRAVTGTRVGNPRPGSALMTALRSGDPVALGRAL
                     VNDLEPAALSLQPDLREVLEVGREFGALGALVSGSGPTVALLGADVESSIDISVALTA
                     SGVVAEVRRAIGPVHGAHVVATPART"
     gene            562..2406
                     /locus_tag="VHM65_03550"
     CDS             562..2406
                     /locus_tag="VHM65_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX2704807.1"
                     /translation="MANLVSIERARIVFGTVAVLDGLSTGVGAGERIGVVGRNGGGKT
                     TLLRVISGVQPLDEGRVARAGGTDGIAVAMLSQDDRLDPADTVRAAVVGARPEHEWAG
                     DARIRDVLTGLLGGVEVPAVGGFEALVGPLSGGERRRVALAALLVADPDVLLLDEPTN
                     HLDIEGVAWLADHLVQRRTRAGVATVAVTHDRWFLDAFATTTWEVQGGAVAAFDGGYS
                     AYVLARAERERMAAVTAERRDNLLRKELAWLRRGPPARTSKPKFRIDAATVLIEDEPP
                     PRDTVELTRFASTRLGKDVIDLLDATVRLGDRTILDRVTWRLAPGDRVGIVGVNGSGK
                     TTLLRAISGDQPLDAGRRKVGKTVALAQLTQEVRELERFADWRVIDAVEDVRSFVSLG
                     GKEVSASSLAKRLGFPGGRQQDRVGDLSGGERRRLQLLRLLMAEPNVLLLDEPTNDLD
                     IETLTSLEDVLDGWAGTLLVVSHDRYLLERVTDRQVALLGDGRLRDLPGGVEEYLRRR
                     AEIRIADVAALGALAATSGSTPSASSTAAATGLGSAADVRAARKDMARIERALARLAT
                     QEERLHHDMAAAASDHTAIRDLDATLRDVIQEREALEHAWLEAAGLSG"
     gene            complement(2413..2919)
                     /locus_tag="VHM65_03555"
     CDS             complement(2413..2919)
                     /locus_tag="VHM65_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2704808.1"
                     /translation="MPHGPRDDVDSIVAAWVRERPDLDVGPLHVLSRVSRLARHLDLA
                     RGSAFAEHGLEGWEFDVLSALRRAGAPYQLSPGALVAQTLVTSGTMTNRVDRLEQRGY
                     VARSPDPTDRRGVIVTLTPRGAEAVDAAMADLLDRERELLSRLPEADRGTLAMLLSRL
                     LTPFETSA"
     gene            3037..>3745
                     /locus_tag="VHM65_03560"
     CDS             3037..>3745
                     /locus_tag="VHM65_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2704809.1"
                     /translation="MWDPAQYALFAGHRGRPFADLLARVDATSPRLVVDLGCGDGPLT
                     RGLAQRWPQARVVGIDSSPQMLAAARAGDVPARVEWLLERVETWDPDGLGTAIDVLVT
                     NATLQWVPGHLDLIPRWLDVLAPGGWFAMQVPANYDAPSHRLMREVASRHPRATDLTP
                     GLRRAAAVAEPAAYLELLSRPGLATDVWETTYLHVLDPEGTRRSPVLDWVRATGLRPV
                     LDVLTDRDELAAFLEDYA"
BASE COUNT          507 a         1373 c         1355 g          510 t
ORIGIN      
        1 cggaggcctc ggtcgggacg agctgctcga gctcgcggca ggtctgggca gcgacgtgcc
       61 gttctcgctg accggaggga tcgcgatcgg ctccggcagg ggtgagctcg tgacgccggt
      121 gctcgccaaa ggcagctacc actgggtctt cgcgctgagc gaggtgggac tgcccacccc
      181 caaggtgtat gccgagtgcg accgcttgcg agcggtgacc gggacccggg tcggtaaccc
      241 ccggcccgga agcgcgctca tgacggcgct gcgttccggt gaccccgtgg ccctgggccg
      301 ggccctcgtc aacgacctcg agcccgcggc cttgtcactg cagcccgacc tccgggaggt
      361 tctcgaggtc ggccgtgagt tcggggccct cggagcgctt gtctcggggt ccggccccac
      421 ggtcgcgctg ctcggcgccg atgtcgagtc ctcgatcgac atcagcgtcg cgctgaccgc
      481 gtcgggagtc gtcgccgagg tccggcgcgc catcggcccg gtgcatggcg cgcacgtcgt
      541 tgccacccct gccaggacct gatggccaac ctcgtctcga tcgagcgagc ccggatcgtc
      601 ttcggcaccg tcgccgtgct cgacggcctc tccacgggtg tgggcgcggg tgagcggatc
      661 ggcgtagtcg ggcgcaacgg gggcggcaag accacgctcc tgcgggtcat ctcgggcgtg
      721 cagccgctcg acgaggggcg ggtcgcccgg gccggcggga ccgacgggat cgccgtggcg
      781 atgctgagcc aggacgaccg gctcgacccg gcggacacgg tccgcgcggc ggtcgtcggc
      841 gcccgccccg agcacgagtg ggccggtgac gcccgcatcc gcgacgtcct caccgggctc
      901 ctgggcggcg tcgaggtccc agcggtcggt gggttcgagg cgctggtcgg cccgctctcg
      961 ggcggcgagc gccgccgagt cgccctcgcc gcactgctcg tcgccgaccc cgacgtcctc
     1021 ctgctcgacg agccgacgaa ccacctcgac atcgaaggag tggcctggct cgccgaccat
     1081 ctcgtccagc gccggacgag ggccggcgtc gcgaccgtcg ccgtaaccca tgaccgctgg
     1141 ttcctcgacg cctttgcgac gacgacatgg gaggtgcagg gcggtgccgt tgccgccttc
     1201 gacggaggct actcggccta cgtcctggcc agggccgagc gcgagcggat ggcggcggtc
     1261 accgccgagc gccgcgacaa cctcctgcgc aaggagctcg cctggttgcg ccgcggtcct
     1321 ccggctcgca cgagcaaacc gaagttccgc atcgatgccg cgaccgtgct catcgaggac
     1381 gaaccgccac cacgggacac ggtcgagctg acccgcttcg cctcgacgcg gctcggcaag
     1441 gacgtcatcg acctgctcga cgcgacggtc cgcctcgggg accggaccat cctcgaccgg
     1501 gtcacctggc ggctggcacc gggagaccgg gtcggcatcg tcggggtcaa cgggtccggg
     1561 aagaccacgt tgctgcgggc gatcagcggc gaccagcccc tcgacgccgg gcgccgcaag
     1621 gtcggcaaga cggtcgcgct cgcccagctg acccaggagg tccgcgagct cgagcgcttc
     1681 gccgactggc gggtcatcga cgcggtcgag gacgtgcgga gcttcgtcag cctcgggggc
     1741 aaggaggtca gcgcgagctc gctcgccaag cgtctcggct ttccgggggg gcgccagcag
     1801 gaccgcgtcg gtgacctctc cggcggcgag cggcggcgac tgcagctgct ccgcctgctc
     1861 atggccgaac ccaacgtcct cctcctcgac gagccgacga acgacctcga catcgagacg
     1921 ctgacctcgc tcgaggatgt cctcgacggg tgggccggca cgctgctcgt cgtctcgcac
     1981 gaccgctacc tgctcgaacg cgtcaccgac cgccaggtcg ccctgctcgg tgacggccgg
     2041 ctgcgggacc tgcccggcgg cgtcgaggag tacctgcgcc ggcgcgccga gatccgcatc
     2101 gccgatgtcg ccgccctcgg ggctctcgcc gccaccagcg gctcgacccc gtccgcgtcg
     2161 tcgacggccg cggcgaccgg gctcggctcc gccgccgacg tccgcgcggc gcgcaaggac
     2221 atggcgcgga tcgagcgagc cctggccagg ctggccaccc aggaggagcg cctccaccac
     2281 gacatggccg ccgccgcgag tgaccacacc gcgatccgcg acctcgacgc caccctgcgc
     2341 gacgtcatac aggagcgcga agcactcgag cacgcctggc tcgaagccgc cggcctgtcc
     2401 ggctgacggg actcaggccg aggtctcgaa gggggtgagc aggcggctca acagcatcgc
     2461 gagggtgccg cggtcggcct cgggcaggcg ggagagcagc tcgcgctcac ggtcgagcag
     2521 gtcggccatg gccgcgtcga cggcctcggc cccgcgcggc gtcagcgtca cgatgacgcc
     2581 gcggcggtcg gtcgggtcgg ggctgcgcgc gacatacccc cgttgctcga gccggtcgac
     2641 gcggttggtc atcgtgccgg acgtgaccag ggtctgggcc acgagagccc caggggagag
     2701 ctggtatggc gcgccggcgc gtcgcagtgc cgacaggacg tcgaactccc agccctcgag
     2761 gccgtgctcg gcgaaggccg agccgcgggc gaggtcgagg tggcgcgcga gccgggacac
     2821 ccgggacagg acatgcaggg ggccgacgtc gaggtcgggg cgctcccgga cccaggccgc
     2881 cacgatgctg tcgacgtcgt ctcgcggtcc gtggggcacg cgtcaagaat accctcttca
     2941 agtatcttga catcgagata aaatggaccg aaccttggac cgaaccttgg accgaacctg
     3001 ggagtggctc cctgcgtcgc ggaaaggact gacctcatgt gggacccggc ccagtacgcc
     3061 ctcttcgccg gtcatcgcgg acggcccttc gccgacctgc ttgcccgggt cgatgccacg
     3121 agcccccggc tcgtcgtcga cctcggctgt ggcgacggcc cgctcacccg agggctggcc
     3181 cagcgctggc cgcaggcccg ggtcgtcggg atcgactcgt cgccgcagat gctcgccgcg
     3241 gcccgggcgg gcgatgtgcc tgcccgggtc gagtggctcc tggagcgggt cgagacgtgg
     3301 gacccggacg gactcggcac ggcgatcgac gtcctcgtca ccaacgcaac cctgcagtgg
     3361 gtccccggcc acctcgacct catcccgcgc tggctcgacg tcctcgcacc cggtggctgg
     3421 ttcgcgatgc aggtcccggc caactacgac gcgccctcgc accggctcat gcgcgaggtc
     3481 gcgagccggc atccgcgggc caccgacctg acccctgggc tgcggcgggc cgcggcggtg
     3541 gccgagcccg cggcatacct ggagctgctg tccagaccgg gcctggccac cgacgtgtgg
     3601 gagacgacct acctgcatgt cctcgaccca gagggcaccc ggcgctcgcc agtcctcgac
     3661 tgggtccgtg cgacggggtt gcgacccgtc ctcgacgtgc tgaccgaccg ggacgagctg
     3721 gctgccttcc tcgaggacta cgcca
//
LOCUS       DATAIX010000238         1720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_102254, whole genome shotgun sequence.
ACCESSION   DATAIX010000238 DATAIX010000000
VERSION     DATAIX010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102254"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1720)
                     /locus_tag="VHM65_03565"
     CDS             complement(<1..>1720)
                     /locus_tag="VHM65_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX2704810.1"
                     /translation="CYTSPGSFTTKLADCANGETHGTAVAEAVSEMAPGIDLYVADPI
                     SPLDDQRAVAWMTGNGVRIVNASFFSGQIFEGPGDGTTPYDGSLYSLVDQAVAGGALW
                     VNAAGNAGDSGWTGAWTDADGNDWLDFAPGVETDSMTMASGSDVTVAIRWADPWGASA
                     NNYDLALYSGSTRVAWSTDVQDGNGDPFEVLEFQAETPGRYDIKIRRVSGAPTSRMQL
                     LVGTSENVRLSHRVTEGTLPTPVDSVNPGMVAVGAVDVDRPTEIEPYSSRGPTLDGRI
                     KPDLVAADCAPTTTDSPFCGTSEAAPFVAGAAAQVLQVHPGMTPGQLAEWLRSHAVPL
                     GSPTPNSTFGAGRLDLGALPFEPPAGLAFAWAPTGAVAGAPLTGQPALTIVDAAGLVV
                     EAGPGSTSSVSISLAANPTGATLSCAGGPTRTAAAGAATFAGCSIDQPGTGFVIRAEA
                     VGLPPVLSAPFDILSAGSELPLRLTAGPSTITVGAGTVLTARFAPAPPTVTRPLEVER
                     SIDGLRWSSIGSRTTDETGALRVSEKPSVTGWYRVRFAGGAGVPAATSYPVRVVVRLA
                     IVRGAWT"
BASE COUNT          235 a          612 c          635 g          238 t
ORIGIN      
        1 ggtccatgcg ccgcggacga tcgcgagccg cacgacgacc cggacggggt agctcgtggc
       61 agcgggcaca cccgcgccgc cggcaaagcg cacccggtac cagccggtca cgctcggctt
      121 ctcgctgacc cgcagggcgc ccgtttcatc cgtcgtccgg ctgccgatgg acgaccagcg
      181 gaggccgtcg atcgagcgct cgacctcgag cggccgggtc acggtgggcg gtgccggcgc
      241 gaaacgcgct gtcaggaccg tgccggcgcc gaccgtgatc gtggacgggc cggccgtgag
      301 cctgagcggg agctcggacc cggcgctgag gatgtcaaac ggcgccgaca acacgggtgg
      361 cagcccgacc gcctccgccc ggatcacgaa gcccgtcccc ggctggtcga tcgagcaccc
      421 ggcgaacgtc gcggcgcccg cggccgcggt tcgggtcggc ccgccggcgc aggagagggt
      481 cgcgcccgtg gggttcgccg cgagcgagat cgagacgctc gaggtgctgc ccgggcccgc
      541 ctcgacgacg agcccggcgg cgtcgacgat cgtcagggct ggctggccgg tcagcggcgc
      601 gcctgcgacg gcgccagtcg gcgcccacgc gaacgcgagc cccgccgggg gctcgaacgg
      661 gagcgcgccg aggtccaggc ggccggcccc gaacgtggag ttgggggtcg ggctgccgag
      721 cgggacggcg tgggagcgga gccactcggc aagctggccc ggggtcatgc ccgggtgaac
      781 ctgcagcacc tgcgccgcgg ctcccgccac gaacggcgcg gcctcgctcg tcccgcagaa
      841 ggggctgtcg gtcgtcgtgg gggcgcagtc ggccgcgacg agatcaggct tgatccgccc
      901 gtcgagcgtc gggccgcgcg agctgtacgg ttcgatctcg gtcggtctat cgacgtccac
      961 ggcacccacg gcgaccatcc ccgggttcac gctgtcgacc ggggtgggca gggttccctc
     1021 ggtgacccga tggctgagcc tcacgttctc gctcgtgccc accaggagct gcatgcgcga
     1081 cgtcggtgcg cccgagacgc gccggatctt gatgtcgtac cgacccgggg tctcggcctg
     1141 gaactcgagg acctcgaacg ggtcgccgtt gccgtcctgc acgtccgtcg accaggcgac
     1201 ccgcgtcgac ccggagtaga gcgccaggtc gtagttgttc gccgatgcgc cccacggatc
     1261 ggcccaccgg atggccacgg tcacgtccga gccgcttgcc atggtcatgc tgtccgtttc
     1321 gacaccaggg gcgaagtcga gccagtcgtt gccgtcggcg tcggtccagg cgcccgtcca
     1381 ccctgagtcg cccgcgtttc ccgccgcgtt cacccagagg gccccgccgg ccactgcctg
     1441 gtcaacgagc gagtacaggg agccatcgta cggcgtggtg ccatcgccgg gaccctcgaa
     1501 gatctgtccc gagaagaacg acgcgttgac gatgcggacc ccgttgcccg tcatccaggc
     1561 gacggctcgc tggtcgtcga gtggcgagat cgggtcggcc acgtacaggt cgatgccggg
     1621 cgccatctcg gataccgcct cggcgacagc cgttccatgg gtctcgccgt tggcgcagtc
     1681 agcgagcttc gtcgtgaagg atcccggtga ggtgtagcag
//
LOCUS       DATAIX010000239         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_273867, whole genome shotgun sequence.
ACCESSION   DATAIX010000239 DATAIX010000000
VERSION     DATAIX010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273867"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VHM65_03570"
     CDS             complement(<1..206)
                     /locus_tag="VHM65_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704811.1"
                     /translation="MPGTNLTREEALTRAALVHVDRYDVELDLTTGAETFRSVTTVHF
                     TCARPGAQTFIDLVSASVEAVTLNG"
     gene            281..967
                     /locus_tag="VHM65_03575"
     CDS             281..967
                     /locus_tag="VHM65_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbA"
                     /protein_id="HEX2704812.1"
                     /translation="MSAARAWIEQRAAPDRRSVSTAPWLNPGMTTPAEMWFDPICPWA
                     WMTSRWLMEVERVRGIAVTWSVMSLAVLNDGREVPEPYAERMKSAWGPVRVITAAARD
                     HGASVMKPLYDAMGTRIHLGQDKDLGRVIEESLAQCGLPADLARYAGSDEVDDLLRAS
                     HQRGIGLVGQDVGTPVIAVDGVAFFGPVVSPAPQGEAAGRLWDGCVLVAGTPGFYELK
                     RTRTIGPIFE"
     gene            1007..1489
                     /locus_tag="VHM65_03580"
     CDS             1007..1489
                     /locus_tag="VHM65_03580"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HEX2704813.1"
                     /translation="MRIHIGGDHAAYELHTALAGHLRGAGHDVADHGPYSFDPLDDYP
                     VFVLRAAQAVAAEPGSLGVVLGGSGNGEQIAANKVRGVRAALAWTPELARLGREHNDA
                     RVVSIGALFTSVAEAVAIVDAFLATPFTDDGRHLRRIAMIESYEGSGTLPALPTPDVG
                     "
     gene            1500..>1950
                     /locus_tag="VHM65_03585"
     CDS             1500..>1950
                     /locus_tag="VHM65_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704814.1"
                     /translation="MGEPWRGDVYVVRRSRRTVFARLFRDGRLVRWIGAMRVGAFLAA
                     ALLVAWGVVTWPMQVPWGGFAPLVVLAGILLSPGELMLTYAGFLTIVGVVGALVRPAQ
                     HDLLGGVVVILGVMGPMTWLSASRSRLGLRGSSGETMLIDLRDRLRAT"
BASE COUNT          249 a          659 c          727 g          315 t
ORIGIN      
        1 ccgttgaggg tgaccgcttc gacggaggcg ctgacgaggt cgatgaacgt ctgcgcgccc
       61 gggcgggcgc aggtgaaatg gacggtcgtc acgctgcgga acgtctcggc gccggtcgtg
      121 aggtcgagct cgacgtcgta gcggtcgacg tggaccaggg cggcgcgggt cagcgcctcc
      181 tcacgggtga ggttggtgcc gggcacgggg ttgactctcc tgaacggggg cagagtcccg
      241 ggctcacccg ggcactggat ctggcccatc ctcgcacggg gtgagcgccg cgcgagcctg
      301 gatcgagcag cgcgccgccc cggatcggcg ctcggtcagc acggctccgt ggttgaatcc
      361 cggtatgacg acaccggccg agatgtggtt cgacccgatc tgcccgtggg catggatgac
      421 ctcgcgctgg ctcatggagg tcgaacgggt ccgggggatc gcggtgacct ggtcggtcat
      481 gagcctggcc gtgctcaacg acggcagaga ggtgcccgag ccgtacgccg agcggatgaa
      541 gtccgcctgg ggacccgtgc gggtcatcac cgccgcggcg cgtgaccacg gggcgagcgt
      601 catgaagccc ctgtatgacg cgatgggcac gcgcatccat ctcggccagg acaaggacct
      661 ggggcgggtc atcgaggagt ccctcgccca gtgcgggctg cccgcagacc tggctcggta
      721 tgccggctcg gacgaggtcg acgacctgct ccgggcatcg caccaacgcg ggatcggcct
      781 ggtcggccag gatgtcggga ccccggtcat cgcggtcgac ggcgtggcgt tcttcgggcc
      841 cgtggtgagc ccggcgcccc agggcgaggc ggccggccgg ctgtgggacg gctgtgtcct
      901 cgtcgccggg acgcccgggt tctacgagct caagcgcacg cggaccatcg gcccgatatt
      961 cgagtgaccc tcaacttccc tggcaacaca cgaaggatca cgacgcatgc gcatacacat
     1021 cggcggtgac cacgcggcat acgagctgca cacggccctg gccgggcacc ttcgcggagc
     1081 gggccacgac gtggccgacc acggcccgta ctccttcgac ccgctcgacg actacccggt
     1141 cttcgtcctg cgggcggcgc aggccgtggc cgccgagccg ggctcgctcg gcgtggtcct
     1201 gggcggctcg ggcaacggtg agcagatcgc ggccaacaag gtcagggggg tgcgggccgc
     1261 cctcgcctgg actcctgagc tggcccggct ggggcgggag cacaacgacg cccgggtcgt
     1321 ctcgatcggg gcgctgttca cctccgtcgc ggaggccgtc gcgatcgtcg atgcgttcct
     1381 cgcgacgccg tttaccgatg acggtcgtca tctgcggcgg atcgccatga tcgagtccta
     1441 cgaggggtcc gggacgctgc cggccctgcc gacgcccgac gtcggctgag ggtcgcccga
     1501 tgggtgaacc ttggcgcggt gacgtctacg tcgtgcgccg cagccgcaga accgtgttcg
     1561 cccggttatt ccgcgacggt cgtctggtgc gctggatcgg cgcgatgcgt gtcggggcct
     1621 tcctcgccgc ggcgctgctc gtcgcgtggg gggtcgtcac ctggcccatg caggtcccgt
     1681 ggggcggctt cgcccctctc gtggtcctgg ccgggatcct gctctcgccg ggggagctca
     1741 tgctcaccta cgccgggttc ctgacgatcg tgggcgtcgt cggggccctc gtgcgtccgg
     1801 cccagcacga cctgctcggt ggtgtcgtcg tcatcctcgg cgtgatgggc ccgatgacct
     1861 ggctgtcggc gtcccggtcc cgactgggtc tgcgcggcag cagcggggag accatgctca
     1921 ttgacctccg tgaccgcctc cgcgcgactg
//
LOCUS       DATAIX010000240         1702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_102290, whole genome shotgun sequence.
ACCESSION   DATAIX010000240 DATAIX010000000
VERSION     DATAIX010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102290"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /gene="prpE"
                     /locus_tag="VHM65_03590"
                     /gene_synonym="yahU"
     CDS             <1..398
                     /gene="prpE"
                     /locus_tag="VHM65_03590"
                     /gene_synonym="yahU"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="catalyzes the formation of propionyl-CoA using
                     propionate as a substrate; PrpE from Ralstonia
                     solanacearum can produce acetyl-, propionyl-, butyryl- and
                     acrylyl-coenzyme A, and Salmonella enterica produces
                     propionyl- and butyryl-coenzyme A; not expressed in
                     Escherichia coli when grown on propionate/minimal media;
                     ATP-dependent; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HEX2704815.1"
                     /translation="VLNIAGHRLSTGSIEEALAGHPAVAECAVIGVHDDLKGQLPRAL
                     VVLKSGIDAKTDGVRICTELVARVRSEVGAVAALRQVDIVAALPKTRSGKILRKTMRE
                     IADGHTPSVPGTIEDASVLVTLTPVLLSH"
     gene            complement(548..>1702)
                     /locus_tag="VHM65_03595"
     CDS             complement(548..>1702)
                     /locus_tag="VHM65_03595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2704816.1"
                     /translation="NGGWVSALFPVGPDILARRYGEAAARAQLAAMRDTVDEVGRVVQ
                     AEGIDAGFLKSGTLVTARGPAQVTRAREDVEASRYWGSGTTWLGPGEARDRIRMTALL
                     GATFNPNCARVQPFRLAVGLGAACVRLGVDIREGSPVADAGRGLVRTVRGPVVRARHV
                     IRATEAWTARLPRLRRHLAPVYSLVVATEPLPASAWDEIGLAGREVFGSHQHVIVYGQ
                     RCTDDRLVFGGRGAPYHLGSGIGPDFDEDETVFASLRRTVGTLFPVLREATFTHCWGG
                     PLGVARDWHPAVHYDPASGVGWAGGYVGDGVATANLAGRTLADLVTGRSTPLTALPWV
                     GHDSPLWEPEPARWLGVNAGLQLARLADMEERITGHPALLGRAVSALTGH"
BASE COUNT          240 a          641 c          607 g          214 t
ORIGIN      
        1 acgtcctcaa catcgccggg caccgcctct cgacgggctc gatcgaggag gccctggccg
       61 ggcatccggc ggtcgccgaa tgcgccgtca tcggcgtcca cgacgacctc aagggccagc
      121 tgccccgcgc gctcgtcgtc ctcaagtccg gcatcgacgc caagaccgac ggggtgcgca
      181 tctgcaccga gctcgtcgcc cgggtccgct ccgaggtcgg cgccgtcgcg gccctgcgcc
      241 aggtcgacat cgtcgcggca ctgccgaaga cgcgctcggg caagatcctg cgcaagacga
      301 tgcgcgagat cgccgacggt cataccccgt cggttcccgg gacgatcgag gacgccagcg
      361 tcctggtcac actcacgccg gtcctgctgt cccactgacg tcggtcctgc tgtcccactg
      421 cgcccggtcc gcgtgccgca aagttgcacc tcgcgacgca tattcgcggt gcgaggtgtc
      481 aggatgcagc gcgaggttgt cacgccgcgg ccggcccact ggccccactg gccactggct
      541 cagggggtca gtggccggtc agggcggaga cggcgcgacc gagcagggct gggtggcccg
      601 tgatgcgctc ctccatgtcg gcgagccgag cgagctgcag accggcgttg acgccgagcc
      661 agcgggcggg ttccggctcc cacaaaggac tgtcgtgccc gacccagggc agtgccgtga
      721 gtggcgtgga tcgacccgtg accaggtcgg ccagcgtgcg tccggccagg ttggccgtcg
      781 cgaccccgtc accgacatac cccccggccc agccgacgcc gctcgccggg tcatagtgga
      841 cggcggggtg ccagtcgcgc gcgacgccga gcgggccgcc ccagcagtgg gtgaacgtgg
      901 cctcgcgcag gaccgggaac agcgtgccga ccgtgcgccg tagcgatgcg aagaccgtct
      961 cgtcctcatc gaagtccggg ccgataccag agccgagatg gtatggcgcg ccgcgccctc
     1021 cgaagacgag ccggtcatcg gtgcagcgct ggccgtagac gatgacgtgc tggtgcgacc
     1081 cgaagacctc gcgcccggcg agcccgatct cgtcccaggc cgacgcgggc agcggctcgg
     1141 tcgcgacgac cagggagtag accggggcga ggtggcgccg cagccgcggg aggcgtgccg
     1201 tccaggcctc ggtcgcccgg atgacgtgcc gggcccggac cactgggccg cgaacggtcc
     1261 gcaccagccc ccgccccgcg tcggcgacgg gcgagccctc gcggatgtcg acgccgaggc
     1321 gcacgcaggc ggctcccaga cccaccgcga gccggaaggg ctgaacccgc gcacagttcg
     1381 ggttgaacgt cgcgcccagc agggcggtca tccggatgcg gtcccgcgct tcgcccgggc
     1441 cgagccaggt ggtgcccgag ccccagtagc gcgacgcctc gacgtcctcc cgggcccggg
     1501 tgacctgcgc tggaccgcgt gcggtcacca gggtcccgct cttgaggaac ccggcgtcga
     1561 tcccctccgc ctgcaccacg cggccgacct cgtcgaccgt gtcgcgcatc gccgcgagct
     1621 gcgcgcgggc cgcggcctcg ccataccgcc tggccaggat gtcgggcccg accgggaaga
     1681 gcgccgacac ccacccgccg tt
//
LOCUS       DATAIX010000241         1934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_214069, whole genome shotgun sequence.
ACCESSION   DATAIX010000241 DATAIX010000000
VERSION     DATAIX010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214069"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VHM65_03600"
     CDS             complement(<1..542)
                     /locus_tag="VHM65_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX2704817.1"
                     /translation="MTATSPGPGSPVQGELAGMPKRLYGASPSRLLTWLDCPRRYRMA
                     YLDRPRPKARPPRAHTSLGVAVHSALRDFWDVPIGRRLPARGDELVRRSWVSAGFRDA
                     EQSERWRAASATHVTSYLQGVDPARQPVGVERTVAFKTPALAVVGRIDRLDERPTADG
                     PGSELVVVDYKTSRSAPSAE"
     gene            complement(552..1178)
                     /locus_tag="VHM65_03605"
     CDS             complement(552..1178)
                     /locus_tag="VHM65_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX2704818.1"
                     /translation="MRQAVDLDAVRTFGSVFITLLVIMDPPGALPIFLALTAQMPPSK
                     RVAAARRASLVAFGVIAMFAIFGQQILNYLHISIAALQGSGGLLLLLVALQLLTGQES
                     EQYAESGANVALVPLGTPLLAGPGAIVAIMLAVQRSDGAGELAATALGFTAAMVVVWV
                     FLRFASRIRRVLRESGTVLATRIAGLLLSAIAVQMVADSVRAFVTAAA"
     gene            complement(1175..>1934)
                     /locus_tag="VHM65_03610"
     CDS             complement(1175..>1934)
                     /locus_tag="VHM65_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX2704819.1"
                     /translation="LTTVAITDHDTTEGWADASAAARHHGIALVPGVEISCTRDGASV
                     HLLGYLVDPADAPLTDALARVRISRRTRMQAIVELMAADGIPITYDEVLAQVQPGATL
                     GRPHLADALIASGIVRTRDEAFAGWLHNSSRYYVRHYAPDAADAIRLVRGAGGVAVLA
                     HPFPTARGYAVTPALVEELAAAGLTGIEVGHRAHPPDVQASTRDLSRRLGLVETGSSD
                     YHGIGKVNRLGEHTTDADALAEIEARASGVAVIR"
BASE COUNT          280 a          655 c          746 g          253 t
ORIGIN      
        1 tcctcggcgg acggcgcact ccgggacgtc ttgtagtcga cgacgacgag ctcgctgccc
       61 gggccgtcgg ccgtgggtcg ctcgtcgagc cggtcgatgc gacccaccac ggccagggcg
      121 ggggtcttga acgccaccgt gcgctcgacc cccaccggct ggcgggctgg gtcgacgccc
      181 tgcaggtatg acgtcacgtg cgtcgcgctc gccgcgcgcc accgctcgct ctgctcggcg
      241 tcccgaaagc ccgcggagac ccacgaccgg cggaccagct cgtcaccacg cgcggggagt
      301 cgccggccga tcggcacgtc ccagaagtcc cgcagcgcgc tgtggaccgc gacgccgagg
      361 ctggtgtgcg ctctgggtgg ccgggccttg gggcgcggcc ggtcgagata ggccatccgg
      421 tagcgccggg ggcagtcgag ccacgtgagc aggcggctcg ggctcgcgcc atacaaccgc
      481 ttgggcattc ccgcgagctc gccctgcacg ggcgagccgg gcccgggcga ggtagcggtc
      541 accgcctcac cctatgcggc cgcggtgacg aacgcgcgga cactgtcggc gaccatctgg
      601 accgcgatcg ccgacaggag cagcccggcg atccgggtcg cgagcacggt gccgctctcg
      661 cgcagcaccc ggcggatccg gctggcgaag cgcaggaaga cccacacgac gaccatggcc
      721 gcggtgaacc cgagggcggt tgcggccagc tcacccgcgc cgtccgagcg ctgcacggcg
      781 agcatgatcg ccacgatggc tccgggtccg gcgagcaggg gggtgcccag tggcacgagc
      841 gcgacgttgg cgccggactc ggcatactgc tcggactcct ggccagtgag cagctgcagt
      901 gcgaccagga gcagcagcag accacccgat ccctgcaggg cggcgatcga gatgtgcagg
      961 tagttgagga tctgctggcc gaagatcgcg aacatcgcga taaccccgaa cgccaccagg
     1021 gaggcccgcc gggccgcagc gacgcgcttg ctgggcggca tctgcgccgt gagggccagg
     1081 aagatcggca gcgccccggg cgggtccatg atgacgagca gggtgatgaa gaccgagccg
     1141 aaggtccgga ccgcatcgag gtcgaccgcc tgcctcatcg gatgaccgcc acgccggatg
     1201 cgcgggcttc gatctcggcc agcgcatcgg cgtcggtggt gtgctcaccg agccggttga
     1261 ccttgccaat gccgtggtag tcgctcgacc ccgtctcgac cagacccagc cgccgggaca
     1321 ggtcccgggt cgatgcctgg acgtccgggg ggtgggcgcg gtggcccacc tcgatcccgg
     1381 tgagccccgc ggcggcgagc tcctcgacga gggcgggtgt gacggcatac ccgcgcgccg
     1441 tggggaaggg gtgagccagg accgcgaccc caccggctcc acggacgagg cgtatggcgt
     1501 cagccgcgtc gggtgcgtaa tggcggacgt agtagcggct cgagttgtgc agccagccgg
     1561 cgaaggcctc gtcgcgggtg cggacgatcc ctgaggcgat gagggcgtcg gcgaggtggg
     1621 gccgcccgag ggtggcgccg ggctggacct gggccaggac ctcgtcgtag gtgatcggga
     1681 tgccgtcagc ggccatgagc tcgacgatgg cctgcatccg ggtccgccgt gagatccgga
     1741 ctcgcgcgag agcgtcggtc aatggtgcgt cggccgggtc gacgaggtag ccgagcaggt
     1801 ggaccgacgc gccgtcgcgg gtgcaggaga tctcgacgcc gggcacgagg gcgatgccgt
     1861 ggtgccgtgc ggctgcgctg gcgtcagccc agccctcggt cgtgtcgtgg tcggtgatcg
     1921 cgacggtcgt cagg
//
LOCUS       DATAIX010000242         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_102551, whole genome shotgun sequence.
ACCESSION   DATAIX010000242 DATAIX010000000
VERSION     DATAIX010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102551"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..366)
                     /locus_tag="VHM65_03615"
     CDS             complement(130..366)
                     /locus_tag="VHM65_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704820.1"
                     /translation="MLILLPPSEGKAGPQRGRPVDLAALPFPELAATQQRVLSALAQA
                     SARPDAADVLRVSPRLGEEIALNTRLRTAPMSMT"
     gene            434..1873
                     /locus_tag="VHM65_03620"
     CDS             434..1873
                     /locus_tag="VHM65_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX2704821.1"
                     /translation="MVSRQVRLRRDPSAEGRSPLQRRFDAIRTDLEVPGPFRADVLEE
                     TRAAIAAVRLPDRDETALPFVTIDPPGSMDLDQAMHLTRSGSGFRVRYAIADVAAFVA
                     PGGALDREARHRGQTIYAPDDRTPLHPAELSEGAASLLPGQVRPAYVWDFTLDSAGVE
                     TLTDVYRAAVRSVERLDYEGVQAALDSGSTDERFVLLREVGLARIEQQRLRGGSSLSM
                     PEQEVIEGEDGSFSLHYRPPVPAEDWNAQFSLMTGMAAAHHMMEAKVGILRTMAVPRP
                     EAVALFRRQAAALGVPWEGGTTYGQFMRSLDRGNHKHLALTHQATRLFHGAGYTAVDG
                     EVPLANSHAAVAAPYAHVTAPLRRLADRFGLVVSECVSRGAEVPDWVRAALPDLPAVM
                     AISDKRAHAVGRACVDAVEAAVLSSRVGETFAAWVVDEAPNHEVAVQLADPAILTLAS
                     GKAGLGDEVRVELLEADPLTGVVRMNLLG"
     gene            2108..>2428
                     /locus_tag="VHM65_03625"
     CDS             2108..>2428
                     /locus_tag="VHM65_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2704822.1"
                     /translation="MTGTKEPDAEADRGPLHRRPWLASYAPGVPTDVDIPTGSLHEML
                     ADSARRYAGRAALDFFGAQTSYADLADQVSLAAEALHALGVRHGDRVALVLPNCPQHI
                     VAFYA"
BASE COUNT          340 a          887 c          869 g          332 t
ORIGIN      
        1 gagcaccgcc aggcgggtga acgttccgcg ccagaacccc gatcgagatc cggtccggca
       61 gccgttccta ccctgctctt gcctcacgag tcacaccagc cacacgcgac ccttagacca
      121 taaccacccc taagtcatgg acattggggc cgtccgcagg cgcgtgttga gggcgatctc
      181 ctcgccgagg cggggtgaga ctctcagcac atcggcggcg tcgggacgcg cactggcctg
      241 cgcgagagcg ctgaggacgc gctgttgggt ggccgccagc tcggggaacg gcagagcggc
      301 gaggtcgacg ggacgccccc gctggggccc ggccttgccc tcggagggcg ggagcaggat
      361 cagcaccccg ccacgatagg gctccgcgtc acaggaccag cgccggggct cggctggtgc
      421 gtactgtggg gccatggtct cccgacaggt gcgcctccga cgtgacccca gcgccgaagg
      481 gcgttccccc ttgcagcgcc ggttcgacgc catcagaaca gatctcgagg tgccagggcc
      541 gttccgagcg gacgtcctgg aggagacccg ggcggccatc gcggcggtcc ggctcccgga
      601 ccgggacgag acggcgctgc ccttcgtcac catcgacccg ccggggtcca tggacctgga
      661 ccaggcgatg cacctcaccc gctcgggatc gggcttccgg gtgcgctatg ccatcgccga
      721 cgtcgcggcc ttcgtcgcac cgggcggggc gctcgaccgc gaggcccgcc accgcggcca
      781 gacgatctac gcccccgacg accggacccc gctgcacccc gccgagctga gcgagggcgc
      841 cgcgagcctg ctgcccgggc aggtccgccc tgcctacgtc tgggacttca cgctggactc
      901 ggccggggtg gagaccttga ccgatgtcta ccgggctgcc gtccgctcgg tcgagcggct
      961 cgactatgag ggcgtccagg ctgcgctcga ctcgggcagc acggacgagc gcttcgtcct
     1021 gctccgcgag gtcgggctgg ccaggatcga gcagcagcgc ctccgcggag gctcgagcct
     1081 gtcgatgccg gagcaggagg tcatcgaggg cgaggacggc tcgttctccc tgcactaccg
     1141 cccgccggtg cctgccgagg actggaacgc ccagttctcc ctcatgaccg gtatggcggc
     1201 cgcccaccac atgatggagg cgaaggtcgg catcctgcgg acgatggcgg tgccccggcc
     1261 cgaggcggtt gccctgttcc ggcgccaggc cgcagccctg ggcgtgccct gggagggggg
     1321 gacgacatac ggccagttca tgcgttcgct ggaccgcggc aaccacaagc acctcgcgct
     1381 gacccaccag gcgacgaggc tcttccacgg ggccggctac acagcggtcg acggggaggt
     1441 cccccttgcg aactcgcacg cggcggtggc cgcgccgtac gcccatgtca ccgcgccgct
     1501 gcgccggctc gccgacaggt tcgggctcgt cgtgtccgag tgcgtgagca ggggcgccga
     1561 ggtgccggac tgggtccgcg cggcactgcc ggacctgccg gccgtgatgg cgatctcgga
     1621 caagcgggcc cacgccgtcg ggcgggcctg cgtcgacgcg gtcgaggcgg ccgtgctctc
     1681 ctcgcgggtc ggggagacgt tcgcggcctg ggtggtcgac gaggcgccta accacgaggt
     1741 ggcggtgcag ctcgccgacc ccgcgatcct cacgctcgcc tcgggcaagg ccggcctcgg
     1801 cgacgaggtg cgggtcgagc tgctcgaggc cgacccgctc accggtgtcg tccggatgaa
     1861 cctgcttggc tgaccggcaa gtagctgacc gggaaacagc agctgcgatg gcgccgccag
     1921 atcaacctcg atatcccacg gccgcctcgc gatgaccgcg acgaggcccc gccgccttgg
     1981 cccgccacag acccggtcac ccgagttccg gggcggccac tggcgatagc cttcgaccac
     2041 cggcccgccc ggtggcgatc ggtccaccat gcgtgacgcc gggcgacgtt cgaggaggcc
     2101 tgcaccggtg accgggacga aggagccgga cgcggaggcc gaccgcggcc cactgcaccg
     2161 tcgcccgtgg ctggcaagct atgcccccgg ggtcccgacc gatgtcgaca tccccaccgg
     2221 ttcgctgcac gagatgctcg cggacagcgc gcggcggtat gccggtcgcg ctgccctcga
     2281 cttcttcggg gcgcagacgt catacgcgga tctggccgac caggtgagcc tggcggcgga
     2341 ggccttgcac gccctgggcg tccggcacgg tgaccgggtg gcgctggtcc tgcccaactg
     2401 cccgcagcac atcgtcgcct tctacgcc
//
LOCUS       DATAIX010000243         1777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_162449, whole genome shotgun sequence.
ACCESSION   DATAIX010000243 DATAIX010000000
VERSION     DATAIX010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162449"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VHM65_03630"
     CDS             <1..398
                     /locus_tag="VHM65_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2704823.1"
                     /translation="TQKAMHAGLGSARCPGAVVNVASIAGLGATGVLGWYAVSKAALI
                     HLTTELGFQLAPRVRVNAVAPGVVKTRFAGALYEGREAEVAAAYPMKRLGVPDDIAGV
                     VSFLLSTEASWVTGQTLVVDGGVGLGGGL"
     gene            complement(424..1113)
                     /locus_tag="VHM65_03635"
     CDS             complement(424..1113)
                     /locus_tag="VHM65_03635"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEX2704824.1"
                     /translation="MVAAPVYYIADVPDFGPRLYREFHRTPVLGGDRIRTSLTQMFAG
                     SPADPDYSSPWPADTAVLSVATNGTTATVDLSGFVALGAAFESAAVQQLVYTVTAADP
                     SVRTVRLRVNGAVPPSGHSDWSAPIARDDPLDIQAHVWILGPAQGATVRSPVTVKVLG
                     TGWEGNVPLKVYRGSAVVAQGFVTTMMGGFAEAQRAFTLAPGSYEIRAYNDNGKDSSL
                     DLWDSKAFTVR"
     gene            complement(1335..>1777)
                     /locus_tag="VHM65_03640"
     CDS             complement(1335..>1777)
                     /locus_tag="VHM65_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704825.1"
                     /translation="VPVGATLGRFAQALGRTPTPMSAMEEATFTERRCAFRRLDGLPG
                     VAFMVIDDDPVGTVRVIGVGERSDMSTDTGIRLGDHLDEVRAIYGQGLNEPFDHYPVG
                     GQAVLVRADATSGVYWAFIGDQGDLVVDFRLGLKPEVLSPEGCS"
BASE COUNT          261 a          605 c          653 g          258 t
ORIGIN      
        1 ggacacagaa ggcgatgcac gccggacttg gctccgcccg ctgcccgggg gccgtggtca
       61 acgtcgcctc gatcgccggg ctcggcgcga ccggagtgct cggctggtat gccgtctcca
      121 aggcggcgct catccacctg acgaccgagc tcggcttcca gctcgcgccg cgggtccggg
      181 tcaacgcggt cgcgccgggc gtggtcaaga ctcgcttcgc cggtgcgctc tacgagggcc
      241 gcgaggccga ggtggcggcg gcatacccga tgaaacggct cggcgtcccc gatgacatcg
      301 cgggtgtcgt gtcgttcctg ctcagcacgg aggcaagctg ggtcaccggt cagacgctcg
      361 tcgtcgacgg cggcgtcggc ctcgggggcg ggctgtagcc ggccccgacc cgccggcgtc
      421 ggcctacctc acggtgaagg ccttgctgtc ccagaggtcc aggctcgagt ccttgccgtt
      481 gtcgttgtag gcccggatct cgtagctgcc gggtgcgagg gtgaaggccc gctgcgcctc
      541 ggcgaagccg cccatcatcg tcgtgacgaa gccctgggcg acgacggcgc tgccgcggta
      601 gaccttgagc gggacgttgc cctcccaccc cgtgccgagg accttgacgg tgaccgggga
      661 acggacggtc gcgccctgcg ccggaccgag gatccatacg tgcgcctgga tgtcgagcgg
      721 atcgtcccgg gcgatgggcg ccgaccagtc cgagtgcccg gagggcggca ctgccccgtt
      781 gacccgcagc cgtacggtgc ggaccgaagg gtcagccgct gtcaccgtgt agacgagctg
      841 ctggacggcg gcggactcga acgcggcccc gagcgcgacg aagccggaca ggtccacggt
      901 cgccgtcgtg ccgttcgtcg cgaccgacag cacggccgtg tccgcgggcc agggggagct
      961 gtagtcggga tcggccgggc tccccgcgaa catctgcgtc agcgaggtgc ggatccggtc
     1021 gcccccgagc acgggcgtcc ggtggaactc gcggtagagc cgcggcccga agtccggcac
     1081 gtccgcgatg tagtagacag gggcggcgac catcgccccg acaggagcgg gggatgacgt
     1141 ggtcgatgtc ggtggggggg cggacgagcc ggtcgtcgtg gtctgcgtca tcgtcgaacc
     1201 ggtcgtcgtt ggcgagccgg acgccgaccg gcccggcgtc cctgacggcc agagccacca
     1261 cacgccgagg cagagggtga ggacgaggac gctgaccaag acgggagcaa cccatcgggg
     1321 gcgggcaggt gcgttcatga gcagccctcc ggggagagaa cctcgggctt gagcccgagt
     1381 cggaaatcga ccaccaggtc cccctggtca ccaatgaagg cccagtagac acctgatgtg
     1441 gcgtcggctc gcaccaacac ggcctgtcct cccactgggt agtggtcgaa cggctcgttc
     1501 agtccctggc cgtagatcgc ccggacttcg tccaggtgat cgccgaggcg gatcccggtg
     1561 tcggtgctca tgtccgaacg ttcgccgacg ccgatcactc ggaccgtgcc cacgggatca
     1621 tcgtcgatca ccatgaaggc gacgccgggg agcccgtcga ggcgccggaa cgcgcaccgt
     1681 cgttcggtga aggtggcctc ttccatcgcg ctcatcggcg tcggtgtccg tcccagcgcc
     1741 tgggcgaacc tcccgagcgt ggcgccgacc ggaaccc
//
LOCUS       DATAIX010000244         1844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_214237, whole genome shotgun sequence.
ACCESSION   DATAIX010000244 DATAIX010000000
VERSION     DATAIX010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214237"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VHM65_03645"
     CDS             <1..544
                     /locus_tag="VHM65_03645"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2704826.1"
                     /translation="SLYFLSSLPTLKSAFFALIPASRRPRVIPLSEEVMRRVGSYAIG
                     QVAVAAVNAFFSWAMMLIVGIPYAAVLAVAVGFLGLVPMIGASLGAVCVGTVALFDEP
                     RKAIIAILYYIIYQQLENYAVMPRIMQRTVSVPGAVTVVAALAGGTLLGMLGALLAIP
                     AAAGFLLIYEEVIVPRQDHH"
     gene            565..>1844
                     /locus_tag="VHM65_03650"
     CDS             565..>1844
                     /locus_tag="VHM65_03650"
                     /inference="COORDINATES: protein motif:HMM:NF005869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB1 family IMP dehydrogenase-related protein"
                     /protein_id="HEX2704827.1"
                     /translation="MHFLEDRRPGHGLTYDDVFMVPRRSAVSSRMDVDLASIDGSGTT
                     IPLVVANMTAVSGRRMAETVARRGGLAVIPQDIPTEVVADVAAFVKSRDLVFDTPITF
                     GPQETVGTALNLLPKRAHGAAIVLDEARRPIGVVTEADLADRDRFTQLGEVMSRRLTT
                     LTEDVDPEAAFDRLGKARHRLAPVVDADGRLVGILTRPGALRATVYRPNTDASGRLRV
                     GAAVGVSREAAGKAVQLVAAGVDLLVIDTAHGHQEKMIETLRAVRALDLAVPIVAGNV
                     VSAAGVRDLVEAGADIVKVGVGPGAMCTTRMMTGVGRPQFSAVLECAAAARELGAHVW
                     ADGGVRHPRDVALALAAGAANVMVGSWFAGTLESPGDLHVGNDGRAYKESFGMASKRA
                     VRGRTVTDTSYERARKALFEEGISHARMYVDRDRP"
BASE COUNT          252 a          674 c          631 g          287 t
ORIGIN      
        1 cagcctctac ttcctcagct cgctcccgac gctcaagtct gccttcttcg ccctcatccc
       61 ggcgagtcgg cggccgcgcg tcatcccgct gtccgaggaa gtgatgcgcc gggtcgggag
      121 ctatgccatc ggccaggttg ccgtcgccgc ggtcaacgcg ttcttctcct gggcgatgat
      181 gctcatcgtc gggatcccgt atgccgccgt cctggccgtc gcggtgggct tcctcgggct
      241 cgtccccatg atcggggcgt cgctgggggc ggtctgcgtc ggcacggtcg cactcttcga
      301 cgagccgcgc aaggcgatca tcgccatcct ctactacatc atctaccagc agctcgagaa
      361 ctacgccgtg atgccccgca tcatgcagcg gaccgtgtcg gtccccggtg cggtgacggt
      421 cgtcgcggct ctggccggag gcactctcct ggggatgctg ggcgcgctgc tcgccatccc
      481 ggccgcggcg ggcttcctgc tcatctacga ggaagtcatc gtccctcgac aggatcatca
      541 ctgaggccac gtatcgtgtg ccgggtgcat ttcctcgagg accggcgacc cggccacggc
      601 ctgacctatg acgacgtctt catggtgccg cgccgctccg cggtgagcag ccgcatggat
      661 gtcgacctcg cgagcatcga cgggtcagga acgacgatcc cgctcgtggt tgccaacatg
      721 acggcggtct ccggccggcg gatggccgag accgtggccc gccgaggggg gctggccgtc
      781 ataccccagg acatcccgac cgaggtcgtc gccgacgtcg ccgcgttcgt caagagtcgc
      841 gacctcgtct tcgacacgcc gatcaccttc gggccgcagg agaccgtcgg gacggcgctg
      901 aacctgctgc ccaagcgcgc gcacggtgcg gccatcgtcc tcgacgaggc ccggcgcccg
      961 atcggggtcg tcaccgaagc cgacctcgcc gaccgcgacc ggttcaccca gctgggtgag
     1021 gtcatgtccc gccggctcac cacactgacc gaggacgtcg accccgaagc ggccttcgac
     1081 cgcctcggca aggcacgcca ccggctcgcc ccggtcgtcg acgccgacgg gcgcctggtc
     1141 gggattctca cccgcccggg tgcgctgcgc gcgacggtct accgcccgaa caccgacgcc
     1201 tccgggcggc tgcgggtcgg agcagcggtc ggggtcagcc gtgaggcggc gggcaaggcg
     1261 gtccagctcg tcgccgcggg ggtcgacctc ctcgtcatcg acaccgcgca cgggcaccag
     1321 gagaagatga tcgagaccct gcgggcggtg cgcgcgctgg acctcgccgt gccgatcgtg
     1381 gcgggcaacg tcgtgtccgc ggcaggggtc cgggacctcg tcgaagcggg ggccgacatc
     1441 gtcaaggtcg gggtcggccc gggtgccatg tgcaccaccc ggatgatgac cggtgtgggc
     1501 cgtccacagt tctccgcggt cctcgaatgc gccgcagccg caagggagct cggtgcccac
     1561 gtctgggccg acggcggcgt gcgccacccg cgcgacgtcg ccctcgccct ggccgccggc
     1621 gccgccaacg tcatggtcgg gtcgtggttc gccggcaccc tcgagtcccc cggggacctg
     1681 cacgtcggca acgacggacg cgcctacaag gagtccttcg gtatggcgtc caagcgcgcc
     1741 gtccgcggtc ggaccgtgac ggacacgtcc tacgagcgag cccgcaaggc gctcttcgaa
     1801 gagggcatct cgcacgcccg catgtatgtc gacagggacc ggcc
//
LOCUS       DATAIX010000245         4921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_13199, whole genome shotgun sequence.
ACCESSION   DATAIX010000245 DATAIX010000000
VERSION     DATAIX010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13199"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="VHM65_03655"
     CDS             complement(<1..1448)
                     /locus_tag="VHM65_03655"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX2704828.1"
                     /translation="MSRLLLLDGHSLAYRAFYALPAENFSTTTGQHTNAVYGFTSMLI
                     NVLRDEEPTHVAVAFDVSRQTFRATEYAEYKAGRSATPAEFSGQIPLLREVLDALSIP
                     YVEAPGYEADDVIATLTTSAVAAGMEVLICSGDRDAFSLVSNQVTVLYPIRGVSDLAR
                     MDPAAVTARYGVPPTRYSDLAALVGEASDNLPGVPGVGPKTAAKWISLYDDLPGIVAH
                     IDQISGKVGDALREHLDQVLRNRRLNQLVVDLDLGLGVADLERRTWDRDRVHTVFDSL
                     EFRVLRDRLFATLEVVRDEAESGFDVQTQVLASADVPGWLQAHAPPGSRVGVHVQGSW
                     ARGTGDAHGLAIAARPGAGEETGGAAAYLRVDELDEPAEQAVAAWLADPGRGKALHEA
                     KGPLQALAARGWTTLGVSSDTALAAYLVKPDQRSYDLSDLVLRTLHRELRADTEEGGQ
                     GLLDLGDQGESELAENAMVRARAVLELADALDT"
     gene            1466..1915
                     /locus_tag="VHM65_03660"
     CDS             1466..1915
                     /locus_tag="VHM65_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HEX2704829.1"
                     /translation="MDRQSPTGSTPPSQDVLAWMTSASAGTLAERMQIEYVELTPERL
                     VARMPVAGNTQPYGLLHGGASVVLAESLGSIGAILHAGEGRVAVGIEINATHHRAARE
                     GFVTGTARPVSVGRTLVCFEVVITDEQDRRLCTSRITCLLRDSPPGS"
     gene            1925..3373
                     /locus_tag="VHM65_03665"
     CDS             1925..3373
                     /locus_tag="VHM65_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX2704830.1"
                     /translation="MQPRRLRPFDASFLALDGPTTVGHVCLLSMLDGPVALADLRAQV
                     GARLHLVPPLRRRLAMLPLGLDRPWWVDDDAFDLDAHLSETTLPPGSDEATLADQVGA
                     IAETKLDRARPLWKLDVINGLPSGQAALVTTFHHCLVDGLGSRDLMGILFGADGTSPR
                     ADEDDDVADWRPGPLPSPWQMAFGTVRTVGDVTASAMRIQKRSISIVARSAGRVVGVA
                     AAMAGLEATGDGHVGRDGIAHRSPWITAPETPFNHALTSDRSCSFGVIPVPASARLRR
                     ATGTKVNDVMLAVTAGALRQWLLELGALPAAPLVALVPIGVETASGACEGNHLGLTLC
                     PLPTHLPDPRERLDRVHQSMLRAKTEPALTEALLTDLSFVSGPSVTTLFAEVAARVHL
                     ADRIRLPFNLMVSNVPSARQSWRVAGRIVTRNYPFPPLSDGLGLTIAVVGYGDVLGLG
                     LLACPVLVPDVWRLRDLLLAAHDELLDAYRWA"
     gene            complement(3406..3987)
                     /locus_tag="VHM65_03670"
     CDS             complement(3406..3987)
                     /locus_tag="VHM65_03670"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2704831.1"
                     /translation="MSRPHVVVRRADESLQGTVLDLWTAFRVESGSTGEAAARLVLES
                     RVGALLAREDVTALVAFADGEPVGYVLLTDSTGTEVLDGGGVTVDQLYVLKTARRHGV
                     ARQLLTAVIAYADRVGTDQLVTNVPTQGRDANRFFARLGFTPTVVRRVSTTAALRRRL
                     AGNESRYSVDQLLRRRRTARLRAAGSAAPAGRG"
     gene            4125..4898
                     /locus_tag="VHM65_03675"
     CDS             4125..4898
                     /locus_tag="VHM65_03675"
                     /inference="COORDINATES: protein motif:HMM:NF016365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEX2704832.1"
                     /translation="MRAAFPGLGTVINVMAVLLGSLSGMAIGHRLPGRTRAVVTDCLG
                     LTTLLMAGLSAAKVTDSSLGAALGSGAPVLVVLGSLLIGSVAGSLLQIERRLEGLAGV
                     IQSRFAARFGGTEAQGHAGRERFIEGWLSASLLFCVGPLTILGSLSDGLGRGIDQLAL
                     KSVLDGFAAMAFAASFGIGVLLSAVSVAVVQGLLTVAGVLLGTLLPDAHIAALTATGG
                     LMLVGIGLRLLRIKEVPVGDMLPALLVAPLLTQLVIAVR"
BASE COUNT          621 a         1830 c         1726 g          744 t
ORIGIN      
        1 gtgtcgagcg cgtcggccag ctcgagcacc gcccgggccc ggaccatcgc attctcggcg
       61 agctcgctct cgccctggtc gccgaggtcg agcaggccct gcccgccctc ctcggtatcg
      121 gcccgcagct cgcgatgcag ggtccgcagc acgaggtcgg acaggtcgta ggagcgctgg
      181 tcgggcttga cgaggtaggc cgccagcgcc gtgtcgctgc tgacccccag ggtcgtccac
      241 ccgcgggctg ccagcgcctg gagcggcccc ttggcctcgt gcagcgcctt gcccctgccc
      301 gggtcggcga gccacgcggc gaccgcctgc tcggccggct cgtcgagctc gtccacccgc
      361 aggtatgccg cggcgccgcc agtctcctcg cccgcgcccg gccgtgcggc gatcgccagc
      421 ccatgggcgt caccggtccc gcgggcccag ctgccctgga catggacgcc gacccgcgac
      481 ccgggcggag catgggcctg cagccaccca ggcacgtcgg cgctggccag cacctgcgtc
      541 tgcacgtcga agccggactc ggcctcgtcc cggacgactt cgagcgtcgc gaagagccgg
      601 tcgcgcagca cccggaactc caggctgtcg aagaccgtgt gcacccggtc acggtcccac
      661 gtgcgccgct cgaggtccgc cacgcccaga ccgaggtcga ggtcgacgac gagctggttg
      721 agccggcggt tgcgcaggac ctggtcgagg tgctcgcgca gggcgtcacc gaccttgccg
      781 ctgatctggt cgatgtgcgc gacgatgccc ggcaggtcgt catacaggct gatccacttg
      841 gccgccgtct tgggacccac gcccgggacc ccgggcaggt tgtcggacgc ctcaccgacc
      901 agggcggcga ggtcgctgta tcgcgtcggg ggcaccccgt agcgcgcggt cacggccgcc
      961 gggtccatcc gggcgaggtc ggagacccct ctgatcgggt agaggacggt gacctggttg
     1021 ctgacgagcg agaaggcatc gcggtccccg gagcagatga gcacctccat cccggcggcc
     1081 acggccgacg tggtcagtgt cgcgatgaca tcgtcggcct cgtagcccgg cgcctcgaca
     1141 tacgggatgc tcagcgcgtc gaggacctcg cgcagcagcg ggatctgtcc ggagaactcg
     1201 gccggcgtgg ccgagcggcc ggccttgtac tcggcatact cggtggcccg aaaggtctgc
     1261 cgtgagacgt cgaaggccac ggcgacgtgc gtcggctcct cgtcgcgcag gacgttgatg
     1321 agcatcgagg tgaacccgta gacggcgttc gtgtgctgtc ccgtcgtcgt cgagaagttc
     1381 tcggcaggca gcgcgtagaa cgcccggtag gccagggaat gaccgtcgag gagcagaagc
     1441 cgactcacag tggcagccta ggggcatgga ccgacagagc ccgaccggct cgaccccacc
     1501 gtcgcaggac gtcctcgcgt ggatgacgtc tgcgtcggcc gggaccctcg ccgagcggat
     1561 gcagatcgag tatgtcgagc tgacccctga gcgcctcgtg gccaggatgc cggtggccgg
     1621 gaacacccag ccctacgggc tgctccacgg cggtgcgagc gtcgtcctcg ccgaaagcct
     1681 cggctcgatc ggggccatcc tgcacgccgg tgaaggccgc gttgcggtcg ggatcgagat
     1741 caacgcgacc catcaccggg ccgcgcggga gggcttcgtc acggggaccg cccgtccggt
     1801 ctcggtcggg cgcaccctgg tctgcttcga ggtcgtcatc accgacgagc aggaccgccg
     1861 gctctgcacc tcccggatca cctgcctgct gcgtgacagc ccgcccgggt cctagcgaac
     1921 cgccatgcag ccgcgccgcc tccgaccgtt cgacgcctcg ttcctggccc tggacgggcc
     1981 taccaccgtg ggccacgtct gtctgctcag catgctcgat ggtccggtgg ccctggccga
     2041 tctgcgggcg caggtgggcg cgcggctgca cctcgtccct cccctgcggc ggcgcctggc
     2101 catgttgccc ctgggtctgg accgcccgtg gtgggtcgac gacgatgcct tcgacctcga
     2161 cgcgcacctg tccgagacca ccctgccccc cggcagtgac gaggccacgc ttgccgacca
     2221 ggtcggcgcg atcgccgaga ccaagctcga ccgcgcgcgc ccgctgtgga agctggacgt
     2281 catcaatggt ctaccgtcag gccaggcggc cctcgtgacc acgttccacc actgtctcgt
     2341 cgacgggctc ggctcccgcg atctcatggg catcctgttc ggtgccgacg ggacttcgcc
     2401 tcgcgcggac gaggacgacg acgtggccga ctggcgcccc ggtccgttgc cctccccctg
     2461 gcagatggcg ttcgggacgg ttcgcacggt cggggacgtc accgcctcag cgatgcggat
     2521 ccagaagcgc agcatcagca tcgtcgcccg ctcggccggt cgggtcgtcg gcgttgccgc
     2581 cgccatggcc ggcctggagg ccaccggcga cggacacgtc gggcgagacg gcatcgccca
     2641 ccgctcaccg tggatcaccg caccggagac gcccttcaac cacgccctca cctcggaccg
     2701 ctcgtgctcc ttcggcgtca tccccgtgcc tgcctcggcc cggctgcgcc gggccacggg
     2761 gaccaaggtc aacgacgtca tgctcgccgt cacggccggg gcgcttcgcc aatggctcct
     2821 cgagctcggc gcgctgcccg ccgccccgct cgtcgcgctc gtgccgatcg gtgtggagac
     2881 cgcgtcaggt gcctgcgaag gcaaccacct cggcctcacc ctgtgcccgc tgcccacgca
     2941 cctgcctgac ccccgcgagc gcctcgaccg ggtccaccag tcgatgcttc gcgccaagac
     3001 cgagcccgcc ctgaccgagg cgctgctcac cgatctctcc ttcgtgagcg gcccctcggt
     3061 cacgacgctc ttcgccgagg tcgcggccag ggtccacctc gccgaccgga tccggctgcc
     3121 gttcaacctc atggtgtcca acgtgccctc ggcccgccag tcctggcgcg tcgcgggccg
     3181 gatcgtcacg cggaactacc ccttcccgcc gctcagcgac ggtctcgggc tcacgatcgc
     3241 cgtcgtcggg tatggcgacg tcctcggcct cgggctgctc gcctgcccgg tgctcgtgcc
     3301 cgacgtctgg cggctgcgcg acctgctgct ggcggcccac gacgagctgc tcgacgccta
     3361 tcgctgggcc tgagctgcgt ctgagcctcc gagcgcaacg gcaggtcagc cgcgacccgc
     3421 cggtgctgcg gagccggccg cgcggagccg agcggtgcgc cgccgccgca ggagctggtc
     3481 gaccgagtag cgggactcgt tgccggccag ccggcgccgc agcgccgccg tcgtcgagac
     3541 ccggcgcacg acggtcgggg tgaacccgag ccgggcgaag aaccggttcg cgtcgcggcc
     3601 ctgggtgggc acgttggtga cgagctggtc ggtgcccacc cggtcggcat acgcgatgac
     3661 ggcggtgagc agctggcgcg ctaccccgtg gcgccgagcg gtcttgagga cgtagagctg
     3721 gtcgaccgtc acgccgccac cgtcgaggac ctcggtgccc gtggagtcgg tgagcaggac
     3781 atagcccacc ggctcgccgt cggcgaacgc caccaacgcc gtgacgtcct cgcgggcgag
     3841 cagcgcgccg acgcggctct cgagcaccag ccgggcggct gcctcgcccg tggagcccga
     3901 ctcgacccga aaggccgtcc acaggtcgag caccgtcccc tggagcgact cgtcggcgcg
     3961 tcgcacgacg acgtggggac gggacatacg ggcctccgac tcatcgcctg ggcgaacgga
     4021 actgggggcg agaccccctg gccccaactg cgactgtacg tccgaggcca cccctggcgg
     4081 aagagggaat ggtctaggcg cggacctgcc ctagggtggt tggggtgcgc gctgcctttc
     4141 cgggtctggg gaccgtgatc aacgtcatgg ccgtgctgct gggatccctg tccggtatgg
     4201 cgatcggtca ccgtctgccc ggccgcaccc gtgccgtggt gaccgactgc ctgggcctga
     4261 cgacgctgct tatggcgggt ctgtcggcgg ccaaggtgac ggactcctcc ctgggcgccg
     4321 ccctcggctc gggtgcgccc gtgctcgtcg tcctcggctc gctgctcatc gggtccgtcg
     4381 ccgggtccct gctgcagatc gagcgccggc tcgaggggct ggccggggtg atccagagcc
     4441 gcttcgcggc ccgattcggc ggcaccgagg cgcagggtca cgccgggcga gagcgcttca
     4501 tcgagggctg gctcagtgcc tcgctgctct tctgcgtcgg gccgctgacc attctcgggt
     4561 cgctctcgga cgggctcggg cgcgggatcg accagctcgc cctcaagtcg gtactcgacg
     4621 ggttcgccgc gatggccttc gcggcgtcct tcggcatcgg ggtgctgctc tcggccgtct
     4681 cggtggccgt cgtccagggc ctgctcacgg tcgccggggt gctcctcggc accctgctgc
     4741 cggacgctca catcgccgcg ctgaccgcca ccggcgggct catgctcgtc gggatcgggc
     4801 tgcgcctgct gcgcatcaag gaggtgccgg tcggcgacat gctgccggcc ctgctcgtcg
     4861 ctccgctgct cacccagctc gtcatcgccg tgcggtaggg cgtcgcggcg cgggcggggc
     4921 c
//
LOCUS       DATAIX010000246         7745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_162676, whole genome shotgun sequence.
ACCESSION   DATAIX010000246 DATAIX010000000
VERSION     DATAIX010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162676"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..1863
                     /locus_tag="VHM65_03680"
     CDS             58..1863
                     /locus_tag="VHM65_03680"
                     /inference="COORDINATES: protein motif:HMM:NF021588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-hydrolase family protein"
                     /protein_id="HEX2704833.1"
                     /translation="MDVSRVALVGRHPWWERQVGRGTPDSGRTALVDRVVGWRRDRIA
                     GVSGTGALVAVVAYVGSNTPSLLPRPWLLQGLVAALCAALGYVIGVGVGWLAHAFVEW
                     ADLRLTVRPSARRLMLTIWIALLGLGLLVAPLASLDWHRRIAEAVGFEAPGVGYVAAS
                     TAASVAGFLGLIGVWRLVAGLVDWVTVRVHRRILRETLARAVASLVTLVLVLLVVDQV
                     LVRGFLVVARRSAEQVNATQPRDFAAPTSSLRSGGPGSTEPWTSLGQDGAIFVSSGPS
                     QSDIVAATGRPAREPIRVYAGVGDGRSMEQTRDAAVAELERTGAFDRAAILVVTSTST
                     GFINEWSAAAFEYLGGGDTAVVSLQYSTLPSAVAFLTAAQDPPQAGRLLLDGVAARVT
                     QRPVGRRPKVYAAGESLGAYGGNGAFASPEDMLARVDGALWTGTPSFTDNARALASSR
                     ALGSTVVDPVVDNGAHVRFAGSPAELTADQFGRDLGSWRYPRVAYLQHPSDPVVWWST
                     DLIFATPPWLDVTRTASSPMAQMSWMPLVTFWQVTADLAMANSVPGGFGHRYFADETV
                     PAWAGILGLDPQADYSQIVSRINDATKTAEAGQLG"
     gene            complement(1900..2628)
                     /locus_tag="VHM65_03685"
     CDS             complement(1900..2628)
                     /locus_tag="VHM65_03685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX2704834.1"
                     /translation="MRDDTVAAVGRRPALATTLAVVAALVGLNVAGHLFGWESVWLGP
                     VGATALLAFARWSGLSWHQLGLGRGTYGRGLRWGAGVVGVVGAIYLGGVLIPATRTAF
                     LDVRYHMPASGALLTAFVVIPLGTVLLEEVAFRSVLWGVLSRHARAWQVLLTSSVLFG
                     LWHVFPAMAAVSNEVVGSALAGLGSLGPVVLVVGTVLFTACGGLVAGELRRRTGSVLA
                     AVGMHWATNALGVLFGVFAWHLAT"
     gene            2994..4397
                     /locus_tag="VHM65_03690"
     CDS             2994..4397
                     /locus_tag="VHM65_03690"
                     /inference="COORDINATES: protein motif:HMM:NF022432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HEX2704835.1"
                     /translation="MGKDNQARRRMKAKARRASRSQRSASPYDDSGQWSEERAWAPLS
                     DRDQLEYTVSALLTRVLVGWDERSPTEVDEGLTGLTALCSTPTGRRALVAAVLPMVRD
                     AITNAWRGGWQPLDVHRIVGRHSAAAEQRLLTDAIAHEMQAYAGSTVDPRWAAQLREL
                     DARVWWSRDTSYLLARHELGETWASILGGVLHVFHLVAGLPRMERLIPIPGTASAGTS
                     ARPAPDAPVIDERILRRVRALLAQAESTTYPAEAETFTAGAQALMARHSIDAALLAAD
                     QPRHDAPHARRIGLDNPYEGPKAMLLDAVASANRCRTVWSRELGFSTVMGFGPDLDAV
                     EVVFTSLLVQATHALTAQGTRTDRFGRSRTRAFRQSFLTAYATRIGERLREVTREQVD
                     AAAHDVGRSSGRALVPLLHARSAEVDAAVDAMFPDLVTRRLGSVTDNEGWHSGRHAAD
                     LARLGGHPGLDQLPGVG"
     gene            4537..5808
                     /locus_tag="VHM65_03695"
     CDS             4537..5808
                     /locus_tag="VHM65_03695"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX2704836.1"
                     /translation="MSEKRGVAMAGALSGLRVVEGSAFVAMPLGGMTLAQLGADVIRF
                     DPIGGGLDYTRWPVTLDGRHSLFWAGFNKGKRSIAVDIRAPEGQEILTRLITAPGPDA
                     GIFLTNFPARGWTAYETLRSHREDLIMVHLVGRRDGGSEVDYTVNPQTGLPFVTGPTD
                     FHRPVNHLLPAWDCIAGQMAVVGLLAAERHRRLSGEGQLVRLALKDVALAMLGHLGKI
                     AEVMVNDADRPKDGNYLYGAFGRDFGTLDGKRLMVVALTLGQWVNLVRVTQLDEELVA
                     LAKRLRLDFAKEGDRFRARREIARVFEPWFATKLLAEIRHQFDTAGVTWAPYRTVREA
                     VEHDPDCSTDNPMFALVEQPEVGTFLMPGLPIDFGACERAPVERAPKLGEHTDEILMD
                     VLGMDSAQVGRLHDRAVVAGPEESAFSHLVD"
     gene            5842..6729
                     /locus_tag="VHM65_03700"
     CDS             5842..6729
                     /locus_tag="VHM65_03700"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX2704837.1"
                     /translation="MSAMTAACAASEREHPRSAAATAVTWLFVPGERPERFDKAAGSA
                     ADEVVLDLEDAVPPAGKDAAREQVCRWLASAGTGWVRVNGADTPWFEADLAALAAVGA
                     VPALAARTGLRGVLMPKADDPEVLALVRARVGPDRGVMALVESARGVAGALALAATDA
                     VDRLAFGSVDLAVDLDAVEEDRSLLLARSMLVLASRAAGKPGPVDGITGSWDDPELVR
                     SDAYRSRRLGFTGKLCIHPAQLDPAAAAFRPTEAELTWAQRVLDALGDDAPGAVAVAG
                     QMVDKPVLDRARAIARRAR"
     gene            6779..7681
                     /locus_tag="VHM65_03705"
     CDS             6779..7681
                     /locus_tag="VHM65_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2704838.1"
                     /translation="MTDILERTEVLAPAPGLALAGLLGVQAPDLAADEPLPIPWHWVY
                     LLERPAQADLGPDGHPARGGIPVPPGPGRRRMWASGTVTGLGPLRCGRPATRRTQVLG
                     SQDKEGRSGPMTFVTVGHEIVQDGAVVVREEQHIVYLDAAPPVGGAAGAASGGSSGMP
                     QAPAVTPDPSVPSVPSVPAVPIGPAEWDVVVDPTLLFRYSALTYNAHRIHYDRDFARD
                     VEGYPGLVVHGPLQATLMAEAARRRGLVEPGRPVQLAYRMQSPLFDHQGLVVSAVPGP
                     DGVATSVRDRWGRVTATGTLRLPS"
BASE COUNT         1070 a         2823 c         2788 g         1064 t
ORIGIN      
        1 caccccggcg accgggtcca caccaccgac ggggctccgg cgtgagagtt tacaggggtg
       61 gacgtttccc gcgtggccct cgtcggtcgc cacccctggt gggaacgcca ggtcgggcgc
      121 ggcacgcccg actccgggcg cacggccctc gtcgaccggg tcgttgggtg gcgccgagac
      181 cgaatcgctg gggtctcggg caccggggcg ctcgtggcgg tggtggctta cgtcgggtcg
      241 aacaccccct ccctgctgcc gcggccgtgg ctgctccaag gcctcgtcgc ggccttgtgc
      301 gccgcgctcg gctacgtcat cggcgtgggg gtgggctggc tggcccacgc gttcgtcgag
      361 tgggccgatc tgcgcctgac ggtccgaccg tcggcacgcc gactgatgct gacgatctgg
      421 atcgccctgc tgggcctcgg actgctcgtc gcgcctctgg cgagcctcga ctggcaccgc
      481 cgcatcgccg aggcggtggg gttcgaggcg ccgggggtcg ggtacgtcgc tgcgagcacc
      541 gctgcctcgg tggcggggtt cctcgggttg atcggcgtgt ggcggctggt cgccgggttg
      601 gtcgactggg tcaccgtccg cgtgcaccga cggatcctgc gcgagacgtt ggcgcgcgca
      661 gtggcaagcc tcgtcaccct cgtgctggtt ctgctcgtcg tggaccaggt gctcgtgcgc
      721 ggcttcctcg tcgtagctcg gcgcagcgct gagcaggtca acgccaccca gccgcgcgac
      781 ttcgctgcgc cgacctcatc gctgcgctcg ggcggcccag gctccaccga gccgtggacc
      841 agcctcggtc aggacggtgc gatcttcgtc tccagcgggc cgagccagtc cgacatcgtg
      901 gcggcgaccg gccggcccgc ccgggagccg atccgcgtct acgcgggcgt cggcgacgga
      961 cggtcgatgg agcagacccg cgacgcggcc gtcgccgagc tcgagcggac cggggccttc
     1021 gaccgggcgg ccatcctcgt ggtcacctcg acgagcaccg gcttcatcaa cgagtggagc
     1081 gcggctgcct tcgagtacct cggcggcggc gacaccgccg tcgtgtcgtt gcagtattcg
     1141 accctgccct cggccgtcgc cttcctcacc gcggcccagg acccgcccca ggcgggccga
     1201 ctgctcctgg acggggtggc ggctcgggtg acacagcgcc ccgtcgggcg ccgtcccaag
     1261 gtctatgccg cgggcgagtc gctcggtgcc tacggcggga acggcgcgtt cgcctctccg
     1321 gaggacatgc tcgcccgcgt cgacggagcc ctgtggaccg ggacgccgtc cttcaccgac
     1381 aacgcccggg cgctcgcctc gagtcgggcc ctcggctcca cggtggtcga cccggtcgtc
     1441 gacaacggcg cacacgtccg gtttgccggc tcacccgccg agctcaccgc cgaccagttc
     1501 ggccgtgacc tcggctcgtg gagatacccg cgcgtcgcct acctgcagca cccgtcggac
     1561 cccgtcgtgt ggtggtcgac tgacctgatc ttcgccacgc cgccgtggct cgacgtgacc
     1621 cgcacggctt cctcgccgat ggcccagatg tcctggatgc ccctggtgac cttctggcag
     1681 gtcacggcgg acctggccat ggccaacagt gtcccgggcg gtttcggcca ccgctacttc
     1741 gccgacgaga ccgtcccagc gtgggcgggg atcctgggtc tggacccgca ggccgactac
     1801 tcgcagatcg tcagccggat caacgacgcg acgaagaccg ccgaggcggg ccagctgggc
     1861 tgagcggccg ctccacgtcc tgggcccagg cgagcaccgc tacgtcgcca gatgccaggc
     1921 gaagaccccg aacaggaccc ccagggcgtt ggtggcccag tgcatcccca ccgcggccag
     1981 gacactgccg gtacggcgac gaagctcccc ggcgaccagg cctccgcagg cggtgaacag
     2041 caccgtgccg acgaccaaca cgaccgggcc aagactgccc aagccggcca gcgcggaccc
     2101 gacgacctcg ttggagaccg cggccatggc cgggaagacg tgccagaggc cgaagaggac
     2161 ggaggaggtg agcagcacct gccaggcgcg ggcgtgccgc gagagcaccc cccacagcac
     2221 cgagcggaac gccacctctt ccagcagcac cgtgccgagc gggatcacga cgaacgcggt
     2281 gagcagcgcg ccggaggcgg gcatgtggta gcgcacgtcg aggaaggcag tgcgggtggc
     2341 cgggatgagc actccgccca ggtagatcgc gccgacgact ccgacgaccc cagcgcccca
     2401 gcgcagtccc cggccgtagg tgccgcgtcc caggccaagc tggtgccagg agagccccga
     2461 ccagcgggcg aaggccagca gggcggtggc tccgaccggg cccagccaca ccgactccca
     2521 gccgaagaga tggccggcca cgttgagccc gaccagtgcg gccacgacgg cgagggtggt
     2581 agccagggca gggcggcgcc cgacggcggc cactgtgtcg tctcgcatcc cgaccattgt
     2641 cacacgcagc gcgacccgct cactcacacc tcggtagggc gcacgtgcca ggccgctcga
     2701 cctcgtcgcc gtactcgatc ctggacttga tgccgccgtg caggatgccg accaaccggc
     2761 gagctgctgt cggcacccct cgttcacagg tgagcgaggc cccaggccac aactccgata
     2821 ccgttgagcc gaggcaaccc catgaacgac tccagagtcc gtgatctccc atgtcaacac
     2881 acgtcacgcg gccatcacca gcgcgcgtcg cctgcgcgcc gggcggccga cctggcactc
     2941 tggacggcat accgacgagg aacgcatacc gacgaggaac ggacagtcac cgcatgggca
     3001 aggacaatca ggcgcgccgg cgcatgaagg ccaaggccag acgagccagc cgcagtcagc
     3061 ggtcggcgtc accctacgac gacagcgggc agtggagcga ggaacgcgcc tgggcgccgc
     3121 tgagcgatcg cgaccagctc gagtacaccg tgagcgcgct gctcacgcgc gtcctcgtcg
     3181 gctgggacga gcgctccccc accgaggtcg acgaggggct gaccgggctc accgcgctct
     3241 gttcgacccc gaccgggcgc cgggccctcg tcgcggccgt cctgcccatg gtccgcgacg
     3301 cgatcaccaa cgcgtggcgc gggggctggc agcctctaga cgtgcaccgc atcgtcggac
     3361 gccacagcgc ggcggccgag caacggctgc tcaccgacgc catcgcccac gaaatgcagg
     3421 cgtatgccgg ctcgaccgtg gacccgcggt gggcggcaca actgcgcgaa ctggacgcgc
     3481 gggtctggtg gtcccgggac accagctatc tcctagcgcg ccatgagctc ggcgagacct
     3541 gggcgtcgat cctcggcggc gtgctgcacg tgttccatct ggtggccggc ctgcctcgga
     3601 tggaacgact catccccatc ccgggcacgg cgagcgccgg cacgagcgcc cgtccggcac
     3661 ccgatgctcc ggtcatcgac gagcggattc tgcgccgcgt ccgggccttg ctcgcgcagg
     3721 cggaatcgac gacctacccg gccgaggcgg agacgttcac ggccggcgcg caggccctca
     3781 tggcccggca cagcatcgac gccgcgttgc tcgccgccga ccagccacgc cacgacgcgc
     3841 cgcacgcgcg tcggatcggc ctggacaacc cctacgaagg ccccaaggcc atgctcctgg
     3901 acgccgtcgc ctcggccaac cggtgtcgca ccgtgtggag ccgtgagctc ggcttcagca
     3961 ccgtgatggg gttcggcccc gacctcgatg ccgtcgaggt ggtcttcacg tcgctgctgg
     4021 tgcaggcgac ccatgcgctg acggcccagg gcactcggac cgaccggttc gggcgcagcc
     4081 gcacgcgagc cttccgtcag tccttcctca cggcatacgc gacccggatc ggggagcggc
     4141 tgcgcgaggt gactcgcgag caagtcgacg ccgccgcaca cgacgtcggc cgctcgagcg
     4201 gccgggcgct cgtgccgctg ctgcacgccc gatcggccga ggtggatgcc gccgtcgacg
     4261 cgatgttccc ggacctcgtg acgcgcaggc tcgggtccgt gactgacaac gaagggtggc
     4321 actccggtcg gcacgccgcg gacctcgcgc gtctgggcgg ccaccctggt ctcgaccagc
     4381 tgccgggcgt cgggtagcca cgccagcgcc ggggccgtgt ccgggtcgcc gacgggacca
     4441 ccgttggccg gccgtacgga gtgaggctgc tcacggggcc tcctcggtgg ctggtcccct
     4501 gttgccgacc gccggtgatt gcgccactgt ggagacttga gtgagaaaag gggcgtcgcc
     4561 atggcaggag ccctctctgg tcttcgggtt gtcgaggggt cggccttcgt ggccatgccg
     4621 ctgggcggca tgaccctggc ccagctcggc gccgacgtga tccggttcga ccccatcggc
     4681 gggggtctgg actacaccag gtggccggtc accctcgacg ggcggcacag cctgttctgg
     4741 gccgggttca acaagggcaa gcggtcgatc gccgtcgaca tccgcgcccc cgaggggcag
     4801 gagatcctca cccggctgat caccgccccc ggaccggacg ccgggatctt cctcaccaac
     4861 ttcccagcgc gaggctggac ggcatacgag accctccgca gccaccgcga ggacctcatc
     4921 atggtccacc tggtcgggcg gcgggacggc ggctccgagg tggactacac ggtcaacccg
     4981 cagaccgggt tgccgttcgt caccggaccg accgacttcc accgcccggt caaccacctc
     5041 ctgccggcgt gggactgcat cgcgggtcag atggccgtcg tcggcctgct cgccgccgag
     5101 cggcaccgcc ggctgagcgg cgaggggcag ctggtgcggc tcgcgctcaa ggacgtcgcg
     5161 ctggcgatgc tcggccacct cggcaagatc gccgaggtga tggtcaacga cgccgaccgg
     5221 cccaaggacg gcaactacct ctacggggcg ttcggtcgcg acttcggcac cctggacggc
     5281 aagcggctca tggtcgtcgc gctgaccctc ggccaatggg tcaacctggt ccgcgtcacc
     5341 cagctcgacg aggagctggt ggcgctggcg aaacggctgc ggctcgactt cgccaaggag
     5401 ggcgaccggt tccgggcccg ccgggagatc gcccgggtct tcgagccgtg gttcgcgacg
     5461 aagctgctcg ccgagatccg ccaccagttc gacaccgccg gggtcacctg ggcgccctac
     5521 cgaaccgtcc gggaggccgt cgagcacgac ccggactgct ccaccgacaa cccgatgttc
     5581 gccctggtcg agcaaccgga ggtcgggacg ttcctcatgc ccgggctgcc gatcgacttc
     5641 ggcgcctgcg agcgcgcacc ggtcgagcgg gccccgaaac tcggcgagca caccgacgag
     5701 atactcatgg acgtgctcgg gatggacagc gcgcaggtcg ggcggctgca cgaccgggcc
     5761 gtggtcgccg gtcccgagga gtccgccttc agccacctgg tcgactgacg acggcccacg
     5821 atccccggac gaaagggccc gatgagtgcg atgactgcag cgtgtgcggc cagtgagcgt
     5881 gagcacccaa ggtcggccgc ggccaccgcg gtgacctggt tgttcgtgcc cggcgagcgg
     5941 cccgagcggt tcgacaaggc ggccggctcc gctgccgacg aggtcgtcct ggacctcgag
     6001 gacgcggtgc cccccgccgg caaggacgcg gcccgcgagc aggtatgccg gtggctcgcc
     6061 agcgccggga ccggctgggt ccgggtcaac ggcgccgaca cgccgtggtt cgaggccgac
     6121 ctggcggccc tggcggcggt gggggccgtg cccgctctgg ctgctcggac cgggctgcgg
     6181 ggagtgctca tgccgaaggc ggatgacccc gaggtgctcg cgctggtgcg cgcgcgggtc
     6241 ggtccggacc ggggcgtgat ggccctggtc gagtcggcgc ggggggtggc cggggcgctc
     6301 gcgctggccg ccacggacgc ggtggaccgg ctggctttcg gctcggtgga cctggcggtc
     6361 gacctcgacg cggtcgagga ggaccgctcg ctgctgctgg cccgctcgat gctcgtcctc
     6421 gcctcccggg ccgccggaaa gcccggacca gtcgacggga tcacggggag ctgggacgac
     6481 cccgagctgg tccgctcgga cgcctaccgc tcccggcggc tggggttcac cggcaagctg
     6541 tgcattcacc ccgctcagct cgatccggcc gccgcggcgt tccggcccac cgaggccgag
     6601 ctgacctggg cgcagcgggt gctcgatgct ttgggcgacg acgccccggg agcggtcgcg
     6661 gtcgccggcc agatggtcga caagcccgtg ctcgaccggg cccgggcgat cgcgcggcgg
     6721 gcacggtgac acgggtgccc ggcatacata gtcgtggttg tcacggggag gaggtgcgat
     6781 gaccgacatc ctggaacgca ccgaggtcct cgcgccggcg cctggtctgg cgctggccgg
     6841 gctgctcggc gtgcaggcgc cggatctggc ggccgacgag ccgctgccga tcccctggca
     6901 ctgggtctat ctgctcgaac ggccggctca ggccgacctg ggccccgacg gacaccccgc
     6961 ccgcggcggg atccccgtgc cgccgggtcc ggggcggcgg cggatgtggg cgagtggcac
     7021 cgtgaccggg ctgggcccgc tgcgctgcgg acggccggcc acccggcgga cccaggtgct
     7081 cggctcgcag gacaaggagg gccgctccgg gccgatgacc ttcgtgacgg tcgggcacga
     7141 gatcgtccag gacggtgcgg tcgtcgtccg ggaggagcag cacatcgtct acctggacgc
     7201 ggcgccgccg gtcggtgggg cagcgggcgc ggcctccggg ggctcttctg gtatgccgca
     7261 ggccccggcc gtgacgccgg acccgtcggt gccgtcggtg ccgtcggtgc cggcggtccc
     7321 gatcggtcct gccgaatggg acgtggtcgt cgacccgaca ctcctgttcc gctactccgc
     7381 gctgacctac aacgcgcacc gcattcacta cgaccgggac ttcgcccgcg acgtcgaggg
     7441 ctacccggga ctggtcgtcc acgggccgct gcaggccacc ctcatggccg aggcggctcg
     7501 gcggcgcggg ctggtcgagc ctggccggcc ggtgcagctc gcctaccgca tgcagtcgcc
     7561 gctgttcgac caccagggcc tggtcgtctc ggcggtcccg ggaccggacg gcgtcgccac
     7621 ctcggtccgc gaccgttggg gccgggtcac cgccacgggg acgctccgcc tgccctcgta
     7681 gcccacgttg accagatcga taggtgatcg aaaccaccgg ggcattgttg agacctggcc
     7741 cacac
//
LOCUS       DATAIX010000247         4411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_214275, whole genome shotgun sequence.
ACCESSION   DATAIX010000247 DATAIX010000000
VERSION     DATAIX010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214275"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..2347
                     /locus_tag="VHM65_03710"
     CDS             17..2347
                     /locus_tag="VHM65_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704839.1"
                     /translation="MLSLEPPYPVIGGYALLPDHADPLLYYAMPAGPRLAMLDTGPAL
                     NLTLYADSGAGAARTSGGILSMTTELVVPGDVLAALPGQLASVRGITGTPRVVPVSFE
                     SGTVELAVLGRTSTPPAQGSAAPGGLFELTFVAGGRPSLTGTNDAAFQLVLGESAAEA
                     IEACLDEGAQPVLVTYTMTYAALRPAFTVDLDVDWTKIYHSLQSKLTTNAWFVAADVE
                     ANVKSAFEESGIRIDTVVEGTGSGDRAAAERTQAQLLDWVMDRLFEPMIPAVSTAESI
                     GQVVEGALWSLARAVIPGASYRLKVVDETQVRRMNIRARERVAELREVRPQGSIDALL
                     RSLRTTADGTPSPHWPAIRAAMVQRVELDGFPRLEVGVSVVDRFAADGLRSVDVEMAR
                     APAGGGDPPPGDVTALGFTPGTTAQSWVVNLLDEAAREAVWKRPYVYRVVARFDPSSP
                     LRPGEVVTSAWTDRRTSDLLVDPREAYAVTDVSVTVAPLFSFELFPAVTVDLHPGTPG
                     GPATALAGTRRVQLDAEHPLQHWVFTSLPGDLSYSYRATYHRPSEAGGAVTTEWGAAV
                     EPFLSLPDPMPEKLTVTFFVDLPWTELSLAMLQIRYDDAAHDVHYAQETLTLEAGTTH
                     VARTYSIASGGSRSLSYRLTLQRAAGGLVDGSWREAGDDRVVVDRRLVDERAVRVKAL
                     GTLADRRLVRAAVRIEARDPAAGLVRAATDVVIPGGGEGNPIAPFTYLLGDPPLHEVW
                     WQLTTVDSNGFPAAQPWGHGTSDLLVVDLRTLSVTG"
     gene            2355..>4411
                     /locus_tag="VHM65_03715"
     CDS             2355..>4411
                     /locus_tag="VHM65_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704840.1"
                     /translation="MLSLEQPPLSVGGLTVFADHADGGQFYYAAPHPRLARSGGQPML
                     DLFTYAVELTSSPLAGTRIPDELGAGFLTMGVDCALAEGARGSALQALATRLGRDPGQ
                     IALSPIPYLRGTVSVIALDAASGAAAASMPAVVGDRPRFVEAVVGTGAPSLIGDLRSI
                     FSLSLSQEGVTFLEGLYADAAAPVGVVYNLTFLGLRPSVQAHVHADVSKIYTELGGKA
                     SVGCAYVRAEVEGLLTTLEQRGVVTVELTTQAVGPEATAAKELALSLFKDRIIQELFN
                     PAPQLPNIAMPTIPGAGQTSIVTLSLKAKRDEELRTVDYDFSERSPEERTHAPQAFIA
                     SLLSAEEMAARIHHVDLASDFFELLEVLVSGPSAEEFAALGLRSVTIDLRYGGEEAGS
                     GIPADAGSGIPADADSLVFRPGSPSDLTWAVRRKGRHTLGYTAAVTYEFNREGSVDAD
                     ALTYRIPPRPETGRTLSVRPYDDLAILDVEVDLGRLDPGVRDVDVTLAYDDPATGFTA
                     QHQLRLSPATPLPLEQRRWQVRTTTAGTRAYTAHSVLTFTDGAVFALPPVTTSEHLAR
                     IDAPFRASRSLLVQPNVTSDDVTSMTVEVRYDDAAAGYARRFTQVLTPSPALAPAPAD
                     PTVPASAAGQTPAPTPPAASPGSRWAPVTLSWPILDAARQEIQYRVTTAAGGVIDAGE
                     WTPT"
BASE COUNT          587 a         1734 c         1466 g          624 t
ORIGIN      
        1 accctaggga ccggccgtgc tctcgttgga gccgccctac ccggtcatcg gcgggtacgc
       61 gctcctgccc gaccatgccg acccgctgct ctactacgcc atgccggcgg ggccgcgcct
      121 cgccatgctc gacaccggcc ccgcgctgaa cctcacgctg tatgccgaca gcggtgcggg
      181 cgcggcgcgc accagcgggg gcatcctgtc gatgacgacc gagctcgtcg tccccggcga
      241 cgtcctggcg gccctgcccg gacagctcgc gagcgtgcgg ggcatcaccg ggacgccccg
      301 ggtcgtgccg gtgagcttcg agtcggggac ggtcgaactc gccgtgctcg ggcgcacctc
      361 gacacccccg gcgcagggct cagcggcacc cggcggactg ttcgagctca ccttcgtcgc
      421 gggcggcagg ccgtcactga ccgggaccaa cgacgccgcc ttccagctcg tcctcggcga
      481 gtcggccgcc gaggccatcg aggcctgcct cgacgagggc gcccagcctg tcctggtgac
      541 ctacaccatg acgtatgccg ccctgcggcc ggcgttcacg gtcgacctcg acgtcgactg
      601 gaccaagatc taccactcgc tgcagagcaa gctgacgacc aacgcgtggt tcgtcgcggc
      661 cgatgtcgag gcgaacgtca agagcgcctt cgaggagtcc gggatccgca tcgacacggt
      721 cgtcgagggc accgggtccg gtgaccgggc tgcagcggag cgcacccagg cccagctgct
      781 cgactgggtc atggaccggc tgttcgagcc gatgatccct gcggtgagca cggccgagtc
      841 catcggtcag gtcgtcgagg gggccctctg gtccctcgcc cgcgccgtca tcccgggcgc
      901 gtcataccgg ctcaaggtcg tcgacgagac ccaggtacgc cggatgaaca tccgggcccg
      961 cgagcgggtg gccgagctgc gcgaggtacg cccgcagggc tcgatcgacg ccctgctgcg
     1021 ctcactgcgg accacggcgg acgggacgcc ttcaccgcac tggccggcga tccgcgccgc
     1081 catggtccag cgcgtcgagc tggacggctt cccgcggctc gaggtcggcg tctcggtcgt
     1141 cgaccggttc gccgccgacg gcctgcgcag cgtcgacgtc gagatggccc gcgcccccgc
     1201 cggcgggggc gacccgccac ccggcgacgt caccgccctc ggcttcacgc cgggcacgac
     1261 cgcccagtcg tgggtcgtca acctgctcga cgaggcggcc cgcgaggcgg tgtggaagcg
     1321 cccctatgtc taccgggtgg tcgcccgatt cgacccgtcc tcgccgctgc ggcccggcga
     1381 ggtcgtcacg tcggcgtgga ccgaccggcg cacgtccgac ctgctcgtcg acccgcgcga
     1441 ggcgtatgcc gtcaccgacg tcagcgtcac ggtggcgccg cttttctcct tcgagctgtt
     1501 ccccgccgtc accgtggacc tgcaccccgg cacgcccggc ggcccggcga ccgccctcgc
     1561 aggcacccgc cgggtccagc tcgacgccga acacccgctc cagcattggg tgttcacgtc
     1621 cctgcccggt gacctgtcct actcctaccg ggcgacctac caccgtccct ccgaggccgg
     1681 cggggccgtg acgaccgagt ggggagcggc cgtcgagccc ttcctgtcgc tgcccgaccc
     1741 gatgcccgag aagctcaccg tgacgttctt cgtcgacctg ccgtggaccg agctctccct
     1801 cgccatgctc cagatccggt atgacgacgc ggctcacgac gtccactacg cccaggagac
     1861 gctgaccctc gaggcgggga cgacccacgt ggcgcggacg tactcgattg cctccggcgg
     1921 atcccggtcg ctgtcctacc ggctcaccct gcagcgcgcc gccgggggcc tggtcgacgg
     1981 gtcgtggcgc gaggccggcg acgaccgggt cgtcgtcgac cgccgcctcg tcgacgagcg
     2041 agcggtccgg gtcaaggcac tcggcacgct cgccgaccga cggctcgtgc gagccgccgt
     2101 gcggatcgag gccagggacc ccgcagcggg cctggtgcgc gcggcgacgg atgtcgtcat
     2161 acccggtggt ggtgaaggga acccgatcgc ccccttcacg tatctgctcg gcgacccgcc
     2221 gctccacgag gtgtggtggc agctgactac ggtcgacagc aacgggttcc ccgccgcgca
     2281 gccgtggggg cacgggacct cggacctgct ggtcgtcgac ctgcgcacgc tgagcgtgac
     2341 ggggtgagcg gcccatgctg agcctcgaac agccaccgct gtccgtcggc gggctcaccg
     2401 tcttcgccga ccacgccgat ggcgggcagt tctactacgc cgcgccgcac ccccgtctcg
     2461 cgcgcagcgg cggccagccg atgctcgacc tgttcaccta tgccgtcgag ctgacgtcct
     2521 cgccgctggc cgggacgcgg atccccgacg agctcggcgc cgggttcctc acgatgggcg
     2581 tcgactgcgc gcttgccgag ggtgcccgcg ggtccgccct ccaggcgctg gccacgcgcc
     2641 tcggccgcga ccccggccag atcgcgctgt cgccgatccc ctacctccgc ggcacggtct
     2701 ccgtcatcgc cctcgacgcc gcgtcgggcg ccgcagccgc ctcgatgccc gccgttgtcg
     2761 gcgatcggcc acggttcgtc gaggccgtcg tcgggacggg ggcgccctct ctcatcggtg
     2821 acctgcgctc gatcttctcg ctgagcctga gccaggaggg ggtcaccttc ctcgagggcc
     2881 tgtatgccga cgccgccgcc ccggtcgggg tcgtctacaa cctcaccttc ctcggcctgc
     2941 gcccgagcgt ccaggcccac gtgcacgcag acgtctcgaa gatctacacc gagctcggtg
     3001 gcaaggccag cgtcgggtgc gcctacgtca gggccgaggt cgagggcctg ctcaccaccc
     3061 tcgagcaacg cggggtcgtc acggtcgagc tgacgacgca ggccgtcggt cccgaggcca
     3121 cggccgccaa ggagctggcc ctgtccctgt tcaaggaccg gatcatccag gagctgttca
     3181 acccggcccc ccagctgccg aacatcgcca tgccgaccat ccccggcgcc gggcagacct
     3241 cgatcgtcac gctgagcctc aaggccaaac gcgacgagga gctgcgcacg gtcgactacg
     3301 acttctccga gcgctccccc gaagagcgca cgcacgcccc gcaggccttc atcgcctcgc
     3361 tgctcagcgc cgaggagatg gccgcgcgga tccaccacgt cgacctcgcc agtgacttct
     3421 tcgagctgct cgaggtgctc gtgtccggcc ccagcgccga ggagttcgcc gccctcgggc
     3481 tgcgctcggt gaccatcgac cttcgctacg gcggcgagga ggcgggctcc ggcatacccg
     3541 ctgacgcggg ctccggcata ccggctgacg cggactcgct ggtcttccgc cctggttcgc
     3601 cctccgacct cacctgggcc gtgcgccgca agggccggca tacgctcggc tacacggcgg
     3661 cggtgaccta cgagttcaac cgcgaagggt cggtcgacgc cgacgccctc acctaccgga
     3721 tccccccgcg ccccgagacg ggccgcacgc tctcggtccg cccctacgac gacctcgcga
     3781 tcctcgacgt cgaggtcgac ctcggccgcc tcgacccggg agtgcgcgat gtcgacgtca
     3841 cgctcgcgta tgacgacccc gccaccggct tcacggcgca gcaccagctg cggctcagtc
     3901 ccgcgacgcc gttgcccctc gagcagcgcc ggtggcaggt ccgcacgacg acggccggga
     3961 cccgggccta caccgcccac agcgtcctca ccttcaccga cggggcggtc ttcgcgctcc
     4021 cgccggtcac gacgagcgag cacctcgcgc ggatcgacgc gccgttccgc gcgagccgtt
     4081 cgctgctcgt ccagcccaac gtgaccagcg acgacgtcac ctcgatgacc gtcgaggtcc
     4141 ggtatgacga cgccgccgcc ggctatgcgc gccgcttcac ccaggtcctc accccgtcac
     4201 cggctctggc accggccccc gccgacccca cggtgcccgc gtcggcggcg gggcaaaccc
     4261 cagcaccaac accacccgcg gcttccccgg ggagccgatg ggcgccggtg acgctgagct
     4321 ggccgatcct cgacgccgct cgccaggaga tccagtaccg cgtgacgacc gccgccggtg
     4381 gcgtcatcga cgccggggag tggactccca c
//
LOCUS       DATAIX010000248         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_103004, whole genome shotgun sequence.
ACCESSION   DATAIX010000248 DATAIX010000000
VERSION     DATAIX010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103004"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..1136
                     /locus_tag="VHM65_03720"
     CDS             333..1136
                     /locus_tag="VHM65_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX2704841.1"
                     /translation="MSTAPVVVLVILVVALVLLGASVRLVQQYERGIVLRFGRLLTEV
                     REPGLRFIVPIADQMTKVSIQTVVMGIPAQGAITRDNVTLVVDAVVYYRVIDPVKAII
                     NVQNYPLAVSQVAQTSLRAVIGRADLDTLLSDRDRINAELKAVIDAPTEEPWGLHIER
                     VEVKDVALPEGMKRSMSRQAEAERERRARVIAADGEFQASTKLAEAARVMGNTPGALQ
                     LRLLQTVVEVAAEKNSTLVMPFPVELLRFFDRAASEDTPTLASEGPDST"
     gene            complement(1218..>1614)
                     /locus_tag="VHM65_03725"
     CDS             complement(1218..>1614)
                     /locus_tag="VHM65_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2704842.1"
                     /translation="YDETGELPCLNQVELHPYLQQRDLVAFHREHGIVTESWSPLASG
                     GDVLRNAVVLDVAAKHGMTPAQVIIRWHLDHGFVVIPKSVTPSRIRENISVFGFALDS
                     DDMERLATLDRGLRTGPDPDRFRMGAVGR"
BASE COUNT          254 a          591 c          536 g          233 t
ORIGIN      
        1 ggctatcacc ggggcaagct cgtcgatcgc ctcgcccgac tcctgcgctg agcagggtta
       61 cgcccggcgc gcgtgcccaa gtcgggcaac gtccgcccat tcacagggga tgcccggaca
      121 ttccccgacc tcgggggagg gtgggccgcc gacgtagact ggcggtgacc gagggcacgt
      181 cgcgcgtcgg cgctcaagtc ggcgccgctc ccggcgaacc tcggtcgcct cgcggccgga
      241 gacgggagca tcgacatggc gtcgggctcg caccacaccc catgcccgag cttcgcccga
      301 gcggcgagca ctgcatcgac cctggcgacc acatgagcac ggcacccgtc gtcgtcctgg
      361 tgatcctcgt cgtcgcgctc gtcctgctcg gcgccagcgt ccgcctcgtc cagcagtacg
      421 agcgggggat cgtgctgcgc ttcggccgcc tcctcaccga ggtccgggag ccggggctgc
      481 gcttcatcgt gccgatcgcc gaccagatga cgaaggtctc gatacagacc gtcgtcatgg
      541 gcatccccgc acaaggggcc attacccgtg acaatgtcac cctcgtcgtc gacgccgtcg
      601 tgtactaccg ggtcatcgac ccggtcaagg cgatcatcaa cgtgcagaac tacccgctgg
      661 ccgtctccca ggtcgcccag acctcgttgc gggcggtcat cggccgcgcc gacctggaca
      721 ccctgctctc cgaccgtgac cggatcaacg ccgagctcaa agccgtcatc gacgcaccca
      781 cggaggaacc ttgggggctg catatcgaac gcgtcgaggt caaggacgtc gccctccccg
      841 aggggatgaa gcgctccatg tccaggcagg ccgaagccga gcgagagcgc cgggcacgcg
      901 tcatcgccgc ggacggcgag ttccaggcgt cgaccaagct tgccgaggcc gcccgggtca
      961 tgggcaacac gccgggagcc ctgcagctgc ggctcctcca gacggtcgtc gaggtggccg
     1021 cggagaagaa cagcacgctc gtcatgccct tcccggtgga gctcttgcgg ttcttcgacc
     1081 gcgccgcgag cgaggacaca cccacgctgg cctccgaggg gccagacagc acctgattct
     1141 cagcaccgcc gtgtccccgg gcagtcccgc cgggtcaccc ggcggcgccg ggtcacccgg
     1201 cggcgccaga cccctcgcta ccgtccgacc gcgcccatcc ggaaccggtc tgggtccggg
     1261 ccggtccgca gcccgcggtc gagcgtggcg agtcgctcca tgtcatcgga gtcgagcgcg
     1321 aagccgaaca ccgagatgtt ctcccggatc cgcgacggcg tcaccgactt cgggatcacc
     1381 acgaagccat ggtcgaggtg ccaccggatg atgacctgcg ccggcgtcat gccgtgcttg
     1441 gccgcgacgt cgaggacaac cgcgttgcgc aggacatccc cgcccgacgc gagcgggctc
     1501 cacgactcgg tgacgatgcc gtgctcgcgg tggaacgcga cgaggtcgcg ctgctgcaga
     1561 tacgggtgca gctcgacctg gttgaggcac gggagctcgc cggtctcgtc atac
//
LOCUS       DATAIX010000249         1833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_184780, whole genome shotgun sequence.
ACCESSION   DATAIX010000249 DATAIX010000000
VERSION     DATAIX010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184780"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..372
                     /locus_tag="VHM65_03730"
     CDS             157..372
                     /locus_tag="VHM65_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704843.1"
                     /translation="MADTTAISITAIVSSAIIGPAVMAWAARKQDRGRFTHEREMGDE
                     RFTHERVMRDTGDLRSLLPPVASEWAF"
     gene            complement(383..1579)
                     /locus_tag="VHM65_03735"
     CDS             complement(383..1579)
                     /locus_tag="VHM65_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704844.1"
                     /translation="MRLDGLYGKPGHRRQRYRCIPANGDRAHRFTERLPREEAWHDAC
                     EICERPVGLHEGPHAARHYQFVARGIAEALQTVGAGASYRQAALVARERARRVPVDPE
                     TGESRLTRHGQLVADWVEVFAPVVFEPRRPSAWPEAGSLLLDDLPFSVRDPATGRFRI
                     AFRVYCAAGFEAGRPKLWHLEAHTSKSQAAWERFLGSLEGAPPRVVCDNDIGLTGAVR
                     ARFPQAELYLCEWHLRHALERLMAKVHRNEPQHREAIDALLPRVEAAFTGPSFWAPFV
                     RDAHAAEIPRLSGWLDGNGRIVSEQFGRRGLRSLRPADMPLSTSPLDGLIEPIRAALH
                     PRRYGLKNRERTNRLLWLMQLHANRQDDPLTYAKDIRAWLEANGGRPRTTRRVVADRA
                     GLPSLR"
BASE COUNT          292 a          620 c          657 g          264 t
ORIGIN      
        1 ccgccgctgt tgcccaatcc gttgcccacc tacggaaaac gggccccttc tcagggaccg
       61 ttttccgtag gtgggcaacg gattgggcaa cagcggcggc ggcccggggg gcgctcgcaa
      121 ccgggccgac cgccggcagt ccagaatgtt ccaggcgtgg ccgacacgac cgccatcagc
      181 atcaccgcca tcgtttcctc cgcgatcatc ggacctgcgg tcatggcctg ggcggcgcgt
      241 aaacaggacc gaggccgctt cacccacgag cgggaaatgg gtgacgagcg cttcacccac
      301 gagcgggtca tgcgtgacac cggggacctc cgtagcctgc tcccccccgt cgcatccgag
      361 tgggcattct gaacgggcgg gttcagcgca gcgagggcag gccggcgcga tcggcaacaa
      421 cgcggcgggt ggtgcgtggc cggccgccgt tggcttcgag ccaggctcgg atgtccttgg
      481 cgtaggtgag cgggtcgtcc tggcggttgg cgtgcagctg catcagccaa agcaggcggt
      541 tggtgcgctc gcggttcttg agcccgtagc ggcgggggtg aagcgcggcg cggatcggct
      601 cgatgaggcc gtccagcggc gaggtcgaga gcggcatgtc ggcgggtcgc aggctgcgca
      661 gaccacggcg gccgaactgc tcggagacga tccgaccgtt gccgtcgagc cagcccgaga
      721 gccgggggat ctccgctgcg tgagcgtcgc ggacgaacgg cgcccagaac gacgggccgg
      781 tgaaggcggc ctcgactcgg ggcaaaagcg cgtcgatcgc ctcccgatgc tgcggctcgt
      841 ttcggtggac cttggccatc agccgctcca gcgcgtggcg caggtgccac tcgcagagat
      901 agagctcggc ctgcgggaag cgagcacgaa cagcgccggt gaggccgatg tcgttgtcgc
      961 aaaccacgcg tggcggcgcc ccctccaggc tgcccaggaa ccgctcccaa gcggcctgcg
     1021 acttgctcgt atgcgcctcc aggtgccaga gcttggggcg ccctgcttca aaccccgccg
     1081 cgcaatagac cctgaaggcg atccggaagc gcccagtggc cgggtcgcgc accgagaacg
     1141 gcagatcgtc gagcagcagg gaaccggcct caggccaggc cgacggccgg cgcggctcga
     1201 acacgaccgg cgcgaagacc tcgacccagt cggccaccaa ttggccgtgg cgcgtcaggc
     1261 gcgactcgcc ggtctcggga tcgacaggca cgcgtcgcgc ccgctcgcga gcaaccagcg
     1321 ccgcctggcg atagctcgcc ccggccccga ccgtctgcag cgcctcggcg atcccacgcg
     1381 ccacgaactg gtagtggcgc gcggcatgag ggccctcgtg caggcccacc ggacgctcgc
     1441 agatctcgca ggcgtcgtgc caggcctcct ctcgcggcag tcgctcggtg aagcgatgcg
     1501 ccctgtcgcc gttcgccgga atgcagcggt agcgctggcg gcggtggccg ggcttgccgt
     1561 agaggccgtc gagtcgcact cgagagccca cgtgctcagg ctgcgggcag gcagctcgcg
     1621 cgctgctcca cgtctttccc ttcgtcgcca ttccaggcga cgaagctacg agtacaacca
     1681 gacagaatgc tcacctagat gcgacggggg gggtcggtct atcagagcag gtggagatgc
     1741 atagagctgg agaatgcgag agtctcctca ggcatcgtgc cgatctcccg tcgcggtgct
     1801 cggtgctaag cggacgctgg agcagggtcg ttg
//
LOCUS       DATAIX010000250         1500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_103077, whole genome shotgun sequence.
ACCESSION   DATAIX010000250 DATAIX010000000
VERSION     DATAIX010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103077"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VHM65_03740"
     CDS             complement(<1..444)
                     /locus_tag="VHM65_03740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2704845.1"
                     /translation="MRVDIITKEYPPAIYGGAGVHVAELVRALRARGDVDVQVRCFGA
                     PRDEAGTTGYADLPQLASANAALKAVGVDLTIAGDCVGADLVHSHTWYANFAGHLAAL
                     LGGMPHVVTAHSLEPMRPWKAEQLGGGYRLSSFAEKTAYEAAAAVI"
     gene            506..>1500
                     /locus_tag="VHM65_03745"
     CDS             506..>1500
                     /locus_tag="VHM65_03745"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002023.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX2704846.1"
                     /translation="MRRAPSYKVLAIVLAGGEGKRLMPLTADRAKPAVPFAGIYRLID
                     FALSNVVNSGYLKVVVLTQYKSHSLDTHVTKTWRMSTMLGNYVAPVPAQQRVDKNWYL
                     GSADAIFQSLNLIHDEEPDIVVVVGADHVYRMDFSQMVAQHIETGAGVTVAAIRQPIS
                     LADQFGVIELDAQPSRKFGRFREKPTDAIGLADAPHEVLASMGNYVFNADILMDAVTR
                     DRDLEGSRHDMGGDIVPAFVAEGSAYVYDFKDNEMPGSTERDHGYWRDVGTLDSYYDA
                     HLDLVSVHPVFNLYNYEWPIYTAHSPFPPAKFVHGYQGRFGEALNSMVSPGVVISG"
BASE COUNT          245 a          553 c          467 g          235 t
ORIGIN      
        1 gatgaccgcc gccgccgcct cgtaggcggt cttctcggcg aaggacgaca ggcggtagcc
       61 cccaccgagc tgctcggcct tccaggggcg catcggctcg aggctgtgtg cggtgacgac
      121 atgcggcata ccgccgagca gtgcggccag gtgcccagcg aagttggcgt accacgtgtg
      181 cgagtggacc aggtcggcgc cgacacagtc accggcgatg gtcaggtcga ccccgacggc
      241 cttcagcgcg gcgttcgcgc tcgcgagctg cggcaggtcg gcataccccg tcgtccccgc
      301 ctcgtcccgt ggtgcgccga agcacctcac ctggacgtcg acgtcgccgc gggcccgcaa
      361 agcccggacg agctcggcga cgtggacacc cgctcccccg tagatcgcgg gggggtactc
      421 cttggtgatg atgtcgacgc gcaccccacg agactagtgc cgtgcatcgc tcgccgcgat
      481 ggcgcaacgc ccctagggtg gaagcatgcg acgcgccccc agctacaagg tcctcgcgat
      541 cgtgctggcc ggtggggagg gcaagcgcct catgccgctc acggcggatc gggccaagcc
      601 tgccgtgccc ttcgctggca tctaccggct catcgacttc gccctgtcca acgtcgtcaa
      661 ctcggggtac ctcaaagtcg tcgtgctgac ccagtacaag tcccacagcc tcgacaccca
      721 tgtcaccaag acctggcgga tgtcgaccat gctgggcaac tacgtcgccc ccgtcccggc
      781 ccagcagcgg gtcgacaaga actggtacct cggcagtgcc gacgcgatct tccagagcct
      841 caacctcatc catgacgagg agccggacat cgtcgtcgtc gtcggggccg accacgtcta
      901 ccgcatggac ttctcccaga tggtggccca gcacatcgag accggggccg gtgtcaccgt
      961 tgctgcgatc cgccagccca tctcgctggc ggaccagttc ggggtcatcg agctcgacgc
     1021 ccagccgtcc cgcaagttcg gccgcttccg ggagaagccg accgacgcca tcgggctcgc
     1081 cgatgccccg cacgaggtgc tcgcctcgat gggcaactac gtcttcaacg ccgacatcct
     1141 catggacgcg gtgacccgtg accgcgacct cgagggcagc aggcacgata tgggcggcga
     1201 catcgtgccc gccttcgtcg ccgagggctc ggcctatgtc tacgacttca aggacaacga
     1261 gatgcccggg tcgaccgagc gcgaccacgg ctactggcgc gacgtcggga ccctcgactc
     1321 ctactacgac gcgcacctgg acctcgtctc ggtccacccc gtgttcaacc tctacaacta
     1381 cgagtggccg atctacacgg cccactcccc cttcccgccg gccaagttcg tccacggcta
     1441 ccagggccgc ttcggcgagg cgctcaactc gatggtctcc ccgggcgtcg tcatcagcgg
//
LOCUS       DATAIX010000251         3758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_162921, whole genome shotgun sequence.
ACCESSION   DATAIX010000251 DATAIX010000000
VERSION     DATAIX010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162921"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..1419)
                     /locus_tag="VHM65_03750"
     CDS             complement(241..1419)
                     /locus_tag="VHM65_03750"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX2704847.1"
                     /translation="MRSAESVGIVGVVGAGTMGAGIAQVAAAAGHEVYLVDAFAGAAE
                     AALARIAAGLTGRVAKGALSDGEAAGISARLHAASAAEDLPECVLVVEAIREDLADKA
                     ALFAVLADHQPSDTILASNTSSLDIGAIAASVTHPERVLGLHFFNPPPAMKLVEVVHA
                     AGTGPAYLAHAGILMQAWGKVPVRCASTPGFIVNRVARPFYGEAQRMAEAGVADPATI
                     DACLRAGGFPMGPFELTDLIGQDVNLAVGTSVWEQTGHDPRYAPTSFQEHLVASGHLG
                     RKTGQGVYRYSAEGRALGADPDVDLVQELLNGPLATNPLTRTLAMLVNEAVDLVDRGE
                     SSTGDVDTAMRLGTRYPRGPIEWGREIGFDVVAQQITELDVLFPGGRYRPSPGLHTPA
                     "
     gene            complement(1468..2445)
                     /locus_tag="VHM65_03755"
     CDS             complement(1468..2445)
                     /locus_tag="VHM65_03755"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX2704848.1"
                     /translation="MILVFVECTTDSVNDVSAQALTFARDLLVREGSPGVHAVVVGDL
                     PAGALAACADQGVSVVHRASHASLDRYAARAWAACVEQVARQIGATIVLAAGTPRGNE
                     VMAHVATRLDVAMAANAVAVASLSPVSVTRQMMGGSVLEDMALAGSPAVFTIAGHTKA
                     AEPADVPAQTEVSELEPVITGAELRGQATRLDARATGDTAHLSTARVVVGAGRGAGGP
                     DGFGPCIELASLLGGSLGVSRVVTTLGWRPHSEQVGQTGTRISPELYIPCGISGAIQH
                     WAGCASSQQIMAVNTDPDAPMVTRANYAVIGDMHEVVPAIVDALRAQHH"
     gene            complement(2442..3212)
                     /locus_tag="VHM65_03760"
     CDS             complement(2442..3212)
                     /locus_tag="VHM65_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704849.1"
                     /translation="MVNVLVCVKRVPEATNEVVLTPDGMTVDGRYVGYTTSPHEECAV
                     ELATQIAATGGTSTVLTLGPAESVEQLRAALAVGVDSGVLIEADADSFGPADVARAIA
                     EVVRSRGGDAAAAYDLVLVGNDAGDTGDFQVGVRLGYALDWPVVAGISTIEIGEGTAS
                     LVSDTLEGTLVYDVALPAVVTVLEGGVAPRYPSLVGRMKAKRAPVEIVTPSFEPVGSG
                     RISLTVPPPPPNEVRILGEGPGAAAAVVATLHYLGVTR"
     gene            complement(3216..>3758)
                     /locus_tag="VHM65_03765"
     CDS             complement(3216..>3758)
                     /locus_tag="VHM65_03765"
                     /inference="COORDINATES: protein motif:HMM:NF020253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX2704850.1"
                     /translation="LWDCIGEAGQPHGLVPAGIGVYGTTGRIEKGYRAYGYELDSERT
                     IIEAGMARATVKEADFIGRDAYLAQREQAPAAVLCTLTVDDHTSASGRKRYMLGGEPI
                     LTRDGGALTDGHGHHPYVTTAGSAPSLGKHLLMAYLPPEQAVVGAELAVSYMEELYPV
                     TVGSADATPLFDPDNERIRG"
BASE COUNT          520 a         1317 c         1373 g          548 t
ORIGIN      
        1 gcccgtccct ccgcggagta ccggtacacc ccctggccgg tcttgcgccc gaggtggccg
       61 ctcgcgacga gatgttctga agccaacggg gagaggtccc agatcgacgc gcgaccggcg
      121 ccactgccag tgtctgcgca gtatgccgtg cccgctggtg gcgggcacgg catagcgagc
      181 ggaaacaggc ggtagcgggc gccccgcccg cgtcgtgtgc gtgccccgcc acgcgcgggg
      241 tcaggccggt gtgtgcagtc cggggctggg gcggtagcga ccgccgggga agagcacatc
      301 gagctcggtg atctgctggg cgacgacgtc gaagccgatc tcgcggcccc actcgatcgg
      361 gccgcgcggg taacgggtgc ccaggcgcat cgcggtatcc acatcccccg tgctcgattc
      421 ccccctgtcc acaaggtcca cggcctcgtt gacgagcatg gccagggtgc gcgtcaatgg
      481 gttggtcgcg agcggcccgt tgagcagctc ctggacgagg tccacgtccg ggtcggctcc
      541 cagcgcccgt ccctccgcgg agtaccggta caccccctgg ccggtcttgc gcccgaggtg
      601 gccgctcgcg acgagatgtt cctggaagga ggttggcgcg tatcgagggt cgtgtccggt
      661 ctgctcccac acggatgtgc cgacggccag gttgacgtcc tgcccgatga ggtcggtgag
      721 ctcgaacggc cccatcggga agccgccagc acgcaggcac gcgtcgatgg tggccgggtc
      781 ggcgacaccg gcctcggcca tgcgctgagc ctcgccatag aacgggcggg ccacccggtt
      841 gacgatgaat ccgggcgtcg aggcgcaacg taccgggacc ttcccccagg cctgcatgag
      901 gatgcccgcg tgagccaggt aggccgggcc ggtgcccgcc gcgtggacga cctcgacgag
      961 cttcattgcg ggtggtgggt tgaagaagtg cagcccgagc actcgctccg gatgcgtcac
     1021 cgacgccgcg atcgcaccga tgtcgaggct cgaggtgttg ctcgcgagga tcgtgtcact
     1081 gggctggtga tcggccagca cggcgaagag ggccgccttg tcggcgaggt cctcgcggat
     1141 cgcctcgacc acgaggacgc actccggcag gtcctcggcg gcgctcgccg catggaggcg
     1201 agcgctgatg cctgccgcct cgccgtcgct cagggcaccc ttggcgaccc ggcccgtcag
     1261 gccggcggcg attcgtgcga gggcggcctc ggccgcgccg gcgaaggcgt cgacgagata
     1321 gacctcgtga ccggcagccg cggcgacctg cgcgatgcct gcgcccatcg tccctgcccc
     1381 gacgacaccg acgatgccga ctgactccgc gctcctcatg ggctcatcct ggcaggacga
     1441 gccttgggcg gcagcgtgag cgccggctca gtggtgctgc gcgcgcaggg cgtcgacgat
     1501 cgccgggacc acctcgtgca tgtcaccgat cacggcgtag ttggctctgg tcaccatggg
     1561 cgcgtccggg tcggtgttga cggccatgat ctgttgcgag gaggcgcagc cggcccagtg
     1621 ctggatggcg ccgctgatcc cgcaggggat gtagagctcg ggcgagatcc gggtgccggt
     1681 ctggcccacc tgttccgagt gcggccgcca gcccagggtc gtgacgaccc gcgagacgcc
     1741 gagcgagccg ccgagcagac tggcgagctc gatgcagggc ccgaacccgt cgggaccgcc
     1801 cgccccgcgc ccagcgccga cgacgacgcg ggccgtcgac aggtgcgccg tgtcaccggt
     1861 cgcccgcgcg tcgagccggg ttgcctgacc gcgcagctcg gcgccggtga tgaccggctc
     1921 gagctcgctg acctcggtct gcgccgggac gtcggcgggt tcggccgcct tagtgtgacc
     1981 ggcgatggtg aacaccgcgg gggacccagc gagggccatg tcctcgagaa ccgagccgcc
     2041 catcatctga cgggtcaccg acaccggcga gaggctcgcc acggcgaccg cgttggccgc
     2101 catcgccacg tcgagccggg tcgcgacgtg cgccatgacc tcgttgcccc ggggtgtccc
     2161 ggcggcgagc acgatcgtgg ccccgatctg gcgggcgacc tgctcgacgc aggccgccca
     2221 cgcccgagcg gcgtagcggt cgagcgaggc atggctggct ctgtgcacga ccgacacacc
     2281 ctggtccgcg caggcggcga gcgccccggc tggcaggtca ccgacgacca cggcgtgcac
     2341 gccgggcgac ccctcccgga cgagcaggtc ccgggcgaag gtcagcgcct gggccgagac
     2401 gtcgttgacc gagtcggtcg tgcattcgac gaagacgagg atcatcgggt cacccccagg
     2461 tagtgcagcg ttgcgacgac ggccgcggcc gcgcccggtc cttcaccgag gatcctcacc
     2521 tcgttgggcg gtgggggcgg gaccgtgagc gagatccgcc ccgaccccac cggctcgaag
     2581 gagggcgtca cgatctcgac cggtgccctc ttggccttca tccgcccgac cagtgagggg
     2641 tagcgcggcg cgaccccacc ctcgaggacg gtgaccacgg ccgggagcgc gacgtcatac
     2701 acgagggtgc cttcgagggt gtcgctgacg agcgaggccg ttccctcgcc gatctcgatc
     2761 gtcgagatgc cggccacgac cggccagtcg agcgcgtagc cgaggcgcac cccgacctgg
     2821 aagtcacccg tgtcccccgc gtcgttgccg accaggacga ggtcgtatgc cgccgctgcg
     2881 tcacctccgc gggaacgcac cacctcggcg atggcccgag cgacgtcggc cgggccgaac
     2941 gagtcggcgt cggcctcgat gaggacgccg gagtcgacgc cgaccgcgag ggcggcgcgc
     3001 agctgctcga ccgactcggc cgggccgagg gtgagcacgg tgctcgtccc acctgtcgcc
     3061 gcgatctggg tcgcgagctc gacggcgcac tcctcgtggg ggctcgtcgt gtatccgacg
     3121 tagcggccgt cgacggtcat cccgtcggga gtgaggacga cctcgttggt cgcctcgggg
     3181 acccgcttga cacataccag cacgttgacc atggactatc cccggattcg ctcgttgtct
     3241 gggtcgaaga gaggggtcgc gtcggcggag ccgaccgtga cggggtagag ctcctccatg
     3301 tagctcaccg cgagctcggc tcccacgacg gcctgctcgg gcgggaggta ggccatgagg
     3361 aggtgtttgc cgagcgacgg ggccgatcct gccgtcgtga cgtacgggtg gtgcccgtgg
     3421 ccgtcggtga gggcgccgcc gtcgcgggtc aggatcggct cgccgccgag catgtagcgt
     3481 ttgcggcccg acgccgaggt gtggtcgtcg accgtgagcg tgcacaggac ggccgccggc
     3541 gcctgctccc gctgcgcgag gtaggcgtcg cggccgatga agtcggcctc cttgaccgtt
     3601 gctcgcgcca tgcctgcctc gatgatcgtg cgttcgctgt ccagctcgta gccgtaggcc
     3661 cggtagccct tctcgatgcg tccggtcgtg ccgtagaccc cgatgcccgc gggcaccagg
     3721 ccatgtggct gaccggcctc cccaatacag tcccacag
//
LOCUS       DATAIX010000252         2128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_274204, whole genome shotgun sequence.
ACCESSION   DATAIX010000252 DATAIX010000000
VERSION     DATAIX010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274204"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VHM65_03770"
     CDS             complement(<1..525)
                     /locus_tag="VHM65_03770"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2704851.1"
                     /translation="MASQRPVGAITRGTTGPNRLRRADRWLTGPAAAVLRRSRTPPVV
                     VDLGFGASAVTTLELFERLRTVRPDVEVIGIEIDPARVRAAAPLARPGLSFALGGFEL
                     ALERGARPVVVRAFNVLRQYDEEAVPDVWRQVQDRLAPGGLFVDGTSDEAGRLATWVG
                     MDVSGPQTLTLSMRL"
     gene            658..1965
                     /gene="mshA"
                     /locus_tag="VHM65_03775"
     CDS             658..1965
                     /gene="mshA"
                     /locus_tag="VHM65_03775"
                     /EC_number="2.4.1.250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol-3-phosphate glycosyltransferase"
                     /protein_id="HEX2704852.1"
                     /translation="MPTPDEIRRIAMISVHTSPLDQPGTGDAGGMNVYISEVAKRLAE
                     RGREVEIFTRATTGALPPVVELAPGVTVRHVTAGPYEGLGKNDLPGQLCAFAAGVMRA
                     HAAAPEGWYHLVHSHYWLSGQVGWLAADRWRVPLVHTMHTMARVKNLHLAAGDTPEPA
                     GREIGEAQVVEAAGMLVASTVDEARDLVELYSADPRRVSVVTPGVDLATFRPRDRRGA
                     RATLGLPLDAKLLIFAGRIQPLKAPDVLIRAAAELVRRHPDWRDSLVVGLLGGASGTG
                     LARPHELGDLAASLQISDLVRFVPPVDRARLALWYAAADLVVVPSYSESFGLVAVEAG
                     ASGTPVVAAYVGGLPTAVGDGGVLVRGHATGPWADALEALLLDPGRRAELSRRALRHT
                     ARFGWDATADALLGAYGAALHGRTHVPIGGGARLAQVPAAMIP"
BASE COUNT          272 a          802 c          778 g          276 t
ORIGIN      
        1 caggcgcatc gacagggtga gggtctgggg gccgctgacg tccatgccaa cccaggtcgc
       61 caggcgcccg gcctcgtcgc tcgtgccgtc gacgaacagc ccgccgggag cgagccggtc
      121 ctggacctgg cgccatacgt ccgggaccgc ctcctcgtca tactggcgca ggacgttgaa
      181 cgcgcgcacg accacgggac gggcgccgcg ctccagcgcg agctcgaagc cgccgagggc
      241 aaagctcagc cccggacgcg ccagcggcgc ggcggcgcgc acccgggccg ggtcgatctc
      301 gatcccgatg acctcgacat cggggcgcac cgtgcgcagc cgttcgaaca gctcgagcgt
      361 cgtcaccgca gatgcgccga agccgaggtc gacgacgacg ggcggcgtgc ggctgcggcg
      421 cagcaccgcg gcggcggggc ccgtgagcca ccggtcggcg cggcgaaggc ggttggggcc
      481 ggtcgttccc cgggtgatcg ccccgaccgg acgctggctc gccaccgcgt cagtatggcg
      541 cgggaccgct gcggccggaa tcaacctggc cgcccgctgg ttgtcgcagg gacgcgtggc
      601 gcgtcctgct cggtcaacgc cggccggagc gggcccgtcc gagtgtgagg agaatgggtg
      661 ccgacccccg acgagatccg acggatcgcc atgatcagcg tgcacacctc gccgctggac
      721 cagccgggca cgggcgacgc gggtggcatg aacgtctaca tcagcgaggt ggccaaacgc
      781 ctcgccgagc gcggccgcga ggtcgagatc ttcacgaggg caacgaccgg agcgctccct
      841 ccggtggtcg agctggcccc gggggtcacg gtccgccacg tcaccgccgg cccctacgag
      901 ggcctgggca agaacgacct gcccggccag ctctgtgcct tcgcggcggg tgtcatgcgg
      961 gcccacgcgg cggcacccga ggggtggtac cacctcgtcc actcgcacta ctggctctcc
     1021 ggccaggtcg gctggctcgc cgccgaccgg tggcgggtcc cgctcgtcca tacgatgcac
     1081 accatggccc gggtcaagaa cctgcacctt gccgcaggcg acacccccga gccggccggt
     1141 cgtgagatcg gggaggccca ggtcgtcgag gccgccggca tgctcgtggc cagcaccgtc
     1201 gacgaggcgc gcgacctcgt cgagctgtac agcgccgacc cgcgcagggt ctcggtcgtg
     1261 acgcccgggg tcgacctggc gaccttccgg ccccgcgacc ggcgaggggc gagggcgacc
     1321 ctcggtctgc ccctggatgc caagctgctc atcttcgccg gccggatcca gccgctcaag
     1381 gcgccggacg tcctcatccg ggccgcggcc gagctcgtcc ggcgccatcc cgactggcgc
     1441 gactcactcg tcgtcgggct gctcgggggc gcgagcggca ccggcctggc tcgtccccac
     1501 gagctcggcg acctcgcggc ctcgctgcag atctccgacc tggtccggtt cgtcccgccc
     1561 gtcgaccgag cgcgcctcgc cctctggtat gccgcggcgg acctcgtcgt cgtgccctcc
     1621 tacagcgagt ccttcggcct ggtcgccgtc gaggccggag ccagcggtac cccggtcgtg
     1681 gccgcctatg tcggcgggct gccgaccgcg gtcggggatg gtggggtcct cgtccgtggc
     1741 cacgccaccg gtccgtgggc cgacgcgctg gaggcactgc tcctcgaccc cggccggcgg
     1801 gccgagctct cgaggcgcgc cctgcgtcat accgcccggt tcggctggga cgcgacggcc
     1861 gacgcgctgc tgggggccta cggcgctgcc ttgcacgggc gcacccacgt cccgatcggc
     1921 ggcggggccc ggctcgcgca ggtcccggcg gcgatgatcc catgacgagc actcccgacc
     1981 cggtcgcgac ggcgaccgcg gccatcacgg catacctgca ggaggcgggc ctcgagtggg
     2041 acctcggcgc ccggtccggt gaggtcatcg catcactgcc gggcgagcgc aagctcaaga
     2101 ccgcggtctc gctgctcgtg cgagaggt
//
LOCUS       DATAIX010000253         1636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_162970, whole genome shotgun sequence.
ACCESSION   DATAIX010000253 DATAIX010000000
VERSION     DATAIX010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162970"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..>1636)
                     /locus_tag="VHM65_03780"
     CDS             complement(66..>1636)
                     /locus_tag="VHM65_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2704853.1"
                     /translation="RELLDQRFAGMKQALEGATDEDRARVNEMLTDLNELLEAHRRGD
                     DTDQQFADFMAKHGDFFPERPQSIEELLDSLAARAAAAQRLRSSMTQEQRDELDALAQ
                     QAFGSPGLMQSLARLDANLQGLRPGEDWDGAERFDGQQGVGLGDGTGILQDLADLDEL
                     GEQLSQQYAGARMGDVDLDKLARQLGDEAAVDARTLAELEKALRDSGYLKRGSDGNLR
                     LSPKAMRQLGKALLRDVATRLSARQGQRDLRQAGAAGEPSGATREWEFGATEPWEVTR
                     TMTNAIRRTVADGRDPRGGIRLDIRDVEVTETEARTQAAVALLVDTSFSMAMDGRWVP
                     MKRTALALHQLVSTRFRGDHLQLVGFGRHARVMDVEELTGLDALWDKGTNLHHALLLA
                     NRHFRRHPHAQPVLLIVTDGEPTSHLEPDGEAFFSYPPHPRTVAQTVAELDAAGRLGA
                     QTTFFRLGQDPGLARFVDQMARRVEGRVVAPELDDLGAAVVGSYLGSRSGAHSPAGSA
                     YGDWFGGRGFWVGS"
BASE COUNT          209 a          600 c          585 g          242 t
ORIGIN      
        1 tatttgcgcc gcgaggagcc actatccggc gcgaggacgg ggcggctagg ccccgctcgg
       61 gcgccctagc tgccgaccca gaacccgcgc ccgccgaacc agtccccata ggccgacccg
      121 gccggcgagt gggccccgct acgggaaccc aggtaggagc ccaccacggc cgcgcccagg
      181 tcatccagct ccggcgcgac gacccggccc tcgacccgcc gggccatctg gtcgacgaac
      241 cgggccaggc ccgggtcctg gccgaggcgg aagaaggtcg tctgcgcgcc cagccgaccc
      301 gcggcgtcga gctcggccac ggtctgggcg acggtgcggg ggtgcggcgg gtaggagaag
      361 aacgcctcgc cgtccggctc gaggtgggag gtcggctcgc cgtcggtgac gatgagcagg
      421 accggctggg catgggggtg ccggcggaag tgccggttgg ccagcagcag ggcgtggtgg
      481 aggttggttc ccttgtccca caacgcatcc agtccggtca gctcctcgac gtccatgacc
      541 cgggcgtggc gcccgaagcc gaccagctgg aggtggtcac cccggaaccg ggtcgagacg
      601 agctggtgca gcgccagggc cgtgcgcttc atcggcaccc agcgaccgtc catggccatc
      661 gagaacgacg tgtcgacgag gagggccacg gccgcctggg tccgcgcctc ggtctcggtc
      721 acctcgacgt cgcggatgtc caggcgtatg ccgccgcggg ggtcgcgccc gtcggccacg
      781 gtccgccgta tggcgttcgt catcgtgcgg gtcacctccc acggctcggt cgcaccgaac
      841 tcccactcgc gggtagcacc gctgggctcg ccggctgccc cggcctggcg caggtcccgc
      901 tgtccctggc gcgccgacag ccgggtggcg acgtcacgca acagtgcctt gccgagctgg
      961 cgcatggcct tgggcgacag gcgcaggttg ccgtccgacc cgcgtttgag gtagccgctg
     1021 tcgcgcagcg ccttctccag ctcggccagg gtgcgcgcgt cgacggctgc ctcgtccccg
     1081 agctggcggg ccagcttgtc caggtcgacg tcccccatcc gggccccggc atactgctgt
     1141 gacagttgtt cgccgagctc gtcgaggtcg gccaggtcct gcaggatgcc agtaccgtcg
     1201 ccgagcccaa ctccctgttg cccgtcgaaa cgctcggcgc cgtcccagtc ctcgcccggt
     1261 cgcagcccct gcaggttcgc gtccagccgg gcgagggact gcatgaggcc gggcgacccg
     1321 aaggcctgct gtgccagcgc gtcgagctcg tcgcgctgct cctgcgtcat cgagctgcgc
     1381 agccgctggg cggccgccgc ccgggccgcg agtgagtcga gcagctcctc gatgctctgc
     1441 ggcctctccg ggaagaagtc accgtgcttg gccatgaagt cggcgaactg ctggtccgtg
     1501 tcgtcgccgc gccgatgggc ctccaacagc tcgttgaggt cggtgagcat ctcgttgacc
     1561 cgggcacggt cctcgtcggt ggcgccctcc agcgcctgct tcattcccgc gaaccgctgg
     1621 tcgagcagct cgcgcc
//
LOCUS       DATAIX010000254         2012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_13511, whole genome shotgun sequence.
ACCESSION   DATAIX010000254 DATAIX010000000
VERSION     DATAIX010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13511"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /gene="hemG"
                     /locus_tag="VHM65_03785"
     CDS             <1..768
                     /gene="hemG"
                     /locus_tag="VHM65_03785"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEX2704854.1"
                     /translation="LGLLAEHLAARLEAAGVEIRCGAHVEGLRPRPGGWSVSAISGGA
                     TEHTRCQAIVLAVPAAPSARLLAPLAPAAAEVLGALDYASVALVTLALPREEMADLTG
                     SGFLVPPGEEITIKASTFSAVKWSWVGAAAPDLAVVRASFGRAGESEVLRGDDAALVR
                     LARRDLGHVLGAALPAHIDARVHRWESSLPQYAVGHLDRVAAVRDAIRGLPGLALAGA
                     AYDGVGIPACIGSGRAAAQAVLDHLSATQTHRGESAV"
     gene            765..1466
                     /gene="hemQ"
                     /locus_tag="VHM65_03790"
     CDS             765..1466
                     /gene="hemQ"
                     /locus_tag="VHM65_03790"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: protein motif:HMM:NF042928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HEX2704855.1"
                     /translation="MTTNPNAQAIREINDTIRYAMWSVFAVAEPVRADREGLAREVEE
                     LFTELAAQDVVVRGVYDVAGLRADADFMVWWHAPEIEALQEAYHRLLKTGLGASLEPV
                     WSVAALHRPAEFNKSHVPAFLAGEDPKRYVCVYPFVRSYDWYVIDDAERRDMLREHGQ
                     MARDYPDVRANTIAAFALGDYEWVLAFEADELHRIVDLMRELRASRARLHVREEVPFY
                     TGPRVPVSELVAALR"
     gene            complement(1646..>2012)
                     /gene="msrB"
                     /locus_tag="VHM65_03795"
     CDS             complement(1646..>2012)
                     /gene="msrB"
                     /locus_tag="VHM65_03795"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX2704856.1"
                     /translation="LTPAEYDVLREAGTERPFVGEYTDTKTVGVYRCRACSTELFRSE
                     TKFESHCGWPSFYAPLAEDRVAYLEDRSGLGPPRVEVRCATCGSHLGHVFEGEGYGTP
                     TDQRYCINSISLTLEGQSG"
BASE COUNT          260 a          703 c          736 g          313 t
ORIGIN      
        1 ctcggcctgc tcgccgagca cctcgccgca cgcctggagg cagccggggt cgagatccgc
       61 tgcggcgctc acgtcgaggg gctgcggccg cgtccgggag ggtggtcggt ctccgccatc
      121 tcgggaggag cgaccgagca cacccggtgt caggccatcg tccttgcggt acctgctgcg
      181 ccgtcggcgc ggctgctcgc gcccctggcc cccgccgctg ccgaggtgct cggtgcgctc
      241 gactacgcct cggttgccct ggtgacgctg gccctgccgc gcgaggagat ggcggacctc
      301 acggggtccg ggttcctcgt gccgccgggg gaggagatca ccatcaaggc gtccacgttc
      361 agcgcggtga agtggtcgtg ggtcggggcg gccgcaccag acctcgccgt cgtgcgcgcc
      421 tccttcggcc gcgcgggtga gagcgaggtc ctccgcggcg acgacgcggc cctcgtccgg
      481 ctcgcccggc gcgacctcgg tcacgtcctc ggtgccgcgc tcccggcgca catcgacgcc
      541 cgcgtgcacc gctgggagtc cagcctgccc cagtacgccg tcgggcacct ggaccgggtc
      601 gcggcggtgc gggacgccat acggggactg cccggtcttg ctcttgccgg ggctgcctac
      661 gacggcgtcg gcatccctgc ctgcatcggg tccggccgcg ccgcggcaca ggccgtcctg
      721 gaccacttgt cggcgaccca gacccaccga ggagaatcgg ccgtatgacg accaacccca
      781 acgcccaggc catccgggag atcaacgaca cgatccgcta tgcgatgtgg tcggtcttcg
      841 ccgtggccga gccggtccgc gctgaccgcg agggcctggc gcgcgaggtc gaggagctct
      901 tcaccgagct ggccgcccag gacgtcgtcg tgcgcggcgt gtatgacgtc gcggggctgc
      961 gcgcggacgc ggacttcatg gtgtggtggc acgcgccgga gatcgaggcg ctgcaggagg
     1021 cctaccaccg gttgctcaag acggggctcg gcgcctcgct cgagccggtg tggtcggtcg
     1081 cggcactgca ccgcccggcc gagttcaaca agagccacgt gccggcgttc ctcgcgggcg
     1141 aggaccccaa gaggtacgtc tgcgtctatc cgttcgtgcg cagctacgac tggtatgtca
     1201 tcgacgacgc cgagcgccgc gacatgttgc gcgagcatgg gcagatggcg cgggactacc
     1261 ccgacgtgcg ggccaacacg atcgcggcgt tcgcgctcgg ggactacgag tgggtgctgg
     1321 cgttcgaggc cgacgagctg caccggatcg tcgacctcat gcgtgagctg cgggcctccc
     1381 gggctcgcct gcacgtccgc gaggaggtcc cgttctacac cgggccgcgg gtgccggtgt
     1441 ccgagctggt tgccgcgctg cgctgagtcc cggctgtcgt ggtgcggggc gcagcggcca
     1501 cctgaatgag gctccactca cgagggaacg acgccgttcc gtgggcatcg ttgatgagtc
     1561 aacgtgaggt tgcggagtcg gcccgtgatt gcctttcgac ctgagcaggg gccgcacggt
     1621 tgaacagcgc cggtcgtcga cccggctacc cggactggcc ctccagtgtc aggctgatcg
     1681 agttgatgca gtagcgctgg tcggtcggtg tgccgtagcc ctccccctcg aagacgtggc
     1741 cgagatgcga cccgcacgtg gcgcaccgca cctcgacgcg tggcggccct aggccggagc
     1801 ggtcctcgag gtaggcgacc cggtcctcgg cgagcggcgc gtagaacgag ggccatccgc
     1861 agtgcgactc gaacttcgtc tcgctgcgga acagttcggt gctgcaggcc cggcaccgat
     1921 acacgccaac ggtcttggtg tcggtgtact cgccgacgaa gggccgctcg gtgccggcct
     1981 cgcgcagcac gtcgtactcc gccggggtca gc
//
LOCUS       DATAIX010000255         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_162976, whole genome shotgun sequence.
ACCESSION   DATAIX010000255 DATAIX010000000
VERSION     DATAIX010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162976"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1499)
                     /gene="nhaA"
                     /locus_tag="VHM65_03800"
     CDS             complement(201..1499)
                     /gene="nhaA"
                     /locus_tag="VHM65_03800"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX2704857.1"
                     /translation="MPPRLFSPSTWRETRYVTEALRTETAGGALLLGAAVLALGWANS
                     PAADGYLALRNHQVGPAALHLDLSLGQWAADGLLAVFFLVAGIELKREFVAGDLADPA
                     KAVVPVAAAVCGVALPAIGYVVASGLAGGGAGTLAGWAIPTATDIAFALAVLALVGSH
                     LPAALRAFLLTLAVVDDLIAITIIAVFYTNGLHLGLLALAALPLAVFALMLRRGLTHP
                     ALLAIPALLAWGLVHSSGVHATVAGVLLGLAVPVARHQGDVPGEDDPAPSLAERIEHK
                     LRPLSAGVAVPVFAFFAAGVPVRGGGFLESLRDPAAVGVVTGLVLGKALGVFGGAWAF
                     ARFTKAELDEDLAWSDIAGVSLLAGAGFTVSLLIGALAFGEGSARDDHVKVAILIGSL
                     LAAVLASVVLRRRNQHYQAILMKARRDEGGDDISDMFVRR"
BASE COUNT          272 a          569 c          670 g          196 t
ORIGIN      
        1 cgatcccctt gatgccggtg gagacctcgg ccttggccat ctcgatctcg ccgcgcacga
       61 gtgcgccgat gtcggtcgaa gccgacgcga cgagctgacc gagtgtgcgc tcctggccgc
      121 ccggcgagtc ggggctgccg ctcgtgctca tgtccaacct ccgatcgtcg gggccggggt
      181 cccggcctcg ccctaagaga ctagcgccgg acgaacatat ccgagatgtc gtccccgccc
      241 tcgtcgcgtc gcgccttcat gaggatcgcc tggtagtgct ggttacgccg gcgcaggacc
      301 accgaggcga gcaccgcggc gagcagggag ccgatcagga tggccacctt gacgtggtcg
      361 tcccgcgccg agccctcacc gaaggccagg gcaccgatga gcagtgacac ggtgaacccg
      421 gcaccggcga gcagggacac cccggcgatg tcgctccagg cgaggtcctc gtcgagctcg
      481 gccttggtga agcgggcaaa cgcccaggct cccccgaaga cgccgagggc cttgccgagg
      541 acgaggccgg tgacgacgcc gactgcggca gggtcgcgca ggctctcgag gaaaccgccg
      601 ccgcgcaccg gaacgccggc cgcgaagaac gcgaagacgg gcacggcgac gccggccgag
      661 agaggtctga gcttgtgctc gatgcgctcg gccaggctcg gagccgggtc gtcctcgccc
      721 ggcacgtcgc cttggtggcg ggcgaccggc acggccaggc cgagcagcac cccggcgacc
      781 gtggcgtgga ccccggagga atggacgagc ccccaggcaa gcaatgccgg tatggcgagc
      841 agcgccgggt gggtgagacc ccggcggagc atgagcgcga agaccgcgag gggaagggcg
      901 gcgagggcga gcaggccgag gtggagaccg ttcgtgtaga agaccgcgat gatcgtgatg
      961 gcgatgaggt cgtcgacgac ggcgagggtg agcaggaacg cccgaagcgc cgccgggagg
     1021 tggctcccga cgagggccag cacggccagg gcgaaggcga tgtcggtggc cgtcgggatg
     1081 gcccagccgg ccagggtgcc ggccccgcca cccgcgaggc cggaggcgac gacatacccg
     1141 atggccggga gcgcgacccc gcacactgct gccgcgacgg gcacgacggc cttggcgggg
     1201 tcggcgaggt caccggcgac gaactcgcgc ttgagctcga tcccggcgac gaggaagaag
     1261 accgcaagca ggccgtcggc ggcccattgg ccgagggaga ggtccagatg cagggcggcg
     1321 ggcccgacct ggtggttgcg cagcgcgagg tagccgtcgg cggcggggga gttcgcccag
     1381 ccgagggcga ggaccgcggc gccgaggagc agggcgccgc cggccgtctc ggtgcgcaac
     1441 gcctcggtga cgtagcgggt ctcgcgccag gtcgaggggc tgaacaggcg cggtggcacg
     1501 aaggagggtg tggtcacgcg agcctttcgg gtgcggtcgg tccgagggct gctccgtgtt
     1561 ggtcgcactt gatgtgcgga cacagcggct cgcccaccga ccagacttcc cggcacacca
     1621 aggccgccag tctaccgggc tggcgggctg gcgggctgcc gggctcgagc acgggccgct
     1681 cgagctcccc gtcgagccga cccgagg
//
LOCUS       DATAIX010000256         2314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_13552, whole genome shotgun sequence.
ACCESSION   DATAIX010000256 DATAIX010000000
VERSION     DATAIX010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13552"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VHM65_03805"
     CDS             complement(<1..559)
                     /locus_tag="VHM65_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX2704858.1"
                     /translation="MNAELAEVRDFVAGIPPFDTLPARLLDELPAMLEVRYFRRGTRI
                     LSVGQDNDTMFILRAGAVDLHHENGDFLDRRGTGAAFGMSTLIRTGPFMAQVTAIEDT
                     LVLAMPRAVFDGLAREQPQFVAYYRLRLAERMRAAVAAVHGSGRGEPILKTRASDLLR
                     RGPVSATLDATIHEASRMMRDKRTSS"
     gene            690..1121
                     /locus_tag="VHM65_03810"
     CDS             690..1121
                     /locus_tag="VHM65_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704859.1"
                     /translation="MGLHETLLAPDRRAATVDGFVAFVEAEVAGKSGLGGAAVKTAYA
                     AARKISPTIVRRAVAGMLPDFLDGLDPFWAARGEGAFSEALGSRPAEVTDALLSVTDR
                     RAANPRHGALTKIYAAVRPRAARHVTDALPGLATTVERLMG"
     gene            complement(1139..>2314)
                     /locus_tag="VHM65_03815"
     CDS             complement(1139..>2314)
                     /locus_tag="VHM65_03815"
                     /inference="COORDINATES: protein motif:HMM:NF023454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HEX2704860.1"
                     /translation="SLLAAGVVERLPEPDETGRTVALTMDLQRGFALNQPLSAFALAA
                     IELLDPASPSYALDVVSVIESTLEDPRGVLGAQQWLARGQAVAEMKADGLDYDERMEL
                     LDDVSWPKPLEELLTAALELYRHTHPWISERDLSPKSVARDLHERAMTFGEFIAFYKL
                     TRGEGMVLRYLSDAYKALRQTVPLSARTEELTDIVEWLGEVVRQTDSSLLEEWEQLTS
                     GYDGQLTPGGLDPLATARAGAERPLSANERALRVMVRTAMWRKVSLFALRRWNDLAAL
                     DEAAVARGREALDLSPIDVAPWDASPLDALGWQEAVEGYFAEYADVGTGPDARGPGLT
                     VIHPEPGRWRVEQIIDDPAGDRDWRIRAVVDLAATDAAGELILLTTGAGPVSVQSQD"
BASE COUNT          337 a          811 c          849 g          317 t
ORIGIN      
        1 gggaggaggt tcgcttgtcg cgcatcatcc gggaggcctc gtggatcgtc gcgtccaggg
       61 tggcactcac ggggccccgc cggaggagat cgctggcccg ggtcttgagg atcggctcgc
      121 cacgcccgga cccgtggacc gccgcgacgg ctgcgcgcat ccgttcggcg agccgcagcc
      181 ggtagtaggc cacgaactgc ggctgctccc gcgccagccc gtcgaacacc gccctcggca
      241 tggcgagcac gagggtgtcc tcgatcgccg tcacctgcgc catgaaggga ccggtgcgga
      301 tcagggtcga catgccgaag gcggcacccg tgccacgccg gtcgaggaaa tcgccgttct
      361 cgtggtgcag gtctacggct ccggcgcgca ggatgaacat cgtgtcgttg tcctggccga
      421 ccgacaggat cctcgtcccg cggcggaagt agcggacctc gagcatggcg ggaagctcat
      481 cgagcagccg ggcgggcagc gtgtcgaagg gagggatccc ggcgacgaag tcccggacct
      541 cggccagctc ggcgttcatg gttccgcatc atggcatggc cccgggcaaa cggacgtgct
      601 gctctcagcc gcggcaggtg ggactctcgc atcgagtcgg ataccgtggg ggcgcctaca
      661 cgagtggaga cacaggaggg caccgggaca tgggtctgca cgagacgctc ctggcgcccg
      721 accgacgggc cgccacggtg gacgggttcg tcgccttcgt cgaggcggag gtggcaggca
      781 agtcgggact ggggggagcc gccgtcaaga ccgcctacgc cgccgcgcgg aagatcagcc
      841 cgacgatcgt gcggcgcgcc gtggccggga tgctgcccga cttcctcgac ggtttggacc
      901 ccttctgggc cgcccgcggc gagggcgcct tcagcgaggc gctcgggtcc aggccggccg
      961 aggtgacgga tgcactgctc agcgtcaccg accgccgcgc agccaacccc agacacgggg
     1021 cactgaccaa gatctatgct gcggtgcgac cccgagccgc acggcacgtc accgacgcgc
     1081 tccccgggct cgccaccacg gtcgagcggc tcatgggcta gcccctggtc agtcctggtc
     1141 agtcctggga ttggaccgag accggaccgg ccccggtcgt gagcaggatg agctcgcccg
     1201 cggcgtcggt ggcggccagg tcgacgaccg cgcggatccg ccagtcccgg tcgccggccg
     1261 ggtcgtcgat gatctgctcg acccgccagc ggcccggctc gggatggatg acggtgagcc
     1321 ccggcccacg cgcgtccggc ccggtcccca catcggcgta ctcggcgaag tagccctcga
     1381 ccgcctcctg ccagcccagc gcgtcgagtg gggacgcatc ccacggcgcc acgtcgatcg
     1441 gcgacaggtc cagcgcctcg cggccccgcg cgacggcggc ttcgtcgagc gcggccaggt
     1501 cgttccagcg gcgcagcgcg aagagggaga ccttgcgcca catggccgtt cgcaccatga
     1561 cccgcagcgc ccgctcgttg gcgctcagcg ggcgctcggc gccggcccgc gcggtcgcga
     1621 gggggtccag cccgcccggg gtgagctgac cgtcataccc actggtgagc tgctcccact
     1681 cctcgagcag gctggagtcg gtctggcgga cgacctcccc gagccactcg acgatgtcgg
     1741 tgagctcctc ggtgcgcgcg ctgaggggca cggtctggcg cagcgccttg taggcgtcgg
     1801 acaggtagcg cagcaccatg ccctcgccgc gggtcagctt gtagaacgcg atgaactcgc
     1861 cgaaggtcat ggcccgctcg tgcaggtcac gggcgaccga cttcggagac aggtcacgct
     1921 cggagatcca cggatgggta tggcggtaga gctccagcgc cgccgtgagc agctcctcga
     1981 gcggcttggg ccaggacacg tcgtcgagca gctccatccg ctcgtcgtag tcgagcccgt
     2041 cggccttcat ctcggcgacg gcctggccgc gggcgagcca ctgctgggcg ccgagcacgc
     2101 cccgcgggtc ctccagggtc gactcgatga cgctcacgac atcgagcgcg tacgacggcg
     2161 acgcggggtc gagcagctcg atcgcggcga gggcgaatgc ggacaggggc tggttgaggg
     2221 cgaacccgcg ctggaggtcc atggtcaggg cgaccgtccg gccggtctcg tccggctcgg
     2281 ggagccgctc gacgactccc gcagcgagca gcga
//
LOCUS       DATAIX010000257         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_13584, whole genome shotgun sequence.
ACCESSION   DATAIX010000257 DATAIX010000000
VERSION     DATAIX010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13584"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VHM65_03820"
     CDS             <1..930
                     /locus_tag="VHM65_03820"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX2704861.1"
                     /translation="GPELLPKDSPVRVFFSSLARDFPYASGPEVSVAAQGSLDEVTRW
                     AEGSVATLPGVLSARTEQRSPQIVAVSVYVDGAAQDQASRDVVASIRENRPAFQTWVA
                     GPAARLADFTASTASRAPFAGAVVALGTLALLFLLTGSLVIPLKALVLNLLSLGASLG
                     ILVWVFQQGHLEWALGFSSNGAIESFIPLLVLAFGFGLSMDYEVFLISRIAEIYHGGG
                     TTDDAVWHGLQRSGRIITSAALLIVIVFAGFIAGQLIVIKQTGLALTTAILLDASLVR
                     MLLVPATMTLLGEWSWWSPAPLRRWHAKYGIIE"
     gene            938..1702
                     /locus_tag="VHM65_03825"
     CDS             938..1702
                     /locus_tag="VHM65_03825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2704862.1"
                     /translation="MAAPGHLATYDALLAASGESPYIRYAVGARGVAAARAVPGGVAV
                     PRTTHTGRQSWTLLGDARAVGELLGQAPFQGAEAARLPFSLTLARTCLPLLDQGFRIT
                     AGDDWDWMWTLTSPPASPRAHAEQRDVIPLAGRDASAVARFLERHSPRASTQPGGDHD
                     WFGIHDASGDLVAIGALGTTSAGAGHLSSVAVDEHRRGEGLGARITAELTRIAVQRCG
                     ACTLGLYADNAPARALYRSVGYDGPLEWSTRIVTRR"
     gene            complement(1689..>1991)
                     /locus_tag="VHM65_03830"
     CDS             complement(1689..>1991)
                     /locus_tag="VHM65_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX2704863.1"
                     /translation="DMDIQRRIFKALGLTDEETEEKFGYFLEALSYGAPPHGGIAFGW
                     DRIVALLVGAESIRDVIAFPKSGGGYDPLTAAPAPITPEQRREAGVDAAPTGVTGG"
BASE COUNT          254 a          756 c          659 g          322 t
ORIGIN      
        1 ggccccgagc tgctccccaa ggacagcccc gtccgggtct tcttctcctc gctcgctcgc
       61 gacttcccct acgccagcgg tcccgaggtc tcggtcgcag cccagggatc cctggacgag
      121 gtgacgaggt gggccgaagg ctcggtcgcc acactcccgg gagtcctgtc ggcccggacc
      181 gagcagcgct caccgcagat cgtcgcggtg tccgtctacg tcgacggcgc ggcccaggac
      241 caggcgtccc gcgacgtcgt cgcctcgatc cgcgagaacc ggcccgcctt ccagacgtgg
      301 gtggccggac ccgcggcccg cctcgccgac ttcacggcct cgacggcttc ccgcgccccc
      361 tttgccggag ccgtcgtcgc cctcggcacg ctcgcgctgc tcttcctgct caccggctcg
      421 ctcgtcatac ccctcaaggc cctcgtcctc aacctgctct cgctcggcgc gtcgctgggc
      481 atcctcgtct gggtgttcca gcagggacac ctggagtggg cgctcggctt cagctccaac
      541 ggagccatcg agtcgttcat cccgttgctc gtgctcgcgt tcgggttcgg cctgtcgatg
      601 gactatgagg tcttcctcat ctcccgcatc gccgagatct accacggagg cgggaccacc
      661 gacgacgccg tctggcacgg gctgcagcga tccgggcgca tcatcacctc ggcggcgctg
      721 ctcatcgtca tcgtcttcgc cgggttcatc gccggccagc tcatcgtcat caaacagacc
      781 gggctcgccc tgacgacggc gatcctgctg gacgcctcac tcgtgcggat gctgctcgtt
      841 cccgccacga tgacgctgct cggtgaatgg tcgtggtggt cgccggcacc cctgcggcgc
      901 tggcacgcca agtacggcat catcgagtag gtcaccggtg gctgcacctg gacacctcgc
      961 cacgtatgac gcgctcctcg ccgcgagcgg ggagagcccc tacatccggt atgccgtcgg
     1021 tgcgcggggc gttgcggccg ctcgggccgt gcccggcggc gtggcagtgc ccaggacgac
     1081 ccacaccggg aggcagtcgt ggaccctgct cggcgacgcc cgcgcggtcg gcgagctgct
     1141 cgggcaggca cccttccagg gcgcagaggc cgcccgtctc ccgttctcgc tcaccctggc
     1201 gcgcacctgc ctgccgttgc tggaccaggg ttttcgcatc acggctggcg acgactggga
     1261 ctggatgtgg accctgacct caccccccgc ctcgcccagg gcgcacgccg agcagcggga
     1321 cgtcataccc ctggctggac gggacgcctc ggccgtcgcg aggtttctcg agcggcactc
     1381 cccgagggcg tccacccagc cgggcgggga ccacgactgg ttcggcatcc acgacgccag
     1441 cggcgacctc gtcgctatcg gagcgctcgg caccacctcg gctggggcgg ggcacctgtc
     1501 ctccgtcgcc gtggacgagc atcgccgggg cgagggactg ggtgcccgga tcaccgccga
     1561 gctgacccgg atcgcagtcc agcgctgcgg cgcctgcacc ctcggcctct atgccgacaa
     1621 tgcaccggcc cgcgcgctct accgctcggt ggggtacgac gggcccctcg agtggtcgac
     1681 gcgcatcgtc acccgccggt gacgcccgtc ggcgcggcgt cgacgccggc ctccctgcgc
     1741 tgctcgggcg tgatcggggc cggcgccgcc gtgagcgggt cgtagccgcc accggacttc
     1801 gggaaggcga tgacgtcgcg gatcgactcg gcgccgacga gcagcgcgac gatgcggtcc
     1861 cagccgaagg cgatgccacc gtgcggcggt gcgccatagc tcaaggcctc gaggaagtag
     1921 ccgaacttct cttcggtctc ctcgtccgtc aaaccgagcg ccttgaagat ccggcgctgg
     1981 atgtccatgt c
//
LOCUS       DATAIX010000258         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_214491, whole genome shotgun sequence.
ACCESSION   DATAIX010000258 DATAIX010000000
VERSION     DATAIX010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214491"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VHM65_03835"
     CDS             complement(<1..189)
                     /locus_tag="VHM65_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704864.1"
                     /translation="MLSHPKGVRPRLLAVVAVLVALAGWVVQRLAGGRTQLATAGWLT
                     VILLAAMAVVVYAAGIPVR"
     gene            complement(200..763)
                     /gene="folK"
                     /locus_tag="VHM65_03840"
     CDS             complement(200..763)
                     /gene="folK"
                     /locus_tag="VHM65_03840"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX2704865.1"
                     /translation="MSIRRERAVPVVVALGANLGDPRATLAAAVRDLGALEGLSRLAV
                     SDLVETDPVGGPGQPPYLNAVATAVVSLGPGPLLAALHEIEARHGRVRDVRWGARTLD
                     LDLVQYGRPGERDEVIRAGPALTLPHPRAPERAFVLVPWAQVDPSATARVGDAIREVA
                     QIAAVTDRSGVRPGPSWPARPDAWRQR"
     gene            806..>1542
                     /gene="folB"
                     /locus_tag="VHM65_03845"
     CDS             806..>1542
                     /gene="folB"
                     /locus_tag="VHM65_03845"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX2704866.1"
                     /translation="MDRHRDGPDEVTTHHVGGDLLGVRGYGYHGVLADEKERGQEFLV
                     DVVLELSTRAAGLTDDLSLTVSYAEVADQVVTRIAGPSVDLIETLAEQVATDVLGRDL
                     VRAVTVTVHKPQAPVGHPFGDVQVSIRRERAVPVVVALGANLGDARATLVGAVRDLGA
                     LDGLSRLAVSDLVETDPVGGAGQPFYLNAVAAAVTSLGPGPLLAALHEIEARHGRVRD
                     VRWGARTLDLDLVQYGRPGERDEVIRN"
BASE COUNT          204 a          571 c          572 g          195 t
ORIGIN      
        1 gcgcaccggg atcccggccg cgtagacgac gaccgccatg gcggcaagga gtatgacggt
       61 cagccagccg gcggtggcca gctgcgtcct ccccccggca aggcgctgca cgacccaccc
      121 ggccagcgcg acgaggaccg cgacgacggc gagcagtcgc ggacgaactc ctttgggatg
      181 gctcagcatt cgtgctccgt catcgctgtc tccaagcatc gggccgggct ggccacgatg
      241 gtcccgggcg cacacccgac cggtcggtca cagccgcaat ctgcgctact tcgcgtatgg
      301 cgtcacccac ccgtgcggtc gcgctcgggt ccacctgcgc ccagggcacg agcacgaagg
      361 cccgctcggg cgcccgggga tgcgggagcg tcagggccgg gccggcccgg atcacctcgt
      421 cgcgctcccc cggacggccg tactgtacga ggtcgaggtc gagcgtccgg gcaccccagc
      481 gcacatcgcg cacccgcccg tggcgcgcct cgatctcgtg cagggccgca agcaaggggc
      541 ccgggccgag actgacgacg gcggtcgcga ccgcgttgag gtagggcggc tgcccgggcc
      601 ccccgaccgg gtcggtctcg acgaggtccg acacggcgag ccgcgacagc ccctcgaggg
      661 cgccgaggtc cctgaccgcg gcggccaggg tggcccgcgg gtcaccgagg ttggcgccga
      721 gcgcgacgac gaccggcacg gcccgctctc ggcggatgct cacctggacg tccccgaacg
      781 gatggcccac cggggcctgc ggcttgtgga ccgtcaccgt gacggcccgg acgaggtcac
      841 gacccatcac gtcggcggcg acctgctcgg cgtccgcggc tacggctacc acggggtgct
      901 cgcggacgag aaggagcgcg ggcaggagtt cctcgtcgac gtcgtcctcg agctgtcgac
      961 ccgggccgcg ggcctcaccg acgacctgtc cctcacggtg agctacgccg aggtggccga
     1021 ccaggtcgtg acccggatcg ccgggccgtc ggtcgacctc atcgagaccc tcgccgagca
     1081 ggtggccacc gacgtgctgg gccgtgacct cgtccgggcc gtcacggtga cagtccacaa
     1141 gccgcaggcc ccggtgggcc atccgttcgg ggacgtccag gtgagcatcc gccgcgagcg
     1201 ggccgtgccg gtcgttgtcg cgctcggcgc caacctcggt gacgcgcggg ccaccctggt
     1261 cggcgcggtc agggacctcg gcgccctcga tgggctgtcg cggctcgctg tgtcggacct
     1321 cgtcgagacc gacccggtcg ggggggccgg gcagccgttt tacctcaacg cggtcgcggc
     1381 cgcggtgacg agtctcggcc ccggcccctt gcttgcggcg ctgcacgaga tcgaggcgcg
     1441 ccacgggcgg gtgcgcgacg tgcgctgggg tgcccggacg ctcgacctcg acctcgtgca
     1501 gtacggccgt ccgggcgagc gcgacgaggt gatccggaac ga
//
LOCUS       DATAIX010000259         2323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_44001, whole genome shotgun sequence.
ACCESSION   DATAIX010000259 DATAIX010000000
VERSION     DATAIX010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44001"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VHM65_03850"
     CDS             complement(<1..520)
                     /locus_tag="VHM65_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2704867.1"
                     /translation="MTSYDVVVVGGGIVGVASARALLRARPGLRVLLVEKEHRLAAHQ
                     SGHNSGVIHAGVYYAPGSLKARLCREGAAATLALCEEHGLPLRRTGKLIVATGACELG
                     RLLALGNRAASSGIAVRRVFAPELRELEPHVNGLAALLVPGSASVDYRLVTKTLGAEI
                     EGRGAQVVTGRRV"
     gene            complement(517..1479)
                     /locus_tag="VHM65_03855"
     CDS             complement(517..1479)
                     /locus_tag="VHM65_03855"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEX2704868.1"
                     /translation="MSTMTRVAAVDCGTNSIRLLIAAVDSSGALHDIVRRMEIVRLGH
                     GVDRTGVIEPAAMARTLAMAREYAAQCVDHEVEAVRFVATSASRDARNAGEFIEGVTT
                     AFARFGTAPEVVPGDVEAELSFTGATGDAQAAGLPGPYLVVDIGGGSTELVRGFTRVE
                     QKVSVDVGCVRLTERRLHSDPPTEDEIRLAVADIDEAIDLAMGSVDLAGVGTLIGLAG
                     SITTITAYALDLPSYQPERVHLSRLPVADVQRACADLLHRTRAGKAAVPSLHPGRVDV
                     IGGGALIWSRVIDRLVETSGVTEVITSEHDILDGIALSVVAPHA"
     gene            complement(1479..2009)
                     /locus_tag="VHM65_03860"
     CDS             complement(1479..2009)
                     /locus_tag="VHM65_03860"
                     /inference="COORDINATES: protein motif:HMM:NF016314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HEX2704869.1"
                     /translation="MSTASQPTPDDLIAVGRQLGRPPRGVVGVAHRCPCGEPDVVRTA
                     SRLPDGTPFPTTFYATCPRLTGAVSTLEASGMMREMSARLAGDPVVAQGYRRAHSDYL
                     RRRSELGEVAETAGVSAGGMPARVKCLHALVAHALAAGAGVNPFGDEALAALPQWWRP
                     QGCAERMGQKATEEAP"
     gene            complement(2006..>2323)
                     /locus_tag="VHM65_03865"
     CDS             complement(2006..>2323)
                     /locus_tag="VHM65_03865"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX2704870.1"
                     /translation="SSDIAALRERVAAQSRTVEQLTAEQAKWSDAAHVEQQARERLKF
                     VRVGDRSYTVIDPVPDASGAQGAPVVSAASVNGAAPWYGQLWQSVLVADQPSAGLTRG
                     RAK"
BASE COUNT          327 a          869 c          827 g          300 t
ORIGIN      
        1 tcacccggcg gccggtgacg acctgcgctc cccggccctc gatctcggcg ccgagggtct
       61 tcgtcaccaa ccggtagtcg acgctcgccg agccggggac gagcagcgcc gccagcccgt
      121 tcacgtgggg ctcgagctcg cgcagctcgg gagcgaagac gcgacgcacc gcaatgccgc
      181 tcgatgccgc ccggttgccg agggcgagca gccgccccag ctcgcaggca cccgtcgcga
      241 cgatgagctt gccggtccgg cgcaggggca gcccatgctc ctcgcagagc gcgagcgtcg
      301 ccgcggcccc ctcgcggcac agccgggcct tgagcgaccc gggcgcatag tagaccccgg
      361 cgtggatcac gccgctgttg tgcccgctct ggtgagcggc gaggcggtgc tccttctcga
      421 cgagcaggac acgcagcccc ggacgtgcgc ggagcagggc ccgggcgctc gccaccccga
      481 cgatgccccc gcccacgacg acgacgtcgt atgacgtcac gcgtgtggcg ccaccacact
      541 cagcgcgatg ccgtcgagga tgtcgtgctc ggaggtgatg acctcggtga caccgctggt
      601 ctcgaccagc cggtcgatga cccgggacca gatgagcgcc ccgccgccga tgacgtcgac
      661 ccggcccggg tgcagggacg gcactgccgc cttgccggcc cgggtccggt ggagcaggtc
      721 ggcgcaggct cgctgcacgt ccgcgacggg aaggcgggac aggtggacgc gctcgggctg
      781 gtaggacggc aggtcgagcg catacgcggt gatcgtcgtg atactgccgg ccaggccgat
      841 gagcgtgccc acaccggcga ggtcgacgct gcccatcgcg aggtcgattg cctcgtcgat
      901 atcggcaacg gccagccgga tctcgtcctc ggtcggcggg tcgctgtgca ggcgccgctc
      961 ggtgagccgg acgcatccga catcgacgga gaccttctgc tccacccggg tgaagccgcg
     1021 gacgagctcg gtcgagccgc cgccgatgtc gacgacgaga tagggcccag gcagcccggc
     1081 cgcctgggcg tccccggtgg ctccggtgaa ggagagctcg gcctcgacat ccccggggac
     1141 gacctcggga gccgtgccga agcgggcaaa cgccgtggtg acgccctcga tgaactcgcc
     1201 tgcgttgcgc gcgtcccggg aggccgaggt cgcgacgaac cgcaccgcct cgacctcgtg
     1261 gtcgacgcac tgggcggcat actcacgggc catcgccagg gtccgggcca tcgccgccgg
     1321 ctcgatcact cccgtccggt ccacgccgtg gcccagacgc acgatctcca tccggcgcac
     1381 gatgtcgtgc agcgcgccgg aggagtcgac cgccgcgatg agcagccgga tcgagttcgt
     1441 cccgcagtcg accgccgcga cccgcgtcat ggtggacatc acggtgcctc ctccgttgcc
     1501 ttctggccca tcctctcggc acacccctgc gggcgccacc actggggcag cgcggcaagg
     1561 gcctcgtccc cgaacgggtt gacccccgcc ccggcggcca gcgcgtgagc gacgagcgca
     1621 tgcaggcact tgactcgcgc cggcatcccc ccggccgaca caccagcggt ctcggcgacc
     1681 tcgccgagct cgctccggcg cctgaggtag tccgagtggg cgcgccggta gccctgtgcg
     1741 acaacgggat cgccggccag gcgcgccgac atctcgcgca tcatccccga ggcctccagg
     1801 gtgctcaccg ccccggtgag acgtgggcag gtggcataga acgtcgtcgg gaacggagtc
     1861 ccgtcgggca gtcgcgaggc ggtgcgcacg acatccggct ccccgcaggg gcaccgatgg
     1921 gcgaccccga ccacgccccg gggaggacgg ccgagctggc gccccacggc gatgaggtcg
     1981 tccggagtgg gctgcgaggc ggtgctcact tcgcccgccc ccgggtcagg ccggcgctgg
     2041 gctggtcggc aacgaggacc gactgccaga gctggccgta ccacggggcc gcaccgttga
     2101 ctgacgccgc ggacacgacc ggagcgccct gggcgcccga cgcatccggc accgggtcga
     2161 tcaccgtgta ggaccggtcc cccacccgga cgaacttgag gcgctcccgg gcctgttgct
     2221 cgacatgcgc ggcgtcggac cacttggcct gctccgcggt gagctgctcc accgtcctgc
     2281 tctgtgcggc cacccgctcg cgcagggcgg ctatgtccga gct
//
LOCUS       DATAIX010000260         4720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_73949, whole genome shotgun sequence.
ACCESSION   DATAIX010000260 DATAIX010000000
VERSION     DATAIX010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73949"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="VHM65_03870"
     CDS             complement(<1..1241)
                     /locus_tag="VHM65_03870"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704871.1"
                     /translation="MPGHPPTVVYVPGDAAAVSVGADEVAAALAARGEVTVIRNGSRG
                     MLWLEPLVEVVTDRGRVGYPNVSVAALDGLVAAGLLDGAEAGGCLGAVGELPWLARQR
                     RVTFARVGIVDPASVEDYAGHGGWAGLRAALALAPTAVIDEVIASGLRGRGGAGFPTG
                     VKWRTVHDARSDVKFVCANADEGDSGTFADRMLMEGDPFTLVEGMTIASYAVGAREGF
                     VYIRSEYPHAVTAMRRAVGAARAHGFLGPDILGSGHSFELEVRVGAGAYICGEETSML
                     ESLEGRRGEVRAKPPIPALQGLFGEPTIVNNVLSLATVPMILADGAATYASMGVGRSR
                     GTQVCQLAGNIARGGIVETGFGLTLGELVEGYGAGTRSGRPVRAVQVGGPLGAYLPAE
                     RLDLAMDYEAFAAADAMVGHGG"
     gene            complement(1247..1714)
                     /locus_tag="VHM65_03875"
     CDS             complement(1247..1714)
                     /locus_tag="VHM65_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX2704872.1"
                     /translation="MTLLPPPASTTGRTQRVREIASGHLGRRGPLLPILHGVTDDLGH
                     LEREDIETIADVLNLSRAEVHGVVSFYSDFRRSPAPAHAVTLCRAEACQAVGAEGLYA
                     DTEATFAGRADVEVSHVFCLGNCALGPSGTVDGRLHGRLSRDRLAELTTGWQG"
     gene            1968..2237
                     /gene="rpsO"
                     /locus_tag="VHM65_03880"
     CDS             1968..2237
                     /gene="rpsO"
                     /locus_tag="VHM65_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006214488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX2704873.1"
                     /translation="MPLDAAVKKQIMTDYATVEGDTGSPEVQVAMLTQRIKDLTEHSR
                     THKHDHHSRRGLLLLVGRRKRMLRYLEATDIERYRALIKRLGLRR"
     gene            2389..4626
                     /locus_tag="VHM65_03885"
     CDS             2389..4626
                     /locus_tag="VHM65_03885"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX2704874.1"
                     /translation="MEGPDIQFAEAVIDNGAFGTRTVRFETGRLAKQANGSVTAYLDD
                     DTMLLATAAAGKTPKDHFDFFPLTVDVEERMYAIGKIPGSFFRREGRPSTDAILTCRL
                     IDRPLRPSFTKGLRNEVQIIISVFALNPDHQYDVVAINAASLATQISGLPFSGPIGGV
                     RVALIDGQWVGFPNFSDAERSVFDMVVAGRIVTRSDGSEDVAIMMVEAEATDSTWDLV
                     KHQGKAAPTEEVVAAGLEAAKGFIATLCHAQSALAAGASKDRVEFPVFLDYEQDAYDA
                     VEASTADDLSAALQIPGKTEREDRLDEIKFRVKGELADAFDGREKEISAAYRAVQKKL
                     VRQRILRDGVRIDGRGLRDIRALSAEVEVLPRVHGSAIFERGETQIMGVTTLNMLKLE
                     QQLDTLSPVTRKRYMHNYNFPPYSTGETGRVGSPKRREVGHGALAERAIMPVLPTREE
                     FPYAIRQVSEALASNGSTSMGSVCASTLSLLNAGVPLRAPVAGIAMGLVSDEVDGETR
                     YAALTDILGAEDAFGDMDFKVAGTKEFITAIQLDTKLDGIPASVLAGALTQAREARLH
                     ILDVMAEAIDMPDEMSPYAPRIITVKVPVDKIGEVIGPKGKMINQIQDDTGADISIED
                     DGTVYIGAVDGPSAEAARAAINAIANPQMPEVGERFLGTVVKTTTFGAFISLLPGKDG
                     LLHISEVRKLVGGKRIDSVDDVVKIGQKIQVELKEIDPRGKLSLAAVMAEPSGEAFPG
                     GPAEA"
BASE COUNT          756 a         1703 c         1638 g          623 t
ORIGIN      
        1 ccgccgtgac cgaccatcgc gtcagctgcc gcgaacgcct cgtagtccat ggccaggtcg
       61 agccgctcgg cgggcaggta ggccccgagc ggtccgccga cctgcacggc ccgcaccggc
      121 cgcccggacc gcgtgccggc gccgtacccc tcgacgagct cacccagggt gaggccgaag
      181 ccggtctcga cgatgccgcc ccgggcgatg ttgccggcga gctggcagac ctgggtgccc
      241 cgcgaccggc ccacccccat ggaggcgtat gtcgcggcgc cgtccgcgag gatcatcggg
      301 accgtcgcga gggagagcac gttgttgaca atcgtcggct cgccgaagag cccctgcagg
      361 gcggggatgg gcggtttggc gcgcacctcg ccgcggcggc cttcgaggct ttccagcatc
      421 gaggtctcct cgccgcagat gtaggcgccg gccccgaccc ggacctccag ctcgaaggag
      481 tgaccgctgc cgaggatgtc cgggccgaga aacccgtgcg cgcgagcggc cccgaccgcc
      541 cggcgcatcg ccgtgaccgc gtgggggtac tccgagcgga tgtagacgaa cccctcgcgg
      601 gcgccgacgg cataggaggc gatcgtcatc ccctcgacga gcgtgaacgg gtcgccctcc
      661 atgagcatgc ggtcggcgaa cgtgcccgag tcgccctcgt cggcgttggc gcagacgaac
      721 ttcacgtccg accgagcgtc gtggaccgtg cgccacttga ccccggtcgg gaagcccgcg
      781 ccgccgcggc cgcgcagccc ggaggcgatg acctcgtcga tgaccgcggt cggcgccagc
      841 gcgagggcag cccgcagccc ggcccagccg ccatggccgg cgtagtcctc gaccgaggcc
      901 gggtcgacga tcccgacccg ggcgaaggtg acccggcgct ggcgggcgag ccaggggagc
      961 tcgcccacgg cgccgaggca gccgccggcc tcggcaccgt cgagcagccc agcggccacg
     1021 agcccgtcga gcgcggcgac gctgacgttg gggtagccca cccggccccg gtcggtgacg
     1081 acctcgacga ggggttcgag ccacagcatc ccccgagacc cgttgcgtat gacggtcacc
     1141 tcacctcgcg cggccagtgc ggcggccacc tcgtcggcgc cgaccgacac cgcggcggcg
     1201 tcgcccggga cgtagaccac ggtcggcggg tggcccggca taccactcat ccctgccacc
     1261 ccgtggtgag ctcggccagg cggtcgcgcg acagccggcc gtgcaggcgc ccgtcgacgg
     1321 tgccggacgg gccgagggcg cagttgccga ggcagaagac atggctgacc tcgacgtcgg
     1381 cccgacccgc gaaggtggcc tcggtgtcgg cgtagagacc ctcggcaccc acggcctggc
     1441 acgcctcggc gcggcacagc gtcacggcgt gggccggggc gggggagcgg cggaagtcgc
     1501 tgtagaacga cacgaccccg tggacctcgg cccgggacag gttgagcacg tcggcgatgg
     1561 tctcgatgtc ctcccgctcg aggtggccga ggtcatcggt gaccccgtgc aggatcggca
     1621 gaagcgggcc ccggcgcccc aggtggcccg acgcgatctc ccggacgcgt tgggtgcggc
     1681 cggtggtgct ggctggcggg ggaaggagcg tcatgcgtgc cttctcgggc gtaggtggcg
     1741 atcgggtgcg cgacctcaag gggggcggtg tgaggtaggt cgcagtctac gtcgccgcgt
     1801 tggacctgtc tggaggccgc cggcgtcggt gaagcggtgg attagggcct ggtccggcgc
     1861 gctggtaatc tcatgcgtgc cgtgatatcg gccgcggatc cacagagccc gggtgaacca
     1921 gccccgcagc gccgcgcaac gagaagccag aaggagcccc gacgcccatg ccgttggacg
     1981 cagctgtcaa gaagcagatc atgaccgact acgccacggt cgagggcgac accggttccc
     2041 ccgaggtcca ggtcgcgatg ctcacgcagc gcatcaagga cctcaccgag cactcgcgca
     2101 cgcacaagca tgaccaccac agccgccgtg ggctgctgct gctggtcggc cgtcgcaagc
     2161 gcatgctgcg ctacctcgag gccaccgaca tcgagcgcta ccgtgctctg atcaagcggc
     2221 tcggcctgcg ccgataacgc gtccgactgg ggcggtcccc gcgctgccgg ggaccgcccc
     2281 agtcgtatga gcacgaccca gcaccaccac gaccccgcac caccaggaca aacccgtgca
     2341 caacgacgat gcacgtggtc gacagagaaa aagaaagtga gggcccacat ggagggtcca
     2401 gatatccagt tcgccgaagc cgtcatcgac aatggcgcgt tcggcacccg cacggtccgg
     2461 ttcgagaccg gacgcctggc caagcaggcc aacggctccg tcaccgccta cctcgatgac
     2521 gacacgatgc tgctggcaac cgccgcagca ggcaagaccc cgaaggacca cttcgacttc
     2581 ttcccgctga cggtcgacgt cgaggagcgg atgtacgcga tcgggaagat tcccggctcg
     2641 ttcttccgcc gcgagggccg cccgagcacc gacgccatcc tcacctgccg cctcatcgac
     2701 cggccgctgc gcccgtcgtt caccaagggc ctgcgcaacg aggtccagat catcatctcg
     2761 gtgttcgccc tgaaccccga ccaccagtac gacgtcgtcg cgatcaacgc ggcctcgctg
     2821 gcgacgcaga tctcgggcct gccgttctcc ggcccgatcg gtggcgtgcg cgtcgcgctc
     2881 atcgacggcc agtgggtcgg cttcccgaac ttctccgacg ccgagcgctc ggtgttcgac
     2941 atggtcgtcg ccgggcgcat cgtcacccgg tccgacggct ccgaggacgt cgcgatcatg
     3001 atggtggagg ccgaggcgac cgactcgacg tgggacctgg tcaagcacca gggcaaggcc
     3061 gccccgaccg aagaggtcgt cgccgccggg ctcgaggccg ccaagggctt catcgccacg
     3121 ttgtgccacg cgcagagcgc gctcgcggcc ggcgcctcga aggaccgcgt cgagttcccc
     3181 gtcttcctcg actacgagca ggacgcctat gacgcggtcg aggcctcgac cgcggacgac
     3241 ctcagcgcgg ccctgcagat ccccggcaag accgagcgcg aagaccgtct cgacgagatc
     3301 aagttccggg tcaagggcga gctggcggat gcgttcgacg gccgcgagaa ggagatctcg
     3361 gcggcctacc gggccgtgca gaagaagctg gtccgccagc ggatcctgcg cgacggcgtc
     3421 cggatcgacg gccgcgggct gcgcgacatc cgggccctct cggccgaggt cgaggtgctg
     3481 ccgcgggtgc acggctcggc gatcttcgag cgcggcgaga cccagatcat gggggtgacg
     3541 acgctgaaca tgctcaagct ggagcagcag ctggacaccc tctcgccggt gacccgcaag
     3601 cgctacatgc acaactacaa cttcccgccc tactcgaccg gcgagaccgg ccgggtaggc
     3661 tcgccgaagc ggcgcgaggt gggccacggc gcgctcgccg agcgggccat catgcccgtc
     3721 ctgccgaccc gcgaggagtt cccctacgcg atccgccagg tgtccgaggc gctggcctcc
     3781 aacgggtcga cctcgatggg ctcggtctgc gcctctaccc tgtccctgct caacgccggt
     3841 gtgccgctgc gcgccccggt cgcgggcatc gccatgggcc tcgtctccga cgaggtcgac
     3901 ggggagaccc gctatgccgc gctcaccgac atcctcggcg ccgaggacgc gttcggcgac
     3961 atggacttca aggtcgccgg caccaaggag ttcatcaccg cgatccagct cgacaccaag
     4021 ctcgacggca tccccgcctc cgtgctcgcc ggcgcgctga cacaggcccg cgaggcccgg
     4081 ctgcacatcc tcgacgtcat ggccgaggcc atcgacatgc ccgacgagat gtcgccgtac
     4141 gccccgcgga tcatcacggt caaggttccg gtcgacaaga tcggcgaggt catcggcccg
     4201 aagggcaaga tgatcaacca gatccaggac gacacgggcg ccgacatctc gatcgaggac
     4261 gacggcaccg tctacatcgg cgcggtcgac ggcccgtcgg ccgaggcggc ccgggcggcg
     4321 atcaacgcga tcgccaaccc gcagatgccc gaggtcgggg agcgcttcct cggcaccgtc
     4381 gtcaagacga cgaccttcgg ggcgttcatc tcgctgctgc ccggcaagga cggcctgctg
     4441 cacatctccg aggtgcgcaa gctcgtcggg ggcaagcgga tcgactcggt cgacgacgtc
     4501 gtcaagatcg gccagaagat ccaggtcgag ctcaaggaga tcgacccgcg cggcaagctc
     4561 tcgctcgcgg cggtcatggc cgagccttct ggtgaggcct tcccgggcgg tcccgcggag
     4621 gcctgagcct gcgcgcacca gcggcgagcg gtccgtcata ccacgtcagt gggtatggcg
     4681 ggccgctcgc cgttctgccg cggaccaacc gaaccgagtg
//
LOCUS       DATAIX010000261         1739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_125258, whole genome shotgun sequence.
ACCESSION   DATAIX010000261 DATAIX010000000
VERSION     DATAIX010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125258"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VHM65_03890"
     CDS             complement(<1..531)
                     /locus_tag="VHM65_03890"
                     /inference="COORDINATES: protein motif:HMM:NF023917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3710 domain-containing protein"
                     /protein_id="HEX2704875.1"
                     /translation="MAIFRRNRREGDASAVDRAPADRDLDEPDATESADPADAPDAAD
                     APEPVVDRADGPFDAAEADDAERLDLGALRIVGAPGMHLQLELDESTQAVVSATVTVG
                     GSAVQLQAFAAPRTTRIWPEIRQEIAASLVTGGGTADVVDGPLGRELMGRMPGRGADG
                     RTAFSPVRFLGIDGPRW"
     gene            complement(542..1027)
                     /gene="dut"
                     /locus_tag="VHM65_03895"
     CDS             complement(542..1027)
                     /gene="dut"
                     /locus_tag="VHM65_03895"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX2704876.1"
                     /translation="MDHAPPITVLLRRLDADLPVPAYALPGDAGVDLFAREGVTLLPG
                     RRALVPTGVSIALPHGYAAFTHPRSGLAARHGVTLVNSPGTVDSGYRGEIMVNLVNLD
                     PAAPFEIRRGDRVAQLVVQQVVTVEFALVDSLPESVRGETGHGDSGGFGPAAAQTLDA
                     Q"
     gene            complement(1044..1343)
                     /locus_tag="VHM65_03900"
     CDS             complement(1044..1343)
                     /locus_tag="VHM65_03900"
                     /inference="COORDINATES: protein motif:HMM:NF025204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 domain-containing protein"
                     /protein_id="HEX2704877.1"
                     /translation="MATDYDAPRKTDDDLSEDSLEELKARRVDTKSSSVDIDEAEQAE
                     GFELPGADLSGEELSVRVLPRQADEFTCSRCFLVHHRSQLAKEVGGQMICRECAA"
     gene            complement(1458..>1739)
                     /locus_tag="VHM65_03905"
     CDS             complement(1458..>1739)
                     /locus_tag="VHM65_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX2704878.1"
                     /translation="VLTQVLPLVRDIRRLGSAALDLCLVAQGVVDAFYEDLLNPWDVA
                     AGLLIVAEAGGIVSGWTPAEPALPAVVAGSPAIHTALCEVLNRVRGAPM"
BASE COUNT          250 a          608 c          633 g          248 t
ORIGIN      
        1 ccaccgcggg ccgtcgatgc cgaggaagcg caccggcgag aaggccgtgc ggccatcggc
       61 cccccggcca ggcatccgtc ccatgagctc gcgtccgagg ggaccgtcga cgacgtcggc
      121 ggtgcctcct cccgtaacga ggctcgccgc gatctcctgg cggatctccg gccagatccg
      181 ggtggtccgt ggtgcggcaa aggcctgcag ctggaccgcc gagcccccca ccgtcacagt
      241 ggcgctgacg accgcctggg tggactcgtc gagctcgagc tgaaggtgca tcccgggggc
      301 gccgacgatg cgcagcgccc cgaggtcgag gcgctcggcg tcgtcggcct cggccgcgtc
      361 gaaggggccg tccgcacggt caaccactgg ctccggcgcg tcggccgcgt ccggcgcgtc
      421 ggccgggtcg gcggactcgg tggcatccgg ctcgtcgagg tcccggtcgg ccggcgcacg
      481 gtcgactgcg gaggcgtcgc cttcccggcg gttgcgacgg aagatcgcca ctggctgtcc
      541 ttcactgtgc gtcgagggtc tgcgccgcag ccgggccgaa gccgccactg tcgccgtgcc
      601 cagtctcccc ccgaacgctc tccgggagcg aatccacgag ggcgaactcg acggtgacca
      661 cctgctggac gaccagctgg gcgacccggt cgcctcggcg gatctcgaac ggcgccgccg
      721 ggtccaggtt gaccaggttg accatgatct cgccccggta gcccgagtcg accgtcccgg
      781 gagagttgac gagggtgacc ccgtgccggg ccgccagccc ggagcggggg tgggtgaacg
      841 cggcataccc gtgagggagc gcgatgctga caccggtggg cacgagtgcc cggcgaccgg
      901 ggagcagcgt gaccccctca cgggcgaaga ggtccacgcc cgcgtcgccg gggagtgcgt
      961 acgcggggac cggcaggtcg gcgtcgaggc gccggagcag gacggtgatc ggcggggcgt
     1021 gatccacggc tggtgtcagt ggcctaggcc gcgcactcgc ggcagatcat ctgaccgccg
     1081 acctccttgg ccagctggct gcggtggtga accaggaagc acctggagca ggtgaactcg
     1141 tcggcctggc gcgggaggac gcgcacggag agctcctcac cggacaggtc cgcccctggg
     1201 agctcgaagc cttcggcctg ctcggcctcg tcgatatcga cactcgagga cttcgtatcg
     1261 actcgtcgcg ccttgagctc ctcgagcgag tcctccgaca ggtcgtcgtc ggtcttcctg
     1321 ggcgcgtcgt agtcagtcgc catcgtcgtg ctctctcccg tctcgtgtgc acatcatgga
     1381 tgcgtgccgg gtcaaacgca tgggcaggac gtattgtgcc cctgcgcggc ggattttgcc
     1441 tcatccgcgc cgcagagtca catcggggct ccgcggaccc ggttgaggac ctcgcacagc
     1501 gcagtgtgaa tcgccgggga gcccgccacg acagcgggca gggcaggctc ggccggtgtc
     1561 cacccggaga ctatgccacc ggcttcggcg acgatgagca ggcctgcagc gacatcccac
     1621 gggttgagca gatcctcgta gaacgcgtcg acgacaccct gcgccacgag gcacagatcc
     1681 agggcggcgc tgccgagccg gcggatgtcc cgcacgagcg gcaggacctg cgtcagcac
//
LOCUS       DATAIX010000262         1607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_244459, whole genome shotgun sequence.
ACCESSION   DATAIX010000262 DATAIX010000000
VERSION     DATAIX010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244459"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="VHM65_03910"
     CDS             <1..1026
                     /locus_tag="VHM65_03910"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HEX2704879.1"
                     /translation="EWTGFAALGALSRVGCRAFAPSGSPGAGGPRPSAQHPEPSWAAD
                     MARDLPATVDHIVLQADLTALAPGPLEPDLAALMRTVARVESRGTATVFRIDHASIRS
                     ALDTGRSADELLAALADASRTPVPQPLDYLIRDIARVHGRTRIGGATAYVRSDDEATL
                     RAMVSHRVLAPLRLRLLAPSLAVSSAEPLTVLELLREAGFAPVQETLDGAVVIQTPKS
                     KRTPSRRRPAPMPVSGLDPAAAAALVRQLREAEGSALTLRAHGGNPRTGLPPTSDPLV
                     VLAALRDATAEGRGVWIGYTDQNGTASPVFFYPSRIEAGRAWGAVPAAREDRGFLLHR
                     ITGVVAS"
     gene            complement(1023..>1607)
                     /locus_tag="VHM65_03915"
     CDS             complement(1023..>1607)
                     /locus_tag="VHM65_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX2704880.1"
                     /translation="ARKNLNRSVAESVTMFEPVVARARAEGLWVRAYVSMCFGDPWEG
                     DVPVGQVVEVARRLMDLGCDELSIGDTIGVGTTGHVHRLLAALAAAGIGLDRVAVHFH
                     DTYGQALANTMAALADGVTVVDASAGGLGGCPYAKSATGNLATEDLVWALHGAGVETG
                     VDLEALVETSAWLARQLGRPSPSRVVRALAGGAA"
BASE COUNT          206 a          619 c          570 g          212 t
ORIGIN      
        1 gagtggacgg gcttcgccgc gctgggcgcg ctgagccggg tgggttgtcg ggcgttcgcg
       61 ccgtccggat cgcccggtgc cggcgggcca cgtccctcgg cccagcatcc cgagccgtcg
      121 tgggcggccg acatggcccg cgacctcccg gcgaccgtcg accacatcgt gctccaggcc
      181 gacctcacag ccctggcgcc cggaccgctc gaacccgacc tcgcagccct gatgcggacc
      241 gtcgcgcgag tcgagtcacg aggcaccgca acggttttcc gcatcgacca tgcgagcatc
      301 cgcagcgccc tcgacacggg ccggtccgcc gacgagctcc tcgcggcact cgccgacgcg
      361 agccggaccc cagtcccgca gccgctggac tacctcatcc gcgatatcgc ccgggtccac
      421 ggccggacgc ggatcggcgg cgcgacggca tacgtccgct cggacgacga ggcgacgctg
      481 cgtgcgatgg tgagccaccg cgtcctggca cccctgcggc tgcgtctgct ggcaccgtcc
      541 ttggcggtct cctcggccga gcccctcacg gtcctcgagc tgctccgcga ggcggggttc
      601 gcccctgtcc aggagaccct cgacggcgcc gtcgtcatcc agactcccaa gtccaagcgg
      661 actccgtccc gacgccggcc ggcgccaatg ccggtctccg gcctggaccc ggcggcagcc
      721 gccgcgctcg tgcgtcagct tcgtgaggcc gaagggtcgg cgctcacgtt gcgggcgcat
      781 ggcgggaacc cgcggaccgg gctgcccccg accagcgacc cgctcgtcgt cctcgccgcg
      841 ctgcgcgacg ccacggccga ggggcgcggg gtgtggatcg gctacaccga ccagaacggc
      901 acagcgtcgc cggtcttctt ctaccccagc aggatcgagg cgggccgggc ctggggggcg
      961 gtacccgctg cccgtgagga ccgtgggttc ctgttgcacc ggatcaccgg ggtcgtcgcg
     1021 tcctaggctg cgccgccggc gagagcgcgc acgacgcgtg acggggaggg ccggccgagc
     1081 tggcgggcga gccacgcact cgtctccacg agggcctcga ggtcgacacc ggtctcgact
     1141 ccggccccgt gcagggccca gaccaggtct tcggtagcca ggttgcctgt cgcgctcttg
     1201 gcgtagggac acccgccgag cccgccggcc gacgcgtcga cgacggtgac cccgtcagcc
     1261 agggcggcca tcgtgttggc cagggcctgg ccgtaggtgt cgtggaagtg cacggcaacc
     1321 cggtcgaggc cgatccccgc ggcggcgagc gccgcgagga gccggtggac atgcccggtc
     1381 gtcccgacgc cgatcgtgtc gccgatgctc agctcgtcgc agccgaggtc catgagccgc
     1441 cgggcgacct cgacgacctg gccgaccggc acgtcgccct cccacgggtc cccgaagcac
     1501 atcgacacgt aggcccgcac ccacagccct tcggcgcggg cccgggccac gaccggctcg
     1561 aacatcgtga cggactcggc gacgctgcgg ttgaggttct tgcgggc
//
LOCUS       DATAIX010000263         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_44111, whole genome shotgun sequence.
ACCESSION   DATAIX010000263 DATAIX010000000
VERSION     DATAIX010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44111"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VHM65_03920"
     CDS             complement(<1..637)
                     /locus_tag="VHM65_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704881.1"
                     /translation="MTAVLTAVGLRWESQVATVLERSREVTIARRCADLADLLATAAA
                     GLGSVALVSDDLRGLDLSTVEYLRSCGLRVVGLTCTGEEAAERLLRQIGVLVVLPVSS
                     GLTALEAALLGDHGESGVITAEWTAGRVAPLPDGAGAHAVKGVPRPQPGLPDPAEPDL
                     GPDRSRGRVIAVWGPTGAPGRTMVAVNVAAEVAQAGISTLLVDADTYGGCVA"
     gene            complement(634..1278)
                     /locus_tag="VHM65_03925"
     CDS             complement(634..1278)
                     /locus_tag="VHM65_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704882.1"
                     /translation="MAKRLQRPSWRDSRLVVGLLLVLTATTIGAKVVSGADDRIPMYA
                     ARVALQPGDRLSADNLARVDVQLGDGVAAYLKVEGPLPADRYATRQVGAGELVPVAAT
                     GSAQDVTVQRVTVQVDALSAAALVVGSVVDVYVNERDPQATQEKYLDPKKTLESVTVT
                     WLPQDTTRFGAGNATSAIQIAAPIGQVQRLIAAIDRKARVTVVPVAGSDLKKGP"
BASE COUNT          263 a          577 c          619 g          231 t
ORIGIN      
        1 gtgcgacgca cccgccatag gtgtcagcgt ccacgagcag ggtggagatg cccgcctgcg
       61 ccacctcggc cgcgacgttg accgcgacca tcgttcgacc cggggcgccc gtaggccccc
      121 agacggcgat gacgcgcccg cgcgagcggt cggggccgag gtcgggctcc gcggggtcgg
      181 gcaggcctgg ctgcggccga ggtacaccct tgaccgcgtg cgcaccggcg ccgtcgggca
      241 ggggcgccac gcggccggcg gtccactccg ccgtgatgac cccggactcc ccatggtcac
      301 cgagcagagc cgcctcgagt gccgtcaggc ccgaggacac gggaaggacg acgaggaccc
      361 cgatctggcg cagcaggcgc tcggccgcct cctcgccggt gcaggtcagt cccaccacgc
      421 gcaggccgca cgagcgcagg tactccaccg tgctgaggtc gagcccgcgc aggtcgtccg
      481 agacgagcgc caccgacccg agccccgcgg cagccgtcgc gagcagatcc gcgaggtcgg
      541 cgcaccggcg cgcgatcgtc acctcacggg agcgctcgag gaccgtggcg acctggctct
      601 cccagcgcag gccgaccgcc gtgaggactg cggtcatggt cccttcttga ggtcggagcc
      661 ggcgacgggg accaccgtga cccgggcctt gcggtcgatc gcggcgatga gccgctgcac
      721 ctggccgatc ggggcagcga tctggatcgc cgaggtcgcg ttgcccgccc cgaaccgggt
      781 cgtgtcctgg ggtagccagg tgaccgtgac cgactcgagg gtcttcttgg ggtcgaggta
      841 cttctcctgc gtcgcctgcg ggtcgcgctc gttgacgtag acgtcgacaa ccgagccgac
      901 gaccagagca gcggccgaga gcgcgtcgac ctgcacggtg acccgctgga cggtcacgtc
      961 ctgtgcgctg cccgtggccg cgacggggac gagctcaccc gcaccgacct ggcgggtggc
     1021 atagcggtct gcgggcagcg gcccctcgac cttgaggtat gccgcgacgc cgtcaccgag
     1081 ctggacgtcg acgcgggcga ggttgtccgc gctgagcctg tcccctggct gcaaggccac
     1141 tcgcgcggcg tacatgggga tgcggtcgtc ggcgcccgag acgaccttgg ccccgatcgt
     1201 cgtcgcggtg aggacgagca ggagaccgac gaccaggcgc gagtcgcgcc aggacggccg
     1261 ttggaggcgc ttcgccacgg gtgtggggag ctgcggcacc cgatccccct gaacggttgt
     1321 ggtgcggtga ggcacgcggt gacggtgggc gggcggaggg tcatgccacc gatcggggct
     1381 caccaagggc gaacccatca tggccgacga aaccatccgg tgtcacaatc atccccaatc
     1441 cagcggacgt gcgagaaccg gggacacgcg gcctgtggat aacggtcacc agaggggcgc
     1501 gagtcgggta cagtaaaggg atagtaaaga actcacacac gtgttcgaaa gcaggtcgat
     1561 ttctatggag cagcgcttcc ttcagctgac cgaggtatcc gaggtcctca acatctcctc
     1621 ggctcaggcg tacgccttgg tacgctccgg ggagctaccg gccatcaagg tggggggccg
     1681 tggccagtgg
//
LOCUS       DATAIX010000264         1812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_103608, whole genome shotgun sequence.
ACCESSION   DATAIX010000264 DATAIX010000000
VERSION     DATAIX010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103608"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..865
                     /locus_tag="VHM65_03930"
     CDS             245..865
                     /locus_tag="VHM65_03930"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX2704883.1"
                     /translation="MVNLTRIYTRTGDDGTTALGDFSRTHKTDPRLAAYADANEANAA
                     IGVAVACGRLPADVVEVLHRVQNELFDIGADLCTPLAETYQYPPLRVQQEWVDDLESD
                     CDRFLEQTEPLRSFILPGGTPGAAHLHVATTVVRRAERRTWAALERYGAQPGTERGAG
                     GINPLTATYLNRLSDLLFILARVANHPIGGDVLWQPGGGRVAPPQR"
     gene            complement(903..1340)
                     /locus_tag="VHM65_03935"
     CDS             complement(903..1340)
                     /locus_tag="VHM65_03935"
                     /inference="COORDINATES: protein motif:HMM:NF022199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2550 family protein"
                     /protein_id="HEX2704884.1"
                     /translation="MPSALVSAEVVIGCALVLVALFLTWTFVRRSMICRGRTVLVCGW
                     RGADASVWQAGLLGLEGEQLEWFPLSGVRIQPRFCWDRTALEVDAPRPVPSTTGLGID
                     GSAFALTCRYGGALFDIALDDPSYTALRSWLDSVPPGYNVNVA"
     gene            complement(1345..1611)
                     /locus_tag="VHM65_03940"
     CDS             complement(1345..1611)
                     /locus_tag="VHM65_03940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009977.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX2704885.1"
                     /translation="MSELQVEMVAADRLVWSGTASMVRARSVDGDLGILPGHAPLLAI
                     LSSGDVSVKAVSGPDVSATIDSGFLSVEHDRVTIVAEHVTTSTG"
BASE COUNT          322 a          643 c          599 g          248 t
ORIGIN      
        1 cgcgatgcgc catcctgact ccttcgtcga ggacctgctc acgtcaacga aacgccatgc
       61 gggccgaagg gctgccacag ggcccggctc caaccatgta gaccccttgc ggcgccaacg
      121 accgcgacca agcagaacct aacgcccgcc atgtgcacct gcgcggacga cacgctgtga
      181 gatatgtgac agaagtgctc ggcgtgcatg ttgtgatcac cgcgtgatct gcgaggcttg
      241 ggccatggtc aacctcacgc gcatctacac tcgcaccggt gacgacggca caacggccct
      301 cggggacttc agccggaccc acaagaccga cccacgcctg gccgcctacg ccgacgcgaa
      361 cgaggccaat gccgccatcg gggtcgccgt agcgtgcggc cggctccccg cggacgtcgt
      421 cgaggtcctg catcgggtcc agaacgagct cttcgacatc ggcgcggacc tgtgcactcc
      481 cctcgccgag acctaccagt accccccgct gcgagtgcag caggagtggg tcgacgacct
      541 tgagtccgac tgcgaccgct tcctcgagca gaccgagccc ctgcgctcgt tcatcctccc
      601 cggcggcacc ccgggggcgg cccacctgca cgttgccacg accgtcgtga ggcgcgcgga
      661 acggcgcacc tgggcagcac tggagaggta tggcgcgcag cctggcaccg agcggggtgc
      721 gggcgggatc aacccactca cggcgaccta tctcaaccgg ctctcggacc tgctgttcat
      781 cctggcccgc gtggccaacc acccgatcgg cggcgacgtc ctgtggcagc ccggcggtgg
      841 ccgggtcgcg ccgccgcagc gttgatcgtc aggccatgtt gatcgtcagg ccacgctgag
      901 cgtcaggcca cgttgacgtt gtaccccggc gggaccgagt cgagccagga ccgaagggcg
      961 gtgtagctcg ggtcgtcgag ggcgatgtcg aagagggcac cgccgtatcg acaggtcagc
     1021 gcgaacgcgg aaccgtcgat gcccagaccc gtcgtcgagg ggaccggccg cggcgcgtcg
     1081 acctccaacg cggtccggtc ccagcagaag cgcggctgaa tcctcacccc gctcaggggg
     1141 aaccattcca gctgttcgcc ctcgaggccg agcagccccg cctgccagac tgacgcgtca
     1201 gcgccgcgcc agccgcagac cagcacggtg cggccacggc agatcatgga acgccggaca
     1261 aaggtccagg tgaggaagag cgccacgagc accaacgcgc agccgatgac cacctcggcg
     1321 gacaccagcg cggatggcac gaggtcagcc cgtggaggtc gtgacgtgct ccgccacgat
     1381 ggtcactcgg tcgtgctcga cagaaaggaa cccgctgtcg atcgtcgccg agacgtctgg
     1441 tcccgacacc gccttgaccg acacgtcgcc cgagctgagg atcgcgagca agggagcgtg
     1501 accaggcagg atgcccaggt cgccgtcgac cgaccgagcg cgaaccatcg acgccgtgcc
     1561 ggaccagacc aggcggtcgg cggcaaccat ctcgacctga agctcactca cgatgtcctc
     1621 cgctgctgat agtccggaca gtctagcggg cacggctcgg ccctttgggc cgggctggcc
     1681 tgagtgtggc tcacgccacc agctcgtcga gcgcagccgc aaagacctcg tggtgacggg
     1741 caacgtcggc gctggtcgtg accgggcaca tgagggccat gttgtggaac ggggtgagca
     1801 gcactccccg gt
//
LOCUS       DATAIX010000265         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_185042, whole genome shotgun sequence.
ACCESSION   DATAIX010000265 DATAIX010000000
VERSION     DATAIX010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185042"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="VHM65_03945"
     CDS             <1..1276
                     /locus_tag="VHM65_03945"
                     /inference="COORDINATES: protein motif:HMM:NF044853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEX2704886.1"
                     /translation="IYITPIHPIGSAAKKGRNNSLNARPDDPGSPYAIGSPDGGHDAI
                     HPDLGTFDDFDAFVARAAGLGLEVAMDLALQCSPDHPWVVSHPEWFTTRADGTIAFAE
                     NPPKKYQDIYPMNFDNDPAGVYAEVRRVIQVWIDHGVRIFRVDNPHTKPVEFWQWVIR
                     DVAERHPEVIWLAEAFTQPAMMHTLGKAGFQQSYTYFTWRNLKWELEEYARELSGDAA
                     SYMRPSFWPTTHDILTPYMQYGGPTAWKLRAALAGTLVPTYGIYAGYELMECVARPGA
                     EEQIDNEKYQYRDRRWSEYEPGGAKFKRSLAPYLTRINEIRRAHPAMHWLRNLAFHHV
                     EDENLMCFSKSRWVGGADGYRDTVIVVANLDPHATRESQVHLDMAALHMSWHDQFVVQ
                     DLITDATWSWAEHNYVRIGPETEPVHILAVRS"
     gene            1273..>1574
                     /locus_tag="VHM65_03950"
     CDS             1273..>1574
                     /locus_tag="VHM65_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX2704887.1"
                     /translation="MSLPGLNVGQPGLRHDPDWFKTAVFYEVLVRAFADSKGSGAGDI
                     TGLIAKLDYLQWLGVDCLWLPPFYASPLRDGGYDIADYRSVLAEFGTLPEFTELVS"
BASE COUNT          273 a          571 c          494 g          236 t
ORIGIN      
        1 catctacatc acccccatcc acccgatcgg cagcgccgcc aagaagggcc gcaacaacag
       61 cctcaacgcc cgcccggacg accccggcag cccgtacgcg atcggctcgc ccgacggcgg
      121 gcatgacgcg atccaccccg acctcgggac cttcgacgac ttcgacgcgt tcgtcgcgcg
      181 cgccgccggg ctgggcctgg aggtcgccat ggacctcgcc ctgcagtgct caccagacca
      241 cccctgggtg gtgtcccacc ccgagtggtt cacgacccgg gccgacggca cgatcgcctt
      301 cgccgagaac ccgccgaaga agtaccagga catctacccg atgaacttcg acaacgaccc
      361 cgcgggggtc tacgcggagg tgcgccgggt catccaggtg tggatcgacc acggtgtcag
      421 gatcttccgg gtcgacaacc cgcacaccaa gccggtcgag ttctggcagt gggtcatcag
      481 ggatgttgcc gagcggcatc ccgaggtcat ctggctcgcc gaggccttca cccagcccgc
      541 gatgatgcac acgctcggca aggccggctt ccagcagagc tacacctact tcacctggcg
      601 caatctcaag tgggagctcg aggagtacgc ccgcgagctg tccggcgacg ccgcgtcata
      661 catgcgcccg tcgttctggc ccacgacgca tgacatcctc acgccctaca tgcagtacgg
      721 cggacccacc gcgtggaagc tgcgggcggc gctggcgggg acgctcgtgc cgacctacgg
      781 catctacgcc gggtacgagc tgatggagtg cgtggcgcgt cccggcgcgg aggagcagat
      841 cgacaacgag aagtaccagt acagggaccg gcgctggtcc gagtacgagc cgggcggagc
      901 caagttcaag cggtcgctcg cgccatacct gaccaggatc aacgagatcc gccgtgccca
      961 ccctgccatg cactggctgc gcaacctggc gttccatcac gtcgaggacg agaacctcat
     1021 gtgcttctcg aagtcacgct gggtgggcgg cgccgatggc tatcgcgaca cggtcatcgt
     1081 cgtcgcgaac ctcgacccgc acgcgacccg ggagtcgcag gtccacctcg acatggcggc
     1141 gctgcacatg agctggcacg accagttcgt cgtccaggac ctcatcaccg acgcgacgtg
     1201 gtcctgggcc gagcacaact acgtgcgtat cggcccagag acggagccgg tgcacatcct
     1261 ggccgtgcgg tcatgagcct gcccggcctc aacgtcggcc agccgggact gcggcacgac
     1321 ccggactggt tcaagacggc cgtgttctac gaggtcctcg tgcgggcctt cgccgactcc
     1381 aagggctcgg gcgcaggcga catcaccggc ctcatcgcca agctcgacta cctgcagtgg
     1441 ctcggcgtcg actgcctctg gctgccgccg ttctacgcct ctcccctgcg cgacggcggc
     1501 tacgacatcg ccgactaccg ctcggtgctc gcggagttcg ggacgctgcc cgagttcacc
     1561 gagctcgtct cgca
//
LOCUS       DATAIX010000266         2941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_14087, whole genome shotgun sequence.
ACCESSION   DATAIX010000266 DATAIX010000000
VERSION     DATAIX010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14087"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1415
                     /locus_tag="VHM65_03955"
     CDS             180..1415
                     /locus_tag="VHM65_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704888.1"
                     /translation="MRTYPYAFHIALDGNGLNGLEGRAGVCLFHYDPGTGDYAYKVTY
                     FGGVTGGHAPNVDPANRIGFLGNSGQHLLFYDRSSLDEVERISTLRFEIPDTTVKGST
                     HLVWLSDTEFLTSIGEHLWRFDINRLAKAEIVAPHQLRLPHAMKRTASGRYVVYGGMD
                     HPRDGEAREVGVLDTHTGAVRRVPLPTTCWHLVCHPVLDVCYAMSFRVGPSDGTDWAQ
                     WGMAYQRQYAFEIDLEECLVTRHWTGEPALPVHVNSDVTISDTELIFCTGGSHSVVLV
                     DLATLSRVRIIDEHPGALQSLAWSRESMTTLSQVVSRANPFTNAHLYASSLMVSRGAL
                     IDGIYACQLSADRSLLFTANRGQNHITVYDYPTLERRLRVPMPELQEFDVGLGEWADP
                     RLGFHHSHLTSPAGRTLDG"
     gene            1408..1863
                     /locus_tag="VHM65_03960"
     CDS             1408..1863
                     /locus_tag="VHM65_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704889.1"
                     /translation="MADYSFDAAVHGTLGHNVTSELVGLDDMSLTLAGGTTTTLAGEN
                     TVHSDATVEVKPLTTTSDITLEPVTTTSDVRLELAPIQTHSAIDLEPVAVDSCLRIEL
                     GPLPATDVHSPYEQRWSWAVLGTELFSLSVCGHSSTHLAPGGRRPHVID"
     gene            1880..2596
                     /locus_tag="VHM65_03965"
     CDS             1880..2596
                     /locus_tag="VHM65_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704890.1"
                     /translation="MARRPPLTAERPLVLSGPPQRVQGTFTVENTGSSRLGGRGAVLM
                     RDGHPGVTGQASALIPPGATEQVDLTIPMDPLTEPGEYAAQLDVGGVLRPVVVVVEPA
                     LAMRVSPSKVLAGPGRTSLELLVRNDGNVAIPLARTTRSWTLPSGTGDADHGPEVTLR
                     LGTDGLVLEPGDVVRVMATLEVPEDLDREHRHESRLPLGLADLTVLVLPRDGAPVSTP
                     TTRSTGRARRPRTTSTKETP"
     gene            2593..>2941
                     /locus_tag="VHM65_03970"
     CDS             2593..>2941
                     /locus_tag="VHM65_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704891.1"
                     /translation="MSTTTPDLMDRWARTMDQASQLAWDWWDQSQAMMEPWRDVVTGM
                     ATSPEPDDHDHDHGRHHDHDHRGGRDGCGCHDCDRHPGRQRGRGGQQPCGCEKRDARA
                     GSAQHHGSSCRRAD"
BASE COUNT          465 a         1132 c          947 g          397 t
ORIGIN      
        1 gctccccacc gtcatccggc acggcgccct ggtcaacacc cggggcggtg gacgtcggcg
       61 ccgcccgccg gcccggggga ggaagtcgtg agcgcccctg tccctgagca gagcccgccg
      121 gtacagtcgg gctacgaccg acgcacccgc gcggccgtct cgccaggcac gaggccacag
      181 tgcggacgta cccctacgcc ttccacatcg ccctcgacgg caacgggctc aacgggctcg
      241 agggccgggc gggcgtgtgc ctcttccact acgaccccgg caccggggac tacgcctaca
      301 aggtgaccta cttcggcggg gtcaccggcg ggcacgcgcc gaacgtcgac ccggccaacc
      361 ggatcggctt cctcggcaac tccggccagc acctgctctt ctacgaccgg tcctccctcg
      421 acgaggtcga gcgcatctcg acgttgcgct tcgagatccc ggacacgacg gtcaagggga
      481 gcacgcacct cgtctggctc agcgacaccg agttcctcac ctcgatcggg gagcacctgt
      541 ggcgcttcga catcaaccgt ctcgccaagg ccgagatcgt cgccccgcac cagctgcggc
      601 tcccgcacgc gatgaagcgg acggcatcgg gacgctacgt cgtgtatggc gggatggacc
      661 acccccgcga cggtgaggct cgggaggtgg gcgtcctcga cacccacacc ggcgcggtcc
      721 gccgggtgcc gctcccgacg acctgctggc acctcgtgtg ccatcccgtg ctcgacgtgt
      781 gctatgccat gtcgttccgg gtcggaccct cggacggcac cgactgggcg cagtggggga
      841 tggcctacca gcggcagtac gccttcgaga tcgacctgga ggagtgcctc gtcacccggc
      901 actggaccgg tgagcccgcc ctgccggtcc acgtcaactc cgatgtgacg atctcggaca
      961 ccgagctcat cttctgcacc ggtggcagcc actccgtcgt cctcgtcgac ctggccaccc
     1021 tgagccgcgt ccgcatcatc gacgagcatc cgggggcact ccagtccctg gcgtggtccc
     1081 gggagtcgat gacgaccttg agccaggtgg tgagccgggc caaccccttc accaacgctc
     1141 atctctacgc ctcctcgctc atggtgagcc gtggggcgct catcgacggc atctacgcct
     1201 gccagctctc ggcggaccgc tcgctgctct tcaccgccaa ccgcggccag aaccacatca
     1261 ccgtctatga ctaccccacc ctggaacgcc ggctgcgggt ccccatgccc gagctgcagg
     1321 agttcgacgt cggcctcggc gaatgggccg acccgcgcct cggcttccac cattcacacc
     1381 tgaccagccc cgccggaagg accctcgatg gctgactact cgttcgacgc ggccgtgcac
     1441 ggcactctcg gtcacaacgt cacgagcgag ctcgtcgggc ttgacgacat gtcgctgaca
     1501 ctcgcgggcg gcacgacgac aaccctggcc ggggagaaca ccgtccactc cgacgcgacc
     1561 gtcgaggtca agccgctcac gacgacgtcg gacatcaccc tcgaaccggt gacgacgacc
     1621 tccgacgtac gcctcgagct cgcgccgatc cagacccaca gcgcgatcga cctcgaaccc
     1681 gtcgccgtcg acagctgcct gcggatcgag ctcgggcccc tcccggcgac cgacgtccac
     1741 agcccctacg agcagcggtg gtcctgggcg gtgctcggga cggagctgtt ctcgttgagc
     1801 gtctgcgggc acagctcgac ccacctggct cccgggggcc gacgaccgca cgtgatcgac
     1861 tgagcgggag gatgaggcca tggccagacg accgcccctg acggccgagc gcccgctcgt
     1921 cctgagcggt ccgccacaac gggtccaggg caccttcacc gtcgagaaca ccggctcgag
     1981 ccggctcggt ggccgcggcg ccgtgctcat gcgcgacggc cacccgggag tcaccggcca
     2041 ggcctcggca ctcattcctc ccggggccac cgagcaggtc gacctcacca tccccatgga
     2101 cccactcacc gagcccggcg agtatgccgc ccagctcgac gtcggcgggg tgctccggcc
     2161 ggtcgtcgtc gtcgtcgaac ccgcgctggc gatgcgggtc tcgccatcga aggtgctggc
     2221 cggccccggc cggacgagcc tcgagctcct cgtccgcaac gacggcaacg tcgccatccc
     2281 cctcgcgcgg acgacccgca gctggaccct cccgagcggc acgggtgatg ccgaccatgg
     2341 cccggaggtg accctgcgcc tgggcaccga cggcctggtc ctcgagcccg gggacgtggt
     2401 ccgcgtcatg gccacactgg aggtaccaga ggacctcgac cgcgagcatc gccacgagag
     2461 ccggctgccc ctggggctcg ccgatctcac cgtgctcgtc ctcccgcgtg acggcgcacc
     2521 agtgagtaca ccgacgaccc gctcgaccgg gcgcgcccgg cgcccgcgaa ccacatcgac
     2581 gaaggagaca ccatgagcac cacaaccccc gacctcatgg accgctgggc caggacgatg
     2641 gatcaggcga gccagctcgc ctgggactgg tgggaccagt cccaggcgat gatggagccg
     2701 tggcgcgacg tcgtcacggg gatggcgacc agccccgagc ccgacgacca cgaccacgac
     2761 cacggccgcc atcacgacca cgaccaccgc ggcggtcgcg acggctgtgg gtgtcacgac
     2821 tgcgaccggc acccaggacg tcagcgcggc cgtggcgggc agcagccctg cgggtgcgag
     2881 aagcgggacg cccgagcggg ttcggcgcag caccacggtt cttcgtgccg ccgggccgac
     2941 g
//
LOCUS       DATAIX010000267         1698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_14103, whole genome shotgun sequence.
ACCESSION   DATAIX010000267 DATAIX010000000
VERSION     DATAIX010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14103"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VHM65_03975"
     CDS             complement(<1..273)
                     /locus_tag="VHM65_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX2704892.1"
                     /translation="MVAALSPSRASDFMTCPLLYRFRVIDRIPAPPSPAAARGTLVHA
                     VLERLFDLPCDERTLEAAVALVEPQWVRLVQEEPELAVLGSTDVEGA"
     gene            359..1498
                     /locus_tag="VHM65_03980"
     CDS             359..1498
                     /locus_tag="VHM65_03980"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX2704893.1"
                     /translation="MSAPRSYGWRIATVSGIPVYLGRSWPLIAVIIVAMFGPLIEHPT
                     DPVASPAYGYLVAVGYALVLLLSVLAHEGAHAAVARAVGCRVDRVVADLWGGHTVYES
                     GVTRPGTSATIAVSGPLANAALAALGYGAAQVVSDPTARVIIGALTVSNLFVAVFNML
                     PGLPLDGGYLVESAVWRITGDRNRGMVFAGHLGRLVTVAVVAWLVVDIARSGSTPSIV
                     TMIWILLIAGFLWRGATNAIAVGRTRLRLETIRVRDVLHPVVTLTLDASAAAVAGALL
                     TVAAANPLVVAVDAAGRPVGAVDPRAYAAIPQERRPVVPVSSLILAPAPGWVVTSDPD
                     DTVTDIVVSIQSSRADVVLVQDRAGRLLGYVTVDEIATRMSSARV"
BASE COUNT          215 a          620 c          615 g          248 t
ORIGIN      
        1 cgccccctcg acgtcggtgg acccgagcac ggccagctcg ggctcctcct gcacgagccg
       61 gacccactgc ggctcgacga gggcgaccgc cgcctcgagc gtgcgctcgt cgcagggcag
      121 gtcgaagagc cgctccagca cggcgtggac caacgtcccg cgcgctgctg ccgggctcgg
      181 tggggccggg atgcggtcga tgacccggaa gcggtacagc aacgggcacg tcatgaagtc
      241 cgacgcacgg ctcggggaga gggcggccac catggtctcg accctacggg gcagcggtga
      301 cagcccggca taccggtgac cggcctggtc cggcctctcc ctcacgtagg gtcacctcat
      361 gagcgcaccg aggtcctacg ggtggcggat cgccacggtg agtggcatcc cggtctacct
      421 cggcaggtcc tggccgctca tcgccgtcat catcgtggcc atgttcggcc ctctcatcga
      481 gcacccgacc gacccggtcg cgagcccggc ctacggctac ctcgtcgcag tcgggtacgc
      541 cctcgtcctg ctcctgtcgg tgctcgcgca cgagggcgcc cacgccgcgg tggcgcgagc
      601 cgtcgggtgc cgggtcgacc gagtggtcgc cgacctgtgg ggtgggcaca cggtctacga
      661 gtcgggagtg acgcgtccgg ggacgagcgc gaccattgcc gtgagcggac cgctcgcgaa
      721 cgccgctctg gcggccctcg ggtatggcgc cgcgcaggtc gtgagcgacc ccacggcgcg
      781 ggtgatcatc ggcgctctga cggtgtcgaa cctgttcgtc gccgtgttca acatgctccc
      841 cgggctgccg ctcgacggcg gctacctcgt cgagtcggcg gtgtggagga tcaccggtga
      901 ccgcaaccga gggatggtct tcgccgggca cctcggccgg ctcgtcacgg tcgccgtcgt
      961 ggcctggctc gtcgtcgaca tcgcgcgcag cgggtcgacg ccgtcgatcg tcacgatgat
     1021 ctggatcctg ctcatcgcgg ggttcctgtg gaggggcgcg acgaacgcca tcgcggtcgg
     1081 ccggacccgc ctcaggctcg agacgatccg ggtccgcgac gtgctgcacc cggtcgtcac
     1141 gctcaccctc gacgcaagcg ctgcggccgt ggctggcgcg ctgctgaccg tggccgcggc
     1201 caaccccctt gtcgtcgcgg tcgacgccgc cggccggccg gtcggggccg tcgacccacg
     1261 cgcctatgcc gccatcccgc aggagcgcag gcccgtcgtg ccggtgtcct cgctgatcct
     1321 ggccccggcc cctggatggg tcgtcacctc cgacccggac gacaccgtca ccgacatcgt
     1381 ggtgtcgatc cagtcgagcc gggccgacgt cgtgctcgtg caagaccgcg cggggcgact
     1441 cctgggctac gtcaccgtcg acgagatcgc gacccggatg tcgagcgccc gcgtctgacg
     1501 gcgcgtccgg cagcctctgg tgctgggggg cgcggcgggg ctggccgacg tgggtccgat
     1561 ccggccgacc cgcaccggcc acatccacct cagtagccag ggccggctga tcgaggccga
     1621 tccggccggc cagcaccggg cagggctggc agggggcagg gcttggccgg gagggccggc
     1681 acctagactg tgccacca
//
LOCUS       DATAIX010000268         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_125383, whole genome shotgun sequence.
ACCESSION   DATAIX010000268 DATAIX010000000
VERSION     DATAIX010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125383"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VHM65_03985"
     CDS             <1..688
                     /locus_tag="VHM65_03985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEX2704894.1"
                     /translation="PITLAHAVRSLLLDTAGLARQRWVQRGFRRPAGSTASMRTLMGQ
                     IDGTVNPRPGTPEFDDLVWVGDEGPSWLRGGTGLVVRRIRMNLAAWDLLDPPAKEEVI
                     GRRLSTGAPLTGTRETDPADLGATSATGLSAIPDFAHIRHAAAVLPRERFLRRPYNYD
                     ERATGAGEDSGLVFMTFQRDITEQFVPVQRRLADKDLLNMWTTPVGSAVFAILPGAAE
                     GEYLGERLYG"
     gene            830..>2008
                     /locus_tag="VHM65_03990"
     CDS             830..>2008
                     /locus_tag="VHM65_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX2704895.1"
                     /translation="MTQLVYAFAEGNKDLKDLLGGKGANLAEMTRMGLPVPPGFIITT
                     EACNAYLANGFVPDELAAQVSAHLTALEGQMGKRLGDAADPLLVSVRSGAKFSMPGMM
                     ETVLNIGLNDASVVGLAAQSGNDRFAQDSYRRLIQMFGKTVLGLHGDLFEHALEAAKA
                     DKGVAQDTDLGAEDLRALVATFKAIVERESGEAFPQEPRAQLNMAVEAVFRSWNAERA
                     ILYRRQERIPTDLGTAVNVCTMVFGNVGEGSGTGVAFTRDPASGHPGVYGDYLENAQG
                     EDVVAGIRNTIPLAALEGIDKSSYDELMAIMARLESHYQDLCDIEFTIERGKLWMLQT
                     RVGKRTARAAFRIATQLVDEGLIDESEALQRVTGDQLMQLMFPKFDESVPKQLIARGM
                     N"
BASE COUNT          326 a          733 c          658 g          291 t
ORIGIN      
        1 ccccatcacc ctcgcccatg cggtgcggtc gctcctgctc gatacggcgg gcctggcccg
       61 ccagcgctgg gtacagcgcg gcttccgccg gccggcgggg tcgaccgcct cgatgcgcac
      121 cctcatgggc cagatcgacg ggaccgtgaa ccccaggccg gggacgcccg agttcgacga
      181 cctcgtctgg gtcggcgacg aaggaccgtc ctggctgcgc ggtggcaccg gcctggtcgt
      241 gcgccggatc cggatgaacc tggccgcgtg ggacctgctc gacccgccgg ccaaggagga
      301 ggtcatcggg cggcgcctgt ccaccggggc tccgctgacc ggcacgcggg agacggaccc
      361 ggctgacctc ggggcgacgt cggcgaccgg gctgtccgcc ataccggact tcgcccatat
      421 ccggcacgcc gctgcggtcc tgccccggga gcgtttcctg cggcggccgt acaactacga
      481 cgagagggcg accggagccg gcgaggactc gggactggtg ttcatgacct tccagcgcga
      541 catcaccgag cagttcgtcc cggttcagcg ccggctcgcg gacaaggacc tgctcaacat
      601 gtggacgacg cccgtaggat cggccgtgtt cgccatcctc ccgggcgccg ccgagggtga
      661 gtatcttggc gagagactct atggctgacg accggttttc gttgcccgtc ggccggatga
      721 gagagaatgg acacgtccga aggggcccta ggtcccgaac ccccgctcgc gccgccccta
      781 cgttcgcggt tgactacagc cagtttccca cccgctgaaa ggccatgcca tgacccagct
      841 cgtctacgcg ttcgctgaag gcaacaagga cctcaaggac ctcctcggag gcaagggggc
      901 caaccttgcc gagatgacgc gcatggggct acccgtacca ccgggtttca tcatcaccac
      961 cgaggcctgc aacgcctacc tcgccaacgg cttcgtcccc gacgagctcg ccgcgcaggt
     1021 cagcgcccac ctcaccgccc tcgaagggca gatgggcaag cgcctcggcg acgccgccga
     1081 cccgctgctc gtgtccgtcc gctcgggcgc gaagttctcg atgcccggga tgatggagac
     1141 cgtcctcaac atcggcctca acgacgcctc ggtcgtgggg ctcgccgccc agtcgggcaa
     1201 cgaccgcttc gcccaggact cctaccgccg cctgatccag atgttcggca agaccgtgct
     1261 cggcctgcac ggcgacctgt tcgagcacgc cctggaagcc gccaaggccg acaagggcgt
     1321 cgcccaggac accgacctcg gcgccgagga cctgcgggcc ctcgtggcca cgttcaaggc
     1381 gatcgtcgag cgcgagtcgg gcgaggcatt cccgcaggag ccccgggccc agctcaacat
     1441 ggccgtcgag gccgtcttcc gctcgtggaa cgccgagcgg gcgatcctct accgccgtca
     1501 ggagcgcatc cccaccgacc tcggcaccgc cgtcaacgtg tgcacgatgg tcttcggcaa
     1561 cgtcggcgag ggatcgggga ccggcgtcgc cttcacccgt gaccccgcct ccggccaccc
     1621 cggcgtgtat ggcgactacc tcgagaacgc gcagggcgag gacgtcgtcg ccgggatccg
     1681 caacacgatc ccgctcgcgg cgctcgaagg gatcgacaag tcgtcctacg acgagctcat
     1741 ggcgatcatg gcccggctcg agtcgcacta ccaggacctg tgtgacatcg agttcaccat
     1801 cgagcgcggc aagctgtgga tgctgcagac ccgcgtcggc aaacgcacgg cccgggccgc
     1861 cttccggatc gcgacccagc tcgtcgacga gggcctcatc gacgagagcg aggccctgca
     1921 gcgggtcacc ggtgaccagc tcatgcagct gatgttcccc aagttcgacg agtcggtgcc
     1981 gaagcagctc atcgccaggg gcatgaac
//
LOCUS       DATAIX010000269         4569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_74316, whole genome shotgun sequence.
ACCESSION   DATAIX010000269 DATAIX010000000
VERSION     DATAIX010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74316"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            398..1132
                     /locus_tag="VHM65_03995"
     CDS             398..1132
                     /locus_tag="VHM65_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEX2704896.1"
                     /translation="MIDYLGHLESAAASLAGALRRGDLEAPVLACPGWTLRDLGVHVA
                     GAHHWARGAILEGHPRTPPPEPPADTGPAALADWYLGHASELRHTLACTDPEAPCWTF
                     GPKPSTVAFWHRRQAHETTVHAWDADPASRVDLALAVDGIDEVVRMFFPRQVRLRRMA
                     PLAGSLALAPDDLVPSGTPARWVLTGDGLTPHPSPVGDVDATVSGPADVLYLLLWGRI
                     DVGDGRVRISGDADAARRILTSGIVP"
     gene            1205..2266
                     /locus_tag="VHM65_04000"
     CDS             1205..2266
                     /locus_tag="VHM65_04000"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF002898.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX2704897.1"
                     /translation="MSVSPTRSLNVAVIAGDGIGPEVVAQGLKVLGAAVGASVSVTAT
                     PYDLGARRWHATGETLPDSVLEEIRQHDVILLGALGDPSVPSGVLERGVLLPLRFALD
                     HYVNLRPAKLYPGVSSPLDVASVARHGIDFIVVREGTEGPYVGNGGALRVGTPHEIAT
                     EVSVNTAYGVERIVRAAFQKAEARDRQHLTLVHKHNVLTFAGHLWRRTVERVAADYPQ
                     VEMAYQHVDAATIFLITDPGRFDVIVTDNLFGDIITDIAAAIAGGIGLAASGNINPDR
                     TTPSMFEPVHGSAPDIAGQGKADPTATILSVAMLLEHVGLRDAGVRVEAAVAKDLADR
                     GPAVRSTAQVGDAIATLVG"
     gene            2406..3512
                     /locus_tag="VHM65_04005"
     CDS             2406..3512
                     /locus_tag="VHM65_04005"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEX2704898.1"
                     /translation="MTTSTMTFEVTRRPDPVPGDRRAEILAHPGFGKYFTDHMLRATW
                     RSGEGWGRGEVAAYRPFVLDPAAAVLHYAQEIFEGMKAYRHADGSIWTFRPEANAQRF
                     VRSATRLALPTLPEEDFVESLRALVEVDQAWVPEPAGGSETSLYLRPFMFASETFLGV
                     RPAAEVTYSVIASPAGAYFASGIKPVTLWVSSQYARAGAGGTGAAKCGGNYASSLAGQ
                     LEGSAHGCDQAVFLDSSTQTFIEELGGMNLFFVYQDGRIVTPALSGSILEGVTRSSVL
                     ELGKELGLDPEERQVPMEEWKEGARSGEIAEVFACGTAAVVTPVGELRWEGGSADHRR
                     PGHVDEVALKIRAALLDIQYGRAEDAHGWLTRLV"
     gene            3509..4342
                     /locus_tag="VHM65_04010"
     CDS             3509..4342
                     /locus_tag="VHM65_04010"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2704899.1"
                     /translation="MTSAADGGSAWPRLIATDLDGTLLRSDGTVSTRTRAALAAVQAR
                     GIPLVFVTARPPRWLDELAELVGSHGTVLCGNGAFVYDVRARAVISVHPLSFTDIGSI
                     AADLRRTLPGTRFAAERATGFAYEAQFASGHPVPAAAACGPIESHTDSPIGKLLARNP
                     ALPDEEFLARVVEIVGDRAIVAYSGAGGLAEMSAPGVTKAVALQRLTGSLGIGATGVW
                     AFGDMPNDLPMMRWAGRSIAVANAHPDVLAVASHTCAGNDEDGVAEVLERLVRDMDRP
                     G"
BASE COUNT          666 a         1690 c         1561 g          652 t
ORIGIN      
        1 actccagccc accggtcgac tccagcccac cggtcgactc cagcccaccg gtcgactcca
       61 gccgtctggt cgaccgagag gggaggccct gacagcagcg gacgcgccac gataggcctg
      121 gctggtgcgc gccgccaggg cagtgagccg cgtcatacgt ccgcatcctg ggacaggatg
      181 taccagatag cgggacgtcc ttctactctc gcaggagcta cgccactcgc ggcgaccctg
      241 cagtggcgcc gacccgctca ccgaccggtt cgagcccagg cccgccacgc tgtcccgagg
      301 acgtgaccca tgaacgaaga agccgagaac gcccgccagg cgatccagga gtcgatggac
      361 ctgcgtgacc agggccactc cgacgactga gcgccgcgtg atcgactacc tgggccacct
      421 cgagtcggcc gccgccagct tggccggcgc actgcgccgt ggcgacctgg aggcgcccgt
      481 gctggcctgc cccggctgga cgctgcgaga tctcggtgtg cacgttgccg gggcccatca
      541 ctgggcccgc ggggcgatcc tcgaggggca cccccgcacc ccaccacccg agccacctgc
      601 cgacaccggc ccggcggccc tggccgactg gtacctcggc cacgcgagcg agctgcgtca
      661 taccctggcg tgcaccgacc ccgaggcccc gtgctggacc ttcgggccga agccgtcgac
      721 agtggccttc tggcaccgcc gtcaggccca cgagacgacg gtccacgcct gggacgccga
      781 ccccgcgtct cgcgtcgacc tcgccctcgc cgtcgacggg atcgacgagg tcgtccggat
      841 gttcttcccg cgccaggtcc ggctccgccg gatggcgccc ctggccggct cgctggcgct
      901 cgctccggat gatctggtgc cctcgggcac ccctgctcgg tgggtgctca ccggggacgg
      961 gctgacaccg caccccagcc cggtcggcga cgtcgacgcg accgtctcgg gcccggccga
     1021 tgtgctctac ctgctgctgt ggggccggat cgacgtcggc gacggccggg tccgcatcag
     1081 cggcgacgcc gacgcggcga ggcgcatcct caccagcggc atcgtcccct gacccgagag
     1141 gtttcaaagc ccgagacgcc agtcccaacc tgcaagacgc ctggctactg tggagcacat
     1201 gccgatgtcc gtctcaccca cccgctcgct caacgtcgcc gtcattgccg gtgacgggat
     1261 cggccctgag gtggtcgcgc agggactcaa ggtcctcggg gctgccgtcg gcgcgagcgt
     1321 ctcggtgacg gccactccct acgacctcgg cgcgcggcgc tggcacgcga cgggggagac
     1381 cctcccggac tcggtgctcg aggagatccg ccagcacgat gtcatcctgc tcggcgccct
     1441 cggcgatccg agcgtcccca gcggcgtcct cgagcgtggc gtcctgctgc cgctgcgctt
     1501 cgccctcgac cactacgtca acctccgccc ggccaagctc taccccgggg tgtccagccc
     1561 gctcgacgtg gccagcgtcg cccgccacgg gatcgacttc atcgtcgtgc gcgagggcac
     1621 cgagggcccg tatgtcggca acggtggcgc gctgcgcgtg ggaaccccgc acgagatcgc
     1681 caccgaggtg agcgtcaaca cggcgtatgg cgtcgagcgg atcgtgcggg cggccttcca
     1741 gaaggccgag gcccgggacc gccagcacct cacgctcgtg cacaagcaca acgtcctcac
     1801 cttcgccggg cacctctggc ggcgaacggt cgagcgggtc gcggcggact acccgcaggt
     1861 cgagatggcc taccagcacg tcgacgcggc cacgatcttc ctcatcaccg acccgggccg
     1921 gtttgacgtc atcgtcaccg acaacctctt cggcgacatc atcaccgaca tcgccgccgc
     1981 catcgccggt ggcatcggcc tcgcggcgag cggcaacatc aacccggacc gcaccactcc
     2041 gagcatgttc gagcccgtcc acggctcggc gccggacatc gccggccagg gcaaggccga
     2101 cccgaccgcg acgatcctgt cggtcgcgat gctcctcgag cacgtcggcc tgcgtgacgc
     2161 aggcgttcgg gtcgaggctg cggtggccaa ggacctcgcc gacaggggcc cggcagtgcg
     2221 ctcgaccgca caggtcggcg acgcgatcgc cacgctcgtc ggttagccgc cgcgggacct
     2281 tcggcgcacg gccaggacgg gataacctgc tgggcagggc ccgaccgccc tgctcaccac
     2341 aacagacacc acacgactga caccacacga ctgacaccac acgacagaca ccaggagaac
     2401 gacccatgac gacgtcgacg atgacgttcg aagtgactcg ccggcccgac ccggtgccgg
     2461 gcgaccgccg cgccgagatc ctggcccacc ccgggttcgg caagtacttc accgaccaca
     2521 tgctccgcgc gacctggcgc tccggcgagg gctggggcag gggcgaggtc gcggcatacc
     2581 ggccgttcgt gctcgacccg gccgccgcgg tcctgcacta cgcgcaggag atcttcgagg
     2641 gcatgaaggc ctaccggcac gccgacgggt cgatctggac gttccgtccc gaggccaacg
     2701 cccagcgctt cgtccgcagt gcgacccggc tggccctgcc gacgctgccc gaggaggact
     2761 tcgtcgagtc gctgcgtgct ctcgtcgagg tcgaccaggc ctgggtgccc gagccggcag
     2821 gcggtagtga gacgagcctc tacctgcggc ccttcatgtt cgcctccgag accttcctcg
     2881 gtgtccggcc ggctgcggag gttacctact cggtcatcgc ctctcccgcg ggtgcctact
     2941 tcgccagtgg gatcaagccg gtcaccctgt gggtgtcctc gcagtacgcc cgcgcgggcg
     3001 ccggtgggac gggtgcggcc aagtgcggcg gcaactatgc ctccagcctg gccggacagc
     3061 tcgagggcag tgcccacggc tgcgaccagg ccgtcttcct cgactcctcg acccagacgt
     3121 tcatcgagga gctcggtggg atgaacctgt tcttcgtcta ccaggacggg cggatcgtca
     3181 cccctgcgct gagcgggtcc atcctcgaag gggtcacccg ctcctcggtg ctcgagctcg
     3241 gcaaggagct cggcttggac cccgaagagc gacaggtccc gatggaggag tggaaggagg
     3301 gcgcccggag cggcgagatc gccgaggtct tcgcgtgcgg gaccgcggcc gtcgtcactc
     3361 ccgtcggcga gctgcgctgg gagggcggca gcgccgacca ccgccggcca ggccacgtcg
     3421 acgaggtggc gctgaagatc cgcgccgccc tgctcgacat ccagtacggc cgtgccgagg
     3481 acgcgcacgg gtggctgact cgcctcgtgt gacatccgcg gcggacgggg gttcggcctg
     3541 gccgcgcctc atcgccaccg acctcgacgg cactctgctg cgcagtgacg ggaccgtctc
     3601 gacccgcacc cgcgcggcgc tggccgcggt ccaggcgcgc ggcatacccc tggtgttcgt
     3661 caccgcgcga ccgccgcggt ggctcgacga gctcgccgag ctcgtgggtt cgcatggcac
     3721 cgtcctgtgt ggcaacggcg ccttcgtgta tgacgtgcgc gcccgggccg tcatctccgt
     3781 gcacccgctc tcgttcacgg acatcgggtc gattgccgca gacttgcggc gcactctgcc
     3841 cggaacccgg ttcgccgcgg aacgcgccac tgggttcgcg tacgaggcgc agttcgcctc
     3901 gggtcatccc gtgccggcgg cggcggcctg cgggcccatc gagtcgcaca ccgactcgcc
     3961 catcggcaag ctgctggccc ggaaccccgc cctgccggac gaggagttcc tggcccgggt
     4021 ggtcgagatc gtgggggacc gggcgatcgt ggcctactcc ggcgctggcg gactggccga
     4081 gatgtccgca cctggggtca ccaaagccgt tgccctgcaa cggttgacag gctcgctcgg
     4141 catcggtgcc acaggtgtct gggccttcgg cgacatgccg aacgacctgc ccatgatgcg
     4201 atgggccggg cgctcgatcg ccgtggccaa cgcccacccg gacgtgctcg cggtggcctc
     4261 ccacacctgt gccggcaacg acgaggacgg tgtcgccgag gtgctggagc ggctcgttcg
     4321 tgacatggac cggccgggct gaccggcgac ggcgggcgag gaaaggttcc tggacagacc
     4381 attgacgatc gcgacgaggc ccggcttaga tcgaggtatc ggtcgacgga gccgacgacc
     4441 gggtggatcg ggggcagcga ggtgagtggg gcgatggcac cggcaggcgc agaccgggtg
     4501 agcgacccgc tccccgatgc cgtccttcgg ccggtccgtg agggatatgc cttcgaggtc
     4561 accgtcgag
//
LOCUS       DATAIX010000270         2460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_274530, whole genome shotgun sequence.
ACCESSION   DATAIX010000270 DATAIX010000000
VERSION     DATAIX010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274530"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VHM65_04015"
     CDS             <1..189
                     /locus_tag="VHM65_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704900.1"
                     /translation="ESERLLRGYLSKALFDAGLYCRADDRGDPVVQLAPPLIIGQAEF
                     DDIESRLRSVLTGAEKHL"
     gene            complement(323..1762)
                     /locus_tag="VHM65_04020"
     CDS             complement(323..1762)
                     /locus_tag="VHM65_04020"
                     /inference="COORDINATES: protein motif:HMM:NF005102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX2704901.1"
                     /translation="MTIDPSPIWEPSSDHHTRTGKLYSEAAKDHLWMHFTRHSTYEDH
                     ESGGAGHFVPIITRGEGAYIWDDRGNKYLDGLSGLFTVQVGHGRTELAEAAMTQMNKI
                     AYFPLWSYAHPRAIELAERLAHYAPGDLNRVFFTTGGGEAVESAWKLAKQYFKLTGKP
                     LKHKVISRSIAYHGTPQGALSITGIPAAKTVFEPLVPGGHKVPNTNFYRAPEHLRDDP
                     KAFGRWAADRIAEAIEFEGPDSVAAVFLEPVQNSGGCFPPPPGYFDRVREICDEYDVL
                     LVSDETICAFGRIGDYFACNELGYVPDIITTAKGITSGYAPLGAMIVSDRLFEPYRTG
                     MNYFPHGYTFGGHPVSCAVAMANLDIFEREGLNQHVRDNAPKFRAALEKLLDLPIVGD
                     VRGEGYFYGIELVKDKATRETFNDLESERLLRGYLSKALFDAGLYCRADDRGDPVVQL
                     APPLIIGQAEFDDIESRLRSVLTGAENRL"
     gene            complement(1814..2314)
                     /locus_tag="VHM65_04025"
     CDS             complement(1814..2314)
                     /locus_tag="VHM65_04025"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX2704902.1"
                     /translation="MATRPTLVTRRSPARSGTVVVDAVAKAIIEALQVDGRQSYAAIA
                     AHVGLSEAAVRQRVQKLIDAGVMQIVAVTDPLQIGFRRQAMVGLKVEGDMLPVAEALS
                     AMPEVAYVVTTAGSLDLLCEVVCEDDDELLDLLTHRIRTLPGVRSTETFVYLKLNKQH
                     YNWGTR"
BASE COUNT          369 a          779 c          894 g          418 t
ORIGIN      
        1 gagtccgagc gcctcctgcg cgggtacctg tccaaggcgc tgttcgacgc gggcctctac
       61 tgccgcgccg acgaccgcgg cgacccggtc gtccagctcg cgcccccgct catcatcggc
      121 caggccgagt tcgacgacat cgagtcccgg ctacgctcgg tcctcaccgg tgccgagaag
      181 cacctctgag cggacggtgg gctggcccgg cataccggac caccacaacg gctcggtcat
      241 gccagtgcgc gcatcatgcc agtgccgagg tcatgccagt gcccgggccg ggtccggtat
      301 gccgggcgag cccaccggcc gctcagaggc ggttctcggc accggtgagg accgagcgaa
      361 gccgggattc gatgtcgtcg aactcggcct ggccgatgat gagcggaggc gcgagctgga
      421 cgaccgggtc gcctcggtcg tcggcgcggc agtagagccc cgcgtcgaag agcgccttgg
      481 acagatagcc gcgcaggagg cgctcggact cgaggtcgtt gaacgtctcg cgggtcgcct
      541 tgtccttgac gagctcgatc ccgtagaagt agccctcgcc gcggacgtca ccgacgatgg
      601 gcaggtcgag gagcttctcc agggctgcgc ggaacttggg ggcgttgtcg cggacgtgct
      661 ggttgaggcc ctcgcgctcg aagatgtcga ggttggccat cgcgaccgcg cacgagacgg
      721 ggtgcccgcc gaaggtgtag ccgtgcggga agtagttcat ccccgtgcgg tagggctcga
      781 agagccggtc gctgacgatc atggcgccca ggggggcgta gcccgacgtg atgcccttgg
      841 cggtcgtgat gatgtccggc acgtagccga gctcgttgca cgcgaagtag tcgccgatcc
      901 ggccgaaggc gcagatcgtc tcgtccgaga cgagcaggac gtcatactcg tcgcagatct
      961 cgcggacccg gtcgaagtac ccgggcggcg gcgggaagca gccaccggag ttctgcaccg
     1021 gctcgaggaa gaccgcggcg acactgtcgg gaccctcgaa ctcgatggcc tcggcaatgc
     1081 ggtcggcggc ccaccgcccg aaggccttcg ggtcgtcacg caggtgctcg ggcgcgcggt
     1141 agaagttggt gttcggcacc ttgtggccac cgggcacgag cggctcgaag acggtcttgg
     1201 ccgccgggat cccggtgatc gacaaggcac cctgaggggt gccgtggtaa gcgatggagc
     1261 gggagatgac cttgtgcttg aggggcttgc cggtcagctt gaagtactgc ttggccagct
     1321 tccaggcgct ctcgaccgcc tcgcccccgc cggtcgtgaa gaagacccgg ttgaggtcgc
     1381 cgggggcgta gtgcgcgaga cgctcggcca gctcgatggc gcgcgggtgg gcataggacc
     1441 acagcgggaa gtaggcgatc ttgttcatct gcgtcatggc ggcttcggcc agctcggtcc
     1501 ggccgtggcc gacctgcacg gtgaacaggc ccgagagccc gtcgaggtac ttgttgcccc
     1561 ggtcgtccca gatgtaggcg ccctcaccac gggtgatgat cggtacgaag tggcccgcgc
     1621 cgccgctctc gtggtcctca taggtcgagt gccgggtgaa gtgcatccac aggtggtcct
     1681 tggcggcctc ggagtagagc ttgccagtcc tggtgtggtg gtccgagctc ggttcccaga
     1741 taggggacgg gtcgatcgtc atggtggctt cctcgtgctg ttgtggacgt cgcgggtcac
     1801 gggccgtgcg ccgctaccgc gtgccccagt tgtagtgctg cttgttgagc ttgagataga
     1861 cgaaggtctc ggtgctgcgg accccgggca gggtccggat gcggtgggtc agcaggtcga
     1921 gcagctcgtc gtcgtcctca cagaccacct cgcagagcag gtcgaggctg cccgcggtcg
     1981 tgacgacata cgccacctcg ggcatcgcgc tcagtgcctc ggcgacgggg agcatgtcac
     2041 cctcgacctt gagccccacc atcgcctggc gccggaagcc gatctggagc gggtcggtga
     2101 cggcgacgat ctgcatgacg ccggcgtcga tgagcttctg cacgcgctgg cggaccgccg
     2161 cctcggagag gcccacatga gcggcgatgg cggcgtagga ctggcggccg tccacctgca
     2221 gcgcctcgat gatggccttg gccacggcgt cgacgaccac cgtcccgctg cgtgccgggg
     2281 agcgtcgggt cacgagggtg ggccgcgttg ccatctgctc atgctgcccg ggatcgggtg
     2341 gtctggcaag tgctgtgcaa cgcaaaccgc acctatcggt gtgtttgacg actgatcccg
     2401 cagatcttgg gggcgcaata tcgggaaacc cttgtggcga gccccctcgt gcctttagat
//
LOCUS       DATAIX010000271         1964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_14314, whole genome shotgun sequence.
ACCESSION   DATAIX010000271 DATAIX010000000
VERSION     DATAIX010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14314"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="VHM65_04030"
     CDS             <1..1275
                     /locus_tag="VHM65_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704903.1"
                     /translation="LGRLELLGAGVMGRRAPLAAAVLVAAGAVALTCAVTAAGLVAVG
                     QDASGSVALGLAWAGVGLTFVGVSAVAAQLAQTARGAGGITLAVLGAAYLLRAVGDTT
                     EGPLGAALVWLSPLGWPEKVSIYGENRYIAAIVPLAVTAGLLALADRLLARRDLGSGL
                     LATRVGPGRAAAALRSPAGLAWRLQRGSLIGWTVGYALLGLVLGAISQSITSMLDNPD
                     IVEMLRAMGGNAGTLVDLFVRTELVIAAIAAAGYGISAALRLRSEETTGNAEPVLAGA
                     VSRPRWLSSHLVVALSGSAWLMVVLAVTVALGARGGLTGPGTSVASMLGAAAVAVPAV
                     WVCVGLAAFVYGTVPRWVTALWAALVVFLVLGEFGRTLKLPDSLINVSPFPHIPPLPG
                     GALTLTPVVALSAVAATLGLVGYAAFRRRDIG"
     gene            complement(1311..>1964)
                     /locus_tag="VHM65_04035"
     CDS             complement(1311..>1964)
                     /locus_tag="VHM65_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX2704904.1"
                     /translation="VTLGVTPTHPATGFGYIRTGEALAGFPTARPVVRFVEKPDAASA
                     SAYVASGEFLWNAGMFVAQASVLLDVLASYHPVLARGARAIAADLARLGEVWPTMARI
                     SVDHAVAEPAAAEGRVAVVPATFDWDDIGDFAALGALLPERPGGVRVLGDAGRVMARD
                     ATGLVVTGTTRLVVVIGLDDVVVVDTPDAVLVVATGRAQDVKAVVDTLRSTGRTGLT"
BASE COUNT          219 a          727 c          730 g          288 t
ORIGIN      
        1 ctcggccggc tcgagctcct cggcgcaggc gtcatgggac ggcgcgcccc gctggcggcc
       61 gccgtgctcg tggcggcagg ggccgtggcg ctcacctgcg cggtgaccgc ggccgggctc
      121 gtcgccgtcg gccaggacgc gtccgggtcg gtcgcgctgg ggctcgcgtg ggcaggcgtg
      181 gggctgacct tcgtcggggt ctccgcggtc gccgcccagc tggcccagac cgcgcgcggc
      241 gcggggggga tcaccctcgc ggtcctcggc gcggcatacc tgcttcgggc cgtgggcgac
      301 accaccgagg gaccgctggg tgcagcgctg gtctggctct ctcccctcgg ctggccggag
      361 aaggtgtcga tctacggcga gaaccggtac atcgccgcga tcgtccccct ggccgtcacg
      421 gcgggtctgc tcgcgcttgc cgaccggctg ctcgcgcggc gggacctcgg ctccggcctg
      481 ctcgcgaccc gggtcggacc gggccgggcc gctgcggccc tgcgcagccc agcggggctt
      541 gcctggcgcc tgcagcgcgg ctccctcatc ggctggacgg tggggtacgc actgctcggg
      601 ctggtcctcg gggcgatctc gcagagcatc acctcgatgc tggacaaccc cgacatcgtc
      661 gagatgctcc gggcgatggg cgggaacgcc gggactctcg tggacctgtt cgtccggacc
      721 gagctggtca tcgcggcgat cgctgcggcg gggtacggca tctccgcggc acttcggctc
      781 cgctccgagg agacgaccgg gaacgccgag ccggtcctgg ccggcgcggt gagcagaccc
      841 cggtggctct ccagccacct cgtggtggcg ctgtccggca gcgcctggct catggtcgtt
      901 ctcgcggtca ccgtcgcgct cggcgcccgg ggtggcctga ccgggccggg gacctcggtg
      961 gcctcgatgc tcggcgccgc cgcggtggcg gtcccggcag tctgggtctg tgtcgggctg
     1021 gccgcgttcg tctacgggac ggtgccgcgg tgggtcacgg cactgtgggc ggcgctggtg
     1081 gtgttcctcg tcctcggaga gttcggccgc accctgaagc tccccgactc gctcatcaac
     1141 gtgtcgccgt tcccgcacat ccccccgctt ccggggggcg cgctgaccct gactcccgtc
     1201 gtcgcgctga gcgccgttgc cgcgaccctc ggcctcgtcg gttatgccgc atttcgccgc
     1261 cgcgacatcg gctgagggcc accgcgacat cggctgaggg cagccggacc tcaggtcagg
     1321 ccggtgcggc cggtggacct gagcgtgtcg acgacggctt tgacgtcctg agcgcggccc
     1381 gtggcgacga caagcacggc gtcgggcgtg tcgacgacga cgacgtcgtc gagcccgatg
     1441 acgacgacga gacgcgtcgt gcccgtgacg accagcccgg tcgcgtcccg cgccatgacc
     1501 cggcccgcgt cgccgagcac gcgcacgcca ccgggccttt cgggcagcag cgcaccgagg
     1561 gccgcgaagt ccccgatgtc gtcccagtcg aacgtcgccg ggacgaccgc gacgcgcccc
     1621 tcggcggcgg ccggctcagc caccgcgtgg tcgacggaga tcctggccat cgtcggccac
     1681 acctccccca gccgcgcgag gtcggccgcg atcgcgcgtg cccccctcgc cagaacaggg
     1741 tggtatgacg cgagcacgtc gagcagcacg ctcgcctgcg cgacgaacat gccggcgttc
     1801 cacaggaact cgccggatgc gacatacgcc gacgcacttg ctgcgtctgg cttctcgacg
     1861 aaccggacga ccgggcgggc cgtggggaag ccggcgagcg cctcccccgt gcggatgtac
     1921 ccgaacccgg tggccggatg cgtcggcgtg accccgagtg tgac
//
LOCUS       DATAIX010000272         3550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_14403, whole genome shotgun sequence.
ACCESSION   DATAIX010000272 DATAIX010000000
VERSION     DATAIX010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14403"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..1075)
                     /locus_tag="VHM65_04040"
     CDS             complement(242..1075)
                     /locus_tag="VHM65_04040"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2704905.1"
                     /translation="MSWKKILIVPNVMRLQRRAPKDVSTRWDEYWAGVRETGSHGDVL
                     WDPDSPVEAQRYLDELRRHSDLDLPVVDVGCGNGRITRMLAATFPSAVGVDLAPHAIS
                     RAEEESRGISNLTFRALDMTAPAAGRQLAAELGEVNVLVRGVFHILDRSQRLAMAANL
                     RDLLGRQGTLLLAETNFPGSLLAYLEYLGATSTWLPKPLERAITTGISRPSRFGEAQL
                     AACFPADQWQLLLAETTCIETVPMRGLDGPETLPGFLAVLQTRPREMSESSPAASAPR
                     S"
     gene            complement(1094..2704)
                     /locus_tag="VHM65_04045"
     CDS             complement(1094..2704)
                     /locus_tag="VHM65_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2704906.1"
                     /translation="MNHDDARLDQLVDFVVQIAAGDLAARLEPSGVADSIEAVAVGLN
                     MLAEELEVLYAGLEQHVAERTASLEQAQAALRSQALTDALTGLANRTLLGDRIGQALA
                     RAQRGALPPTLLALDLDGFKLINDSLGHAGGDTVLVEVARRLETVARRTDTVARFGGD
                     EFAILITDATSDAALRIAERALDVLRRPMRVGDQLTSAGASIGVCFGSRGKTPDSLLR
                     DADTAMYAAKTRGRNNVQVFKPEMHAAALARVQIADELHTALRTHELTVHFQPLVDLA
                     SARVVGAEALVRWNHPRRGLVRPVEFVPVAEETGLIVEIGQRVTVEAIRQLAKWQKEL
                     VLPESFRLHVNVSPVEFRRASFAPFVRETLQRYGVPAASLVLEITENGLMTDEGEIAQ
                     TLHDLRSGGVGLAIDDFGTGYSSISYLRRLPVDSVKIDRSLVEGVDTDPQQRNLVAAV
                     VALIDAVNLTPIAEGVETAGQATSLQALGCVYGQGYHFSRPVSAGEMTGLLRVDHVQR
                     AGPEQPDTIGRQRPSPVPTRQTGNVPSL"
     gene            complement(2694..>3550)
                     /locus_tag="VHM65_04050"
     CDS             complement(2694..>3550)
                     /locus_tag="VHM65_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX2704907.1"
                     /translation="PGPRTGRRRRWEVMGAALASRPVGQEEGMPDIAGVPVEELSTDG
                     MIVSVNDAYCQMLGREPGELLGHRPTEFTHPDDRAATAESVTSVATAPHDDVRFEKRY
                     LRADGTVVWARVSSTWLPVEHRVVGHVVEINEDIEARNAARAAQLRLTALVEEVVAAS
                     VDSGQVTAELDEAGFVRLSWEPGVKITGDLAREAVAALERVCGGRPRPMLVDMTSTGA
                     LTRGARVVFAEKSAASAIALLGKSAVDRVIANFALGVSPVPVPTRFFTVESVALAWLL
                     KSGDEREP"
BASE COUNT          541 a         1254 c         1207 g          548 t
ORIGIN      
        1 ggtcggcccg ccgaggcccc gtcgtggctc gtgagacggg acggactggt ctgcagccgt
       61 cgtccccggc tcggcatcgg aggcatgcgc agcaggcgca acgggcgccg ggactgccgg
      121 cccatgggcg tcctgcggtg cagggctcat ccactcagcc tcgccgggtc gctgcaaccg
      181 agggaggcca taggtcccga ccacttgccg ggagcctccg cgtcttcccc tgttcatcgg
      241 actacgacct cggcgcgctg gccgccgggc tggactcgct catctctcgc ggccgggtct
      301 ggagcacggc gaggaacccc gggagggtct ccggcccgtc caggccccgc atgggcaccg
      361 tctcgatgca ggtcgtctcg gcgaggagga gctgccactg atcggcaggg aagcaggccg
      421 ccagctgcgc ctccccgaag cgcgatggtc gtgagatgcc ggtggtgatc gcccgctcga
      481 ggggtttcgg cagccaggtc gacgtcgctc ccaggtactc gaggtaggcg agcaggctgc
      541 ccgggaagtt cgtctccgcc aggagcagcg tgccctgccg gccgagcagg tctcgcaggt
      601 tggcggccat ggcgaggcgt tgcgatctgt ccaggatgtg gaacacacca cggacgagga
      661 cgttcacctc tcccagctcg gcggccagct gccgtccggc tgccggtgct gtcatgtcca
      721 gggcacggaa ggtgaggttg ctgatgccgc gggactcctc ctcggcccgg gagatggcat
      781 ggggggcaag gtcgacgccg accgccgacg gaaacgtcgc cgccagcatc ctggtgatcc
      841 ggccgttgcc gcagcccacg tcgactaccg ggaggtcgag gtcactgtgc cgacgcagct
      901 cgtcgaggta gcgctgggcc tccaccggac tgtccggatc ccagagcacg tccccgtgcg
      961 aacccgtctc ccgcacgcct gcccagtact cgtcccagcg cgtgctcacg tccttgggcg
     1021 cacgccgttg cagcctcatc acgttgggga cgatgaggat cttcttccac gacaccagga
     1081 cctcctgcgg cgctcaaagg gagggcacgt tgccggtttg ccgggtaggt accggtgatg
     1141 gacgctgccg gccgatcgta tcgggctgct ccgggccggc gcgctgcaca tggtcgacgc
     1201 ggagcagccc ggtcatctcg ccggctgaga ctggtctgct gaagtggtag ccctgcccgt
     1261 agacacatcc cagggcctgc agcgaggtgg cctgcccggc ggtctcgacc ccttcggcga
     1321 tgggggtgag gttcaccgcg tcgatcagcg cgacgaccgc cgcgaccagg ttgcgttgct
     1381 gagggtcggt gtcgacgccc tcgaccagtg agcggtcgat cttgacggag tcgacgggca
     1441 gccggcgcag atagctgatc gaggagtacc ccgtaccgaa gtcgtcgatc gccaggccga
     1501 cgcctccgga ccgcagatcg tgcagggtct gggcgatctc accctcatct gtcatcagcc
     1561 cgttctcggt gatctccagc accagactgg ccgctgggac cccgtaccgt tgcagcgtct
     1621 cccggacgaa aggagcgaac gatgcccgtc ggaactcgac aggagagaca ttgacgtgca
     1681 accggaacga ctccggcaac acgagctcct tctgccactt cgccagctgg cggatcgctt
     1741 cgaccgtgac ccgctgcccg atctcgacga tgagccccgt ctcctcggcg acggggacga
     1801 actccaccgg ccgcaccagc ccgcgtcgcg ggtggttcca cctcaccagg gcctcggcgc
     1861 cgacgactct ggcgctggcc agatcgacca acggctggaa gtgcaccgtc agctcatgtg
     1921 tcctgagcgc ggtatggagc tcgtcggcga tctggacgcg cgcgagcgcg gcggcgtgca
     1981 tctccggttt gaagacctga acgttgttcc ggccccgcgt cttggccgcg tacatggccg
     2041 tgtccgcgtc ccgcagcagg gagtccggcg tcttaccgcg agagccgaag cacaccccga
     2101 tactggcgcc ggccgaggtc agctggtcgc cgaccctcat cggacggcgc aacacgtcca
     2161 gggcgcgttc ggcgatgcgt agcgctgcgt cggaggtcgc gtccgtgatc aggatggcga
     2221 actcgtcccc gccgaaccgg gccaccgtgt cggtccgccg ggcgacggtc tccagtcgtc
     2281 gggccacctc cacgaggacg gtgtcgccgc cggcatgacc gaggctgtca ttgatcagct
     2341 tgaacccgtc caggtcgagc gcgagcagag tcggcggcag agcgccccgt tgggcgcggg
     2401 cgagcgcctg cccgatgcgg tcgccaagca gtgtcctgtt ggccagaccg gtcagggcat
     2461 cggtaagcgc ctgggaccgc agcgccgctt gcgcctgctc gagcgaggcg gtgcgctccg
     2521 ccacatgttg ctcgagtccg gcatacagca cctccagctc ctcggcgagc atgttcaggc
     2581 ccacggccac tgcctctatg gagtcggcta cccccgacgg ctcgagcctc gcggccaggt
     2641 caccagcggc gatctgcacc acgaagtcga ccagctggtc caggcgcgca tcatcatggt
     2701 tcacgttcgt cgccggactt cagcaaccag gccagcgcca cggactcgac ggtgaagaac
     2761 cgggtgggaa cgggcaccgg gctcaccccg agggcgaagt tcgcgatgac cctgtcgacg
     2821 gccgacttgc ccagcagggc gatcgccgag gcagcggact tctccgcgaa gaccaccctg
     2881 gccccccgcg tcaaggctcc tgtcgaggtc atgtccacca gcatcgggcg cggtcgccct
     2941 ccgcagaccc gctcgagcgc cgcgacagcc tcacgggcaa ggtcgccggt gatcttcacc
     3001 cccggctccc aggacaaccg gacgaagccg gcctcatcca gctccgcagt gacctgacca
     3061 gagtcgaccg aagccgccac cacctcctcg acgagcgcgg tcagccgcag ctgggcggcc
     3121 cgggcagcat tacgggcctc gatgtcctcg ttgatctcga cgacgtggcc gacgacacgg
     3181 tgctcgacgg ggagccaggt gctgctgacg cgggcccaca cgacggtacc gtcggcgcga
     3241 aggtaacgct tctcgaaccg cacgtcgtca tggggcgccg tggccaccga ggtgacgctc
     3301 tcggcggtgg cggccctgtc gtcagggtgg gtgaactccg tcgggcggtg tccgagcagc
     3361 tcgccgggct cccggcccag catctgacag taggcatcgt tgacggacac gatcatgccg
     3421 tccgtggaca gctcttccac cggcacccca gcgatgtcgg gcatcccttc ctcctgaccg
     3481 accggacggc ttgcgagagc cgcgcccatg acctcccagc gtcgccgccg accggttctc
     3541 ggtccagggg
//
LOCUS       DATAIX010000273         3500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_44699, whole genome shotgun sequence.
ACCESSION   DATAIX010000273 DATAIX010000000
VERSION     DATAIX010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44699"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /gene="atpD"
                     /locus_tag="VHM65_04055"
     CDS             <1..1330
                     /gene="atpD"
                     /locus_tag="VHM65_04055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX2704908.1"
                     /translation="LTTEVELAGVTKTLNLEVAQHIGDSMVRAISLQPTDGLVRGTAV
                     HDTGGPITVPVGDVTLGHVFNTTGDCLNLKEGERLDVTERWGIHRQAPAFDQLESKTQ
                     MFETGIKVIDLLTPYVQGGKIGLFGGAGVGKTVLIQEMIARVARDHGGVSVFAGVGER
                     TREGNDLIVEMDEAGVLGQTALVFGQMDEPPGTRLRVALSALTMAEYFRDVQKQDVLL
                     FIDNIFRFTQAGSEVSTLLGRMPSAVGYQPTLADEMGVLQERITSTRGHSITSMQAIY
                     VPADDYTDPAPATTFAHLDATTELSREIASLGIYPAVDPLASTSRILDRRYITEAHYS
                     TAVRVKSILQRNKELQDIIAILGIDELSEEDKILVNRARRIQRFLSQNIYVAKQFTGI
                     EGSTVSLGDTVEAFTKICDGDYDHMGEQAFYMCGGLDDVERQWAEIQKNL"
     gene            complement(1353..2264)
                     /locus_tag="VHM65_04060"
     CDS             complement(1353..2264)
                     /locus_tag="VHM65_04060"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX2704909.1"
                     /translation="MAILSALLTVVLPVLIVAGVGALLSRRFELDQTTITKLSLNGLT
                     PALALHTLLTTAISPGTGTRLAAAYLVVSLAAVALAGLATPGMTTRTRRAVMACAAIG
                     NNGNMGLPISLFALGRAGLDQSVVIFLCSVVLTFTLGPLLYGSTDGPLGALRAVVRLP
                     VLWAMAVAVGIRLLALPVPLGVMRGIELLAQATLPMILLALGIALGRGGAIGFGRPVL
                     TAVGLRVLVMPVAGLGVGWLFGLRGLPLEALVLSGAMPTAVNAYLLAREYDADAVTVA
                     DAVTLSTLASIASAAVVTALLPAIAAL"
     gene            2600..2866
                     /locus_tag="VHM65_04065"
     CDS             2600..2866
                     /locus_tag="VHM65_04065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009977.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX2704910.1"
                     /translation="MSELQVEMVAADRLVWSGTASMVRARSVDGDLGILPGHAPLLAL
                     LGSGEVSVKAVSGPDVSATIDTGFLSVEHDRVTIVAERVTTSAG"
     gene            2871..3308
                     /locus_tag="VHM65_04070"
     CDS             2871..3308
                     /locus_tag="VHM65_04070"
                     /inference="COORDINATES: protein motif:HMM:NF022199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2550 family protein"
                     /protein_id="HEX2704911.1"
                     /translation="MPFALVSAEVVIGCAVVLVALFLAWTFVRRSVICRGRTVLVCGW
                     RGADVSVWQAGLLGLEGEQLEWFPLSGVRIRPRFCWDRTGLEVDVPRPVPSTTGLGID
                     GSAFALTCRYGDTRFDITLDDPSYTALRSWLDSVPPGYNVNVA"
     gene            complement(3312..>3500)
                     /locus_tag="VHM65_04075"
     CDS             complement(3312..>3500)
                     /locus_tag="VHM65_04075"
                     /inference="COORDINATES: protein motif:HMM:NF014033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704912.1"
                     /translation="ALERYGAQPGTERGAGGINPLTATYLNRLSDLLFILARVANHPI
                     GGDVLWQPGGGRVAPPRG"
BASE COUNT          545 a         1243 c         1199 g          513 t
ORIGIN      
        1 gctcacgacc gaggtcgagc tcgccggtgt gacgaagacc ctcaacctcg aggtcgccca
       61 gcacatcggc gacagcatgg ttcgcgccat ctcgctgcag ccgaccgacg ggctcgtccg
      121 tggcacggcc gtccatgaca ccggcggtcc gatcaccgtg cccgtcggcg acgtgacgct
      181 cgggcacgtc ttcaacacga cgggcgactg cctcaacctc aaggagggcg agcgtctcga
      241 cgtcaccgag cggtggggca tccaccgcca ggcgccggcg ttcgaccagc tcgagtccaa
      301 gacgcagatg ttcgagaccg ggatcaaggt catcgacctg ctcaccccct acgtccaggg
      361 tggcaagatc ggcctcttcg gcggggccgg ggtcggcaag accgtcctca tccaggagat
      421 gatcgcccgg gtcgcgcgtg accatggcgg tgtctccgtg ttcgccggcg tcggcgagcg
      481 cacccgtgag ggcaacgacc tcatcgtcga gatggacgag gccggcgtgc tcgggcagac
      541 cgccctcgtc ttcgggcaga tggacgagcc gccgggcacc cggctccggg tggccctgtc
      601 ggccctgacg atggccgagt atttccgcga cgtccagaag caggacgtgc tcctcttcat
      661 cgacaacatc ttccgcttca cccaggctgg ctcggaggtc tcgaccctgc tcggccggat
      721 gccgagcgcg gtgggctacc agccgaccct tgccgacgag atgggcgtgc tccaggagcg
      781 gatcacctcg acccgtggcc actcgatcac ctcgatgcag gcgatctacg tccccgccga
      841 cgactacacc gacccggcgc ccgcgacgac cttcgcccac ctcgacgcga cgaccgagct
      901 ctcccgtgag atcgcgtcgt tggggatcta cccggccgtg gacccgctcg cctcgacctc
      961 gcggatcctc gaccgccggt acatcaccga ggcgcactac tcgacggccg tgcgggtcaa
     1021 gtcgatcctg cagcgcaaca aggagctcca ggacatcatc gcgatcctcg ggatcgacga
     1081 gctctccgag gaggacaaga tcctcgtcaa ccgggcccgg cggatccagc gcttcctctc
     1141 gcagaacatc tacgtcgcca agcagttcac cggaatcgag ggctcgacgg tgtcgctcgg
     1201 cgacaccgtc gaggccttca cgaagatctg tgacggcgat tacgaccaca tgggtgagca
     1261 ggcgttctac atgtgtggcg gcctcgacga cgtcgagcgc cagtgggccg agatccagaa
     1321 gaacctgtag ccctgcgtcg cgggcctggc ggctacagcg cggcgatggc cggcagcagt
     1381 gctgtgacga ccgccgctga ggcgatgctc gccagggtgc tcagcgtcac cgcgtcggcg
     1441 acggtgacgg cgtccgcgtc gtactcccgg gcgagcaggt aggcgttgac cgcggtcggc
     1501 atcgcaccgg agagcacgag tgcctccagc gggagaccac gcagcccgaa gagccagccg
     1561 accccgaggc ctgcgaccgg catgaccagg acccgcagcc ccaccgcggt gagcacgggg
     1621 cgcccgaacc cgatcgcgcc gccgcggccg agcgcgatgc cgagggcgag caggatcatc
     1681 ggcagggtgg cctgggcgag cagctcgatc ccgcgcatga cccccagggg cacggggagg
     1741 gcgagcaggc ggattcctac ggccaccgcc atggcccaga gcaccggaag ccgcacgacc
     1801 gcccgcagcg cgccgagcgg cccgtcggtc gagccgtaga gcagcgggcc gagggtgaag
     1861 gtcaacacga ccgagcacag gaagatcacg acactctggt cgagcccggc ccgtccgagc
     1921 gcgaacagcg agatcggcag ccccatgttg ccgttgttcc cgatcgccgc gcacgccatg
     1981 accgcgcggc gggtgcgtgt cgtcataccg ggtgtcgcga gtcccgcgag ggcgaccgcg
     2041 gccagggaca cgacgaggta tgccgccgcc agccgggtac ccgtgccggg ggagatcgcc
     2101 gtggtcaaga gcgtgtgcag tgccagggcc ggcgtcagcc cgttgaggct gagcttggtg
     2161 atcgtcgtct ggtcgagctc gaaccggcgg ctcagcagcg ccccgacgcc ggcgacgatg
     2221 agcacgggga gcaccacggt gagcagggcc gagaggatcg ccacgcccgg acgctacccc
     2281 ccacggtcgg ggcacgggag gcccgcccac ctgccggtcg cgcgccctgc cgtgtcgggg
     2341 gcgcggtcca ctaccatcgc tgcaccccga caacgaggac ttcctgcacc ccttcatgct
     2401 caaaccgggg tgtcctgctc accccgttac acaaatggcc ctcatgtgcc cggtcacgac
     2461 cagtgccgac gtcgctcgtc accacgaggt cctggcggcg ctcgacgagc tggtggggtg
     2521 agccacactc agggcagccc cggcccgagg ccgagccgtg cccgctagac tgtccggact
     2581 atctgcagcg gagggcatcg tgagtgagct tcaagtcgag atggttgctg ccgaccgcct
     2641 ggtctggtcc ggcacggcgt cgatggttcg cgcccgttcg gtcgacggcg acctgggcat
     2701 cctgcctggt cacgctcccc tgctcgcgct cctcggctcg ggcgaggtgt cagtcaaggc
     2761 ggtgtccgga cctgacgtct cggcgacgat cgacaccggc ttcctctcgg tcgagcacga
     2821 ccgagtgacg atcgtggcag agcgcgtcac cacctccgcg ggttgacctc gtgccgttcg
     2881 cgctggtgtc cgcagaggtg gtcatcggct gcgcggtggt gctcgtggcg ctcttcctcg
     2941 cctggacctt tgtccgtcgt tcggtgatct gccgcggccg caccgtgctg gtctgcggct
     3001 ggcgcggcgc tgacgtgtca gtctggcagg ccgggctgct gggcctcgag ggcgaacagc
     3061 tggaatggtt ccccctgagc ggggtaagga tccggccgcg cttctgctgg gatcggaccg
     3121 ggctggaggt cgacgtgccg cggccggtcc cctcgacgac gggcctgggc atcgacgggt
     3181 ccgcgttcgc gctgacctgc cgatacggcg atacccgctt cgacatcacc ctcgacgacc
     3241 cgagctacac cgcactacgg tcctggctcg actcggtccc gccgggctac aacgtcaacg
     3301 tggcctgagc ctcatccccg cggcggggcg acccggccac cgccgggctg ccacaggacg
     3361 tccccgccga tcgggtggtt ggccacccgg gccaggatga acagcaggtc cgagagccgg
     3421 ttgagatagg tcgccgtgag cgggttgatc ccgcccgcac cccgctcggt gccaggctgc
     3481 gcgccatacc tctccagtgc
//
LOCUS       DATAIX010000274         1667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_244794, whole genome shotgun sequence.
ACCESSION   DATAIX010000274 DATAIX010000000
VERSION     DATAIX010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244794"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VHM65_04080"
     CDS             complement(<1..816)
                     /locus_tag="VHM65_04080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005926.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX2704913.1"
                     /translation="MTVWDEVIGQSQTVRTLSGAVADVNLMTHAWLLTGPPGSGRSTA
                     ARAFAAALECPANGCGQCRECRTALDGTHADVQLVATEGLSIKVDQARELVQEAALRP
                     SVGAWRVIVVEDADRLTERAADALLKALEEPTARTVWVLCAPSVEDVIITIRSRTRHV
                     RLRTPPVKAVAELLERRDGIDPVMALFAARAAQSHVGLARRLARDEGARIRRHDVIAL
                     PTRVRSVGDAIGAAADLASIADEESGAASPERDAGERARLLETLCADASARTEP"
     gene            complement(813..1484)
                     /gene="tmk"
                     /locus_tag="VHM65_04085"
     CDS             complement(813..1484)
                     /gene="tmk"
                     /locus_tag="VHM65_04085"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX2704914.1"
                     /translation="MTVTTADQRTRRGVFVALEGGDGAGKSTQATLLRDWLVARGRRV
                     VVTREPGGTQVGQQIRQVLLHGDHVAPRADALLFAADRGQHVETLVRPALERGHDVIT
                     DRYLDSSVAYQGAGRDLGADEVRRLSLWAVRDLLPDLTVLLDVSSTTGRSRRGETHDR
                     LEREPDGFHERVRRHFLDLAAAGPSRYLVLDAGRPEHELARAIQERMSALLAAREPEP
                     EPEPV"
BASE COUNT          213 a          624 c          617 g          213 t
ORIGIN      
        1 gggctcggtc cgcgcggatg cgtcggcgca gagggtctcg agcagcctcg cccgctcccc
       61 cgcgtctcgc tcgggcgacg ccgcgccgga ctcctcgtcg gcgatcgagg ccaggtcggc
      121 ggccgcgccg atggcgtcac cgaccgaccg gacccgcgtc ggcagcgcga tgacgtcatg
      181 gcgccggatc cgggcgccct cgtcccgggc gagccgacgg gccagcccca cgtggctctg
      241 tgccgcgcgg gcggcaaata gggccatgac ggggtcgatc ccgtcgcgtc gctcgagcag
      301 ctcggccacg gccttgaccg gtggggtgcg cagccggacg tggcgggtcc ggctccggat
      361 cgtgatgatg acgtcctcga ccgagggcgc gcagagcacc cagacggttc tcgcggtggg
      421 ctcctcgagc gccttgagca gggcgtcggc cgcgcgctcg gtcaggcggt cggcatcctc
      481 gacgacgatg acccgccagg caccgaccga tgggcgcagc gccgcctcct ggacgagctc
      541 gcgcgcctgg tcgaccttga tcgacaggcc ttcggtggcg accagctgga cgtcggcgtg
      601 ggtgccgtcg agcgcggtcc ggcactcgcg gcactgtccg cacccgttgg ccgggcactc
      661 caaggcggcc gcgaacgccc gcgcggcagt cgaacgcccc gaccccggcg gcccggtgag
      721 cagccaggcg tgggtcatga ggttaacgtc ggctaccgcc ccggacaagg tccgcacggt
      781 ctggctctgc ccgatgacct cgtcccagac cgtcataccg gctccggctc cggctccggc
      841 tcgcgcgcgg cgagcagggc agacatgcgc tcctgtatgg cgcgcgccag ctcgtgctcg
      901 gggcggccgg catcgagcac aaggtagcgc gacgggccgg ccgcagcgag gtcgaggaag
      961 tggcggcgca ctcgctcgtg gaagccgtcg ggctcgcgct cgagccggtc gtgggtctcg
     1021 ccccgccgcg agcgcccggt cgtgcttgac acgtcgagca gcacggtgag gtcgggcagc
     1081 aggtcgcgca ccgcccagag ggacaggcgg cgcacctcat cggccccgag gtcgcgcccg
     1141 gcgccctggt aggcgaccga cgagtcgagg tagcggtcgg tgatgacgtc gtgacctcgc
     1201 tccagggcgg gccggaccag ggtctccaca tgctggcccc ggtcggcggc gaagagcagc
     1261 gcgtcggctc gcggcgcgac atggtcgccg tggagcagca cctgccggat ctgctgaccg
     1321 acctgcgtcc cgccgggctc gcgggtcacg acgacccggc ggccgcgcgc cacgagccag
     1381 tcccgcagca gcgtggcctg ggtcgacttg ccggcaccgt cgccgccctc gagcgccacg
     1441 aacaccccgc gccgggtgcg ctggtcggcg gtcgtgacgg tcacgggacg agcctaggtg
     1501 agagatctgt catgacggac gccacccccg gccgcctacg atgacgccat ggtctccctg
     1561 ctcaggggac gcgcggtcct cgtcgccgcc tccctgctca cgtgcgcggc ctgcacgagc
     1621 gacggcagcc agccgaccgc catacccacg actttgccca gccccgc
//
LOCUS       DATAIX010000275         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_104273, whole genome shotgun sequence.
ACCESSION   DATAIX010000275 DATAIX010000000
VERSION     DATAIX010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104273"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1428
                     /locus_tag="VHM65_04090"
     CDS             91..1428
                     /locus_tag="VHM65_04090"
                     /inference="COORDINATES: protein motif:HMM:NF027593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4921 family protein"
                     /protein_id="HEX2704915.1"
                     /translation="MTEPTPGPVPWREPLVCMADGTVKQKSPFTGLEVWTIPGRAERP
                     IGGVPTERHHIDIATEGRVCAFCERCYLQTPPEKARIIRDPGGGWRTIENVPAAEVES
                     TVAEFRLVPNLFSIVSLRYWTANYGYHLSPEEEARRAAYLASPDGLAHILALVRHKLR
                     LRGIDDGEWAAMSSEERLSHAPTIFGGGHDLVVARRHHVEGAKYEDDLASAGTLTPDE
                     HGQYVALSVIAMRNLFRENPFVRYVSAFQNWLRPAGASFDHLHRQLVAIDEYPARAEF
                     EIPRLRRDPDLYNTMGVDYAVSQRLIVAENETAIAYAGFGHRYPTLMVCSKSEHMLPW
                     DHTPAEIRGMSDLLHACHAATGALVPTNEEWHYRPPLITDAMPWRFLLKWRLSTSAGF
                     EGGTGIYINTIDPWQLRERVVRRLVALQRSGRLAAMSVGDACAVPRGVLRYRG"
     gene            complement(1560..>2436)
                     /locus_tag="VHM65_04095"
     CDS             complement(1560..>2436)
                     /locus_tag="VHM65_04095"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX2704916.1"
                     /translation="PGTESTRGAAVTNRPWDHWPWRALWPRQDDPAIGRQGRSPDMGR
                     LDGRVALVTGGASGIGKATALRLASEGASLVVTDITDEAGAGVAAQIGDAGGRAVFVH
                     HDVTSESDWLTAVSLAAEEFGGLDILVNNAGTGDLATIEETSLLDWERTIAIDQTGVF
                     LGMKTASELLAESGHGSVINISSIFGTIGGFGTSPAYHAAKGAVRTLTKNVALHWAAA
                     GVRVNSIHPGFILTPLLDPAMGTDVEELMVSTTPMGRLGQPEEVAAGVAYLASDDASF
                     VTGSELYIDGGYIAR"
BASE COUNT          357 a          875 c          828 g          376 t
ORIGIN      
        1 ggtctccggg ctagtctgaa gcctctgtga aggcgcctgc ggcggctcga ccgaccggcg
       61 cgctcacccg gccgaccata ggctgaggcc atgaccgaac cgacgccggg gcccgtgccc
      121 tggcgtgaac cgctcgtctg catggccgac ggaacggtga agcagaagag ccccttcacc
      181 ggcctggagg tctggacgat tccggggcgc gccgagcgac ccatcggggg ggtgcccacc
      241 gagcgccacc acatcgacat cgccaccgaa ggccgggtct gcgcgttctg cgagcgctgc
      301 tatctgcaga ctccgcccga gaaggcccgg atcatccggg acccgggcgg gggctggcgc
      361 acgatcgaga atgtccctgc cgcggaggtc gagtccacgg tggccgagtt ccggctcgtg
      421 cccaacctct tctcgatcgt ctcgttgcgc tactggaccg ccaactacgg ctaccacctc
      481 tcgccggagg aggaggcgcg gcgcgcggca tacctcgctt ccccggacgg cctcgcgcac
      541 atcctcgcgc tcgtgcggca caagctgcgt ttgcgcggca tcgacgacgg ggagtgggcg
      601 gccatgtcct ccgaggagcg gctcagccac gctccgacca tcttcggcgg gggccatgac
      661 cttgtcgtcg cgcgacgcca ccacgtggag ggggccaagt acgaggacga cctcgcctcg
      721 gcggggaccc tgaccccgga cgagcatggg cagtatgtcg cactctcggt catcgcgatg
      781 cggaacctgt tccgggagaa cccgtttgtt cgctacgtct cggcgttcca gaactggctg
      841 cggccggcgg gggcgtcgtt cgaccacctc caccgtcagc ttgttgccat cgacgagtat
      901 cccgctcggg ccgagttcga gatcccccgc ctgcgccggg accctgacct ctacaacacc
      961 atgggtgtcg actatgccgt gtcgcagagg ctcatcgtgg ccgagaacga gacggccatc
     1021 gcgtacgccg ggttcggcca ccgctacccc acactgatgg tgtgctccaa gagcgagcac
     1081 atgctgccgt gggaccacac cccggcggag atcaggggga tgtcggacct gctccacgcc
     1141 tgccacgccg cgacgggtgc tctggtgccc acgaacgagg agtggcatta ccgaccaccg
     1201 ctcatcaccg acgccatgcc gtggcggttc ctgctcaagt ggcggctgtc gacctcggcc
     1261 ggcttcgaag gggggacggg tatatacatc aacaccatcg acccgtggca gctgcgcgag
     1321 cgggtggtcc gccggctcgt ggctctgcag cgctcggggc ggctggccgc catgtcggtc
     1381 ggggatgcgt gcgccgtgcc ccgcggcgtt ctgcgctacc gggggtgacc gagtctgtgc
     1441 gcacccgacc gatgggcccg cagcgcccgc accagcatcc ccagccgcag ggccccggtc
     1501 acgacggcac ggctgcaggc gtcgcgtcgt gacccaaccc ctgcgccggc cccgcagcgt
     1561 caccgggcga tgtagccgcc gtcgatgtag agctcggaac ccgtgacgaa cgacgcgtcg
     1621 tcgctcgcga ggtaggccac gcctgccgcg acctcctcgg gctggccgag gcggcccatc
     1681 ggggtcgtcg acaccatgag ctcttcgacg tcggtgccca tggccggatc gagcagcggc
     1741 gtgaggatga accccggatg gatcgagttc acccggacac ccgcagcggc ccagtgcagg
     1801 gcgacgttct tggtcagcgt gcggacggca cccttggccg cgtggtaggc cggcgaggtc
     1861 ccgaaccccc cgatcgtgcc gaagatcgag ctgatgttga tgaccgagcc gtggccggac
     1921 tcggccagga gctcggacgc cgtcttcatc ccgaggaaga ctccggtctg gtcgatggcg
     1981 atcgtgcgct cccagtcgag caggcttgtc tcctcgatcg tcgcgaggtc acccgtaccg
     2041 gcattgttga cgaggatgtc gaggcccccg aactcctcgg ctgccagcga gacggccgtg
     2101 agccagtcgc tctcggacgt cacgtcgtgg tggacgaaga ccgccctccc gccggcatcg
     2161 ccgatctggg ccgccacgcc ggccccggct tcgtcggtga tgtcggtgac gacgagcgac
     2221 gccccttcgc tcgcgaggcg caacgccgtg gccttcccga tcccgctggc tcccccggtt
     2281 acgagcgcaa cgcgcccatc gagcctgccc atgtccggac tccgtccttg tcggccgatg
     2341 gccggatcgt cctgccgcgg ccacagggct cgccagggcc aatggtccca gggccggttg
     2401 gtgaccgcgg ctccccgcgt agactcggtc cccggc
//
LOCUS       DATAIX010000276         2584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_214921, whole genome shotgun sequence.
ACCESSION   DATAIX010000276 DATAIX010000000
VERSION     DATAIX010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214921"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="VHM65_04100"
     CDS             complement(<1..1227)
                     /locus_tag="VHM65_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEX2704917.1"
                     /translation="MSDQVRADAVDPGRPSRRAVLGFGAALGGGAGLCALAGCSSSDT
                     TSTGATGSAATPAPAAAPAIAAEPFYGPHQSGIATTPQTNLVLVGLDLLPGAGRDELR
                     RLLRLWTHDAARLTQGQPTLQDTAPDLSGPPARLTVTVGLGPQAVAAARPTEAPPAWL
                     APLPAFSVDRPDPAYTGGDVLLQVCGDDPITLAHAVRSLLLDAVGLARQRWVQRGFRR
                     PAGSTASMRTLMGQIDGTVNPKPATPEFDDLVWVGDEGPSWLRGGTGFVVRRIRMNLA
                     AWDLLDPPAKEEVIGRRLSSGAPLTGTRETDPADLGATSATGLSVIPDFAHIRHAAAD
                     LPRERFLRRPYNYDEGATGAPSEDSGLVFITFQRDITEQFVPVQRRLAAKDLLNMWTT
                     PVGSAVFAILPGAADGE"
     gene            complement(1237..1809)
                     /locus_tag="VHM65_04105"
     CDS             complement(1237..1809)
                     /locus_tag="VHM65_04105"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX2704918.1"
                     /translation="MNSISPSRRALVAAALVPTVLAAFTACGSGTTSAGATSPVSGSS
                     STTSMAGTEREIGLTSAWVKAADSGMTAAFGMIVNESHEEVTLVKAEFAGAGMVQLHE
                     TVKSGDTMVMQERKGGFVIPAEGTFELKPGGNHIMLMDLKSPLRTGTTVELTLVFSNG
                     HTSKTTAEVKPYAGAQETYQPGSSATTSGM"
     gene            complement(1927..2511)
                     /locus_tag="VHM65_04110"
     CDS             complement(1927..2511)
                     /locus_tag="VHM65_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704919.1"
                     /translation="MTSPGSGATRALRSGVVAVSIVGLACAAHVQGGGALPSGPGLAV
                     LIALTACVVGVTTGRRLGVYAIGATLGLGQVCLHVALGALTSGAATTAHVMVQGRAVA
                     HLPAAHVAHAGHSSGRMLLTHALATALTAILLAFGERALWALWERVCPRAAFAPIRLR
                     LGIARPAFAPGEVCRPASPSFRWPPLRGPPPVSV"
BASE COUNT          369 a          882 c         1001 g          332 t
ORIGIN      
        1 ctcaccgtcg gcagcgccgg ggaggatggc gaacacggcc gatcctacgg gagtcgtcca
       61 catgttgagc aggtccttcg cggccagccg gcgctggacg gggacgaact gctcggtgat
      121 gtcgcgctgg aaggtgatga acaccagccc cgagtcctcg ctgggcgctc cggtcgcccc
      181 ctcgtcgtag ttgtagggcc gccgcaggaa ccgctcccgg ggcaggtccg cggcggcgtg
      241 acggatatgg gcgaagtccg gtatgacgga cagccccgtc gccgacgtcg ccccgaggtc
      301 cgcggggtcc gtctcacgcg tgccggtcaa cggagcaccg ctggacaggc gccgcccgat
      361 gacctcctcc ttggccggcg ggtcgagcag gtcccaggcc gccaggttca tccggatccg
      421 gcgcacgaca aagccggtgc caccgcgcag ccaggacggc ccttcgtcgc cgacccagac
      481 gaggtcgtcg aactcgggcg tcgcgggctt ggggttgacg gtcccgtcga tctggcccat
      541 gagggtgcgc atcgaggcgg tcgacccagc cggccggcgg aagccgcgct gcacccagcg
      601 ctggcgggcc aggccaacgg cgtcgagcag gagtgaacgc acggcatggg cgagggtgat
      661 cgggtcgtcg ccgcacacct ggagcaggac atcgcccccg gtgtaggccg ggtccggcct
      721 gtcgaccgag aaggccggca gcggggccag ccaggccggg ggggcctcgg tcgggcgtgc
      781 cgcggcgacg gcctgggggc cgagcccgac cgtcacggtg agccgcgccg gcggaccgga
      841 caggtccggg gctgtgtcct gaagcgtggg ctggccctgg gtcagccgtg ccgcgtcatg
      901 ggtccacagg cgcaggaggc gacgcagctc gtcacgtccc gccccgggca gcaggtccag
      961 gccgacaagg acgaggttgg tctggggcgt ggtcgcgatc ccgctctggt ggggtccgta
     1021 gaacggctcg gccgcgatgg ccggcgccgc cgccggtgcg ggcgttgcgg ccgagccggt
     1081 cgcgcccgtc gaggtggtgt cgctgctcga acaccccgcg agagcacaga gcccggcgcc
     1141 gcctccgagg gcggcgccga acccgagtac cgcacgccga ctcggccgac ctgggtcgac
     1201 cgcgtccgcg cgcacctggt cagacatggc gcagggtcac atcccggacg tggtcgcgga
     1261 gctgcccggc tggtaggtct cctgggcgcc ggcatacggc ttgacctcag cggtggtctt
     1321 cgaggtgtgc ccgttggaga agaccagcgt cagctccacc gtggtcccgg tcctgagcgg
     1381 gctcttgaga tccatgagca tgatgtggtt gccacccggc ttgagctcga aggtgccctc
     1441 cgcggggatc acgaagccac ccttcctctc ctgcatcacc atcgtgtcac cgctcttgac
     1501 ggtctcgtgc agctggacca tgccggcccc ggcgaactcg gccttgacga gggtcacctc
     1561 ctcgtggctc tcgttgacga tcatgccgaa cgctgcggtc atgccgctgt cggcggcctt
     1621 gacccaggcg ctggtgaggc cgatctcgcg ctcggtgccg gccatcgagg tggtcgacga
     1681 ggagccggag acgggggagg tggcaccggc cgaggtcgtg ccgctcccgc atgcggtgaa
     1741 ggcggcgagg acggtgggga caagcgcggc ggccacgagg gctcgccgcg agggggagat
     1801 gctgttcatg tcggttcttc ctgtcagttc gagaccgcac cggcacaggg gtgggcccaa
     1861 tgtctatgag tggtgtgcgc tgggcgcgac aagatcgcgc ccggtcgagc gggcagtggc
     1921 ggtggctcag acggagacag gcggggggcc gcgcagtggc ggccaccgga aggacggcga
     1981 cgcgggacgg catacctcgc ccggggcgaa cgcgggacgg gcgatgccaa ggcgcaggcg
     2041 aatgggcgca aaggcggcac gcggacacac ccgctcccac agggcccaga gcgctcgctc
     2101 gccgaacgcg aggagtatgg cggtcagcgc ggtcgcgagc gcgtgggtga ggagcatccg
     2161 ccccgagctg tgcccggcgt gggccacatg ggcggccggg agatgggcca cggctcgccc
     2221 ctgcaccatc acgtgggccg tggtcgcagc gccagaggtg agggcgccga gggcgacgtg
     2281 cagacacacc tggccgagac cgagcgtcgc gccgatcgcg tagaccccca ggcgccgccc
     2341 ggtggtgacc ccgacgacgc acgcggtcag cgcgatgagg acggccaggc cgggaccgga
     2401 ggggagggcg ccgccgccct gcacgtgggc cgcgcacgcg agccccacga tgctcacggc
     2461 gaccacgccg gaacgcagcg ccctcgtggc gccgctcccg ggactggtca tgccacacag
     2521 ggtacccacg caccgcggcg gggccagcgg ggttccggtc agcggcgccg tacccctcgg
     2581 ggtc
//
LOCUS       DATAIX010000277         2924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_44948, whole genome shotgun sequence.
ACCESSION   DATAIX010000277 DATAIX010000000
VERSION     DATAIX010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44948"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VHM65_04115"
     CDS             <1..1086
                     /locus_tag="VHM65_04115"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2704920.1"
                     /translation="VLNAGDYNQQHQNLQKYLVAGSGAPDIAAIDEGFIVQFRNQSDK
                     FVNLLDLPGAGDYQSKYLGWKWNESLSADGKTQIGLGTDVGGQAMCYRSDLFATAGLP
                     TERAAVSALWPTWDEFIKVGQKYVAGIKDPKKKFVDAASNIMNPVLSQQPVGYVSSDE
                     KVAMDGGPKVAWDVATKAITTGISANLSAYTPEWNAAFKNGAFAVLACPAWQLGYIQQ
                     QAPDTAGKWDIAAIPGGGGNWGGSFLTIPKQGKNQAEAWEFIKWAVEPAQQIAIFKKV
                     GNLPSQPVLYDDPALKDFKNAFFNNAPVGQIFPETARKLQPQYYGKNSGPIRVAVENV
                     LVEQQQGKISGDAAWTKAVAAAEKANK"
     gene            1207..2157
                     /locus_tag="VHM65_04120"
     CDS             1207..2157
                     /locus_tag="VHM65_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2704921.1"
                     /translation="MTAQPPAPPAARPRGLRGRIHRADAKWSPYLFIAPFFLIFTAFG
                     LFPLLYNGVVSLRTWQLDSPDRDGWAGLENYRALLTDSDWWNALVNTVGMFLLSSVPQ
                     LVLALLLAGVLNRKLRFPTLMRMGVLLPYVTPIAASTLVFSAFFARDYGIANYLLSVV
                     HVDPIDWRADKWSSWVAIATMVNWRWIGYNALIYLAAMQSVPRDLYEAASIDGAGPRR
                     QFWSITVPMIRPTLLFTVVLSTIGGMQLFTEPLLFDNNVSNATGGSSGQFQTVAELIY
                     KTGWIDLDLGYAAAMSWAVFMVIIILAGLNSWLTNRVGRR"
     gene            2154..>2924
                     /locus_tag="VHM65_04125"
     CDS             2154..>2924
                     /locus_tag="VHM65_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2704922.1"
                     /translation="MSTPARVVNAPQAPRQGRRRPVVQRAPHDTPSGPLTYVALVLTM
                     LLSAFPLYWAFVVGSSTDEELSKLPPSVVPGNNLGSNVRQVFTQDNVYFVKSLANSVI
                     VTLTVTASVLFFCSLAGFAFAKLRFKGRNVLFVALILTMTVPNQLGIVALYILMSKFG
                     WNGTLPAVIVPGLVSAFGVFFMRQFALDSVPDELIESGRLDGASTFRIFWSIALPSMR
                     PALSILGLLTFVATWNDFQWPLVTLGGSENPTVQVALSN"
BASE COUNT          483 a         1056 c          904 g          481 t
ORIGIN      
        1 gtgctcaacg cgggtgacta caaccagcag caccagaacc tgcagaagta cctcgtcgcg
       61 ggctctggcg cccccgacat cgcggccatc gacgaaggct tcatcgtcca gttccgcaac
      121 cagtccgaca agttcgtcaa cctgctcgat ctgccgggtg cgggcgacta ccagagcaag
      181 tacctcggct ggaagtggaa cgagtccctc tcggcggacg gcaagacgca gatcggcctc
      241 gggaccgatg tgggtggcca ggccatgtgc taccggagcg acctgttcgc cacggccggc
      301 ctgcccaccg agcgtgccgc cgtctcggcg ctgtggccca cctgggacga gttcatcaag
      361 gtcggtcaga agtacgtcgc cgggatcaag gacccgaaga agaagttcgt cgacgcggcg
      421 agcaacatca tgaacccggt cctgtcgcag cagcccgtcg gctacgtgag cagcgacgag
      481 aaggtcgcca tggacggtgg cccgaaggtc gcctgggacg tggcgacgaa ggccatcacg
      541 accgggatct cggcgaacct gtccgcctac accccggagt ggaacgccgc cttcaagaac
      601 ggcgcgttcg ccgtcctcgc gtgcccggcc tggcagctcg gctacatcca gcagcaggcg
      661 ccggacaccg cgggcaagtg ggacatcgct gccatccccg gtggcggcgg caactggggc
      721 gggtcgttcc tgaccatccc caagcagggc aagaaccagg ccgaggcgtg ggagttcatc
      781 aagtgggccg tcgagcccgc gcagcagatc gccatcttca agaaggtcgg caacctgccc
      841 tcacagcccg tgctgtatga cgacccggct ctgaaggact tcaagaacgc gttcttcaac
      901 aacgcccccg tcggccagat cttccccgag acggcgcgca agctccagcc gcagtactac
      961 ggaaagaaca gcggcccgat ccgcgtcgcg gtcgagaacg tgctcgtcga gcagcagcag
     1021 ggcaagatct ctggtgacgc cgcctggacc aaggcggtcg ccgcagcgga gaaggccaac
     1081 aagtaggtca caagcgtcac ggtgggcgcg gcgactccag gtcccgcgcc caccgccgcc
     1141 gttggcgggg gtccgcgggg attgctgcgg accccctcaa cgagaaccag gactggaatg
     1201 acgagtatga cggcacagcc cccagcaccc cccgcggctc ggccccgagg tctgcgcggc
     1261 cggatccacc gcgcggacgc gaagtggtcg ccatacctgt tcatcgcgcc gttcttcctc
     1321 atcttcaccg ccttcgggtt gtttcccctc ctctacaacg gcgtcgtctc gctgcggacg
     1381 tggcagctcg actcgcccga ccgggacggc tgggccgggc tcgagaacta ccgggccctg
     1441 ctcaccgaca gcgactggtg gaacgcgctc gtcaacacgg tcgggatgtt cctcctctcc
     1501 agcgtgcccc agctcgtcct cgcgctcctg ctcgccgggg tcctcaaccg caagctgcgc
     1561 ttcccgaccc tcatgcggat gggggtcctg ctgccctacg tcaccccgat cgccgcgtcg
     1621 accctcgtct tcagcgcctt cttcgcccgg gactacggga tcgccaacta cctgctgtcc
     1681 gtcgtccacg tcgacccgat cgactggcgc gccgacaagt ggtcgtcgtg ggtcgccatc
     1741 gccacgatgg tcaactggcg ctggatcggg tacaacgccc tcatctacct cgccgcgatg
     1801 cagtcggtcc cgcgagacct ctacgaggcc gcgtcgatcg acggagcggg cccgcggcgg
     1861 cagttctgga gcatcaccgt cccgatgatc cggccgacgc tgctcttcac cgtcgtgctg
     1921 tcgacgatcg gcggcatgca gctgttcacc gaacccctgc tcttcgacaa caacgtgagc
     1981 aacgcgaccg gcggctcgag cgggcagttc cagacggtcg ccgagctcat ctacaagacc
     2041 ggctggatcg acctcgacct cggctacgcc gccgcgatgt cgtgggcggt cttcatggtc
     2101 atcatcatcc tggccggtct caactcctgg ctgacgaacc gggtggggcg acgatgagca
     2161 cacctgcccg ggtcgtgaac gcaccgcagg caccccgcca ggggcgccgc cgcccggtcg
     2221 tgcagcgcgc accgcacgac accccgtcag gcccgctcac ctacgtcgcc ctcgtgctga
     2281 cgatgctcct gtcggccttc ccgctgtact gggccttcgt cgtcgggtcg agcaccgacg
     2341 aggagctctc caagctgcca ccgagcgtcg tgcccggcaa caacctgggc agcaacgtcc
     2401 gtcaggtgtt cacgcaggac aacgtctact tcgtcaagtc cctcgccaac agcgtcatcg
     2461 tcaccctcac ggtgaccgcc tcggtcctgt tcttctgctc gctcgccggg ttcgccttcg
     2521 ccaagctgag gttcaagggc cgcaacgtgc tgttcgtggc gctcatcctc acgatgacgg
     2581 tgccgaacca gctcgggatc gtcgcgctct acatcctcat gtccaagttc gggtggaacg
     2641 gcacgctgcc cgcggtcatc gtccccgggc tggtgagcgc cttcggcgtg ttcttcatgc
     2701 gccagttcgc cctcgactcc gtcccggacg agctcatcga gtcgggccgg ctcgacggag
     2761 cctcgacctt ccggatcttc tggagcatcg ccctgccgtc gatgcgcccg gccctgagca
     2821 tcctcggcct gctcaccttc gtggcgacgt ggaacgactt ccagtggccg ctggtcaccc
     2881 tcggcgggag cgagaacccg accgtccagg tcgccctgag caat
//
LOCUS       DATAIX010000278         5050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_104382, whole genome shotgun sequence.
ACCESSION   DATAIX010000278 DATAIX010000000
VERSION     DATAIX010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104382"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..740)
                     /locus_tag="VHM65_04130"
     CDS             complement(186..740)
                     /locus_tag="VHM65_04130"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEX2704923.1"
                     /translation="MDRELWRVMRTSGPHVLPWGLTRRYGPLTQMRWDPHPAPRGEHV
                     DVGVLYAALDITTTLAEAFAATRCVETTAGNPHLVSWVPTRPLRLLDLTGGWALRNRA
                     AHSLAGATKDTCRSWAREICTAFADLDGLCAVSTMTGHPSIVLFARAEDSIPDSPATS
                     RPLTDPTVLLVLSVAAAEIGYSLL"
     gene            complement(812..1405)
                     /locus_tag="VHM65_04135"
     CDS             complement(812..1405)
                     /locus_tag="VHM65_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2704924.1"
                     /translation="MGSTVREALVTLGSPLTEDDVVAVLIESAERSARPGAAVLPSRE
                     REHLLADSGLSPAGRVALQGLLDSPGAAHSMIEAGRLANRVRLLADTLSAAEVADLLG
                     ITAGNVSRGASEGRLYSVHVGRGRRFPRWQFAGGQTLPGMAAIVAAVPRDLHPEAVHA
                     AMTRANEALGGMSPVDWLAGGGSPDVVGELVSDLGRW"
     gene            complement(1530..2966)
                     /locus_tag="VHM65_04140"
     CDS             complement(1530..2966)
                     /locus_tag="VHM65_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704925.1"
                     /translation="MSERRRQAQDPRSLRYDAPLLDAYLTFCAAHARPFTIPGHKGRS
                     DLVGSVVAGDVPYYAGLDTMKVERGVLATAQSRAAALWGADLARFSTGGSTHGNQALA
                     LALASPGDEVVVQRTVHRSVFLGLVLAGLDPVWVSPQVDAGTGVPLGVPAEVVRAALA
                     AHPSARAVLLGDPSYVGTTGDVAGAAAAAHAAGVPLVVDAAWAAHFGFHPGLPAHAIA
                     LGADAMVTSAHKMLPAYSQAALVLTRGPRIDRARLDAAVEATATTSPAGAILASIDAA
                     RALLARDGAQLLGRLLPLVAEARERLGAVPGVRVLHGPRVDPAKLVVLLAGSGADGVA
                     VERDLIAAGMPVELADRDLLIAIVTMADTAATVSELVERLVSSIEHHRGAPRPTVPAA
                     VWSVAPEQVVSPREAFFAPKVALTIRDAVGRVSGELVAPYPPGVPVLAPGERVTGESV
                     DALLTAREAGTRIAYAADPTLATLLVLG"
     gene            3125..4849
                     /locus_tag="VHM65_04145"
     CDS             3125..4849
                     /locus_tag="VHM65_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704926.1"
                     /translation="MTQTVQGSSSLSGLAAAVTALRDEVAAATLPLALPEAKTAMIAR
                     DRLLAQLDDYIIPRMTSLDAPILAVVGGSTGAGKSTLVNSLVGSVVSLPGVLRPTTRA
                     PVLVHHPDDARWFTGPRILPSLARVTGPAPQEAPQDPSAVRLVAIDTISPGLALLDAP
                     DIDSVVLANRALATALLAAADLWVFVTTAARYADAVPWELLTEAGERGTSVAIVLDRV
                     PPAALEEVRAHLGAMLRRQGLRSAPVFTILESRLDPDGLLPTTQTARLHGWLTDVADD
                     AQARSQIVHATLSGALESLDGRTRDLSRASRSQLEATATLAAEVDAAYADALGRVRHG
                     ISDGSLLRGEVLARWQEYVGTGELVRQVESTVSRVRDRLTSLVTGDPAPTQGLGEALH
                     SGAADLIVAHAQVAASTAARSWRSAAGGPALLAGHPELAQSSAGLPEAVARMVRDWQG
                     EILDLVRGEGKSRRATARMMAYGVNGVGVVLILVTFAHTGGITGAEAGIAGGTAVLGQ
                     KLLEAIFGDQAVRELAARARRLLVAKVEALYAAEGQRYHDVVAALEVSGDQPRRLDEA
                     ERTLRSAR"
     gene            4846..>5050
                     /locus_tag="VHM65_04150"
     CDS             4846..>5050
                     /locus_tag="VHM65_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704927.1"
                     /translation="MSPLVMGSRRVTAVSADTLADQTEALVQALDAAGACLPGDAVSA
                     ARGVATKVAERMTMTGDHTVVALA"
BASE COUNT          681 a         1810 c         1913 g          646 t
ORIGIN      
        1 ccgctgagcc tcccacccgg gccgggtcgg cgcggggtgg ccagggcctg gacgaacgcg
       61 caggccgctg cgcccagggc cgcgaccgcc tggcccgtag ggagtcgggt cgagggtggc
      121 cagaacgtca ggagaaggcg ccgcgccggg tcggctgaag cgagccgcca acggccacag
      181 cgcacctaca gcagcgagta gccgatctcg gccgcggcca ctgacaggac gagcaggacg
      241 gttgggtcgg tgagcggccg acttgtggcc gggctgtccg gtatggagtc ttccgcccgc
      301 gcgaaaagga cgatgctcgg gtgaccggtc atcgtcgaca ccgcgcacag gccgtcgagg
      361 tccgcgaagg ccgtgcagat ctcgcgagcc cagctccggc aggtgtcctt ggttgccccc
      421 gccagcgagt gagcggccct gttgcgcagc gcccagcccc ccgtcaggtc gagcaggcgg
      481 agcggccgag tgggcaccca cgagaccagg tgcgggttgc ctgccgtggt ctccacgcat
      541 cgggtcgcgg cgaacgcctc ggcgagcgtc gtcgtgatgt cgagcgcggc atacaggact
      601 ccgacgtcga cgtgctcccc tctcggggca ggatgggggt cccaccgcat ctgcgtcaac
      661 ggcccgtacc ttcgggtgag gccccagggg aggacgtgcg gaccgctcgt gcgcatcacc
      721 cgccagagct cgcggtccat ctcgatgaca ttccggggct ctcgcagcag tggctccggt
      781 ggtcgtctgg ggaccttggg aaccggcccc ctcaccatcg gccgaggtcg ctcaccagct
      841 cgcccaccac gtcggggcta ccgccaccgg cgagccagtc gaccggcgac atcccgccaa
      901 gggcctcgtt ggccctggtc atggccgcgt ggaccgcctc cggatggagg tctcgcggga
      961 ccgctgcgac gatcgcagcc atgccgggca gagtctggcc gccagcgaac tgccagcgtg
     1021 ggaacctgcg gccgcgcccg acgtggacgc tgtagaggcg accctcggac gcgccacggg
     1081 agacgttgcc cgccgtgatc cccaacaggt ccgcaacctc ggcagcgctc agggtgtcgg
     1141 ccaggagccg caccctgttg gccagccgac ccgcttcgat catggagtgc gccgcgccag
     1201 ggctgtcgag cagcccctgg agcgccacgc ggccggctgg ggacaggccg ctgtcggcga
     1261 gcaggtgctc acgctcccga ctgggcagga cggcagcacc agggcgcgcc gaccgctcgg
     1321 ccgactcgat gaggacagcg accacgtcat cttcggtcag aggcgacccg agggtgacaa
     1381 gtgcctctcg cacggtgctg cccatgctct gatcctagcg caattagacg caacagactg
     1441 cgtctgtttg cgagctgggt cgtgccagtg ctcggtcgtg ccagtggggg tcagccgccc
     1501 ggagcgcggg tcagcggccc gtcgagtggc tagcccagga ccaggagcgt cgcgagggtg
     1561 gggtcggcgg catacgcgat cctcgtcccg gcctcgcgcg cggtgagcag ggcgtcgacg
     1621 gactcgccgg tgacccgctc gccgggtgcc aggacgggaa caccgggcgg gtatggcgcg
     1681 acgagctcgc cgctgacccg cccgaccgcg tcgcggatgg tgagggcgac cttgggcgcg
     1741 aagaacgcct cgcggggcga gacgacctgc tccggggcga ccgaccacac ggctgcgggg
     1801 acggtcggcc gaggggcgcc gcggtggtgc tcgatcgagg acacgaggcg ctcgacgagc
     1861 tcgctcacgg tcgccgcggt gtcggccatc gtcacgatcg cgatgagcag gtcgcggtcg
     1921 gccagctcga ccggcatacc ggcggcgatg aggtcgcgtt cgacggcgac cccgtcggca
     1981 cccgaaccgg cgagcaggac gacgagcttg gcggggtcga cccggggacc gtgcaggacc
     2041 cggacaccgg gcactgcccc gagccgctcg cgagcctcgg cgaccagggg gagcaggcgg
     2101 ccgagcagct gggcgccgtc gcgggccagc agcgcccggg ccgcgtcgat gctcgcgagg
     2161 atcgcgccgg cgggactcgt ggtcgctgtc gcctcgacgg ccgcgtcgag ccgggcccgg
     2221 tcgatgcggg ggccccgggt caggacgagc gcggcttggg agtaggccgg gagcatcttg
     2281 tgcgcgctcg tcaccatggc gtcggcgccg agggcgatcg catgggcggg caggccgggg
     2341 tggaagccga agtgggcggc ccaggcagcg tccacgacca gggggacccc cgcggcgtga
     2401 gcggccgcgg ccgcgcccgc gacatcgccg gtcgtcccga cgtaggaggg gtcgcccagg
     2461 aggaccgcgc gggccgaagg gtgggccgcg agcgcggcgc ggacgacctc ggcgggcaca
     2521 ccgaggggca ccccggtgcc ggcgtcgacc tgcggcgaca cccagacggg gtccagcccg
     2581 gccaggacga gcccgaggaa caccgagcgg tggacggtgc gctggacgac gacctcgtcg
     2641 cccgggctgg ccagggcgag ggccagggcc tggttgccgt gggtcgaccc gccggtcgag
     2701 aagcgggcca ggtcggcgcc ccacagcgcg gccgcccggg actgggccgt cgccaggaca
     2761 ccccgctcga ccttcatggt gtccaggccg gcgtagtagg gcacgtcacc ggccacgacc
     2821 gacccgacca ggtcggagcg ccccttgtgc cctgggatcg tgaacggccg ggcgtgggcg
     2881 gcgcagaagg tgaggtacgc gtcgagcagg ggcgcgtcat accgcaggct tctggggtcc
     2941 tgggcctgcc gtcgccgctc gctcacgggc gtcacgctac cgtcggccgt gcggcccggc
     3001 ggtcccaggc tttggccggt cagtcgttat cctgaggcag gcccgtgtcg cggacgtccc
     3061 acaccgatcc gcgccacgag ccgcacgacg gccgacgcca ccgactctag ggaagttgct
     3121 cagcgtgacc cagaccgtgc agggcagcag cagcctgtcc ggcctcgcgg ccgcagtgac
     3181 cgcgctgcgc gacgaggtgg cggcggcgac cctgccgctc gcgcttcccg aggccaagac
     3241 cgccatgatc gcgcgggacc ggctgctggc ccagctcgac gactacatca tcccgaggat
     3301 gacctcgctc gacgcgccga tcctcgccgt ggtcggaggg tccaccgggg ccggcaagtc
     3361 gacgctggtc aactccctcg tcggttcggt cgtgagcctg cccggagtcc tgcggcccac
     3421 gacccgggct ccggtcctcg tgcaccatcc cgacgacgcc cgctggttca ccggaccgcg
     3481 gatcctgccc tccctggccc gggtcaccgg tccggcaccc caggaggcac cgcaagaccc
     3541 gtccgccgtg cggctcgtgg cgatcgacac catctcgccg ggcctggccc tgctcgacgc
     3601 cccggatatc gactcggtcg tcctggccaa ccgcgccctc gcgacggcgc tgctcgccgc
     3661 cgccgacctg tgggtgttcg tcaccaccgc ggcccggtat gccgacgccg tcccgtggga
     3721 gctgctgacc gaagcaggtg agcggggtac gtcggtggcg atcgtgctgg accgcgtccc
     3781 gccggccgcg ctcgaggagg tccgggctca tctgggcgcg atgctgcgcc ggcaagggct
     3841 gcggagcgcc ccggtcttca ccatcctgga gagtcgcctc gaccccgacg ggctgctgcc
     3901 cacgacgcag acggcgcgcc tgcatggctg gctcaccgac gtcgccgacg acgcccaggc
     3961 ccggtcgcag atcgtccacg cgacgctctc cggggcactg gagtccctcg acggccgcac
     4021 gcgtgacctg tcccgcgcgt cgcgttccca gctggaggcg accgcgacgc tcgcggccga
     4081 ggtggacgcg gcatacgccg atgcgctcgg tcgggtccgc catggcatca gcgacggatc
     4141 gctgctgcgc ggcgaggtgc tcgctcgctg gcaggagtat gtcgggaccg gtgagctcgt
     4201 ccgccaggtc gagtcgaccg tctcgcgggt ccgggaccgg ctgacctcgc tggtgacggg
     4261 tgaccccgcg cctacccagg gtctcggcga ggcgctgcac tcgggtgcgg ccgacctcat
     4321 cgtcgcgcac gcccaggtgg ccgcgtcgac ggccgcccgc tcctggcgga gtgcggcggg
     4381 tggcccggcg ttgctcgccg gtcaccccga gctggcgcag tcctcggcgg gcctgcccga
     4441 ggccgtggcc cggatggtgc gcgactggca gggcgagatc ctcgacctcg tccgcggcga
     4501 gggcaagagc cggcgcgcca cggcgaggat gatggcgtat ggcgtcaacg gggtgggggt
     4561 cgtgctcatc ctcgtcacct tcgcgcacac cggcggcatc accggggccg aggctggaat
     4621 cgcaggcggc acagcggttc tcgggcagaa gctgctcgag gcgatcttcg gcgaccaggc
     4681 cgtgcgcgag ctcgcggcga gggcccggag gctgctcgtc gccaaggtcg aggccctgta
     4741 tgccgccgag ggtcagcgct atcacgacgt ggtcgctgcg ctcgaggtct ccggggacca
     4801 gccgcgccgc ctcgacgagg ccgagcggac gctgaggtca gcccggtgag cccgctcgtg
     4861 atggggtcgc gccgggtcac ggcggtgtcg gccgacaccc tggccgacca gaccgaggca
     4921 ctcgtgcaag cactcgacgc ggcgggtgca tgcctgcccg gcgacgccgt ttcagccgcc
     4981 cggggcgtgg ccacgaaggt ggccgagcgg atgacgatga ccggcgacca cacggtcgtc
     5041 gcgcttgccg
//
LOCUS       DATAIX010000279         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_244861, whole genome shotgun sequence.
ACCESSION   DATAIX010000279 DATAIX010000000
VERSION     DATAIX010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244861"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..735
                     /gene="grpE"
                     /locus_tag="VHM65_04155"
     CDS             73..735
                     /gene="grpE"
                     /locus_tag="VHM65_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX2704928.1"
                     /translation="MTHPNPVGPAHDVEVGPEQFGPTTHGGGTASAEPGGFDEFDHAP
                     TAPAQDAVADVGDDELNTEAVNDEASFRGLDTHPDTLLAAERLFDLQRLHAEYVNYKR
                     RVDRDRDVARDLGISQVLEAMLPVLDDIHLAREHGDLEGGPLSAIAEKVEHTLAKFGV
                     ERFGRVGDEFDPTVHEALMHVEAELPPEATTTTVVHVLQPGYRAGDRLVRPARVSVAD
                     PQ"
     gene            757..1776
                     /locus_tag="VHM65_04160"
     CDS             757..1776
                     /locus_tag="VHM65_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX2704929.1"
                     /translation="MASQDWFEKDFYAILGVPQDADAATVKKTYRKLARKLHPDQNAN
                     DPKSEERFKEIGEAYSVLADPEQRKQYDAVRAMSRGGARFTAGGQPGGAGFEDLFSNM
                     FGGGGGGGNTRFTTSGGRGADPDLQDLLGGLFGGAGGTSYPGGFQPGFGAPRGPRRGS
                     DVEARATLTFRDAVQGSTVTLSTASGERITTRIPAGVKDGQKIRLRGKGSPGDSGAPA
                     GDLMLTVTVTQHPVFGRDGNNLTVDLPVTFAEAALGATVTVPTLDGETVKVKVAPGTP
                     SGRVLRVRGRGVKHGDTPGDLLAKVQVVVPQRLTDEARAAVEALAAQESGQDPRAELI
                     AKAGS"
     gene            1778..2242
                     /locus_tag="VHM65_04165"
     CDS             1778..2242
                     /locus_tag="VHM65_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2704930.1"
                     /translation="MAPRHSPLGPGMPEEGAPVFVISVAAQLAGMHAQTLRQYDRLGL
                     VSPSRTSGGGRRYSARDVALLRQVQRLSQEEGVSLAGIQRILELENHVEALRDRVTEL
                     TEHLDAAQAMLRSGARVFAAGPRGDIVAVRPGQRTVRPGGASRSLVVWRPQR"
     gene            complement(2342..>2719)
                     /locus_tag="VHM65_04170"
     CDS             complement(2342..>2719)
                     /locus_tag="VHM65_04170"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEX2704931.1"
                     /translation="LKYDVGFWRPVQAVAGADADGNPATIPEPGWTSLLPTPPYPEYV
                     SGHASLTGSGAEVIRRMLGDDTPLTLHSSVTQTDRSYPTLSSIEADALNSRIWGGLHF
                     RAAMDDGYRLAHRTAVRVLEHLV"
BASE COUNT          402 a          978 c          970 g          369 t
ORIGIN      
        1 cggctcgggc gccggctcgg acgacgacgt ggtcgacgcc gaggttgtcg aggacgcgcc
       61 caaggacgcc aagtgaccca tccgaacccg gtcgggccgg cccacgacgt ggaggtgggg
      121 ccggagcagt tcggtcccac aacccacggc gggggtacgg cttcggccga gccgggcggg
      181 ttcgacgagt tcgaccacgc gccgacggcc ccggcgcagg acgcggtcgc cgatgtcggc
      241 gacgacgagc tcaacaccga ggccgtcaac gacgaggcct cgttccgcgg cctcgacacg
      301 catccggaca ccctgctcgc tgccgagcgg ctcttcgacc tgcagcggct gcacgccgag
      361 tacgtcaact acaagcgccg cgtcgaccgt gaccgtgacg tcgcccgtga cctcgggatc
      421 tcccaggtcc tcgaggcgat gctcccggtc ctcgacgaca tccatctcgc ccgcgagcac
      481 ggcgacctcg agggcggccc gctctcggcg atcgccgaga aggtcgagca cacgctggcg
      541 aagttcggtg tcgagcgctt cgggcgggtc ggcgacgagt tcgaccccac cgtccacgag
      601 gcactgatgc acgtcgaggc cgagctccca cccgaggcca cgacgacgac cgtcgtccac
      661 gtgctccagc ccggataccg ggcaggggac cgtctggtcc gcccggcccg cgtctcggtc
      721 gccgaccccc agtaacagag aggaggcgcg ccagggatgg caagccaaga ctggttcgag
      781 aaggacttct acgcgatcct cggcgtgccc caggatgccg atgccgcgac ggtcaagaag
      841 acctatcgca agctggcacg caagctgcac cccgaccaga acgccaacga ccccaagtcc
      901 gaggagcgct tcaaggagat cggcgaggcc tactcggtcc tcgccgaccc cgagcagcgc
      961 aagcagtatg acgccgtccg cgccatgtcc cgtggcggcg cccggttcac cgccggcggc
     1021 cagccgggcg gcgccggctt cgaggacctc ttctcgaaca tgttcggcgg cggcggcggt
     1081 ggtggcaaca cccgcttcac cacctccgga ggccgcggcg ccgaccccga cctgcaggac
     1141 ctgctcgggg gactgttcgg cggcgctggt ggcacgtcat accccggggg tttccagcct
     1201 ggcttcggcg cgccccgcgg accgcggcgc ggcagtgatg tcgaagctcg cgcgacgctg
     1261 acgttccgcg atgccgtcca gggctcgacg gtcaccctga gcaccgcttc gggggagcgc
     1321 atcaccacgc gaatcccggc cggtgtcaag gatggtcaga agatccggct gcgcggcaag
     1381 ggctccccgg gcgactcggg tgcaccggcg ggagacctca tgctgacggt cacggtgacc
     1441 cagcaccccg tgttcggccg agacggcaat aacctcaccg tcgacctgcc ggtcaccttc
     1501 gccgaggcgg ctctcggcgc cacggtcacg gtgccgaccc tcgacggcga gacggtcaag
     1561 gtcaaggtgg cgcccggaac cccgagcggc cgggtgctgc gtgtgcgcgg ccgcggtgtc
     1621 aagcatggcg acacgcccgg cgacctgctc gccaaggtcc aggtcgtcgt gccgcagcgg
     1681 ctcaccgacg aggcgcgcgc ggccgtcgag gcactcgcgg cccaggagtc cggtcaggac
     1741 ccgcgcgctg agctcatcgc caaggcgggc agctgagatg gcacctcgcc actcacccct
     1801 tgggcccggt atgccggagg agggcgcccc ggtgttcgtc atctcggtcg ccgcgcagct
     1861 cgccggcatg cacgcccaga cgctgcgcca gtacgaccgg ctcggtctcg tctcgccgtc
     1921 gcggacgtcc ggtggaggcc ggcgctactc ggcccgtgac gtcgctctgc ttcgtcaagt
     1981 acagcggctc tcccaggagg agggtgtgtc cctggccggg atccagcgga tcctcgagct
     2041 ggagaaccac gtcgaggccc tgcgggaccg ggtgaccgag ctgactgagc acctcgacgc
     2101 ggctcaggcg atgctccggt cgggggcacg ggtctttgcc gcaggtcccc gcggcgacat
     2161 cgtcgcggtc cgtccgggcc agcgcacggt gcggccgggt ggggcctccc gatccctcgt
     2221 cgtctggcgc ccgcagcgct gacgcccccg ccccgacgag gtcaggtcac cgaggcgggt
     2281 gaccatgatg atgacctccc ggggccaggc gcctggtgtg tcgtgtcggc gagtccgtcg
     2341 gtcagacgag gtgctccaac acccttaccg cggtccgatg ggcgagccgg tagccgtcgt
     2401 ccatggcggc ccggaagtgc agcccgcccc agatgcggga gttgagtgcg tcggcctcga
     2461 tgctcgacag ggtcgggtag gaacggtcgg tctgggtgac ggacgagtgc agcgtcagcg
     2521 gggtgtcgtc gccgagcatc cgccggatca cctccgcccc cgatccggtg agggaggcgt
     2581 ggccgctcac gtactccggg tacggcgggg tgggcagcag cgaggtccag cccggctcgg
     2641 ggatggtcgc cgggttgccg tcggcgtcgg cacccgccac cgcctggacc ggacgccaga
     2701 agcccacgtc gtacttgag
//
LOCUS       DATAIX010000280         2584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_164670, whole genome shotgun sequence.
ACCESSION   DATAIX010000280 DATAIX010000000
VERSION     DATAIX010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164670"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1232
                     /locus_tag="VHM65_04175"
     CDS             240..1232
                     /locus_tag="VHM65_04175"
                     /inference="COORDINATES: protein motif:HMM:NF019346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2704932.1"
                     /translation="MRSRPVAQASPLGAGDLARLREVASGIVATMSRVIEGKADVIET
                     AVTVLFAEGHLLIEDVPGVGKTMLAKALARSIDCTVRRIQFTPDLLPSDITGVSVFNQ
                     DVRDFEFRPGAVFANVVVGDEINRASPKTQSALLECMEEGQVTVDGHTYVLHRPFLVL
                     ATQNPIEMEGTYPLPEAQRDRFMARLSMGYPSNAAELVMLDTHGGAPAIDELTPSTDG
                     ATVARMIEVTRGIYASPALRQYIVDIATATRGTSALRLGASPRASLHLLRASRAQAAL
                     RGRDHVLPDDVQRIVGPVLTHRLLLSSESQMARRSASDVLRDIMSRLPVPAGPR"
     gene            1255..2511
                     /locus_tag="VHM65_04180"
     CDS             1255..2511
                     /locus_tag="VHM65_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX2704933.1"
                     /translation="MTVRDALTTRGWAFASAGGALLLAGLGLGLRDLTRIGVLLLALP
                     LISGILARRHNLDVEVERTLRPMRVSVGDVATVRMEIVNRGRLSTPLLIAEERLDHVL
                     GDRPRFLIARTRPGQRRRVDYQVRSHLRGRHQLGPVVVQVRDPFGMSLRGSAVGAPSR
                     IVVRPRVVPLGDRHPPGSGVGAEGEIPFMVALHGEDDVSIREFRDGDDLRRIHWPATA
                     HSGELMVRQEDRPARRRAIILLDPRESAHRGTGVSSSFESAVTATASIIDHLNNAGYA
                     VHLVAVETVQDERAADALDTDRALDLLAVVKTWPAAELGPLVRAAQTVGAAGGLLVAV
                     VAGGGEDELRTIASLRHPGNTALAMVIDPAGFQDGPPQLSAPRGADPADLFRHTGWSV
                     GVLHQGQSVAEAWAGVPPGRRGVFTG"
BASE COUNT          348 a          945 c          934 g          357 t
ORIGIN      
        1 gctggagcgg gttggtgcag gtcagcgccc tggcgcaggg gtggagtgag gtggaggggc
       61 gaggccaccc gaggcgcgct ggcgcgtcat accggcgctc ccgagggaag cgcccggtcc
      121 cctcggaggg ggctgagctc acgaaacggg ccgtggcacg ggcacgacat ggtgccggtg
      181 gagcgttgtg ggggaagatg ccgtgaccgg acaacgacaa ggacgcacac gtggacgaga
      241 tgagaagccg cccggtcgcc caggcgagcc cgctcggcgc gggcgacctg gccaggctgc
      301 gggaggtggc ctcggggatc gtcgcgacga tgtccagggt catcgagggc aaggccgacg
      361 tcatcgagac ggcggtcacc gtcctgttcg ccgaagggca cctgctcatc gaggacgtcc
      421 ccggcgtcgg caagacgatg ctcgccaagg cgctggcccg ctcgatcgac tgcaccgtgc
      481 ggcggatcca gttcacaccg gacctgctgc cgagcgacat caccggcgtg agcgtcttca
      541 accaggacgt gcgcgacttc gagttccgcc ccggcgcggt gttcgccaac gtcgtcgtcg
      601 gcgacgagat caaccgcgcg tcgcccaaga cccagtcggc cctgctcgag tgcatggagg
      661 aggggcaggt cacggtcgac gggcacacct acgtgctgca ccggccgttc ctcgtcctcg
      721 cgacgcagaa ccccatcgag atggagggga cctacccgct gcccgaggca cagcgggacc
      781 ggttcatggc ccgcctctcg atgggctacc cgtccaacgc cgccgagctg gtcatgctcg
      841 acacccacgg cggagctccg gcgatcgacg agctgacccc gtcgaccgac ggcgcgacgg
      901 tggcccggat gatcgaggtg acccgcggca tctacgcgag cccggcactt cgccagtaca
      961 tcgtcgacat cgcgacagcc acgcgcggga cgtccgcgct acgcctcggt gcgtcccccc
     1021 gggcgagcct gcacctgctg cgcgcatccc gggcgcaggc cgctctgcgc gggcgcgacc
     1081 acgtcctgcc ggacgacgtc cagcgcatcg tcgggccggt cctgacccat cggctgctgc
     1141 tctcctccga gagccagatg gcgcgccgct cggccagcga cgtgctgcgc gacatcatgt
     1201 cgcgcctgcc cgtcccggcc ggtccgcgat gagcccgggg caggaagccc ggggatgacg
     1261 gttcgcgacg cgctcacgac caggggctgg gccttcgcct cggcgggcgg cgcgctgcta
     1321 ctcgccggtc tcggcctcgg gctgcgcgac ctcacccgta tcggcgtcct gctcctcgcg
     1381 ctgcccctga tctccgggat cctcgcgcgc cggcacaacc tcgacgtcga ggtcgagcgc
     1441 actcttcgtc ccatgcgggt cagcgtcggc gatgtggcga ccgtgcgcat ggagatcgtc
     1501 aaccgcggac ggctctcgac gcccctgctc atcgccgagg agcgtctcga ccatgtcctc
     1561 ggtgaccggc cccggttcct catcgcccgc actcggccgg gccagcggcg ccgggtcgac
     1621 taccaggtgc gcagccacct gcgcgggcgg caccagctcg gcccggtcgt cgtccaggtg
     1681 cgggacccct tcgggatgtc gctgcgcggg agcgcggtgg gcgcgccgag ccggatcgtc
     1741 gtgcgtccgc gtgtcgtgcc gctcggcgac aggcacccgc ccggaagcgg cgtcggcgcc
     1801 gagggcgaga tcccgttcat ggtggcgctg cacggagagg acgacgtgtc catccgggag
     1861 ttccgcgacg gggacgacct gcgccggatc cactggcccg cgacggccca ctccggcgag
     1921 ctcatggtcc gtcaggagga ccgtccggcc cggcgccgcg cgatcatcct gctcgacccg
     1981 cgcgagagcg cccatcgcgg cacgggtgtc tcgagctcgt tcgagagcgc ggtgaccgcc
     2041 acggcctcga tcatcgacca tctcaacaac gccgggtatg ccgtccacct cgtcgccgtg
     2101 gagaccgtcc aggacgagcg cgcggccgac gcccttgaca ccgatcgggc actggacctc
     2161 ctggccgtcg tcaagacctg gcccgccgcc gagctcgggc cgctcgtgcg tgccgcgcag
     2221 acggtcgggg ccgcgggagg gctcctcgtg gcggtcgtgg ccggtggcgg tgaggacgag
     2281 ctgcggacga tcgcgtcgct gcggcatccg ggcaacaccg ccctggccat ggtcatcgac
     2341 ccggcgggct tccaggacgg tcccccgcag ctttcggcgc cgcgcggcgc cgacccagcc
     2401 gacctgttcc gtcataccgg gtggtcggtc ggggtcctgc accagggcca gtccgtcgcc
     2461 gaagcctggg ccggcgtgcc gcccggacgc cggggggtgt tcaccggatg aagggcgacg
     2521 gcgcagggtc ggccctggcc gcggtcgcga cgctcgcggc agcctttccc ctcaccacgc
     2581 tgtt
//
LOCUS       DATAIX010000281         2486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_45459, whole genome shotgun sequence.
ACCESSION   DATAIX010000281 DATAIX010000000
VERSION     DATAIX010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45459"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VHM65_04185"
     CDS             <1..238
                     /locus_tag="VHM65_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704934.1"
                     /translation="ATRWVQVRVPGATHQAKHTRGLVAREICRAGADPRSVPEPGAVL
                     SERFMVTLTGPARPGRPWVLEAVARSPRAGAAVS"
     gene            complement(333..1538)
                     /locus_tag="VHM65_04190"
     CDS             complement(333..1538)
                     /locus_tag="VHM65_04190"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF005870.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEX2704935.1"
                     /translation="MPDPLDPPDAPPALRPDTLAIRGGLTRSGFDETAEALFLTSGFV
                     YDSAEQAEAAFKDEIDRFIYSRYGNPTVAMFEERLRLLDGAQACFATASGMAAVFTAL
                     AALLGKGDRVVSSRALFGSCLVILDEILPRWGIETVFVDGTDLDQWREALAVPTTAVF
                     FETPSNPMQELVDIAAVSALAHAAGAKVVVDNVFGTPIHSRPLDHGADILVYSATKHI
                     DGQGRTLGGAILGPADFVTGPVKNLMRHTGPSMSPFNAWVLVKGLETMSLRVERQSLS
                     ALTLAQALEAHPRVRAVRYPWLPSHPQHELARAQMLGGGTVVTFEVDGGRPGAFRMLN
                     ALEIIDISNNLGDSKSLATHPATTTHRRLGPEGRAAAGITDGVIRISVGLEDVRDLVD
                     DLTRALDVR"
     gene            complement(1545..1958)
                     /locus_tag="VHM65_04195"
     CDS             complement(1545..1958)
                     /locus_tag="VHM65_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2704936.1"
                     /translation="MRYAGDVTSQQAYDALRDDPHAVLVDVRTRAEWAYVGLPVLDAV
                     GKDVVCVEWQTFPDGGLNPHFLDDLRAAGVGDEQAVYFICRSGARSAAAASALSVAGG
                     GPAYNIADGFEGPHDAHGHRSHSGWKVAGLPWRQG"
     gene            complement(1981..>2486)
                     /locus_tag="VHM65_04200"
     CDS             complement(1981..>2486)
                     /locus_tag="VHM65_04200"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX2704937.1"
                     /translation="AAAAAAVQRLVGGAGATVLTSSLARARQTGRHIADALGVEPVED
                     AEWDEQGFGDWDGASVTDLIRDHPDLLARFRNDPDYTRPGGETQTDLEARVIPALDRA
                     VARGGTVVIATHRKPILVVLRRVLHLPSESFWTLATAPASLTAIATWADGNVSVAFTN
                     RTDHLGG"
BASE COUNT          332 a          866 c          949 g          339 t
ORIGIN      
        1 cgccacgcgc tgggtgcagg tccgggtgcc gggagcgacg caccaggcca agcacacccg
       61 gggtctcgtg gcccgggaga tctgccgggc tggcgcggac ccgcggtcgg tgcccgagcc
      121 cggcgcggtc ctgtccgagc gcttcatggt gacgctcacc gggccggccc gccccggacg
      181 tccctgggtc ctcgaggccg tcgcccgcag cccgcgcgcc ggggcggcgg tgtcgtgatc
      241 tggccggacg cacggccctg gctggtgcca ttggcctcgt ggggtagccc ggcggcgagc
      301 cgcgccgcgc tggacgtcgg cgccgatcgt ggctaccgga cgtcgagggc acgggtgagg
      361 tcgtcgacga ggtcgcggac gtcctcgaga ccgaccgaga tgcggatgac gccgtcggtg
      421 atgccggccg cggcgcgccc ctcggggccg agccggcgat gggtcgtcgt ggccgggtgc
      481 gtggccaggg acttggagtc gccgaggtta ttcgagatgt caatgatctc aagggcattg
      541 agcatacgga acgccccggg ccgaccgccg tcgacctcga atgtcacgac cgtcccgcca
      601 ccgagcatct gcgcccgtgc gagctcatgc tggggatgtg acgggagcca ggggtagcgg
      661 accgcgcgaa cccgcgggtg ggcctcgagc gcctgggcga gcgtgagcgc actcagcgac
      721 tgccgctcga cccgcaggct catcgtctcc aggcccttga cgagcaccca ggcgttgaag
      781 ggtgacatcg acggcccggt gtggcgcatg aggttcttga ccggcccggt gacgaagtcg
      841 gccggcccga ggatggcccc gccgagggtg cgcccttgcc cgtcgatgtg cttggtcgcc
      901 gagtagacga ggatgtcggc gccgtggtcg agcgggcggg agtggatcgg cgtgccgaac
      961 acgttgtcca cgacgacctt ggcccccgcc gcgtgggcca gcgccgacac ggccgcgatg
     1021 tcgacgagct cctgcatcgg gttgctcggc gtctcgaaga acaccgccgt cgtcggcacc
     1081 gccagcgcct cccgccactg gtccaggtcg gtcccgtcga cgaacacggt ctcgatgccc
     1141 cagcgcggca ggatctcgtc gaggatgacc aggcacgagc cgaacagcgc gcgcgagctg
     1201 acgacccggt cgcccttacc gagcagggcc gcgagcgcgg tgaacacggc cgccataccg
     1261 gatgccgtgg cgaagcaggc ctgcgcgccg tcgagcaggc gcagccgctc ctcgaacatc
     1321 gcgaccgtcg ggttgccgta gcgcgagtag atgaaccggt cgatctcgtc cttgaaggcc
     1381 gcctcggcct gctcggcgct gtcgtagacg aacccgctcg tgaggaacag cgcctcggcc
     1441 gtctcgtcga aaccgctgcg ggtcagcccg ccccggatgg cgagggtgtc ggggcgcagc
     1501 gccggcggcg cgtcaggggg gtccagcggg tcgggcatgt cggctcagcc ctggcgccac
     1561 ggcagcccgg cgaccttcca gccggagtgg ctgcggtggc cgtgcgcgtc gtgcgggccc
     1621 tcgaacccgt ccgcgatgtt gtatgccggc ccgccgccgg ccacgctcag tgccgaggcg
     1681 gccgccgccg accgggcacc gctgcggcag atgaagtaga ccgcctgctc gtcgcccacg
     1741 cctgcggcgc gcaggtcgtc gaggaagtgc gggttgagcc cgccgtccgg gaacgtctgc
     1801 cactcgacgc agacgacgtc cttgccgacg gcgtcgagga cgggcagccc gacatacgcc
     1861 cactcggcgc gggtgcgcac gtcgacgagg accgcgtgcg ggtcgtcgcg gagggcgtcg
     1921 taggcctgct gactcgtgac atcaccggcg tacctcatgg gcccagggtc gcacgctcgc
     1981 tcagccgccg aggtggtccg tccggttcgt gaacgcgaca ctgacattgc catcggccca
     2041 ggtcgctatc gcggtgagcg acgccggtgc ggtcgccagg gtccagaagc tctccgacgg
     2101 caggtgcagg acgcggcgca ggacgacgag gatcggcttc cggtgggtcg cgatgacgac
     2161 ggttccgcct cgggcgacgg cccggtcgag ggccgggatg acccgcgcct cgaggtcggt
     2221 ctgcgtctcg ccgccgggcc gggtgtagtc ggggtcgttg cggaaacggg ccagcaggtc
     2281 agggtggtcg cggatgaggt cggtgaccga ggcaccgtcc cagtcaccga agccctgctc
     2341 gtcccactcg gcgtcctcga ccggctcgac gccgagggca tcggcgatgt gccggccggt
     2401 ctgacgggcc cgcgccagtg acgaggtgag gaccgtggca ccggcgccgc cgacgagccg
     2461 ctgcacggcc gcggccgcgg ccgcgg
//
LOCUS       DATAIX010000282         1723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_15310, whole genome shotgun sequence.
ACCESSION   DATAIX010000282 DATAIX010000000
VERSION     DATAIX010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15310"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="VHM65_04205"
     CDS             <1..1022
                     /locus_tag="VHM65_04205"
                     /inference="COORDINATES: protein motif:HMM:NF025309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HEX2704938.1"
                     /translation="AGQDDRCLDAQGGEPAGVVEQDLLVAAVRAADEQDDVGLGAPRV
                     KAPVVLAGNADAVPEAAALLRASGCRVTVTGNVLPEIGVIHPEPARSAIREVFLRHVI
                     GGKGLSTGPRFASLVRAATPDAVLRGVEVLADTLVGDILVIDIGGATTDVYSCLTPEG
                     EDASLRRNVVAPLWRARTVEGDLGMRWNARGIIEAAVREGMPPAPELVAWAERVASEP
                     SYLPIDEREATLDRELARLAAVVAVRRHARPALAAEEPRPLANVVHVIGSGGVLRYGD
                     PEGADDVLRAVTTDYAGGWRVPREAAVRVDRAYLLFAVGLLAHAGYGVQAALLAGRHL
                     GVAHP"
     gene            complement(1253..>1723)
                     /locus_tag="VHM65_04210"
     CDS             complement(1253..>1723)
                     /locus_tag="VHM65_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704939.1"
                     /translation="FGPQPVRVVLVRDKAATGYDIALVSTDPAATAAQIIERYAARWS
                     IEVAIEDAKQTTGVGQARNRLATAVERTVPFELVITSLAICWYATTGYQPGDVTAARD
                     LAPWYRDKTQPSVADMLTKLRRVIIAAQLRPADPQPATPNEINILRLAWENTAA"
BASE COUNT          204 a          572 c          672 g          275 t
ORIGIN      
        1 ttgccggcca ggacgaccgg tgccttgacg cgcagggcgg cgagccggcg ggcgttgtgg
       61 agcaggacct cctcgttgcc gccgtccgtg ccgccgacga gcaggacgat gtcgggcttg
      121 gcgccccgcg cgtcaaggca ccggtcgtcc tggccggcaa cgccgacgcg gttcccgagg
      181 ccgccgcgct gcttcgcgcg agcggctgcc gggtgaccgt gaccggcaac gtgctgcccg
      241 agatcggtgt gatccacccc gagccggcca ggtcggcgat ccgggaggtg ttcctgcgcc
      301 acgtcatcgg cggcaagggg ctcagcacgg gcccccgctt cgcctccctc gtgcgcgccg
      361 cgacacccga tgcggtcctg cgcggtgtgg aggtgctcgc ggacaccctc gtcggggaca
      421 tcctcgtcat cgacatcggc ggcgcgacga ccgacgtcta ctcgtgcctc acccccgagg
      481 gcgaggacgc gagcctgcgc aggaacgtcg tcgccccgct gtggcgtgcc cgcacggtgg
      541 agggcgacct cggcatgcgc tggaacgcgc gggggatcat cgaggcggcc gtgcgggagg
      601 ggatgccgcc cgctccagag ctggtggcct gggccgagcg ggtggcgagc gagccgtcat
      661 acctgccgat cgacgagcgc gaggcaaccc tcgaccgtga gctggcccgg ctcgcggccg
      721 tcgtcgcggt ccggcgccac gccaggcccg ccctcgctgc cgaggagccg aggccactgg
      781 ccaacgtcgt ccacgtcatc ggctccgggg gggtcctgcg ctacggcgac ccggaaggcg
      841 ccgacgatgt cctgcgcgcg gtgacgaccg actacgcagg ggggtggcgg gtgccgcggg
      901 aggccgccgt gcgggtcgac cgcgcctacc tcctcttcgc cgtcggcctg ctcgcccacg
      961 cggggtatgg cgtccaggcg gcgctgctcg ccggccgcca tctcggggtg gctcacccgt
     1021 agcggcgtcg agcctgtcgg tggcctttgg ccagggttgc tcccgtgaca gatgcagtgg
     1081 tggccgaggg gctcgtcaag cactacggca cgatgaaggc gctcgacggg gtgagtctgc
     1141 gcgtcggtga agggacgatc ctcggcgtgc tcgggtccaa cggcgcgggc cacgacgcgc
     1201 cggctgctca cgccgtttta gccagggcgc tcgtgttcct cgactttcgc agttatgcgg
     1261 cggtgttctc ccaggccagc cgaaggatgt tgatttcgtt gggagtggcc ggctgggggt
     1321 ctgctggccg taattgtgcg gcgatgatca cccggcggag cttggtgagc atgtcggcga
     1381 ccgagggttg ggtcttgtcg cggtaccagg gggcgaggtc gcgtgctgcg gtgacgtcgc
     1441 cgggctggta gccggtggtg gcgtaccagc agatcgccag gctggtgatg accagctcga
     1501 acgggacggt gcgttctacc gcggtggcca gccggttgcg ggcttggccg acgccggtgg
     1561 tctgtttcgc gtcttcgatg gcgacctcga tgctccagcg ggcggcgtag cgttcgatga
     1621 tctgcgcggc ggtcgcggcc gggtcggtgc tgaccagggc gatgtcgtag ccggttgcgg
     1681 ccttgtcgcg gaccaggacg acccgcaccg gttgggggcc gaa
//
LOCUS       DATAIX010000283         3442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_15338, whole genome shotgun sequence.
ACCESSION   DATAIX010000283 DATAIX010000000
VERSION     DATAIX010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15338"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1664)
                     /gene="thrS"
                     /locus_tag="VHM65_04215"
     CDS             complement(<1..1664)
                     /gene="thrS"
                     /locus_tag="VHM65_04215"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX2704940.1"
                     /translation="MSAPIIVSVAGSERSVAAQTTAGGLFEGERGVVVARVNGELRDL
                     AHVLDAGDVVEPVTVDSPDGLAVLRHSCAHVLAQAVQQVNPAARLGIGPPIRDGFYYD
                     FDVETPFTPEDLKALERAMQKIINEGQVFVRREISDADARAELAAEPYKCELIGLKGG
                     TAPGDLESSAEGASVEVGGAQLTIYDNVRRDGSRAWGDLCRGPHLPSTKLLGNAYKLM
                     RSAAAYWRGSERNPQLQRIYGTAWPTKPDLTAYLDRLAEAERRDHRKLGVELDLYSFP
                     DELGSGLAVFHPKGGIVRREMEGYVRQRHIDSGFDFVNTPHISKEGLFQTSGHLPYYA
                     DTMFPPMSMEGSDYYLKAMNCPMHNLIFRSRGRSYRELPLRLFEFGSVYRFEKSGVVH
                     GLTRVRGMTQDDSHTYCTPEQAPAEITMLLDFVLGLLRDFGLDDFYLELSTRDASSDK
                     FIGSDGDWAVATAILHDTAVASGLELVDDPGGASFYGPKISVQARDAIGRTWQLATIQ
                     YDFNQPERFGLEFQAADGSRRRPVMIHSAKFGSLERFMALLVEHYAG"
     gene            complement(1782..2150)
                     /locus_tag="VHM65_04220"
     CDS             complement(1782..2150)
                     /locus_tag="VHM65_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704941.1"
                     /translation="MTRRLHGAVTSIFIYPDPDGPGQEVTEAEVYETGLDGDRPKKAP
                     LSLVTLDEYVASHPRANVVVDLSSEELDGLVGAVVQIGEVRLTLTERKASCGVVYATV
                     AATGVLSAGAVLDVAGNAGD"
     gene            2208..2798
                     /locus_tag="VHM65_04225"
     CDS             2208..2798
                     /locus_tag="VHM65_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704942.1"
                     /translation="MTERPDGLEDPMTTTPPAPSALPPEDERRLAVALFNGVWALMDQ
                     EGRSARDEDRMVHMAHASRYHWEQVGPAAHLARGEWLCSRVYAVLRRAEPCLHHAQRA
                     LDICADEGIGDWDLAFAYEALARGHAVAGDGEKARTLTGLCSWPTWRRSRGSDASGEP
                     GCRTPRTRLRPSPPRGRGPARRSCASSGRCRGCRTG"
BASE COUNT          509 a         1161 c         1250 g          522 t
ORIGIN      
        1 ccggcatagt gctcgacgag gagggccatg aaacgctcga gcgacccgaa cttcgccgag
       61 tggatcatga ccggccgtcg gcgcgagccg tcggccgcct ggaactccag gccgaagcgc
      121 tcgggctggt tgaagtcgta ctggatcgtc gccagctgcc acgtgcggcc gatggcatcg
      181 cgcgcctgga ccgagatctt cgggccgtag aacgacgcgc caccggggtc gtcgacgagc
      241 tcgaggcccg acgcgaccgc cgtgtcgtgc aggatcgccg tcgcgaccgc ccagtccccg
      301 tcggagccga tgaacttgtc cgaggaggcg tcgcgcgtcg acagctcgag gtagaagtcg
      361 tccaggccga agtcgcgcag cagcccgagg acgaagtcca gcagcatggt gatctcggcc
      421 ggtgcctgct ctggcgtgca gtaggtgtgc gagtcgtcct gcgtcatccc ccgcacccgc
      481 gtcagcccgt gcacgacgcc cgacttctcg aagcggtaga ccgacccgaa ctcgaacagc
      541 cgcagcggca gctcacgata ggaccgcccg cgcgagcgga agatgaggtt gtgcatgggg
      601 cagttcatgg ccttgaggta gtagtcgctg ccctccatgg acatcggcgg gaacatcgtg
      661 tccgcgtagt acggcaggtg cccggacgtc tggaacagcc cctccttcga gatgtggggc
      721 gtgttgacga agtcgaagcc ggagtcgatg tgccgctggc gcacatagcc ctccatctcg
      781 cgccgcacga tgccaccctt gggatgaaag acggcgagcc ccgacccgag ctcgtccggg
      841 aacgagtaga ggtcgagctc gacgccgagc ttgcggtggt cgcgccgctc cgcctcggcg
      901 aggcggtcga ggtaggccgt gaggtcgggc ttggtcggcc acgccgtccc gtagatccgc
      961 tgcagctggg ggttgcgctc cgagccgcgc cagtatgccg cggcgctgcg catcagcttg
     1021 taggcgttgc cgaggagctt ggtgctgggc aggtgcgggc cgcggcacag gtcgccccag
     1081 gcccgcgacc cgtcgcggcg gacgttgtcg tagatcgtca gctgcgcgcc gccgacctcg
     1141 accgaggctc cctctgccga gctttccagg tcccccgggg ccgtcccgcc cttgaggccg
     1201 atgagctcgc acttgtaggg ctcggcggcc agctcggccc gtgcgtcggc gtcgctgatc
     1261 tcccggcgca cgaacacctg accctcgttg atgatcttct gcatcgcccg ctcgagcgcc
     1321 ttgaggtcct ctggcgtgaa cggcgtctcg acgtcgaagt cgtagtagaa cccgtcgcgg
     1381 atcggcggcc cgatgccgag cctggccgcc gggttgacct gctgcaccgc ctgcgcgagc
     1441 acgtgggcgc acgagtgacg cagcaccgcg agcccgtccg gggagtcgac cgtcacgggc
     1501 tcgaccacgt ccccggcatc gagcacgtgg gcgaggtcgc gcagctcacc gttgacccgg
     1561 gccacgacga cgccgcgctc gccctcgaag agcccgcccg ccgtggtctg cgccgcgacc
     1621 gatcgctcgc ttccggcgac ggacacgatg atgggggcag acacggtcgt tctccaatac
     1681 agacgggtcg gggcaatgca gacgggtcgg gacgagcggg cagatcctcg ggtatgacgg
     1741 gtggctggca cccccggaaa gcctagcggc cgggcgcagg ctcagtcgcc cgcgtttccc
     1801 gccacgtcga ggaccgcgcc ggccgagagg actccagtcg cggcgaccgt ggcatagacg
     1861 acgccgcacg atgccttgcg ctcggtcagc gtgagccgga cctcgccgat ctggacaacg
     1921 gctccgacga gcccgtcgag ctcctcgctc gacaggtcga cgacgacgtt cgcgcgtggg
     1981 tgggacgcga catactcatc gagggtgacg agcgacaggg gcgccttctt ggggcggtcc
     2041 ccgtcgaggc cggtctcgta cacctcggcc tcggtgacct cctgcccggg accgtcggga
     2101 tccgggtaga tgaagatcga tgtcaccgca ccgtggagcc gtcgcgtcat accgctcagg
     2161 ctacgtccct tgtccctccc cccgggaagg gctctaccgt gttgctcgtg accgagcgac
     2221 ccgacggact ggaggacccg atgacgacca ccccacccgc gccgagcgcg cttccgcccg
     2281 aggacgagcg ccgcctggcc gtcgcgctgt tcaacggcgt ctgggcgctc atggaccagg
     2341 aaggccgttc ggctcgagat gaggaccgga tggtgcacat ggctcacgcc tcgcgctacc
     2401 actgggagca ggtcggcccg gcggctcacc tggcccgcgg cgagtggctc tgctcccgcg
     2461 tgtatgccgt gctccgccgc gccgagccgt gcctgcacca cgcccaacgg gcgctggaca
     2521 tctgcgcaga cgaggggatc ggcgactggg acctggcctt cgcctacgaa gcgctcgccc
     2581 gcggccatgc ggtggccggc gacggcgaga aggcgcgcac gctgaccggg ctctgctcat
     2641 ggccgacctg gagacgatcc cggggcagcg acgcttctgg tgagccaggg tgtaggacgc
     2701 cgaggacacg tctgcggcca tcgccgccgc gaggtcgggg tccggcgcgt cggtcatgtg
     2761 cgtcgagcgg ccggtgtcgc ggttgtcgaa ccgggtgacc cacagccggt gcgcgaccag
     2821 cgcgtcacac aagccgactg gccaggcgat catctgcgcg ccgcccccat cgtgagcagc
     2881 acgggtgggt cgctcgggtc gccgaaggac tcgtaggcaa gggtggtgtc cgacggcccc
     2941 acgtgcaacg gagtgttcgc cgcacctggc acggtgccga gtcggggtct ccggcagcca
     3001 agggcacaca cgggttcggt gtcgaagccg tgcgccctac cgttcacggg acgcgatcga
     3061 gacggggcgt cagggcagac ccggccggga tccagctcca cgctgggata cacgagttgg
     3121 gcgccgtcca tcggcccggt gaacgttcgg tgggctgctc agtcatgctt cgagtctggt
     3181 gtagcgccga ggcttcgtac ctgcgaggcg cggcatgagg gtgcggtgga ggcactggtc
     3241 cagcccggtc gccgcccgat cgccggggcc gggagtacgg caacccgtcc tggctatgct
     3301 gggtcgtggc gagcgactgc cggcgcggtt ggttgctgga ttcgtgaggc ggcggctgag
     3361 aggcccaggc gggaggacgg ttcgatgcga gcgctgcagt tcaggatgta tggcggtccg
     3421 gaggtcctgg agtggggtga gg
//
LOCUS       DATAIX010000284         1619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_105098, whole genome shotgun sequence.
ACCESSION   DATAIX010000284 DATAIX010000000
VERSION     DATAIX010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105098"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VHM65_04230"
     CDS             <1..1068
                     /locus_tag="VHM65_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704943.1"
                     /translation="FLRWNSIVNLGIGMPEGVAAVAGEEEVLDLITLTVEPGAIGGLP
                     AGGLSFGAATNPQSIIDQASMFDFYDGGGLDQAFLGFAEVDAQGNVNVSRFGSRLAGA
                     GGFINISQNAGALYFLGTFAAHSQEEVVDAELRIHHPGRTPKFVPRVSHVTFSAEQAR
                     SRDQEVRYITERCVLRLAADGLEVIEVAPGVDLERDVLAQLPFRPTVSPRLRTMDAAI
                     FCPGRMGLRHDEVLSLRERVLYRPDVDLLYLNFEGLQLDTEQDADDLARELEAELKSY
                     GRKLAAVVNYDNFRLAPPAADRYWAMIRRNTTHYLSSTTRYSTNAFFRHQLGESFSAA
                     SLDGHVYRSFDEAVHHLDNGG"
     gene            1093..>1619
                     /locus_tag="VHM65_04235"
     CDS             1093..>1619
                     /locus_tag="VHM65_04235"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX2704944.1"
                     /translation="MDDTPDLDGSDLDGSDLADLAALRDLLASAPLIDGHNDLLWELR
                     KQVGYDLDRLDISRPAASVQTDLPRLRAGGVGAQFWSVYVRSDMPGDTAVTATLEQLD
                     GWRALVARYPDDLQLAVCADDVDRALATGRIASLAGMEGGHSIGCSMGALRAMYALGA
                     RYLTLTHNDNTPWADS"
BASE COUNT          241 a          585 c          553 g          240 t
ORIGIN      
        1 ttcctgcgct ggaactccat cgtgaacctc ggcatcggga tgcccgaggg ggtcgccgcg
       61 gtagccggcg aggaggaggt gctcgacctg atcacgctca cggtcgagcc cggcgcgatc
      121 ggtggcctgc ccgcgggcgg gctctcgttc ggggccgcca ccaacccgca gtcgatcatc
      181 gatcaggcct cgatgttcga cttctacgac ggcggcggtc tcgaccaggc cttcctcggg
      241 ttcgccgagg tcgacgcgca gggcaacgtc aacgtgagcc ggttcggcag caggctggcc
      301 ggggccggcg ggttcatcaa catcagccag aacgccggcg cactgtactt tctcggcacc
      361 ttcgcggccc actcgcagga ggaggtcgtc gacgccgagc tgcggatcca tcaccccggc
      421 cggacgccga agttcgtgcc gcgggtgagc cacgtgacct tcagcgccga gcaggcccgc
      481 agccgcgacc aggaggtgcg gtacatcacc gagcgttgcg tgctgcggct ggccgccgac
      541 ggcctcgagg tgatcgaggt cgcgcccggg gtcgacctcg agcgcgacgt gctcgcgcag
      601 ctacccttcc gccccacggt gtcgccgagg ctgcgcacga tggacgccgc gatcttctgc
      661 cccggccgga tggggctgcg ccacgacgag gtgctcagcc tgcgcgagcg ggtgctctac
      721 cggccggacg tcgacctgct ctacctgaac ttcgagggcc tgcagctcga caccgagcag
      781 gacgccgacg acctggcccg cgagctggag gccgagctga agtcctacgg gcgcaagctg
      841 gccgccgtcg tcaactacga caacttcagg ctcgcgcccc cggccgccga ccggtactgg
      901 gcgatgatca ggcgcaacac cacgcactac ctgagctcga ccacccggta ctcgaccaac
      961 gcgttcttcc ggcaccagct cggtgagagc ttctcggcgg cgagcctgga cgggcacgtc
     1021 taccgcagct tcgacgaggc ggtgcaccac ctcgacaacg gtggctgacc tggcataggg
     1081 caggatcgcg gcgtggacga cactcccgat ctcgacggct ccgatctcga cggttccgat
     1141 ctcgccgacc tggctgccct gcgtgacctg ctcgcctcgg cgccgctgat cgacgggcac
     1201 aacgacctgc tctgggagct gcgcaagcag gtcggctacg acctcgaccg gctcgacatc
     1261 tcccggcctg cggcatcggt gcagaccgac ctgccccggc tgcgcgcagg tggcgtcggg
     1321 gcgcagttct ggtcggtgta cgtccggtcc gacatgcccg gggacaccgc ggtcaccgcc
     1381 acgctggagc agctggacgg ctggcgagcg ctggtcgccc gctaccccga cgacctgcag
     1441 ctcgcagtgt gcgcggacga cgtcgaccgc gcgctcgcca caggccggat cgcctcgctc
     1501 gccgggatgg agggcggcca ctcgatcggc tgctcgatgg gggcgcttcg agcgatgtac
     1561 gcgctcggtg cgcggtacct gacgctcacc cacaacgaca acactccctg ggcggactc
//
LOCUS       DATAIX010000285         2750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_165018, whole genome shotgun sequence.
ACCESSION   DATAIX010000285 DATAIX010000000
VERSION     DATAIX010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165018"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VHM65_04240"
     CDS             complement(<1..387)
                     /locus_tag="VHM65_04240"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX2704945.1"
                     /translation="MSSPPGPAAESSYDEWMGMALALASGAGAAGDVPVGAVVVSAAG
                     EVIGQGANCREALADPTGHAEVVALREGARHTGSWRLTGATLVVTLEPCVMCAGALVL
                     ARVARVVIGAWDPKAGACGSVWDLVRD"
     gene            404..1042
                     /gene="upp"
                     /locus_tag="VHM65_04245"
     CDS             404..1042
                     /gene="upp"
                     /locus_tag="VHM65_04245"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF001097.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX2704946.1"
                     /translation="MRVHVADHQLITHKLTYLRDKRTDSPTFRRLAEELVTLLAYEAT
                     RGVRVEPFAIETPVGPTMGIKLAKPKPLVVPILRAGLGMLEGMVRILPSAEVGFLGMA
                     RNEETLEAVTYANRLPDDLAGRQCYVLDPMLATGGTLAMAITYLVERGADDITAICLL
                     AAPEGIEAVTREVDGIGVPVTLVTGAVDERLNEHGYIVPGLGDAGDRLYGVV"
     gene            1150..1578
                     /locus_tag="VHM65_04250"
     CDS             1150..1578
                     /locus_tag="VHM65_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704947.1"
                     /translation="MTSANIGSALRVMEVGDTFTVTFSEPLNPRVVLAANAKQTDPSG
                     PGNDLLTIVGLTNGALDLGADTFVTPDGGSIVYPDSTMTMLNANTAVRSAIVGECSGS
                     ACGSNGPGATASLSFEPEPFIKDLNGNPAEGTLTQVVTLY"
     gene            1699..1866
                     /locus_tag="VHM65_04255"
     CDS             1699..1866
                     /locus_tag="VHM65_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704948.1"
                     /translation="MPRIRKILLWVLFAFIVYAVYSSPAQAGDIVRSAWEILGNAVTA
                     IGRFFRSMLGQ"
     gene            1873..2550
                     /locus_tag="VHM65_04260"
     CDS             1873..2550
                     /locus_tag="VHM65_04260"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX2704949.1"
                     /translation="MRIARPHRSGSPDLDRYLLEGERLVLAVHQHWGKVAESVASAVA
                     AVVVAVWLDVSLPGRLGAVGTVVWLAATALAFRAFWTVYERGRDWFVVTDKRLLMIKG
                     WYTRDVPMMPLVKVTDMSYKRSLPGRIFGYGRFVMESAGQDQAMREIEWVPNPDHTYR
                     VIISVIFGVGGGETPAADEVDPPYAGWRDPARAESIYRSEDVQARERAADTAHQGLSR
                     LRLSPKH"
BASE COUNT          427 a         1036 c          896 g          391 t
ORIGIN      
        1 gtcccgcacc aggtcccaga ccgagccgca tgcccccgct ttggggtccc aggcgccgat
       61 gaccacccgc gccacccgag ccaggaccaa agccccggcg cacatgacgc acggctcgag
      121 ggtcaccacg agggtggcgc cggtcaaccg ccacgagccg gtatggcggg caccctcgcg
      181 cagtgccacg acctcggcgt gccccgtcgg gtccgcgagc gcctcacggc agttcgcgcc
      241 ctggccgatc acctcgccgg cagccgagac caccaccgcc ccgactggga cgtcgcctgc
      301 cgcgcccgcg cccgacgcga gggcgagcgc catacccatc cactcgtcat acgaggactc
      361 ggcggcgggg ccgggaggcg agctcacccg ctaagtttct cccatgcgcg tccacgtggc
      421 cgaccaccag ctcatcaccc acaagctcac ctacctgcgg gacaagcgca ccgactcgcc
      481 caccttccgt cgcctcgccg aggagctcgt cacgctgctc gcctacgagg ccacgcgcgg
      541 ggtgcgcgtc gagccgttcg ccatcgagac gcccgttggg ccgaccatgg gcatcaagct
      601 cgccaaaccc aagccgctcg tcgtgccgat cctgcgggcc gggctcggga tgctagaggg
      661 catggtgcgg atcctgccct cggccgaggt gggcttcctc gggatggcgc gcaacgagga
      721 gacgctcgag gcggtgacct acgccaaccg gctgccagac gacctcgccg gccggcagtg
      781 ctacgtcctc gacccgatgc tggccaccgg cggcaccctc gccatggcga tcacgtacct
      841 cgtcgagcgc ggcgccgacg acatcaccgc gatctgcctg ctcgccgcac ccgagggcat
      901 cgaggcggtc actcgggagg tcgacgggat cggcgtccca gtcaccctcg tcaccggggc
      961 cgtcgacgag cggctcaacg agcacgggta catcgtgccc ggcctcgggg atgcgggaga
     1021 ccgcctctac ggcgtcgtct gagaggttgc cttgcatcgt cgccgaccgg catcatcgat
     1081 gccgagggcg accagggctc gttcgccgca acggcccccg ccgaccgcat gggtcccgtg
     1141 cccgtgagca tgacgagcgc gaacatcggc tcggcgctga gggtcatgga ggtcggcgac
     1201 acgttcaccg tcaccttcag cgagcccctc aacccgcgag ttgtcctcgc ggccaacgcc
     1261 aagcaaacgg acccgagcgg gccagggaac gacctgctca ccattgtggg tctgaccaac
     1321 ggtgctctcg acctcggagc agacacgttc gtcactcccg atggtggcag catcgtctac
     1381 cccgactcga ccatgaccat gctcaacgcc aacacggcgg tccggtcggc catcgtcggg
     1441 gagtgcagtg gcagcgcctg cgggagcaat ggcccggggg cgacggcaag tctgtcgttc
     1501 gagcccgaac cgttcatcaa ggatctcaat ggcaacccag ccgaagggac gctgacccag
     1561 gtcgtgacgc tctactgaca cagcgctgtg agctggccag tgaggcgagc cgggcccgtc
     1621 ccggcgcgcc tcactggcca cactcgtcac ggctgcacac tcgggccacg tggtgtgttc
     1681 gacggccaag gagttcccat gccccgcatc aggaagatcc tgctctgggt cctctttgcc
     1741 ttcatcgtgt atgccgtgta cagctccccg gcccaggctg gcgacatcgt gcgcagcgcc
     1801 tgggagatcc tcgggaacgc cgtcaccgcc atcggccggt tcttccgctc gatgctgggc
     1861 cagtagccac ccgtgcgcat cgcccggccc caccgcagcg gctcgcccga cctcgaccgc
     1921 tacctcctcg agggcgagcg actggtcctc gccgtccacc agcactgggg caaggtcgcc
     1981 gagtcggtgg ccagcgccgt ggctgcggtg gtcgtggccg tctggctcga cgtgagcctg
     2041 ccgggccgcc tcggcgcagt cgggaccgtc gtctggctcg ccgccaccgc cctggccttc
     2101 cgtgccttct ggacggtcta cgagcggggt cgcgactggt tcgtcgtcac cgacaagcgg
     2161 ctgctcatga tcaagggctg gtatacccgc gacgtcccga tgatgccgct ggtcaaggtg
     2221 acggacatgt cctacaagcg ctccctcccg ggccggatct tcggctacgg gcgcttcgtc
     2281 atggagagcg ccggccagga ccaggccatg cgcgagatcg agtgggtccc gaaccctgac
     2341 cacacgtacc gcgtcatcat ctcggtcatc ttcggggtcg gcggcggcga gacccccgct
     2401 gcagacgagg tcgacccccc gtatgccggg tggagggatc ccgcacgggc ggagtcgatc
     2461 taccgcagtg aggacgtgca ggcgcgcgag cgcgcggcgg acaccgccca tcagggtcta
     2521 tcccgactcc ggctgagccc gaagcactga gcccgaggcg ctcagcccga gccgcccagc
     2581 ccagcccgaa gcactgagcc cgaggcgctc agcccgaggc gctcagccca gcccgaagcg
     2641 ctcagcccga ggcgctcagc ccgaggcgcc gcgcggcggc ccctgcaaac cccttgtccg
     2701 gtatggcgct tcgctcgcct cgagtgttgg acgaccgcca ggccgcccac
//
LOCUS       DATAIX010000286         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_165042, whole genome shotgun sequence.
ACCESSION   DATAIX010000286 DATAIX010000000
VERSION     DATAIX010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165042"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1258
                     /gene="hppD"
                     /locus_tag="VHM65_04265"
     CDS             53..1258
                     /gene="hppD"
                     /locus_tag="VHM65_04265"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX2704950.1"
                     /translation="MKTASHLTQQERDARLDLEALKTLVGLVEYDESTDVFPVTGWDA
                     IVMVVGNATQTAHFYQSAFGMKLVAYCGPETGTRDHKAFVLRSGSVRFVIKGGVHPGS
                     PLLDHHRRHGDGVVDIALEVPDVDRCVAQARREGARILEEPHDLTDEHGTVRMAAIGT
                     YGETRHTLVDRSRYAGAYLPGYVARTSAFVPRDGAPTRLFQAVDHIVGNVELGQMDTW
                     VGFYNRVMGFVNMAEFIGDDIATDYSALMSKVVANGNHRVKFPLNEPAVARRKSQIDE
                     YLEFYRGPGAQHLALATNDILATVDAMRAEGIEFLDTPDAYYEDPDLRARIGHVRVPV
                     EELQSRGILVDRDEDGYLLQIFTTPIGDRPTVFFELIERHGSLGFGKGNFRALFESIE
                     REQERRGNL"
     gene            1283..>1572
                     /locus_tag="VHM65_04270"
     CDS             1283..>1572
                     /locus_tag="VHM65_04270"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HEX2704951.1"
                     /translation="MSVPSGWYDDPENPENLRYWDGVLWTGHRQPKKSPTADQSRIGR
                     PYAVAPADHPGVSGTRPVDQPGPGSPPQSPWTAPSAGQGTQPTYGQPSYPPVA"
BASE COUNT          263 a          561 c          530 g          218 t
ORIGIN      
        1 ctcccgcaca gtgttcggat gaggacagtg ttcggatgag gacagtgtct ggatgaagac
       61 agcaagccac ctgacccagc aggagcgcga cgcccgcctc gacctcgagg cactcaagac
      121 cctcgtcggg ctcgtcgagt acgacgagtc cacggacgtc ttcccggtca ccgggtggga
      181 cgcgatcgtc atggtcgtcg gcaacgccac ccagacagcg cacttctacc agagcgcgtt
      241 cgggatgaag ctggtcgcct actgcggtcc cgagacgggc acccgggacc acaaggcgtt
      301 cgtgctcagg tcggggtcgg ttcggttcgt catcaagggc ggcgtccatc ccggcagccc
      361 gttgctcgac caccaccgtc gccatggcga cggcgtcgtc gacatcgccc tcgaggtccc
      421 tgacgtcgac cggtgcgtcg cccaggcgcg ccgggagggc gcgcggatcc tcgaggagcc
      481 ccacgacctg accgacgagc acggcaccgt ccgcatggcc gccatcggca cctacggcga
      541 gacgcgccat accctcgtgg accgcagccg gtatgccggg gcctaccttc ccgggtacgt
      601 cgcccgcacg tcggcgttcg tcccgcgcga cggggcgccc acccgtctct tccaggcggt
      661 cgaccacatc gtcggcaatg tcgagctggg ccagatggac acctgggtcg ggttctacaa
      721 ccgggtcatg gggttcgtca acatggccga gttcatcgga gacgacatcg cgaccgacta
      781 ctcggcgctc atgagcaagg tcgtcgccaa cggcaaccac cgggtcaagt tcccgctcaa
      841 cgagccggcc gtcgcccggc gcaagagcca gatcgacgag tacctcgagt tctaccgggg
      901 cccgggcgcc cagcacctcg cacttgcgac gaacgacatc ctcgcgaccg tcgacgcgat
      961 gcgggccgag gggatcgagt tcctcgacac accggacgcc tactacgagg accccgacct
     1021 gcgggcgcgc atcggccacg tccgggtgcc ggtcgaggag ctgcagagcc ggggcatcct
     1081 cgtcgaccgg gacgaggacg ggtacctgtt gcagatcttc accacgccga tcggtgaccg
     1141 gcccacggtc ttcttcgagc tcatcgagcg gcacgggtcg ctcggcttcg gcaagggcaa
     1201 tttcagggcg ctcttcgagt cgatcgagcg cgagcaggag cgccgcggca acctgtaacc
     1261 gcggccgtgg gacgatgggc gcatgagcgt tccgtcaggg tggtacgacg acccggagaa
     1321 ccccgagaac ctgcggtact gggacggcgt cctgtggacg gggcaccgcc agcccaagaa
     1381 gtcgccgacc gccgaccagt cgcggatcgg gcggccctat gcggtggcgc cggcggacca
     1441 ccccggcgtg agcggcacca gacccgtgga ccagcccggg ccggggtcgc cgccccagtc
     1501 gccgtggacc gccccgtcgg ccggccaggg cacccagccg acctacgggc agccgtcata
     1561 cccgccggta gc
//
LOCUS       DATAIX010000287         4184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_275074, whole genome shotgun sequence.
ACCESSION   DATAIX010000287 DATAIX010000000
VERSION     DATAIX010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275074"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..421)
                     /locus_tag="VHM65_04275"
     CDS             complement(143..421)
                     /locus_tag="VHM65_04275"
                     /inference="COORDINATES: protein motif:HMM:NF025385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HEX2704952.1"
                     /translation="MGAVVWKVLGTGSAILAAAVATRLVQAAWASVGRDIPKDPENPY
                     ETGWGEALTFAAASGLAMGVARVAASRKAAEYYTRSAGHPPKALTSKD"
     regulatory      521..627
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            716..1366
                     /locus_tag="VHM65_04280"
     CDS             716..1366
                     /locus_tag="VHM65_04280"
                     /inference="COORDINATES: protein motif:HMM:NF021348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEX2704953.1"
                     /translation="MATHHTTGRAARARTPILASRPLAAYRTIDLITLAFLGVAFGLA
                     FWAWGKAYAAPAAGLAAVFPPLQGVTSAPWLMAGVVGGLVVRRPGAALFTELIAAVVS
                     MLPGTQWGAEVFVSGLVQGLGAEIGFALLAYARYGPVAAALAGACAAPLEAVYEWYAY
                     WTDWGWSYKVAYLLILTVAGAAVAGLGGWALTRALARSGALAALPPGLEARAERAV"
     gene            1363..3711
                     /locus_tag="VHM65_04285"
     CDS             1363..3711
                     /locus_tag="VHM65_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2704954.1"
                     /translation="MTLTSPRARGPAGHLAPGAPPGPPGVLAPPGAKGRVELDALTWR
                     PVGRRQPVLDAVSLSIAPGERVLLAGASGSGKSTLLRALAGLLLTADSGDLSGTVRID
                     GAAPGAIPGQVGLVLQEPGSGVVAGTVERDVAFGLENLGEPSESMPGQVAQSLTEVRL
                     GMPSRSSTGALSGGEHQRLALAGALAMGPDLLLLDEPTAMLDAASADAVRDAVDRLTR
                     ARGLTLVVVEHRLGPWLPLVDRLVVLHADGHILADGVPQQVLAGHGADLLEAGLWLPG
                     APAPDPLDLGDLLPGPAGGVDRPVMSARDVTIIHRSTTVGGSTRVTTAVRDADVDLTA
                     SDLVAIVGPSGSGKSSLLAALAGLVTPVSGVVEAAAQLAPGPGRRDPARWSSPELARR
                     VAWVPQNAASTLIASTVLDEVMATPRALGLDEAPFLGRAHGLLDRLGLAALAGADPRH
                     LSGGEQRRLAVAAAAVHEPALVLADEPTVGQDRHTWAAVVGVLRALQSGGSALAVTTH
                     DEDLVRAADTVLALSTPAGAIRQPGPSARRPLVTRAGPLSLLLATLVVLPLPALIDSW
                     RQALSVLAVEAALTAVALAAPGRGAPPSGRLRLLVTRMWPPALGMVTVAWSVWLLGGH
                     DIEAAATAALRVLALVYPGSVVLPYLDPDQLGDHLAQRLHLPARPVVAATAALGRFQS
                     FGALWGQVSSARRVRGVGGARGLLGRGREVVAVTVALLGGALGSAAVLALAMDARGFA
                     AAHRRTWFVGAPWRRADTAVIAAALAVVAAAACARTVLPLVP"
     gene            3773..>4184
                     /locus_tag="VHM65_04290"
     CDS             3773..>4184
                     /locus_tag="VHM65_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704955.1"
                     /translation="MFGTVSPSARRTAAAGAVLAAALAAAACSGSGTTSSPSGSASGS
                     TPPASSSPSASPTGSITGTVTPGPKNDLAKRKNVQLKGCTVSKTGGEASGVITNKGAK
                     AVVYTITVVFTNDKASNVGAETRKVIAAAGKDTTW"
BASE COUNT          448 a         1607 c         1525 g          604 t
ORIGIN      
        1 cagcctgctc ctgctcgcga tggtcttcgt cctgctggcc cggcgccgca gcacccgccg
       61 acgcgccgac tgagtgcctc tgcccccagc ggagccggca agcgcgaccg atgcccggcg
      121 ggagcgggtc agtccttgct ggtcagtcct tgctggtcag cgccttgggc gggtgccccg
      181 ccgacctggt gtagtactcg gccgccttgc gcgacgccgc cacgcgggcc acgcccatgg
      241 cgagcccgct ggccgcggca aaggtgaggg cttcgcccca gcccgtctcg tacgggttct
      301 cggggtcctt cgggatgtcg cggccgacac tggcccaggc ggcttggacc agcctggtcg
      361 ccaccgccgc cgccaggatc gccgacccgg ttccgaggac cttccacacg actgcgccca
      421 tcgcgagctc cctccgacgc tgtgcctacg acgctgtgcc tccgacgtgg tgcctacgac
      481 gctgactgtg tcacgcccgg gggtgtaccg tcatcttcac gaacaacgac aggggagcgc
      541 cccaagcgct gagagtgcgg cccccggccg cagaccctcg aacctgatcc ggctagtacc
      601 ggcgaaggga gtcgggacat ctcaggtgcg ttcccgtcat accggctcgc tccggtatgg
      661 cgggcacaca cctccccgag gtatccatcc ggagaccacg ggaaacgagg accacatggc
      721 aactcaccac accaccggcc gtgctgcccg cgcccgcacg ccgatcctgg ccagccggcc
      781 gctggcggcc taccgcacga tcgacctcat cacgctcgcc ttcctcggcg tcgcgttcgg
      841 cctcgcgttc tgggcctggg gcaaggccta tgccgcgccc gcggcgggcc tcgctgcggt
      901 gttcccgccc ttgcagggcg tgacgagcgc gccgtggctc atggccggag tcgtcggcgg
      961 cctcgtcgtg cgccgcccgg gcgcggcgct gttcaccgag ctcatcgcgg ccgtcgtctc
     1021 gatgctcccg gggacccagt ggggcgccga ggtgttcgtc tccgggctcg tgcaggggct
     1081 cggcgccgag atcgggttcg cgctgctggc ctacgcgcgg tacggcccgg tcgccgccgc
     1141 cctcgcggga gcctgcgcgg ccccgctcga ggcggtctac gagtggtacg cctactggac
     1201 cgactggggc tggagctaca aggtggccta cctgctcatc ctcaccgtgg ccggtgctgc
     1261 cgtcgcgggt ctgggcggct gggcgctgac ccgcgccctg gctcggtcgg gggcgctggc
     1321 ggcgctcccc ccgggcctgg aggctcgagc cgaacgggcg gtatgacgct cacctcaccc
     1381 cgggcgcgcg gtccggccgg tcacctcgcg cccggcgccc cgccggggcc gcccggcgtg
     1441 ctcgccccgc ccggggctaa gggccgggtc gagctcgacg cgctcacctg gcgtccggtc
     1501 gggcgccggc agcccgttct cgacgccgtg tcgctgtcga tcgcccctgg tgagcgggtc
     1561 ctgctggccg gagcgagtgg ctcgggcaag tcgacgctgc tgcgggcact ggctgggctg
     1621 ctgctcaccg ccgactcggg cgacctgagc gggacggtcc ggatcgacgg cgccgccccg
     1681 ggcgcgatac cgggtcaggt ggggctggtc ctgcaggagc cgggttccgg cgtcgtcgca
     1741 gggacggtcg agcgcgatgt cgccttcggc ctggagaacc tcggcgagcc gtccgagtcc
     1801 atgccaggcc aggtcgcgca gtctctgacc gaggtcaggc tcggtatgcc gtcgcgctcg
     1861 tcgaccggcg cgctgtccgg cggggaacac cagcgcctgg ccctggccgg tgcgctggcc
     1921 atgggcccgg acctcctgct gctcgacgag ccgaccgcca tgctcgacgc ggcctcggcc
     1981 gatgccgttc gcgacgcggt tgaccgactc acccgggcgc gggggctcac gctcgtggtc
     2041 gtcgagcacc ggctcggacc ctggctgccc ctcgtcgacc ggctggtcgt cctgcacgcg
     2101 gacgggcaca tcctggccga cggtgtgccg cagcaggtgc tcgccggcca cggcgccgac
     2161 ctcctcgagg ccggcctctg gctgccgggg gctccggccc ctgacccgct cgacctcggc
     2221 gacctgctcc ccggcccggc ggggggggtc gatcggcccg tgatgagcgc ccgagacgtc
     2281 acgatcatcc accggagcac gacggtcggc gggtccaccc gagtgacgac ggcggtgcga
     2341 gacgctgacg tcgacctcac cgcgtcggac ctggtggcga tcgtcgggcc gagcgggtcg
     2401 ggcaagtcct cgctgctggc cgcgctcgcc gggctcgtga cgccggtcag tggcgtcgtc
     2461 gaggcggccg cgcagctcgc gcccgggccc ggccggcgtg acccggcccg gtggtcctca
     2521 cccgagctcg cccggcgcgt ggcctgggtg ccccagaacg ccgcctcgac actcatcgcc
     2581 tcgaccgtcc tcgacgaggt gatggcgacc ccccgggcgc tcgggctcga cgaggccccc
     2641 ttcctcggcc gggcccacgg gctgctcgac cggctcgggc tggcagcgct cgccggggcc
     2701 gacccacggc acctgtccgg aggcgagcag cgccggctcg cggtcgccgc cgcggccgtg
     2761 cacgagccgg ccctcgtcct cgcggacgag cccacggtcg gccaggaccg ccatacctgg
     2821 gccgccgtcg tcggcgtcct gcgggcactg cagtccggcg gctccgctct ggccgtgacg
     2881 acccacgacg aggacctcgt gcgcgcggcc gacacggtgc tggcgctgtc cacccccgca
     2941 ggcgcgatac ggcagccggg accatcggcc cgccggccct tggtcacccg ggccgggccg
     3001 ctctcactgc tgctcgccac gcttgtcgtg ctcccccttc cggccctgat cgactcctgg
     3061 cgtcaggcgc tgtcggtgct cgccgtcgaa gcagccctca ccgcagttgc cctcgccgcc
     3121 cccgggcgcg gcgccccgcc atccgggcgt ctgcggctgc tcgtgacccg gatgtggccg
     3181 ccggcgctcg gcatggtcac ggtggcgtgg tcggtgtggc tcctcggcgg tcacgacatc
     3241 gaggcggcgg cgacggccgc gctgcgcgtg ctcgccctcg tctatcccgg gtccgtcgtg
     3301 ctgccctacc tcgacccgga ccagctgggg gaccatctgg cccagcggct ccacctgccg
     3361 gcccggccgg tggtcgcggc cacggcggcg ctggggcggt tccagtcgtt cggggcgctg
     3421 tgggggcagg tgtccagtgc ccggcgggtc cgaggcgtcg gcggcgcgcg tgggctgctc
     3481 ggccgggggc gggaggtggt cgccgtgacc gtggcgctgc tcggcggtgc cctcgggtcg
     3541 gcggccgtgc tcgccctcgc gatggatgcc cggggtttcg ccgcagcgca tcggcggacc
     3601 tggttcgtgg gggcaccctg gcggcgagcg gacacggccg ttatcgctgc ggccctggcg
     3661 gtcgtggcgg ccgccgcctg tgcccggacc gtccttcccc tggtgccctg agcgacccac
     3721 tcgtgcttcg ccccgtagac tggcgggcag accgaccggg agggtctcgt tgatgttcgg
     3781 caccgtatct ccgtccgcgc gccgcaccgc tgccgcggga gccgtccttg ccgcggcgct
     3841 ggccgccgcg gcctgctccg gctctgggac cacgtcatcg ccgtcgggct cggcctccgg
     3901 gtcgacaccg cccgcgtcga gctcgccgag cgcgagcccg accggcagca tcaccgggac
     3961 cgtgactccg ggccccaaga acgacctcgc caagcgcaag aacgtccagc tcaagggctg
     4021 caccgtgagc aagaccggtg gcgaggcgtc cggggtcatc acgaacaagg gcgccaaggc
     4081 cgtcgtctac acgatcacgg tggtcttcac caacgacaag gcgtccaacg tgggcgcgga
     4141 gacccgcaag gtcatcgccg ccgcgggcaa ggacacgacc tggg
//
LOCUS       DATAIX010000288         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_15584, whole genome shotgun sequence.
ACCESSION   DATAIX010000288 DATAIX010000000
VERSION     DATAIX010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15584"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1509
                     /locus_tag="VHM65_04295"
     CDS             49..1509
                     /locus_tag="VHM65_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX2704956.1"
                     /translation="MTRWAPRSTRRARAISARAPAARARLVDRGAQRVMALVGGGDNG
                     GDALYAVAHLAAQGFPVAVVTVASQAHEGGLAAVAQLGTEVVALSSDPLRAHELAAEA
                     DLVVDGILGIGGRPGLGGDVETLLGAISPDAYVLAVDLPSGHDPDGAETGPGGVFADE
                     TVSFGALKGVHLLPPGERAVGRLTLVDIGLDLATSPASPSVQRLTRADVADRWPVPGP
                     DDDKYSRGVVGIVAGGPSYTGAAVLAVTSAVCSGAGMVRYVGPRLPTDLVRAAVPEAL
                     AGPGSVQAWVVGPGLDAADDSEQGRAQVSAAREALASDLPCVVDAGGLDLIAGPRSAP
                     TLLTPHAGECARLLTRLTREAVRRADVVDRPVAQARVLASLTGATVLLKGSTTVVVEA
                     DPGRSVWSQADAPPWLATAGAGDVLAGLLGTLLAAGLDPPDAGALGALVHGVAAERAN
                     PGGPVRALDVARAIPATVTSLLQYATGPFGGADLAD"
     gene            1517..>2454
                     /gene="alr"
                     /locus_tag="VHM65_04300"
     CDS             1517..>2454
                     /gene="alr"
                     /locus_tag="VHM65_04300"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX2704957.1"
                     /translation="MSALPSCARVDLDAIAANVRVLREHAPTASVMAVVKADGYGHGL
                     LPSARAALAGGASWLGVAQLDEALALRAAGITAPTLTWLHAPGAAFTDALEAGIDLGV
                     SADWAIAQVVAGARQTGRTARIHLKVDTGLSRGGVMPTDLPAALEGIGRAVAEESVEL
                     VGVWSHFSWADDPGHPTVRRQHERFVDTVRTIESAGLRPQIRHLANSAATLTNPAVHF
                     DLVRPGLAVYGLSPVPQLGGPEHFGLVPAMTLVAQLSLVKRVPRGSGVSYGHQYVTER
                     DTVLGLVPMGYADGIPRNATNVGPLAVAGARHTIAG"
BASE COUNT          287 a          912 c          904 g          351 t
ORIGIN      
        1 acggcataca gggcatctcc gccgttgtcc ccacccccga ccaaagccat gacccgctgg
       61 gctccgcggt cgacgagacg tgcccgggcg atctcggcga gggccccggc agcccgggca
      121 cgtctcgtcg accgcggagc ccagcgggtc atggctttgg tcgggggtgg ggacaacggc
      181 ggagatgccc tgtatgccgt cgcgcacctt gctgcgcaag ggtttccggt cgccgtcgtc
      241 accgtggcgt cccaggcgca cgagggcggt ctcgctgccg tggcccagct cgggaccgag
      301 gtcgtggccc tgtcgagtga cccgctccgg gctcacgagc tggctgccga ggccgacctc
      361 gtcgtcgacg ggatcctcgg catcggcgga cgtcccgggc tcggcggtga cgtcgagacg
      421 cttctcggcg cgatcagtcc cgatgcctac gtcctcgccg tcgacctgcc gtcggggcat
      481 gaccctgacg gcgccgaaac cgggccgggc ggggtcttcg ccgacgagac cgtgtcgttc
      541 ggcgcgctca aaggagttca cctgctccct cccggggagc gtgcggtcgg acggctgact
      601 ctcgtcgaca tcgggctgga cctggcgacc tcacccgcca gccccagcgt gcagcggctg
      661 actcgtgccg acgtggcgga ccggtggccg gtgccaggcc ccgacgacga caagtactcg
      721 cgcggggtcg tcgggatcgt tgcgggcgga ccgtcataca ctggggcggc ggtcctcgcg
      781 gtgacctcgg cggtgtgctc gggcgcagga atggtgcggt atgtcggccc gcggctgccg
      841 acggacctgg tccgagccgc ggttcccgag gcgcttgccg ggcccgggag cgtccaggcg
      901 tgggtcgtcg ggcccggcct cgacgccgcg gacgactccg agcagggccg ggcccaggtg
      961 tctgccgcgc gagaggccct ggcgagcgac ctgccctgtg tcgtcgacgc cggcgggctc
     1021 gacctgatcg ccgggccccg ctcggccccg accttgctca cgccccacgc cggtgagtgc
     1081 gcgcggctgc tcacccggct cacccgggag gcggtgcgga gggccgatgt cgtggaccga
     1141 ccggtggcgc aggcccgggt gctcgcctcg ctcaccggcg cgacggtcct gctcaagggc
     1201 tcgaccacgg tcgtcgtcga ggccgacccg gggcgttcgg tctggtccca ggcggacgcg
     1261 ccaccgtggc tggcgaccgc gggggccgga gacgtcctcg ccgggctgct cggcaccctg
     1321 ctcgccgcgg gcctggaccc gccggacgcc ggtgcgctgg gggctctcgt ccacggggtc
     1381 gctgcggagc gggccaaccc gggaggcccg gtccgggcgc tcgacgtggc gcgcgccata
     1441 ccggcgaccg tgacctcgct cctgcagtac gcgaccggac ccttcggggg cgcggatctg
     1501 gcagactaaa tctgatatgt ccgccctccc ttcgtgcgcc agagtcgacc tcgacgccat
     1561 cgcggcgaac gtgcgtgtcc tgcgcgagca cgcaccgacc gcgagcgtca tggccgtcgt
     1621 caaggccgac gggtacggcc atggcctgct ccccagcgcc cgggcggccc tcgcgggcgg
     1681 agcgtcctgg ctgggcgtcg cccagctgga cgaagccctg gccctgcggg ccgccggcat
     1741 cacggcgccg acgctgacct ggctgcacgc cccgggtgcc gccttcaccg acgccctcga
     1801 ggccgggatc gacctcggcg tctcggcgga ctgggcgatc gcgcaggtcg tcgcaggagc
     1861 ccggcagacc ggtcgcacgg cccggatcca tctcaaggtc gacacggggc tgtcccgagg
     1921 cggagtgatg cccaccgacc tgcccgccgc tctcgagggg atcgggcgcg ccgtggccga
     1981 ggagtccgtc gagctcgtcg gcgtgtggtc gcacttctcc tgggccgacg accccggcca
     2041 cccgacggtc cgccgccagc acgagaggtt cgtggacacg gttcgcacca tcgagtcagc
     2101 cgggctgcgt ccgcagatcc gtcatctggc caactccgct gcgacgctga ccaacccggc
     2161 tgtgcacttc gacctcgtcc ggccgggcct ggccgtctac ggcctctcgc cggtgcccca
     2221 gctcggcggg cccgagcact tcggcctggt cccggccatg acgctcgtgg cccagctgtc
     2281 gctcgtcaag cgggtgcctc gcggttcggg cgtgagctac gggcaccagt acgtcaccga
     2341 gcgggacacg gttctcgggc tcgtgccgat ggggtatgcc gacgggatcc cgcgcaatgc
     2401 caccaacgtc ggcccgctcg ccgtggcggg cgcccggcac accattgccg ggag
//
LOCUS       DATAIX010000289         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_215330, whole genome shotgun sequence.
ACCESSION   DATAIX010000289 DATAIX010000000
VERSION     DATAIX010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215330"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VHM65_04305"
     CDS             <1..691
                     /locus_tag="VHM65_04305"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX2704958.1"
                     /translation="WDWAITGGSLVAALLWGVGLTNIVWGGPVKVDPAVTGNFIFDGT
                     LLSLLGPVPLLGGLTFVGLFLTHGAMFISLKTDGEIRHEARALAVKTGLGTAVLAVAL
                     LLLVNLKHPSLVGFLGAGVAAAALLASLLMATKGREGWAFGGTFACIAFAVVSYFGAL
                     YPNAMRSSLGAANDLTLAAASSTPTTLTIMTWAAVVFTPVVLIYTAWTYWVFRKRISV
                     HHIPEPVAVTV"
     gene            742..>1783
                     /locus_tag="VHM65_04310"
     CDS             742..>1783
                     /locus_tag="VHM65_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX2704959.1"
                     /translation="MRPFDPRLLRVLPAARGAVAFLGLLGVLGGALAIGQAFAITRLV
                     VDLVARQPLQSAIVATALVLGGRMVATGLTEVVAARAGAQVSTALRERLLAHWLTLPA
                     ERRPDAARALTLATGGATSVEPYVARYLPTLVASVVLPGFAIVAMTLVDWLSALIVVV
                     TVPLLPLFAALIGRATHDATQRRWRTSAALAGHFLDVVRGLPTLVSYGRGRTQVGTIR
                     TVSERHRESTMATLKVAFLSSAALELLATLSVAIVAVSVGLRLAGGSMPLGTAMTAIL
                     LAPEAYWPIRRVGAEFHNAADGAEAIKAILDELDAPAGVPTRHVGADASVSGLTYSYP
                     GSDHPVIAGLDAA"
BASE COUNT          217 a          678 c          585 g          303 t
ORIGIN      
        1 ctgggactgg gccatcaccg gcgggtcctt ggtggcagcc ctcctgtggg gagtcggcct
       61 gaccaacatc gtgtggggcg ggccggtcaa ggtcgaccct gcggtcacgg ggaacttcat
      121 cttcgacggc accctgttga gcctgctcgg ccccgtgccg ctgctcggtg ggctgacctt
      181 cgtcggcctg ttcctcaccc acggcgcgat gttcatctcg ctcaagaccg acggcgagat
      241 ccggcacgag gcccgcgcgc tggccgtcaa gaccggcctc ggcaccgcgg tgctcgccgt
      301 ggcgctgctc ctgctcgtga acctcaagca cccgtcgctg gtcggtttcc tcggggccgg
      361 cgttgccgca gcggccctgc tcgcttcgct gttgatggcc accaagggcc gtgagggctg
      421 ggcgttcggc gggacgttcg cctgcatcgc cttcgcggtc gtgtcgtact tcggtgcgct
      481 ctaccccaac gccatgcggt cctcgctcgg cgccgccaac gacctcaccc tggcggccgc
      541 atcgagcacg ccgacgacgc tgacgatcat gacgtgggcg gcggtcgtct tcaccccggt
      601 cgtcctgatc tacacggcat ggacatactg ggtcttccgc aagcggatct cggtccacca
      661 catccccgag ccggtcgcgg tcaccgtctg agcaagaccc gagcgggcgg gccgcgtggc
      721 ccgacctctt aggctgagcc agtgagaccg ttcgacccac gactgctccg cgtgctccct
      781 gcagcacgcg gagcagtcgc gttcctcggc cttctcgggg tcctcggcgg agcgctggcc
      841 atcgggcagg cgttcgcgat cacccggctc gtcgtcgacc tggtcgcccg tcagcccctg
      901 cagagtgcca tcgtggccac cgcgctggtc ctcggcgggc ggatggtcgc gaccgggctg
      961 accgaggtcg tcgccgcccg cgcaggcgcg caggtctcga cggctctgcg cgagcggctg
     1021 ttggcgcact ggctcaccct gcccgccgaa cgtcgccccg acgcagccag ggccctcacc
     1081 ctcgcgaccg gcggcgccac cagtgtcgag ccgtatgtcg cgcgctacct cccgacgctg
     1141 gtcgcctcgg tcgtcctgcc gggcttcgcg atcgtcgcga tgacactggt cgactggctc
     1201 agcgctctca tcgtcgtggt caccgtgccg ctgctgccgc tcttcgccgc gctgatcggg
     1261 cgagccaccc acgatgccac gcagcggcgc tggcgcacga gtgccgcgtt ggccgggcac
     1321 ttcctcgatg tcgtccgagg gctaccgaca ctcgtctcct atgggcgcgg ccgcacccag
     1381 gtcggcacca tccgcaccgt cagcgagcgc catcgcgagt cgaccatggc caccctcaag
     1441 gtggccttcc tgtcctcggc ggccctcgag ctgctcgcca ccctctcggt cgcgatcgtc
     1501 gccgtgagtg tggggctgcg gctcgccggt ggctcgatgc ccctcggaac cgcgatgacg
     1561 gccatcctgc tcgcacccga ggcctactgg ccgatccgcc gggtcggtgc ggagttccac
     1621 aatgccgccg acggcgccga ggcgatcaag gccatcctcg acgagctcga cgcacccgcg
     1681 ggggtgccca cccggcatgt cggagccgac gcatccgtct cgggcctgac ctactcctac
     1741 ccaggaagcg accaccccgt cattgccggg ctcgatgcag cct
//
LOCUS       DATAIX010000290         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_165158, whole genome shotgun sequence.
ACCESSION   DATAIX010000290 DATAIX010000000
VERSION     DATAIX010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165158"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VHM65_04315"
     CDS             <1..465
                     /locus_tag="VHM65_04315"
                     /inference="COORDINATES: protein motif:HMM:NF021900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX2704960.1"
                     /translation="GSSIMPGKVNPVIAEATLMVCAQVIGYDATVTFAGASGSFELNV
                     MMPVMARSVLESIRLLTSSVVALADRCILGIEAKAEHCRDLAESSPAIVTPLARVVGY
                     ERAAAIAKRSVREGTTIRTAALDEGVVADGSLSADDLDRILDIGAMTRPRPG"
     gene            619..1338
                     /locus_tag="VHM65_04320"
                     /pseudo
     CDS             619..1338
                     /locus_tag="VHM65_04320"
                     /inference="COORDINATES: protein motif:HMM:NF025615.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
     gene            1335..>2131
                     /locus_tag="VHM65_04325"
     CDS             1335..>2131
                     /locus_tag="VHM65_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2704961.1"
                     /translation="MRAPLLVAVAVGAGRDALDLLGPLDAALRGVGPALLPHSIGEPA
                     PVIAEGGLADGPGDLALAVGTSGSTGSPKRALLTAANLAASATATYAALGGPGQWVLA
                     LPATHIAGLQVLVRSLLAGTTPVTLGSGDGFTAAGFAVAVNALSAGRRYASLVPTQVD
                     RLLRDAPGSAALAGLDAVLVGGAPAPPALLERAAAAGARVVRTYGMSETAGGCVYDGE
                     PLPCTRIRVEQGRILVGGATVAHGYLGDLAATAAAFTTDGHGQRWFHT"
BASE COUNT          224 a          810 c          781 g          316 t
ORIGIN      
        1 gggtcctcga tcatgcccgg caaggtcaac ccggtcatcg ccgaggcgac cctcatggtg
       61 tgcgcgcagg tcatcgggta tgacgcgacg gtcactttcg ccggggcgag cgggtccttc
      121 gagctcaacg tcatgatgcc ggtgatggcc cggtcggtgc tcgagtcgat ccggctgctc
      181 acgagctcgg ttgtcgccct ggccgatcgg tgcatcctcg ggatcgaggc caaggcggag
      241 cactgccggg acctggcgga gtcctcaccg gccatcgtca ccccattggc ccgggtcgtc
      301 ggctacgagc gggccgccgc gatcgccaag cgctcggtcc gcgagggaac gacgatccgg
      361 acggccgccc tcgacgaggg cgtcgtcgcc gacgggagcc tctctgcgga cgacctcgac
      421 cgcatcctcg acatcggtgc catgacccgc cctcggccgg gctgagccga cccccagatc
      481 cgccaccgtg cgcttcgcgc cgccgtcggc acagcctgct ccgacccgac cgcgccacgg
      541 agcccgacga ccacgatcta ccctggggag gtgagcgccc ccagccagcc ggccgcccgg
      601 cataccgggc ctgccagggt gcggcgggga cccggctggg tcccgacgca gcacggggcc
      661 tgggcgatgc tcgccagccc cctgctcgtc ggcatcctcg cgagccgccc ggcctgggtg
      721 cacctcccgc tggcggcgct ctggttcgtg gggtacttcg ccttcttcgc gacgagcctg
      781 tggctcaagg cccagcggcg cgccaagctc ctgccgccgg tgcgggtgta tggcgcggcc
      841 agccttgccc tcgcggcggt cgtcgcgctc gtcacgccag ggctggcgcg gtgagcgccg
      901 ttgttcgtcg cgccgctcgg gatcgggctg tgggctgcgg cccgcaggcg ggagcgggcg
      961 acgtggagtg ggctggcgac cgtggcgggg tcggcgctca tgacggtggt cgcctatgac
     1021 gcgggcggcg gcaccgaccc gcaccgcgcg tggctgctcg cggcagccca ggcggcctac
     1081 ttcgccggga cggtgttcta cgtcaagtcg ctgatccgcg agcgcggcaa cgtgtcgttc
     1141 ctgcgggcca gcatcgccgc ccacgtcgtg ctggctgcgg cgatgttcgc gctcagcccg
     1201 tggctcgggc tcgtcttcgg cctgctcgcg ctgcgcgcac tgctcgtccc gcggtggtcc
     1261 ccacgcccgg cgcaggtcgg tgcgggcgag atcgtcgcca cgctcgtggt cgccgtcgtc
     1321 tccctcgtcg tcacgtgagg gcgcccctgc tcgtcgccgt cgccgtcgga gccggtcgcg
     1381 acgcactcga cctgctgggt ccgctcgacg ccgcgctgcg cggagtgggc ccggccctgc
     1441 tcccccactc catcggcgaa cccgctcccg tcatcgccga gggggggctc gccgacggtc
     1501 caggcgacct tgcgctggcc gtcgggacgt cgggctcgac cgggtcgccc aagcgggcgt
     1561 tgctcacggc cgccaacctg gcggcatcgg ccacggcgac gtacgccgcc ctcggcggtc
     1621 ccgggcagtg ggtcctggcc ctcccggcga cgcacatcgc cgggctccag gtgctcgtcc
     1681 ggtccctgct cgccggcacc acaccggtca cgctcgggtc cggggacggg ttcacggccg
     1741 cgggctttgc cgtggccgtg aacgcgctca gcgccggccg ccggtacgcc tcgctcgtcc
     1801 cgactcaggt cgaccggctc ctgcgtgacg cgccggggtc cgcggcgctg gcaggcctgg
     1861 atgcggtcct cgtcggtggc gcgcccgccc cgccggcctt gctggagcgc gccgctgcgg
     1921 ccggggccag ggtcgtgcgc acctacggca tgagcgagac cgcgggcgga tgcgtgtatg
     1981 acggggagcc cctgccgtgc acccggatcc gggtcgagca gggccgcatt ctcgtcggcg
     2041 gcgccacggt ggcccacggc tatctcggcg acctggcagc gaccgcagcg gccttcacga
     2101 ccgacggcca cgggcagcgg tggttccaca c
//
LOCUS       DATAIX010000291         1857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_215375, whole genome shotgun sequence.
ACCESSION   DATAIX010000291 DATAIX010000000
VERSION     DATAIX010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215375"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VHM65_04330"
     CDS             <1..706
                     /locus_tag="VHM65_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX2704962.1"
                     /translation="LTDYLLERGVRVRYLHSEVDTLRRVELLRELRMGEYDVLVGINL
                     LREGLDLPEVSLVSILDADKEGFLRSARSLIQTIGRAARNVSGQVHMYADRVTPSMEA
                     AIEETRRRRVKQVAYNVAHGVDPQPLRKKIADITDLLQREDADTESLLGSGRNQSRGK
                     GGSGRAAMVADVAVASRSTTLAGMAANDLAGLIQELTTQMHHAAADLHFELAARLRDE
                     VADLKKELRQMTNATS"
     gene            800..1096
                     /locus_tag="VHM65_04335"
     CDS             800..1096
                     /locus_tag="VHM65_04335"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEX2704963.1"
                     /translation="MRDCAPVLSLVDWPIGRPAVLVAVDGDPAFARRVSQLGLRPDTV
                     LTPVLQTVGGGRVVAAGALRLALDHASAAMLRVEETSPPAEADPSSDAGTMRPT"
     gene            1093..>1857
                     /locus_tag="VHM65_04340"
     CDS             1093..>1857
                     /locus_tag="VHM65_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HEX2704964.1"
                     /translation="MSDAPMTVAPAVASCHEPGAGAVAPSGSPVVALAGAPNVGKSTL
                     FNALTGARRRMGNWPGTTVEVGRGLWRAAGTPAPGARPSAYDLIDLPGAYSLDPTSPD
                     EALTRALLVDVPPADRPALVVVVADSTHLARSLYLVAELRERDRRVVVALTMVDIAAR
                     RGIVVDVDRLSAALGVPVVPVDPRRRTGTAELGVAVTRALTGPAPRARPHPGTGDTFE
                     LADERFAWVDAAVGPSVTTAAHVRRTWSDRIDRAVLH"
BASE COUNT          258 a          695 c          646 g          258 t
ORIGIN      
        1 cctgaccgac tacctcctgg agcgcggtgt ccgggtgcga tacctccact ccgaggtgga
       61 cacgctgcgc cgcgtcgagc tgctgcgtga gctgcggatg ggggagtacg acgtcctggt
      121 cggcatcaac ctgctccgcg agggcctcga cctgccggag gtgtcgctcg tcagcatcct
      181 cgacgccgac aaggagggat tcctgcgctc ggcgcgcagc ctgatccaga cgatcggccg
      241 ggcggcgcgc aacgtgtccg gacaggtcca tatgtatgcc gacagggtga ccccctcgat
      301 ggaggcggcg atcgaggaga cccggcggcg gcgcgtcaag caggtcgcct acaacgtggc
      361 ccacggggtc gacccgcagc cgctgcgcaa gaagatcgcc gacatcaccg atctgctcca
      421 gcgcgaggac gccgacaccg agtcgctgct cggcagcggt cgcaaccagt cgcggggcaa
      481 gggcgggtca gggcgtgcag cgatggtggc cgatgtcgcg gtggcctcgc ggagcacgac
      541 cctcgcgggt atggcggcca acgacctcgc cggcctcatc caggagctga cgacccagat
      601 gcaccacgcg gcagccgacc tgcacttcga gctggcggcg cggctccggg acgaggtcgc
      661 ggacctcaag aaggagctgc gtcagatgac caacgcgacc tcgtgagacg cccggcgacg
      721 tggtactgag acggaagcca cgtgccgcga tgcagtacac gacttaggta aacctaacct
      781 gacgccctag gatggcgtca tgcgcgactg cgccccggtt ctctcgctgg tcgactggcc
      841 catcggccgc ccagccgtcc tcgtcgccgt cgacggcgat cctgccttcg cccgccgggt
      901 ctcccagctc ggcctgcggc cggacaccgt cctgaccccg gtgctgcaga ccgtgggggg
      961 cggccgggtc gtggccgccg gtgccctgcg gctggccctg gaccatgcct ccgccgcgat
     1021 gctgcgcgtc gaggagacct cgcccccagc cgaggccgac ccctcatctg atgccggcac
     1081 gatgcggccg acatgagcga cgccccgatg acggtggccc ctgcggtcgc gtcctgtcac
     1141 gagcccgggg cgggagcggt cgcgccatcg gggagcccgg tggtggccct ggccggcgcc
     1201 ccgaacgtcg gcaagtcgac cctgttcaac gcactcaccg gcgcccggcg caggatggga
     1261 aactggcccg gcacgaccgt cgaggtcggc cgtgggctct ggcgggcggc cggtacacct
     1321 gcgcccgggg cgaggccttc ggcatacgac ctcatcgacc tcccgggtgc ctactcactg
     1381 gacccgacgt cccccgacga ggcgctgacc cgcgcgctcc tcgtcgatgt accccccgct
     1441 gaccggcccg ctctcgtcgt cgtcgtggcc gactcgaccc acctggcccg cagtctctac
     1501 ctcgtcgccg agctgcgtga acgtgaccgg cgcgtggtcg tcgcgctgac gatggtcgac
     1561 atcgcggcgc gtcgcggcat cgtcgtcgat gtcgaccggc tcagcgccgc actcggcgtc
     1621 cccgtcgtgc cagtggatcc gcggcgccgc accggcacgg cagagctcgg cgtggcggtt
     1681 acgcgcgcgc tgaccggccc agcgccccgc gcgcgccccc atccgggcac cggcgacacc
     1741 ttcgagctcg ccgacgagcg cttcgcctgg gtcgatgccg ccgtcgggcc ctcggtcacg
     1801 accgcggctc acgtccggcg cacctggagc gaccggatcg accgggccgt cctgcac
//
LOCUS       DATAIX010000292         1727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_126046, whole genome shotgun sequence.
ACCESSION   DATAIX010000292 DATAIX010000000
VERSION     DATAIX010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126046"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1587)
                     /locus_tag="VHM65_04345"
     CDS             complement(22..1587)
                     /locus_tag="VHM65_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX2704965.1"
                     /translation="MSAADGESRLELQDLERLAISAHLVGRTDDGVGAWERAHRACVQ
                     EGDATRAARCAFWLAFVLLNRGEFARGGGWVHRGQRLLDQAEIDCVEHGYLRYGASLR
                     LVLEGDPGAAAVGFAEAAQIGDRFRSMELVALARVGHGRCLIRSGDTAGGMALLDEAM
                     AAITTAEVSATAIGDLYCTVIEGCQEVFDVRRAQEWTEALNRWCETQPDLVLYHGQCL
                     VHRAELMLLAGLWPAALAEAHRACARLSRPRSQPALGAAHYVRAELHRLRGELAEAEN
                     GYGQASALGREPQPGLAQLRLAQGRTGEAVTALRRAIEAAADDPATRAKLWGPMVETE
                     LAAGDVASARAASDELSSLASACDTPYLNAVSALALGSVLLAEGDIGASLAALRRAWA
                     GWTELEAPHEAARARVLIGLAFRAMCDEESAARELSAARSALERLGAPSDLARAAEQR
                     QAMPRVDGAGLTPRELEVMRLLATGTTNRAIAAALFISEKTVATHVSSILRKLCLPSR
                     AAATAYAHQRRLL"
BASE COUNT          283 a          618 c          576 g          250 t
ORIGIN      
        1 ggggactgcg tgaatgagct cctagaggag gcgccgttgg tgggcatacg cggtcgctgc
       61 tgcgcgagaa ggcaaacaca gtttgcgcag gatgctgctg acatgtgtgg ccacggtctt
      121 ctcgctgatg aagagagcgg cggcgatggc tcggttggtg gtgccggttg ccaacagccg
      181 catcacttct agctcgcgag gtgtcagacc ggcaccatcg acccgtggca tcgcctggcg
      241 ctgttctgca gcgcgcgcaa ggtcggatgg cgccccgagg cgctcgagcg cggatcgggc
      301 ggcacttagc tccctcgccg cgctctcctc gtcgcacatg gcccggaagg cgagaccgat
      361 cagcactcgg gcccgtgccg cctcgtgggg tgcctccagc tcggtccagc ctgcccaggc
      421 ccggcggagc gcggccaatg aggcgccgat gtctccctcg gccaggagca ccgatccaag
      481 cgcgagcgcc gagacggcat tgagataggg ggtgtcgcat gcggaggcga gactcgacag
      541 ctcgtcagaa gcggcccgag cagaagcgac atcaccggcg gccagctcag tctcgaccat
      601 gggcccccag agcttcgctc tggtggccgg gtcgtccgcg gccgcctcga tggcccggcg
      661 cagagcagtc acagcctcgc cagtccgacc ctgcgcgaga cggagctggg ccaggccggg
      721 ttgtggctcg cgccccagag cactcgcctg cccgtacccg ttctccgcct cggcgagctc
      781 cccgcgcaag cggtggagct cggcccggac gtagtgcgca gcaccgagag cgggttggct
      841 ccgaggtcgg gacagcctgg cgcaggcccg gtgtgcctcg gccaaggcag ccggccacaa
      901 cccggccagc agcatgagct cggctcgatg gaccaggcat tggccgtggt agaggacgag
      961 gtcgggctga gtctcgcacc aacggttgag cgcctcagtc cactcctggg ctcgccgcac
     1021 gtcgaacacc tcttggcagc cctcgatcac ggtgcagtac aggtcaccta tcgccgtcgc
     1081 ggagacctcg gccgtcgtga tggcggccat agcctcgtcg agcagcgcca ttcccccggc
     1141 agtgtccccg ctgcggatga ggcaccgacc gtggcccacg cgggccagtg ccaccagctc
     1201 catgcttcgg aaacggtcgc cgatctgggc ggcctccgca aaaccgacgg cggcggcccc
     1261 ggggtcgccc tccagcacca agcgcagaga ggcgccgtac cgcagatagc cgtgctcgac
     1321 gcagtcgatc tccgcctggt cgagcagccg ctgcccacgg tggacccaac ctccgccgcg
     1381 ggcaaattca ccgcggttga gcaggacgaa cgccaaccag aacgcgcagc gggcggcacg
     1441 ggtcgcatcg ccttcctgca cacacgctcg gtgggcacgt tcccacgccc cgacgccgtc
     1501 gtcggtccgg cccaccaggt gcgcactgat cgcgagccgc tccagatcct gcaactcgag
     1561 gcgcgactcg ccgtcggccg ccgacaaatc cttgagggca tcggcccatg cctgacgttc
     1621 gaatgccttc cgaccccgat cgagctgacc agccataacg ccaagtgtcg tcctctcagc
     1681 ttgcgattaa accccgctat cccgcgacca agaaggccgc atagcgg
//
LOCUS       DATAIX010000293         1805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_105498, whole genome shotgun sequence.
ACCESSION   DATAIX010000293 DATAIX010000000
VERSION     DATAIX010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105498"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VHM65_04350"
     CDS             complement(<1..191)
                     /locus_tag="VHM65_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX2704966.1"
                     /translation="MKYLIVVLVIIVLLVLLLVGMYNGLIRLRNLVHEAWAQIDTELK
                     RRHDLIGNLVETVKGYAQH"
     gene            complement(250..801)
                     /gene="pyrE"
                     /locus_tag="VHM65_04355"
     CDS             complement(250..801)
                     /gene="pyrE"
                     /locus_tag="VHM65_04355"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX2704967.1"
                     /translation="MTGTDRDRLLRLIQDIAIVHGRVTLSSGKEAGYYVDLRRITLHG
                     EAAPLVGRVMRTLTADLAYDAVGGLTLGADPVATSMLHTAAAAGERLDAFVVRKAGKA
                     HGMQQRIEGPSITGRRVLVVEDTTTTGASPLAAVEAARAAGALVVAVATIADRATGAG
                     ERIRAEAGVDYRYAYGLAELGLG"
     gene            complement(812..1594)
                     /locus_tag="VHM65_04360"
     CDS             complement(812..1594)
                     /locus_tag="VHM65_04360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2704968.1"
                     /translation="MSTALVTGGTSGIGLEFARQLAERGHGIVLVARDRARLENVSDE
                     LTRAYGVPTEILVADLGDRAQLAKVADRLGDPRRPVDLLVNNAGFALRTSFLRGDLAD
                     EEALFDVLCRAVLVLSHAGARAMVERGRGGIINVSSVASFVSMGSYSAAKAWVTVFSE
                     GLAVELTGTGVSVTVLCPGFTHTEFHKRAGLSMSRLPQILWLDAPRLVRDCLDDAAKG
                     KVVSVPGAPYKVLVWALRLMPRSLARTVSGGVGRRRRPPSGR"
     gene            1615..>1805
                     /locus_tag="VHM65_04365"
     CDS             1615..>1805
                     /locus_tag="VHM65_04365"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX2704969.1"
                     /translation="MSGGRPVASEAGPRVGGVRIATWNVNSIRSRVDRVAAWLERTDV
                     DVLAIQETKAREDQFPYLP"
BASE COUNT          272 a          642 c          651 g          240 t
ORIGIN      
        1 tcgtgctgcg catagccctt gaccgtctcg accaggttgc cgatgagatc gtggcgacgc
       61 ttgagctcgg tgtcgatctg ggcccaggcc tcgtggacga ggttgcgcag ccggatcagc
      121 ccgttgtaca tcccgacgag caacagcacg agcagcacga tgatgacgag cacgacgatg
      181 agatacttca cggaccctcc cggagtcgct gggtagaccg atcctatgat gctcagctcc
      241 tgtgatggct cagcccagcc cgagctcggc cagcccgtag gcgtagcggt agtccacacc
      301 cgcctcggcc ctgatccgct cccccgcgcc ggtcgcccgg tcggcaatcg tggcgacggc
      361 caccaccagg gcgccggccg cgcgggcggc ctcgacggcg gccaggggtg aggctccggt
      421 ggtcgtcgtg tcctcgacga ccaggacacg acgccccgtg atcgaggggc cctcgatgcg
      481 ctgctgcatc ccatgggcct tgccagcctt gcgcacgacg aacgcgtcga gccgctcgcc
      541 cgccgcagcg gcggtgtgca gcatcgaggt cgccacgggg tcggccccga gcgtgagccc
      601 gccgacggcg tcataggcga ggtccgcggt gagcgtccgc atgacccggc cgacgagcgg
      661 ggcggcttcg ccgtgcagcg tgatccggcg caggtcgacg tagtagccgg cctccttgcc
      721 cgaggacagc gtgacccggc cgtggacgat cgcgatgtcc tggatgagcc tgagcagccg
      781 gtcgcggtcg gtcccagtca tgggggcaac cctaacgacc cgagggcggg cggcggcggc
      841 ggcccacacc cccggacacc gtccgggcga ggctgcgcgg catgagccgc agggcccaga
      901 cgagcacctt gtagggcgca cccgggacgc tgaccacctt gcccttcgcc gcgtcgtcga
      961 ggcagtcgcg gacgagccgg ggggcatcga gccagaggat ctgcggcagc cggctcatgg
     1021 agaggccggc ccgcttgtgg aactcggtgt gcgtgaagcc gggacacagg acggtcaccg
     1081 acaccccggt gccggtgagc tcgaccgcca gcccttcgga gaagacggtg acccaggcct
     1141 tggccgccga gtaggacccc atcgacacga acgaggccac cgacgacacg ttgatgatgc
     1201 ccccgcggcc gcgctccacc atggcgcgcg cgcccgcatg gctcaggacg agcaccgccc
     1261 ggcacagcac gtcgaacagc gcctcctcgt cggccaggtc gccgcgcagg aagctcgtcc
     1321 gcagggcgaa cccggcgttg ttgacgagca ggtcgaccgg ccgccggggg tccccgagcc
     1381 ggtcggcgac cttggccagc tgggcccggt cgccgaggtc cgcgacgagg atctccgtcg
     1441 gcaccccata cgcccgggtg agctcgtccg agacgttctc gagccgggcg cggtcgcgcg
     1501 cgacgaggac gatgccgtgc ccgcgctcgg cgagctggcg ggcgaactcc aggccgatgc
     1561 ccgaggtccc ccccgtgacg agtgcggtgc tcatgggggt gagcataggc cggtatgtcg
     1621 ggcggccggc cggttgcgtc cgaggcggga cctagggtgg gaggcgtgcg aatcgccacg
     1681 tggaacgtca actccatccg ctcgcgcgtc gaccgggtcg ccgcctggct cgagcgcacc
     1741 gacgtcgacg tgctcgcgat ccaggagacc aaggcccgcg aggaccagtt cccgtacctg
     1801 ccgtt
//
LOCUS       DATAIX010000294         3130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_75955, whole genome shotgun sequence.
ACCESSION   DATAIX010000294 DATAIX010000000
VERSION     DATAIX010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75955"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /gene="arfB"
                     /locus_tag="VHM65_04370"
     CDS             <1..354
                     /gene="arfB"
                     /locus_tag="VHM65_04370"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX2704970.1"
                     /translation="VNTADSRVQLTVDIAGSPSLTEHLRRRLLARLAPRLIDGELTVA
                     ASEHRTQLANRRAARDRMAHLLREAAAPPPPARRPTKATASSRERRIASKKRRGVIKK
                     GRGHPEPPDTPPPRG"
     gene            complement(368..1591)
                     /locus_tag="VHM65_04375"
     CDS             complement(368..1591)
                     /locus_tag="VHM65_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2704971.1"
                     /translation="MDPIRNPYAPGAGQRPPELAGRAEQLAAFDVVLERVSRGRPERS
                     IVLVGLRGVGKTVLLNAMRSAAVRAGWGTGKLEARPEQRLRRPLSAALHQALRELGHP
                     SADGVEHVLGVVKTFAQREVGPRARLRDRWQPGIDAPAVAGRADSGDIEIDLVELLTD
                     VGGLAGDTGRGVAIFIDEMQDLGTDDVSALCAAAHEISQARLPVIVVGAGLPHLPSLL
                     SASKSYSERLFRYSRIDRLDVEAASLALSAPAREEGAQWSADALAAMYDATAGYPYFI
                     QAYGKAVWDQAPGSPITVQDVRAGAPEAEAELGVGFFGSRYERATPAEREYLRAMADA
                     AGGDGPAYRSSLSVELDSVPTALVATVLERKPQSLSPARDALLKKGLIYSAERGRIAF
                     TVPHFGRYLRSHSPE"
     gene            complement(1729..2832)
                     /locus_tag="VHM65_04380"
     CDS             complement(1729..2832)
                     /locus_tag="VHM65_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704972.1"
                     /translation="MPIVFVHGVAMRDPDDPQYEVFNRLYRGIDWPLIESLLREHVAP
                     VLRPHAPEDVSIDWVYWGDLGTHPPEPVLGPAVNLDGLGEIASLTADELGEALQHTLF
                     RALPPAHWAAAVRAVWAVVGQGSLRAQLATRSPKRQRAFLDKLVHDRLIAEAPELGVR
                     LSPIVIDLAALRRRNVRWAMSGVRGPYEAFVPVFVGDVLRYLAGRGRPGEPGEVIVRV
                     VRALTAAAAAGPPGEPFVVLTHSMGGQLVYETLTAFAPGLHVDFWCASGGQVGLFRQL
                     GVFLEATSADIAPLDTARVGYFWNAWSSSDVLSFPAEGWVPGAHDSDFAFTGGLPHNH
                     LAYLRDPDFYRTLAAKVLVHSPAGIHPPRHTRV"
BASE COUNT          487 a         1171 c         1079 g          393 t
ORIGIN      
        1 gtcaacacgg ccgactcgcg tgtccagctc accgtcgaca tcgccggctc tccctcgctg
       61 accgagcacc tgcgccgccg actgctcgct cggctcgccc ctcggctgat cgacggcgag
      121 ctgaccgtcg cggccagcga acaccgcacc cagctggcca accggcgcgc cgcgcgtgac
      181 cgaatggctc acctacttcg tgaagccgca gcgccgccac caccggcgcg ccgccccacc
      241 aaggcgacgg cgagctcacg cgagcgccgc atcgcgtcca agaaacggcg cggtgtgatc
      301 aagaaggggc gcggccaccc cgagccgccc gacacgccac caccccgggg gtgacgtccg
      361 aaccgagcta ttcgggcgag tgggagcgca ggtagcggcc gaagtggggg acggtgaagg
      421 cgatccggcc gcgctcggcg ctgtagatca gccccttctt gagcagcgca tcgcgtgccg
      481 gtgacagcga ctgcggcttg cgttcgagca ccgtggccac cagggccgtc ggcaccgagt
      541 ccagctcgac cgacagcgac gaccggtatg ccggaccgtc gcctcccgcc gcgtcggcca
      601 tggcccgcag gtactcgcgc tcggccgggg tggcccgctc gtagcgggac ccgaagaacc
      661 ccaccccgag ctcggcctcg gcctcgggcg ccccggccct cacgtcctgc accgtgatcg
      721 gcgaccccgg tgcctggtcc cacaccgcct tgccgtacgc ctggatgaag tacggatacc
      781 cggcggtcgc gtcatacatc gccgcgaggg cgtcggcact ccactgggct ccctcctcac
      841 gagccggcgc cgacagcgcc aacgaggccg cctcgacgtc cagccggtcg atgcgtgagt
      901 agcggaacag cctctccgag tatgacttcg acgccgagag caacgacggc agatgcggca
      961 gcccggcccc gacgacgatg accggcagcc gcgcctggct gatctcgtgc gccgccgcgc
     1021 acagcgccga cacatcgtcc gtcccgaggt cctgcatctc gtcgatgaag atcgccaccc
     1081 cccggccggt gtcccccgcc agcccgccga cgtcggtcag cagctcgacg aggtcgatct
     1141 cgatgtcacc ggagtcggcg cgccccgcca cggcaggcgc gtcgatcccc ggttgccagc
     1201 ggtcgcgcag ccgcgccctc ggcccgacct cgcgctgggc gaacgtcttg accacgccga
     1261 gcacatgctc gacgccgtca gccgaggggt gaccgagctc gcgcagcgcc tggtgcaggg
     1321 cggcggacag cggccgccgc agccgttgct cgggccgcgc ctcgagcttg ccggtccccc
     1381 acccggcacg cacggccgcc gagcgcatcg cgttgagcag gacggtcttg ccgacgccgc
     1441 gcagcccgac gagcacgatc gagcgctccg gccggccgcg cgagacgcgc tcgagcacga
     1501 cgtcgaaggc ggccagctgc tcggcgcgcc cggccagctc gggcggacgc tgcccagcac
     1561 cgggcgcata cgggttgcgg atcgggtcca tgatcggact gtatgccggg atctagcgct
     1621 cgcccgagat tgcggcatac accggtatga cgtgtcgccc ccgcccccgc acccggacgg
     1681 tccacgatcg ggctgtattc cggactctag cgccagcccg atacttcgtc atacacgggt
     1741 atgacgtggt gggtggatcc ctgctgggct gtggacgagg accttcgcgg cgagggtccg
     1801 gtagaagtcg gggtcgcgca ggtaggcgag atggttatgg ggcagcccgc cggtgaaggc
     1861 gaagtccgag tcgtgcgccc ccggcaccca cccctccgcg gggaagctga ggacatcgct
     1921 cgagctccag gcgttccaga agtagccgac ccgcgcggtg tcgagcggcg cgatgtccgc
     1981 agaggttgcc tcgaggaaga ctccgagctg acggaagagc ccgacctgtc cgcctgacgc
     2041 gcaccagaag tcgacatgga ggccgggcgc gaaggcggtg agggtctcgt agacgagctg
     2101 accgcccatg ctgtgggtca ggaccacgaa cggctcccct ggcggacccg ctgccgccgc
     2161 cgcagtgagc gcgcggacca cgcgcacgat gacctcgccc ggttcccccg gccggcctcg
     2221 cccggcgaga taacgcagca catccccgac gaagaccggc acgaacgcct cgtacggccc
     2281 ccgcacgcca ctcatcgccc atcgcacgtt tcgccgccgc agcgccgcca ggtcgatgac
     2341 gatcgggctg agccgcaccc ccagctcggg cgcttcggcg atgaggcggt catggacgag
     2401 cttgtccagg aacgcccgct gccgcttcgg cgagcgcgtt gccagctggg ccctcagcga
     2461 gccctgcccg acaaccgccc agactgcgcg cacggcggca gcccagtggg ccggcggaag
     2521 cgcccggaac agggtgtgct gcagcgcctc cccgagctcg tccgcagtga gtgaggcgat
     2581 ctcgccgagg ccgtcgaggt tcaccgccgg cccgagtacg ggctccggcg ggtgcgtccc
     2641 gaggtcaccc cagtagaccc agtcgatcga gacgtcctcg ggcgcgtggg gccgtagcac
     2701 cggcgcgacg tgctcgcgca gcagcgactc gatgaggggc cagtcgatgc cgcgatagag
     2761 ccggttgaag acctcgtact gcgggtcgtc cgggtcgcgc atggcgacac cgtggacgaa
     2821 gacgatcggc accactggag tgtatgccgg gctggagtgt atgccgggcg ccggagcggg
     2881 cgtcgccagc acgcggtggc ccggccggcc gactgtgtcg ctgtcgctca ctatgccgtg
     2941 cccgccagga gcgggcacac gcggtagcga cggcgcacgc cccacagcca ccatgccccg
     3001 cccgccagca gcgggcacgg catagtctcc ggaacgcaca tacgatgccg ccgcgggaac
     3061 gcctgcaccg ggctgacgca cggctaatgc atgggccaac gcagggctac cgtatgggca
     3121 cggcgtcgaa
//
LOCUS       DATAIX010000295         1772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_75993, whole genome shotgun sequence.
ACCESSION   DATAIX010000295 DATAIX010000000
VERSION     DATAIX010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75993"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VHM65_04385"
     CDS             <1..260
                     /locus_tag="VHM65_04385"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEX2704973.1"
                     /translation="LVPLVVYLVYKDRSPFVRQHASEALNFQITACIGYVVSVILVFV
                     IIGIFTFAAIALCAVIFAILGTVAANDGRPYRYPVNIRFVS"
     gene            complement(253..1476)
                     /gene="hemW"
                     /locus_tag="VHM65_04390"
     CDS             complement(253..1476)
                     /gene="hemW"
                     /locus_tag="VHM65_04390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX2704974.1"
                     /translation="MPSALPDGDPAPGDGRLPDSALDRLDGSDWGVYVHVPFCSVRCG
                     YCDFTTYTVPELGGRDALDGYPGLVERELDLARAVLPPAMGPVATVFLGGGTPTMLAP
                     AALGQILRAVDERFGLAPGAEVTTEANPDSVTARSLQELAAAGFTRVSLGMQSAVPRV
                     LATLERTHDPRNVAAAVAGARAAGLGVSLDLIYGTPGESHSDWEQSLDAALALEPDHV
                     SAYALVVEEGTRLAAAVRRGLVAAPEDDDEADKYELADSRLTAVGLAWYEVSNWAKSP
                     AHRCRHNEGYWAGASWWGIGPGAHSHVGGVRWWNVKHPRAYGDRLAAGVSPAAGRETL
                     TSEQAYDEWVLLGIRRSQGLPIDGLRPGGRIAVAGLVADGLLDAEALGAGRAALTRRG
                     RLLADAVVRRLVAPS"
     gene            complement(1477..>1772)
                     /locus_tag="VHM65_04395"
     CDS             complement(1477..>1772)
                     /locus_tag="VHM65_04395"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX2704975.1"
                     /translation="LDLRGARTGDRWRIGAPADPAHVVVEVTTPRIPCATFARWLGEP
                     SWVKRFAAYGESGCYLRVLRAGRLSAGAPIEVLVRGTGPTMGEQLATEMNLGA"
BASE COUNT          238 a          657 c          642 g          235 t
ORIGIN      
        1 tcctcgtccc gctcgtcgtc tacctcgtct acaaggaccg cagcccgttc gtgcgccagc
       61 acgcgtccga ggcgctgaac ttccagatca ccgcctgcat cgggtatgtc gtgtcggtga
      121 tcctggtctt cgtgatcatc ggcatcttca ccttcgccgc gattgccctg tgcgccgtca
      181 tcttcgccat cctcggcacg gtggccgcaa acgacggacg gccctaccgc taccccgtca
      241 acatccggtt cgtcagctag gcgcgaccag cctgcgcacg accgcgtcgg caagcagccg
      301 gccgcgccgg gtgagggcgg ctcggccggc gcccagggcc tccgcgtcga gcagcccatc
      361 ggcgacgagc ccggccacgg cgatgcgccc cccggggcgc aggccgtcga tggggaggcc
      421 ctgactccgc cggatgccga ggagcaccca ctcgtcatac gcctgctcgc tcgtgagcgt
      481 ctcgcggccc gcggccggag agaccccggc agccagccgg tcgccatacg cgcgcgggtg
      541 cttgacattc caccagcgca ctccgccgac gtggctatgt gctccgggac cgatgcccca
      601 ccagctcgct cccgcccagt aaccctcgtt gtggcggcac cggtgggcgg gcgacttcgc
      661 ccagttgctg acctcgtacc acgccaggcc gacggccgtg agccgggagt cggcgagctc
      721 gtacttgtcg gcctcgtcgt cgtcctcggg ggccgcaacc agcccgcgcc ggaccgcggc
      781 cgcgagccgg gtcccctcct cgaccaccag cgcgtaggcc gagacgtggt cgggttccag
      841 cgcgagtgca gcgtcgaggg actgctccca gtctgagtgg gactcccctg gcgtgccgta
      901 gatgaggtcg aggctgacac ccagccccgc ggcccgggcc cccgcgacag cagcggcgac
      961 gttgcgcggg tcatgggtgc gctccagggt ggcgagcacg cggggcaccg ccgactgcat
     1021 cccgaggctg acccgggtga agccggcggc cgccagctcc tgcagcgacc gcgcggtgac
     1081 cgagtcgggg ttggcctccg tcgtgacctc ggcgccgggt gccagcccga accgctcgtc
     1141 gacggcccgc aggatctgcc ccagggcggc cggcgcgagc atcgtcggcg tgcctccgcc
     1201 gaggaacacc gtggcgaccg gtcccatggc cggggggagg acggctctgg ccaggtcgag
     1261 ctcgcgctca acgaggccgg ggtacccgtc gagcgcgtcg cgcccgccga gctcgggcac
     1321 ggtgtacgtg gtgaagtcgc agtagccaca gcgcaccgag cagaacggca cgtgaacgta
     1381 gacaccccag tcggagccgt cgagccggtc cagcgccgag tccgggagcc ggccgtcgcc
     1441 gggcgccggg tcgccgtcgg ggagggcgga gggcatctag gcgccgaggt tcatctcggt
     1501 ggcgagctgc tcacccatcg tggggccggt tccccggacg agaacctcga tcggggctcc
     1561 agcgctgagc cggcccgcgc ggagcacgcg caggtagcag ccggactcgc catacgccgc
     1621 gaagcgcttg acccacgacg gctcgcccag ccagcgggcg aaggtcgcgc aggggatgcg
     1681 gggggtggtc acctcgacga cgacgtgggc cgggtcggcg ggggcgccga tgcgccaccg
     1741 gtcgccggtg cgggcgccgc gcaggtcgag tc
//
LOCUS       DATAIX010000296         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_105612, whole genome shotgun sequence.
ACCESSION   DATAIX010000296 DATAIX010000000
VERSION     DATAIX010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105612"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="VHM65_04400"
     CDS             <1..1028
                     /locus_tag="VHM65_04400"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX2704976.1"
                     /translation="SYAACSPGLTVAAVGTAAGGQTVARDLIKVDPVPGTPLATTLDV
                     ATQAAADTALSAVSGTASLVAIRVSTGQILAAANAPASAVVAMATTGHAAPGSSFKVV
                     SSLALLRAGLTPSSPIPCTRTITVDGRTFRNHSDYPPGGLGRIPLRTAVANSCNTAFI
                     SQQARVSQARLAEAGAALGLGPGDDLDLGFPAYLGSVPAEATGTEHAASLIGQGRVEA
                     SPLAMATVMASVMRGQTVRPVLLPGQARSHPAPAVPLTAAEAAALRTLLGAVVSQGSG
                     RALAPLGVTLAKTGTAEFGSGTPPKTHGWMVAGRGDIAIAAYVENGASGAKTAGPIIA
                     AFLTALG"
     gene            1169..1588
                     /locus_tag="VHM65_04405"
     CDS             1169..1588
                     /locus_tag="VHM65_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX2704977.1"
                     /translation="MTDTAQSEVAAEIDSSVVHVSTVVPHPLDHVWRVINSPQGVQTL
                     LGAGAKLGGKGEPWRAEDGSHGVLRSYHPLEQIRVSWHPDGDGPVSLVDLHVVSEQGQ
                     TRVDLRHEHLTKAGLDIQVLATRWTEALERLSVLTSG"
BASE COUNT          234 a          658 c          561 g          213 t
ORIGIN      
        1 cgtcatacgc ggcctgcagc cccgggctca ccgtcgcggc cgtcggcacc gccgcgggcg
       61 gccagaccgt cgcccgcgac ctcatcaagg tcgaccccgt ccctggcaca ccactcgcga
      121 ccaccctcga cgtcgcgact caggccgccg ccgacacggc gctgtccgcc gtgtcgggca
      181 ccgcgtcgct ggtcgcgatc agggtctcga cggggcagat cctcgccgcc gccaacgcgc
      241 cggccagcgc cgtggtcgcc atggcgacca ccggccacgc cgcccccggt tcgtcgttca
      301 aggtggtctc cagcctggcg ctgctgcgcg cgggcctcac cccgagctcg ccgatcccgt
      361 gcacccggac gatcacggtc gacgggcgga ccttcaggaa ccacagcgac taccccccgg
      421 gcggcctcgg gcggatcccc ctgcgcacgg ccgtggccaa ctcgtgcaac accgcgttca
      481 tctcccagca ggccagggtc tcccaggccc ggctcgccga ggccggcgcc gccctcggcc
      541 tcggcccggg cgacgacctc gacctgggct tcccggccta cctcggctcg gtcccggccg
      601 aggccaccgg caccgagcac gcggcctcgc tcatcggtca gggcagggtc gaggcctcgc
      661 cgctggcgat ggcgacggtc atggcgtcgg tgatgcgggg ccagacggtg cgcccggtcc
      721 tcctgccggg acaggcacgg agccatcccg cccctgccgt gccactcacg gccgccgagg
      781 ccgccgccct gcgcaccctg ctcggggcgg tcgtcagcca gggcagcggc cgggcgctgg
      841 cgccgctcgg ggtcacgctg gccaagaccg ggacggccga gttcggctcc gggacaccgc
      901 ccaagacgca cggctggatg gtcgccggtc gcggggatat cgccatcgcc gcgtatgtcg
      961 agaacggcgc ctcgggcgcg aagacggccg gcccgatcat cgcggccttc ctcacggcgc
     1021 tcgggtgacc gcccgccccc gcccgggcag tttcgccgag atcaccaggc caaccgcttg
     1081 cgtgcgagcg ccggacttgg tgtgctgaga ccctgaacac catcgcccgg tgagcagcgc
     1141 ccgccgggcc cccagcaagg agcctgagat gacagacacg gcacagtccg aggtggcggc
     1201 ggagattgac tcgtcggtgg tgcacgtcag cactgtcgtg ccccacccac tcgatcatgt
     1261 atggcgtgtc atcaacagcc cccagggcgt ccagaccctg ctcggcgcgg gcgcgaagct
     1321 cggtggcaag ggcgagccgt ggcgtgccga ggacgggagc cacggcgtcc tgcgcagcta
     1381 ccacccgctc gagcagatcc gggtctcctg gcatcccgac ggcgacggcc ccgtgtccct
     1441 cgtcgacctg cacgtcgtct ccgagcaggg ccagacccgc gtcgacctgc gccacgaaca
     1501 cctcacgaag gccggcctcg acatccaggt gctggccacc cgatggaccg aggcgctcga
     1561 acggctcagt gtcctgacct ccgggtagac ccggcctctc ggcagttgac cggccgattc
     1621 ccgccgaggt catgaatgag taacaaacct gctgcggcgc aggaac
//
LOCUS       DATAIX010000297         4030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_126132, whole genome shotgun sequence.
ACCESSION   DATAIX010000297 DATAIX010000000
VERSION     DATAIX010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126132"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VHM65_04410"
     CDS             <1..1173
                     /locus_tag="VHM65_04410"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX2704978.1"
                     /translation="VQLVAADGYFAALGSRAAAGAADLRDVTRAALTDAAAQAAGAYA
                     EMATFLRDELQGASPEADACGRERYTLVSQYFLGAVVDLEETYQWGQEELARITAEMA
                     QVADQIKPGASVVEAIEVLNADPAYLLQGTDALKEWMQAKADEAIANLSGTHFDIPEP
                     VRRIECMIAPTQTGGIYYTGPSEDFSRPGRMWWSVPRGVTDFRTWNELTTVYHEGVPG
                     HHLQVAQTTFRSDLLNKWRRLDSWTSGYGEGWALYAERLMADLGYLDDPGARMGMLDG
                     QSMRAARVVLDIGIHCGFDAPAEVGGGAWTYDKAWAFLTGHAFGREEFLRFELDRYLG
                     WPGQAPSYKIGERLWMQLREEVRAREGDGFDIKAFHRQALDIGSVGLDVLRAAFAG"
     gene            1204..3063
                     /locus_tag="VHM65_04415"
     CDS             1204..3063
                     /locus_tag="VHM65_04415"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX2704979.1"
                     /translation="MTAALAIVAIAAGVLAGAALGRRTGVPTPLVLVVAGMAASFVPG
                     FPAVVVEPDIILLGLLPPLLYAAAIRTSLIDLGRNRRNIGLLAVGLVLFTAIGVGLVI
                     WWLLPVPFPLALALGGVVAPPDAVAASAVARRIGLPRAIVSLLEAESLFNDATALVVV
                     RLAIIAAAGSVSVTSVSASFLVAAGGGIAIGLVAAWVISHIRRRLTDTVSETALGFLV
                     PWVAYLPAETVHASGVLAVVVCGVLLGHKAPLIQSATSRVAERINWTTIQFLLENAVF
                     LLIGLQARPVVSAVGGSPLGWPRIAGAAVAVLAAVILLRFLWIVPLHLLIDRIVVPGL
                     RHRVVGAVLVSWAGMRGVVTLAAAFLLPVSTHRDVLILIALAVTAGTLLLQGLTLPML
                     ARRLDAYGPDPREDALQAATVLQRAVSAGRAELDSIADETTPDAVVQTLRAQGERRTN
                     VIWERLGADREVETPSETYRRLRLAMLDAERRSVLELRARGAVEHDVLEEVLRTLDLE
                     ETMLLVVSGRTDRLAERVVRTPERVSSGCEHLDAAPTRVTPLTPDGCPECRAEGTTTV
                     HLRLCLTCGHVGCCDSSVGRHATRHNAQTGHPVIRSFEPGEAWRWCFPDEILG"
     gene            3179..3841
                     /locus_tag="VHM65_04420"
     CDS             3179..3841
                     /locus_tag="VHM65_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEX2704980.1"
                     /translation="MSPAPLELVLASASPARRGALARAGVRATVVVSHVDEEAAVREA
                     RQRHGPLDPPGQALLLARAKAADVAAGQANTLVLGCDSVLDLDGEVHGKPASAEQAVA
                     RWRRMRGRSGTLHTGHWLVDTRTPGEGGSSGALGATASTVVHFARLSDDEIEAYVATG
                     EPLLVAGAFTIDGLGGAFVTGIEGDPHNVVGLSLPVLRDLLAQIGVGWFDLARGGGAQ
                     GK"
     gene            complement(3829..>4030)
                     /locus_tag="VHM65_04425"
     CDS             complement(3829..>4030)
                     /locus_tag="VHM65_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704981.1"
                     /translation="RYAMLAFPMALLLAWALRLPERLFASSPQVKWWGLASMAALGLG
                     QQAWYTYNYLLVTHLDGTIYFP"
BASE COUNT          495 a         1531 c         1392 g          612 t
ORIGIN      
        1 gtccagctcg tggccgccga cgggtacttc gcggccctgg gcagccgcgc cgcagccggc
       61 gccgccgacc ttcgcgacgt cacccgcgcc gccctcaccg acgcggcagc gcaggccgcc
      121 ggcgcgtatg ccgagatggc cacgttcctg cgcgacgagc tgcagggcgc ctcaccggag
      181 gccgatgcgt gcggccgcga gcgctacacc cttgtctcgc agtacttcct cggcgcggtc
      241 gtcgacctcg aggagaccta ccagtggggt caggaggagc tcgcccggat cacggccgag
      301 atggcgcagg tcgccgacca gatcaagccc ggcgcgtccg tcgtcgaggc gatcgaggtc
      361 ctcaacgccg atccggccta cctgctccag ggcaccgacg cactcaagga gtggatgcag
      421 gccaaggccg acgaggccat cgccaacctg tccggcacgc acttcgacat ccccgagccc
      481 gttcgccgga tcgagtgcat gatcgccccg acccagaccg gcggcatcta ctacaccggg
      541 ccgagcgagg acttctcccg cccgggtcgc atgtggtggt cggtccccag gggggtcacc
      601 gacttccgca cctggaacga gctcaccacc gtctaccacg agggcgtccc cggccaccac
      661 ctgcaggtgg cccagaccac cttccgttcg gacctgctca acaagtggcg ccggctcgac
      721 tcgtggacca gcgggtatgg cgagggctgg gccttgtatg ccgagcgcct catggctgac
      781 ctgggctacc tcgacgaccc cggcgctcgc atgggcatgc tcgacggcca gtcgatgcgg
      841 gccgcgcggg tcgtcctcga catcgggatc cactgcggct tcgacgcccc ggccgaggtg
      901 ggcggcggcg cgtggaccta cgacaaggcc tgggccttcc tcactggcca tgcgttcggc
      961 cgggaggagt tcctccgttt cgagctcgac cgctacctcg gctggccggg ccaggcgccg
     1021 tcatacaaga tcggcgaacg gctgtggatg cagctgcgcg aggaggtgcg ggcccgcgag
     1081 ggcgacggct tcgacatcaa ggcgttccac cgccaagcac tcgacatcgg cagcgtcggt
     1141 ctcgacgtcc ttcgcgccgc cttcgccggg tgacgcggcg ggcgcggcgg taggcccggt
     1201 ccggtgactg ctgccctggc gatcgtcgcg attgccgccg gggtgctcgc tggtgccgcc
     1261 ctcggccggc gcacgggcgt gcccaccccc ctcgtgctcg tcgtggcggg catggcggcc
     1321 tccttcgtgc ccggcttccc ggccgtcgtc gtcgagcccg acatcatcct gctcggcttg
     1381 ctcccgcccc tgctctacgc ggcggcgatc cggacctcgc tcatcgacct cgggcgcaac
     1441 cggcgcaaca tcggcctgct tgccgtgggg ctcgtcctgt tcaccgcgat cggcgtcggc
     1501 ctcgtgatct ggtggctgtt gcccgtgccc ttcccgctcg ccctggcact cggcggggtc
     1561 gtcgcgccgc ccgacgcggt ggccgccagc gcggtcgccc ggcgcatcgg cctgcccaga
     1621 gcgatcgtgt cgctgctcga ggcggagtcg ctcttcaacg acgccacggc cctggtggtg
     1681 gtccggctgg cgatcatcgc cgccgcgggc agcgtgtcgg tgacctcggt ctcggcctcc
     1741 ttcctcgtcg ccgccggtgg cgggatcgcc atcggcctgg tcgccgcatg ggtcatctcc
     1801 cacatccgtc gccgactcac cgacaccgtg tccgagacgg ccctgggctt cctcgtcccc
     1861 tgggtcgcct acctgcccgc cgagacggtt cacgcctccg gcgtgctggc cgtcgtcgtc
     1921 tgcggggtgc tgctcgggca caaggcgccg ctgatccaga gcgcgacgag ccgggtcgcc
     1981 gagcggatca actggacgac gatccagttc ctcctcgaga acgccgtctt cctcctcatc
     2041 ggcctgcaag cccgcccggt cgtgtcggcc gtgggtggct ctcccctcgg ctggccgagg
     2101 atcgccggtg ccgcggtggc cgtactcgcg gcggtgatcc tcctgaggtt cctctggatc
     2161 gtcccgctgc acctgctcat cgaccgcatc gtcgtgccgg gcctgcgtca ccgggtcgtc
     2221 ggcgccgtgc tcgtgtcctg ggccggcatg cgcggtgtcg tcaccctcgc cgccgccttc
     2281 ctcctgcccg tgagcaccca ccgggacgtg ctcatcctca tcgccctcgc cgtcacggcc
     2341 ggcaccttgc tcctgcaggg cctgaccctg cccatgctgg cccggcgcct cgacgcttat
     2401 ggtcccgacc cgcgcgagga cgcgcttcag gctgccacgg tcctgcagcg ggcggtctcc
     2461 gcagggcggg ccgagctcga ctcgatcgcc gacgagacca caccggacgc ggtcgtgcag
     2521 accctgcgcg cccagggcga gcgtcggacc aacgtcatct gggagcgcct cggcgcggac
     2581 cgggaggtcg agaccccgag cgagacctac cgtcggctgc gcctggccat gctcgacgcc
     2641 gagcgccgca gcgtcctcga gctgcgagcc cggggcgccg tcgagcacga cgtcctcgag
     2701 gaggtcctgc gcacgctcga cctcgaggag accatgctcc tcgtcgtctc ggggcgcacc
     2761 gaccgcctgg ccgagcgggt cgtccgcacg cccgaacgcg tctcgtcggg ctgcgagcac
     2821 cttgacgccg caccgacgag agtcacccct ctcacgcccg acggatgtcc cgagtgccga
     2881 gccgagggca cgaccacggt ccatctgcgg ctgtgcctca cctgcgggca cgtcggctgc
     2941 tgtgactcct cggtgggccg gcacgcgacc cgccacaacg cgcagaccgg gcaccccgtc
     3001 atccgctcgt tcgagccggg ggaagcctgg cgctggtgct tccccgacga gatcctgggc
     3061 tgatcgccag gtctgggtcg ccggctgttt tgggggacgt cgcgacgagc gggccatgga
     3121 cgcttcggcc cggcaggtgg gctctcggcg ggctaggcgt cggctaggct cgatcctcgt
     3181 gtcacccgca cccctcgagc tcgtgctcgc ctcggcctcc cccgcccggc ggggcgccct
     3241 ggccagggcc ggggtcaggg cgaccgtcgt ggtcagccac gtcgacgagg aggccgccgt
     3301 ccgcgaggcg cggcagcgcc atggaccgct cgacccgccc ggccaggccc tgttgctggc
     3361 ccgtgccaag gctgcggacg tcgcggccgg ccaggccaac acccttgtcc tgggctgcga
     3421 ctcggtcctc gacctcgacg gcgaggtcca cggcaagccc gcctcggccg agcaggccgt
     3481 cgcgcgctgg cggcgcatgc gcggacgctc gggcaccctg cacacgggcc actggctggt
     3541 cgacacccgc accccgggcg agggcgggtc cagcggggca ctcggtgcca ccgcatcgac
     3601 cgtcgtgcac ttcgcgcggc tctccgacga cgagatcgag gcgtatgtcg ccaccggtga
     3661 acccctgctg gtcgccgggg cgttcacgat cgacggcctc gggggcgcgt tcgtcaccgg
     3721 catcgaggga gacccgcaca acgtcgtcgg cctgagcctg ccggtcctgc gggacctgct
     3781 cgcccagatc ggcgtgggct ggttcgacct cgcccgcggg ggcggcgctc aggggaagta
     3841 gatcgtcccg tcgaggtggg tcacgagcag gtagttgtag gtgtaccacg cctgttggcc
     3901 cagcccgagc gccgccatac tcgccagccc ccaccatttg acctggggcg agctggcgaa
     3961 caggcgctcg ggcaggcgca gcgcccaggc caggagcaac gccatcggga aggcgagcat
     4021 cgcatagcgc
//
LOCUS       DATAIX010000298         5194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_275285, whole genome shotgun sequence.
ACCESSION   DATAIX010000298 DATAIX010000000
VERSION     DATAIX010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275285"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VHM65_04430"
     CDS             <1..573
                     /locus_tag="VHM65_04430"
                     /inference="COORDINATES: protein motif:HMM:NF042550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6636 domain-containing protein"
                     /protein_id="HEX2704982.1"
                     /translation="TSVVTLTRTPTASSSAPTSPTAAPTTRTLTPVEVTELAADKVAP
                     DYPFQTPSKRIACVLMGTSAGGGPPYAVRCDFTGKAQWTVPKPSGGCALDWGGDSANL
                     RSVGLSGDPARPHVNCISDSVKDPAATVLAYGTGIRIGSITCESRSTGLRCLDPSGGS
                     FEISLQALYMQCTQPGGTLRDVPAAAEPCA"
     gene            644..2545
                     /gene="typA"
                     /locus_tag="VHM65_04435"
     CDS             644..2545
                     /gene="typA"
                     /locus_tag="VHM65_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX2704983.1"
                     /translation="MSMKTRGDVRNVAIIAHVDHGKTTLVDKMLWQTGAFGEHEHVDE
                     RAMDSGDLEREKGITILAKNTAVRYAGPAAAAVGQPGGVTVNIIDTPGHADFGGEVER
                     GLSMVDGVVLLVDASEGPLPQTRFVLRKALAKKLPVVLCINKVDRPDSRIAEVVDEVY
                     ELFLDLDADEHQIDFPIIYASARAGRASLARPEDGGLPDSPDLEPLFRTILETVAAPS
                     YDDEAPLQAHVTNLDASNFLGRLALLRVHNGTIRKGQHVAWIRNADHSIERVKITELL
                     ITEALDRVPADSAGPGDIIAVAGIPGITIGDTLADLDDPRPLARISVDEPAISMTIGT
                     NTAPMAGRVKGAKVTARLVKDRLDRELVGNVSIRVLPTERPDTWEVQGRGELALAILV
                     EQMRREGYELTVGKPQVVTREVGGKVHEPVERLTIDAPEEFMGTITQLLAMRKGRIEQ
                     MTNHGTGWIRMEFLVPSRGLIGFRTEFLTDTRGTGIAHHVFEDYEPWFGEIKTRLTGS
                     LVADRTGAATSYAMFNLQARGTMFVEPTTEVYEGMIVGENSRADDMDVNICKEKKLTN
                     VRSSTSEVLERLIPPRALSLEQSLEFCREDECVEVTPQAVRIRKVVLDQVERGRTAAR
                     VRAAAKADR"
     gene            2547..4385
                     /locus_tag="VHM65_04440"
     CDS             2547..4385
                     /locus_tag="VHM65_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2704984.1"
                     /translation="MSTGSRRWRRPGPVPGRRWPRGRSLAPALAPALALLMVAGACTV
                     VPRQTLPTDPAVRGRGGTLQVLSTGQMRSLDPQRLAPGPESSFATSTYLRTLTTRRPA
                     PAGALGELVPDLAVGLGGSSDGGRSWVFPLRQDVPWQDGSQVTCEDFAYGISRAFGPR
                     ADSPGSRLVRALLDIPLQTTGAQAGQPVYGGPGGSAGQDELARAVSCDGPNLRIVLRR
                     PVADLNELVTIPAFAPFKEQYDRGAEGDFIVFTCGAYMLEGNWHDDGGRFVRSRNFRL
                     LTDPVRRVKPNAVELVQNLSPEEVTARLVAATSSSASPDTVDYGRRAAVMLEGLPAAA
                     VHGFDPVGGDAALAAVRAATPSGGVDTLLVTATSGPLSTEPVRRALALATNREGYVEG
                     SMGTLLPGYSLSALPQVLAPSTDSVGSGPAGNPAQARKVLAAARATLPVPVDLAYQAG
                     DGADSRVMALVATWREAGFEVTPRALSLDGLRAAARSSTSGARPEVILTTTTPLWPSA
                     EAAIAGVLDPAWAGERIAIPVELSVPTASPPVFAAALAEPDPGLRQSQWQAIESWALR
                     SGLVIPVATQVGIRVHDPVIRGYLMDAFGVPSLAEIQLLPDETAAP"
     gene            4398..>5194
                     /gene="mshB"
                     /locus_tag="VHM65_04445"
     CDS             4398..>5194
                     /gene="mshB"
                     /locus_tag="VHM65_04445"
                     /EC_number="3.5.1.103"
                     /inference="COORDINATES: protein motif:HMM:TIGR03445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-
                     D-glucopyranoside deacetylase"
                     /protein_id="HEX2704985.1"
                     /translation="MTPVSPAPADRPARLLFVHAHPDDETLTCGTAMAHYARAGHEVV
                     VLTCTLGEEGEVIPPELAHLASDRCDTLGPWRREELREAMTRLGVRHAVLGEDRACGV
                     LSRYRDSGMAGSPAAARPGAWVNADPDEAADLIGAHIRGVRPDVVVTYDEHGGYRHPD
                     HVQTHRMTVRAVASLREHERPARLALVLTPQSWAREDRAWLRAHVPPGAGLTLLGDDA
                     PYPALVVPDEVVTHAVADPAAVPAQARALAAHRTQVTVFDGYYALSN"
BASE COUNT          735 a         1965 c         1783 g          711 t
ORIGIN      
        1 acctcggtcg tcacgctgac ccggaccccc accgcgtcgt cgtcggcgcc gacctcgccg
       61 acggcggctc ccacgacgcg gaccctgacc ccggtcgagg tcaccgaact cgcggcggac
      121 aaggtggcgc cggactaccc cttccagacc ccgagcaagc ggatcgcctg tgtgctcatg
      181 gggacctccg ccggtggcgg tccaccgtat gccgtgcggt gcgacttcac gggtaaggcc
      241 cagtggacgg tgccgaagcc ttccgggggg tgcgcgctgg actggggtgg cgacagcgcg
      301 aacctgcgct cggtgggact gtcgggcgac ccggcccgcc cgcacgtcaa ctgcatctcg
      361 gactcggtca aggacccggc agccaccgtg ctcgcctacg gaaccggcat ccggatcggc
      421 agcatcacct gcgagagccg cagcacgggc ctgcgctgcc tcgacccgtc ggggggcagc
      481 ttcgagatct cgctgcaggc gctgtacatg cagtgcaccc agcctggtgg cacgctcagg
      541 gacgtcccgg ccgcggcgga gccctgcgcc tgagccggcg cccgggtcct cgtcagccgg
      601 gggctggtcg ggcagcgcgg cgaaccctga gagaatcctc cctatgtcca tgaagacccg
      661 cggcgatgtc cgcaacgtcg cgatcatcgc ccatgtcgac cacggcaaga ccactctcgt
      721 ggacaagatg ctgtggcaga ccggggcctt cggcgagcac gaacacgtcg acgagcgggc
      781 catggactcc ggggacctcg agcgcgagaa gggcatcacg atcctggcca agaacaccgc
      841 agtccggtat gccgggccgg cggctgccgc tgtcggccag cccggcgggg tcacggtcaa
      901 catcatcgac accccgggcc acgccgactt cggtggcgag gtcgagcgcg gcttgtccat
      961 ggtcgacggg gtcgtcctgc tcgtcgacgc ctccgagggc ccgcttccgc agacgcggtt
     1021 cgtgctgcgc aaggccctgg ccaagaagct cccggtggtg ttgtgcatca acaaggtcga
     1081 ccgccccgac agccgcatcg ccgaggtcgt cgacgaggtc tacgagctct tcctcgacct
     1141 cgacgccgac gagcaccaga tcgacttccc gatcatctat gcctccgcca gggccggccg
     1201 ggcctcgctg gcccgccccg aggacggagg cctgccggac agccctgacc tggagccgct
     1261 cttccggacc atcctcgaga ccgtcgcggc gccgtcatac gacgacgagg cacccctgca
     1321 ggcccacgtg accaacctcg acgcctcgaa cttcctcggt cgcctcgccc tgctcagggt
     1381 ccacaacggg acgatccgca agggccagca cgtcgcgtgg atccgcaacg ccgaccactc
     1441 gatcgagcgg gtcaagatca ccgagctgct catcaccgag gccctcgacc gggtgcctgc
     1501 cgacagtgcc ggccccggcg acatcatcgc ggtcgccggc atcccgggca tcacgatcgg
     1561 cgacaccctg gccgacctcg acgacccccg ccccctggcc aggatctccg tcgacgagcc
     1621 ggccatctcg atgacgatcg gcaccaacac ggccccgatg gccggccggg tcaagggcgc
     1681 caaggtcacc gcacgcctcg tcaaggaccg cctcgaccgc gagctcgtcg gcaacgtctc
     1741 gatccgggtg ctgccgaccg agcgccccga cacctgggag gtccagggcc gcggcgagct
     1801 cgcgctggcg atcctcgtcg aacagatgcg ccgcgagggg tacgagctca ccgtcggcaa
     1861 gccccaggtc gtgacccggg aggtgggtgg caaggtccac gagccggtcg agcggctcac
     1921 catcgacgcg cccgaggagt tcatgggcac gatcacccag ctcctcgcga tgcgcaaggg
     1981 ccgcatcgaa cagatgacca accacggcac cggctggatc cgcatggagt tcctcgtgcc
     2041 ctcgcgcggg ctcatcggct tccgcaccga gttcctcacc gacacccgcg gcacggggat
     2101 cgcccaccac gtgttcgagg actacgagcc gtggttcggc gagatcaaga cccgtctgac
     2161 cgggtcgctc gtggccgacc gcaccggtgc cgccacgtca tacgcgatgt tcaacctcca
     2221 ggcgcggggg acgatgttcg tcgagccgac cacggaggtc tacgagggca tgatcgtcgg
     2281 ggagaactcg cgcgccgacg acatggacgt caacatctgc aaggagaaga agctcaccaa
     2341 tgtgcgctcg tcgacctccg aggtgctcga gcggctcatc cccccgcgcg cgctgagcct
     2401 cgagcagtcc ctcgagttct gccgcgagga cgagtgcgtc gaggtcactc cgcaggccgt
     2461 gcgcatccgc aaggtcgtcc tcgaccaggt cgagcgaggc cggaccgccg cgcgggtgcg
     2521 ggcggccgcc aaggcggacc gctgaggtga gcacggggag ccgacggtgg cggcgtccgg
     2581 gcccggtgcc cggccgcagg tggccgcgcg ggcgctcgct cgctcccgcg ctcgctcccg
     2641 cgctcgccct gctcatggtc gccggtgcct gcacggtcgt cccgcgccag accctgccga
     2701 ccgacccggc cgtgcgcggg cgcggcggca cgctccaggt gctcagcacc gggcagatgc
     2761 gttcgctcga cccccagcgg ctcgccccag gaccggagtc gtccttcgcc acgagcacct
     2821 acctgcgcac cctgaccacc cgcagaccag cccccgcagg cgcgctcggc gagcttgtcc
     2881 cggacctcgc ggtgggcctg ggtggctcct cggacggcgg gcgctcgtgg gtcttccccc
     2941 tgcgccagga cgtgccctgg caggacggca gccaggtgac ctgcgaggac ttcgcctacg
     3001 gcatctcgcg cgccttcgga ccgcgggcgg actcgccggg gtcgcgcctc gtccgcgccc
     3061 tcctcgacat cccgctgcag acgaccgggg cccaggcagg acagccggtg tatggcggac
     3121 ccgggggctc cgcgggccag gacgagctcg cccgggccgt gtcctgtgat ggccccaacc
     3181 tgcggatcgt cctgcgccgg ccggtcgcgg acctcaacga gctcgtcacg atccccgcgt
     3241 tcgcgccctt caaggagcag tacgaccggg gcgccgaggg cgacttcatc gtgttcacct
     3301 gtggcgccta catgctcgag ggcaactggc acgacgatgg cggcaggttc gtccgcagcc
     3361 gcaacttccg gctcctcacc gacccggtcc gccgggtcaa gcccaacgcc gtggagctgg
     3421 tccagaacct ctcgcccgag gaggtgaccg cccggctcgt cgcggcgaca tcctcctccg
     3481 cgtcgccgga taccgtcgac tacggccggc gggccgcggt gatgctcgag ggtctcccgg
     3541 ccgcggcggt ccatggcttc gacccggtcg gcggcgacgc ggccctggcc gccgtgcggg
     3601 cggcgacacc gtccggtggc gtcgacaccc tgctggtgac ggccacgtcc ggcccgctgt
     3661 cgaccgagcc ggtgcgccgg gctcttgctc tcgcgacaaa ccgggaaggg tatgtcgagg
     3721 gctcgatggg cactctgctt cctggctact ccctctcggc gctgccacag gtccttgctc
     3781 cgtcaaccga ctcggtcggc agtgggcccg caggcaaccc ggcccaggcc aggaaggtgc
     3841 tcgccgcggc cagggccacc ctgccggtcc ccgtcgacct ggcctaccag gccggcgacg
     3901 gggccgactc gcgggtcatg gcactcgtgg cgacgtggcg cgaggccggg ttcgaggtca
     3961 cgccgcgggc gctcagcctg gacgggctgc gagcggccgc gcggtcgagt acgtcgggcg
     4021 cccggcccga ggtcatcctc acgacgacaa ccccgctctg gccgtcggcc gaggcggcca
     4081 tcgccggcgt cctggacccg gcctgggcgg gtgagcggat cgccataccg gtcgagctct
     4141 ccgtgcccac cgcctccccg ccggtcttcg cggcggcgct cgccgagccc gaccctgggc
     4201 tgcggcagag ccagtggcag gcgatcgagt cctgggcgct gcgctcggga ctcgtgattc
     4261 ccgtcgccac gcaggttggg atccgggtcc acgacccggt catccggggc tacctcatgg
     4321 acgccttcgg ggtgcccagc ctcgccgaga tccagctgct ccccgacgag accgcggcgc
     4381 cgtaggagac cagggccatg acaccggtca gccccgcgcc cgcggatcgt cccgcgcggc
     4441 tgctcttcgt ccatgcccac ccggacgacg agacactgac ctgtggcacc gccatggcgc
     4501 actacgcccg ggccggccac gaggtcgtcg tcctgacctg caccctcggt gaggagggcg
     4561 aggtcatccc gcccgagctg gctcatctcg cgtccgaccg ctgcgacacg ctgggaccct
     4621 ggcggcgcga ggagctgcgc gaggccatga cccggctcgg cgtccgccat gccgtgctcg
     4681 gggaggaccg ggcctgcggc gtcctgtcgc gctaccgaga ctccggcatg gcgggatcgc
     4741 cggctgccgc ccgccccggg gcctgggtca acgccgaccc ggacgaggcc gccgatctga
     4801 tcggcgccca catcaggggc gtgcgccccg atgtcgtcgt cacctatgac gagcacggtg
     4861 gttaccggca ccccgaccat gtccagaccc atcggatgac ggtgcgggca gtggcgtcgc
     4921 ttcgtgagca tgagcgcccg gcgcgcctgg ccctggtgtt gacgccgcag tcctgggcgc
     4981 gcgaggaccg cgcgtggctg cgggcccacg tacccccagg ggccgggctg accctgctcg
     5041 gtgacgacgc gccataccct gcccttgtcg tgcctgacga ggtggtgacc cacgcggtgg
     5101 ccgaccccgc cgcggtgccg gcccaggcac gagccctggc ggcacaccgg acccaggtca
     5161 cggtgttcga cgggtactac gcgctgtcca acga
//
LOCUS       DATAIX010000299         3097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_46313, whole genome shotgun sequence.
ACCESSION   DATAIX010000299 DATAIX010000000
VERSION     DATAIX010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46313"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(378..1493)
                     /locus_tag="VHM65_04450"
     CDS             complement(378..1493)
                     /locus_tag="VHM65_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2704986.1"
                     /translation="MLTGQTLAEIARDLPSLGSDLAGPYGDALQRGVHQALEHFLSLL
                     GADVAALDPELTELYLSFGVREERHGRSLDTLLAAYRRGARSAWTHFAAAAVAADVPT
                     GQLVRLAEAIFAYIEELSSASTVGFARSTLAGAGQRDAVRQRLARAILGGEADTAVAR
                     VADLSAEAAWPMPERLGVALLPGGGLSPLPIIHPDILVVIHDSDALAVVPDGLVHGRA
                     TLERLFGSVEEVYVGTVRPAQEAPISLSHARSLHQLVVEGVLDRAPVVRAADHLPDLL
                     LHADRRLLADLTAEALAPLEAVPAGRRETLAQTLRAWLGHQGDRVLTANELGVHPQTV
                     SYRMAQLEGAFADGLRDPRSRFALLLALEGRHARRAK"
     gene            1685..>3097
                     /locus_tag="VHM65_04455"
     CDS             1685..>3097
                     /locus_tag="VHM65_04455"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX2704987.1"
                     /translation="MTITNPTSEGRPRHPTAAEPQIIQGGMGVAVSGWKLARAVSVTG
                     QLGVVSGTALELVCARRLQSGDAGGHMRRALAAFPVPEVAEWILEAYYVPGGIAPGVL
                     FRPVPRHTIESSLRLLELTVAANFVEVFLAKEGHDGLVGVNYLRKIELPLPAALYGAV
                     LAGVDAVLVGAGSPSEIPELTRRLARHEDVTLDIRVQGARSDDGLGSMGFSPRQVVPL
                     ALAPIEVPRVIGIVASADLAAGLAGTPVTRPDGFVVEGPRAGGHNAPPRGPRRVDEIG
                     QPVYDERDEVEVSKILALGLPVWLAGSYGTPQGLRDALALGAAGVQVGTAFAYSDESG
                     FDEGIKERVRREVSSGELRVRADWRVSPTGFPFQVADLPGTLSDPAVVSARKPVCDLG
                     VLRSAYVKPDGAVDYRCPAEPEAQYRRKGGREANKKGRVCLCNALLASAGLPQRRPNG
                     DVEAPLVTSGGDLRPVAALVA"
BASE COUNT          452 a         1055 c         1129 g          461 t
ORIGIN      
        1 gtgtccgtaa caagtttggt gcgggacccg cgggaccttt gattgttgct gcgtgcacga
       61 accccagatt cggttggtta cgaaggtcct cccttgagct ggtaggcggt cagatgccgc
      121 cagcctcaag ggagtcccgc ccgactcggc ggagccacgc gggtggggat tttcagtgag
      181 cagcggtcca gcgccttggc caaggcgcgc cgtggatcag ggaagatcgc gcgcaggtca
      241 ccttttcaga gtggcgccca gggcggatcc tggtgaggtc gccgcggaga gcccgttctg
      301 tccttggtgc ttcgagcccg ttgagcagcg agggtcggag ggtcgcggta aacgtagcca
      361 accgggcgca gagtctgtta ctttgcccgg cgggcgtgac gaccctcgag cgcgaggagc
      421 agggcgaagc gagaccgcgg gtcacgcaaa ccgtcggcga aggcgccttc gagctgggcc
      481 atccggtagc tcaccgtctg cggatggacc ccgagctcat tcgcggtgag cacccggtcg
      541 ccttgatgcc ccagccaggc ccgaagtgtc tgggccaggg tctcgcgccg accggccggc
      601 accgcctcga gcggcgcgag ggcctccgcg gtgaggtccg ccaggaggcg gcggtcggca
      661 tgcaagagca ggtccggcag atggtcggcg gcacggacga caggggcgcg gtcgagaacc
      721 ccttcgacga cgagctggtg cagcgaccgg gcgtgtgaca acgagatcgg cgcctcctgt
      781 gcagggcgca ccgtcccgac atacacctcc tccactgagc cgaagagcct ctcgagggtg
      841 gctcgcccgt ggaccagccc gtcaggaacg accgcgagtg cgtcggagtc gtggatcacg
      901 acgaggatgt cggggtggat gatcggcagg ggggacaggc cacccccggg cagcagggcg
      961 acgccgaggc gttcgggcat gggccaggcg gcttcggccg acaggtcggc gacgcgggcc
     1021 actgccgtgt cggcctcccc gccgaggatc gcccgggcca gccgctggcg cacggcgtca
     1081 cgctggccgg ctccggcgag ggtcgagcgg gcgaaaccga ccgtgctggc cgaggagagc
     1141 tcttcgatgt aggcgaagat cgcctcggcg aggcgcacca gctggccggt ggggacgtcc
     1201 gcggcgaccg cggccgccgc gaagtgcgtc caggcgctgc gagcaccgcg ccggtatgcc
     1261 gccagcaggg tgtcgaggct cctgccgtgg cgctcctccc ggacgccgaa ggacaggtag
     1321 agctcggtca gctcggggtc gagcgcggcg acgtcggcgc cgagcaagga caggaagtgc
     1381 tccagcgcct ggtgcacccc acgctgcagc gcgtcgccat agggaccagc gaggtcgctg
     1441 cccagggagg gcagatcgcg cgcgatctcg gccagggtct gcccagtgag caccggtaga
     1501 gtcgggcgca acacggctga aatggcgggg tcgatgtcgg cccacggtcg gtctccagac
     1561 gatcttgtca ccacgagcta aatccttcgg ccgaatctga tgctgacggg gctaacaccg
     1621 ttgtggttct cgctccgaca ctagaggtac tgagatataa tcgtgccgtg aaagtgaaat
     1681 cgtcatgacg atcaccaacc ccacctccga gggtcggccg cgccatccca ccgccgctga
     1741 gccccagatc atccagggtg gcatgggagt tgccgtctcc ggctggaagc tggcccgcgc
     1801 cgtgtccgtc acgggtcagc tcggggtcgt ctccggcacc gcgctcgagc tcgtctgcgc
     1861 ccgacggctg cagagcgggg acgccggcgg ccacatgcgc cgagccctcg cggcattccc
     1921 cgtccccgag gtcgccgagt ggatcctcga ggcctactac gtcccgggag gcatcgcccc
     1981 cggcgtgctc ttccggcccg tgccgcgcca taccatcgag tcgtccctgc ggttgctcga
     2041 gctcaccgtc gcggcgaact tcgtcgaggt cttcctcgcc aaggaagggc acgacggcct
     2101 cgtcggcgtc aactacctgc gcaagatcga gctgcccctg cccgcggcct tgtatggcgc
     2161 cgtcctcgcc ggggtcgacg cggtgctcgt cggcgcggga agcccgtcgg agatccccga
     2221 gctgacccgc cggctggctc gccacgagga cgttaccctc gatatccggg tccagggcgc
     2281 ccggtcggac gacggcctcg gctcgatggg cttcagccct cgccaggtcg tgccgctcgc
     2341 gctggcgccg atcgaggtcc cccgcgtcat cggtatcgtc gcatcggccg acctcgcggc
     2401 cggcttggcc ggcactcccg tgacccggcc cgacgggttc gtcgtcgaag gtccgcgagc
     2461 gggcgggcac aacgcaccgc cgcgcggtcc gcgtcgcgtc gacgagatcg gccagcccgt
     2521 gtatgacgag cgggacgagg tcgaggtgtc caagatcctc gcgctgggac tcccggtgtg
     2581 gctcgccggc tcctacggca ccccgcaggg gctgcgcgac gcgctggccc tcggtgccgc
     2641 cggagtgcag gtggggacgg cgttcgccta ctcggacgag tcgggtttcg acgaggggat
     2701 caaggagcgc gttcgccgcg aggtcagctc tggcgaactg cgcgtgcgcg ccgactggcg
     2761 cgtctcgccg accgggttcc ccttccaggt cgccgatctg cctgggaccc tgtcggaccc
     2821 ggccgtcgtc agcgcacgca aaccggtgtg cgacctcggg gtcctgcgct cggcctacgt
     2881 gaagccggac ggggccgttg actaccggtg cccggccgaa cccgaggcac agtaccggcg
     2941 caaggggggg cgtgaggcca acaagaaagg ccgcgtctgc ctgtgcaatg cgctcttggc
     3001 cagtgctggt ctgccgcagc ggcggccgaa cggcgacgtc gaggcgcccc ttgtcacatc
     3061 tggcggtgac ctccgaccgg tcgcggcgct cgtggcc
//
LOCUS       DATAIX010000300         3304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_16062, whole genome shotgun sequence.
ACCESSION   DATAIX010000300 DATAIX010000000
VERSION     DATAIX010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16062"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1881)
                     /locus_tag="VHM65_04460"
     CDS             complement(163..1881)
                     /locus_tag="VHM65_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX2704988.1"
                     /translation="MTQAIPPPVTVLVVGATHGALLGPHDDLAAPAAQVLDRAAHLAT
                     AESPRWVVWSASRDLGALAQRGVPLGRAWDLAEVHRLIHGGWRADPTTVWAGAAGLDL
                     DAIPAQARGDLFDAVDDTDPEHLLRSDGYLHPDRDGPAWVSSPGRARASASAARAVFD
                     RQWTALSALGGTAGPRAIHTAYAESAAALLCLELERDGLPIDRAAAEELIGRAAGPRP
                     RSDTDAARIRGERDRRVLDLLPGGGTVDLRNPAQVKELLTSVGVEVPHTRAWVLEPYR
                     QSSPVVAALLDWRKDERIATTYGYRWLDEHVGPDDRLRGAWTACDGGAGRMTAQSGLH
                     NLPAPLRHAVRAEPGWVFVRADLGQIEPRVLAAVSGDQAFAEATRADDLYAPVAAKLG
                     VDRAVAKVAVLAAMYGQRSGAAGEALADLVRAYPVAMRFLDDAYDAGVARRPLRTYGG
                     RFLPLGAGPATLGPEDSGRGRYARNAVVQGAAAELFKAWVATIRHTLLDGETALGRDG
                     EPRRAQVSLCLHDEVLIHSPHEQAPGIERLLHTALDESARRWTGTQAVRFVADTTIVR
                     RWSEAK"
     gene            2007..2750
                     /locus_tag="VHM65_04465"
     CDS             2007..2750
                     /locus_tag="VHM65_04465"
                     /inference="COORDINATES: protein motif:HMM:NF024197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HEX2704989.1"
                     /translation="MRSLFAMPAPEGTPRREGTVAEAPRSALDQADRQSALEEPSVLG
                     GAATRLVERLLSIGIDGAGPVRPAREAAQAALRKHGTAERGISAIITSHVLVGGAGGF
                     LTGLGGFVTVPVAVPVNVTEFYLVATRMVASIASARGYDVDEPNIRLAVLLTLVGADS
                     DDLLRKAGVAAAGPVSRMVAQRLPGPALMMLNKGVGFRLIAQVGRSGPARFGRAIPLI
                     GGGVSAGLDVYLLAHIARHAKAEFPPRAG"
     gene            2902..>3304
                     /locus_tag="VHM65_04470"
     CDS             2902..>3304
                     /locus_tag="VHM65_04470"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEX2704990.1"
                     /translation="MSARRVRWGVLRTLATAVRTAATPGGPGLGERLAALPRLVRAAA
                     RRECRGTSGRTLGFMGAAVLYVLSPLDLVPEGLFALAGFADDTLVVAWLAAALITATE
                     DFLALERGAPGTPSPAASPPPPGTGTYGADGR"
BASE COUNT          412 a         1218 c         1238 g          436 t
ORIGIN      
        1 gcggcagtac gacgcgttcg cgcggggccg cgaacgccag agcctgctcg ccccggacca
       61 gggctccgtg ccgtcggcag aggagatcgg cgccgagctc gaggcgttcc tcaaggacgt
      121 caccgacccc aacgcgggtc aggcgtaggg actcccggcg ggctacttcg cctcggacca
      181 ccgccgcacg atcgtcgtgt cggcgacgaa acggacagcc tgcgtgcccg tccagcgccg
      241 ggcgctctcg tccagtgccg tatgcagaag tcgctcgatc cccggcgcct gctcgtgcgg
      301 gctgtggatg aggacctcgt cgtgcaggca cagcgagacc tgggcccgcc tgggttcgcc
      361 gtcgcgcccg agcgccgtct caccgtccag gagggtatgg cggatcgtcg ccacccacgc
      421 cttgaacagc tcggcggcgg cgccctggac cacggcgttg cgtgcatagc gcccccgccc
      481 gctgtcctcg gggcccagcg tggccgggcc tgctcccagg ggaaggaacc gcccgccata
      541 cgtgcgcagg ggccggcgcg cgactccggc gtcgtacgcg tcgtcgagga agcgcatcgc
      601 caccgggtag gcgcgcacga ggtccgcgag cgcctcgcct gcggcaccgg agcgctggcc
      661 gtacatcgcg gcgagcaccg cgaccttggc cacggcccga tcgaccccga gcttggccgc
      721 cacgggggcg tagaggtcgt cagcgcgggt cgcctcggca aaggcctggt cacccgacac
      781 ggccgcgagc acgcgcggct cgatctggcc gaggtcggcc cggacgaaga cccagccggg
      841 ctcggcccgc accgcgtgac gcaggggagc gggcaggttg tgcagcccgc tctgggccgt
      901 catgcgcccc gcgccgccgt cgcacgccgt ccaggcgccg cggagccggt cgtcggggcc
      961 gacgtgctcg tcgagccacc ggtagccgta ggtcgtcgcg atccgctcgt ccttgcgcca
     1021 gtcgagcagc gcggcgacga caggcgagct ctgccggtac ggctcgagca cccaggcgcg
     1081 ggtgtgcggg acctcgaccc cgacgctggt gagcagctcc ttgacctggg cggggttgcg
     1141 caggtcgacg gtgccgccgc ccggcagcag gtcgaggacc cgccggtccc gctcgcccct
     1201 gatccgtgcg gcgtcggtgt cggagcgcgg gcggggaccg gcggcccggc cgatgagctc
     1261 ctcggcggcc gcgcggtcga tcgggaggcc gtcgcgttcg agctcgaggc acagcagggc
     1321 cgcggcggac tcggcatacg ccgtgtgtat ggcgcgcggc cctgcggtgc cgcccagggc
     1381 cgacagcgcg gtccactgac ggtcgaagac ggcccgggcc gcgctggcac tggcccgggc
     1441 ccgtcccggt gagctgaccc aggccgggcc gtcccggtcg ggatgcagat agccgtcgga
     1501 gcgcagcagg tgctcagggt cggtgtcgtc gacggcgtcg aagaggtcac cgcgggcctg
     1561 cgcgggtatg gcgtcgaggt cgagccctgc cgcgccggcc cacaccgtgg tgggatcggc
     1621 ccgccacccc ccgtggatga gccggtggac ctcggccagg tcccaggcac ggccgagggg
     1681 tacaccacgc tgggccaggg ctccgaggtc gcgcgacgcc gaccagacca cccatcgggg
     1741 cgactccgcc gtcgcgaggt gggcggcccg gtcgagcacc tgcgcggctg gagcggcgag
     1801 atcgtcgtgc gggccgagga gggcaccgtg tgttgcgccc acgacgagga cggtgaccgg
     1861 tggcgggatg gcctgggtca ccgcggcgcg cctcgtcgtg atgccttcgc ctccatgcgc
     1921 tcatccaagc gcaccgcacc gacgtgcgag gatgagcctt ccgggcggac ctgcaagcag
     1981 tccgcggcta agggaaggat cacgacgtgc gtagtttgtt cgccatgcca gccccggagg
     2041 gcacgcctcg ccgcgagggc accgtcgccg aggcacctcg ctcggcgctg gaccaagcag
     2101 accggcagag cgcgctcgag gagccgtcgg tgctcggcgg ggccgcgacc cggctcgtcg
     2161 aacggctgct gtccatcggc atcgacggcg ccggaccggt gagacccgcc cgtgaggcgg
     2221 ctcaggcggc cttgcgcaag cacgggaccg ccgagcgcgg catctcggcc atcatcacct
     2281 cgcatgtcct cgtcggcggc gcgggtggct tcctcaccgg cctgggaggt tttgtcaccg
     2341 tcccggtggc cgtgccggtc aacgtcaccg agttctacct cgtcgcaacc cggatggtgg
     2401 cctcgatcgc gtcggctcgt ggctacgacg tcgacgagcc caacatccgg ctggccgtcc
     2461 tgctcacctt ggtcggggcc gacagcgacg acctgctgcg caaggcgggc gtcgccgcag
     2521 ccgggccagt gtcccggatg gttgcccagc gactgcctgg cccggcactg atgatgctca
     2581 acaagggggt cggcttccgg ctcatcgccc aggtcggcag gagcgggccg gcccgcttcg
     2641 gacgggccat ccccctcatc ggcggcggcg tgagcgccgg tctcgacgtc tacctgctcg
     2701 cccacatcgc gcgccacgcc aaggccgagt tcccgccccg tgcgggctga gccggctccg
     2761 cctggtcacg ggctggtcac gaagtgacga cgaggtcgac gggtgtggaa tcggcggcgg
     2821 gcgccactcg ttagccctgg tgcgcccacc cagggcgccc ccgcggaccg ggtctgcggg
     2881 cggggacgtg ggaggagacc gatgagtgca cgccgagtcc gctggggcgt cctacggacg
     2941 ctggcgaccg ctgtccggac cgccgccacc cctgggggcc ccgggctcgg cgagcgcctc
     3001 gccgcgctgc cgcgccttgt gcgggcggcc gcgcgccgag agtgccgcgg gaccagtggc
     3061 cgcacgctgg ggttcatggg agccgccgtc ctgtacgtcc tgtccccgct cgacctcgtc
     3121 cctgaggggc tcttcgcgct ggccggcttc gccgatgaca ccctcgtggt cgcctggctg
     3181 gccgcggcgc tcatcaccgc gaccgaggac ttcctcgcgt tggagcgcgg cgcgcccggc
     3241 acgccgagtc ctgccgcgag cccacccccg cccggcactg gcacctatgg cgcagatggc
     3301 aggt
//
LOCUS       DATAIX010000301         2987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_185915, whole genome shotgun sequence.
ACCESSION   DATAIX010000301 DATAIX010000000
VERSION     DATAIX010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185915"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..976
                     /locus_tag="VHM65_04475"
     CDS             143..976
                     /locus_tag="VHM65_04475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX2704991.1"
                     /translation="MRAIWKGAVSFGLVNVPVRLYSATENHDVQFRQVHREDGGRIRY
                     RRFCSVDDAEVSYDDIARGFETEDGQMVVLTDEDFHDLPAASSKEIAVEKFVPADQID
                     PMLLDKSYYLEPDKTSAKPYALLREALVAADRMAVVTVSIRTRMTMAVLRVRDGVIVM
                     QTMLWPDEIRSPEFASLGDDLQVTDKELAMAHMLVESLAGDYEPQDYEDDYAVALAAL
                     VKAKVEGGEVAVLPAAKDGSGEVVDLLEALQRSVDRAKAARGEPVDTHAKAKAKASKA
                     G"
     gene            1008..1883
                     /locus_tag="VHM65_04480"
     CDS             1008..1883
                     /locus_tag="VHM65_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEX2704992.1"
                     /translation="MPEGHTLHRLAASLNDAFAGAAPVVSSPQGRFTDGAGLLDGRPL
                     IEASAWGKHLFAEFAGERHLHVHLGLIGSFDVSAIGPYGAVPPPVGQVRLRLATDRHV
                     ADLRGATLCEVATPGEVDSVLARLGPDPLRADADPDRAWVRITKSPRPIADLLMDQGV
                     LAGVGNVYRSEVLFRHRVDPMRPGRETRPRTWAAIWSDLLLLMPIGVQLGQIVTMDDQ
                     VEASLAGEYDASAVPTSATGVSSWRPNPQGALERRYYVYKRQGEPCRVCGARVRTQVV
                     AGRNLFWCGRCQRRR"
     gene            1904..2176
                     /locus_tag="VHM65_04485"
     CDS             1904..2176
                     /locus_tag="VHM65_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704993.1"
                     /translation="MSRESDLATLVTQAQQAFQGGQTIFIARFKTGAFSQPGFGEVES
                     WEEMVLAVERLGWVLAHWSTSTDASSNVSAYPVFRRPTPGHPQDRQ"
     gene            2311..>2987
                     /locus_tag="VHM65_04490"
     CDS             2311..>2987
                     /locus_tag="VHM65_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX2704994.1"
                     /translation="MFERFTDRARRVVVLAQEEARMLNHNYIGTEHILLGLIHEGEGL
                     AAKALENLDISLAAVREQVQEIIGQGQQAPTGHIPFTPRAKKVLEYSLREGLQLGHTY
                     IGTEHILLGLIREGEGVAAQVLVKLGADLNKVRQQVIQLLAGYQGGQGEKAGAGVGQG
                     SQAEGTPAGSLVLDQFGRNLTQAAREAKLDPVIGREKEIERVMQVLSRRTKNNPVLIG
                     EPGVGKTA"
BASE COUNT          482 a         1039 c         1040 g          426 t
ORIGIN      
        1 gaccgccata tcagctcgct ggggtggtgt acgccgtgcc cgcgaacggc gggcacggca
       61 tagcctgccg cacagacacc tcagcgggtc ctcacagggc atccgctgga cctgcggggc
      121 gtgcaacagt catgctgtgc cgatgcgggc aatctggaag ggcgcggtgt cgttcgggct
      181 ggtcaacgtg ccggtgcggc tctactcggc gaccgagaac cacgatgtgc agttccgcca
      241 ggtgcaccgc gaggacggcg ggcgcatccg ctaccggagg ttctgctcgg tcgacgacgc
      301 cgaggtctcc tacgacgaca ttgccagagg cttcgagacc gaggacggcc agatggtcgt
      361 gctcaccgac gaggacttcc acgatctgcc cgccgcgtcg agcaaggaga tcgcggtcga
      421 gaagttcgtc ccggccgacc agatcgaccc gatgctgctc gacaagtcct actacctcga
      481 gccggacaag acctcggcca agccctatgc cctgctccgc gaggcgctgg ttgcggcgga
      541 ccggatggcg gtcgtcaccg tgtccatccg cacccgcatg acgatggccg tcctgcgcgt
      601 gcgcgatggc gtcatcgtca tgcagacgat gctgtggccc gacgagatcc gctcgcccga
      661 gttcgccagc ctcggcgacg acctccaggt gaccgacaag gagctcgcca tggcgcacat
      721 gctcgtcgag tccctcgccg gtgactacga gccgcaggac tacgaggacg actatgccgt
      781 cgcgctcgcc gcactcgtca aggccaaggt cgagggcggt gaggtcgccg tgctcccggc
      841 tgccaaggac ggctccgggg aggtcgtcga cctgctcgaa gccctccagc gcagcgtcga
      901 ccgggccaag gcggcccgcg gcgagccggt ggacacgcac gccaaagcga aggccaaggc
      961 gagcaaggcg gggtaagggc ggccggcgcg ggccgtaggg tcacctcatg cctgaaggtc
     1021 acacgctgca tcggctcgcg gcgtcgctca acgacgcctt cgccggcgca gcgcccgtcg
     1081 tgtcgagtcc gcaagggcgg ttcaccgacg gggcgggact cctggacggc cggccgctca
     1141 tcgaggcgtc ggcatggggc aagcacctgt tcgccgagtt cgcgggggag cgccacctcc
     1201 acgtccacct cgggctcatc ggcagcttcg acgtcagcgc gatcggcccg tatggcgccg
     1261 tccccccgcc cgtggggcag gtgcggctgc ggctcgcgac cgaccgccat gtcgcggacc
     1321 tgcgcggcgc gaccctgtgt gaggtggcaa cacccggtga ggtcgactcg gtcctggcgc
     1381 ggctcggccc ggacccactc cgggccgacg ccgacccgga tcgggcctgg gtacgcatca
     1441 ccaagagccc ccggcccatc gccgacctcc tcatggacca gggcgtgctc gccggtgtcg
     1501 gcaacgtcta ccgcagcgag gtgctcttcc ggcaccgggt cgacccgatg cggcccgggc
     1561 gggagactcg cccgcgcacg tgggctgcga tctggtcaga cctgttgctg ctcatgccga
     1621 ttggtgtcca gctcgggcag attgtcacca tggacgacca agtcgaggca agcctggcag
     1681 gcgagtatga cgcgtcggcc gtgcccacgt cggcgacggg tgtctcgtcg tggcgcccta
     1741 acccccaggg cgccctcgag cggcggtact acgtctacaa gcggcagggc gagccgtgtc
     1801 gtgtctgcgg cgcgcgggtc cgcacccagg tcgtcgccgg gcgcaacctc ttctggtgcg
     1861 gccgctgcca gcgccgccgg taggcgcccg gtagattcgc cccatgagcc gagaaagcga
     1921 cctggctacg ctcgtgaccc aggcccagca ggcgttccag ggagggcaga cgatcttcat
     1981 cgcccggttc aagaccggtg ccttcagcca gcccgggttc ggagaagtcg agagctggga
     2041 ggagatggtc ctggcggtgg agcggctcgg gtgggtcctg gctcactggt cgacctcgac
     2101 cgacgcgagc agcaacgtca gtgcctatcc cgtcttccga cggccgacgc ccggtcaccc
     2161 ccaggaccgg cagtaggcgg accggcaggc tcgcgacccc atacagggaa cacgccggta
     2221 tgccgcggcg ttgggtcgtg cgtgagaccc ggagcatgtt ccaggggcca cgggtagcat
     2281 cgagagcgtc tcgataggca ggagaaggcg atgttcgaac ggttcaccga ccgcgctcgg
     2341 cgtgtggtcg tgctcgcgca ggaagaggcg cggatgctca accacaacta catcggcacc
     2401 gaacacatcc tgctcggact catccacgag ggtgaggggc tggctgccaa ggcgctcgag
     2461 aacctcgaca tctcgctggc ggccgtgcgc gagcaggtcc aggagatcat cggccagggc
     2521 cagcaggctc ctaccgggca catccccttc actccgcgcg ccaagaaggt cctcgagtac
     2581 tcgctgcggg agggcctgca gctcggccac acctacatcg gcaccgagca catcctgctc
     2641 ggcctcatcc gcgagggtga aggcgttgcc gcccaggtgc tcgtcaagct cggcgccgac
     2701 ctcaacaagg tccgccagca ggtcatccag ctgctcgccg gctaccaggg tggccagggc
     2761 gagaaggccg gtgccggtgt cggccagggc agccaggccg agggcacccc ggcgggctcg
     2821 ctcgtgctcg accagttcgg ccgcaacctc acccaggccg cccgcgaggc caagctcgac
     2881 ccggtcatcg ggcgcgagaa ggagatcgag cgggtgatgc aggtcctgtc ccgccggacc
     2941 aagaacaacc cggtcctcat cggcgagccc ggcgtcggca agaccgc
//
LOCUS       DATAIX010000302         1788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_245452, whole genome shotgun sequence.
ACCESSION   DATAIX010000302 DATAIX010000000
VERSION     DATAIX010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245452"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..1562
                     /locus_tag="VHM65_04495"
     CDS             279..1562
                     /locus_tag="VHM65_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704995.1"
                     /translation="MRLLDLTPEQAHLPGPIQTGMFDSAPGLVDARSAPAAKVAFYSA
                     LFGARKDAYAVRWENTRSGASGWMPAVRGGWRRGVPAAHRAYLPLTEAVLTAHLSGQD
                     EVGLYPMLEGDRCGWVAADFDGPAAMLDALAYLKAGRAEGAPVALEVSRSGAGAHAWL
                     FFTSPVPAALARQVASGLVREAIALRGRMSLSSYDRLFPAQDTLQTGGLGNLIAAPLQ
                     GRCRRRGTTVFLDLATLEPHEDQWAYLSTLGRLSPRELDRLARRLGRVAVGVGVDRLR
                     AATSTRICVPTPAVIHVRLGSTITVQGADLPPALMATLTHAASMPNPIFYERQRRRAS
                     TWDTPRFLRSYDETIDGDLVLPRGLRDRLDELVTQAGSRLEVLDERAPGRRIGSRYAP
                     TSPRTSGPRTTRSFLMTSASWSPRPARARPSSPVP"
BASE COUNT          241 a          687 c          610 g          250 t
ORIGIN      
        1 ctcggtcggc gccccggatc cgccaggccg ctcatcattg cgtccccgcg catgcgcggc
       61 cggcctgtga cgagcggacc ggacgcagaa acgggtgagc ggggccaagg acagtcagcc
      121 cgagctaccc tgacgaacgg cggctgcgga accatggggg ggtggacacc gacgctcctt
      181 ccgacgagac gaccggcggc ccgcgctctg atcttgatcg cgagctcgcg gacctgcggg
      241 acgaggttgg tcggcttcgc acggagaacg cgcgcctgat gcggctgttg gacctgaccc
      301 ctgaacaggc gcacctgccg ggaccgatcc agacgggtat gttcgactcc gctccgggac
      361 tggtggatgc caggtcggcc ccggccgcga aggtggcgtt ctactcggcg ctgttcggcg
      421 cccgcaaaga cgcttacgcg gtgcggtggg agaacacccg ctcgggcgcc tcggggtgga
      481 tgccggccgt gcgcggaggc tggcgccgtg gcgttccggc cgcgcaccgc gcgtacctgc
      541 cgctgaccga agcagtgttg acggcgcacc tgtcgggcca ggacgaggtc ggcctgtacc
      601 ccatgctcga gggtgaccgc tgcgggtggg tcgccgccga cttcgacggc cccgccgcga
      661 tgctcgacgc gctggcgtac ctcaaggcag gccgtgccga aggcgcgccg gtggccctgg
      721 aggtgtcccg ctcgggcgcc ggcgcgcacg cctggctctt cttcacctcc ccggtcccag
      781 cggccctggc ccggcaggtc gccagcgggc tggtgcgtga ggcgatcgcg ctgcgggggc
      841 ggatgagcct atccagctac gaccggctgt tcccggccca ggacaccctc cagaccggtg
      901 ggctgggcaa cctcatcgca gccccgctgc aggggcgatg ccgccgccgc ggcaccacgg
      961 tgttcctcga cctggcgacc ctggaaccgc acgaggacca atgggcctac ctgtccaccc
     1021 ttggccggct gtccccgcgt gagctggatc ggctggcacg gcgactcggg cgggtggccg
     1081 tcggggtcgg cgtggaccgg ctgcgagcag cgacgtcgac acggatctgc gtgccgaccc
     1141 ctgccgtcat ccacgtccgg ctcggctcga cgatcacggt gcagggcgcc gacctgccgc
     1201 ctgccctgat ggccaccctc acccacgctg cctcgatgcc caacccgatc ttctacgagc
     1261 gccaacgccg ccgcgcctcg acctgggata ccccgcggtt cctgcgcagc tacgacgaga
     1321 ccatcgacgg tgacctcgtc ctgccccgcg gcctccggga ccgcctggac gagctcgtca
     1381 cccaagccgg cagccggctc gaagttctcg acgaacgagc ccccgggcgc cgcatcggtt
     1441 cgcgctacgc gccgacctcg ccccggacca gcgggccgcg cacgacgcgc tcgttcctca
     1501 tgacctcggc gtcctggtcg ccccgcccgg cgcgggcaag accgtcatcg cctgtgccct
     1561 gatcgccact cgcggcgtct ccaccctcgt cctcgtcgac cgaaagaccc tcgccgacca
     1621 atggcgtacc aggatcggcg agttcctcga cgtcacgccc ggccaacgcg gcggaggcgg
     1681 cgccagaacc agcggcgtca tcgacgtcgc caccctgcag acgctgaccc gacgagccga
     1741 cctcgccgcc ctcaccgccc tcgccgcccg atatggcctc gtcgtcgt
//
LOCUS       DATAIX010000303         3691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_16208, whole genome shotgun sequence.
ACCESSION   DATAIX010000303 DATAIX010000000
VERSION     DATAIX010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16208"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="VHM65_04500"
     CDS             <1..1095
                     /locus_tag="VHM65_04500"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX2704996.1"
                     /translation="IYLGECRHMGINVLPPDVNSSIANFAAVGADIRFGLAAVRNVGV
                     NVVEAIAVTRAQKGAFASFEDFIRKVPMVVCNKRTVESLIKAGAFDSLGDTRFGLFRV
                     HEAYVDALVDEKRQEAIGQDSLFGGFGEATGPGAGDFVVLPTTPRTEWDKQALLAFER
                     EMLGLYVSDHPLLGIEHILAQHADTSIVSLFGDEPKPDGTSVTIAGLITGLQIKRTKK
                     GDLWAVATVEDLEGSIECLFFPASYIAVSAMLGHDVVCAVKGRVSSRDDSVSIIAQDL
                     TLPDIVEGPRGPVVLTLPVARATSTVVERLRRVLREHPGGTEVHVRLVQPGRSTLMTL
                     DDAFRVAASPALYGDLKALLGPACLGERVP"
     gene            1092..2192
                     /locus_tag="VHM65_04505"
     CDS             1092..2192
                     /locus_tag="VHM65_04505"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX2704997.1"
                     /translation="MSPGPASLAHTRSSRMASLGIAPRRFTSPAAVVLGLGAVQAQDY
                     AAASWALGLRCASGTLADVVQAVTDREVVRTWPMRGTLHLVPAADAGWMCHLLTGPPI
                     RAAARLFAHLGLTEEVLGTARGAIERALTGGVALSRPELFALLAGLGIDPSAQRGIHL
                     LGRFSQQGLLCQGVPQGRQPTFTLLEEWVPGPNRPTREEALATLARRYVGGHGPVTER
                     DFAWWTGQTLGFAREAFGLIDADVERVSVDGPPHVVLRDAPTSLPRHGAHLLPGYDEL
                     ILGYQDRSATMDAATLARVVPGSNGVFQPTILANGRVVGTWTRMTTSSGVVVTARPFA
                     PLSRTRLASVGRAAASYGRFLGAMISMRLERT"
     gene            2257..>3691
                     /locus_tag="VHM65_04510"
     CDS             2257..>3691
                     /locus_tag="VHM65_04510"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2704998.1"
                     /translation="MIQPPVAKRVNHIREHHGERVVDEYEWLRDAKNPEVIAHLTAEN
                     RYAEERLAHLAPLREAIFQEIKGRTQETDLSVPVALDGWWYYARSVQGKQYALQCRAP
                     RRPGEPRPHLTPEQPVPGEQLLLDGNVEAGESDYFALGASEVSIDGDLLAFAADHVGD
                     ERFAVTVRRIATGEIIDTAVSDTGYGLVWSLDGRYLFYTRVDDAWRPHQVWRHEVGTA
                     TEADVLVYDEPDERFWVGLGSSRDDRWILLGSGSKTSSEVSILDAADPLGTSRVITSR
                     RDGIEYDVEVVGDRLFITHNGDHPDFDLAWAPLTCASASEWQPCRVRGEGERVVAVDA
                     FTDFAVVSLRSHGLTALRILPYDASGPAVFGAGWDVVPEEELYTIGLGANLESATRRL
                     QVVVESMVTPHSIYDYDVDERTWTLLKRQPVLGGYDPGAYRQHRAWVTIDSTTTWSRR
                     VADSRLAPSPIVYRSSSGTTSQPAPKTA"
BASE COUNT          530 a         1317 c         1253 g          591 t
ORIGIN      
        1 atctacctcg gcgagtgccg ccacatgggc atcaacgtgc tcccgcccga tgtcaactcc
       61 tcgatcgcca acttcgccgc ggtcggggcg gacatccggt tcgggctcgc cgcggtacgc
      121 aacgtcgggg tcaacgtcgt cgaggcgatc gcggtgacac gggcgcagaa gggtgccttc
      181 gcctccttcg aggacttcat ccgcaaggtg ccgatggtgg tctgcaacaa gcggactgtc
      241 gagtcgctca tcaaggcggg agcgttcgac tccctcggtg acacgcgctt cgggttgttc
      301 cgcgtgcacg aggcgtacgt cgacgccctc gtcgacgaga agcgtcagga agcgatcggg
      361 caggactccc tgttcggggg gttcggcgag gcaaccggcc cgggcgccgg tgatttcgtc
      421 gtcctgccga cgactccacg gaccgagtgg gacaagcagg cgctgctggc cttcgagcga
      481 gagatgctcg ggctctacgt ctcggaccat ccgctgctcg ggatcgagca catcctcgcc
      541 cagcacgccg acacctcgat cgtctcgctc ttcggcgacg agccgaagcc ggacggcacc
      601 tcggtgacca ttgccgggct catcaccggg ctgcagatca agcggacgaa gaagggcgac
      661 ctctgggccg tcgcgaccgt cgaggatctc gagggctcga tcgagtgcct cttcttcccg
      721 gcgtcgtaca tcgcggtgtc ggccatgctc ggccacgacg tcgtctgcgc cgtcaagggg
      781 cgggtcagct cacgcgacga cagcgtgtcg atcatcgccc aggacctcac cctgcccgac
      841 atcgtggagg ggccgcgcgg gccggttgtg ctcacgcttc ccgtcgcacg agccacctcg
      901 acggtggtgg agcggctgcg ccgggtcctg cgtgagcacc cgggggggac cgaggtccac
      961 gtgcggctcg tccagcccgg ccgctcgacg ctgatgaccc tcgatgacgc cttccgggtt
     1021 gccgcgagcc ccgcgttgta tggcgacctc aaggctctgc tcggtccggc ctgcctcggc
     1081 gaacgcgtgc cgtgagcccg ggaccggcat ccctggcgca cacccggtcc tcccggatgg
     1141 cgagcctggg gatcgcgcct cgacgcttca cctcccctgc ggcagtcgtg ctcgggctcg
     1201 gcgcggtcca ggcgcaggac tatgccgccg cttcgtgggc tctcgggctg cgctgcgcga
     1261 gcgggacatt ggccgatgtc gtgcaggcag taacagatcg cgaggtcgtg cggacctggc
     1321 cgatgcgagg gacgctgcat ctcgtaccgg ctgcggacgc cggctggatg tgccacctgc
     1381 tcaccggccc cccgatccgt gcggcggccc ggctgttcgc ccatctcggg ctgaccgagg
     1441 aggtgctcgg cactgcccga ggcgcgatcg aacgggcact caccggcggg gttgcgctca
     1501 gtcgtcctga gctgtttgcc ctcctcgccg gcctcggcat cgacccgtcg gcgcagcgcg
     1561 ggatccatct gctcggtcgc ttcagtcagc agggcctcct gtgccagggg gttccacagg
     1621 ggcgccagcc gaccttcaca ttgctcgagg agtgggtgcc ggggccgaac cgtcccaccc
     1681 gcgaggaggc cctggcaacc ctggctcggc gatatgtcgg cggccatggt ccggtgacag
     1741 aacgcgactt cgcgtggtgg accggtcaga ccctcggctt cgcccgtgag gcattcgggc
     1801 tgatcgacgc cgacgtggaa cgagtgtcgg tcgacgggcc accccatgtc gtccttcgcg
     1861 acgcgccaac ctcgttgccc cgtcacggag ctcatctgct gcccggctac gacgagctca
     1921 tcctgggcta tcaggaccgg tccgcgacga tggacgccgc caccctggct cgagtcgttc
     1981 ccggctccaa cggggtgttc cagccgacca ttctcgccaa cggccgggtc gtcggcacct
     2041 ggactcgcat gaccacgtcc agcggggtcg tcgtcacggc ccggcctttc gcaccgctgt
     2101 cccggacgcg gctcgcctcc gtgggccgcg cggccgcgtc atacgggcgc ttcctcggcg
     2161 cgatgatcag catgcggctc gagcggacct agcgcgcccc gcccccgtgt ggtcgcggtt
     2221 cgagacgggc gtccgccggg caagtaggct gcagccgtga tccagcctcc cgtcgccaaa
     2281 cgggtcaacc acatccgaga gcaccacggc gagcgggtcg tcgacgagta cgagtggctc
     2341 cgcgacgcca agaaccccga ggtcatcgcc cacctcacgg cggagaacag gtacgccgag
     2401 gagcggctcg cgcacctcgc gccgctgcgc gaggcgatct tccaggagat caaggggcgc
     2461 acccaggaga ccgacctctc ggtaccggtc gcgctcgacg gatggtggta ctacgccagg
     2521 tccgtgcagg gcaagcagta cgccctgcag tgccgtgcac cccgtcgccc gggcgagccg
     2581 cggccgcacc tgacgcctga gcagccggtc cccggcgagc agctcctcct cgacggcaac
     2641 gtcgaggcgg gggagagcga ctacttcgcg ctcggcgcga gtgaggtgtc catcgacggc
     2701 gacctcctcg ccttcgccgc cgaccacgtc ggcgacgagc ggttcgccgt gacggtgcgc
     2761 cggatcgcga ctggggagat catcgacacg gcagtgtccg acacgggtta cggcctggtc
     2821 tggagccttg acggccgcta tctcttctac acccgagtcg acgacgcctg gcgtcctcat
     2881 caggtctggc ggcacgaggt cgggacggcc accgaggccg atgtgctcgt ctatgacgag
     2941 cctgacgaac ggttctgggt cggtctcggg tcctcccgcg acgaccgctg gatcctgctc
     3001 ggcagcggct cgaagacgtc gagcgaggtc agcatcctgg acgccgccga cccgctcggg
     3061 acttcgcgtg tcatcacctc acgccgcgac gggatcgagt acgacgtcga ggtcgtcggc
     3121 gaccgcctgt tcatcaccca caacggcgac caccccgact tcgacctcgc ctgggcgccg
     3181 ttgacctgcg cgagcgcgag cgagtggcag ccgtgccgag tccgcggcga gggcgagcgc
     3241 gtcgttgccg tcgacgcgtt taccgacttc gcggtggtct ccttgcgcag ccatggactc
     3301 actgcgcttc ggatcctccc gtatgacgcg tccggcccgg ccgtcttcgg cgccggctgg
     3361 gatgtcgtcc ccgaggagga gctctacacg atcgggctcg gcgccaacct cgagtccgcg
     3421 acgcgtcggc tccaggtcgt cgtcgagtcg atggtgaccc ctcacagcat ctacgactac
     3481 gacgtcgacg agcgcacctg gaccctgctc aaacgccagc ccgttctcgg cggctacgac
     3541 cccggtgcct accggcagca ccgcgcgtgg gtcaccatcg actcgacgac gacctggagc
     3601 cgacgcgtcg cggactcgag gttggcaccg agcccgatcg tgtacaggtc ctcgtcgggg
     3661 acgacgtccc agccggcgcc gaagactgct g
//
LOCUS       DATAIX010000304         1785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_185985, whole genome shotgun sequence.
ACCESSION   DATAIX010000304 DATAIX010000000
VERSION     DATAIX010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185985"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1688)
                     /gene="glgX"
                     /locus_tag="VHM65_04515"
     CDS             complement(<1..1688)
                     /gene="glgX"
                     /locus_tag="VHM65_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX2704999.1"
                     /translation="MLTPSRRTRDLPPRPGVSLVEGGAEISVYAGHADAVEVCLFEPG
                     DSTGASERRIPLTDRAHGWWFGHLAGVGPGQRYGVRAHGPWQPPEGLRYNPQKLLLDP
                     YARALEGEVLWRPEVYGHVLGGEPGEDPSLRDDRDSAPYVPRGVVIDDRFTWGHDSSP
                     GHPVSASVLYEVHVRNITARMPGVPEHLRGTYAGLAHPATIEHLTRLGVTAVELLPIH
                     EFTHEPALVARGLRNHWGYNTLGFFAPRAAYAAARDPQGVLDEFKGMVKLLHAAGLEV
                     ILDVVYNHTCEQSHAGATLSWRGLDNRAYYRLDERGHDIDVTGCGNTLDLRHPVVVRM
                     VLDSLRYWAKECHVDGFRFDLAVALGRGRSDDFDPDHPFLVALQADPVLAEVGLIAEP
                     WDLGIHGWRTGQFPPPFSEWNDRYRDAVRTFWLPDLARDAVGQGGRGVRELATRLAGS
                     QDLFGAHDRGPTASVNFITAHDGFTLADLTAYNSKHNDANGEGNQDGSNDNHSWNHGI
                     EGPTDDPMVAGFRRRNVRNMLATLLVSTGVPMLCAGDEFGRTQRGSNNAYCQDN"
BASE COUNT          252 a          612 c          654 g          267 t
ORIGIN      
        1 tcgttgtcct ggcagtaggc gttgttggag ccgcgctggg tgcggccgaa ctcgtcaccg
       61 gcgcagagca tcgggacacc ggtcgacacg agcagggtcg ccagcatgtt gcggacgttg
      121 cgacggcgga acccggccac catcgggtcg tcggtgggcc cctcgatccc gtggttccac
      181 gagtggttgt cgttgctgcc gtcctggttg ccctcgccgt tggcgtcgtt gtgcttgctg
      241 ttgtaggccg tgaggtcggc cagcgtgaac ccgtcatggg cagtgatgaa gttgaccgag
      301 gccgtcggcc cgcggtcgtg cgccccgaag aggtcctgcg agccggccag gcgggtcgcc
      361 agctcgcgca cgccgcggcc accctggccg accgcgtcgc gggcgaggtc gggcagccag
      421 aaggtgcgta ccgcgtcgcg gtagcggtcg ttccactccg agaacggcgg cgggaactgc
      481 cccgtgcgcc agccatggat cccgaggtcc cacggctcgg cgatgaggcc cacctcggcg
      541 agcaccgggt cggcttgcag cgcgacgaga aacgggtggt cggggtcgaa gtcgtcgctg
      601 cgcccgcgcc ccagcgccac ggccaggtcg aagcggaacc cgtcgacgtg gcactccttg
      661 gcccagtagc gcagcgagtc gagcaccatg cgcaccacca ccggatggcg caggtcgagg
      721 gtgttgccgc agccggtgac gtcgatgtcg tggccgcgct cgtccaggcg gtagtaggcc
      781 cggttgtcca ggccccgcca cgacagggtc gctccggcgt gactctgctc gcaggtgtgg
      841 ttgtagacga cgtcgagaat gacctcaagt cccgcagcat ggagcagctt gaccatgccc
      901 ttgaactcgt cgaggacacc ctgggggtcg cgcgccgcgg cgtaggcggc tcgcggcgcg
      961 aagaacccga gcgtgttgta gccccagtgg ttgcgcagcc cgcgggcgac gagggccggc
     1021 tcgtgggtga actcgtggat cgggagcagc tcgaccgccg tgacgccgag ccgggtgagg
     1081 tgctcgatcg tcgcggggtg cgccagaccg gcatacgtcc cccgcaggtg ttccggcacg
     1141 ccgggcatcc gggcggtgat gttgcgcaca tggacctcgt agagcacgct cgccgacacc
     1201 ggatgcccag ggctgctgtc gtggccccag gtgaagcggt cgtcgatgac cacccctcga
     1261 ggcacgtatg gcgcgctgtc gcggtcgtcg cgcaggctcg ggtcctcgcc gggctcaccg
     1321 cccaggacgt gcccgtagac ctcgggccgc cacaggacct cgccctcgag ggcgcgggcg
     1381 taggggtcga ggagcagctt ttgcgggttg tagcgcaggc cttcgggggg ctgccacgga
     1441 ccgtgcgcac gcacgccata ccgctgcccg gggccgaccc cggcaaggtg accgaaccac
     1501 cagccgtggg cgcgatcggt caggggtatc cgccgctcgc tcgcgcccgt cgagtcgccc
     1561 ggctcgaaga ggcacacctc gacggcatcc gcgtgcccgg catacacgga gatctcggcg
     1621 cctccctcga ccaggctgac ccccggcctc gggggaaggt cgcgggtccg gcgcgatggc
     1681 gtgagcatga cgccaagcgt attggctcgt tacggtggag ccatgacgga ccccacctcc
     1741 ctgcccaact tcccaccgcc gccggaggaa cacccactgc gcggt
//
LOCUS       DATAIX010000305         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_275378, whole genome shotgun sequence.
ACCESSION   DATAIX010000305 DATAIX010000000
VERSION     DATAIX010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275378"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /gene="sufB"
                     /locus_tag="VHM65_04520"
     CDS             <1..807
                     /gene="sufB"
                     /locus_tag="VHM65_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX2705000.1"
                     /translation="LQAYFRINTENMGQFERTLIIADEGSYVHYVEGCTAPIYKSDSL
                     HSAVVEIIVKKNARVRYTTIQNWSNNVYNLVTKRATAGQGATMEWVDGNIGSKVTMKY
                     PAVYLLGEHAKGETLSIAFAGEGQHQDAGAKMVHAAPYTSSTIVSKSVARGGGRTSYR
                     GLVQVMEGAHHSRSTVRCDALLVDQISRSDTYPYVDVREDEVTMGHEATVSKIGDDQL
                     FYLMSRGMTEEEAMAMIVRGFVEPIARELPMEYALELNRLIELQMEGAVG"
     gene            807..1973
                     /gene="sufD"
                     /locus_tag="VHM65_04525"
     CDS             807..1973
                     /gene="sufD"
                     /locus_tag="VHM65_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX2705001.1"
                     /translation="MPLLQAQPHRHGVPSPAFVPNQSRAERTTSFDVADFPMPSGREE
                     EWRFAPVDTLGGLLADRPSDAHLAWETGLPAGVTLTEISTERARELGAQPPGDRAAVL
                     AVAHSGAAMLLEIPVEAEPDAPVRVRLTGHGRDVVRSHLVVAAGRHSKATVVLEHDGT
                     TALSEVVSVLVGDGADLTVVTVQGWDGDALHLAQHDATLGRDARYKHVCVTLGGKVVR
                     LNTIVRYAGPGASATLLGVYFADAGQHLEHRSFVDHSTAHCTSLVTYKGALQGDTAHT
                     VWVGDVLIRASAEGTDTYELNRNLVLTDGARADSVPNLEIETGEIARAGHASATGRFD
                     DEQLFYLQARGIPESEARRLVVRGFFVDVIAQIGVPDVQEQLMAAIEAELATAP"
     gene            1982..2326
                     /locus_tag="VHM65_04530"
     CDS             1982..2326
                     /locus_tag="VHM65_04530"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX2705002.1"
                     /translation="MTSYTLVCALDELSLDTPARAEVDGTVVALVRCLDASGTDVVYA
                     VDDRCTHANVSLSEGEVDHCQIECWLHGSRFDLSTGRPSGPPATIPVATYPVLVQDGS
                     VLVDVSAEPKES"
     gene            2333..>2496
                     /locus_tag="VHM65_04535"
     CDS             2333..>2496
                     /locus_tag="VHM65_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2705003.1"
                     /translation="MSTLQIRGLEVTVDVEGGVKEILRGVDLTVRGGETHAIMGPNGS
                     GKSTLAYSIAG"
BASE COUNT          404 a          912 c          829 g          351 t
ORIGIN      
        1 ctgcaggcct acttccggat caacaccgag aacatgggcc agttcgagcg gaccctgatc
       61 atcgccgacg aaggctccta cgtgcactac gtcgagggct gcaccgcccc gatctacaag
      121 tccgacagcc tgcacagcgc ggtcgtcgag atcatcgtca agaagaacgc ccgggtccgc
      181 tacacgacca tccagaactg gtccaacaac gtctacaacc tcgtcaccaa gcgggcgacc
      241 gcgggccagg gcgcgacgat ggagtgggtc gacggaaaca tcggctcgaa ggtgacgatg
      301 aagtacccgg ccgtctacct gctcggcgag catgccaagg gcgagaccct ctcgatcgcc
      361 ttcgccggcg agggccagca ccaggacgcc ggggccaaga tggtccacgc cgcgccctac
      421 acgagcagca ccatcgtgag caagtccgtc gcgcggggcg gcgggcgcac gtcataccgg
      481 ggcctggtcc aggtcatgga gggcgcgcac cacagccgct cgaccgtgcg ctgcgacgcc
      541 ctgctcgtcg accagatcag ccgctcggac acctacccgt acgtcgacgt ccgcgaggac
      601 gaggtcacga tgggtcacga ggccacggtc tccaagatcg gcgacgacca gctgttctac
      661 ctcatgtccc gcgggatgac cgaggaggag gccatggcga tgatcgtgcg cggcttcgtc
      721 gagccgatcg cccgggagct gcccatggag tacgccctcg agctcaaccg gctcatcgag
      781 ctgcagatgg aaggagcagt cggctgatgc cgctcctgca agcccagcca caccgtcacg
      841 gcgtcccctc cccggccttc gtccccaacc agtcgcgcgc ggagcggacg acctcgttcg
      901 acgtcgccga cttccccatg cccagcggac gcgaggagga gtggcgcttc gcccccgtcg
      961 acaccctggg cggactgctc gccgaccggc cgtcggacgc tcacctcgcg tgggagacgg
     1021 gcctgccggc tggtgtgacg ctcaccgaga tctcgacgga gcgcgctcgt gagctgggtg
     1081 cgcagccgcc gggggaccgt gcggccgtcc tcgcggtggc ccactcgggg gcggcgatgc
     1141 tgctcgagat cccggtcgag gccgagccgg acgccccggt ccgcgtgcgg ctcaccggcc
     1201 acgggcgcga cgtcgtccgg agccatctcg tcgtggcggc cgggcggcac agcaaggcca
     1261 cggtcgtcct cgagcacgac ggcaccaccg cgctgtccga ggtggtctcg gtcctcgtcg
     1321 gggacggggc cgacctcacc gtcgtcaccg tccagggatg ggacggcgat gcgctgcacc
     1381 tcgcccagca cgacgccacg ctcggccggg atgcccgcta caagcacgtc tgcgtcaccc
     1441 tcggcggcaa ggtcgtccgg ctcaacacca tcgtcaggta tgccggtccg ggcgcctccg
     1501 cgaccctgct cggcgtctac ttcgccgacg cgggacagca cttggagcac cgctccttcg
     1561 tcgaccacag caccgcgcac tgcacgtccc tggtcaccta caagggcgcg ctgcaagggg
     1621 acacggccca cacggtgtgg gtcggtgacg tcctgatccg ggccagcgcc gaggggaccg
     1681 acacctacga gctcaaccgc aacctcgtcc tcaccgacgg cgcccgggcc gactcggtgc
     1741 ccaacctcga gatcgagacc ggcgagatcg cccgagcggg gcacgcctcc gccaccgggc
     1801 gtttcgacga cgagcagctg ttctacctgc aggcccgggg catccccgag tccgaggcgc
     1861 ggcgccttgt cgtgcgcggc ttcttcgtcg acgtcatcgc ccagatcggt gtgcccgacg
     1921 tccaggagca gctcatggcg gccatcgagg ccgagctcgc gacggcgcca tgaggacccg
     1981 ggtgacgtca tacacgctcg tctgtgccct ggacgagctg agcctcgaca ccccggcgcg
     2041 ggccgaggtc gacgggacgg tcgtggccct cgtgcggtgc ctcgacgcct ccggcaccga
     2101 cgtcgtctat gcggtggacg accggtgcac ccacgccaac gtctcgctgt ccgagggcga
     2161 ggtcgaccac tgccagatcg agtgctggct gcacgggtct cgcttcgacc taagcaccgg
     2221 ccggccgtcc ggaccgcccg ccacgatccc cgtcgcgacc taccccgtcc tcgtgcagga
     2281 cgggtctgtg ctggtcgacg tttccgcaga gcccaaggag agctgaacac ctatgtcaac
     2341 gctgcagatt cgtggcctcg aggtcaccgt cgacgtcgaa ggtggcgtca aggagatcct
     2401 gcgcggggtc gacctcaccg tccgcggcgg cgagacccac gcgatcatgg ggcccaacgg
     2461 ctcggggaag tccacgctgg cctactcgat cgccgg
//
LOCUS       DATAIX010000306         2311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_165825, whole genome shotgun sequence.
ACCESSION   DATAIX010000306 DATAIX010000000
VERSION     DATAIX010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165825"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /gene="proB"
                     /locus_tag="VHM65_04540"
     CDS             complement(<1..979)
                     /gene="proB"
                     /locus_tag="VHM65_04540"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX2705004.1"
                     /translation="MSPRAPLTTAQRVVVKVGSSSLTRKGRLDTERLHALVAVIAARR
                     NQGTSVVLVSSGAIAAGLTPLHLTKRPRDLATQQAAASVGQGALIAAYQLAFAEHGLT
                     VGQVLLTAEDVTRRAHYVNARRTLDRLLDLGVVPVVNENDTVATSEIRFGDNDRLAAL
                     VAHLVAADALILLSDVDALYDGPPLRPGAQRIPVVRDVRDLDSVVIRGTGSHIGTGGM
                     ATKVEAAAIANAAGITTILTSAELAGQALSGQEDIGTVFVPTGPRRSSRLLWLAHATT
                     ARGRLILDAGAVAAVVDRRKSLLPAGITAVEGSFGDGEPVDICDPRGRVV"
     gene            complement(1007..>2311)
                     /gene="obgE"
                     /locus_tag="VHM65_04545"
     CDS             complement(1007..>2311)
                     /gene="obgE"
                     /locus_tag="VHM65_04545"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX2705005.1"
                     /translation="GAGDERSGADGDDLLLAVPDGTVVKDGSGRIVADLVGDGTAHVV
                     ARGGRGGLGNKALASPRRKAPGFALLGEPGEALDVVLELKTLADVALIGFPSAGKSSL
                     VSVLSAARPKIADYPFTTLVPNLGVVTAGDARFTIADVPGLIPGAHEGRGLGLDFLRH
                     IERCSVLVHVIDCATLETGRDPMSDLDVIEDELARYVPDADLGGRPLAERTRIVVLNK
                     ADVPEARDLAELVKPDLEGRGLEVFVVSAVARTGLRELTFALARQVGMARALAGSIVA
                     PRAVLRPRALDDSGFEVRVENTSDGPMYRIVGERPTRWVRQTDFSNDEAVGYLADRLN
                     RLGVEEELLKAGAVVGATVLIGAADDAVVFDWEPTMSTGAEFLGGPRGTDLRLGDGSR
                     PTREQKREAARDRYVARAATQAELDAERRAGHWATASDTDEA"
BASE COUNT          322 a          805 c          878 g          306 t
ORIGIN      
        1 cgaccacacg gccccgcggg tcgcagatgt cgaccggctc accgtcaccg aaggacccct
       61 cgacggccgt gatcccagcc gggagaagcg acttgcgccg gtcgacaacc gccgcgacag
      121 ccccggcgtc gaggatcagc cgcccgcggg cggtggtcgc gtgcgccagc cacaacagcc
      181 gtgacgaccg acgcggaccg gtcgggacga agaccgtccc gatgtcctcc tggccgctca
      241 gtgcctgccc ggcgagctcg gccgaagtga ggatcgtcgt gatcccggca gcgttggcga
      301 tcgccgcggc ctcgaccttg gtcgccatcc ctccggtccc gatgtgcgac ccggtccccc
      361 ggatcaccac gctgtccaga tcgcgcacgt cgcggacgac ggggatccgc tgcgcgcccg
      421 gacgcaacgg cggcccgtca tacagcgcat cgacgtccga gagcaggatg agcgcatcgg
      481 cggccacgag gtgcgcgacg agtgcggcga gccggtcgtt gtcaccgaaa cggatctcgc
      541 tcgtggcgac ggtgtcgttc tcgttgacga cggggacgac cccgaggtcg agcaggcggt
      601 cgagggtgcg ccgggcgttg acgtagtgcg cgcggcgggt cacgtcctcg gcggtgagca
      661 ggacctggcc gacggtgagc ccgtgctcgg caaaggccag ctggtatgcc gcgatgaggg
      721 ctccctgccc cacgctcgcg gccgcttgct gcgtcgcgag gtcccgtggg cgcttggtga
      781 ggtgcagcgg ggtcagtccg gcggcgatgg cgcccgagga gacgaggacg accgaggtgc
      841 cctggttgcg ccgggcggcg atgaccgcga cgagggcatg gaggcgctcg gtgtcgagcc
      901 gtcccttgcg ggtgagcgag ctggagccca ccttgacgac gacccgctga gccgtcgtca
      961 agggtgctcg gggggacatg gcgccaattc tagtgaggtg gggaccttat gcctcgtcgg
     1021 tgtccgacgc ggtcgcccag tgcccggccc ggcgttcggc gtcgagctcg gcctgggtgg
     1081 ccgcccgggc gacgtagcgg tcgcgtgccg cctcccgctt ctgttcccgg gtcgggcgcg
     1141 agccatcccc gagccgcagg tcggtgccgc gcggaccacc gaggaactcg gccccggtgg
     1201 acatggtcgg ctcccagtcg aagaccacag cgtcgtcggc agcgccgatg aggacggtgg
     1261 cgccgacgac cgcaccagcc ttgagcagct cctcctcgac gccgaggcgg ttcagccggt
     1321 cggcgaggta gccgacggct tcgtcgttgg agaagtcggt ctggcgcacc cagcgggtcg
     1381 ggcgctcccc gacgatgcgg tacatcggcc cgtccgaagt gttctccacc cggacctcga
     1441 agcccgagtc gtcgagggcc cgggggcgca gcacggcgcg cggcgccacg atcgacccgg
     1501 ccagggcccg cgccatcccg acctggcggg ccagggcgaa ggtgagctcg cgcagcccgg
     1561 tccgggcgac cgcggacacg acgaagacct cgagaccgcg cccttccagg tcgggcttga
     1621 cgagctcggc gagatcgcgg gcctcgggca cgtcggcctt gttgagcacg acgatccggg
     1681 ttcgctcggc caggggcctc cccccgaggt ccgcgtcggg cacgtagcgg gccagctcgt
     1741 cctcgatgac gtcgaggtcg ctcatcgggt cgcggccggt ctcgagggtc gcgcagtcga
     1801 tgacgtggac gagcaccgag cagcgctcga tgtgacgcag gaagtccagg cccaggccgc
     1861 gcccctcatg ggcgccgggg atgagccccg ggacgtcggc gatcgtgaac cgggcgtcac
     1921 cggcggtgac gacaccgagg ttgggcacga gggtcgtgaa cgggtagtcg gcgatcttgg
     1981 gccgggcggc ggagagcacc gacacgagcg aggacttgcc tgccgaaggg aagccgatca
     2041 gcgcgacgtc ggccagggtc ttgagctcga ggacgacatc gagggcctcc cctggttcgc
     2101 cgagcagggc gaagccgggc gccttgcgcc ggggcgaggc cagcgccttg ttgccgaggc
     2161 ccccgcggcc cccgcgagcc acgacgtgtg ccgtgccgtc gccgacgagg tcggcgacga
     2221 tccggccgga cccgtccttg acgacggtcc cgtcggggac cgcgaggagc aggtcgtcgc
     2281 cgtcggcgcc gctgcgctcg tcaccggcgc c
//
LOCUS       DATAIX010000307         2059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_186008, whole genome shotgun sequence.
ACCESSION   DATAIX010000307 DATAIX010000000
VERSION     DATAIX010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186008"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VHM65_04550"
     CDS             <1..592
                     /locus_tag="VHM65_04550"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX2705006.1"
                     /translation="QGFGPIPADLARDLLTGRDDIASTTDDGADANRDAASGAHTPPT
                     EKPDPDAPGPRPRTRLRRLFASTDRRRLVAMESARRTFPRSLAELIALRDQTCRTPYC
                     DAPIRHTDHVVPAARGGPTSEHNGQGLCEQCNYVKALPGWSARTEQPPGGRHQVILTT
                     PTGHTYTSTAPPPLGHGAIPRDESPLERRLRLLLAA"
     gene            complement(675..>2059)
                     /locus_tag="VHM65_04555"
     CDS             complement(675..>2059)
                     /locus_tag="VHM65_04555"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX2705007.1"
                     /translation="RAVVVLPLVPIIAVTGTNGKTTTARMIAHIFKGLGRKVGMTSTD
                     GIVIDERLAIKADASGPKSARMVLQNPRVDLAVMEVARGGILREGLGYDRNDVAVVTN
                     VAPDHLGLRGISTVEELANVKAVIVEAVPRDGFAVLNADDPLVRRMRRRCSGGIVWFS
                     LLPPGTRARDFIDDYCRRGGRAVVLDPTDKGDMIVIQHGRRRMQLAWTHLLPATFGGT
                     AKFNVANALAATGAAFAAGAPLHDIRQGLRTFTTSYYLSPGRMNLVTVNNVEVIVDYC
                     HNAPAMKVLGEFVDAYSDAKASRSELGRVSRIAVIATAGDRRDEDMRELGAVAARHFD
                     VLVVREDERPRGRKPGETAELVAAGVRAAIAEGARCRQVQIVLDELTAVRDCMTRANP
                     GDLVILCVDDHAVVMTELEERGRHAQAGSHSGPFVADPDLDPAGLSLTASHDVPEPAH
                     EAEQVTDQHA"
BASE COUNT          309 a          756 c          708 g          286 t
ORIGIN      
        1 gcagggcttc ggcccgatcc ctgccgacct ggcccgagac ctcctcaccg gtagagacga
       61 catcgcctcg accacggacg acggggccga cgccaaccgc gacgccgcgt ccggggcaca
      121 cacgcccccg accgagaagc ccgacccgga cgcaccgggc ccccggccgc gcacccggct
      181 gcgccggctc ttcgccagca ccgaccggcg ccgcctcgtg gccatggagt cggcccggcg
      241 caccttcccc aggtcgctgg ccgagctcat cgcgctccgc gaccagacct gccgcacgcc
      301 atactgcgat gcccccatcc gccacaccga ccatgtcgtc ccagccgccc gtggcgggcc
      361 gaccagcgaa cacaacggcc agggcctgtg cgagcagtgc aactacgtca aggcgctccc
      421 cggctggagt gcccggaccg aacagccccc cggcggacga caccaggtca tcctcaccac
      481 acccacgggc cacacctaca ccagcaccgc gcccccaccg ctcggccacg gagcgatccc
      541 gcgagacgag agccctctcg aacggcgcct gcgcctcctc cttgccgcgt aggcggatcg
      601 gcctagtgcc aatgtgtcca tggtggctgt ccgacgtagg gctgaagtcg ggttgcttaa
      661 ccgggctcga cccgtcaggc gtgctggtcg gtcacctgct cggcctcatg ggcaggctcc
      721 ggcacatcgt ggctggcggt caggctcagc ccggccgggt cgaggtcggg gtcggcaacg
      781 aagggcccgc tgtgcgaccc cgcctgggcg tgccggccgc gctcctcgag ctcggtcatg
      841 acgacggcgt ggtcgtcgac acacaggatc acgaggtcac cggggttggc gcgcgtcata
      901 cagtcacgaa ccgcggtcag ctcatcgagc acgatctgga cctggcggca gcgggccccc
      961 tcggcgatgg ccgcgcgcac gccggccgcg accagctcgg cggtctcgcc gggcttgcgg
     1021 ccacgcgggc gctcgtcctc gcggaccacg aggacgtcga agtggcgcgc tgccacggcg
     1081 ccgagctcgc gcatgtcctc gtcccggcgg tcgcccgccg tcgcgatgac ggcgatgcgc
     1141 gagacccggc cgagctcgga ccggctcgcc ttggcgtcgg agtaggcgtc gacgaactcg
     1201 ccgagcacct tcatcgccgg tgcgttgtgg cagtagtcga cgatgacctc gacgttgttg
     1261 acggtgacga ggttcatccg ccccggggag aggtagtagg aggtcgtgaa ggtccgcagc
     1321 ccctgacgta tgtcgtgcag cggcgcaccc gcggcgaagg ccgccccggt ggcggccagg
     1381 gcgttggcca cgttgaactt cgctgtccca ccgaaggtgg ccgggagcag atgggtccac
     1441 gcgagctgca tccggcgacg gccgtgctgg atgacgatca tgtcgccctt gtcggtgggg
     1501 tcgagcacga cggctcgccc accgcggcgg cagtagtcgt cgatgaagtc acgcgcccgc
     1561 gtgccgggcg gcagcaggga gaaccatacg atcccgccgg agcaccgccg tcgcatgcgg
     1621 cgcacgagcg ggtcatccgc gttgaggacg gcaaagccgt cacggggcac cgcttcgacg
     1681 atgacggcct tgacgttggc gagctcctcg acggtcgaga tcccccgcag gcccaggtgg
     1741 tcgggtgcga cgttggtcac gacagcgaca tcgttgcggt cgtagcccag gccctcgcgc
     1801 aggatcccac cgcgggccac ctccatcacc gcgaggtcga cgcgcgggtt ctgcaggacc
     1861 atccgggccg acttggggcc ggaggcgtcg gccttgatgg cgagccgctc gtcgatgacg
     1921 atgccgtcgg tcgacgtcat tccgaccttg cgtccgagtc ccttgaagat gtgggcgatc
     1981 atccgcgcag tcgtcgtctt gccgttggtg ccggtcaccg cgatgatcgg caccaacggc
     2041 aagacgacga ctgcgcgga
//
LOCUS       DATAIX010000308         1526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_16360, whole genome shotgun sequence.
ACCESSION   DATAIX010000308 DATAIX010000000
VERSION     DATAIX010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16360"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VHM65_04560"
     CDS             complement(<1..474)
                     /locus_tag="VHM65_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2705008.1"
                     /translation="MASPTPAERYAADRARAASRHLEDFVAGLDFPLDDFQRQACVAV
                     EAGHGVLVAAPTGAGKTVVGEFAVHLALATGTKAFYTTPIKALSNQKYTDLVRRHGAA
                     AVGLLTGDASVNGEAPVVVMTTEVLRNMMYAGSPTLGGLRYVVMDEVHYLADRFRG"
     gene            complement(484..1401)
                     /locus_tag="VHM65_04565"
     CDS             complement(484..1401)
                     /locus_tag="VHM65_04565"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX2705009.1"
                     /translation="MVERVGLLANPAAGAGRGAPIARTLARRLRTAGHDVHILSAPSL
                     ADGEAAARAAIDAGTIDVLFVAGGDGMVHAGANLCADTPVPLAVCAFGTGNDNARALG
                     IPIHEPQAAVALLRTGQIRRYDVGRRRAGGDGSRHTWLGVLGGGFDSVVNERANRWRW
                     PRGPMRYNLAMLRELPVFHPIPYRIEVDGHVIDAKAMLVAVGNGPAFGGGMQILPGAV
                     MDDGLLDVLILHEISIPAFLRVFPSVFTGQHVTHPSVQILRGRQVRLEAPGIMAYADG
                     ERFGALPLDLEVAPLVLQVVVPRAEHLPT"
BASE COUNT          229 a          519 c          572 g          206 t
ORIGIN      
        1 tccgcggaac cggtcggcga ggtagtggac ctcgtccatg acgacgtagc gcagcccccc
       61 gagggtgggc gacccggcat acatcatgtt gcgcaggacc tcggtcgtca tgacgacgac
      121 gggcgcctcg ccgttgaccg aggcgtcgcc ggtgagcagc ccgacggcgg cggcgccatg
      181 gcggcgcact aggtcggtgt acttctggtt cgacagggcc ttgatcgggg tcgtgtagaa
      241 cgccttggtc ccggtggcca gcgccagatg gacggcgaac tccccgacga cggtcttgcc
      301 ggcaccggtc ggggcggcga cgagaacgcc atggcccgcc tccactgcca cgcacgcctg
      361 gcgctggaag tcgtcgagcg ggaagtcaag gcctgcgacg aagtcctcga ggtgcctgct
      421 cgcggccctc gctcggtcgg cagcgtaccg ctcggcagga gtcgggctgg ccatgaggtc
      481 agactacgtg gggagatgct cagcccgcgg gacgacgacc tgcaggacca acggggcgac
      541 ctcgaggtcg agcggcagcg ccccgaaccg ctccccgtcg gcgtaggcca tgatgccggg
      601 ggcctcgagc ctgacctggc ggccgcgcag gatctggacc gacgggtggg tgacgtgctg
      661 gcccgtgaag accgacggga agacccgcag gaaggccggg atcgagatct cgtgcaggat
      721 gaggacgtcg agcagcccgt cgtccatgac ggccccgggc aggatctgca tcccaccgcc
      781 gaaggccggc ccgttgccga cagcgacgag catcgccttg gcatcgatga cgtggccgtc
      841 gacctcgatc cggtagggga tcgggtggaa gacgggcagc tcgcgcagca tcgcgaggtt
      901 gtagcgcatg gggccccgtg gccagcgcca gcggttggcc cgctcgttga cgacggagtc
      961 gaagcccccg ccgagaaccc cgagccaggt atgacgcgag ccgtcacctc ccgcgcggcg
     1021 gcgacccacg tcataccggc ggatctgccc cgtgcgcagg agcgcgacgg cagcctgcgg
     1081 ctcgtggatc gggatgccga gggcacgcgc attgtcgttg cccgtgccga aggcgcacac
     1141 cgcaagcggg acgggggtgt cggcgcacag gttcgcgccg gcatggacca tgccatcgcc
     1201 accggcgacg aagagcacgt cgatggtgcc ggcgtcgatg gcagccctcg cggcggcctc
     1261 gccgtcggcg aggcttggcg cgctgaggat gtggacgtca tgcccggccg tgcgcaggcg
     1321 ccgggcaagg gtccgggcga tcggcgcacc gcgtccagcc ccggctgccg ggttggccag
     1381 cagcccgacc cgctcgacca tgcgcctctc ccctcgctcg tcatccggct gcggcctcag
     1441 agggcggagg cctcctcgtc cgggacggcg agccactcgg ggtcgtgctt ggcgcgtcgt
     1501 cggtcaatga tggccgagat gccgac
//
LOCUS       DATAIX010000309         2393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_46643, whole genome shotgun sequence.
ACCESSION   DATAIX010000309 DATAIX010000000
VERSION     DATAIX010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46643"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VHM65_04570"
     CDS             <1..399
                     /locus_tag="VHM65_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705010.1"
                     /translation="VATATQLSRTAETLRDHGGFTEPQAWESLVRGWHLEGLAVRPEH
                     RMHGHTGFLISARRLAPGVRTPRRTRRPAKGAYDEQAAGTRHTDEVWGAEDLGEQPKS
                     ERAIRRARRSVGDTSPTPGAGRARVEDEPV"
     gene            433..2193
                     /gene="arc"
                     /locus_tag="VHM65_04575"
     CDS             433..2193
                     /gene="arc"
                     /locus_tag="VHM65_04575"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HEX2705011.1"
                     /translation="MGSDPTRDDPSGPVADAGSESQDRAALAAEVAELRERMRHSPTR
                     TRELENRIISLQTSISTLSAQNDRLVRTLKDAREQIVGLRAEVDRLAQPPSSYGIILE
                     SYDDATVDVITSGRKMRVAVSPAVDPSDLATGREVRLNEALNVVQTCGFERVGEVVSI
                     KEVLSAERVLVVAHADEERVCRIAQSLDGIKIRVGDALLLDPRSGFVFELIPKAEVEE
                     LVLEEIPSIAYEDIGGLAGQIEQIRDAIELPYLHPDLFSEHKLRPPKGVLLYGPPGCG
                     KTLIAKAVAASLAKKVTERTGQSMEKSYFLNIKGPELLNKYVGETERHIRLIFQRARE
                     KASDGTPVVVFFDEMDSLFRTRGSGVSSDVETTIVPQLLSEIDGVERLENVIVIGASN
                     REDMIDPAILRPGRLDVKIKIERPDAEAARDIFTKYLTADLPLHEHDLAEHGHDRDRT
                     VDVMIAAAVERMYSEIDQNRFLEVTYAGGDKEILYFKDFNSGAMIQNIVDRAKKMAIK
                     DFLTTGSKGLRVEHLLSSCLDEFKENEDLPNTTNPDDWARISGKKGERIVYIRTLVTG
                     KDGTEPGRSIATVANTGQYL"
BASE COUNT          414 a          835 c          825 g          319 t
ORIGIN      
        1 gtcgccacgg cgacccagct ctcccgcacc gccgagaccc tgcgtgacca cggcggcttc
       61 accgagccgc aggcgtggga gtcccttgtc cgggggtggc atctggaggg cctggcggtc
      121 cggcccgagc accggatgca cggtcatacc ggcttcctca tcagcgcccg gcgcctcgcc
      181 ccgggcgtac ggaccccacg gcggacccgt cgccccgcca agggtgcgta tgacgagcag
      241 gcggcgggca cgcgtcatac cgacgaggtc tggggggccg aagacctggg cgagcagccc
      301 aagtccgaac gcgcgatccg acgcgcccga cgttcggtgg gcgatacctc tcccactccc
      361 ggggccgggc gagctagggt cgaagacgag ccggtctgat tctggctgcc cgcccgagca
      421 acggaggtgc acatgggaag tgacccgacc cgcgacgacc cttccgggcc cgtggccgac
      481 gcaggcagcg agagccagga ccgggccgcc ctcgctgcgg aggtcgcgga gctgcgtgag
      541 cggatgcgcc actcaccgac ccgtacccgc gagctggaga accgcatcat ctccctgcag
      601 acgtcgatct cgacgctgtc cgcccagaac gaccggctcg tgcgcacgct caaggacgcg
      661 cgcgagcaga tcgtgggcct gcgggccgag gtcgaccggc tcgcgcagcc gcccagcagc
      721 tacggcatca ttctcgagtc gtatgacgac gccaccgtcg acgtgatcac cagcggccgc
      781 aagatgcgcg tcgcggtgag cccggcggtc gacccgtcgg acctggccac cgggcgcgag
      841 gtgcggctca acgaggccct caacgtcgtg cagacctgcg gcttcgagcg ggtgggcgag
      901 gtcgtctcga tcaaggaggt cctctcggcc gagcgggtgc tcgtcgtcgc gcacgccgac
      961 gaggagcggg tctgccggat cgcacagtca ctcgacggca tcaagatccg ggtcggggac
     1021 gccctgctgc tcgacccgcg ctccgggttc gtcttcgagc tgatccccaa ggccgaggtc
     1081 gaggagctcg tgctcgagga gatccccagc attgcctacg aggacatcgg gggcctggcg
     1141 ggccagatcg agcagatccg cgacgcgatc gagctgccct acctgcatcc ggacctgttc
     1201 agcgagcaca agctgcgccc acccaagggc gtgctgctct atggtccgcc cggctgcggc
     1261 aagaccctca tcgccaaggc cgtcgcggcc tcgctggcca agaaggtgac cgaacgcacc
     1321 gggcagtcga tggagaagag ctacttcctc aacatcaagg gacccgagct gctcaacaag
     1381 tacgtcggcg agaccgagcg ccacatccgg ctcatcttcc agcgggctcg ggagaaggcg
     1441 tcggacggga ccccggtcgt cgtcttcttc gacgagatgg actcgctctt tcgcacccgc
     1501 gggtccgggg tgtccagcga cgtcgagacg acgatcgtgc cgcagctgct ctcggagatc
     1561 gacggggtgg agcggctcga gaacgtcatc gtcatcggcg cctcgaaccg tgaggacatg
     1621 atcgacccgg cgatcctgcg accgggccgg ctcgatgtca agatcaagat cgagcgcccg
     1681 gatgccgagg ccgcacggga catcttcacg aagtacctca cggccgacct gccgctgcac
     1741 gagcacgacc tcgccgagca cggccacgac cgggaccgca cggtcgacgt catgatcgcg
     1801 gcggccgtcg agcggatgta ctccgagatc gaccagaacc gcttcctcga ggtcacctac
     1861 gccggaggtg acaaggagat cctctacttc aaggacttca actccggggc gatgatccag
     1921 aacatcgtgg accgggccaa gaagatggcg atcaaggact tcctcacgac ggggagcaag
     1981 ggacttcgag tcgagcacct gctctcctcc tgcctggacg agttcaagga gaacgaggac
     2041 ctgcccaaca cgaccaaccc tgacgactgg gcccggatct ccggcaagaa gggcgagcgc
     2101 atcgtctaca tccgcaccct ggtcaccggc aaggacggga ccgagccggg gcgctcgatc
     2161 gccacggtcg ccaacacggg gcagtacctc tgacccagcc tggcagccgg gggggtcagg
     2221 cggggcgcgg atcgcggacc cggcgcgggc caccgaggtg ggcctcgtgc tgaccggcgg
     2281 ggccctggcc accaacgccc agctcggcga ggccgacggc ggctggggca tccaggggga
     2341 cccggccgag ggcgccttcc tcgtggcggc gcgcgcaacc cgtcgtctgg ggg
//
LOCUS       DATAIX010000310         4091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_16383, whole genome shotgun sequence.
ACCESSION   DATAIX010000310 DATAIX010000000
VERSION     DATAIX010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16383"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VHM65_04580"
     CDS             complement(<1..232)
                     /locus_tag="VHM65_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX2705012.1"
                     /translation="MARYTGPDCRRCRREKTKLFLKGAKCDSPKCPIELRPYPPGVHG
                     RARTKDSEYLLQMREKQKAARIYGVLEKQFRGY"
     gene            complement(262..669)
                     /gene="rpsK"
                     /locus_tag="VHM65_04585"
     CDS             complement(262..669)
                     /gene="rpsK"
                     /locus_tag="VHM65_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX2705013.1"
                     /translation="MPPKSRPAAGAKKVRRKEKKNVAHGHAHIKSTFNNTIISITDPN
                     GAVISWASAGQVGFKGSRKSTPFAAQMAAEAAARRAMEHGMRKVDVFVKGPGSGRETA
                     IRSLTATGLEVGAISDVTPTPHNGVRPSKRRRV"
     gene            complement(766..1143)
                     /gene="rpsM"
                     /locus_tag="VHM65_04590"
     CDS             complement(766..1143)
                     /gene="rpsM"
                     /locus_tag="VHM65_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016637793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX2705014.1"
                     /translation="MARLIGVDLPREKRVEVGLTYIFGVGRTRANQALAATGVSPDTR
                     VRDLTDEDLVKLRDYLEANVKMEGDLRREIAADIRRKVEIGTYEGLRHRRGLPVRGQR
                     TKTNARTRKGPKRTVAGKKKVGK"
     gene            complement(1395..1508)
                     /gene="rpmJ"
                     /locus_tag="VHM65_04595"
     CDS             complement(1395..1508)
                     /gene="rpmJ"
                     /locus_tag="VHM65_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX2705015.1"
                     /translation="MKVQPSVKKVCDKCKVIRRHGRVMVICENLRHKQRQG"
     gene            complement(1638..1865)
                     /gene="infA"
                     /locus_tag="VHM65_04600"
     CDS             complement(1638..1865)
                     /gene="infA"
                     /locus_tag="VHM65_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX2705016.1"
                     /translation="MAKKDGVIEVEGTVVEALPNAMFRVELSNPAGHRVLAHISGKMR
                     QHYIRILPEDRVVVELSPYDLSRGRIVFRYR"
     gene            complement(2054..2896)
                     /gene="map"
                     /locus_tag="VHM65_04605"
     CDS             complement(2054..2896)
                     /gene="map"
                     /locus_tag="VHM65_04605"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX2705017.1"
                     /translation="MFSRKTPGGKAPEEILLMRQAGLLVGRALELVSQSARAGMTTLD
                     LDEMAEDLIRTSGGVPSFQEVPGYRHTLCTSVNEEVVHGIPGPRVLRDGDLLSIDCGA
                     IVSGWHGDAAVTLVVGGLGAGRREDLLLSDATEDAMWAGIAALKVGERLYDVGAAVEG
                     SVVAAGERIGADYGIVQDYVGHAIGRQMHLDPQIPNYRVAQKGPILRDGFTGAIEPMV
                     TLGSPGTRVLADDWTVVTTDGLRAAHWEHTALVREEGLWVLTALDGGQARLGALGAPY
                     APMP"
     gene            complement(2911..3483)
                     /locus_tag="VHM65_04610"
     CDS             complement(2911..3483)
                     /locus_tag="VHM65_04610"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF011105.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX2705018.1"
                     /translation="MRLIILGAPGAGKGTQAARIAAHYGIPAISTGDIFRSNIKNGTE
                     LGKEVEAILAAGGYVPDEVTNSIVADRLSWADVEPGFLLDGYPRTVGQVEALDVLLAQ
                     QGHPLDAVLELTVDEEAVVERLLLRAAEQGRADDSESVIRERLAIYHAETAPLSAIYQ
                     DHHVLVEVDGMGEVDDVTERIYAALPSAVP"
     gene            complement(3483..>4091)
                     /locus_tag="VHM65_04615"
     CDS             complement(3483..>4091)
                     /locus_tag="VHM65_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HEX2705019.1"
                     /translation="EQSQRRVPVQYAKRMVGRRMYGGSATYIPLKVNMAGVIPVIFAS
                     SMLALPGLIAQFNQRADGKNAAWVTWILANLTRGSEGPYMVAYIGFIIFFTFFYVSIT
                     FNPVEVADNMKKYGGFIPGIRAGRPTAEYLSYVLTRITVPGAIYLGLISLIPLVALVL
                     IGADQNFPFGGTSILIIVGVGLETVKQIQAQLQQRNYEGFLR"
BASE COUNT          601 a         1354 c         1420 g          716 t
ORIGIN      
        1 agtaaccgcg gaactgcttc tccaggacgc cgtagatgcg ggcggccttc tgcttctcac
       61 gcatctgaag caggtactcg ctgtccttgg tccgcgcacg accgtgcacg cccgggggat
      121 acgggcgaag ctcgatcggg cacttcgggg agtcgcactt ggctcccttg aggaacagct
      181 tggtcttctc gcgccggcag cgcctgcagt caggcccggt ataacgcgcc ataagtattt
      241 atctcctctt ggtgcgcgtg gtcagacgcg gcgacgcttg gaagggcgga cgccgttgtg
      301 cggcgttggg gtgacatcgc tgatcgcgcc gacctcgagg ccggtggcag tcagcgaccg
      361 gatggcggtc tcgcgaccag aacccggacc cttgacgaag acgtcgacct tgcgcatgcc
      421 gtgctccatg gcgcgccggg ccgcagcctc ggccgccatc tgcgcggcga acggggtgga
      481 cttgcgtgag cccttgaagc cgacctgccc ggccgaggcc cacgagatca cggcaccgtt
      541 cgggtcggtg atcgagatga tcgtgttgtt gaaggtgctc ttgatgtgcg catgaccgtg
      601 ggcgacgttc ttcttctcct tgcggcgcac cttcttggcg cctgcagccg gtcggctctt
      661 gggaggcatc tgggattctt ctctttccta cgaataaacg ggtgaacagc gctccggtcg
      721 gcgcctccgc ggtccctcgg gcccacgggg ctagcccccg gctggttact tgccgacctt
      781 cttcttgccg gcgacggtgc gcttcgggcc cttgcgggtg cgcgcgttgg tcttggtccg
      841 ctgaccgcgc accgggagtc cacggcggtg ccgcaggccc tcgtaggtgc cgatctcgac
      901 cttgcggcgg atgtcagcgg cgatctcccg gcggaggtca ccttccatct tgacgttcgc
      961 ctcgaggtag tcacggagct tgacgaggtc ctcgtccgtg aggtcgcgca cacgggtgtc
     1021 cgggctgacg cccgttgccg ccagagcttg gttcgcgcgg gtgcgcccca cgccgaagat
     1081 ataggtcagg ccgacctcga cgcgcttctc gcgcggaagg tcgacgccaa tcagacgggc
     1141 catctgtgaa tctcgtctcc tgtaaccgtc tcggaggtct ggtgcagcac cgatccgggg
     1201 ttcctggccg acccgagact cgggcgggcc aaccggtccc cggcctccga gccgggggtg
     1261 acgtcctgcg gcggctcgac gagccggtca tcaggggccg ggtgctgctg ttctgttgtg
     1321 tcgtgcgtcg ctggtgccgg cgtagtgccg gcgtcggcgc gtcgtgcgag gctgtcacca
     1381 gcccggtgtc ctgcctagcc ctgacgctgc ttgtggcgca ggttctcgca gatcaccatg
     1441 acccgaccgt gacggcggat caccttgcac ttgtcgcaga ccttcttgac gctgggctga
     1501 accttcatct gcctgcgcat ttcctcgtcg tgcgccaggg cgggatggcc actgaggtgc
     1561 cagccctgat cctggagcgg gtgggatcgg gaggtgctga cgtcacgagc gagcccgggg
     1621 cgtcggcggt ggtgcggtca gcggtagcgg aacacgatgc ggccacgaga caggtcatag
     1681 ggcgagagct cgaccacgac ccggtcctca gggaggatcc ggatgtagtg ctgacgcatc
     1741 ttgcccgaga tgtgggcgag gaccctatgt ccagccgggt tgctcagctc cacgcgaaac
     1801 atcgcgttcg gcagcgcctc cacgactgtg ccctcgacct cgatgacacc gtccttctta
     1861 gccatatcct ccgctatccc tgtgcgcgca cgtcgggaac cgccatgagc gacggtcccc
     1921 cggtgaccgg tctcgggcgc gcctcaacgc acacgggacc gacgcaccag cctacgcgat
     1981 cggtccacga tccgggaaat ccgccgacga gcgctccacc cggcccgatc gtccccgcgt
     2041 ggcgcccgcg aggtcagggc attggggcgt atggcgcgcc gagggcaccc aggcgggcct
     2101 gtccgccgtc gagcgcggtg agcacccaga gtccctcctc gcgcacgagg gcggtgtgct
     2161 cccagtgcgc ggcccgtaac ccgtcggtcg tcacgaccgt ccagtcgtcg gcgagcaccc
     2221 gggtgccggg tgaaccaagg gtcaccatgg gctcgatcgc cccggtgaag ccgtcccgca
     2281 ggatcgggcc cttctgggcc acccggtagt tcgggatctg cgggtcgagg tgcatctgcc
     2341 gaccgatcgc gtggccgacg tagtcctgca cgatgccgta gtcggccccg atgcgctccc
     2401 cggcggccac gaccgagccc tcgaccgctg cgccgacgtc atagaggcgt tcgccgacct
     2461 tgagggccgc gatcccggcc cacatcgcgt cctcggtggc gtcggacagg agcaggtcct
     2521 ccctgcggcc ggcccccagg ccgccgacga cgagggtcac ggcagcgtcg ccgtgccagc
     2581 cgctcacgat ggccccgcag tcgatgctga gcaggtcccc gtcgcgcagg acccgcgggc
     2641 ccgggatgcc gtggacgacc tcctcgttga ccgacgtgca cagggtatgg cggtagcccg
     2701 ggacctcctg gaaggagggc actcccccgc tggtgcggat gaggtcctcc gccatctcgt
     2761 cgaggtcgag agtcgtcatc cccgcgcggg cgctctgcga gaccagctcc agggcccggc
     2821 cgacaagcag gcccgcctga cgcatgagca ggatctcctc gggggccttc ccgccaggtg
     2881 tcttgcggct gaacatcctg tccggcccgg tcagggcaca gccgaaggca gcgcggcgta
     2941 gatccgctcg gtgacgtcgt cgacctcacc catgccgtca acctcgacga ggacgtggtg
     3001 gtcctggtag atcgccgaga gtggagccgt ctccgcgtgg tagatcgcga gtcgctcgcg
     3061 gatgaccgat tcgctgtcgt cggcccggcc ttgctcggcg gcccgcaaca ggaggcgttc
     3121 gaccaccgcc tcctcgtcga cggtcagctc gagcacggcg tcgagcgggt gcccctgctg
     3181 ggccaggagg acgtcgaggg cctcgacctg gcctaccgtg cggggatagc cgtccagcag
     3241 gaagcccggc tcgacgtcgg cccagctgag ccggtccgcg acgatcgagt tggtcacctc
     3301 gtcggggaca taccccccgg cggcgaggat ggcctcgacc tccttgccga gttcggtgcc
     3361 gttcttgatg ttggagcgga agatgtcccc ggtcgagatc gcagggatgc cgtagtgcgc
     3421 ggcaatacgg gccgcttgcg tgcccttgcc ggctccgggt gcgcccagga tgatgagtct
     3481 catcagcgta gaaacccttc gtagttccgc tgctggagct gggcctggat ctgtttgacc
     3541 gtctcgaggc cgacaccgac gatgatgagg atcgaggtgc cgccgaaggg gaagttctgg
     3601 tcggcgccga tgaggacgag ggcgaccagc gggatcagcg agatgaggcc cagatagatt
     3661 gcgcccggga cggtgatccg ggtgagaacg tagctgaggt actcggccgt gggtcgcccg
     3721 gcgcgaatcc ctgggatgaa gccgccgtac ttcttcatgt tgtcggcaac ttcgacgggg
     3781 ttgaacgtga tcgacacgta gaagaacgtg aagaagatga tgaaaccgat gtaggcgacc
     3841 atgtagggcc cctcgctgcc ccgggtgagg ttcgccagga tccaggtgac ccaggcggcg
     3901 ttcttgccgt cggcgcgctg gttgaactgg gcgatgagcc ccggcaacgc gagcatcgac
     3961 gaggcgaaga tcacggggat gacgccggcc atgttgacct tgagcgggat gtacgtcgcg
     4021 ctcccgccat acatgcgccg accgaccatt cgtttggcgt actgaacggg gacccggcgc
     4081 tgggactgct c
//
LOCUS       DATAIX010000311         1750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_76528, whole genome shotgun sequence.
ACCESSION   DATAIX010000311 DATAIX010000000
VERSION     DATAIX010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76528"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VHM65_04620"
     CDS             complement(<1..339)
                     /locus_tag="VHM65_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705020.1"
                     /translation="MTTPAQRPWARVRYDRALVVVAALCWVLSLVRSWGAPSWTDRID
                     LIIIGAFALAVGLGEMVRLPMPGGRESAPLSAAAGLGFAMVPMFPDGVVPLYGGRQRG
                     AFPASGHREAH"
     gene            complement(336..>1750)
                     /locus_tag="VHM65_04625"
     CDS             complement(336..>1750)
                     /locus_tag="VHM65_04625"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX2705021.1"
                     /translation="TKATARAASPLRVPTIVYISAVCVAAVGLFLLAWRVAGPPRDWV
                     AVVVLTALVLIGHVFRERDVGSRVSISFASIVLLASVAIVGPVAAGAISVASIALDLR
                     SYPLRVTAFNAGMNATYSLLGALAYVWVGGRLDVASVDTVAALFSDVGLPLIAADAAQ
                     MVANAGILAGIMHIDGGMSYRSFLVSTLRTTGPAYFGYGIFGFLFALLWIPAGVGPFS
                     SVLIVAPLVVARWAFTQYGDELRAHERTLSSLVTSVETKNPFAAGHGERVAAMAQRIG
                     EVLTLGGPQLHALRYAAMLHDVGTISIPSRFLLSPGPLTEEAIGLIEAHPAQGVSVIS
                     NVDFLRPSLEGVRHHHERFDGSGYPDGLVGEQIPLFARVVAVADTFDSLTITRESRPA
                     HTVSEALDMVLARSGSQFDPQIVRALQEALDRQPWTPATPPARGLVGAEDFFDHDDPD
                     VSDLLADRRRDLCPRPPGHS"
BASE COUNT          315 a          576 c          648 g          211 t
ORIGIN      
        1 gtgcgcctcc cgatgcccgg aggccgggaa agcgccccgc tgtcggccgc cgtacaaggg
       61 aacgacgccg tcggggaaca tcggaaccat ggcgaacccc aatccggcgg cggccgacag
      121 cggggcgctt tcccggcctc cgggcatcgg gaggcgcacc atctcgccca ggccgacggc
      181 aagggcgaag gcgccgatga tgatcaggtc gatccggtcg gtccagctcg gggcacccca
      241 cgaacggacc aagctcagca cccagcacag cgccgccacg acaaccagcg cccggtcgta
      301 ccgaacccgg gcccagggtc gctgggcggg cgtcgtcacg agtggcccgg tggccgtggg
      361 cagaggtctc ggcgccggtc ggccagcaga tcggagacgt ccgggtcgtc gtggtcgaag
      421 aagtcctcgg caccgaccag gccgcgtgcg ggaggcgtgg cgggcgtcca gggctggcgg
      481 tcgagggcct cctgcagcgc ccggacgatc tgcgggtcga actggctgcc ggagcgcgcg
      541 aggaccatgt cgagtgcttc ggagaccgtg tgggccggcc gggactcgcg cgtgatcgtc
      601 agcgagtcga aggtgtccgc aacggcgacg accctggcga acaaggggat ctgctccccg
      661 actagcccgt cggggtaacc actcccgtcg aagcgctcgt gatggtgacg caccccctcg
      721 aggctgggcc tgaggaagtc cacgttgctg atgaccgaga cgccctgcgc cgggtgggcc
      781 tcgatgagcc cgatagcctc ctcggtcagg ggtcccggcg agagcaggaa gcggctgggg
      841 atgctgatcg tgccgacgtc gtgcagcatg gccgcatacc gcagggcatg cagctggggg
      901 ccgccgagcg tcagcacctc accgatccgc tgggccatgg cggccacgcg ttcgccgtgt
      961 ccggccgcga aggggttctt ggtctcgacg gaggtgacca gggagctgag ggtgcgctcg
     1021 tgcgcccgca actcgtcgcc gtactgggtg aacgcccacc gggcgacgac gagtggcgcg
     1081 acgatgagca ccgagctgaa cggtccgacc cccgcgggga tccacagcag ggcgaagagg
     1141 aagccgaaga tgccgtaccc gaagtaggcg ggcccggtgg tgcgaagcgt gctcacgagg
     1201 aaggaacggt aggacattcc cccgtcgatg tgcatgatgc ccgccagaat cccggcgttg
     1261 gccaccatct gggccgcgtc tgccgcgatg agcggtaagc cgacgtcgga gaacagggca
     1321 gcgacggtgt cgaccgaggc gacgtcgagg cggcccccga cccagacgta ggccagggcc
     1381 ccgagcagcg agtaggtcgc gttcatcccg gcgttgaacg cggtgacccg gagcgggtag
     1441 ctgcgcaggt cgagcgcaat ggaagcgaca gagatggccc ccgctgcgac cggtccaacg
     1501 atcgctacgg aagccaggag cacaatgctc gcgaaggaga tcgagactcg ggatccgaca
     1561 tcgcgttcgc gaaacacatg gccgatcagg acgagcgctg taaggaccac gacggccacc
     1621 caatcccggg gggggccagc gacccgccag gcgaggagga acaggccgac cgcagcgaca
     1681 cacaccgccg agatatagac gatggtcggc acccgcagcg gcgacgcggc tcgcgcggtc
     1741 gccttagtca
//
LOCUS       DATAIX010000312         2494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_245620, whole genome shotgun sequence.
ACCESSION   DATAIX010000312 DATAIX010000000
VERSION     DATAIX010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245620"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VHM65_04630"
     CDS             complement(<1..414)
                     /locus_tag="VHM65_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705022.1"
                     /translation="MTTGPDDPHDRHDPHDRHDPHVLLGGFVLGGLSDADHRTFTEHL
                     RTCGQCQREAGQLSGIPRLLDLVGPDGLPEPEPSRVADLLTALARERRRQRVRLAVAA
                     SAVAVLCLGAGALLGPRLGRSAEAPTQRYAAVAASG"
     gene            complement(411..911)
                     /locus_tag="VHM65_04635"
     CDS             complement(411..911)
                     /locus_tag="VHM65_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2705023.1"
                     /translation="MPLDDEAVAQIYREHGPMLRRLVWRVTGDVGRADDIVQEVILRV
                     WRKAPDPQNLRGYLVAAVRNLVVDQHRAAGRRPHEVAFAEPRTAEPAEPGDLDRALDQ
                     ILVEEGLGRLQGDHRVIVRALYFERLTVAETAARLGIPEGTVKSRAFYAVRSLRVILD
                     EMGVTR"
     gene            1081..1596
                     /locus_tag="VHM65_04640"
     CDS             1081..1596
                     /locus_tag="VHM65_04640"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705024.1"
                     /translation="MHPIRTLAPLACFVLLAGCAASGSTGGDDGYGAPAAASSSSSTT
                     ALPTPVTAAAITELKTAATSLGQVVTDGAGMTLYMFTKDTQGASASACAGACLAAWPQ
                     VVAGSTVPTLTGVSGAVAMIATADGRRQVTLAGWPLYYFAKDKAPGDVLGQGVNQVWY
                     VLDQAGTPVKK"
     gene            1687..2352
                     /locus_tag="VHM65_04645"
     CDS             1687..2352
                     /locus_tag="VHM65_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2705025.1"
                     /translation="MSRGPVQGSSGVTLTATGVSKEISGLPLLAPVDLIARPGECVVL
                     RGKNGSGKTTLLRILAGTLEPSAGRLDIDGQPVDERARHTRETVAALVGAIATYRDLT
                     LWDHLVLVESTWGLDPDTTEDRVQAMLELLEIDHLDDRFPHELSSGQQQLFHLALVLI
                     RPARLLLLDEPEQRLDTDKRRLLAQILAARAAQGTTVVMACHDPVVTEAVADRILDLE
                     PAS"
BASE COUNT          350 a          900 c          888 g          356 t
ORIGIN      
        1 gcccgaggcc gcgacggcgg cataccgctg cgtcggcgcc tcggccgacc gcccaagtcg
       61 agggccgagc agggcaccgg caccgaggca caggacggcg accgcactcg cggcgacggc
      121 gagacgcacc cgctgccgcc ggcgctcacg cgccagggcc gtgagcaggt cggcgacccg
      181 cgacggctcg ggctcgggca gtccgtccgg tccgacgagg tcgagcagcc tcgggatgcc
      241 gctgagctgg ccggcctccc gctggcactg gccgcaggtg cgcaggtgct cggtgaaggt
      301 gcggtggtcc gcatccgaga ggccgccgag gacgaagccg ccgagcagga cgtgcgggtc
      361 atgccggtcg tgcgggtcat gccggtcgtg cgggtcgtcg ggccccgtgg tcatcgggtc
      421 actcccatct cgtcgaggat gacgcgcaga ctgcggacgg cgtagaaggc ccgggacttg
      481 accgtcccct cggggatccc gagccgcgcc gccgtctcgg ccacggtgag gcgctcgaag
      541 tacagggccc gcacaatcac gcgatggtcg ccctggagcc ggcccagccc ctcctcgacg
      601 aggatctggt cgagcgcgcg gtcgagatct cccggctcgg cgggctcggc agtccggggt
      661 tcggcgaagg ccacctcgtg gggccggcgc ccggctgccc gatgctggtc gacgacgagg
      721 ttgcgcacgg cggcgacgag gtagccgcgc aggttctgcg ggtccggtgc cttgcgccag
      781 acccgcagga tgacctcctg gacgatgtcg tcggcgcgtc ccacgtctcc ggtgacccgc
      841 cagacgagcc ggcgcagcat cggcccgtgc tcacggtaga tctgcgcgac agcctcgtca
      901 tccagcggca tgggccgacc tccagtgctg cgttcgtggc atgcgtgttg tcgaaggaga
      961 cgtcgacgac gcccggctgg ttcagtgaac cgtctcacgg atgcggtcgt cttccttcat
     1021 gacactgcct tccttcatga cactggtgaa ccgtcatcgg tgcacagccg aggagtcccc
     1081 atgcacccga tccgcaccct cgcccctctc gcctgtttcg tcctgctcgc cggctgcgct
     1141 gcgtccgggt cgacgggagg cgatgacggg tatggcgccc ctgctgctgc gtcgtcctcg
     1201 tcgagcacga cggccctgcc caccccggtg acggcagcgg cgatcaccga gctcaagacc
     1261 gcggcgacct cgctcggaca ggtcgtgacc gacggtgcgg gcatgacgct gtacatgttc
     1321 acgaaggaca cccagggcgc ctcggccagt gcctgcgcag gggcctgcct cgcggcctgg
     1381 ccgcaggtcg tcgccggttc gaccgtgccc acgctgacgg gcgtctcggg cgccgtggcg
     1441 atgatcgcca ccgccgacgg gcgcagacag gtcactctgg ccggatggcc cctctactac
     1501 ttcgcgaagg acaaggcgcc cggcgacgtc ctcggccaag gcgtgaacca ggtctggtac
     1561 gtcctcgacc aagccggcac gcctgtcaag aagtagccac ggctgcgggc cgcactcgtc
     1621 ctggccggta gggtcgagac gcaggcgcgt cggcggttgc tgacgaccgg ggctggcgga
     1681 gggcacatga gtcgaggacc ggttcagggg tcgtccgggg tcacgctgac cgcgaccggg
     1741 gtgtccaagg agatctcggg cctaccgctg ctggccccgg tcgacctcat cgcccggccg
     1801 ggggagtgcg tcgtgctgcg gggcaagaac gggagcggca agacgaccct gctccgcatc
     1861 ctcgcgggca ccctggagcc gagtgcgggc cggctggaca tcgacgggca gcccgtcgac
     1921 gagcgcgcgc gtcatacccg ggagaccgtt gcggcgctgg tcggcgccat cgcgacctac
     1981 cgcgacctga ccctgtggga ccacctcgtc ctcgtcgagt cgacctgggg gctggacccg
     2041 gacacgaccg aggaccgggt ccaggccatg ctcgagctgc tcgagatcga ccacctcgac
     2101 gaccgcttcc cgcacgagct gtcctcgggg cagcagcagc tgttccatct ggcgctcgtg
     2161 ctcatccgcc cggcccggct gctcctgctc gacgagcccg aacagcggct cgacaccgac
     2221 aagcgccggc tgctcgcgca gatcctggct gcccgagcgg cgcagggcac gaccgtcgtc
     2281 atggcctgcc acgacccggt cgtcaccgag gccgtcgccg accgcatcct cgacctcgaa
     2341 cccgcctcgt gaccgccgac cgggcgcggg tcgtcgaggc ccgcgggatc atccacggcc
     2401 cggacgacac gagcgcccgc gacaaggccg ggtatgcgtt gtatgtcgcg gtcatcgtcg
     2461 cgctgtcgta tggcgtcccc gcgtcgcaac agat
//
LOCUS       DATAIX010000313         5260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_76726, whole genome shotgun sequence.
ACCESSION   DATAIX010000313 DATAIX010000000
VERSION     DATAIX010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76726"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1812)
                     /gene="treS"
                     /locus_tag="VHM65_04650"
     CDS             complement(97..1812)
                     /gene="treS"
                     /locus_tag="VHM65_04650"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEX2705026.1"
                     /translation="MSLPGLNVGQPGLRHDPDWFRTAVFYEVLVRAFADSKGSGAGDI
                     TGLIGKLDYLQWLGIDCLWLPPFYASPLRDGGYDIADYRSVLAEFGTLPEFTELVSQA
                     HARGIRIIVDLVMNHTSDQHPWFRASRSDPAGPYGDYYVWSDDDTKYSDARIIFLDTE
                     ESNWTFDPIRRQFFWHRFFSHEPDLNFENPKVQDEMFDVVRYWMNLGIDGFRLDAVPY
                     LFEEEGHNGENHPRTHEFLARLRAMVDAEFHGRILLAEANQMPADVVDYFGTPGAPEC
                     QMCFHFPVMPRIYYALRREKASAIISVLADTPPIPDGAQWGTFLRNHDELTLEMVPPE
                     QRAAMYGWYAPDPRMRANIGIRRRLSTLLDNSRAEFELINALLLCLPGSPCLYYGDEI
                     GMGDNIWLTDRDAVRTPMQWTPDRNAGFSAADPGKLHLPVVTSLVHHYSYINVEAQLA
                     CKSSRLHWVRGMLQVRSRHPVFGMGDFAIADADNEAVLAFVRAMDEVDADRPAQAVLC
                     VNNLASRPQATRIQLSDGYSGAHLVDLFGGTGFPDIGADGRLTLTMGSRDFFWFQLVA
                     ARQHD"
     gene            complement(1809..3830)
                     /locus_tag="VHM65_04655"
     CDS             complement(1809..3830)
                     /locus_tag="VHM65_04655"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEX2705027.1"
                     /translation="MTSSLPSGVAPIGRIPVIGVTPLVDGGTRPAKAVVDEEFVVTAT
                     VFREGHDAAKASVVLTAPDGTEHVQTMACDNPGLNHWRANVAPTSTGWWTYRVEGWSD
                     PYGTWEHDATIKVEAGVDVEVMLEEGARVLERALDNLGPAAPDPAVLRHAVTALRDVT
                     RPAMARLRAGTALPVRTYLAARPLREMVSPSPSYRLLVERERALYGAWYEFFPRSEGC
                     HRDKKTGRWVSGTLRTAGDRLPAIAAMGFDVVYITPIHPIGSAAKKGRNNSLIAGPDD
                     PGSPYAIGSPDGGHDAIHPDLGTFDDFDAFVARAAELGLEVAMDLALQCSPDHPWVVS
                     HPEWFTTRADGTIAFAENPPKKYQDIYPMNFNNDPAGVYAEVRRVVQVWIDHGVKIFR
                     VDNPHTKPVEFWQWLISDVAAQDPDVIWLAEAFTQPAMMHTLGKVGFQQSYAYFTWRN
                     LKWELEEYARELSGDAASYMRPSFWPTTHDILTPYLQYGGPIAWKLRAALAGTLVPTY
                     GIYSGYELMECVARPGVEEQIDNEKYQYKYRPWSAYEPGGAKHARSLAPYLTRINEIR
                     RAHPALHWLRNLTFHHVEDENLMCFSKSRWVGGADGYRDTVIVVANLDPHATRDSRVH
                     LDMAALQMSWHDQFVVQDLITDATWSWAEHNYVRLGPETEPVHILAVRS"
     gene            4116..>5260
                     /gene="glgP"
                     /locus_tag="VHM65_04660"
     CDS             4116..>5260
                     /gene="glgP"
                     /locus_tag="VHM65_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX2705028.1"
                     /translation="MKAIRRFSVRTVLPEPIAALGELASNLHFSWHPPTRALFASIDQ
                     RLWDRVNEDPVGLLSLLSARQLGALAEDGDFVARVNTASGALHHYLTEPRWYQEWAAT
                     VDGGAPRAIAYFSPEFGITHVLPQYSGGLGILAGDHLKSASDLGVPIVGVGLFYKTGY
                     FKQSLNNVGWQQETYPVLDPDGLPLALLREPDGTSCVVEINLPGSRVLKAHIWRAQVG
                     RVPLLLLDSDVPGNDDAARGVTESLYGGGGDRRLEQELLLGVGGVRALRVWSRLTGAP
                     DPDVYHTNEGHAGFQGVERINELMRDFDLTFDEALEGVRAATVFTTHTPVPAGIDRFG
                     ADKIHLYFGEHGAVPGVPLDRLLALGREDFEGGWRGVFNMAVMGLRLG"
BASE COUNT          773 a         1717 c         1916 g          854 t
ORIGIN      
        1 cgcagctgcg ggaggcgacc cttaccggca taccagcgct ggctcgagac ccagcccggc
       61 aaggagtcgg cgaacgacgg tgcaagggcg gcgtgctcag tcatgctgcc tcgccgccac
      121 gagctggaac cagaagaagt cacgcgagcc catcgtcagc gtgagccggc cgtccgcacc
      181 gatgtcgggg aacccagtgc caccgaagag gtcgacgagg tgggctccgg agtagccgtc
      241 ggacagctgg atcctcgtgg cctgcgggcg tgaggccagg ttgttgacgc agagcacggc
      301 ctgagccggg cggtcggcgt cgacctcgtc catcgcccgg acgaaggcca gcacggcctc
      361 gttgtcggcg tcggcgatgg cgaagtcgcc catcccgaag accgggtggc ggctgcggac
      421 ctggagcatc ccgcggaccc agtgcagacg cgaggacttg caggcgagct gggcctcgac
      481 gttgatgtag ctgtagtggt ggacgagtga ggtgacgacc gggagatgga gcttgcccgg
      541 gtcggccgcc gagaagcccg cgttgcggtc cggggtccac tgcatcggtg tgcggacggc
      601 gtcgcggtcg gtgagccaga tgttgtcgcc catgccgatc tcgtcgccgt agtagaggca
      661 tggtgagcca ggcagacaga gcaggagggc gttgatgagc tcgaactcgg cccgggagtt
      721 gtcgagcagg gtcgacaggc gccggcggat gccgatgttg gcacgcatgc gcgggtcggg
      781 ggcgtaccag ccgtacatcg ctgcccgctg ctccggcggg accatctcga gggtgagctc
      841 gtcgtggttg cgcaagaacg tgccccactg ggccccgtcc gggatcggcg gggtgtcggc
      901 caggaccgag atgatggcgg acgccttctc ccggcgcagc gcgtagtaga tccgcggcat
      961 gaccgggaag tggaagcaca tctggcactc gggcgccccc ggcgtgccga agtagtcgac
     1021 gacgtcggcg ggcatctggt tggcctcggc aagcaggatc cgcccatgga actcggcgtc
     1081 gaccatggcc cgcagccggg ccaggaactc gtgcgtcctg gggtggttct cgccgttgtg
     1141 tccctcctcc tcgaagaggt aggggaccgc gtccagccgg aagccgtcga tgccgaggtt
     1201 catccagtac cggacgacgt cgaacatctc gtcctggacc ttggggttct cgaagttgag
     1261 gtccggctcg tgcgagaaga agcggtgcca gaagaactgg cgccggatcg ggtcgaacgt
     1321 ccagttcgac tcctcggtgt cgaggaagat gatccgcgcg tcggagtact tggtgtcgtc
     1381 gtcggaccac acgtagtagt cgccgtaggg cccggcgggg tccgagcggc tggcccggaa
     1441 ccacgggtgc tggtcgctcg tgtggttcat gacgaggtcg acgatgatgc ggatgccgcg
     1501 cgcgtgcgcc tgcgagacga gctcggtgaa ctcgggcagg gtcccgaact cggcgagcac
     1561 cgagcggtag tcggcgatgt cgtagccgcc gtcacgcagg ggagaggcgt agaacggcgg
     1621 cagccagagg cagtcgatgc cgagccactg caggtagtcg agcttgccga tgaggccggt
     1681 gatgtcccct gcgcccgagc ccttggagtc ggcgaaggcc cgcacgagaa cctcgtagaa
     1741 cacggccgtc ctgaaccagt ccgggtcgtg ccgcagaccg ggctgaccga cgttgaggcc
     1801 cggcaggctc atgaccgtac ggccaggatg tgcaccggct ccgtctctgg gccgagacgc
     1861 acgtagttgt gctcggccca ggaccacgtc gcgtcggtga tgaggtcctg gacgacgaac
     1921 tggtcgtgcc agctcatctg cagcgccgcc atgtcgaggt ggacccgcga gtcccgggtc
     1981 gcgtgcgggt cgaggttcgc cacgacgatg accgtgtcgc ggtagccgtc ggcgccgccc
     2041 acccaccgtg acttcgagaa gcacatgagg ttctcgtcct cgacgtggtg gaacgtcagg
     2101 ttgcgcagcc agtgcagggc ggggtgggcc cggcggatct cgttgatcct cgtcaggtag
     2161 ggcgcgagcg accgcgcgtg cttggctccg cccggctcgt acgcggacca gggccggtac
     2221 ttgtactggt acttctcgtt gtcgatctgc tcttctacgc cggggcgcgc cacgcactcc
     2281 atcagctcgt acccgctgta gatgccatag gtcggcacga gcgtccccgc gagcgccgcc
     2341 cgcagcttcc acgcgatggg gccgccgtac tgcaggtagg gcgtgaggat gtcatgcgtc
     2401 gtcggccaga acgacgggcg catgtatgac gcggcgtcgc cggacagctc acgggcgtac
     2461 tcctcgagct cccacttgag gttgcgccag gtgaagtagg cgtagctctg ctggaagccg
     2521 accttgccca gcgtgtgcat catcgcgggc tgggtgaacg cctcggcgag ccagatgaca
     2581 tctggatcct gcgcggccac gtcactgatg agccactgcc agaactcgac cggcttggtg
     2641 tgcgggttgt cgacccggaa gatcttgaca ccgtggtcga tccacacctg gacgacccgg
     2701 cgaacctcgg cgtagacccc ggccgggtcg ttgttgaagt tcatcgggta gatgtcctgg
     2761 tacttcttcg gcgggttctc ggcgaaggcg atcgtgccgt cggcccgggt cgtgaaccac
     2821 tcggggtggg agaccaccca ggggtggtcc ggtgagcatt gcagggcgag gtccatggcg
     2881 acctccaggc ccagctccgc ggcgcgcgcg acgaaggcat cgaagtcgtc gaaggtcccg
     2941 aggtcggggt ggatcgcgtc gtgcccgccg tcgggcgagc cgatcgcgta cgggctaccg
     3001 gggtcgtccg ggcccgcgat gaggctgttg ttgcggccct tcttggcggc gctcccgatc
     3061 gggtggatcg gggtgatgta gacgacgtcg aagcccatgg ccgcgatcgc cggcaggcgg
     3121 tcccccgcgg tgcgcagcgt cccggacacc cagcggccgg tcttcttgtc ccgatggcac
     3181 ccttccgaac gcgggaagaa ctcgtaccac gcgccataca aggctcgctc gcgttcgacg
     3241 aggagccggt atgacgggct cgggctcacc atctcgcgca gcggacgagc ggcgaggtag
     3301 gtgcgcaccg ggagggcggt cccggcacgc aaccgggcca tcgcggggcg ggtgacatcg
     3361 cgcagggcgg tcacggcgtg gcgcaggacg gccgggtccg gggccgcggg gccgaggttg
     3421 tcgagggctc gttcgaggac ccgcgcgccc tcctcgagca tgacctcgac gtcgaccccc
     3481 gcctcgacct tgatcgtcgc gtcgtgctcc cacgtgccgt agggatccga ccaaccctcg
     3541 acgcggtagg tccaccagcc ggtcgaggtc ggggcgacgt tcgctcgcca gtggttgagg
     3601 ccggggttgt cacaggccat cgtctggacg tgctcggtgc cgtcgggggc agtgaggacg
     3661 acggaggcct tggcggcgtc gtgcccttca cggaacaccg ttgcggtgac gacgaactcc
     3721 tcgtcgacga cggccttggc cgggcgggtc cccccgtcga cgaggggagt gaccccgatg
     3781 accgggatgc gtccgatcgg ggccaccccg ctgggcagcg agctcgtcac ggagaagggc
     3841 tcgggcactg gctcggctgg ggtggcgcgc ggcgacctcg tcgacgagac cgcctcgttc
     3901 gtcgctgtcg gcctgtcctg cacggccggc ggcgcagcct tcttcgtgcg actggccgtg
     3961 ggtggagagg agggcgtggc gttcacagtc cgacgctacc ggctccgccc cgtttgtcac
     4021 atgtccagcc acgtcgtctg tcgtgtcgcg agtgttcagg cgatgactgg ggcaagttga
     4081 gttgagccgg accggacggc ctatagtggc ggctcgtgaa ggccatccga cgcttcagtg
     4141 tccgcaccgt gctgcccgag cccatcgccg ccctcggcga gctcgcgagc aacctgcact
     4201 tctcctggca tcctcccacc cgggcgctgt tcgcgagcat cgaccagcgg ctctgggacc
     4261 gggtcaacga ggacccggtc gggctcctgt cgctgctgtc cgcgcggcag ctcggggccc
     4321 tggccgagga cggcgacttc gtcgcccgcg tcaacacggc gagtggcgcg ctgcaccact
     4381 acctcaccga gccccggtgg taccaggagt gggccgccac ggtcgatggc ggtgcgccca
     4441 gggccatcgc ctacttcagc ccggagttcg gcatcacgca cgtgctgccg cagtactccg
     4501 ggggcctggg catcctcgcc ggtgaccatc tcaagtcggc ctccgacctt ggcgtgccga
     4561 tcgtcggcgt cggcctgttc tacaagaccg gctacttcaa gcagtcgctc aacaacgtgg
     4621 gctggcagca ggagacctac ccggtcctcg acccggacgg cctgcctctg gcgctgctgc
     4681 gcgagcccga cggcacgtcg tgcgtcgtcg agatcaacct gcctggctcg cgggtgctca
     4741 aggcccacat ctggagggcc caggtcgggc gggtcccgct gctcctgctc gactccgacg
     4801 tgccgggcaa cgacgatgcc gctcgcgggg tcaccgagtc cctgtatggc ggcggcggtg
     4861 accgacgtct ggagcaggag ctgctcctcg gcgtcggcgg tgtccgggcc cttcgggtct
     4921 ggtcccggct caccggtgct ccggacccgg acgtctacca caccaacgag ggccatgcgg
     4981 gcttccaggg tgtcgagcgg atcaacgagc tcatgcgcga cttcgacctc accttcgacg
     5041 aggcgctcga gggcgtccgc gctgcgacgg tgttcaccac ccacacgccg gtccccgcag
     5101 ggatcgaccg gttcggcgcc gacaagatcc acctctactt cggtgagcac ggcgccgtcc
     5161 cgggcgtccc gctcgatcgg ctgctggcgc tcggccgcga ggacttcgag ggcggctggc
     5221 gcggcgtctt caacatggcg gtcatggggc tgcgcctggg
//
LOCUS       DATAIX010000314         4034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_166303, whole genome shotgun sequence.
ACCESSION   DATAIX010000314 DATAIX010000000
VERSION     DATAIX010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166303"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VHM65_04665"
     CDS             complement(<1..1031)
                     /locus_tag="VHM65_04665"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEX2705029.1"
                     /translation="MSIISDAIETKKHGGALDEATIASVVGGYTAGSIPDYQMAALLM
                     AIRLKGMTYDETLALTTTMANSGVRYSFPDCVDKHSTGGVGDKVTLTALPLVAACGAP
                     VAKLSGRGLGITGGTIDKLESIPGWSADLSEHAFRAQVERIGLVVGGAGELAPADKVI
                     YALRDTTGTVDSLPLIGSSIVSKKAATGSGHLLYDVKAGSGAFMKTVEQARELAELLV
                     RLSASLGISSSALVTDMDTALGRAVGNALEVRESVAFLRGDPSAPDLAEVARAFAVRL
                     LELHGSSDPHGSVERAISSGAGYEKLREFVAAQGGDVDALTDLPVSPHRVEVLAPRDG
                     YVGAFDALG"
     gene            1072..1653
                     /locus_tag="VHM65_04670"
     CDS             1072..1653
                     /locus_tag="VHM65_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX2705030.1"
                     /translation="MLEPDADLDGETLTGSWVGARAERATLHECRVEDGDLTGLRAAH
                     SRWIGCTWTRVHGTDLDLSGSVWSEFTITDARLGAVALYGAQLRSGLVSGGKVDFLNL
                     RGAALADVSFEGAVLTEPDFAEARLTRVSFEGCRLVAPDFSRVVAKDLDLTGARIEAP
                     VGLASLRGATVSRLQLFDFAADLAAQLGIRVVG"
     gene            complement(1688..2101)
                     /gene="nusB"
                     /locus_tag="VHM65_04675"
     CDS             complement(1688..2101)
                     /gene="nusB"
                     /locus_tag="VHM65_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX2705031.1"
                     /translation="MAARSKARKRALDLLFEADQRGLNAATLLADRLASPGEGPALTQ
                     YTIILVQGVVAHWGDINELLSTYSHGWALERMPAVDRAVLRLGTFEILYCDDVPDPVA
                     IAEAVALARSLSTDESPSFVNGLLARIAEVKPTLV"
     gene            complement(2121..2684)
                     /gene="efp"
                     /locus_tag="VHM65_04680"
     CDS             complement(2121..2684)
                     /gene="efp"
                     /locus_tag="VHM65_04680"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX2705032.1"
                     /translation="MATTNDLKNGLVLSIEGQLWTVVEFQHVKPGKGPAFVRTKLKNV
                     MSGKVIDRTFNAGVRVETANVDKRTMAYLYKDGDDFVFMDGQTYDQIHVSSGTVGGAA
                     DYLLENQEAIVATHEGAPLFVELPTSVVLEISYTEPGLQGDRSTGGTKPATLETGAQI
                     AVPLFIETGTRVKVDTRDGSYLGRVND"
     gene            complement(2767..3648)
                     /locus_tag="VHM65_04685"
     CDS             complement(2767..3648)
                     /locus_tag="VHM65_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705033.1"
                     /translation="MSDDQHTKPTSTTPVSGVDMSLDEEDRGLLYDLPVLLERRRALM
                     LFGSAGLAALAACSASPSTGTPSASATGTSAADTSGASTSSALTEVPDETAGPYPGDD
                     SNGVNVLDDTGIVRSDITSSFGSTTAKAEGVPLTVRLTVKDVATSQALVGAAVYLWHA
                     DAQGRYSMYSQGVSAENYLRGVQSTEVSGTATFTSVYPGCYDGRWPHIHFEVYRSVEE
                     ATAAGQIVKTSQIALPQATCEQVYASNVYPDSAGNLAKVSLSTDNVFREDSGTSQLAT
                     MSGDTASGYTAARTIGV"
     gene            complement(3748..>4034)
                     /locus_tag="VHM65_04690"
     CDS             complement(3748..>4034)
                     /locus_tag="VHM65_04690"
                     /inference="COORDINATES: protein motif:HMM:NF037007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705034.1"
                     /translation="SEKQAGLNLPIITMIEGLLERGRSSGELHREVEAIELHLMMSAL
                     ALFRITNAPTIEATFGVRMNTPEYRARQIELLTSMVTTWLTTPDASDTGT"
BASE COUNT          560 a         1369 c         1440 g          665 t
ORIGIN      
        1 atcccgagcg cgtcgaacgc gccgacgtac ccgtcccggg gggcgaggac ctcgacgcga
       61 tgaggcgaca cgggcaggtc ggtgagcgcg tcgacgtccc caccttgagc cgcgacgaac
      121 tcgcgcagct tctcgtaccc ggcgcccgag gagatggccc gctcgaccga gccgtgcggg
      181 tcggagctcc cgtgcagctc cagcagccgg acggcgaagg cccgggccac ctcggcgaga
      241 tcgggtgcgc tcgggtcgcc cctgaggaag gccacgctct cgcgaacctc gagggcgttg
      301 cccacggctc ggccgagggc ggtgtccatg tcggtgacga gggccgatga gctgatcccg
      361 agcgacgcgc tgagccgcac gagcagctcg gcgagctcgc gggcctgctc gacggtcttc
      421 atgaaggcac ccgagccggc cttgacgtca tacaggagat gcccggagcc cgtggcggcc
      481 ttcttggaca cgatggagga gccgatgagc ggcagcgagt cgacggtgcc ggtggtgtcg
      541 cgcagggcgt agatgacctt gtcggccggg gcgagctcgc ccgctccccc gacgacgagc
      601 ccgatccgct cgacctgggc gcggaaggcg tgctcggaca ggtcggcact ccagccgggg
      661 atcgactcga gcttgtcgat cgtgccgccg gtgatgccga ggccacgccc ggacagcttg
      721 gccacgggcg ccccacacgc cgccaccagc gggagggcgg tcagagtcac cttgtcgccg
      781 acgccgccgg tcgagtgctt gtcgacgcag tccgggaagg agtagcgcac gcccgagttg
      841 gccatcgtcg tcgtgagggc gagggtctcg tcatacgtca tgcccttgag gcggatcgcc
      901 atgagcaggg ctgccatctg gtagtcgggg atcgacccgg cggtgtagcc gccgacgacg
      961 gaggcgatcg tcgcctcgtc cagcgcccca ccgtgcttct tggtctcgat cgcgtccgag
     1021 atgatgctca cccctcgatc gtgtcatggc cggggctggc acaatgtgcc cgtgctcgaa
     1081 cccgatgccg acctcgacgg cgagaccctg accggctcgt gggtcggcgc gcgcgccgaa
     1141 cgcgccaccc tgcacgaatg ccgggtcgag gatggcgacc tcaccgggct gcgcgcggcc
     1201 cactcgcgct ggatcggctg cacgtggacg cgcgtgcacg gtaccgacct cgacctgtcg
     1261 ggctcggtat ggtcggagtt cacgatcacc gacgcacgcc tcggcgctgt tgccctgtat
     1321 ggcgcgcagc tgcgctccgg cctcgtcagc ggcggcaagg tcgacttcct caacctgcgc
     1381 ggggcggcgc tggccgacgt gtcgttcgag ggcgccgtgc tgaccgagcc cgacttcgcc
     1441 gaggcccggc tgacccgggt gagcttcgag gggtgccggc tggtggcgcc cgacttctcg
     1501 cgggtcgtgg ccaaggacct ggacctcacc ggggcccgga tcgaggcacc ggtcggcctg
     1561 gcctcgctcc ggggcgcgac ggtgtctcgg ctgcagctgt tcgacttcgc cgccgacctg
     1621 gccgcccagc tcggcatccg cgtcgtcggc tgagcccgcc cccggctgcg gccggactca
     1681 gggcccgtca taccagcgtg ggtttgacct cggcgatccg ggccagcagc ccgttgacga
     1741 acgacgggga ctcgtccgta ctcagcgagc gggccagggc caccgcctcg gcaatggcga
     1801 ccgggtccgg cacgtcgtcg cagtagagga tctcgaaagt gccgaggcgc aggaccgccc
     1861 ggtcgaccgc tggcatccgc tccagggccc agccgtgcga gtaggtcgag agcagctcgt
     1921 tgatgtcccc ccagtgcgcg acgacgccct ggacgaggat gatggtgtac tgcgtgagtg
     1981 ccgggccctc tccgggcgaa gccagccggt cggcgagcag ggtcgccgcg ttgagcccgc
     2041 gctggtcggc ctcgaagagg aggtccagtg cgcgcttgcg ggctttgctc cgggcggcca
     2101 tgggggttct cgggtgcgcg tcagtcgttg acgcggccga ggtaggaccc gtcccgcgtg
     2161 tcgaccttga cccgggtgcc ggtctcgatg aacagcggga cggcgatctg ggcgccggtc
     2221 tcgagcgtcg ccggcttcgt gccaccggtg gagcggtcgc cctgcaggcc cggctcggtg
     2281 taggagatct cgagaaccac ggaggtgggc agctcgacaa agagcggggc cccctcgtgg
     2341 gtggcgacga tcgcctcctg gttctccagc aggtagtccg cagccccgcc gacggtcccg
     2401 ctcgagacgt ggatctggtc gtaggtctgc ccgtccatga agacgaagtc gtcgccgtcc
     2461 ttgtagaggt aggccatcgt gcgcttgtcg acgttggccg tctcgacccg cacccccgcg
     2521 ttgaaggtcc ggtcgatgac ctttccggac atgacgttct tgagcttggt ccggacgaac
     2581 gccggaccct taccgggctt gacgtgctgg aactccacga cggtccacag ctggccctcg
     2641 atggacagga cgaggccgtt cttgaggtcg ttggtcgttg ccaccggcta tcgcttcgct
     2701 ttcgtatgac gggcacgcgc aggcccgcgc acggacatct ggggatccga gagagtctac
     2761 ccgtgctcag accccgatcg tccgcgccgc cgtgtagccc gacgcggtgt ctccggacat
     2821 ggtcgcgagc tggctcgtgc cgctgtcctc gcggaagacg ttgtccgtcg acaggctgac
     2881 cttggccagg ttgcctgccg agtccgggta gacattcgat gcgtacacct gctcgcacgt
     2941 cgcctggggc aaggcgatct gggacgtctt gacgatctgg ccggccgcgg tggcctcctc
     3001 gacgcttcgg tagacctcga aatggatgtg cggccagcga ccgtcgtagc agccggggta
     3061 gaccgacgtg aacgtcgcgg tgccgctcac ttccgtgctc tggacacccc gcaggtagtt
     3121 ctcggcgctg accccttggg agtacatcga gtaccgcccc tgcgcgtcgg catgccagag
     3181 gtagaccgcc gccccgacga gcgcctggct cgtcgcgacg tccttgacgg tgagccgcac
     3241 cgtcagcggc acaccctcgg ccttggccgt cgtggacccg aagctcgagg tgatgtccga
     3301 gcgcacgatc ccggtgtcgt ccaggacgtt gacgccgttg gagtcgtcac ccgggtaggg
     3361 gcccgcggtt tcgtccggca cctccgtcag cgcgctcgac gtgctggctc ccgacgtatc
     3421 agcggccgag gtgcccgtgg ccgaagcgct cggggtgccg gtcgaaggcg acgccgagca
     3481 cgcggcgaga gcggccaggc cggcgctgcc gaagagcatc agcgcgcggc ggcgctcgag
     3541 cagcaccggc aggtcgtaga gcaggcctcg gtcctcctcg tccaggctca tgtccacccc
     3601 cgaaaccggc gtcgtggatg tgggcttggt gtgttggtcg tcactcatcg ggtctccctg
     3661 ggggggtggt cgcgtgtgcc aaggactgtc ctcggcgatg acgacgagcc tggcagtccc
     3721 gatcgtggct cccctttcga ccgactgtca ggttcctgtg tccgacgcgt cgggtgtggt
     3781 cagccaggtg gtcaccatcg aggtcagcag ctcgatctgg cgggccctgt actccggggt
     3841 gttcatccgg accccgaagg ttgcctcgat cgtcggcgcg ttggtgatcc ggaagagcgc
     3901 cagcgcactc atcatgagat ggagctcgat cgcctcgacc tcacggtgga gctcgccact
     3961 cgacctgccg cgctcgagca ggccctcgat catcgtgatg atcggcaggt tgagacctgc
     4021 ctgcttctcc gagc
//
LOCUS       DATAIX010000315         1821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_16836, whole genome shotgun sequence.
ACCESSION   DATAIX010000315 DATAIX010000000
VERSION     DATAIX010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16836"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1821)
                     /locus_tag="VHM65_04695"
     CDS             complement(<1..>1821)
                     /locus_tag="VHM65_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705035.1"
                     /translation="RRLGLGFDNRRTFATIDPGYYRWTQWIFLQIFSSWYDVTAVRAD
                     GGLGMARPITDLVGELESGSRPTPDGRAWASLSELERSRVVDGYRLAYTAEAPVNWCP
                     GLGTVLANEEVTNEGRSERGSYPVFRRSLRQWMMRITAYADRLADDLDRVQWPEKVKI
                     MQRNWIGRSSGARVTFPVRTVDGADEGIEVFTTRPDTLFGATFMVLAPEHPLVDHLVL
                     PGDWPEGTADIWKGAEAGAAVTPGAAVAAYRVAASRKSEVERQSEGKDKSGVFTGSYA
                     TNPLTGRPIPVFVADYVLMGYGTGAIMAVPAHDVRDYEFATHFALPIVDVVAPVGDSS
                     WAGTVAAGEAYVGDGAAVSSANAGVSLDGLGVADAKARIIGYLQASGIGAGTISYRLR
                     DWLFSRQRYWGEPFPIVFHADGTAHALPDSMLPLELPDVPDYSPKTFDPEDGASQPEP
                     PLSRASEWVNVDLDLADGRGVQRYRREANTMPNWAGSCWYYLRYLDPANSEALVDPAN
                     ERYWIGPHEGAVPGAPEGSRDPGGVDLYVGGVEHAVLHLLYARFWHKVLFDLGHVCSE
                     EPFRTYFSQGYIQAPAFTDARGQYVEAVEVLEHPGSHGAD"
BASE COUNT          277 a          655 c          633 g          256 t
ORIGIN      
        1 ggtcggcacc gtgcgacccg gggtgctcga gcacctccac ggcctcgacg tactgcccgc
       61 gcgcgtcggt gaaggccggc gcctggatgt agccctgcga gaagtaggtc cggaaaggct
      121 cctccgagca gacgtgcccg aggtcgaaga gcaccttgtg ccagaaccgg gcatagagca
      181 ggtgcagcac ggcgtgctcc accccaccga cgtagaggtc gacgccgccg gggtcgcggc
      241 tgccctcggg tgccccgggg acggcgccct cgtgagggcc gatccagtac cgctcgttgg
      301 ccgggtcgac gagggcctcg gagttcgccg ggtccaggta gcgcaggtag taccagcacg
      361 acccggccca gttgggcatc gtgttggcct cgcgcctgta gcgctggacg ccacgcccgt
      421 cggccaggtc gaggtcgacg ttgacccact cgctcgcccg ggacagcggc ggctccggct
      481 ggctcgcgcc atcctcgggg tcgaaggtct tgggcgagta gtcgggcacg tcgggcagct
      541 cgagcggcag catcgagtcg ggcagggcgt gggcagtccc gtcggcgtgg aagacgatcg
      601 ggaacggctc cccccagtag cgctgacggc tgaacagcca gtcacgcagc cggtagctga
      661 tcgtccccgc cccgatcccg ctggcctgca ggtagccgat gatccgcgcc ttggcgtcgg
      721 ccacgcccaa cccgtcgagt gacacgccgg cgttcgcgga gctgaccgca gcgccgtcac
      781 cgacataggc ctcgccagcg gcaaccgtcc cagcccagga cgagtccccc acgggggcaa
      841 cgacatcgac gatcggcagg gcaaagtgag tggcgaactc gtagtcgcgc acatcgtgcg
      901 cgggcacggc catgatcgcc ccggtgccgt agcccatgag cacgtagtcg gcgacgaaca
      961 ccgggatcgg tcgtcccgtc agggggttgg tggcatagga cccggtgaag accccggact
     1021 tgtccttgcc ctcgctctgg cgctcgacct ccgacttgcg tgaggccgcg acccggtatg
     1081 ccgccaccgc agctcccggg gtgaccgcgg cccccgcctc ggcgcccttc cagatgtcgg
     1141 ccgtgccctc gggccagtcc ccggggagca cgaggtgatc cacgagcggg tgctccggcg
     1201 ccaggaccat gaaggtcgcg ccgaacaagg tgtcaggccg cgtcgtgaag acctcgatcc
     1261 cctcgtcggc gccgtcgacc gtccgcaccg ggaaggtcac ccgcgcaccg ctggaccgcc
     1321 cgatccagtt gcgctgcatg atcttgacct tctccggcca ctggacccgg tcgaggtcgt
     1381 cggcgaggcg gtcggcatac gcggtgatcc gcatcatcca ctggcgcagg ctgcgccgga
     1441 agaccgggta gctgccccgc tccgagcgcc cctcgttggt gacctcctcg ttggccagca
     1501 cggtgccgag gcccgggcac cagttcactg gcgcctcagc ggtgtacgcc agccggtagc
     1561 catcgacgac tcgtgaccgc tcgagctcgg acaacgacgc ccaggcccgc ccgtccggcg
     1621 tcggacgcga ccccgactcg agctccccca ccagatcggt gatggggcgc gccatgccga
     1681 gcccgccgtc cgcgcggacg gctgtgacgt cataccagga gctgaagatc tgcaggaaga
     1741 tccactgggt ccagcggtag tagcccgggt cgatcgtcgc gaaggtgcgc cggttgtcga
     1801 accccagccc gaggcgccgc a
//
LOCUS       DATAIX010000316         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_186296, whole genome shotgun sequence.
ACCESSION   DATAIX010000316 DATAIX010000000
VERSION     DATAIX010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186296"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VHM65_04700"
     CDS             complement(<1..455)
                     /locus_tag="VHM65_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705036.1"
                     /translation="MPDDFLIARNPEEGSTLPFLIRLPLGRRGIVLKARETWPRTSKV
                     YCHRAEGWPQDAEVVERVPVRSCIQRGAAIDLVLHRGRENRSQLVFTRIRGGREAIFW
                     QTPRTAKQARPNVAIPTARASGLEDTLAVIVDSHERYAWTFTQQRVVTST"
     gene            complement(539..1840)
                     /locus_tag="VHM65_04705"
     CDS             complement(539..1840)
                     /locus_tag="VHM65_04705"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEX2705037.1"
                     /translation="MTSPVASTPQVPAVAAAGPFSAADASLAETVRGEASTVIAFLHR
                     YTGDFDIAEEAVQDAVVTALRTWREHGIPDRPGAWLTVAAKRRAIDLLRHRGRHLQAV
                     ETLAAQWPTEPEPTRHGDERVDMLFGCCHPSLAAEARLALTLRAVVGLTTAQIAQSFL
                     VPEATLAQRIVRAKRKIVSAGIPLRIPEPHLLADRLDDVLTVVYLTFNAGFLEPPTAG
                     PTAGPTAGPIAGPPDLSLASDAVWLANLVAARLPDEPEAWGLLALLTLQQSRAAARFD
                     ATGGLVLLADQDRSRWDHPDIARAEGYLEQAAVWRRPGRFQLQAAIAACHASAPTWAS
                     TDWLQILTLYDLLHRVDTSPVVALNRAVALAEYSGAHDGLAAVEPLGDRLAGYYLFHA
                     TRADLLRRLGRTDEALQANAQALALAANPVEQNLLRARLTR"
     gene            complement(1837..2202)
                     /locus_tag="VHM65_04710"
     CDS             complement(1837..2202)
                     /locus_tag="VHM65_04710"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX2705038.1"
                     /translation="MKHVLMYTNRPDLDAAIPAERQAEVYQAIFAWFEKHGAAFADMG
                     AELQGPATATTVKASQNGGDPVVVDGPFSEAKESIGGFSIIDVPDLDSALALAREWPS
                     LEFPGVAVEIRPVVEHPDM"
     gene            complement(2471..2614)
                     /locus_tag="VHM65_04715"
     CDS             complement(2471..2614)
                     /locus_tag="VHM65_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705039.1"
                     /translation="MTDRHTPDWPTRDWPTRDWPTPEQRRQLLGVIEGYYDSVPRSTA
                     PSS"
BASE COUNT          363 a          914 c         1067 g          375 t
ORIGIN      
        1 gtgctcgtca cgacccgctg ctgggtgaac gtccacgcgt agcgctcgtg gctgtcgacg
       61 atgacggcga gcgtgtcctc cagaccggac gcgcgggccg tcgggatggc gacgttgggg
      121 cgggcctgtt tggcggtgcg aggcgtctgc cagaagatcg cctcgcgccc gccgcggatg
      181 cgggtgaaca cgagctggga gcggttctcc cggccgcggt ggaggacgag gtcgatggcg
      241 gcgccgcgct ggatgcacga gcgcaccggc acccgctcga caacctcggc gtcctgtggc
      301 cagccctcgg cccggtgaca gtagaccttg ctcgtccgcg gccacgtctc gcgggccttg
      361 agcacgatcc cccggcgccc gagtggcagc cggatgagga agggcagcgt cgagccctcc
      421 tcggggttgc gggcgatgag gaagtcgtcc ggcacggccc cagtctggcg cctccgtctc
      481 gtccgcgtcg acacgatgtc gaggcgggcg cgcggcagcg cagcgaggcg gtggtcgcct
      541 accgggtgag ccgggcccgg agcaggttct gctcgaccgg gttggccgcg agggcgaggg
      601 cctgggcgtt cgcctgaagc gcctcgtcgg tccggccgag ccggcgcagc aggtcggccc
      661 gagtggcgtg gaagaggtag tagcccgcca gccggtcacc gagcggctcc accgcggcca
      721 gcccgtcgtg cgcgccggag tactccgcca gcgccacggc gcggttgagc gccacgaccg
      781 gtgaggtgtc gacccggtgc agcaggtcgt agagcgtgag gatctggagc cagtcggtgc
      841 tcgcccaggt cggtgcgctc gcgtggcagg cggcgatggc cgcctggagc tggaagcggc
      901 cggggcgtcg ccataccgcg gcctgctcga ggtagccctc cgcccgcgcg atatccgggt
      961 ggtcccaccg ggagcggtcc tggtcggcga gcaggacgag cccaccggtc gcgtcgaagc
     1021 gggcggcggc ccgggactgc tgcagcgtca ggagcgcgag cagcccccac gcctccggct
     1081 cgtccgggag ccgggccgcg acgaggttgg cgagccagac ggcgtcggag gcgagggaca
     1141 agtccggcgg cccggcaatg ggcccggcag tgggcccggc ggttggcccg gcggtgggcg
     1201 gctcgaggaa gccggcgttg aaggtgaggt agacgaccgt gaggacgtcg tcgaggcggt
     1261 cggcgagcag gtgcggctcg gggatgcgca gcggaatgcc ggcgctgacg atcttgcgtt
     1321 tggcccggac gatgcgctgc gcgagggtgg cctcgggcac gaggaacgac tgcgcgatct
     1381 gggcggtggt gagcccgacg accgcgcgca gggtcagggc aaggcgggcc tcggcggcca
     1441 gcgacgggtg gcagcagccg aagagcatgt cgacccgctc gtcaccgtga cgcgtgggtt
     1501 cgggctcggt cggccattgc gcggccaggg tctccacggc ctggaggtgc cgtccccggt
     1561 gccggagcag gtcgatggcc cgccgcttgg cggccacggt cagccaggcg cccggacgat
     1621 ccggtatgcc gtgctcacgc cacgtccgca gggcggtcac caccgcgtcc tgcacggcct
     1681 cttcggcgat gtcgaagtcg ccggtgtagc ggtgcaggaa cgcgatgaca gtgctggcct
     1741 caccccggac cgtctcggcc agggaggcat ccgcggccga gaagggcccg gcggcggcga
     1801 ccgcggggac ctgaggggtg ctggcgacgg ggctcgtcac atgtccgggt gttcgacgac
     1861 ggggcggatc tcgacggcaa cgccggggaa ctccagcgag ggccactcgc gcgccaacgc
     1921 cagggccgag tcgaggtcgg gcacatcgat gatgctgaac ccgccgatgc tctccttggc
     1981 ctcggagaag ggcccgtcga cgacgaccgg gtccccgccg ttctgggacg ccttcaccgt
     2041 cgtggccgtg gcggggccct gcagctcggc ccccatgtcg gcgaaggccg cgccgtgctt
     2101 ctcgaaccac gcgaagatcg cctggtagac ctcggcctgg cgctcggccg ggatggccgc
     2161 gtcgagatcg gggcggttgg tgtacatcag gacgtgcttc atcggtgtct ccttcggtcg
     2221 gggtccggcg tgaccggagt gctctggaca ccctgacgac gtctgggcag gcgtgatatc
     2281 gacaccatcg accagagcag ctcgctgcgc aagacctgca cggtggttcc gggcgagatc
     2341 tgggggccga tggtgtcccc gcggaaaccc actggccacc cgggccactg ggctggcacg
     2401 ctcgcagggc gaccgaccgc catactccgg actggccgac accggactgg ccgacaccag
     2461 cgcagcgggg tcagctgctc ggggccgtcg agcgggggac gctgtcgtag tacccctcga
     2521 tgaccccgag cagctgacgc cgctgctccg gtgtcggcca gtcccgtgtc ggccagtccc
     2581 gtgtcggcca gtccggggta tggcggtcgg tcaccctgcg agcgtgccag cccagtggcc
     2641 cgggtggcca gtgggtttcc gcggggacac catcggcccc cagatctcgc ccggaaccac
     2701 cgtgcaggtc ttgcgcagc
//
LOCUS       DATAIX010000317         2271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_16979, whole genome shotgun sequence.
ACCESSION   DATAIX010000317 DATAIX010000000
VERSION     DATAIX010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16979"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /locus_tag="VHM65_04720"
     CDS             <1..1484
                     /locus_tag="VHM65_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX2705040.1"
                     /translation="AGRRPSLIPLGYGLFMHDGEVVLGPRVDPAGDPLLVLRAALVAA
                     RNQLPLAAATVANLAELSPALPSPWPPEALAIFCDLLAAGPGLVQVWEGLDLAGVVEM
                     WIPQWAAVRSRPQRNLVHRHTVDRHLIETVVAAAGLVRHVARPDLLLLAALLHDIGKV
                     AGSLDHSDTGARIVVSILDRMGLSSDDAAVVVRLVREHLTLMDLATRRDPSDPKTVEA
                     VIEAAGGSADLLDQLAVLTEADAFAAGPLAWTDWRAQLFSRLVASARAHLRSGPDRVV
                     EEPTAELSVLTPEMTARLSMGQPCVTVSSHGGVHRIDIVDRDRHGLFADTAGLLAAQG
                     FVVRSALVRTIEGVAANEWHVESPGGDTPDPDGLARGLERLTGGDRTPLAPLSRRRRP
                     APIASARAGTGQPGQARALVLAHASDTATVIEVRATDRPGLLHDIGVTMARASLNVRS
                     AHISTYAGQALDTFYLSEFGSRPLSPARVAQAVSMIIDTCETV"
     gene            1649..>2271
                     /locus_tag="VHM65_04725"
     CDS             1649..>2271
                     /locus_tag="VHM65_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEX2705041.1"
                     /translation="MFNSLSDRLTATFKNLRGKGRLSESDVNKAVRDIRMALLDADVA
                     VPVVKGFTHAVRERALGAEVNGALNPAQQVVKIVNEELVAILGGQTRPLQLAKNPPTV
                     IMLAGLQGSGKTTFAGKLGRWLKDQGHTPILVAADLQRPNAVTQLEVVGDRAGVPVYA
                     PERGNVSGHDAVVPAGWGSSRGAGAGTRSYGDPVTVARAGVEHARAKL"
BASE COUNT          301 a          843 c          808 g          319 t
ORIGIN      
        1 gagcaggtcg tcggccatcg ctcatcccgc tcggctacgg gctgttcatg cacgacggcg
       61 aggtcgtgct cggcccgcgc gtcgacccgg cgggtgaccc gttactcgtg ctgcgggccg
      121 ctctggtcgc tgcccgcaac cagctcccgc tcgcggcggc caccgtggcc aacctggccg
      181 agctcagccc ggcgcttccc tcgccgtggc cgccggaggc actggccatc ttctgcgacc
      241 tcctcgcggc cggtcccggg ctcgtccagg tctgggaggg gctcgacctc gcgggggtcg
      301 tcgagatgtg gatcccgcag tgggctgccg tccgctcccg cccgcagcgc aacctcgtgc
      361 accgtcatac cgtcgaccgg cacctcatcg agacggtcgt cgccgccgcc gggctggtgc
      421 gccacgtggc ccggcccgac ctgcttctgc ttgctgcgct gctgcacgac atcggcaagg
      481 tcgccgggtc cctcgaccac agcgacaccg gagcccgcat cgtcgtttcg atcctcgacc
      541 ggatggggct gagcagcgac gacgcggcgg tggtcgtccg cctcgtccgc gagcacctca
      601 ccctgatgga cctcgcgacc cgccgcgacc cgtcggaccc caagacggtc gaggccgtca
      661 tcgaggcggc cgggggcagc gccgacctgc tcgaccagct cgccgtgctc accgaggccg
      721 atgcgttcgc ggccgggccc ctggcttgga ccgactggcg ggctcagctg ttcagccggc
      781 tcgtggccag cgcccgggcg cacctgcgct ccggcccgga cagggtcgtc gaggagccga
      841 cggccgagct gagcgtgctc acccccgaga tgacggcccg gctgtccatg gggcaaccgt
      901 gtgtcaccgt cagctcgcac gggggggtcc accggatcga catcgtcgac cgggaccggc
      961 acgggctgtt cgccgacacg gccgggctgc tggctgcgca gggtttcgtc gtacgctcgg
     1021 cgctcgtgcg gacgatcgag ggcgttgccg ccaacgaatg gcacgtcgag tcccccggtg
     1081 gagacactcc cgaccccgac gggctggccc gtgggctgga acgactgacc gggggggacc
     1141 gcaccccgct ggcgccgttg tcgcgccggc gtcgcccggc tccgattgcc agcgccaggg
     1201 cgggcaccgg ccagccgggc caggcgcgcg ccctggtgct cgcacacgcc tcggacacgg
     1261 cgacggtgat cgaggtccgg gcgaccgacc ggccgggact gctccacgac atcggcgtga
     1321 cgatggcccg ggcgagcctc aacgtccgca gcgcgcacat ctcgacctac gccgggcagg
     1381 cgctcgacac gttctacctc agcgagttcg gctcacggcc actctcaccc gcacgggtgg
     1441 cccaggcggt ctcgatgatc atcgacacgt gcgagacggt atgacggcgg acctgaccgg
     1501 ccgggctccc tgtcagagtg ccgacgtcgg gcaatgtccg ctcattccgg gtccatgacc
     1561 ggacgttgcc cgacgtcgcg gcgaggccgg gccgcggggt gcgcgcccgg gaccaggtgc
     1621 gcgccatccg ggcggtaagg ttctgcaggt gttcaactcc ctctccgacc ggctgaccgc
     1681 gaccttcaag aacctgcgcg gcaaggggcg gctgtccgag tccgatgtca acaaggcggt
     1741 ccgggatatc cggatggcgc tgctcgacgc cgacgtggcg gtgccggtcg tcaagggctt
     1801 cacccacgcg gtccgggagc gggcgctcgg cgccgaggtc aacggggcgc tcaacccggc
     1861 ccaacaggtc gtcaagatcg tcaacgagga gctcgtcgcg atcctcggcg gccagacccg
     1921 cccgctccag ctcgccaaga acccgccgac ggtcattatg ctcgcggggc tgcagggttc
     1981 gggcaagacg accttcgccg gcaagctggg ccgctggctc aaggatcagg gacacacacc
     2041 gatccttgtc gcggccgacc tgcagcgccc caacgcggtg acccaactcg aggtcgtcgg
     2101 cgatcgcgcg ggcgtgccgg tctacgctcc cgagcggggc aatgtcagcg gccacgacgc
     2161 cgtcgtgccg gccggctggg ggtcctcccg cggagcgggg gcgggaactc gctcgtatgg
     2221 cgacccggtg acggtggccc gcgcgggcgt cgagcacgcc cgcgccaagc t
//
LOCUS       DATAIX010000318         3473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_106830, whole genome shotgun sequence.
ACCESSION   DATAIX010000318 DATAIX010000000
VERSION     DATAIX010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106830"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VHM65_04730"
     CDS             <1..224
                     /locus_tag="VHM65_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705042.1"
                     /translation="IAVLAVAGVLVVAGCTGSGGGTPSTTSSTSAPAATSLTPTPSTT
                     WTTTVSMTPLQREAIAAVQAYYAAFNEGP"
     gene            991..1449
                     /locus_tag="VHM65_04735"
     CDS             991..1449
                     /locus_tag="VHM65_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705043.1"
                     /translation="MADVQAAARRAMEREVGRPRITVQPPGGRTLVNFTTIFSAPAQQ
                     VRSLPITVPVPGSVTGSPSYAWDLGEGITGQGSGSAYVEGMSPNDGSTDGLYVKARYR
                     QPGLKSVTATLTWEVDFELAGWGAVPLAPIVFTSAAATTAREARNVLVAP"
     gene            2209..>3473
                     /locus_tag="VHM65_04740"
     CDS             2209..>3473
                     /locus_tag="VHM65_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018529697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HEX2705044.1"
                     /translation="MSIQELFAGPMTDGRSDDRLPPQDVAAERSVLGGMLLSKDAIAD
                     CVEGLRGADFYRPAHEGIYDAILDLYGRGEPADPITVADELTKRGDLTRVGGQAYLHE
                     LVSSVPTAANAGYYAQIVAERAVLRRLVEAGTRIVQMGYAQNGDVEDIVNAAQAEVYG
                     VADKRGGDDYAILAELLEATMDEIEVASGKSGEMTGVPTGFTDLDTLTNGLHPGQMIV
                     IAARPAIGKALALDTPLVTPGGWTTMGEVQVGDLVVGADGRPAGVVAATEVMQGRPCF
                     EVEFSDGSVIVADAEHQWSVSVDSGVETVVTTRECAALRRAGATVAVERVPGRGSAYA
                     GAPAYAGASAYAGASAYPRAEVWKVTDVRAVPSVPVRCIEVDRPDHLYLAGRSMIPTH
                     NSTLGLDLARSASIKHRMASIVFSLEMSRT"
BASE COUNT          492 a         1045 c         1368 g          568 t
ORIGIN      
        1 ggattgctgt gctcgcggtg gcgggtgtgc tggtggtggc tgggtgtacg gggtccggcg
       61 gcggtacgcc gtcgacgacg tcgagcacgt cagcgccggc ggcgacgtcg ctgaccccga
      121 ccccgtcgac aacctggacg acgacagtgt cgatgacgcc gttgcagcgc gaggcgatcg
      181 ccgcggtgca ggcgtactac gccgccttca acgagggacc gtgaatcgtt cgtcggtcgc
      241 tttgaggggg ttgttcgcga gcgaatgcca gccgtgctcc cgctcggctt catcgatcga
      301 cggccttaag cgcgacaacc acacgatttc cggtggtgtc gttgctgtga agggactagg
      361 cagtctgcaa gggacggaag ctggcatcgg ggtccagggg acattgcagt caagcccggc
      421 gactctccgg gacagtcgtg gcgccacctt acacacctac aaggcgggta ctcaccccaa
      481 cactctgtgg ctcctgcgca gggagggccg tctcctcatg gtcgttgaga tcaggagcag
      541 ctaatgcacc gccgaacggc gatcctggtc gtctccctcc tgatgtccgc gggacttagc
      601 tgggtcgcgt cggactgggc acatgcggac tgctcggtgg tggtggacga gtggggcgcc
      661 gagaccacgg ggtacacgtg tccggggaag gtggggtgga gctacgactc gggtcgtagg
      721 cagttcctgc agggcgcgag gatccttggc cttgatgggc ctgtggaccc gcttgagtat
      781 cagaagtcgt cgagctgtgt gggtgatgcg cccggttcgg ctgggacgcc ggggtgtgcg
      841 caggcggcga gctgtccggt ggttctgggg acgaacggtc agccgctggg gtcgtggcgg
      901 gtcaacgcgt actcgcggct caggggctcg aacggcgcgt gggccctgat cgtgaggaac
      961 gcctgtgagt acaccagtga tgctgttccg ttggcggatg tgcaggcggc ggcgcggcgg
     1021 gcgatggagc gtgaggtggg ccggccgcgg atcacggtgc agccgcctgg gggtaggact
     1081 ctggtgaact tcacgacgat cttcagcgcc ccggcccagc aggtgaggtc gttgccgatc
     1141 acggttccgg tgccgggttc ggtgacgggg agcccgtcat acgcgtggga tcttggcgag
     1201 gggatcactg ggcagggttc ggggagtgcg tacgtggagg ggatgtcgcc caatgacggg
     1261 tcgacggatg ggttgtatgt gaaggcgcgc taccgccagc cggggctgaa gtcggtgacg
     1321 gcgacattga cctgggaggt cgacttcgag ctggcggggt ggggtgcggt gccgttggcg
     1381 ccgatcgtgt tcacctcggc ggcggccacg accgcgcgcg aggcgaggaa cgtgctggtc
     1441 gcgccgtagg ggtgtgtggg ggcccgcggc tgctcgtcgt ggaggtcgcg gctcggcctc
     1501 gccgacctag ccgaccaggt ggccatgggt gggtgctgcc ggggcggccg cgaccatcag
     1561 gtggccaccg ggcgtgcaac cgcggcggcc gcgaccatca ggtggccacc gggcgtgtac
     1621 ccacggcggc cgcgaccacc gggcgtgcac ctgcggcggc cgcgaccatc aggtggccat
     1681 cgggcgtgta cccacggcga ccgcgaccac cgggcgtgca cctgcggcgg ccgcgaccat
     1741 caggtggcca tcgggcgtgt acccgcggtg cgaaggtgcg cggcgccata ccgagagggt
     1801 atgtcgggac gctgaacgag cgctgtccaa ggcatggtgt cccggtggcc tggggatgag
     1861 actcgcccgg aagattcttt cgtccacagc cggtggagcc gcgttcgtgc acgtcgctag
     1921 gcgtggtggg cccccggggt cgcgcctgtc gtgcacacgt tgtccacagg cacgagggcg
     1981 ttcttccggc gtggcgcggt tcgtcatccc cacgttatcc acagggcatg gttgagcccg
     2041 ttgacgtggg ggcatagcgt gcgcaggtgg gtcgcccgac ggggctggcc cgggccgggc
     2101 agggtgtcgg tggtgggtga tccacttggt acatgagttc gactcgtccg gccggccagg
     2161 ctgggcgagg ccgtcgagtg tcgcctgagg gcgacggagg gggagcgggt gtcgatccag
     2221 gagctgttcg ccgggccgat gactgatggc cgatccgatg accgcctgcc gccgcaggat
     2281 gtcgcggccg agaggtcggt tctcgggggc atgctcctgt ccaaggacgc gatcgccgac
     2341 tgtgtcgagg ggctgcgtgg cgccgacttc taccgcccgg cgcacgaggg catctacgac
     2401 gccatcctcg acctgtatgg gcgtggggag cccgccgacc cgatcaccgt cgccgacgag
     2461 ctgaccaagc gcggcgacct gaccagggtc ggggggcagg cttacctgca cgagctcgtg
     2521 tcgtcggtgc cgaccgcggc caacgccggc tactacgcgc agatcgtcgc cgagcgggcc
     2581 gtgctgcgcc ggctcgtcga ggcggggacg cggatcgtcc agatgggcta cgcgcagaac
     2641 ggcgatgtcg aggacatcgt caacgccgcc caggcggagg tgtatggcgt cgccgacaag
     2701 cgcgggggcg acgactacgc gatcctggcc gagctgctcg aagcgacgat ggacgagatc
     2761 gaggtggcgt cgggcaagtc cggcgagatg accggcgtgc caacgggatt caccgacctc
     2821 gacacgctga ccaacggttt gcatccgggg cagatgatcg tcatcgccgc gaggcccgcc
     2881 atcggcaagg cgctcgctct cgacacgccg ctggtcaccc caggtggctg gacgacgatg
     2941 ggtgaggtcc aggtcgggga cctggtggtg ggtgccgacg gccgaccggc cggggtcgtg
     3001 gcggcgaccg aggtcatgca gggcaggccg tgcttcgagg tcgagttctc cgacgggtcg
     3061 gtgatcgtgg ccgatgccga gcaccagtgg tccgtcagcg tcgactccgg tgtcgagacg
     3121 gtcgtgacga cccgcgagtg tgcggcgcta cgccgggcgg gggccacggt tgcggtcgag
     3181 cgcgtgcccg ggcgcgggtc ggcatacgca ggggcgccgg catacgcagg ggcttcggca
     3241 tacgcagggg cttcggcata cccacgggct gaggtgtgga aggtgaccga cgtgcgggcg
     3301 gtgccgtcgg tgccggtgcg gtgtatcgag gtcgaccgcc ccgaccatct ctacctggcc
     3361 gggcggtcga tgatcccgac gcacaactcg accctggggc tcgacctggc gcggtcggcg
     3421 tcgatcaagc accggatggc ctcgatcgtc ttctcgctcg agatgagccg cac
//
LOCUS       DATAIX010000319         2379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_17058, whole genome shotgun sequence.
ACCESSION   DATAIX010000319 DATAIX010000000
VERSION     DATAIX010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17058"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VHM65_04745"
     CDS             complement(<1..487)
                     /locus_tag="VHM65_04745"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX2705045.1"
                     /translation="MRSLLLRARPVEVDLGGRVVTTWAYGDSLPGAAFRATAGERVQV
                     AFSNDLPEPTSVHWHGLAIRNDMDGVPGVTTPEVAAGGSFDFDFVVPDPGTHWFHPHH
                     GMQLDRGLYTPFIIDDPAEPGRYDAEWVVVLDDWTDGVGPSPEQIYASLKSGGGGSGG
                     MG"
     gene            862..1128
                     /locus_tag="VHM65_04750"
     CDS             862..1128
                     /locus_tag="VHM65_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705046.1"
                     /translation="MMGWYDNGLGLGMLLMLVVWGGLIGLGIWAVARLTRGESRPAPT
                     ESPRQILDRRFASGEIDAGQYAETRRILEGRGAVTPAPTPTPHG"
     gene            1177..1893
                     /locus_tag="VHM65_04755"
     CDS             1177..1893
                     /locus_tag="VHM65_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2705047.1"
                     /translation="MPDPLRVLVVDDEQALAGVVAGYLEREGFAVDLAYDGPAAVAAA
                     REHRPELVVLDVMLPGFDGIEVCRQLRGFSDAYIIMLTARDEEVDKVVGLSVGADDYL
                     VKPFSPRELIARVRAMLRRPRTVTGAAVDDAPAAGTQPLTVGGLVVDTVAHRASLDGG
                     PVDLTRTEFDLLATMAARPRAAFTRRQLIDAVWGPEWYGDEHVVDVHVGHVRRKLGDD
                     ASNPTHIRTVRGVGYGMATA"
     gene            1890..>2379
                     /locus_tag="VHM65_04760"
     CDS             1890..>2379
                     /locus_tag="VHM65_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX2705048.1"
                     /translation="MRRQPTGLAARLALAQLLVIATGALTLVVAAALVAPALFSEHLA
                     RTGEDSPVVRQHAEEAFASSFAISLALATVAALTASGLVSWFLARRVAHPVAELADAA
                     DAVAAGDYSARVPAGGFGTELIRLSHAFDHMSTRLAATEATRTSMLADLTHELRTPLA
                     TLE"
BASE COUNT          328 a          918 c          802 g          331 t
ORIGIN      
        1 tgcccatccc gccggacccg ccgccgccgg acttgaggct ggcgtagatc tgctcggggc
       61 tggggccgac gccgtcggtc cagtcgtcca gcacgacgac ccattcggcg tcgtagcggc
      121 cgggctcggc ggggtcgtcg atgatgaacg gcgtatacag gccgcggtcg agctgcatgc
      181 cgtggtgggg gtggaaccag tgcgtgcccg ggtcggggac gacgaagtcg aagtcgaacg
      241 accctccggc ggcgacctcc ggagtggtca ctccggggac gccgtccata tcgttgcgga
      301 tcgccaggcc gtgccagtgc acgctcgtcg gctcgggcag gtcgttgctg aacgccacct
      361 gcactcgctc gcccgcggtc gcccggaacg ccgccccggg cagagagtcc ccgtaggccc
      421 aggtcgtgac gacccggcca ccgagatcga cctcgacggg acgggcccgg agcaggagcg
      481 agcgcacccg gcccgtggag gcgcgggcgg actctgcagc cgcaaccgca tgtggcccga
      541 ccggagtagg tcccgaacgc acgccgcagg ccgacagccc tgctgcggcc cccgcaccgg
      601 ccagacccgc caagagctgg cggcgtgaca cggtcgtccc agcctgcatc gcgtctgccc
      661 cttcagcgtc tcggccgcca cgtgggcggg gcgcatccct cgcgccacga ccactatggg
      721 cgacgtcccc ggtgagttcc ccggaccttc gataaaggtt cgatgaagcg gacggcttca
      781 tcgaaccttc acgaccagcc acggctgatc gataccgcga cgtcgcacac tggaggtagt
      841 cgaagccgca ggaggcgcac catgatgggt tggtacgaca acgggctggg tctcggcatg
      901 cttctcatgc tcgtcgtctg gggcggcctg atcgggctgg gcatctgggc ggtcgcccgc
      961 ctcacccgcg gagagtcgcg gccagctccc accgagtccc cgcgccagat cctggaccgc
     1021 cgcttcgcca gcggcgagat cgacgccggg cagtacgccg agacccggcg catcctcgaa
     1081 ggccgcggcg cggtcacccc ggccccgacg ccgaccccgc acggctgacc aggactaccg
     1141 tcgtacgacg agacgcgacc cagccggagg atgcccatgc ctgacccgct tcgggtgctc
     1201 gtcgtcgatg acgagcaggc gctggcaggg gtggtcgccg gctacctgga acgcgaaggc
     1261 ttcgccgtcg acctcgcgta tgacgggccc gccgccgtcg cggccgcgcg ggagcatcgg
     1321 cccgagctgg tggtactcga cgtcatgctg ccggggttcg acgggatcga ggtgtgccgt
     1381 cagctgcgcg gcttcagcga cgcctacatc atcatgctta ccgcacgcga cgaggaggtc
     1441 gacaaggtcg tcgggctgtc ggtcggcgcg gatgactacc tggtcaagcc cttctcgccc
     1501 cgcgagctca tcgcccgcgt gcgcgcgatg ctgagacgcc cacgcaccgt caccggtgca
     1561 gcggtcgacg acgcgccggc ggcggggacc cagcccctga cggtcggtgg cctcgttgtc
     1621 gacaccgtcg ctcaccgcgc cagcctcgac ggtgggccgg tggacctcac ccgcaccgag
     1681 ttcgacctgc tcgccactat ggcggcccgc cctcgcgcag ccttcacccg ccgccagctc
     1741 atcgacgcgg tctggggccc ggagtggtat ggcgacgagc acgtcgtcga tgtccacgtc
     1801 ggacacgtgc gccgcaagct cggcgacgac gccagcaacc ccacccacat ccgcaccgtc
     1861 cgcggcgtcg gctacggcat ggcgaccgcg tgagacgcca gcccaccggc ctcgccgccc
     1921 ggttggcgct cgcgcagctg ctggtgatcg ccacgggcgc actcaccctg gtcgtggccg
     1981 ccgccctcgt cgcccccgca ctcttctccg agcacctcgc ccgcaccggc gaggactcgc
     2041 ccgtggtccg ccagcacgcc gaggaagcgt tcgcgtcctc cttcgccatc tcgctggcgc
     2101 tggcgaccgt ggccgccctc accgcgtccg gactggtgtc gtggttcttg gcccgccgcg
     2161 tcgcccaccc cgttgccgag ctcgccgacg ccgccgacgc cgtcgccgcc ggcgactact
     2221 cggcgcgcgt gccggccggt gggttcggca ccgagctcat ccgcctgtcg cacgcgttcg
     2281 accacatgtc cacccggctc gccgccaccg aggccacccg caccagcatg ctcgccgacc
     2341 tcacccacga gctgcgcacc cccctggcca ccctcgagg
//
LOCUS       DATAIX010000320         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_216026, whole genome shotgun sequence.
ACCESSION   DATAIX010000320 DATAIX010000000
VERSION     DATAIX010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216026"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1631)
                     /locus_tag="VHM65_04765"
     CDS             complement(96..1631)
                     /locus_tag="VHM65_04765"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004500.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX2705049.1"
                     /translation="MSTVPWLTIIGAIPLAGAIVVVLLPNRPSVRAKAVSLGFSLLTL
                     LVAILAATQFKTSAGTQFQLVENHPWIPQFGVSYALGVDGIALTLILMSVILTPVCIL
                     AAWHDVPDESAQRQQNYLALMLVLETFIVLVFSATDVFLFYVLFEGMLIPVYFLIGSY
                     GGARRQYASMKFLIFSLLGGLVMLVAVIALFVQGPGGPDGFLVERLTGLAMDPMTERL
                     LFVGFFFAFAVKAPMWPVHTWLPDAATEAPPATSVLLVGVLDKVGTYGMIRFCLQFFP
                     EASRWATPVVIVLAVISILYGALVAIGQTDMMRLIAYTSVSHFGFIVLGIFALTSTGG
                     VGATLYMLNHGFTTAALFLFVAMLVRRRGSKRIGDYGGWQRVTPNLAGVFLVAGLSGL
                     ALPGLGSFASEFLVLAGTFQRYQGAAVIGSLGIIMAALYILLMYKRTMTGPKPEGQEA
                     VFDLTTRDKLVAAPLIAVFLVLGFYPKPALDLITPAVRTTLAIVGVSDPPPSVEVTEG
                     STK"
     gene            complement(1631..>2096)
                     /locus_tag="VHM65_04770"
     CDS             complement(1631..>2096)
                     /locus_tag="VHM65_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705050.1"
                     /translation="LGAVLSIGGMFTRWLEPVVGAHPEGEPPVLAVPLITGLTLALIA
                     GGVVVAWVSYGRAVVPATAPAGSLLTRAARQDLYQDQVNEGLFMRPGIHLTRSLVFVD
                     NRGVDGAAGGLAAFVGGLSSRLRKVQNGYVRSYALTMLTGVVVILGAMWVIR"
BASE COUNT          399 a          662 c          783 g          252 t
ORIGIN      
        1 cccgaagacg atgagcatcg gtgcgactgc gacgtagtcg agcgtggcgg ccgtgaactc
       61 cgtgatggga cgacccgcgc cggccagctg ggcgatcact tggtgctccc ttcggtgacc
      121 tcgaccgaag gaggcgggtc gctgacgccg acgatggcga gtgtggtccg gactgcgggg
      181 gtgatcaggt ccagggccgg cttggggtag aagccgagga cgaggaacac ggcgatcagg
      241 ggggccgcca cgagcttgtc ccgggtcgtc aggtcgaaga ccgcctcctg tccctcgggt
      301 ttcgggccgg tcatcgtccg cttgtacatg agcaggatgt agagggcggc catgatgatg
      361 cccagcgacc cgatgaccgc cgcgccctgg tagcgctgga acgtccccgc gaggacgagg
      421 aactcagacg cgaacgagcc gaggcccggc agcgcgaggc ccgagaggcc ggccacgagg
      481 aacaccccgg cgaggttcgg ggtgacccgc tgccagccgc cgtagtcgcc gatccgcttg
      541 ctcccccggc gccgcacgag catggcgacg aagaggaaga gcgccgcggt cgtgaagccg
      601 tggttgagca tgtagagcgt cgccccgacc ccaccggtcg aggtgagcgc gaagatcccg
      661 aggacgatga agccgaagtg gctcaccgac gtgtaggcga tcaggcgcat catgtcggtc
      721 tgcccgatcg cgacgagcgc gccatagagg atcgagatca cggcgagcac gatgacgacg
      781 ggcgtggccc acctgctggc ctcggggaag aactgcaggc agaagcggat catcccgtag
      841 gtgccgacct tgtcgagcac gccgacgagc aggacggacg tcgccggggg ggcctcggtc
      901 gcggcgtcgg gcagccaggt gtggaccggc cacatcgggg ccttgaccgc gaacgcgaag
      961 aagaagccca cgaagagcag ccgctcggtc atcgggtcca tagccaggcc ggtgagccgc
     1021 tcgacgagga agccgtcggg gccgcccgga ccctggacga acagcgcgat gaccgcgacg
     1081 agcatgacga gaccgccgag cagcgagaag atcaggaact tcatcgaggc gtactgccgg
     1141 cgcgcgccgc catacgagcc gatgaggaag tagacgggga tgagcatgcc ctcgaagagc
     1201 acgtagaaga ggaacacatc cgtcgccgag aagaccagca cgatgaacgt ctcgaggacg
     1261 agcatgaggg cgaggtagtt ctgctggcgc tgggccgact cgtccgggac gtcatgccag
     1321 gcagcgagga tgcagaccgg cgtgaggatg accgacatga ggatgagcgt cagggcgatg
     1381 ccgtcgacgc ccagggcata gctgaccccg aactgcggga tccacggatg gttctcgacg
     1441 agctggaact gggtcccggc gctggtcttg aactgcgtcg cggccaggat cgccacgagc
     1501 agcgtgagca gcgagaagcc gagcgagacc gccttggccc gcaccgacgg gcggttcggg
     1561 aggagcacga cgacgatggc cccggcgagc gggatcgcgc cgatgatcgt gagccagggg
     1621 acggtggaca tcaccggatc acccacatgg cgccgaggat cacgacgacg ccggtgagca
     1681 tcgtcagtgc gtaggaccgg acgtagccgt tctggacctt gcgcagccgc gaggacaggc
     1741 ctccgacgaa ggcggcgagc cccccggcgg ccccgtcgac gccgcggttg tcgacgaaga
     1801 cgagcgagcg ggtcaggtgg ataccgggtc gcatgaacag cccctcgttg acctggtcct
     1861 ggtagaggtc ctggcgggca gcccgggtga gcaggctccc ggccggggcc gtcgccggga
     1921 ccaccgcacg cccgtagctg acccaggcga ccaccacgcc gccggcgatg agcgccaggg
     1981 tgagccccgt gatgagcggg accgcgagca cggggggctc gccctcaggg tgggccccca
     2041 cgacgggctc gagccagcgg gtgaacatcc ccccgatcga caggaccgca ccgagc
//
LOCUS       DATAIX010000321         3814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_245881, whole genome shotgun sequence.
ACCESSION   DATAIX010000321 DATAIX010000000
VERSION     DATAIX010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245881"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="VHM65_04775"
     CDS             complement(<1..1290)
                     /locus_tag="VHM65_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705051.1"
                     /translation="MADEQTPKTDAPTAAGAGGTAPGTGPASDKPTPAVPADDLVTTR
                     HTLKVGRRTLHYAATTGRVVLRDEIYEDGVFTGFKPKAEMSITAYVLEGAQVTSRPVT
                     FAFNGGPGSSSVWLHLGLLGPRRVLTGDVGALLPPPYGIADNPESLLAVSDLVFIDPV
                     STGYSRSVEGGKPGDYHGYAKDLESVGELIRLWTSRNERWMSPKFLAGESYGTLRAAA
                     LADHLQERYGMYLNGLMLVSSVLDLGSIGLHDADDRACVNFLPTYAAVAHYHGKHGRR
                     SLKSVLAEAEAYAERSYPWVLSRGARLTSAEVEEAATTIAALTGLSTDYVKRTNLRLE
                     HLAFFTELLRDEGKSVGRLDARFTGPAGSGTAEQYDADPSHDAVSGAYSAVFNHYVRS
                     ELGYANDLHYEQISNRVSPWSYKEFEGRAVDVTPRLAR"
     gene            complement(2155..3339)
                     /gene="galK"
                     /locus_tag="VHM65_04780"
     CDS             complement(2155..3339)
                     /gene="galK"
                     /locus_tag="VHM65_04780"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEX2705052.1"
                     /translation="MADTGIADSAAWEFEASFGGPPAGVWSAPGRVNLIGEHTDYNDG
                     LALPIALSQRTYAAVRLRPDRLLRLTSAQVGAMVEVSLDAVGPRHPGGWAAYLAGVFW
                     AMEQAGQAGGPLPGADIHVTSTVPWGAGLSSSAALECSVAAALSDLLGLGLLDSDAGR
                     AVLAEQCRRAENEIALAPTGGMDQAVAMRGLEGHALLLDCRDGSAEQVPVDLAGHGLA
                     LLVVDTRAEHALVDGQYASRRADCERSARELGVVALRDVEVAGLDGALTRLSSRVLRA
                     RTRHVVSEIDRVRRCVAALRASDFDEVGRLFLASHASLRDDFEVSCPELDAVVGAAMD
                     AGALGARMTGGGFGGSAIALVPAGLVAAVTDLVSSRFAHQGWRAPTPFEATAGPGACH
                     NA"
     gene            3633..>3814
                     /locus_tag="VHM65_04785"
     CDS             3633..>3814
                     /locus_tag="VHM65_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX2705053.1"
                     /translation="MQPTSDSLHLGNYLGALVNWVDLQEQFDTYYFIADLHALTVPTD
                     PAILRRRTRVTAAQFVA"
BASE COUNT          538 a         1335 c         1412 g          529 t
ORIGIN      
        1 ccgggcgagc cgcggggtca cgtcgacggc ccggccctcg aactccttgt acgaccacgg
       61 cgagacccga ttcgagatct gctcgtagtg aaggtcgttc gcgtacccga gctcgctgcg
      121 cacgtagtgg ttgaacaccg cggagtaggc cccgctcacg gcgtcgtggg aggggtccgc
      181 gtcgtactgc tcggccgttc cagagccagc cgggccggtg aaccgcgcgt cgagccgccc
      241 gacggacttg ccctcgtcgc gaaggagctc ggtgaagaag gcgaggtgct cgaggcgcag
      301 gttcgtccgc ttgacgtagt ccgtgctcag gccggtgagc gcggcgatcg tcgtcgcagc
      361 ctcctcgacc tcggcgctcg tgagccgggc gcctcgggag agcacccacg ggtagctgcg
      421 ctcggcatac gcttcggcct cggcgagaac gctcttgagg ctgcggcgtc cgtgcttgcc
      481 gtggtagtgg gcgacggcgg catacgtggg gaggaagttg acgcacgcgc ggtcgtccgc
      541 gtcatgcagc ccgatcgagc cgaggtcgag gacgctggag acgagcatga gcccgttgag
      601 gtacatgccg tagcgctcct gcaggtggtc ggcgagagcc gcggcgcgca gcgtcccgta
      661 ggactcgccg gcgaggaact tcggcgacat ccaccgctcg ttgcggctcg tccagagccg
      721 gatcagctcg ccgacgctct ccaggtcctt ggcgtagccg tggtagtcgc ccggcttccc
      781 gccctcgacg gaccgcgagt agccggtcga gacggggtcg atgaagacga ggtcgctcac
      841 ggcgagcagc gactcggggt tgtcggcgat gccgtaggga gggggcagca acgcgcccac
      901 gtcgcccgtg agaacacggc gcggcccgag cagcccgagg tggagccaca cgctcgagga
      961 cccggggccc ccgttgaagg cgaaggtgac cgggcgcgat gtgacctgcg ctccctcgag
     1021 cacataggcg gtgatcgaca tctcggcctt gggcttgaag ccggtgaaga ccccgtcctc
     1081 gtagatctcg tcgcgcagga cgactcgccc ggtcgttgcc gcgtagtgca gggttcggcg
     1141 acccaccttg agcgtatggc gggtggtcac caggtcgtcg gccgggaccg ccggcgtggg
     1201 tttgtcagat gctgggccgg tgcccggtgc cgtcccaccg gcgcccgcgg cggttggtgc
     1261 gtcggtcttg ggagtctgct cgtcagccac cccagcaccc tagcctgcgc ctggcgcccc
     1321 gggtggtggg caggcgcgcc accgcagcgg ggatactcgt gagtcgtgcg cgtcatcacc
     1381 gccaacgtca acggcatccg ggctgcggtg cgccgtgggg gccttcgctg gctggccggc
     1441 caggatgcgg atgtgctgct gctgcaggag gtccgggcta ccgacgagca ccttcacgct
     1501 gctctggccg aagccgggct cggcgactgg tcggtggcgc acaccgaggg caacgccagg
     1561 ggccgcgcgg gggttgccgt cgtgacccgg gcgcggcagc ggtcggtgcg cgtgggatgc
     1621 ggcggcctcg acgccggtga gttcggcgag agcggccgct gggtggaggc cgatgtcgac
     1681 accggccggg gttcggtgtc gttggcctcg gtgtatgtcc ccacggggga ggcggggact
     1741 gcgcgccagg ccgagaagga gcggttcctc gacgcgctga ccgctcgctt cgcagcagcc
     1801 cgtatggcgg gtgctcacct catcgtcggc ggtgacctca acgtggccca caccgacgcc
     1861 gacctgaaga actggaaggg caaccggggc aaggcagggt gcctgccggc cgagcgggcc
     1921 gtcctcgact cgtggcggga ccagcacgag atcgtcgacg tccaccgccg gcccgggccc
     1981 gggcccctat acgtggtgga cctggcgggg gaaggcgttc gacaccgaca cgggctggcg
     2041 catcgactac cagctcgcga cgaaggccgt tgccgagggc gctcgttcgg ccgtggtggg
     2101 gcgcgccgcg agctatgccg agcggtggag cgaccacgcg ccagtgacgg tcgactacgc
     2161 gttgtgacag gccccggggc ctgcggtggc ctcgaacggc gtcggagcgc gccacccctg
     2221 gtgtgcgaac cggctgctga ccaggtcagt cacggcggcg accagcccgg ccggcacgag
     2281 ggcaatggcg gacccgccga agccaccgcc ggtcatccgg gccccgagcg cgccggcgtc
     2341 catcgcggcc ccgacgacgg catcgagctc cggacaggac acctcgaagt cgtcgcgcag
     2401 ggacgcgtgc gaggcgagga agagccggcc gacctcgtcg aagtccgagg cccgcagcgc
     2461 ggcgacgcag cgccggaccc ggtcgatctc actgacgacg tgcctcgtgc gggcccgcag
     2521 aaccctggaa ctgagccggg tgagcgcccc gtcgagcccg gcaacctcga cgtcacgcag
     2581 cgcgacgaca ccgagctcgc gagccgagcg ctcgcagtcg gcccggcgcg aggcgtactg
     2641 accgtcgacg agggcatgct cggcccgggt gtccacgacg agcaacgcca ggccgtggcc
     2701 ggccaggtcg acggggacct gctcggccga gccgtcgcgg cagtcgagca ggagcgcgtg
     2761 cccctcgagg ccacgcatcg cgacggcctg gtccatcccg ccggtcgggg ccagcgcgat
     2821 ctcgttctcc gcccggcggc actgctcggc caggacggcc cgccccgcgt cggagtcgag
     2881 cagcccgagg ccgagcaggt cgctgagcgc cgcggcaacc gagcactcca gggccgccga
     2941 gctcgacagg ccagcgcccc acggcacggt gcttgtcacg tggatgtcgg cgccagggag
     3001 cggcccccca gcctgccccg cctgttccat cgcccagaag accccggcga ggtaggccgc
     3061 ccatcccccc ggatgcctgg ggccgaccgc gtcgagggac acctcgacca tggcgccgac
     3121 ctgggccgag gtgaggcgca ggaggcggtc cgggcgcagc cggaccgcgg cgtacgtccg
     3181 ctgggacagc gcgatcggca gggcgagccc gtcgttgtag tcggtgtgct cgccgatgag
     3241 gttgacccgc cccggggccg accagacccc ggccggcggt ccgccgaacg acgcctcgaa
     3301 ctcccacgcg gcgctgtcgg cgatgccggt atcagccatg gtgtgagggt cgcgagtgcg
     3361 agccgggcgc gtcaagtcgg gccgggtcct cgaccagcgt catcgtcgtc ctccagaccg
     3421 ggctctgcgc gttcgtcccg tccagggacc catgaggacg atccccaaaa agctctacac
     3481 gtcggccgtg tcgacggggc cagcgtcctg cgtcagctct tccggctcac gctggcgctg
     3541 ggctggcctg acccggaccg gaggggcccg gcatacctga cagaatggcc gttatgtctg
     3601 ccgcacctgc gcgtccccgc tctttgtcgg ggatgcagcc cacgtccgac tcgcttcacc
     3661 ttggcaacta cctcggggcg ctggtcaact gggtggatct gcaggagcag ttcgacacct
     3721 actacttcat cgccgacctg cacgcgctca cggtcccgac cgacccggcc atcctgcggc
     3781 gacgcacccg ggtcaccgcc gcccagttcg tcgc
//
LOCUS       DATAIX010000322         4843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_47427, whole genome shotgun sequence.
ACCESSION   DATAIX010000322 DATAIX010000000
VERSION     DATAIX010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47427"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..669
                     /locus_tag="VHM65_04790"
     CDS             118..669
                     /locus_tag="VHM65_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX2705054.1"
                     /translation="MTEDVSPETAQGFARVSDGFERLWTPHRMVYIEGAKPGSEAGES
                     CPFCAAPGRDDAAGLVVHRGTLCYVVMNLFPYNPGHILICPYRHVALFVDLTDEEAAE
                     VTALTRAGLRALDSASAPMGFNIGMNQGAVAGAGVTAHLHQHVVPRWVGDSNFLPIVA
                     HTKAMPMLLEDVRSRLVSAWPVG"
     gene            695..1303
                     /locus_tag="VHM65_04795"
     CDS             695..1303
                     /locus_tag="VHM65_04795"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX2705055.1"
                     /translation="MLNRLGRAAITRVLTPVARLLNALGISPDVVTIVGTLGVCFGAL
                     AFFPQNRLLVGTLVIVAFVFSDTVDGIMARMRGRSSTWGAYLDSTLDRVGDAAIFGGL
                     VLLYARQADLLMAGLALACLILGGVVSYAKARAEGLGMTANVGIAERADRLVAVLLAT
                     GLVGLGLPTAVLGVILGLLAIASAVTVVQRMLEVRRQALTSA"
     gene            1300..2259
                     /locus_tag="VHM65_04800"
     CDS             1300..2259
                     /locus_tag="VHM65_04800"
                     /inference="COORDINATES: protein motif:HMM:NF005919.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HEX2705056.1"
                     /translation="MSLVQGVKDAVVVAGYRVGWRVVRALPERPAYAMFDLIADLITR
                     RRGRGVRRLRANYAKVRPDLDDVALDRLVRDGMRSYLRYYCDAFRLPDRSVDELAAGV
                     HVSGDGPIRELLAAGTGVVVFIGHMGGWDTAGAWSTTQLAPVTTVVERLEPEAVFTTF
                     LGFRERLGMTILPLTGGPNPFTGMREALERGEFVALAADRDLTASGVEVDFCGHRARM
                     AKGPALLSAVTGAALFAAAITYQDAPEGEGIAGKMCHVDFSAPILPRVEGSTDAVVAD
                     LTQQCADYVGTAVQAYPSEWHMLQRVFVEDLDPARLARAVEPR"
     gene            2256..3443
                     /locus_tag="VHM65_04805"
     CDS             2256..3443
                     /locus_tag="VHM65_04805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2705057.1"
                     /translation="MRIGIVCPYSFDIPGGVQFHVRDLAEHFLGEGHHVSVLAPADDD
                     QPLPPYVVSCGRAVPVRYNGSVARLTFGPISAAKVGRWLDRGRFDVLHLHEPITPSAS
                     VLALWAAEGPTVATFHTANLRSRAMQAVFPLIRPSLEKIGGRIAVSEDARRTVTTHFG
                     GDAVVIPNGVYVDWFDSAAINPVWRGTRTRPTVAFLGRMEEPRKGMPVLAAALPEVLG
                     AIPGVRVLVAGPGDASAVTAGMAAKVAAACEFLGPVSDVDKARLLRSADVYVAPNTGG
                     ESFGIILIEAMSARAAVLASDLPAFVRVLDGGSAGATFSNEDPASLARELIGLLRDDA
                     RRAHLAVAGRARAELFDWSVVAEQVMAVYETVAAGAAVVRTTSEPGSRWARLLRTGRG
                     GDS"
     gene            3440..3988
                     /locus_tag="VHM65_04810"
     CDS             3440..3988
                     /locus_tag="VHM65_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705058.1"
                     /translation="MSTFEWVTLAVVLLVGLGWFLSYSAARLDRLHGRIEGTQAALDA
                     QLVRRAEATLELANTGTLDAASALILAGAATESLDSVDDLDPAREAVESELTEALEVA
                     LSTEVVELLRAEGGLGEEALERVRSAGLRVQLARRFHNDAVTDARRVRRKVSVRVFRL
                     AGHADMPRTFEFDDELPSAIPA"
     gene            4110..>4843
                     /gene="pdxS"
                     /locus_tag="VHM65_04815"
     CDS             4110..>4843
                     /gene="pdxS"
                     /locus_tag="VHM65_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX2705059.1"
                     /translation="MAEQHGALTTEAQHTVGTTRVKRGMAEMLKGGVIMDVVTAEQAT
                     IAEDAGAVAVMALERVPADIRAQGGVSRMSDPDMIDAIIAAVSIPVMAKARIGHFVEA
                     QVLQALGVDYVDESEVLTPADYANHIDKWAFTVPFVCGATNLGEALRRITEGAAMIRS
                     KGEAGTGDVSQATRHMREIRGQINRLRSMSSDELYVAAKELQAPYDLVADVARAGKLP
                     VVMFTAGGIATPADAAMMMQLGAEGV"
BASE COUNT          651 a         1710 c         1740 g          742 t
ORIGIN      
        1 atccggctca acgcgggcta tgggaagtcc gaggacagga ccggccgcgt catcccggtg
       61 gtcgttctcg accccgccgg accggctcga gtgggcccgc ggccctagga tcgacccgtg
      121 accgaggacg tcagccccga gaccgcgcag ggattcgccc gggtctcgga tggcttcgag
      181 cggctgtgga cgccgcatcg gatggtctac atcgagggtg cgaaacccgg ctcggaggcg
      241 ggggagtcct gtccgttctg cgccgctccc gggcgcgacg acgccgccgg cctcgtcgtg
      301 caccgcggca ccctgtgcta cgtcgtgatg aacctgttcc cctacaaccc cgggcacatc
      361 ctcatctgcc cctatcgtca cgtcgcactc ttcgtcgacc tcaccgacga ggaggcggcc
      421 gaggtcaccg ccttgacccg ggccggcctg cgggcactgg acagcgcctc ggcgccgatg
      481 gggttcaaca tcggcatgaa ccagggcgcg gtcgccgggg cgggcgtgac ggcgcacctg
      541 caccagcacg tcgtgccccg ctgggttggc gactcgaact tcctcccgat cgtcgcccac
      601 accaaggcga tgccgatgct cctggaggac gtgcgttccc ggctcgtgtc cgcctggcca
      661 gtaggctgac gaccatcatg acgagtcgac gacgatgctg aaccggctgg ggcgcgccgc
      721 catcacccgg gtcctcacgc ccgtcgcacg gctgctcaac gccctcggca tcagccccga
      781 cgtcgtcacg atcgtgggca cgctcggcgt gtgcttcggc gcgctggcct tcttccccca
      841 gaaccgcctg ctcgtcggga cgctggtcat cgtcgcgttc gtcttctccg acacggtcga
      901 cgggatcatg gcccggatgc ggggacgctc gagcacgtgg ggcgcctacc tcgactcgac
      961 tctcgaccgg gtcggcgatg cggcgatctt cggcgggctc gtcctgctct atgcccgcca
     1021 ggcggacctg ctcatggccg gcctcgcgct cgcctgcctc atcctgggcg gggtcgtcag
     1081 ctatgccaag gcccgggccg aagggctggg tatgacggcc aacgtcggga tcgccgagcg
     1141 ggccgaccgc ctcgtggcag tcctgctcgc caccggtctc gtcgggctcg gcctccccac
     1201 agccgtcctc ggtgtcatcc tcggactgct cgccatcgcc agcgcggtca ccgtcgtgca
     1261 gcggatgctc gaggtccggc gccaggcgct cacctccgca tgagcctcgt ccagggcgtt
     1321 aaggacgccg tcgtcgtggc cggctaccgc gtcgggtggc gggtcgtccg cgcgctgccg
     1381 gagcgcccgg catacgcgat gttcgacctc attgccgacc tcatcacccg ccgtcgcggg
     1441 cggggggtcc gccggctgcg cgccaactac gccaaggtgc ggccggacct cgacgacgtg
     1501 gcgctggacc ggctcgttcg cgacgggatg cggtcctatc tgcgctacta ctgcgacgcg
     1561 ttccggctgc ccgaccgctc cgtcgacgag ctcgccgcag gggtgcatgt cagtggtgac
     1621 gggccgatcc gtgagctgct cgcggcaggg acgggtgtcg tcgtcttcat cggccacatg
     1681 ggcggctggg acaccgccgg ggcctggtcg acgacccagc tggcgccggt caccacggtg
     1741 gtcgagcgcc tcgagccgga ggcggtgttc acgacgttcc tcggcttccg ggagcgcctg
     1801 ggcatgacga tcctgccgct caccgggggc ccgaacccgt tcaccgggat gcgggaggcg
     1861 ctcgagcggg gagagttcgt cgccctcgct gccgaccgcg acctcaccgc cagcggcgtc
     1921 gaggtcgact tctgcgggca ccgggcgcgg atggccaagg gcccggcgtt gttgtcggcc
     1981 gtcactggtg cggcgctctt cgccgcggcc atcacctatc aggacgcccc ggagggcgag
     2041 gggatcgccg gcaagatgtg ccacgtcgac ttcagtgcgc ccatcctgcc ccgcgtcgag
     2101 ggcagcacgg acgcggtcgt tgccgatctc acccagcagt gcgccgacta cgtcggcacc
     2161 gccgtgcagg cgtaccccag cgaatggcac atgctgcaaa gggtcttcgt cgaggacctc
     2221 gacccggccc ggctcgcccg ggcggtggag cccaggtgag gatcggcatc gtctgcccgt
     2281 actccttcga catcccggga ggagtgcagt tccacgtgcg ggacctcgcc gagcacttcc
     2341 tgggcgaggg ccaccacgtc tccgtgctcg ccccggccga cgacgaccag ccgttgccgc
     2401 cctacgtggt ctcctgcggc cgggccgtgc cggtgcgcta caacggctcg gtggcgcggc
     2461 tcacgttcgg gccgatctcg gcggccaagg tcgggcgctg gctcgaccgt ggcaggttcg
     2521 acgtcctgca cctgcacgag ccgatcaccc cgagcgcctc ggtccttgcc ctgtgggccg
     2581 ctgagggccc gaccgtggcc acgttccaca cggccaacct gcgctcgcgc gccatgcagg
     2641 cggtattccc gctcatccgc ccgagcctgg agaagatcgg cgggcgtatc gccgtctccg
     2701 aggacgcccg ccgcacggtg acgacccact tcggtggcga cgcggtcgtc atacccaatg
     2761 gggtgtatgt cgactggttc gactccgctg ccatcaaccc ggtatggcgg ggcacgcgaa
     2821 cgcgcccgac cgtggccttt ctcgggcgga tggaggagcc gcgcaagggg atgcccgtgc
     2881 tggcggcggc cctgccggag gtcctgggcg cgatcccggg cgtgcgggtc cttgtcgccg
     2941 ggcccgggga tgcgtcggcc gtgaccgcgg gtatggcggc gaaggtcgcc gccgcgtgcg
     3001 agttcctcgg accggtgagc gatgtggaca aggcgcggct cctgcggtcg gccgatgtct
     3061 acgtcgcgcc caacaccggg ggcgagagct tcgggatcat cctcatcgag gccatgagtg
     3121 cgcgggcggc ggtcctggcg agtgacctgc cggcgttcgt gcgagtcctc gacggcggct
     3181 cggcaggggc gaccttcagc aacgaggacc cggcgtcgct ggcgcgcgag ctcatcgggc
     3241 tgttgcgcga tgacgcgcgg cgggcccacc tggccgtggc cggccgggcg cgggccgagc
     3301 tctttgactg gtcggtcgtc gccgagcagg tcatggccgt ctacgagacg gtggccgcgg
     3361 gggccgcagt cgtccgcacg acgagcgagc ccgggtccag gtgggcccgg ctgttgcgga
     3421 ccggccgcgg aggggactcg tgagcacgtt cgagtgggtc acgctggcgg tggtcctcct
     3481 cgtcggtctg gggtggttcc tgtcgtactc cgccgctcgc ctcgaccgcc tccacggccg
     3541 catcgagggg acccaggcgg ccctcgacgc ccagctcgtc cggcgcgcag aggcgacgct
     3601 ggagctggcc aacaccggca cgctcgacgc ggcgagcgcg ctgatcctcg ccggtgccgc
     3661 gaccgagtcg ctggactccg tcgacgacct cgaccctgcg cgcgaggcgg tcgagagcga
     3721 gctgaccgag gccctggagg tggccctgtc gaccgaggtc gtcgagctgc tccgggccga
     3781 gggcggcctt ggcgaggaag ccctcgaacg agtccggtca gcagggctcc gggtccagct
     3841 ggcccgccgg ttccacaacg acgcagtcac cgacgcgcgc cgggtgcgcc gcaaggtcag
     3901 cgtgcgggtc ttccgcttgg cggggcatgc ggacatgcca cggaccttcg agttcgacga
     3961 cgagctgccc agcgccatac ccgcctgacc tggctcggcc atcgcgtgct cgacagcgcc
     4021 gtgctcggtc gtgcgtggtg aagcgagata ggctggagtg ctgaggcagg cgtgacatga
     4081 cgcagcccac gagagcgaga gcaaggacca tggcagagca gcacggcgcc ctgacgaccg
     4141 aggcgcagca cactgttggg acgacccgcg tcaagcgggg gatggccgag atgctcaagg
     4201 gcggcgtcat catggacgtg gtcaccgccg agcaggccac gatcgccgag gacgccggtg
     4261 cggtggccgt catggccctc gagcgggtgc ccgcggacat ccgcgcgcaa ggtggcgtgt
     4321 cgcgcatgag cgaccccgac atgatcgacg ccatcatcgc ggcggtctcg atccccgtca
     4381 tggccaaggc ccggatcggc cacttcgtcg aggcccaggt cctgcaggcg ctcggagtgg
     4441 actatgtcga cgagtccgag gtcctcaccc ccgctgacta cgccaaccac atcgacaagt
     4501 gggccttcac ggtgccgttc gtgtgcggtg cgaccaacct cggtgaggca ctgcggcgga
     4561 tcaccgaggg ggcggcgatg atccgttcca agggcgaggc cgggacaggc gacgtgtccc
     4621 aggcgacgag gcacatgcgc gagatccgtg gccagatcaa ccggctgcgc tcgatgtcga
     4681 gcgacgagct gtatgtcgcc gccaaggagc tgcaggcgcc ctatgacctc gtcgccgacg
     4741 tggcccgggc cggcaagctg cccgtcgtga tgttcaccgc gggtggtatc gcgaccccgg
     4801 ccgacgcggc catgatgatg cagctcggtg ccgaaggcgt ctt
//
LOCUS       DATAIX010000323         2532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_126735, whole genome shotgun sequence.
ACCESSION   DATAIX010000323 DATAIX010000000
VERSION     DATAIX010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126735"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /gene="gabT"
                     /locus_tag="VHM65_04820"
     CDS             <1..1206
                     /gene="gabT"
                     /locus_tag="VHM65_04820"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEX2705060.1"
                     /translation="AGGGILVDVDGNHLIDFGSGIAVTTVGNSAPRVVAAVQSQVEDF
                     THTCFMVTPYEEYVAVCEALAAVTPGSFAKRSALFNSGAEAVENAVKVARHYSGRNAI
                     VAFNHAYHGRTNLTMALTSKVMPYKHRFGPFAGDTYRAPFAYPYRWPGGREKCMEQAF
                     VEFEGFMEAQVGPKNTAAVIVEPLLGEGGFIVPAPGFLAAVQDYCRSHDILFIADEVQ
                     TGFCRTGDWFASEYEGLEPDLITTAKGMAGGLPLAGVTGRAEIMDAIHPGGLGGTYGG
                     NPTACAAALATIQMMRDEDLCGKARAIEALFLPRLRALQERYPLIGDVRGRGAMLAIE
                     LVMGADDMSPNPALATAVTRYCHSQGLVTLTCGTYGNVFRFLPALVTSHDLLTEGLGI
                     LDDAFAACA"
     gene            complement(1226..1906)
                     /locus_tag="VHM65_04825"
     CDS             complement(1226..1906)
                     /locus_tag="VHM65_04825"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF003588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX2705061.1"
                     /translation="MTTRPLRDLVHPSWVPALEPAEATLAALGEFLRTEVASGRGYLP
                     AGPHVLRAFSLPLDAVRVLIVGQDPYPSPGHAVGLSFSVGADVRPIPRSLANIYAELT
                     SDLGVPMPRCGDLTPWADQGVLLLNRVLTVRPGAPASHRGKGWEELTARALDALVARG
                     GPLVAILWGRHARDLAPGLGEVPRIESAHPSPLSARTGFFGSRPFSRANALLAQQGAP
                     PVDWSLPA"
     gene            1973..>2532
                     /locus_tag="VHM65_04830"
     CDS             1973..>2532
                     /locus_tag="VHM65_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX2705062.1"
                     /translation="MVTPRWPVAVFDLDGTLVDTIHLIVESYQHTFRTHRGHTEDEAR
                     IRSWIGRPLIEAFREVDAHEAEAMYATYLTWNRANTERLIRRYDGVEDLLVALRSAGV
                     RVAVVTSKRRDAALPALRLCALESYVDLLVTMENTTRHKPDPEPLRFALSQLGIGADQ
                     ACYVGDAAVDLLAAAAAGMAGIGVTWG"
BASE COUNT          373 a          934 c          883 g          342 t
ORIGIN      
        1 gccggcggcg gcatcctcgt cgacgtcgac ggcaaccacc tgatcgactt cggctccgga
       61 atcgcggtca ccaccgtggg caactcggcg ccacgagtgg tcgcggccgt gcagtcgcag
      121 gtcgaggact tcacccacac ctgcttcatg gtgacgccgt acgaggagta cgtcgccgtc
      181 tgcgaagcgc tggccgcagt gacgcccggc tcgttcgcga agcgctcggc gctgttcaac
      241 tccggcgcgg aggcggtcga gaacgccgtc aaggtcgcgc gccactacag cgggcgcaac
      301 gccatcgtcg ccttcaacca cgcctaccac ggccgcacca acctcaccat ggcgctgacc
      361 tcgaaggtca tgccctacaa gcaccgcttc ggcccgttcg ccggcgacac ctaccgcgcg
      421 cctttcgcct acccctaccg ctggccgggt ggccgggaga agtgcatgga gcaggcgttc
      481 gtcgagttcg agggcttcat ggaggcccag gtcggcccga agaacaccgc cgccgtcatc
      541 gtcgagccgc tcctcggcga gggcgggttc atcgtgccgg cgccgggctt cctcgccgcg
      601 gtccaggact actgccgcag ccacgacatc ctgttcatcg ccgacgaggt ccagaccggc
      661 ttctgccgca ccggcgactg gttcgcctcg gagtacgaag ggctcgagcc cgacctcatc
      721 accacggcca agggtatggc gggcggcctg ccgctcgccg gggtcaccgg ccgcgccgag
      781 atcatggacg cgatccaccc cggtgggctc ggtggcacgt atggcggcaa ccccaccgcg
      841 tgcgcggcgg cgctcgcgac gatccagatg atgcgcgacg aggacctgtg cggcaaggcc
      901 cgggccatcg aggcgctgtt cctgccgcgg ctgcgtgcgc tccaggagcg ctaccccctg
      961 atcggcgacg tccgcggccg tggggccatg ctcgcgatcg agctcgtcat gggcgccgac
     1021 gacatgtcgc cgaacccggc gctcgcgacg gcggtcacca ggtactgcca cagccagggc
     1081 ctggtgacac tgacctgcgg cacctacggc aacgtgttcc gcttcctgcc ggccctggtg
     1141 acgagccacg acctgctcac cgagggcctc ggcatcctcg acgacgcctt cgccgcctgc
     1201 gcgtaggacc ggcgagccgg cgggctcagg ccggcagcga ccagtcgacg ggcggtgcgc
     1261 cctgctgcgc cagcagtgcg ttggcccggc tgaagggccg cgacccgaag aagcccgtgc
     1321 gggccgacag cggtgaggga tgggcgcttt cgatgcgggg gacctcgccc agccctggcg
     1381 ccaggtcgcg ggcatggcgt ccccagagga tcgcgacgag cgggccgccc cgggcgacga
     1441 gggcgtcgag cgcccgtgcg gtgagctcct cccagccctt gccgcggtgg ctcgcgggcg
     1501 ccccgggccg cacggtgagc acccggttga gcagcagcac gccctggtcg gcccatgggg
     1561 tgaggtcgcc gcatcggggc atcggcaccc cgaggtcgct cgtcagctcg gcgtagatgt
     1621 tggccaggct gcgcgggatc ggccgcacgt cggcaccgac cgagaacgac agccccacgg
     1681 catggccagg gctcgggtag gggtcctgcc cgacgatgag gacccgcacc gcgtcgagcg
     1741 ggagggagaa ggcgcgcagg acgtgcggcc cggccggcag gtacccccgc cccgaggcca
     1801 cctcggtccg caggaactcc ccgagcgcag ccagcgtcgc ctcggccggt tccagagccg
     1861 gcacccacga ggggtgcacg aggtcacgta aggggcgggt cgtcataccg ccaaacctac
     1921 ccggcgtgga cgcagccccg gccccctcga acgctgacct aaggtgacgg ccatggtgac
     1981 cccccgctgg ccggtggccg tgttcgacct cgacggcacc ctcgtcgaca cgatccatct
     2041 catcgtggag tcctaccagc acaccttccg tacccaccgt ggccataccg aggacgaggc
     2101 ccgcatccgc agctggatcg ggcgcccgct catcgaggcc ttccgtgagg tcgacgcgca
     2161 cgaggccgag gcgatgtacg cgacctacct gacctggaac cgcgccaaca ccgaacgcct
     2221 gatccgccgg tatgacgggg tcgaggacct gctcgtggca ctgaggtccg cgggggtccg
     2281 ggttgccgtg gtcacctcca agcggcgcga cgctgccctc ccggcgctgc ggctctgcgc
     2341 gctggagtcc tatgtcgacc tcctcgtcac catggagaac acgacccggc acaagcccga
     2401 tccagagcca ctgcgcttcg ccctgtccca gctggggatc ggcgcggacc aggcctgcta
     2461 cgtcggggac gccgcggtcg acctgctcgc ggcggccgcg gccgggatgg cggggatcgg
     2521 ggtcacctgg gg
//
LOCUS       DATAIX010000324         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_47490, whole genome shotgun sequence.
ACCESSION   DATAIX010000324 DATAIX010000000
VERSION     DATAIX010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47490"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VHM65_04835"
     CDS             complement(<1..184)
                     /locus_tag="VHM65_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705063.1"
                     /translation="MTGRPTPSRRPSLRVERTLQRAGHRVLAGMDEVGRGALAGPVSV
                     GVVLIDEAVRSATLGVK"
     gene            complement(181..963)
                     /gene="lepB"
                     /locus_tag="VHM65_04840"
     CDS             complement(181..963)
                     /gene="lepB"
                     /locus_tag="VHM65_04840"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX2705064.1"
                     /translation="MADQTNGAASAASTQVRRGRHGGAPAPMSVGQRIVATVREVVLV
                     VIMAALISFVIKTWLVQAFYIPSESMEDTLVKNDRVIVSKLTPGPFDLERGDVVVFED
                     PKVPSPWLGQSVAGEPSTTQRVLTFIGLLPDDAKNHLIKRVIGLPGDHVQADGTGSIK
                     VNGVALTEFYVKAGDDPSTRPFDITVPAGRIWVMGDHRSDSSDSRFHDDGTGRTGSVP
                     IDVVTGRAIALVWPLGRMTWLGHQDDVFAKVPNAGLTTTPGS"
     gene            complement(998..>1518)
                     /gene="lepB"
                     /locus_tag="VHM65_04845"
     CDS             complement(998..>1518)
                     /gene="lepB"
                     /locus_tag="VHM65_04845"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX2705065.1"
                     /translation="ALAVTVLVLALVRAFVVNPFVLTSTSMQPSFQPGDRVLVARWQH
                     GGPLRRGDVVVFDGAAAFGGGTDGDYVKRVVAVGGDRVRCCTQEGRLSVNGVAVAEPY
                     LFPGEPASASPFDILVPAGRVFVLGDHRGESEDSRAHLGGPGGGTVAATDVIGQVWVR
                     YWPLDRLGGPGG"
BASE COUNT          235 a          549 c          529 g          205 t
ORIGIN      
        1 ccttgacccc cagcgtggcc gagcgcaccg cctcgtcgat gaggaccacc cccacggaca
       61 cgggacccgc gagcgcgccc ctgcccacct cgtccatccc cgcgaggacc cggtgaccgg
      121 cgcgctgcag cgtgcgctcg acccgaaggg acggccgtcg cgacggggtc ggccggccgg
      181 tcatgagccg ggcgtcgtcg tgaggccggc gttggggacc ttggcaaaga cgtcgtcctg
      241 gtgaccgagc caggtcatcc ggccgagcgg ccagacgagg gcgatcgcgc gaccggtcac
      301 cacgtcgatg gggaccgacc cggtccggcc ggtaccatcg tcgtggaagc gcgagtccga
      361 ggagtccgag cggtggtcgc ccatgaccca gatccgccct gccgggacgg tgatgtcgaa
      421 cggccgggtg ctggggtcgt cgcccgcctt gacgtagaac tcggtgaggg cgaccccgtt
      481 gaccttgatg ctccccgtgc cgtcggcctg gacgtggtcg ccgggcagcc cgatcacgcg
      541 cttgatgagg tggttcttgg cgtcgtccgg caatagaccg atgaaggtga ggacgcgctg
      601 ggtcgtgctc ggctcccccg cgacgctctg gccgagccac ggcgacggca ccttggggtc
      661 ctcgaagacc acgacgtcgc cgcgctcgag gtcgaacggc cctggcgtca gcttgctcac
      721 gatgacccgg tcgttcttga cgagggtgtc ctccatcgac tccgacggga tgtagaaggc
      781 ctggacgagc caggtcttga tgacgaagga gatcagtgcg gccatgatga cgacgaggac
      841 gacctcgcgc accgtcgcca cgatgcgctg accgacgctc atcggggcgg gagcaccgcc
      901 gtggcgacca cggcgcacct gcgtcgacgc cgcggacgcc gcaccgttgg tctggtcagc
      961 catgaggcgc tgtctctcct gccgttcgcc gcagccctca gccaccgggg cccccgagcc
     1021 tgtccagggg ccagtatcgc acccagacct ggccgatcac gtccgtagcg gcgacggtgc
     1081 caccgcccgg tcccccgagg tgtgctcggg agtcctcgga ctcgccccga tggtctccga
     1141 gaacgaagac ccgacccgct gggaccagga tgtcgaacgg gctggcactg gccggttccc
     1201 ccgggaagag atacggctcg gccacggcca cgccgttgac gctgagccgc ccctcctgcg
     1261 tgcaacagcg gacccggtcg ccgcctaccg ccaccacccg cttgacgtag tccccgtcgg
     1321 tgccgccgcc gaacgccgcc gcaccgtcga agacgaccac gtcaccgcgc cggaggggac
     1381 caccgtgctg ccatcgggcg acgagcaccc ggtcgccggg ctggaacgac ggctgcatcg
     1441 aggtcgaggt gagcacgaac gggttcacga cgaaggcccg gaccagcgcg agcaccagga
     1501 cggtcacggc cagtgcac
//
LOCUS       DATAIX010000325         1968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_107103, whole genome shotgun sequence.
ACCESSION   DATAIX010000325 DATAIX010000000
VERSION     DATAIX010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107103"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="VHM65_04850"
     CDS             complement(<1..1271)
                     /locus_tag="VHM65_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705066.1"
                     /translation="MVATLRGTPGHDIVELGLTALRNLDHRGATGADPLVGDGAGILT
                     QIPDGFLRASVGFALPIAGAYAAGMAFLPRDETRRAEVVAVIERIAREESLDVLGWRE
                     VPVATDLVGQVARDCMPYFAQLFVSSSIGRAVGLGLDRLAFCLRKRAERETDVYFASL
                     SGRTIVYKGMLTTSQLEPFFPDLSDHRFTTELAIVHSRFSTNTFPSWPLAHPYRLIAH
                     NGEINTVKGNRNWMRARESLLTSDIIPGAIARIFPICTPGGSDSASFDEVLELLHLGG
                     RSLPHAVLMMIPEAWENHTSMDPARSAFYQFHSTFMEPWDGPACVTFTDGTLVGAVLD
                     RNGLRPGRYSVTDDGLVVLASESGVLNLDPARVVKKGRLQPGRMFLVDTEHGRIISDD
                     EIKGTLAAAHPYDEWLHAGLIKLEDLPDREH"
     gene            complement(1488..>1968)
                     /locus_tag="VHM65_04855"
     CDS             complement(1488..>1968)
                     /locus_tag="VHM65_04855"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX2705067.1"
                     /translation="FLDSAAPGVLVAQALGRFGNYFNNEIHGGPTSLPWGLQVYEWDA
                     AAGHAVRDAFGNPVVAGVFHPTFLYEAIFCVALAAVIIGVERRRSLRPGQVFALYVMG
                     YPVGRGLIELMRTDDANRILGLRLNVWTSVLVFFLGLSLFRRAARSHDPDRLSHDVI"
BASE COUNT          331 a          619 c          744 g          274 t
ORIGIN      
        1 atgtgctccc ggtcaggcag gtcctcgagc ttgatgagcc cggcgtgcag ccactcgtcg
       61 tacggatgcg ccgcggccag cgtgcccttg atctcgtcgt cgctgatgat ccggccgtgc
      121 tcggtgtcga cgaggaacat ccggccgggc tggagccggc ccttcttgac cacccgagcc
      181 gggtcgaggt tgaggacccc ggactcggag gcgaggacga cgagcccgtc gtcggtgacc
      241 gagtagcgtc ccgggcgcag tccgttgcgg tccaggacgg cgccgacgag ggtcccgtcg
      301 gtgaaggtca cgcaggccgg accgtcccac ggctccatga aggtcgagtg gaactggtag
      361 aaggccgatc gggccgggtc catcgacgtg tggttctccc acgcctcggg gatcatcatg
      421 aggacggcgt gcggcaggga ccggccaccg aggtggagca gctcgagtac ctcgtcgaac
      481 gaggccgagt ccgagccacc gggcgtacag atcgggaaga tccgcgcgat ggcgcccggg
      541 atgatgtccg aggtcagcag gctctcgcgg gcccgcatcc agttgcggtt gcccttgacg
      601 gtgttgatct cgccgttgtg cgcgatgagc cggtaggggt gcgccagcgg ccaggacggg
      661 aaggtgttgg tcgagaaccg ggagtggacg atggcgagct cggtcgtgaa ccggtggtcg
      721 gacaggtcgg ggaagaacgg ctcgagctgg gacgtcgtca gcatcccctt gtagacgatc
      781 gtgcgcccgg acagtgaggc gaagtagacg tcggtctcgc gttcggcgcg cttgcgcagg
      841 cagaaggcga ggcggtccag gcccagtccc acggcgcggc cgatcgagga ggacacgaac
      901 agctgggcga agtacggcat acagtcgcgg gcgacctgtc ccacgaggtc cgtggcgacc
      961 gggacctcgc gccagccgag gacgtcgagg ctctcctcgc gggcgatccg ctcgatgacc
     1021 gcgacgacct cggcccggcg ggtctcgtcc cggggcagga aggccatccc cgcggcatag
     1081 gcgccggcga tcggcagcgc gaagccgacg ctggcgcgca ggaacccgtc agggatctgc
     1141 gtcaggatcc cggccccgtc accgacgagc gggtcggccc cggtggcgcc gcggtggtcg
     1201 aggttgcgca gggccgtcag gccgagctcg acgatgtcgt gacccggggt cccgcgaagg
     1261 gtggccacca tcgcgacacc gcatgcgtcg tgctcgttct cccggcggta caggcccgcg
     1321 tcagcgggga aggcggagaa gaggggtctc ggcgagggaa tgggatcacc gtcctgggcg
     1381 tgacgtcgcg agcacgagct cggtgggggc gtagtaactg gacgacgtcg gccagcggct
     1441 ggtcgaagaa taaccgagcc tgctcgacca ccgctatgag gcgcgcctca gatcacatcg
     1501 tgagacaacc tgtccggatc gtggctgcgg gcggcccggc ggaagaggga cagcccgaga
     1561 aagaagacga gcaccgaggt ccagacgttg aggcgcagcc cgagaatgcg gttggcgtcg
     1621 tcggtgcgca tgagctcgat gagccctcga ccgacggggt agcccatgac gtagagcgcg
     1681 aacacctggc cgggtcgcag gctccggcgc cgctccaccc cgatgatgac ggcggcgagc
     1741 gcgacacaga agatcgcctc gtagaggaac gtcgggtgaa agacccccgc cacgacgggg
     1801 ttgccgaagg catcacgcac cgcgtggccg gctgcggcgt cccactcgta gacctgcagg
     1861 ccccagggca ggctcgtcgg gccaccgtgg atctcgttgt tgaagtagtt gccgaaccgt
     1921 cccagcgcct gggcgaccag cactcccgga gccgcggagt cgaggaag
//
LOCUS       DATAIX010000326         1759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_216154, whole genome shotgun sequence.
ACCESSION   DATAIX010000326 DATAIX010000000
VERSION     DATAIX010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216154"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VHM65_04860"
     CDS             complement(<1..851)
                     /locus_tag="VHM65_04860"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HEX2705068.1"
                     /translation="MPDSGSGAGPRTGIRADLLSDTLTRPTPAMRAAMASAEVGDDVF
                     GEDPTVRVLEERVAALLGHEAGLFTPSGSLANQLALRLHVRPGDELVADSLAHVVRAE
                     MGAAAVFSGITSRTWSAPRGLLSAATALDLVVTGVGPYQVCTSLVVVENTHNFGGGTV
                     QPLEQIQALREGTAGRGVAMHLDGARLANAHVATGVPFASYGQLFDTVSLCLSKGLGA
                     PVGSVLVGSVERVAEARVWRKRYGAGMRQVGILAAAGVYALDHHVQRLAEDHARAARL
                     AAALHDVA"
     gene            complement(865..>1759)
                     /locus_tag="VHM65_04865"
     CDS             complement(865..>1759)
                     /locus_tag="VHM65_04865"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF013628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX2705069.1"
                     /translation="TPDPQRLVRAYHASSATLNLVRAFTMGGFADLRMVHDWNKGFVS
                     NGANMRYERLARDIDKAMRFMAACGADFDAMRTTEFFAAHEALLLDYERPLTRIDSRT
                     GLPYDTSAHFVWVGERTRELDGAHIDYVSRVRNPIGVKVSPRAEPDYLLKLIDQVDPD
                     REPGRLTFITRMGAGTIRESLPPIVEQVTASGAEVTWICDPMHANTFESTSGYKTRHF
                     DDVVEEVLGFFEVHRGLGTVPGGLHVELTGNDVTECLGGAEKILDADLEKRYETVCDP
                     RLNHQQALELAFLVAEMLSRD"
BASE COUNT          253 a          640 c          623 g          243 t
ORIGIN      
        1 gcgacatcgt gcagcgccgc cgcgagccgg gccgcgcgcg catggtcctc ggccagccgc
       61 tggacgtggt ggtcgagcgc gtacaccccc gcggcggcga ggatgcccac ctggcgcatg
      121 cccgcgccgt acctcttgcg ccacacgcgt gcctcggcga cccgctccac cgacccgacg
      181 aggaccgagc cgaccggggc gccgagcccc ttgctcaggc acagcgacac cgtgtcgaag
      241 agctgcccgt atgacgcgaa cggcaccccc gtcgccacgt gggcgttggc caggcgcgcg
      301 ccgtcgaggt gcatcgcgac cccgcggccg gcagtcccct cccgcagagc ctggatctgc
      361 tccagcggct ggaccgtccc gccgccgaag ttgtgcgtgt tctcgacgac gacgaggctc
      421 gtgcagacct ggtagggacc gaccccggtg acgacgaggt cgagcgccgt ggcggcggac
      481 aggagccccc gcggcgccga ccaggtccgc gaggtgatcc ccgaaaagac cgccgccgcg
      541 cccatctcgg cccgcaccac gtgggcgagc gagtcggcga cgagctcgtc accgggccgg
      601 acgtgcagcc gtagcgcgag ctggttggcg agggacccgc tgggggtgaa gagcccggcc
      661 tcgtggccga gcagagccgc cacccgttcc tcgaggaccc gcaccgtcgg gtcctcgccg
      721 aagacgtcgt cgcccacctc ggccgaggcc atcgcggccc gcatggccgg cgtcgggcgg
      781 gtcagggtgt ccgagagcag gtcggcgcgg atcccagtgc gcgggccggc gcctgagccg
      841 gagtcgggca cggcgagcag ccctctagtc ccggctgagc atctcggcga ccaggaaggc
      901 cagctcgagc gcctgctggt ggttgagccg cgggtcgcac acggtctcgt agcgcttctc
      961 caggtcggcg tcgaggatct tctccgcccc cccgaggcac tcggtcacgt cgttgccggt
     1021 cagctcgaca tgcaggccgc ccggcacggt gcccaggccg cgatggacct cgaagaagcc
     1081 gagcacctcc tcgacgacgt cgtcgaaatg ccgggtcttg tagcccgagg tcgactcgaa
     1141 cgtgttggcg tgcatggggt cgcagatcca ggtgacctcg gcgcccgagg cggtgacctg
     1201 ctccacgatc ggcggcagcg actcgcggat cgtgcccgca cccatcctcg tgatgaaggt
     1261 cagccgccct ggctcccggt cggggtcgac ctggtcgatc agcttgagga ggtagtccgg
     1321 ctcggccctc ggcgagacct tgaccccgat cgggttgcgc acccgcgaga cgtagtcgat
     1381 atgcgccccg tcgagctcgc gcgtgcgctc ccccacccag acgaagtgcg ccgacgtgtc
     1441 atagggcagg ccggtccgcg agtcgatccg ggtcagcggc cgctcgtagt cgagcagcag
     1501 cgcctcgtgc gcagcgaaga actcggtcgt gcgcatcgcg tcgaagtcgg cgccacaggc
     1561 cgccatgaac ctcatcgcct tgtcgatgtc cctggcgagg cgctcgtagc gcatgttcgc
     1621 accgttgctg acgaagccct tgttccagtc atggaccatc cgcaggtcgg cgaagccgcc
     1681 catcgtgaac gcccgcacga ggttcagcgt cgccgacgac gcgtggtagg cccggacgag
     1741 ccgctgcggg tcgggggtc
//
LOCUS       DATAIX010000327         1897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_245996, whole genome shotgun sequence.
ACCESSION   DATAIX010000327 DATAIX010000000
VERSION     DATAIX010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245996"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /gene="ispG"
                     /locus_tag="VHM65_04870"
                     /gene_synonym="gcpE"
     CDS             <1..1140
                     /gene="ispG"
                     /locus_tag="VHM65_04870"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX2705070.1"
                     /translation="MPGVPQAVVAPRRVSRKIRVGKVEVGGDAPVSVQSMTTTPTTDV
                     DATLQQIAELTATGCDIVRVACPTQDDADALAAIAKHSQLPVIADIHFQPKYVFAAID
                     AGCAAVRVNPGNIRQFDDQVGEIARAAHDAGVSIRIGVNAGSLDPRLLKKYGKATPEA
                     LVESAVWEASLFEEHDFHDFKISVKHNDPVVMIRAYEQLAARGDWPLHLGVTEAGPAF
                     QGTIKSSVAFGALLAKGIGDTIRVSLSAPPVEEVKVGNQILQSLGLRPRKLEIVSCPS
                     CGRAQVDVYTLADQVTAGLEGLEVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIF
                     VRGEVIKTVPESQIVETLIEEAMRLAEEMGETADGEAGSPMVTVS"
     gene            complement(1474..>1897)
                     /locus_tag="VHM65_04875"
     CDS             complement(1474..>1897)
                     /locus_tag="VHM65_04875"
                     /inference="COORDINATES: protein motif:HMM:NF023011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3238 domain-containing protein"
                     /protein_id="HEX2705071.1"
                     /translation="GSTHSIEITKNVGQTIAYGCTTPWGTDCVAIQSATAPDSELSWI
                     NVSSGNTTADFTLVAAAKNPLVSIAPTIDAGVAFHLRPGGTTVRGIHDRMPVHEIYFY
                     VDGWGEWEPVYRSDAWFLPCLGGYAVAPGCETKFSVLL"
BASE COUNT          309 a          658 c          637 g          293 t
ORIGIN      
        1 atgcctgggg tgccccaggc cgttgtcgcc ccccgccggg tctcccgtaa gatccgggtc
       61 ggcaaggtcg aggtcggcgg cgacgccccc gtctcggtcc agtcgatgac cacgacgccg
      121 acgaccgacg tcgacgcgac cctgcagcag atcgccgagc tgacggccac cggctgcgac
      181 atcgtccggg tggcctgccc gacccaggac gacgccgacg cgctcgcggc catcgccaag
      241 cacagccagc tcccggtcat cgccgatatc cacttccagc ccaagtacgt cttcgcggcc
      301 atcgacgccg gatgtgccgc cgtgcgggtc aacccgggca acatccgcca gttcgacgac
      361 caggtcggtg agatcgcccg ggccgcccac gacgccggag tgtcgatccg catcggggtc
      421 aacgcggggt cactcgaccc gcggctgctg aagaagtacg gcaaggccac gcccgaggcg
      481 ctcgtcgagt cggcagtctg ggaggcaagc ctgttcgagg agcacgactt ccacgacttc
      541 aagatctcgg tcaagcacaa cgacccggtc gtcatgatcc gggcctacga gcagctcgcc
      601 gcgcggggcg actggccgct gcacctcggc gtgaccgagg ccggcccggc cttccagggg
      661 acgatcaagt cctcggtggc cttcggggcg ctgctcgcca aggggatcgg cgacacgatc
      721 cgcgtctcgc tgtccgcccc tcccgtcgag gaggtcaagg tcggcaacca gatcctgcag
      781 tccctcggtc ttcgcccgcg caagctcgag atcgtgtcct gcccctcgtg tgggcgcgcc
      841 caggtcgacg tctataccct ggccgaccag gtgaccgccg gcttggaggg cctcgaggtg
      901 cccctacggg tcgcggtcat gggctgcgtc gtcaacggcc cgggcgaggc tcgcgaagcc
      961 gacctcggcg tcgccagcgg caacggcaag ggccagatct tcgtccgcgg cgaggtcatc
     1021 aagaccgtcc ccgagtcgca gatcgtcgag accctcatcg aggaggccat gcgcctcgcc
     1081 gaggagatgg gcgagacggc cgacggcgag gcgggctcgc ccatggtcac ggtcagctga
     1141 cgcctggccc gggtccgctc ggccgacccg ctcatgacga ctcggggtgc cgacgcgatg
     1201 gggcggctac ctcggcactg ttccggtcac cacgggacga atcgggcgcc cgacgcgtcg
     1261 ggagcgacac gctaacgacg cgtcattgca gcagggccgc acccaaatgg ataggccgga
     1321 ccctgacaac cagggggtga ttgctcgacg ctgcttgacc gacggactcg ctatccccac
     1381 cggccccggt tcgtcggagg gttcgtgctg ctctacgggc agccgagcgt tggcgcgagt
     1441 acgccagtgg ggcgtgggcc ccgtacgcgt cgtctacagg agaacgctga atttcgtctc
     1501 gcaccccggg gcaactgcgt agcctccaag acagggcaag aaccacgcat cgctgcggta
     1561 gacgggctcc cactcgcccc atccatcgac atagaagtag atctcgtgca cgggcatacg
     1621 gtcgtgaatt ccgcgaacgg tggtgccacc tggcctcagg tggaaggcca ctccggcatc
     1681 gatggtcggc gcgatgctga ccagcgggtt ctttgccgcc gccaccagag tgaagtctgc
     1741 ggttgtgttt ccgctgctca cgttgatcca agatagctca ctgtcgggag cggtcgccga
     1801 ctgaatggcg acgcagtcag tgccccacgg cgtggtgcag ccgtaggcga tcgtctggcc
     1861 gacattcttg gtgatctcga tcgaatgcgt ggagccg
//
LOCUS       DATAIX010000328         1729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_47600, whole genome shotgun sequence.
ACCESSION   DATAIX010000328 DATAIX010000000
VERSION     DATAIX010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47600"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1069
                     /locus_tag="VHM65_04880"
     CDS             128..1069
                     /locus_tag="VHM65_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2705072.1"
                     /translation="MTTPAVSVRSLRKAYGSTLAVRDVSFDIDAGEIVGILGPNGSGK
                     TTTVECLQGLRRADAGQVDVLGLDPRTQLSALRRRVGSQLQDSALPARIRVEEAVRLF
                     ASVGAGDCDIPVLLRDWGLAHRRRAAFGSLSGGERQRLFVALALVNGPELVFFDEMTT
                     GLDPAARREVWGLVERVRDLGTTVVLVTHFMDEAERLCDRLVVLRDGLVVATGTPAEL
                     VAAHGGGVTAVFSSVVVDDRTLAELPGVRAVHRRGATVEVTGEARFVLHLGHLLVSES
                     AVPDDLRIRQPSLEDAFFRLVDAGEKSPPRPQDEGSP"
     gene            1066..>1729
                     /locus_tag="VHM65_04885"
     CDS             1066..>1729
                     /locus_tag="VHM65_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2705073.1"
                     /translation="MKGRVLVESSRVEFLLWLRDPITLVFALALPVLNVAVLAGVFGD
                     QPDPSGEVFRGVGGSTYYTPAYIALVAASVGLIAVPTQLAEYRDQGVLRRFRSAGLPV
                     WAVLGGQIVVGLALSVVGAAFVVALSFIAYEPVGPDHPGGVAVAFLIGTVAFLMLGLM
                     LGCLLPTARAAQDASALGGPPPEVLPDIMHTLGSLTPLHPMVVALQDPWFGDGWNTSM
                     LAV"
BASE COUNT          202 a          616 c          608 g          303 t
ORIGIN      
        1 gttgcaggaa tgccccacga tgcccgacct ctgcgcccgt gagtggcgca tcgcgcgtgg
       61 catgcgacgt gccggggtgt cttccggccc tggccacgca atcccgcgct cggatacggt
      121 cgtcgccatg acgacgcccg ccgtgtctgt tcgttccttg cgcaaggcct acgggtccac
      181 gctggcggtc cgagatgtgt cgttcgatat cgatgcgggc gagatcgtcg ggatcctggg
      241 gccgaacggg tccggcaaga cgaccaccgt cgaatgcctg cagggcctgc gacgtgcgga
      301 cgccgggcag gtcgacgtcc tgggtctcga cccgcgcacg cagctgagcg cactgcggcg
      361 ccgggtcggt agccagctgc aggactcggc gctacctgcg cggatccggg tcgaagaagc
      421 ggttcggctg ttcgcctccg tcggggccgg cgactgcgac atcccggtcc tgctgcggga
      481 ctggggcctg gcccaccggc ggcgggcggc atttggcagc ctgtccgggg gcgagcgtca
      541 gcgactgttt gttgcgctgg ccctggtgaa cggcccggag ctggtcttct tcgacgagat
      601 gacgaccggc ctcgacccgg ccgcgcgcag ggaggtgtgg ggcctcgtcg aacgggtccg
      661 cgacctgggc accaccgtgg tcctggtcac ccatttcatg gacgaggccg aacggctctg
      721 cgaccggctc gtggtgctcc gtgacggcct tgtggtcgcc accggcaccc cggccgagct
      781 ggtcgcagcc cacggcggcg gggtgaccgc cgtcttctcg tccgttgtcg tcgacgaccg
      841 cacgctcgcc gagctgcccg gcgtccgggc cgtgcaccgg cgcggggcca cggtcgaggt
      901 caccggtgag gcgcgtttcg tcctgcacct cgggcacctc ctcgtctccg agtccgcggt
      961 tcccgacgac ctgcgaatcc gccaaccgtc actggaggat gccttcttcc ggctcgttga
     1021 cgccggtgag aagtcgcctc cccgcccgca ggacgaggga tcgccgtgaa gggccgcgtc
     1081 ctggtcgagt cgagccgggt cgagttcctg ctgtggctgc gcgacccgat caccctggtc
     1141 ttcgcgctgg cgctccccgt gctcaacgtc gccgtgctcg ccggtgtctt cggggaccag
     1201 ccggacccct ccggtgaggt cttccgcggc gtggggggtt cgacgtacta caccccggcc
     1261 tacatcgcgc tggtcgccgc ctcggtcggc ctgatcgccg tgcccaccca gctcgccgaa
     1321 taccgcgacc agggtgtgct gcgaaggttc cgttcggcgg ggctgccggt gtgggccgtc
     1381 ctgggcgggc agatagtcgt gggtctggcc ctatccgtgg tcggtgccgc cttcgtcgtg
     1441 gcgctgtcgt tcatcgccta cgaaccggtc ggcccggacc atcccggggg cgtggccgtg
     1501 gccttcctga tcggcaccgt ggcgttcctc atgctgggac tcatgctcgg ctgcctcctg
     1561 ccaaccgccc gggccgccca ggacgccagc gccctgggcg gcccgccacc ggaggtgttg
     1621 cctgacatca tgcacaccct cggctccctg acacccctgc acccgatggt cgtcgcactc
     1681 caagacccct ggtttggcga cggatggaac acgagcatgc tggcagtgc
//
LOCUS       DATAIX010000329         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_17491, whole genome shotgun sequence.
ACCESSION   DATAIX010000329 DATAIX010000000
VERSION     DATAIX010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17491"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..401
                     /locus_tag="VHM65_04890"
     CDS             117..401
                     /locus_tag="VHM65_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX2705074.1"
                     /translation="MITAIVLVSTEVDAIPEAAAAIADLDGVTEVYSVTGNADLIAMV
                     RVRAHEELADVIADRLSKVPGVLATTTHIAFRTYSRHDLEAAFSLGLDGD"
     gene            complement(541..1593)
                     /gene="trpD"
                     /locus_tag="VHM65_04895"
     CDS             complement(541..1593)
                     /gene="trpD"
                     /locus_tag="VHM65_04895"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX2705075.1"
                     /translation="MAAEGQLTWPTLFTALLRDEHLDAASASWAMGQIMAGDATPAQV
                     AGFLMALRAKGETVEEMRGLADQMLAHALPAQVEGPTLDIVGTGGDRAHTVNISTMSA
                     MVAAAGGVRVVKHGNRAASSASGSADVLEALGIDLSLPAERVAAVAAEAGITFCFAQV
                     FHPSMRHAAAARRELGIATAFNFLGPLTNPAQPTYAAVGVADARMAPLLAGVFAGRGK
                     DAAVFRGDDGLDEVTLSTTTRVWWVRAGRVSERTLDPQRLGVALAPVEALRGGDAAHN
                     AAVVRSVFGGALGPVRDAVVLNTGLALALTSRDSGPGRDAFDTDLRAGMARAARALDS
                     GGAAALLARWVQATRS"
     gene            complement(1598..2020)
                     /locus_tag="VHM65_04900"
     CDS             complement(1598..2020)
                     /locus_tag="VHM65_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2705076.1"
                     /translation="MSATTATPVRQIRVLLYSDDVTTRDAVRLAVGRRPARDVEVQSW
                     LECASAAAVVEAVEAGGLDVLVLDGEAAPLGGLGLCRQLKNEIFNCPPVLVLTGRPQD
                     GWLAAWSQADLAVPHPLDPIIVAAAVATLSRVGAPTPA"
     gene            2122..>2436
                     /locus_tag="VHM65_04905"
     CDS             2122..>2436
                     /locus_tag="VHM65_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705077.1"
                     /translation="MATASALPNDLTRAVPGHGPVTRPNMASVGTIVWLSSELMFFAG
                     LFAMYFQIRSLSAPLWAESTAKLNITYAAANTLILVISSVWCQMGVFAAERGQPGRSG
                     TLL"
BASE COUNT          364 a          907 c          849 g          316 t
ORIGIN      
        1 gtggtatgac gccccggccc gttccgatcg gctccgccag actctggtcc gcccgccacg
       61 ccctcggctg gcgacaccgc caccactggc gacagggcca ccagtagtct ggggccgtga
      121 tcactgccat cgtcctggtc agcaccgagg tcgacgccat ccccgaggcc gcagccgcca
      181 tcgccgatct tgacggcgtg accgaggtct actcggtcac cggcaacgcc gacctcatcg
      241 ccatggtgcg tgtgcgggcc cacgaggagc tcgccgacgt catcgccgac cgcctgagca
      301 aggtccccgg ggtcctggcg acgacgaccc acatcgcgtt ccggacctac tccaggcacg
      361 acctcgaggc ggcgttcagc ctcggcctcg acggcgactg acccggcgcc caccaggcga
      421 cccgagctga agcacagcgc gcacaggctg ggtcacgaca cgacgactgc aaccgtgccg
      481 acgtgaccca acccctgcga tgtcggcggc ggccagtgcc gaggccggtg ccagtgaggt
      541 tcaggagcgg gtggcctgga cccaccgggc gagcagcgcc gccgcaccgc cggagtcgag
      601 cgcccgcgcc gcgcgcgcca taccggctcg caggtcggtg tcgaaggcgt cgcggcccgg
      661 cccgctatcc cgggacgtca gggccagcgc gagccccgtg ttgagcacga cggcgtcccg
      721 gacgggaccg agcgccccac cgaagaccga acggacgacg gcggcgttgt gggccgcgtc
      781 accgccgcgc agggcttcga ccggggccag ggcgacaccc agtcgctgcg ggtccagcgt
      841 ccgctcgctg acccggcctg cgcgcaccca ccacacgcgg gtagtcgtcg acagggtgac
      901 ctcgtcgagg ccgtcgtcac cgcggaagac tgcggcgtcc ttgcctcgac cggcgaacac
      961 cccggcgagc agcggcgcca tccgggcatc ggccaccccg accgcggcgt aggtgggctg
     1021 ggccgggttg gtcagtgggc cgaggaagtt gaaggccgtc gcgataccga gctcacggcg
     1081 agcagccgcg gcgtgacgca tcgagggatg gaagacctgc gcaaagcaga aggtgatgcc
     1141 ggcctctgcc gccacggcgg cgacccgctc ggccggcagg ctcaggtcga tcccgagcgc
     1201 ctcgaggacg tcggccgacc ccgaggccga cgatgcggcc cggttgccgt gcttgaccac
     1261 gcgcacaccg ccggcggcgg ccaccatggc cgacatcgtc gagatgttga cggtatgggc
     1321 acggtcaccg ccggtcccca cgatgtcgag cgtcggtccc tcgacctggg ccggcagcgc
     1381 gtgcgccagc atctggtcgg ccaggccgcg catctcctcg accgtctcgc ccttggcgcg
     1441 cagagccatg agaaagccgg cgacctgtgc gggcgtggcg tcgccggcca tgatctggcc
     1501 catggcccag gaggccgacg ccgcgtcgag gtgctcgtcc cgcagcagcg cagtgaacag
     1561 tgtcggccag gtcagctggc cctcggcagc caccgcgtca ggcgggcgtc ggcgcgccga
     1621 cccggctcag cgtggccacg gccgcggcaa cgatgatcgg gtcgagcggg tgcggcacgg
     1681 cgaggtcggc ctgggaccag gcggcgagcc agccgtcctg cgggcgcccg gtcagcacga
     1741 gcaccggcgg gcagttgaag atctcgttct tgagctgacg acacaggccc aggccgccga
     1801 ggggcgccgc ctcaccgtca aggacgagca cgtcgagacc gccggcctcg accgcctcga
     1861 cgaccgcggc ggcgcttgcg cactcgagcc acgactgcac ctcgacgtcc cgcgccggac
     1921 gacggcccac cgcgaggcgc accgcgtcac gcgtggtgac gtcgtcgctg tacagcagga
     1981 cgcggatctg tcggaccggc gtggcggtgg tggcgctcat gccaggaatc gtagcgtccg
     2041 tcagacctcg ggccgcggct gcgagacagg tcaccggtcg gtcgcgcaga cgtagcgacc
     2101 ccagtaggcc ataatgctcc cgtggctacc gcatctgcgc tccccaatga cctgacccgc
     2161 gccgttcccg ggcatgggcc cgtgacgcgc ccgaacatgg cctcggtcgg caccatcgtg
     2221 tggctctcca gcgagctcat gttcttcgct gggctcttcg cgatgtactt ccagatccgg
     2281 tccctgtcgg caccgctctg ggccgagtcg acggccaagc tcaacatcac ctacgcggcc
     2341 gccaacaccc tgatcctcgt gatctcctcg gtgtggtgcc agatgggcgt gttcgcagcc
     2401 gagcgcggcc agcccggacg tagcggaacc ctgctc
//
LOCUS       DATAIX010000330         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_77662, whole genome shotgun sequence.
ACCESSION   DATAIX010000330 DATAIX010000000
VERSION     DATAIX010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77662"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..695
                     /locus_tag="VHM65_04910"
     CDS             72..695
                     /locus_tag="VHM65_04910"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX2705078.1"
                     /translation="MSSASRFLGAGVALGLVAGLLVGVGLTQPGRAGAAAPSAAPSLA
                     SSGAVAGAPGALPPITTGGDTVTSSGTAIAYPNFSGSPGVAPDHTIVVTGLGQADLQS
                     DGSNQAAAQKTALAAALADAKALADAIAADTGLSISGVLSVSASVSPGFGVMPLVANG
                     SVTPPCVVAVPGSGPQANGKTVLPQPVCPPTYQQTLSVSVTVEYRVG"
     gene            complement(1430..>2025)
                     /locus_tag="VHM65_04915"
     CDS             complement(1430..>2025)
                     /locus_tag="VHM65_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705079.1"
                     /translation="FDRAASRIVLGAIAPVSLMLAGWWGSLGLFGAGPWIAPSALGGV
                     AIGLALDLTVLGTWLGSLFAQRMPALIAVGIFYSAIIYGMFMGFPVMNLSVGIAGGYV
                     VGRRAALVPGSRSRALHEARLVARVASLMLFVLCCGTAWLALGEATIATEVQSMLGLP
                     FTPSAAEIQALIVVGGVGLVVTEYAATILAAHWAASR"
BASE COUNT          315 a          737 c          682 g          291 t
ORIGIN      
        1 gtccgcgggc gcgtccgggg aggtgcgcgc ccggcgatcc cgcggggttc gcgaccaaag
       61 gagtacccgc catgtccagc gcatcacggt tcctcggtgc cggggtcgcc cttggcctcg
      121 ttgcgggcct gctcgtcggc gtgggcctga cccagccagg tcgggcgggg gccgcggcgc
      181 cctcggccgc cccgagcctt gcatccagcg gcgccgtggc cggggcaccg ggcgcgctcc
      241 cgccgatcac gacgggtggg gacactgtca catcatcggg cacggccatc gcctacccga
      301 acttctcggg ctccccgggc gtggcgcccg accacaccat cgtcgtcacg ggcctcgggc
      361 aggctgacct gcagagtgac ggctccaatc aggctgccgc ccagaagacc gcccttgctg
      421 ccgcgctggc cgacgcgaag gcgctggccg acgccatcgc cgcggacacc gggctctcga
      481 tcagcggggt gctgtcggtg agcgcctcgg tgtcacccgg tttcggggtc atgccgctcg
      541 tcgccaacgg atcggtgaca ccgccctgcg tcgtggccgt gcccgggagc gggccgcagg
      601 cgaacgggaa gacggtcctg ccgcagccgg tctgcccgcc cacctaccag cagaccctca
      661 gcgtgtcggt gaccgtggag taccgggtcg gctgagggtg cagcggccga ccctgcgcct
      721 ccttggcgtg cgcccgggca acgccaccgt cctgctcgag ggtagcgcca cgggtcgacc
      781 ggggccgtcc ttatgcttcg tgatgggatg gcgctccccc tcgtcgtcgt gggtgaccct
      841 gctccttgcc gccaccatgg cggtaatgct gatcgctcct gtgtccgtat cggcgaacaa
      901 cgacccgcac tgctggtata tgggctcaga cccgttcgtc tgcgtcggga aaccgctaac
      961 cgtgccgcct gcgcttgtcg tccctcctgg cgatccccac cagacgctat tccctggccg
     1021 gcaccggcgc actcgccaga ctggtctacg cgatctccga cgcccgagga acatagctcg
     1081 gaacgttcgt taccgggctc agcaaggatg gtgaggtgtg tcggctctaa cctctcgccc
     1141 cgaccagatc ctccagctcg gcgatttcgt gcttggattc cgccccggga cccgcctcgc
     1201 gacgcgggca tcttgcaccg acgtacctca acgtcccggt cgctgcggca gagtggcagc
     1261 ggagcgtgtg caagagtggt actagacgac gtgcgagagt ggtcgtctac agacatggac
     1321 tacctcccca ggctcgtcga cgggcagatc gacgcgctgc tggccgagct tcctgcaatc
     1381 tgctctcggc cccgcgcgga gcggcggctc cagcccggtg agacgccgcc taccgagatg
     1441 cggcccaatg cgccgcgaga atcgtcgcgg cgtactcggt gaccacgagc ccgacgcccc
     1501 cgaccacgat cagggcctgg atctcggcgg ccgacggcgt gaagggcagg cccaacatgg
     1561 actgaacctc ggtcgcgatc gtcgcctcgc cgagagcgag ccatgcggtc ccgcagcaca
     1621 gcacgaagag catgagggat gcgacgcggg caacgaggcg ggcctcatgg agcgcccggg
     1681 accgcgaacc ggggacgagc gcggcccggc gaccgaccac gtagcccccg gcaatcccca
     1741 ctgacaggtt catgaccggg aaacccatga acatgccgta gatgatcgcg gagtagaaga
     1801 tccccactgc gatcagggcc ggcatacgct gggcgaacag cgagccgagc cacgtgccca
     1861 gcaccgtcag gtcgagcgcc agcccgatgg cgacgccacc aagcgcactc ggggcgatcc
     1921 acggaccggc cccgaacagg ccgagcgatc cccaccagcc agccagcatg agcgagactg
     1981 gcgcgatcgc gcccagcact atgcgcgatg ccgccctgtc gaagc
//
LOCUS       DATAIX010000331         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_77720, whole genome shotgun sequence.
ACCESSION   DATAIX010000331 DATAIX010000000
VERSION     DATAIX010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77720"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..1361)
                     /locus_tag="VHM65_04920"
     CDS             complement(258..1361)
                     /locus_tag="VHM65_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HEX2705080.1"
                     /translation="MIRWRRATVTEVLPSWPGVCLYDVTLASGASLRALAYTDVVGEP
                     LVGDVVLLNTTALDAGLGTGGVALVVAVPDRIPDTAGSGPGHLVKARYTPLQTMVLGA
                     DEPDSPHHGVLREASDLGGLPVVVADLHSALPAIVAGLRAERPQARVAYVMTDGGALP
                     LAFSRTVAALRSHDWLVGCVTVGQAYGGDLEAVTVHSGLLAARHVLGADVAVVTQGPG
                     NLGTDTPWGFSGVGAGEALNAAVTLGGVGVASLRVSQADARDRHRGISHHSVTAYGLV
                     ALVAADIPVPLLGGPIGSQVLREARALTAASDGRLRLHEVDVDGLDAVLRECPVPLRT
                     MGRSYDEDRAGFLAVAAAGRYVGRSLPASPPAA"
     gene            1453..>2099
                     /locus_tag="VHM65_04925"
     CDS             1453..>2099
                     /locus_tag="VHM65_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX2705081.1"
                     /translation="MSAQTGAAAKTERLLNLVICLLSTRMPLTKARIRQAVPQYQSTA
                     SVEAFDRMFERDKDELRDLGIPLSTDVIDPLFDDEVGYRIDRREYALPETHFEPDEVA
                     ALALAGRVWTQASLAGAASMALRKLRAAGVEIDDRSLVDIEPRVRTAEAAFEPMRRAL
                     LARAPVRFAYRKSGGEPEPRTLRPWGLTSWHGRWYVTGYDVERGAERVFRLDRVEG"
BASE COUNT          314 a          759 c          780 g          246 t
ORIGIN      
        1 gtcgtcgcga gcagaccgtg gccttctcgg gctggttgcg tgggttgttg aggtggatgt
       61 ggccggtgct gaccggccgg atccacctcg gtaagcggcc cccgcgtcga cgcggccggt
      121 caacaagggc aggaacaggt cagccatggg cgcggtgccg cggcgatggg cccgggcggg
      181 atctgggagg gttggcaggg tcaggacccc accaaccctc ccagtcagta tgggagcccc
      241 gtccggtcgc gacagggtca ggccgccggc ggcgaggccg gcagggaacg gccgacgtac
      301 cggccggcgg cagcgacggc gaggaagccg gcccgatcct cgtcatacga gcggcccatg
      361 gtgcgcaacg ggacagggca ttcgcgcagg acagcgtcga ggccgtcaac gtcgacctcg
      421 tgcagccgca gccgcccgtc cgaggccgcc gtcagcgcgc gggcctcccg gagcacctgt
      481 gagccgatcg ggccgcccag caagggaact gggatatcgg cggccaccag cgcgacgagc
      541 ccgtaagccg tcacactgtg gtgcgagatc ccccggtgcc ggtcgcgggc gtcagcctgc
      601 gacacccgca gcgaggccac gccgacaccg cccagcgtga cggcagcatt gagcgcctcg
      661 ccggcgccca cgccggagaa cccccacggg gtgtccgtgc cgaggttgcc gggcccctgg
      721 gtcacgaccg ccacatcggc gccgaggacg tgccgggccg cgagcaggcc gctgtgcacc
      781 gtgaccgcct cgaggtcccc gccatacgcc tggccgacgg tgacgcaccc cacgagccag
      841 tcgtgcgagc gcaaggcggc gacggtgcgc gagaacgcca ggggtagagc cccgccgtcg
      901 gtcatgacat aggccacccg ggcctgcggc cgctcggcgc gcagcccggc aacgatcgcc
      961 ggcagggccg agtgcaggtc ggccacgacg accggcaggc cgccgaggtc ggacgcctcg
     1021 cgcaggaccc cgtgatgggg gctgtccggc tcgtcggcac cgagcaccat cgtctgcaag
     1081 ggggtgtacc tggccttgac caggtgcccc gggccggagc cggccgtgtc cgggatgcgg
     1141 tcggggaccg ccacgacgag agcgacccct ccggtgccga gaccggcgtc gagggccgtc
     1201 gtgttgagca gcaccacgtc accgaccagc ggctcaccga ccacgtcggt gtaggccagc
     1261 gcgcgcagcg aggcccccga ggccagcgtc acgtcataca ggcacacacc gggccacgac
     1321 gggaggacct ccgtcacggt cgcgcgccgc cagcgaatca cccgggcaga ctatcggctg
     1381 aggtagcgtc ggggccgagg gcaggcgacc gtatccgtcc tgcgccgagc gtcccgaggg
     1441 aggcgaccga gcatgtccgc ccagacgggt gccgcggcca agaccgagcg cctgctcaac
     1501 ctcgtcatct gcctgctgtc cacccggatg ccgctgacga aggcacggat ccgccaggcg
     1561 gtcccgcagt accagagcac cgcctcggtc gaggcgttcg accggatgtt cgagcgcgac
     1621 aaggacgagc tgcgcgacct cggcatcccg ctgtcgacgg atgtcatcga cccgctgttc
     1681 gacgacgagg tcggctaccg gatcgaccgg cgcgagtatg ccctgcccga gacccacttc
     1741 gagcccgacg aggtcgccgc gctcgccctg gccgggcgcg tgtggaccca ggcgagcctg
     1801 gccggagcgg cgagcatggc gctgcgcaag ctgcgggccg cgggcgtcga gatcgacgac
     1861 cgttccctgg tcgacatcga gccgcgggtc cgcaccgccg aggcggcctt cgagccgatg
     1921 cgccgggcgt tgctcgccag ggcgccggtc cgcttcgcct accgcaagtc cggcggggag
     1981 ccggagcccc ggaccctgcg cccctggggg ctgacctcgt ggcacggccg ctggtacgtc
     2041 acggggtatg acgtcgagcg tggcgccgag cgggtcttcc gcctcgaccg tgtcgaagg
//
LOCUS       DATAIX010000332         2447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_47781, whole genome shotgun sequence.
ACCESSION   DATAIX010000332 DATAIX010000000
VERSION     DATAIX010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47781"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1264)
                     /locus_tag="VHM65_04930"
     CDS             complement(77..1264)
                     /locus_tag="VHM65_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEX2705082.1"
                     /translation="MAARRQLTERVLDLDTVLRDPATRTIVCCGSGGVGKTTTAAAMA
                     LRAAEQGRAVVVLTIDPARRLAQSLGLTELDNTPRPVVGVDIALGGSLDAMMLDMKRT
                     FDEVVIAHAAPDKAGQILENPFYQAVSSSFAGTQEYMAMEKLGQLRAELDAESGTPRW
                     DLIVVDTPPSRSALDFLDAPHRLGSFLDGRFIRLLMAPARVSSRAYLRFVSVGVHLVT
                     GAMTKILGGQFLTDVQTFVSALDTMFGGFRERADQTYRLLKEGGTSFVVVAAPERDAL
                     REASFFVDRLEQDGMPLAGLVVNRVQVVAAGTLSASRAWAAAEQLDDDDTAELPEHDA
                     LAVGLLRLHATLSEQADRQANLVRRFTAGHPEIATVQVPALAQDVHDLDGLRDVGRAL
                     APS"
     gene            complement(1265..2314)
                     /locus_tag="VHM65_04935"
     CDS             complement(1265..2314)
                     /locus_tag="VHM65_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEX2705083.1"
                     /translation="MSTPSPTLPVRWATTRLHIVTGKGGTGKTTVAAALALALAAEGK
                     RVLLAEVEGRQGISQVLDVAPLGLEETRLMGTLSGGEVVGLSVDAKAALLEYLQLFYK
                     LGRAGGALERFGAIDFATTIAPGVRDVLLIGKIYEANGRPSGGRAKNRGGPRAYDAIV
                     LDAPPTGRVVRFLSVSSEVAGLARVGPIKSQAESIMRMLTSRQTLVHLVTLLEDMPVQ
                     ETIDTIRDLDAVGLPVGAVIVNQVREDVLAPEGLDALERLVADPGAVDREAVRASMTR
                     AGIRVTGPMLDGLLREGADHAMRTRLAAEQWEVLAETGRPMLALPTLVDGVDANGVRE
                     LADWLGDLATAGAVR"
BASE COUNT          359 a          865 c          894 g          329 t
ORIGIN      
        1 ggcttggggg aacggatctg gggggccgtg tccgcggggg agcgctgcct gggggcagcg
       61 cgaccccgga ggccagtcac gacggagcga gcgcccggcc cacgtcccgc agtccgtcga
      121 ggtcgtgcac gtcctgggcc agggccggga cctgaacggt cgcgatctcc ggatgtccgg
      181 cggtgaaccg ccgcacgagg ttggcctgac ggtccgcctg ctcggacaac gtcgcatgca
      241 ggcgcagcag gccgaccgcc agggcgtcgt gctcgggtag ctccgcagtg tcgtcgtcgt
      301 cgagctgctc cgctgcggcc cacgctcggg acgccgacaa ggttccggcg gccacgacct
      361 ggacccggtt gacgacgagc ccggcaagag gcatcccgtc ctgttcgagg cggtcgacga
      421 agaacgacgc ctcccgcagc gcgtcacgct cgggcgcggc caccacgacg aacgacgtcc
      481 cgccctcctt gagcagccgg taggtctggt cggctcgctc ccggaacccg ccgaacatcg
      541 tgtcgagtgc gctgacgaac gtctggacgt cggtcaagaa ctgaccgccg aggatcttcg
      601 tcatggcgcc ggtaacgagg tggacgccca cgctgacgaa cctgaggtag gcgcggctac
      661 tcaccctcgc cggagccatg agcagcctga tgaagcgccc gtcgaggaac gacccgagcc
      721 ggtgtggtgc atcgaggaag tccagtgccg accgcgaggg cggagtgtcg acgacgatga
      781 ggtcccagcg cggggtgccg gactcggcgt cgagctcggc ccgcagctgt ccgagcttct
      841 ccatcgccat gtactcctgc gtcccggcga acgaggagga gaccgcctgg tagaacgggt
      901 tctccaggat ctggccggcc ttgtcggggg cggcgtgggc gatgacgacc tcgtcgaagg
      961 tccgcttcat gtcgagcatc atcgcgtcga gcgaaccgcc cagggcgatg tccacgccca
     1021 ccaccggcct gggggtgttg tcgagctcgg tgagcccgag cgactgggcg agccggcggg
     1081 cggggtcgat cgtgaggacc accaccgccc ggccctgctc ggccgcacgc aaggccatgg
     1141 cggccgccgt cgtcgtcttg ccgacccccc ccgagccgca acagacgatc gtgcgggtcg
     1201 ccgggtcacg caggacggtg tcgaggtcga ggacccgctc ggtgagctgt cgccgggcgg
     1261 ccacctacct caccgccccg gccgtcgcga ggtccccgag ccagtcggcg agctcacgga
     1321 ccccgttggc atcgaccccg tcgacgaggg tcgggagggc cagcatcggg cgccccgtct
     1381 cggcgaggac ctcccactgc tcggccgcga gccgggtgcg catcgcgtgg tcggcaccct
     1441 cgcgcagcag accgtcgagc atggggccgg tcacccggat ccccgcgcgc gtcatactcg
     1501 cccgcacggc ttcgcggtca actgcgcccg ggtccgcgac gagccgctcg agcgcgtcga
     1561 gcccttcggg ggccaggacg tcctcacgca cctggttgac gatgacggct ccgaccggca
     1621 gccccacggc gtcgaggtcc cgaatggtgt cgatcgtctc ctggaccggc atgtcctcga
     1681 gcaaggtgac gaggtggacg agcgtctggc gggaggtgag catccgcatg atcgactcgg
     1741 cctgggactt gatcgggccg acccgggcga gcccggccac ctcggagctg accgagagga
     1801 accgcacgac gcggccggtc ggcggcgcgt ccaggacgat cgcgtcatac gccctgggac
     1861 cgccacggtt cttggcacgc ccgccggacg ggcgcccgtt ggcttcgtag atcttgccga
     1921 tgagcaggac gtcccggacg ccgggggcga tcgtcgtggc gaagtcgatg gcgccgaaac
     1981 gctcgagcgc ccctccggcg cggccgagct tgtagaagag ctggaggtac tccagcagcg
     2041 cggccttggc gtcgacgctg aggccgacga cctcaccgcc ggacagtgtc cccatgaggc
     2101 gggtctcctc caggcccagc ggcgccacgt cgagcacctg actgatcccc tggcgcccct
     2161 cgacctcggc gagcaggacc cgcttgccct cggcggccag cgccagggcc aacgccgccg
     2221 cgacggtcgt cttgcccgtc ccgcccttac cggtgacgat gtgcaggcgg gtcgtggccc
     2281 accgaaccgg aagggtcggc gagggcgtgg acacgaagga cagcctaagg ggcggcgcgg
     2341 cgatgctgtc ggcgaccccg ggcgccctgg ggccccggag gcccgggcgc cctgggaagc
     2401 tgggggtaga gtccggccat gaccacgtgg gagtacgcga ccgttcc
//
LOCUS       DATAIX010000333         3276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_77733, whole genome shotgun sequence.
ACCESSION   DATAIX010000333 DATAIX010000000
VERSION     DATAIX010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77733"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1600
                     /locus_tag="VHM65_04940"
     CDS             <1..1600
                     /locus_tag="VHM65_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2705084.1"
                     /translation="RRVAAALTMFNLGADFRRRVTRQYLTLPLSWHHRHPSGQLLSNA
                     NADVEAMWQVFAPLPMALGVLVMLVVGVAQMVLVDPWLALIGLTVFPLLFAANSAFQR
                     AMSPRVTRAQQLRAQVSEVAHESFEAALVVKSLGREQLETDRFATVSDALRAAAVDVG
                     RTRGRFDPVIEAIPTIGTLAVLVVGTQRVVTGALTAADVVQIAYLFSILAFPVRALGW
                     VLGELPRSVVGWRRVSAVLQARGSTRYGARTLAGSGPAALRARGVSYAYDDAPGHDVL
                     RAVTLDVPAGGTVALVGPTGSGKSTLADVLLRLLDADRGTVWLDGTDTRELTREALTC
                     STALVAQETFLFDDTVLGNVTLGDVYTEDEVWAALRVAQAAEFVAALPQGLQTRIGER
                     GTSLSGGQRQRVALARAVVRRPRVLVLDDATSAVDPSVEQAILARVGESGATVLLIAY
                     RMATIALAEDVVYLEDGAVLDHGSHDELVARCAGYRRLVTAYARAAARRADAAAAAAA
                     GVEDAHRWPDLHPGDEVDDEVVVS"
     gene            1597..>3276
                     /locus_tag="VHM65_04945"
     CDS             1597..>3276
                     /locus_tag="VHM65_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2705085.1"
                     /translation="MSTSIATTSSLGAWATIRRGLQISPRLRQGLALTLVIALVSTAG
                     RVVIPVVVQRSIDDGILAVGGSRTEVVLRYGLVALLGLAVTAYSQFLVNVRLFEASEG
                     GLMQLRVAAFRHIHDLSVLTQNTERRGALVSRVTSDVDTISMFVQFGGLMLIIAVGQL
                     TLATVLMVAYSPPLAAVVWACYAPLILVIRVLQGIVGRAYTVVRQRVGEMLGAISEAV
                     VGAATIRAYGVADRTAQRIDAAVEAHRAAAMRAQVRAITSFTLGQGVSGLTTAAVLVV
                     GTVLAVNGRLTVGELLAFLFLVQLFTMPVQQGTEILNEMQNAVAGWRRVIGVIDTPAD
                     IADPGEAGVTLPRGPITVEVDDVEFAYPGGEPVLREVSLAFAPRTRVAIVGETGSGKS
                     TLAKLLTRLADPTRGVIRLDGADLRDISFASLRSRVVLVPQEGFLFDASLLENLTFGM
                     PDASPGRVGAVLRELGLGDWLDGLPAGLDTPVGQRGESLSAGERQLVALARAYLADPD
                     LLILDEATSAVDPHTEVTVQRAIDALTRGRTSIAIAHRLSTAEAADEVVVVD"
BASE COUNT          389 a         1233 c         1150 g          504 t
ORIGIN      
        1 gcgccgggtt gcggcagcac tgacgatgtt caacctcggc gcggacttcc gtcgccgggt
       61 gacccgccag tacctcacgc tgccgctgtc gtggcaccac cgccatccct cggggcagct
      121 gctctccaac gccaacgccg acgtcgaggc gatgtggcag gtcttcgcgc cgctcccgat
      181 ggcgctcggt gtcctcgtca tgctcgtcgt gggcgtggcc cagatggtgc tcgtcgaccc
      241 ctggctcgcg ctcatcgggc tcaccgtgtt cccgctgctg ttcgcggcca acagtgcctt
      301 ccagcgggcg atgtcaccgc gggtgacccg ggcccagcag ctgcgggccc aggtgtccga
      361 agtggcccac gagagcttcg aggccgcgct tgtcgtcaag tccctcggcc gcgagcagct
      421 cgagaccgac cggttcgcca cggtcagcga cgccctgcgt gcggcggccg tcgacgtcgg
      481 gcgcacccgc ggccggttcg accccgtgat cgaggcgatc ccgacgatcg gcaccctcgc
      541 cgtcctggtc gtcgggaccc agcgggttgt cacgggggcc ctgaccgccg ccgatgtcgt
      601 ccagatcgcc tacctgttct cgatcctcgc gttcccggtg cgggcgctcg gctgggtcct
      661 cggtgagctc cctcgctcgg tcgtggggtg gcgccgggtc tccgccgtgc tgcaggcccg
      721 aggcagcacc cggtatggcg cgcgcacgct ggcggggtcc ggcccggccg cgctgcgggc
      781 gcgcggggtc agctacgcct atgacgacgc gcccggccac gacgtgctgc gcgctgtgac
      841 gctcgacgtc ccggcgggcg gcaccgtcgc actcgtcgga ccgaccgggt cgggcaagtc
      901 gaccctggcc gacgtcctgc tgcgcctgct cgacgccgac cgcggcacgg tctggctcga
      961 cgggaccgat acccgcgagc tgactcgcga ggcgctgacc tgctcgaccg cgctcgtcgc
     1021 gcaggagacg ttcctcttcg acgacacggt cctgggcaac gtcaccctgg gggacgtcta
     1081 caccgaggac gaggtgtggg ccgcccttcg ggtggcccag gcggcggagt tcgtggccgc
     1141 cctgccgcag ggcctgcaga cccgcatcgg cgagcggggg acgagcctgt cgggcgggca
     1201 gcgtcagcga gttgccctgg cccgggcggt ggttcgccgg ccccgtgtcc tcgtgctcga
     1261 cgacgcgacg agcgcggtgg atccgagcgt cgagcaggcc atcctcgccc gagtcggtga
     1321 gagcggcgcc accgtgctgc tcatcgccta ccggatggcc acgatcgccc tggccgagga
     1381 cgtggtctat ctcgaggatg gagccgtgct cgaccacggc agccacgacg agctcgtcgc
     1441 gcgatgcgcc ggctaccgca ggctcgtcac tgcctacgcc cgcgccgccg cacgccgggc
     1501 cgacgcggcc gcagccgccg cagcgggcgt ggaggacgcc caccgctggc ccgacctgca
     1561 ccccggtgac gaggtcgacg acgaagtggt ggtctcgtga gcacgagcat cgcgacgacc
     1621 agcagcctcg gtgcctgggc cacgatccgc cgtggcctgc agatctcccc gcgcctgcgc
     1681 cagggcctgg cgctcaccct cgtcatcgcc ctcgtgtcga ccgcagggcg cgtcgtcatc
     1741 cccgtcgtcg tccagcggtc gatcgacgac ggcatcctcg ccgtcggcgg ctcgcgcacc
     1801 gaggtggtcc tgcgctacgg cctcgtcgcc ctgctggggc ttgccgtcac ggcatacagc
     1861 cagttcctcg tcaacgtcag gctcttcgag gcgtccgagg gcgggctgat gcagctgcgg
     1921 gtggcggcgt tccggcacat ccacgacctg tcggtgctga cgcagaacac cgagcggcgc
     1981 ggagccctgg tctcccgggt cacctcggac gtcgacacga tctcgatgtt cgtgcagttc
     2041 ggcggcctca tgctcatcat cgcggtcggc cagctcaccc tcgcgaccgt cctcatggtc
     2101 gcctacagcc cgccgctcgc ggccgtcgtc tgggcgtgct acgcaccgct gatcctcgtg
     2161 atccgggtgc tgcagggcat cgtcgggcgg gcctacacgg tcgtgcgcca gcgcgtcggc
     2221 gagatgctcg gggcgatctc cgaggccgtg gtcggggcgg cgacgatccg ggcctacggc
     2281 gtggccgacc ggaccgccca gcggatcgac gcggccgtcg aggcccaccg cgccgccgcg
     2341 atgcgggccc aggtgcgggc gatcacgtcg ttcacgctgg gccagggggt gtccggcctc
     2401 accacggccg ccgtcctcgt cgtcgggacg gtgctcgccg tcaacgggcg gctcaccgtg
     2461 ggggagctgc tcgccttcct cttcctcgtc cagctgttca cgatgccggt ccagcagggc
     2521 accgagatcc tcaacgagat gcagaacgcc gtcgccggct ggcgccgcgt catcggtgtc
     2581 atcgacaccc cagccgacat cgccgacccc ggtgaggcgg gcgtcaccct gccacgcggg
     2641 ccgatcaccg tcgaggtcga cgatgtcgag tttgcctatc ccggaggcga gcccgtgctt
     2701 cgggaggtga gcctcgcctt cgccccgcgg acccgggtgg ccatcgtggg cgagaccggg
     2761 tcgggcaagt cgaccctcgc caagctgctc acccggctgg ccgacccgac ccgtggggtc
     2821 atccgcctcg acggggccga cctgcgcgac atctccttcg cgtccctgcg cagccgggtc
     2881 gtcctcgtcc ctcaggaggg gttcctcttc gacgcctcgc tgctggagaa cctcaccttc
     2941 ggtatgccgg acgcctcgcc tggccgggtc ggagcggtcc tgcgtgagct cggcctcggc
     3001 gactggctcg acggcctgcc ggccgggctg gacacaccgg tcggtcagcg cggtgagtcg
     3061 ctgtcggccg gcgagcgcca gctcgtggcc ctggcccggg cctacctggc cgaccccgac
     3121 ctgctcatcc tcgacgaggc gacctccgcg gtggacccgc acacggaggt caccgtgcag
     3181 cgggccatcg acgccctgac ccgcgggcgc acctcgatcg ccattgccca ccgactctcg
     3241 acggcggagg ccgccgacga ggtcgtcgtc gtcgac
//
LOCUS       DATAIX010000334         1982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_126924, whole genome shotgun sequence.
ACCESSION   DATAIX010000334 DATAIX010000000
VERSION     DATAIX010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126924"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VHM65_04950"
     CDS             complement(<1..650)
                     /locus_tag="VHM65_04950"
                     /inference="COORDINATES: protein motif:HMM:NF025022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX2705086.1"
                     /translation="MAQTRSSTPASTAPRAHHPPIEPGQEVGVGLARRTFVLDTSVLL
                     SDPRAMLRFAEHEVVLPVVVITELEGKRHHPELGYFARQALRLLDELRLREGRLDHPV
                     PIGDAGGSLRVELNHTEPHALPAGFRLGDNDTRILAVAKNLANGGRAVTVVSKDLPMR
                     VKVAALGLAAEEYRAELAVDSGWTGMTELDVTTAEMDTLYDAGRLEHVGAAELPCHT"
     gene            complement(789..1550)
                     /locus_tag="VHM65_04955"
     CDS             complement(789..1550)
                     /locus_tag="VHM65_04955"
                     /inference="COORDINATES: protein motif:HMM:NF011403.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX2705087.1"
                     /translation="MRALDPLYGAYERLLARGIDPTATPRHVGVMLDGNRRWARANGF
                     DTASGHQAGANKIENLLEWCEEAGVEVVTLWLLSTDNLNRSQDEIGPLLKIIEGAVGD
                     LAARRRWRLNPIGALDLLPIETAESLKRSAELTRDVTGITVNVAVGYGGHREIADAVR
                     NLLLEHAAHGTPIEQIADHVTVEDIAAHLYTKGQPDPDLVIRTSGEQRLSGFLLWQSA
                     KSEFYFCEAYWPDFRHIDFLRALRSYASRERRFGS"
     gene            1789..>1982
                     /locus_tag="VHM65_04960"
     CDS             1789..>1982
                     /locus_tag="VHM65_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705088.1"
                     /translation="MTSAEPAQPRHLVEGEQRRPEGGEHPHIEAMVGAIRPHLRGWIH
                     LGTVPLTLAAGIVLVALAPPG"
BASE COUNT          283 a          687 c          709 g          303 t
ORIGIN      
        1 gtgtggcacg ggagctctgc cgcgccgaca tgctcgagcc ggccggcgtc atacagggtg
       61 tccatctcgg cggtggtgac gtcgagctcg gtcatcccgg tccagccgga gtcgacggcg
      121 agctcggcgc ggtactcctc ggcggccagc ccgagtgcgg ccaccttgac ccgcatcggc
      181 aggtccttgg agacgaccgt caccgcgcgg cccccgttgg cgaggttctt ggcgacggcg
      241 aggattcgcg tgtcgttgtc gccaaggcgg aagcccgcgg gaagggcgtg cggttcggtg
      301 tggttgagct cgacccgcag actgccaccg gcgtcaccga tcggtaccgg gtggtcgagc
      361 cggccctctc gaagccgcag ctcgtcgagc agcctcaggg cctggcgggc gaagtagccg
      421 agctcggggt gatggcgctt gccctcgagc tcggtgatga cgacgacggg cagcacgacc
      481 tcgtgctcag cgaagcggag catcgcacgc gggtccgaga gcagaaccga ggtgtcgagc
      541 acgaacgtcc gccgtgcgag ccccactccc acctcctgcc cgggctcgat cggcgggtgg
      601 tgtgcccgag gtgcggtcga agcgggggtc gacgagcggg tctgagccaa gtcgcactcc
      661 ttgcggcagt ggtcgcgttg cgcgcggtat tgacgacgct atgacgcagt atgacgtgcc
      721 gccgggacct gccgcggagt gttgcccagg tccgacgctc gactcgcggg caaccccgac
      781 ccacggactc agctgccgaa gcgccgctcc cggctcgcat acgagcgcag cgcccggagg
      841 aagtcgatgt gccggaagtc gggccagtat gcctcgcaga agtagaactc cgacttggcc
      901 gactgccaga gcaggaaacc gctgagccgc tgctcgcccg acgtgcggat caccaggtcg
      961 gggtccggct ggcccttggt gtacagatgg gccgcgatgt cctcgacggt gacgtggtcg
     1021 gcgatctgct cgatcggcgt gccgtgcgcg gcatgctcga gcagcaggtt gcgcaccgcg
     1081 tcggcgatct cacgatgacc gccatacccg acggcgacgt tgacggtgat cccggtgacg
     1141 tcacgcgtga gctcggccga tcgcttgagg ctctcggccg tctcgatcgg gagcaggtcg
     1201 agcgccccga tcgggttgag ccgccagcgg cgccgcgcgg cgaggtcgcc gacggccccc
     1261 tcgatgatct tgagcaacgg accgatctcg tcctgggagc ggttgaggtt gtcggtggag
     1321 agcagccaca gggtgacgac ctcgaccccg gcctcctcgc accactcgag caggttctcg
     1381 atcttgttgg cccccgcctg gtgcccgctc gccgtgtcga acccgttggc gcgagcccag
     1441 cgccggttgc cgtcgagcat gacgccgacg tgccggggag tcgccgtcgg gtcgatcccc
     1501 cgggcgagca gccgctcgta ggcgccatac agcgggtcga gcgcacgcac ccgaggctcc
     1561 ttcccgacgg tccgatatgt ccatacggta ctcgcccccg ccggtcgcac ccggtccgcc
     1621 cgttcgcgat ctcagccgcc ccggcagggg agttctggac cctgaggtgc cgccctcggg
     1681 gcgggccgat gaacggtgga tgaacggtcg gcgcccaggc cctgagcgac gctcggtgcg
     1741 caacctacgg gagcgtaacc tacggtcccg taggttagac ttgccggtat gacgtccgcc
     1801 gagccagccc agccccgtca cctcgtggag ggcgagcagc gccgacccga ggggggcgag
     1861 caccctcaca tcgaggccat ggtcggcgcg atcaggcccc acctgcgcgg ctggatccac
     1921 ctcggcaccg tccccttgac gctcgccgcc gggatcgtgc ttgtcgccct cgcgccgccg
     1981 gg
//
LOCUS       DATAIX010000335         4095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_246119, whole genome shotgun sequence.
ACCESSION   DATAIX010000335 DATAIX010000000
VERSION     DATAIX010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246119"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VHM65_04965"
     CDS             <1..230
                     /locus_tag="VHM65_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705089.1"
                     /translation="QILMSPTAKVPRTRFEARVVATALVGALVVAAQEWYAAGGAASI
                     LDLARASIDLVRTDRQPGRGDRPAQGPAQGP"
     gene            complement(344..1135)
                     /locus_tag="VHM65_04970"
     CDS             complement(344..1135)
                     /locus_tag="VHM65_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705090.1"
                     /translation="MVAISARGYRSLALRGDGTVVGWGLNENGQGTPPAGLTGVVAIS
                     LGFNHSLALRDDGTVVGWGHNSQGQATPPAGLTDAVAIAAGYNHSLALRSNGIVVGWG
                     FNGDGRATPPSGLTGVAAIAAGERHSLAVVQGHDSLALDVWAGPATKRTLVYSQGPAT
                     LTSGDYVIRPATGNVTSVTGTGTIPGLDGSRVTFDVTYNPATQKYAGAVDISGAGFDA
                     HIRVQAGRFGVTRTGDMVKGTLYGIKALSNPKRCFVIVFRIKDLG"
     gene            complement(1586..2116)
                     /locus_tag="VHM65_04975"
     CDS             complement(1586..2116)
                     /locus_tag="VHM65_04975"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX2705091.1"
                     /translation="MSLPHALLTSLIERPSSGLELARRFDRSIGYFWHATHQQIYREL
                     ARMESAGWVAADEVPGRGRKRVYRCLEAGRDELLRWAGKPTPATDPRDALLVRLRADA
                     ATGPLGLGEQIRDRMAHHEAALAAYRVIETRDFGPGAPSTRAARIQHLILAAGIVHEE
                     GGLRWCRQALDTLADP"
     gene            2249..>4095
                     /locus_tag="VHM65_04980"
     CDS             2249..>4095
                     /locus_tag="VHM65_04980"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HEX2705092.1"
                     /translation="MSPYPHLLEPLDLGFTTLPNRVIMGSMHVGLEEAPDGFERMAAF
                     YAERARGGVGLIVTGGIAPNDEGRPMPGGATLATAEEAARHTVVTAAVHAEGGRIALQ
                     ILHFGRYAYHPDLVAPSPLQAPISPFPPRELSAADIERTIEDFARCAALAQQAGYDGV
                     EIMGSEGYLLNEFLAARTNHRTDEWGGSYEHRMRFPVEIVRRVRERVGAEFIIIYRLS
                     MIDLVDEGSTLAEVITLAQAVEAAGATIINSGIGWHEARIPTIATSVPRAAFTWVTAR
                     LKGAVSIPLATSNRINTPEVAERVLADGQADLVSMARPFLADPDFVRKAAAGRADRIN
                     TCIGCNQACLDHTFGGLITSCLVNPRACHETELSIDPAPVARKVAVVGAGPAGMACAA
                     TAARRGHDVTLFEGADRLGGQFNLATQIPGKEEFRETLRYFATELADAGVATRLGTRA
                     GVADLVAGGFDVVVLATGVTPRRPDIEGVDHPKVVTYLQVLHDHVPVGRTVAVMGAGG
                     IGFDVAEYLTQDGGSATLDPAKFYAQWGIDASYTNRGGLTQPRHAPPAREVTLLQRKT
                     EKLGAGLGRTTGWIHRAELKNRGVQMVAGVTYDLIDDAGLHISVNGRQVV"
BASE COUNT          583 a         1446 c         1470 g          596 t
ORIGIN      
        1 cccagatcct catgtccccc actgcgaagg tcccgcgcac ccggttcgag gcccgcgtcg
       61 tggccaccgc tctcgtcggt gcgctcgtcg tcgcggcgca ggagtggtac gccgcgggag
      121 gagccgcgtc gatcctcgac ctcgcccggg cgagcatcga cctcgtccgc accgaccggc
      181 agcctggccg aggagacagg cccgctcagg gtccagctca gggtccgtga agaacatcac
      241 catctccgct caggatgggc gctgtgctcg tcgttgtgca gacggcgtca cggaccggtg
      301 gtcgtgtccg tcccgcaggc cgtcccgcag ggtcagccca gggtcagccc aggtccttga
      361 tccggaagac aatcacgaag cacctcttcg ggttgctcag ggctttgatc ccgtagagcg
      421 tgcccttgac catgtcgccg gtccgggtga cgccgaaccg gccggcctgc acgcggatgt
      481 gcgcgtcgaa cccggctcca gagatgtcga cggctcccgc gtacttctgc gtcgccgggt
      541 tgtaggtgac gtcgaaggtc accctggatc cgtccagccc tgggatcgtg ccggtgccgg
      601 tgaccgaggt gacgtttccg gtggccggcc tgatgacata gtcaccggag gtgagggtcg
      661 cggggccttg tgagtagacc aaggtccgct tcgttgctgg gccagcccag acgtcaaggg
      721 cgagggagtc gtggccttgc acgacagcca gactgtgtcg ttcgcctgcg gcgatcgccg
      781 cgacccccgt gagccctgac ggtggcgtcg ccctcccatc cccgttgaag ccccagccga
      841 cgacgatccc gttgctgcga agcgccagac tgtggttgta gccggcggcg atcgcaacag
      901 cgtcggtgag cccggccggg ggcgtggcct gtccttggct gttgtgcccc cagccgacga
      961 ccgtgccatc gtcacgcagc gccaggctgt ggttgaagcc gagggagatc gcgaccacgc
     1021 cggtgagccc cgcaggcggc gtcccctgtc cgttctcgtt gagaccccag ccgacgacgg
     1081 tcccgtcacc gcgcaacgcc aggcttcggt agccgcgcgc ggagatcgcg accacgtcag
     1141 tgagccccgg aggcggcgta gcctgcccta cgtattgata ggggcaacag aagccgttgt
     1201 agcccagccg acgacgtggt tcgccagcgg ctccgcgctg gctggcgccg ccggcgtggc
     1261 gaccagagcg gcagcgacga gcagtccgat ggccagcacc gaacgggcga gtgtcctggg
     1321 tcggggtcgg cgctgcgacg tgttcatggg tgactcctcc cagtcagcac ccggttccac
     1381 ggcgcaggct gggcggtgtg tcccgatgag gtcagaggct taggtccccg gcccccttgg
     1441 ccacgctgag gcacctcccg aggcgcagga ccgtctgccg gtgagcgtgc tcgggccgct
     1501 cgcgccatac gcggtcctcg tccacgaccg ccacaagtgg cgccgtcaga ccgctcgggg
     1561 tcggcgtcac gttccggcgc ggggttcaag ggtcggcgag ggtgtcgagc gcctggcggc
     1621 accaccgcag cccgccctcc tcgtgcacga tgcccgcagc gaggatgagg tgctggatgc
     1681 gcgcggcgcg ggtcgacggg gcgccggggc cgaagtcgcg ggtctcgatc acccggtatg
     1741 ccgcgagggc ggcttcgtgg tgtgccatcc ggtcgcggat ctgctcgccg aggccgagtg
     1801 gcccggtggc ggcgtcggcg cgcaggcgca cgagcagggc gtcgcgcggg tcggtcgccg
     1861 gggtcggctt gcccgcccag cggagcagct cgtcccgccc ggcctcgagg cagcggtaga
     1921 cccgcttgcg tccgcgcccg gggacctcgt cggcggcgac ccacccggcc gactccatcc
     1981 gggcgagctc gcggtagatc tgctggtgcg tcgcgtgcca gaagtagccg atcgagcggt
     2041 cgaaccgccg ggccagctcg agccccgagg agggccgctc gatgagcgag gtgagcagcg
     2101 cgtgcggcag cgacatacct cacagtctgc gcagcagtcg gcgtcaatgc aactggttgc
     2161 ataaacgtcg ggacgcgcct tagtgtggtc gcgtcggcgc acggcggccg gccactgtgg
     2221 tcaatgacga ggattggcag gcatctcgat gagtccctac ccgcacctgc tcgagccgct
     2281 cgacctcggc ttcacgaccc tgcccaaccg ggtcatcatg ggctcgatgc acgtcggcct
     2341 cgaggaggca ccggacggct tcgagcggat ggcggccttc tatgccgagc gggcccgcgg
     2401 gggtgtcggc ctcattgtca ccggtggcat cgcacccaac gacgagggtc gccccatgcc
     2461 cggcggagcg acgctggcga ccgcggagga ggcggcgcgc cataccgtgg tcacggcggc
     2521 cgtgcatgcc gaaggggggc ggatcgcctt gcagatcctg cacttcggcc gctacgccta
     2581 ccacccggac ctcgtcgccc cgagcccgct gcaggcgccg atctcgccct tcccgccgcg
     2641 cgagctcagc gccgccgaca tcgagcggac catcgaggac ttcgcccggt gcgcggcgct
     2701 ggcccagcag gccgggtacg acggcgtcga gatcatgggc tcggagggct acctgctcaa
     2761 cgagttcctc gccgcgcgga ccaaccaccg caccgacgag tggggcgggt cctacgagca
     2821 ccgcatgcgc ttccccgtcg agatcgtccg gcgcgtccgc gagcgggtcg gggccgagtt
     2881 catcatcatc taccgcctgt cgatgatcga cctcgtcgac gaagggtcca ccctggccga
     2941 ggtcatcact ctcgcccagg ccgtcgaggc cgccggcgcg acgatcatca actccggcat
     3001 cggctggcac gaggcccgga tcccgacgat tgcgacgagc gtgccgaggg cggccttcac
     3061 gtgggtgacc gcgcggctca agggtgcggt ctcgatcccg ctcgcgacgt ccaaccggat
     3121 caacaccccc gaggtcgccg agcgggtgct cgccgacggt caggccgacc tcgtctcgat
     3181 ggcgcggccg ttcctcgccg accccgactt cgtgcgcaag gcggcggcgg gccgggccga
     3241 ccggatcaac acgtgcatcg ggtgcaacca ggcctgtctc gaccacacct tcggggggct
     3301 catcacctcg tgcctggtca acccgcgcgc ctgccacgag accgagctga gcatcgaccc
     3361 ggctccggtg gcccgcaagg tcgccgtcgt gggcgcagga ccagccggta tggcgtgcgc
     3421 cgcgaccgcc gcccggcggg gccacgacgt gacgctcttc gagggtgcgg accgcctcgg
     3481 tgggcagttc aacctcgcga cgcagatccc gggcaaggag gagttccgcg agacgctgcg
     3541 ctacttcgcc accgagctcg ccgatgcggg ggtggccacg aggctcggga cccgggccgg
     3601 cgtggcggac ctcgtggccg gtgggttcga cgtcgtcgtg ctcgccaccg gggtcacgcc
     3661 gcgccggccc gatatcgagg gcgtcgacca ccccaaggtc gtcacctatc tgcaggtcct
     3721 gcacgaccac gtcccggtcg ggcggaccgt ggcggtcatg ggcgccggcg ggatcgggtt
     3781 cgacgtcgcc gagtacctca cccaggacgg cgggagcgcc acgctggacc cggcgaagtt
     3841 ctacgcgcag tgggggatcg acgcgtcata cacgaaccgg ggtggcctga cccagccccg
     3901 gcacgcaccc ccggcgcggg aggtgaccct gctgcagcgc aagaccgaaa agctcggcgc
     3961 ggggctgggg cgcacgaccg ggtggatcca ccgggccgag ctgaagaacc gcggcgtgca
     4021 gatggtggcg ggggtgacct acgacctgat cgacgacgcc gggctgcaca tcagcgtcaa
     4081 cggccgccag gtcgt
//
LOCUS       DATAIX010000336         6960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_47944, whole genome shotgun sequence.
ACCESSION   DATAIX010000336 DATAIX010000000
VERSION     DATAIX010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 6960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6960
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47944"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VHM65_04985"
     CDS             complement(<1..684)
                     /locus_tag="VHM65_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2705093.1"
                     /translation="MSQPTTAGAGVAGGSSPATADLLTLNNVEVIYDDVILVLRGLSL
                     RVPEGRIVALLGSNGAGKSTTLKAVSGLLPTEHGEVTDGTVTFDGTNVTHLDAAARVK
                     MGMSLCMEGRHVFEHLTVAENLVAGAYYRGGVDIGKDLETVYDLLPKLSDLSNSVAGY
                     LSGGEQQMLAIGRALMAKPRLLMLDEPSLGLAPLLVKEIFSFIKRINTEMGLTVLVIE
                     QNARRALAVA"
     gene            complement(684..1919)
                     /locus_tag="VHM65_04990"
     CDS             complement(684..1919)
                     /locus_tag="VHM65_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2705094.1"
                     /translation="MVFTTVFTTRKLRTRYGLVAAAAAVVLGVTACGGGSATSGDAGS
                     DKAPIKVGILADLTGATGDVGKPYNEGMLGYIDWVNAKGGISGRKIEPMSNDYAYKVP
                     TAEELYKKYVAAKAIVIQGWGTGDSEALRSKVAADKLPFMSASYAEALTDPAKSPYNF
                     VVAPTYSDQMRVALNWVAKDAGGKAEVAVFHNDSPFGTAPLADGQKWIQEKSLPLGFK
                     AYPMPKGTTSFTGLLSQAQAQGAKYIIIQNVSSPAAQVAKDVKANGLTMKIVCLNWCS
                     DELFIKTAGADNAEGTVMVQPFAPPTAAKPGHKTIEDFLKTKGATLADKGVHYAQGWY
                     TMDVMAKGIEKALAAGGDLTGESIRKALETMDAVDTGGVVGTGQVKFSAESHRGSTGS
                     GIYEVKAGAFVEIAANQTP"
     gene            complement(1991..3049)
                     /locus_tag="VHM65_04995"
     CDS             complement(1991..3049)
                     /locus_tag="VHM65_04995"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX2705095.1"
                     /translation="MATATGIHHRTYRAELALRHTKAEHVRLGLVVVGLIATPFLLDS
                     YWLGIANTILIAVVGAVGLNILVGYTGQISLGQGGFLAVGSYTSAIVSTRAGLPVPVS
                     VVIAVVMTAAVGALFGLPALRLKGLYLAIATLASQEIILFVVRRWEFLTEGKGYIEVP
                     RLTVFGLQIERDTFEFQWYWILAVIATLSVVAGRNLFRTALGRSFMAVRDQDIAAQAI
                     GVNLTRAKVTAFAVSSGFVGLAGALTAYYTETVTWERYTLDVSVLYLAMIIVGGLGSI
                     AGSVYGAVFMMLLPALLTEAADKLAASLPVLSEQLPAIKNGVFGLAIVVFLLVEPRGL
                     DRIWSRIKDYVRFWPFRY"
     gene            complement(3052..3924)
                     /locus_tag="VHM65_05000"
     CDS             complement(3052..3924)
                     /locus_tag="VHM65_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX2705096.1"
                     /translation="MSTFISLSIYGLADGAILALAALGFVLIYKATRVINFAQGEFLL
                     VGAYTFYAAFVVMRLPLVLASLVGVGFAIGLGVVVERFVLRPLVGESAISVIMVTIGL
                     SAVLKAVVQLFFGTSVREQPKILPTGRITLAGATVPLNRILVIVIAAAVLAAFTVFFR
                     RSRHGIAMRAVADDQQAALTMGISVRRIFALAWALAAVSALIAGVLLADLSAVEQNIA
                     SFGLIVFPVVILGGLDSVPGTVVGGLTIGLLKWYVSGYADPGLAEVVPYLVLVAILMF
                     RPYGIFGETRIERV"
     gene            complement(3921..5897)
                     /locus_tag="VHM65_05005"
     CDS             complement(3921..5897)
                     /locus_tag="VHM65_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2705097.1"
                     /translation="MAHDTFPRLLAKLASERPDQTALQEKRYGIWQPLSWSGYAGRVR
                     DFAHGLAALGVERGQVVAVLGDNRPEWLVAELATQSIGAAVVGIYPTSIGEELLHILT
                     LARVRIVVAEDQEQVDKLIRLREEQAATPTPDMPPLLVETVVFYDPHGLEQYSHDYLR
                     DFNDIEALGREWGEAHPGWLDEQVALGAASEIAVICTTSGTTSRPKLAELTHANLLSM
                     AEHLTTIDPIAAKDRYVSFLPFAWIGEQMLAVACGLSQGLTLSFPEDSATQRADLREI
                     GPNVMFSPPRIWESMLSEVQVRIDEAGRLKRAVFGWGYAIGDKVAGRQIRGQSIGALT
                     ILHAIADQVALRPVRDQLGLARIKHCYTGGAPLGPDVFRFFHAIGVNLKQIYGQTEIC
                     GIAVVHRDDDIEFHTVGTPIPSTEILIADNGEILLRSASVFRGYHRNPAATAEAVDTV
                     GWLHTGDAGYLEDNGHLVVIDRQKDVLTTADGTRFSTAFIENKLKFSPYVEEAVVFAT
                     DTDGMTALITIDPLTTGSWAEHERLSYTTYTDLAGKAEVRELLAEETNRANEDLPASI
                     RVKRFLLLHKQFDPDDDEITRTRKVRRNVIEARYALIIEALARGAENVEIRSSVTYQD
                     GSKVERSIDLVIVDMSTYREPEGRGRRPVWSGRR"
     gene            complement(5897..6736)
                     /locus_tag="VHM65_05010"
     CDS             complement(5897..6736)
                     /locus_tag="VHM65_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2705098.1"
                     /translation="MLTRAYLHELNSSRVPTGQPVLEISDITLRFGGVLALADVGLTV
                     TQGHIHAIIGPNGAGKSSLLNCVSGLYHPQRGTIVLRTGDRSTTAELTRLPPHKIARL
                     GVARSFQNIELFTHLTVLENLMLGRHVHMSQSVAASLLWFGPARRQEIAHRALTEEVI
                     DLLSLQAHRHQPVGSLAYGIQKRVELGRALCLQPALLLLDEPMAGMNAEEKEDMARYV
                     LDVHELAGVTVILIEHDMAIVMDISDRVSVLDFGRLIADGTPDEVKADPAVVEAYLGA
                     EED"
BASE COUNT         1106 a         2212 c         2516 g         1126 t
ORIGIN      
        1 ggccaccgcg agggcgcggc gagcgttctg ctcgatgacg agaacggtca ggcccatctc
       61 ggtgttgatc cgcttgatga aggagaagat ctccttgacg aggagcggag ccagccccag
      121 tgacggctca tcgagcatga gcagccgggg cttggccatg agtgcccgtc cgatggcaag
      181 catctgctgc tcgcccccgg acaggtagcc ggccacgctg ttgctcaggt cgctgagctt
      241 gggcagcagg tcgtagaccg tctcgaggtc cttgccgatg tcgacgcccc cgcggtagta
      301 cgctcccgcg acgaggttct cggccaccgt gaggtgctcg aagacgtgcc gtccctccat
      361 gcacaggctc atacccatct tgacccgggc ggccgcgtcg aggtgggtga cgttcgtgcc
      421 gtcgaaggtc accgtcccgt cagtgacctc gccgtgctcg gtcggcagca gcccggagac
      481 tgccttgagc gttgtggact tgcccgcgcc gttggacccg agcagggcca cgatccggcc
      541 ctcgggcacc cgcagcgaca ggccgcgcag cacgaggatc acgtcgtcgt agatgacctc
      601 gacgttgttg agggtcagca ggtcggcggt ggccggcgac gagccgccgg ccacccccgc
      661 gcctgccgtg gtgggctggc tcattacgga gtctggttcg ccgcgatctc gacgaacgcg
      721 cccgccttga cctcgtagat gcccgagccg gtggagccac ggtgcgattc cgcgctgaac
      781 ttcacctggc cggtgccgac gacgccgccc gtgtcgacgg cgtccatcgt ctccagcgcc
      841 ttgcggatgg actcgccggt gaggtcgccg ccggcggcca gtgccttctc gatgcccttg
      901 gccatgacgt ccatcgtgta ccagccctgg gcgtagtgga cgcccttgtc ggccagcgtg
      961 gcgcccttgg tcttgaggaa gtcctcgatg gtcttgtggc ccggcttggc ggccgtgggc
     1021 ggcgcgaacg gctgcaccat gacggttccc tcggcgttgt cggcgccggc ggtcttgatg
     1081 aacagctcat ccgagcacca gttgagacag acgatcttca tggtcaggcc gttggccttg
     1141 acgtccttgg cgacctgggc ggccgggctc gagacattct ggatgatgat gtacttcgcg
     1201 ccctgagcct gcgcctgaga gagcagcccg gtaaagctcg tcgtcccctt gggcatcggg
     1261 taggccttga agccgagcgg caggctcttc tcctggatcc acttctggcc gtcggccagc
     1321 ggggcggtgc cgaacgggct gtcgttgtgg aagaccgcca cctcggcctt gccgccggcg
     1381 tccttggcga cccagttcag cgcgacgcgc atctggtcgg agtaggtcgg ggcgacgacg
     1441 aagttgtatg gcgacttcgc ggggtcggtg agggcctcgg cgtacgacgc ggacatgaac
     1501 ggcagcttgt ccgcggcgac cttggaccgc agtgcctcgg agtcgcccgt gccccagccc
     1561 tggatgacga tggccttggc ggcgacgtac ttcttgtaga gttcctcggc ggtcgggacc
     1621 ttgtaggcgt agtcgttgga cattggctcg atcttgcggc cgctgatccc gcccttggcg
     1681 ttgacccagt cgatgtatcc gagcatgcct tcgttgtagg gcttgcccac gtcaccggtc
     1741 gcgccggtga ggtcggcgag gatgccgacc ttgatgggtg ccttgtcgct gcctgcgtcg
     1801 ccgctcgtcg ccgagccgcc accgcatgcg gtgacgccga ggacgacggc cgcggcggcc
     1861 gcgacaagtc cgtaccttgt cctgagcttc ctggtcgtga ataccgtcgt gaataccatg
     1921 gtgtgactcc attcctgggg gccgggggcc ggcctgggtt tcagcgtggg ctaactgttt
     1981 gggctgtcgg tcagtagcgg aacggccaga accggacgta gtccttgatg cgggaccaga
     2041 tccggtcgag gccgcggggt tcgacgagga ggaacacgac gatggcaagg ccgaagacgc
     2101 cgttcttgat cgccggaagc tgttcggaca ggaccggcag ggaggcggcg agcttgtcgg
     2161 cggcctcggt gagcagcgcg ggcagcagca tcatgaagac cgcaccgtag accgagccgg
     2221 cgatgctgcc gaggcctccc acgatgatca tagcgaggta gagcaccgag acgtcgaggg
     2281 tgtagcgctc ccaggtgacc gtctcggtgt agtaggcggt gagcgccccc gccagcccga
     2341 cgaacccgct ggacacggcg aacgccgtga ccttggcccg ggtgaggttg acgccaatgg
     2401 cctgggcggc gatgtcctgg tcccggacgg ccatgaacga gcgcccgagg gcggtgcgga
     2461 acaggttccg gccggccacg acgctcaggg tcgcgatcac cgcgaggatc cagtaccact
     2521 ggaactcgaa ggtgtcccgc tcgatctgca ggccgaacac ggtgagccgc gggacctcga
     2581 tgtagccctt gccctcggtg aggaactccc accgacggac cacgaacaag atgatctctt
     2641 gcgaggcgag cgtcgcgatg gccaggtaga gccccttgag ccgcaacgcc gggaggccga
     2701 acaaggcgcc gactgcggcc gtcatgacga cggcgatgac gacgctgacc ggcacgggca
     2761 gccccgcacg ggtcgacacg atcgccgacg tgtacgaccc gaccgcgagg aagccgccct
     2821 ggccgaggga gatctggccg gtgtagccga cgaggatgtt cagacccacg gccccgacca
     2881 cggcgatgag gatcgtgttg gcgatcccga gccagtagga gtcgagcagg aagggtgtcg
     2941 cgatgagccc cacgacgacg agcccgagcc ggacgtgctc ggccttggta tggcgaagcg
     3001 ccagctcggc tcggtaggtc cggtgatgga tgccggttgc ggtggccatg gtcagaccct
     3061 ctcgatccgt gtctcgccga agatgccgta gggccggaac atgaggatcg cgacgagcac
     3121 gagatacggg acgacctcgg ccaggccggg atcggcatag cccgagacgt accacttgag
     3181 cagaccgatc gtcaggccgc cgacgacggt gcccggcacc gagtcgaggc ccccgaggat
     3241 gacgaccggg aagacgatga gcccgaagga cgcgatgttc tgctccacgg ccgacaggtc
     3301 cgcgagcagg acgccggcga tgagtgcgct cacggccgcc agggcccagg cgagggcgaa
     3361 gatgcggcgc accgagatgc ccatcgtcag cgcggcctgc tggtcgtcgg ccacggcccg
     3421 catcgcgatg ccgtggcggg atcggcggaa gaacacggtg aaggcagcga gcacggccgc
     3481 ggcgatgacg atgacgagga tgcggttgag ggggaccgtg gcgccggcca gggtgatcct
     3541 cccggtcggc aggatcttcg gctgctcgcg caccgaggta ccgaagaaca gctggacgac
     3601 cgccttgaga acggcagaca ggccgatcgt caccatgatg acgctgatcg cgctctcccc
     3661 gacgagcggt cgcagcacga agcgctcgac gacgacaccg aggccgatgg cgaagccgac
     3721 tcccacgagg gacgcgagga ccagcggcag gcgcatgaca acgaaggcgg cgtagaacgt
     3781 gtaggcgccg acgagaagga actcaccctg ggcgaagttg atgacccggg ttgccttgta
     3841 gatgaggacg aagccgagcg cggccagggc caggatcgcc ccatcggcga gcccgtagat
     3901 cgacagggag atgaaggtgc tcatcgacgt ccgctccaga ccggtcggcg gccccggccc
     3961 tcgggctcgc ggtaagtgga catgtcgacg atcaccaggt cgatgctccg ctcgaccttg
     4021 gacccgtcct ggtaggtcac cgagctgcgg atctcgacgt tctcggcgcc gcgtgccaat
     4081 gcctcgatga tcagggcata gcgcgcctcg atgacgttgc gccgcacctt gcgggtgcgg
     4141 gtgatctcgt cgtcgtccgg gtcgaactgc ttgtgcagca ggaggaagcg cttcacccgg
     4201 atactcgccg gcaggtcctc gttggcccgg ttcgtctcct cggcgagcag ctcgcgcacc
     4261 tccgccttgc cggccaggtc cgtgtatgtc gtgtacgaca ggcgctcgtg ctcggcccag
     4321 gagcccgtgg tgagggggtc gatcgtgatg agggccgtca tcccgtcggt gtcggtcgca
     4381 aagacgacag cctcctcgac atacggggag aacttgagct tgttctcgat gaacgcggtg
     4441 gagaagcggg tgccgtcagc cgtggtgagg acgtccttct gccggtcgat gacgacgagg
     4501 tggccgttgt cctccaggta gccggcgtcg ccggtgtgca gccagcccac cgtgtcgacc
     4561 gcctcggccg tggcggccgg gttgcggtgg tagccccgga agaccgacgc cgagcgcagc
     4621 aggatctcgc cgttgtcggc gatgaggatc tccgtcgacg ggatcggcgt gccgaccgtg
     4681 tggaactcga tgtcgtcgtc gcggtggacg acggcgatcc cgcagatctc ggtctggccg
     4741 tagatctgct tgaggttgac accgatcgcg tggaagaagc ggaagacgtc gggcccgagg
     4801 ggcgcgccac cggtgtagca gtgcttgatc cgggccaggc cgagctggtc ccgcaccggc
     4861 cgaagggcga cctggtcggc gatggcgtgc aggatcgtca gcgcgccgat gctctgcccg
     4921 cggatctggc gcccggcgac cttgtctccg atggcatagc cccagccgaa gacggcgcgc
     4981 ttgagccggc cggcctcgtc gatgcggacc tggacctcgg agagcatcga ctcccagatc
     5041 cggggcgggg agaacatgac attggggccg atctcgcgca gatcggccct ctgggtcgcc
     5101 gagtcctcgg gaaaggacag ggtgaggccc tgagacaggc cacaggcgac ggcgagcatc
     5161 tgctcgccga tccaggcgaa cggcaggaag gagacatagc ggtccttcgc ggcgatcggg
     5221 tcgatcgtcg tgaggtgctc cgccatcgag agcaggttgg cgtgggtgag ctcggcgagc
     5281 ttgggccggc tcgtcgtgcc ggaggtcgtg cagatgaccg cgatctcgct cgcggcgccg
     5341 agggcgacct gctcgtcgag ccagccaggg tgggcctcac cccactcgcg gcccagggcc
     5401 tcgatgtcgt tgaagtcccg caggtagtcg tggctgtact gctccagccc atgcgggtcg
     5461 tagaagacga cggtctcgac cagcaggggc ggcatgtccg gggtcggcgt ggcggcctgc
     5521 tcctcgcgca gccggatcag cttgtcgacc tgctcctggt cctcggccac gacgatgcgc
     5581 acgcgggcca gggtgaggat gtgcaacagc tcctcgccga tgctcgtcgg gtagatcccg
     5641 acgaccgcgg cgccgatgct ctgggtggcc agctcggcga cgagccactc cggccggttg
     5701 tcgccgagga cggccacgac ctgccctcgc tcgacaccga gtgcggccag accgtgcgcg
     5761 aagtcccgca cgcgcccggc atacccgctc caggacagcg gctgccagat cccgtagcgc
     5821 ttctcctgca aggcggtctg gtccggccgc tcgctcgcga gcttggcgag caggcgcggg
     5881 aaggtgtcgt gggccatcag tcctcctcgg ccccgaggta ggcctcgacc acggcagggt
     5941 cggccttgac ctcgtcgggc gtcccgtcgg cgatgaggcg accgaagtcc aggacgctga
     6001 cccggtctga gatgtccatg acgatggcca tgtcgtgctc gatgaggatg acggtgaccc
     6061 cggcgagctc gtggacgtcg aggacgtagc gggccatgtc ctccttctcc tcggcgttca
     6121 tcccggccat cggctcgtcg agcaggagca gggcgggctg caggcacagg gcccggccga
     6181 gctcgacccg cttctggatg ccgtaggcca gcgaccccac cggctggtgg cggtgggcct
     6241 gcagcgagag caggtcgatg acctcctcgg tgagcgcgcg atgggcgatc tcctggcgcc
     6301 gggcggggcc gaaccacagc agcgatgccg ccacgctctg gctcatgtgg acatgccggc
     6361 cgagcatgag gttctcgagg acggtgaggt gggtgaacag ctcgatgttc tggaaggacc
     6421 gggccacccc gagccgggcg atcttgtgcg gggggagccg ggtgagctcg gcggtcgtgc
     6481 tccggtcgcc ggtgcgcagc acgatcgtgc ctcgctgcgg gtggtagagc ccgctgacgc
     6541 agttgagcag gctggacttg cccgcgccgt tgggaccgat gatcgcgtgg atgtgcccct
     6601 gggtgacggt cagcccgaca tcggcgaggg ccagcacccc tccgaagcgc agggtgatgt
     6661 ccgagatctc caggacgggc tggccggtgg ggacgcgcga ggagttcagc tcgtgcaggt
     6721 aggcacgggt cagcactcgg gacctccttt ggtcgagcca ctcatcatgc cgactaccca
     6781 cccgtccgac tactgtgatc ataggtttcc actatgtgga aacgcaatgc ttgttgtgaa
     6841 actggaatcc gggacgtaga gtcgtcgaca cggtaggagc gagggggttc cggtcggtcg
     6901 cgggactcgc ctggtatgac ggcggcgcgc gacgctggtc cgcaggaagg tgaggcgccc
//
LOCUS       DATAIX010000337         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_167449, whole genome shotgun sequence.
ACCESSION   DATAIX010000337 DATAIX010000000
VERSION     DATAIX010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167449"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VHM65_05015"
     CDS             <1..750
                     /locus_tag="VHM65_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX2705099.1"
                     /translation="AHCDPSLDTGEVGLLVGPLTAAADAIEVTLTGRGGHTSRPHLTQ
                     DLTFALAKVITDVPAALSRRLDPRAGAVLVWGHVESGVAPNVIPSTGTASGTLRMLDA
                     EAWAGVGPLVEELVTAVVSPYGVQAETRYVPGVPPVVNERASVDALREGVAFAGSDAV
                     PTSQSLGGEDFAWYLTLVPGAMARLGTRPLGGPTYDLHQGDLVVDEAAVILGAKVLVG
                     AAIASIPQLDAERAAPPNGSEDDNGSEDDNR"
     gene            747..>1503
                     /locus_tag="VHM65_05020"
     CDS             747..>1503
                     /locus_tag="VHM65_05020"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF006847.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX2705100.1"
                     /translation="MNETIAAIPKVLLHDHLDGGLRPETIIELASEQGYDALPESEPD
                     ALGAWFRDSADSGSLVRYLETFSHTVGVMQTREALIRVASECVQDLAADGVVYVESRY
                     APELHIAAGLALSDVVEAVNAGLRQGERLAAEAGHRIQARALLTAMRHAAKSSEIAEL
                     VVRYRDAGVGGFDIAGAEAGYPPTRHLDAFEYLSRENAHFTIHAGEAFGLPSIWEALQ
                     WCGADRLGHGVRIVDDITIDGLPFGEWVTSGVTT"
BASE COUNT          212 a          548 c          527 g          216 t
ORIGIN      
        1 gcccactgtg acccctcgct cgacaccggc gaggtcgggc tgttggtcgg tccgctcacg
       61 gcggccgccg acgccatcga ggtcacgctc acgggccgcg gcgggcacac ctcacggccg
      121 catctcaccc aggacctcac cttcgccctc gccaaggtca tcaccgacgt gcccgcggcc
      181 ctgtcgcgcc gcctcgaccc gcgcgctggt gcggtcctcg tgtgggggca tgtcgagtcc
      241 ggcgtcgctc ccaacgtcat cccgtcgacg ggcacggcca gcggcacctt gcggatgctc
      301 gacgccgagg cgtgggccgg ggtgggcccg ctcgtcgagg agctcgtcac cgcggtcgtc
      361 tccccgtacg gcgtgcaggc cgagacccgc tatgtgcccg gggtgccccc ggtggtcaat
      421 gagcgcgcgt cggtcgacgc cctgcgcgag ggtgtcgcct tcgccgggag cgacgcggtg
      481 ccgaccagcc agtctctcgg tggtgaggac ttcgcgtggt acctcacgct ggtgcccggc
      541 gcgatggccc ggctcgggac ccggcccctc ggcggcccga cctacgacct gcaccagggg
      601 gacctcgtcg tcgacgaggc ggccgtcatc ctcggcgcca aggtgctcgt cggcgcggcc
      661 atcgcgagta tcccccagct cgacgccgaa cgcgccgcgc cgcccaatgg ctccgaggac
      721 gacaatggct ccgaggacga caacagatga acgagaccat cgcagcgatc cccaaggtcc
      781 tgctgcacga ccacctcgac ggcgggctac gcccggagac gatcatcgag ctggcgagcg
      841 agcaggggta tgacgccctg ccggagagcg agcccgacgc cctgggtgcg tggttccggg
      901 acagtgccga ctccgggtcg ctcgtgcgat acctcgagac gttcagccac accgtcgggg
      961 tcatgcagac ccgtgaggcg ctcatccggg tcgcgagcga gtgtgtccag gacctcgccg
     1021 ccgacggcgt cgtctacgtc gaaagtcgtt acgccccaga gctgcacatt gctgctgggc
     1081 tagccctgag cgacgtcgtg gaggccgtca acgcgggtct gcgccagggc gagcggctcg
     1141 ccgccgaggc gggtcaccgg atccaggcgc gggcgctgct cacggcgatg cgccacgcgg
     1201 ccaagagcag cgagatcgcc gagctcgtcg tccgctaccg cgacgcgggc gtcggcggtt
     1261 tcgacatcgc cggggccgag gcgggctacc caccgacgcg tcatctcgac gcgttcgagt
     1321 acctcagtcg cgagaacgcc cacttcacca tccacgcggg tgaggcgttc gggctcccca
     1381 gcatctggga ggcattgcag tggtgcggcg ccgaccggct cggccatggt gtgcggatcg
     1441 tcgacgacat cacgatcgac gggctcccgt tcggggagtg ggtcacatcg ggcgtcacca
     1501 ccc
//
LOCUS       DATAIX010000338         3606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_186759, whole genome shotgun sequence.
ACCESSION   DATAIX010000338 DATAIX010000000
VERSION     DATAIX010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186759"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VHM65_05025"
     CDS             complement(<1..407)
                     /locus_tag="VHM65_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705101.1"
                     /translation="MSRPTTEGEYLARLIPESVRAQQLSRRTMLRGAAGAGALLMTPS
                     LLAACGGSSTSSSTGSAGGAATGVVSFGSNYSDKVPKAAMAAVLDAFAKAKPGLSTKI
                     NTIDHNTFQENINTYLQGAPDDVFTWFAGFRMR"
     gene            737..2740
                     /locus_tag="VHM65_05030"
     CDS             737..2740
                     /locus_tag="VHM65_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX2705102.1"
                     /translation="MRPWPSDVLPFGGDYNPEQWPEPTWPGDIDLMRRAGVTFVSLGI
                     FSWSWLEPADGQYDFGWLDRVMGLLADAGIAVDLATATASPPMWLSAKHPGLGPVDHD
                     GRRLWPGSRQTWCPSSATFTSYATRLAQAMAERYHDHPALAMWHVSNEYGCHHTPCYC
                     ETCAAGFRGWLQGRYGELARLNDAWGTAFWSQRYSAWEQVIPPRLTPAIANPGQVLDY
                     RRYQSDALLRQYLAERDVLREHSPGVPITTNFMTMRHFTHLDYHRWAGQMDLDHEVVS
                     TDHYVVDALEDPWAEQAFCGDLTRGIAGGRPWVLMEHSASAVNWQPVNRAKPPGQMQR
                     DSLTHVAHGADALGFFQWRASRAGAEKFHSGLVPHAGADSRVFREVCELGAVAGRLGE
                     VVGSRVAADVAILWDYEAGWGVSGPALPSTLVSYPDQAIAIHRALRERHVSADVVHPG
                     ADLSRYRLIVVPTLYLVSDATASALESAAAAGAHVLVTYFSGIVDERDHIRLGGYPGA
                     FRHLLGIRVSEFLPLRAGETVALRDGSQATVWSEDLTPEGATVVTAYGEGPLTGQPAV
                     TRYDVAGTSRWYVSTSLDRIALGALVGAAIDSAGVRPVLAGLPHGVDATRRAGGAGSW
                     VFLVNTTEAALDVAVDGYDLVADRAVTGSLRLGAGATAVIREG"
     gene            2744..3574
                     /locus_tag="VHM65_05035"
     CDS             2744..3574
                     /locus_tag="VHM65_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX2705103.1"
                     /translation="MLASQRRDVILGHVQAGGARVSALVELLGVSDMTIRRDIEHLVT
                     QGLVHKVHGGALPTGAMSAVEPGFIIKSELQVAHKQAIARAAAALVKPGSAIGISAGT
                     TTYQVARELRAVPNLTIVTNSMLIAQVLQDGVEIGQTVILTGGTRTPSDALVGPVAVD
                     ALHGLHVDLLFLGVHGMTIQTGLTTPNIVEAETNRALVAAATRTVVTADHSKWGVIGL
                     ASFGRLELVERLVTDSGLPGPAREAIEALGCRVDLVDLLEDPARLDGAGSRSRPSHHG
                     "
BASE COUNT          527 a         1310 c         1222 g          547 t
ORIGIN      
        1 aaccgcatcc ggaagccggc gaaccaggtg aagacgtcgt ccggagctcc ctggaggtag
       61 gtgttgatgt tctcctggaa ggtattgtgg tcgatcgtgt tgatcttggt ggacagtccc
      121 ggtttggcct tggcgaacgc atccaacacc gccgccatgg cggccttggg caccttgtcc
      181 gagtagttcg acccgaagga cactaccccc gtcgcggcgc ctccggcgct tccggtggac
      241 gagctggtcg agctgccgcc gcaggccgcc agcaggctgg gggtcatgag cagggctcca
      301 gcgcccgcgg caccgcgcag catcgtgcgc cgggacagct gctgggcgcg gacggactcg
      361 gggatcaggc gggcgaggta ctcgccttcg gtggtggggc gggacatgac gtctcctttg
      421 acgcgactca acaagcgaca ctcaacaagg acactcaaca acgactctgc accaggggtg
      481 cagcgtcact cctcacttcc gcgatgcaaa accaacagag ttcaacaccc gtgatgagga
      541 tgactagatc gtcttcccct cgactcccgt tgtcaaccct tccacgccgg gtgagtccaa
      601 acagtcacca agcactactt gggcgagcga gccgcccgca gcggcgcatc gagcccacgc
      661 tggtccttcg ccggagtcag gtcaagacat gttgagtttt gttgacaccc gtcctggaca
      721 ggtcgagact ggctccatgc gcccatggcc cagtgacgtc ctgccgttcg gtggcgacta
      781 caacccagag cagtggccgg agccgacctg gcccggggac atcgacctca tgcgacgcgc
      841 cggggtgacc ttcgtcagcc tcgggatctt ctcctggtcc tggctggagc ccgccgacgg
      901 ccagtacgac ttcgggtggc tcgaccgggt catgggcctg ctcgccgacg cagggatcgc
      961 ggtcgacctg gccaccgcga ccgcttcgcc tccgatgtgg ctcagcgcca agcatcccgg
     1021 gctgggcccc gtcgaccacg acggccgacg cctctggccc ggttcgcgcc agacctggtg
     1081 ccccagctcg gcgaccttca cgtcatacgc gacccgcctg gcacaggcga tggccgagcg
     1141 ttaccacgac cacccggccc tggcgatgtg gcacgtctcc aacgagtacg gctgccacca
     1201 cacgccgtgc tactgcgaga cctgcgcggc cggcttccgc gggtggctcc agggccggta
     1261 tggcgagctg gcgcggctca acgacgcgtg ggggacggcg ttctggagcc agcgctactc
     1321 ggcgtgggag caggtcatcc cgcctcggct cacgccggcc atcgccaacc ccggccaggt
     1381 cctggactac aggcgctacc agtccgacgc gctcctgcgt cagtacctcg ccgagcgtga
     1441 cgtcctgcgg gaacactccc ccggcgtccc gatcacgacg aacttcatga cgatgcgcca
     1501 cttcacccac ctggactacc accgctgggc cggccagatg gacctcgacc acgaggtggt
     1561 ctccaccgac cactacgtcg tggacgcgct cgaggacccc tgggccgagc aggccttctg
     1621 cggggacctg acccgcggta tcgccggagg ccggccctgg gtgctcatgg agcactcggc
     1681 gagcgccgtg aactggcagc cggtcaaccg ggccaagccc ccgggccaga tgcagcgcga
     1741 cagcctgacc catgtggcgc atggggctga cgcgctgggg ttcttccagt ggcgcgcgtc
     1801 gcgggccggt gccgagaagt tccactccgg cctggtcccc catgccgggg cggactcgcg
     1861 ggtcttccgc gaggtgtgcg agctcggtgc ggtggcggga cggctcggcg aggtcgtcgg
     1921 gagccgggtc gcggccgacg tggcgatcct gtgggactac gaagccggct ggggcgtgtc
     1981 cggaccggcc ctgccctcca ccctcgtgtc ctaccccgac caggccatcg ccatccaccg
     2041 cgcactgcgc gaacggcacg tcagcgccga tgtcgtccac cccggcgccg acctgtcgcg
     2101 ctaccggctc atcgtcgtcc cgacgctcta cctggtctcg gacgcgacgg cctccgccct
     2161 cgagtccgca gctgccgcag gcgcgcacgt cctcgtgacg tacttctccg gcatcgtcga
     2221 cgaacgggac cacatccgcc tcggcggcta ccccggcgcc ttccgccacc tgctcggcat
     2281 ccgggtgagc gagttcctgc cactgcgcgc cggggagacc gtggcgcttc gcgacgggtc
     2341 ccaggcgacg gtgtggtccg aggacctgac ccccgagggc gcgacggtcg tgacggcata
     2401 cggcgagggt ccgctcaccg ggcagcccgc agtgacccgg tatgacgtgg ccggcacctc
     2461 gcgctggtac gtgagtacct ccctcgaccg gatcgcactc ggcgcgctcg tcggggccgc
     2521 catcgactcg gccggggtcc ggcccgtgct ggccggcctg ccgcacggtg tcgacgccac
     2581 ccggcgggcc gggggtgccg ggtcctgggt gttcctcgtc aacacgaccg aggcggcgct
     2641 cgacgtggca gtcgacggct acgacctggt ggccgaccgc gccgtcaccg ggtcgttgcg
     2701 gctcggggcg ggtgcaaccg cggtcatccg cgagggctga cccgtgctcg ccagccagcg
     2761 ccgcgacgtg atcctcggtc acgtccaggc cggcggcgcg cgcgtctcgg cactcgtcga
     2821 gctgctcggt gtgtcggaca tgacgatccg gcgcgacatc gagcacctgg tcacgcaggg
     2881 gctggtccac aaggtgcacg gcggagccct tcccaccggg gcgatgagcg ccgtcgagcc
     2941 ggggttcatc atcaagtccg agcttcaggt ggcgcacaag caggccatcg cccgggcggc
     3001 cgcagccctg gtcaagccgg gctcggcgat cgggatctcg gccgggacga cgacctacca
     3061 ggtggcccgc gagctgcgcg ccgtccccaa cctcaccatc gtgacgaact cgatgctcat
     3121 cgcgcaggtg ctgcaggacg gcgtcgagat cgggcagacc gtgatcctca ccggcggcac
     3181 ccgcaccccc tcggatgcgc tcgtcgggcc cgtcgcggtc gacgcgctgc acggcctgca
     3241 tgtcgacctg ctcttcctcg gcgtccacgg gatgacgatc cagaccggcc tgacgactcc
     3301 caatatcgtt gaagcagaga ccaaccgggc acttgtcgcc gcggccacac gcacggtggt
     3361 gaccgcggac cactcgaagt ggggtgtgat cggcctggcc tccttcgggc gtctcgagct
     3421 cgtcgagcgc ctcgtcaccg actcgggcct gccgggaccg gcccgggagg cgatcgaggc
     3481 gctggggtgc cgggtcgacc tggtcgacct cctggaggac cccgcccggc ttgacggcgc
     3541 cggctcgcgc tcgcgaccct cacaccatgg ctgagaccga cagcgccgac agcgccgaca
     3601 gcgccg
//
LOCUS       DATAIX010000339         2419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_127082, whole genome shotgun sequence.
ACCESSION   DATAIX010000339 DATAIX010000000
VERSION     DATAIX010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127082"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..886
                     /locus_tag="VHM65_05040"
     CDS             293..886
                     /locus_tag="VHM65_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705104.1"
                     /translation="MKRTTLDKLLSSLGLAMTALLIAAGALMTWASSFVAGQVHDQLA
                     GQKFTMPVGAAIEDPLSKPYLQEFAGQEMTTGSQAKAYADHYILVHMNKAGAGRTYEE
                     VSGEFIAMSSDPAADKDKLAELGRLRQTLFMGNTLRGLLLNAYAFGTMATILGIGAIV
                     SFVGAAVMPLLSLLGLRHARTASTPAAPVGTREPVTS"
     gene            complement(951..1769)
                     /locus_tag="VHM65_05045"
     CDS             complement(951..1769)
                     /locus_tag="VHM65_05045"
                     /inference="COORDINATES: protein motif:HMM:NF039405.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6308 family protein"
                     /protein_id="HEX2705105.1"
                     /translation="MNQHESRSGLLPADWWVVEDAVVARASRRTHEALADVRIPARLA
                     EYYRRGSSYAGATFLDLEPHAPYAITPGDVLALNTLNVPVGAGAIRRLLAPGGQSAAI
                     SQLLASDALRVGADLRTADRGVFEAMEQLYVAVKSAISGEAVAHPNAWVTASTLCARK
                     RPELFPVRDYVVCRHLGLLDGGGRYTQDWLVFRALMSDAGVRAGVESLIARTEGPGIE
                     VGDRHALLRHLDVVLWMHAREVRPAGTGAARAERRSAARGDGLDIPGQGQWLLT"
     gene            complement(1804..>2419)
                     /locus_tag="VHM65_05050"
     CDS             complement(1804..>2419)
                     /locus_tag="VHM65_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HEX2705106.1"
                     /translation="VFLDRTWRMHPAITGYVSQMFYDDRLESAPGLDRQRVQAPGRLS
                     GAGLRWVPVEHDGCVADSTAEARVVLELVTDLLTGSWTDAQGSRAALTEQDVLVVAPY
                     NAAVAMLRAYLPPGVRVGTVDKFQGQQAPVVVYSMTSSNAAVAPRGVDFLYDLHRFNV
                     AISRAKALTVVVGSPALLDAAVHSPEQLRAVNALCRYVDLASGE"
BASE COUNT          404 a          875 c          803 g          337 t
ORIGIN      
        1 aacgaggaca ttggagggac gtactacgaa tgggagggca ccaaccgccg tgagcacgtc
       61 tctgttcgac gcagccttgt cggcgaggac ggcgagtgga tcgacccgga cccccacaca
      121 tcctgacgct ggtcctcacc ggaggcctca cggacgagca gcccggcgac cctcgtgcgg
      181 caacgtgaca aggcactcac ctgggcgggg acgtttgtcc cgagccccga gcactagttg
      241 agtgttcaat gaaagtccga cccacacccg tcacctcgac tcaaggaacg caatgaagcg
      301 gacgaccctc gacaagctgc tctcctctct cggcctggcc atgaccgcgc tgctcatcgc
      361 ggctggggcc ctgatgacct gggcctcctc cttcgttgct ggtcaggtcc acgaccagct
      421 cgccggacag aagttcacga tgcccgtggg agcggcgatc gaggaccccc tcagcaagcc
      481 ctacctgcag gagttcgcgg gacaggagat gacgacgggc agccaggcca aggcgtacgc
      541 cgaccactac atcctcgtcc acatgaacaa agccggcgcg ggcaggacct atgaggaggt
      601 cagcggcgag ttcatcgcca tgtccagtga cccggcagcc gacaaggaca aactcgccga
      661 gctcgggcgg ctgcgccaga ccctcttcat gggcaacacg ctgcggggcc tgctgctcaa
      721 cgcgtacgcg ttcggcacga tggccacgat cctcggcatc ggcgccatcg tctccttcgt
      781 cggagcggcc gtgatgcccc tcctcagcct gctgggcctg cgccacgcgc gcacagccag
      841 cacccccgcc gctccggtcg gcacgcggga gcccgtcacg agctgagcca tcccacacca
      901 tgcgcaggcc cggctgaggc cattcctcgc cgggcttggg ccggggtggc tcaggtcaac
      961 aaccactgcc cctgacccgg tatgtcgagt ccgtcacctc gcgctgccga ccgcctctcg
     1021 gcccgggccg caccggtgcc cgccggcctc acctcacggg catgcatcca cagcacgaca
     1081 tcgaggtggc gcaggagggc gtgccggtct ccgacctcga tgcccggccc ctcggtgcgg
     1141 gcgatgagac tctcgacccc ggcccggaca ccggcgtccg acatgagggc ccggaagacc
     1201 agccagtcct gggtgtagcg cccgcccccg tcgagcagcc cgagatggcg gcagacgacg
     1261 tagtcacgca ccgggaacag ctcggggcgc ttgcgagcgc acagtgtgga ggcggtcacc
     1321 cacgcattgg gatgagcgac ggcctcaccc gagatcgcgg acttgaccgc gacatagagc
     1381 tgctccatcg cctcgaagac gccccggtcg gccgtcctca ggtcggctcc tacccgcagc
     1441 gcgtcgctgg cgagcagctg gctgatcgcg gcgctctggc cccccggcgc gagcagtcgc
     1501 cggatcgccc ccgctccgac ggggacgttg agggtgttga gggcgaggac atccccggga
     1561 gtgatcgcgt acggcgcgtg cggctccaga tcgaggaagg tcgccccggc gtaggacgag
     1621 ccacggcggt agtactcggc caggcgcgcc ggaatgcgca cgtcggcgag cgcctcgtgc
     1681 gtgcgacgcg aggcccgagc cacgacggcg tcctcgacca cccaccagtc ggccggcaac
     1741 agcccgctgc ggctctcgtg ctggttcatc ggcgctcctg gagtcggcct gttgaccccc
     1801 tttctactcc cccgaagcca ggtcgacata ccggcacagg gcattgacgg cgcgcagctg
     1861 ctccggtgag tgcacggcgg catcgagcag ggcggggctc ccgacgacga ccgtgagggc
     1921 cttggcccgg gagatcgcga cgttgaaccg atgcaggtcg tagaggaagt cgaccccgcg
     1981 cggagccacg gcggcgttcg agctcgtcat cgagtagacg acgaccgggg cctgctgccc
     2041 ctggaacttg tcgaccgtcc cgacccgaac ccccgggggc aggtaggcgc gcagcatcgc
     2101 caccgcagcg ttgtagggcg cgacgacgag gacgtcctgc tcggtcagtg ccgccctgct
     2161 cccctgcgcg tcggtccatg acccggtgag caggtcggtg accagctcga gcaccacgcg
     2221 ggcttcagcg gtgctgtcgg caacgcagcc gtcgtgctcg acggggaccc agcgcagtcc
     2281 cgcacccgac agcctccccg gtgcctgaac ccgctgacgg tccagccccg gagccgactc
     2341 gagccgatcg tcgtagaaca tctgcgagac atagccggtg atggccgggt gcatccgcca
     2401 cgtccggtcg aggaacacg
//
LOCUS       DATAIX010000340         4041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_167697, whole genome shotgun sequence.
ACCESSION   DATAIX010000340 DATAIX010000000
VERSION     DATAIX010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167697"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..367
                     /locus_tag="VHM65_05055"
     CDS             62..367
                     /locus_tag="VHM65_05055"
                     /inference="COORDINATES: protein motif:HMM:NF014022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEX2705107.1"
                     /translation="MLVAFSVAPAVAEDESGSVSHAVAEAIRVVRGSGLPNHTDSMFT
                     TIEGEWDACMAVVRAACDAVAAVSPRVSLVLKADIRPGFAGQLASKVDRVERILGSS"
     gene            393..917
                     /locus_tag="VHM65_05060"
     CDS             393..917
                     /locus_tag="VHM65_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2705108.1"
                     /translation="MPAPRRLTFDPIRRAAELWRARWGDDAQDVPMATATSIMRVQQL
                     LLTDFDALVGRHGLTFARYEALVLLAFSSAGRLPMSVVGQRLMVHPTSATNIVQRLVA
                     QGFVERVANPADGRGALAVVTDAGRQVMEEATTDLVEAGFGLRMLTAAEHTDLFRILR
                     KIRLAAGDFAEGHL"
     gene            955..2682
                     /locus_tag="VHM65_05065"
     CDS             955..2682
                     /locus_tag="VHM65_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX2705109.1"
                     /translation="MTDAESTGRERWQARYDAAQSRDQVRDSDFTTLSGMPVDAVYGP
                     PGGRDALEADPRIGWPGEFPFTRGLYPTGYRGRTWTIRQFAGFGNAEQTNERYRMILA
                     RGGGGLSVAFDMPTLMGRDSDDPLGLGEVGHCGVAIDSAADMEVLFRDIPLADITTSM
                     TISGPAVPVFCMYLVAAERQGVDPGVLNGTLQTDIFKEYIAQKEWLFAPEPHLRLIGD
                     LMEYCAEHIPAYKPLSVSGYHIREAGSTAAQELAFTLADGFGYVELGLSRGLDIEVFA
                     PGLSFFFDAHIDFFEEIAKFRAARRIWARWMRDVYGARTDKAQWLRFHTQTAGVSLTA
                     QQPYNNVVRTAVEALSAVLGGTNSLHTNALDETLALPTEQSAEIALRTQQVIMEETGV
                     VNVADPLGGSWYVEALTDRMEAEAEAIFDTIRRMGEIGRVPGADGQIAHEIGPMTSGI
                     LRGIEEGWFMGEIADAAFQHQLALEKGDKRIVGVNCYPESVSHDIEILRVSHTVEQVQ
                     VRALADRRRDRDAAAASAALAEMVQAARSGANMIPAMLDAARAEATLGEICNALRDEW
                     GIYREPARF"
     gene            2782..3447
                     /locus_tag="VHM65_05070"
     CDS             2782..3447
                     /locus_tag="VHM65_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705110.1"
                     /translation="MTVLDVRPLAVRRFDVRRFFVALVALLGLVLAGVGVWLAALVGP
                     SGTIVFRGTSGEPLLLTPDILNRVDVPVTVQASSASGGPVWLGLSTPQDVAAAVGAGR
                     HDRVVVAAFPSRRLATTTEGDVALADPRPLQVWARTASGSASATIELDQAAAPQSVLV
                     VPGQPGPVEVTLTWVKKRWFVEAAMVVVAGLGIAGFGGGWLYQQLRRDPVPRPAEDEQ
                     EGP"
     gene            3444..>4041
                     /locus_tag="VHM65_05075"
     CDS             3444..>4041
                     /locus_tag="VHM65_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705111.1"
                     /translation="MSAAGPTMLRRALAVCGAALLVLALSACGPLNAMVGIKDAPAGT
                     TGGALTSTRATEISTELLARASTAATTPGDAGVGQRAAAYAGAALAAANAAAKLAPTQ
                     TAAERSAAEMSPDAPVVLAVSRGPGYPRSMVVQSTLAQSKLPVLHLLVTADVRTPYKI
                     AASATMLSGAQVRAFDPLAAGSPAVGDGAGLLAVPSDLA"
BASE COUNT          545 a         1425 c         1460 g          611 t
ORIGIN      
        1 gaatggagcc tgtccgaggg gggcgacgcg cggagccggg cactcggcat accgtggggt
       61 catgcttgtt gccttctccg tagcccctgc cgtcgccgag gacgagagcg gatcggtgag
      121 ccacgcggtc gccgaggcca tccgggtcgt gcgcgggtcc ggcctgccga accacaccga
      181 ctcgatgttc acgacgatcg agggggagtg ggatgcctgt atggcggtcg tcagggccgc
      241 gtgcgacgcg gttgccgcgg tgagcccccg ggtcagcctg gtgctcaagg ccgacatccg
      301 gcccgggttc gccggccagc tcgcaagcaa ggtcgaccgg gtcgagcgaa tcctcggctc
      361 gtcctgagcg ccggtcggac gacggtatcg ccgtgcctgc cccgcgccga ctgaccttcg
      421 acccgatccg tcgggcggcc gagctctggc gcgcccggtg gggcgacgac gcgcaggacg
      481 tgccgatggc gacggcgacc tcgatcatgc gggtgcagca gctcctgctc accgacttcg
      541 acgcgctggt ggggcgccat ggcctgacct tcgcgcgcta cgaggcgctc gtgctgctcg
      601 ccttctcctc cgctgggcgg ctgccgatgt cggtggtcgg gcagcggctc atggtccacc
      661 cgaccagcgc gacgaatatc gtccagcgcc tcgtggcgca gggattcgtc gagcgcgtgg
      721 ccaacccggc cgacgggcgc ggggcgcttg ccgtggtcac cgacgccggc cgccaggtca
      781 tggaggaggc gacgaccgac ctcgtcgagg ccgggttcgg gctgaggatg ctcaccgctg
      841 ccgagcacac ggacctgttc cggatcctgc gcaagatccg cctggccgcc ggtgacttcg
      901 ccgagggcca tctttagtag gacgtcctag tagttcgggc gtatcctccc tggtatgacg
      961 gatgccgaat cgacgggtcg cgaacgctgg caggcccggt atgacgctgc gcagtcgcgc
     1021 gaccaggtgc gggactcgga cttcacgacg ctgtcgggga tgccggtgga cgcggtctac
     1081 gggccgcccg gtgggcgcga cgccctggag gccgacccgc ggatcggctg gccgggggag
     1141 ttcccgttca cccgggggct ctaccccacg ggatatcgag ggcggacgtg gacgatccgc
     1201 cagttcgccg gcttcggcaa cgccgagcag accaacgagc gctatcggat gatcctggcc
     1261 cgcggcggcg ggggtctgtc ggtggcgttc gacatgccga cgctcatggg ccgcgactcc
     1321 gatgacccgc tcggcctcgg tgaggtcggg cactgtggcg tcgcgatcga ctccgcggcc
     1381 gacatggagg tgctgttccg ggacatcccc ctcgctgaca tcacgacgtc gatgacgatc
     1441 tcgggaccgg ccgtcccggt gttctgcatg tacctcgtcg ccgccgagcg ccagggcgtc
     1501 gatccgggcg tgctcaacgg gacgctgcag accgacatct tcaaggagta catcgcccag
     1561 aaggagtggc tcttcgcgcc cgagccccac ctgcggctca tcggcgatct catggagtac
     1621 tgcgccgagc acatcccggc atacaaaccc ctgtccgtct ccggctacca catccgcgag
     1681 gcaggctcga cggcggcgca ggagctggcc ttcaccctcg ccgacgggtt cggctacgtc
     1741 gagctggggc tctcgcgcgg tctcgacatc gaggtcttcg cgccgggcct gtcgttcttc
     1801 ttcgacgcgc acatcgactt cttcgaggag atcgcgaagt tccgcgcggc ccggcgcatc
     1861 tgggctcgct ggatgcgcga cgtgtatggc gcacgcaccg acaaggcgca gtggctgcgg
     1921 ttccacacgc agaccgcagg tgtctcgctg acggcccagc agccctacaa caacgtcgtc
     1981 cgaacggcgg tcgaagcgct ctccgcggtc ctcggcggga ccaactcgct gcacacgaac
     2041 gccctcgacg agacgctggc cctgccgacc gagcagtcgg ccgagatcgc gctgcggacc
     2101 cagcaggtca tcatggagga gaccggcgtc gtcaacgtcg ccgacccgct cggcggttcc
     2161 tggtatgtcg aggccctcac cgaccgcatg gaggccgagg cggaggcgat cttcgacacg
     2221 atccgcagga tgggtgagat cgggcgcgtc cccggcgctg acggtcagat cgcgcacgag
     2281 atcggaccga tgacctcggg gatcctgcgc ggcatcgagg agggctggtt catgggcgag
     2341 atcgccgacg cggccttcca gcaccagctg gccctcgaga agggcgacaa gcggatcgtc
     2401 ggcgtcaact gctaccccga gtcggtgagc cacgacatcg agatcctgcg agtcagccac
     2461 acggtcgagc aggtgcaggt gagggcgctg gccgaccgtc ggcgtgaccg tgacgcggct
     2521 gccgcgtcag cagcgcttgc cgagatggtg caggccgcgc gcagcggggc caacatgatc
     2581 cccgcgatgc tcgacgcggc gcgggctgag gccacgctgg gtgagatctg caacgccctg
     2641 cgggacgagt ggggcatcta ccgggaaccg gcacgcttct aggcgacggc tggccggccc
     2701 ctcgcgcggc agggcgcgat tggcttgcgc cctgcgcccc ggcctatggt ggatggtccg
     2761 ccagcccagt ccgaagaggt catgaccgtg ctcgatgtcc gcccgctcgc cgtgcgccgc
     2821 tttgacgtgc ggcgtttctt cgtggccctg gtggcccttc tcgggctcgt cctcgcgggg
     2881 gtcggagtct ggctggcggc cctggtcggg ccctccggga ccatcgtctt tcgggggacg
     2941 tccggagagc cgttgctgct gacacccgac atcctcaacc gggtcgacgt cccggtgacg
     3001 gtgcaggcgt cgtcggcctc cggcggcccc gtctggctcg gcctgagcac gccccaggac
     3061 gtcgccgccg ccgtcggcgc gggtcgtcac gaccgcgtgg tcgtcgccgc tttccccagc
     3121 cgtcggctcg ccacgacgac cgaaggcgat gtggcgctgg ccgacccgag gccgctgcag
     3181 gtctgggcgc ggaccgcatc cggaagcgca tcggccacca tcgagctcga ccaggcggcg
     3241 gctccccagt cggtgctcgt cgtgcccggt cagcccggcc cggtcgaggt caccctgacc
     3301 tgggtcaaga agcgatggtt cgtcgaggcg gccatggtcg tcgtggccgg cctgggcatc
     3361 gcgggtttcg ggggtggctg gctctaccag cagctgcgcc gtgacccggt gccgcggccc
     3421 gccgaggacg agcaggaggg cccgtgagcg ccgccgggcc gacaatgctg cgccgggccc
     3481 tcgcggtgtg cggcgccgcg ctgctggtcc tcgcactgtc ggcgtgtggt ccgctcaacg
     3541 ccatggtcgg catcaaggac gcaccggccg gcacgaccgg gggagccctg acctcgaccc
     3601 gggccacgga gatctcgacc gagctgctgg cccgggcatc cacggccgcc acgacccccg
     3661 gcgacgcggg cgttgggcaa cgggccgcgg cgtatgccgg tgcagcgctg gcggcggcca
     3721 acgccgccgc caagctggcg ccgacccaga ccgcggccga gcggtccgcg gccgagatga
     3781 gtccggacgc tccggtcgtc ctcgcggtat cccgcggccc cggctacccg cgctcgatgg
     3841 tcgtgcagtc gaccctggcc cagtccaagc tgcccgtcct gcacctgctc gtcacggcgg
     3901 acgtgcgcac gccatacaag atcgcggcca gcgccaccat gctctctggg gcacaggtgc
     3961 gggcctttga cccgctcgcg gccgggtcgc cggcggtcgg cgacggcgcc gggctcctcg
     4021 ccgtgccgag cgacctcgcg g
//
LOCUS       DATAIX010000341         3069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_48376, whole genome shotgun sequence.
ACCESSION   DATAIX010000341 DATAIX010000000
VERSION     DATAIX010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48376"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..179
                     /gene="rpmG"
                     /locus_tag="VHM65_05080"
     CDS             12..179
                     /gene="rpmG"
                     /locus_tag="VHM65_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003829846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX2705112.1"
                     /translation="MAKSTDVRPKITMACVDCKHRNYITKKNRRNNPDRLELAKFCPT
                     CGKHTAHRETR"
     gene            220..663
                     /locus_tag="VHM65_05085"
     CDS             220..663
                     /locus_tag="VHM65_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2705113.1"
                     /translation="MPVNAELAGRAYPPTSPYGVSAAKIREFAEAVGSPDLAHVDAGV
                     ARARGYRDVIAPPTFAVLIAQQCDAQLIRDPEAGIDFSRVVHGEQAFVHHRPLMAGDD
                     IVGVLHVDSVKAAGGHTMVTTRSELTTTSGEAVCTATSTIVVRGE"
     gene            671..1117
                     /locus_tag="VHM65_05090"
     CDS             671..1117
                     /locus_tag="VHM65_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX2705114.1"
                     /translation="MSQDTTPAGIRLAGDVEVGQTLPETTVHIDRATLVQYAGASLDR
                     NRIHWDERFAVSVGLPDVIAHGMFTMGAAIRIVTAWAGDAGRIVQYGTRFTKPVVVPY
                     VEGADLVVTGVVKALDPETRRATVDLTATCRDERVLGRCSAVVQLD"
     gene            1157..2227
                     /locus_tag="VHM65_05095"
     CDS             1157..2227
                     /locus_tag="VHM65_05095"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX2705115.1"
                     /translation="MTEDFDAPLAPLTTLRVGGPARRLVTVTSTDELVDAVREVDDAG
                     EPLLVLGGGSNLLICDDGFAGTVVRVATRGVAVESGDWCGGANVRVAAGEPWDDVVVR
                     AVDEGWAGVEALSGIPGSTGATPVQNVGAYGQEVAQTIAQVRVWDRGSEAVRTMAGGD
                     CGFTYRGSLFKSTSRYVVLDVLFQLRVADRSDPIRYADLAAGLGVEAGARVRLAEARA
                     AVLEQRRRRGMVLDPGDHDTWSCGSFFTNPLLAPDAFTELGRRVRERLGPGATPPQYP
                     ASAGAVKTSAAWLIERAGFGKGYGLPGPAAVSGKHALALTNRGGASAADLVALARQVR
                     NGVRDAFGVELVPEPVLVGVTL"
     gene            complement(2259..2690)
                     /locus_tag="VHM65_05100"
     CDS             complement(2259..2690)
                     /locus_tag="VHM65_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705116.1"
                     /translation="MRNKTISVAEFGHTITVLAIQRNWPWPAGYEASSAALELVAVQM
                     TWVAGTKYTAAISPDQFVLVTGATLSRPDTTINALLKARKYALLPAKVLTGKKATGWL
                     VFKVDPKGAKALVLRYDRPKALVTSTNQTIPAKSFSVKLVG"
     gene            2680..>3069
                     /locus_tag="VHM65_05105"
     CDS             2680..>3069
                     /locus_tag="VHM65_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705117.1"
                     /translation="MFRNLTAAAGDGLVGDDVADDVTKVDVDADAVGLDDPVELWAAA
                     VVVLEDAPEPAEQPARATAPDRATAPARATAPGRARPTDGFIESSSRRGQVAAMGCRL
                     RRPWSRAVSVQHARWFRAAPNRQPGRQA"
BASE COUNT          430 a         1094 c         1106 g          439 t
ORIGIN      
        1 aggttttctc cgtggccaag agcaccgacg tccgcccgaa gatcacgatg gcgtgcgtgg
       61 actgcaagca ccgcaactac atcacgaaga agaaccgccg gaacaacccc gaccggctcg
      121 agctcgcgaa gttctgcccc acctgcggca agcacaccgc gcaccgcgag acccgctgac
      181 gggcccgtct cgcgcgccca ttcccattaa ggttggcgca tgcctgtcaa tgctgagctc
      241 gccgggcgcg cctacccgcc cacctccccg tatggcgtca gcgcggcgaa gatccgcgag
      301 ttcgccgaag ccgtgggatc acccgacctg gcccacgtcg acgccggggt ggcccgggcc
      361 cgcggctacc gcgatgtcat cgccccgccg acgttcgcag tcctcatcgc ccagcagtgc
      421 gacgcccagc tgatccgcga ccccgaggcc gggatcgact tcagccgggt cgtccacggc
      481 gagcaggcct tcgtccacca ccgcccgctc atggccggtg acgacattgt cggggtcctg
      541 cacgtcgact cggtcaaggc ggccgggggc cacacgatgg tgacgacccg cagcgagctg
      601 acgacgacga gcggcgaggc cgtctgcacc gcgacctcga cgattgtggt ccggggagag
      661 tgagcacgcg atgagccagg acaccacgcc cgccgggatc cgcctcgccg gcgatgtcga
      721 ggtcggccag accctgcccg agacgaccgt ccacatcgac cgcgcgacgc tcgtgcagta
      781 cgccggcgcc tccctcgacc gcaaccgcat ccactgggac gagcggttcg cggtcagtgt
      841 gggcctgccg gacgtcattg cccacggcat gttcaccatg ggcgccgcca tccgcatcgt
      901 cacggcgtgg gccggcgacg ccgggcgcat cgtgcagtac ggcacgcgct tcaccaagcc
      961 ggtcgtcgtc ccgtatgtcg agggcgcgga tctcgtcgtg accggcgtcg tcaaggctct
     1021 cgatcccgag acgcgcaggg ccaccgtcga cctcaccgcg acgtgccggg acgagcgggt
     1081 cctcgggcgc tgctcggccg tcgtgcagct cgactgaccc ggtcccgcca tacccgactg
     1141 aaccggtgac ggctcgatga ccgaggactt cgacgcaccc ctggccccgc tcacgaccct
     1201 gcgcgtcggc ggacccgcgc gcaggctcgt cacggtcacg tcgaccgacg agctcgtcga
     1261 cgcggtgcgc gaggtcgacg acgcggggga gccgcttctc gtcctcggtg gtgggtcgaa
     1321 cctgctgatc tgcgacgacg ggttcgccgg cacggtggtc cgcgtggcta cgcgcggcgt
     1381 cgcggtcgag tccggcgact ggtgcggtgg cgcgaatgtc cgcgtggccg cgggtgagcc
     1441 ctgggacgac gtcgtggtcc gggcggtcga cgaaggatgg gccggtgtcg aggccctgtc
     1501 gggcatcccc ggcagcacgg gcgccacgcc cgtgcagaat gtcggggcct acggccagga
     1561 ggtcgcccag acgatcgccc aggtccgggt gtgggaccgc gggagcgagg cggtccgcac
     1621 catggccggc ggcgactgcg gcttcaccta ccgcggctcg ctgttcaaga gcacgagccg
     1681 gtatgtcgtc ctcgacgtgc tcttccagct gcgggtcgcc gaccgctccg acccgatccg
     1741 gtatgccgac ctcgcggcgg gactcggcgt cgaggcaggc gcccgcgtgc ggctcgccga
     1801 ggcccgcgcg gccgttctcg agcagcgccg gcgccggggc atggtgctcg acccgggtga
     1861 tcacgacacg tggagctgcg ggtcgttctt caccaacccg ctgctggcac cggatgcctt
     1921 caccgagctc gggcggcggg tccgcgagcg gctgggtcca ggcgcgacgc ctccgcagta
     1981 ccccgcctcg gcaggggcgg tcaagacgag tgcggcctgg ctcatcgaac gggctggttt
     2041 cggcaagggc tacgggctgc ccggcccggc ggccgtctcg ggcaagcacg cgctggccct
     2101 gacgaaccgt ggcggggcga gcgcagccga cctcgtggcg ctggcccgcc aggtccgtaa
     2161 cggggtgcgg gacgccttcg gcgtcgagct ggtccccgag ccggtcctcg tcggcgtgac
     2221 cctgtgaccg gtcgcccgac gtgcatggcc gcgcacgctc agcccacgag cttgaccgag
     2281 aacgacttcg ccgggatggt ctggttcgtc gacgtcacga gcgccttggg ccggtcgtag
     2341 cgcaggacca gggccttggc gcccttgggg tcgaccttga agacgagcca gccggtcgcc
     2401 ttcttgccgg tgaggacctt ggcggggagc agcgcgtact tgcgggcctt gaggagggcg
     2461 ttgatcgtcg tgtcgggccg gctgagcgtg gcgcccgtca cgaggacgaa ctggtcgggc
     2521 gagatggccg cggtgtactt cgtgcccgcg acccacgtca tctggacggc gacgagctca
     2581 agggcggccg agctggcctc atagcccgcg ggccagggcc agttgcgctg gatcgccagg
     2641 accgtgatcg tgtgcccgaa ctcggcgacc gagatcgtct tgttccgcaa cctgaccgcg
     2701 gcggccggtg acgggcttgt cggtgacgac gtcgccgacg acgtcaccaa ggtcgacgtc
     2761 gatgcggacg ccgtcgggct cgatgaccct gtcgagctct gggcggcggc cgtcgtcgtg
     2821 ctggaggacg ctcccgaacc ggccgagcag ccggccagag ccactgcgcc ggacagggcc
     2881 actgcgccgg ccagagccac tgcgccgggc cgcgcgcgac ccacggatgg cttcatcgaa
     2941 tcgtcctcac gtcgggggca ggtagcggcg atggggtgcc ggctgaggcg tccctggagc
     3001 cgcgccgtct cggtccagca tgcgcgatgg ttccgagcgg cgccaaatcg gcagccgggt
     3061 cgccaagcc
//
LOCUS       DATAIX010000342         1771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_167773, whole genome shotgun sequence.
ACCESSION   DATAIX010000342 DATAIX010000000
VERSION     DATAIX010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167773"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1417
                     /gene="hutI"
                     /locus_tag="VHM65_05110"
     CDS             <1..1417
                     /gene="hutI"
                     /locus_tag="VHM65_05110"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX2705118.1"
                     /translation="CEGALADFVAVGLTSPRTAGSEPGQVLLSATSADVTDVVVGGRR
                     VVADRQHRLGDVGQLLADAIHPLGGLMSSLAVTGIGELVTNDSSLDATRDGDHLGLLH
                     DVTLVVDNGRVAWIGPSGADPPADRAVDLGGRAVVPGFVDSHTHLVFAGERSAEFAAR
                     MSGIPYDGGGIAATVAATRAASDDELRALLAGRVAALRALGTTTFEVKSGYGLAVEDE
                     ARLLRLAREVTAETTFLGAHVVPPEYRGDRDGYVGLVTGPMLAACAPYARWVDVFCEP
                     HSPHAFTGDEARAVLIAGRLAGLEGRVHGNQLGPGPGVQLAVELGAASVDHCTYLADR
                     DVDALVSARDATVATLLPGVEFSTRSPYPDARRLIDAGASVALASDCNPGTHYSGSIP
                     FVLALAVREMHLTPAEALRAATVGGAAALRRPELGRLTIGSPAHLTVVDAPTYVHLVY
                     RPGMPLSRAFGDAVATSTADA"
BASE COUNT          206 a          653 c          665 g          247 t
ORIGIN      
        1 gtgtgagggt gcgctcgccg acttcgtcgc cgtcgggctg accagcccgc ggacggccgg
       61 gagcgagccc gggcaggtcc tgctcagcgc gacctccgcc gacgtcaccg acgtcgtcgt
      121 cggcggccgg cgcgtcgtcg ccgaccggca gcaccgcctc ggtgacgtcg ggcagctgct
      181 cgcggacgcc atacaccctc tgggaggact catgagcagc cttgcagtga ccggtatcgg
      241 ggagctcgtc accaacgact cgagcctgga cgccacccgg gacggggacc acctcggcct
      301 cctgcacgac gtcaccctcg tcgtcgacaa cggccgggtc gcctggatcg gccccagcgg
      361 agccgatccg cccgccgacc gcgcggtcga cctcggcggc cgggcggtcg tgccggggtt
      421 cgtcgacagc cacacccacc tcgtcttcgc cggggagcgc tcggccgagt tcgccgcccg
      481 gatgagcggg atcccgtatg acggcggcgg tatcgcggcg accgtcgccg cgacccgggc
      541 ggcgagcgac gacgagctgc gggcgctgct ggccgggcgg gtcgccgcgc tgagggcact
      601 cgggaccacg accttcgagg tcaagagcgg ctacggcctt gccgtcgagg acgaggcgcg
      661 cctgctgcgc ctcgcccgcg aggtgaccgc ggagacgacc ttcctcggcg cccatgtcgt
      721 cccgccggag taccgaggcg accgggacgg gtatgtcggt ctcgtcaccg gccccatgct
      781 ggcggcgtgc gcgccatacg cgaggtgggt cgacgtcttc tgcgagcccc acagcccgca
      841 cgcctttacc ggcgacgagg cacgggcggt gctcatcgcc ggccggctgg ccgggctcga
      901 ggggcgcgtc cacggcaacc agctcggccc ggggcccggc gtccagcttg ccgtcgagct
      961 cggggcggcg agcgtggacc actgcaccta cctcgccgac cgcgatgtcg acgcgctcgt
     1021 gtccgcgcgg gacgcgacgg tcgcgacgct gctgcccggg gtggagttct cgacccgctc
     1081 gccctacccc gatgcccgcc ggctcatcga cgcgggggcg tcggtggccc tggccagcga
     1141 ctgcaacccg ggcacgcact acagcgggtc gatcccgttc gtcctggcac tcgcggtccg
     1201 ggagatgcat ctcaccccgg ccgaggcgct gcgcgccgcg acggtcgggg gtgccgcggc
     1261 gctgcgccgc cccgagctcg ggcgtctcac catcgggagc ccggcccacc tcaccgtcgt
     1321 ggacgccccg acgtacgtgc acctcgtcta ccgtccgggg atgcctctct cgcgggcctt
     1381 cggggacgct gtcgccacct cgactgccga tgcttgagtc ctgcaaggat cgcccgagct
     1441 gaggagcgga cctggccggt cggcgccggc cggttcggcc tcggtgcccg ggctgcagcg
     1501 attgaggtgg atctggccgg tgggggtcgg ccggttcggc ctcggtgtcc gggctgcagc
     1561 gatcgaggtg gatctggccg gtgtgggccg gccgggtggg tgatgatggc acggtgagct
     1621 cccctgaagg cgagcacgtt cggcggcatg ctggcgatcg cgctgaacac cgccggcccg
     1681 ccggctgagt gcccctgccc ccagcggagc cggccagcgc cgggagcgag ggggtcagtc
     1741 ttggtcggtc agcgccttgg gcaggtgccc c
//
LOCUS       DATAIX010000343         1777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_18260, whole genome shotgun sequence.
ACCESSION   DATAIX010000343 DATAIX010000000
VERSION     DATAIX010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18260"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..639
                     /locus_tag="VHM65_05115"
     CDS             61..639
                     /locus_tag="VHM65_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2705119.1"
                     /translation="MQDVEVIESAEAATAALDPVRARLLGELAVPASAAGLAARVGIA
                     RQKVNYHLKALEAHGLVELSEERRHGGITERVLQASAASYVVSPAAVSASAADPDANA
                     DHLSAGYLVALAGRLVREVGALTRRAGASGKRLPTLTIDTQIGFRSAADRAAFADDLT
                     AAVLDLAARYHHDDGRPHRLVVAAHPLPEERA"
     gene            636..1427
                     /locus_tag="VHM65_05120"
     CDS             636..1427
                     /locus_tag="VHM65_05120"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX2705120.1"
                     /translation="MSTTPNVPYRLEFSVEVPGTPEQVWQAIATAKGMSAWFLPTEME
                     EREGGSLHFTMGPEMGSDGQVTGWDPPRRLVYEEDWAALMGKDPDALSPLTSEFLVEA
                     QSGGTCVVRVTSSGFGTGAAWESEFWDDMGPNWMPFFDNLRLYLSHFPGQEATQLEVT
                     ASHPGDAVALWSTLHDALGLGDEATTVEVRGVTGTIERVGERQTLVRLTAPVPGMLSV
                     FAYGEGDGKATAGVRAYLFSADAADYVRREEPAWQAWLQGLSVPC"
BASE COUNT          244 a          647 c          645 g          241 t
ORIGIN      
        1 gaacacgacg tctgactgcg cgcccttgac aagacttctt gtcaagggca cggtggcgtc
       61 atgcaggacg tcgaggtcat cgagagcgcg gaggcagcca ccgccgcact ggatccggtc
      121 cgggcccggc tgctcggcga gctggccgtc ccggcctccg ccgccgggct cgccgcccgg
      181 gtcggcatcg cacgccagaa ggtcaactac cacctcaagg cgctcgaggc gcacggcctg
      241 gtggagctgt ccgaggagcg ccggcacggg ggcatcaccg agcgggtgct gcaggcgtcg
      301 gcggcgtcgt acgtcgtgtc gccggcggcc gtcagcgcgt ccgcggccga cccggacgcc
      361 aacgccgacc acctgtcggc gggctacctc gtcgcgctgg ccggccggct ggtgcgcgag
      421 gtcggcgccc tgacgcgccg ggccggcgcg tccggcaagc gcctgccgac gctgaccatc
      481 gacacgcaga tcggcttccg gtcggccgcc gaccgggctg ccttcgcgga cgacctgacc
      541 gccgcggtgc tcgacctggc cgctcgctac caccacgacg acgggcgccc gcaccggctc
      601 gtcgtcgccg cacaccccct tcctgaggag cgcgcatgag cactaccccg aacgtcccgt
      661 accgcctgga gttcagcgtc gaggtcccgg gcaccccgga gcaggtctgg caagccatcg
      721 ccaccgccaa gggcatgagc gcctggttcc tgccgaccga gatggaggag cgcgagggcg
      781 gctccctgca cttcaccatg ggccccgaga tgggctcgga cggccaggtc accggctggg
      841 acccgccgcg ccgcctcgtc tacgaggagg actgggccgc cctcatgggc aaggacccgg
      901 atgcgctcag cccgctgacg tcggagttcc tcgtcgaggc gcagtccggc ggcacctgcg
      961 tcgtgcgcgt caccagcagc ggcttcggga ccggcgccgc ctgggagtcg gagttctggg
     1021 acgacatggg ccccaactgg atgccgttct tcgacaacct gcgcctctac ctgtcgcact
     1081 tccccggcca ggaggccacg caactggagg tcaccgcctc ccaccccggc gacgcggtgg
     1141 cgctctggtc gaccctgcac gacgcgctgg ggctgggcga cgaggccacg acggtcgagg
     1201 tgcgcggcgt caccggcacg atcgagcgtg tcggcgagcg gcagacgctg gtccggctca
     1261 ccgcgccggt gcccgggatg ctcagcgtct tcgcgtacgg cgagggcgat ggcaaggcga
     1321 cggcgggtgt gcgggcgtac ctgttctccg ccgatgcggc ggactacgtg cggcgcgagg
     1381 agccggcctg gcaggcctgg ctgcaggggc tctccgtccc atgctgaatc aggtggccga
     1441 gggcgtctgg gttcggcaga gcgcgtgggt ctgaagctga tcggactcct caccccgcgg
     1501 aaggcggagc catgcccggg cgagatcgtc gagcatcagg cgcacgccat cggccacgcc
     1561 ggggtcccgc tcgccgaccg tggcgtcatg ctcgccggcg acatgctctc cgacgtcctg
     1621 atcccgctcc tggacccctc gccgcgccga tcagggttga cccggcgtca aggtcaagcc
     1681 aggtccaacg cgatggtagg gccggcggcg gtctcggcgg agaggccggg aagttggccg
     1741 atctcggtcc aagtgaggcc gggccacccg atccgag
//
LOCUS       DATAIX010000344         1794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_48484, whole genome shotgun sequence.
ACCESSION   DATAIX010000344 DATAIX010000000
VERSION     DATAIX010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48484"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..643
                     /locus_tag="VHM65_05125"
     CDS             266..643
                     /locus_tag="VHM65_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705121.1"
                     /translation="MVPFEAEASVLRESGLADEVVVADARSPLGLSQAVEAAGGPADL
                     TVVCVDVPGCEQPAILATAQGGTIIFFSMATSFAAAALGAEGVAADVTMLVGNGYTPG
                     HASYALDLIRANPGVRGLFERRL"
     gene            688..>1794
                     /locus_tag="VHM65_05130"
     CDS             688..>1794
                     /locus_tag="VHM65_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEX2705122.1"
                     /translation="MDRGLDRTEPGWWRRAVVYQIYPRSFADSDGDGVGDLRGIIGRL
                     DYLAALGVDVLWLSPVYRSPQDDNGYDISDYQDVDPLFGSLADLDELIASAHDRDLAV
                     IMDLVVNHTSDEHPWFAASRRSAADPKRDWYWWRPPRPGHRGGEPGAEPTPWGSVFSG
                     PAWEYDRATGEYYLHLFSRKQPDLNWENPEVREAIYAMMRWWLDRGVDGFRMDVINLI
                     SKPVGPDGSLPDEAVLAHGPRLHEFLAEMHAGVLAGRGQVYLAVGEMPGVSPEQAMAY
                     TDPARHELDMVFTFEHVALDHGPASKWDLRPLELPALKACMARWQQGLAKVGWNSLYW
                     DNHDQPRALSRWGDDAAYRVESAKTLLTTLHLHRG"
BASE COUNT          241 a          682 c          630 g          241 t
ORIGIN      
        1 ctcgcagccc gggacgtcga cgcagacgac ggtgaggtct gccggtcccc cggcggcctc
       61 gaccgcctgg gacagcccca gcggcgagcg ggcgtcggcg acgacgacct cgtcggccag
      121 ggcgctctcg cgcaggaccg aagcctcggc ctcgaacggc accaccccga taaggtgccc
      181 ggcgccgcag tcccgggcgg ccggcaagtc cgggtcgctg tcgctggccg ccgcccggga
      241 ctgcggcgcc ggtcacctca tcggggtggt gccgttcgag gccgaggctt cggtcctgcg
      301 cgagagcggc ctggccgacg aggtcgtcgt cgccgacgcc cgctcgccgc tcgggctgtc
      361 ccaggcggtc gaggccgccg ggggaccggc agacctcacc gtcgtctgcg tcgacgtccc
      421 aggctgcgag cagccggcga tcctcgcgac cgcccagggc ggcacgatca tcttcttctc
      481 gatggcgacg agcttcgccg ccgcggcgct cggcgccgag ggggtcgccg ccgatgtcac
      541 gatgctcgtc ggcaacggct acacgcccgg gcatgcgtca tacgcgctcg acctcatccg
      601 ggcgaacccc ggcgtccgcg ggctgttcga gcgccgcctc tgagcgccgc cgcacccgct
      661 cgccatgggg gcgctaccct cgcccccatg gaccggggac tggaccgcac cgagccgggg
      721 tggtggcggc gtgccgtcgt ctaccagatc tacccgcgca gcttcgccga ctccgacggc
      781 gacggcgtgg gcgacctgcg cggcatcatc gggcgcctcg actacctcgc cgccctcggc
      841 gtcgacgtcc tgtggctctc gccggtctac cgctcgccgc aggacgacaa cggctatgac
      901 atcagcgact accaggacgt cgacccgctc ttcggcagcc tcgcggatct cgacgagctc
      961 atcgcctcgg cccacgaccg agacctcgcc gtcatcatgg acctcgtcgt caaccacacc
     1021 tccgacgagc acccgtggtt cgcggcgagc cgccggtcgg ccgccgaccc caagcgtgac
     1081 tggtactggt ggcgcccgcc gcggcccggt caccggggcg gcgagccggg cgccgagccc
     1141 acgccgtggg ggtccgtctt ctcgggtccg gcctgggagt acgaccgcgc caccggcgag
     1201 tactatctgc atctcttctc gcgcaagcag cccgacctca actgggagaa ccccgaggtc
     1261 cgcgaggcca tctacgcgat gatgcgctgg tggctcgacc ggggcgtcga cggcttccgc
     1321 atggacgtca tcaacctcat ctccaagccg gtcgggccgg acgggtcgct ccccgacgag
     1381 gccgtgctcg cccacggccc gcggctgcac gagttcctcg ccgagatgca tgcgggggtg
     1441 ctggccggcc gcggccaggt ctacctcgcg gtgggggaga tgcccggggt gagcccggag
     1501 caggcgatgg cctacaccga cccggcgcgg cacgagctcg acatggtgtt caccttcgag
     1561 cacgtagccc tcgaccacgg tccggcgagc aagtgggacc tgcgcccgct ggagctgccc
     1621 gcgctcaagg cctgcatggc gcgctggcag caggggctcg cgaaggtcgg ctggaacagc
     1681 ctctactggg acaaccacga ccagccgagg gccctgtccc gctgggggga cgacgcggca
     1741 taccgggtgg agtcggccaa gacgctgctc acgacgctgc acctgcaccg cggg
//
LOCUS       DATAIX010000345         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_48499, whole genome shotgun sequence.
ACCESSION   DATAIX010000345 DATAIX010000000
VERSION     DATAIX010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48499"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VHM65_05135"
     CDS             <1..362
                     /locus_tag="VHM65_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX2705123.1"
                     /translation="QLTNRTLPLIGPDNVIYFRDVYDHAVHLAEEYDSYRELVSGALD
                     VYLSTVNNNLSLIMKRLTGITVVFAGLGAFAGIFGMSEAGAAFARTEAVGFWIVTAAI
                     VALSLTIFAVLRRIDWI"
     gene            complement(380..1084)
                     /locus_tag="VHM65_05140"
     CDS             complement(380..1084)
                     /locus_tag="VHM65_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705124.1"
                     /translation="MRSILGWVRGRLIPAVLTAAGVSLVGAGLLTYGTLAQATAPSPS
                     PLTSVAPSASLTLLPFPTPGASPRASQRPGTARVATRVIVPALRIDLPVVRPPNDPHA
                     FPYCDVAEYYTTLSQPGSPGTTYLFAHARDGMFLPLLVQSRNDNGKAMRGMLVQVYTS
                     DDKLFLYEITRVLRHQSELYSRPAEELVLQTSEGPRKGVPGYTGLVLVIVARPLSSGP
                     ATHAAAHPTAHPLVCE"
     gene            1244..1933
                     /locus_tag="VHM65_05145"
     CDS             1244..1933
                     /locus_tag="VHM65_05145"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX2705125.1"
                     /translation="MRTFIAVVIGLVVGVSAAIGLGVALVALTPTPDVRATPAPSAVV
                     QPGSPGPSPAPTEVVASATAVAIASGLAPTPVASRTVSPFPLVERPAPTLTAPQVGGG
                     VIDLATLRGKPVWLVFTGTYCPPCRDEYPVMNGFAARYADAGLVVLAIHVREGEQTVA
                     PFISSLNVVFPVGLDEDGSRARAWDAAALPVHYFIDAQGIVRDAALGGIGNDVMARGL
                     QAILPGVTVTP"
     gene            1930..>2167
                     /locus_tag="VHM65_05150"
     CDS             1930..>2167
                     /locus_tag="VHM65_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705126.1"
                     /translation="MRPGTTRGRRADRGGAGPASPATKQARDAPSTDDPEAGAVERTF
                     EVVQAVDAVRALAATTGPLLVVCDFDGTLAPISLD"
BASE COUNT          299 a          751 c          793 g          324 t
ORIGIN      
        1 accagctgac gaaccggacc ctgccgctca tcggacccga caacgtcatc tacttccgcg
       61 acgtctacga ccacgccgtt cacctggccg aggaatacga ctcctaccgg gagctggtct
      121 cgggcgccct cgacgtgtat ctgtcgacgg tcaacaacaa cctgtcgctg atcatgaaac
      181 ggctgaccgg gatcacggtg gtctttgccg ggctcggggc atttgccggg atcttcggga
      241 tgagcgaggc cggggccgcc ttcgcgcgca ccgaggccgt cggcttttgg attgttaccg
      301 cggcgatcgt cgccctgtcc ctgacgatct tcgccgtcct gcgcagaatc gactggatct
      361 gacggtcgcg gcgcggcccc tactcgcaca cgaggggatg cgcggtcggg tgggccgcgg
      421 cgtgggtggc cgggccggag ctgaggggcc gggccacgat gaccagcacc aggccggtat
      481 agccgggcac gcccttcctt gggccctccg aggtctgcag caccagctcc tcggcaggcc
      541 ggctgtagag ctccgactgg tgtcgcagga cgcgggtgat ctcgtagagg aagagcttgt
      601 cgtcgctggt gtagacctgg acgagcatcc cgcgcatcgc cttcccgttg tcgttccgag
      661 actggacgag cagcggcagg aacatgccgt cacgtgcgtg agcgaagagg tacgtggttc
      721 ccggggagcc gggctggctc agcgtcgtgt agtactcggc cacgtcgcag tacgggaagg
      781 cgtgcgggtc gttcggcggg cgcacaaccg gcaggtcgat cctcagggca gggacgatca
      841 cgcgtgtggc gacccgcgcc gtacccggac gctgcgaggc acgcggcgac gctcccgggg
      901 tcgggaacgg gagcagcgtc agggaggcgc tcggcgccac cgaggtcagc ggcgacgggc
      961 tcggtgcggt tgcctgcgcc agggtcccat acgtgagcaa tcccgccccg acgaggctga
     1021 cccccgccgc ggtgaggacg gccgggatga ggcggccgcg cacccagccg aggatcgatc
     1081 gcatggaagg gccatgctac cagcgacacc gggcgaggat gtcccgaggg ccgacgagcc
     1141 gagcgcgcgg cgtcgctgtc gccgagggcg ccgagggccg cagagctgag ccgggccgcg
     1201 ccgagcgcgc ggcatcgctg tcgacgcggt accctcgttt ccgatgcgca ccttcatcgc
     1261 ggtcgtcatc gggctcgtcg tgggggtcag cgccgcaatc ggccttgggg tcgctctcgt
     1321 cgcgctcacc ccgacgccgg atgtccgggc gaccccggcg ccttcggccg tggtgcagcc
     1381 gggatcgccc ggcccctcac ccgcaccgac cgaggtggtg gcgtccgcga ctgcggtcgc
     1441 gatcgcgagc ggtctcgcac cgacgcccgt tgcgagccga accgtttccc cgttcccgct
     1501 cgtggagcga ccggcaccga cgctgaccgc gccgcaggtc ggtggcggcg tgatcgacct
     1561 cgccacgctc cgagggaagc ccgtctggct ggtcttcacg ggcacctact gcccaccctg
     1621 ccgggacgag tacccggtca tgaatggctt cgcggcgcgc tacgcggacg cgggtctggt
     1681 cgtgctcgcg atccacgtcc gcgaagggga gcagaccgtg gcgcctttca tctcgtcgct
     1741 caacgtggtc tttccggtgg gcctcgacga ggacggctcg cgggcacggg cgtgggacgc
     1801 ggcggccctg ccggttcact acttcatcga cgcccagggg atcgtgcgcg acgccgccct
     1861 gggcggaatc ggcaacgacg tgatggcccg ggggctccag gcgatcctgc ccggtgtgac
     1921 cgtcacccct tgaggccggg gaccacccgc ggccgccggg ccgaccgggg tggggcaggt
     1981 ccggccagcc cggcgacgaa acaggcccgg gacgctcctt ccaccgacga tccggaggcg
     2041 ggcgccgtcg agcggacgtt tgaggtcgtg caggcggtgg acgccgtgcg cgcgctcgcc
     2101 gccacaaccg gcccgctgct ggtcgtgtgc gacttcgacg ggacgctggc gccgatctcc
     2161 ctcgacc
//
LOCUS       DATAIX010000346         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_186923, whole genome shotgun sequence.
ACCESSION   DATAIX010000346 DATAIX010000000
VERSION     DATAIX010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186923"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /gene="infB"
                     /locus_tag="VHM65_05155"
     CDS             complement(<1..1148)
                     /gene="infB"
                     /locus_tag="VHM65_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX2705127.1"
                     /translation="MQVPRGDGSTIIRVPRGASLTDFADKINANPASLVTVLFHLGEM
                     ATATQSLDEDTFTVLGQELGYDIQVVSPEDEERELLEAFHIDLEAEAEAETDDDLEAR
                     PPVVTVMGHVDHGKTKLLDAIRSAHVVESEAGGITQHIGAYQIVTDHEGVERAITFID
                     TPGHEAFTAMRARGAKVTDIAILVVAADDGVMPQTIEALNHAQAAGVPIVVAVNKIDK
                     EGANPDKIRQQLTEYNLVAEEYGGDTMFVEVSAKARTNIDGLLEAVLLTADAALDLRA
                     NPDKDARGVAIEAPLDKGRGPVATVLVQSGTLAVGDSIVAGSGHGRVRAMLDEHGNLL
                     DVALPSRPVQVLGLTSVPGAGDTFLVAPDDRTARQIAERREAASRAASL"
BASE COUNT          204 a          557 c          571 g          222 t
ORIGIN      
        1 agcgacgcgg cgcgcgaggc cgcttcgcga cgctcggcga tctgccgggc ggtgcggtcg
       61 tccggtgcca cgaggaacgt gtcaccggcc cccggcaccg aggtcaggcc gagcacctgg
      121 accggacgtg acggcagcgc cacgtcgagc aggttgccgt gctcgtcgag catggcacgc
      181 acccgaccgt gacccgagcc cgcgacgatc gagtcgccca cggccagggt tccagactgc
      241 acgaggacgg tcgccaccgg gccccggccc ttgtcgaggg gagcctcgat cgcaaccccg
      301 cgggcgtcct tgtcggggtt ggcacgcagg tcgagcgccg catcagcggt gaggagcacg
      361 gcctcgagca gcccgtcgat gttggtccgc gctttggccg acacctcgac gaacatcgtg
      421 tcgccgccat actcctcggc cacgaggttg tactcggtga gctgctggcg gatcttgtcg
      481 ggattggcgc cttccttgtc gatcttgttg accgcgacga cgatcggcac gcccgcggcc
      541 tgggcgtggt tgagtgcctc gatggtctgc ggcataacac cgtcgtcggc cgcgacgacg
      601 aggatggcga tgtcggtcac cttggcgcca cgagcacgca tggccgtgaa ggcctcatga
      661 cccggggtgt cgatgaaggt gatggcccgc tccacacctt cgtggtcggt gacgatctgg
      721 taggcaccga tgtgctgggt gatgccgccc gcctccgact cgacgacgtg ggccgaacgg
      781 atggcgtcaa ggagcttggt cttgccgtgg tcgacgtgac ccatgacggt gacgaccggc
      841 ggccgtgcct cgaggtcgtc gtcggtctcg gcctcggcct cggcctcgag gtcgatgtgg
      901 aaggcctcga gcagctcgcg ctcctcgtcc tccggcgaga cgacctggat gtcgtagccg
      961 agctcctggc cgagcaccgt gaaggtgtcc tcgtcgagcg actgggtcgc ggtcgccatc
     1021 tcgccgaggt ggaagagcac cgtgaccagc gacgcggggt tcgcgttgat cttgtcggcg
     1081 aagtcggtca gcgacgcacc gcgtggcacg cggatgatcg tcgacccgtc gccacgtggg
     1141 acctgcacgc caccgatggt cggggcctgc atctgctcga actcctggcg cttcgcccgc
     1201 ttggacttgc gcccccggac gggacggccg ccgccgcgcc cgaacgcgcc ctgcgtgccg
     1261 ccgcggccac cgggaccacc accgggacgt ccaccgaagc cgccgccacc gccggcgcca
     1321 cccgggctcc caccgccggg gccgccgggg ccgccggggc gtccggcgaa gccgccgcca
     1381 ccccccggac gggcgccggg acggccagga gccgcgccgg ggcgagtgcc cgggcgagcg
     1441 acggtcgccc ggtcgggcat catgcccggg ttcgggcggg gcccacccgg acgcggagca
     1501 cccggaccgg ggcgggcggc gccctgaccc ggtgcgcccg gtcgcgactg cggc
//
LOCUS       DATAIX010000347         5033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_48510, whole genome shotgun sequence.
ACCESSION   DATAIX010000347 DATAIX010000000
VERSION     DATAIX010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48510"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VHM65_05160"
     CDS             complement(<1..1122)
                     /locus_tag="VHM65_05160"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705128.1"
                     /translation="MDGGDLLMTRSVSASAQLARLGFAEPDRAAALLADPALAGLVDP
                     LDHRYRDGLPDALSAAADPDQALLGLVRLLESLARSPRRAGSLRLDTGGLLAAMRHPG
                     PGRDRLVAILGASSALIDHLVAHPEQWTAVTDARPRGAAELRTALTSAVGREQRGARP
                     AYDALRVAYRRELLGIAAIDLTSADPVAHLPRAAASLAWLAEAALEAALDIAREEYGP
                     GHEDCRLAVIGMGKTGGGELNYVSDVDVIFVAEPATGVAEDRALAVGTALATSLMKAC
                     SARTAEGTLWPIDPALRPEGKAGPLVRTVASHRAYYERWAKTWEFQALLKAWVSAGDM
                     EVGRAYKGVIAPMVWAAAGRERFVEDVQAMRRRVEAHVPA"
     gene            complement(1180..2517)
                     /gene="glnA"
                     /locus_tag="VHM65_05165"
     CDS             complement(1180..2517)
                     /gene="glnA"
                     /locus_tag="VHM65_05165"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX2705129.1"
                     /translation="MDRQQEFVLRTIEERDIRFVRLWFTDVLGTLKSVAVAPAELEGA
                     FAEGIGFDGSAIEGLSRVYEADMLVKPDPSTFQILPWRGENPGTARMFCDLLLPDGSP
                     SVADPRHVLQRALTKASTLGFTFYTHPEIEFFLFEGQARPGEGPQPVDNGGYFDHVPH
                     GAGHDFRRAAISMLENMGISVEFSHHEGAPGQNEIDLRYADALTTADNIMTFRAVVRE
                     VALEQGIYASFMPKPFREHPGSGMHTHLSLFEGDTNAFNEAGAPYQLSTTGRQFIAGL
                     LHHGSEITAVTNQWVNSYKRLWGGGEAPAYRTWGHNNRSALVRVPMYKPSKGNSTRVE
                     FRSIDSACNPYLAFAVLLMAGLKGIEGGYELPPEAEDDVWNLTDGERRAMGIEALPSS
                     LGAALRLMESSELVAETLGEHVFDFFLRNKRAEWQEYRQQITQFELDRYLSRP"
     gene            2574..3506
                     /gene="panB"
                     /locus_tag="VHM65_05170"
     CDS             2574..3506
                     /gene="panB"
                     /locus_tag="VHM65_05170"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX2705130.1"
                     /translation="MTDQPTSDQPTSPEQPTSPEQPSPYGSGPAASDTGSALAGAVAA
                     RKVRIPYLHRMKREGQRWAMLTAYDQYAAEIFDEAGIPVLLVGDSAGNNVYGYETTVP
                     VTVDELIPLVRAVTRSARRALVVADLPFGSYQGSPQQALATATRFMKEGLAHSVKLEG
                     GAGMAPAVELLVRSGIPVMAHIGFTPQSEHVLGGYRVQGRGDDGAAATLADALALEAA
                     GAFAIVIEMVPVSVAAAVTAALRIPTIGIGAGPHCDAQVLVWQDMAGLRGGRSPRFVK
                     RYADLRSVLRDAASAYAAEVASGAYPGLGHSFEG"
     gene            complement(3558..4286)
                     /locus_tag="VHM65_05175"
     CDS             complement(3558..4286)
                     /locus_tag="VHM65_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX2705131.1"
                     /translation="MADQAVERWHSERMQRDITVTRWGHYGTPVLVFPTAGGDAHEIE
                     RMGLVDRCRHLLDGGRVKIYSCDSVAGQALLRHEGSPEHRMWLFNQFHEAVAHEVVPA
                     IHTDTHSGGPVLVAGSSIGAFNSLAMLCRFPWLFSSAVCMSGSYRIEHLIGGTPNQDF
                     YFSSPLHFLPGLRGDQLDQARRRFVVLASGTGDYEDIGESWAAAGALGAKGIPNRVDD
                     WGPTYTHDWPTWWEMLPAYLAELA"
     gene            complement(4300..>5033)
                     /locus_tag="VHM65_05180"
     CDS             complement(4300..>5033)
                     /locus_tag="VHM65_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX2705132.1"
                     /translation="GELIDLTVASKALRRDHPVRVYLPARFQGSVRYPLLVVHDGTDY
                     LEYASAKPVLDNLIHRLDVAPLVVAFIPPGDRLVEYANHAPHARFVARELVPFLTERL
                     PLVDAPEGRCLMGSSFGGIASLSTAVRYPGLFDALLLQSASMVFTDIGTDHRGGSVFD
                     PVVRFVNGFRARPSRPVERIYQTCGIYEPLITANRSMVSTLRETGIEVRYAESRDGHN
                     WENWRDQLRGGLSWIFPGPQKFVYE"
BASE COUNT          741 a         1767 c         1811 g          714 t
ORIGIN      
        1 ggcgggaaca tgggcctcga cccggcggcg catcgcctgg acatcctcga cgaagcgctc
       61 ccgtcccgcg gcggcccaga ccatcggcgc gatgacgccc ttgtaggccc gccccacctc
      121 catgtcaccc gcggacaccc aggccttgag cagcgcctgg aactcccacg tcttggccca
      181 gcgctcgtag taggcgcggt ggctcgcgac ggtccgcacg agcggcccgg ccttgccctc
      241 ggggcgcagc gcggggtcga tcggccacag ggtgccctcg gccgtccttg cggagcaggc
      301 cttcatgagg gacgtcgcga gtgccgtgcc gacggccagc gcccggtcct cggccacacc
      361 ggtggcgggc tcggcgacga agatgacgtc gacatccgag acgtagttga gctcgccccc
      421 gcccgtcttg cccatcccga tcaccgcgag ccggcagtcc tcgtgccccg ggccgtactc
      481 ctcgcgggcg atgtccaggg ccgcctcgag agcggcctcc gcgagccagg ccagggacgc
      541 ggccgccctg ggcagatgcg cgaccgggtc ggccgaggtg aggtcgatgg ccgcgatccc
      601 gagcagctcg cgtcgatagg caacccgcaa cgcgtcgtat gccgggcgcg cgccacgctg
      661 ctcgcggccc accgccgacg tcagcgcggt ccgcagctcg gccgccccgc gagggcgtgc
      721 gtcggtgacg gccgtccact gctctggatg ggccacgaga tggtcgatga gggcgctcga
      781 cgccccgagg atggccacca gccggtcgcg ccccggtccc gggtggcgca tcgcggcgag
      841 cagcccgccg gtgtcgagcc ggagtgaccc ggcgcggcgg ggactgcggg ccagagactc
      901 gaggagccgg acgagcccga gcagggcctg gtcggggtcg gccgccgcgc tgagggcgtc
      961 aggcaagccg tcgcggtagc ggtggtcgag cgggtcgacc agcccggcca gcgccgggtc
     1021 ggcgagcagg gccgcggccc gatcgggctc ggcgaacccg agtcgggcga gctgggcgct
     1081 ggcgctgacg gaccgcgtca tcagcaggtc accgccgtcc acgaccgcgc caccgtcctc
     1141 acgcagtgca gcctacgacg cgaggagccg cgccctggct caggggcggg acaggtagcg
     1201 gtccagctcg aactgggtga tctgctggcg gtactcctgc cactcggccc gcttgttgcg
     1261 caggaagaag tcgaagacgt gctccccgag ggtctcggcg acgagctcgc tggactccat
     1321 cagccgcagg gcggccccga gcgagctcgg cagcgcctcg atgcccatgg cgcggcgttc
     1381 gccatcggtg aggttccaca cgtcgtcctc ggcctccggg ggcagctcgt agccgccctc
     1441 gatccccttg agaccggcca tgagcaggac cgcgaacgcg aggtaggggt tgcaggccga
     1501 gtcgatcgag cggaactcga cccgcgtcga gttgcccttg ctgggcttgt acatcggcac
     1561 ccggaccagg gccgagcggt tgttgtgacc ccacgtccgg tatgccggag cctcgccgcc
     1621 gccccagagc cgcttgtagg agttgaccca ctggttggtc acggcggtga tctccgaccc
     1681 gtggtgcagc aggcccgcga tgaactggcg cccggtcgtg ctgagctggt atggcgcccc
     1741 ggcttcgttg aaagcgttgg tgtctccctc gaagagggac aggtgggtgt gcatgcccga
     1801 gcccgggtgc tcccggaacg gcttgggcat gaacgaggcg tagatccctt gctcgagggc
     1861 cacctcacgg accaccgccc ggaaggtcat gatgttgtcc gcggtggtga gggcgtcggc
     1921 ataccgcagg tcgatctcgt tctgcccggg ggcgccctcg tggtggctga actcgaccga
     1981 gatgcccatg ttctcgagca tcgagatcgc cgcgcggcgg aagtcgtgcc cggcgccgtg
     2041 ggggacgtgg tcgaagtagc cgccgttgtc gaccggttgc ggtccctccc cgggccgggc
     2101 ctggccctcg aagaggaaga actcgatctc cgggtgggtg tagaaggtga agccgagcgt
     2161 cgacgccttg gtgagcgcgc gctgcaggac gtgacgcggg tcggcgacgc tcggcgagcc
     2221 gtcgggcagc agcaggtcgc agaacatccg ggcagtgcca gggttctcgc cgcgccaggg
     2281 caggatctgg aacgtcgacg ggtccggctt gaccagcatg tcggcctcgt agacccggga
     2341 caggccctcg atcgccgagc cgtcgaaccc gatcccctcg gcgaacgcgc cctcgagctc
     2401 tgccggggcc accgcgaccg acttgagcgt gccgagcacg tcggtgaacc acaaccggac
     2461 gaagcggatg tcgcgctcct cgatcgtgcg caggacgaac tcttgctgac ggtccatgcg
     2521 cgcatcctgc cgcacggaca tttcggcggt gttacgcgcc ctaccatggg gccatgaccg
     2581 atcagccgac atccgatcag ccgacatccc ctgagcagcc gacatcccct gagcagccca
     2641 gcccctacgg ttcgggtccg gcggcctccg acaccggctc agccctggcc ggggcggtgg
     2701 cggcccgcaa ggtccgcatc ccctacctgc accggatgaa gcgggagggg cagcgctggg
     2761 ccatgctcac ggcatacgac cagtacgctg cggagatctt cgacgaggcc ggtatccccg
     2821 tgctgctcgt cggggactcg gccggcaata acgtctacgg ctacgagacg accgtgcccg
     2881 tcaccgtcga cgagctcatc ccactcgtcc gggccgtgac gcgctcggcg cgccgggccc
     2941 tggtcgtggc tgatctgccc ttcgggtcct accagggcag cccgcagcag gcgctggcca
     3001 cggcgacgcg cttcatgaag gagggcctcg cccactcggt caagctcgag ggtggcgccg
     3061 ggatggcccc ggccgtcgag ctgctcgtgc gctccggcat cccggtcatg gcccacatcg
     3121 gcttcacccc gcagagcgag cacgtcctcg ggggctaccg cgtccagggc cggggcgacg
     3181 acggcgccgc cgcgacgctt gccgacgcgc tcgccctcga agcggcgggt gcgttcgcca
     3241 tcgtcatcga gatggtccct gtgtcggtgg ccgccgcggt gacggcagcc ctgcgcatcc
     3301 cgaccatcgg catcggcgcg ggtccgcact gcgacgccca ggtcctcgtc tggcaggaca
     3361 tggccgggct gcgcggtggg cgcagcccgc ggttcgtcaa gcggtatgcc gacctgcgct
     3421 cggtcctgcg cgacgccgcc agcgcgtatg ccgccgaggt cgcctccggc gcatacccgg
     3481 gcctcgggca ctccttcgag ggctgaggcc ggtccttcgg cgcggggtca tcaagcaccg
     3541 gctcaggcga gctcgggtca ggcgagctcg gccaggtagg cgggcagcat ctcccaccag
     3601 gtcggccagt cgtgggtgta cgtcggcccc cagtcgtcga cccggttcgg gatccccttg
     3661 gcgcccaggg cacccgccgc ggcccaggac tccccgatgt cctcgtagtc accggtcccc
     3721 gaggcgagga cgacgaaccg gcgccgcgcc tggtcgagct ggtcgccccg caggcccggg
     3781 aggaagtgca gcggcgagga gaagtagaag tcctggttcg gtgtcccgcc gatgaggtgc
     3841 tcgatccggt aggacccgct catgcacacg gccgaggaga acagccacgg aaagcggcag
     3901 agcatggcga gggagttgaa cgccccgatc gaggatcccg ccacgaggac cggcccgccg
     3961 gagtgggtgt cggtgtggat cgccggcacg acctcgtggg ccaccgcctc gtggaactgg
     4021 ttgaacagcc acatccggtg ttccggggac ccctcgtgcc gtaagagcgc ctggccggcc
     4081 acgctgtcac acgagtagat cttgacccgg cccccgtcga gcagatgccg gcatcggtcg
     4141 accaggccca tccgctcgat ctcgtgcgca tcgcccccgg cggtggggaa gacgaggacg
     4201 ggcgtgccgt agtggcccca ccgggtcacc gtgatgtccc gctgcatgcg ctcggagtgc
     4261 cagcgctcga cggcctggtc ggccatgtcg tcctgcttcc tactcgtaga cgaacttctg
     4321 gggcccgggg aagatccacg acagcccacc ccggagctgg tcccgccagt tctcccagtt
     4381 gtgcccgtcg cgcgactcgg cataccggac ctcgatcccc gtctcgcgca aggtcgagac
     4441 catggaccgg ttggcggtga tgagcggctc gtagatgccg caggtctggt agatccgctc
     4501 caccggccgc gagggccgcg cgcggaatcc gttgacgaac ctgacgacag ggtcgaagac
     4561 cgacccgccg cggtggtcgg tgccgatgtc ggtgaacacc atcgaagccg actgcagaag
     4621 cagcgcgtcg aacagccctg ggtaacggac cgccgtggag agcgacgcga tccccccgaa
     4681 gctcgaaccc atgaggcatc gtccctcagg ggcgtcgacc aaaggaagcc gctcggtgag
     4741 gaagggcacg agctcgcggg ccacgaaccg cgcatgcggc gcgtggttgg cgtactcgac
     4801 gagccggtcg cccggcggga tgaacgccac gacgagcggt gccacgtcga ggcgatggat
     4861 gaggttgtcg aggacgggct tggccgaggc gtactcgagg tagtcggtcc cgtcatggac
     4921 gacgagcagc gggtagcgca ccgacccctg gaagcgcgcc ggcaggtaga ccctgaccgg
     4981 gtggtcccgg cgcagcgcct tgctcgcgac ggtgaggtcg atgagctccc cgg
//
LOCUS       DATAIX010000348         1838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_78504, whole genome shotgun sequence.
ACCESSION   DATAIX010000348 DATAIX010000000
VERSION     DATAIX010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78504"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..995)
                     /locus_tag="VHM65_05185"
     CDS             complement(318..995)
                     /locus_tag="VHM65_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX2705133.1"
                     /translation="MDLDVVRSAPLFAALDEEDARALLAQMAPFRMERGDILFNEGDR
                     GDTLFVIAEGKVKLGRTSSDGRENLVAVLGPGEMFGELSLFDPGPRTMTATAVAETQL
                     VALGNDSLTEILAGRPEVSKSLLAALAQRLRRTNEHLADLVFTDVPGRVAKALLDLSH
                     RFGRPVEGGLLVAHDLTQEELAQLVGASRETVNKALADFATRGWLKLEARAVLLIDVD
                     RLTRRAR"
     gene            1161..1685
                     /locus_tag="VHM65_05190"
     CDS             1161..1685
                     /locus_tag="VHM65_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705134.1"
                     /translation="MVHACESPVALTRRARKMYRVLAERYPYARCELDFTTPLELLVA
                     TILSAQTTDVRVNLVTPVLFEKYRTATDYAGADRAEVESVIASTGFFRAKTVSLLKLG
                     AILVERYDGQVPSRLADLVSLPGSAARRPMSSSAMRSASPASRSTRTSGGSRAGSGGP
                     RRRIRSRSSTPSVR"
BASE COUNT          264 a          657 c          654 g          263 t
ORIGIN      
        1 cgctgagccc gcagagcccg actacgccga ctcaggcgct ggccccgatg gccggtcgag
       61 ataggccagc tgcgcccgca ccgacagctc ggccgccggc cacacctcca gcgggacgtc
      121 ccagtagacc cgctcgacca ggtccgcgag gcccgggctg ccggggcccg cgacgtgcag
      181 gacgtgctcg agcgggtcta ctgggacgtc cctcgcgagg tgtggccggc cgccgagctg
      241 tcggtgcggg cgcagctggc ctatctcgac ggacctttcg gggccagcgg ctcagccgct
      301 cagcgggctc agcgggctca gcgcgcgcgg cgggtgagcc ggtcgacgtc gatgaggagc
      361 acggcgcggg cttcgagctt gagccagccg cgggtcgcga agtcggccag cgccttgttg
      421 accgtctcgc gggaggcccc gaccagctgg gcgagctcct cctgggtgag gtcatgggcg
      481 acgaggaggc cgccctcgac gggccgcccg aagcggtgcg acaggtcgag caacgccttg
      541 gcgacccggc cggggacgtc ggtgaagacg aggtcggcga ggtgctcgtt ggtgcggcgc
      601 aggcgctggg cgagggcggc cagcagggac ttggacacct cgggacgccc ggcgaggatc
      661 tcggtgagcg agtcgttgcc gagcgcgacg agctgggtct cggcgacggc ggtggccgtc
      721 atcgtgcgcg ggcccgggtc gaacaggctc agctcaccga acatctcgcc tgggccgaga
      781 accgccacga ggttctcccg gccgtcactg ctcgtccgcc cgagcttgac cttgccctcg
      841 gcgatgacga acaaggtgtc gccgcggtcg ccctcgttga agaggatgtc gccgcgctcc
      901 atgcggaagg gcgccatctg ggccaggagc gcccgggcgt cctcctcgtc gagggcggcg
      961 aacagcgggg cgcttcgcac gacgtcgaga tccacgggct cagtgtgcca tgaggtcggc
     1021 ccgcaggctc ggcccgcaac acagttcccg caacacagtt cccgcaacac agtccccggc
     1081 aacacagtcc ccggcaacac tgtccccggc aacactgtcc ccggcaacac tgtcacggct
     1141 gcgccgtcta cgctgtcccg gtggtgcatg cctgcgagag ccccgttgcg ctgacccggc
     1201 gcgcccgcaa gatgtaccgg gtcctggccg agcgctaccc ctacgcccgc tgcgagctcg
     1261 acttcacgac cccgctggag ctcctcgtcg cgaccatcct gtcggcccag acgaccgacg
     1321 tcagggtcaa cctggtgact ccggtcttgt tcgagaagta ccggacggca acggattacg
     1381 cgggcgcaga ccgggccgag gtcgagtcgg tcatcgcctc gaccggcttc ttccgggcca
     1441 agaccgtctc actgctcaag ctgggcgcga tcctcgtcga gcggtatgac ggtcaggtcc
     1501 cctcgcgcct ggccgacctc gtcagcctgc ccgggtcggc cgcaagacgg ccaatgtcgt
     1561 cctcggcaat gcgttcggcg tccccggcat cacggtcgac acgcacttcg ggcggctctc
     1621 gcgccggttc gggtggaccg aggaggagga tccggtcaag gtcgagcacg ccgtcggtgc
     1681 gctgatcccg cgcaaggact ggacaatgct cagccacgtc gtcatcttcc acgggcgccg
     1741 gacgtgccat tcccgccgtc cggcctgcgg cgcgtgcccg gtggcgcgct ggtgcccgtc
     1801 gtacggcatc ggggagaccg acccggacca ggctgcga
//
LOCUS       DATAIX010000349         2843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_108198, whole genome shotgun sequence.
ACCESSION   DATAIX010000349 DATAIX010000000
VERSION     DATAIX010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108198"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VHM65_05195"
     CDS             complement(<1..789)
                     /locus_tag="VHM65_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX2705135.1"
                     /translation="MTLRLFDTATRGLRDFVPQTPGKAGIYICGLTTQGLPHIGHLRF
                     TVAFDVLRRWLETGHGYDVTLIRNVTDIDDKILAKSAHAGVDWFALSYANERATSAAL
                     DLLGVRPATYEPRATGHVPEMVELMDALVERGHAYAADDGSGDVYFDVTSWPRYGELS
                     HQAIEDMAPSEDADPRGKRDPRDFALWKGRKAHEPTSASWPTPYGTGRPGWHLECSAM
                     ARKYLGDSFDIHGGGIDLRFPHHENEQAQSRAAGLGFARYWLHNA"
     gene            complement(1075..1737)
                     /locus_tag="VHM65_05200"
     CDS             complement(1075..1737)
                     /locus_tag="VHM65_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2705136.1"
                     /translation="MTTATRVGVASPGGVRVEHLVISGTFSLDGGTWEVDNNVWVVGD
                     DSECVVIDAPHEGAPILSAVGGRTLKGVLCTHAHDDHITAVGELCRATGAPSYLNPDD
                     RVLWDRMYDVAPDHELADGDVLTVGDVGLHVLHTPGHAPGATCFYAPGLGVVFTGDTL
                     FRGGPGATGRSFSDFPEIVESITSRLLILPEDTVVHTGHGDSTTIGAEKPHVQEWLDR
                     GH"
     gene            complement(1734..2819)
                     /locus_tag="VHM65_05205"
     CDS             complement(1734..2819)
                     /locus_tag="VHM65_05205"
                     /EC_number="1.1.1.306"
                     /inference="COORDINATES: protein motif:HMM:TIGR03451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050607 - mycothiol-dependent formaldehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010127 - mycothiol-dependent
                     detoxification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)mycothiol dehydrogenase"
                     /protein_id="HEX2705137.1"
                     /translation="MPSTVTGVIARAKGAPVELVDIVVPDPGPGEAVVTVEACGVCHT
                     DLHYRDGGINDAFPFLLGHEAAGTVEAVGEGVSDLAPGDFVILNWRAVCGQCRACRRG
                     KPWYCFATHNATQKMTLTDGTELTPALGIGAFVEKTLVAAGQCTKVDRDASAAAVGLL
                     GCGVMAGFGAAVNTGGAGRGDSVAVIGCGGVGNAAIAGAHVVGATRIIAVDLDDRKLE
                     LARSFGATHTVNSGREDAVEKIREYTGGHGADVVVEAVGRPETYEQAFYARDLAGTVV
                     LVGVPTPERQVTLPMIDIFGRGGALKSSWYGDCLPSRDFPMLVDLYRQGRLDLDAFVS
                     ETIGIGDVEAAFEKMHHGDVLRSVVVL"
BASE COUNT          412 a         1000 c         1002 g          429 t
ORIGIN      
        1 ggcgttgtgc agccagtagc gggcgaaccc cagcccggcc gcccgcgact gggcctgctc
       61 gttctcgtgg tgcgggaagc gcaggtcgat gccgccgccg tggatgtcga agctgtcccc
      121 gaggtacttg cgagccatcg ccgagcactc gaggtgccag cccggccgcc cggtgccata
      181 cggcgtgggc cagctggccg acgtgggctc gtgcgcctta cgccccttcc acagggcgaa
      241 gtcgcggggg tcgcgcttgc cgcgcgggtc ggcgtcctcg gagggggcca tgtcctcgat
      301 cgcctggtgg ctcagctcgc catagcgcgg ccaggacgtg acgtcgaagt agacatcgcc
      361 actgccgtcg tccgccgcat aggcgtgccc gcgctccacg agggcgtcca tgagctcgac
      421 catctcgggc acgtggccgg tcgcccgcgg ctcgtacgtg gccggccgga ccccgagcag
      481 gtccagcgcc gccgacgtgg cccgctcgtt ggcgtaggac agggcgaacc agtcgactcc
      541 ggcatgtgcc gacttggcga ggatcttgtc gtcgatatcc gtgacgttgc ggatcagcgt
      601 gacgtcatac ccatgccccg tctcgagcca gcgccggagc acgtcgaagg cgacggtgaa
      661 gcgcaggtgg ccgatgtggg gaaggccctg ggtcgtgagc ccgcagatgt agatgcccgc
      721 cttgccgggg gtctgcggga cgaagtcgcg cagaccacgc gtcgcggtgt cgaacaggcg
      781 caaggtcact cgccacaggg tacccgggcg gcccaccccc acgcgttcgc gtgaaggtat
      841 cgggggtcga ctcgacggtc tatgacacga cctcgatcct ggcaacgatc gagcacggct
      901 tcgggctggc accgctcgcc gcgcgcgacg cccgggtcaa cgacctgcgc aacgcgatcc
      961 agacgggcct gctatggcta ggcacggaga gccggcccgg cttgcgtatg acggcgccgg
     1021 accgcctcgc ctcagcggtg gaggtagctt cgcagaccgg ccagcaccac ggcgtcagtg
     1081 tcctcggtca agccactcct gcacgtgcgg cttctccgcg ccgatcgtcg tcgagtcgcc
     1141 gtgaccggtg tggaccacgg tgtcctcggg caggatcagc aggcggctgg tgatcgactc
     1201 gacgatctcc gggaagtcgc tgaagctgcg ccccgtcgct cccggtccgc ccctgaagag
     1261 cgtgtccccg gtgaaaacca ccccgaggcc cggggcgtag aagcacgtcg cccccggcgc
     1321 gtgccccggc gtgtgcagca cgtgcaggcc gacatcgccc acggtgagca cgtcgccgtc
     1381 ggccagctcg tggtccggtg cgacgtcata catccggtcc cacaggaccc ggtcatccgg
     1441 attgaggtat gacggtgcgc cggtggcgcg gcacagctca ccgaccgcgg tgatgtggtc
     1501 gtcgtgcgcg tgggtgcaca ggaccccctt gagcgtgcgg ccgccgaccg ccgagaggat
     1561 cggggcgccc tcatggggcg cgtcgatgac gacgcactcg ctgtcgtcac ccacgaccca
     1621 gacgttgttg tccacctccc acgtgccgcc gtcgaggctg aatgtgcctg agatgacgag
     1681 gtgttcgacg cggacgccgc cgggcgaggc gacgccgacc cgggtcgccg tcgtcacagg
     1741 acgaccaccg agcgcaggac gtcgccgtgg tgcatcttct cgaacgccgc ctcgacgtcg
     1801 ccgatcccga tcgtctcgga gacgaacgcg tcgaggtcga gccggccctg gcggtagagg
     1861 tcgacgagca tcgggaagtc acgcgagggg aggcagtcgc cataccagct ggacttgagc
     1921 gcgcccccac ggccgaagat gtcgatcatc ggcaaggtga cctgccgctc gggtgtgggg
     1981 acgccgacga ggaccaccgt gccagccaga tccctcgcgt agaacgcctg ttcgtacgtc
     2041 tcgggccgcc cgaccgcctc gacgacgaca tcggcgccgt gcccgccggt gtactcgcgg
     2101 atcttctcga ctgcgtcctc acggccgctg ttcaccgtgt gggtcgcgcc gaaggacctg
     2161 gccagctcga gcttgcggtc gtcgaggtcg acggcgatga tcctcgttgc cccgacgaca
     2221 tgggcgcccg cgatggccgc gttgccgacg ccgccgcagc cgatgaccgc gaccgagtca
     2281 ccgcgcccgg caccaccggt gttgaccgcg gcaccgaagc ccgccatgac gccacagccg
     2341 agcagcccta ccgcggcggc cgaggcatcc cggtcgacct tggtgcactg gccggcggcc
     2401 acgagggtct tctcgacgaa ggcgccgatg cccagtgccg gcgtgagctc ggtcccgtcg
     2461 gtgagggtca tcttctgggt ggcgttgtgg gtggcgaagc agtaccaggg cttgcccctc
     2521 ctgcacgcgc ggcactgccc acagaccgcc ctccagttga ggatgacgaa gtcacccggg
     2581 gcgaggtcgc tgaccccctc gccgacggcc tcgaccgtgc ccgcggcctc gtgcccgagc
     2641 aggaacggga atgcgtcgtt gatcccgccg tcgcggtagt gcaggtcggt gtggcagacg
     2701 ccgcaggcct ctaccgtgac gaccgcctca cccggccccg ggtctgggac gacgatgtcg
     2761 acgagctcga cgggcgctcc tttggcgcgg gcgatgactc ccgtgacggt gctgggcatg
     2821 accggtcctt cctgcgctgt cgg
//
LOCUS       DATAIX010000350         2884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_108204, whole genome shotgun sequence.
ACCESSION   DATAIX010000350 DATAIX010000000
VERSION     DATAIX010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108204"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VHM65_05210"
     CDS             <1..583
                     /locus_tag="VHM65_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX2705138.1"
                     /translation="IGIVSLGGRAGTGKSALALCAGLETVMERRQHRKVVVFRPLYAV
                     GGQELGYLPGTEAEKMGPWGQAVFDTLGAVVSTEVVEEILDRGMLDVLPLTHIRGRSL
                     HDAFVIVDEAQSLERNVLLTVLSRIGQNSKVVLTHDVAQRDNLRVGRFDGVAAVIEAL
                     KGHPLFAHITLTRSERSPIAALVTELLEGLEVG"
     gene            879..1475
                     /locus_tag="VHM65_05215"
     CDS             879..1475
                     /locus_tag="VHM65_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705139.1"
                     /translation="MALLRSRRPSASGVALVALLGTAGWGLSSSPPSAAETQDVSTQV
                     SAPTRAPEFTEGRIVDLQEIEQAEAERVILADRATAARAARDAARKAIIAHAKTDPQS
                     AARAMLGDFGWNARQMPCLVTLWHGESAWHWNADNRSSSAYGIPQALPGSKMASAGAD
                     WRTNPVTQIRWGMAYITRVYGSPCSALSRWSSRYPHWY"
     gene            complement(1667..2665)
                     /gene="coaA"
                     /locus_tag="VHM65_05220"
     CDS             complement(1667..2665)
                     /gene="coaA"
                     /locus_tag="VHM65_05220"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEX2705140.1"
                     /translation="MSSLPMVPPPVPDTRVVERRGGEPSPYVDLDRDAWSRLRENHPL
                     NLTEHDLTRLRGRSDPLDLTEIEAVYLPLSRLLNFYVAATSGLHRITTDFLGEGPAKT
                     PFIIGVAGSVAVGKSTTSRILRELLRRWPGTPEVQLITTDGFLLPNAELSRRGLLARK
                     GFPESYDRRALLRFLGDIKAGQAEVSAPVYSHLSYDIVADERVVVRRPDVLIVEGLNV
                     LQTPPLRADGATALAVSDYFDFSVYVDAQVRDIRRWYVERFLRLRETAFANRESYFHR
                     YAGLTDAQARTTALQIWAEINGPNLVQNISPTRNRATLVLTKGSDHHVERVRLRKL"
BASE COUNT          424 a          956 c         1063 g          441 t
ORIGIN      
        1 tatcgggatc gtctcgctcg gcggccgcgc ggggaccggc aagtccgccc tcgcgctgtg
       61 tgccgggctc gagacggtca tggagcgccg ccagcaccgc aaggtggtcg tcttccggcc
      121 gctgtatgcc gtcggcggcc aggagctggg ctaccttcct ggcaccgagg ccgagaagat
      181 gggcccgtgg ggacaagccg tgttcgacac tctcggagcc gtcgtctcga ccgaggtggt
      241 cgaggagatc ctcgaccggg gcatgctcga cgtgctgccg ctgacccaca tccggggccg
      301 ttcgctgcac gacgcgttcg tcatcgtcga cgaggcacag tcccttgagc gcaacgtcct
      361 gctgactgtg ttgtcgcgca tcggccagaa ctccaaggtc gtgctcaccc atgacgtggc
      421 ccagcgggac aacctgcggg tcgggcgctt cgacggggtg gcggccgtca tcgaagcgct
      481 caagggccat ccgctctttg cccatatcac cttgacccgc tccgaacgca gcccgatcgc
      541 ggcgctggtc accgagctgc tcgagggcct tgaggtcggc tgacggctcg tcccgccgga
      601 cggtccctgg cgcaggcctg ggaccggtgg tcgagggacc ctgaccgcgg ccgggatttg
      661 gcatcgggcc ggcccgtgtg caaccgtgga attacatcac ggattggtaa cggcctcgtc
      721 gaggtgaacg ggccggtcac catggaggtc cacacgtggg gtcgaagtac gcgggtcagc
      781 atcgagggtc agccaccacg ggtcgtgcgc cgatccgcca ttcgggcgcc ggcgccgagc
      841 ccagtgccgg gtcctgtcag cccagcacga cggcgggagt ggctctgttg cgctcgcgcc
      901 gtccgagcgc gtcgggggtc gcgctcgtcg ccttgctcgg caccgccgga tggggcctgt
      961 cctcgtcgcc gccgagcgcc gccgagactc aggacgtctc gacgcaggtc agtgcgccaa
     1021 cccgtgcgcc ggagttcacc gagggacgca tcgtcgacct ccaggagatc gagcaggccg
     1081 aggccgagcg ggtcatcctc gccgaccggg ctaccgcagc gagggcagcc cgcgacgcgg
     1141 cgcgcaaggc gatcatcgcg cacgccaaga ccgaccctca gtcggcggcc agggcgatgc
     1201 tcggtgactt cggctggaac gcgcggcaga tgccgtgcct ggtcaccttg tggcacggcg
     1261 agagtgcctg gcactggaac gccgacaacc gctcgtcgag cgcctatggg atcccgcagg
     1321 cccttcccgg ttccaagatg gccagcgcgg gggcagactg gcggaccaac ccggtgaccc
     1381 agatccgctg ggggatggcg tacatcacga gggtctacgg gtcgccgtgc tcggctctct
     1441 cgcgatggag ctcgcgctac ccccactggt actgaccgca tcagggccac ggccccggcg
     1501 ggggctgccc ggctgggggc ccgcaccggg cttgggtcac gacggcacgg ttgcagccgt
     1561 cgtgtcgaca ccccacgtgt gcgccgcccc cggcgccgag ctcgtcggtc ggctcctcga
     1621 tcacgtccgg cttcttcggc ggcagccgtc gctgagactc gagagctcag agcttgcgga
     1681 gccggacccg ctcgacatgg tggtcggacc ccttggtgag cacaagggtg gcccggttgc
     1741 gtgtcggtga gatgttctga acgaggttgg gcccgttgat ctcggcccag atctgcagcg
     1801 ccgttgtccg cgcctgcgcg tcggtcagcc cggcataccg gtggaagtag gactcccggt
     1861 tcgcgaacgc cgtctcgcgc aggcgaagga accgctcgac ataccagcgc cggatgtcgc
     1921 gcacctgtgc gtcgacatac acggagaagt cgaagtagtc gctgacggcg agggcggtgg
     1981 cgccgtcagc gcgcaggggt ggggtctgca gcacgttgag gccctcgacg atgaggacat
     2041 cggggcgccg gacgacgacg cgctcgtcgg cgacgatgtc gtagctgagg tgcgagtaga
     2101 ccggcgcgct cacctcggcc tggccggcct tgatgtcacc gaggaaccgc agcagcgccc
     2161 ggcggtcgta ggactcggga aaccccttgc gcgccagcag tcctcgacga gacagctcgg
     2221 cgttggggag gaggaagcca tcggtcgtga tgagctggac ctcgggcgtg ccgggccagc
     2281 ggcgcagcag ctcgcgcagg atgcgcgacg tcgtcgactt gcccaccgcg accgagccgg
     2341 cgacgccgat gatgaagggt gtcttggccg gcccttcgcc gaggaagtcc gtcgtgatgc
     2401 ggtgcagccc agaggtcgcg gcgacgtaga agttgagcag ccgcgacaac ggcaggtaga
     2461 cggcctcgat ctcggtcagg tccagggggt cgctccgtcc ccgcaaccgg gtgaggtcgt
     2521 gctcggtgag gttgagcggg tggttctcgc gcaggcgcga ccaggcatca cggtcgaggt
     2581 cgacgtaggg cgagggctca ccgccgcgcc gctccaccac ccgggtgtca gggacgggcg
     2641 gcggcaccat cggcagcgaa gacaccgggc cattgtggcc ccttcggccc gggcgcgtcc
     2701 gcggcggatc gccaggagcc gggaataagg cccgttaggc tggtcaccat gtgtggaatc
     2761 gtggggtacg tgggcccgac tgcagacggc cgggctctca atgtcgtcat ggagggcctg
     2821 gcccggctcg agtaccgtgg ctacgactcg gccggggtgg ccatggttgc ggcgggccag
     2881 gtgg
//
LOCUS       DATAIX010000351         1621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_78818, whole genome shotgun sequence.
ACCESSION   DATAIX010000351 DATAIX010000000
VERSION     DATAIX010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78818"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..344)
                     /locus_tag="VHM65_05225"
     CDS             complement(114..344)
                     /locus_tag="VHM65_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705141.1"
                     /translation="MQIDCDRCPVRGTHCESCFVTALARLPVLALDPQDQALDPAEQR
                     AVDLFVAAGLVAADEAAGLRARREPWRQASAV"
     gene            complement(663..1466)
                     /locus_tag="VHM65_05230"
     CDS             complement(663..1466)
                     /locus_tag="VHM65_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2705142.1"
                     /translation="MARYRQWDVNVGLVLGREGAVVIDTRSSGIQGAEIQDDIRALGA
                     GIRVRHVVNTHVHFDHTLGNCAFEGAAVYAHENVGRTIERDSAAVMELFRADPRDAPE
                     YGYTAADVVGVLATEVRVPDVTFDSVVKVDLGNRRVVMTHTGRGHTDGDVVVTVPDAD
                     VAFFGDLVEESAHPSLGEDCWPLEWAPTLDAHAVGLGRGSVVVPGHGLPVGSSFVLRQ
                     REDLAVVAAVIAERRAAGLTLEQASRQPDDRLPYPLEWLTTAFARAWGQ"
BASE COUNT          264 a          610 c          538 g          209 t
ORIGIN      
        1 cgctgactcg tcggagtcgg ccgggtcgtg gccacggacg agcagcacgc agccactgtc
       61 atcgaccagg acgacgcggg ccgcccgccg gtagaggtgc ccggacgggc ccgtcatact
      121 gccgaagcct gtcgccaggg ctctcggcga gcccgcagcc cggccgcttc gtcggctgcg
      181 acgaggcccg ccgcgacgaa caggtcgacg gcccgctgct cggctgggtc cagagcctga
      241 tcctgcgggt ccagcgccag caccggcagc cgtgcgagcg ccgtcacgaa gcagctctcg
      301 caatgggtgc cgcggaccgg gcaccggtcg cagtcgatct gcatgtgcgt catctcgtgc
      361 cgcctcctgt ggcgtcttgc gctggtctgc gaggagcccg gtcgacccgc ccgcccaggg
      421 ctgtcctggt acccaccacg ctaggaccga gcaccgacat accacccgca gaccggtcca
      481 gaacggcccg tcgatgtgcc gcagaacgcc accccgcaca tgaccccgta aggaaccgag
      541 gccgaagtga ccggccacca ccggccagac caacctcagt ccaggccccc ctcgacagcg
      601 aggccgaggt gaccggtcac taccggccgg aggcaaacaa cgcctgaaag cgcgaggcct
      661 gctcactgcc cccaggcgcg ggcgaaggcc gtcgtcagcc actcgagcgg gtagggcagc
      721 cggtcgtccg gctggcgact cgcctgttcg agcgtcagcc ccgccgcccg gcgctcggcg
      781 atcaccgccg ccaccacggc gaggtcctcg cgctggcgca ggacgaacga cgagcccacc
      841 ggcagcccgt gcccgggcac gaccacactc ccccggccga gacccaccgc atgggcgtca
      901 agcgtcggcg cccactccag cggccagcag tcctcgccca gcgaggggtg ggccgactcc
      961 tcgacgagat ccccgaagaa cgccacatct gcatccggga ccgtcacgac gacgtcgccg
     1021 tcggtgtgtc cgcgaccggt atgagtcatg accacgcggc ggttgccgag gtcgaccttg
     1081 acgaccgagt cgaacgtcac gtccggcacg cgcacctccg tcgcgagcac accgaccaca
     1141 tccgcggcgg tgtagccgta ctccggcgca tcccgaggat ctgcgcggaa cagctccatg
     1201 accgcggcag agtcgcgctc gatcgtgcgc ccgacgttct cgtgggcgta gaccgcggcg
     1261 ccctcgaatg cgcagttccc caaggtgtgg tcgaagtgca catgggtgtt gacgacgtgc
     1321 ctgacccgga tccccgcgcc gagcgcacgg atgtcgtcct ggatctcggc cccctggatg
     1381 ccgctcgacc gggtgtcgat gacgacggct ccttcgcgcc ccagcacgag cccgacattg
     1441 acgtcccact gccggtagcg agccacgaac acccggtcgg cgactacgcg gccgcggtcg
     1501 caagcgagat ccagtggttg gtgacggccg cgcgcgaagg gtcaggacaa cgcgagggta
     1561 tgccggtacc gccggcgtgt ggcgacccgt tctttgccga gctgttcgac tacggagccg
     1621 a
//
LOCUS       DATAIX010000352         2720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_48818, whole genome shotgun sequence.
ACCESSION   DATAIX010000352 DATAIX010000000
VERSION     DATAIX010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48818"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..798
                     /locus_tag="VHM65_05235"
     CDS             52..798
                     /locus_tag="VHM65_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX2705143.1"
                     /translation="MPESCEPAPRRGSEIARFLRAALVEAVPRDRQHSAAEVRHRRVV
                     SALTLVVGAALLAISINLTPGDGRFYATTLALAAVWTIGALASGPLHAGWANTRAGTR
                     HTRPVVQPLAVGLFFVGLFALGALAVARTDAPRQAIEAVLDHARYASLPVVAAITVVN
                     GLAEELFFRGSVYAAIGPRRPVLWSTVLYAVTTLASGNLMLVLAALILGAVAGLQRRV
                     TGGVLGPMITHVTWSMSMLLILPALLHAAG"
     gene            1312..2310
                     /locus_tag="VHM65_05240"
     CDS             1312..2310
                     /locus_tag="VHM65_05240"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX2705144.1"
                     /translation="MTDAPSGLVLVTGATGYIGSRLVPALLDAGWAVRVLTRSAAGVA
                     DRGWASRVEVAQGDAADPAVLAEACAGVQVAYYLLHSMDGRPDFERRDRRMALGFARA
                     AREAGVGRIVYLSGLHPEGVRLSSHLESRTEVAEILLASGVPTTVLQAAVILGSGSAS
                     FEMLRHLTRRLPAMVAPKWLTNRIQPIAVRDVLTYLVASAQMPSQVSRTFDIGGPDVL
                     TYKQMIGRFAQIDGLRARPIMTVPVLTPWLASHWVGVVTPVPASIAKPLVGSLIHEVV
                     CKEHDIAGLVPDPPGGLLGFDEAVRAALAGEESTRPEPGTVDPAWLTSADPDWAGS"
     gene            2409..>2720
                     /locus_tag="VHM65_05245"
     CDS             2409..>2720
                     /locus_tag="VHM65_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705145.1"
                     /translation="MNPRSVRPLIAAVAALGAVLAGPALAVAPSQAAQGPALGPALGS
                     ASGREAAPGDVRVIRSLTSLTATHRWFEQTYGGLPVVDGFYVARTDIRTGARSVTDGR
                     QG"
BASE COUNT          312 a         1048 c          950 g          410 t
ORIGIN      
        1 gttgccgggg cgctctcggg tcgggccggt cctcgcccgt acagtgttcg ggtgcctgag
       61 tcctgcgagc cggcgccgcg gcggggaagc gagatcgccc gcttcctgcg cgccgcgctg
      121 gtcgaggcgg tgccccggga ccgtcagcac agcgccgccg aggtccgcca ccggcgcgtg
      181 gtctcggcgc tgaccctggt ggtcggcgcc gccctgctcg ccatctcgat caacctcacg
      241 ccgggcgacg ggcgcttcta cgcgacgacg ctcgcgctcg ccgcggtctg gaccatcggt
      301 gccctggcct cgggtccgct gcacgcgggg tgggccaaca cccgggccgg cacgcgccat
      361 acccggccgg tcgtccagcc gctcgcggtg ggcctgttct tcgtcgggct cttcgccctc
      421 ggagcgctcg ccgtggcacg cacggacgcg ccacggcagg ccatcgaagc ggtcctcgac
      481 cacgcacgct atgcctcgct cccggtcgtc gccgcgatca ccgtcgtcaa cgggctggcc
      541 gaggagctgt tcttccgcgg ctccgtctac gcggcgatcg ggccgcgccg gcccgtcctg
      601 tggtccacgg tcctgtatgc cgtcacgacc ctggccagcg gcaatctcat gctcgtcctc
      661 gcggcgctca tcctcggcgc cgtggccggg ctgcagcggc gggtcaccgg gggagtgctc
      721 gggccgatga tcacccacgt cacgtggtcg atgagcatgc tcctgatcct gcccgcgctc
      781 ctgcacgcgg ccgggtaggc acctgcccgg cgaccaccac cgtcgtcgcc atacctaccg
      841 acccgaggag ctccgtcctg cccgcacgac gtccgacccg agttcttgcc gccctcgtca
      901 cgaccgccgc ccgggacgat ccgccaggtc ggctggtacc gctgcggacc ccggccgggc
      961 gagcagggca ccaccgtcct cgccgcccac gtcgacaccc gccgcgaggg tttggggccg
     1021 tgtgcccgac tccctgacct cacccccggg gcgagcgtca tcgtgaccgg ggcggaccgt
     1081 cataccgtgc ggttctcggt tcgttcggtc caccaggtcg ggaagtcgtc ggcgaccgtc
     1141 gaccggcttc tcgccgaccg atccagcgcg gggcagctcg cgctgatcac ctgcgggggt
     1201 cgttcacccg aggccgtggg tactccgaca acatcgtcgt cctggcgcag ctcgaccccg
     1261 cctcgtgacc cccagggcga gccaggcccg tgggcagtgc gaccatgagc catgactgat
     1321 gcaccctcgg gtctggtcct ggtcacgggc gcgaccggct acatcgggtc gcggctggtc
     1381 ccggccctgc tcgacgcagg ctgggcggtc cgggtcctga cccgctcggc ggccggggtc
     1441 gccgaccggg gctgggcgtc tcgggtcgag gtcgcgcagg gagacgccgc cgacccggcc
     1501 gtcctcgccg aggcgtgcgc gggggtccag gtcgcgtact acctgctgca ctcgatggac
     1561 ggacgtcccg acttcgaacg acgcgaccgc cgtatggcgc tcggcttcgc ccgcgcggcg
     1621 cgcgaggcgg gcgtcgggcg gatcgtctac ctcagcggcc tgcaccccga gggcgtgcgg
     1681 ctttcctcac acctggagtc gcggaccgag gtcgccgaga tcctgctcgc gagcggcgtg
     1741 ccgacgacgg tgctccaggc tgcggtgatc ctcggctcgg gctcggcgtc cttcgagatg
     1801 ctccggcacc tgacccggcg gctgcccgcc atggtcgccc cgaagtggct gaccaaccgg
     1861 atccagccca tcgccgtgcg cgacgtcctg acctatcttg tcgcctcggc gcagatgcct
     1921 tcacaggtca gccggacctt cgacatcggc ggccccgatg tgctgacgta caagcagatg
     1981 atcgggcgct tcgcgcagat cgacgggctg cgggcccgcc cgatcatgac cgtgccggtc
     2041 ctcaccccgt ggctggcgag ccactgggtc ggggtcgtca cgcccgtccc ggcctcgatc
     2101 gccaagcccc tcgtcgggag cctcatccac gaggtcgtgt gcaaggagca cgacatcgcc
     2161 ggcctcgtcc ccgacccgcc aggcggcctg ctcggcttcg acgaggcggt gcgggcggcg
     2221 ctggccggtg aggagtcgac ccggccggaa ccaggcaccg tcgaccccgc ctggctgacg
     2281 tcggccgacc cggactgggc cggcagctga gttctgacga aaccgttgtc ccctcgcaca
     2341 ggcgcgccta gttttggtcc cagttgccgc ctggcggtcg ccacctccca gcctgcggag
     2401 accagctcat gaaccctcgc tccgtccgtc cgctcattgc cgccgtcgcc gcactgggcg
     2461 cggtgctggc gggacccgcg ctggcggtgg cgccgagcca ggccgcgcaa gggccggcgc
     2521 tggggccggc gctgggctcg gcatcgggcc gggaagccgc ccccggtgac gtgcgcgtca
     2581 tccggtccct cacctcgctc accgcgacgc accggtggtt cgagcagacc tacggcgggc
     2641 tcccggtcgt cgacggcttc tacgtcgcgc gcacggacat ccgcacgggg gcccgctcgg
     2701 tcaccgacgg gcgccagggc
//
LOCUS       DATAIX010000353         2169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_18739, whole genome shotgun sequence.
ACCESSION   DATAIX010000353 DATAIX010000000
VERSION     DATAIX010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18739"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VHM65_05250"
     CDS             <1..249
                     /locus_tag="VHM65_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705146.1"
                     /translation="VGVLGVDAVPRPSGPVAAHDVLDADDQAVWGAMPARRGATIASL
                     AAAAGRSEGDVRAALGRLELFGFARRDGIGWKRLSGPS"
     gene            417..1307
                     /locus_tag="VHM65_05255"
                     /pseudo
     CDS             417..1307
                     /locus_tag="VHM65_05255"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
     gene            1537..>2169
                     /locus_tag="VHM65_05260"
     CDS             1537..>2169
                     /locus_tag="VHM65_05260"
                     /inference="COORDINATES: protein motif:HMM:NF022794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3152 domain-containing protein"
                     /protein_id="HEX2705147.1"
                     /translation="MCILLLGTVVARAAQSPTVALPDGVARVVTASTPVRQAIRAPSG
                     PTSATATPTTPGPSPEVTTPKPAVTASTSAAALVTKGAGTFTVVPIVGGANKATGRTV
                     YFTVEVEDGLPVDHGVFARTVRDILLDSRGWQTQDQRRFVPVSPARAKDGARVDIRVT
                     LASPTTTDRLCYPLQTGYAHVSCWSRGRSVINLYRWVKGASTFGTDLTSYR"
BASE COUNT          289 a          776 c          789 g          315 t
ORIGIN      
        1 gtcggagtgc tcggagtcga cgcggtgccg cgcccgagtg ggcccgtggc cgcccacgac
       61 gtcctcgatg ccgatgacca ggcggtgtgg ggtgcgatgc cggctcgccg gggcgcgacc
      121 atcgcgagcc tggcagcggc cgccggccgc agcgagggcg acgtccgggc ggctttgggg
      181 cgtcttgagc tcttcggctt cgcccgccgg gatggcatcg gatggaagcg gctcagcggc
      241 ccgtcctagc ccggtcagga ggtctgggca gccggtgcga cggcgccggt cgggccttcg
      301 tccggctgct gtcaccccag tgcggatgac gaccacgcac ggccgcaccg ggtctggttc
      361 ttgatcggga tgagggagtt tgcgacggtg gtgcggtgat caccgagacc aaggctgtcg
      421 tcgcggagtt cgcgcgccac ctcggtgccg agcgaagcct ctcggcccac accacccgcg
      481 cgtatgtcgg tgatgtcacg gcgctgctcg gctatgtcgg gcgcgagcgc ggccgggccg
      541 acctcgccga ggtcaccctg tccgacctgc gtgcctggct ggcggctcag tcccagcgtg
      601 gggcctcgcg agccacgctg gctcgacatg ccgcggccgc gcggaccttt ctgcggtggg
      661 cccatcgggt cggccggacc tcggccgacc cgtcgacccg tcttgtcgcg ccccgtcgag
      721 cgccgcatct gcccgcggtc ctgcgcaagg aggaggcggc cgcgctgctc gacgccgcca
      781 gggtgacggc cgatgacggc gacccgatcg gcgtgcggga cagagccatg ctcgagctgc
      841 tgtatgccac gggcatccgg gtcagtgagc tggtcggtct cgacctcgac gacctcgacc
      901 gggtgtccag gctcgtccgg gtcattggca aaggtaacaa ggagcgagcc gtcccgttcg
      961 gcatgcccgc ggacgatgct ctgaccgagt ggctcgccct ccggtcggag gtggttgtgg
     1021 ccgacagcgg tcccgcgatc ttcctcggcc ggcgcgggcg gcgggtcgac gcgcgccagg
     1081 tgcgtgccgc agtccaccag atgctgtcac acgtcccggg tgccccggac ctcggcccgc
     1141 acgggctccg ccacagcgcg gcgacgcact tgctcgacgg tggcgcggac ctgcggatgg
     1201 tgcaggagct gctcggccac gccagcctgg ccacgaccca gatctacacc catgtgtccg
     1261 ccgaccggct gcgccgatcc tacgagcagg cgcacccccg tgcctgatgg cccctcggac
     1321 tcccgcgcgt cccaagccct gcacggccag gcaacccgcg agaatggacc acatcgtggc
     1381 gcggccacgg tgatggagca cgagcgccga acgcggaagg acagcgtcat cggaacgata
     1441 gcgacgggga cgcgccagac gtcggtgggg cgctggggcc agccccaagg cctgcccgag
     1501 cgtcggcgcc gcctgcgccg gacggtgaca ctcacggtgt gcattctcct tctcggcacg
     1561 gtcgtggccc gggcggcgca gtcgccgacg gtggcgctgc ccgatggtgt cgcgcgcgtg
     1621 gtgacggcct cgaccccggt ccgtcaggcc atccgggctc cgtcggggcc gacgagcgcc
     1681 acggccaccc ccacgactcc agggccgtcg cccgaggtga ccacgcccaa acccgcggtg
     1741 actgcgagca cgtctgccgc tgcgctcgtg accaagggtg ccgggacgtt caccgtggtt
     1801 ccgatcgtcg gtggggccaa caaggcgacc gggcggaccg tgtacttcac ggtcgaggtc
     1861 gaggatggac tgcctgtcga ccacggcgtg ttcgcccgca cggtccgcga catcctgctc
     1921 gactcgcgcg ggtggcagac ccaggaccag aggcgcttcg tgccggtgtc cccggccagg
     1981 gccaaggacg gtgcccgggt ggatatccgg gtgactctgg ccagccccac cacgacggac
     2041 cgactgtgct acccgctgca gaccggctat gcgcacgtct cctgctggag caggggccga
     2101 tcggtgatca acctctaccg atgggtcaag ggtgcctcga ccttcggcac cgacctgacg
     2161 agctaccgc
//
LOCUS       DATAIX010000354         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_276473, whole genome shotgun sequence.
ACCESSION   DATAIX010000354 DATAIX010000000
VERSION     DATAIX010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276473"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VHM65_05265"
     CDS             <1..323
                     /locus_tag="VHM65_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705148.1"
                     /translation="VMFCAFEGRPRIVRLHGTGRVVLAGDAEFASLRREFTKVRTVGQ
                     RSVVVIDVERIADSCGFSVPLMEYVADRDVLDLTQLKRGATAYGDPSVVPSLDGLPGV
                     GRRV"
     gene            complement(385..732)
                     /locus_tag="VHM65_05270"
     CDS             complement(385..732)
                     /locus_tag="VHM65_05270"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2705149.1"
                     /translation="MTTTSTLLLEAALAKCCSRPVTVPLERAEADELAALLKAVADPV
                     RLQLVSILRSSPSMSACVCDLTPALGLSQPTVSHHLKVLTEAGVLTRERRGTWAWFTL
                     VPARLDQIARIVA"
     gene            975..1475
                     /locus_tag="VHM65_05275"
     CDS             975..1475
                     /locus_tag="VHM65_05275"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEX2705150.1"
                     /translation="MARVQLALNVVDLEASVTFYTAMFGVQPHKRRPGYANFVVADPP
                     LKLVLIQTTAEARGTGTAGALNHLGVEVEDTVAVDSARGRFQEAGMASFDELDTTCCY
                     ALQDKVWVHDPAGAPWEVYTVKDDNPADARPATASMPLLAAGEEPACCAPAAGSVCCA
                     PAGSAA"
     gene            1472..>2485
                     /gene="arsB"
                     /locus_tag="VHM65_05280"
     CDS             1472..>2485
                     /gene="arsB"
                     /locus_tag="VHM65_05280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEX2705151.1"
                     /translation="MSSPSYTSLSHDPVVAKLSTLDRFLPVWIGLAMVAGLVLGRLVP
                     GLNIALSSVEVDGVSLPIALGLLVMMYPVLAKVRYDRLDTVTADMRMLVSSLVLNWVI
                     GPALMFALAWLLLPDLPAYRTGLIIVGLARCIAMVIIWNDLACGDREAAAVLVALNSI
                     FQVVAFAALGWFYLQVLPGWLGLEQAALDVSAWQIATSVLIFLGIPLLLGYLSRRYGE
                     RARGRDWYESTFLARIGPWALYGLLFTIVILFALQGKAITSRPLDVARIALPLLAYFA
                     LMWGGGYLLGKALGMSYERTTTLAFTAAGNNFELAIAVAIATFGVTSGQALAGVVGPL
                     IEVP"
BASE COUNT          320 a          874 c          857 g          434 t
ORIGIN      
        1 tcgtgatgtt ctgcgcgttc gaggggcggc cgcgcattgt ccggctccac ggcaccggcc
       61 gggtcgtgct ggcgggcgat gccgagttcg cttcgctgcg aagggagttc accaaggtcc
      121 ggacggtcgg ccagcggtcg gtggtggtca tcgacgtcga gcggatcgcg gactcgtgcg
      181 gcttctcggt cccgctcatg gagtatgtcg ccgaccgtga cgtcctcgac ctgacccagc
      241 tcaagcgcgg ggcgacggcg tatggcgacc cttcggtggt cccgtcgctc gacgggctgc
      301 ctggggtggg ccgacgggtc tgacccccgc ccggcgccgg tctcgccctg aggcgaagag
      361 gtgcgctcag gcgaagaggt gcgctcaggc gacgatgcgg gcgatctggt cgaggcgcgc
      421 cgggacgagg gtgaaccagg cccaggtccc gcgccgctcc cgggtgagca ctccggcctc
      481 ggtgagcacc ttgaggtgat ggctcaccgt cggctgggac aagcccaggg cgggggtcag
      541 gtcgcagacg caggccgaca tcgacggcga ggagcgcagg atgctgacga gctgcagccg
      601 gaccgggtcg gccacggcct tgagcaaagc ggcgagctcg tcggcctcgg cgcgctccag
      661 gggcacggtg accggccggc tgcagcactt ggcgagcgcc gcctccaaca gcagcgtcga
      721 ggtagtcgtc atcgacgagc tccttcctgc tgctcgggct cgatccggtg tcgtgccgct
      781 tgacaggcgc gaatcgtgag atcggcccat gtctacgttg agccctgtct gtgttgagcc
      841 ctgtctgtgt tgacccgtgc ctatattggc acatgtctat attgacacat gtcgatgtcc
      901 cgtgtcacgc tggggcctgg atcgacgccg ggcgatacct tgaggctgaa cccgcccgaa
      961 ggagtgtcat cgtcatggcc agagtccagc tcgccctcaa cgtcgtcgac ctcgaggcgt
     1021 cggtcacgtt ctacaccgcc atgttcggtg tgcagccgca caagcggcgc ccgggctatg
     1081 ccaacttcgt cgtcgccgac ccaccgctca agctcgtcct catccagacc actgccgagg
     1141 ctcgcggcac cggcaccgcc ggcgccctca accatctggg ggtcgaggtc gaggacacgg
     1201 tggccgtgga ctcggcccgc gggcgcttcc aggaggccgg gatggccagc ttcgacgagc
     1261 tcgacacgac ctgctgctac gcgctgcagg acaaggtctg ggtccacgac cccgccgggg
     1321 cgccgtggga ggtctacacc gtcaaggacg acaaccccgc cgatgcccgc ccggcgaccg
     1381 cgtcgatgcc cctgctggcg gccggcgagg agccagcctg ctgtgccccg gcggccggct
     1441 ccgtgtgctg tgcccccgcc gggagcgctg cgtgagctca ccgtcataca cctccctctc
     1501 gcacgacccg gtcgtggcca agctgtcgac gctggaccgg ttcctgcccg tgtggatcgg
     1561 tctggccatg gtggccggtc tcgtcctcgg gcggctcgtc cccgggctca acatcgcgct
     1621 gagctcggtc gaggtcgacg gcgtctcgct gcccatcgcc ctcgggctgc tcgtcatgat
     1681 gtaccccgtg ctggccaagg tccgctacga ccggctcgac acggtgacgg ccgacatgcg
     1741 catgctcgtc tcctcgctcg tcctcaactg ggtgatcggc ccggcgctca tgttcgccct
     1801 ggcctggctc ctgctccccg acctcccggc ataccggacg gggctgatca tcgtcggtct
     1861 cgcgcggtgc atcgccatgg tcatcatctg gaacgacctc gcctgcggtg accgcgaggc
     1921 cgccgcggtc ctcgtcgccc tcaactcgat cttccaggtc gtcgcgttcg cggcgctcgg
     1981 gtggttctac ctgcaggtgc tgcccgggtg gctcgggctg gagcaggccg ccctcgacgt
     2041 gtccgcctgg cagatcgcca cgtcggtgct gatcttcctc ggcatcccgc tgctgctcgg
     2101 gtacctctcc cgccggtatg gcgagcgcgc ccggggccgc gactggtacg agtcgacgtt
     2161 cctggcgcgc atcgggccgt gggcgctcta cggactgctc ttcacgatcg tcatcctctt
     2221 cgccctgcag ggcaaggcga tcacctcacg gccgctcgac gtcgcccgga tcgcgctgcc
     2281 gctgctggcc tacttcgccc tgatgtgggg cggtgggtac ctgctcggca aggccctcgg
     2341 gatgagctac gagcggacga cgacactggc gttcaccgcg gccggcaaca acttcgagct
     2401 cgccatcgcg gtggcgatcg ccaccttcgg cgtgacgtcg gggcaggcgc tcgccggggt
     2461 cgtcggaccg ctcatcgagg tgccc
//
LOCUS       DATAIX010000355         2072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_18924, whole genome shotgun sequence.
ACCESSION   DATAIX010000355 DATAIX010000000
VERSION     DATAIX010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18924"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VHM65_05285"
     CDS             <1..381
                     /locus_tag="VHM65_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705152.1"
                     /translation="LLRHHLYRSERTGEPIHPELTRLHHPARWHYDILRCLDAFAEAR
                     VPYDPRMDDAIAILRARRCPDGRWAAARPYPGATHVPAPRAGTPSPWVTLIALRVLRC
                     YGGLLAADPRPSAQLRARSGTRGA"
     gene            complement(350..847)
                     /locus_tag="VHM65_05290"
     CDS             complement(350..847)
                     /locus_tag="VHM65_05290"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2705153.1"
                     /translation="MALTIRPLTVDDDLGSFGRIVLAAYLALPGHPREHGYDAELVDV
                     AGRVGQSTVLGAFDGVTPVGCVTYVADQASPHAENLLDGEASFRMLAVCSAGQRRGIG
                     EALVQRCVERARADRRAGVFIYSGDWMTAAHRLYGRLGFVRVTDRDWVLHDPPIRLLG
                     FRFAL"
     gene            949..1944
                     /locus_tag="VHM65_05295"
                     /pseudo
     CDS             949..1944
                     /locus_tag="VHM65_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          296 a          741 c          750 g          285 t
ORIGIN      
        1 ctcctgcgtc atcatctcta ccgcagcgag cgcaccggag agccgatcca ccccgagctc
       61 acccgtctgc atcacccggc tcggtggcac tacgacatcc tgcgctgcct cgatgccttc
      121 gccgaggcgc gagtcccgta cgacccgcgg atggacgacg cgatcgccat actgcgcgcc
      181 cgccggtgtc ccgacggtcg gtgggccgcc gcccgcccct atcccggggc aacccatgtc
      241 cccgctccgc gcgcgggcac gcccagcccg tgggtgaccc tgatcgcgct gcgggtgctg
      301 cgctgctacg gcggcctcct cgccgccgac ccgcgcccct ccgcgcagct cagagcgcga
      361 agcggaaccc gaggagcctg atcggcgggt cgtgcaggac ccagtcgcgg tcggtgacgc
      421 ggacgaagcc gagccgccca tagaggcgat gggcagcggt catccagtcg ccggagtaga
      481 tgaacacccc ggcgcgacgg tcggctcggg cccgctcgac gcagcgctgg acgagcgctt
      541 cgccgatgcc gcgccgttgg ccggctgagc acaccgcgag catccggaag ctggcctcgc
      601 catcgagcag gttctcggca tgagggctcg cctggtcggc gacgtaggtc acgcacccga
      661 ccggtgtgac gccgtcgaag gcgccgagca ccgtcgactg ccccacccga ccagcgacgt
      721 cgaccagctc ggcgtcgtag ccgtgctccc gcgggtgccc gggcaacgcg agataggcgg
      781 cgaggacgat cctcccgaac gacccgagat cgtcatcgac ggtgagcggc cggatggtaa
      841 gcgccacggt cgccacggta ccgctggccg gggcaggtca gcgcccaccg gagggcagtg
      901 ccgtgcctgg ccagttccgt cggcgggtgg cccgggagat gatggggtat ggcgaaccgt
      961 cccgcccccg cgctgacctt gcgtgaggag gaccgtgagg agctctcgcg tctggtccgg
     1021 gcctcgacgg ctcgtgcggg gtcggcgcag cgggccagga tcgtgctgtt gtccgcgcag
     1081 gggttctcga acacggcgat cgccgagctg gtgggggtgt ctcgtcccac ggtgatcggg
     1141 tggcgccggc gctaccaggc cttgggcctg gcggggctcg aggatgagct gcggtcgggg
     1201 cggccgcgca cgatcgacca tgaggcggtc gtgtccgcga cgctcgttcc gccgccgaag
     1261 gagctcgggg tgacgcactg gtcgacccgg ctgctggccc ggcacctggg gatcagtgac
     1321 gcgacggtgg ccaaggcgtg gcgcgagtcc cgggtcccgc cgtggcgggc cgagtcgttc
     1381 cggttctcca ccgaccccga gctcgtggcc aaggtcgtcg acgtggtggg gctgtacctg
     1441 aacccgccgg agaacgcggt cgtgctgtgc gtggacagga ggcacctccc ctttgcgggg
     1501 gaggagtccc ggatccaggc cgcggaccgg accggcacgg cgaccctgtt cgccgcgttg
     1561 gagatcgcca caggcagggt caccggcgcc tgcacgatgc accaccgccc cgaggagttc
     1621 ctggcgttcg tgcgccaggt gggcctggcc taccccgacg tggagttgca cctggtcatg
     1681 gacaacgacg ccacccacgg gcacccccac gtcgatgcct ggctcgcgga gaaccctcgg
     1741 gtgcggtgtc acttcacccc gaccaacgcg tcgtggatga acctggtcga ggtctggttc
     1801 tccctcatcg aacgccaagc catccgccgc ggcgtcctca cctccgtggg cgaggacctc
     1861 gaggccaggg tccgcgccgt catcgacggc tggaacgagc gcgcccaccc cttcgtgtgg
     1921 accaagaccg ccgaggagat cctcgccaga gccatccggg acaagaccgc gagcacaggg
     1981 agcaaggacg agccgccctg ggcgggccgg gcgcaacggc aaggagcctc cccccggtga
     2041 ctgggggagg ctcctcggtg atgagctggg cg
//
LOCUS       DATAIX010000356         2499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_19096, whole genome shotgun sequence.
ACCESSION   DATAIX010000356 DATAIX010000000
VERSION     DATAIX010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19096"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VHM65_05300"
     CDS             <1..750
                     /locus_tag="VHM65_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705154.1"
                     /translation="PRTQADEHAVALLASSPSLLAKVAAYVGTEDDDRRVDWSRIARV
                     ATEGAWSSTDRAFLGICAELGGYTSDGRQSEVTFGARWGALDGQHQAMIASVLTASAQ
                     TKKSEERDLLERLGVTFPDPLPQGSRAVRVRLGPDVPAVTGDALGRERVGFRDGLSPA
                     EIWLRGRGVWKMQAARVIDSQLLLIASSGIVRMAGTIDGITLHGERIAIIGRPLPQHP
                     LIGRPDPLDNASQNPVAYGDLDDLPGGPASG"
     gene            complement(805..>2499)
                     /gene="xdh"
                     /locus_tag="VHM65_05305"
     CDS             complement(805..>2499)
                     /gene="xdh"
                     /locus_tag="VHM65_05305"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HEX2705155.1"
                     /translation="QQALSPDAPLIHPRGNVLDIEHVKLGNPDHAIRNSAHVVTRKFK
                     TPWQEHAFMEPECAVAEPDGDGVRMYTSGQSVYDEQREIARMLALPRERVRSVSTLVG
                     GGFGGKEDMSVQHHAALMAWHLQKPVKVKFSRTESFNYHTKRHPMDMEFTVACDELGK
                     LTAVKVVILSDTGAYASLGGPVLQRACTHAGGPYSVPNFEVLGMALYTNNPVAGAFRG
                     FGVVQSQFAMESCVNELAEKVGLDAWEIRHLNVVRPGDALPNGQIADDSTGIAQVLDN
                     LKGDYYANPHAGLAVAYKNTGIGVGLKDTGRCILSVEDALVHIRTSAACMGQGVAQVV
                     MHVLCETVDLRSDQIVYELPDTARTPDSGTSTGSRQTLFSGEATRLAAVQLRDALEEA
                     GADLSRLEGRQWFGEFSPETDPIDSPKEHPVSHVGYGYGAQLVTLAPSGEVEKVLAVY
                     DVGTVMNQQSAVGQIEGGVMMGLGYGLTEEFVVEDGVPKTRYGQLGLIRSSKMPEVEV
                     RFVQGPGTLPYAYGAKGMGEICLIPTAPAAADAYYKRDGKRRYSLPLEDTFYKRPR"
BASE COUNT          354 a          888 c          866 g          391 t
ORIGIN      
        1 ccgaggaccc aggccgacga gcacgccgtc gcgctcctcg cgagctcacc gtccctgctc
       61 gccaaggtcg cggcgtatgt cgggaccgag gacgacgacc gccgggtcga ctggtcccgg
      121 atcgcgcggg tcgcgaccga gggagcctgg tcgagcacgg accgtgcctt cctcggcatc
      181 tgcgccgagc tcgggggata cacctcggac gggcggcagt ccgaggtcac cttcggcgcg
      241 cggtggggtg ccctcgacgg gcaacaccag gcgatgatcg ccagcgtgct caccgcgtcg
      301 gcgcagacga agaagtccga ggagcgggac ctgctggagc gcctcggcgt gacctttccc
      361 gacccgttgc cgcagggctc ccgggccgtg cgcgtgcgcc tcggccccga cgtcccggcg
      421 gtcaccggcg acgccctggg gcgcgaacgg gtgggtttcc gcgacgggct ctcgcccgcc
      481 gagatctggc tgcgtggccg gggtgtctgg aagatgcagg ccgcccgggt catcgactcg
      541 cagctgctcc tcatcgccag ctcgggcatc gtgcggatgg ccgggaccat cgacgggatc
      601 accctgcacg gtgagcgcat cgccatcatc ggccggccgc tgccgcagca cccgctcatc
      661 ggccggcccg acccgctcga caacgccagc cagaacccgg tcgcctacgg cgacctcgac
      721 gacctgcccg gcgggccggc ctccggctga ccacagcccc tgacaccccg ggcacggcgc
      781 gcgcgcgccg tgccggtcgc tcgctcaccg ggggcgcttg tagaaggtgt cctcgagcgg
      841 caggctgtag cgccgcttgc cgtcgcgctt gtagtaggcg tccgccgcgg ccggggcggt
      901 cgggatcagg cagatctctc ccatgccctt ggcgccgtag gcgtacggca gcgtgcccgg
      961 tccctggacg aaccggacct ccacctcggg catcttgctc gacctgatga ggccgagctg
     1021 gccgtaccgc gtcttgggca caccgtcttc gacgacgaac tcctcggtga gtccgtaccc
     1081 gaggcccatc atgacgccgc cctcgatctg acccaccgcg gactgctggt tcatgaccgt
     1141 cccgacgtca tacacagcca gcaccttctc gacctcgccg gacggggcga gggtgacgag
     1201 ctgagcgcca tagccatagc ccacgtggct gaccgggtgc tccttcggcg agtcgatcgg
     1261 gtcggtctcc ggggagaact cgccgaacca ctggcgaccc tccaaacggg acaggtccgc
     1321 gccggcctcc tcgagcgcgt cgcgaagctg cactgccgcc aggcgagtgg cttcaccgct
     1381 gaagagcgtc tggcgagacc cggtcgacgt cccgctgtcc ggcgtgcgtg cggtgtcggg
     1441 cagctcgtag acgatctggt cggagcgaag atccacggtc tcgcacagca cgtgcatgac
     1501 gacctgggcc acgccctgac ccatgcacgc cgccgacgtc ctgatgtgga ccaaggcgtc
     1561 ctcgacggac aggatgcacc ggccggtgtc cttgagaccg acaccgatgc cggtgttctt
     1621 gtaggcgacg gccaggccgg cgtgcgggtt cgcgtagtag tcgcccttga ggttgtccag
     1681 tacctgcgcg atgccggtgg agtcgtcggc gatctggccg ttcggcaggg cgtcgccggg
     1741 ccgcaccacg ttgaggtggc ggatctccca ggcgtcgaga cccaccttct cggccagctc
     1801 gttgacgcag ctctccatgg cgaactggct ctgcaccacg ccgaacccgc ggaacgcacc
     1861 ggccacgggg ttgttggtgt acaacgccat gccgagcacc tcgaagttcg gcaccgagta
     1921 gggaccgccg gcgtgggtgc aggcgcgctg caggaccgga ccgccgaggg aggcgtaggc
     1981 gcccgtgtcg gacaggatga cgaccttgac ggcggtgagc ttgcccagct cgtcgcacgc
     2041 caccgtgaac tccatgtcca tggggtggcg cttggtgtgg tagttgaagc tctcggtccg
     2101 ggagaacttg accttcaccg gcttctgcag gtgccaggcc atgagcgccg cgtggtgctg
     2161 gacgctcatg tcctccttgc ccccgaagcc gcccccgacg agggtggaca cggagcggac
     2221 ccgctctctc ggcagcgcca gcatccgggc gatctcgcgc tgctcgtcat acaccgactg
     2281 gcccgaggtg tacatgcgca caccgtcccc gtccggctcg gcgaccgcgc actcgggctc
     2341 catgaacgcg tgctcctgcc acggcgtctt gaacttgcgg gtcaccacgt gcgccgagtt
     2401 acggatggcg tggtcggggt tgcccagctt gacgtgctcg atgtcgagga cgttcccccg
     2461 cgggtggatc agcggtgcgt ccgggctcag ggcctgctg
//
LOCUS       DATAIX010000357         2035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_108883, whole genome shotgun sequence.
ACCESSION   DATAIX010000357 DATAIX010000000
VERSION     DATAIX010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108883"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VHM65_05310"
     CDS             complement(<1..784)
                     /locus_tag="VHM65_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2705156.1"
                     /translation="MSGQPLRVGVVGVGLMGADHAERVARRTANARLVAVSDPDEPLA
                     GALAAQYGVRAIADSLDLIADPEVDAVIIASPGFVHEEQVLACVEHGKYALCEKPLTM
                     DAESSLGVVRAERAAGRALVQVGFMRRFDPEYAQMQQMLASGAFGRLLLLHNTHRTKD
                     VPDTFRSEMIVRDSMVHEVDVARFLFGEEITEITVLSPAPATAAADGVVDPQVSIFRM
                     AGGAIVTNEVFVKNQVGYEVRCEAVADKGTIMAGRNWGGLYST"
     gene            complement(833..1732)
                     /locus_tag="VHM65_05315"
     CDS             complement(833..1732)
                     /locus_tag="VHM65_05315"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX2705157.1"
                     /translation="MAPRPVMIGSAPDSWGVWYADDPQQTPWQRFLDEVAAAGYTRIE
                     LGPYGYLPTDPAQLQDELERRGLVRTAGTICEHLHRKESWDSTWKEASAAARLSAAMG
                     AKHLVVIPGFWRDPTTGAVIEDAMLTDEEWPVYARQMNRLARAVREEFGLQIQFHPHA
                     DTHVDTHENVERFLDETDPELVLLCLDTGHISYCGGDNLTLIEDYPSRIGYVHLKAVD
                     PAIVEKVHAEGIGFGEAVRMGAMCEPPRGVPQMAPILRALAGLDVELFAIVEQDMYPC
                     PPDQPLPIATRTLAHLQSCGPLR"
     gene            complement(1735..>2035)
                     /locus_tag="VHM65_05320"
     CDS             complement(1735..>2035)
                     /locus_tag="VHM65_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705158.1"
                     /translation="VHSMAGLEAAVAEAKAAPAHGGPVVIHVETDPLVHAPGSMSWWD
                     VPVTPVSALESTQTAYETYARYKATQRGYLAPSQRATPAPSTAPSTSSASTTKGL"
BASE COUNT          292 a          699 c          732 g          312 t
ORIGIN      
        1 ccgtcgagta gagcccgccc cagttgcgtc cggccatgat cgtgcccttg tccgccaccg
       61 cctcgcagcg cacctcgtag ccgacctggt tcttgacgaa gacctcgttg gtgacgatcg
      121 cgccaccggc catccggaag atcgacacct gggggtcgac gaccccgtcc gcggccgccg
      181 tggccggcgc gggcgacagc acggtgatct cggtgatctc ctcgccgaac aggaaccggg
      241 cgacgtcgac ctcgtgcacc atgctgtcgc gcacgatcat ctccgaccgg aacgtgtcag
      301 gcacgtcctt ggtgcgatgg gtgttgtgca gcagcagcag ccggccgaac gcacccgacg
      361 cgagcatctg ctgcatctgc gcgtactccg ggtcgaaccg gcgcatgaag ccgacctgca
      421 ccagcgcccg tcccgccgcc cgctcggcac gcacgacccc cagcgaggac tcggcgtcca
      481 tcgtcagcgg cttctcgcac agggcgtact tgccgtgctc gacgcaggcg agcacctgct
      541 cctcatggac gaagccgggc gaggcgatga tgaccgcgtc gacctccgga tcggcaatga
      601 ggtcaaggga gtcggcaatc gcgcgcacgc cgtactgggc ggcgagtgcc ccggccagcg
      661 gctcgtcggg gtcggagacg gcgacgagcc tggcgttcgc ggtgcggcgc gcgacccgct
      721 cggcgtggtc ggctcccatg aggccgacac cgacgacacc gacgcgaagc ggctgtccag
      781 acatggctga ccattccttc tctctcgtat gacgtgcggg tggcggtcgc ggctagcgca
      841 gcggcccgca ggactgcagg tgggccaggg tgcgcgtggc gatcgggagc ggctggtccg
      901 gggggcaggg gtacatgtcc tgctcgacga tggcgaacag ctcgacgtcg agaccggcca
      961 gcgcccgcag gatcggggcc atctgcggca caccgcgggg gggctcgcac atcgcgccca
     1021 tcctgaccgc ctcgccgaag ccgatgccct cggcatggac cttctcgacg atcgccgggt
     1081 cgaccgcctt gaggtggacg tagccgatcc gcgacgggta gtcctcgatc agcgtgaggt
     1141 tgtcgccgcc gcagtaggag atgtggcccg tgtcgaggca gagcaggacg agctccgggt
     1201 cggtctcgtc caggaagcgc tcgacgttct cgtgggtgtc gacgtgggtg tcggcgtgcg
     1261 ggtggaactg gatctgcaga ccgaactcct cacgtaccgc gcgagcgagc cggttcatct
     1321 ggcgggcgta gaccggccac tcctcgtcgg tcagcatggc gtcctcgatg acggccccgg
     1381 tcgtcgggtc gcgccagaag ccggggatga ctacgaggtg cttggcgccc atggccgccg
     1441 agagccgcgc cgccgccgag gcctccttcc acgtgctgtc ccaggactcc ttgcggtgca
     1501 ggtgctcgca gatcgtcccg gcagtccgga cgagcccgcg ccgttcgagc tcgtcctgca
     1561 gctgcgccgg gtccgttggc aggtagccgt aggggccgag ctcgatccgg gtgtacccag
     1621 cggcggccac ctcgtcgagg aaccgctgcc acggagtctg ctgcgggtcg tccgcgtacc
     1681 acactcccca ggagtcggga gcgctgccaa tcatgaccgg gcggggcgcc atggctacag
     1741 acctttcgtc gtcgacgcac tggaggtgct gggcgcggtg ctgggcgcgg gggtggcccg
     1801 ttgcgacgga gcgaggtagc cccgctgcgt ggccttgtac cgcgcatacg tctcgtatgc
     1861 cgtctgcgtg gactcgagcg cggagaccgg ggtgaccggg acatcccacc acgacatcga
     1921 gccgggggcg tggacgagcg ggtcggtctc gacatggatg acgaccggtc cgccgtgcgc
     1981 gggcgccgcc ttcgcctcgg cgaccgcggc ctcgagcccg gccattgagt ggacc
//
LOCUS       DATAIX010000358         4756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_246769, whole genome shotgun sequence.
ACCESSION   DATAIX010000358 DATAIX010000000
VERSION     DATAIX010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246769"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..590
                     /locus_tag="VHM65_05325"
     CDS             207..590
                     /locus_tag="VHM65_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705159.1"
                     /translation="MDRSRLATLTVSGALGAAAAVVLYAAIAPSAATTPTSATPKTVA
                     VIPATPTVFAPCVEPAVLQGDTCVTNTTRIVTVPAPAPARQASTSRAMSVKTNAPVPA
                     STPDPSVREDHDDHHGGEDHEDHED"
     gene            complement(608..1900)
                     /locus_tag="VHM65_05330"
     CDS             complement(608..1900)
                     /locus_tag="VHM65_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2705160.1"
                     /translation="MRSQITRLVAATTSAVILAFVIPLCLLVRTLAEDRAGAATSQAA
                     QSVAILVSSVTDRDQLTAALTLVNEAGVSGTTVLLPTGEVLGAADPATALDPLVQRAQ
                     ATRAAFTERDGTGLTVLIPVVIAGGTAIVRSHVSSTDLHDGVMSAWLSILGLGAVLLA
                     TAVVVARELGHRISTPVSQVAAVAHRLRAGDFEARAEPGGTDEVVELATALNQLADRI
                     VELLLAEREVVADLSHRLRTPVTALRLDSELVPDGEVAARLRGHVDHLQRTVDAVVRE
                     ARRPVRTSMHGSCDAAAIVRDRVNFWRPLAEDQERLLHLVLPPGPILVPVAAEDMRDL
                     VDNLIDNVFAHTPEGCGFDVDLARDAGGVTRLTIADTGPGVPDGASATARGDSGSGST
                     GLGLDIVRRIARSAGGQVSLGRSAGGGLAVTVVFAPVG"
     gene            complement(1900..2589)
                     /locus_tag="VHM65_05335"
     CDS             complement(1900..2589)
                     /locus_tag="VHM65_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2705161.1"
                     /translation="MASILLVEDDLGIRTALVRSLTERGHAVTSSATGMDGLRRAIDD
                     APDLVVLDLGLPDIDGLQVLSMLRAVSQTPVIVATARDDDAVTVKALDAGADDYVVKP
                     YGAEQLEARVRAVLRRAGTTSPEGPITVGELEINAANRTVTLRGVEVELARKEFDLLH
                     LLAQRPGQVVTKREILAEVWRQVYGGGDRTVDVHLSWLRRKLGETAADPVYLHSVRGV
                     GVRLVDPGRQR"
     gene            2679..3170
                     /locus_tag="VHM65_05340"
     CDS             2679..3170
                     /locus_tag="VHM65_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705162.1"
                     /translation="MRRPNRTSTAVALWLLVVGASAAFAWFMINRAGREVMEGNQSIA
                     VLARAATGRPSSSSSPASPPATPTSAPTASTSSAGPGPTAESAPVTTTTRSVTLRGGT
                     VIAACAGSVVSLRSATPRNGWRLESPETEGDVLKVTFEAEGEDKSEVAVACVGGTPTF
                     STS"
     gene            3184..3816
                     /locus_tag="VHM65_05345"
     CDS             3184..3816
                     /locus_tag="VHM65_05345"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX2705163.1"
                     /translation="MAQPQSTRTLPTGPSSPVPLGSTDSTILLGEFRTSDMPALKACF
                     ADRETAVWNPGPADGDVAAWIALRNDWSDGAHLSWAVRGADGLLLGSLSIFKLDTVLS
                     SAQVGYFVFPGHRGYGVARRALALGVPFTFAERDIHRLELFHAVENGASCSVATAAGF
                     PLEGVLRQSYVYADGLRHDEHLHARLATDPIGGTDPVGARARGRPESTGR"
     gene            3931..4095
                     /locus_tag="VHM65_05350"
     CDS             3931..4095
                     /locus_tag="VHM65_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705164.1"
                     /translation="MKHRFFARTTGNCVTSAMPSSIVRGVRERPRITGSTSFGVRPQN
                     SSPRSMPTRT"
     gene            complement(4219..>4756)
                     /locus_tag="VHM65_05355"
     CDS             complement(4219..>4756)
                     /locus_tag="VHM65_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705165.1"
                     /translation="GYDIALVSTDPAATAAQIIERYAARWSIEVAIEDAKQTTGVGQA
                     RNRLATAVERTVPFELVITSLAICWYATTGYQPGDVRAARDLAPWYRDKTQPSVADML
                     TMTPPGDHRRTITASRPPAGHSQRNQHPSAGLGEHRRITAKVEGYGAPRASRGGRPRP
                     RAPVVVKEHAPSARRWVP"
BASE COUNT          697 a         1736 c         1647 g          676 t
ORIGIN      
        1 caacccctcc atccgagatc cgagatccca gatcccagat cccagatccc agatcccaga
       61 tcccagatcc cagatcccag acgagctctt agcgcggagt tgacggtccg tgaggtcggc
      121 cttgaggtcc gctggcccac gatcacagga ccggaggcgc accggcctcg ggacaagccg
      181 gacaaccaca cacaaggaga ctcccgatgg accgctctcg tttggccacc ctcacggtca
      241 gtggcgctct cggcgcggcc gccgcggtcg tgctctacgc agccatcgcc cccagcgcgg
      301 cgacgacacc gacgtcggcc accccaaaga ccgtggccgt gatccccgcg acgccaaccg
      361 tgtttgcccc ctgcgtcgaa cccgcagtcc tgcaaggcga cacctgcgtc accaacacca
      421 cccgcatcgt cacggtgcct gccccggcgc ccgcacgcca agcgagcacg agccgcgcca
      481 tgtcggtcaa gaccaacgcg cccgtcccgg catcgacgcc cgacccgagc gtccgtgaag
      541 accacgacga ccaccacgga ggcgaggacc acgaggacca cgaggactga gcgctggctc
      601 ggggccgtca gccaacgggt gcaaagacga ccgtgaccgc cagccccccg ccggccgacc
      661 ggcccaggct cacctgtccc cctgcgctgc gggcgatcct gcgcacgatg tcgagcccca
      721 gcccggtgga gccgctgccg ctgtccccgc gcgcggtcgc ggacgcgccg tcgggcactc
      781 ctgggccggt gtcagcgatc gtgagacggg tcacgccgcc agcgtcacgc gcgagatcga
      841 cgtcgaagcc acacccctca ggagtgtggg cgaacacgtt gtcgatgagg ttgtcgacca
      901 ggtcgcgcat gtcctcggca gccacaggca cgagaatcgg gcccgggggc agcacgagat
      961 gcaggaggcg ctcctggtcc tcggcgagcg gccgccagaa gttcacccgg tcacgcacga
     1021 tcgcggccgc atcgcaggac ccgtgcatcg aggtccggac cggtcggcgt gcctcgcgga
     1081 cgacggcgtc gacggtccgc tggaggtggt cgacatgacc ccgcagacgt gcggcaacct
     1141 cgccgtccgg gacgagctcg gagtcgagtc gcagggcggt caccggcgtc cgcagccggt
     1201 gcgacaggtc ggcaaccacc tcccgctcgg ccagcaacag ctcgacgatc cggtcagcca
     1261 gctggttcag cgcggtcgcc agctcgacca cctcgtccgt tccgcccggt tcggcccggg
     1321 cctcgaagtc cccggcgcgc agccggtgcg cgacggcagc cacctggctc actggagtgc
     1381 tgatccggtg cccgagctcg cgggcgacga cgacggccgt ggcgagcagc acggcgccca
     1441 ggcccaggat gctcagccag gcggacatca ccccgtcgtg caggtcggtc gagctgacgt
     1501 gcgagcggac gatcgcggtg ccccccgcga tgacgaccgg gatgaggacc gtcaggcccg
     1561 tgccatcgcg ctccgtgaat gcggccctgg tcgcctgggc ccgctggacg agagggtcga
     1621 gagcagtcgc cggatcggcc gccccgagca cctcccccgt cggcagcagc accgtcgtgc
     1681 cggagacgcc tgcctcgttg accagcgtca gcgcggcggt cagctggtcc cggtcggtga
     1741 ccgacgacac gaggatcgcg acactctgtg cggcctgcga cgtcgcggcg ccggcccggt
     1801 cctcggcgag ggtccggacg agcaggcaca gcggtatgac gaaggccagg atgacggccg
     1861 aggtcgtggc ggcaacgagc cgggtgatct ggctgcgcat cagcgctgtc gaccagggtc
     1921 gacaagccgg actccaaccc cccgaacgct atgcaggtag accggatccg cggcggtctc
     1981 gccgagcttg cggcgcagcc aggacaggtg cacgtcgacg gtgcgatcgc cgccgccgta
     2041 gacctgtcgc cacacctcgg ccaggatctc gcgcttcgtc acgacctgcc ctggtcgctg
     2101 cgcaagcagg tggagcaggt cgaactcctt gcgggcgagc tcgacctcga ccccacgcag
     2161 ggttaccgtc cggttggctg cgttgatctc gagctcaccg accgtgatcg gcccctcggg
     2221 gctggtcgtc ccggcccggc gcaggacggc ccgcacccgc gcttcgagct gttcggcgcc
     2281 atacggcttg acgacgtagt cgtccgctcc agcgtcgaga gccttgaccg tcaccgcgtc
     2341 gtcgtcgcgc gcggtggcca cgatgaccgg cgtctggctc accgcccgca gcatcgacag
     2401 cacctgcagc ccgtcgatat cgggcaaccc gagatcgagc accacgaggt ccggagcgtc
     2461 gtcgatcgcg cggcgcagcc cgtccattcc cgtggcgctg ctggtcaccg cgtgcccgcg
     2521 ctcggtgaga gaccgcacca gtgccgtgcg gatgccgagg tcgtcctcga cgagcaggat
     2581 ggaagccacg gttgttaccg tacgctcaag ccgagccggg tgggccgtcg ccgccgcggg
     2641 ctcacgccga cgcgagtcgg cgcgacgagg agcctgccat gagacgaccg aaccgaacca
     2701 gcaccgcagt cgccctctgg ctcctcgtcg tgggggcctc ggccgccttc gcctggttca
     2761 tgatcaaccg ggccggccgc gaggtgatgg agggcaacca gtcgatcgcc gtcctcgctc
     2821 gtgccgccac cggccgacct tcctcgtcct cgtcgccggc cagcccgccc gctacgccga
     2881 catcggcacc gacggcgtcc acctccagcg caggcccggg gcccacggcc gagtcggctc
     2941 cggtcacgac gaccacgcgc tcggtcacgc tgcgcggagg cacggtgatc gccgcctgcg
     3001 ccggctccgt ggtttcgctg cgctcggcga cgccgcgcaa cgggtggcgt ctggagtccc
     3061 ccgagaccga aggggatgtc ctcaaggtca ccttcgaggc cgaaggggag gacaagtcgg
     3121 aggtcgccgt tgcctgcgtc ggtggaaccc ccacgttctc cacgtcgtga cacactcccc
     3181 ggtatggcgc agccgcagtc cacccggacc ctcccgacgg ggccgagcag ccccgtcccg
     3241 ctcggctcca cggacagcac gatcctgctc ggcgagttcc ggaccagcga catgccggcg
     3301 ctcaaggcct gcttcgccga tcgcgagacc gccgtgtgga acccgggccc ggccgatggc
     3361 gacgtggcgg cgtggattgc cctgcgcaac gactggtccg atggcgcaca cctctcctgg
     3421 gcggtccgcg gtgcggacgg cctcctgctc gggagcctgt cgatcttcaa gctcgacact
     3481 gtgctgtcgt cggcgcaggt cggctacttc gtcttccccg ggcaccgcgg gtatggcgtg
     3541 gcgcggcgag cgctcgcgct gggcgttccc ttcaccttcg cggagaggga tattcaccgg
     3601 ctcgagctgt tccacgcggt cgagaacggg gcgtcgtgct cggtcgcgac cgcggccggc
     3661 ttccccctcg agggtgtcct gcgccagtcc tacgtgtatg ccgacggcct gcgccacgac
     3721 gagcatctgc acgcgcggct cgccacggac cccatcggcg gcacggaccc tgtcggcgcc
     3781 agggcgcgcg gccgccccga gtcgacgggc cgctgaggcg actccgagcc acgaatgcgg
     3841 gttgccgtcc cagcaccagc cgagcttcgg cgcccgctcg ctgatccaga gtgcgggacc
     3901 cgccggtcgt ggcgccgtga ccccgacagc atgaagcacc ggttcttcgc caggaccacc
     3961 gggaactgcg tgacgagcgc gatgccctcc tcgatcgtca ggggggtccg cgagcggccc
     4021 aggatcaccg gcagcacctc cttcggtgtg cgcccgcaga actcctcgcc gcgctcgatg
     4081 ccgacgagga cgtaggccgg cgcgtcggga acgtcgagct cggggatcgg ctggtaggtc
     4141 gcgaggtcac caggggcgtg gttgcggtcg accacgtcca cctcggcgag accggcgatc
     4201 gtcggggagc gcagggcctc acggcaccca acgccgtgcc gacggtgcgt gttccttgac
     4261 gaccacaggg gccctcggcc gtgggcgacc tccgcgcgag gctcgcggcg cgccataccc
     4321 ctcgactttc gcagttatgc ggcggtgttc tcccaggcca gccgaaggat gttgatttcg
     4381 ttgggagtgg ccggctgggg gtctgctggc cgtaattgtg cggcgatgat cacccggcgg
     4441 agtcatggtg agcatgtcgg cgaccgaggg ttgggtcttg tcgcggtacc agggggcgag
     4501 gtcgcgtgct gccctgacgt cgccgggctg gtagccggtg gtggcgtacc agcagatcgc
     4561 caggctggtg atgaccagct cgaacgggac ggtgcgctct accgcggtgg ccagccggtt
     4621 gcgggcctgg ccgacgccgg tggtctgttt cgcgtcttcg atggcgactt cgatgctcca
     4681 gcgggcggcg tagcgttcga tgatctgcgc ggcggtcgcg gccgggtcgg tgctgaccag
     4741 ggcgatgtcg tagccg
//
LOCUS       DATAIX010000359         4274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_168821, whole genome shotgun sequence.
ACCESSION   DATAIX010000359 DATAIX010000000
VERSION     DATAIX010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168821"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VHM65_05360"
     CDS             complement(<1..818)
                     /locus_tag="VHM65_05360"
                     /inference="COORDINATES: protein motif:HMM:NF019542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HEX2705166.1"
                     /translation="MPGDVIPLFGRDPEPRAQWEVPPPPAEPARYLLRIELMESEPVV
                     WRSVEVASDLTMDVLHEVIQAAMGWQDCHLHAFVLGSADDYGAPRLLTEADTDEGEDG
                     TQEVDVRLDQVLRQPDERIGYEYDFGDSWSHEITLEDIRPHDPTAPRAVCLDGARACP
                     PEDIGGIHSHNVVVAWLSGQPGVEPDEPELTRQWLPEDYAPAQFSVTEATERVALTRA
                     GAALSHLDLREPVLDMLGRLGSARPLLVAMVHDAFGEPTRAASTPDPPAGLTDG"
     gene            1706..3016
                     /locus_tag="VHM65_05365"
     CDS             1706..3016
                     /locus_tag="VHM65_05365"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX2705167.1"
                     /translation="MTSTVLSGRLPTAVARLSPRWLPSLRTALVVTACLLSSTLAVRT
                     GQARHSIAAGLPVTESLTGRSLLLWFVGLGVAVAMVWRHRWPVGLALGTAALTLVLPL
                     DSLAALVATTIVVIRHRGREVWVVVAATSAATFASVWRDSRGTSSADSFWRLVLAEPG
                     MGGAGEPFGWWLPVVIAAAVVAGTVGIALLRRSRADFQLARDAEVAARDEASALSAQV
                     ARQAERERIAREVHDALGHRLSLLSLHAGALEVAAAGDDPRVRASAALVRQSAQQSMA
                     DLRSLLTVLRTPADSNIAEAVPDLRDVQALVDENLRVGIPTVSTVSFDSVADLDPATG
                     RAAYRVLQELLTNARRHAPGIPVRALVTAAPQHGVDIEVANHVSQDAGTVVALGSGLT
                     GVAERAGQLGGEARFWVDEGRVFRAAILLPWVWAAADDGGRRRG"
     gene            3009..3707
                     /locus_tag="VHM65_05370"
     CDS             3009..3707
                     /locus_tag="VHM65_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2705168.1"
                     /translation="MGEATGAGSDLARPIRVLLVDDDPLVCSGLSLMLSVAPDLAVVG
                     AVHDGDEVIGAIHRHRPDVVLMDLRMRRQGGIKTTESLTGMPSRPKVLVLTTYDLDEV
                     LLGALRAGADGFLLKTASPTEIMAGIRSVHAGQGVLSPMSAGQAIRSVHGDPGVEARR
                     AAAATSMAGLTGRERAVCVLVARGMTNSAVGARLFMSEATVKTHLASAQARLGVGNRV
                     EVAVLIERVGLLDE"
     gene            complement(3747..>4274)
                     /locus_tag="VHM65_05375"
     CDS             complement(3747..>4274)
                     /locus_tag="VHM65_05375"
                     /inference="COORDINATES: protein motif:HMM:NF021599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 family protein"
                     /protein_id="HEX2705169.1"
                     /translation="VPVPARLPPRASGIGLDLAPIDVHDDEAVRWLEACVWPDQGERF
                     QRLVAAVALARATPPDVRRGDAVEHVAALVREAARSGHPVLTTTWVLNYLGPAGQAGF
                     VAELDRVGAELDLSWVIAESPAQSPALPVPTTATPEELTVLSLVRWRDGARTVERLGT
                     THPHGFWLHWEPALA"
BASE COUNT          539 a         1432 c         1647 g          656 t
ORIGIN      
        1 tccccgtcgg tgagcccggc gggcgggtct ggggtcgatg cggctcgtgt gggctcgccg
       61 aaggcgtcgt gcaccatggc gacgagcagg ggacgggccg acccgagccg tccgagcatg
      121 tcgaggacgg gctcgcgcag atccaggtgg gagagcgcag caccggccct ggtgagggcg
      181 acccgctcgg ttgcttcggt gacgctgaac tgtgccgggg cgtagtcctc gggtagccac
      241 tgacgcgtca gctcgggctc gtcgggttcg acgccgggct gcccgctgag ccaggcgacg
      301 acgacgttgt ggctgtggat ccccccgatg tcctcgggcg ggcacgccct tgcgccgtcg
      361 aggcacacgg cccgcggagc ggtggggtcg tgcgggcgga tgtcctcgag ggtgatctcg
      421 tgcgaccagc tgtcgccgaa gtcgtactcg tagccgatcc gctcgtccgg ctgacgcagg
      481 acctggtcga ggcgcacgtc gacctcctgc gtgccgtcct ccccctcgtc ggtatcggcc
      541 tcggtgagca ggcgcggggc cccgtagtcg tcggccgatc cgaggacgaa ggcgtgcagg
      601 tgacagtcct gccaccccat cgcggcctgg atgacctcgt gcagcacgtc catggtgagg
      661 tcgctcgcga cctcgacgga ccgccatacc acgggctccg actccatcag ctcgatgcgc
      721 agcaggtagc gcgcgggctc agcgggaggt ggggggacct cccactgagc ccgcggttcg
      781 gggtcacggc caaacagggg tatgacgtcg ccgggcactg ggtctcctct cgcctggccc
      841 gaccctatgc gactgaggcc gcagggcgct cgacgcggtc ccgcgccgcc ggtgctggcc
      901 ggccggatcg gcctcggtgt tgggcggcgc gggtagtgag gtggatctgg ccgggtgact
      961 cggcgtcacg tcgactggcg ggggatgtat ccccgacggc cggtgcccct cctactgggt
     1021 atcgaccatc gcagagagga gcacgacatg cgagcagcag ccatctgggt gcggattgtg
     1081 cagcgccagt ccgtctaccg accgcccgca ggtcggcgtc catctgcgct gaccacacga
     1141 cgacccgggg tccggacaag ccccgccgcg ccggcgatca gcccgcaggc gccgcggatg
     1201 ttgcatccgg actcaccggg ctacgcctga cacgacgcag ggccgccgac cagcgtggtc
     1261 ggcggccctg cgttctgccg tcaggttgtc aggagtgagg cgggcgtcat accccccggt
     1321 atgacgccca cctcgcacct gggtgggcta cagcgtgacg gtcaccccgc cggacagcat
     1381 cgagtcgtgc ggctcgatcg gggccacttt ggcgcccttg ggcatgtcgt agacgaggat
     1441 gccctcaagc gagttgccgg ggctgatctc aatgaatatg accgagtttg ccttaagtaa
     1501 gcatcccggc cgtcgagctt tccttgcatg gtcccgcggt ggatccgggc tgggtccttc
     1561 gggtgccgcc agtccatcac ctgcacggct gaggcgtctt cagccgttcg gatgggagca
     1621 gatcatccca tcggcttggg gtcgatgtgt ctagagtcgg ctgagggaga ctcggctgcg
     1681 catggacgcg gatatggtga gtgacatgac gagcacggtc ctttctggac ggctgccgac
     1741 cgcggtggct cgtctgtctc ccaggtggct gccttcgctg cggaccgctc tggtcgtgac
     1801 ggcgtgtctg ctgagcagca cgctggcggt caggactggc caggcgcgcc acagcatcgc
     1861 tgccggcctc ccggtgaccg agtcgctcac cgggaggagc ctgctcctgt ggttcgtggg
     1921 gctcggggtc gctgtcgcga tggtatggcg gcatcggtgg cccgtcggcc tcgctctcgg
     1981 caccgccgcg ctgacgctcg tgctcccgct cgactccctt gccgcccttg tcgcgacgac
     2041 catcgtggtg atccgccacc gaggacgcga ggtgtgggtg gtggtggccg cgacgtcggc
     2101 ggcgaccttt gcgtcggtgt ggcgcgactc gcgtggaacg agctcggcgg actcgttctg
     2161 gcggctggtc ctggccgagc cggggatggg tggggccggc gagccgttcg ggtggtggct
     2221 ccccgtcgtc atcgcggcgg ccgtcgtcgc cgggacggtc gggatcgccc tgctgcgacg
     2281 aagccgggcc gacttccagc tggcgcgcga tgccgaggtt gctgcgcggg atgaggcttc
     2341 ggcactctcc gcccaggtgg cgcgccaggc cgagcgggag cggatcgccc gcgaggtgca
     2401 cgacgcgctg gggcaccgcc tgtcgctcct gtccttgcac gccggggcgc tcgaggtggc
     2461 cgcggccggc gacgacccgc gggtgagggc gtcggccgcg ctggtccgcc agagcgccca
     2521 gcagtccatg gccgacctgc gctcgttgct caccgtgctg cgcacccccg cggactcgaa
     2581 catcgccgag gcggtgcccg acctgcgcga cgtgcaggcg cttgtcgacg agaacctgcg
     2641 cgtcggcata ccgacggtgt cgacggtgtc gttcgactcg gtcgccgacc tcgaccccgc
     2701 gaccggccga gcggcctacc gggtcctgca ggagctgctg acgaacgccc ggcgtcacgc
     2761 gccgggcatc ccggtccgcg cgctcgtgac cgcggcgccc cagcatgggg tcgacatcga
     2821 ggtcgcgaac cacgtgtcgc aggacgcggg cactgtcgtc gcgctgggca gcgggctcac
     2881 cggcgtggcc gagcgggcgg ggcagctcgg gggcgaggcg aggttctggg tcgacgaggg
     2941 gcgggtgttc cgggcggcca ttctcctgcc ctgggtgtgg gcggctgcgg acgatggcgg
     3001 caggcgccgt gggtgaggcc accggggcgg gctccgacct ggccaggccg atccgcgttc
     3061 tgctcgtcga cgacgacccg ctcgtgtgca gcgggttgtc gctgatgctg tcggtggcgc
     3121 cggaccttgc cgtggtcggg gcggtgcacg acggcgacga ggtcatcggg gcgatccatc
     3181 ggcaccggcc ggacgtcgtc ctgatggatc tgcgcatgcg tcgtcagggt ggcatcaaga
     3241 caaccgagtc cctgaccggt atgccgtcgc gaccgaaggt cctcgtgctg acgacgtacg
     3301 acctcgacga ggtactactg ggggcgttgc gcgcgggggc ggacggattc ctgctcaaga
     3361 cggcctcgcc gacggagatc atggcgggga tccgcagcgt ccacgccggt cagggagtgc
     3421 tgtcgccgat gagcgcaggg caggcgatca ggtccgtgca cggcgacccc ggggtggagg
     3481 cgcggcgtgc ggcggctgcc acgtcgatgg ccgggctgac cgggcgggag cgcgcggtct
     3541 gcgtgctcgt ggcccggggg atgaccaact cggccgtcgg ggcgcggctg ttcatgagcg
     3601 aggcgacggt caagacgcac ctcgccagcg cgcaggcacg gctgggtgtg ggcaaccggg
     3661 tcgaggtggc ggtgctgatc gagcgggtcg gcctgctgga cgagtgaccg gggggtcgtg
     3721 accggctcgt cggcgcgcgt gccgggtcag gccaaggccg gctcccagtg cagccagaac
     3781 ccgtgcgggt gggtcgtgcc cagacgctcg accgtccgcg cgccgtcgcg ccagcggacc
     3841 agcgagagca ccgtcagctc ctcgggcgtg gcagtggtgg gcaccggtag cgcgggggac
     3901 tgagccgggc tctccgcgat gacccacgac aggtcgagct cggccccgac ccggtccagc
     3961 tcggcgacga accccgcttg cccagccggt ccgaggtagt tgagcaccca ggtcgtcgtg
     4021 agcacgggat ggccggagcg ggcagcctcg cgcaccagcg ccgccacgtg ctcgaccgcg
     4081 tcgccgcggc gcacgtccgg cggggtcgcc cgggcgagag cgaccgcggc cacgagccgc
     4141 tggaagcgct ccccctggtc gggccagacg catgcctcga gccagcggac cgcctcgtcg
     4201 tcgtgcacgt cgatgggagc gaggtcgagc ccgatcccgc tcgcccgtgg ggggagccgg
     4261 gcagggacgg ggac
//
LOCUS       DATAIX010000360         3169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_127624, whole genome shotgun sequence.
ACCESSION   DATAIX010000360 DATAIX010000000
VERSION     DATAIX010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127624"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="VHM65_05380"
     CDS             <1..1171
                     /locus_tag="VHM65_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2705170.1"
                     /translation="AVLLTAALATAGCAESKTSTDAPASGSSAASGPVTIGAVLDITG
                     AGASLGVPERQTLEMLVEKANAAGGVAGRKISLIIEDDQSTEEGAAKAATKLLTSDKV
                     DILLGASRTGPSLAMRPLAEQAQKPMISLAANIKIVQGAKWVFKTAQNDRVVLERLLD
                     DMKSKGYTKLAIARDASGYGEGVSETVTELGKSQGLQVVAVEKFAPDATDFTAQMVKI
                     RNAKPDSVLIWGIAPAAALAQVAYRQLNLTAPLYQSHGIGNKAFLDAAGPAANGVLVP
                     LGRIAVADQLPDSDPQKAVLTSFIADFTAKYSQAPSGFAGYAYDAFTIAVDAVKKVGT
                     DANKLRDAIEGLTNYAGISGVFSMTPDNHSGLSKDAVVMVQVTDGAWKVAKGTSG"
     gene            1168..2037
                     /locus_tag="VHM65_05385"
     CDS             1168..2037
                     /locus_tag="VHM65_05385"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX2705171.1"
                     /translation="MSDLLQLVFSGLAHGAVYALVALGFVAVFAVREIVNLVQGEYAA
                     LAGLSAISAVAAGVPLALACLVVVPLVVLVAVLLERVAIAPLKSITPLVSIILTLGLS
                     TAVKAVMLLIWGPQAQRLPVFAGTDLLIGGVSIRAQELWIIGVAVLVGFAVVWLYEHT
                     LAGKALRACAEQPVAARLVGISPRTATMAAFAVAGFVGAVAGVIGTPIYLTSWDHGLA
                     LGLKGFVAATLGGLLSFRVAMAGGLILGVLENLVAGYVNTGYRDAVAFVVLLLVLVLR
                     PQGLALKASGARV"
     gene            2034..>3169
                     /locus_tag="VHM65_05390"
     CDS             2034..>3169
                     /locus_tag="VHM65_05390"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX2705172.1"
                     /translation="MIERLRRLVPFGILTLALVVFPVVAPTNTVNVGVFALIYGLAAI
                     GLSLLMGLAGQVSLGHAAFFAVGAYTQAMLVTKTGTPWFAAAGFAVLAAMLVALLVGL
                     PLLRLRGHFLALATLGLGIILSVILRELEVTGGTSGIFGIPKPELGGRVYDSPQEFFW
                     FMTPIVVLGLMMAANLVRSRTGRALGAVNDSEVAAECLGVDTFALRLRVFVLAGGYAG
                     LAGACYAHWVSVVSPEVSGFGLSVRMLLIVVLGGLGSVWGAVTGAVAVEILDEGLRTV
                     IPKLIPGAAGEVQLLGFGVILVLLVVLLPGGLDQLWQALTAGIRRVRNQVASAAALPP
                     VDDQGHDVALATLLGEAQAPERGSLVLRVRGVAKHYGGVKALADL"
BASE COUNT          430 a         1126 c         1086 g          527 t
ORIGIN      
        1 cgcggtgctg ctcaccgccg cgctggctac cgcggggtgc gcggagtcga agacctcgac
       61 cgatgcgccc gcgtcgggct cgtccgcggc gtcaggcccg gtcacgatcg gcgcggtgct
      121 cgacatcacc ggagccggcg cgagcctggg cgtccccgag cgccagaccc tggagatgct
      181 cgtcgagaag gccaacgcgg ccggtggggt tgccggacgc aagatcagcc tcatcatcga
      241 ggacgaccag tccaccgagg agggggccgc caaggcggcg accaagctcc tcacgtcgga
      301 caaggtcgac atcctgctcg gagcatcgag aaccggaccc agcctcgcca tgcggccact
      361 ggccgagcag gcacagaagc cgatgatctc cctcgccgcc aacatcaaga tcgtccaggg
      421 cgccaaatgg gtcttcaaga ccgcgcagaa cgaccgcgtc gtcctcgagc ggctgctcga
      481 cgacatgaag tccaaggggt acaccaagct cgcgatcgcc cgagatgcct ccggctacgg
      541 ggagggcgtg tccgagaccg tcaccgagct gggcaagtca cagggcctgc aggtcgtcgc
      601 cgtcgagaag ttcgcgccgg acgcgacgga cttcaccgca cagatggtga agatccgcaa
      661 cgccaagccg gactcggtgc tcatctgggg gatcgcgccg gccgcagccc tggcgcaggt
      721 cgcctaccgc cagctcaacc tcaccgcccc cctctaccag tcccacggga tcggcaacaa
      781 ggccttcctc gacgcggccg ggcccgcagc caacggcgtg ctcgtgccgc tcgggaggat
      841 cgccgtggcc gaccagctgc cggacagcga cccgcagaag gccgtgctca caagcttcat
      901 cgccgacttc acggcgaagt acagtcaggc gccctccggc ttcgccgggt atgcctacga
      961 cgccttcacg atcgccgtcg acgcggtcaa gaaggtcggc accgacgcga acaagcttcg
     1021 ggacgccatc gagggactga cgaactacgc gggaatcagc ggcgtgttct cgatgacccc
     1081 ggacaaccac tcgggcctga gcaaggacgc ggtcgtcatg gtgcaggtca ccgacggagc
     1141 gtggaaggtc gccaagggca cctcgggatg agcgatctcc tccagctggt cttctcggga
     1201 ctggcccatg gagcggtcta cgccctggtg gcactcgggt tcgtcgcggt cttcgccgtt
     1261 cgcgagatcg tcaacctcgt ccaaggtgag tacgccgcgc tcgccggact gagcgccatc
     1321 agcgccgtcg cggcaggtgt cccgctcgcg ctcgcctgcc tcgtcgtcgt gccgctcgtc
     1381 gtcctcgtcg cggtcctgct cgaacgcgtc gccatcgccc cgctcaagtc gatcaccccg
     1441 ctcgtgtcga tcatcctcac gctcggtctg tccaccgcgg tcaaggccgt catgctgctc
     1501 atctggggcc cgcaggccca gcggctgcca gtgttcgccg ggacggacct gctcatcggc
     1561 ggggtgagca tccgggcgca ggagctatgg atcatcgggg tggcggtact cgtcggcttc
     1621 gcggtcgtct ggctttacga gcacacgctg gccggcaagg ccctgcgggc gtgcgcggag
     1681 cagccggtgg ccgcgcggct cgtcgggatc tcgccgcgga cggcgacgat ggcggccttt
     1741 gccgtggccg gcttcgtcgg ggccgtggct ggggtcatcg gcacgccgat ctacctcacc
     1801 tcgtgggacc acgggctggc cctcgggctc aagggcttcg tggcggccac cctcggcggg
     1861 ctgctctcgt tccgggtggc catggccgga ggactgatcc tcggggtcct cgagaacctc
     1921 gtcgcgggct acgtcaacac ggggtaccgg gacgccgtgg ccttcgtcgt cctcctcctc
     1981 gtgctcgtcc tgcggcccca gggccttgcc ctcaaggcgt cgggagcacg ggtgtgatcg
     2041 agcgcctccg ccggctggtc cccttcggga tcctcaccct tgccctcgtc gtgttccccg
     2101 tcgtcgctcc gacgaacacg gtcaacgtcg gggtcttcgc gctcatctac gggctggcgg
     2161 ccatcggcct gtcgctgctc atggggctcg ctgggcaggt cagcctcggc cacgccgcct
     2221 tcttcgccgt cggtgcctac acccaggcga tgctggtcac caagacgggg acgccgtggt
     2281 tcgcggccgc cgggttcgcc gtgctggccg cgatgctcgt cgcccttctc gtcggcctgc
     2341 cactgctccg gctccgaggg cacttcctgg ccctcgccac actcgggctc gggatcatcc
     2401 tctcggtgat cctgcgcgag ctcgaggtca ccgggggcac gtcggggatc ttcggcatcc
     2461 ccaaaccaga gctcggtggc cgggtctacg acagtccgca ggagttcttc tggttcatga
     2521 cccctatcgt cgtgctcggg ctgatgatgg ccgccaacct cgtccgcagc cgcacgggcc
     2581 gggcgctggg tgccgtcaac gactccgagg tcgctgcgga gtgtctcggc gtcgacacct
     2641 tcgcccttcg gctgcgggtc ttcgtcctcg ccggcgggta tgccgggctc gccggcgcct
     2701 gctatgcgca ctgggtgtcc gtcgtcagcc cggaggtgtc cggcttcggc ctgagcgtcc
     2761 gcatgctgct catcgtcgtg ctgggcggtc tgggctcggt ctggggagcg gtcacgggcg
     2821 ccgtggccgt cgagatcctc gacgagggtc tgcgcaccgt catcccgaag ctcattcccg
     2881 gagcggccgg ggaggtgcag ctgctcggct tcggggtcat cctcgtcctg ctcgtcgtcc
     2941 tccttcccgg ggggctggac cagctgtggc aggcgctgac cgcgggcatc aggcgggtga
     3001 ggaaccaggt cgcgtcggcc gccgcactgc cgcccgtcga cgaccagggc catgacgtcg
     3061 ccctcgcgac gctgctcggc gaggcccagg cacccgaacg ggggagcctt gtcctgcggg
     3121 tacgtggggt ggcgaagcac tacggcgggg tcaaggcgct cgctgacct
//
LOCUS       DATAIX010000361         3072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_168873, whole genome shotgun sequence.
ACCESSION   DATAIX010000361 DATAIX010000000
VERSION     DATAIX010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168873"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VHM65_05395"
     CDS             complement(<1..215)
                     /locus_tag="VHM65_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705173.1"
                     /translation="MRLPSFLASSSASAGPTGLVFRSPTGAPFRHSNFYRRIFRPAVA
                     VAGLPASLRFHDLRHTCVALLIAQGAHP"
     gene            complement(336..560)
                     /locus_tag="VHM65_05400"
     CDS             complement(336..560)
                     /locus_tag="VHM65_05400"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX2705174.1"
                     /translation="MGEKGDQVKGKVKEAVGSLTGDKDLESEGKADRRAGEAKEKLGQ
                     AKDKVEDVVDKAEDKAEAVVDKAKDAMHRK"
     gene            838..1290
                     /locus_tag="VHM65_05405"
     CDS             838..1290
                     /locus_tag="VHM65_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705175.1"
                     /translation="MTEIWIWTVWEQVPERLESAAESPEETTEEGPPPDLAGFSVDAS
                     DGHIGEIDEANFDAGSGCIIVDTGFWIFGKKRMVPAGMIEKIDTESKKVVLSCSKADV
                     KAAPDYDPERVDDPGHRDEVGQHYEGVRDLPVGPEAEKARAEAKGPRG"
     gene            1487..1693
                     /locus_tag="VHM65_05410"
     CDS             1487..1693
                     /locus_tag="VHM65_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705176.1"
                     /translation="MGTGVVVIVVAVAVVLVVLLVTLSMRDRQKRDAQARRDRDEAHE
                     RAGRAERDRDIAQEQVEPRTDPDQ"
     gene            complement(1997..2854)
                     /locus_tag="VHM65_05415"
     CDS             complement(1997..2854)
                     /locus_tag="VHM65_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705177.1"
                     /translation="MTEPGSPEVAVREPGRRRHHRARIVGAIVGVALIAGVATLVVLW
                     NRGSSRPVSMEEARRRAGAPGTDQSTGAVPFRPAAGIYRYRGEGTEHLDRPPLTQTQG
                     PDIPGTVTHLEGRCWRLRVDYSTNHWQSWDYCPAASGLTENAGAFFQRLDLVVAKVDT
                     SSTYTCDPPVDAIRATQRAGDHWMQECRGTSTGSDGEVFSSGPYTYVGPERLDIGGTK
                     VAALHYHRLRNLTGGQTGTEDVNVWFDAATGLPVRNQRVITVHSSSLIGGVTYEENGS
                     FQLASMNPT"
     gene            complement(2847..>3072)
                     /locus_tag="VHM65_05420"
     CDS             complement(2847..>3072)
                     /locus_tag="VHM65_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705178.1"
                     /translation="ARSWRHQVDLDGDGTGIEPYVLPVPLPAERRNVDTDFEHLAGRL
                     RCVVRERGQVVFEGTSELAGLEVGSLPGRD"
BASE COUNT          500 a         1030 c         1047 g          495 t
ORIGIN      
        1 gggtgggcgc cctgggcgat gagcagcgcg acacacgtgt gacgaaggtc gtggaatcgc
       61 aggctcgcgg ggagaccggc cacggcgact gccgggcgga agatccggcg gtagaagttc
      121 gagtgacgaa acggcgcgcc cgtgggggag cggaacacca gcccggtcgg gccggcgctc
      181 gccgaggagc tcgcaaggaa gctcgggagt cgcaccgtgc gacggcagga ggtcttggtc
      241 gagccgagca cgaactcgcc acgcgagcgt ccccgaacgc cacttcggga acagcccgct
      301 cggctgtggc gaacgatctg gacctctcgg gccgattact tccgatgcat cgcgtccttc
      361 gccttgtcga ctaccgcttc ggccttgtcc tcggccttgt cgaccacgtc ttcgaccttg
      421 tccttcgcct ggccgagctt ctccttggcc tcaccagcgc gacgatcagc cttgccttcg
      481 gattccagat ccttatcgcc cgtgagactg ccgacggcct ccttgacctt ccccttcacc
      541 tgatcaccct tctcacccat cgtgcaccac cttccctcgt catggaactt gccgttgcgc
      601 ccggatcggc agggagcacc tccgactttg gccgggaacg ccggaactcg gtagaggcaa
      661 gagtccttac gccttgtacg cgagcccggc gccaacagtc tcgaactccg gcgcgggccg
      721 gtgtggcgct acgcgacagc gctcaacgcg gaccttttcc tctagcgagc cagcggtgca
      781 ggttgccaga gtcggagcag tgcgtcagga ccgcacgcga gagtcgggag cccatcgatg
      841 accgagatct ggatctggac cgtgtgggag caggttcccg agcgtctgga gagcgctgcc
      901 gagagtcccg aggagacgac cgaggagggc ccgccgcccg acctcgccgg tttctccgtc
      961 gacgcgagcg acggtcacat cggcgagatc gacgaggcca acttcgacgc cggaagcggc
     1021 tgcatcatcg tcgacaccgg cttctggatc ttcggcaaga agcggatggt cccggccggg
     1081 atgatcgaga agatcgacac cgagtcgaag aaggtcgtcc tctcctgcag taaggcggac
     1141 gtcaaggccg cccccgacta cgaccccgag cgcgtcgatg atccgggaca tcgcgacgag
     1201 gtcggccagc actacgaggg tgtgcgagac ctcccggtcg gaccggaagc cgagaaggct
     1261 cgcgcggaag ccaagggtcc acgaggttga gcagcgtagt cagcaagact gagcctgaga
     1321 ccgactgaaa ggcggctcga tatgtacatc ggagtgggaa cactcgtggt gatcctgatc
     1381 gtcgtgttga tcgtctacct cgttcgccgc tcctagcccc cacaactgcc tgggcatcga
     1441 ggagagggac gaagttttga gcactgccct tggtgtcgta cttgcggtgg gcaccggtgt
     1501 ggtcgtgatc gttgtcgcgg ttgcggtcgt gctcgtggtg ctgttggtga ccctgtcgat
     1561 gcgcgaccgc cagaagcgtg acgcccaggc tcggcgtgac cgggacgaag ctcacgagcg
     1621 cgcggggcgc gcggaacggg atcgcgatat cgcccaggag caggttgagc cgcgaactga
     1681 tccagaccag tagtccggcg caaccactcg cgcacggagc gtcgccacga gatcgtgacg
     1741 tacagatcga tggacccgag ttgtctcgtc ggcgtcggtt cggggacgca ccaactcgtc
     1801 ccgcggggac aactcagcct caacccccac cactcgaggg ggcagcaacg acctgtgggt
     1861 gtcggaagaa ctcgtctggg cctgtgatct gctgattcgc gtggccggtc gcggcccttg
     1921 gtgggaggct cggcggatgg cacgcatggt ttgagcgggc cccacatccc ggggctcaga
     1981 tcccggggca ccacacctag gtcgggttca tcgacgcgag ctggaagctg ccgttctcct
     2041 cgtaggtcac cccaccgatc agggaactgc tgtgcacggt gatgacccgc tggttccgca
     2101 cgggcaggcc ggtcgcggcg tcgaaccaca cgttgacgtc ctccgtgccg gtctggccgc
     2161 cagtcaggtt ccgcaaccgg tggtagtgca gggccgccac cttcgtacct ccgatgtcga
     2221 ggcgctccgg gccgacatag gtgtacgggc cggaggagaa cacctcgccg tccgaccctg
     2281 tcgaggtgcc acggcactcc tgcatccagt ggtcgcccgc tcgttgcgtc gccctgatgg
     2341 cgtcgaccgg tgggtcacac gtgtaggtcg aggaggtgtc gaccttggcc acgaccaggt
     2401 ccaaccgctg gaagaaagcg ccggcgttct ccgtgagccc cgaagcggct gggcagtagt
     2461 cccagctctg ccaatggttc gtgctgtagt cgacccggag cctccagcac cggccctcga
     2521 ggtgggtcac ggtgcccggg atgtcggggc cctgagtctg ggtcagcggc ggcctgtcga
     2581 ggtgctcggt gccctcaccg cggtaccgat agataccggc cgccggccgg aacggcaccg
     2641 cgccggtcga ctggtcggtg cccggcgctc cggcccggcg gcgggcctcc tccatcgaca
     2701 ccggccggga ggagccccgg ttccacagca cgaccaacgt cgccacaccc gcgatcaggg
     2761 cgacccccac gatggccccg acgattcggg cccgatggtg gcgacgccgc ccgggttcgc
     2821 gcaccgccac ctcgggggag ccgggctcag tcacggcccg gcaggctccc cacctcgagg
     2881 ccggccagct cggacgtgcc ctcgaacacc acctggccgc gctcacgcac tacgcagcgg
     2941 agccgaccgg ccaggtgctc gaagtcggtg tcgacgttgc gtcgctcggc cgggagcggg
     3001 acgggcagca cgtatggttc gatgccggtg ccgtccccgt ccaggtcgac ctggtggcgc
     3061 cacgaccggg cc
//
LOCUS       DATAIX010000362         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_168906, whole genome shotgun sequence.
ACCESSION   DATAIX010000362 DATAIX010000000
VERSION     DATAIX010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168906"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="VHM65_05425"
     CDS             <1..1196
                     /locus_tag="VHM65_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX2705179.1"
                     /translation="ASAGEWQPCRVRGEGERVVAVDAFTDFAVVTLRSHGLTALRILT
                     YDATGPAVFGAGWDVVPDEDLYTIGLGANLESATRRLQVVVESMVTPHSIYDYDVDER
                     TWTLLKRQPVLGGYDPGAYRQHRAWVTASDGTRVPVSVVHRADVAPDGTNPGWLYGYG
                     AYEASMDPYFSVARLSMLDRGVVCAVAHVRGGGEMGRSWYDAGRLMAKNTTFTDFLAA
                     ADLLVDQGWVAADRLVAEGGSAGGLLIGAAVNLAPHRFAAVHAAVPFVDPLTTILDPS
                     LPLTVTEWEEWGDPLHDPDVYRYLKSYAPYENIAAAPYPAILATTSLHDTRVHVVEPA
                     KWVARLRAVTVTDESRRPILLKTEMEGGHGGRSGRYDAWRQAAFELAFLLDAVGAVDI
                     VDARA"
     gene            1289..>2010
                     /locus_tag="VHM65_05430"
     CDS             1289..>2010
                     /locus_tag="VHM65_05430"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX2705180.1"
                     /translation="MMSRTDLRGATLTRSTVRAALPRAEFDVEAALLRVRPICEAVRS
                     QGATALADLSERFDGVRPARLRVPAPVLAAALAQLDPGLRDALDEAIRRVRLVHADQR
                     RTDTTTQVVPGGSVTERWVPVDRVGLYVPGGLAVYPSSVIMNVVPAQLARVGSLAVAS
                     PPQRENVGVFAGYPNVTILATCALLGVDEVYAVGGAQAVAMFAYGFTDDSDPAGVVVC
                     EPVSLVTGPGNIYVVAAKRLLKG"
BASE COUNT          259 a          732 c          709 g          310 t
ORIGIN      
        1 gcgcgagcgc cggcgagtgg cagccgtgcc gagtccgcgg cgagggcgag cgcgtcgttg
       61 ccgtcgacgc gtttaccgac ttcgcggtgg tcaccttgcg cagccatggg ctcaccgcgc
      121 tacggatcct cacgtatgac gcgaccggcc cagccgtctt cggcgccggc tgggacgtcg
      181 tccccgacga ggacctgtac acgatcgggc tcggtgccaa cctcgagtcc gcgacgcgtc
      241 ggctccaggt cgtcgtcgag tcgatggtga ccccgcacag catctacgac tacgacgtcg
      301 acgagcgcac ctggaccctg ctcaaacgcc agcccgttct cggcggctac gaccccggtg
      361 cctaccggca gcaccgcgcg tgggtcacgg cgtcggacgg gacgagggtt ccggtctcgg
      421 ttgtccaccg cgccgatgtc gcgccggacg gcaccaaccc cggatggctc tacgggtacg
      481 gtgcgtacga ggcctcgatg gacccgtact tctcggtcgc ccggctctcg atgctcgacc
      541 ggggagtggt ctgtgccgtc gctcacgtcc gcggtggcgg cgagatgggg cggtcctggt
      601 acgacgccgg gcgactcatg gccaagaaca cgaccttcac cgacttcctc gcggccgccg
      661 acctgctcgt cgaccagggg tgggtcgctg cggatcgtct tgtcgctgag ggaggttcgg
      721 ccggggggct gctcatcggg gccgcggtca acctcgcacc gcaccgcttc gcggccgtgc
      781 acgcagcggt tccgttcgtc gacccgctga ccacgatcct cgacccgtcg ctgccgctca
      841 cggtgaccga gtgggaggag tggggcgacc cgctgcacga ccccgacgtc taccgctatc
      901 tcaagagcta tgcgccatac gagaacatcg ccgcggcgcc atacccggcc atcctcgcga
      961 cgacgagcct gcacgatacc cgggtccacg tcgtcgaacc ggccaagtgg gtcgcgcggc
     1021 tcagggcggt cacggtgacg gacgagtccc gtcgcccgat cctgctcaag acggagatgg
     1081 agggcgggca cggcgggcga tctggccggt atgacgcgtg gcgacaggcc gcgttcgagc
     1141 tcgcgtttct cctcgacgcg gtgggcgcgg tcgacatcgt cgacgccagg gcgtgaggac
     1201 tgcctcctcg ccaccgctcg cccggcctcg gcgggcgggc aggtgagcac gccgggtccc
     1261 ggccttcggc ccccctagac ttgaggtcat gatgtcccgc accgacctgc gtggcgccac
     1321 cctgacccgc tccacggtgc gcgcggcgct gccccgggcc gagttcgatg tggaagcggc
     1381 gctcctgcgg gtgaggccga tctgcgaggc cgtacgcagc cagggagcca ctgcgctggc
     1441 cgacctctcg gagcggttcg acggtgtgcg gcccgcccgg ctgcgggtcc cggcgccggt
     1501 tctggcggcg gctctggccc agctcgaccc gggacttcgc gacgcgctcg acgaggcgat
     1561 ccggcgggtc cggctcgtcc acgccgacca gcggcgcacc gacacgacga cccaggtcgt
     1621 cccgggcggc tcggtcaccg agcggtgggt gcccgtcgac cgggtcggtc tctacgtccc
     1681 tggcggcctc gcggtctacc cgagcagcgt catcatgaac gtcgtccccg cccagctggc
     1741 ccgggtcggc tcgctcgcgg tggcctcccc gcctcagcgg gagaacgtcg gcgtcttcgc
     1801 gggctacccc aacgtcacga tcctggccac ctgtgccctg ctcggggtcg acgaggtcta
     1861 tgccgtcggc ggcgcccagg cggtggcgat gttcgcctac gggttcaccg acgactccga
     1921 cccagccggc gtcgtggtgt gcgagccggt cagcctggtc accggcccgg gcaacatcta
     1981 tgtcgtcgcg gccaagcggc tgctcaaggg
//
LOCUS       DATAIX010000363         2781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_49492, whole genome shotgun sequence.
ACCESSION   DATAIX010000363 DATAIX010000000
VERSION     DATAIX010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49492"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VHM65_05435"
     CDS             complement(<1..282)
                     /locus_tag="VHM65_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705181.1"
                     /translation="MSHRITSLLVVPAALAALALGAAPSVSQARPLSEPTGADACITH
                     TDSVSSARAAQSGNRRDGNELTEAQVKAFEAATAGAMTAKGLTTNGRGQA"
     gene            852..2105
                     /locus_tag="VHM65_05440"
     CDS             852..2105
                     /locus_tag="VHM65_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705182.1"
                     /translation="MAAPTRHLIGLLLGAEEDWPRAFEAILGMVGPVRDRRGVAHELT
                     SERLRIHPFNLAEPVRAELVIDRLAYWYYHPREWLKKASLMDGTYLLNSPFTFQSMEK
                     HSAYCAMLRLGLKIPSTVLVPYKHPLDNARWAYTSSKYNESFDLDAIAGELGYPMYMK
                     PFDGGGWRGVSRINNSDDLHRAYNESGEMLMHLQATVDYDHFARALSIGPETMVMDFR
                     PDEPMHNRYAVSHSFLSEAAGEQCVAISRIVNAFFGWEFNSAEMLVKDDDVYPIDYAN
                     ACPDVAVTSLHYYFPWAITALVRWSAYCLVTRRRQRIDLQTERYFAIADDTLMSYDEK
                     LRGYLEIADEHFETAAYWDWCDDFLPGLEDRVLEWVSGPDFDTLVRETVAATYPEHER
                     ATFLSHFRGLTGMWVTDHGGTPYAF"
     gene            complement(2140..>2781)
                     /locus_tag="VHM65_05445"
     CDS             complement(2140..>2781)
                     /locus_tag="VHM65_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016079909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2705183.1"
                     /translation="GEGPDAFCSGGDQRVRSDTGYLTDPGSSGGVGRFHVTDLHIQIR
                     RLPKPVVAMVAGYAIGGGHVLHLVCDLTIAADNARFGQVGPKVGSFDGGFGAGLLAQL
                     VGVKKAKEIWFLCRQYDAEQAREMGLVNAVVPLADLETETVGWCREMLAMSPLALRLM
                     KASFNAAEDGLSGIQQLAHDANLLFYSTDEAKEGREAYKAKRRPEFERFPRRP"
BASE COUNT          445 a          975 c          921 g          440 t
ORIGIN      
        1 tgcctgccct cgcccgttgg tcgtgaggcc cttggcggtc atggccccag cggtcgccgc
       61 ctcgaatgcc ttgacctgcg cctcggtcag ctcgttgccg tccctgcggt tgccgctctg
      121 ggcagctcgc gcggagctga ccgagtcggt gtgggtgatg caggcatcgg ccccggtggg
      181 ctcgctgagc ggcctcgcct ggctgacgga cggggcagcg ccgagggcca gtgcggccag
      241 cgctgccgga acgaccaaca aagacgtgat gcggtgcgac atgaggcttc cattcccatg
      301 cgccatggtc tggcgcaacc cctcgcacgg tagaccggct gggggccgga cgctgtgcta
      361 tttgcccaaa tccgggcgaa gaccttgagc ccgggaggaa ctctcaggcc gttgtcgagg
      421 cgacacgccg atcgacccaa gaattcctca accagggctt gcgcgcgtcc cggaaggggc
      481 gtagacagtt atcacgccct tccgcaagga agagcggtcg gacggcaaac gaggtccatc
      541 accgagggag caatcccgag ggagtgccgg gacgggcacg aggactcggc gcgctgaccg
      601 gcgttcggga gcgtcttcgc ccggacacct cgagacccgg ccaactcata cttggccggg
      661 tctcaccatt tatacccact gcccggcgac gaaccggtga ccgacacgcg gcgcgcacac
      721 ggcgggcacg ctgacattgt cgcgcggaca ttgtcgcgct cgggttggca ggtgggctgg
      781 ggctcccgct cgcgccggac gggcggtacg gtcgggccat cgactcgccc accggatgac
      841 agggagggac cgtggctgcc ccgactcgcc acctcatcgg actgctgctc ggcgcggagg
      901 aggactggcc gcgggccttc gaggcgatcc tgggcatggt cgggccggtg cgggaccgcc
      961 ggggcgtcgc ccacgagctc acctcggagc ggctcaggat ccacccgttc aacctggccg
     1021 agccggtgcg cgccgagctg gtcatcgacc ggctggccta ctggtactac cacccccgcg
     1081 agtggctcaa gaaggcctcg ctcatggacg gcacctacct gctcaactcc ccgttcacgt
     1141 tccagtcgat ggagaagcac tcggcctact gcgcgatgct ccggctcggg ctcaagatcc
     1201 cctcgaccgt cctcgtgccc tacaaacacc ccctcgacaa cgcccgctgg gcctacacgt
     1261 cctcgaagta caacgagtcc ttcgacctcg acgcgatcgc cggcgagctg ggctacccga
     1321 tgtacatgaa gccgttcgac ggcggcggct ggcgcggggt ctcgcgaatc aacaactccg
     1381 acgacctgca tcgcgcctac aacgagtccg gcgagatgct catgcacctg caggcgacgg
     1441 tcgactacga ccacttcgcg cgggccctgt cgatcgggcc cgagacgatg gtcatggact
     1501 tccggcccga cgaaccgatg cacaaccggt atgccgtgtc gcactcgttc ctgtccgagg
     1561 ctgcgggcga gcagtgtgtc gcgatctcgc gcatcgtcaa cgccttcttc gggtgggagt
     1621 tcaactcggc cgagatgctc gtcaaggacg acgacgtcta ccccatcgac tacgccaacg
     1681 cctgtcccga tgtcgcagtg acctcgctgc actactactt cccgtgggcg atcaccgccc
     1741 tggtgaggtg gtcggcatac tgcctcgtca cccggcggcg ccagcgcatc gacctgcaga
     1801 ccgagcggta cttcgccatc gccgacgaca ccctcatgtc gtatgacgag aagctccgcg
     1861 gctatctcga gatcgccgac gagcacttcg agacggccgc gtactgggac tggtgcgacg
     1921 acttcctgcc gggtctggag gaccgggtcc tggagtgggt cagcggcccc gacttcgaca
     1981 cgctggtgcg cgagacggtc gctgcgacat acccggagca cgagcgggcg acgttcctgt
     2041 cccacttccg tggcctgacc ggcatgtggg tgaccgacca cggcgggacg ccctacgcct
     2101 tctgagggcg accgcccgcc accaatccat ctccctgagt cagggacgcc gggggaagcg
     2161 ctcgaactcc ggacgccgct tggccttgta ggcctcccgc ccctccttgg cctcatcggt
     2221 ggagtagaac aacaggttgg cgtcgtgggc cagctgctgg atgccggaga ggccgtcctc
     2281 ggcggcgttg aagctggcct tcatcaaccg cagggcgagc ggcgacatcg ccagcatctc
     2341 gcggcaccac ccgaccgtct cggtctccag gtcggcgagg ggcacgaccg cgttgaccag
     2401 ccccatctcg cgagcctgct cggcgtcgta ctggcggcac aggaaccaga tctccttggc
     2461 cttcttgacc ccgacgagct gggcgagcag cccggccccg aagccaccgt cgaacgaccc
     2521 gaccttcggt cccacctgac cgaaccgggc gttgtccgcc gcgatggtga ggtcgcagac
     2581 gaggtgcagg acgtgtccac cgccgatggc gtagccggcc accatggcca ccaccggctt
     2641 gggcagccgg cggatctgga tgtgcaggtc ggtgacgtgg aagcgcccga ctccaccgga
     2701 gctgcccggg tcggtgaggt agccggtgtc gctgcggacc cgctggtcac cgcccgagca
     2761 gaaggcgtcg gggccctcac c
//
LOCUS       DATAIX010000364         6434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_19464, whole genome shotgun sequence.
ACCESSION   DATAIX010000364 DATAIX010000000
VERSION     DATAIX010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19464"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VHM65_05450"
     CDS             complement(<1..911)
                     /locus_tag="VHM65_05450"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX2705184.1"
                     /translation="MAATRQPATRHPVTWKPAFALIGAALLACLVAAAATRQAVQAPV
                     GLSDPGALVRWGLPVTRVAHDLAAALMIGMLVLAATVVPDDKSLPRVRVALRFASVAG
                     GAWVAAGLIGVVLGFASIAGTPVTSPEFGAQLQTFVWSLEPLRAQIVSTALVLVATSI
                     AAIATTRPGTALAAAVALVALLPLALAGHASGSADHETSVNALAVHLLAATVWVGGLA
                     GIAILRPRLHPHLPVVVRRYSTIALVCFVALGVSGVLSAWLRIGSWAGLATSYGAVLL
                     VKVGAFVLLGAAGWTQRRRVVEALGRTP"
     gene            955..1797
                     /locus_tag="VHM65_05455"
     CDS             955..1797
                     /locus_tag="VHM65_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEX2705185.1"
                     /translation="MSDPDATAMTVPALVDAFAQTVGAVIALGRSCSPADFELPTDCP
                     GWTVHDQIAHVAGVESFLHGEPQPVVEVPDYAYLRHDIGRFIEAGVEARRGVPGPAVV
                     DELAALLPKRLAVLRDPALTARTPLRGLLGERPAEGVLRIRVTDVWVHEQDLREALGL
                     PGGMDTAAAAVFVGLVLDSAPRIISRGAAVPAGQTVTIEVTGPVTASTSVAVVTAEDG
                     KPRGDVVARPAAGSPGTTLISLSARDLTRRGAGRIAREATTYTVVGDEAVAARVLDEL
                     AVTP"
     gene            1885..2397
                     /locus_tag="VHM65_05460"
     CDS             1885..2397
                     /locus_tag="VHM65_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2705186.1"
                     /translation="MAIGQDTADEISENTIRILKLLGSLRHLVPRVHPELDPSSYPSL
                     FALSAGPQRVSDLAGCVHSDVSTASRQVSALVSLGLAQKVPDPHDRRVQLVGLTPDGT
                     TVIERLQDSRRIWFQAMLATWSEADGADFASYLTRFVADLETFRADALGRPEHAHRTP
                     AGWISQTATT"
     gene            2405..4138
                     /locus_tag="VHM65_05465"
     CDS             2405..4138
                     /locus_tag="VHM65_05465"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX2705187.1"
                     /translation="MASATTPAHQAPAAGGPLTHRQIVTILIGLMMGMFLAALDQTIV
                     ASAMRVIADDLKGLSAQAWVTTAYLITSTVVTPLYGKLSDIYGRKQFFILAISLFTIG
                     SVLCTLATSIGTLALYRAVQGLGAGGLFSLALAIIGDIVPPRERAKYQGYFLAVFGTS
                     SVLGPVIGGFFAGQDSILGVTGWRWVFLVNVPIAIAALFVVTRTLHLHHVRLDHRIDY
                     WGAVALTVALVPLLIVAEQGREWGWGSGNALLCYGVGLAGFVAGLLVERRMGEEALIP
                     LRLFQNRTVAVSSIASVFIGMGMFGGLACLPLYLQIVKGASATQAGLQLLPMTLGIMF
                     GSIFSGQMISRTGKYRQYPIIGAGLLVGALFAFHYIGADTPLWRSMIIMVFFGVGLGF
                     NFQPLTLAVQNAVSPRDIGVATSSATFTRQIGGTLGTAVFLSILFSKAAENIPARYAQ
                     AAGSGDFQSAMASPAGDPAANAAFLAQLKAGQSGGSTSGFAAALSDSSFVSVIDPRLA
                     HPYLVGFAESMSTVFLVASGVLVLAFAVSWFLPHVELRSNADFAQSRAEHAASGGTPA
                     EAASAPPPLGH"
     gene            complement(4135..5343)
                     /locus_tag="VHM65_05470"
     CDS             complement(4135..5343)
                     /locus_tag="VHM65_05470"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2705188.1"
                     /translation="MLSAYRPLFRVPGVLPFMAGGALARVGGAMFGVSVIVMISSRRG
                     SYSLAGAVSAVAIAVLAVAGPVIGRLVDKHGQRRVVVPFVIVSSACGYATVALSFAGA
                     PAWTLFASYGLSALLPEMGPLSRARWAHIYRDDRDTLHTAMSFEQVFDEASFVLGPVL
                     AVILSTLWFPEAGVLVAEIAYTLGMLLFVSARATEPPVVPHHQRPGGLAASRPGMLVV
                     ATILVMTGVILGANEVVAVAVAEKGGDKGFSSVILGLFAAGSTLAGLLYGARRFRSLT
                     SRRMLYAALGMFVLEAPALLVHDLRGIALVMLVAGSATAPMLITSLTLSQLLVPAALI
                     TEGMAVALTGILIGISIGAAVSGVAIERLGAHDAYAVPVTAGLLAAALAALSHRRLAR
                     AERAALAQAI"
     gene            complement(5504..>6434)
                     /locus_tag="VHM65_05475"
     CDS             complement(5504..>6434)
                     /locus_tag="VHM65_05475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX2705189.1"
                     /translation="AQPAAGPRPLAKPPVRKYAKDRGVDLSEVAGSGPDGVVTRDDID
                     AHVAGGPAPAAAASGPAYGMAARPSSGERETREPIKGVRKMMAAAMVGSAFSAPHVTE
                     FTTVDVTATMDLVARLKGDREFTDVKVTPLLVLAKAMCLAVRRNPLVNATWDEAAQEV
                     VVKHYVNLGIAAATPRGLLVPNIKDADQMGMRELGEAIGALTATAREGRTPPADMAGG
                     TITITNVGVFGVDTGTPIINPGEAAILAFGVVRRMPWVLTSADGSESIEPRWVTQLAL
                     SFDHRLVDGALGSRFIADVAAILHDPARALVWG"
BASE COUNT          934 a         2331 c         2268 g          901 t
ORIGIN      
        1 ggcgtgcggc cgagcgcctc gacgacgcgc cggcgctggg tccatccggc ggcaccgagc
       61 aggacgaagg cacccacctt gacgagcaga accgccccgt atgacgtcgc gagccccgcc
      121 cacgagccga tccgcagcca ggcactgagg acacccgaca cgccgagggc gacgaagcag
      181 acgagtgcga tcgtcgagta gcgccggacc acgaccggca gatgcgggtg caggcgcggg
      241 cgcaggatgg cgatgccggc gagcccgccg acccacacgg tggcggcgag caggtgcacc
      301 gcgagggcgt tgaccgaggt ctcgtggtcg gcgctgccgg aggcgtgacc ggccagcgcg
      361 agtggcagca gggcgacgag ggcgacggcc gccgccaggg cggtgccggg gcgggtcgtc
      421 gcgatcgcgg cgatggaggt ggcgacgagc accagggcgg tgctgacgat ctgggcgcgc
      481 agcggctcca gggaccagac gaaggtctgg agctgagcgc cgaactcggg gctcgtcacg
      541 ggggtgcccg cgatcgaggc gaacccgaga acgacgccga tcaggccggc cgcaacccag
      601 gcccctccgg cgaccgaggc gaagcgcagc gcgacgcgga cccgaggcag ggacttgtcg
      661 tcagggacca cggtcgctgc gaggacgagc atcccgatca tgagcgccgc tgcgaggtcg
      721 tgggcgaccc gggtgaccgg caggccccag cgcacgagcg caccaggatc ggacagcccg
      781 accggagcct ggaccgcctg ccgggtcgcc gcggcggcca cgaggcacgc gagcagggcc
      841 gcgccgatca gggcgaaggc gggcttccag gtcacgggat gccgggtcgc gggttgccgg
      901 gtcgctgcca cgtgtccacc ctatggctgc gcacgggcgc cgtagagtgg ctcgatgagt
      961 gatccagacg ccactgccat gacggtcccc gcgctcgtcg acgccttcgc ccagaccgtg
     1021 ggcgccgtca tcgccctcgg caggtcctgc tcaccggccg acttcgagct ccccacggac
     1081 tgtcccgggt ggaccgtgca cgaccagatc gcccatgtgg ccggggtcga gtccttcctg
     1141 cacggtgaac cccagccggt cgtcgaggtg ccggactacg cctacctgcg ccacgacatc
     1201 ggccgcttca tcgaggccgg ggtcgaggcc cgtcggggcg tgccgggccc ggcagttgtc
     1261 gacgagctcg ccgccctgct gccgaagcgg ctggccgtcc tgcgcgatcc ggcgctgacc
     1321 gcacggactc ccctccgtgg gctgctcggt gagcggccgg ccgagggggt cctccgcatc
     1381 cgggtcaccg acgtgtgggt gcacgagcaa gacctgcgcg aggcgctcgg gctccccggc
     1441 ggcatggaca ccgcggccgc ggcggtcttc gtcgggctgg tgctggactc ggcgccgcgg
     1501 atcatctccc gcggtgctgc ggtgccggcc ggtcagaccg tcacgatcga ggtgaccggc
     1561 ccggtgacgg cgtccacgtc ggttgccgtc gtgaccgcgg aggatggcaa gccccgcggc
     1621 gacgtcgtcg ccaggccggc cgcaggatct ccgggcacga cgctcatctc gctctcggcg
     1681 cgcgacctga cccgccgggg cgccgggcgg atcgcgcggg aggcgacgac atacaccgtc
     1741 gtgggagacg aggcggttgc ggctcgagtg ctcgacgagc tcgccgtcac gccgtagccc
     1801 ctcctcccag gcgggaatat gcgcccgtgg gcgagccttt gcactagttg cgtgcttcac
     1861 acaactactg atggggaggg tggtatggcg atcgggcagg acacggccga cgagatcagc
     1921 gagaacacca tccgcatcct caagctcctc ggctcgctgc gccacctcgt gccgcgggtg
     1981 catccggagc tcgacccgag cagctacccc tcgttgttcg ccctgtccgc cggaccgcag
     2041 cgggtgtcgg acctcgccgg gtgcgtgcac tcggacgtgt ccaccgcgag ccggcaggtg
     2101 agcgccctgg tgtccctcgg cctggcccag aaggtgccgg acccgcacga ccgccgggtc
     2161 cagctggtcg ggctcacccc ggacgggacc acggtcatcg agcgactgca ggactctcgc
     2221 cggatctggt tccaggccat gctggccacg tggtccgaag cggacggggc ggacttcgcg
     2281 tcatacctga cccgcttcgt cgccgacctc gagacgttcc gggccgacgc gctcggcagg
     2341 cctgagcacg cccaccgcac ccccgcgggc tggatctccc agaccgccac gacctaggag
     2401 caccatggcc tccgccacca ctccggcaca ccaggccccc gccgccggcg ggccactgac
     2461 ccaccggcag atcgtcacga tcctcatcgg gctcatgatg gggatgttcc tcgccgcgct
     2521 cgaccagacg atcgtcgcct ccgccatgcg agtcatcgcc gacgacctca agggcctgtc
     2581 cgcccaggcg tgggtgacga cggcctacct catcacctcg accgtcgtca cacccctgta
     2641 cggcaagctc tcggacatct acgggcgcaa gcagttcttc atcctcgcca tctcgctctt
     2701 cacgatcggc tcggtgctgt gcacactggc gacctcgatc gggacgctgg cgctctaccg
     2761 ggcggtccag ggcctcggcg ccggcgggct gttctcgctg gccctggcga tcatcggcga
     2821 catcgtgccg ccccgtgagc gcgcgaagta ccagggctac ttcctcgcgg tgttcgggac
     2881 gtcgagcgtg ctgggcccgg tcatcggcgg gttcttcgcg gggcaggact cgatcctcgg
     2941 ggtcaccggc tggcgctggg tcttcctggt caacgtcccg atcgcgatcg ctgccctgtt
     3001 cgtcgtgacg cgcaccctgc acctgcacca cgtccggctc gaccaccgga tcgactactg
     3061 gggtgcggtg gcgctgaccg tggcactcgt gccgctgctc atcgtcgccg agcagggccg
     3121 ggagtggggc tggggctccg gcaatgccct gctctgctac ggcgtcgggc tggccgggtt
     3181 cgtcgccggg ctgctggtcg agcgccggat gggcgaggag gccctgatcc cgctgcgcct
     3241 ctttcagaac cgcaccgtcg cggtgtcctc gatcgcgtcg gtcttcatcg ggatggggat
     3301 gttcggtggc ctggcctgcc tgcccctcta cctgcagatc gtcaagggcg cgtcggcgac
     3361 ccaggccggc cttcagctgc tgccgatgac cctcgggatc atgttcggct cgatcttctc
     3421 gggccagatg atctcccgga cgggcaagta ccgccagtac ccgatcatcg gggccgggct
     3481 cctcgtcggc gcgctcttcg ccttccacta catcggcgcg gacaccccgc tgtggcggtc
     3541 catgatcatc atggtgttct tcggcgtcgg gctcggcttc aacttccagc cgctcaccct
     3601 cgcggtgcag aacgcggtat ccccgcgcga catcggagtg gcgacctcgt cggcgacctt
     3661 cacgcggcag atcggtggga ccctcggcac cgccgtcttc ctgtcgatcc tgttctccaa
     3721 ggccgccgag aacatcccgg cccgctacgc ccaggccgcc ggctcgggcg acttccagtc
     3781 ggccatggcc tcacccgccg gggacccggc ggcgaacgcc gccttcctgg cccagctcaa
     3841 ggccggtcag tccggcggct cgacctcggg cttcgcggct gccctgagtg actcgtcctt
     3901 cgtctcggtc atcgaccctc gcctcgccca cccctacctc gtggggttcg ccgagtcgat
     3961 gtcgacggtc ttcctcgtgg cctcgggcgt gctggtcctc gccttcgccg tgtcgtggtt
     4021 cctgccgcac gtcgagctgc gcagcaacgc cgacttcgcc cagagccggg ccgagcatgc
     4081 tgcatcggga ggcaccccgg cggaggccgc ctcggctccg ccgccgctcg gccactagat
     4141 ggcttgggcc agtgccgcgc gctcggcccg ggccaggcgc cggtgcgaca gcgcggccag
     4201 ggccgccgcc agcagacccg cggtgaccgg cacggcatac gcgtcgtggg ccccgagccg
     4261 ctcgatcgcc acgccgctca ccgcggcgcc gatcgagatc ccgatgagga tgccggtcag
     4321 cgcgacggcc atgccttcgg tgatgagggc ggcgggcacg agcagctggc tcaaggtgag
     4381 gctggtgatg agcatcgggg ccgtggccga gccggcgacg agcatgacga gcgcgatccc
     4441 gcgcaggtcg tggacgagca gcgccggggc ttcgaggacg aacatcccga gcgctgcgta
     4501 cagcatgcgc cggctcgtca ggctgcggaa cctgcgcgct ccatagagga gcccggccaa
     4561 ggtcgacccg gcggcgaaca gcccgaggat cacactcgag aaccccttgt ccccaccctt
     4621 ctcggcgacc gcgaccgcca cgacctcgtt ggcgccgagg atcactccgg tcatgacgag
     4681 gatcgtcgcg acgacgagca tgcccggtcg tgaggcggcg aggccaccgg ggcgctggtg
     4741 gtgcggcacc acgggcggct cggtcgctcg ggccgagacg aacaagagca tgccgagggt
     4801 gtaggcgatc tcggccacga ggaccccggc ctcggggaac cacagggtcg acaagatgac
     4861 cgccaggacc gggccgagca cgaaggacgc ctcgtcgaag acctgctcga acgacatcgc
     4921 cgtgtgcagc gtgtcccggt cgtcccggta gatgtgggcc cagcgggcgc gggacagcgg
     4981 ccccatctcg ggaagcaagg cggacagccc gtatgacgcg aacagggtcc aggccggtgc
     5041 gcccgcgaac gacagggcca ccgtggcgta cccgcaggcc gaggagacga tgacgaaggg
     5101 cacgaccaca cgccgctgcc cgtgcttgtc gacgagccgt ccgatgacgg ggcccgccac
     5161 ggccagcacc gcgatggcga ccgccgacac ggccccggcg aggctgtagg agcctcgccg
     5221 cgaggagatc atgacgatga cactgacccc gaacatcgcc ccgccgaccc gggccagcgc
     5281 tccgcctgcc atgaacggca gaactcccgg tacgcggaag aggggacggt aggcggacag
     5341 cacggtagga ctcctgggca acggggaaca caccattctc ggccaccggc aaccgtgggc
     5401 caaacccccc ggctcacaac caccacatca ccgagggtga cctcgttgcg gtgggcgagc
     5461 ccggctcacg accaccacat caccgagggt gacctccttg cgatcagccc cagacgaggg
     5521 ctctcgccgg gtcgtgcagg atcgcggcga cgtcggcgat gaagcgcgac ccgagcgcgc
     5581 cgtcgacgag ccggtggtcg aaggacaggg ccagctgggt cacccatcgt ggctcgatcg
     5641 actcggagcc atcggcactg gtgaggaccc acggcatccg gcgcacgacc ccgaacgcga
     5701 ggatcgccgc ctcgcccggg ttgatgatcg gcgtccccgt gtcgacgccg aacaccccga
     5761 cgttggtgat ggtgatcgtc ccgccggcca tgtccgccgg gggggtccgg ccctcacggg
     5821 ccgtcgcggt gagcgcgccg atggcctcgc ccagctcacg catccccatc tggtcggcgt
     5881 ccttgatgtt gggcacgagc agcccgcgcg gggtcgccgc ggcgatcccg aggttgacgt
     5941 agtgcttgac cacgacctcc tgggcggcct cgtcccaagt cgcgttgacg agcgggttgc
     6001 gtctcaccgc gaggcacatc gccttggcca gcacgagcag cggcgtcacc ttgacgtccg
     6061 tgaactcacg gtcgcccttg agccgcgcga cgagatccat cgtggcggtg acgtcgacgg
     6121 tggtgaactc ggtgacatgc ggggccgaga acgccgaccc gaccatggcc gccgccatca
     6181 tcttgcgcac acccttgatc ggctcgcggg tctcccgctc ccccgacgat gggcgggccg
     6241 ccataccgta tgccggtccg ctcgcggcgg ctgcgggggc aggcccgccg gccacatgag
     6301 cgtcgatgtc gtcgcgggtg acgacaccgt ccggacccga cccggcgacc tcggacaggt
     6361 cgacaccacg gtccttggcg tacttgcgca ccggcggctt ggccagcggg cgagggcccg
     6421 cggcgggctg ggcg
//
LOCUS       DATAIX010000365         2908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_276784, whole genome shotgun sequence.
ACCESSION   DATAIX010000365 DATAIX010000000
VERSION     DATAIX010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276784"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VHM65_05480"
     CDS             complement(<1..670)
                     /locus_tag="VHM65_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX2705190.1"
                     /translation="MTLAARASMPVPASPLTGLGPDRREAFELRVQRWYDDIESGLAD
                     VYGEAAAPALADELVALAAGAYADRDPELHRLDTRRLLEPDWLQSPRMFGYAAYADRF
                     AGDLAGVADRVPYLHDLGVTYLHLMPLLNPREGDNDGGYAVQDYRSVRPDLGTVEDLR
                     RLATTLRDNGISLVLDVVLNHVAREHDWAVRARAGDPAYREYFYVFPDRAVPDAYERS
                     LPEVF"
     gene            complement(721..2835)
                     /locus_tag="VHM65_05485"
     CDS             complement(721..2835)
                     /locus_tag="VHM65_05485"
                     /inference="COORDINATES: protein motif:HMM:NF009377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-like protein EF-G2"
                     /protein_id="HEX2705191.1"
                     /translation="MSRSNAKGAASIAPPEPSSPQQIRNVVLVGPGGAGKSTLVDYLV
                     SARVPGRRPHEGAHERSVSLAVASLGSNGLVINLIDTPGFPDFVGELRAGVRAADAAI
                     FVISAADDVDGATALLWQECASVGMPRAVVVTKLDAARSDYDETIATCQRVFGDAQAL
                     YLPVYGPGKTVVGTMGLLSQNVYDYTSGERVTRAPSADEAARIETRRGALMEAIIEES
                     EDDTLLDRYLEGEDIEIDTVIADLRTAVARGMFFPIVPSSPQTGAGVEEIFELIEQAF
                     PDPTQHPLPTLTSPVGAALPAMTCDPDGPLVAEVIRTSSDPYVGRLSLVRVFSGTLRP
                     DETVHVSGHLEHFVGRRVEGHGDHDDEERVGPLSSAVLDDTHHRAKAIAGEVVLITKL
                     SRAETSDTLSAKDRPALVEPWVLPEPLLPMAIHAASKGDEDKLAGALQRLATEDVTMR
                     MEHNAETHQVVMWTMGQAHVEELVTRLKDRYGVSVEAEPFRTALRETFVRKCEAQGRH
                     VKQSGGHGQYAVCRVAIEPLPRGSGFEFVDKVVGGAVPRQFIPSVEKGIVAQLDKGVL
                     AGYPLVDIRVTLYDGKAHSVDSSDMAFQTAGALALKEAANESTVSLLEPIDVVSVTVS
                     DDFVGAVMSDLRTRRGHVLGTEPAATAARTIVRAEVPQTELARYAIDLRSVSHGTGIF
                     SRQQLRYDYMPADLAKGYLKDA"
BASE COUNT          413 a          986 c         1069 g          440 t
ORIGIN      
        1 ggaacacctc gggcaggctc cgctcgtagg cgtcgggcac cgcgcggtcg ggaaagacgt
       61 agaagtactc ccggtatgcc gggtcgccgg cccgcgcgcg gaccgcccag tcatgctcgc
      121 gggcaacgtg gttgagaacg acgtcgagga cgagtgatat cccgttgtcc cggagcgttg
      181 tggccagccg gcgcagatcc tcgaccgtgc ccaggtcggg ccggaccgag cggtagtcct
      241 gcacggcata cccgccgtcg ttgtcgccct cgcgggggtt gagcaacggc atgaggtgca
      301 ggtaggtcac tccgaggtcg tgcaggtacg ggacccggtc ggccaccccg gccaggtcgc
      361 cggcgaaccg gtcggcgtac gcggcatacc cgaacatgcg cggagactgg agccagtccg
      421 gttcgagcag ccggcgagtg tcgagccggt gcagctcggg gtcgcggtcg gcgtacgccc
      481 ccgctgcgag cgcgacgagc tcgtccgcga gtgcaggtgc cgcagcctcg ccatacacgt
      541 cggcaaggcc cgactcgatg tcgtcgtacc agcgctgcac gcgcagctcg aacgcctccc
      601 ggcggtccgg gccaagaccc gtgagcgggc tggccgggac gggcatactc gcacgtgccg
      661 cgagggtcat gggctgattc aaccgcagcc ccaggctcgt ctgccacacc agtcgcgcgg
      721 tcaggcgtcc ttgaggtagc ccttcgcgag gtcggccggc atgtagtcgt agcgcagctg
      781 ctggcggctg aagatgcccg tgccgtgtga caccgagcgc aggtcgatgg cgtagcgcgc
      841 cagctcggtc tgcggtacct cggcccgcac gatcgtgcgc gccgccgtgg cggccggctc
      901 ggtgccgagg acatggccgc gacgcgtgcg caggtcggac atgaccgccc cgacgaagtc
      961 gtcggacact gtcacgctga ccacgtcgat cggctcgagc agcgacaccg tcgactcgtt
     1021 ggccgcctcc ttgagcgcca gcgccccggc ggtctggaac gccatgtcgg acgagtcgac
     1081 cgagtgcgcc ttcccgtcgt agagggtgac cctgatgtcg acgagcgggt agcccgcgag
     1141 cacgcccttg tcgagctggg cgacgatgcc cttctcgacc gaggggatga actggcgcgg
     1201 cacggcgccc ccgacgacct tgtcgacgaa ctcgaacccg ctcccgcgcg gcagcggctc
     1261 gatcgcgacc cggcacacgg cgtactgacc gtggcctccg ctctgcttga cgtggcgccc
     1321 ctgcgcctca cacttgcgca cgaacgtctc gcgcagggcc gtgcggaagg gctcggcctc
     1381 gacggacacg ccgtagcggt ccttgagccg cgtgacgagc tcctcgacgt gggcctggcc
     1441 catcgtccac atgacgacct ggtgggtctc ggcgttgtgc tccatccgca tcgtgacgtc
     1501 ctcggtcgcg aggcgctgca gggcgccggc gagcttgtcc tcgtcgccct tggaggccgc
     1561 gtggatcgcc atgggcagca gcggctcggg caggacccac ggctcgacca gcgccggacg
     1621 gtccttggcc gagagcgtgt cggaggtctc ggccctgctc agcttggtga tgagcacgac
     1681 ctcaccggcg atggccttgg cccggtggtg ggtgtcgtcg agcaccgcgc tggacagcgg
     1741 gcccacccgc tcctcgtcgt cgtggtcgcc atggccttcg acgcggcgtc cgacgaaatg
     1801 ctcgagatgg ccggagacat ggaccgtctc gtcgggtcgc agcgtgccgc tgaagacgcg
     1861 cacgaggctg agccgaccga catacgggtc gctgctcgtg cggatgacct cggcgaccaa
     1921 cggcccgtcg gggtcgcacg tcatggcggg cagggccgcc ccgacgggcg aggtgagtgt
     1981 gggcagcggg tgctgcgtcg gatcggggaa cgcctgctcg atgagctcga agatctcctc
     2041 gacgcccgcg cccgtctgcg gtgaggacgg cacgatgggg aagaacatgc cgcgcgcgac
     2101 cgccgtcctg aggtcggcga tgaccgtgtc gatctcgatg tcctcgccct cgagatagcg
     2161 gtcgagcagg gtgtcgtcct cggactcctc gatgatcgcc tccatgaggg caccgcggcg
     2221 cgtctcgatc cgagcggcct cgtctgcact cggggcgcgg gtgacccgct cgccgctggt
     2281 gtagtcgtag acgttctggg agagcaggcc catcgtgccg acgacggtct tgcccggccc
     2341 gtagacgggc aggtagagag cctgggcgtc gccgaagaca cgctggcagg tggcgatcgt
     2401 ctcgtcgtag tcggagcggg cggcgtcgag cttggtcacg acgacggcgc ggggcatccc
     2461 gacggaggcg cactcctgcc acagcagggc agtggcgccg tcgacgtcgt cggccgcgga
     2521 gatgacgaag atcgccgcgt cggccgcccg cacgcccgcc cgcagctcac cgacgaagtc
     2581 ggggaacccg ggggtgtcga tgaggttgat gacgaggccg ttgctcccga ggctcgcgac
     2641 ggcgagcgac acggatcgct cgtgggcacc ctcgtggggg cgacgaccag ggacccgggc
     2701 ggatacgagg tagtcgacga gggtcgactt ccctgcacct ccgggtccca cgaggacgac
     2761 gttgcggatc tgctgggggc tgctgggctc gggaggcgcg atggacgcgg ctcccttggc
     2821 attcgatcgg ctcacgccct tacctttcac cccgcgcggg tcgtcgcaca agcccactcg
     2881 ctcgggtgcc ggggtgctgg gggtgccg
//
LOCUS       DATAIX010000366         3899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_217117, whole genome shotgun sequence.
ACCESSION   DATAIX010000366 DATAIX010000000
VERSION     DATAIX010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217117"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..861)
                     /locus_tag="VHM65_05490"
     CDS             complement(277..861)
                     /locus_tag="VHM65_05490"
                     /inference="COORDINATES: protein motif:HMM:NF037152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX2705192.1"
                     /translation="MADAALSAEAILDAAEGVIRRFGPAKATVLDVARSLGVSHGSVY
                     RHFPSKVALRDAVAQRWLERVSQPLREIAAANAPAPARLRAWLEALSSTKQRMAREDP
                     ELFATFHRITTESREVVTAHLDLLAAQLSRIVADGVASGHYTVTDPHAAGRAVLHATA
                     RFHHPAHAIEWADPRLAGDFDAVASLVARGLAAA"
     gene            complement(865..1887)
                     /locus_tag="VHM65_05495"
     CDS             complement(865..1887)
                     /locus_tag="VHM65_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2705193.1"
                     /translation="MNPPSPLGARSLGEDGPGVSALGLGLMGMSDLYGPADDAESIAT
                     IHAALDAGVTLLDTGDFYGAGHNELLLRDALHGRDRDSVTISVKFGVLRDPSGGWNGI
                     DARPVAVKNALAYSLRRLGTDHVDIYRPARLDPNVPVEETVGALAELVEAGFVRHIGL
                     SEVGAETLRRAQAVHPISDVQIEYSLISRGIEDTLLPTARELGVGITAYGVLSRGLLS
                     GHWSTDRAQAPEQSQDFRRHLPRFSGENLTANLRLVEALRAIATARGATVAQVAIGWA
                     LARGEDIVPLVGARTRDRLAESLGAVRLELTPDDLASIEAAVPADAVAGTRYDPGQMS
                     MLDSER"
     gene            2151..2531
                     /locus_tag="VHM65_05500"
     CDS             2151..2531
                     /locus_tag="VHM65_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEX2705194.1"
                     /translation="MANERRPASVYGVGAEPDPRFTLANERTTLAWIRTALALVAGGV
                     ALISFTSLAPNLPAWAPLIGTASCAGGGLLAIRSIQRWAAVERAMRRREPLPPPKALF
                     VLAIGVIVLAVMVLGLAVFELAVR"
     gene            2528..2869
                     /locus_tag="VHM65_05505"
     CDS             2528..2869
                     /locus_tag="VHM65_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEX2705195.1"
                     /translation="MSIPGRHALQPERTALAWSRTAVIANLTLVPLLLVNLQIGVWPL
                     VVTGALVLIAASLVTLRLRHRYVQLRDDTTGYSPFGAIARLAAVVGATAATGVVTGVV
                     MAVRAGTFGRT"
     gene            complement(2752..>3899)
                     /locus_tag="VHM65_05510"
     CDS             complement(2752..>3899)
                     /locus_tag="VHM65_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705196.1"
                     /translation="HAWPEQRDTRWDATFARVCTSQGVSVDRGLGAFEELTRESLAAG
                     SPWPSPQLSTLSAGQPVEYSAGGRPGVRFTTNVGDPVLAPHARVRSGLAAVGRTADLL
                     GVAQGWALAQPLVQLLTDPALPVPDGSRFWLWAGADLAVDRPPALKAYLSLHAGDIDG
                     WPTRRVAALRAAGIPERGTVWAALACLRTACWGHEIGVGMGPDGRWGIKVYDELDRWR
                     PDVLAHLLAACRLRGRVGDLAPEIPGVLRATTTTRRRAGIAIRVDPESGDVTEVTTAA
                     AFPTPLVAREVLTGRVLAWLQASSRPSDPLRALAAAVAPEWEGAPAGARMLALFTRTI
                     GRRGASTTVYVRPNVPARTAITTPVTTPVAAVAPTTAASLAMAPNGE"
BASE COUNT          519 a         1401 c         1447 g          532 t
ORIGIN      
        1 ggccgggacc ggccgccgag gtccgcccgg tgcacctcgc cctgcccgtc ctcgtcggcg
       61 tcatcggcac ggccgcgacg gccgcctggt ggcggcgagc cgaggtcgtc taggaccccg
      121 ggccgtagtc gccaccgcgc tgagatgccc cgactggttg tccggaccag tccaactggc
      181 cgaacggccg tcttgtgtcc acagtgcccc ggtatgacct gatcctgaca acgttggtcc
      241 tgttcgggtg ccgcaaagac gggtagggaa tacgcgctat gccgcggcga gccctcgcgc
      301 gacgagggag gcgacggcgt cgaagtcgcc ggccagcctc gggtcggccc actcgatggc
      361 gtgcgccgga tgatggaagc gagccgtcgc atgcaggacc gcccgcccgg cggcatgcgg
      421 atcggtgacc gtgtagtgac cggaagccac cccgtcagcg acgatccggc tcagctgggc
      481 cgcgagcagg tcgaggtggg ccgtgacgac ctcccgggac tcggtggtga tccgatggaa
      541 ggtggcgaag agctcagggt cctcgcgggc catccgttgc ttcgtcgacg agagcgcctc
      601 aagccaggcg cgcagccgag ccggtgccgg agcgttggcg gcggcgatct cgcgaagtgg
      661 ctgcgagacc cgctcgagcc agcgctgcgc cacggcatcg cgcagcgcga ccttgctcgg
      721 gaagtggcgg tagacgctcc cgtggctcac gccgaggctg cgggcgacgt cgaggacggt
      781 ggccttggcc gggccgaacc gcctgatgac cccttcggcg gcgtcgagga tcgcctcggc
      841 gctgagcgcg gcatcggcca agggtcaccg ctcgctgtcg agcatcgaca tctggcccgg
      901 gtcgtacctg gtcccggcca ccgcatccgc gggcaccgcg gcctcgatcg acgcgaggtc
      961 gtccggggtg agctcgaggc ggaccgcgcc gagcgactcg gcgagccggt ctcgggtcct
     1021 ggcccccacg aggggcacga tgtcctcgcc gcgcgcgagg gcccagccga tcgccacctg
     1081 ggcgaccgtc gcgccccgag cggtcgcgat ggctcgcagt gcctcgacga gccgcaggtt
     1141 cgccgtgagg ttctccccgg agaagcgcgg caggtgccgg cggaagtcct gtgactgctc
     1201 cggagcctgc gcccggtcgg tgctccagtg gccggacagc aggccccgcg acagcacgcc
     1261 atacgcggtg atgccgacgc cgagctcgcg cgctgtcggc agcagcgtgt cctcgatgcc
     1321 gcgggagatc agcgagtact cgatctgcac gtcgctgatc gggtgcacgg cctgcgcgcg
     1381 gcgcagcgtc tcggcgccga cctcggacag gccgatgtgc cggacgaacc ctgcctcgac
     1441 gagctcggcg agagcgccga cggtctcctc gacaggcacg ttcgggtcga gccgggccgg
     1501 ccggtagatg tcgacgtggt cggtgccgag ccggcgcagc gagtaggcga gcgcgttctt
     1561 gaccgcgacg ggacgggcgt cgatcccgtt ccagccgccg gacgggtcgc gcagcacgcc
     1621 gaacttgacg ctgatcgtca ccgagtcccg gtcgcgcccg tgcagcgcgt cgcgcaggag
     1681 aagctcgttg tgtccggctc cgtagaagtc gccggtgtcc aggagggtga cgcccgcgtc
     1741 gagcgcggcg tggatcgtcg cgatgctttc ggcgtcgtcg gcgggaccgt agaggtccga
     1801 catgcccatg agaccaagtc cgagagcgga gacgcccggg ccgtcctcgc cgagcgaacg
     1861 ggcaccaagg ggtgatggcg ggttcatgca gaggagccta cccggtcaac tgacagaatc
     1921 gcaactctgt cagttgactc catcggcagc acgggacgcc gccgacctgg gcgggcctgg
     1981 atcggggact cccgaactgg ccaaagcgcg ccatacgaga tggatttgac cgggtttatg
     2041 gcgatcttgg gggcaatccc cggcgtttgg ccagtccggc tgttcggccg gacggtgcca
     2101 cgggcgtcac ggggcccgca gccagcagct cgggccgtac cctggccact gtggcgaacg
     2161 agcgcagacc tgcgagcgtg tatggcgtcg gcgcggagcc ggacccccgg ttcaccctcg
     2221 ccaatgagcg cacgacgctg gcgtggatcc gcaccgccct cgccctcgtg gcgggcgggg
     2281 tcgccctcat ctcgttcacc tcgctcgcgc cgaacctgcc ggcctgggcg ccgctgatcg
     2341 gcaccgcgtc ctgtgcgggg ggtgggcttc tcgccatccg ctcgatccag cgctgggccg
     2401 cggtggagcg tgcgatgcgt cgcagggagc ctctcccacc accgaaagcg ttgttcgtgc
     2461 tggcgatcgg cgtcatcgtc ctcgccgtca tggtgctcgg tctcgcggtg ttcgagctgg
     2521 ctgtccggtg agcatcccgg ggaggcacgc gctccagccg gaacgcaccg ctctggcgtg
     2581 gtcacgcacg gcggtcatcg ccaacctcac cctcgtcccg ctcctgctcg tcaacctcca
     2641 gatcggagtc tggccgctcg tggtcaccgg cgccctcgtc ctgattgccg cgagcctggt
     2701 cacgctgcgc ctgcgccacc gctacgtcca gctgcgcgac gacacgacgg gctactcccc
     2761 attcggcgcc atcgcgagac tcgccgcggt ggtcggcgcc acggcggcga ccggcgtggt
     2821 caccggcgtg gtgatggcgg tccgggccgg cacgttcggc cgcacgtaga cggtcgtcga
     2881 cgcgccacgc cggccgatcg tgcgggtgaa cagggcgagc atcctggcac cggccggcgc
     2941 gccctcccac tccggggcga cggcggcggc gagcgcgcgc agcgggtcac tcggccggct
     3001 gctcgcctgc agccaggcga ggacccggcc cgtgagcacc tcgcgggcga cgagcggggt
     3061 ggggaaggcg gccgcggtcg tgacctcggt gacatcaccg gactccgggt ccacccggat
     3121 ggcgatgccg gcccggcgcc gcgtcgtcgt ggtcgctcgc agcacaccgg ggatctccgg
     3181 tgccaggtcg ccgacccgtc cgcgcagccg gcaggccgcg agcaggtggg cgaggacgtc
     3241 gggacgccac cggtcgagct cgtcgtacac cttgatcccc cagcgcccgt cgggacccat
     3301 gccgaccccg atctcgtggc cccagcacgc cgtgcgcagg cacgcgagcg cggcccacac
     3361 cgtccctcgc tcggggatcc cggccgcccg cagcgcggcc acccgacggg tgggccagcc
     3421 gtcgatgtct cccgcatgca ggctgaggta ggccttgagg gccggcgggc ggtcgacggc
     3481 gagatcggcg cccgcccaga gccagaaccg gctcccgtcg ggcacgggca gcgcgggatc
     3541 ggtgaggagc tgcacgagtg gctgcgcgag ggcccacccc tgcgccacgc ccagcaggtc
     3601 ggcggtgcgc cccaccgcgg cgagcccgct gcggacgcgc gcgtgcgggg caaggaccgg
     3661 gtcgcccacg ttcgtcgtga agcgcacccc gggccgcccg cccgcggagt actccacggg
     3721 ctggccggcc gacagcgtcg acagctgggg cgagggccac ggcgagcccg ccgccaggct
     3781 ctcgcgggtc agctcctcga aggcgccgag ccctcggtcg accgagaccc cttgggaggt
     3841 gcacacccgg gcgaaggtcg cgtcccagcg ggtgtcgcgc tgttcgggcc aggcatggg
//
LOCUS       DATAIX010000367         2340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_49792, whole genome shotgun sequence.
ACCESSION   DATAIX010000367 DATAIX010000000
VERSION     DATAIX010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49792"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VHM65_05515"
     CDS             <1..519
                     /locus_tag="VHM65_05515"
                     /inference="COORDINATES: protein motif:HMM:NF008528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEX2705197.1"
                     /translation="GHMAPPVGSAPRAPRARRRRWPALVFVLVLVVPVAEIAVIIGVG
                     RVIGGWPTVVLLLVESALGAWLVRREGTRTWGALSTALRTGQMPSRQLADAALVLIGG
                     TLLLTPGFVTDLVGFFFIVPLTRPLARAALEAFVARRLLGGIASVPGPSGSRGPQPPS
                     AASGDVIEGEIL"
     gene            complement(639..998)
                     /locus_tag="VHM65_05520"
     CDS             complement(639..998)
                     /locus_tag="VHM65_05520"
                     /inference="COORDINATES: protein motif:HMM:NF024789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HEX2705198.1"
                     /translation="MADRSLRGTRLGGFSMESDENVVPSERQITTYVCPQGHRIELPF
                     SVEADIPATWDCRCGADARLLDGPEPVAKNVKPVRTHWDMLLERRSIPELEELLEERL
                     TLLRESRGEKRRTKRTA"
     gene            complement(1145..>2340)
                     /locus_tag="VHM65_05525"
     CDS             complement(1145..>2340)
                     /locus_tag="VHM65_05525"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2705199.1"
                     /translation="AHVSLLGVGISPGALVPFTLTASTIISAVVLILVGAVADRSPRP
                     TRLFAAFAWTGSLAASLLFLVSGTSWALGIVLLIIANLCLGASLVIYDSLLCRIASPD
                     ERDRVSSRGWALGYAGGGLLLALNFGLLSFADSLGISDGTAVRISMLSAGIWWAAFTL
                     IPVLGLRDVQGTTATPVEVRRGVVGGSFTQLATTFKELRAYPQTLLFLLAYLFYNDGI
                     QTVIGSSSLYGTSELGFTQDKVLMVFLLVQFVAFGGALLFGRLAGRYGAWRTVLGGIA
                     MWLVTVVVAFVTPGRQLGLFAMLGVLIGIVMGGTQALSRSLFSQLIPQGREAEFFSLY
                     QAMERGTSWLGTLVFGIVYQLTLSYRSAIIALIVFFVVGGLLLSRLKMREGIIAAGNE
                     VPSVI"
BASE COUNT          376 a          805 c          823 g          336 t
ORIGIN      
        1 ggccacatgg cgccaccggt agggtcggct ccccgcgctc cccgcgctcg ccggcggcgc
       61 tggcccgcgc tcgtcttcgt cctggtcctc gtggtgcccg tcgccgagat cgccgtcatc
      121 atcggggtcg gcagggtcat cggtgggtgg cccacggttg tcctgctcct cgtcgagtcg
      181 gctctgggcg cgtggctcgt ccggcgcgag ggcacccgga cgtggggcgc cttgtcgacc
      241 gcgctgcgca ccgggcagat gccgtctcgc cagcttgccg acgcggcgct ggtcctcatc
      301 ggggggaccc tcctgctgac acccgggttc gtgaccgacc tggtcgggtt cttcttcatc
      361 gtgccgctca cccggccgct cgcccgggcc gcgctggagg ccttcgtggc tcggcgtctt
      421 ctgggcggga tcgcctccgt gcccggcccg tcgggctccc gtggcccaca gcccccgtcg
      481 gcagcgtccg gggacgtcat cgagggcgag atcctctagc accggccagc acccctggcc
      541 ggcccgggcc gccgccgcag agcagcgtca tcgcacacac agccgggccc gcacctccac
      601 aggaggtgcg ggcccggctg ttgcggtcgg gttggtggtc aggccgtgcg cttggtccgg
      661 cgcttctcgc cccgggactc gcgcaggagc gtcaggcgtt cctccaggag ctcctcgagc
      721 tcgggaatcg agcggcgctc gagcaacatg tcccagtggg tccggaccgg cttgacgttc
      781 ttggcgacgg gttctggccc gtcgagcagg cgcgcgtcgg cgccgcagcg gcagtcccac
      841 gtcgccggga tgtccgcttc gacgctgaag gggagctcga tgcggtgccc ctgcgggcac
      901 acataggtgg tgatctgacg ctcactagga acgacgttct catccgactc catgctgaag
      961 cccccgagcc gggtgccgcg caacgatcgg tcagccatga ggatcctccc tggtggattc
     1021 tcggcgcaca ggagatgcgc ccgatgcctc agaggttcaa acgacccggt cggttgcttt
     1081 gttccacgag aatgtgccgt aattcgcccc gcccaccacg cctcatcgcc gcccggggcg
     1141 cacctcagat gaccgatggt acctcgttgc cggccgcgat gatgccctcg cgcatcttga
     1201 gccgcgacaa gagcagcccg ccgacgacga agaagacgat gagggcgatg atggccgaac
     1261 ggtaggacag ggtgagctgg tagacgatcc cgaagacgag ggtgccgagc cagctcgtcc
     1321 cacgttccat ggcctggtag aggctgaaga actccgcctc acggccctgg gggatcagct
     1381 ggctgaacag cgaccgggac agcgcctgcg tgccacccat gacgatgccg atgaggacgc
     1441 cgagcatcgc aaagagcccc agctgcctgc ccggcgtgac gaaggccacg accaccgtga
     1501 cgagccacat cgcgatcccg ccgaggacgg tgcgccacgc gccataccgg ccggcgagcc
     1561 tgccgaagag caacgcacct ccgaaggcga cgaactggac gagcaagaac accatgagga
     1621 ccttgtcctg ggtgaacccc agctcactgg tgccgtagag gctcgagctg ccgatgacgg
     1681 tctggatgcc gtcgttgtag aacaggtagg cgagcaggaa cagcagggtc tgcgggtagg
     1741 cccgcagttc cttgaacgtc gtcgcgagct gggtgaagct gccaccgacg acgccccggc
     1801 gtacctccac cggagtggcg gtcgtgccct ggacgtcgcg cagccccagc accgggatga
     1861 gggtgaaggc cgcccaccag atcccggcgc tgagcatgct gatccgcacc gccgtgccgt
     1921 cgctgatgcc gagggagtcc gcgaaggaga gcagcccgaa gttgagcgcg aggagcaggc
     1981 cgccgccggc atacccgagg gcccagcctc ggctcgacac ccggtcgcgc tcgtccggcg
     2041 aggcgatccg gcagagcagc gagtcataga tgacgagcga ggcaccgaga cacaggttgg
     2101 cgatgatgag gagcacgatc ccgagggccc agctggtccc tgagacgagg aacagcagcg
     2161 acgcggcgag cgaccccgtc cacgcaaagg ccgcgaagag acgggtcggg cgggggcttc
     2221 ggtcagcgac ggcccccacg aggatgagca cgaccgccga gatgatcgtc gaggcggtca
     2281 gggtgaaggg caccagggcg ccgggtgaga tcccgacacc gagcaggctc acgtgtgccg
//
LOCUS       DATAIX010000368         2995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_246970, whole genome shotgun sequence.
ACCESSION   DATAIX010000368 DATAIX010000000
VERSION     DATAIX010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246970"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VHM65_05530"
     CDS             <1..315
                     /locus_tag="VHM65_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2705200.1"
                     /translation="WPACEIAPWALAKAQTDVAMLEDDLITVPWPWREVTRGIAVPTL
                     LVTGDHEVIFDPGMLAQLAEVGNPLIETAVVPRAGHCVRRDQTEAYHRLVDAWIEGQL
                     AR"
     gene            complement(300..1232)
                     /locus_tag="VHM65_05535"
     CDS             complement(300..1232)
                     /locus_tag="VHM65_05535"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2705201.1"
                     /translation="MGVGLLTSSSHRASPIVSRMPDPTLSPRVRLHEWGTPRANRALL
                     VLLHGLTDSGPCWADAVARWAPAYQVIAWDARGHGESPRFSPGELETGVGLTHLADAV
                     AMLEGLAERGLPRPILVGHSMGGGTAAAVCASRPDLVRAAVLEDPALGFFRREARLGA
                     DSVKQDRIDEARRVRVDLDGAIRQGRLDFPGWPACEIAPWALAKAQTDVAMLEDDLIT
                     VPWPWREVTRGIAVPTLLVTGDHEVIFDPDLVMETESGDGSAGSLVQVIGQVPLRRIV
                     SIHVDGLEAAPDDELLWYDVTEFDTVAGLAHRAS"
     gene            1231..2859
                     /gene="pruA"
                     /locus_tag="VHM65_05540"
     CDS             1231..2859
                     /gene="pruA"
                     /locus_tag="VHM65_05540"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR01236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEX2705202.1"
                     /translation="MDAVTRTPSPVNEPIRDYAPGSPERSDLESALARLGHERIDLPS
                     TIGGAKVMGSGELVNVVQPHARGKVLGTMRDSTGADARTAVAAATAAAPMWRDLSFDD
                     RAAILLKAASLLAGPWRATLNAATMLGQSKTCYQAEIDSACELIDFWRLNVHFGRQIM
                     AEQPLFSSPGVWNRTDYRSLEGFVYAVTPFNFTAIGGNLPTAPALMGNTVVWKPAHSQ
                     QLAAHYTMLILEEAGLPPGVINLVTGHGPVVSEAVLADPGFAGMHFTGSTRVFQLLWR
                     RAAANLSSYRTYPRLVGETGGKDFILAHPSADPDVLRTSLIRGAFEYQGQKCSAASRA
                     YVPASLWKTIKADLAELTNGLPMGDVTDLSNFMGAVIDERAFAKHAGAIDRARASSTI
                     EILAGGTYDDAAGFFVRPTVLVGSDPSDEIFHTEYFGPILGIHVYPDRTYDTVLDQME
                     SVASYALTGSIIAQDRRAVADATHRLRFAAGNFYINDKPTGAVVGQQPFGGGRASGTN
                     DKAGAAANLMRWTSMRSIKETFVPPKDHRYAHMG"
BASE COUNT          457 a         1079 c         1038 g          421 t
ORIGIN      
        1 tggccggcct gcgagatcgc gccgtgggcg ctcgcgaagg cccagaccga cgtggcgatg
       61 ctcgaggacg acctcatcac cgtcccctgg ccgtggcgcg aggtcacgcg gggcatcgcc
      121 gtgcccacgc tgctcgtcac cggtgaccac gaggtgatct tcgaccccgg gatgctggcc
      181 cagctggccg aggtggggaa cccgctcatc gagacggcgg tcgtcccccg agccggacac
      241 tgcgtccgcc gggaccagac cgaggcctac catcgcctcg tggacgcctg gatcgagggt
      301 cagctcgcgc gatgagccag cccggcgacg gtgtcgaact cagtgacgtc gtaccagagc
      361 agctcgtcgt ccggtgccgc ctcgagccca tcgacgtgaa tcgacacgat gcgtcgcagc
      421 gggacctggc cgatgacctg gacgaggctg cccgccgacc cgtcgccgga ctcggtctcc
      481 atgacgaggt cggggtcgaa gatcacctcg tggtcaccgg tgacgagcag cgtgggtacg
      541 gcgatgcccc gcgtgacctc gcgccacggc caggggacgg tgatgaggtc gtcctcgagc
      601 atcgccacgt cggtctgggc cttcgcgagc gcccacggcg cgatctcgca ggccggccag
      661 ccggggaagt ccagccgccc ctgacggatg gcgccgtcga ggtcgacccg gacccggcgc
      721 gcctcgtcga tgcggtcctg cttgacgctg tcggcgccga ggcgcgcttc gcgacggaag
      781 aaccccagcg ccgggtcctc gagcaccgcc gcacggacga gatcgggccg ggaggcgcag
      841 acggcggctg ccgtcccgcc tcccatcgag tggcccacga ggatagggcg gggcagcccg
      901 cgctcggcaa ggccttcgag catcgccacg gcgtcggcga ggtgtgtgag gcccacgccc
      961 gtctccagct cgccggggga gaaccgtggc gactcgccat ggccgcgtgc gtcccaggcg
     1021 atgacctggt atgccggcgc ccaccgggca accgcgtcgg cccagcacgg ccccgagtcg
     1081 gtgaggccat gcaagagcac caggagtgcg cgattcgccc ggggcgtacc ccactcgtgc
     1141 aggcgaaccc tgggcgagag ggtcggatcc ggcatacgtg acacgatagg tgacgcccgg
     1201 tgagacgacg aggtcaggag cccgacaccc atggatgccg tcactcgcac tccctctccg
     1261 gtcaacgagc cgatccgcga ctacgcgcca ggcagcccgg agcgctctga cctcgagtcg
     1321 gcgctggctc gtctgggcca tgagcggatc gacctgccga gcacgatcgg cggcgccaag
     1381 gtcatgggta gcggcgagct ggtcaacgtc gtgcagccgc atgcccgggg caaggtcctc
     1441 gggacgatgc gcgactcgac cggcgccgac gcgcgcacgg cggtcgctgc cgccacggcg
     1501 gcggcgccga tgtggcgaga cctgtccttc gacgaccgtg cggcgatcct gctcaaggcc
     1561 gcaagcctgc tggccgggcc gtggcgggcg acgctcaacg cggcgacgat gctcggccag
     1621 tccaagacgt gctaccaggc cgagatcgac tcggcctgcg agctcatcga cttctggcgt
     1681 ctcaacgtcc acttcgggcg ccagatcatg gcggagcagc cactgttctc ctcgcccggt
     1741 gtgtggaacc gcaccgacta ccgctcgctc gagggcttcg tctacgcggt gacgccgttc
     1801 aacttcaccg cgatcggcgg caacctcccc acggctcccg cgctcatggg caacaccgtc
     1861 gtctggaagc cggcccacag ccagcagctc gccgcccact acacgatgct catcctcgag
     1921 gaggccgggc tgccgccggg cgtcatcaac ctcgtcaccg gccacggccc ggtcgtctcc
     1981 gaggccgtgc tcgccgaccc cggtttcgcc gggatgcact tcaccgggtc gacgcgcgtc
     2041 ttccagctgc tctggcggcg ggcggcggcc aacctctcgt cctaccggac ctatccgcgc
     2101 ctcgtcggcg agaccggagg caaggacttc atcctcgccc acccgtcggc cgacccggac
     2161 gtgctgcgga cgtcgctgat ccggggagcg ttcgagtacc aagggcagaa gtgctcggcc
     2221 gcatcccgcg cctatgtccc cgcgtcgctg tggaagacga tcaaggccga cctcgccgag
     2281 ctgacgaacg gcctgccgat gggcgacgtg accgacctgt ccaacttcat gggcgcggtc
     2341 atcgacgagc gggcgttcgc caaacatgcg ggggcgatcg accgcgcccg cgcgtcgagc
     2401 acgatcgaga tcctggccgg cggcacgtat gacgacgcgg ccggcttctt cgtgcgcccg
     2461 acagtcctcg tcggttcgga cccgagcgac gagatcttcc acaccgagta cttcggcccg
     2521 atcctcggga tccacgtcta cccggaccgg acctacgaca cggtgctcga ccagatggag
     2581 tcggtcgcgt catacgccct gaccggctcg atcatcgccc aggaccggcg ggccgttgcc
     2641 gacgcgaccc accggctgcg cttcgccgca ggcaacttct acatcaacga caagccgacg
     2701 ggcgcggtcg tcgggcagca gcccttcggc ggtggccggg cctcggggac gaacgacaag
     2761 gcgggcgccg cggcgaacct catgcggtgg acctcgatgc gctcgatcaa ggagaccttc
     2821 gtgccaccca aggaccaccg ctacgcacac atgggctgag cgagcctgcg ccgggcgcgg
     2881 ccgacggtct cagggcgtgt ctgggagctc gggtggcggg gaccatggcc gggccaggcg
     2941 gcgggacctt gtcaggatga tcgcgatgtg gaccgtgctc tcgtggttct cgggc
//
LOCUS       DATAIX010000369         1774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_79830, whole genome shotgun sequence.
ACCESSION   DATAIX010000369 DATAIX010000000
VERSION     DATAIX010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79830"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VHM65_05545"
     CDS             <1..984
                     /locus_tag="VHM65_05545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2705203.1"
                     /translation="VDPLDPRDERLVLGCIKVYNRANSRVNGPHAQQVNLDQIRSVAR
                     DPAERRRAWAALDEDGKVVGAVTLIEPLRENLDTASVWLAIRPRRWREGIGRLLQEQA
                     ESAAHASGRHRLYDHQESSDPSGGPGAAFARAMGHRESRLDLALELELPVPGRLLKAW
                     EHEVRHPAYEILTAWDTLPQDWLEDRARLARRLSTDAPLGIDLDEGDWDADRLRTQWA
                     RGREQGTRAVESVARERATGRLVAYSDVSVALGNPDLAIQLDTLVLREHRGHRLGMAV
                     KVANLRALAHELPGLARIRTWNAESNTPMLAVNQAMGFEVVGWTREWVKDL"
     gene            1007..>1774
                     /locus_tag="VHM65_05550"
     CDS             1007..>1774
                     /locus_tag="VHM65_05550"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2705204.1"
                     /translation="MTDAGGQGFSTRAIHAGQEADSRTGAVVPAIYQTSTFKQDGIGG
                     FREGYEYSRSANPTRTALEECLAALEGGSRGFAFASGLAGQDTVVRALLRPGDHVVVP
                     SDAYGGTYRYFAKVAAPWGLEHDIAAVADADAVRAAIRPGQTRLVWLETPTNPLLGIA
                     DIAAIAEIAHGAGALLVVDNTLATPYLQTPLALGADVVTHSTTKYAGGHSDVVGGAVI
                     VAHEVGVASYGDAVERIAFHQNSIGGVAGPFDSWLVLR"
BASE COUNT          263 a          645 c          625 g          241 t
ORIGIN      
        1 gtcgacccgc tcgacccccg agacgagcgg ctcgtgcttg gctgcatcaa ggtctacaac
       61 cgcgccaaca gccgggtcaa cggcccgcac gcgcagcagg tcaacctcga ccagatccgc
      121 tcggtggcgc gtgatcccgc cgagcggcgc cgggcgtggg cggccctgga cgaggacgga
      181 aaggtcgtcg gagcggtcac cctcatcgag cccctgcgag agaacctcga cacggccagc
      241 gtctggctcg ccatccgccc gagacggtgg cgcgagggga tcggccgcct gctccaggag
      301 caggccgagt cggcggccca cgcatccggt cgccaccggc tctacgacca ccaggagtcc
      361 agtgacccgt cgggtggtcc cggggcggcc tttgcccgcg cgatgggcca ccgggagagc
      421 cggctcgacc tggccctgga gctcgagctg cccgtgccgg gaagactgct caaggcctgg
      481 gagcacgagg tgcgccaccc ggcatacgag atcctcaccg cctgggacac cctgccgcag
      541 gactggctcg aggaccgcgc ccggctcgcg cggcggctca gtaccgacgc gccgctcggc
      601 atcgacctcg acgaagggga ctgggatgcc gaccggctcc ggacccagtg ggcccgcgga
      661 cgcgagcagg ggacccgggc ggtcgagtcg gtcgcgcgcg aacgggcgac ggggcggctc
      721 gtcgcctact ccgacgtctc ggtcgcgctg ggcaaccccg acctggccat ccagctggac
      781 acgctcgtcc tgcgcgagca ccgcggacac cggctcggta tggcggtcaa ggtcgccaac
      841 ctgcgcgcgc tggcgcacga gctgcccggc ctggcccgga tccggacgtg gaacgccgag
      901 tccaacacgc ccatgctggc ggtcaaccag gcgatgggct tcgaggtcgt cggctggacc
      961 cgtgagtggg tcaaggacct gtgacgagcg ggatagcctc tgggccatga ctgacgcagg
     1021 cggccagggc ttctcgacac gggcgatcca cgcgggccaa gaggccgact cccgcacggg
     1081 ggccgtggtc ccggccatct accagacgag taccttcaag caggacggga tcggcggctt
     1141 ccgcgaggga tacgagtact cccgctcggc gaaccccacc cgaaccgccc ttgaggagtg
     1201 tctcgccgcg ctcgaaggcg gttctcgcgg gttcgccttc gcgtcgggcc tggccggcca
     1261 ggacacggtg gtccgggccc tgctgcgccc cggcgaccac gtcgtcgtcc cctcggacgc
     1321 gtatggcggc acgtaccgct actttgccaa ggtcgccgct ccctggggcc tggagcacga
     1381 catcgccgcg gtggccgacg ccgacgccgt gcgggccgcg atccgccccg ggcagaccag
     1441 gctcgtctgg ctggagaccc cgaccaaccc tctgctcggc attgccgaca tcgcggccat
     1501 cgccgagatc gcccatgggg caggggcctt gctcgtcgtc gacaacacct tggcgacgcc
     1561 ctacctgcag accccgctcg cgctcggcgc cgacgtcgtc acccactcga cgacgaagta
     1621 cgccggcggt catagcgacg tcgtcggggg cgctgtcatc gtcgctcacg aggtgggcgt
     1681 tgcgtcatac ggggatgccg tagaacggat tgccttccac cagaactcga ttggcggggt
     1741 tgcggggccg ttcgactcgt ggctcgtcct gcga
//
LOCUS       DATAIX010000370         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_80163, whole genome shotgun sequence.
ACCESSION   DATAIX010000370 DATAIX010000000
VERSION     DATAIX010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80163"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /gene="rho"
                     /locus_tag="VHM65_05555"
     CDS             <1..887
                     /gene="rho"
                     /locus_tag="VHM65_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX2705205.1"
                     /translation="EFGKLVPLYPQERLRLETTPNVLATRIIDLVAPIGKGQRGLIVS
                     PPKAGKTLILQAIANAITTNNPEAHLMVVLVDERPEEVTDMQRTVKGEVIASTFDRPA
                     DDHTTVAELAIERAKRLVELGHDVVVLLDSITRLGRAYNLAAPASGRILSGGVDSSAL
                     YPPKRFFGAARNIENGGSLTILATALVETGSKMDEVIFEEFKGTGNMELRLSRQLADR
                     RIYPAVDVNPSGTRREEILMGPEELKVMWKLRRVLSALDSQQGIELLIDRLKKTKSNV
                     EFLRQVQMTSSIKLDDED"
     gene            complement(967..1542)
                     /locus_tag="VHM65_05560"
     CDS             complement(967..1542)
                     /locus_tag="VHM65_05560"
                     /inference="COORDINATES: protein motif:HMM:NF037405.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX2705206.1"
                     /translation="MFVHLAGQLSLVDQVVLGDSLVRRNAAALRDLRKFCSTYDGRAA
                     SAATAAATLVRCGVDSPMETRLRLLIVLAGLGEPTVDYRLHDEEGNLLYRVDLSFPQV
                     RLAIEYDGAHHDEVGQHGRDLRRREDLEAAGWTFIVVVSKHFHDTPESVLARIHRAMK
                     AAGLAVPTRLSERWRAHVFPRATVVTRPKSA"
BASE COUNT          345 a          718 c          696 g          311 t
ORIGIN      
        1 tcgagttcgg caagctcgtg ccgctctacc cgcaggaacg gctgcgcctg gagaccacgc
       61 cgaacgtcct ggcgacgcgc atcatcgacc tcgtggcgcc gatcggcaag ggccagcgtg
      121 ggctcatcgt ctcgcccccc aaggcgggca agacgctcat cctgcaggcc atcgccaacg
      181 cgatcacgac gaacaacccc gaggcacacc tcatggtggt cctcgtcgac gagcggcccg
      241 aagaggtcac cgacatgcag cgtacggtca agggtgaggt cattgcctcg accttcgacc
      301 gtcctgccga cgaccacacc acggtcgccg agctcgccat cgagcgggcc aaacgcctcg
      361 tcgagctggg ccacgacgtg gtcgttctcc tcgactcgat cacccgcctc gggcgcgcct
      421 acaacctggc cgccccggcc agcggccgca tcctgtcagg gggggtcgac tcctcggcgc
      481 tatacccgcc caaacggttc ttcggtgccg cccgcaacat cgagaacggt ggctcgctga
      541 cgatcctcgc cacggccctg gtcgagaccg ggtccaagat ggacgaggtc atcttcgagg
      601 agttcaaggg caccgggaac atggagctgc gcctgtcgcg ccagctcgcc gaccggcgga
      661 tctacccggc ggtcgacgtc aacccgtccg ggacccgccg cgaggagatc ctcatgggcc
      721 ccgaagagct caaggtcatg tggaagctgc gccgggtcct ctcggcgctc gactcccagc
      781 agggcatcga gctgctcatc gaccggctca agaagaccaa gagcaacgtc gagttcctcc
      841 gtcaggtcca gatgacctcc tcgatcaagc tcgacgacga ggactgaccc ccgcgccctt
      901 cgggcctggt caggcctgcg catccgctgg aagtcgcctc cggggagggt ttcatccggg
      961 ctgggctcag gcggacttcg gtcgcgtcac cacggtcgcg cgggggaaga catgtgcccg
     1021 ccagcgctcc gacagacgcg tggggacggc cagcccggct gccttcatgg cgcggtggat
     1081 gcgtgccaag acgctctcgg gcgtgtcgtg gaagtgcttg ctgaccacga cgatgaacgt
     1141 ccagccggct gcctccaagt cctcgcggcg gcggaggtca cgtccgtgct ggccgacctc
     1201 gtcgtggtgg gcgccgtcat actcgatcgc cagcctcacc tgcggaaagc tgaggtccac
     1261 ccggtagagg aggttgccct cctcgtcatg gagcctgtag tcgaccgtgg gttcgccaag
     1321 accggcgagg acgatgagca ggcggagtcg cgtctccatg ggtgagtcca ctccgcaccg
     1381 gaccagggtc gcggccgccg tggcagcgga cgcggcgcgg ccgtcatacg tgctgcagaa
     1441 cttccggaga tcgcggagtg ctgcggcgtt ccggcgcacc aggctgtctc cgagaacgac
     1501 ctggtcaacg aggctgagct ggccggccag gtgcacgaac atcagcgcag gggtggtgac
     1561 gcgccatggc gcactgcgct cgcgccatcc gtcccgaggc ggtgggttcg tagcccgacc
     1621 gtgccgaagc gtgagctccg tgggaccgcc acgtggatca ccgggtcgtc aggaacgact
     1681 ccgccccaga gccgggcagc tgtgccgtgc gagacgcgtg acccggcggg cgcgaccaac
     1741 agggcgcctt gcgcgcgtag ggccagcgtc agagggattg cagcatcgac atagacgccg
     1801 cggaacagcc tgcgaaaatg ctcccggcgc agatcactgt cgttgagacc gtgctccagt
     1861 gcgtcgaccc gccggaatgg ccggctggtg tccaagggtg gcggggtccc ttcggcgtcg
     1921 tcagcagaca tgtgcaccag cgtgacgcag tccggccaag cgcccctcga gttatccaca
     1981 cccgggccga cccagggcgg ctatcccctg gcttcgggtt ccgcccagaa agtcgcccca
     2041 ggggaggatt ccgccgctgg cgagctagta
//
LOCUS       DATAIX010000371         3851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_247147, whole genome shotgun sequence.
ACCESSION   DATAIX010000371 DATAIX010000000
VERSION     DATAIX010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247147"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VHM65_05565"
     CDS             complement(<1..569)
                     /locus_tag="VHM65_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX2705207.1"
                     /translation="MTSFEVRLPDVGEGVAEAELLSWSVDVGDQVTPASALADVMTDK
                     ATVEVACPVTGTVVALHGEPGDRLAVGSQLVTVELDDGAPMTSRPPTTATADVSGSAD
                     ASGPAERPERVATVEPPAEVDQPAAAESPARPDTDAATTPSGDRRRATAAPAVRARAR
                     ELGLDLAAVLGTGPQGRVTHADLDRHLLS"
     gene            complement(566..1579)
                     /locus_tag="VHM65_05570"
     CDS             complement(566..1579)
                     /locus_tag="VHM65_05570"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX2705208.1"
                     /translation="MPSMTMIEAIRDAHTVAMERDDRVVVFGEDVGLFGGVFRCTQGL
                     QQRFGPHRCFDTPISEAGIVGAAVGMAAYGLRPCVEIQFADYMYPAYDQIVSEAARLR
                     HRSAGDFTAPLVIRMPTGGGIYGGQTHSQSPEALFTHVAGLTTVVVSNPHDAKGLLLA
                     SIEDDDPVIFLEPKRLYNGPFDGHHDRPLVPWSQHPLGEVPEGHYTIPLGTARIHRPG
                     DELTVLTYGTLVHVAQAAAEETGIDAEIVDLRTLVPLDIDTVVASVTKTGRCLAVHEA
                     TLTSGFGGELSALVQERCFHHLEAPVTRVCGWDTPYPHAHEWSYFPGPERVGRAMREV
                     LAA"
     gene            complement(1581..2810)
                     /locus_tag="VHM65_05575"
     CDS             complement(1581..2810)
                     /locus_tag="VHM65_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX2705209.1"
                     /translation="MDPAPAGLRLRIPQAAPGDRPAGRVRFVSAAGEMPKPALNEKVE
                     RIALLRKQLVRILDDAGQAVGPWAPSDLTPEQKLSGLRDMLLQRAFDARMLRAHRQGM
                     ISFYMQALGEEAIACAQQRALRPGDMHFPTYRQHGLLIAAGYPLATMMNQILSNEYDP
                     LKGRQLPVLYSSKAHGFFSISGNLATQYIQAVGWAMASALRGGTEIASAWIGEGSSAE
                     SDFHAALVFASTYQAPVILNLVNNHWAISTPEDFARGASATFADRGYGFSIPALRVDG
                     NDYLAVYAASQWAAERARTNLGPTLIEWVTLRVGAHSTADDPSVYRSADEASRSPLGD
                     PIDRLKQHVIAIGAWDDERHAALVADLEAEVEETFAQAAALGSAKSGRVGDPRSMFDD
                     VYAELPRHLREQRAQVG"
     gene            2955..3104
                     /locus_tag="VHM65_05580"
     CDS             2955..3104
                     /locus_tag="VHM65_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705210.1"
                     /translation="MAHVVGQPRKELIVSEDRQDTRARLLTGDDDDVTAKPVRACQGD
                     VEVVV"
BASE COUNT          581 a         1280 c         1409 g          581 t
ORIGIN      
        1 ctgctgagca ggtgccggtc gaggtcggcg tgggtgaccc gcccctgggg cccggtgccg
       61 agcacagccg cgaggtcgag gccgagctca cgggcccgcg cccgcacggc gggtgcggcc
      121 gttgcgcgac gccgatcccc gctcggtgtc gtggcagcgt cggtgtctgg ccgagcaggt
      181 gactcggcgg ctgcgggttg gtcgacctct gcgggcggct cgacggtcgc gacacgctcg
      241 ggccgctcgg ccgggcctga ggcatcggct gagcccgaga catcggccgt cgcggtggtg
      301 ggcgggcggc tggtcatcgg agcgccgtcg tccagctcga cggtcaccaa ctggcttccg
      361 acggccagtc ggtccccggg ctcgccgtgc agggcgacga ccgtccctgt gacggggcag
      421 gcgacctcga cggtcgcctt gtcggtcatc acgtcggcca acgcggacgc cggggtgact
      481 tggtcgccga cgtcgaccga ccagctgagc agttcggcct cggcgacccc ctcgccgacg
      541 tccgggagcc ggacctcgaa gctggtcatg cggccagtac ctcgcgcatc gcccgcccga
      601 cacgttcggg ccccgggaag tacgaccatt cgtgagcgtg ggggtagggc gtatcccacc
      661 cgcagactcg ggtgacgggt gcctcgaggt gatggaagca acgctcctgc accagggcgc
      721 tgagctctcc gccgaacccc gacgtcaggg tggcttcgtg gacggccagg catcggccgg
      781 tcttggtcac cgacgcgacg acggtgtcga tgtcgagcgg gacgagcgtc cgcaggtcca
      841 cgatctcggc gtcgatgccg gtctcctccg cagccgcttg ggcgacgtgg acgagggttc
      901 cgtaggtgag gacggtcagc tcatctcccg gccggtggat ccgcgccgtg ccgaggggga
      961 tcgtgtagtg cccctcgggt acctcgccga gcgggtgctg cgaccagggc accagcggcc
     1021 ggtcgtggtg cccgtcgaac ggcccgttgt agaggcgttt gggctccagg aagatcaccg
     1081 ggtcgtcgtc ctcgatcgac gcaaggagga gccccttggc gtcgtggggg ttggatacga
     1141 cgaccgtggt caacccggcg acgtgggtga acagagcctc cgggctctgc gagtgggtct
     1201 gcccgccgta gatgccgccg ccggtgggca tccggatcac caagggggcg gtgaagtcgc
     1261 cggccgagcg atggcgcagc cgggcggcct cggacacgat ctggtcgtag gccgggtaca
     1321 tgtagtcggc gaactggatc tccacacaag gccgcaggcc gtaggcggcc atgccgaccg
     1381 ctgcaccgac gatgccggcc tcgctgatcg gtgtgtcgaa gcagcgatga ggtccgaacc
     1441 gctgttgaag gccctgggtg cagcggaaca ctcccccgaa gaggccgacg tcctcaccga
     1501 acacgaccac acggtcgtcg cgctccatcg cgacggtatg agcgtcgcgg atcgcctcga
     1561 tcatcgtcat gctcggcatg tcagcccacc tgcgcccgct gctcgcgcaa atgccggggg
     1621 agttcggcgt agacgtcgtc gaacatcgaa cgcggatcac cgacgcggcc gctcttggcc
     1681 gagccgaggg ccgcggcctg cgcgaaggtc tcctcaacct cggcctccag atcggccacc
     1741 agcgcggcgt ggcgctcatc gtcccaggcc ccgatcgcga tcacgtgctg cttgagccgg
     1801 tcgatcgggt cgccgagcgg tgaccgactg gcctcgtcgg ccgaccggta caccgatggg
     1861 tcgtcggcgg tggagtgggc accgacccgc agggtgaccc actcgatcag tgtgggcccg
     1921 agattggtcc gcgcccgctc ggcggcccac tgggaggcgg cgtacacggc gaggtagtcg
     1981 ttcccgtcca cccgcagggc ggggatgctg aacccgtagc cgcggtcggc gaaggtggcc
     2041 gacgcacccc gggcgaagtc ctcgggcgtg gagatcgccc aatggttgtt caccagattg
     2101 aggatcaccg gcgcctgata ggtggaggcg aagaccagcg cggcgtggaa gtcggactcg
     2161 gcggaggagc cctcgccgat ccacgccgag gcgatctcgg tgccgccccg cagcgccgac
     2221 gccatggccc agccgacggc ctggatgtac tgggtggcca ggtttccgga gatcgagaag
     2281 aatccgtgcg ccttcgagga gtacagcacc ggcagctggc ggcccttgag cgggtcgtac
     2341 tcgttggaga ggatctggtt catcatcgtc gccagcgggt accccgccgc gatcagcagg
     2401 ccgtgctggc ggtaggtcgg gaaatgcatg tcgcccggcc gcagggcacg ctgctgcgcg
     2461 caggcgatcg cctcttcgcc gagggcctgc atgtagaacg agatcatgcc ctgccggtgg
     2521 gcacgcagca tccgcgcgtc gaaggcccgc tgcaacagca tgtcgcgcag cccggagagc
     2581 ttctgctccg gcgtcaggtc ggatggagcc cacggcccaa cggcctggcc cgcgtcgtcc
     2641 aggatccgga cgagttgctt gcgcaagagg gcgatgcgct cgaccttctc gttgagggca
     2701 ggcttgggca tctcgcccgc cgcgctgacg aaccggacgc ggccagccgg ccggtcgcca
     2761 ggggcggctt gggggatgcg aaggcggagc cctgcgggcg ctggatccat gcgacgactg
     2821 tgctgggggg caaacggcgg cgtcaagtac gccgtgcagg cccacagcgc ccgagcgcga
     2881 ggtggtctgc ggactcaggc tccacccaag ccgtcggtga gggcctgaag cctgcgcggg
     2941 tcgcggctgc ctgcgtggcc catgtcgttg gacagccgag gaaggagctc atcgtctccg
     3001 aggatcgaca ggacacccgc gcgcgcttgc tcaccggcga cgacgacgat gtcacggcca
     3061 agccggtgcg cgcctgccag ggtgacgtcg aagtcgtagt gtgatcgccc ctgtggagca
     3121 cgcgcccagc acgtgtccat ggccgcaggc ggtgggacgc tgccgctact ggagagtgcc
     3181 gtctactgtc gatggcggcc gttcggaagc ggcgatcccc gcgctacttc atcgacgtgc
     3241 atgagcaggc agtcctgtgt gcgcaccaag tagcgcaatc ccagctggta ccagcgcgga
     3301 aagaccggcg gtagctcgct gaggcgcatc aatcgaggcc cggtgagccg caggacttct
     3361 cccctgagct gcatctcgca ccctcccgcg gaccggatgt tcctcaccca ttcggaggcg
     3421 gcgccaaagt tcgctcctac ggcaacgagg tcatggcggc ggaacgctcg gacaggggtt
     3481 cggtacgtgc gtccgcttcg acgacccgtg tggatgacta cggcaagtga acccactcgt
     3541 ccagcaaggc ggagagcgac gggattgatg actgtgtgag ccggcctcag gaacgttggc
     3601 cagggcatcg agcctcacct ccaacccttc acgatactcc cggggcaggg aggatgatct
     3661 tccgctcatg ccgcgactag tgtggccggg caccggccgc gcagacgcgg cgaagccacc
     3721 gtccgcaatt gccgcgtagc gtggcctccg gccgcggtgg acgcggcgcc atccccgcac
     3781 ggacatgccg cgagccgcgc ggtctccgag atgacgggtc gtgacgctgg ggagtgttcg
     3841 gaggcggtcg g
//
LOCUS       DATAIX010000372         3512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_80277, whole genome shotgun sequence.
ACCESSION   DATAIX010000372 DATAIX010000000
VERSION     DATAIX010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80277"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VHM65_05585"
     CDS             complement(<1..893)
                     /locus_tag="VHM65_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2705211.1"
                     /translation="MTISLIWGSSFLLIKLALDAFTPLQVATGRILTGTAAVAVMAWA
                     TGARPPRERRVWAHLMVSAFFLCTLPFLLFPLGEERISSALAGIGNATTPLAAVVFTG
                     LMIPADRLSARKLVAVGVGFLGVVVIMSPWQTQGRPDLLGFSMTVVAGASYGLGWTYV
                     KRFLSSSDLGGLSLPAAQLSVASVQVSLALVLTWLVAPGGRGAPWSPAVPAGHSLRPA
                     LLALLMLGVVGTGVAYNLQFDLVRAVGPQIATTITYLIPIVAVLLGIAFLDERLTSWQ
                     LAGAGIVLVAAVVIGLPGRRR"
     gene            complement(941..1171)
                     /locus_tag="VHM65_05590"
     CDS             complement(941..1171)
                     /locus_tag="VHM65_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705212.1"
                     /translation="MSMLLEEATWTDRAGIHFTQPEIVLLFKARHQRAKDEANVAATL
                     PLLSACRRDWLRTALAQVHPDHDWVHRLGAEA"
     gene            complement(1216..1575)
                     /locus_tag="VHM65_05595"
     CDS             complement(1216..1575)
                     /locus_tag="VHM65_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705213.1"
                     /translation="MPQLPQEIFESLYGAWAPRSPEDAAILFAGLGVPWWAVGGRALE
                     AFTGVSRHHDDIDLDAMWSALPALRDHFDGRRHVWAAWQGGIHPLLPDAPDELPDGCG
                     QVGPRENAASHLSSTSS"
     gene            complement(1601..2560)
                     /locus_tag="VHM65_05600"
     CDS             complement(1601..2560)
                     /locus_tag="VHM65_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX2705214.1"
                     /translation="MAEVLVLVDHVDGTVRKTTTEMLTIARRLGEPAAVFLGAGYDAA
                     KSTLAQYGAVKVYRAESGDLADYLVAPQAELLATLVERTSPAAVLIASSSEGKEVAAR
                     LAIKTSSGLITDAVDVQAGQGGAVQTIQSVFAGSYTVTATVTHGTPIIAVKPNSAQPE
                     PADADAVEEVLDVVISAAAKGARIVEANPRAATGRPELTEATIVVSGGRGTAGDFSKV
                     EAFADSLGAAVGASRAAVDSGWYPHTSQVGQTGKQVSPQLYVACGISGAIQHRAGMQT
                     SKTIIAINKDEEAPMFELVDFGVVGDLFAVLPQATEAVNAAKG"
     gene            complement(2592..3383)
                     /locus_tag="VHM65_05605"
     CDS             complement(2592..3383)
                     /locus_tag="VHM65_05605"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX2705215.1"
                     /translation="MNIVVCVKHVPDAQGARTFDASDNTTDRANVDGLLSELDEYAVE
                     EALKIVEESEGEVIVLTVGPDQAADAIKKALQMGADRGVHVNDAAIHGSDAAATSLVL
                     AEAIKKVRADSTVDLVLTGMASTDGTMGVVPSMLAERLGLPQVTFASELTVTGSGVSI
                     RRDGDAASQTIEASLPALVSVTDQINEPRYPSFKGIMAAKKKPVESWTLADLGLDASQ
                     VGLGAAWTKVESFTARPPRQQGQIVKDDGDGGAKLAAYLVEHKFV"
BASE COUNT          550 a         1164 c         1288 g          510 t
ORIGIN      
        1 attctacggc ggcccgggag accgatgacg acggccgcca cgagcacgat gcccgccccg
       61 gccagctgcc acgaggtgag ccgctcgtcg aggaaggcga tcccgaggag cactgcgacg
      121 atggggatga ggtaggtgat cgttgtcgcg atctggggac cgacggcgcg gacgagatcg
      181 aactggagat tgtaggcgac acccgtgccg acgaccccga gcatgagcag ggccagcagg
      241 gcgggccgca gggagtgtcc tgcggggacc gctggcgacc acggcgcccc ccgcccgccc
      301 ggcgcgacga gccaggtgag gaccagggcc agactcacct ggacgctcgc gaccgacagc
      361 tgcgccgcgg gcagtgacag gccgccgagg tccgaggagc tcaggaatcg cttgacgtag
      421 gtccagccca gcccgtacga cgcgccggcg acgaccgtca tcgagaagcc gagcaggtcg
      481 ggacgcccct gggtctgcca gggcgacatg atgacgacga cgccgaggaa gccgacgccc
      541 accgcgacga gcttgcgtgc cgagagccgg tcggccggga tcatcagccc ggtgaagacg
      601 accgcggcga gcggcgtcgt cgcgttgccg atcccggcca gggccgagga gatgcgctcc
      661 tcgccgaggg ggaacaggag gaacggcagc gtgcacagga agaacgccga caccatgaga
      721 tgcgcccaga cccggcgctc gcgcggcggt cgcgctccgg tggcccaggc catgacggca
      781 accgcggcgg tcccggtgag gatgcgaccc gtggcgacct gcagcggcgt gaaggcatcg
      841 agagcgagct tgatgagcag gaagctcgaa ccccagatga gcgagatcgt cacgaacttg
      901 acctgccatg gcacccgctg ggcccgtggg ctggtcgcca tcacgcctct gcgcccaggc
      961 ggtgcaccca gtcatggtcc gggtgcacct gcgcaagggc tgtgcgcagc cagtcccggc
     1021 gacaggccga caacagcggc agggtcgcgg cgacgttggc ctcatccttg gcgcgttggt
     1081 gcctggcttt gaagagcagc acgatctcgg gctgggtgaa gtgaatgccc gcccggtcag
     1141 tccaggtggc ctcctcgagc agcatggaca tcggcgggac ccggcggttg acccattctc
     1201 cgcccttccc ggggttcatg aggatgtcga gctcagatgg gacgcagcgt tctcgcgggg
     1261 cccaacttgg ccgcacccgt ccggcagctc gtccggggcg tcgggaagca gtgggtggat
     1321 tcccccctgc caggcggccc agacatggcg ccgtccgtcg aagtggtcgc gcagcgcggg
     1381 cagggccgac cacatggcgt cgaggtcgat gtcgtcatgg tgacggctga ccccggtgaa
     1441 ggcctcaagg gctcgcccgc ccaccgccca ccacgggaca ccaagaccgg cgaagagtat
     1501 ggcggcatcc tcgggcgatc gcggcgccca cgcgccatac agggactcga agatctcctg
     1561 gggcagctgt ggcacccggt cagactaacg agtcaggcgg tcagcccttg gcggcgttca
     1621 cggcctcggt ggcctgcggc aacacggcga agaggtctcc gacgacaccg aagtcgacga
     1681 gctcgaacat cggggcctcc tcgtccttgt tgatggcgat gatcgtcttg gatgtctgca
     1741 tcccggctcg gtgctggatc gcgccggaga tgccacaggc gacgtagagc tgcggcgaga
     1801 cctgcttgcc cgtctgaccg acctgggagg tgtgcgggta ccagccggag tcgaccgcgg
     1861 cgcgagaggc cccgaccgcg gcaccgagcg agtcggcgaa cgcctcgacc ttggagaagt
     1921 cgccggccgt gccacgccca ccggagacga cgatcgtggc ctcggtcagc tcggggcgcc
     1981 cggtcgcggc cctggggttg gcctcgacga ttctggcgcc cttggcggca gcggagatga
     2041 ccacgtcgag cacctcctcg acggcgtccg cgtcggcggg ctcgggctgg gcggagttgg
     2101 gcttgacggc gatgatcggg gtcccgtggg tcaccgtggc cgtgacggtg taggagccgg
     2161 cgaagaccga ctggatggtc tggactgccc cgccctggcc tgcctggacg tcgacggcgt
     2221 cggtgatcag gcccgacgag gtcttgatcg cgagtcgcgc ggcaacctcc ttgccctcgg
     2281 acgaggaggc gatcagcacg gccgccgggg aggtgcgctc gacgagagtg gcgagcagct
     2341 cggcctgggg cgcgacgagg tagtcggcca ggtcgccgga ctcggcgcgg tagaccttca
     2401 cggcgccata ctgcgcgagc gtcgacttgg cggcgtcata cccggcgccg aggaagactg
     2461 ccgccggctc gccgagccgg cgggcgatcg tcagcatctc ggtggtcgtc ttgcgcacgg
     2521 tgccatcgac gtggtcgacg aggacgagaa cttcagccat gtgggtcatc tctcctggcg
     2581 gtggcggtca gtcagacgaa cttgtgctcg acgaggtagg cggcgagctt ggctccgccg
     2641 tcgccgtcgt ccttgacgat ctgcccctgc tggcggggtg ggcgcgccgt gaacgactcg
     2701 accttggtcc aggcggcacc gaggccgacc tgggaggcgt ccaggccgag gtcggccagg
     2761 gtccaggact cgaccggctt cttcttggcg gccatgatcc ccttgaagga cgggtagcgc
     2821 ggctcgttga tctggtcggt gaccgagacg agcgccggca gcgaggcctc gatcgtctgg
     2881 gacgcagcgt caccgtcgcg ccggatcgag acgccggacc cggtcacggt gagctcggag
     2941 gcgaacgtca cctgtggcag cccgagacgc tcggcgagca tcgaggggac cacccccatc
     3001 gtcccgtcgg tcgaggccat gccggtcagg acgaggtcga ccgtcgagtc ggcccggacc
     3061 ttcttgatcg cctcggcgag gacgagcgag gtcgcggccg cgtcggagcc gtggatggcc
     3121 gcgtcgttga cgtgcacccc gcggtcggca cccatctgca gggccttctt gatggcatcg
     3181 gcggcctggt ccgggccgac ggtgaggacg atgacctcgc cctcggactc ctcgacgatc
     3241 ttgagtgcct cctcgacggc atactcgtcc agctcggaca gcagcccgtc gacgttggcc
     3301 cggtcggtcg tgttgtccga ggcgtcgaag gtccgcgcac cctgcgcgtc ggggacatgc
     3361 ttgacgcaga cgacgatgtt catggtgatg gctgttcctc ttcccggtgg aggggttgac
     3421 agatccgctc ccatcctgac atccccggct cggggggacc cacccactca ggggtgatcc
     3481 ccgacacgac cggatgcggc cctccccctc gt
//
LOCUS       DATAIX010000373         4879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_50257, whole genome shotgun sequence.
ACCESSION   DATAIX010000373 DATAIX010000000
VERSION     DATAIX010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50257"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..563
                     /locus_tag="VHM65_05610"
     CDS             183..563
                     /locus_tag="VHM65_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705216.1"
                     /translation="MSRNGRIVLGLLLFNAVSAVGGGVALMTGLIPEQVSWIRETDFS
                     SLYFPGVILMAIVGGSSLVAAFAMMKRAAGWQLASILAGVIMLFWIVGEIASIRGFHF
                     LQVIYFITGAALIWCTPQGSLVSE"
     gene            complement(600..929)
                     /locus_tag="VHM65_05615"
     CDS             complement(600..929)
                     /locus_tag="VHM65_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705217.1"
                     /translation="MFARPRRIRHHTAWHRHGTDTAGHRWDGLTRGGPLHLNDEQVCR
                     ALAESAHLTGTVALARVRARCLDAAVYDGADVRIHADRMPAWNQPPRVTVLLEADQEP
                     AMDAASR"
     gene            1261..1572
                     /locus_tag="VHM65_05620"
                     /pseudo
     CDS             1261..1572
                     /locus_tag="VHM65_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
     gene            1657..2664
                     /locus_tag="VHM65_05625"
     CDS             1657..2664
                     /locus_tag="VHM65_05625"
                     /inference="COORDINATES: protein motif:HMM:NF016488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705218.1"
                     /translation="MGSPDIDRAVSEFAASQPRLVAASEAFVALVTGILDDAGINYLS
                     VTGRAKTVASFAAKATRREGDQLVHRDPLRGITDQVGVRVITYVQSDVLAVAELLADQ
                     LSVLEDRDLGRETARAGRFGYASRHLLVSLDEGEHPAYHPTRCASVQLRTVLQHAWAE
                     FEHDIRYKGTVPPEHVPDLDRRFTLAAGLLELADREFSTIRDVLQAGMGGLAPTADEG
                     DPRISAQELATFLAGRYASAGWSRTEHYAWICGLLLELGIASLDELSDVLRAIDTTAI
                     TGHMGYRYPAGAVRRLDDDLLARFGTRYVGLRGNAHRRDGLLARLGRLESREPGPARL
                     A"
     gene            2747..3586
                     /locus_tag="VHM65_05630"
     CDS             2747..3586
                     /locus_tag="VHM65_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2705219.1"
                     /translation="MHRSWVTSGQAELAVVDWGGDGTPIVALHPNIADSRVWRECAPA
                     WVAAGYRVVSYDRRGFGQTRYAEEAHDAIEDLRAVTAATSCRPAVIVGNSRGGALALE
                     LAVAHPRDVLALVLIGAAPTGYPDEEWVAGQAESEQDALLEAAETAGDLDLVNRLEIR
                     YWLDGTEQPEGRVGGPARDLMLEMNSDALRAEPIGEQREREPVWPRLAEVTVPVLVIA
                     GGLDLPGLAGLSAQLTEALPDARLVTMAGSAHCPSMDRPEQLSRVVIDFLTATGHIRN
                     LTG"
     gene            3697..4554
                     /locus_tag="VHM65_05635"
     CDS             3697..4554
                     /locus_tag="VHM65_05635"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2705220.1"
                     /translation="MSESLSRVFSALSDPTRRQILQRLGEQSATVGELARPFPISLPA
                     ISRHLKVLEAAGLITRDRDAQWRRASLRGESIREAMTWLGQLNQVWGAATRPPRRPPR
                     RDEAPPAGVARDPGNSYRQGTELMNDPTYEVCISRYFDAPPEMVYRAFVDPAQLAQWF
                     GPLVFHVPASTVDIDARPGGHWRMTMVNNDDPQWQSPISSTFVEVVENRLLVGYELAQ
                     GFPGIEDGTKLSLSLEFLPDGDGTRLELRQGPFPEQLRTMSETGWGQSFFKLDGLLAT
                     PEEFRPSTT"
     gene            complement(4618..>4879)
                     /locus_tag="VHM65_05640"
     CDS             complement(4618..>4879)
                     /locus_tag="VHM65_05640"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX2705221.1"
                     /translation="VEDFSAGMVRFIGVVELLAAIGLILPAATGIAPVLTPLAATGLA
                     VIMVLAAVTHLRRKEASALPINAGLFVLAVLVAWGRFGPYSF"
BASE COUNT          725 a         1720 c         1712 g          722 t
ORIGIN      
        1 cctcgcacgt ggtctggtcg gccactcgca cgtgaccgtg atgggcgctg acgcacgggc
       61 ttgtggggcg caagcagcgc ctggtgcacg cagagaaggc gcaccgctat cacgaccatg
      121 aacgcgccct gcggttcgcc ggtcccctat gggagcgact ggcgttggta tagagtgtcg
      181 acatgagtag aaatggcagg attgtcctcg ggttgctgct gttcaatgca gtctcggcag
      241 tgggcggggg tgttgctctc atgactggcc tgatcccgga gcaagtgtca tggatccggg
      301 agacggactt cagctctctc tatttccccg gcgtgattct tatggccatt gtcggtggca
      361 gctctttggt cgccgctttc gcgatgatga agcgcgctgc tggatggcag ctggccagca
      421 tcttggctgg ggtgatcatg ctgttttgga tagtcggaga gatcgcctca attcgcggat
      481 tccatttcct gcaagtcatc tatttcatta ccggcgcggc gctcatctgg tgcacgcctc
      541 aggggagcct ggtctccgag tagcgcacct ggtcggtgac ggagccggta gcgcggacct
      601 caccggctcg ccgcgtccat ggcaggctcc tgatctgcct cgaggaggac cgtcactcga
      661 gggggctggt tccacgcggg catgcggtct gcgtggatcc gcacgtcggc accgtcatag
      721 accgctgcgt cgagacagcg cgcccgcacc cgggcgagcg cgacggtgcc ggtgaggtgc
      781 gcactctcgg cgagtgcccg gcatacctgc tcgtcgttga ggtggagggg gccgccgcgg
      841 gtgagcccgt cccagcggtg gccggcggtg tcggtgccgt gccgatgcca tgcagtgtgg
      901 tgacgaatcc ggcgaggtcg cgcgaacacg tcggcgtcgc cgatggcctg gcggctcgta
      961 gccccgccgg gccaccggct ccgaccagcg ggcgtgggcc ggtggcccga cccggctggg
     1021 acgaggggtc ccggccgagg gcacccaagc ccgctgaccc gtcatacact gccgggcagc
     1081 tcgccgtccc caagcatctc gtcgacgtcc acgacgcgct ccgtgccgag ctcacccagg
     1141 tgcgctcggt cgtcgaccag gtgcgccagg gcttccttgc cgtgggcccg gcgcggtcgc
     1201 tgatcaccac gatggcactg cggccgaaca gctggacgct cgggggcctt ctgcgagtcc
     1261 tactgccgca tcgtcaccgg ccaccacacg ctggaggacc acagagtcct cccccacctg
     1321 cgccgggccg atcccgccct cgcccccgtg ctcgaccggc tcgaagccga gcatcacgtc
     1381 attgccggcg tgctcgacca gctcgaccgc gccctcgtca cgctcgtggc ggccgaggac
     1441 cagcgggccg ggcaggccgc gctggacgac ctcggggccc gggtcgacct gctcaccgac
     1501 acgctgctgt cccacctgtc ctacgaggag ggggagctct tcgacccgct ggcccgccac
     1561 gggttcggct gagcccgggg cctgccgtgg ccggcgtcgg tcacggcccg agctcacccg
     1621 ctggcgccgc gggcccgggc cgggcagggt gcacccatgg gatcacccga catcgaccga
     1681 gcggtcagcg agttcgcggc gagccagccg cgcctggtgg ccgcgagcga ggcgttcgtc
     1741 gccctcgtga ccggcatcct cgacgacgcg gggatcaact acctcagcgt caccggccgg
     1801 gccaagaccg tcgcgtcctt cgcggccaag gccacccggc gcgaagggga ccagctggtt
     1861 catcgcgacc cgctgcgcgg gatcaccgac caggtggggg ttcgggtgat cacctacgtg
     1921 cagagcgacg tgctggccgt ggccgagctc ctggcggacc agctgagcgt gctcgaggac
     1981 cgggacctgg ggcgggagac cgcgcgggcc ggacggttcg gctacgccag ccggcacctg
     2041 ctcgtgtccc tggacgaggg tgagcacccg gcataccacc ccacccggtg cgcctcggtg
     2101 cagctgcgca cggtgctgca gcacgcgtgg gccgagttcg agcacgacat ccgctacaag
     2161 ggcaccgtgc cgccggagca cgtgcccgac ctagaccggc ggttcacctt ggcggccggt
     2221 ctgctcgagc tcgccgaccg cgagttctcc acgatccgcg acgtgctcca ggccgggatg
     2281 ggcggcctcg cgccgacagc cgacgagggc gatccccgga tcagcgccca ggagctggcg
     2341 accttcctcg cagggcgcta cgccagcgcc gggtggtcgc gcaccgagca ctacgcctgg
     2401 atctgcgggc tcctgctcga actcggcatc gcctcgctgg acgagctgag cgacgtcctg
     2461 cgggccatcg acaccaccgc gatcaccggg cacatggggt accgctaccc tgcgggagcc
     2521 gtccggcgcc tcgacgacga cctgctcgcc cggttcggca ccaggtatgt cgggctgcgc
     2581 ggcaacgccc accggcgaga cggtctcctg gcgcggctcg gacggctcga gtcccgtgag
     2641 ccagggcctg cgcggctcgc ctgagcagaa gtgggcacgc catcgtcgcc cccgtccgga
     2701 gaaacccgct gtcgtcgggg tctccccgtc ggcgagactg ggccgcatgc atcgctcatg
     2761 ggtcacctct ggtcaggccg agctcgccgt cgtcgactgg ggcggtgacg gcacgccgat
     2821 cgtggccctt cacccgaaca tcgcggacag ccgggtatgg cgtgagtgcg ctcccgcgtg
     2881 ggtggccgcc ggctatcggg tcgtctccta cgaccgccgg gggttcggtc agacgcggta
     2941 tgccgaggag gcgcacgacg ccatcgagga cctgcgggcc gtgaccgcgg cgacctcgtg
     3001 ccgcccggcg gtcatcgtcg gcaacagccg aggcggggcc ctggcgctcg agctggcggt
     3061 ggcgcatccc cgtgacgttc tcgcgctggt gctcatcggc gccgcaccga cgggctaccc
     3121 cgacgaggag tgggtggccg gccaggccga gtcggagcag gacgcgctgc tcgaggcagc
     3181 cgagaccgcg ggcgacctcg acctcgtcaa ccgcctcgag atccgctact ggctcgatgg
     3241 gaccgagcag ccggagggcc gcgtgggcgg tccggcgcgc gacctcatgc tggagatgaa
     3301 cagcgacgcg ctgcgggccg agccgatcgg ggagcagcgt gagcgggagc cggtctggcc
     3361 acggctcgcc gaggtcaccg tcccggtcct cgtcatcgcc ggcgggctcg acctgccagg
     3421 cctcgcgggc ctgagcgcgc agctgaccga ggcgctgccg gatgcgcggc tggtgacgat
     3481 ggccggctcc gcacactgcc cgtcgatgga tcggcccgaa cagctcagcc gggtcgtcat
     3541 cgacttcctc accgccacag ggcacatccg gaacctcacc ggctgaggac accctggaac
     3601 ccatggtgcc caggtcaccc gcgcgcacgg ggggttgacc cgggggaggg gccgacatac
     3661 gcttaaccaa ctcattaatt agcgagatgg ttaagcgtgt cggagtcact gagccgggtg
     3721 ttctcggccc tgtccgaccc cacccgacgg cagatcctgc agcgcctggg cgagcagtcg
     3781 gcgaccgtcg gcgagctggc gaggccgttc ccgatcagcc tgccggccat ctcccgtcac
     3841 ctcaaggtgc tcgaagccgc cggcctgatc acccgcgaca gggacgccca gtggcgtcgc
     3901 gcctcgctcc gcggggagtc gatccgcgag gccatgacct ggctggggca gctgaaccag
     3961 gtctggggag cagcgactcg accgcctcga cgcccacctc gacgcgatga agcgccaccg
     4021 gccggggtcg ctcgcgaccc cgggaactcc taccgacaag gaaccgagct catgaacgac
     4081 ccgacctacg aggtctgcat ctcccgctac ttcgacgctc ctccggagat ggtctaccga
     4141 gcgttcgtgg accccgctca gctcgcgcaa tggttcggac ccctcgtctt ccacgtcccg
     4201 gcgagcacgg tcgacatcga cgcccggccc ggagggcact ggcggatgac gatggtcaac
     4261 aacgacgacc cgcagtggca gtctccgatc agctcgacct tcgtcgaggt cgtcgagaac
     4321 cggctgctcg tgggctacga gctcgcccag ggcttccccg ggatcgagga cggcaccaag
     4381 ctcagcctga gcctcgagtt cctccccgac ggcgacggca cccggctcga gctgcgtcag
     4441 ggaccgttcc ccgagcagct tcgcacgatg agcgaaacag gctggggtca gtcgttcttc
     4501 aagctcgacg ggctgctcgc cacccccgag gagttccgcc cgtcgacgac gtgacgcagc
     4561 accggccaga tccctgaccg atgtcggaca aacagcagcc tcgcgtggcc gtgcggccta
     4621 gaagctgtag gggccgaagc ggccccaggc gacgagaacg gcgaggacga acaggccggc
     4681 gttgataggg agggcgctgg cctccttgcg gcgcaagtgc gtgacggccg cgaggaccat
     4741 gatgacagcg aggcccgtgg cagccagcgg ggtgagcacc ggggcaatcc cggtcgcggc
     4801 gggaaggatc aagccgatcg cggccagcag ctcgaccacg ccgatgaacc tcaccatgcc
     4861 ggcagagaag tcctcgacc
//
LOCUS       DATAIX010000374         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_109890, whole genome shotgun sequence.
ACCESSION   DATAIX010000374 DATAIX010000000
VERSION     DATAIX010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109890"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VHM65_05645"
     CDS             <1..1003
                     /locus_tag="VHM65_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2705222.1"
                     /translation="PKGAELTHANLHLNASRSATVIMEMTHDDVIMGCLPLFHVFGLT
                     CGLNAATVVGATLTLIPRFDPAKALEVVGRDKVTIFQGVPTMYAAMLNHPDADAAETS
                     SLRVCASGGSAMPVEIMRAFEAKFSCMILEGYGLSETSPVASFNMPDRERKPGTIGVA
                     IPGCEMRLVDIGGHEAAAGEVGEIAIRGDNVMKGYWNKPEATLEAIPDGWFRTGDLAT
                     VDDEGYFTIVDRKKDMIIRGGLNVYPREVEEVMYQHPDVLEVAVVAIPDPVMGEEIGA
                     AVALRPGATSTLDDVQAFVKDRIAAYKYPRVLWSMAELPKGPTGKILRREVHPPTAG"
     gene            1160..2395
                     /locus_tag="VHM65_05650"
     CDS             1160..2395
                     /locus_tag="VHM65_05650"
                     /inference="COORDINATES: protein motif:HMM:NF037017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX2705223.1"
                     /translation="MTSGTTRHRALRGLALFAAAGLLASACSSTGVALTSSSPTTPAE
                     SSSTTVGPAPEVALAASVTDGATVGVDTAVSVTATAGTLSKVALASKGTFKENQGAVP
                     GTLSQDRSTWTAGRFLDPARTYTLTMTGANADGIAKTSTLTFKTKTLGIGQQAVVYPV
                     PGNGSTVGVAMPVIMQFDLPVKDKAAFEKRMVVTSVPAQRGSWRWISSKEVHWRPAGY
                     WKPGTKVSLKAALNGVPAGGGIYGMSDRSATFTVGRLFVMKADLKKTHSLRVYAKGKL
                     VRTIPVSGGRSSNQSRSGNKVIMSKAYHVVMDAATLNIPKNDPDYYRLDVYYAMRETW
                     SGEFLHAAPWSVGSQGRTNVSHGCIGMSTSNARWLYENVLVGDPVNVTGTSRHLEEGN
                     GWTDWNTSYEQFKKGSALA"
BASE COUNT          422 a          881 c          772 g          339 t
ORIGIN      
        1 gcccaagggc gccgagctca ctcacgccaa cctccacctc aacgcctcgc gctcggcgac
       61 cgtgatcatg gagatgaccc acgacgacgt catcatgggc tgcctgccgc tcttccacgt
      121 cttcggcctc acctgcgggc tcaacgcggc gacggtggtc ggcgccacgc tgaccctcat
      181 cccgcgcttc gacccggcca aggccctcga ggtcgtcggg cgcgacaagg tgacgatctt
      241 ccagggcgtg ccgacgatgt atgccgccat gctgaaccac ccggacgccg acgcggccga
      301 gacctcaagc ctgcgggtct gcgcgtccgg cggctcggcc atgcctgtcg agatcatgcg
      361 ggccttcgag gccaagttca gctgcatgat cctcgagggc tacggcctgt ccgagacctc
      421 ccccgtcgcc tccttcaaca tgcccgaccg cgagcgcaag cccgggacga tcggcgtcgc
      481 gatcccgggc tgtgagatgc gcctcgtcga catcgggggc cacgaggccg ccgccggcga
      541 ggtcggcgag atcgcgatcc ggggcgacaa cgtcatgaag ggctactgga acaagcccga
      601 agccacgctc gaggccatcc ccgacggctg gttccgcacc ggcgacctcg ccacggtgga
      661 cgacgagggc tacttcacga tcgtcgaccg caagaaggac atgatcatcc gcggcggcct
      721 caacgtgtac ccccgcgagg tcgaggaggt catgtaccag caccccgacg tcctcgaggt
      781 tgccgtggtc gcgatcccgg accccgtcat gggcgaggag atcggcgcgg cggtcgcgct
      841 tcgcccgggg gcgacgagca cgctcgatga cgtccaggcc ttcgtcaagg accggattgc
      901 cgcctacaag tacccacggg tgctctggtc gatggccgag ctgcccaagg gtccgacggg
      961 caagatcctg cgccgcgagg tgcacccccc cacggccggc tgagcccgca cggcagctcg
     1021 ggtccgcctc ccgtgagcta agatccacct atctgtgctt actcatgagg gcgtccgtcc
     1081 ttgaataagc aacaatggtc ctaacgcgta tggcggcaca acggcccgtg ccacagcgtg
     1141 acacaccctt ggagcgccca tgacctcagg cacgacgcga caccgtgccc tccgcggact
     1201 ggccctgttc gccgccgccg gactgctcgc ctccgcgtgc agctcgaccg gagtggccct
     1261 cacctcgtcc tcgcccacga caccggccga gagctcgtcc accacggtcg ggccggcccc
     1321 tgaggtcgcc ctggccgcct cggtgaccga cggcgccacg gtcggggtcg acacggcagt
     1381 ctcggtcacc gccacggcgg gcacgctgag caaggtcgct ctcgcctcga agggcacctt
     1441 caaggagaac cagggcgcgg ttcccggaac cctctcgcag gacaggtcga cctggacggc
     1501 cgggcgcttc ctcgaccccg cccgcaccta cacgctgacg atgaccggtg cgaacgccga
     1561 cggcatcgcg aagacctcca ccctgacctt caagaccaag acgctcggga tcggccagca
     1621 ggccgtggtg taccccgttc ccggcaacgg gtccaccgtc ggcgtggcca tgcccgtcat
     1681 catgcagttc gacctgccgg tcaaggacaa ggccgccttc gagaagcgca tggtcgtcac
     1741 ctcggttccg gcgcagaggg ggtcgtggcg ctggatcagc agcaaggagg tccactggcg
     1801 cccggcgggc tactggaagc cgggcaccaa ggtcagcctc aaggccgcac tcaacggcgt
     1861 ccccgccggg ggcggaatct acggcatgag cgaccggagc gccacgttca ccgtcgggcg
     1921 gctgttcgtc atgaaggccg acctcaagaa gacccactcg ctcagggtct acgccaaggg
     1981 caagctcgtc cgcaccatcc cggtgtcggg cggcagatcg agcaaccaga gccgcagcgg
     2041 caacaaggtg atcatgtcca aggcctacca cgtcgtcatg gacgcggcga cgctcaatat
     2101 ccccaagaac gacccggact actaccggct cgacgtctac tacgccatgc gcgagacgtg
     2161 gtcgggcgag ttcctgcacg ccgcgccgtg gtcggtcggc agccaaggcc ggaccaacgt
     2221 gagccacggc tgcatcggga tgagcacgag caacgcccgc tggctctacg agaacgtcct
     2281 cgtcggcgac ccggtcaacg tcaccgggac gtcccgccac ctcgaagagg gcaacggctg
     2341 gaccgactgg aacacctcct acgagcagtt caagaagggg tcggccctgg cttagccggc
     2401 tcgaccggcc cggg
//
LOCUS       DATAIX010000375         3143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_247260, whole genome shotgun sequence.
ACCESSION   DATAIX010000375 DATAIX010000000
VERSION     DATAIX010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247260"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1696)
                     /locus_tag="VHM65_05655"
     CDS             complement(<1..1696)
                     /locus_tag="VHM65_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705224.1"
                     /translation="MRILDGPEPLSIAAERARIRVLLALSSAEFERSGRRAALPVLRA
                     AVEAARAVGDDELTALAHSQRAMVLARSGDLGAALDALDRAADSVDALGPRDQCVLLN
                     NRGMLMLHRGEISTARAEFGRSRQIAARHGLNRQEFMAVHNLGHTAYLAGDLAAALEL
                     MAEADRIDTDVSRAAAWHDQGRVLLDAGLISEAAQRLREAAALGAERRQQHVHSEIEL
                     DLARALALLGEPSSAAALARRAQAVFARRRAPVWAARAQLVALGIELPRSRRPRSIAL
                     TARRVAERAGLAGDPALVFDARLLEADAWLAAGAVVDAEGALAQVQSSTSLTPRLHRS
                     LVEATLHASRGEQTRAATVLRTAATDLRRAQSRTASLDLRTATAVHGVQLAVQDMHLA
                     LARSPSALLASTERWRSATGRLPSVRAADPAVAELMASLRDVQEQIRRGAPSLDVLDR
                     AAQLQREIREHGWTAAAQPASGRERQPLGVSRLREALLEDGSDLVSFAVVGDTLVGVG
                     IVGGRVVQPTVASAERVRQTCLRAATDLAAAASFHLGPLWGPVWGSLEATLAELDRV"
     gene            complement(1896..2360)
                     /locus_tag="VHM65_05660"
     CDS             complement(1896..2360)
                     /locus_tag="VHM65_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705225.1"
                     /translation="MPERLDDVDFLILATLRETQSRLDPVPVDLVERIKFALTVRALD
                     AEIAELTTMALLPTRDDTAMADTVTFTAGSLSLMVTITAGAGETSRIDGWVTAPRAKI
                     EVSVDDWATELVADAHGRISVEGVPHGRVQFVIWPDPASPAATPVVTPGITI"
     gene            complement(2364..2957)
                     /locus_tag="VHM65_05665"
     CDS             complement(2364..2957)
                     /locus_tag="VHM65_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2705226.1"
                     /translation="MDQAVTPSGPTRETLGGRAALAFDAYRAGDRQRMGDLVDLLTPV
                     LWRTARAQGASVALAEDAVQLAWTQLVDLTDTIKDPAGVPSWMVVTVKREAWRLMRQD
                     RRTIPVDVVPEEVSSDPGPEALSILTEHQRILWGHVRALPDRCRELLEVIAFVDRPDY
                     ASLAQSLGMPVGSIGPTRGRCLTKLRSALAADSRWSV"
     gene            complement(2942..>3143)
                     /locus_tag="VHM65_05670"
     CDS             complement(2942..>3143)
                     /locus_tag="VHM65_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705227.1"
                     /translation="PRATVDPDDLSGGFGIWSGTSFAAPVLAGELAAHLAADPDIGKP
                     DLASALERGWRALEEELPWTRP"
BASE COUNT          434 a         1108 c         1198 g          403 t
ORIGIN      
        1 ggacccggtc gagctcggcc agggtcgcct cgagcgagcc ccagaccggc ccccagaggg
       61 ggccgaggtg aaacgatgcg gccgccgcca ggtccgtcgc ggcgcgaagg cacgtctgcc
      121 ggacccgctc cgcactggcc acggtcggct ggaccacccg gcctcccacg atcccgacac
      181 cgacgagcgt gtctccgacg acggcgaacg acaccaggtc ggagccgtcc tcgagcagcg
      241 cctcgcgcag ccgggagact ccgagcggtt ggcgctcgcg tccggacgcg ggctgggcgg
      301 cggcggtcca gccgtgctcg cggatctcgc gctggagctg ggcggcccgg tcgagcacgt
      361 cgagggacgg ggcgccacga cggatctgct cctggacgtc gcgaagcgaa gccatgagct
      421 cggccacggc cgggtcggcc gcccggacgc tggggagccg cccggtcgcc gagcgccagc
      481 gttccgtgct ggcaagaaga gcagaagggg agcgggccag ggcaaggtgc atgtcctgca
      541 ccgcgagctg gaccccatgg actgccgtcg cggtacgcag gtccaggctc gcagtgcgcg
      601 actgcgcgcg gcgcaggtct gtggccgccg tgcgcagaac cgtcgcggcg cgggtctgtt
      661 cgccccgcga cgcgtgcagt gtcgcctcga cgagtgagcg gtgcaggcgg ggcgtcaggc
      721 tggtgctgga ctgcacctgg gccagcgcac cctcggcgtc cacgacagcc cctgccgcca
      781 gccacgcgtc ggcctcgagc aggcgggcgt cgaagaccag cgccgggtca ccggcgagcc
      841 cggcccgctc ggcgacccgg cgtgccgtga gcgcgatcga acggggccgg cggctgcggg
      901 gcagctcgat cccgagggcg acgagctggg cacgggccgc ccagaccggc gctcgccgcc
      961 gggcgaacac ggcctgagcg cggcgggcca gcgcggcggc cgacgacggt tcgccgagca
     1021 gcgccagcgc tcgggccagg tcgagctcga tctcgctgtg gacgtgctgc tggcggcgct
     1081 cggccccgag cgcggccgcc tcgcgcagcc gctgggcggc ttcgctgatc agcccggcat
     1141 cgagcaggac cctgccctgg tcatgccagg ccgcggcccg ggagacgtcg gtgtcgatcc
     1201 ggtccgcctc cgccatgagc tcgagcgccg cggccaggtc accggcaagg tatgccgtgt
     1261 ggcccaggtt gtggaccgcc atgaactcct ggcggttcag accgtggcgg gccgcgatct
     1321 gccgggagcg cccgaactcc gctcgcgccg tggatatctc accacgatgg agcatcagca
     1381 ttcctcggtt gttgagcagc acgcactggt cccgagggcc gagcgcgtcg accgagtcgg
     1441 ctgcccggtc cagcgcgtcg agcgcggcgc cgaggtcacc actgcgagcg agcaccatgg
     1501 cgcgctgcga gtgggcgagc gcggtgagct cgtcgtcccc gacggcgcgg gcggcctcga
     1561 cggccgcccg gagcaccggt agcgccgcac ggcggcccga gcgctcgaac tcggcgctgc
     1621 tcagtgccag caggacacgg atccgtgccc gttcggcggc aatggagagg ggctcggggc
     1681 cgtcgaggat gcgcaaggca gcacgaagga accgagctgc ttgggccggg cgccctgccg
     1741 agttggcggc gctcccacgg tcgcgcagct caaggacccg ggcgagggcg gtctcgatgg
     1801 tggccgtggc gccaccctac cgagcacaca ggccagggcc tcggcccctc ggtggtgctg
     1861 agacggtgtg ggacgagatc tggagcagat caggactaga tcgtgatccc gggggtgacg
     1921 actggagtgg ccgccgggct cgccggatcg ggccagatga cgaactgcac ccggccgtgc
     1981 gggacgccct cgacgctgat ccgtccgtgg gcgtcggcca cgagttccgt cgcccagtcg
     2041 tcgacgctga cctcgatctt ggcccggggc gcggtgaccc agccgtcgat acgtgaggtc
     2101 tccccggctc cggccgtgat cgtcaccatg aggctgagcg agccggccgt gaaggtcacc
     2161 gtgtcggcca tggcggtgtc gtcccgggtc gggagcagcg ccatcgtcgt cagctcggcg
     2221 atctcggcgt cgagggcgcg cacggtgagt gcgaacttga tccgctcgac gaggtcgacg
     2281 ggcaccgggt cgaggcggga ctgggtctcg cgaagagtcg ccaggatcag gaagtccacg
     2341 tcgtcgaggc gctcaggcat cgctcacacg ctccatctag agtcggcggc cagggcactc
     2401 cgcagcttgg tcaggcaccg tccccgagtc gggccaatgc tcccgaccgg catccccaag
     2461 gactgcgcaa ggctcgcgta gtccggccgg tcgacgaacg caatcacttc gagcagctcc
     2521 cggcagcggt ccggaagcgc cctcacgtgt ccccacagga tccgctggtg ctcggtgagg
     2581 atgctcagcg cctccggtcc ggggtcgctg ctcacctcct cggggacgac atcgacgggt
     2641 atggtgcgcc ggtcctggcg catgagccgc caggcctcgc gcttgaccgt gacgaccatc
     2701 catgacggca ctcccgcggg gtccttgatg gtgtcggtca ggtcgacgag ctgggtccag
     2761 gccagctgga cggcgtcctc ggcgagcgcg accgatgcgc cctgggcccg ggccgtccgc
     2821 cagagcacgg gggtgagcag gtcgacgagg tcgcccatgc gctggcggtc gcccgcacgg
     2881 taggcgtcga aggcgagggc ggcgcggcca ccgagggtct cccgggtcgg accgctcggg
     2941 gtcacggcct ggtccatggc agctcctcct ccagcgcccg ccagccgcgc tcgagcgccg
     3001 aggcgaggtc gggcttgccg atgtcggggt cggccgcgag atgggccgcc agctcaccgg
     3061 ccagcactgg ggcggcgaag gacgtgccac tccagatccc gaagccaccc gagaggtcgt
     3121 cggggtcgac ggtcgcgcgg ggc
//
LOCUS       DATAIX010000376         3126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_169998, whole genome shotgun sequence.
ACCESSION   DATAIX010000376 DATAIX010000000
VERSION     DATAIX010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169998"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..775)
                     /locus_tag="VHM65_05675"
     CDS             complement(125..775)
                     /locus_tag="VHM65_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2705228.1"
                     /translation="MAVPRDRAETGPHRARMTGKERREQLIEVGRHLFAEKGMEATTV
                     EEIAAKAGVSKPVVYEHFGGKEGLYAVVVDREILALLDGITSALSSAAPARVLLESAA
                     MALLNYVEGSADGFRILVRDSPTGQSTGSFATLISDVATQVEHILAAEFRRRKLDPKT
                     APIYAQMLVGMVALTGQWWLDSRRFKKADVAAHLVNLAWNGLVGLEAKPSLRTTAQ"
     gene            complement(810..1763)
                     /locus_tag="VHM65_05680"
     CDS             complement(810..1763)
                     /locus_tag="VHM65_05680"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX2705229.1"
                     /translation="MTTYAPGPTRSADTGHSTSFPVERKASAEQVALFLFITIPFLAV
                     LLAVPFAWGWGLSWVDVAISLVFFVISSHGITVGFHRYFTHGSFKAVRPLKIALAVAG
                     SMAIEGPVVRWVADHRRHHAFSDRAGDPHSPWRYGETVPALVKGLWWAHIGWLFDVEQ
                     TPRRKYAPDLMDDPDLQKVDHAFLWIVAGSVLFPPLIGGLLTWSWQGAVTAFFWASLV
                     RVALLHHVTWSINSICHAMGSRPFLSRDRSGNVWWLALLSNGESWHNLHHADPTCARH
                     GVEPGQIDSSARMIGLFERFGWASDVRWPRQERLDARRAVT"
     gene            1915..1993
                     /locus_tag="VHM65_05685"
     tRNA            1915..1993
                     /locus_tag="VHM65_05685"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1950..1952,aa:Gln,seq:ttg)
     gene            2087..>3126
                     /locus_tag="VHM65_05690"
     CDS             2087..>3126
                     /locus_tag="VHM65_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX2705230.1"
                     /translation="MTQPRPVAVIVLAAGEGTRMKSATPKVLHEIGGRSLVGHALAAA
                     RETGPEHLVVVVRHERDLVAAHLAKIAPEALVADQDELKGTGRAAECGLEVLSGDLVG
                     TVLVTMGDVPLLSGQTLRDLTVAHEDTGSAVTVITAELDDPTGYGRVVRDHAGGVLGI
                     VEHKDATEEQRAIREVSSGIWAFDLEVLRAALAQVGTDNSQGEKYLTDVLAIARSAGL
                     STAAHHIADLWQTEGVNDRVQLARLGAELNRRTVEHWMREGVTVVDPATTWIDADVSI
                     GRDTRILPGTQLLGATSVGGGALIGPDTTLADVEIGDGASVIRTQGSHALIGAGASVG
                     PFAYLRPGTELGA"
BASE COUNT          494 a         1063 c         1121 g          448 t
ORIGIN      
        1 atgtcgtggt cggcgatgtc gtggtcgccg accacgacat cgccgacctc gaccacgacg
       61 gcgcccgagg cgccagcgac gacagcggtc cggccggcgg acgtggccgc cgggctggtc
      121 ctggctattg ggccgtggtt cgcagtgacg gcttggcttc gagaccgacg aggccgttcc
      181 aggcgagatt gacaaggtgg gccgcgacat cggccttctt gaaccggcgc gagtcgagcc
      241 accattgtcc ggtgagcgcc accatgccga cgagcatctg ggcatagatc ggcgcggtct
      301 tcgggtccag cttgcgccgc ctgaactcgg ccgccaggat gtgctcgacc tgcgtggcca
      361 catcgctgat cagcgtggca aaggaccctg tcgactgacc cgtcggggag tcgcgcacga
      421 ggatccggaa gccgtcggcc gacccttcga catagttgag cagcgccatc gccgccgact
      481 cgaggaggac acgagccggc gccgcgctcg agagagcact ggtgatgccg tcgagcaggg
      541 cgaggatctc gcggtccacg acgacggcat acaggccctc cttgccgccg aagtgctcat
      601 acacgaccgg cttggacaca ccggccttgg cggcgatctc ctcgaccgtc gtcgcctcca
      661 tccccttctc ggcgaagagg tgccggccga cctcgatgag ctgttcgcga cgttccttgc
      721 cggtcatccg ggcccgatga ggtcccgtct cggcacggtc gcgtggtact gccatggctc
      781 catcatgccg gtcgatgagg aggaccggct caggtcacgg cgcggcgggc gtccaggcgc
      841 tcctggcgcg gccaacggac gtcgctggcc cagccgaacc gctcgaacag cccgatcatc
      901 cgggccgagc tgtcgatctg gccaggctcg accccgtgcc gggcgcaggt cgggtcggca
      961 tggtgcaggt tgtgccacga ctcaccgttg gagagcagcg cgagccacca gacgttgccg
     1021 gagcggtcac ggctgaggaa cggccggctg cccatcgcgt gacagatcga gttgatcgac
     1081 caggtgacgt ggtggagcag ggccacccgg accagcgagg cccagaagaa ggcggtcacc
     1141 gcgccctgcc aggaccaggt cagcagaccg ccgatgaggg gcgggaagag caccgagccg
     1201 gcaacgatcc acaggaaggc gtggtcgacc ttctgcaggt cggggtcgtc catgaggtcg
     1261 ggggcgtact tgcgccgtgg cgtctgctcg acgtcgaaga gccagccgat gtgcgcccac
     1321 cacagcccct tgaccagcgc ggggaccgtc tcgccatacc gccaggggga gtgcgggtct
     1381 cccgcgcggt cggagaaggc gtggtggcgg cggtggtcgg cgacccagcg cacgaccggg
     1441 ccctcgatcg ccatcgagcc ggcgacggcg agggcgatct tgagcgggcg cacggccttg
     1501 aacgacccgt gggtgaagta gcggtggaag ccgacggtga tgccgtggct cgagatgacg
     1561 aagaacacca gggagatcgc cacgtcgacc cagctcaggc cccagcccca cgcgaacggg
     1621 accgcgagca gcaccgcgag gaacgggatc gtgatgaaca ggaagagtgc gacctgctcg
     1681 gcgctcgcct tgcgttcgac cggaaaggag gtcgagtggc cggtgtcggc cgagcgggtc
     1741 gggcctgggg cgtatgtcgt catggtgcgc cttcgtctcg ggaggtaacc tacgcaaccg
     1801 taacctacgt ttgcgtaagt tccaaccggc gtttcgggaa gtggacgcac gcgcactacc
     1861 ctggctgagt cgtcagccct cgacgtcagg tatggcgggc gggtgcggcc acgttccccg
     1921 gttggtctgc tggtagggcc cgcctgactt tgaatcagga ctagcgtcgc aggttcgatt
     1981 cctgcccggg gagccgtggg gggctcgcgg tgaggtcccc atgaccaggg ccgctacgct
     2041 gggcgagcct cggtagtgat cgtccagcga taggggagcc ctcgtcgtga cccagccacg
     2101 gccggttgcc gtcatcgtgc tcgccgcagg tgaaggcact cggatgaagt cggcgactcc
     2161 caaggtgctg cacgagatcg gagggcgcag cctggtcggc cacgccctgg cggccgcccg
     2221 cgagacgggg ccggagcacc tggtcgtcgt cgtccgccac gagcgtgacc tcgtcgccgc
     2281 ccacctggcc aagatcgccc ccgaggcact cgtggccgac caggacgagc tcaagggcac
     2341 gggccgcgcc gccgagtgcg gtctcgaggt gctctccggg gacctggtgg gcaccgtcct
     2401 ggtcaccatg ggcgatgtcc cgctgctgag cgggcagacc ctgcgggatc tcaccgtcgc
     2461 ccatgaggac accggctccg cggtcacggt catcaccgcc gagctcgacg accccacggg
     2521 ctacgggcgg gtcgtccggg accacgcggg gggggtgctc ggcatcgtcg agcacaagga
     2581 cgccaccgag gagcagcgcg ccatccgcga ggtcagttcc gggatctggg cattcgacct
     2641 cgaggtcctg cgggctgccc tggcccaggt cggcaccgac aacagccagg gggagaagta
     2701 cctcaccgac gtcctggcca tcgcccgctc ggctgggctg agcaccgccg cacaccacat
     2761 cgcggacctc tggcagaccg agggagtcaa cgatcgggtc cagctggccc gcctcggcgc
     2821 cgagctcaac cggcgcaccg tcgagcactg gatgcgtgag ggcgtcaccg tcgtcgaccc
     2881 ggcgaccacc tggatcgacg ccgacgtcag catcggccgg gacacccgga tcctgcccgg
     2941 cacccagctg ctcggcgcca cgtcggtcgg cggcggcgcc ctgatcggcc ccgacacgac
     3001 cctggccgac gtcgagatcg gtgacggcgc gagcgtcatc cggactcagg ggtcccacgc
     3061 gctcatcggg gcgggcgcgt cggtcggtcc cttcgcctac ctgcgcccgg gaaccgagct
     3121 cggcgc
//
LOCUS       DATAIX010000377         2753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_80765, whole genome shotgun sequence.
ACCESSION   DATAIX010000377 DATAIX010000000
VERSION     DATAIX010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80765"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VHM65_05695"
     CDS             <1..734
                     /locus_tag="VHM65_05695"
                     /inference="COORDINATES: protein motif:HMM:NF037564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3048 C-terminal domain-containing protein"
                     /protein_id="HEX2705231.1"
                     /translation="HSQLPARVGPIRSVRPMDAPLLGPLNPVFGNTMGARWVMAYVDR
                     VAHLDDLGTLRVKARSGAYVLDSRRVAPNHVFAQPAELMALSRLRKPPAPYFTVAKEA
                     VAASTAGGSAARSADIPYGPKWDVKWAYDAGKAIYLRSQPWGRHVMADGRQIFATNVL
                     VLRVGSDLGKIGTASGAPVPILDLIDGSGSLVLLSQGRQVKGTWTKAGVNDPFVLTAK
                     GKPLLLAPGNTWVEMPTPDAKVILR"
     gene            complement(808..2109)
                     /gene="paaK"
                     /locus_tag="VHM65_05700"
     CDS             complement(808..2109)
                     /gene="paaK"
                     /locus_tag="VHM65_05700"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: protein motif:HMM:TIGR02155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HEX2705232.1"
                     /translation="MTTTARRTDPALAWSVDELREAQLTRLQDTLQRTYDGVAYYRSA
                     WVAAGVHPRDIRTLEDLRYLPMTAKADLRDNYPFGLFAVPREQVVRIHASSGTTGKPT
                     VVGYTQADVDMWADVVARSIAFAGGRPGDACHIAYGYGLFTGGLGAHYGAERLGCTVI
                     PISGGMTERQVQLIADFQPRIIMVTPSYFLSLLDEMDRQGLEPMTSSLEIGIFGAEPW
                     TEAMRVEIEARSGIDAVDIYGLSEVIGPGVAQESGTTKDGLHIWEDHFYPEIIDPVTE
                     KVLPDGEQGELVFTSLTKQAMPIIRYRTRDLTRLLPGTAAPGYRRMQKVTGRTDDMMI
                     IRGVNVFPTQIEEHVLSEPALAPYFQCILTQPGNMVELTVLVEPHQRMSHPDKLDISR
                     RLQHRIKATIGISCTIDVQDPGRIERSIGKARRLIDKRKEE"
     gene            complement(2215..>2753)
                     /locus_tag="VHM65_05705"
     CDS             complement(2215..>2753)
                     /locus_tag="VHM65_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX2705233.1"
                     /translation="LNDKRIAAQRRDIGMVFQRFNLFPHKTALENIMEAPVQVKGEKK
                     REAEHRALELLDRVGLKDKPAAYPAQLSGGQQQRVAIARALAMRPKLMLFDEPTSALD
                     PELVGEVLDVMRELAQDGMTMIVVTHEMTFARDVADEVVFIDGGVVVEQGNPKDVITN
                     PQHQRTKTFLRRILAEDE"
BASE COUNT          396 a          905 c          969 g          483 t
ORIGIN      
        1 tccactccca gctgccggcc cgggtcggcc cgatccggtc ggtccgaccg atggacgccc
       61 cgctgctcgg accactcaac cccgtgttcg gtaacacgat gggtgcccgc tgggtcatgg
      121 cgtatgtcga ccgggtggcc cacctggacg acctcggcac gcttcgggtg aaggccaggt
      181 ccggtgcgta tgtcctggac tcccggcggg tcgcgcccaa ccacgtcttc gcccagcccg
      241 ccgagctcat ggccctgagc aggctgcgca agccccctgc gccctacttc accgtcgcca
      301 aggaagccgt cgcagcgtcg accgccgggg gttctgcggc gaggtcggcc gacatcccgt
      361 atggcccgaa gtgggacgtg aagtgggcct acgacgccgg caaggcgatc tacctgcgca
      421 gccagccgtg gggcaggcac gtcatggccg acggccggca gatctttgcc accaacgttc
      481 tcgtcctcag ggtcggctcg gacctcggca agatcgggac cgcctcgggg gcgcccgtgc
      541 ccattctcga cctcatcgac ggctccgggt cgctggtgct gctctcccag ggacgccagg
      601 tcaagggcac ctggaccaag gcgggggtca acgacccgtt cgtgctcacc gcgaagggca
      661 agccgctcct gctcgctccc ggcaacacct gggtcgagat gccgacgccc gacgcgaagg
      721 tcatcctgcg ctaggcggga cgcagggacc gctcgcgtac tggttgcggg ccgggggctg
      781 gcgcgcgtca gggcgttggc gccgaggtca ttcctctttg cgcttgtcga tgagccggcg
      841 ggccttgccg atcgagcgct cgatcctgcc gggatcctgc acgtcgatcg tgcacgagat
      901 gccgatcgtg gccttgatcc ggtgctgcag ccgtcgggag atgtcgagct tgtcggggtg
      961 gctcatccgc tggtgtggtt cgacgagcac ggtgagctcg accatgttgc cgggctgggt
     1021 gaggatgcac tggaagtacg gcgcgagcgc gggctcggag aggacgtgct cctcgatctg
     1081 ggtcgggaag acgttgacac cgcggatgat catcatgtcg tcggtgcgcc cggtgacctt
     1141 ctgcatccgt cggtaacctg gcgccgccgt gcctggcagg agccgggtca ggtcccgcgt
     1201 gcggtagcgg atgatcggca tcgcctgttt ggtcagcgag gtgaagacga gctcgccctg
     1261 ctccccgtcg gggaggacct tctcggtgac cgggtcgatg atctccgggt agaagtggtc
     1321 ctcccagatg tgcagcccgt ccttggtcgt cccagactcc tgggccacgc cggggccgat
     1381 cacctcggag agcccgtaga tgtcgacggc gtcgatcccg gaccgcgcct cgatctccac
     1441 gcgcatcgcc tcggtccacg gctcggcgcc gaagatcccg atctccagcg aggaggtcat
     1501 gggctccagc ccctggcggt ccatctcgtc gagcagcgag aggaagtagc tcggggtgac
     1561 catgatgatg cgcggctgga agtccgcgat gagctggacc tggcgctccg tcataccccc
     1621 ggagatcggg atgaccgtgc agccgagccg ctcggcgccg tagtgggccc cgagtccgcc
     1681 ggtgaacagg ccgtaaccgt aggcgatgtg gcaggcatcc ccagggcgcc cgccggcgaa
     1741 ggcgatcgag cgcgcgacga catcggccca catgtcgacg tcggcctggg tgtagccgac
     1801 gacggtcggc ttgccggtcg tgccactgga ggcgtggatg cggacgacct gttcgcgcgg
     1861 gacggcaaag agcccgaacg ggtagttgtc gcgcaggtct gccttggcgg tcatcggcag
     1921 gtatcgcaga tcctcgagag tccgtatgtc gcgcgggtgc actcccgctg cgacccaggc
     1981 cgagcggtag taggccacac cgtcatacgt gcgctggagg gtatcctgta gccgcgtgag
     2041 ctgcgcctct cggagttcgt cgaccgacca cgcgagggcg gggtcggtcc tgcgtgccgt
     2101 cgttgtcatg ctgacctccg gtcgggcgct actgtcctta ccgcgatcct gccgtgaggg
     2161 ggtgctgtcc gcaacgacag atgcggaaca tgatgtagct actcggtgac gtggctactc
     2221 gtcctcggcg agaatccggc gcaggaacgt cttggtccgc tggtgctgcg ggttggtgat
     2281 gacgtccttg gggttgccct gctcgacgac gaccccaccg tcgatgaaga cgacctcgtc
     2341 ggcgacgtcg cgcgcgaagg tcatctcgtg ggtgacgacg atcatcgtca tcccgtcctg
     2401 ggcgagctcg cgcatgacgt cgagtacctc cccgaccagc tcggggtcca gggcagaggt
     2461 cggctcgtcg aagagcatga gcttggggcg catcgccagc gcccgggcga tcgcgacacg
     2521 ctgctgctga ccgccggaga gctgcgccgg gtaggccgcg ggcttgtcct tgaggccgac
     2581 ccggtcgagc agctcgagcg cgcggtgctc ggcctccctc ttcttctcgc ccttgacctg
     2641 gaccggtgcc tccatgatgt tctcgagcgc ggtcttgtgc gggaagaggt tgaaccgctg
     2701 gaagaccatc ccgatatctc ggcgctgggc ggcaatccgc ttgtcgttca gcc
//
LOCUS       DATAIX010000378         2900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_80803, whole genome shotgun sequence.
ACCESSION   DATAIX010000378 DATAIX010000000
VERSION     DATAIX010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80803"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..2784)
                     /gene="pepN"
                     /locus_tag="VHM65_05710"
     CDS             complement(124..2784)
                     /gene="pepN"
                     /locus_tag="VHM65_05710"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEX2705234.1"
                     /translation="MGEIQCPAEPGTLPPFRRVNPVPGTNLTREEALTRAALVHVDRY
                     DVELDLTTGAETFRSVTTVHFTCARPGAQTFIDLVSASVDAVTLNGAALDPGVHADGH
                     RVTLPGLQAGNVLVVEATCRYMNTGEGLHRFSDPVDGEVYLYSQFEVADSRRVFAVFE
                     QPDLKAEFAFTVTAPAHRQVISCSAYPEPEPLPSDPEPLSEPGSATRPAARWTFSPTP
                     RLSSYVTAIHAGPYAVVHDSVQTRRGVVPLRILCRASLLPHLDAGAIFDVTKRGFAFF
                     EEEFDYAYPFDTYDQVFAPEYNVGAMENAAAVTISEIYVFRSQVAEMRIERRALTILH
                     ELAHMWFGDLVTMRWWDDLWLNESFAEWASTACQAEATRWASSWTTFALTEKTWAYAQ
                     DQLSSTHPIRADMRHLEDVESNFDGITYAKGAAALKQLVAFVGRTAFRDAVRAYFAAH
                     AWSSTELGDLTAELERASGRDLGHWAQAWLETAGVTTLAARFEVGPDGRYTSFAVEQT
                     ATADLPTLRPHRFGIGRYDVRDGRLVRVGYDEVDIDGPLTHLPAFVGAAQPDLLLLND
                     EDLTYTVIRLDERSLATALAHPRGFTDSLPAAVVLGAAWDMTRSAEMPARSFLALALA
                     LDSLPGETNSTMLSLLLSEIQSAVLYYVTPPRRDETRRLAAQRLRELALAAEPRGDAQ
                     HQLIRAYAAMATDDEAAAHLQSLLDGTSDIEGVDIDTELRWLLLRALASLGQVDAEAI
                     AAEAARDATATGHEREAQTLAARPTAEAKAQAWTRAVEHTDATNAIVEALALGFVRTP
                     GTSSLEPYVERYHDALLGVWSTRTVAVATIVVKGFYPMPLAGQPLLDATQAWLDAHPD
                     APAGLLRLVGENRDQVARAVAAQALDATLG"
BASE COUNT          402 a          955 c         1130 g          413 t
ORIGIN      
        1 tcacggacgg gcggggacgc ctcgtgtcgg tggagcagct gttcacccac cggctggctg
       61 ggaggatcgc cagggccgtg gccggagcct gacctccatc ggtgcgtccg ctcggttggg
      121 cgctcagccc agggtcgcgt cgagggcctg ggccgccacg gcccgggcga cctgatcccg
      181 gttctcaccg acgaggcgca gcagccccgc gggcgcgtcc gggtgggcgt cgagccaggc
      241 ctgggtcgcg tcgagcagcg gctgaccggc gagcggcatc gggtagaacc ccttgacgac
      301 gatggtcgcg accgcgacgg tccgggtcga ccagacgccg agcagggcgt cgtggtagcg
      361 ctcgacgtac ggctcgaggc tcgacgtgcc gggggtgcgc acgaagccca gggccagggc
      421 ttcgacgatc gcgttcgtcg cgtcggtgtg ctcgaccgct cgggtccagg cctgcgcctt
      481 ggcctcggcc gtgggccgcg ccgccagcgt ctgcgcctcc cgttcgtgcc ccgtggccgt
      541 ggcgtcacgg gcggcttcgg cggcgatggc ctcggcatcg acctggccga gcgacgccag
      601 cgcccgcagg agcagccagc gcagctcggt gtcgatgtcg acgccctcga tgtccgaggt
      661 cccgtcgagc agcgactgca ggtgagccgc cgcctcgtca tcggtcgcca tcgcggcata
      721 ggcgcggatg agctggtgct gggcatcgcc gcgcggctcg gcggcgaggg cgagctcgcg
      781 cagccgctgg gccgccagcc ggcgggtctc gtcgcgccga ggcggggtga cgtagtacag
      841 gactgcggac tggatctcgc tcaacagcag ggacagcatg gtcgagttcg tctcgccagg
      901 gaggctgtcg agggcgaggg cgagggcgag gaagctccgg gcgggcatct cggccgaccg
      961 ggtcatgtcc cacgcggcgc cgagcacgac ggccgcgggg aggctgtcgg tgaacccccg
     1021 gggatgggcc agggcggtcg ccagcgaccg ttcgtcgagg cggatcacgg tgtaggtgag
     1081 gtcctcgtcg ttgagcagga gcagatcggg ctgggcggcg cccacgaacg cgggcagatg
     1141 tgtcagcggg ccgtcgatgt ccacctcgtc gtagccgacc cgcacgagcc ggccgtccct
     1201 gacgtcatac ctgccgatcc cgaagcggtg cgggcgcagc gtcggcaggt ccgctgtggc
     1261 tgtctgctcg acggcgaacg aggtgtaccg gccgtcgggt ccgacctcga accgcgcagc
     1321 gagcgtcgtg actcccgccg tctcgagcca ggcctgcgcc cagtggccca ggtcccgtcc
     1381 ggaggcccgt tcgagctcgg cggtgaggtc gcccagctcg gtgctggacc aggcatgggc
     1441 cgcgaagtag gcgcgcaccg cgtcacggaa ggcggtgcgc ccgacgaagg cgacgagctg
     1501 cttgagcgcg gccgcgccct tggcataggt gatgccgtcg aagttgcttt cgacgtcctc
     1561 gaggtggcgc atgtcggccc ggatcgggtg ggtcgaggag agctggtcct gggcgtaggc
     1621 ccacgtcttc tcggtgagcg cgaaggtcgt ccacgagctc gcccaccggg tcgcctcggc
     1681 ctggcacgcc gttgaggccc actccgcgaa ggactcgttg agccagaggt cgtcccacca
     1741 gcgcatggtc acgaggtcgc cgaaccacat gtgcgccagc tcgtgcagga tcgtcagggc
     1801 ccgccgctcg atgcgcatct cggcgacctg ggagcggaag acgtagatct cgctgatcgt
     1861 caccgcggcg gcgttctcca tcgcgcccac gttgtactcc ggcgcgaaga cctggtcgta
     1921 ggtgtcgaac gggtaggcgt agtcgaactc ctcctcgaag aaggcgaacc cgcgcttggt
     1981 cacgtcgaag atcgcgccgg cgtcgaggtg gggaagcagg ctggcccggc acaggatccg
     2041 cagcggcacg acgccacggc gagtctgcac actgtcgtgc acgacggcat acgggcccgc
     2101 atgaatcgcc gtgacatatg acgacaggcg cggcgtcggg gagaaggtcc agcgggccgc
     2161 gggccgggtc gccgacccgg gctcgctcag cggctcgggg tcgctgggca ggggctcggg
     2221 ctcggggtac gcggagcacg agatgacctg cctgtgcgcc ggcgcggtca cggtgaaggc
     2281 gaactcggcc ttgaggtcgg gctgctcgaa caccgcgaag acgcgccgcg agtccgccac
     2341 ctcgaactgc gagtagaggt agacctcgcc gtcgaccggg tcggagaagc ggtgcagccc
     2401 ctcgccggtg ttcatgtacc ggcaggtggc ctcgacgacg aggacgttgc cggcctgcag
     2461 gcccggcagg gtgacgcggt ggccgtcggc gtgcacgccg gggtccaggg cggccccgtt
     2521 gagggtgacc gcgtcgacgg aggcgctgac gaggtcgatg aacgtctgcg cgcccgggcg
     2581 ggcgcaggtg aaatggacgg tcgtcacgct gcggaacgtc tcggcgccgg tcgtgagatc
     2641 gagctcgacg tcgtagcggt cgacgtggac cagggcggcg cgggtcagcg cctcctcacg
     2701 ggtgaggttg gtgccgggca cggggttgac tctcctgaac gggggcaggg tgccgggctc
     2761 agccgggcac tggatctcgc ccatcctcgc acggggtgag cgccgcgcga gcctggatcg
     2821 agcagcgcgc cgccccggat cggcgctcgg tcagcacggc gccgtggttg aatcccggta
     2881 tgacgacacc ggccgagatg
//
LOCUS       DATAIX010000379         1638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_217647, whole genome shotgun sequence.
ACCESSION   DATAIX010000379 DATAIX010000000
VERSION     DATAIX010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217647"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /locus_tag="VHM65_05715"
     CDS             <1..1509
                     /locus_tag="VHM65_05715"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX2705235.1"
                     /translation="GGDPVSMPARAEVRHTSHDRLVADVAALLADGWRLALAAAHEDP
                     EGFRVVYTFLRQRAAPVELWVTRPREQAWLPTLAALSYPAGRFEREMRDLYGIEPRDH
                     PMPRRLVRHGHWPVGYYPMLRNADPEPRFDEDVGSYPFVEVEGEGVYEIPVGPVHAGL
                     IEPGHFRFSVVGETILRMKARLWFLHRGVEKLLEGRAPEQGIELAERVSGDTAVGHSL
                     AFVMAVEQARGVEVPPRALAVRALLLELERLYNHVSDIGAIVNDVGYGIVHAHTQRLR
                     EQLLRHNKHVTGHRLLRGAVVIGDAGLSGEPDLGLIGDVARATAELVDIALGNHVVND
                     RLTGTAVLDTEQARLLGTLGYVARASGLEGDARLDHPFVGLPAGFEPAVQTSGDVMAR
                     FLVRADEVRAGAALVAAYAEAARTASPSVPATDRAVAPAESHGFGVVEAWRGALCQRV
                     ELAPDGTLSRVKIVDPSFFNWPALPVALADTIVPDFPLANKSFNQSYAGNDL"
BASE COUNT          208 a          594 c          592 g          244 t
ORIGIN      
        1 gggggtgacc cggtgagcat gcccgcgcgc gccgaggtgc gtcatacctc ccacgaccgg
       61 ctggtcgccg acgtggcggc gctgctcgcc gacggctggc ggctcgccct ggccgcggcc
      121 cacgaggacc ccgaaggctt ccgggtcgtc tacaccttcc tgcggcaacg cgcggcgccg
      181 gtcgagctct gggtcacccg cccccgcgag caggcgtggc tgccaacgct cgcagcgctg
      241 tcctatcccg ccgggcggtt cgagcgcgag atgcgcgacc tctacgggat cgagccgcgg
      301 gaccacccga tgccgcggcg cctcgtgcgg cacgggcact ggccggtggg ttactacccg
      361 atgctgcgca atgccgaccc cgagccacga ttcgacgagg acgtggggtc ctacccgttc
      421 gtcgaggtcg agggggaggg ggtctacgag atcccggtcg ggccggtcca tgccggactg
      481 atcgagccgg ggcacttccg gttctcggtc gtcggcgaga ccatcctgcg gatgaaggcg
      541 cggctgtggt tcctccatcg cggggtcgag aagctcctcg aaggacgggc tcccgagcag
      601 ggcatcgagc tggccgagcg ggtgtcgggg gacacggcgg tcggccactc gctggccttc
      661 gtcatggccg tcgagcaggc gcgaggggtc gaagtgccgc cgcgagcgct ggcggtgcgg
      721 gcgctgctcc tcgagctcga gcgcctctac aaccacgtca gcgacatcgg ggcgatcgtc
      781 aacgacgtcg ggtacgggat cgtccatgcc catacccagc ggttgcgtga gcagctgctg
      841 cgccacaaca agcacgtcac cgggcaccgg ctgctccggg gcgctgtcgt gatcggcgat
      901 gccggcctga gcggggagcc cgacctcggg ctcatcggcg atgtggcccg cgccaccgcc
      961 gagctcgtcg acatcgccct cggcaaccac gtcgtcaacg accggctcac tggcacagcg
     1021 gtgctcgaca ccgaacaggc ccggctcctg ggcacgttgg gctacgtcgc ccgtgcgtca
     1081 gggctcgagg gtgacgcccg cctggaccat ccgttcgtgg gcctgcccgc cgggttcgag
     1141 ccggcggtcc agacgtccgg ggatgtcatg gcccggttcc tcgtgcgcgc cgacgaggtg
     1201 cgggccggcg cggccctggt cgcggcgtat gccgaggcgg cccggaccgc ctcgccgtcg
     1261 gtgccggcga ccgaccgggc ggtggccccg gccgagagcc acgggttcgg tgtggtcgag
     1321 gcctggcgcg gggctctgtg tcagcgggtc gagctcgccc ctgacgggac gctgagccgg
     1381 gtcaagatcg tcgacccgtc gttcttcaac tggccggccc tgccggtcgc cctggccgac
     1441 acgatcgtcc ccgacttccc cctggccaac aagagcttca accagtccta cgcgggcaac
     1501 gacctctgat cctgcggcgg gcccgtcgta ctgacgggca cggccccacc tggttgcgca
     1561 gacgcgcaac caggttggtc gcgttcttgt cacgtagccc gccggcgtcc tcgcccgcgt
     1621 cctcgcccgt gcccccca
//
LOCUS       DATAIX010000380         3185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_20756, whole genome shotgun sequence.
ACCESSION   DATAIX010000380 DATAIX010000000
VERSION     DATAIX010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20756"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VHM65_05720"
     CDS             <1..722
                     /locus_tag="VHM65_05720"
                     /inference="COORDINATES: protein motif:HMM:NF028211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX2705236.1"
                     /translation="LPQHDGLTYVGLTDEPLGTDVIPDVPEPAEGEVGFLLDVVSSVL
                     AEPLSRADVLGAYAGLRPLLDASGRTADLSRRHAVLTSPDGVVTVVGGKLTTYRRMAQ
                     DAVDTVVGQRSLAAGPCRTRRLPLVGAGDTRALRATGDPARLPARLIDRYGVEAGAVA
                     ASPDLLAPISASIPVTHAELRWGVEHEGALDVDDLLDRRTRIGLVPADRARAIPAALA
                     ALRSPDGTGPGATAWGSTGTA"
     gene            complement(758..1570)
                     /locus_tag="VHM65_05725"
     CDS             complement(758..1570)
                     /locus_tag="VHM65_05725"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEX2705237.1"
                     /translation="MDGGLVARSARGAIGEHWWSRRFLEVLESFALGSRLTRGKNYAR
                     RGQVISMKVAPGVVTASVQGSRRTPYKVSIGLPAFSELVWAKVEVTLAEQAIHSARLL
                     AGEMPHDLEEVFSEAGAPLFPQRATDLTLSCSCPDWEVPCKHLAATFYLLAESFDDDP
                     FAILLWRGRRREALLNRLRELRGGEPLPAAEQAAVRTETPRVGAMMALADVSVAAGDF
                     WTGAPVPALPVLAELPTDLLLRQLPVPGAVLGGPALVTFLTPLYAVLGEAPD"
     gene            complement(1666..>3185)
                     /locus_tag="VHM65_05730"
     CDS             complement(1666..>3185)
                     /locus_tag="VHM65_05730"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2705238.1"
                     /translation="ETQGWLADLLSGQVDHRITPMTAPEGFTGALRPYQERGLAWLSF
                     LESVGLGGVLADDMGLGKTVQLLALMCGELSADVGPTLLVCPMSLVGNWQREAARFAP
                     GLRVHVHHGAERPQGEAFQTVVANSDLVVTTYGLVIRDVDALREMGWRRVVLDEAQAV
                     KNAATRAAVAVRSLPSSHRFAVTGTPVENRLGDLWSLMEFANPGLLGQASSFKARFAT
                     PIERRGDAEAATRLRSLTQPFVLRRLKTDTSIITDLPAKIEMEVVCSLTREQASLYQA
                     VVADMMDRIESTDGIERRGLVLATMTKLKQVCNHPAQFLKDGSRLGGRSGKLARVEEI
                     LDEVLASGEKALLFTQYAEFGDMLRTHLGARFGREVAYLHGGLGKGQRDDLVARFQNP
                     ARGGPSLFVLSLKAGGTGLTLTAANHVIHVDRWWNPAVEDQATDRAFRIGQTRSVQVR
                     KLVCAGTLEEKIAMMIKDKRGLAASVVGTGEGWLTELSTSALRELFTLEAGSVVE"
BASE COUNT          474 a         1203 c         1079 g          429 t
ORIGIN      
        1 gcctgcccca gcacgacggc ctcacctacg tggggctgac cgacgagccg ctcggcaccg
       61 acgtcatccc ggacgtcccc gagccggccg agggcgaggt cggcttcctg ctcgatgtcg
      121 tcagctccgt tctcgccgaa ccgctttcgc gtgccgacgt cctcggcgcg tatgccgggc
      181 tgcggccgct tctcgacgcc tcgggccgca ccgccgacct ctcccgccgc cacgctgtgc
      241 tgacctcccc cgacggtgtc gtcaccgtgg tcggcggcaa gctgacgacc taccgccgga
      301 tggcccagga tgcggtcgac accgtggtgg gccagcgctc cctcgcggcc ggcccatgcc
      361 gcacccggcg gctcccgctc gtcggggccg gcgacacccg tgccctgcgc gccacaggag
      421 acccagcgcg gctcccggcg cggctcatcg accggtatgg cgtcgaagcc ggcgccgtcg
      481 cagccagccc cgacctgctc gccccgatca gcgcgtccat ccccgtgacc cacgccgagc
      541 tgcgctgggg agtcgagcac gagggtgccc tcgacgtcga cgacctgctc gaccggcgca
      601 cccggatcgg cctcgtcccc gccgaccgtg cgcgcgccat acccgccgct ctggcggccc
      661 tgcgctcgcc ggacgggacc ggacccggcg cgaccgcctg ggggagcact ggcaccgcct
      721 gagccggccg tcctggcgga gctctgggcg tctgggctca gtcgggcgcc tctccgagga
      781 ccgcgtacaa cggcgtcagg aaggtcacca gggccgggcc acccagaacc gctcccggca
      841 cggggagctg gcgcagcagc agatccgtcg gcagctcggc caggacaggc agcgccggga
      901 cgggcgcccc ggtccagaag tctccagcag ccacgctcac atcagcgagc gccatcatgg
      961 cccccacacg gggggtctcg gtcctgacgg ccgcttgctc cgctgccggg agaggctcgc
     1021 cgccacgcag ctcgcgaagc cggttcagca gtgcctcgcg gcggcgtccg cgccagagca
     1081 ggatggcgaa ggggtcgtcg tcgaagctct ccgcgagcag gtagaacgtc gccgcgaggt
     1141 gcttgcacgg gacctcccag tccgggcagg aacagctcag cgtcaggtcc gtggcccgct
     1201 gcgggaagag cggcgcgccg gcttccgaaa agacctcctc gaggtcgtgc ggcatctcgc
     1261 cggcgagcag ccgggcactg tggatcgcct gctcggcgag ggtgacctcg accttggccc
     1321 agaccagctc actgaaggcc ggcaacccga tcgacacctt gtatggcgtc cgccgggagc
     1381 cctgcaccga ggccgtcacc acgcccgggg ccaccttcat cgagatcacc tgaccccggc
     1441 gggcgtagtt cttgccgcgg gtcagccggg agccgagcgc gaaggactcc agcacctcga
     1501 ggaagcgccg cgaccaccag tgctccccga tggcacctcg cgcgctgcgg gccacgagcc
     1561 cgccgtccac agcgcgaggc ttggccggtg gcgggaagcc gccggaccag ttcgaacgtc
     1621 cgcgacccgg cggtcccagt ccaccaccga tacgaccggg ggggctcact ccaccaccga
     1681 cccggcctcg agcgtgaaca gctcacgcag ggcggaggtg gagagctcgg tgagccagcc
     1741 ctcaccggtg cccacgacac tggcagccag gccccgcttg tccttgatca tcatggcgat
     1801 cttctcctcc agcgtgcccg cgcacaccag cttgcgcacc tggaccgagc gggtctgccc
     1861 gatccggaag gcacggtcgg tggcctggtc ctccacggca gggttccacc agcggtcgac
     1921 gtggatgacg tggttggccg cggtgagggt gagccccgtg ccacctgcct tcagcgacag
     1981 gacgaacagg gacggcccgc cccgggcagg gttctggaac cgggcgacga ggtcgtcccg
     2041 ctgacccttg cccagtcccc cgtgcaggta ggcgacctca cggccgaagc gggcaccgag
     2101 gtgagtacgc agcatgtcgc cgaactcggc gtactgcgtg aacagcagcg ccttctcccc
     2161 cgaggcgagc acctcgtcga ggatctcctc cacccgcgcc agcttgccgg agcgtccgcc
     2221 gagtctgctg ccgtccttga ggaactgcgc cgggtggttg cagacctgct tgagcttggt
     2281 catggtcgcc aggacgagtc cgcggcgctc gatgccgtcg gtgctctcga tgcggtccat
     2341 catgtccgcg acgaccgcct ggtagagcga ggcctgctcg cgagtgagtg agcacaccac
     2401 ctccatctcg atcttggccg gcaggtcggt gatgatcgag gtgtcggtct tgagccggcg
     2461 caacacgaac ggctgcgtca gcgatctcag ccgggtcgcc gcctcggcat cgccgcggcg
     2521 ctcgatcggc gtcgcgaagc gcgccttgaa ggacgaggcc tgaccgagca gtccggggtt
     2581 ggcgaactcc atcagcgacc agaggtcgcc cagccgattc tcgaccgggg tcccggtcac
     2641 cgcgaaccga tgtgacgacg gcagcgagcg caccgcaacc gccgcccggg tggccgcgtt
     2701 cttcaccgcc tgggcttcgt cgaggacgac gcgacgccag cccatctcac gcagcgcgtc
     2761 gacgtcgcgg atcaccagcc cgtatgtcgt cacgaccagg tccgagttcg ccaccaccgt
     2821 ctggaacgcc tcgccctgag gtcgctcagc cccgtggtgg acgtgcaccc gcagccccgg
     2881 ggcgaagcgg gcggcctcgc gctgccagtt gccgaccagc gacatcgggc agaccagcag
     2941 ggtcggaccc acgtccgcgg agagctcgcc gcacatcagg gcgagcagct gcaccgtctt
     3001 gcccagaccc atgtcgtcgg cgaggacacc gcccagaccg accgactcca ggaacgacag
     3061 ccaggccagg ccacgttctt ggtaggggcg cagcgcaccc gtgaaaccct ccggcgcggt
     3121 catcggcgtg atccggtggt ccacctggcc ggacagcagg tcggcgagcc agccctgcgt
     3181 ctcca
//
LOCUS       DATAIX010000381         1739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_50934, whole genome shotgun sequence.
ACCESSION   DATAIX010000381 DATAIX010000000
VERSION     DATAIX010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50934"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VHM65_05735"
     CDS             complement(<1..386)
                     /locus_tag="VHM65_05735"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2705239.1"
                     /translation="MPILPGAEPYSNDGGDIGVLVCHGYSGSPQSMRPWAEHLAAHGY
                     TVRLPLLPGHGTTWQELNKTRWQDWYGELDTAFRELHERCSQVFVVGLSMGGLLATKV
                     AQEHGPQVSGLVLVNPIYAHPNPLLKL"
     gene            551..1447
                     /locus_tag="VHM65_05740"
     CDS             551..1447
                     /locus_tag="VHM65_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX2705240.1"
                     /translation="MFYWVVKTVIVGPILRLLFRPWVEGDEHIPEAGAAIFASNHLSF
                     SDSIFLPLLLPRRMTFLAKSDYFTGHGFKGRLTAAFFKGIGQLPIDRSGGKAGLAAIN
                     SGLRVLRRGELLGIYPEGTRSPDGRLYRGRTGVARMALEAKVPVLPVAMIGTDKAQPT
                     GKKMPKIMRIGVRIGKPLDFSRYEGMQEDRYVLRAVTDEIMYELMLLSGQEYVDMYAT
                     SMKDRLLKIARVKAREIQEAAKPGRAASELEEALDAAERGDDLGDGLANPPLRIGNAF
                     LDDPVDDPLDGQGHAGPHRRAS"
     gene            complement(1444..1725)
                     /locus_tag="VHM65_05745"
     CDS             complement(1444..1725)
                     /locus_tag="VHM65_05745"
                     /inference="COORDINATES: protein motif:HMM:NF022671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HEX2705241.1"
                     /translation="MPDVPPREPHPCDIAPRHLTVVECRAPWREDYGPQWTRSPIARL
                     RYAKASREWSLYWRDRNERFRTYDLAPPTANVDELLAVLDRDPTRVFWG"
BASE COUNT          268 a          564 c          630 g          277 t
ORIGIN      
        1 agcttgagca gcgggttggg gtgggcgtag atggggttga cgagcacgag accgctgacc
       61 tgggggccgt gctcctgggc gaccttggtg gcgagcaggc cacccatcga caggccgacg
      121 acgaacacct ggctgcagcg ctcgtgcagc tcgcggaagg cggtgtcgag ctcgccatac
      181 cagtcctgcc accgcgtctt gttcagctcc tgccacgtcg tcccgtggcc cggcaggaga
      241 ggcaggcgca cggtgtagcc atgggcggcc aggtgctcag cccagggccg catgctctgc
      301 ggggagccgg agtagccgtg gcagacgagg actccgatgt cgccaccgtc gttcgagtag
      361 ggctctgcac cagggaggat gggcacgtca ggtctccttg tggttgccac gtccgtcgcc
      421 tcatcctcgc agatcgcggg ccgtgcgcgg gatgggccgg cacgggtctg cgggtctggc
      481 cgtggccgat gctctcagtg aactctccgt gaagtagagt gctccggtca cagtccgagg
      541 gagtggtgca gtgttctact gggtggtcaa gacggtcatt gtcgggccga tcctgcggtt
      601 gctcttccgg ccgtgggtcg agggcgacga gcacatcccc gaggcggggg ccgcgatctt
      661 cgcgagcaac cacctgtcct tctccgactc gatcttcctg ccgctgctcc tgccgcggcg
      721 gatgacgttt ctggccaagt ccgactactt caccggtcac gggttcaagg gacgcctcac
      781 ggccgccttc ttcaagggca tcggccagct gccgatcgac cggtcgggtg gcaaggccgg
      841 tctggctgcg atcaactccg gcctgcgggt gctgcgccgt ggtgagctgc tgggcatcta
      901 ccccgagggc acccgctcgc ccgacggccg cctctaccgg gggcgcacgg gcgtggcgcg
      961 gatggccctg gaggccaagg tgccggtgtt gccggtcgcg atgatcggga ccgacaaggc
     1021 ccagccgacc ggcaagaaga tgcccaagat catgcggatc ggcgtgcgca tcggtaagcc
     1081 gctcgacttc tcgcgctacg aggggatgca ggaggaccgc tacgtcctgc gcgccgtgac
     1141 cgacgagatc atgtacgagc tcatgctcct gtcgggccag gagtatgtcg acatgtacgc
     1201 cacgtcgatg aaggaccggc tgctcaagat cgcccgggtc aaggcgcgcg agatccagga
     1261 ggccgccaag ccgggccgcg cggcgagcga gctggaggag gccctcgacg cggccgagcg
     1321 cggcgacgac ctcggtgacg ggctcgccaa ccctccgctg cgcatcggaa acgccttcct
     1381 cgacgacccg gtggacgacc cgctcgacgg ccagggccac gcggggccgc accggcgcgc
     1441 gagctagccc cagaagacgc gcgtcgggtc acggtcgagc accgcgagca gctcgtcgac
     1501 gttggccgtc ggtggtgcga ggtcgtacgt gcgaaaccgc tcgttacggt cacgccagta
     1561 caacgaccac tctctgctcg ccttcgcata gcgaaggcga gcaatcgggg agcgagtcca
     1621 ctgcggacca tagtcctcgc gccagggggc gcggcactcg acgaccgtca ggtgccgcgg
     1681 ggcgatgtca catgggtgcg gctcccgtgg agggacgtca ggcagcggtt gggattgtc
//
LOCUS       DATAIX010000382         4413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_20814, whole genome shotgun sequence.
ACCESSION   DATAIX010000382 DATAIX010000000
VERSION     DATAIX010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20814"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VHM65_05750"
     CDS             complement(<1..255)
                     /locus_tag="VHM65_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705242.1"
                     /translation="MHSLPVCAYVALWATSAYTTGEPAAGLVFQACPGAAFAGDHDRL
                     DLWREAGETVVCAAFPTPGHAHGMPRTSPAALAAVTASGQC"
     gene            306..1139
                     /locus_tag="VHM65_05755"
     CDS             306..1139
                     /locus_tag="VHM65_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2705243.1"
                     /translation="MSDVLAFAGVSVVRAQTRLLDDITWEVEEGERWVVLGPNGAGKT
                     TLLQLASARMHPTTGVAGVLGEVLGTVDVFELRPRIGLASAVIADRIPGGERVIDVVV
                     TASYGIVGRWREDYDALDFGRASELLDALGAAHLADRLFGTLSEGERKRVQIARALMT
                     DPELMLLDEPAAGLDLAGREELVARLGALAADTDAPALILVTHHVEEIPPSFTDVLLL
                     REGRIVAAGPLELTLTAENLSATFGLPLVLERHGDRWAARAESGPRSTVEGTAVSALT
                     S"
     gene            1201..1653
                     /locus_tag="VHM65_05760"
     CDS             1201..1653
                     /locus_tag="VHM65_05760"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX2705244.1"
                     /translation="MEYAWLVWLGVAIALAAVEAATVDFVFVMLAGGALGGAAAAGLG
                     ASFPVQAVVAVVTAVALLAVVRPWAQRTFTVTRDTKIGAAGQIGRSAFVLERVTETDG
                     RVKLAGETWSARSGDGHIYHPGDEVRVASLDGATAVVISAAAPSDPAR"
     gene            1700..2797
                     /locus_tag="VHM65_05765"
     CDS             1700..2797
                     /locus_tag="VHM65_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX2705245.1"
                     /translation="MEPTLIVIVLLIVFAVIVLVRTIRIVPQQTAQIVERLGSYSRTL
                     DDGMHILVPFVDKVRANIDLREQVVSFPPQPVITSDNLVVSIDTVIYYSVNDAKSAVY
                     EIANFIQGIEQLTVTTLRNVIGSLDLEQTLTSRDQINAQLRGVLDEATGKWGIRVNRV
                     ELKAIDPPHSVQDSMEKQMRAERDRRATILTAEGVKQAAILTAEGDKQSQILRAEGSA
                     QARILESQGQARAIQQVFDAIHRGKPTQKLLAYQYLQVLPQIAHGGANKMWIIPSELT
                     DALRGITGALGGVAKTGEDPDDDAWVDPGDVPTVFAETILEDPAKALAEARGQAGQAS
                     AEATEHASPMARRQPPVQPPADRVPPAGDQT"
     gene            2865..3632
                     /locus_tag="VHM65_05770"
     CDS             2865..3632
                     /locus_tag="VHM65_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018104742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX2705246.1"
                     /translation="MPWFEPVAILLAGVGAGTINTIVGSGTLITFPTLLFFGYPPILA
                     NVSNTLGLVAGGASGTWGYRRELAGHGEAVRRLLPLSLAGSVLGSALLLVLPAKAFAT
                     IVPVLIVLALCLVVFGPRIQRAAAHRHTDASGGWRTPALLGGTFFAGVYGGYFGAAQG
                     VLLVGLFSLVTSYPLQRANAYKNILATAVNLVAAAVFLLFARDQIDWVVAARVAVGAT
                     AGGVIGAHVGRRLPPQVLRVVIVTVGVLAIVKMVAFP"
     gene            3632..>4413
                     /locus_tag="VHM65_05775"
     CDS             3632..>4413
                     /locus_tag="VHM65_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX2705247.1"
                     /translation="MPELIEDPADERLADFVALTDVALRRRIEPERGLYIAESEKVIR
                     RALAAGHRPRSLLMTSRWLVGLADVVMHAEASGVPVYVGGHAVIEALTGFHLHRGALA
                     AMHRPALPAVEDLLAGAGRVVVLEDIVDHTNVGAVTRSCAALGVDAMFVTPRCADPLY
                     RRAIRVSMGTVFQVPWTRLGPWPEGVERLRAGGFTVAALALGDDAISLDAVAADPPTR
                     LALVLGTEGDGLSRHTVAAADLAVRIPMGGGVDSLNVAAASAV"
BASE COUNT          595 a         1589 c         1567 g          662 t
ORIGIN      
        1 gcactgaccg ctcgccgtca ccgcggccag cgcggccggt gaggtgcgcg gcataccgtg
       61 ggcatgtccg ggggtgggga aggcggcaca gacgacggtc tcgccggcct cgcgccacag
      121 gtcgagccgg tcgtggtcgc cggcgaaggc ggcaccaggg caggcctgga agacaagccc
      181 ggccgcgggc tcgcccgtgg tgtaggcgga cgtcgcccac agggcgacgt aggcgcagac
      241 gggcagcgag tgcaccggcc aactctagga cggctcatct agggtgaagg catgagggtg
      301 gaccggtgag cgacgttctc gccttcgcgg gggtcagcgt cgtgcgagcg cagaccaggc
      361 tcctggacga catcacctgg gaggtcgagg agggcgagcg ctgggtcgtc ctcggcccca
      421 acggggcggg caagacgacg ctgctgcagc tggcctcggc gcggatgcac ccgaccaccg
      481 gtgtcgcggg ggtgctcggt gaggtcctcg ggaccgtcga cgtcttcgag ctgcggcccc
      541 ggatcggctt ggccagcgcg gtgattgccg accggatccc cggcggcgag cgagtgatcg
      601 acgtcgtcgt caccgcgtcc tacggcatcg tggggcggtg gcgggaggac tacgacgcgc
      661 tggacttcgg gcgcgcgagc gagctgctcg acgcgctcgg cgccgcccat ctggccgacc
      721 ggctcttcgg cacgctgagc gagggcgagc gcaagcgggt ccagatcgcc cgggcgctca
      781 tgaccgaccc cgagctgatg ctgctcgacg agccggccgc cggtctggac ctcgccgggc
      841 gcgaggagct tgtcgcccgg ctcggggccc tcgccgcaga caccgacgcc cccgccctga
      901 tccttgtcac ccatcacgtc gaggagatcc cgccgtcgtt caccgacgtg ctcctgctgc
      961 gcgaggggcg catcgtcgcg gcgggaccgc ttgagctgac actcaccgcc gagaacctct
     1021 cggcgacgtt cggcctccca ctcgtcctcg agcggcacgg ggaccgctgg gctgcccgcg
     1081 ccgagtcggg gccaaggtct acggtggagg gaaccgcggt ttccgcgttg acgtcgtagc
     1141 agtacggatc ggtgaccacg gcctcgcacc tgggggccgg gcgccggaga ggacaccgcc
     1201 gtggagtacg cctggttggt ctggctgggt gtggccatcg ccctggctgc agtcgaggcc
     1261 gccacggtcg acttcgtctt cgtcatgctc gccggaggag cactgggcgg tgccgccgca
     1321 gccgggctcg gtgcctcctt cccggtccag gcggtggtcg cggtcgtcac cgcggtggcc
     1381 ctgctcgccg tcgtgcgccc ctgggcccag cgcacgttca ccgtcacgcg ggacaccaag
     1441 atcggggcgg ccgggcagat cgggcgctcg gcgttcgtcc tcgagcgggt gaccgagacc
     1501 gatggccggg tcaagctcgc cggagagacc tggtcggccc ggtccggaga cggtcacatc
     1561 tatcatcccg gcgacgaggt gcgcgtcgcc tcgctcgacg gcgcgaccgc cgtcgtcatc
     1621 tcagcggccg caccgtcgga ccctgcgcgc tagcccgccc gctcgtcacc caccgacatc
     1681 gccaagaacg gagccagccg tggaaccgac cctcatcgtc attgtcctgc tcatcgtctt
     1741 cgccgtgatc gtgctcgtcc gcacgatccg gatcgtgccg cagcagaccg cgcagatcgt
     1801 cgagcggctc ggcagctact cgcgcaccct cgacgacggc atgcacatcc tcgtgccgtt
     1861 cgtcgacaag gtgcgggcca acatcgacct gcgtgagcag gtcgtcagct tcccgccgca
     1921 gccggtcatc acctcggaca acctcgtcgt gagcatcgac accgtcatct actactcggt
     1981 caacgacgcg aagtcggccg tctacgagat cgccaacttc atccagggca tcgagcagct
     2041 taccgtcacc accttgcgca acgtcatcgg ctcgctcgac ctcgagcaga ccctgacctc
     2101 gcgcgaccag atcaacgccc agctgcgcgg ggtcctcgac gaggcgaccg gcaagtgggg
     2161 catccgggtc aaccgggtcg agctcaaggc gatcgacccc ccgcactcgg tccaggactc
     2221 gatggagaag cagatgcgcg ccgagcgtga ccggcgggcg acgatcctca ccgccgaggg
     2281 cgtcaagcag gccgccatcc tcactgccga gggcgacaag cagtcgcaga tcctgcgcgc
     2341 ggagggctcg gcccaggctc gcatcctgga gtcccagggc caggcccgcg cgatccagca
     2401 ggtcttcgac gcgatccacc ggggcaagcc gacgcagaag ctgctcgcct accagtacct
     2461 ccaggtgctg ccgcagatcg cccatggcgg tgccaacaag atgtggatca tcccctcgga
     2521 gctcaccgac gcactgcgcg gcatcactgg ggcactgggc ggcgtggcca agacgggcga
     2581 ggacccggac gacgatgcgt gggtcgaccc cggcgacgtg ccgacggtct ttgccgagac
     2641 gattctcgag gaccccgcca aggccctcgc cgaggctcgt ggtcaggccg ggcaggccag
     2701 cgccgaggcg accgagcacg catcaccgat ggcgcgccga cagccgcccg tccagccacc
     2761 ggccgaccgg gtccccccag cgggcgacca gacctgaacg cccgctgtgc cagcgctctg
     2821 gtacgacgca cccggcaccc gggcaccctg actagggtgg tcgggtgcca tggttcgagc
     2881 cggtcgcgat cttgctggcg ggcgtcggcg caggcacgat caacaccatc gtcggttcgg
     2941 gcacgctcat caccttcccg acgctgctct tcttcgggta cccacccatc ctcgccaacg
     3001 tctcgaacac cctcggcctg gtcgccggcg gcgcgtcggg gacgtggggc taccgccgcg
     3061 agctggccgg ccacggggag gccgtccggc gcctgctgcc gctcagcctg gccgggtcgg
     3121 tcctggggtc cgcgctgctc ctggtgctgc ccgccaaggc cttcgcgacg atcgtccccg
     3181 tcctcatcgt cctggccctg tgcctggtcg tgttcgggcc gcggatccag cgggcggcgg
     3241 cgcaccgcca taccgacgcc tctggcgggt ggcggacgcc tgcactgctc gggggcacct
     3301 tcttcgcagg tgtgtacggc gggtacttcg gtgccgcgca gggcgtcctg ctcgtcgggc
     3361 tcttctcgct cgtgacgagc taccccttgc agcgcgcgaa cgcctacaag aacatcctcg
     3421 cgacagcggt caacctcgtg gcggcagcgg tgtttctcct cttcgcccgg gatcagatcg
     3481 actgggtcgt ggcggcgcgc gtcgcggtgg gcgcaaccgc cggaggggtc atcggcgctc
     3541 atgtcggccg gcggctgccg cctcaggtgc tgcgtgtggt catcgtcacg gtgggggtcc
     3601 tcgcgatcgt caagatggtg gcttttccct gatgccggag ctcatcgagg acccggcgga
     3661 cgagcggctt gccgacttcg tggcgctcac cgacgtcgcg ctgcgccgac ggatcgagcc
     3721 cgagcgcggc ctctacatcg ccgagagtga gaaggtgatc cgccgggctc tcgcggcggg
     3781 gcaccggccg aggtcgctgc tcatgacctc gcgctggctc gtcggcctcg ccgacgtcgt
     3841 catgcacgcg gaggcgtccg gcgtccccgt gtatgtcggt gggcacgcgg tcatcgaggc
     3901 gctcaccggc ttccacctgc atcggggggc gctggcggcg atgcaccggc cggccctgcc
     3961 tgcggtcgag gacctcctgg ccggcgcggg gcgggtcgtc gtgctcgagg acatcgtcga
     4021 ccacaccaac gtcggggcgg tgacccgcag ctgcgccgcg ctcggcgtcg acgccatgtt
     4081 cgtcacgcct cggtgtgccg acccgctcta ccggcgggcg atccgagtct cgatggggac
     4141 ggtcttccag gttccgtgga cacggctcgg gccctggccg gagggggtcg agcggctgcg
     4201 cgcgggcggg ttcaccgtgg ccgcgctcgc cctgggcgac gacgccatct cgctggacgc
     4261 cgtcgcggcc gacccgccga cgcgcctggc cctcgtcctc ggcaccgagg gcgacgggct
     4321 gtcccgtcat accgtcgctg ccgcggatct cgccgtgcgg atccccatgg gcggcggcgt
     4381 ggactcgctc aacgtggccg ctgccagtgc cgt
//
LOCUS       DATAIX010000383         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_170355, whole genome shotgun sequence.
ACCESSION   DATAIX010000383 DATAIX010000000
VERSION     DATAIX010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170355"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..789)
                     /locus_tag="VHM65_05780"
     CDS             complement(46..789)
                     /locus_tag="VHM65_05780"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX2705248.1"
                     /translation="MSPRSEDRARRPVIGITTYVELASWGLWRDAPAALVPHGYVAHV
                     ESAGGVALLVPPRVGASAAVVAAVLGSLDGLVIAGGADVDPARYGADRHPSVEPSRAD
                     RDSMELALVRGAVARDLPLLGICRGMQVMAIAAGGSLIQHVPDVTGSAAHSPALATFG
                     STQVSTVDGSLVAGIVGARMEVPCCHHQAVASAPGYVASASASDGVMEAMEAPDARFR
                     MAVQWHPEASTETALMRALILAAVPSRRS"
     gene            complement(773..1540)
                     /locus_tag="VHM65_05785"
     CDS             complement(773..1540)
                     /locus_tag="VHM65_05785"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEX2705249.1"
                     /translation="MSGRLDAVVAVVTGGCSGIGLATVRRFADEGATVVIGDLDDAVG
                     EQIAAEVGGAYVHCDVTDAEQVEALFRTAKERFGSVDVAFNNAGISPPEDDSILTTGI
                     ETWRRVQEVNLTSVYLCCKAALPYMLEQGKGSIINTASFVARLGAATSQISYTASKGG
                     VLAMSRELGVQFARQGVRVNALCPGPVNTPLLQELFANDPERAASRLVHIPMGRFGEA
                     EEIANAAVFLASDESSFITACDFLVDGGISGAYVTPL"
     gene            complement(1600..>2445)
                     /locus_tag="VHM65_05790"
     CDS             complement(1600..>2445)
                     /locus_tag="VHM65_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2705250.1"
                     /translation="VPEGVLTVLAGQGSVVGERFVTHPDVRKVCFTGSTAVGRQIMRG
                     AAEQVKRVTLELGGKSANIVFDDADIEAAAATAPYAVFDNAGQDCCARSRILVQRTVF
                     ERFMELFEPAVKGVKVKDPGAEDAEMGPLISRSHHDAVASFVEGADEPVDVAFRGSAP
                     AGPGYLYAPAVVLPRSARDRVWREEVFGPVVAVLPFDDEADAISKANDSPYGLSGSIW
                     TRDVGRAMRVARGVECGNLSVNSHSSVRYSTPFGGFKQSGLGRELGPDALDSFTEVKN
                     VFFAL"
BASE COUNT          381 a          888 c          855 g          321 t
ORIGIN      
        1 gctcccagat gagccgcggg cccggtgcgg gcagctcgag aggcttcaac tgcgccggct
       61 cggcacggcg gcgaggatca gggcgcgcat cagggcggtc tcggtgctcg cctcggggtg
      121 ccactggacc gccatccgga accgggcatc cggcgcctcc atcgcctcca tgactccatc
      181 ggaggcggat gccgacgcga catatccggg cgccgaggcc acggcctggt ggtggcagca
      241 cggcacctcc atccgcgccc cgacgatccc cgcgaccagc gagccgtcaa ccgtcgagac
      301 ctgggtcgag ccgaatgtcg cgagcgcagg cgagtgcgcg gccgaccccg tcacgtcggg
      361 cacgtgctgg atgagcgacc ccccggcggc aatcgccatg acctgcatcc cccggcagat
      421 cccgagcagg ggcaggtccc gcgcgacggc cccgcggacg agcgccagct ccatggagtc
      481 gcggtcagca cgggaaggct ccaccgaggg gtgacggtct gcgccatacc gcgccgggtc
      541 gacgtcggct cccccggcga tgacgaggcc gtcgagcgag cccagcacgg ccgcgaccac
      601 cgccgcggag gcgccaaccc gcgggggcac gagcagcgcc acgccccccg cggactcgac
      661 atgtgcgaca tacccgtgcg ggacgagcgc ggcgggggcg tcccgccaca gtccccacga
      721 ggcgagctcg acatacgtcg tgatcccgat gacggggcgg cgcgcgcggt cctcagagcg
      781 gggtgacata ggccccggag atcccgccgt cgacgaggaa gtcgcacgcg gtgatgaagc
      841 tcgactcgtc cgaggcgagg aagaccgcgg cgttggcgat ctcctcggcc tcgccgaagc
      901 ggcccatcgg gatgtgcacc aggcggcttg cggcacgctc ggggtcgttg gcaaagagct
      961 cctgcagcag cggcgtgttg accggccccg ggcacagcgc gttgacccgc accccctgac
     1021 gagcgaactg cacgccgagc tcgcgcgaca tcgcgagcac gccgcccttg gaggcggtgt
     1081 aggagatctg cgaggtcgcc gcgcccaggc gggccacgaa cgacgccgtg ttgatgatcg
     1141 agcccttgcc ctgctcgagc atgtagggca gggccgcctt gcagcacaga tagaccgagg
     1201 tgaggttgac ctcctgcact cgccgccacg tctcgatccc tgtggtgagg atcgagtcgt
     1261 cctcgggtgg ggagatgccg gcgttgttga acgcgacgtc gaccgagccg aacctctcct
     1321 tggccgtgcg gaagagcgcc tcgacctgct cggcgtccgt cacgtcgcag tggacgtagg
     1381 cgcccccgac ctcggccgcg atctgctcgc ccaccgcgtc gtcgaggtcg ccgatgacga
     1441 ccgtggcgcc ctcgtcggcg aaccggcgca ccgtcgcgag cccgatcccg ctgcagcccc
     1501 cggtgaccac ggcaaccacg gcgtcgaggc gaccagacat gtgcggtgct cctgttctcg
     1561 tgcggtcccg tgccgcgccg gtgcggatga cgtgcgcggc tagagtgcga aaaagacgtt
     1621 cttgacctcg gtgaaggagt cgagggcgtc cgggccgagc tcgcgcccga gcccggactg
     1681 cttgaacccg ccgaatggcg tcgagtaacg caccgacgag tgcgagttca ccgacaggtt
     1741 cccgcactcg acaccccggg ccacccgcat cgctcgcccg acgtcgcggg tccagatcga
     1801 ccccgacagc ccgtatggcg agtcgttggc cttgctgatg gcgtcggctt cgtcgtcgaa
     1861 gggcaggacg gcgacgaccg gcccgaagac ctcctcacgc catacccggt cgcgcgccga
     1921 acggggcagc accaccgcgg gtgcgtacaa gtagccgggg ccggccggcg ccgagccgcg
     1981 gaaggcgacg tcgaccggct cgtccgcgcc ctcgacgaac gaggccacgg cgtcgtggtg
     2041 gctacgactg atgagcggac ccatctcggc gtcctcggcc cccgggtcct tgaccttgac
     2101 ccccttgacg gccggctcga atagctccat gaaacgctcg aagacggtgc gctgcaccag
     2161 gatccggcta cgggcgcagc agtcctggcc cgcattgtcg aagacagcgt aaggcgcggt
     2221 cgccgcggcc gcctcgatgt cggcgtcgtc gaagacgatg ttggcgctct tgccaccgag
     2281 ctccaaggtg acccgcttga cctgctcggc cgccccgcgc atgatctgcc gcccgaccgc
     2341 cgtcgagccg gtgaagcaca ccttgcgcac gtcgggatgc gtgacgaagc gctcccccac
     2401 gaccgagccc tggccggcca ggaccgtgag gacgccctcg gggac
//
LOCUS       DATAIX010000384         1596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_277356, whole genome shotgun sequence.
ACCESSION   DATAIX010000384 DATAIX010000000
VERSION     DATAIX010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277356"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VHM65_05795"
     CDS             <1..633
                     /locus_tag="VHM65_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705251.1"
                     /translation="TSMTVEVRYDDAAAGYARRFTQVLTPVPAAAPVPTEPAPPTGPV
                     APAGQTPTTPAPPAASPGSRWAPVTMSWPILDAARQEIQYRVTTAAGGVIDAGEWTPT
                     TDPSILVGDLGRRSRTVEVRLIGPPLAEANLDAIQVRVGVAGTADADAQSVFFDASAG
                     VAQSLTLPAAPGAPPGFRFQSTAFRTDGTQHVTDWASTAQPLIVISTRIV"
     gene            652..>1596
                     /locus_tag="VHM65_05800"
     CDS             652..>1596
                     /locus_tag="VHM65_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705252.1"
                     /translation="MLDLQSRMEILGITTYRDADDPTQYYYVHGTPRISRDAGGPMFD
                     LFTYRKGGEAGSTRAGGFLTMTVDCGLGDLFEAIKAKLTARAGSEVTLSSVPYRSGTV
                     RLIALDETAAGASPIPGVAAGPRFVEQVLGAGSPSLDGDNRAIFSLTLSEDGVAFFLG
                     VLQGTANARPLGVVYNLEYVGLLPAYGVEIAIDMKSCYDFMETRFTAGTLFFKADIDS
                     ITEKLKSENHISIKEKVRVLELSTPEAIAARTKSLNDLVTTLASGALFQPTLAPGQPK
                     VAGNLMTATMPTGVPPGTTPPVTTPPGTTPPVTTPPGTT"
BASE COUNT          255 a          622 c          507 g          212 t
ORIGIN      
        1 acctcgatga ccgtcgaggt ccggtatgac gacgccgccg ccggctatgc gcgccgcttc
       61 acgcaggtgc ttacgcccgt gcccgcggcg gcaccggtgc ccaccgagcc tgcgccgccg
      121 accggcccgg tggcgcccgc gggccagaca ccgacaaccc cagcgccacc cgccgcctcc
      181 cccgggagcc gatgggcgcc ggtgacgatg agctggccga tcctcgacgc cgctcgccag
      241 gagatccagt accgcgtgac gaccgccgcc ggtggcgtca tcgacgccgg ggagtggact
      301 cccacgaccg acccgtcgat cctcgtcggc gacctcgggc ggcgcagccg gacggtcgag
      361 gtccggctca tcggaccacc gctcgcagag gcgaacctcg acgccatcca ggtgcgcgtc
      421 ggcgtggccg ggaccgccga cgcagacgcc cagagcgtgt tcttcgacgc gagtgcgggc
      481 gtcgcccaga gcctcaccct ccctgcagcc ccgggcgcgc cgccggggtt ccgcttccag
      541 agcacggcgt tccgcaccga tggcacccag cacgtcaccg actgggcgag cacggcccag
      601 cccctcatcg tcatctcgac ccgcatcgtc tgagcacgga aaggaccggc catgctcgac
      661 ctgcagagcc gcatggagat tctcggcatc acgacctacc gggatgccga cgaccccacg
      721 cagtactact acgtccacgg caccccgcgt atcagccgcg acgccggcgg tccgatgttc
      781 gacctgttca cctaccgcaa gggtggcgag gcggggtcga cccgggccgg tggcttcctc
      841 accatgacgg tcgactgcgg cctgggcgac ctcttcgagg ccatcaaggc caagctgacc
      901 gcccgggccg gctcggaggt cacgctgtcg agcgtgccct accgctccgg gacggtgcgc
      961 ctcatcgcgt tggacgagac cgctgcgggc gcctccccca tccctggagt cgcggccggg
     1021 ccccggttcg tcgaacaggt cctcggagcc ggctcgccct ccctcgacgg cgacaaccgg
     1081 gcgatcttct cgctgaccct gagcgaggac ggtgtcgcgt tcttcctcgg agtcctccag
     1141 ggcacggcca acgcgcgacc gctcggggtg gtctacaacc tggagtacgt cgggctgctg
     1201 ccggcctacg gagtggagat cgccatcgac atgaagagct gctacgactt catggagacc
     1261 cggttcaccg ccgggacgct cttcttcaag gccgacatcg acagcatcac cgagaagctc
     1321 aagtccgaga accacatctc gatcaaggag aaggtccggg tcctcgagct ctcgacgccc
     1381 gaggcgatcg ccgcccggac caagtcgctc aacgacctcg tgacgaccct ggcaagtggc
     1441 gctctcttcc agccgacgct cgcgcccggg cagcccaagg tcgccggcaa cctcatgacg
     1501 gccaccatgc ccacgggtgt gccgccgggc acgacacccc cagtcaccac gccgccgggc
     1561 acgacacccc cagtcaccac gccgccgggc acgaca
//
LOCUS       DATAIX010000385         1706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_247536, whole genome shotgun sequence.
ACCESSION   DATAIX010000385 DATAIX010000000
VERSION     DATAIX010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247536"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VHM65_05805"
     CDS             <1..405
                     /locus_tag="VHM65_05805"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2705253.1"
                     /translation="GAYVMAEASGGAPDVILVATGSEVAIAVVARHELEARKIGTRVV
                     SMPCREWFDRQPEAYRDSVLPPSIRARVSVEAGVGQGWRDIVGDAGQIVSIEHFGASA
                     DGATLFREFGFTPAAVVTAARASIIAAKAGAR"
     gene            402..1514
                     /gene="tal"
                     /locus_tag="VHM65_05810"
     CDS             402..1514
                     /gene="tal"
                     /locus_tag="VHM65_05810"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX2705254.1"
                     /translation="MTARLADLTGAGVSIWLDDLSRERLRTGSLQAVVDTKYVTGVTT
                     NPSIFAAALAQGDAYRDQVAELAAAGADGDAAAFALTTSDVRDACDVLRPVYDRTGGV
                     DGRVSIEVDPRLARQTAPTIEAARVLHDAVGRPNVMVKIPATVEGLPAITATLAAGIS
                     VNVTLIFSLQRYRDVMNAFLRGLEQANEMGRDLSTIHSVASFFVSRIDTEIDKRLDAI
                     GSTAAVALKGQAGIANARLAYHAYEEVFSTPRWRSLADEGAHAQRPLWASTGVKDPAY
                     PDTMYVTELVAAGVVNTMPEKTLDATADHGPVRGDTVRHFYDGARLVFDGLDGVGVSY
                     TDVVGVLEGEGVEKFEKSWADLLAGVSAGLAAVGAP"
     gene            1511..>1706
                     /locus_tag="VHM65_05815"
     CDS             1511..>1706
                     /locus_tag="VHM65_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009994655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705255.1"
                     /translation="MSPSRIAPGRNPLRDPRDKRLPRIAGPCGMVMFGVTGDLARKKL
                     MPAIYDLAGRGLLPPGFSLVG"
BASE COUNT          236 a          615 c          603 g          252 t
ORIGIN      
        1 ggtgcctatg tcatggccga ggcgagcggc ggcgctcccg acgtcatcct cgtggcgacg
       61 ggctccgagg tcgcgatcgc cgtcgtggct cggcacgagc tcgaggccag gaagatcggc
      121 acccgagtcg tctcgatgcc ctgccgggag tggttcgacc gccagcccga ggcctaccga
      181 gactcggtgc tcccaccgag catcagggcg cgggtcagcg tcgaggccgg agtcggccag
      241 ggctggcgcg acatcgtcgg cgacgccggc cagatcgtga gcatcgagca cttcggcgcc
      301 tcggccgacg gggccacgct gttccgggag ttcggtttca cacccgcggc agttgtcacc
      361 gcggcccggg cctcgatcat tgccgcgaag gcgggtgccc gatgacggcg cgtctcgccg
      421 acctgaccgg cgcaggagtc tcgatctggc tcgatgacct gtcccgtgag cggctgcgga
      481 cgggcagcct gcaggcggtg gtcgacacca agtacgtgac gggtgtgacg acgaacccct
      541 cgatcttcgc cgcggcgctc gcccagggcg acgcctaccg cgaccaggtc gccgagctgg
      601 cagccgccgg ggccgacggt gacgcggcgg ccttcgcgct gaccacgtcg gacgtccggg
      661 acgcctgtga cgtccttcgc ccggtctacg accgcacggg cggggtcgac ggccgggtgt
      721 cgatcgaggt cgacccccgg ctggcccggc agacggcgcc gacgatcgag gcggcccgcg
      781 tccttcatga cgcggtgggc cgcccgaacg tcatggtcaa gatcccggcc acggtcgagg
      841 ggctgcccgc catcaccgcg accctggcgg ccgggatcag cgtcaacgtg acgctgatct
      901 tctccctgca gcgctaccgc gacgtgatga acgccttcct gcgcggcctc gagcaggcca
      961 acgagatggg tcgcgacctg tcgacgatcc actcggttgc gtcgttcttc gtctcgcgca
     1021 tcgacaccga gatcgacaag cggctcgacg cgatcggttc cacggccgcc gtcgcgctca
     1081 agggtcaggc cggcatcgcc aacgcgcggc tggcctacca cgcctacgag gaggtcttct
     1141 cgaccccccg gtggcgatcc ctggccgacg agggcgccca tgcgcagcgg cccctgtggg
     1201 cctcgaccgg cgtcaaggac ccggcctacc cggacacgat gtatgtcacc gagctcgtcg
     1261 ctgcgggggt ggtcaacacc atgccggaga agaccctcga cgcgacggcc gaccatggtc
     1321 ccgtgcgcgg ggacaccgtc cgccacttct atgacggcgc ccggctcgtc ttcgacggcc
     1381 tcgacggcgt cggggtctcc tacaccgacg tggtcggcgt gctcgaaggt gagggcgtcg
     1441 agaagttcga gaagtcctgg gccgacctgc tcgccggcgt cagtgccggg cttgccgccg
     1501 tgggggcgcc atgagcccat cccgcatcgc acccgggcgc aacccgctgc gcgacccgcg
     1561 ggacaagcga cttccgcgga tcgcgggccc ctgcgggatg gtgatgttcg gcgtcaccgg
     1621 cgacctcgcg cgcaagaagc tcatgcccgc catctacgac ctggccgggc gggggctgct
     1681 gccgcccggt ttctccctcg tcgggt
//
LOCUS       DATAIX010000386         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_277379, whole genome shotgun sequence.
ACCESSION   DATAIX010000386 DATAIX010000000
VERSION     DATAIX010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277379"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1345)
                     /locus_tag="VHM65_05820"
     CDS             complement(173..1345)
                     /locus_tag="VHM65_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2705256.1"
                     /translation="MEERRRAMVEAVRAGASRRAVARRFGVSLPTVQRWVARAGTGAL
                     DAVDWGDRSSAPRRQARRASPAAEGLVRRLRIELAGSVLGEHGALAIRLALETRSDAP
                     RQLPSVRTIGRILERGGLLDGRRRVRHPPPPRGWYLPDVREHRQELDQMDSIEELRLV
                     GGIPLEVLTLISLHGALPAAWPGPRLVTDRVIGSLLEHWRAHGLPGYAQFDNALVFAG
                     THARPDLGRVARTCLSLGVTPVYAPPRETGFQAAIEAFNGRWRRACWERPDTLTMAST
                     RDRSTAWLEAWRTARAVRIEAAPPRSPFPADFDPEAPPVHRGRVVYLRRTDGKGFATV
                     LTRRFEVAADWPHRLVRAEVDLDIGAIRFHALRRQQPDDHPLLRTVPFQAPWPPLR"
BASE COUNT          206 a          564 c          552 g          191 t
ORIGIN      
        1 cgtctggggc cgggccgcca gcgtgacgat cctccacgat cagaggcaca accgtcgggc
       61 tgagcatcgt gaccagcctc agatggtgtc gatcggggct ctgaggatgc cccaaccccg
      121 catccgcgac ccgactcgtt ctctgatggg gccaggtgtc atcacctcgg tgtcaccgca
      181 ggggtggcca gggtgcctgg aagggcaccg tccggagcaa cggatggtcg tctggctgct
      241 gtcgcctgag tgcatggaac ctgatcgcgc cgatgtcgag gtcgacctcc gcccggacca
      301 gccggtgcgg ccagtcagcc gccacctcga accgccgggt caggacggtc gcgaagccct
      361 tgccgtcggt ccgccgcagg tagaccaccc gcccgcggtg gacgggtggt gcctccgggt
      421 cgaagtccgc agggaacggt gaccggggcg gagcggcctc gatgcgcacc gcacgggccg
      481 tccgccaggc ctccagccag gcggtcgagc ggtcccgggt cgaggccatc gtcagtgtgt
      541 ccgggcgctc ccagcaggcg cgcctccagc ggccgttgaa ggcctcgatg gccgcctgga
      601 agcccgtctc ccggggtggc gcgtagaccg gcgtgacacc cagcgagagg caggtccgcg
      661 cgacccgccc gaggtccggc cgtgcatggg tgcccgcgaa caccagcgcg ttgtcgaact
      721 gggcgtagcc gggcagtccg tgggcgcgcc agtgctcgag gagcgacccg atgacccggt
      781 cggtgaccag ccggggaccc ggccacgcgg cgggcagggc gccgtggagc gagatgagcg
      841 tgagcacctc gagcgggatg ccgcccacca ggcgcagctc ctcgatggag tccatctggt
      901 cgagctcctg ccggtgctcc cggacgtccg gcaggtacca gccgcgcggg ggcggtgggt
      961 ggcgcacccg gcggcggccg tcgagcaggc cgccccgctc caggatgcgc ccgatggtgc
     1021 gcaccgatgg cagctgccgg ggtgcgtcgg acctcgtctc gagcgcgagc cggatggcga
     1081 gcgcgccatg ctccccgagg acgctcccgg ccagttcgat ccggaggcgc cggaccagcc
     1141 cctcggcggc cgggctggcg cgccgggcct ggcgccgagg tgccgacgac cggtcccccc
     1201 agtccaccgc gtcgagcgcg ccggtcccgg cacgcgccac ccagcgctgg acggtcggca
     1261 gggacacccc gaagcgccgc gccaccgccc gccgggaggc acccgcccgg accgcctcga
     1321 ccatcgcccg gcgccgctcc tcgatgagcc gctgggcctg gcctttttgg tcacgcgacc
     1381 accctccgac ctaccgtttt gggcgcagcc taccgacacc gaggtgatgg cacgtggccc
     1441 gatcagcgaa cgcgcacgac ggtgtcggcc ggttcacggc ccgtgacacg cctccaggcc
     1501 cacacttctg cac
//
LOCUS       DATAIX010000387         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_81147, whole genome shotgun sequence.
ACCESSION   DATAIX010000387 DATAIX010000000
VERSION     DATAIX010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81147"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VHM65_05825"
     CDS             <1..285
                     /locus_tag="VHM65_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705257.1"
                     /translation="SRTSAALMPSPPQPVVLTAAEVREQVLSGLADEARRMLDEGVVQ
                     APMDLDLAMITGAGFAFSNGGLTPLLDRSGVAERVTGRRFLPPGVASVPA"
     gene            371..1900
                     /locus_tag="VHM65_05830"
     CDS             371..1900
                     /locus_tag="VHM65_05830"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX2705258.1"
                     /translation="MIENYLSRIGGLIRDARRHKGLTQHELAELLGTSQSAVARIEQG
                     KQNLSLEMLARIGEKLDSEFVSLGRPGPQHLRIVGGTELHGSIDVKTSKNAAVALLCA
                     SLLNRGKTTLRRLARIEEVNRIIEVLTSIGVKTRWLPDSSDLEITPPAELSLDAIDVE
                     AARRTRTVLMFLGPLLHSAREFSLPYAGGCDLGTRTVEPHMSALRAFGLHVAATHGFY
                     RATVDPVVGPARPIILTERGDTVTENVLLAAARCEGETLIRNASPNYMVQDLCFFLQE
                     LGVQIEGIGTTTLRVRGRRVIEVDVEYWPSEDPIEAMSLLAAAVVTNSTITICRVPIE
                     FLEIELATLEGMGMRYEMTPEYPSANGRTRLVDLTTVPGPLKAPGDKIHPMPFPGLNI
                     DNLPFFALIAASAVGTTMLHDWVYENRAIYLTELTKVGAKVQLLDPHRVLIEGPTRWR
                     AAEVICPPALRPAVVVLLAMLAAPGTSVLRNVYVINRGYEELAERLNALGATIEVFRD
                     I"
     gene            complement(1936..>2462)
                     /locus_tag="VHM65_05835"
     CDS             complement(1936..>2462)
                     /locus_tag="VHM65_05835"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2705259.1"
                     /translation="VQVRRALREAVAISGAPSVIRFSKGNPPAPIKAVRVTGQVDILR
                     DPDPDGNGVDVLVVGLGAMASVALTAAAKLEAQGFAVRVVDPRWALPVSAGLVDLARD
                     ARAVAVIEDNLVIGGVGSQIAQALRDGGLDVPVHLYGIPKAFLGHGSRGEVLEEAGLT
                     PEAVADRLSEALRA"
BASE COUNT          378 a          914 c          811 g          359 t
ORIGIN      
        1 tcacggacct cggccgcgct gatgccgtcg ccaccacagc cggtggtcct gaccgcggcc
       61 gaggtccgtg aacaggtcct gtccgggctg gccgacgagg cccgtcgcat gctcgacgag
      121 ggcgtcgtcc aggcgccgat ggacctcgac ctcgcgatga tcaccggtgc cggcttcgcc
      181 ttcagcaatg gtggtctgac gccactgctc gaccgttccg gcgtcgccga gcgggtcacc
      241 ggcaggcgct tccttccccc gggcgtagcc agcgtcccgg cctgacccgg gccgggagag
      301 ggtgctgccc gcggtggctg gcgctgcgtc ataccctgtc ctatctcata tatgacgtag
      361 gatcgtgggc atgattgaga actatctctc ccggatcggg gggctgatcc gggacgctcg
      421 ccgccacaag gggttgaccc agcacgagct cgccgagctc ctgggcacga gccagagcgc
      481 ggtcgcccgg atcgagcagg gcaagcagaa cctcagcctg gagatgctgg cccggatcgg
      541 ggagaagctc gactccgagt tcgtctcgct cggccgcccc gggccgcagc acctgcgcat
      601 cgtcgggggc accgagctgc acgggtcgat cgacgtcaag acctcgaaga acgccgccgt
      661 tgcgctgctc tgcgcctcgc tgctcaaccg cggcaagacc acgctgcgcc gcctcgcgcg
      721 catcgaggag gtcaaccgca tcatcgaggt cctcacctcg atcggggtca agacccgctg
      781 gctccctgac agcagcgacc tggagatcac ccctccggca gaactcagtc tcgacgcgat
      841 cgacgtcgag gcggcgcgac gcacgcgcac cgtcctcatg ttcctcggtc cgctgctgca
      901 cagcgcgcgg gagttctccc tgccgtacgc cggcggctgc gacctgggca cccgcaccgt
      961 tgagccgcac atgagcgccc tgcgagcctt cgggctgcat gtcgcggcca cccacgggtt
     1021 ctaccgggcc accgtcgacc cagtggtggg gccggcgcgc ccgatcatcc tcaccgagcg
     1081 tggcgacacc gtgaccgaga acgtcctgct tgccgccgcg cggtgcgagg gggagaccct
     1141 catccgcaac gcgagcccca actacatggt ccaggacctg tgcttcttcc tgcaggaact
     1201 cggcgtgcag atcgagggca tcggcacgac caccctgcga gtccgcggac gccgggtcat
     1261 cgaggtcgac gtcgagtact ggccgtccga ggaccccatc gaagcgatga gcctgctcgc
     1321 ggccgccgtc gtgacgaact cgacgatcac gatctgccga gtgccgatcg agttcctcga
     1381 gatcgagctg gcaacgctcg agggcatggg gatgcgctac gagatgacgc cggagtaccc
     1441 ctccgccaac gggcggaccc gactcgtcga cctgacgacg gttcccggcc cgctcaaggc
     1501 gccgggcgac aagatccacc cgatgccctt cccgggcctc aacatcgaca acctgccgtt
     1561 cttcgcgctg atcgcggcct cggccgtggg cacgacgatg ctgcacgact gggtctacga
     1621 gaaccgggcg atctacctca ccgagctcac caaggtcggc gccaaggtcc agctcctcga
     1681 cccgcatcgc gtcctcatcg aggggccgac ccgctggcga gccgccgagg tcatctgccc
     1741 ccccgccctg cgtccagccg tggtcgtgct cctggccatg ctcgccgccc cagggacctc
     1801 ggtcctgcgc aacgtctatg tcatcaaccg gggctacgag gagctcgccg agcggctcaa
     1861 cgccctgggc gcgaccatcg aggtcttccg cgacatctga gcggcccggc accctcgccc
     1921 ggcccgggcc cgcggtcagg cgcgcagagc ctcgctgagt cggtcggcaa cggcctcggg
     1981 ggtgaggccc gcctcctcga gaacctcacc gcgtgatccg tggccgagga acgccttggg
     2041 tatgccgtac aggtgcaccg gcacgtcgag cccgccgtcc cgcagcgcct gggcgatctg
     2101 agagcccact cccccgatga cgaggttgtc ctcgatcacc gcgacggccc gggcgtcccg
     2161 ggcgaggtcg accaggccgg cgctcacggg caaggcccac cgagggtcga ccacccgcac
     2221 ggcaaagccc tgggcttcga gcttggcggc cgcggtcagc gccaccgacg ccatcgcccc
     2281 gagaccgacg accaggacgt cgacgccgtt accgtccggg tcagggtcgc gcaggatgtc
     2341 gacctgcccg gtgacccgaa ccgccttgat gggcgccggg gggttgcctt tcgagaagcg
     2401 gatcacgctg ggtgcgccgc tgatggcgac cgcctcacgc agcgcccggc gcacctggac
     2461 ac
//
LOCUS       DATAIX010000388         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_110761, whole genome shotgun sequence.
ACCESSION   DATAIX010000388 DATAIX010000000
VERSION     DATAIX010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110761"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1532)
                     /locus_tag="VHM65_05840"
     CDS             complement(90..1532)
                     /locus_tag="VHM65_05840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2705260.1"
                     /translation="MRTLSSWPLSRKLVTAMVALFVVVTAMTGVVTVLAVRTSAITQV
                     DEKLLTARQPLTNLRDADRPGGGQGGGGDGPTGLGRGLAYVDLGATTIARVNTGVNTS
                     QNLTADQVSAIITAADGSEPVTVGLGDGLDDYRFVKSTDQRGGTIYVGLPLRDAYETV
                     RNLVRALVLLAGLGVILIAAGGLWAVRATLRPLQRVAATATRVSRLELAEGEVALAER
                     VPVQYTDPRTEVGQVGLALNNLLDTVGSALNARHRSEQRVRQFVADASHELRTPLASI
                     RGYAELSRREKEPVPASVTHALGRVESEATRMSQLVEDLLLLARLDAGRPLVREPVDL
                     TQLTVDAVSDAHAASPEHRWQLDLPDEPVEISGDPARLHQIIANLLANARTHTPAGTT
                     VRAGLATVPEGVRISVWDNGPGVPKALQVNVFERFARGDTSRNRAAGSTGLGLSIVAA
                     VAQAHGGRVELDSQPGHTTFTVTLPAQAAS"
     gene            complement(1549..2289)
                     /locus_tag="VHM65_05845"
     CDS             complement(1549..2289)
                     /locus_tag="VHM65_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2705261.1"
                     /translation="MSTLPWAKAHKLERPDGSPPRVLVVDDEHNLTELLSMAVRYEGW
                     EVRTAANGMAAVRAAREFEPDAVALDMMLPDMDGLEVLRKMRQNNDRIAVLFLTARDA
                     VEDRIAGLTAGGDDYVTKPFSLEEVIARLRALMRRTATVAAEEDDSILVVGDLTLDED
                     SHEVTRAGAQIALTATEFELLRYLMRNPRRVLSKAQILDRVWNYDFGGQANVVELYIS
                     YLRKKIDAGREPMIHTMRGVGYVLKPAS"
     gene            complement(2294..>2656)
                     /locus_tag="VHM65_05850"
     CDS             complement(2294..>2656)
                     /locus_tag="VHM65_05850"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2705262.1"
                     /translation="LQHELRISSGATTAVIDRLERHGYAVRERDTVDRRKVHLRHGDR
                     ARQVGADFFGPLGVLSDRVMGEFTDTQLETIRRFLALMAEAYAQHADTVDRRQPGTGV
                     TQQSHRKDMPPSQGVPLD"
BASE COUNT          349 a          862 c         1020 g          425 t
ORIGIN      
        1 gtcctgaccc atgctgagac ctcgctgtgc gatctctgtt ggcgaccgat gggtatgccg
       61 ggcacgctcg gcatacctct gctcgccgct cacgacgcgg cctgggccgg cagggtgacc
      121 gtgaaggtcg tgtgcccggg ctgcgagtcg agctcgacgc ggcccccgtg ggcctgggcg
      181 acggctgcca cgatgctgag tcccagcccg gtggaccccg ccgctcggtt gcgggaggtg
      241 tcgccgcggg cgaaccgctc gaagacgttg acctgcagcg cttttggcac cccagggccg
      301 ttgtcccaca cgctgatccg cactccctcc gggactgtgg ccaggcccgc ccgcacggtc
      361 gtgcccgccg gtgtgtgggt ccgggcgttg gcgaggaggt tggcgatgat ctggtgcagc
      421 cgggccgggt cgccgctgat ctcgaccggc tcgtcgggca ggtcgagctg ccagcggtgc
      481 tcgggggagg cggcatgggc gtcactgacg gcgtcgacgg tgagctgggt caggtcgaca
      541 ggctcgcgca cgagggggcg tccggcgtcg agacgggcca ggagcagcag gtcctcgacg
      601 agctgggaca tgcgggtcgc ctcggactcg acccgtccga gcgcgtgggt gacagatgct
      661 ggcacgggct ccttctcgcg gcgggacagc tcggcatacc cccggatcga ggcgagtggc
      721 gtgcgcagct cgtggctcgc atccgcgacg aactggcgca cgcgctgctc gctgcggtgc
      781 cgcgcgttga gggcgctgcc gaccgtgtcg agcaggttgt tgagcgcgag cccgacctgg
      841 ccgacctcgg tccgggggtc ggtgtactgc accgggacgc gctcggcgag ggcgacctcg
      901 ccctcggcga gctcgaggcg ggacaccctc gtcgccgtgg ccgcgacccg ttgcaggggg
      961 cgcagcgtgg cgcggacggc ccagagtccg cctgcggcga tgaggatgac gcccaggccg
     1021 gccagaagca cgagggcccg gacgaggttt ctgacggtct cgtaggcgtc acgcaggggc
     1081 aggccgacgt agatggtgcc gccgcgctgg tcggtcgact tgacgaagcg gtagtcgtcg
     1141 aggccgtccc ccaggcccac cgtgaccggc tcgctgccgt ccgcggccgt gatgatggcg
     1201 ctgacctggt cggccgtgag gttctgggag gtgttgaccc cggtgttgac ccgggcgatc
     1261 gtcgtggcgc ccaggtcgac atacgcgaga ccgcggccca ggcccgtggg gccgtcgcct
     1321 ccgccgccct gcccgccgcc gggacggtcg gcgtcccgca ggttggtgag gggctggcgg
     1381 gccgtcagga gcttctcgtc gacctgggtg atcgcgctgg tccgcacggc gagcaccgtc
     1441 accacgccgg tcatcgccgt cacgaccacg aagagggcga ccatggcggt gacgagcttg
     1501 cggctcagcg gccaggacga gagggtgcgc atggggacct tcgtcgggtc aggaggccgg
     1561 cttgaggacg tatccgacac cccgcatggt gtggatcatc ggctcgcggc cggcgtcgat
     1621 cttcttgcgc aggtaggaga tgtagagctc gacgacgttg gcctggccac cgaagtcgta
     1681 gttccacacc cggtcgagga tctgggcctt ggacaggacc cgcctggggt tgcgcatgag
     1741 gtagcgcagc agctcgaact cggttgcggt gagggcgatc tgcgcaccgg cgcgagtgac
     1801 ctcgtggctg tcttcgtcga gggtgaggtc accgacgacg aggatggagt cgtcctcctc
     1861 ggcggcgacc gtggcggtcc ggcgcatgag ggcgcggagc cgggcgatga cctcctcgag
     1921 gctgaacggc ttggtcacgt agtcgtctcc gccagccgtg agaccggcga tccggtcctc
     1981 gacggcatcg cgagcggtga ggaagagcac cgcgatccgg tcgttgttct ggcgcatctt
     2041 gcgcaggacc tcgaggccgt ccatgtcggg cagcatcatg tcgagggcga cggcgtcggg
     2101 ctcgaactcg cgggcggcgc gcacggcagc catcccattg gccgcggtcc ggacctccca
     2161 cccctcgtag cgaaccgcca tgctgagcag ctcggtgagg ttgtgctcgt cgtcgacgac
     2221 gagcacgcgc ggggggctgc cgtcggggcg ctcgagcttg tgggccttgg cccagggaag
     2281 ggtgctcata ggcctagtca aggggtactc cctgagacgg gggcatgtcc ttcctgtggc
     2341 tttgctgtgt gacgcctgtg ccgggctggc gccgatcgac cgtgtcggca tgctgggcgt
     2401 acgcctcggc catgagcgcg aggaagcgcc ggatcgtctc gagctgggtg tcggtgaact
     2461 cacccatgac cctgtcgctg agcacgccga gcggaccgaa gaagtcggca cccacctgac
     2521 gcgcccggtc accgtggcgc aggtggacct tgcggcggtc gaccgtgtcg cgctcgcgca
     2581 cggcgtagcc gtggcgctcg agccggtcga tgactgccgt ggtcgcccct gaggagatcc
     2641 gcagctcgtg ctggag
//
LOCUS       DATAIX010000389         2575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_51225, whole genome shotgun sequence.
ACCESSION   DATAIX010000389 DATAIX010000000
VERSION     DATAIX010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51225"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2575)
                     /locus_tag="VHM65_05855"
     CDS             complement(<1..>2575)
                     /locus_tag="VHM65_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HEX2705263.1"
                     /translation="IPGVVDGLAGGRLLATAATITAILAASSVHDADSVATWHRALST
                     MAGVRATLEVFQPEVFDVPLDEHVAATAPRGDHSEAPGVDLGWFARWRVRSQTRRLLR
                     PGRPPRDLYGELLKARRERNDWTALTGAGGRPEISPRLDVAAAALDSIRAGLLWLDER
                     LPRLPGGGGLLDLPAQDLQERVDALAARPERLAIRPQVTRAFDGFAAVGLGDLVEDLA
                     SRRVAPGEVVAELDYVWWASLAQELANSDTRYAHHNGPALHRVAEAYREADLSYLDQS
                     AGRVRAAVAQRLRETLADHPEHEAMVRAEAAKSRRHRSIRELMPVVGDTLTAIKPCWV
                     MSPLVVASVLPPGRWFDVVVFDEASQVQPAEAMSAISRGSHVVVAGDNRQLPPTSFFT
                     SVVDEEPAPLGEGSLTEGVESILDALAAIVPTRRLTWHYRSRDERLIAFANATMYDGS
                     LVTFPSTGSDAVVTLESVEGTALLDAGDDAVIETTTSEVERVVALVLEHARTRPGESL
                     GVIALGITHATRIAEALRDALRGAGDVAGFFAQTRPERFFVKNLERVQGDERDAVIVS
                     IGFGKTLHGRVLHRFGPLNLEGGERRLNVAITRARQRLTVVSALRAEDLDPTRLRSAG
                     ALMLRDFLAYAASGGDRSADSAAVAPLAHELAERLRAHGAVVHEGYGHGSQRVDLAVE
                     DGMAPGRMRVAVELDGPAYAQIPGVRDRERLRPQHLERLGWTHLRVWGTDVFRDPARD
                     VARVIAAARVTVPDGAPTRSAAATTENATEAGAGTMHSADPEPTAAQVEAYVSQVGAA
                     EVGATEAGDSAEVPATEAESAQDVPAEPDDTVDAEPEPDDTVDAEPVPTVPAGAEPEP
                     SA"
BASE COUNT          332 a          926 c          983 g          334 t
ORIGIN      
        1 gcactcggct cgggctcggc acctgcgggc acggtcggca ccggctcggc gtcgacggtg
       61 tcgtccggct cgggctcggc gtcgacggtg tcgtccggct cggccgggac atcctgagcc
      121 gactcggcct cagtggccgg aacctcggcg ctgtctcccg cctcggttgc accgacctcg
      181 gctgcaccga cctggctcac atacgcctcg acctgtgccg cggtcggctc aggatcggcc
      241 gagtgcatgg tgccggcccc cgcctccgtg gcgttctcgg tcgtggcagc ggccgaacgg
      301 gtcggcgcgc cgtcgggtac ggtgaccctg gcggccgcga tgaccctggc cacgtcccgt
      361 gccgggtccc ggaagacgtc ggtcccccac acccgcaggt gggtccaccc gagtcgttcg
      421 aggtgctgcg gacgcagccg ctcccggtcg cggacgccgg ggatctgggc atacgccggc
      481 ccgtcgagct cgaccgccac gcgcatccga cctggcgcca taccgtcctc gacggcgagg
      541 tcgacccgct ggctgccgtg gccatagccc tcgtggacca ccgcaccgtg cgcccgcagc
      601 cgttcggcca gctcgtgcgc gaggggcgcc accgccgccg agtcggccga gcggtcgccg
      661 ccggatgcgg catacgcaag gaagtcccgc agcatgagcg cacctgcgga cctgagccgc
      721 gtggggtcga ggtcctcggc ccgcagcgcc gagacgacgg tgagccgctg gcgcgcccgg
      781 gtgatggcga cgttgagacg gcgttcgccc ccctcgaggt tcagcgggcc gaagcggtgc
      841 aggacccgcc cgtgcagggt cttgccgaag ccgatcgaca cgatgaccgc gtcccgttcg
      901 tcaccctgga cccgctcgag gttcttgacg aagaaccgct ccggccgggt ctgggcgaag
      961 aagcccgcga catccccggc cccgcgcagc gcatcgcgca gcgcctccgc gatccgcgtc
     1021 gcgtgggtga ttccgagggc gatgacgccg agcgactcgc cggggcgggt ccgggcatgc
     1081 tcgaggacga gcgcgacgac gcgttcgacc tcgctcgtcg tcgtctcgat caccgcgtcg
     1141 tcaccggcgt cgaggagcgc ggtgccctcc accgactcga gcgtcaccac ggcatcggac
     1201 ccggtgctcg ggaaggtcac cagcgacccg tcatacatcg tggcgttggc gaaggcgatg
     1261 agccgctcgt cacgcgaccg gtagtgccag gtgaggcgcc gggttggcac gatggccgcg
     1321 agggcgtcga ggatcgactc gacgccttcg gtgagggagc cttcgccgag cggggcgggc
     1381 tcctcgtcga cgaccgaggt gaagaagctc gtcgggggca gctggcggtt gtcaccggcg
     1441 acgacgacgt ggctgccccg ggagatcgcc gacatcgcct cggccggctg gacctgcgag
     1501 gcctcgtcga agacgacgac gtcgaaccag cgccccggtg gcagcaccga cgcgacgacg
     1561 agcggggaca tgacccagca cggcttgatc gcggtgaggg tgtcaccgac gacgggcatg
     1621 agctcgcgga tcgagcggtg ccggcggctc ttggcggcct cggcgcggac catcgcctcg
     1681 tgctcggggt ggtcggccag ggtctcgcgc aggcgctgcg ccaccgcggc gcgcacccgc
     1741 ccggcggact ggtcgagata gctgaggtcg gcctcacggt aggcctcggc gacccggtgc
     1801 agggccgggc cgttgtggtg ggcgtagcgg gtgtcgctgt tggcgagctc ctgggcgagc
     1861 gaggcccacc agacgtagtc gagctcggcg acgacttcgc cgggggcgac ccgcctcgag
     1921 gcgaggtcct cgacgaggtc gccgagcccg acggccgcga agccgtcgaa cgcccgggtc
     1981 acctggggcc ggatcgccag ccgctcgggc cgcgcggcga gagcgtcgac ccgctcctgc
     2041 agatcctgcg ccggcaggtc gagcagcccg cccccgccgg gaaggcgggg cagccgctcg
     2101 tcgagccaga gcagcccggc ccggatcgag tccagggccg cggccgcgac gtcgagccgg
     2161 ggcgagatct cgggccggcc gcccgcgccg gtgagggcgg tccagtcgtt gcgctcccgg
     2221 cgggccttga gcagctcgcc atacaggtcc cgtgggggtc gtccgggccg cagcagccgc
     2281 cgggtctgcg agcgcacccg ccatcgggcg aaccagccga ggtcgacgcc tggggcctcg
     2341 gagtggtcgc cccggggagc ggtggccgcg acgtgctcgt cgagcgggac gtcgaagacc
     2401 tccggctgga agacctcgag ggtggcccgg accccggcca tcgtcgacag ggcgcggtgc
     2461 caggtcgcga cgctgtcggc gtcgtggacc gaggacgcgg cgaggatcgc cgtgatcgtg
     2521 gccgcggtgg cgagcaggcg cccaccggcc agcccgtcga cgacccctgg tatgg
//
LOCUS       DATAIX010000390         4397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_170618, whole genome shotgun sequence.
ACCESSION   DATAIX010000390 DATAIX010000000
VERSION     DATAIX010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170618"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VHM65_05860"
     CDS             complement(<1..523)
                     /locus_tag="VHM65_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2705264.1"
                     /translation="MIRFDSVTKTYPDGTVAVGGLSLDAPSGKITVLVGPSGCGKTTC
                     LRMVNRMIEPTSGQILIDDVAVATGDAAALRRGIGYVIQHAGLFPHRTVVDNVATVPL
                     LLGWDRRRARSRALELLERVGLPEAMAGRYPAQLSGGQQQRVGVARALAADPPVMLMD
                     EPFGAVDPIVRERL"
     gene            complement(626..1714)
                     /locus_tag="VHM65_05865"
     CDS             complement(626..1714)
                     /locus_tag="VHM65_05865"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX2705265.1"
                     /translation="MTRRTPLALAALAALALAACSGGAEAVTSVATTRPAATPTKAKA
                     KPTPAATPKPRRTPTPASGSASTLNLAFAGADLAVVNFETAITGRGTPEPKAFHFRTR
                     PKALMTLQKAGVDAVSLANNHAVDYGPLGLEDTLAARAASPIPVIGIGRNAEDAFAPA
                     TFTLKGVRVAVIASTQVNDLTVTKFPATDTRAGVAGNLTNTRLVAAVRKAAASYDVVV
                     VFLHWGTDYTSCPDGAQERTARDLEAAGADVIVGGHAHRVQAAGWFGRSYVGYGLGNF
                     VWHIRAEPDRRSGVLNVSIDIPRAKARGAATGAARMTTTSLVTAADWTPLHVDSDGVP
                     RQPSAGTASALYADWQARRACSNLKAQP"
     gene            1814..2419
                     /locus_tag="VHM65_05870"
     CDS             1814..2419
                     /locus_tag="VHM65_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX2705266.1"
                     /translation="MTVTGLLLAAGAGTRMGGPKALLTRADGSRYVDFALSALAEGGA
                     DEIVVVVGAEADAVRAHLSGYTGPASLPGEAVQVVVADQWATGMGSSLRAGLQAVQSR
                     PATCVVVALVDLPDVTGAVVARVLRAAGEGGSALARAAYHGVPGHPVVLGRDHWAGVL
                     AAAVGDRGARDYLARHAPALVECADLATGRDTDTPTDLALG"
     gene            2474..3460
                     /locus_tag="VHM65_05875"
     CDS             2474..3460
                     /locus_tag="VHM65_05875"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX2705267.1"
                     /translation="MHEPTALPSAPLSTRVTSASAFAALVGSTGYLCDDALATIGFLA
                     VRLERPLLLEGEPGTGKTALAEAIAQGLDIPLVRLQCYEGIDASQALYDWDFPRQILH
                     LRALEAVGRGMTAGRGMTDGQGMTDGQGMTDGSGPALDRVESELFTERFLLARPVLRA
                     LQQSPCVLLVDEVDRADDEFEAFLLEVLSTYQVSIPELGTVRAATPPVVVLTSNRTRE
                     VHDALKRRCLYHWIDHPGLARELAIVRSRAPEVSEALAEQVVTLVQRLRSAGDLIKPP
                     GIAETLDWARALDRLGTSELDIESAARTLGVAVKYREDAERVRALLDRMLSR"
     gene            3468..>4397
                     /locus_tag="VHM65_05880"
     CDS             3468..>4397
                     /locus_tag="VHM65_05880"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2705268.1"
                     /translation="MTSATVDLPPAVTPADGLLLGFARALRAAGVPVTPDRSATFLAA
                     VAAAGLGERAATYWAGRATLCACPDDLERYDQVFLAWFGAEALGAAAPAPTSRPRPQT
                     RVEADAESAADGGPDDTEELRVAASTVEILRHRDVAALTPAERRWLAEVLSALRPRVP
                     QRRATRLGPSYRGRLDPHRTLRAQLRQMGEPAALEWRRRRSRPRRVVLLVDVSGSMSP
                     YADVLLRVAHRYVRTGAPAEVFTVGTRLTRVTRALRQRDPDRALHDAGQAVPDWSGGT
                     RLAETLGVFVQQWGRRGAARGAVVVVCSDGWERG"
BASE COUNT          525 a         1563 c         1683 g          626 t
ORIGIN      
        1 ggaggcgctc gcggacgatg ggatcgaccg ccccgaacgg ctcgtccatg agcatgacgg
       61 gcgggtcggc cgccagcgcc cgcgccaccc cgacccgctg ctgctgaccg ccggagagct
      121 gtgcggggta gcgccccgcc atggcctcgg gcagcccgac ccgctccagc agctccagcg
      181 cccggctccg cgcccggcgc cggtcccagc cgagcaggag cggcaccgtg gcgacgttgt
      241 cgacgacggt ccggtgcggg aagagcccgg catgctggat gacgtagccg atccctcgtc
      301 gcaacgcggc ggcgtctccc gtcgcgacgg cgacgtcatc gatgaggatc tgccccgagg
      361 tcggctcgat catccggttg accatccgca ggcaggtggt cttgccgcag cccgacgggc
      421 cgacgaggac cgtgatcttg cccgatggcg cgtcgagact cagcccgccc acggcgacgg
      481 tcccgtcggg ataggtcttg gtcaccgagt cgaaacggat cacagtggcc ccacttccga
      541 gctgcgagtg cgggttagac cggatgggcg acagtcaggg ctacgacagt aggacatggc
      601 tccgacacga ccctgctggg gaagcctacg gctgcgcctt gaggttcgag cacgcgcgcc
      661 gggcctgcca gtcggcatac aaggcgctcg ccgtgcccgc cgagggctgg cgcgggacgc
      721 cgtcggagtc gacatggagc ggggtccagt ccgccgcggt gaccaggctt gtcgtcgtca
      781 ttcgggcggc gcccgtggct gccccgcggg ctttggcgcg ggggatgtcg atgctgacgt
      841 tgagcacgcc gctgcgccgg tcaggctccg cacggatgtg ccagacgaag ttgcccaggc
      901 cgtagccgac gtagctgcgc ccgaaccagc ccgccgcctg gacccggtgg gcatgcccgc
      961 cgacgatgac gtcggcgccg gccgcctcaa ggtcgcgggc cgtgcgctcc tgcgccccgt
     1021 cgggacagct cgtgtagtcg gtcccccagt gcaggaacac cacgacgacg tcgtatgacg
     1081 cggcggcctt gcgcacggcg gccacgagcc gtgtgttggt gaggttgccc gcgacccctg
     1141 ccctggtgtc ggtggctggg aacttggtga cggtgaggtc gttgacctgg gtcgaggcga
     1201 tgaccgccac gcgaacaccc ttgagggtga acgtcgccgg ggcgaaggcg tcctcggcgt
     1261 tgcggccgat gccgatgacg gggatcgggc tggccgccct ggccgcgagg gtgtcctcga
     1321 gcccgagagg cccgtagtcg accgcatggt tgttggccag gctgaccgcg tcgacaccgg
     1381 ccttctgcag cgtcatgagc gccttgggcc tggtgcggaa gtggaacgcc ttcggctcgg
     1441 gcgtcccgcg gcccgtgatc gcggtctcga agttgaccac ggcgaggtcg gctccggcga
     1501 aggcgaggtt gagcgtgctc gccgacccgc tggcgggggt aggcgtcctg cggggcttcg
     1561 gcgtcgccgc cggcgtgggc ttggccttgg ccttcgtcgg tgtggccgcc ggccgtgttg
     1621 tcgccacgga ggtgaccgcc tcggcgccgc ccgagcaggc ggccagcgcg agggcggcca
     1681 gagcggccag cgcgaggggg gtccgtcggg tcacccgact actgtgccat cccagggcca
     1741 caccgtcggt ccgacacgca tcggcccagg gccggcctgg cctggccgag cccgatcgga
     1801 gacgatgcac ggtatgacgg tcaccgggct gctgctcgcc gcgggcgcag gcacccggat
     1861 gggcggcccg aaggccctgc tcacccgggc ggacgggtcg cggtatgtcg acttcgctct
     1921 gtcggcactg gccgaggggg gtgccgacga gatcgtcgtc gtcgtgggtg ccgaggccga
     1981 cgccgtgcgt gcgcacctga gcggctacac cgggcccgcg tccctgcccg gcgaggcggt
     2041 gcaggtggtc gtggcggacc agtgggcgac cgggatgggc tcctcactcc gggcgggtct
     2101 gcaggcggtg cagagccggc ccgcgacgtg cgtcgtcgtc gcgctggtcg accttcccga
     2161 tgtcacgggg gccgtcgtcg cccgggtgct ccgtgccgcc ggtgaggggg gctccgcgct
     2221 ggcgcgggcg gcataccacg gggtgcctgg tcatcccgtg gtgctgggac gcgaccactg
     2281 ggccggcgtg ctcgcggccg ccgtgggcga ccggggggcc cgggactacc tggcgaggca
     2341 cgcgccagcg ctcgtcgagt gcgctgacct cgccacgggt cgagacaccg acacgcccac
     2401 cgacctggcg ctcggctgag tggtctgcgc agcgtcactc cggcccgtgc ggcggtggtc
     2461 catgcttggg tccatgcacg agccgaccgc cctgccgagc gcgccgctgt cgacccgggt
     2521 gacctcggcg agtgccttcg ccgccctcgt cggctcgacc ggctaccttt gcgacgacgc
     2581 gctcgccacg atcggcttcc tcgccgtgcg gctggagcgg ccgctgctgc tcgaaggcga
     2641 gcccgggacc ggcaagaccg ccctcgccga ggccatcgcg caaggcctgg acatcccgct
     2701 cgtgcggctg cagtgctacg agggcatcga cgcctcgcag gccctctacg actgggactt
     2761 cccgcgccag atcctgcacc tgcgcgcgct ggaggcggtc gggcgcggca tgacggccgg
     2821 gcggggtatg acggatggac agggtatgac ggacgggcag ggtatgacgg acgggtccgg
     2881 tcccgctctg gaccgggtcg agtccgagct gttcaccgag cggttcctgc tcgcccgtcc
     2941 cgtgctgcgt gccctgcagc agagcccgtg cgtcctgctg gtcgacgagg tcgaccgcgc
     3001 cgacgacgag ttcgaggcgt tcctgctcga ggtgctctcg acctaccagg tctcgatccc
     3061 ggagctgggc acggtccggg ccgcgacccc cccggtcgtc gtgctcacgt ccaaccgcac
     3121 ccgggaggtc cacgacgcgc tcaagcgccg ctgcctctac cactggatcg accatcccgg
     3181 cctggcgcgt gagctcgcga tcgtccggag ccgggcgccc gaggtgagtg aggcgctggc
     3241 cgagcaggtc gtcacgctcg tgcagcggct gcggtcggcg ggagacctca tcaagccccc
     3301 ggggatcgcc gagacgctcg actgggcgcg ggcactggac cggctcggga cgagcgagct
     3361 cgatatcgag tcggcggccc gcacgctcgg cgtggcggtg aagtaccgcg aggacgccga
     3421 gcgggtccgt gccctgctcg accggatgct gtcgcggtga ccgggccatg acgtcggcca
     3481 ccgtggacct cccacctgcc gtgacccccg cggacgggct tctcctcggg tttgcccggg
     3541 cgcttcgggc cgcgggagtg ccggtgaccc cggaccgctc ggcgaccttc ctcgccgcgg
     3601 tcgcggccgc cggcctgggc gagcgggccg cgacgtactg ggcgggccgg gccaccttgt
     3661 gtgcctgccc ggacgacctc gagcgctacg accaggtctt cctcgcgtgg ttcggcgctg
     3721 aggcgctcgg tgccgccgcc ccggcgccga cctcgcggcc ccgtccgcag acccgtgtcg
     3781 aggccgacgc cgagagcgct gcggacgggg gcccggacga caccgaggag ctgcgcgtcg
     3841 ccgcgagcac ggtcgagatc ctgcgccatc gcgatgtggc ggccctgacc ccggcggaac
     3901 ggcgctggct cgccgaggtg ctctccgcgc tgcgcccccg ggttccgcag cggcgcgcga
     3961 cccggctcgg gccgtcatac cggggccgtc tcgacccgca ccgcaccctg cgcgcacagc
     4021 tgcgccagat gggtgagccg gccgcgctgg agtggcgacg ccgacgctcc cggccgcggc
     4081 gggtcgtcct gctcgtcgac gtgtcggggt cgatgagccc gtatgccgac gtgctcctgc
     4141 gggtggcgca tcgctacgtg cgcaccgggg caccggccga ggtcttcacg gtcgggacgc
     4201 ggctgaccag ggtcacccgg gcgctgcgcc agcgcgaccc tgaccgcgcg ctgcacgacg
     4261 cggggcaggc cgtcccggac tggtcggggg ggacccggct cgcggagacg ctgggtgtgt
     4321 tcgtccagca gtgggggcgc cggggggccg cccggggagc tgtcgttgtc gtgtgcagcg
     4381 acggctggga acgcggt
//
LOCUS       DATAIX010000391         1811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_217846, whole genome shotgun sequence.
ACCESSION   DATAIX010000391 DATAIX010000000
VERSION     DATAIX010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217846"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /gene="pyrH"
                     /locus_tag="VHM65_05885"
     CDS             complement(<1..234)
                     /gene="pyrH"
                     /locus_tag="VHM65_05885"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX2705269.1"
                     /translation="MTDVYPRRQFKRVVLKLGGEMFGGGEVGVDPDVVSAVAEQIAEV
                     AADGVQVAVVIGGGNFFRGAQLHERGMDRARADY"
     gene            complement(365..1186)
                     /gene="tsf"
                     /locus_tag="VHM65_05890"
     CDS             complement(365..1186)
                     /gene="tsf"
                     /locus_tag="VHM65_05890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX2705270.1"
                     /translation="MANFSAADVKRLRELTGAGMMDCKKALDENDGDFDKAVEFLRIK
                     GAKDIGKRAERTTANGLVASSGNALIELDCETDFVAKNDEFQQLAERIVTVVASAEVT
                     AASIDEVLGAKLGSETVADAVNALSAKIGEKLELRRVVKYDGNTAVYLHKRSADLPPA
                     IGVLVEYTGDNAEAARGAAMQIASLKARYVSRDAVPADIVESEKRIAEATAREEGKPD
                     AALPKIVEGRVNGFFKEAVLTEQPSVLDSKKTVGKLLDAAGVSVAAFSRFEVGTA"
     gene            complement(1320..>1811)
                     /gene="rpsB"
                     /locus_tag="VHM65_05895"
     CDS             complement(1320..>1811)
                     /gene="rpsB"
                     /locus_tag="VHM65_05895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX2705271.1"
                     /translation="IPSAVWIVDTKKEHIAVGEARKLNIPVVAVLDTNCDPDEVDYPV
                     PGNDDAIRSAALLTKVVAEAVAEGLLARSGGLSGSSSTEEPLAEWERELLASGAAEAE
                     AAAAAAESAAAPVEAAPIDAIAETEAGAEALLEVRADSAEGIDEIVPNEQVSALAPGS
                     DLN"
BASE COUNT          235 a          651 c          607 g          318 t
ORIGIN      
        1 gtagtccgcg cgggcccggt ccatcccccg ttcgtgcagc tgggcgccgc ggaagaagtt
       61 gccgccgccg atgaccaccg cgacctgcac cccgtcggcg gccacctcgg cgatctgctc
      121 ggcgaccgca gagaccacgt cagggtcgac gccgacctcg ccgccgccga acatctcacc
      181 gcccagcttg agcacgaccc gtttgaactg ccgcctggga tacacgtccg tcatgccgtc
      241 tccgccctcg ctgtgctgcg cctcgtcgcg gtcgcctgtc gtggtgggtg ccccgccctg
      301 ccgcaggcat accactgggg ccggtgctgc tcgcaccggc cccagtgtga ccgaactcgg
      361 ggtgtcaggc tgtgccgacc tcgaaccggc tgaacgccgc gacgctcacg ccggccgcgt
      421 cgagcagctt gccgacggtc ttcttggagt ccagcaccga cggctgctcg gtgagcacgg
      481 cctccttgaa gaacccgttg acccggccct cgacgatctt gggcagtgcg gcatccggtt
      541 tgccctcctc gcgggcggtc gcctcggcga tgcgcttctc ggactcgacg atgtcggccg
      601 ggaccgcgtc gcgcgagacg tagcgggcct tcagcgaggc gatctgcatg gccgcgccac
      661 gagcagcctc cgcgttgtcg ccggtgtact ccaccagcac gccgatggcc gggggcaggt
      721 cggcggaccg cttgtgcagg tagaccgcgg tgttgccgtc gtacttgacc acgcggcgca
      781 gctcgagctt ctcgccgatc ttcgccgaca gggcgttgac ggcgtcggcc accgtctcgg
      841 atccgagctt ggcgcccagc acctcgtcga tgctggccgc ggtcacctcg gcagaggcga
      901 ccacggtgac gatccgctcg gccaactgct ggaactcgtc gttcttggcg acgaagtcgg
      961 tctcgcagtc cagttcgatg agcgcgttcc cggacgaggc aaccaggccg ttggccgtgg
     1021 tccgctcggc gcgcttgccg atgtccttgg cgcccttgat gcgcaggaac tcgacggcct
     1081 tgtcgaagtc cccgtcgttc tcgtcgagag ccttcttgca atccatcatc ccggccccgg
     1141 tcagttcccg gagtcgcttg acgtcagcgg cactgaagtt cgccatgatc ggcgctgtcc
     1201 ttgtcttctc ggtcttctcg gtcgttgtcg atgatcagtt tcggaaattc gccgggcacg
     1261 acacggcaga cgaaccgctg tcccgacccg atgcggcgat cgggacggcg atggccgcgt
     1321 cagttcaggt cgctgcccgg ggccagggcg ctgacctgct cgttcggcac gatctcgtcg
     1381 atgccctcgg cactgtcggc ccggacctcg agcagtgcct cggcgccggc ctcggtctcg
     1441 gcaatggcat caatcggggc ggcctcgact ggcgcagcgg cactctcggc ggcagcggca
     1501 gcggcctcgg cctcggccgc gccgctggcc agcagttcgc gctcccactc cgccagcggc
     1561 tcctcggtgg aggagctgcc ggaaagccca ccgctgcggg ccagcaggcc ctcggcgacg
     1621 gcctcggcca cgaccttggt cagcagcgct gcggaccgga tggcgtcgtc attgccggga
     1681 accgggtagt cgacctcgtc ggggtcgcag ttggtgtcca gcaccgcgac gacggggatg
     1741 ttcagcttgc gggcctcgcc gacggcgatg tgctccttct tggtgtcgac gatccacacc
     1801 gcggagggga t
//
LOCUS       DATAIX010000392         2158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_110964, whole genome shotgun sequence.
ACCESSION   DATAIX010000392 DATAIX010000000
VERSION     DATAIX010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110964"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..881)
                     /locus_tag="VHM65_05900"
     CDS             complement(90..881)
                     /locus_tag="VHM65_05900"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX2705272.1"
                     /translation="MHEPASYYIPLGGGRYQPTRHTQGAWNPDEQHMGPVAGLMAHAI
                     DTHEPRPGMLLCRINYEILGMILAERNHIEVQTIRPGRTIELVEATLSVQDQPVIRAR
                     AWRLAAHDTAAVAGGSPEPIPGPDDMPEWLGTGVWGGGYIASLDFRAAAANRPGRGQA
                     WIRSRVGLVEGVESSDTARFVTLLDTANGIATRQDPRDWLYPNVDLGIHLHRQPRAGW
                     VGFDTSVAFGPAGLGVTSSTVYDVDGAVGRVEQMLTVRPTPSPGS"
     gene            complement(892..1791)
                     /locus_tag="VHM65_05905"
     CDS             complement(892..1791)
                     /locus_tag="VHM65_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705273.1"
                     /translation="MTIAFALPGVHSGPHDLKLGMAGPASAVAQVTGALAQNAPGAFE
                     PVTYADEAAVRSAITSRKIHGGIVVGQTGPKVLIASAGGTPIATVLRTVAANLGQSTG
                     TTIPVEDVVPYRAQDPNGAGLASVALPLSIGGIIPAVILLQLFTRSLALRLAGAIAFA
                     LVAAFFVIAILTFWTHTIGGSYVLTSLVFALGISAITLVALGLESLVGLPGLGLIAIL
                     MIFVANPLSGLATAPAWLPSPWGAIGQLMPPGASGTALRSFGFFDGQGAGAQLVTLAA
                     WAVAGLALAALGARRTTAPEPAA"
BASE COUNT          323 a          695 c          849 g          291 t
ORIGIN      
        1 ggattacacg gtacctgctg accggcagga gcggcggcat accgccaatt ctggcagggc
       61 atggcgatcg aggagaacgg gccgacgcgc tacgaccccg ggctgggcgt cgggcggacg
      121 gtgagcatct gctcgacgcg cccgaccgcg ccgtcgacgt cgtacacggt gctcgaggtg
      181 acaccgagcc cggccgggcc gaaggcgacg gaggtgtcga acccgaccca gccggcccgc
      241 ggctggcggt ggaggtggat gccgaggtcg acgttcgggt agagccagtc gcgcggatcc
      301 tggcgcgtgg caatgccgtt cgcggtgtcg aggagggtca cgaagcgcgc ggtgtcgctg
      361 ctctcgaccc cctcgacgag gccgacgcgg ctgcggatcc acgcctggcc acggcccggg
      421 cggttggctg cggcagcccg gaagtcgagc gaggcgatgt agccgccgcc ccagacaccc
      481 gtgccgagcc actctggcat gtcgtcgggt ccggggatcg gctcgggcga gcccccggcg
      541 acggcggcgg tgtcgtgggc ggcgagacgc cacgcgcggg cccggatcac cggctggtcc
      601 tgcactgaca acgtcgcctc gacgagctcg atcgtgcggc ccggcctgat ggtctggacc
      661 tcgatgtggt tccgctcggc caggatcatc ccgaggatct cgtagttgat ccggcagagc
      721 agcatcccgg ggcgcggctc gtgcgtgtcg atcgcgtgcg ccatgagccc ggcgacgggt
      781 cccatgtgct gctcgtcggg gttccaggcg ccctgggtat gccgggtggg ctggtagcgt
      841 ccgccgccga gtgggatgta gtagctggct ggttcgtgca cgacgcccag actaggcagc
      901 cggctccggt gctgtcgtgc gacgggcccc cagcgcggcg agggcgagcc cggcgaccgc
      961 ccaggcggcg agggtgacga gctgggcacc ggcgccctgg ccgtcgaaga agccgaacga
     1021 gcgcagtgcc gtcccggatg cgccgggggg catgagctgc ccgatggcac cccacggcga
     1081 cgggagccac gccggtgcgg tcgcgaggcc ggagagcggg ttggcgacga agatcatgag
     1141 tatggcgatc agcccgagac cgggcagccc gacgagcgac tcgaggccga gcgcgacgag
     1201 cgtgatcgcg ctgattccca gggcgaagac gagcgaggtc aggacgtagc tgccgccgat
     1261 cgtgtgggtc cagaacgtca ggatggcgat gacgaagaac gcggcgacca gggcgaaggc
     1321 gatggcgccg gccagccgca gggccaggct gcgggtgaag agctggagca agatgacggc
     1381 cgggatgatg ccgccgatcg agaggggcag ggccaccgag gccagcccgg ccccgttggg
     1441 gtcctgggcc cgatagggga cgacgtcctc gaccgggatc gtcgtgccgg tggactgccc
     1501 gaggttggcc gcgacggttc tcaggacggt ggcgatgggc gtgccgcccg ccgaggcgat
     1561 gaggaccttc gggccggtct ggccgacgac gatgccgccg tggatcttgc gggaggtgat
     1621 cgcgctccgg accgccgcct cgtcggcata cgtgacgggt tcgaacgctc cgggggcatt
     1681 ctgtgcgagg gcgcctgtca cctgggccac cgctgacgcg ggtcctgcca taccgagctt
     1741 gaggtcgtgt gggccgctgt gtacgccggg cagggcgaag gcgatcgtca tcagcccgac
     1801 cacgacagcg agtccgagga cgacgccggc aagctgtcgc agcccagcgg agcgggccgg
     1861 tgctggcgat gccggatggc ctggtgccga cgtggcgctc atgtcaaaac cccttacgaa
     1921 acggagaaac tcgagcagtc tattcggacg atccttctcc ggttatgctg cgggtgtcaa
     1981 gaggcggcga tgcctcgccg gcgggccgat gggcacgcca ccgaggccga ggagggctga
     2041 tgcgagccga tgcgagacgc aaccggcacg ggctggtcgc cgccgctcgc gagctgctca
     2101 tcgagcgcgg caccgacttc ccgctcgacg ccgtctcggc ccgtgcgggg gtcggcgc
//
LOCUS       DATAIX010000393         4940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_51450, whole genome shotgun sequence.
ACCESSION   DATAIX010000393 DATAIX010000000
VERSION     DATAIX010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51450"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VHM65_05910"
     CDS             complement(<1..233)
                     /locus_tag="VHM65_05910"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX2705274.1"
                     /translation="MFRNVMPRYEILSQDAMDKLDHGWRRIVSELGVEFMSDRALDVF
                     RAAGQRVEERTVFFDPDFILEQVAKAPHEFDAVS"
     gene            337..1092
                     /locus_tag="VHM65_05915"
     CDS             337..1092
                     /locus_tag="VHM65_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX2705275.1"
                     /translation="MSANGTQAVDRAAHLLSLVVQAQDALTFSDLCDRAGYPRSTTSR
                     LLAALERRRLTERSADGHWVPGPLFAQYAARHTVDTELARVASPFLERLGEATGETVN
                     LAVVRGGSVVQIAQVESRFMRGSRDWVGVDVPAHCSALGKVLCAYGAVQVPATALDTP
                     TEHSLTTAAAVRAQFPQICHRGYAVTQDELEIGLTGVAAPVAVGGAIVAALGVSGPSS
                     RLAADLPGTGHTVVAHALALSTQLGRTRKEGAA"
     gene            1089..1754
                     /locus_tag="VHM65_05920"
     CDS             1089..1754
                     /locus_tag="VHM65_05920"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HEX2705276.1"
                     /translation="MTPEEILQGLYDQTMVGNAPAVLDLTKAALGLGMEPGSLLFDAL
                     IPALEEVGARFERGDFFVPEMLIAGRAMAGSMEVLRPLLAETGVQTIGKFVMGTVKGD
                     VHDIGKNLVNIMLEGAGFEVIDLGVQVAPEKFVAAIETHQPDIVGFSAFLTTTMPMFK
                     ANINALQKAGLRDSVLVMVGGAPVTQEYADVVGADGYAADASATVKKAKSLLQHRRAT
                     VLA"
     gene            1751..2689
                     /locus_tag="VHM65_05925"
                     /pseudo
     CDS             1751..2689
                     /locus_tag="VHM65_05925"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
     gene            2686..4524
                     /locus_tag="VHM65_05930"
     CDS             2686..4524
                     /locus_tag="VHM65_05930"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HEX2705277.1"
                     /translation="MTGPGRVDLSFTPAERTVRVPPGVTVFDAASWNGVAIDSTCGGH
                     GTCKKCRIRIVDGHVPVSKLDVRAFTSAELTSGWRLACLAQATQNLGVEVPPLMTRPK
                     AATVGAGRQVILRPAVQKRYLELAEPSLADQRTDLARVLDAIDDLELTADLEMLRRLP
                     AILRSSDFRVTAVVVDEALIDIEPGDTSGRRYGIAVDLGTTTVVATLLDLGTGTPVAV
                     SSMLNKQQPFGADVITRISATMMDPDALPRLRGLAQATLAELAAEVCAEAGVGPAEVY
                     EVALAGNATMTALLLGVDPEPLGVAPFIMSSASWPTLRATELGLSLHPGARALLFPAL
                     GAYVGGDIVAGMLAAGMDRDKRTRLFIDVGTNCEIVLSDGETIVATAAPAGPAFEGGA
                     IRCGMRAADGAIEVVRLGDDVELRVIGDVAPAGLCGSGLVDAVAELVRVGLLDTTGRL
                     VPEDAAAVLVPALAGRLTMIGQERVFVLHYPYDGAPPDECVYLSQRDVRELQFAKAAI
                     ATGWSLLLEELGVEPRDVQQVLLAGSFGSYLSASSAIRIGLVPALPALRIVSAGNVAG
                     EGAKMALLSSRERAGADALLKEVRYVELSDRVDFNDRFVDLLSFPG"
     gene            4538..>4940
                     /locus_tag="VHM65_05935"
     CDS             4538..>4940
                     /locus_tag="VHM65_05935"
                     /inference="COORDINATES: protein motif:HMM:NF019413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HEX2705278.1"
                     /translation="MALVACGAIAGPAADAVARHTWPVDVFPLPPLLHNQPQRIAGEV
                     ERLALDLRERYGRVVVGYADCGTYGALDGVCERLGLARLAGLHCYDLYAGAERMRAIF
                     EAEPGTYVLTDFLVRSFARTVIAELGLDRYPE"
BASE COUNT          676 a         1835 c         1688 g          741 t
ORIGIN      
        1 gagacagcgt cgaactcgtg cggcgccttg gcgacctgct cgaggatgaa gtcggggtcg
       61 aagaagaccg tgcgctcctc gacgcgctgc ccggcagccc ggaagacgtc gagggcccgg
      121 tcgctcatga actcgacacc cagctcgctg acgatgcgcc gccagccgtg gtcgagcttg
      181 tccatcgcgt cctggctcag gatctcgtag cgcggcatga cgttgcggaa catggacgac
      241 tcctcggctt ccttgacggc ccgtcgcggc acatcctagc ctcatgatgt gggacatcag
      301 ttccacatca tgaggctaag ggcggggacg cacgacatgt cagccaacgg cacccaggcg
      361 gtcgaccggg cggcgcacct gctgtccctc gtcgtccagg cccaggacgc cctgaccttc
      421 tccgacctgt gcgaccgggc cggctacccc cgctcgacga cctcgcgcct gctcgccgcc
      481 ctggagcgcc gccgtctcac cgagcgatcg gccgacggtc actgggtgcc gggcccgctc
      541 ttcgcccagt acgccgcccg ccataccgtc gacaccgagc tggcccgggt cgcctcgccc
      601 ttcctggagc gcctcggcga ggccaccggc gagaccgtca acctcgccgt cgtccggggc
      661 gggtcggtcg tccagatcgc ccaggtcgag agccggttca tgcgcggcag ccgcgactgg
      721 gtcggggtcg acgtcccggc ccactgctcg gcactgggca aggtcctttg tgcgtatggc
      781 gccgtgcagg tccccgccac ggccctcgac acgcccaccg agcactcgct gacgaccgcg
      841 gccgccgtcc gcgcccagtt cccgcagatc tgccaccgcg ggtatgccgt cacccaggac
      901 gagctcgaga tcggcctgac cggggtcgcg gcccccgtcg cggtcggcgg agccatcgtc
      961 gccgcgctcg gtgtttccgg gccgagctcg cgcctcgccg cggacctgcc gggcaccgga
     1021 cacaccgtcg tggcccacgc tcttgccctg tccacccagc tcggccggac ccgcaaagaa
     1081 ggagcagcat gacccccgag gagatcctgc aagggctcta cgaccagacg atggtcggca
     1141 acgcaccggc cgtgctcgac ctcaccaagg cggcgctcgg cctcgggatg gagccgggct
     1201 cgctgctctt cgacgcgctg atccccgcac tggaggaggt cggcgcccgc ttcgagcggg
     1261 gcgacttctt cgtgcccgag atgctcatcg ccgggcgcgc catggccggc tcgatggagg
     1321 tgctgcgccc gctgctcgcc gagaccggcg tccagacgat cggcaagttc gtcatgggga
     1381 cggtcaaggg cgacgtccac gacatcggca agaacctcgt caacatcatg ctcgagggcg
     1441 ccggtttcga ggtcatcgac ctcggcgtcc aggtggcgcc ggagaagttc gtcgccgcca
     1501 tcgagacgca ccagcccgac atcgtcggct tctcggcctt cctcacgacg acgatgccga
     1561 tgttcaaggc caacatcaac gcactgcaga aggccggcct gcgcgactcg gtcctcgtca
     1621 tggtcggcgg ggccccggtc acccaggagt acgccgacgt cgtcggcgcc gacgggtatg
     1681 ccgcggacgc ctccgccacg gtcaagaagg ccaagtccct cctgcagcat cgccgggcga
     1741 cggtgctggc atgacccgct atgacggccc ggtccgcgag acggtcctgc gctcggccac
     1801 ccggcaggtc gtcatcgcga gcaaccgccc attcaccgtc atcggcgagc ggatcaaccc
     1861 gaccgggcgc aaggtcttcc aggagcagct gcgcgccggc gacctctcgc tcatcgagaa
     1921 ggacgtccgg gcccaggtcg ccggcggcgc cggcgtcctc gacgtcaaca tgggcgtgcc
     1981 actcaccgac gaggccgacc tgctcgtccg cgccatcgtg ctggtccagt ccctcaccga
     2041 cctcccgatc tgcatcgact cctccgtcgt cgaggcgctg gaagccgggc tcgcggcata
     2101 acacggccgt gccctcgtca acttggtgac cgccgaggac gagcggctcg aggcgatcct
     2161 gcccctcgtc aagaagtacg acgccgccgt catcgccctg cccaacgacc acgacgagat
     2221 cccgatggag gcggacaagc gggtcgagct gaccgccaag atcgtgcggg tggcgacgca
     2281 ggagtacggc atcgcgatcg aggacatcgt catcgacccg ctcgcgatgc cgatcggcgc
     2341 cgactcctcg gtggggtgca cggcgctcga gacgatccgg cgggtccacg agcggtgggg
     2401 cctgaacacg acgtgcggcg cctcgaacat ctccttcggt atgccggccc gccccgcgat
     2461 caacgcgtcc tggctcgcgc tggccatggg ggccgggatg acgagcgcca tcatggacgc
     2521 gcgcacaccg cagatcgtcg agtcggtccg ggccaccgac ctgctgctcg gccatgacga
     2581 gtggggcggg agctggatcg cggcccaccg cgcgagacag gccgctgcgg ctactgcggc
     2641 tactgcgggt gccgctgccg ctgcggccct cgaagccgcc ccgacgtgac cggtcccggc
     2701 cgggtcgacc tgagcttcac ccccgcggag cggaccgtcc gggtccctcc gggcgtcacg
     2761 gtcttcgacg cggccagctg gaacggcgtc gccatcgact cgacgtgcgg cgggcacggg
     2821 acgtgcaaga agtgccgcat ccggatcgtc gacgggcatg tgccggtatc caagctcgac
     2881 gtccgggcgt tcacctcggc cgagctcacg tcgggctggc gcctcgcctg cctggcccag
     2941 gccacccaga acctcggcgt cgaggtgccg ccgctcatga cccggcccaa ggccgcgacc
     3001 gtcggcgccg ggcgccaagt catcctgcgc cccgcggtcc agaagcgcta tctcgagctg
     3061 gccgagccga gcctggccga ccagcgcacc gacctggccc gggtgctcga cgccatcgac
     3121 gacctcgagc tgaccgccga cctcgagatg ctgaggcgac tgcccgccat cctgcgctcc
     3181 tcggactttc gggtgaccgc ggtcgtcgtc gacgaggccc tcatcgacat cgagccgggg
     3241 gacacctccg ggcggcggta cgggatcgcc gtcgacctcg gcacgaccac cgtcgtggcg
     3301 acgctgctcg acctcggcac cggcacgccg gtggccgtct cctcgatgct caacaagcag
     3361 cagccgttcg gcgccgacgt catcacccgg atcagcgcga cgatgatgga cccggatgcg
     3421 ctgccccggc tccgtgggct ggctcaggcc accctggccg agctggccgc ggaggtctgc
     3481 gccgaggccg gggtcgggcc ggccgaggtt tacgaggtcg ccctcgccgg caacgcgacg
     3541 atgaccgcgc tgctgctcgg cgtcgacccc gagcccctcg gcgtcgcgcc gttcatcatg
     3601 tccagcgcct cctggccgac cttgcgggcg accgagctgg ggctgtcgct gcatcccggc
     3661 gcgcgcgccc tcctcttccc cgcactcggg gcctacgtcg gcggcgacat cgtcgcgggc
     3721 atgctcgccg ccgggatgga ccgcgacaag cggacccggt tgttcatcga cgtcgggacc
     3781 aactgcgaga tcgtcctgtc cgacggcgag acgatcgtcg cgacggccgc acccgcaggg
     3841 ccggccttcg agggcggggc catccggtgc gggatgcgcg ccgccgacgg cgccatcgag
     3901 gtggtccgcc tcggtgacga cgtcgagctg cgggtcatcg gtgatgtcgc tccggcaggg
     3961 ctgtgcggct ccggtctcgt cgacgcggtg gccgagctgg tgcgtgtcgg gctgctcgac
     4021 accaccggcc ggctcgtccc cgaggacgcc gcggcggtac tcgtcccggc gctcgccggg
     4081 cggctcacga tgatcggcca ggagcgggtc ttcgtcctgc actacccgta tgacggggcg
     4141 ccgcccgacg agtgcgtcta cctgtcccag cgcgacgtcc gcgagctgca gttcgccaag
     4201 gccgcgatcg ctaccggctg gtcgctgctc ctggaagagc tcggtgtcga gccgcgagac
     4261 gtgcagcagg tgctcttggc gggctcgttc gggagctacc tgtcggcgtc ctcggcgatc
     4321 cggatcggtc tcgtccccgc gctgcccgcc ctgcgcatcg tctccgctgg caacgtcgcc
     4381 ggcgaggggg ccaagatggc gctgctcagc tcacgtgagc gggccggtgc cgacgcgctg
     4441 ctcaaggagg tgcgctatgt cgagctctcc gaccgggtcg acttcaacga ccgcttcgtc
     4501 gacctgctct ccttccccgg ctgacctgtc cgcccgcatg gcgctcgtcg cgtgcggcgc
     4561 catcgccggg ccggccgcgg atgccgtggc gcgccatacc tggccggttg atgtcttccc
     4621 gctgcctccg ttgctgcaca accagccgca gcgcattgcg ggggaggtgg agcggctggc
     4681 cctggacctg cgcgaacgct acggccgcgt cgtcgtcggc tatgccgact gcgggacgta
     4741 tggcgcgctc gacggcgtgt gtgaacggct cgggctcgcc cggctcgcgg gcctgcactg
     4801 ctatgacctc tatgccgggg ccgagcggat gcgggcgatc ttcgaggccg agccggggac
     4861 ctatgtcctg accgacttcc tcgtgcgctc gttcgcccgg acggtcatcg ccgagctcgg
     4921 gctcgatcgc tatccggagc
//
LOCUS       DATAIX010000394         3783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_111137, whole genome shotgun sequence.
ACCESSION   DATAIX010000394 DATAIX010000000
VERSION     DATAIX010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111137"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VHM65_05940"
     CDS             <1..235
                     /locus_tag="VHM65_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX2705279.1"
                     /translation="PAPGRSQEIALVGFDDFPLADLLEPALTVVRQDVRIVAERVCEL
                     VFARVEGDTSPPQHVVVATTLVTRGSGEIPPRT"
     gene            380..1471
                     /locus_tag="VHM65_05945"
     CDS             380..1471
                     /locus_tag="VHM65_05945"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX2705280.1"
                     /translation="MLATTSRRSGALALAGVVAAGLSLAACSSSSSTSSPSGAAGGDA
                     VAVSLITKDSTNPFFVAMQKGAKEAAEKNGVALTVASGSKDGDEAGQITAIENAIAAQ
                     AKGILITPNGPGVNSAIKKAREAGLYVIALDTPPDPADTVDITFATDNFLAGKLIGQW
                     AKTQLAGKDATIALLDLFNDKVVSVDYNRDQGFLAGMGIDTKDAKKNGDEDKTGSYAG
                     GRYTIVCNEPTQGAEDGGRTAMENCLSKNPGVNVVYTINEPSANGAYKALQAAGVKDA
                     LIVSVDGGCAGVKSVKDAIIGATSQQYPLLMASKGVEAIKAIATGGAKPANSAGLDFF
                     DTGVALVTDRAATGLQSIDTTKGTELCWG"
     gene            1588..2643
                     /locus_tag="VHM65_05950"
     CDS             1588..2643
                     /locus_tag="VHM65_05950"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2705281.1"
                     /translation="MAHDDEEAPMSAGAPAHSAAEEFAARQHSSSQRIQHLLHAHPAI
                     SPALVLVVATAVFGLINPRFVQPGSLSLVLQQFAVVGLLAVGQTLVILTAGIDLSVGA
                     ITILAMMLMGSLSTKNHLAGFVALVIGWAFAAAAGAFNGALVTRLSLPPFIVTLGTLS
                     IFTAVALLYSGGKSIDGSLMPDLLNVMGNGFGIGPFRFTVGVAVVLLVYALLGFVLSQ
                     TAWGRHVYATGDDIEAARLSGISVNRVLLSVYAVAGAIFGLAAWVQIGRAGAASPNGI
                     VDANLESITAVVIGGTSLFGGRGALLGTLLGALIVRVFGSGLALAGVDSQWRVFAIGV
                     LVIVAVSIDQWIRTVRA"
     gene            2640..3476
                     /locus_tag="VHM65_05955"
     CDS             2640..3476
                     /locus_tag="VHM65_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2705282.1"
                     /translation="MNPILSGRGLVKTFGRVVGLDGVDMDLFPGEVLAVIGDNGAGKS
                     TLIKCLSGAMVPDAGEITLDGAPVSFRGPQDARDAGIETVYQQLALSPALDIASNLFL
                     GRELRRPGVLGSVLRMLDHAEMRRRAGESISNLGIETVQNMGQTVETLSGGQRQAVAV
                     ARAAAFGSKVVVLDEPTAALGVKEGNQVLRMIEQLRENGLPVIIISHNMPHVYAVADR
                     IHIQRLGRCAGVITTKSHSMNQAVAIMTGAAVLDPADSVTGPVGATDGGRSSPRDPSH
                     PA"
     gene            3487..3756
                     /locus_tag="VHM65_05960"
     CDS             3487..3756
                     /locus_tag="VHM65_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705283.1"
                     /translation="MQWWQVVMAGILVVGAVRVVVLVLRLRRGLAPTPEECEMRLTPQ
                     ARAQVGSAIASGKLVLAVRHYRDATGADLQTAHEAVQRWAKGIRG"
BASE COUNT          576 a         1367 c         1246 g          594 t
ORIGIN      
        1 tcctgcgcct ggtcgctcgc aagagatcgc gctggtcggg ttcgacgatt tccccctcgc
       61 cgacctgctc gagccggccc tcaccgtcgt ccgccaggac gtccgcatcg tggcggagcg
      121 agtctgcgag ctggtcttcg cccgggtcga gggcgacacg agcccgccgc agcacgtcgt
      181 cgttgcgacg actctcgtga cccgcggctc gggtgagatc cccccgagga cctaacgcag
      241 cggcataccg cccgcatggc gcacgggtag gtctcgattc cgacacgacg gcgggtcgca
      301 cttgacgggt cgtggaccct cccttataac gttgacaacg ttgtctcacc ggacaacccc
      361 gactcgggag aggccgccca tgctcgccac cacgtcgcgc cggtctggcg ccctggccct
      421 ggccggcgtt gtcgccgccg gcctgagcct cgccgcgtgc agcagcagct cgtccacctc
      481 cagcccgtca ggggctgcgg ggggcgatgc ggtcgccgtc tccctcatca ccaaggactc
      541 gaccaacccc ttcttcgtcg ccatgcagaa gggtgccaag gaagccgccg agaagaatgg
      601 cgtcgcgctc accgtggcct cgggctccaa ggatggcgac gaggcgggcc agatcaccgc
      661 catcgagaac gcgatcgcgg ctcaggccaa gggcatcctc atcacgccca acggtcccgg
      721 tgtcaactcg gccatcaaga aggcgcgcga ggccggcctc tacgtcatcg ccctggacac
      781 gccgcctgac ccggccgaca ccgtcgacat caccttcgcg accgacaact tcctggccgg
      841 caagctcatc ggccagtggg ccaagaccca gctcgcaggc aaggacgcga cgatcgccct
      901 gctcgacctg ttcaacgaca aggtcgtctc cgtcgactac aaccgtgacc agggcttcct
      961 cgccgggatg ggtatcgaca ccaaggacgc caagaagaac ggcgacgagg acaagaccgg
     1021 ctcgtatgcc gggggcaggt acaccatcgt gtgcaacgag ccgacccagg gcgccgagga
     1081 cggcggccgc acggccatgg agaactgcct gtccaagaac cctggcgtca acgtcgtcta
     1141 caccatcaac gagccgagcg cgaacggcgc ctacaaggcg ctgcaggctg cgggagtcaa
     1201 ggacgcgctc atcgtctccg tcgacggggg ctgcgccggg gtgaagtccg tcaaggacgc
     1261 catcatcgga gccacctcgc agcagtaccc cctgctcatg gcgtccaagg gcgtcgaggc
     1321 gatcaaggcg atcgccacgg gcggggccaa gccggccaac tcggccggtc tcgacttctt
     1381 cgacaccggt gtcgcgctcg tcacggaccg ggccgccacc ggcctgcaga gcatcgacac
     1441 caccaagggc accgagctct gctggggctg atccgaccgg ctcgtagcct gggccactca
     1501 gacccccggc gcgcctgctg tagacatcgt gggcgcgccg ggacagagtg aagcgcgacc
     1561 gccatacccc aatggggtgc gagcgccatg gcccatgacg acgaggaggc gccgatgagc
     1621 gcaggggcac ccgcgcacag cgcggccgag gagttcgcgg cccggcagca ctccagcagc
     1681 cagcgcatcc agcacctgct ccacgcccac cccgcgatca gcccggcgct ggttctcgtc
     1741 gtcgcgactg cggtcttcgg cctgatcaac ccgcggttcg tgcagccggg gtcgctctcg
     1801 ctcgtcctgc agcagttcgc cgtcgtcggg ctcctggcgg tcggtcagac cctcgtcatc
     1861 ctcaccgccg gcatcgacct gtcggtcggc gccatcacga tcctggccat gatgctcatg
     1921 gggtcgctgt cgacgaagaa ccacctggcc ggcttcgtgg ccctggtcat cggctgggct
     1981 ttcgccgccg ccgccggtgc cttcaacggc gccctcgtga cccggctcag cctgccaccc
     2041 ttcatcgtca ccctcgggac gctcagtatc ttcaccgcgg tggccctgct ctactcgggc
     2101 ggcaagagca tcgacggcag cctcatgcct gacctgctca acgtcatggg caacgggttc
     2161 ggcatcggac ccttccggtt caccgtcggg gtcgcggtcg tcctgctcgt ctacgcgctg
     2221 ctgggcttcg tcctgagcca gaccgcgtgg ggacgccacg tctacgccac cggtgacgac
     2281 atcgaggcgg cccgcctgtc cggcatcagc gtcaaccggg tcctgctcag tgtgtatgcc
     2341 gtcgccgggg ccatcttcgg ccttgccgcc tgggtccaga tcggccgggc cggcgcggcg
     2401 agccccaacg gcatcgtcga tgccaacctc gagtcgatca ccgccgtggt catcggcggc
     2461 accagcctct tcggcggacg tggcgccctg ctcggcacgt tgctcggggc gctgatcgtg
     2521 cgggtcttcg ggtccgggct ggccctggcc ggcgtcgact cccagtggcg ggtcttcgcc
     2581 atcggcgtcc tggtcatcgt ggccgtctcg atcgaccagt ggatcaggac ggtgcgggca
     2641 tgaaccccat cctgtccggg cgcgggctcg tcaagacctt cggtcgcgtc gtcgggctcg
     2701 acggcgtcga catggacctg ttccccggcg aggtcctggc tgtcatcggc gacaacggtg
     2761 cgggcaagtc caccctgatc aagtgcctct ctggcgcgat ggttcccgat gccggggaga
     2821 tcaccctcga cggggcaccg gtgtcgttca ggggccccca ggacgctcgg gatgccggca
     2881 tcgagacggt ctaccagcag ctggcgctct cgcccgcgct cgacatcgcc tccaacctct
     2941 tccttggtcg tgagctgcgg cggcccggag tcctcgggtc ggtcttgcgg atgctcgacc
     3001 acgccgagat gcgcaggcgg gcgggggagt cgatctccaa cctcgggatc gagacggtgc
     3061 agaacatggg ccagaccgtc gagaccctct ccggaggcca gcgtcaggcc gtcgctgtgg
     3121 cccgcgccgc cgcgttcggc agcaaggtcg tcgtgctcga cgagccgacc gcggccctgg
     3181 gcgtcaagga aggcaaccag gtcctgcgga tgatcgagca gctccgtgag aacggcctgc
     3241 cggtcatcat catcagccac aacatgccgc acgtctacgc cgtcgccgac cggatccaca
     3301 tccagcgtct gggccgctgc gccggagtca tcaccacgaa gtcgcactcg atgaaccagg
     3361 cggtcgccat catgaccggt gccgcggtcc tggacccggc tgacagtgtc accggccccg
     3421 tcggggcgac agacggtggt cgctcttcgc cgagggaccc ttctcacccg gcgtaaggtc
     3481 gccggtatgc agtggtggca ggtcgtcatg gcggggattc tcgtcgtcgg cgccgtgcgg
     3541 gtggtcgtgc tcgtgctccg gctcagacgt ggtctcgcac cgactccgga ggagtgcgag
     3601 atgcggctga ccccccaggc ccgggcccag gtcggctccg ccattgcgag cggcaagctc
     3661 gtcctcgccg tccggcacta ccgtgacgcg accggggccg accttcagac cgcacacgag
     3721 gcggtccagc gttgggccaa gggcatccgt ggctgatcac tgctgcggca ggtcgacgag
     3781 cgc
//
LOCUS       DATAIX010000395         7320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_21302, whole genome shotgun sequence.
ACCESSION   DATAIX010000395 DATAIX010000000
VERSION     DATAIX010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21302"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3512
                     /locus_tag="VHM65_05965"
     CDS             <1..3512
                     /locus_tag="VHM65_05965"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF011498.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX2705284.1"
                     /translation="EKVIYFAAYMITWVDDESRHRDLPSLEAQIQVEKKEHENRRDVD
                     VDTRAKKLEANLAELEAEGAKADVRRKVRESAEREMNALRRRADQQIERLAQVWERFK
                     NLKVQDLEGDEVLYREMRDRFGLYFEGGMGAAAIQKRLETFDLAAESELLREIIATGR
                     GQKKTRALKRLKVVTAFLQTSNSPLGMVLDCVPVIPPDLRPMVQLDGGRFATSDLNDL
                     YRRVINRNNRLKRLLDLGAPEIIVNNEKRMLQEAVDSLFDNGRRGRPVTGPGNRPLKS
                     LSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKQMALELFKPFVMKR
                     LVDLDHAQNIKSAKRMVERAKPVVWDVLEEVITEHPVLLNRAPTLHRLGIQAFEPQLV
                     EGKAIQIHPLVCTAFNADFDGDQMAVHVPLSAEAQAEARILMLSSNNILKPADGRPVT
                     MPTQDMIIGLYHLTSEIEDGKGAGRAFSSPAEARMAFDSGQIELGSMVKIRLRDLVPT
                     GGSTLPAGAEVGADGLVSALIVETTLGRAIFNEALPVDYPFVNDSVDKKRLSTIVNDL
                     AERYSKVQVAASLDALKEAGFHWATRSGTTVSISDVVTPPRKAEILEEYETRAAKVQK
                     LYDRGAMTDNERRQDLIEIWNEATNEVAKAMIANIPPTNTIFRMVSSGARGNWMQLRQ
                     IAGMRGLVSNPKGEIIPRPIRANFREGLSVLEFFISTHGSRKGLADTALRTADSGYLT
                     RRLVDVSQDVIIREDDCGTERGLTMPIAATDPTGQRVLHDDVETSVYARTLAEDVVQG
                     SETLATAGIDLGDVVIDKLFDAGVNQVRVRSVLTCDSKVGTCARCYGRSLAAGKLVDI
                     GEAVGIIAAQSIGEPGTQLTMRTFHTGGIAGDDITQGLPRVVELFEARTPKGVAPIAE
                     ATGRVKIEDTDKTRRILLTPDDGSDEHAYPVSKRARLLVADGDHVEVGHQLVQGAIDP
                     KQVLRILGPRSVQMHLVDEVQSVYRQQGVSIHDKHIEVIVRQMLRRVTIIESGDADLL
                     PGELAERGRFEVENRRVVAEGGKPASARPELMGITKASLATESWLSAASFQETTRVLT
                     DAAIHAKSDPLLGLKENVILGKLIPAGTGLPRYRNITVEPTEEAKAAMYAMPNYDSYD
                     YATFGSGTGEAVRLDDLNLDFDR"
     gene            3676..3906
                     /locus_tag="VHM65_05970"
     CDS             3676..3906
                     /locus_tag="VHM65_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705285.1"
                     /translation="MPSVQIKDVPEQTHTVLRQRAAAAHQSLQEYLRARLIEEASQPT
                     LEEILDRAGGRAGGSVPLREAVAAVRSDRARR"
     gene            3893..4174
                     /locus_tag="VHM65_05975"
     CDS             3893..4174
                     /locus_tag="VHM65_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX2705286.1"
                     /translation="MLVVDASVLAVAIADDGPDGDQARVRLRGERLAAPELVDLEVAS
                     VLRRQLRGGAIDPRRADLALADLAALPLSRAPHRPLLTRCWELRDNLTI"
     gene            4604..4885
                     /locus_tag="VHM65_05980"
     CDS             4604..4885
                     /locus_tag="VHM65_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705287.1"
                     /translation="MLVYPDVRAAVTWLEAAFGFEESVCVGEVHRARLRVGPDGALIV
                     ADLRGRQVPSSAGWATHVLGDLAGHRWELTQTVRDVEPEQRGGVTVSPW"
     gene            complement(5787..6494)
                     /locus_tag="VHM65_05985"
     CDS             complement(5787..6494)
                     /locus_tag="VHM65_05985"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEX2705288.1"
                     /translation="MVRALLVVEHEADASLGRFARWFDAAGLGVEVVRPYGGERLPAD
                     LRGYAGLLVCGGSPAAWEDDVAPWLPQTRALLRDGVSDAIPTLGLCLGGQLLANATGG
                     QARRGSAGPEVGLGTVLPTAAAAGDPLFAHLPALGLPAAQWHVDEIAELPPKAVLLAT
                     GTPYPRQAFRLGQRAWGLQFHPEVTAAGFGSWGRTDPVPGVDMDAARASVFGMDAALE
                     AAWRPLALAFASVVCDR"
     gene            complement(6578..>7320)
                     /locus_tag="VHM65_05990"
     CDS             complement(6578..>7320)
                     /locus_tag="VHM65_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705289.1"
                     /translation="LSSLVSLIGIRGGVASVNHPFGTGGGSVLSDAAYRTKLSTTAAS
                     LLAANAYGAHVLEVGYKKRGQMSVEGHIDLWDILLSSGLRIMANGVSDNHAGNLDGWL
                     TGSNRYFTDVVSDSADPADVVPLLSSGRCFVSLRPEYSGMLDLSCDDTLMGGVRAGTG
                     STATVTATADALPSGGSLRIVQMPVHGVRNRNTATPPLWERTVTPSQLTSGAISVGVT
                     NVASYLRTEVRDADGTVVGFSNPLFLAP"
BASE COUNT         1178 a         2546 c         2506 g         1090 t
ORIGIN      
        1 tggagaaggt catctacttc gccgcctaca tgatcacctg ggtggacgac gagtcccggc
       61 accgcgacct gccctcgctc gaggcgcaga tccaggtgga gaagaaggag cacgagaacc
      121 gccgcgacgt cgacgtcgac acccgcgcca agaagctcga ggccaacctg gccgagctcg
      181 aggccgaggg cgccaaggcc gacgtgcggc gcaaggtgcg cgagtccgcc gaacgcgaga
      241 tgaacgccct gcgccgtcgg gccgaccagc agatcgagcg gctcgcgcag gtgtgggagc
      301 gtttcaagaa cctcaaggtc caggacctcg agggcgacga ggtgctctac cgggagatgc
      361 gtgaccggtt cggtctctac ttcgaaggtg gcatgggcgc tgcggcgatc cagaagcgcc
      421 tggagacctt cgacctcgcg gccgagtccg agctgctgcg cgagatcatc gccaccggcc
      481 ggggccagaa gaagacccgg gccctcaagc ggctcaaggt cgtcaccgcg ttcctgcaga
      541 cctcgaacag cccgctggga atggtcctcg actgcgtgcc ggtgatcccc ccggacctgc
      601 gtcccatggt ccagctcgac ggtggccgct tcgccacctc agacctcaac gacctctacc
      661 gccgggtcat caaccgcaac aaccgcctca agcggttgct cgacctcggg gcaccggaga
      721 tcatcgtcaa caacgagaag cggatgctcc aggaggccgt cgactcgctg ttcgacaacg
      781 gccgccgtgg acgcccggtg accggcccgg gcaaccggcc gctcaagtcg ctgtccgaca
      841 tgctcaaggg caagcagggc cggttccggc agaacctgct cggcaagcgc gtcgactact
      901 ccggccggtc ggtcatcgtc gtcggcccgc agctcaagct gcaccagtgc ggcctgccca
      961 agcagatggc gctcgagctg ttcaagccgt tcgtcatgaa gcgcttggtc gacctcgacc
     1021 acgcccagaa catcaagtcg gccaagcgga tggtcgagcg ggccaagccg gtcgtctggg
     1081 acgtcctcga agaggtcatc accgagcacc cggtcctgct caaccgggcg ccgacgctgc
     1141 accgcctcgg tatccaggcc ttcgagccgc agctcgtcga gggcaaggcc atccagatcc
     1201 acccgctcgt ctgcacggcg ttcaacgccg acttcgacgg ggaccagatg gccgtgcacg
     1261 tgccgctgtc ggccgaggcc caggccgagg cccggatcct catgctgtcg agcaacaaca
     1321 tcctcaagcc ggccgacggc cgtccggtga cgatgccgac ccaggacatg atcatcggcc
     1381 tctaccacct gacgtccgag atcgaggacg gcaagggcgc cggacgggcc ttctcgtccc
     1441 cggccgaggc ccggatggcc ttcgactcgg gccagatcga gctcggctcc atggtcaaga
     1501 tccgcctgcg cgatcttgtc cccactggcg ggagcaccct gcccgcaggc gccgaggtgg
     1561 gcgcggacgg tctcgtgtcg gccctgatcg tcgagacgac cctgggccgg gcgatcttca
     1621 acgaggccct gcccgtcgac tacccgttcg tcaacgactc ggtcgacaag aagcgtctct
     1681 cgaccatcgt caacgacctc gccgagcgct actccaaggt gcaggttgct gcctcgctgg
     1741 acgcgctcaa ggaggccggc ttccactggg cgacgcgctc ggggacgacg gtgtcgatct
     1801 ccgatgtcgt caccccgccg cgcaaggccg agatcctcga ggagtacgag acccgcgcag
     1861 ccaaggtcca gaagctctac gaccgtggcg ccatgacgga caacgagcgc cgccaggacc
     1921 tcatcgagat ctggaacgag gcgaccaacg aggtcgccaa ggcgatgatc gcgaacatcc
     1981 cgccgaccaa cacgatcttc cggatggtct cctccggggc gcgtggcaac tggatgcagc
     2041 tgcgccagat cgccgggatg cgtggcctcg tgtcgaaccc caagggtgag atcatcccgc
     2101 ggccgatccg cgcgaacttc cgcgagggtc tgtccgtgct cgagttcttc atctcgacgc
     2161 acggctcccg caagggtctg gcggacaccg cgctgcggac cgcagactcg ggctacctca
     2221 cccgtcgcct cgtcgacgtg tcgcaggacg tcatcatccg tgaggacgac tgcgggaccg
     2281 agcgtggcct caccatgccg atcgcggcga ccgaccccac gggtcagcgt gtcctgcacg
     2341 acgacgtcga gacgtcggtc tacgcccgca cgctggccga ggacgtcgtc cagggcagcg
     2401 agacgctggc tacggcgggg atcgacctcg gcgacgtcgt catcgacaag ctcttcgacg
     2461 ccggcgtcaa ccaggtgcgg gtccgctcgg tcctcacctg cgacagcaag gtcgggacct
     2521 gtgcgcgctg ctacggccgg tcgctcgcag ccggcaagct ggtcgacatc ggtgaagcgg
     2581 tcggcatcat cgccgcccag tcgatcggtg agcccggcac ccagctgacg atgcggacct
     2641 tccacaccgg tgggatcgcc ggtgacgaca tcacgcaggg tctcccgcgt gtggtcgagc
     2701 tcttcgaggc ccgcaccccc aagggtgtcg cgccgatcgc ggaagcgacc ggccgggtca
     2761 agatcgagga caccgacaag acgcgccgga tcctgctgac cccggacgac ggctcggacg
     2821 agcacgccta cccggtgagc aagcgcgccc gcctgctcgt ggccgacggt gaccacgtcg
     2881 aggtcgggca ccagctcgtc cagggcgcga tcgaccccaa gcaggtcctg cgcatcctcg
     2941 ggccacgctc ggtccagatg cacctggtcg acgaggtcca gagcgtctac cgccagcagg
     3001 gtgtgtcgat ccacgacaag cacatcgagg tcatcgtccg gcagatgctt cgccgggtga
     3061 cgatcatcga gtccggtgac gcggatctgc ttccgggcga gctcgccgag cggggacgct
     3121 tcgaggtgga gaaccgccgc gtggtcgccg agggcggcaa gccggcctcg gcccgtcccg
     3181 agctcatggg tatcaccaag gcctcgctcg cgaccgagtc gtggctgtcg gcggcgtcct
     3241 tccaggagac gacgcgggtg ctcaccgatg cggcgatcca cgccaagtcg gacccgctgc
     3301 tcggcctcaa ggagaatgtc attctcggca agctcatccc ggcagggacg ggcctgccgc
     3361 gctaccgcaa catcacggtc gagccgaccg aggaggccaa ggcggcgatg tacgcgatgc
     3421 ccaactacga ctcctacgac tacgcgacct tcggctcggg gacgggcgag gccgtccgcc
     3481 tggacgacct caacctcgac ttcgaccgct gagctcgagg attctgcacc acccaggaag
     3541 ggggacggcc gggcaccggc tgtccccgat ccggcgttct cagggggacg cgtcggtcca
     3601 ccgatgcagt cgttcagcca cctagtgtca acgcggatgg ttgtgagttg gcgcgctgag
     3661 cgccataatg gcgccatgcc cagtgtccag atcaaggatg ttccggaaca gacgcacact
     3721 gtcctgcgcc agcgcgccgc cgccgcacac cagtcgctgc aggagtatct gcgggcccgg
     3781 ttgatcgagg aagcgagcca gccgacgctg gaggagatcc ttgaccgggc cggtggtcgg
     3841 gccggcggtt cggtgccctt gagagaggca gtggcggccg tccggtccga tcgtgctcgt
     3901 cgttgacgcc agcgtcctgg ccgtcgcgat cgcggacgac ggcccggatg gcgaccaggc
     3961 ccgggtccgc ctgcgcgggg aacggctggc cgcacccgag ctggtcgacc tagaagtcgc
     4021 ctcggtcctg cgccgccagt tgcgaggcgg cgcgatagac ccccgccggg ccgacttggc
     4081 gctcgccgat ctcgcggcgc tgcccctctc gcgcgcaccg catcggccac tgttgacccg
     4141 gtgttgggaa ctgcgcgaca acctcacgat ctaggacgcc gcatacgtcg cgctcgccga
     4201 agcgctccaa acgaccttgc tcaccggcga ccggcgaatg gcacggtccg ccggaccgcg
     4261 atgtgacatc gaagtgtttc gaccgacacg ctgaacccca ttccggacgc ccggctcggc
     4321 ccgctgggag ggcggccacg ctcgcctggt catgcgcaaa gggtggggac ttcgacgtgg
     4381 ccaccagcgg ggacctcggc ttggtcattg acagcgggcg gctccgctcg tccggtcagg
     4441 gcgcctgtcg gagtgaggct ccccccaatc gtaggcttcg ctacgccacc ggacagaagc
     4501 tcagagctcg gtagccgagc atcgaggatg gcggccggcc atgctcacga cattggcgac
     4561 tgtgctgacc aatcgttcgg cgccaccgga aactgtgacg ccagtgcttg tctaccctga
     4621 cgtgcgcgcg gcggtcacct ggctggaagc cgcgttcggg tttgaggaga gtgtctgcgt
     4681 cggggaggtg caccgggctc ggctgcgcgt ggggccggat ggtgcgctga tcgtcgcgga
     4741 cctccgcggg cggcaggtcc catccagcgc cggctgggcg acgcacgtgc taggggacct
     4801 ggccggccac cgctgggagc tgacccagac cgtgcgtgac gtcgagcccg aacagcgggg
     4861 tggcgtcacc gtctcgccct ggtaacccgt cacgcgagcg aggaagccga cagcccggca
     4921 ccaacacagc tacctgcccg ccgcccttca cgtgcctcac ctccttcgac tccgccgctt
     4981 gggagggcgg ctgcgctcgc ccggtctagc cgcttccggg tcgcagccgc cgacagacgg
     5041 ctgtagcgcg ggcccagggg gtgcaggaga ctagctgcat ggtccctcgc gcaccgtccc
     5101 tcaggtccac gtgtgcgcac ttctccgttt gggccctcgt gggtgcggga tatgcactgg
     5161 gagtattgag catcctgacc atcggcgtca tggtgctcgc gatctcgatc gcggccacgg
     5221 tcatcctcgt acgaagcggg ttcgctctcg gtgggacggt tggcatggtg tcgggcgtat
     5281 cactgccctt gttctacgtg gcgtttctga atcgcggagg acccggcttg gtgtgtacct
     5341 cgagtgcagt ggaaacgtcg tgtaccgaat ctacgagccc ttggccatgg ctccttgcgg
     5401 gcatctgcgc gttggcagcc gggctcatcc tgttcgcgcg aagcggcgca cgtcgaggca
     5461 agccgtcccc tcgacggctt tccgcgtagt cgcttaggac gtccggtcat gtcgcataca
     5521 gcgcgcgttg tgcgaagttg gctgcctctc gacagcacgc tccacccatt gctcgcccgc
     5581 ctcgaggacg acggactggt cgcgagcacg aggcagccgg gcggagccgt ctcggcgggc
     5641 tcatgctcgt gttcttcctc gccgtcatgg gcacgctgcg tcgcgacgag cgccggtcgg
     5701 tcatcgaccc ccgcatggcg ccgtctggtg actccggcta tggccgggcc aaccggcagt
     5761 tggggcgcac gggcccgggc tcggacctac ctgtcgcaga ccacactcgc gaaggccagg
     5821 gcgagcggac gccaggctgc ctcgagggcg gcgtccatgc cgaagaccga cgcacgggcc
     5881 gcgtccatgt cgacgcccgg caccgggtcg gtgcgccccc acgagccgaa gccggccgcc
     5941 gtcacctcgg ggtggaactg caggccccac gctctttggc ccaggcgaaa ggcttgccgg
     6001 ggatacggcg ttccggtggc caggaggacc gccttcgggg gcagctcggc gatctcgtcg
     6061 acgtgccatt gcgcggcggg cagaccgaga gcgggaaggt gcgcgaacaa cgggtcaccg
     6121 gccgcggctg ccgtgggcag cacggtgccg aggcccacct cgggcccggc gctcccgcgt
     6181 cgagcctgcc cgccggtggc gttcgcgagc agctgaccgc cgaggcacaa ccccagcgtc
     6241 ggtatggcgt cgctgacccc atcgcgcagc agcgcccggg tctgggggag ccagggcgcg
     6301 acgtcgtcct cccaggcggc cggtgagccg ccgcacacga gcaggccggc gtagccacgc
     6361 agatcggcgg ggagccgctc accgccatac gggcgcacga cctcgacgcc gagcccggcc
     6421 gcgtcgaacc accgggcgaa ccgccccagt gacgcgtccg cctcgtgctc gacgaccaag
     6481 agggccctga ccatggcagg ccatcctagg cgctggcgcc ggaggggcgc tggcgcggta
     6541 gcccggcgct ctcgaagctg gggccgagcc gggtggacta tggagcgagg aagagggggt
     6601 tggaaaagcc cactaccgtg ccgtccgcgt cgcggacctc ggtccgcagg tagctggcga
     6661 cattcgtgac gcccaccgag atcgcgcccg acgtcagctg cgagggggtc accgtccgct
     6721 cccacagtgg cggggtcgcg gtgttccggt tccgcacccc gtggaccggc atctggacga
     6781 tccgcagact cccgcccgag ggcagggcat cggcggttgc cgtcaccgtc gcggtcgagc
     6841 cggtgcccgc tcgcactccc cccatgagtg tgtcgtcgca ggacaggtcg agcatgccgg
     6901 agtactcggg acgcaggctg acgaaacagc gacccgagga gagcaggggg acgacgtcgg
     6961 ccgggtcggc ggagtcgctg acgacgtcgg tgaagtagcg gttcgacccg gtgagccagc
     7021 cgtcgaggtt gccggcatgg ttgtcggaca caccgttggc catgatccgc agcccggagc
     7081 tcaacaggat gtcccacagg tcgatgtgcc cctcgacgct catctgcccg cgcttcttgt
     7141 acccgacctc caggacgtgc gcgccatacg cgttggcagc caggagcgat gccgcggtgg
     7201 tcgagagctt ggttcggtat gccgcgtcgc tcagcaccga accgccgccg gtcccgaagg
     7261 ggtggttgac cgaagcgacg ccgcccctga tgccgatgag gctgacgagc gagctgagat
//
LOCUS       DATAIX010000396         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_51459, whole genome shotgun sequence.
ACCESSION   DATAIX010000396 DATAIX010000000
VERSION     DATAIX010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51459"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VHM65_05995"
     CDS             <1..220
                     /locus_tag="VHM65_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705290.1"
                     /translation="SMGSYCFSVDIEGHVNDERVGESLTGLKRVCADIVFLGSYARAD
                     RGRPTIAPGNTDHDFVAAQEWLAGLRGG"
     gene            complement(243..893)
                     /locus_tag="VHM65_06000"
     CDS             complement(243..893)
                     /locus_tag="VHM65_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX2705291.1"
                     /translation="MRTRTRALVALLWTATLWAAVVGVGLLLRGRLHAVEDFEDSLSR
                     DVADDRSAALDTATALWSRLGSPALLLGVGALAVVVIRWRTLEWRVAVIHALALGLQA
                     LVVLTAGVVLRRSGPAVTRLDPAATTSSYPGSQAAAGTALCVSVALVAAFHVRHRWLR
                     AVAITVALAIPVLVAFAGVYRGTHHLSDVTMGAATGLGCAALAWRSARPADIGAGT"
     gene            complement(899..2011)
                     /locus_tag="VHM65_06005"
     CDS             complement(899..2011)
                     /locus_tag="VHM65_06005"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX2705292.1"
                     /translation="MPTPTEVWIAIAVAAVVIALVAVAVVRGRRSPGDYDPILLGLRL
                     RRRPHRDHFRPEGHGDTTAPPLKRIAVVVNPTKFEDLAAVRRSIATGCRESGWGEPIW
                     LETTQADPGTGQARLALEQGADMVCPLGGDGTVRTVAMALAGTETPLGLLPAGTGNLL
                     ARNLDLPVYDLSQALKVALLGRNKRIDVGRLTVDLSGEHERPEEHLFLVMAGFGFDAA
                     VMADAPEKLKAAVGSAAYVVSGLKNLGGQRFKVRVSVDGGPEFTRRTRTVLIGNCGKL
                     FGGLVLMPEAKVDDGILNAVIISPKGLVGWAAVLARVATQQRKGHERVDHHSGREIHV
                     RADRLEAVQVDGDTVGQARVLTAVVEPGALVVRIGA"
     gene            2085..>2596
                     /locus_tag="VHM65_06010"
     CDS             2085..>2596
                     /locus_tag="VHM65_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX2705293.1"
                     /translation="MADEPRTPADPISPDTKDWTWVLTRACADCGFDPAAVGRATVGA
                     RIEAALAPWPAVLARDGVRTRPRRGVWSPLEYACHVRDVCRIFAARAGLMREQEDPTF
                     AGWDQDATAIQERYDAQDPAVVAIEVGAASAAAVAAFAAVRAGEWDRTGVRSDGSRFT
                     LETLAAYFLH"
BASE COUNT          362 a          943 c          952 g          339 t
ORIGIN      
        1 gtcgatgggg tcgtactgct tctcggtcga catcgagggc catgtcaacg acgagcgggt
       61 cggcgaaagt ctcaccgggc tgaagcgggt ctgtgccgac atcgtcttcc tcggctccta
      121 cgcccgggcc gaccgtggcc ggccgaccat cgcccccggc aacaccgacc acgacttcgt
      181 tgctgcccag gagtggctgg cagggctgcg cggcggctga cggtggtgct cggcgggacg
      241 ggtcaggtgc cggcgccgat gtcggctggg cgcgccgacc gccacgccag ggccgcgcag
      301 ccgagtccgg tcgctgctcc catcgtgacg tcgctcaggt ggtgagtgcc ccggtagacc
      361 ccggcaaagg ccacgagcac ggggatggcg agcgccactg tgatcgctac tgcccggagc
      421 cagcggtggc ggacgtggaa ggccgccacg agcgccacgc tgacgcacag cgccgtgccc
      481 gcggcggcct gcgagcccgg gtagctcgac gtcgtggccg ccgggtccag ccgggtgacg
      541 gcgggtccgc tccgccgcag gacaaccccc gcggtgagca cgaccagtgc ctgcaggccg
      601 agggccagcg cgtgtatgac ggccacccgc cactcgagcg tgcgccagcg gatcacgacg
      661 acggcgaggg cgcctacccc gaggaggagc gccgggctgc ccagccgtga ccacagcgcg
      721 gtcgcggtgt ccagggccgc ggagcgatcg tcggccacgt ccctgctcag ggagtcctcg
      781 aagtcctcga ccgcatgcag ccggccgcgg agcagcagcc cgacgccgac gaccgcggcc
      841 cacagagtcg cagtccacag cagggccacg agggcgcggg tgcgggtccg cacgggtttc
      901 aggcgccgat ccgcacgacc agagcgcccg gctcgaccac ggcggtgagc acccgggcct
      961 ggccgacggt gtcgccgtcg acctggaccg cctcgagccg gtcggcccgg acgtggatct
     1021 cgcggccgct gtggtggtcg actcgctcat gtcccttgcg ctgctgcgtc gccaccctcg
     1081 ccaacacggc cgcccatccg accaggccct tgggcgagat gatgacggca ttgaggatgc
     1141 cgtcatcgac cttggcctca ggcatgagga cgaggccgcc gaacagcttg ccgcagttgc
     1201 cgatcagcac cgtgcgcgtg cgccgggtga actcgggccc gccgtcgacg ctgacgcgga
     1261 ccttgaaccg ctggccaccg aggttcttca ggccggagac gacgtaggcc gccgacccca
     1321 cggctgcctt gagcttctcg ggcgcgtcgg ccatcactgc ggcgtcgaaa ccgaacccgg
     1381 ccatgacgag gaacaggtgc tcctccgggc gctcgtgctc gccggagagg tcgacggtga
     1441 gccggccgac gtcgatccgc ttgttccgcc ccagcagcgc gaccttgagc gcctggctga
     1501 ggtcgtagac gggcaggtcg aggttgcggg cgagcaggtt gcccgtgcct gcggggagca
     1561 acccgagggg ggtctcggtg cctgcgagcg ccatcgccac ggtccggacg gtgccatcgc
     1621 cgccgagggg gcagaccatg tccgcgccct gctccagtgc gagcctcgcc tggcccgtgc
     1681 cggggtcggc ctgggtcgtc tccagccaga tcggctcacc ccacccggac tcgcggcatc
     1741 cggtcgcgat gctccggcgg actgccgcca ggtcctcgaa cttcgtcggg ttcaccacga
     1801 cggcgatgcg tttgagcggc ggcgcggtgg tgtcgccgtg accctcgggg cgaaagtggt
     1861 cgcggtgcgg ccgccgccgc aaccgcagcc ccagcaggat cgggtcgtag tcgccgggag
     1921 agcggcgccc gcgcaccacg gccacggcga ccagggcgat gacgacggcc gcaaccgcga
     1981 tcgcgatcca gacctcggtc ggcgttggca cgagggtgac gctaccggag gatgggcacc
     2041 cgaaaggacc ggcaccggta atggtccggt actagcctgg cgcgatggcc gatgaaccca
     2101 ggaccccagc cgacccgatc agtcccgata cgaaggactg gacgtgggtg ctcaccaggg
     2161 cctgcgccga ctgcggcttc gacccggccg ccgtcggccg ggccactgtc ggcgcccgca
     2221 tcgaggcagc cctggcgccg tggcccgccg tcctcgcccg cgacggcgtg cgcacccgcc
     2281 cgcggcgtgg ggtctggtcg ccactggagt atgcgtgtca cgtccgcgac gtctgccgca
     2341 tcttcgcggc ccgggccggg ctcatgcgcg agcaggagga ccccaccttc gccggctggg
     2401 accaggacgc gaccgccata caggagcggt atgacgccca ggaccccgcc gtcgtcgcga
     2461 tcgaggtcgg cgcggcgtcg gccgcggcgg tggccgcctt cgccgcggtg cgggcgggcg
     2521 aatgggaccg caccggggtc cgcagcgacg gctcccggtt cacgctcgag accctcgcgg
     2581 cgtacttcct gcacga
//
LOCUS       DATAIX010000397         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_170857, whole genome shotgun sequence.
ACCESSION   DATAIX010000397 DATAIX010000000
VERSION     DATAIX010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170857"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VHM65_06015"
     CDS             complement(<1..509)
                     /locus_tag="VHM65_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HEX2705294.1"
                     /translation="MTDVDFTEVWRQTLSHLEADGVPSQARALLQLSRLVGILDHTAL
                     LSVPNDYTKDYIELRSREVVAAALGVRMGRAIRIAVSVDPALESHPPLTPLGSVAVPD
                     VARPDLVGSAPEGLASGRAVGHDEVEDSGPARRRQRDTPMDAEKTRLNPKYTFDTFVI
                     GASNRFAHAA"
     gene            1091..1228
                     /gene="rpmH"
                     /locus_tag="VHM65_06020"
     CDS             1091..1228
                     /gene="rpmH"
                     /locus_tag="VHM65_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX2705295.1"
                     /translation="MSKRTFQPNTRRRAKTHGFRLRMRTRAGRAILAARRRKGRAELS
                     A"
     gene            1237..>1581
                     /gene="rnpA"
                     /locus_tag="VHM65_06025"
     CDS             1237..>1581
                     /gene="rnpA"
                     /locus_tag="VHM65_06025"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX2705296.1"
                     /translation="MLPARHRLRSSADFAAAVRHPGGVRSADRLMVVHLRPSDGSGVT
                     GGSGVTGGSGMTAPRVGFVVAKSVGGAVVRNRVKRRLRAQCAPLVSTLPAGSDLVVRA
                     LPAAAQASSASLH"
BASE COUNT          204 a          576 c          560 g          241 t
ORIGIN      
        1 gccgcgtggg cgaagcggtt gctggccccg atgacgaagg tgtcgaacgt gtacttcggg
       61 ttgagccggg tcttctccgc gtccatcggc gtgtcccgct ggcggcgccg tgcgggaccg
      121 ctgtcctcga cctcgtcgtg accgaccgcg cgtcccgagg ccaggccctc gggcgccgag
      181 ccgacgaggt ccggccgggc cacgtccggg acggccaccg agccgagggg tgtcagcggt
      241 gggtgcgact ccagcgcggg gtcgacggac acggcgatcc ggatcgcgcg ccccatccgg
      301 acaccgagcg ccgctgcgac gacttcgcgc gagcggagct cgatgtagtc cttggtgtag
      361 tcgttgggca ccgacagcag cgcggtatgg tccaggatgc cgacgagccg ggacagctgg
      421 agcagcgccc gcgcctgcga cggaaccccg tcggcctcga ggtgggacag cgtctgtcgc
      481 catacctcgg tgaagtccac atccgtcacg ctgaccccct ccccggcctc acgccgtcag
      541 cgcgcatcct gcacccctgc tctggccgcg gatgcggcct ggggcctcgt gtcgcgagtc
      601 ccgcgggagc cgttccacct cgacagccac agaaatgtgg acggatcaga cgtggcccgg
      661 acgtcgccgc catggtggtc tcaacgcccg ttgacgctaa caaggtgagg gccgcacggc
      721 aagcctcgac ggtgcgacgg tgcgcaaagg tctgccggat cggcgtgtcg ccgcgtgggc
      781 ggcacgcgcg gaagggataa tccggggccc ttccccagtc actccttcgc ccggcccgac
      841 gcgcatggtt tgacccctat ctccggctcc cgtaccgtgg tcgacggcct gtgcgaccgg
      901 ccgttttcgc atgctcgtga tcacctcagc tcgctcggct ggcatgacgc gggaatccga
      961 ctgcggccgg ccacctcggt cgcccgggcc agcacgccgg gaggctggga caacaccgac
     1021 tggacgtcga tgaacgcccc tgtgagacgc accatgaccg aggtcatggg tttgaccaac
     1081 ggagaccctc gtgagtaagc gcaccttcca gcccaacacc cgccgccggg ccaagaccca
     1141 cggcttccgc ctgcgcatgc gcacgcgggc cgggcgcgcc atccttgcgg ctcggcgccg
     1201 caagggccgc gccgagctgt ctgcctgagt ccccgcgtgc tgccggcacg tcatcggctg
     1261 cgctcgtccg ccgacttcgc ggcagccgtc cgtcaccctg gtggggtccg ttccgctgac
     1321 cggctcatgg tggtgcacct gcgcccgagc gacgggtccg gggtcaccgg cgggtccggg
     1381 gtcaccggcg ggtccggtat gacggcgccg cgggtggggt tcgtcgtcgc caagtcggtc
     1441 gggggtgcag tcgtgcgcaa ccgggtcaag cgccgcctgc gcgcccagtg cgcccctttg
     1501 gtgtcgacgc tgccggccgg atccgacctc gtggtgcgtg ccctgcccgc ggcggcgcag
     1561 gcgtcgtccg cctccttgca c
//
LOCUS       DATAIX010000398         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_170967, whole genome shotgun sequence.
ACCESSION   DATAIX010000398 DATAIX010000000
VERSION     DATAIX010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170967"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1479)
                     /gene="hisS"
                     /locus_tag="VHM65_06030"
     CDS             complement(136..1479)
                     /gene="hisS"
                     /locus_tag="VHM65_06030"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX2705297.1"
                     /translation="MSANAKVRPISGFLEFLPAERIIEQHFLDVIRSTFELHGFSSIE
                     TRAVEPIERLSSQGADTDKEIYAVSRLAGGDEEREARLGLHFDLTVPLARYVVENAGR
                     LTFPFRRYQIQRAWRGERPQEGRYREFTQADIDVVDVGELSAHFEAEMPLVIADAFSR
                     LPVGEYVIEVNNRKIADGFYRGIGLGDVAGTLRIVDKLDKIGPEAVAGLLVAAGATAE
                     QAQQCLDLAQISSPDLSFVERVRALGVSHPTLDEGLDALVAVIALASVHAPGRVVADL
                     RIARGLDYYTGTVYETRLVGYESWGSFCSGGRYDELATDGRTTYPGVGISIGVSRLLG
                     LLIGTGLVTASRSTPACVLVAVTDEASRAGSTAVATALRARGIACEVAPSAARFGKQI
                     RYAERRGIPFVWFLAGPEQTSDSVKDIRSGVQVDADASSWAPPAQDLRPTVTRVG"
     gene            complement(1476..2180)
                     /locus_tag="VHM65_06035"
     CDS             complement(1476..2180)
                     /locus_tag="VHM65_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2705298.1"
                     /translation="MLTIAFPAAAFDTNCYILAPSTGQECLIIDPGIGVAGTVADVLR
                     EHRLRPAAVLLTHGHLDHVYAVTPVCAAGGVAAYIHRDDRYRLVDPIRTLDPSLVGML
                     EAQFGRPASWTEPDEVVEVTGGVTLTLAGLEVSVQHAPGHTEGSVIFGLPLVPDGISA
                     QVGVVSTCISGDVLFAGSIGRTDLPGGSDRDMTRSLRDVILPLPDTTLVLPGHGPATT
                     MKRERATNPFLQGLGA"
     gene            2278..3126
                     /locus_tag="VHM65_06040"
     CDS             2278..3126
                     /locus_tag="VHM65_06040"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX2705299.1"
                     /translation="MRTEPTARAFEEQDVTRAQDRARERRRAEKLAGKIAARQAEARR
                     NRKAVALVVLVLAVVVGFVWLTSALTKKSATPTAGETTAAETTAAVLAGCTQPPAKQA
                     TPKTFSSAPDFTVVQGKTYTGTVTTSCGKIGVELYGAKAPKAVASFAQLAKAGYWAPS
                     PCHRLTTSGLFVLQCGDPTGTGSGPGPGYTFGVENAPADGKYPRATLAMARTSDPDSN
                     GGQFFIVYKDTDLPTDGGGYTIFGTVTSGLDIVERIAAAGVNVQDQTSPKAPVSILSV
                     DARAKA"
BASE COUNT          485 a         1098 c         1128 g          422 t
ORIGIN      
        1 gggtggcacg gcaccattga tatccggcag cagcgggtcc ggtggcgtcg cgggccgcta
       61 cgtcttgccc atccgcaact gaaccatacc gcgtggcggg gccgctccag gtccggtcct
      121 accacgcggt ctacgtcagc cgactcgggt gacggtcggg cgaaggtcct gcgccggggg
      181 cgcccaggac gacgcgtcgg cgtcgacctg gacgcccgag cggatgtcct tgaccgagtc
      241 gctggtctgc tccggacctg ccaggaacca gacgaacggg atcccgcggc gctcggcata
      301 ccggatctgc ttgccgaacc gcgcggcgga cggtgccacc tcgcaggcga tcccgcgcgc
      361 gcgcagcgcg gtggcgacgg cagtcgaccc cgcccgggat gcctcgtccg tcacggcgac
      421 gaggacgcac gccggggtcg agcggctcgc ggtgacgagc cccgtgccga tgagcaggcc
      481 gagcaggcgg gacacgccga tcgagatccc tacgcccgga taggtcgtgc gcccgtcggt
      541 ggcaagctcg tcatagcgcc cgccggagca gaacgacccc cacgactcgt agccgacgag
      601 ccgggtctca tagaccgtcc cggtgtagta gtcgaggccg cgcgcgatgc gcaggtccgc
      661 gacgacccgt cccggagcgt ggaccgaggc gagcgcgatg accgcgacga gcgcgtcgag
      721 accctcgtcc agcgtggggt gcgagacgcc gagcgccctg acgcgctcga cgaaggacag
      781 gtccgggctg ctgatctggg ccaggtcgag acactgctga gcctgctccg ccgtggcacc
      841 tgcggccacg agcaggcccg ccacggcctc ggggccgatc ttgtcgagct tgtcgacgat
      901 ccgcagcgtc ccggccacgt cgccgaggcc gatcccccgg tagaagccgt cggcgatctt
      961 gcggttgttg acctcgatga cgtactcacc gaccggcagc cgggagaagg cgtcggcgat
     1021 gacgaggggc atctcggcct cgaaatgcgc cgacagctcc ccgacgtcga cgacgtcgat
     1081 gtcggcctgg gtgaactcgc ggtagcgccc ctcctgcgga cgctcccccc gccaggcccg
     1141 ctggatctgg tagcgacgga aggggaaggt cagcctgccg gcgttctcga cgacataccg
     1201 ggccaggggg acggtgaggt cgaagtgcag cccgagccgg gcctcgcgct cctcgtcgcc
     1261 ccccgccagc cgtgacacgg cgtagatctc cttgtcggtg tccgcgccct ggctcgacag
     1321 ccgctcgatc ggctcgacgg cgcgggtctc gatcgaggag aacccgtgca gctcgaaggt
     1381 cgagcggatg acgtcgagga agtgctgctc gatgatccgt tcggccggga ggaactcgag
     1441 gaacccgctg atcggccgga ccttggcgtt cgcgctcacg cgcccaatcc ttgcaggaag
     1501 gggttggtgg ctcgctcccg tttcatcgtc gtcgccggcc cgtgcccggg cagaaccagt
     1561 gtggtgtccg gcagcgggag tatgacgtcg cgcagcgagc gggtcatgtc ccggtccgac
     1621 cctcccggca ggtcggtgcg cccgatcgac ccggcgaaga gcacgtcgcc gctgatgcag
     1681 gtcgagacca ccccgacctg ggcgctgatc ccgtcgggca ccaagggcag cccgaagatg
     1741 accgagccct cggtgtggcc gggggcgtgc tggacgctga cctcgagccc ggcgagggtg
     1801 agcgtcaccc caccggtgac ctcgacgacc tcgtccggct cggtccagga ggccggccgg
     1861 ccgaactggg cctcgagcat cccgaccagg gaggggtcga gggtccggat cgggtcgacg
     1921 aggcggtagc ggtcgtcgcg gtggatgtat gccgccactc ccccggccgc gcagacgggc
     1981 gtgacggcat acacatggtc gaggtgaccg tgggtgagca ggaccgcggc ggggcgcagg
     2041 cggtgctcgc gcaggacgtc ggcgacggtg cccgcgaccc cgatcccggg gtcgatgatg
     2101 aggcactcct gcccggtcga gggggccagg atgtagcagt tggtgtcgaa ggccgcggcg
     2161 gggaaggcga tcgtgagcac ccgatgaggg tatccgccgg aaccggccgt cccgtccttg
     2221 cccccgtgat cgcgtggtgg gggcacgggg ccggggccac gttccctaga ctggccggtg
     2281 cgcaccgaac ccacggcgcg agcattcgag gagcaggacg tgaccagagc gcaggaccgg
     2341 gcccgcgagc ggcgccgggc ggagaagctc gcgggcaaga tcgcggcccg ccaagccgag
     2401 gcccggcgca accgcaaggc cgtcgcgctg gtcgtgctcg tcctcgcggt cgtcgtcggg
     2461 ttcgtgtggc tcaccagtgc tctgaccaag aagtcggcca ccccgaccgc cggggagacg
     2521 accgccgccg agactaccgc cgcggtcctg gcgggctgca cccagccgcc ggccaagcag
     2581 gccaccccca agaccttcag cagcgcaccc gacttcaccg tggtgcaggg caagacctat
     2641 acggggacgg tcacgaccag ctgcggcaag atcggcgtcg agctgtatgg cgccaaggcc
     2701 cccaaggcgg tcgcgtcctt cgcccagctg gcgaaagcag gctactgggc gcccagtccg
     2761 tgccaccggc tcacgacgag tggcctgttc gtcctgcagt gcggtgaccc cacgggcacg
     2821 ggctctggac ccggtccggg ctacaccttc ggcgtggaga acgccccggc cgacggcaag
     2881 tacccccgag ccacgctggc catggctcgc accagcgacc ctgacagcaa cggcggccag
     2941 ttcttcatcg tctacaagga caccgacctg cccacggacg gcggcggcta cacgatcttc
     3001 ggcacggtca cctctggact cgatatcgtc gagcggatcg ccgctgccgg ggtcaacgtg
     3061 caggaccaga cgtcccccaa agccccggtc agcattctgt ctgtcgacgc ccgagcgaag
     3121 gcctgaccgt gtc
//
LOCUS       DATAIX010000399         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_111340, whole genome shotgun sequence.
ACCESSION   DATAIX010000399 DATAIX010000000
VERSION     DATAIX010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111340"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VHM65_06045"
     CDS             complement(<1..508)
                     /locus_tag="VHM65_06045"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX2705300.1"
                     /translation="MSVDPPAGRELDLVLFGATGFVGELIAEHLVETAPDGVRVGLAG
                     RSQARLRDLRVDLGPRASDWPLIVADASEGDSLVALAARARVVASAVGPYAKYGMALV
                     EACAAAGTHYCDLTGEVLFVRRSIDAYDALAKVSGARIVHACGFDSIPSDLGVFATAA
                     RARADGQGE"
     gene            complement(628..1278)
                     /locus_tag="VHM65_06050"
     CDS             complement(628..1278)
                     /locus_tag="VHM65_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX2705301.1"
                     /translation="MTDRTYTDITMVLDRSGSMSSLRDATIEGFNAFLAEQRATPGRC
                     TVSLVQFDHEYESVYTALDLAVVPPLRLEPRGTTALLDAIGRAIHATGTRLAVMPEHE
                     RPGSVIVGIMTDGLENASRELTHAAVKALITAQTERYSWTFLYLGANQDAIEVGTGLG
                     VDAGLALTYGTGPARDALFATGANVARMRAAVAHGAAPAAARAAHAYTNEQRESVE"
     gene            complement(1339..1725)
                     /locus_tag="VHM65_06055"
     CDS             complement(1339..1725)
                     /locus_tag="VHM65_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705302.1"
                     /translation="MRDTPEQNPLTDTLAALRAELDEVEPTAPDEAVHHLSRARTLLD
                     TALDEAMALATLSGASVRSVALTAGVAPNTVAPRLARTAALGAYQAAGRVGSAGIERA
                     RHDQEEGRYAPAPQPGEPLRFRRRQD"
     gene            1952..>2153
                     /locus_tag="VHM65_06060"
     CDS             1952..>2153
                     /locus_tag="VHM65_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705303.1"
                     /translation="MTADDVAAEFDRQCAHLVGLGYPALAGIVAAELTHLLQALRSAT
                     IAALAEVDMVDPVGSVDPVGSVD"
BASE COUNT          286 a          754 c          790 g          323 t
ORIGIN      
        1 gctcgccctg accgtcggcg cgcgcccggg cagcggtggc gaagacgccg aggtcggacg
       61 ggatcgagtc gaacccgcag gcgtggacga tgcgggcccc gctgaccttg gcgagggcgt
      121 catacgcgtc gatgctgcgc cggacgaaga gcacctcgcc ggtgaggtcg cagtagtggg
      181 tgccggcggc agcgcacgcc tcgaccagcg ccatgccgta cttggcgtag gggccgacgg
      241 cgctcgccac gacccgggcc cgcgctgcca gagccacaag cgagtccccc tccgaggcgt
      301 ccgccacgat gagcggccag tcgctggcgc gcgggccgag gtcgacccgc aggtcgcgca
      361 gccgcgcctg cgagcgaccg gccagcccga cccggactcc gtcaggtgcc gtctcaacga
      421 gatgctcggc gatgagctcg ccgacgaacc cagtcgcccc gaacaggacg aggtccagct
      481 cgcggccggc aggcgggtca acgctcatgg gcgtgaccct acgcctgcca ggcgcgacac
      541 cggttctcag cgcgggccat gtgcgccgag tcgaggttct tcgtggcgga tcgccggcgc
      601 cgggtacctc gacccggcgt ggggttctta ctcgacggac tcgcgctgct cgttggtgta
      661 cgcgtgagcc gccctggccg cggcgggcgc tgccccatgg gccaccgcag cgcgcatccg
      721 ggccacattg gcgcccgtgg cgaacagcgc gtcacgggcg gggccggtgc cgtaggtgag
      781 cgccagccct gcgtcgacgc ccaggccggt gccgacttcg atggcatcct ggttggcccc
      841 gaggtagagg aacgtccacg agtacctctc ggtctgcgcg gtgatgaggg ccttgaccgc
      901 cgcatgggtg agttcgcggg aggcgttctc cagaccgtcg gtcatgatgc cgacgatgac
      961 gctccccggc cgctcgtgct cgggcatcac ggcgagccgg gtcccggtcg cgtggatcgc
     1021 ccgcccgatg gcgtcgagca gcgcggtcgt cccgcggggt tcgagccgta agggcggcac
     1081 cacggcgagg tcgagcgccg tgtagacgga ctcgtactcg tggtcgaact ggacgaggga
     1141 cacggtgcag cgtcccgggg tggctcgctg ctcggcgagg aaggcgttga agccctcgat
     1201 cgtcgcgtcg cgcagcgagc tcatcgagcc ggagcggtcg agcaccatcg tgatgtcggt
     1261 gtatgtgcgg tcggtcatgg cgtcctcctg cggtcggccg gagccccgcc gcgccagcgc
     1321 ggccgtcacc ccggtaggtc agtcctgtct gcggcggaac cgcagcggtt cccccggctg
     1381 tggcgccggc gcgtagcggc cctcctcctg gtcgtgccgt gccctctcga tgcctgcgct
     1441 ccccacccgt ccagcggcct ggtaggcacc gagggccgcc gtccgcgcca gccgcggtgc
     1501 caccgtgttg ggtgccaccc cggcggtcag ggcgaccgat ctcaccgacg ctccggagag
     1561 cgtcgcgagg gccatcgcct cgtccagtgc cgtgtcgagc agtgtcctgg cccggctcag
     1621 gtggtggacg gcctcgtcag gggccgtcgg ctccacctcg tcgagctcgg ctcgtaacgc
     1681 cgccagggtg tcggtcaggg ggttctgctc gggtgtgtcg cgcacggtcc cagcgtagtg
     1741 cagtagatgc acatgcgcaa taactgcacc agggcattaa ctgcactgaa gcaataactg
     1801 cgttcaggca ataactgcgc acagccgcgt atggcgcgcc acgagcctcg cgcggaggtc
     1861 gccctcggcc gagggcccct gtggtcgtca aggaacacgc accgtcggca cggcgttggg
     1921 tgccgtgacg gaccgagcag agggagtacg tgtgactgct gatgacgtgg ccgccgagtt
     1981 cgaccgccag tgtgctcacc tcgtcggtct gggctacccc gcgctggccg ggatcgtcgc
     2041 cgccgagctg acccacctcc tgcaggccct gcgctccgcg acgatcgccg ctctcgccga
     2101 ggtggacatg gtcgacccgg tgggctccgt ggatccggtg ggctcggtgg acc
//
LOCUS       DATAIX010000400         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_81898, whole genome shotgun sequence.
ACCESSION   DATAIX010000400 DATAIX010000000
VERSION     DATAIX010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81898"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VHM65_06065"
     CDS             <1..318
                     /locus_tag="VHM65_06065"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX2705304.1"
                     /translation="TWVPYQEDHGQGKDRPVLLVGRDGPWLLALPLTSQDHNHDAAQE
                     HRDGRDWVDIGSGPWDRRGRPSEIRVNRVVRVAETAVRREGSVLPRALFDQVSEVVRA
                     HRG"
     gene            complement(425..688)
                     /gene="rpsT"
                     /locus_tag="VHM65_06070"
     CDS             complement(425..688)
                     /gene="rpsT"
                     /locus_tag="VHM65_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX2705305.1"
                     /translation="MANIKSQIKRIKTNAKRTERNKAVKSELRTWVRKFREAAAAGDA
                     DAARSALTAASVKLDKAVSKGVIHQNQAANKKSAMARKMASLS"
     gene            complement(880..1317)
                     /locus_tag="VHM65_06075"
     CDS             complement(880..1317)
                     /locus_tag="VHM65_06075"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX2705306.1"
                     /translation="MSIEGDYAPSPTGWVRRQVEQIDAAGDTRAVSIGESEVVLLTMV
                     GRKTGQVRKVPLIRVHHEGRYVAVGSRGGAPANPEWVANLRANPELVLMDGNESTPMR
                     ARELSGAERQQWWQRAVGTFAPYAEYQSKTERLIPLFALEPSP"
     gene            complement(1349..>1731)
                     /locus_tag="VHM65_06080"
     CDS             complement(1349..>1731)
                     /locus_tag="VHM65_06080"
                     /inference="COORDINATES: protein motif:HMM:NF044544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705307.1"
                     /translation="FRVADAALAGQTAEALRLLRHAISTGVDPVPIVAVLASQLRILV
                     RVGSAGRGSSSLPVATSLGLAPWQFDKARRLVGHWEPEALATSIQAIAAADAEVKGGG
                     RDPVYAVERAILTMGAARVAGRHH"
BASE COUNT          238 a          604 c          629 g          260 t
ORIGIN      
        1 acctgggtgc cgtaccagga ggaccacggc cagggcaagg accgccccgt cctgctcgtc
       61 gggcgcgacg gcccgtggct cctggcgctc ccgctgacga gccaggacca caaccacgac
      121 gctgcgcagg agcaccggga cggccgcgac tgggtcgaca tcggctcggg cccgtgggac
      181 cggcgcggac gaccgagcga gatccgggtc aaccgggtcg tccgcgtcgc cgagacggcc
      241 gtccgacgcg aaggatcggt cttgcccagg gccctcttcg accaggtcag cgaggtcgtg
      301 cgggcccacc gcggctgacc gggacccccc gccgcttccg gtgagatgcc ccaatgggtt
      361 gtcagtcccg cctggaggca accggttcag gcatctcgtg cggcgcggtg gagggcgcgt
      421 gccctcagga gagcgaggcc atcttcctgg ccatcgccga cttcttgttg gcggcctggt
      481 tctggtggat gacgcccttg ctcacggcct tgtcgagctt gaccgaggcc gccgtgaggg
      541 cgctccgggc cgcgtcggcg tccccggcgg cggccgcctc gcggaacttg cggacccagg
      601 tgcgaagctc gctcttgacc gccttgttgc gctcggtgcg cttggcgttg gtcttgatgc
      661 gcttgatctg ggacttgatg ttcgccacgt gtgatctttc ctatggtcgg tcgggtatgc
      721 cgtgagcggg cagcagacgc ggctcgggac cgggtgtggg caccccgtgc gtgagctgcg
      781 tcagatggtg ccgatggagc tgcatacgcg cacgaccttg cgcgcacgca gtccgcaaac
      841 ctaccagggt gcgacagtgc cgaccaaaac gccgcccgcc tacggactcg gctcgagcgc
      901 gaagagcggg atcagccgct cggtcttgct ctgatactcc gcgtatggcg cgaacgtccc
      961 caccgcgcgc tgccaccact gctgacgctc ggcgccggag agctcgcggg cgcgcatcgg
     1021 ggtggactcg ttgccgtcca tgaggacgag ctccgggttg gcccgcaggt tggcgaccca
     1081 ctcggggttg gccggggcac cgccccggct gccgacggcg acatacctgc cctcgtggtg
     1141 gacccggatg agcgggacct tgcgcacctg gccggtcttg cgaccgacca tggtgaggag
     1201 caccacctcc gactcgccga tgctcaccgc ccgcgtgtcg ccggcggcgt cgatctgctc
     1261 gacctgccgg cgcacccaac cggtggggct cggtgcgtag tcgccctcga tgctcatggc
     1321 actcagtgaa gcacgggtgc gagcgtggct agtgatgacg acctgcgacg cgggcggccc
     1381 ccatcgtgag gatggcgcgc tcgacggcgt agacggggtc gcggccaccg cccttgacct
     1441 cggcatcggc cgcggcgatg gcctggatcg aggtcgcgag cgcctcgggc tcccagtggc
     1501 cgaccagacg ccgggccttg tcgaactgcc agggcgccaa ccccaaggac gtggcaacgg
     1561 gcagcgagct gctcccgcgc ccggccgagc cgaccctgac caggatgcgg agctggctgg
     1621 cgagcacggc cactatcggg acggggtcga ccccggtgga gatggcatgg cgcagcagcc
     1681 gcagggcctc ggcggtctgc ccggcaagcg cggcatcggc cacccggaag c
//
LOCUS       DATAIX010000401         4175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_82009, whole genome shotgun sequence.
ACCESSION   DATAIX010000401 DATAIX010000000
VERSION     DATAIX010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82009"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VHM65_06085"
     CDS             <1..879
                     /locus_tag="VHM65_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705308.1"
                     /translation="PQNAFGFWDWVGGRYSVDSAIGTSIAIAIGPERFAELLAGFHAI
                     DEHFRTTPLATNVPALMGLLNVWYVNFLDARTHAVLPYAQDLHRFPAYLQQLTMESNG
                     KSVRWDGTPVTTATGEVFWGEPGTNGQHAFYQLIHQGTQLVPADFIAVANPAHPLRDG
                     DADVHGLFLANFFAQTAALAFGKTAEQVRAEGTPEHIVPARVFAGNRPTTSIMAPALT
                     PSVLGQLIALYEHITFTQGVVWGVDSFDQWGVELGKQLAKALEPAVAGDESALATQDG
                     STQALIRHYLAHRHPA"
     gene            complement(901..1674)
                     /gene="pgl"
                     /locus_tag="VHM65_06090"
     CDS             complement(901..1674)
                     /gene="pgl"
                     /locus_tag="VHM65_06090"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX2705309.1"
                     /translation="MTHGPLVVVHRDQQLLADAASSRFVTAVVDAQAARGEADVVLTG
                     GTMGSAILASVGTSRIRPAIDWPRLNVWWGDERFLPSGHADRNETQARAALLDAVPLD
                     PARVHAIPGPDLSASVEAAAGAYAGTLARAARPGRDVPDFDVVLLGVGPDAHIASLFP
                     EHPALHEATASTVGVHGAPKPPPLRVSLTLRALRSGRDVWFLVSGADKASAVGLALSG
                     AGPMQAPAGAVQGTRSTVWLLDRAAATHVPPALVRISSP"
     gene            complement(1671..2819)
                     /gene="opcA"
                     /locus_tag="VHM65_06095"
     CDS             complement(1671..2819)
                     /gene="opcA"
                     /locus_tag="VHM65_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEX2705310.1"
                     /translation="MIINLPATTSAAVSKKLVTVRNDVGAMALGRVLTLIIVVDDRHA
                     DEAIEIAAEASRQHPSRIVTVVLGDKRGASRLDAQIRLGGDAGASEVVVLRLFGELAK
                     HGQSVVVPLLLPDSPVVAWWPHTAPADLSGDPIGRMAQRRITDAAAAASPRSALRARA
                     RCYHPGDTDLAWTRITRWRGLLAAALDQPPFEPIIRAVVAGAPDSPSTDLLAGWLAHF
                     LECPVKIVRTPGGSGISSVRLERTSGAIDLVRPGDTVATLSQQGQPDRRISLARRHMP
                     ECLADELRRLDPDEIYQAALLQGIEVVTHSRTTASQAVAAGESPSVAKAGALVRKLAR
                     DPAAKEAMAMVTAPAPQGSSEPVAIAAAQERKAADLARGTTATRSRRP"
     gene            complement(2816..>4175)
                     /gene="zwf"
                     /locus_tag="VHM65_06100"
     CDS             complement(2816..>4175)
                     /gene="zwf"
                     /locus_tag="VHM65_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009994655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX2705311.1"
                     /translation="HGTGFARRDWADQDFGQVVHDAVRQYARTPFREDVWRNLAEGFR
                     FVPGAFDDDSAFDLLAKTVHRLEEERGTGGNQAFYLSIPPGFFAQVCQQLERCGLSRP
                     DPKSWRRVIIEKPFGHDLGSARELNAIVEGVFPPDAVFRIDHYLGKETVQNMMALRFA
                     NQMFEPVWNANYVDHVQITMAEDIGIAGRAGYYDGIGAARDVIQNHLLQLLALVAMEE
                     PVSFGPRDLRAEKEKVLSAVRLPKDLGTASARGQYAAGWQGGEPVRGYLGEDGVSTAS
                     ATETYAAVRLDIDTRRWAGVPFYLRTGKRLGKRVTEVAVVFKRAPHLPFNDTATEELG
                     QNAIVIRIQPDEGVTIRFGSKVPGAQMEVRDVTMDFGYGSAFTETSPEAYERLILDVL
                     LGDPPLFPRHEEVELSWTILDPLITSWARSGTPEPYASGGWGPPSADDMMRRDGRHWR
                     MP"
BASE COUNT          572 a         1483 c         1526 g          594 t
ORIGIN      
        1 ccgcagaacg cgttcgggtt ctgggactgg gtgggcgggc gctactcggt cgactcggcc
       61 atcgggacga gcatcgcgat cgccatcgga ccggagcgct tcgccgagct gctggccggg
      121 ttccacgcca tcgacgagca cttccgcacg acgcccctgg ccaccaacgt gccggccctc
      181 atgggcctgc tcaacgtgtg gtacgtcaac ttcctcgatg cccggacgca tgcggtgctg
      241 ccctacgcgc aggacctgca ccgcttcccg gcatacctgc agcagctcac catggagtcc
      301 aacggcaagt cggtgcgctg ggacgggacc ccggtgacga cggcgaccgg cgaggtcttc
      361 tggggcgagc ccggcaccaa cggccagcac gccttctacc agctgatcca ccagggcacc
      421 cagctggtcc cggccgactt catcgcggtg gccaacccgg cgcacccgct gcgcgacggc
      481 gacgccgacg tccacgggct gttcctggcc aacttcttcg cccagaccgc agccctggcc
      541 ttcggcaaga cggccgagca ggtccgggcg gaggggaccc cggagcacat cgtcccggcc
      601 cgggtgttcg ccggcaaccg cccgacgacc tcgatcatgg cgcccgccct cactccgtcc
      661 gtgctcggcc agctcatcgc gctctacgag cacatcacct tcacccaggg cgtcgtctgg
      721 ggagtcgaca gcttcgacca gtggggtgtc gagctcggca agcagctcgc caaggccctc
      781 gagccggccg ttgcgggcga cgagtcggcc ctcgcgaccc aggacggctc gacccaggcc
      841 ctgatccggc actacctcgc ccaccgtcac cctgcgtagg cggcgccatc cctgcccggc
      901 tcagggtgag ctgatccgca ccagagcggg cggcacatgg gtggccgcgg cccggtcgag
      961 gagccacacc gtggagcgcg tcccctggac ggcgcccgcg ggggcctgca tcgggcctgc
     1021 cccggacagg gccagtccga cggccgaggc cttgtcggca cccgacacga ggaaccacac
     1081 gtcgcgccct gaccgcaggg cccgcagggt cagcgagacg cgcaacggcg gcggcttggg
     1141 ggccccgtgc acaccgacgg tgctcgccgt cgcctcgtgc agcgcgggat gctcggggaa
     1201 gagcgacgcg atgtgagcgt ccggcccgac cccgagcagg acgacgtcga agtccggcac
     1261 gtcgcgtcca ggccgggccg cgcgggcgag ggtgccggca tacgcgccgg cggccgcctc
     1321 gacgctggcc gagaggtctg gcccggggat ggcatggacc cgcgcggggt cgagcgggac
     1381 ggcgtcgagc agggccgccc tggcctgggt ctcgttgcgg tcggcgtgcc ccgaagggag
     1441 gaaccgctca tcgccccacc acacgttgag ccgtggccag tcgatcgccg ggcggatccg
     1501 gctcgtcccg accgaggcga ggatcgccga gcccatcgtc ccgccagtga gcacgacatc
     1561 ggcctcaccg cgtgccgcct gggcgtcgac gacggccgtg acgaagcgtg aggacgccgc
     1621 gtcggccagc agctgctggt cgcgatggac gacgacgagc ggcccgtggg tcacggccgt
     1681 ctgctccggg tcgcggtcgt gcctcgggcg aggtcggccg ccttgcgctc ctgcgccgcg
     1741 gcgatggcca ccggctcgct gctcccctgc ggtgcgggag ccgtgaccat cgccatggcc
     1801 tccttggcag ccgggtcacg tgcgagcttg cgcaccaggg cgccggcctt ggccaccgac
     1861 ggggactccc cggcggcaac ggcctggctg gcggtggtgc ggctgtgcgt gacgacctcg
     1921 atcccctgga gcagggcggc ctggtagatc tcgtccgggt cgagccggcg cagctcgtcg
     1981 gcgaggcact cgggcatgtg gcgccgggcc aggctgatcc gccggtccgg ctgaccctgc
     2041 tgggacagcg tcgcgacggt gtcaccgggg cggaccaggt cgatggcgcc gctcgtgcgc
     2101 tccaggcgga cgctgctgat gccgctgccg cccggcgtgc gcacgatctt gacggggcac
     2161 tcgaggaagt gcgcgagcca gccggccaac aggtccgtcg acggagagtc cggggctccg
     2221 gccacgacgg ctcggatgat cggctcgaac ggtggctggt ccagggctgc agcgagcagt
     2281 cctcgccacc gggtgatccg cgtccaggcg aggtcggtgt caccggggtg gtagcagcgg
     2341 gctcgggccc tgagcgcgct tcgcgggctc gctgcggcgg ccgcgtcggt gatccggcgc
     2401 tgcgccatcc gcccgatcgg gtcgcccgac aggtccgccg gagccgtgtg gggccaccag
     2461 gcgaccacgg gggagtcggg gagcaggagc ggaacaacga cgctctggcc atgcttggcc
     2521 agctcgccga acaggcgcag gacgacgacc tcgctcgcgc ctgcgtcacc gccgaggcgg
     2581 atctgggcgt cgagccggct cgccccccgc ttgtcgccga gcacgacggt gacgatccgg
     2641 ctcgggtgct gccggcttgc ctcggcggcg atctcgatgg cctcgtcggc gtggcggtcg
     2701 tcgacgacga tgatgagcgt gagcacgcgc ccgagggcca tggctccgac gtcgttgcgc
     2761 acggtgacga gcttcttgct caccgcggcg ctcgtcgtcg cagggaggtt gatgatcatg
     2821 gcatcctcca gtggcggccg tcgcggcgca tcatgtcatc ggccgacggc ggtccccagc
     2881 caccggaggc gtagggctcc ggcgttccgc tgcgggccca cgacgtgatg agcgggtcga
     2941 ggatcgtcca ggagagctcg acctcctcgt gccgggggaa cagcggcggg tcgccgagca
     3001 ggacgtcgag gatgagccgc tcgtaggcct ccggcgaggt ctcggtgaag gcactgccgt
     3061 agccgaagtc catcgtgacg tcgcggacct ccatctgggc gccgggcacc ttggacccga
     3121 agcggatcgt cacgccctcg tccggctgga tccggatgac gatggcgttc tggccgagct
     3181 cctcggtcgc ggtgtcgttg aagggcaggt gcggtgcccg cttgaagacg accgccacct
     3241 cggtgacgcg cttaccgagc cgcttgcccg tgcgcaggta gaacgggacc ccggcccagc
     3301 ggcgcgtgtc gatgtcgagg cgtaccgcgg catacgtctc ggtggccgag gccgtgctga
     3361 cgccgtcctc cccgaggtac ccacggaccg gctccccgcc ctgccagccg gcggcgtact
     3421 gaccccgcgc cgaagccgtg cccaggtcct tgggcaggcg gacggccgag aggaccttct
     3481 ccttctcggc gcgcaggtcc cgcggcccga aggagacggg ctcctccatc gcgacgaggg
     3541 cgagcagctg gagcagatgg ttctggatga cgtcgcgagc cgcgccgata ccgtcgtagt
     3601 agcccgcccg gccggcgatg ccgatgtcct cggccatggt gatctggacg tggtcgacgt
     3661 agttggcgtt ccacacgggt tcgaacatct ggttggcaaa gcgcagcgcc atcatgttct
     3721 ggacggtctc cttgccgagg tagtggtcga tccggaagac cgcgtccggt gggaagacgc
     3781 cctcgacgat cgcgttgagc tcgcgggcgc tgccgaggtc gtgcccgaag ggtttctcga
     3841 tgatgacccg ccgccagctc ttggggtcgg gccgcgacag gccgcagcgc tcgagctgct
     3901 ggcagacctg ggcgaagaac cccggtggga tcgacaggta gaacgcctgg ttgcctccgg
     3961 tgccgcgctc ctcctcgagc cggtggacgg tcttggcgag caggtcgaag gccgagtcgt
     4021 cgtcgaaggc ccccggcacg aaacggaacc cctcggccag gttgcgccag acgtcctcac
     4081 ggaacggggt tcgcgcgtac tgccgcaccg cgtcgtggac gacctgcccg aagtcctggt
     4141 ccgcccagtc gcggcgcgcg aaccccgttc cgtga
//
LOCUS       DATAIX010000402         1677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_82035, whole genome shotgun sequence.
ACCESSION   DATAIX010000402 DATAIX010000000
VERSION     DATAIX010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82035"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1432)
                     /locus_tag="VHM65_06105"
     CDS             complement(<1..1432)
                     /locus_tag="VHM65_06105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX2705312.1"
                     /translation="MRRRPASRPSRRRTVITEAEFLARRESVERVMVVREKDERTQIG
                     VLEDGVLVEHYISRETTVSMAGNIYLGRVQNVLPSMEAAFVDIGKGRNAVLYAGEVNW
                     DAAGLENNAPKRIENALSSGENVLVQVTKDPIGHKGARLTSQISLPGRYLVYVPNSSM
                     TGISRKLPDIERTRLKRILRDIVPNTSGVIVRTAAEGASEEELRADVERLTATWEEIS
                     AKAGPKSKGRGKGSGSTPVLLHGEPDLTVRVIRDIFNEDFTSLVVAGDRSWDEVSSYV
                     ESVAPDLFPRVSRWTGPADLFTHYRVNEQLAKAMDRKVWLPSGGSLVIDRTEAMTVVD
                     VNTGKFVGSGGNLEETVTKNNIEAAEEIVRQLRLRDIGGIIVIDFIDMVLESNRELVV
                     RRLLECLGRDRTKHQVAEVTSLGLVQMTRKRVGSGLIEVFSEMCERCNGRGIIVHSEP
                     VDQPTGNGPANGHSGGHASAAPAGGQS"
     gene            1392..>1677
                     /locus_tag="VHM65_06110"
     CDS             1392..>1677
                     /locus_tag="VHM65_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705313.1"
                     /translation="MRRREGREAGRRRTVITEAEFLARRESVERVMVVREKDERTQIG
                     VLEDGVLVEHYISRETNVSMAGNIYLGRVQNVLPSMEAAFVDIGKGRNAVL"
BASE COUNT          249 a          564 c          587 g          277 t
ORIGIN      
        1 ggctctgccc gcccgccggc gcggcggagg cgtggccgcc gctgtggccg ttggcagggc
       61 cgttgcccgt cggctggtcg accggctcgg agtgcacgat gatgccccgg ccgttgcagc
      121 gctcgcacat ctcggagaac acctcgatga ggcccgaacc gacccgcttg cgcgtcatct
      181 ggacgagacc gagcgaggtc acctcggcca cctggtgctt ggtccggtcg cggccgaggc
      241 actcgagcag ccgccgcacg acgagctcac ggttcgactc gagcaccatg tcgatgaagt
      301 cgatgacgat gatcccgccg atgtcgcgca gccgcagctg acgaacgatc tcctcggcgg
      361 cctcgatgtt gttcttggtc accgtctctt cgaggttgcc ccccgagccg acgaacttgc
      421 cggtgttgac gtcgaccacg gtcatggcct cggtgcggtc gatgacgagc gatccgcccg
      481 agggcagcca caccttgcgg tccatggcct tggccagctg ctcgttgacg cggtagtggg
      541 tgaagaggtc ggccgggccg gtccagcgcg aaacccgggg gaacaggtcc ggggcgacgg
      601 actcgacgta cgagctgacc tcgtcccacg accggtcacc ggcgacgacg agcgaggtga
      661 agtcctcgtt gaagatgtcc cggatgacgc ggacggtcag gtccggctcg ccgtggagca
      721 gcaccggggt cgagccgctg cccttgccgc gtcccttcga cttcggcccg gccttggccg
      781 agatctcctc ccacgtggcg gtgaggcgct cgacgtcggc gcgcagctcc tcctcggagg
      841 caccctcggc cgcggtccgc acgatgacgc ccgaggtgtt cggcacgata tcgcgcagga
      901 tccgcttgag ccgggtccgc tcgatgtcgg ggagcttgcg cgagatgccg gtcatcgagc
      961 tgttgggaac gtagacgagg tagcgaccgg gcagcgagat ctgcgaggtg agccgggcac
     1021 ccttgtgacc gatcgggtcc ttggtcacct ggacgaggac gttctcaccc gaggacaggg
     1081 cgttctcgat gcgcttgggg gcgttgttct ccagcccggc ggcgtcccag ttgacctcgc
     1141 cggcgtagag gacggcgttg cgccccttgc cgatgtcgac aaaggcggcc tccatcgagg
     1201 gcaggacgtt ctggacccgc ccgaggtaga tgttgcccgc catcgacacc gtggtctcgc
     1261 gcgagatgta gtgctcgacg aggacgccgt cctcgaggac accgatctgg gtgcgctcgt
     1321 ccttctcccg caccaccatg acgcgctcga cgctctcgcg gcgggcgagg aactcggcct
     1381 cggtgatgac cgtgcgccgc cgcgagggcc gcgaggccgg ccgtcggcgc actgtcatca
     1441 ccgaggccga gttcctcgcc cgccgcgaga gcgttgagcg cgtcatggtg gtccgggaga
     1501 aggacgagcg cacccagatc ggagtcctcg aggacggcgt cctcgtcgag cactacatct
     1561 cgcgtgagac caacgtgtcg atggcgggca acatctacct cgggcgggtc cagaacgtcc
     1621 tgccctcgat ggaggccgcc tttgtcgaca tcggcaaggg gcgcaacgcc gtcctct
//
LOCUS       DATAIX010000403         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_51954, whole genome shotgun sequence.
ACCESSION   DATAIX010000403 DATAIX010000000
VERSION     DATAIX010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51954"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(477..1952)
                     /locus_tag="VHM65_06115"
     CDS             complement(477..1952)
                     /locus_tag="VHM65_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705314.1"
                     /translation="MNEHVTPAPRAASFDLAAMAVVIHRLTVTHPSVVSEARRWSTGQ
                     RGAALAAEEMAGADLGAFVTQALAVGSQAIALAAGAQDTFELERLVTEVGAKTAESSA
                     KAAEVTGTAVTAAAEAVSKAVAEANKAFVDADAQTRKSLAETVGAANASLQSEVQRIF
                     GGDNPELLAKLKPLLESVGHQIGEQALEQTDKLLAKVGRQFDPADPTSPFAKQAAALA
                     EQQKSLTASMDKNHLALVGKVDELARAVEVQKAAQAAVARTAKVTPLKGGAYETAVNA
                     VMDAIAAGLGDEYTESGTIVGALPGCKKGDGVLTIKGGLARVVVEMHDSGDRRVWNDY
                     LDEAERNREAAASIGLVPCADQNGGQTIRVLGARRVVVAFGPATDEPDLLRTVVQLMR
                     VSAIAASSRRDVEDLATAEEHIHAAEQLLERVNKIRSSAGTIRKSADAIEKECNTVQT
                     GMQNHLGQALDALAGVALEPVGLVELVGDASHKSGSAFDAA"
     gene            2149..>2454
                     /locus_tag="VHM65_06120"
     CDS             2149..>2454
                     /locus_tag="VHM65_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705315.1"
                     /translation="MSRPSALQLSLADIARLAHVSRPVASMWRRRELADHPFPEPVAV
                     VGGQERFDASEIADYLASTGRGSNPDARDDVAAHARLVTVTQVDEPATVDGLSALLCL
                     "
BASE COUNT          330 a          842 c          876 g          406 t
ORIGIN      
        1 gcgggacaac cctcatcggg tcggcaagcc tcttcgtctg cggctggagg gcttccactc
       61 cgcccggcgg ggccagtacc gggtgatcta ccggatcctc gacaccgagg tcgtcgtcga
      121 ggtggttaag atctcccacc gcagtgacgc ctacggatag tcgtgaacgg acccgcgctc
      181 gaaggagccg acgacgtgcc ttcaggtggg tgctccaggg tcgatgtctg gcctgtagcg
      241 agctgcgtca cggtttcgag gtcggtcgta gctcggtgcg gggtcgtcat atggcacagg
      301 actggtcagg tgcacctcgc gcatcgtgac ctaccagacc cgcgaacgcg gttggggtgg
      361 ggtccagcct tctgtgctga ccctctctac cttctgctgg caacgcggtc atgacgtttg
      421 cggttggctg gatgggcggg tcggccgccc ccctgctcgc ggccgacccg ggttggtcag
      481 gccgcgtcga aggcggaccc ggacttgtgg gacgcgtcgc cgacgagctc gacgagcccg
      541 acgggctcca gggcgacgcc ggccagcgcg tcgagcgcct gaccgaggtg gttctgcatc
      601 ccggtctgca ccgtgttgca ctccttctcg atggcgtcgg cgctcttgcg gatggtgccg
      661 gcgctggagc ggatcttgtt gacccgttcg aggagctgct ccgcggcgtg gatgtgctcc
      721 tcggctgtgg cgaggtcctc gacatcgcgc cgcgaggacg ccgcgatggc gctcacccgc
      781 atgagctgca ccacggtgcg aagcaggtcg ggctcgtccg tcgcggggcc gaaggccacc
      841 acgacacgac gggccccgag cacccggatc gtctggccgc cgttctggtc cgcgcagggc
      901 acgaggccga tggacgcggc ggcctcacgg ttgcgttcgg cctcgtcgag atagtcgttc
      961 cagacgcggc ggtcgcccga gtcgtgcatc tcgacgacga cacgcgcgag cccgcccttg
     1021 atggtgagca cgccgtcgcc cttcttgcag ccgggcaggg cgccgacgat ggtccccgac
     1081 tcggtgtact cgtcgccgag cccggcggcg atggcgtcca tgaccgcgtt gacagcggtc
     1141 tcgtaggcgc cgcccttgag cggtgtgacc ttggcggtgc gggcgaccgc tgcctgtgca
     1201 gccttctgga cctccacggc cctggcgagc tcgtcgacct tgccgacgag ggcgaggtgg
     1261 ttcttgtcca tcgacgcggt gagcgacttc tgctgctcgg ccagcgccgc ggcctgcttg
     1321 gcaaacggcg aggtcgggtc ggccgggtcg aactgccggc cgaccttcgc caggagcttg
     1381 tcggtctgct ccaaggcctg ctcaccgatc tggtgaccga ccgactccag tagcggcttg
     1441 agcttggcca gcagctcggg gttgtcaccg ccgaagatcc gctggacctc cgactgaagc
     1501 gacgcgttcg ccgccccgac cgtctcggcg aggctcttgc gggtctgcgc gtcggcgtcg
     1561 acgaacgcct tgttggcttc ggcgaccgcc ttgctcaccg cctcggcggc ggccgtgacg
     1621 gccgtcccgg tgacctcggc ggccttggcg ctcgactctg cggtcttggc ccccacctcg
     1681 gtgacgagcc gctccagctc gaaggtgtcc tgggcgccgg cggcgagggc gatggcctgc
     1741 gacccgacag cgagggcctg ggtgacgaag gcgccaaggt cggcgcccgc catctcctcg
     1801 gccgcgaggg ctgctccgcg ctggccggtg gaccagcggc gggcctcgga gacgaccgac
     1861 ggatgggtaa ccgtcaggcg gtggatgacg accgccatgg cggccaggtc gaaggacgcc
     1921 gcacgcggcg ccggtgtgac gtgctcgttc atgatgcgtg acctctcgtt cgccggccgc
     1981 gacgatcgcg gcccgtggac cgagactgac ccccccaggc gatcctgacg aatcagcaag
     2041 gtgataacca gggttatcac cctcaacgat agccgaatgc acgtgtaccg cctatggtga
     2101 tgtcggacgc accagacagg ccggtcgccg acggagagag gggcagccat gtcgagacca
     2161 tcggcactcc agctctccct cgccgacatc gcccgcctcg cccacgtgag ccgcccggtc
     2221 gcgtcgatgt ggcgccgccg ggagctggcg gaccacccgt tcccggagcc ggtcgccgtg
     2281 gtcggggggc aggagcggtt cgacgcgtcc gagatcgctg actacctggc atcgaccggc
     2341 cgcgggagca acccagatgc ccgagacgac gtggccgcgc atgcccggct ggtcaccgtg
     2401 acgcaggtcg acgagccggc gacggtcgac gggctcagcg ccctgctctg tctc
//
LOCUS       DATAIX010000404         1894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_111717, whole genome shotgun sequence.
ACCESSION   DATAIX010000404 DATAIX010000000
VERSION     DATAIX010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111717"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VHM65_06125"
     CDS             complement(<1..183)
                     /locus_tag="VHM65_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705316.1"
                     /translation="MTSPLAAQPLPPVCGIVPPYLLTALARNGDATAARRARRTIARD
                     ARVRGWRELRSSKPMPG"
     gene            521..>1894
                     /gene="leuA"
                     /locus_tag="VHM65_06130"
     CDS             521..>1894
                     /gene="leuA"
                     /locus_tag="VHM65_06130"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX2705317.1"
                     /translation="MIHPQQPSGMPTAKYVPFRDQIVVDLPDRTWPAKTIGAAPRWCA
                     VDLRDGNQALIDPMNAERKLRMFQLLVRMGYKEIEVGFPSASQTDYDFCRELIDGGHV
                     PDDVTIQVLTQSRDHLIERTFDAIRGSSSAIVHFYNSTSILQRRVVFGLDQDGIVDIA
                     VQAARLCRKLEETVPETTVYYEYSPESYTGTELDFAMRICNAVADVIDPTPDHKMIIN
                     LPATVEMITPNVYADSIEWMVRQLERRDSVVVSLHPHNDRGTGVAAAELGYLAGADRI
                     EGCLFGNGERTGNVCLVTLGMNLFSQGVDPQIDFSDIDEIRRTVEYCNQLPVHPRHPY
                     GGDLVFTAFSGSHQDAIKKGLEDMDRQVAASGKGVNELIWGVPYLPIDPHDLGRSYEA
                     VVRVNSQSGKGGVAYILKTEHGLDLPRRLQIEFSGVVQRDVDQDGGEVSPAELWAKFE
                     NEYLPTPH"
BASE COUNT          316 a          697 c          617 g          264 t
ORIGIN      
        1 gccgggcatc ggcttggagc tgcgcagctc gcgccacccg cgcacccgcg cgtcgcgggc
       61 gatcgtgcgc cgggcccggc gggctgcggt cgcgtcgcca ttgcgagcca acgcggtgag
      121 caggtagggc gggacgatcc cgcagacggg cgggagcggc tgggcggcga gtggcgacgt
      181 cataccccaa actatccctg ccatcaccgg catcctcacc caggaggagg gtcctcgtcc
      241 cggaggcgct caacgccgac agcgccctgt tcaccctcct gacacgcccc gggaaaaacc
      301 cccgggggtt tcacgatctg gacgcgacgg cgtggcgagt tggacgcgga cgccgcgggc
      361 gccctaccgt gtggtcatgc gtcacgcgtc gggagcgctg ctcctccttc gtcgccgcgg
      421 cggggcccac taacacggcc gccctcgtcg cggagtgtcg tgcgtccggc cgggccccaa
      481 cgaacaccca ccgacagcga cgagagcgtg aggcgccacc atgatccacc cccagcagcc
      541 gtccggcatg ccgacggcaa agtacgtccc cttccgagac cagatcgtcg tcgacctgcc
      601 cgaccgcacc tggccggcca agacgatcgg ggcggccccg cgctggtgcg cggtcgacct
      661 gcgcgacggc aaccaggcac tcatcgaccc gatgaacgcc gagcgcaagc tgaggatgtt
      721 ccagctcctc gtgcggatgg gctacaagga gatcgaggtc ggcttcccgt cggccagcca
      781 gaccgactac gacttctgcc gcgagctcat cgacggtggc cacgtgcccg acgacgtgac
      841 gatccaggtc ctcacccaga gccgcgacca cctcatcgag cggaccttcg acgcgatccg
      901 gggctcgagc tcggccatcg tccacttcta caactcgacc tcgatcctgc agcggcgggt
      961 cgtcttcggc ctggaccagg acggcatcgt cgacatcgcc gtccaggcgg cccggctctg
     1021 ccgcaagctc gaggagacgg tgcccgagac caccgtctac tacgagtact cgccggagtc
     1081 ctacacgggc accgagctgg acttcgccat gcggatctgc aacgcggtcg ccgacgtcat
     1141 cgaccccacg ccggaccaca agatgatcat caacctgccc gcgacggtcg agatgatcac
     1201 gcccaacgtc tacgccgact cgatcgagtg gatggtgcgc cagctcgagc ggcgcgactc
     1261 ggtcgtggtg tcgctgcacc cgcacaacga ccgcgggacc ggcgtggccg cggccgagct
     1321 cggctacctc gccggcgccg accgcatcga ggggtgcctg ttcggcaacg gcgagcgcac
     1381 cggcaacgtc tgcctcgtca ccctcggcat gaacctgttc agccagggcg tcgacccgca
     1441 gatcgacttc tccgacatcg acgagatccg ccggacggtc gagtactgca accagctgcc
     1501 cgtccacccc cggcatccgt atggcggcga cctcgtcttc accgcgttct cgggctcaca
     1561 ccaggacgcg atcaagaagg gcttggagga catggaccgc caggtcgcgg cctcgggcaa
     1621 gggggtcaac gagctcattt ggggcgtgcc ttacctgccg atcgacccgc acgacctggg
     1681 ccggtcctac gaggccgtgg tccgggtcaa cagccagtcc ggcaagggcg gcgtcgccta
     1741 catcctcaag accgagcacg gcctggacct gccgcgacgg ttgcagatcg agttctccgg
     1801 ggtcgtccag cgcgacgtcg accaggacgg cggcgaggtg tcgccggccg agctctgggc
     1861 caagttcgag aacgagtacc tgcccacccc gcac
//
LOCUS       DATAIX010000405         1640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_21896, whole genome shotgun sequence.
ACCESSION   DATAIX010000405 DATAIX010000000
VERSION     DATAIX010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21896"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1617)
                     /locus_tag="VHM65_06135"
     CDS             complement(<1..1617)
                     /locus_tag="VHM65_06135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2705318.1"
                     /translation="MRGPADDPAWARPSLLVLLVATAALYLVNLTANGWANAYYSAAA
                     QAGSSSWSAFFFGSSDAGNSITVDKTPASLWAMALSVRLLGLSSFAILLPEALMGVAS
                     VGVLYATVKRHFGAGAGLIAGAVLALTPVAVLMFRFNNPEALLVLLMTLAAWATVKSV
                     ERASPKWFMLVGVFIGLGFLTKMLQVLLVVPAFALAYLVAAPTTLRRRIWHSVLGALA
                     IVVSTGWWIAVVELLPTNLRPYIGGSQTNSVLELTLGYNGLGRLNGNEAGSVVPGGNP
                     GAGMWGETGWLRMFGSSIGGQVAWLLPAALVLLVAGLALRGRRPRTDARRAAYLAWGG
                     WLVVTTVTFSLMAGIFHDYYTIALAPAIGALVGMGLAELWERRESAAIRVVLAVLLAG
                     TSVWAFVLLARTETWLPWLRMVALGLGMACAVLLLVVTAMPRRAVPALVGAALVAALA
                     APTAYSLQTAGTAHSGSIVSAGPSTGRGGMGGTGGRGGFPGGLPGTQGGLGATRQGPG
                     GTPLAPFGQAGPGQAGPGRAGGAQGGTGAGG"
BASE COUNT          266 a          575 c          628 g          171 t
ORIGIN      
        1 gccgcctgcg ccggtgccgc cctgtgcgcc cccggctcgc ccgggaccgg cttgcccggg
       61 accggcttgc ccgaaggggg cgagcggcgt cccgccggga ccctggcgcg tcgcacccag
      121 gcctccctgg gtgccgggca gcccgccggg gaagccgccg cgaccaccag tgccgcccat
      181 gccgccacga ccggtcgacg gcccggcgct gacgatggag cccgaatgtg cggtgcccgc
      241 tgtctgcagg ctgtacgccg tcggcgcggc cagtgccgcg accagcgcgg caccgacgag
      301 ggccgggacg gcccggcgcg gcatggccgt gacgacgagc agcagcacgg cgcacgccat
      361 gccgagtccg agggccacca tgcggagcca gggcagccac gtctcggtgc gtgcgagaag
      421 gacgaacgcc cacacactcg tcccggcgag cagcacggcg agcacgaccc ggatggcagc
      481 ggactcgcgg cgctcccaga gctcggcgag gcccatcccc accagcgccc cgatcgccgg
      541 tgccagcgcg atcgtgtagt agtcgtggaa gatgccggcc atgagcgaga acgtcaccgt
      601 cgtgacgacg agccaacccc cccaggcgag gtatgccgcg cgcctcgcgt ccgtgcgcgg
      661 ccgacgcccg cgcagtgcca ggccggcgac gagcaggacg agcgcggccg ggagcagcca
      721 ggcgacctgc ccgccgatgg agctcccgaa catccgcagc cacccggtct cgccccacat
      781 cccggcgccg gggttgccgc cgggcacgac cgagccggcc tcgttgccgt tgaggcggcc
      841 caggccgttg tagccgagag tgagctcgag gaccgagttg gtctgcgagc cgccgatgta
      901 ggggcgcagg ttcgtcggga gcagctcgac gacggcgatc caccagccgg tgctgacgac
      961 gatggccagg gcgccgagga cgctgtgcca gatccgcctg cgcagggtcg tcggagcggc
     1021 gacgaggtag gcgagggcga atgccgggac gacgagcagg acctggagca tcttggtgag
     1081 gaaccccagg ccgatgaaga cgccgacgag catgaaccat ttggggctgg cccgctcgac
     1141 cgacttcacg gtcgcccagg cggccagggt catgagcagg acgagcagcg cctcggggtt
     1201 gttgaagcgg aacatgagca cggcgaccgg cgtcagggcg agcacggctc cggcgatgag
     1261 cccggcgccg gcgccgaagt ggcgcttcac cgtcgcgtag aggacgccga cgctcgcgac
     1321 gcccatgagc gcctccggca gcaggatggc gaacgagctg agcccgagca gccggacgga
     1381 cagggccatc gcccagagcg aggccggcgt cttgtcgacg gtgatcgagt tgccggcatc
     1441 ggaggagccg aagaagaacg cggaccagct cgagctgccg gcctgggccg ctgcggagta
     1501 gtaggcgttg gcccagccgt tcgcggtgag gttgacgagg tagagcgcgg ccgtggcgac
     1561 cagcaggaca agcagcgagg ggcgagccca ggcggggtca tctgcaggcc cgcgcaccag
     1621 acggcccagc cgggtgggcg
//
LOCUS       DATAIX010000406         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_52209, whole genome shotgun sequence.
ACCESSION   DATAIX010000406 DATAIX010000000
VERSION     DATAIX010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52209"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..475
                     /locus_tag="VHM65_06140"
     CDS             257..475
                     /locus_tag="VHM65_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705319.1"
                     /translation="MDAVDAVDATATEGAKPVPSEQDPVVAVVGTSQTAGEAGSLGAA
                     IANTLRLARWRRRVSQRDLAADLGVAQS"
     gene            512..751
                     /locus_tag="VHM65_06145"
     CDS             512..751
                     /locus_tag="VHM65_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705320.1"
                     /translation="MGEIVRVVESLGFVLVLVDLDPDDDGPGSGQVIARDAAGRTFPA
                     HAELVPQGLPPHYWFVRHPGAPRREWPPWRWATQR"
     gene            complement(807..>2030)
                     /locus_tag="VHM65_06150"
     CDS             complement(807..>2030)
                     /locus_tag="VHM65_06150"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase"
                     /protein_id="HEX2705321.1"
                     /translation="DGIVVTPSHNPPADGGFKYNPTHGGPADTDATSWIARRANEIIA
                     AGMVDVRRVSFEKARAAAGSYDFLGTYVDDLPGVVDLARIRDAGVRIGADPLGGASVA
                     YWGEIGERHGLDLTVVNPDTDPAWPFMTLDWDGKIRMDCSSPYAMASLIERRASYDIA
                     TGNDADADRHGIVTPDAGLMNPNHYLAVAISYLYGGARPGWGADTAIGKTLVSSSMID
                     RVAASLGRRLIEVPVGFKWFVPGLLDGSVGFGGEESAGASFLRFDGSVWSTDKDGIIL
                     ALLASEVLAATGRTPSQHYADLVARHGDPAYARIDAPASREEKARLSALSPEAVTARM
                     LAGEPIIDRLTAAPGNGAKIGGLKVTTEHAWFAARPSGTEDVYKIYAESFLGPAHLAA
                     VQSEARDVVSAALAG"
BASE COUNT          302 a          684 c          762 g          282 t
ORIGIN      
        1 ccggagacta tgcggtgagt cgccaccgca cttaacgccc ggcttctggc ggaggtccgt
       61 tgaggcgcat tgagcctgcg ggtgagtcac cgcagagcat gccgcgacgg caggagcccc
      121 ggccgcggcc agagatcatc cggcaccaca accggtctct ccacatcggg tcgcccggtt
      181 gacccaccct caacaggtct cggggggtcg ctggcggatc ggtgcgggac cgggtgagga
      241 tgcgagggtg gacgcggtgg acgccgtgga cgccgtggac gccacagcga cggaaggggc
      301 caagccggtg ccaagtgagc aggacccagt cgtggctgtc gtcggcacgt cgcagaccgc
      361 gggtgaggcg gggagcctcg gtgcggcgat cgcgaacacc ctacgtctcg cgcgctggcg
      421 aaggcgggtg agccagcgcg atctggcggc agacctcggc gtggcccaga gctgagtggc
      481 gcgcctcgag cgaggggtcc ttccgcaggc catgggcgag atcgtgcgcg tcgtggagtc
      541 acttgggttc gtgctcgtcc tcgtcgacct ggaccctgac gatgacggac ccggatctgg
      601 gcaggtcatc gcccgtgacg cggcggggcg gaccttcccc gcgcacgcgg agctggtgcc
      661 gcaggggctg ccgccgcact actggttcgt gcgacacccg ggtgcaccgc ggcgagagtg
      721 gccgccctgg cgatgggcga cgcagcggta acgacacggc gcccgacgca acggcgcccg
      781 acgacacggc gcccagccat ggagcgctac ccggccaggg cggccgagac gacgtcgcgg
      841 gcttcggact ggaccgccgc gaggtgggcg gggcccagga aggactccgc gtagatcttg
      901 tagacgtcct cggtccccga cgggcgagcc gcgaaccacg cgtgctcggt cgtcaccttg
      961 agcccgccga tcttggcgcc gtttccgggc gctgcagtga gccggtcgat gatcggctca
     1021 ccggccagca tcctcgccgt gaccgcttcg ggggagagcg ccgacagccg ggccttctcc
     1081 tcgcggctgg ccggtgcgtc gatccgggcg taggccggat caccgtggcg ggcaacgaga
     1141 tcggcgtagt gctggctcgg ggtccggccg gtggccgcga gcacctccga ggcgagcaga
     1201 gccaggatga tgccgtcctt gtcggtcgac catacggacc cgtcgaagcg caggaatgac
     1261 gctcccgccg actcctcacc gccgaacccg acggacccgt cgagcagccc gggaacgaac
     1321 cacttgaaac cgacggggac ctcgatcagg cgccgcccga gcgaagcggc cacccggtcg
     1381 atcatcgagg aggacacgag cgtcttgccg atcgccgtgt cggcacccca cccgggccgg
     1441 gcgccgccat agaggtagga gatcgcgacg gcaaggtaat ggttggggtt catgaggccc
     1501 gcgtccgggg tgacgatccc gtggcggtcg gcgtcggcgt cgttgcccgt ggcgatgtcg
     1561 tatgacgcgc ggcgctcgat gagcgaggcc atggcatagg gcgaggagca gtccatgcgg
     1621 atcttgccgt cccagtcgag cgtcatgaac ggccaggccg ggtcggtgtc ggggttgacg
     1681 acggtcaggt cgagaccgtg ccgctctccg atctcgcccc agtaggcgac cgacgcgccg
     1741 ccgagcgggt ctgcgccgat ccgcaccccc gcgtcccgga tcctggccag gtcgacgacg
     1801 ccagggaggt cgtcgacgta cgtgccgagg aagtcgtagg acccggccgc ggcccgcgcc
     1861 ttctcgaagg acacccggcg gacgtccacc atccccgcgg cgatgatctc gttggcgcgc
     1921 cgcgcgatcc acgacgtcgc gtcggtgtcg gccggaccgc cgtgcgtcgg gttgtacttg
     1981 aacccgccgt cggcgggagg gttgtgcgac ggggtgacga cgatcccgtc
//
LOCUS       DATAIX010000407         1876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_82407, whole genome shotgun sequence.
ACCESSION   DATAIX010000407 DATAIX010000000
VERSION     DATAIX010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82407"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VHM65_06155"
     CDS             <1..454
                     /locus_tag="VHM65_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705322.1"
                     /translation="RDRLAGAQVVNPVKITASSAAPQHPATLARDGATNTFWSPAGRG
                     SGASEFIEATFAGSFRLVAIQVFNGSSEQPKPYLTTWRLATVLLTTTKADGGVETRAL
                     TLQDVPGRQDFSLGVSDVTRVRITVQTAFGATPARQIALAEVAFFKRS"
     gene            complement(441..>1876)
                     /locus_tag="VHM65_06160"
     CDS             complement(441..>1876)
                     /locus_tag="VHM65_06160"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX2705323.1"
                     /translation="LHDVARHRGLVWLRRLAYVVALCRTAQPRRCAEARALAVERRRV
                     GDEWGAALIESSVAFALLRRGRPDIELLEELTGRFRLLGSPQLEAWARSAHALAAAEA
                     DLPDFGPPESAGAFARAAGAPGALALAYAALAASRPDQGAELLRLAESTARESGLGCR
                     PWTWKRDAASPSSPATAVWGPLQQATVTGHLDGAATTSASARHTPLVWSGLTLTCFGE
                     FSVTAHGRSVDLAGVRPVALTVLRILAAHAGTPVHREVIVDALWGELRETAALHNLHV
                     AVSSLRQGLESVVPGRSRQLLARQGQAYVLAPGTERITDLQRLDAHLAAATRCRHAGD
                     PTGQIQALREAIDVYVGVVLPGDGAAEWVLAIRDRYRQAAAGAAARLAELELGRECAA
                     AAAAAAHRSLQIDPWRDASWHALILAHDRAGEPAEAERARQEYAAMLDSLGVRTMARP
                     PARPAAVTPSATATAARPAQRPPVRTA"
BASE COUNT          269 a          656 c          712 g          239 t
ORIGIN      
        1 gcgcgaccgg ctcgctggtg cgcaggttgt caacccggtc aagatcaccg cgtccagtgc
       61 cgcaccccag cacccggcga ccctggccag ggacggcgcc acgaacacgt tctggtcgcc
      121 ggccggccgg gggtccggtg cgtccgagtt catcgaggcc accttcgcag ggtccttccg
      181 gctggtcgcg atccaggtct tcaacgggtc ctccgagcag cccaagccct acctgacgac
      241 atggcgtctg gcaacggtcc tgctcaccac gacgaaggcc gacggcgggg tcgagacccg
      301 ggcgttgaca ctgcaggacg tgccagggcg gcaggacttc tcgctcgggg tcagcgacgt
      361 cacgcgggtg cgcatcacgg tgcagacggc cttcggcgcc acccctgcga ggcagatcgc
      421 actggcagag gtggccttct tcaagcggtc ctgacgggtg ggcgctgggc cggcctagcg
      481 gcagtcgccg tggctgaggg ggtcacggcg gccggacggg cagggggtcg cgccatcgtg
      541 cgcaccccga gcgagtcgag catggcggca tactcctgcc tggcgcgctc ggcctccgcg
      601 ggttccccgg cccgatcgtg cgccaggatg agcgcgtgcc aggaggcgtc ccgccaaggg
      661 tcgatctgga ggctgcggtg agccgccgcg gccgcggccg cggcgcattc ccggcccagt
      721 tcgagctcgg ccagccgtgc ggcagcgccg gcggcggcct ggcggtaccg gtcgcggatg
      781 gccaggaccc actccgcggc gccgtctccg gggagcacga cgccgacata cacgtcgatg
      841 gcctcacgca gggcctggat ctgtcccgtc ggatcgccag cgtgccgaca ccgggtggcc
      901 gcggcgagat gggcgtcgag ccgctggagg tcggtgatgc gctcggtgcc cggagcgagg
      961 acgtatgcct gaccttggcg ggcaagcagc tgacgcgaac gtccgggaac gacggactcg
     1021 agcccctggc gtaggctcga cacggccacg tggaggttgt gcagcgcagc ggtctcgcgc
     1081 agctcacccc atagggcgtc gacgatgacc tcgcgatgca cgggggtgcc cgcatgggcc
     1141 gcgaggatgc gcagcacggt gagggcgacc ggtcggacgc cggccaggtc tacggaccgg
     1201 ccgtgggccg tcacgctgaa ctcgccgaag cacgtcaggg tcaggccgct ccagaccagc
     1261 ggcgtatgcc gtgcactcgc cgaggtcgtg gcggccccat ccaggtgacc cgtgacggtg
     1321 gcttgctgga gggggcccca gactgcggtg gcggggctgg acggcgacgc tgcgtcgcgc
     1381 ttccaggtcc agggacggca gccgagaccg ctttcccttg ccgtggactc ggcgagcctg
     1441 agcagctcgg cgccctggtc gggccgggaa gcggccaggg cggcataggc caaggccagg
     1501 gccccaggtg caccagctgc gcgagcgaag gccccggccg actcgggtgg cccgaagtcg
     1561 gggaggtcgg cctccgcggc ggcgagggcg tgtgccgacc gcgcccaggc ctccagctgc
     1621 ggggagccga ggagtcgaaa acgtccggtc agctcctcca acagctcgat gtcgggccga
     1681 ccgcgacgca ggagggcgaa cgcgaccgag gactcgatga gcgccgcccc ccactcgtcc
     1741 ccgacccgcc gccgttcgac tgcgagtgcc ctggcctcag cgcaacggcg cggctgggcg
     1801 gtccggcaaa gggcgaccac gtacgcgagc cgacgaagcc agaccagccc tcgatggcgg
     1861 gccacgtcgt gtagtc
//
LOCUS       DATAIX010000408         3556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_112045, whole genome shotgun sequence.
ACCESSION   DATAIX010000408 DATAIX010000000
VERSION     DATAIX010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112045"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VHM65_06165"
     CDS             complement(<1..207)
                     /locus_tag="VHM65_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705324.1"
                     /translation="MFAGTPAAALASLDAVAASSHEVVAVVTRPDAPAGRGRHLGQSP
                     VRARAEELGLPVLTPPSLRDPVFQD"
     gene            complement(218..763)
                     /gene="def"
                     /locus_tag="VHM65_06170"
     CDS             complement(218..763)
                     /gene="def"
                     /locus_tag="VHM65_06170"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX2705325.1"
                     /translation="MSIKEIRLFGDPVLRTPASPVVDFDRELRRLVKDLADTMLDAPG
                     AGLAAPQIGVALRVFTYYIDGELGHLVNPDLELSIEGQDGPEGCLSFPGLVFPTPRAL
                     RVVATGFDEYGEPVTVEGTELLARAVQHETDHLDGILFIDRLDPTQRKLAMAAIREAD
                     WVGGRLPQVKVSPHATFGRAL"
     gene            complement(851..2425)
                     /locus_tag="VHM65_06175"
     CDS             complement(851..2425)
                     /locus_tag="VHM65_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX2705326.1"
                     /translation="MSTLAEGTDTHPGGLGEWDWMDPFELLVEVPGTIDPRVREALVT
                     VAGAGGPGALVGRDAAQALSVVEAVEAVKAWAEHVGLEAWARLREATHEDAEADPAVV
                     HGMWGDGSGGLSHEARRELDSCVVDEVALATGLGPVECHSRFRFATAEPARTATLRAR
                     MAAGRTGWWQARRVMEACQGMPDAVADQVAARVLAPARDGSLASSGTVSRRLARQVTL
                     HDPHAGERRRGEAFLRRDARVTLEDDGTGTLVITTTAARAVQAVERLDAAARAQRCAG
                     DPRTLGQLRSDIATSLLSAGTLTGSTGAIDESPDGAEGAPDSSQDNGPDGTGQREVSG
                     PAWYLPPPARVNVVVALSTLLGLDEQAGEIPGHGFLTAAHTRQVALAAGSIWRRLVVD
                     DLSGHAIEVSTHRYRPSEEMRTQVAARDGTCRGPGCTIPAAGCDLDHQRPWPQGPTSI
                     DNLNAKHRAHHNRKTRSLWRCDQDLPSDEVPEGSLPLDARERPGGAIRWTTRARRRYT
                     TYPAQYLHPDYDPLRE"
     gene            complement(2584..>3556)
                     /locus_tag="VHM65_06180"
     CDS             complement(2584..>3556)
                     /locus_tag="VHM65_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705327.1"
                     /translation="RDGPGARAHLPSLAWRTARTALERGPVLIQVPRRGYLPALSCQS
                     CRAPVHCLRCKGPVAVAAQGAAAACRWCGTLMGADGFVCPHCEGRALRSTVVGAGRTA
                     EELGRAFAGVPVHTSGGEAVLGRVSAAPGLVIATPGAEPVAEGGYAAVLLLDAWALLD
                     RPSLDAGSEALRRWMAAAALARTRADGGVVVLAGAPGQVTLAPVEALVRWDPGWLAER
                     ELAERRELRLPPAVTMAQVVGSRHTLAEVLACAELPGGAERFGPLPRSAGPGRHDQQG
                     LLQVLLRVEPAERAALAAALAHVRAVRSARKETDPLTIRLDPADPVA"
BASE COUNT          462 a         1300 c         1333 g          461 t
ORIGIN      
        1 gtcctgaaag acggggtcgc gcaggctggg tggggtgagc acgggcaacc ccagctcctc
       61 ggcccgggcc cggacgggcg actgcccgag gtgccgtccg cggccggccg gtgcgtccgg
      121 ccgggtcacg acggcgacga cctcgtggga cgaggcagcc acggcgtcga ggctggccag
      181 ggctgccgcg ggcgtgccgg cgaacacgac ccgcatctca gagcgcccgc ccgaaggtcg
      241 cgtgcgggct gaccttgacc tggggcaggc gcccaccgac ccagtccgcc tcgcggatcg
      301 cggccatggc gagcttgcgc tgggtggggt cgaggcggtc gatgaacagg atcccgtcga
      361 gatggtcggt ctcgtgctgg accgcccggg caagcagctc ggtgccctcg acggtgaccg
      421 gctcgccata ctcgtcgaac ccggtggcga ccacgcgcag cgcccggggc gtggggaaca
      481 ccagcccggg aaacgacagg cagccttccg gtccgtcctg cccctcgatg gacaattcga
      541 ggtccgggtt gaccaggtga ccgagctcac cgtcgatgta gtacgtgaag acccgcagcg
      601 cgacgccgat ctgcggggca gccaggccgg ccccaggggc atccagcatc gtgtcggcga
      661 ggtccttgac gagccggcgc agctcccggt cgaagtcgac gaccgggctc gccggggtgc
      721 gcagcacagg gtcaccgaag aggcggatct ccttgatcga cacgcgttgg agtgtatgcc
      781 gcgccgcgcg ggcccgcgtt cagaagggcg gcgggtcgtc ctccggcagt gctggtgggt
      841 cgttcgtggg ctattcgcgc agagggtcgt agtcggggtg caggtactgg gcggggtagg
      901 tcgtgtacct gcgccgggct ctcgtggtcc acctgatggc gccgccgggt cgttcgcggg
      961 cgtcgagggg cagggaaccc tcgggaacct cgtcgctggg caagtcctga tcgcagcgcc
     1021 agaggctgcg ggtcttgcgg ttgtgatggg cgcggtgctt ggcgttgagg ttgtcgatgc
     1081 tcgtgggtcc ctggggccag gggcgctggt ggtccaggtc gcagccggcg gcggggatgg
     1141 tgcagccggg tcctcggcag gtgccgtcgc gagcggcgac ctgggtgcgc atctcctcgc
     1201 tgggccggta acggtgggtg gagacctcga tggcgtgtcc gctcaggtcg tccaccacca
     1261 ggcgccgcca gatcgacccg gcggccaggg cgacctggcg ggtgtgggcg gcggtgagga
     1321 agccgtgccc gggaatctcc cctgcttgct cgtccagacc gagcagcgtc gagagtgcaa
     1381 ccacgacatt cacccgcgcc ggtggcggca ggtaccacgc cggcccgctc acctcacgct
     1441 ggccggtgcc gtccggcccg ttgtcctggc tgctgtctgg ggccccctcg gcgccgtcgg
     1501 ggctctcgtc gatggctccc gtgctgccgg tcagtgtgcc ggccgagagc aggctggtgg
     1561 cgatgtcgga gcggagctgg cccagggtgc gggggtcccc ggcgcagcgc tgggcgcggg
     1621 cggcggcgtc gaggcgctcg acggcctgga ccgcgcgggc cgcggtcgtc gtgatgacca
     1681 gcgtcccggt gccgtcgtcc tcgagggtga cgcgggcgtc acgacgcagg aacgcctcgc
     1741 cgcggcggcg ctccccggcg tgcgggtcgt gcagcgtgac ctgacgcgcc aggcgccggc
     1801 tcacggtgcc cgacgatgcg agcgacccgt cgcgggcggg agccaggacc cgggccgcca
     1861 cctggtcagc gactgcgtcc ggcatgccct ggcaggcctc catgacccgc cgggcctgcc
     1921 accacccggt gcgcccggcg gccatccgcg ccctcagcgt cgcggtccgc gccggctcgg
     1981 ccgtggcgaa gcggaaccgg gagtgacact ccaccggccc cagacccgtg gccagggcga
     2041 cctcgtccac gacacacgag tcgagctcgc gccgggcctc atgactgagc ccaccggagc
     2101 cgtcacccca catcccgtgc accaccgccg ggtcggcctc ggcgtcctca tgggtcgcct
     2161 cgcgcagccg cgcccacgcc tccaggccga cgtgctcggc ccaggccttg accgcttcga
     2221 cggcctcgac cacggacagt gcctgggccg cgtcacgccc gaccagcgcc cccggcccgc
     2281 cggccccggc cacggtcacc agcgcctcgc gcacccgcgg gtcgatggtg ccggggacct
     2341 cgaccaagag ctcgaacggg tccatccagt cccactcacc cagcccgcca gggtgggtgt
     2401 cggtgccttc ggctagtgtg gacatgtgtt cgattctagt tcgatggcgg aggtgtagtc
     2461 aagccgaaac tccatatata tcaacagtct tgacgccacc cgtcgcccgg cggggttgcc
     2521 tccggtgcgc ccctgaaagc acgcaggttg cgcaccgacg ggagagcagg atggccggcc
     2581 tggtcaggcc accgggtcgg ccgggtcgag acggatggtg agcgggtcgg tctccttgcg
     2641 ggcgctgcgg acggcccgga catgtgcgag agccgccgcc agcgccgccc gctcagcggg
     2701 ctcgacccgc aggagcacct ggagcagccc ctgctggtcg tggcgccccg ggccggcgct
     2761 cctgggcagc gggccgaagc gttctgcccc gcccggcagc tccgcgcagg cgagcacctc
     2821 ggcgagggtg tgacgcgacc cgaccacctg ggccatcgtc accgcaggcg gaagccgcag
     2881 ctcgcggcgc tcggccagct cacgctcggc cagccagccc gggtcccatc ggaccagggc
     2941 ctcgaccggc gccagggtga cctgacccgg cgctccggcg agcacgacca ctcccccgtc
     3001 ggcccgggtc ctggccaggg ctgccgccgc catccagcgc ctcagcgcct cagatccggc
     3061 atcgagcgag ggccggtcga gcagcgccca tgcgtcgagg agcagcaccg ccgcatatcc
     3121 gccctcggcc accggctcgg ccccgggagt ggcgatgacg agcccgggag ccgcgctcac
     3181 ccggccgagg accgcctcac caccggaggt gtgcaccggg acacccgcaa aggcccggcc
     3241 gagctcctcg gcggtccggc ccgccccgac gacggtcgag cgcagcgcgc gcccttcgca
     3301 gtgcgggcac acgaaaccgt cggctcccat gagggtccca caccatcggc aggccgctgc
     3361 ggcgccctgg gccgccaccg cgacggggcc cttgcagcgc aggcagtgca cgggcgcccg
     3421 gcagctctgg cacgacaggg ccggcaggta gccgcgccgg ggtacctgga tgagcaccgg
     3481 accgcgctcc agggcggttc gtgccgtacg ccacgccagg gagggcaggt gggcacgcgc
     3541 ccccggcccg tcacgc
//
LOCUS       DATAIX010000409         2993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_52487, whole genome shotgun sequence.
ACCESSION   DATAIX010000409 DATAIX010000000
VERSION     DATAIX010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52487"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VHM65_06185"
     CDS             <1..676
                     /locus_tag="VHM65_06185"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX2705328.1"
                     /translation="AGTATIVLTNEPERWVGRASYKLAGALDAWTPEGLAVAGRRCLD
                     IGASTGGFTHVLLERGAAAVLALDVGQGQLASTLRDDPRVTELSGTNIRDVLPGSLGP
                     SFGLVVADLSFISLEHVLPRIAEQLSPDGDAVVLVKPQFEVGRDRLGKGGVVRSAGDR
                     ARALRAVIGYAEAAQLGVRAIMASPIDGSAGNREYLLWVRPSLSGTLSRQELNDRVDE
                     AVRSRP"
     gene            724..1656
                     /locus_tag="VHM65_06190"
     CDS             724..1656
                     /locus_tag="VHM65_06190"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002892.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEX2705329.1"
                     /translation="MTRRIMLVSHPRRPDVAAAAREVTARFQQAGIEVLPAPDEVWAP
                     DRSGIEHLLPGAGATGARDCELVCVLGGDGTILRGAELTRGSETPLLGVNLGHVGFLA
                     EVEREDLAATVEHIARRRYTVEERMTLEVVASVNDQTIARSWALNEVSVEKAARERMI
                     EVAVEIDGRPLSTWGCDGVVMATPTGSTAYAFSAGGPVVWPDVEAILLVPNAAHALFA
                     RPLVVGPNSRLAIELIDERLDGDPGVCAVMWCDGRRYNDLPGGARVEVTRSDTPVHLA
                     HLDTGPFTDRIVAKFALSVQGWRGAARGQAPRGT"
     gene            1689..>2993
                     /locus_tag="VHM65_06195"
     CDS             1689..>2993
                     /locus_tag="VHM65_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2705330.1"
                     /translation="MFQEIRLRGLGVIDDAVLELGPGLNVLTGETGAGKTMVVSGLGL
                     LLGGRADSGLVRTGDSMATVEGTIELPAGHPALVRAQDAGGDTDDGLVLLRSVSSEGR
                     SRAHVGGRSAPVGTLAQIGRLLVAVHGQADQWRLRSSAQHREILDDFGGPPLREALTA
                     YLRCFDDWETTRAEALRLRALATASRRDAELLRAGLEYIEAVHPQPGEDLTLRVEDER
                     LAHADGLRTSALQAQALLAGGDDAAESPAGYGAPGGSVRELLSAARGAVAGMSVHDPA
                     LRELDGRLAELGYLVADLSADLAHYGAQVEVDPARLAWVQQRRSELASLVRRYGDSVD
                     DVLDWARESAARFEELAGAGERAELLEIEATALRAELARRAATLSQARSAAAAELSTR
                     ITGELSQLAMTSAAVHVAVRQRPDPEGLSVGGEPVRATRTGID"
BASE COUNT          382 a         1088 c         1110 g          413 t
ORIGIN      
        1 cgccggcacc gcgacgatcg tcctcacgaa cgaacccgag cgatgggtcg ggcgcgcgtc
       61 atacaagctc gccggtgcac tggacgcctg gacccctgag ggcctggccg tcgccgggcg
      121 gcgctgcctc gacatcgggg cgagcaccgg tggcttcacg cacgtgctcc tcgagcgtgg
      181 cgcagcggca gtgctcgccc tcgatgtcgg gcagggacag ctcgccagta cattgcggga
      241 tgacccccgg gtcaccgagc tgtccgggac caacatccgg gacgtccttc caggatcgct
      301 cggcccgtcc ttcggcctcg tcgtggccga cctcagcttc atctcccttg agcacgtcct
      361 gccccgcatc gccgaacagc tctcgcccga cggtgacgcg gtcgtcctcg tcaagcccca
      421 gttcgaggtc ggccgggacc ggctcggcaa gggcggagtg gtccgctcgg ccggggacag
      481 ggctcgtgcg cttcgggctg tcatcgggta tgccgaggcg gcgcagctcg gcgtgcgagc
      541 catcatggcc agccccatcg acgggtcggc gggcaaccgg gagtatcttc tctgggtgcg
      601 gccttctctg tcggggacgc tgagcaggca ggagctcaac gaccgggtcg atgaggctgt
      661 ccgctcccga ccctgaccag ccgtcccccg gggacgtcgg tgcccagcgc agagaggtgg
      721 cccatgacgc ggcggatcat gctcgtctcg cacccgcggc ggcccgatgt cgcggcggcg
      781 gcgcgcgagg tgacggcacg cttccagcag gccggcatcg aggtgctccc cgccccggac
      841 gaggtctggg cccctgaccg gtccgggatc gagcacctcc tgcccggcgc cggcgcgacg
      901 ggcgcgcgcg actgcgagct cgtctgcgtc ctgggcgggg acggcacgat cctgcgtgga
      961 gccgagctga cccgcgggtc ggagacaccg ctgctcgggg tgaacctcgg tcacgtcggg
     1021 ttcctcgccg aggtcgagcg cgaggacctt gccgccaccg tcgagcacat cgcccgacgc
     1081 cgctacaccg tcgaggagcg gatgaccctc gaggtggtcg ccagtgtcaa cgaccagacc
     1141 atcgcccggt cctgggcgct caacgaggtc tcggtcgaga aggccgcccg cgagcggatg
     1201 atcgaggtcg ccgtcgagat cgacgggcgc ccgctgtcca cgtgggggtg cgacggggtc
     1261 gtcatggcga ctccgaccgg cagcacggct tacgcgttct cggccggcgg gccggtcgtg
     1321 tggcccgatg tcgaggcgat cctgctcgtc ccgaacgctg ctcacgcgct ctttgcgcgc
     1381 ccgctcgtcg tcgggccgaa ctccagactt gccatcgagc tcatcgacga gcgcctcgac
     1441 ggcgatcccg gggtctgcgc cgtcatgtgg tgtgacgggc gtcgctacaa cgatctgccc
     1501 ggcggggcgc gggtcgaggt cactcgaagc gacacgccgg tgcacctggc ccacctggac
     1561 accggaccgt tcacggaccg catcgtggcg aagttcgcgc tgtccgtgca aggatggcgc
     1621 ggcgcggccc gcggccaggc accgcgtggg acctgagacc ggtcgcacgc ccccgttagg
     1681 ctgaccctgt gttccaggag atcaggctgc gggggctcgg tgtgatcgac gacgccgtcc
     1741 tcgagctggg tcccggcctc aacgtcctca ccggagagac cggcgcgggc aagacgatgg
     1801 tggtctcggg tctcggcctg ctgctcggcg gccgggccga ctcgggcctg gtccgcaccg
     1861 gtgactcgat ggcgaccgtc gagggcacga tcgagctgcc cgcgggacac ccggcactgg
     1921 tccgggccca ggacgccggt ggcgataccg acgacgggct ggtcctgctc cgttcggtgt
     1981 ctagcgaggg gcgctcccgg gcccacgtcg gtgggcgcag cgctcccgtc ggcacgctgg
     2041 cccagatcgg ccggctgctc gtcgccgtgc acggtcaggc cgaccagtgg cgactgcgca
     2101 gtagcgcaca gcaccgcgag atcctcgacg acttcggcgg gccgccgctg cgagaggcgc
     2161 tcacggccta cctccggtgt ttcgacgact gggagaccac ccgggccgag gcactgcggc
     2221 tgcgcgctct ggccacggcg agtcgccgcg acgccgagct gctccgggcc gggctcgagt
     2281 acatcgaggc ggtccacccc cagcccgggg aggacctcac cctgcgcgtc gaggacgagc
     2341 ggctcgccca cgccgacggc ctgcgcacca gcgcactgca ggcccaggcg ctgctcgccg
     2401 ggggcgacga cgccgccgag tccccggcgg ggtacggcgc ccccgggggc agcgtccgcg
     2461 agctgctgtc ggcggcgcgg ggggcggttg ccgggatgtc ggtccacgac cccgcgctgc
     2521 gcgagctcga cggacggctg gccgagctcg gctatctcgt cgcggacctc agtgccgatc
     2581 tcgcgcacta cggcgcgcag gtcgaggtcg acccggcccg gctcgcgtgg gtgcagcagc
     2641 gccggtccga gctcgcttcg ctcgtgcgcc ggtatggcga ctccgtcgac gacgtgctgg
     2701 actgggcgag ggagtcggcc gcccggttcg aggagctcgc cggcgcgggg gagcgggccg
     2761 agctgctcga gatcgaggcc accgcgctgc gggccgagct ggcgcggcgc gcggccaccc
     2821 tctcgcaggc ccggtcggca gcggcggccg agctgtccac gcgcatcacc ggcgagctgt
     2881 cccaactcgc gatgaccagc gccgcggtcc acgtcgcggt ccgccagcgc ccggaccccg
     2941 aggggctcag cgtgggcggc gagccggtcc gagccacgcg caccggcatc gac
//
LOCUS       DATAIX010000410         2449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_188645, whole genome shotgun sequence.
ACCESSION   DATAIX010000410 DATAIX010000000
VERSION     DATAIX010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188645"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VHM65_06200"
     CDS             complement(<1..956)
                     /locus_tag="VHM65_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEX2705331.1"
                     /translation="MSVLETISGPDDLKRLAPEQIPALAQEIRDFLVASVSRTGGHLG
                     PNLGVVELTIALHRVFDSPRDTIVFDTGHQSYVHKILTGRRDFSRLRQRGGLSGYPSR
                     TESEHDVVENSHASTALSWADGIATARRLRGERDRHTIAVIGDGALTGGMAWEAINNI
                     AADKDLPLVIVVNDNERSYAPTTGGLAEHLATLRTARSYERILDWGKHALVKTPVVGG
                     AMYHSLHGMKKGIKDILAPQGLFEDLGLKYLGPIDGHDEPAIESALRRASTYGGPVLV
                     HVLTKKGMGYRPARQNEDDRFHAVGVINPETGLPLETVGRSWT"
     gene            1051..2025
                     /locus_tag="VHM65_06205"
     CDS             1051..2025
                     /locus_tag="VHM65_06205"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2705332.1"
                     /translation="MFTAVRTWLRAPAAASAAALALLGAACGSAPGGPTDPTTTGLAL
                     QHLVATRDYLPGLAADLYLPDLVRVAPVVVMVPGGAWVAADRSGLGPLAERLAADGIV
                     VVNATHRPARDGVRFPEPLQDVLCSVGFAVRQAVTAGIEPGPVVVLGHSSGAHLAALA
                     ALAPDRFRGACPYPDMLGDALVGLGGVYDVSELAEVAEPLFGVGPEDDPTAWRDGNPM
                     TWVRQRPSLPVFLGYGDADPLAPAPFTRAFAGALQAGGHPVHVEVVAGADHPGMYDSA
                     YVAEALRAWIAALPAPTTRAGSDTGASVRVSDSVPPTLSHLPRRHGAG"
     gene            2266..>2449
                     /locus_tag="VHM65_06210"
     CDS             2266..>2449
                     /locus_tag="VHM65_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705333.1"
                     /translation="MSTAGPRFFGFVIGGSLPAATAADMVAAGWDQCAFNAVLSPAAA
                     VAEEVAGGWLKDLLGIP"
BASE COUNT          309 a          889 c          848 g          403 t
ORIGIN      
        1 gtccagctgc gcccgacggt ctccaagggc aggccggtct ccgggttgat gacccccacc
       61 gcgtggaagc ggtcgtcctc gttctggcgg gccggccggt agcccatgcc cttcttcgtc
      121 agcacgtgaa ccagcaccgg cccgccatac gtgctggctc ggcgcagcgc cgactcgatg
      181 gcgggctcat cgtgcccgtc gatgggaccg aggtacttca ggccgagatc ctcgaagagc
      241 ccctgggggg cgaggatgtc cttgatgccc ttcttcatgc cgtgcaacga gtggtacatc
      301 gcgccaccga ccacgggggt cttgaccagc gcgtgcttgc cccagtcgag gatgcgctca
      361 tagctgcgcg ccgtccgcag cgtcgccagg tgctcggcga gcccgccggt cgtgggggca
      421 tacgagcgct cgttgtcgtt gacgacgatg acgagcggga ggtccttgtc ggcggcgatg
      481 ttgttgatcg cctcccacgc catccctccg gtgagcgccc cgtcgccgat caccgcgatc
      541 gtatgacggt cacgctcgcc ccgaaggcgg cgcgctgtgg cgatcccgtc ggcccaggac
      601 agtgccgtcg acgcgtgcga gttctcgacg acgtcgtgct cggactccgt gcggcttggg
      661 tagcccgaca gcccgccgcg ctgacgcagc cgcgagaagt cccgacggcc cgtgaggatc
      721 ttgtgcacgt aggactggtg accggtgtcg aacacgatcg tgtcccgcgg ggagtcgaag
      781 acccggtgca gcgcgatagt cagctcgacc acccccaggt tgggcccgag atgcccgccg
      841 gtgcgtgaca ccgaagcgac gaggaagtcg cggatctcct gcgccagggc ggggatctgc
      901 tcgggggcaa gacgtttgag gtcgtcgggg ccgctgatcg tctcaagaac actcacattg
      961 gtcccgttcc ttcctgatgc tcaacggggc gagtctagtt gagccgcgtc cgcagacccc
     1021 agcggcacgg cttcccgcta ggttcgggac atgttcaccg cggtgaggac ctggctccgg
     1081 gccccggccg ccgcgagcgc cgccgccctg gcgctgctcg gcgctgcctg tgggtctgcc
     1141 cccggcggac ccacagaccc cacgaccacg ggccttgccc tccagcatct ggtcgcgacc
     1201 agggactacc tccccggcct cgccgccgac ctctacctgc ccgaccttgt ccgcgtggcc
     1261 cccgtcgtcg tcatggttcc gggcggggcg tgggttgccg ccgaccggtc cggacttggg
     1321 ccgctcgccg agcggctcgc tgccgacggg atcgtcgtcg tcaatgccac tcaccggccg
     1381 gcccgggacg gcgtgcggtt cccggagccc ctgcaggacg tcctgtgctc cgtcggcttt
     1441 gccgtgcggc aggctgtgac tgccggcatc gagcccggac cggtcgtggt gctgggtcac
     1501 tcctccggtg cgcacctcgc ggccctcgcg gccctcgcgc ccgaccggtt ccgaggcgcg
     1561 tgtccctacc ccgacatgct cggcgatgct ctcgtgggcc tcggcggggt gtatgacgtg
     1621 tccgagctcg ccgaggtcgc ggagcctctg ttcggcgtcg gcccggagga tgaccccacg
     1681 gcgtggcgcg acggcaaccc gatgacctgg gtgcgccagc gcccttctct gccggtcttc
     1741 ctcggctacg gtgacgccga cccgctggca ccggcgccgt tcacccgtgc gttcgccggt
     1801 gcgctgcagg ctggcggcca cccggtgcac gtcgaggtcg tggccggggc cgaccacccc
     1861 ggcatgtacg actcggcata cgtcgccgaa gccctcaggg cctggatcgc cgcactgccc
     1921 gcgccgacca ctcgcgcagg ctcggacacc ggcgcgagcg tgcgggtcag cgactccgta
     1981 ccgccgacgt tgtctcacct gccgaggcgc cacggtgcgg gatagcgcgt ccaccctcga
     2041 ccggtgacgg gcgtagcgtg tcttgttgtg gacgacctga gctcgctgct tcaccaggcc
     2101 gcgcagcacg cggccgacta ccgcgcgagc cttccggagc ggtcagtcgc tcccctggcc
     2161 gacgtcggca cactacggaa ggccctgggg ggtcccctcc cgcagaggcg gtccgcaccg
     2221 agcgacgtcc tcgagcagct ggtcgaggcc gccgagcctg gcctcatgtc cactgccggc
     2281 ccaaggttct tcgggttcgt gatcggtggc agcctcccgg ctgcgacagc ggccgacatg
     2341 gtcgcagccg gctgggacca gtgtgccttc aacgcggtgc tctctcctgc agcggccgtg
     2401 gccgaagagg tcgcgggagg ctggctgaag gaccttctgg gaatcccgc
//
LOCUS       DATAIX010000411         1552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_218431, whole genome shotgun sequence.
ACCESSION   DATAIX010000411 DATAIX010000000
VERSION     DATAIX010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218431"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..626)
                     /locus_tag="VHM65_06215"
     CDS             complement(93..626)
                     /locus_tag="VHM65_06215"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX2705334.1"
                     /translation="MSTLLPGHVGTLIVGAGFAGIAMAIRLRAAGDEDWVIIERGPAL
                     GGTWRDNDYPGAACDVQSVLYSYSFAPWPSWTRSYSPRQEIQDYLVHVATERGVGDRV
                     VCDTDLLEARWDDDHGVWRVRTSRGELTAAVLVPPRARWPTRSCPISTGSPTSRGASS
                     TLHAGRPTSRCRALGWP"
     gene            697..>1552
                     /locus_tag="VHM65_06220"
     CDS             697..>1552
                     /locus_tag="VHM65_06220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2705335.1"
                     /translation="MPRLADLEIRPLREEDLDAALRVRTLSFGPLSEGMRSWWEDLNR
                     ELIAQDRAIAVLDGERIVAHARTRPFRQMWGGRLVPMGGVAGVVVDPEYRGRGVASAM
                     VLAAAQQARGHGDVLSALYPTTLPPYRRAGWEVAGTQTRVTVHGDALRRVRGGDVRVR
                     RGTPEDAAAIVALLEARRAGERACGDLVPTAAALAKEIAEDDDAITYVTADGYDGYVV
                     YRWAEDELTVYSFEARTPESARALWALVGSGASVTRRVHIYVRETDVLPYVLDEEAVH
                     DAVVKRWML"
BASE COUNT          219 a          549 c          571 g          213 t
ORIGIN      
        1 tacccagggg gcgctgcgct ggaagacccc gaggtgggcg acgtcgtcga cgatcccggg
       61 cacgacctgg atcgccgagg ctcccgtgcc gatcacggcc acccgagcgc cctgcaccga
      121 gacgtcggcc ggccagcgtg aagtgtggaa gacgcgcccc tggaagtcgg cgagcccgtc
      181 gatatcgggc agcttcgggt cggccagcgc gcccgtggcg ggaccagcac cgctgcggtg
      241 agctcacctc gggaggtgcg cacccgccat accccgtggt cgtcgtccca gcgggcttcc
      301 agcaggtcgg tatcgcagac gacccggtcg cccacgcccc gctcggtcgc cacgtggacg
      361 aggtagtcct ggatctcttg ccggggcgag taggagcgcg tccacgacgg ccagggtgcg
      421 aaggagtagg agtagagcac cgactggacg tcgcatgcgg cgcccggata gtcgttgtcg
      481 cgccacgtgc cgcccagggc ggggccgcgc tcgatgatga cccagtcctc gtcgcccgcc
      541 gcccgcaggc ggatcgccat ggcgatcccg gcgaacccgg caccgacgat gagggtcccg
      601 acgtgccccg gcagcaaggt gctcacggtc gcaccatagc gacgccggga gcaggatgca
      661 ggcaaggtcg gccgcccggg tgactagggt gctggaatgc ctcgacttgc ggacctcgag
      721 atccggcccc tgcgtgagga ggacctcgac gcggccctgc gggtgcgcac gctgtccttc
      781 ggcccgctct ccgagggcat gcgctcgtgg tgggaggacc tcaaccgtga gctcatcgcc
      841 caggaccggg cgatcgcggt cctagacggt gagcgcatcg tcgcgcacgc gaggacccgt
      901 cccttccggc agatgtgggg cggccggctc gtcccgatgg gcggcgtggc cggggttgtc
      961 gtcgacccgg agtaccgcgg ccgcggggtc gcgtcggcca tggtcctcgc cgcggcgcag
     1021 caggcccgag gccacgggga cgtcctcagc gctctctacc cgacgaccct gccaccgtat
     1081 cgccgggcgg ggtgggaggt tgccgggaca cagacccggg tcaccgtcca tggggacgct
     1141 cttcgccggg tgcgcggcgg tgacgtccgc gtgcggcggg gtaccccgga ggacgccgcc
     1201 gccatcgtcg ccctgctcga ggcgcgccgg gccggcgaac gcgcgtgcgg cgatctcgtc
     1261 cccaccgccg cggccctcgc caaggagatc gccgaggatg acgacgcgat cacctacgtc
     1321 acggctgacg ggtatgacgg gtacgtcgtc taccggtggg ccgaggacga gctcaccgtg
     1381 tactccttcg aggcacggac gccggagtcg gcgcgcgcac tctgggcgct cgtcggctct
     1441 ggcgcctcgg tgaccaggcg ggtccacatc tacgtccgag aaaccgacgt attgccctac
     1501 gtcctcgacg aggaggcggt ccacgacgcc gtcgtcaagc gctggatgct cc
//
LOCUS       DATAIX010000412         2133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_52689, whole genome shotgun sequence.
ACCESSION   DATAIX010000412 DATAIX010000000
VERSION     DATAIX010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52689"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1251)
                     /locus_tag="VHM65_06225"
     CDS             complement(118..1251)
                     /locus_tag="VHM65_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2705336.1"
                     /translation="MAVTGATGLLGYQVCRAAAAEGYAVRAVVRGERSAALASLSGGP
                     VEQVRADLDSPASLRAALDGVQLLIHCAARYAFGAQRAQEVHRTNDAGTRAVLEAAAE
                     AGVRRAVVTSSSVTCGSATTPVARTERDHLRTGGLTAEPAPAYYRSKVSQERTALAVG
                     DERNLEVVLALPTVILGGPYARLAPSNAIVLRYLLDPTRSTFPGGCNVVDVRDVAKGH
                     VLLLSEGSPGERYLLGGTDLSWRALHTDVSDLAGRPGPGIEVSPAVAYAVSAAAEAWA
                     RLTGDRPLSTTDEAATVGRWYWYSSARAEDLGYRARPAREAVAHSLAWLLVGEDLPRW
                     ARESLRVDPAVRAARPLVPRPLDATTLRGRPPRRRRSAPRRPR"
     gene            complement(1254..>2133)
                     /locus_tag="VHM65_06230"
     CDS             complement(1254..>2133)
                     /locus_tag="VHM65_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705337.1"
                     /translation="AKDDVARVEVSLGFDPGAHPPQDYAELAEMADGFERAGARAPER
                     APGLRWTMVDEAVVFQTEREVMAPFTELTRRVDVAEGISLMADYLGGRRVPVSADADG
                     RPLWQAERNIYLPQPNWVTAWGGLPIDVCKIELVERDPGRHRLSWRTVRSPNGSATYD
                     DGSLTFEDTGRGTTVLRVAGRQLFALPAAWSAEGLALVPEVREPLVTESYRRFFAVTF
                     DNLEACYEGRPFRVGRAPDGPDTALVTAVVRDLLAMGREGLGAPPENPRGRRSGGRPA
                     PGDVDARGFAHFRGTR"
BASE COUNT          288 a          786 c          809 g          250 t
ORIGIN      
        1 ccgtgtcacc ggcccacgtc accttgcccc ccgggcagga ggcccaggtg cgggtcagcg
       61 ccgaactcac cgacccggcc ttgcgggccg ggcaggtgta tgccgcgacc gtcgaggtca
      121 gcgggggcga cgaggcgctg atcgacgtcg ccgtggcggt cgacccctga gcgtcgtggc
      181 gtcgaggggg cgcgggacga gcggccgggc ggcccgcacc gcagggtcga cgcgcagcga
      241 ctcgcgggcc cagcgcggga ggtcctcgcc gacgagcagc caggccagcg agtgggcgac
      301 ggcctcacgg gcgggacggg cgcggtagcc caggtcctcc gcccgggccg aggagtacca
      361 gtaccaccgg cccacggtcg cggcctcgtc cgtcgtcgac agcggccggt cgccggtgag
      421 ccgggcccag gcctcggccg cggccgacac cgcgtaggcg acggcggggg acacctcgat
      481 gccggggccg ggccggccgg cgagatcgga cacgtcggtg tgcagggctc gccatgacag
      541 gtcggtgccg ccgagcaggt accgctcccc agggctcccc tcggacagga gcagcacatg
      601 gcccttcgcg acgtcccgga cgtcgacgac gttgcaccca ccggggaagg tgctgcgggt
      661 ggggtcgagc aggtaccgca ggacgatcgc gttgctgggg gccaggcggg cgtacggacc
      721 cccgaggatc accgtgggca gcgccaggac gacctcgagg ttgcgctcgt cgccgacggc
      781 cagggccgtc cgctcctggc tcaccttgct ccggtagtac gccggggccg gctccgccgt
      841 gagcccgccg gtgcgcaggt ggtcccgctc ggtccgggca accggagtcg tggccgagcc
      901 gcacgtcacc gacgaggacg tgacgaccgc ccggcgcacg cccgcctcgg cggcggcctc
      961 gaggacggcc cgagtgccgg cgtcgttcgt ccggtggacc tcctgcgccc gctgggcgcc
     1021 gaaggcgtag cgggccgcgc agtggatgag cagctgcacg ccgtcgagtg cggcccgcag
     1081 cgacgccggc gagtcgaggt cggccctgac ctgttcgacg gggccgccgg acagcgacgc
     1141 gagcgcggca gagcgctcac cgcgcacgac ggcccggacc gcgtagccct cggcggcggc
     1201 agcccggcat acctggtatc ccaacagccc ggtggcgccg gtgacagcca ccttcatcga
     1261 gtgccccgga agtgggcgaa gccacgggca tcgacgtcgc cgggtgccgg ccggccgccg
     1321 gatcgccgtc ctcgtgggtt ctccgggggc gcgcccagac cctcgcggcc catggcgagc
     1381 aggtcccgga cgacggcggt gaccagcgcg gtgtccggtc cgtcgggagc ccggccaacc
     1441 cggaagggac ggccttcgta gcacgcttcg aggttgtcga aggtcaccgc gaagaaccgc
     1501 cggtaggact cggtgaccag cggctctcga acctccggga cgagggcgag gccctcggca
     1561 ctccacgccg ccgggagggc gaagagctgg cgccccgcga cgcgcagcac ggtcgtcccc
     1621 cggccggtgt cctcgaacgt gagcgacccg tcgtcatacg tggcggagcc gttggggctg
     1681 cgcaccgtgc gccacgagag ccggtgccgg cccgggtcac gctcgacgag ctcgatcttg
     1741 cagacgtcga tcgggaggcc accccatgcg gtcacccagt tcggctgcgg gaggtagatg
     1801 ttgcgctcgg cctgccagag cggacgcccg tcagcatcgg cgctcacggg gacccggcgc
     1861 ccaccgaggt agtcggccat gaggctgatc ccctcggcga cgtccacgcg gcgggtgagc
     1921 tcggtgaatg gcgccatcac ctcacgctcg gtctggaaca cgacggcttc atcgaccatc
     1981 gtccaccgga gcccaggagc acgctccggc gcccgggcac cggcccgctc gaagccgtcg
     2041 gccatctccg cgagctcggc gtagtcctga ggcgggtggg cccccgggtc gaaccccagg
     2101 ctcacctcga cccgcgccac gtcgtccttg gcc
//
LOCUS       DATAIX010000413         2532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_82863, whole genome shotgun sequence.
ACCESSION   DATAIX010000413 DATAIX010000000
VERSION     DATAIX010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82863"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VHM65_06235"
     CDS             <1..999
                     /locus_tag="VHM65_06235"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2705338.1"
                     /translation="SRWPADVSVQGARVAVIGTGASAIQVVPGIVDDVAHLGVFQRSA
                     PWVLRRGDRPIRPGTRRLYARLPAVYAGVRAATRVSRELNTPVFTESSPIGRVIERMA
                     RAHMQSVVTDPALRAKLTPSYRVGCKRILISDDYYPALTHEHVDVVTDPIERVSATGV
                     LTRDPKSGALVEHPVDVMVLATGFLPSRPPVADKVVGVDGRTLARLWDARGVHAYKGT
                     AVPGYPNLFFLVGPNTGLGHTSMLLMIEAQVAYVAEAVRLVREGTARRVEVTPAATTA
                     WNDELQRRLAKSIWATGGCSSWYVDEHGRNVTLWPGTTWSFERAVKTFDRDAYLTA"
     gene            1047..2246
                     /gene="nadA"
                     /locus_tag="VHM65_06240"
     CDS             1047..2246
                     /gene="nadA"
                     /locus_tag="VHM65_06240"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX2705339.1"
                     /translation="MSISVAPPSPLPLLVLGHGRDLHSERGVECPGDLPAPSDHGLVE
                     RARVAKSVLGDSVFVLGHHYQRDEVIEFADVTGDSFKLAREAAARPDSPYIVFCGVHF
                     MAESADILTAASQTVILPDLAAGCSMADMAGIAQVEDAWDDLVEAGLGDLTVPVTYMN
                     SSAAIKAFTGRHGGTICTSSNARTALAWAFGQTGGVDGRGKVLFMPDQHLGRNTAVRE
                     LGLSLHECVVFDPHRPGGGLTHQQLQDARMILWRGHCSVHGRFSVEAVEAARRAIPGV
                     KVIVHPECRNEVVELADEVGSTEKIITTIAAAPDGSAWVVGTELNLVRRLASQFPRQR
                     IAFLEKNVCYCSTMNRIDLPHLVWVLESLVEGRVVNPIKVDPQVAQAARIALEQMLAL
                     PGRTHRD"
BASE COUNT          359 a          918 c          863 g          392 t
ORIGIN      
        1 tcacgctggc cggccgacgt ctcggtgcag ggcgctcggg tggccgtgat cggcacggga
       61 gcctcggcga tccaggtcgt gcccgggatc gtcgacgacg tcgcccacct cggggtcttc
      121 cagcgcagcg ccccctgggt actgcgccgt ggcgaccggc cgatccggcc cgggacccgg
      181 cgcctttatg ccaggctccc ggcggtctac gccggggtgc gcgcggcgac ccgggtgagc
      241 cgtgagctca atacccccgt cttcaccgag tcgagcccga tcggcagggt catcgagcgg
      301 atggcccgtg cgcacatgca gtccgtggtc accgacccgg ccctgcgcgc gaagctgact
      361 ccgtcatacc gggtcgggtg caagcgcatc ctcatctccg acgactacta ccccgcgctg
      421 acccacgagc acgtcgacgt cgtgaccgac ccgatcgagc gggtcagcgc gactggcgtc
      481 ctcacccggg accccaagtc cggcgccctc gtcgagcacc cggtggacgt catggtgctg
      541 gcgaccggat tcctgcccag ccggcccccg gtcgcggaca aggtcgtcgg tgtcgacggc
      601 cggaccctcg cgcgcctctg ggacgcccgc ggcgtccacg cctacaaggg cacggcagtg
      661 ccgggctacc ccaacctctt cttcctggtc ggccccaaca ccggtctcgg ccacacctcg
      721 atgctgctca tgatcgaggc ccaggtcgcg tatgtcgccg aagccgtccg gctcgtccgt
      781 gaggggacgg cgcgaagggt cgaggtcacc ccggcggcca cgacggcgtg gaacgacgag
      841 ctacagcggc ggctggccaa gagcatctgg gcgacgggcg gctgctcgag ctggtacgtc
      901 gacgagcacg gccgcaacgt caccctctgg ccggggacca cctggtcctt cgagcgggcg
      961 gtcaagacct tcgaccggga cgcctacctc accgcctgag cagcacaccg tccctgcggt
     1021 cttgcggcac gacatacgat ctgcgcgtga gcatttccgt cgcccctccc tctcccctgc
     1081 cgctgctcgt gctcggtcat gggcgggacc ttcactccga gcgcggagtc gagtgccccg
     1141 gcgacctgcc cgcgccgtcc gaccacgggc tcgtcgagcg cgcccgggtc gccaagtcag
     1201 tgctgggtga ctcggtcttc gtgttgggac accactacca gcgcgacgag gtgatcgagt
     1261 tcgccgacgt gacgggtgac tcgttcaagc tggcgcgcga ggcggcggcg cgcccggact
     1321 cgccatacat cgtgttctgc ggtgtgcact tcatggccga gtcggcagac atcctcacgg
     1381 cggcgtcaca gacggtgatc ctgcccgacc tcgccgccgg gtgctcgatg gccgacatgg
     1441 ccgggatcgc gcaggtcgag gatgcgtggg acgacctggt cgaggccggg cttggcgacc
     1501 tgacagtgcc ggtgacctac atgaactcct cggcggccat caaggcgttc accggccggc
     1561 atggtggcac gatctgcacc tcctcgaacg cccgcacggc gctggcctgg gccttcggcc
     1621 agaccggcgg ggtggacggg cgcggcaagg tcctctttat gcccgaccag cacctcgggc
     1681 gcaacacggc cgtgcgcgag ctcgggctgt cgctgcacga gtgtgtcgtc ttcgacccgc
     1741 accgccctgg gggcgggctg acccaccagc agctgcagga cgcccggatg atcctgtggc
     1801 gagggcactg ctcggtgcat gggcggttct cggtcgaggc cgtcgaggcg gcccgccggg
     1861 cgatccctgg ggtcaaggtc atcgtgcacc ccgagtgccg caacgaggtg gtcgagctgg
     1921 ccgacgaggt cgggtcgacg gagaagatca tcaccaccat cgccgcagct cccgacgggt
     1981 cggcctgggt cgtcggcacc gagctcaacc tcgtccgccg gctcgcgtcc cagttcccga
     2041 ggcagcgcat cgccttcctg gagaagaacg tctgctattg ctcgacgatg aaccggatcg
     2101 acctgccgca cctcgtctgg gtgctcgagt ccctcgtcga agggcgcgtg gtcaacccga
     2161 tcaaggttga cccccaggtc gcccaggcag cccgcatcgc gctggagcag atgctcgccc
     2221 tgcccggcag gactcaccgg gactgaccgg cgggactcag ccaccggcga cccggcccag
     2281 gctccatttc gctcggaccc aggctccatt tcgctgcgta tgccgtgccc gcttctggcg
     2341 ggcagggcgt gcaagcaggg tcgggtgccg cagccacgcc accaccggtg accccgcacc
     2401 cacgttccgg aggcgaacca caccctgtgc gtgagttccg gtcacgacgc cgtttctgca
     2461 gctgtgccgt cgtgaccgga actcacgcac agggtgtggt tcgcctccgg aacgtgggtg
     2521 cggggtcacc gg
//
LOCUS       DATAIX010000414         2712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_112521, whole genome shotgun sequence.
ACCESSION   DATAIX010000414 DATAIX010000000
VERSION     DATAIX010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112521"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..2469
                     /gene="sulP"
                     /locus_tag="VHM65_06245"
     CDS             205..2469
                     /gene="sulP"
                     /locus_tag="VHM65_06245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HEX2705340.1"
                     /translation="MAKKKPGKILGKEGSAEVQAMSDVAVPQTAAPTTPELPLIERRQ
                     ALEYQTSVRREHFLVRAFVSATPGTDIFKHYRKHWLRPDILAGVAVAAYLVPQCMAYS
                     AIVDVPPVVGLWAALTALIGYAFMGGSRILSVGPESTVALMAGTAIAPLVAQQPDKAI
                     AFGAALSLIVGGWCFVARMARLGVIAELLSQPLLVGYLAGAAVLMVVGQLGKMTGTHV
                     DGETIVAQVISFAGVVRDTHLTTVIVGFGTFALLIAIHLVKPRWPAPLIAVAVATIVC
                     TVMNLKDLGVKVVGEVPTGLPLPSLPAVSWSDLETLLLAGIGVAIVAYGDNTLIARGF
                     HATTDPDEDRSVNEIDPQAELVALGGVSIVVGLFGGYPVSSSGSRTALAVASGARTQV
                     YSLTAAVCVVVVLFAAGPLVANLPAASLGAVVFYAASKLVSWKETKRLAKFRPTELYL
                     AAAAALGTVLFGILAGVGIAIALSVLEMSQRLARPHDAVLGRVPDIPGMHDVADYPNA
                     QTLPGLIVYRYDAPLFFANVGDLRRRSLLVVDQENASFPDSPAKWFILNVEANVEVDI
                     TAADGLRELHQDLAERGIRFGLARVKHDLLIPLSKAGLTELIGLDMLFPTLPVAEEAY
                     LTWAGQQQLEQQRQAEEERLAQEQARELAAQQAALAGMEAETGTGSEEAVVAPAARAP
                     DLWRLRIAQSEDVANSHRRTHGHAALTPEVVTPPFSTGAPEVAAGPPSATNGAPPPAH
                     EPAEPVVTDGPQHA"
BASE COUNT          379 a          972 c          915 g          446 t
ORIGIN      
        1 ctcttgcggg ctatgggtac ccctgagcgc tcggtgactt cggcgccctt ctctcgtggg
       61 tgcactctgt ccctcgcgct gcggggagcg gtatcttcat ggccaacacg gtgtgaacgt
      121 caggtaaacc gatgtggccc ccagattgcc ccaacagggt cgacaagtcc gagccggctg
      181 cggccaacag gcaatggagt caggatggcg aagaagaagc ccggcaagat tctgggcaag
      241 gaaggctccg ccgaagtcca agccatgtcc gacgtcgccg tgccgcagac ggcggcgccc
      301 accacgcccg agctgccgct catcgagcgc cgccaggcgc tggagtacca gacgagcgta
      361 aggcgcgagc acttcctggt ccgcgccttc gtgtcggcca cgccgggcac cgacatcttc
      421 aagcactacc gcaagcactg gctgcgtccg gacatcctcg ccggggtcgc cgtcgcggca
      481 tacctggtcc cgcagtgcat ggcctactcg gccatcgtcg acgtgccacc ggtcgtcggc
      541 ctgtgggcgg ccctcaccgc cctcatcggg tacgccttca tgggtgggtc ccgcatcttg
      601 tcggtcggcc cggagtcgac ggtcgccctg atggccggca cggcgatcgc gccactggtc
      661 gcccagcagc ccgacaaggc gatcgccttc ggcgcggcac tcagcctcat cgtcggtggc
      721 tggtgcttcg tcgcccggat ggcccggctc ggggtcatag ccgagctgct ctcccagccg
      781 ctgctcgtcg gctatctcgc cggcgccgca gtcctcatgg tcgtcggcca gctcggcaag
      841 atgaccggca cccacgtcga cggcgagacg atcgtcgccc aggtcatctc cttcgccggt
      901 gtcgtcaggg acacccacct caccaccgtc atcgtcggtt tcgggacatt cgcgttgctc
      961 atcgccatcc atctggtcaa gccgcgctgg ccggccccgc tcatcgcggt tgccgtggcc
     1021 accatcgtct gcacggtgat gaacctcaag gaccttggcg tcaaggtcgt cggcgaggtc
     1081 ccgaccggtc tgccgctgcc atcgctgccc gccgtctcgt ggagcgacct cgagaccctg
     1141 ctcctggcgg ggatcggcgt ggcgatcgtc gcgtatggcg acaacaccct catcgcgcgg
     1201 gggttccacg ctacgacaga ccccgacgag gaccgcagtg tcaacgagat cgacccccag
     1261 gccgagctcg tcgccctcgg tggggtgagc atcgtggtgg gtctcttcgg tggctacccc
     1321 gtctcgtcgt ccggctcacg cacggctctg gccgtcgcct caggcgcccg tacgcaggtc
     1381 tactcgctca ccgcggcggt ctgtgtcgtc gtcgtgctct tcgcggccgg cccgctcgtc
     1441 gcgaacctcc ccgccgcctc gctgggtgcc gtcgtcttct acgccgcgag caagctcgtg
     1501 tcgtggaagg agaccaagcg gctggccaag ttccgtccca ccgagctcta cctcgccgcc
     1561 gcggcggcgc tcgggacggt cctcttcggg atcctcgccg gcgtcgggat cgcgatcgcg
     1621 ctcagcgtcc tggagatgtc ccagcgcctg gcccggccgc acgacgcggt cctcggccgg
     1681 gtgccggaca tcccgggcat gcacgacgtt gctgactacc ccaacgcgca gaccctgccc
     1741 gggctcatcg tctaccggta tgacgcgcct ctcttcttcg ccaacgtcgg ggacctgcgc
     1801 cgtcggtcgc tgctcgtcgt ggaccaggag aacgcatcct tcccggactc tccggccaag
     1861 tggttcatcc tcaacgtcga ggccaacgtc gaggtcgaca tcaccgcggc cgacggtctg
     1921 cgcgaactgc atcaggatct cgccgagcga gggatccgct tcggtctggc gcgggtcaag
     1981 cacgatctgc tcatcccact gagcaaggcc ggtctgaccg agctcatcgg gctcgacatg
     2041 ctgttcccca ccctgccggt ggcggaggag gcctacctca cgtgggccgg tcagcaacag
     2101 ctcgagcagc agcgacaggc cgaggaggag cggctcgcgc aggagcaggc gcgtgagctc
     2161 gcggcgcagc aggcggccct ggcggggatg gaggccgaga cgggcaccgg ctctgaggag
     2221 gccgtcgtcg ccccggcagc gcgggcaccc gacctgtggc gcctgcggat cgcccagtcc
     2281 gaagacgtgg ccaacagtca caggcgcacc cacggacacg ccgccctcac ccccgaggta
     2341 gtgacccctc cgttctcgac aggtgcaccc gaggtcgcgg ctgggccgcc gagcgccacg
     2401 aacggcgcgc caccacccgc tcacgagccg gccgagcccg ttgtgaccga tgggcctcag
     2461 cacgcttagg agggggcgtc tgggactctg ttgctggcgg ggtgcgtgtt acccgtggtg
     2521 aggtggttcc ggccggtcag ggccggtcag atcggcctcg gtagcccggg ggcggctcac
     2581 tgaggtggtt ccggccgatc agggccggcc agatcggcct cggtagtgcg ggggcgggtc
     2641 gttgaggtgg ttccggccgg tcagggccgg ccagatcggc ctcggtagcc cggggacggc
     2701 tcactgaggt gg
//
LOCUS       DATAIX010000415         4380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_22602, whole genome shotgun sequence.
ACCESSION   DATAIX010000415 DATAIX010000000
VERSION     DATAIX010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22602"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VHM65_06250"
     CDS             <1..735
                     /locus_tag="VHM65_06250"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX2705341.1"
                     /translation="AARVDDAGLRVSSLCRGGFLTATDNAGARAALEDNRAAVVEAAT
                     LGTAELIMVVGGLPAASAPGGPALPGGDRDLVGARRRVADRIGDLVPFALEHGVRLVL
                     EPMHPMFAADRGVISTLGQALDLAAPHPPQAVGVVVDTYHVWWDPELEAQIARAGAEG
                     RLASYQVCDWILPLAADPLLSRGYMGDGYVDFGSIGRWVTAAGYTGDVEVEIFNQDVW
                     DTPGDEVLAIITDRYVRHVLPSLTPH"
     gene            complement(879..1655)
                     /locus_tag="VHM65_06255"
     CDS             complement(879..1655)
                     /locus_tag="VHM65_06255"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2705342.1"
                     /translation="MTGATPSPAGVPPFPGGTSVSHLRVYDWPTPDGAGLAGGGTPHL
                     HTASSEGYVVLAGAGSVQTLSPQGFAETALVPGVVVWFTPGVVHRLVNDGGLEILVVM
                     SNAGLPEAGDAVLTFPADVLADVGRYQKAATLPAPGPRPGAENADDDVVVAAAARTRR
                     DLALEGFATLRARLEHEGPSGLDDLYAAAGRLVADRVPGWQEIWSAVVPHQTAATAQA
                     LTDLAAGNAAHLAQAAVGSATARPGPRRYGLCGRLQTWPL"
     gene            complement(1652..2599)
                     /locus_tag="VHM65_06260"
     CDS             complement(1652..2599)
                     /locus_tag="VHM65_06260"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HEX2705343.1"
                     /translation="MTARRTRLTAWHDIGWSVQVAHHGQDLLTYVYAPDDVQLESPRP
                     YFHPLYTLGGDLVSLFRPHDHLWHKGIAWSLPDLGPHNFWGGPNYVHGQGYVQLDNNG
                     SMDHQRFTALDVTDDRVQVSHQLVWHAQPAAGQEDGAEIVHEARAFAVTVPTHDAWVL
                     TFSSAITNVCGEQLDIGSPTTKGRENAGYGGLFWRGPRSFTGGTIIAPGYAGGEEIRG
                     TRAEWMGFTGQHDQTARHSSLVMVDDTANPQHPPQWFMRNEWFAGVNPAPFFSEEVPF
                     GAGQTLQFRYAVVIAEGQADAQRGEALAGLGRAALQEWG"
     gene            complement(2596..3735)
                     /locus_tag="VHM65_06265"
     CDS             complement(2596..3735)
                     /locus_tag="VHM65_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2705344.1"
                     /translation="MSLRRTLIVGTGTIAGAHAAAVAAHGSRAVVVACVDLEPARAQD
                     FAARHGIEGWSADLPTALRQTWPDGGRVDLAHICTPPGSHVPLAVQCLEAGVPVLLEK
                     PPALSLAEADKLIAVSERTGVGVAVVFQHRFGSGARRAHALLVDGARSDAGIGRPLLA
                     TCHTLWYRDADYFAVPWRGRWDTEGGGPTMGHGIHQFDLLLALLGPWTEVTAFAGRQA
                     RDTDTEDVSMAVVRFASGALATVVNSVVSPRQTSSLRIDTERATLEVEHLYGYNDADW
                     TFTAAPGHEDLAALWTTDDTEPRSSHASQMAALLDAMDAGEQLPVPLSEARSTLELVA
                     AIYASAFTGQVVLRGDIGPGHPFYARMDGTGAPWTTTLDNEEDLA"
     gene            complement(3732..>4380)
                     /locus_tag="VHM65_06270"
     CDS             complement(3732..>4380)
                     /locus_tag="VHM65_06270"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2705345.1"
                     /translation="GMGPRPARGTGNPHDPWALDLTAEPRLKRLYLDAEHYDGYLRDR
                     DVFAPGITIEDNMAVLVDYRGGATLSYSLNAHAPWEGYKVTVNGTAGRAELEVVERGS
                     VEFDEHGRSILDPSATPEAWAGDTLRPFGDRLLVQKHWERATSHPIPTRVGGHGGGDA
                     ILLADVFRGPGEDPLGRPAGYVDGVRAVAVGIAANRSMATRQAVEVADLDLGIGL"
BASE COUNT          594 a         1577 c         1584 g          625 t
ORIGIN      
        1 gcggcacgag tggacgacgc cggcctgcgc gtctcgtcgt tgtgccgagg gggcttcctc
       61 acggccaccg acaacgccgg ggcgagggcc gcgctggagg acaaccgggc cgccgtggtc
      121 gaggccgcga cgctgggtac cgccgagctg atcatggtgg tcggcgggtt gcccgctgcg
      181 agcgcaccgg gtgggcccgc gctcccgggc ggggatcgcg acctggtcgg cgcacgtcgg
      241 cgcgtggccg accgcatcgg tgacctcgtg ccgttcgcgc tcgagcacgg cgtgcgcctc
      301 gtgctcgagc ccatgcaccc gatgttcgcc gcggaccgcg gcgtgatctc caccctcggc
      361 caggcgctcg acctggccgc gccccaccca ccacaagcgg tcggcgtcgt cgtcgacacc
      421 taccacgtgt ggtgggaccc cgagctcgag gcccagatcg cccgggccgg cgcggaaggt
      481 cggctggcgt cctaccaggt atgcgactgg atcctgccgc tagccgccga cccgctgctg
      541 tcgcgcggct acatggggga cgggtacgtg gacttcgggt cgatcgggcg ctgggtgacg
      601 gccgccgggt acaccggtga cgtcgaggtc gagatcttca accaggacgt ctgggacaca
      661 cccggggacg aggtcctggc gatcatcacc gatcgctacg tgcgtcacgt cctgcccagc
      721 ctcactcccc actgatggcg ggtcagtaga tgttgcgatg ctcgcggaac tcgcgccaga
      781 cgttctcgaa gaagtcatcg gatcctctgt ccctgcgcgc cgaggtggag gacccgcgcc
      841 ccagcccctg gcctgccgct cgagtggcag gccaggtctc acaggggcca ggtctgcagg
      901 cggccgcaca ggccgtaacg acgtgggccg gggcgggccg tcgcgcttcc taccgccgcc
      961 tgcgccaggt gcgctgcgtt tcccgcggcc aggtcggtga gcgcctgcgc cgtcgcagcg
     1021 gtctggtgcg gcacgacggc cgaccagatc tcctgccacc cgggcacccg gtccgcgacg
     1081 agccgcccgg ccgcggcgta caggtcgtcc agaccggacg gcccctcgtg ctccagccgg
     1141 gcgcgcagcg tcgcgaaccc ctccagggcc aggtcccgcc gggtcctcgc cgcagccgca
     1201 acgacgacgt cgtcgtccgc gttctccgca cccgggcgag gcccaggcgc aggcaacgtg
     1261 gccgccttct gatagcgccc gacatcggcc agcacgtccg cagggaacgt gagcaccgcg
     1321 tcacccgcct cgggaagtcc cgcgttggac atgacgacga ggatctccaa cccaccgtcg
     1381 ttgacaaggc ggtgcaccac ccccggtgtg aaccacacga ccacgccggg caccagcgcg
     1441 gtctcggcga acccttgagg actgagggtc tgcacgctcc cggcaccggc cagcactacg
     1501 tatccctcgg atgatgcggt gtgcaggtgg ggcgtgccgc cgcccgcgag gccggcaccg
     1561 tccggcgtcg gccagtcgta gacgcgcagg tggctgaccg acgtgccgcc ggggaacggt
     1621 ggcacgccgg ccggactggg cgtcgccccg gtcatcccca ctcctgcaga gcggcgcggc
     1681 ccagcccggc cagcgcctcg ccccgctggg cgtcggcctg accttcggcg atgaccacgg
     1741 cgtaccggaa ctgaagcgtc tgaccggcgc cgaacggcac ctcctcgctg aagaacggtg
     1801 cagggttcac tccggcgaac cactcgttgc gcatgaacca ctgcgggggg tgctgcgggt
     1861 tggcggtgtc gtccaccatg accaggctcg agtggcgcgc ggtctggtca tgctggccgg
     1921 tgaagcccat ccactcggcg cgggtaccgc ggatctcctc ccctcccgca tacccggggg
     1981 cgatgatcgt gccgccggtg aaggaccgcg gcccgcgcca gaacaatcct ccgtacccgg
     2041 cgttctcgcg gcccttggtg gtcggcgacc cgatgtccag ctgctcaccg cagacgttgg
     2101 tgatcgccga ggagaacgtc agcacccagg cgtcgtgggt gggtacggtc accgcgaagg
     2161 cacgcgcctc gtgcacgatc tccgccccgt cctcttggcc tgcggcgggt tgcgcgtgcc
     2221 agaccagctg gtgggagacc tggacccggt cgtcggtcac gtcgagggca gtgaaccgct
     2281 ggtggtccat cgacccgttg ttgtccagct gcacgtaccc ctggccgtgg acatagttgg
     2341 gcccgcccca gaagttgtgg ggtccgaggt ccggcaagga ccaggcaatc cccttgtgcc
     2401 aaaggtggtc gtgcggacgg aacaggctga ccaggtcgcc gccgagggtg tacagcgggt
     2461 ggaagtaggg ccgcggcgac tccagctgca cgtcgtccgg ggcgtagacg taggtgagca
     2521 ggtcctgccc gtggtgggcg acctgcaccg accagccgat gtcgtgccaa gcggtcaggc
     2581 gggtgcggcg tgcggtcacg cgaggtcctc ctcattgtcc agcgtggtgg tccagggtgc
     2641 cccggtgccg tccatgcgcg cgtagaaagg gtggcccggg ccgatgtccc cgcgcaggac
     2701 cacctgcccg gtgaacgccg aggcgtagat cgccgcgacc agctccaggg tgctgcgcgc
     2761 ctcgctgagc ggcacgggca gctgctcgcc ggcgtccatc gcatcgagca acgccgccat
     2821 ctggctggcg tggctgctgc gcggttcggt gtcgtcggtg gtccaaagag cggcgaggtc
     2881 ctcgtggccg ggcgcagcgg tgaaggtcca gtcggcgtcg ttgtagccgt acaggtgctc
     2941 gacctcgagc gtcgcgcgct cggtgtcgat gcgcagcgag gatgtctgtc gcggcgacac
     3001 caccgagttc acgaccgtcg cgagcgcccc gctggcgaat cgcaccacgg ccatggacac
     3061 gtcctcggtg tcggtgtccc gcgcctgccg tccggcaaac gcggtgacct ccgtccacgg
     3121 gccgagcagc gcgagcagca ggtcgaactg gtggatgccg tggcccatcg tcggcccgcc
     3181 gccctcggtg tcccaccgtc cacgccaggg cactgcgaag tagtcggcgt cgcggtacca
     3241 gagggtgtgg cacgtggcca ggagcggccg cccgatgccc gcgtccgacc gcgcgccgtc
     3301 gacgagcagt gcgtgtgccc ggcgcgcccc tgagccgaac cggtgctgga agacgacggc
     3361 gacgcccacc ccggtgcgct cggagacggc gatcagcttg tcggcctccg caaggctcag
     3421 cgccggcggc ttttccagga gcaccggcac accggcctcg aggcactgga cggccaatgg
     3481 gacgtgcgag ccgggtgggg tgcagatgtg cgcgaggtcc acccggccgc cgtcgggcca
     3541 ggtctgccgc agcgcggtgg ggaggtcggc gctccagccc tcgatcccgt gtcgggcggc
     3601 gaagtcctgg gcccgcgccg gctcgaggtc gacgcacgca acgaccaccg cccgcgaacc
     3661 gtgtgcggcg accgcggcgg cgtgcgcgcc cgcgatcgtc cccgtgccga cgatcagcgt
     3721 gcgccgcagg ctcacagccc gatcccgagg tcgaggtcgg cgacctcgac cgcctgcctg
     3781 gtcgccatcg acctgttcgc ggcgatcccc actgcgacgg cgcgcacacc gtcgacgtac
     3841 ccggcggggc gaccgagcgg gtcctcaccg gggccgcgga agacgtcggc gagcaggatc
     3901 gcgtcgccac caccgtggcc accgacgcgc gtggggatcg ggtgcgatgt cgcccgctcc
     3961 cagtgcttct gcaccagcag ccggtccccg aaggggcgca gcgtgtcccc ggcccatgcc
     4021 tcgggcgtcg cactcgggtc caggatggac cggccgtgct cgtcgaactc gaccgaaccg
     4081 cgctcgacga cctcgagctc ggcgcgaccg gccgtgccgt tgaccgtgac cttgtagccc
     4141 tcccacgggg catgggcgtt cagcgagtag ctcagggtcg cgccgccgcg atagtccacg
     4201 agcacggcca tgttgtcctc gatggtgatg ccgggcgcga agacgtcccg gtcgcgcagg
     4261 tagccgtcgt agtgctcggc gtcgaggtac aggcgcttga ggcgcggttc cgcggtcagg
     4321 tccagcgccc acgggtcgtg cgggttgccc gtgccgcggg ccgggcgagg gcccatcccc
//
LOCUS       DATAIX010000416         3280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_112631, whole genome shotgun sequence.
ACCESSION   DATAIX010000416 DATAIX010000000
VERSION     DATAIX010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112631"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VHM65_06275"
     CDS             complement(<1..377)
                     /locus_tag="VHM65_06275"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEX2705346.1"
                     /translation="MRTHGDVEARLRAAHLRVTAPRRAVSDRNSLSLSPNGPLLLHDH
                     HLVDTLAHFNRENVPERRPHAKGAGAFGEFEVTGDVSAYTKAAVFQPGTRSRMLARFS
                     TVAGELGSPDTWRDVRGFSLRWYT"
     gene            complement(481..1470)
                     /locus_tag="VHM65_06280"
     CDS             complement(481..1470)
                     /locus_tag="VHM65_06280"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX2705347.1"
                     /translation="MGSLRRIKVATIILVAITVVGTIGYMLLGFTVVEAMYQTITTLA
                     TVGFREVRELSTVGQVFTMMLIVTGVGAVLYNIGVVFEALTEGHLRTDLERRRMDRQI
                     AALSGHVIICGNGRVGRAAKNYLTASGQDVVVVDIVPARVEHLRLPYIIGDCTRDDVL
                     KAAGIARARALIAALETDADTVYVTLSARALRPDLVIVSRARTTDSKDKLVLAGATRA
                     VNPQRIGGLRLAAFAMQPDVAEFLDVVMHDEELDLRIQEVHVPPTSALVGRTLGDIRV
                     TERTGGMLLAMRTAPGVPFIPSPPPATEVPAGAVLIAFGTLAQVDALRHLARA"
     gene            1693..2487
                     /gene="infC"
                     /locus_tag="VHM65_06285"
     CDS             1693..2487
                     /gene="infC"
                     /locus_tag="VHM65_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX2705348.1"
                     /translation="MPSRLARHTGSGFSGSSAHGVWSLCRCWTAASGASVTRPSHRPK
                     EHHISEPRINDRIRVPEVRLVGPNGEQVGIVRVEDALRLAAEADLDLVEVAPLAKPPV
                     AKLMDFGKWKYEAAIKEREARKNQINTVIKEIKLRPKIDPHDYGTKKGHVERFLKAGD
                     KVKVTIMFRGREQSRPELGYRLLQKLATDIGELGFVESAPKQDGRNMIMVLGPTRKKA
                     EARAEQRRRREGSAEAPSTDERAPEAPDSAVDAALVAEVPADTAVS"
     gene            2550..2744
                     /gene="rpmI"
                     /locus_tag="VHM65_06290"
     CDS             2550..2744
                     /gene="rpmI"
                     /locus_tag="VHM65_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX2705349.1"
                     /translation="MPKMKTHSGTKKRVRVTGSGKLMREQAGGRHRLEVKSSKEMRSI
                     SGDIPLSGPHAKKARKLLGR"
     gene            2833..3210
                     /gene="rplT"
                     /locus_tag="VHM65_06295"
     CDS             2833..3210
                     /gene="rplT"
                     /locus_tag="VHM65_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX2705350.1"
                     /translation="MARVKRAVNAHKKRRVVLERASGYRGQRGRLYRKAKEQVTHSLG
                     YAYRDRRARKGDFRRLWIQRINAAARANGMTYNRFIQGLKASGVEVDRKILADLAVTD
                     ASAFTVLVELSRANVPAHDAPAA"
BASE COUNT          570 a         1139 c         1135 g          436 t
ORIGIN      
        1 gtgtaccacc gcagcgagaa cccgcgcacg tcgcgccagg tgtcggggga gccgagctcg
       61 cccgccaccg tcgagaagcg cgcgagcatg cggctcctcg tgcccggctg gaacaccgct
      121 gccttcgtgt atgccgacac gtcaccggtg acctcgaact caccgaaggc gccggcgccc
      181 ttggcgtgtg gccggcgctc ggggacgttc tcgcggttga agtgcgcgag ggtgtccacg
      241 aggtggtggt cgtgcaggag cagcggaccg ttgggggaga gcgagaggct gttgcgatcg
      301 ctgacggcgc ggcgcggggc cgtgacgcgc aggtgtgccg cgcgcagccg cgcttcgaca
      361 tcaccgtgtg tccgcaccct ctcaaccaac cacctgatct ggaatcagtc aagagaaggg
      421 cggtgcaccc cttcgtggcc ggagctgggc ttcttgctcg cgcgtcaggg tgggccgcgc
      481 tcaggcgcgc gcgaggtggc gcaaggcgtc gacctgagcc agggtcccga aggcgatgag
      541 gaccgccccg gccggcacct ccgtggcggg cggagggctg gggatgaacg gcacaccagg
      601 cgcggtgcgc atcgccagaa gcatccctcc cgttcgctcg gtgacccgga tgtcgccgag
      661 cgtgcggccg accaaggccg aggtgggcgg cacgtggacc tcctggatcc gcaggtccag
      721 ctcctcgtcg tgcatgacga cgtcgaggaa ctcggccacg tccggctgca tcgcgaacgc
      781 ggcgaggcga aggccgccga tgcgctgcgg gttgaccgcg cgggtcgcgc cggcgaggac
      841 gagcttgtcc ttggaatcgg tcgtcctggc ccgcgagacg atgacgaggt cggggcgcag
      901 ggcgcgggcc gacagggtca cgtagaccgt gtcggcgtcg gtctccagcg cggcgatcag
      961 ggcgcgggcg cgcgcgatgc cggccgcctt gaggacgtcg tcacgagtgc agtcaccgat
     1021 gatgtacggc agcctgaggt gctcgacccg cgcggggacg atatcgacga cgaccacgtc
     1081 ctgacccgac gcagtgaggt agttcttcgc cgcgcggccg acccgcccgt tgccgcagat
     1141 gatgacgtgc ccgctcaggg cagcgatctg ccggtccatg cgtcgcctct ccagatccgt
     1201 gcgcaggtgg ccctcggtca gcgcctcgaa gacgacgccg atgttgtaca gcaccgcacc
     1261 gaccccggtg acgatgagca tcatggtgaa cacctgcccg acggtgctga gctcgcggac
     1321 ctcgcggaac ccgaccgtcg ccagcgtcgt gatcgtctgg tacatcgcct cgacgacggt
     1381 gaatcccagc aacatgtagc cgatggtccc gacgacggtg atggcgacca ggatgatcgt
     1441 cgccaccttg atccgccgca ggctacccac ggcgctcacc caaccgcatc cacggccgtc
     1501 gcgccatact tccctggtag tcttgtcggc gaccacctgt gtctcacgca agcgcctgcg
     1561 cgtgcgtgag ttctcagggt gcaagaggag cgccagttcc cacccgcgtc gacgagttcg
     1621 gtcgccgggt ccaaggtccg gcacggatgg cgtggccctc gcttcgacgg gggacacgct
     1681 gcacggcctc gtatgccgag ccggctggct cgccataccg gatctgggtt ctcgggctcc
     1741 agcgcacacg gcgtgtggag cctttgtcgt tgctggacag cggcctcggg cgcctcggtc
     1801 accaggccta gccaccgacc taaggagcac cacatcagcg agcctcgcat caacgaccgc
     1861 atccgggtcc cggaagtgcg gttggtcggc cccaatggcg agcaggtcgg catcgtgcgt
     1921 gtcgaagacg cgctgcgcct ggccgcggag gcagacctcg acctcgtcga ggtggccccg
     1981 ttggccaagc cgccggtcgc caagctcatg gatttcggca agtggaagta cgaagccgcg
     2041 atcaaggagc gggaagcccg caagaaccag atcaacacgg tcatcaagga gatcaagctc
     2101 cgcccgaaga tcgacccgca cgactacggc accaagaagg gccatgtcga gcggttcctc
     2161 aaggcgggcg acaaggtcaa ggtgacgatc atgttccgcg gtcgcgagca gtcccggccc
     2221 gagctgggct accggctgct gcagaagctg gccaccgaca tcggcgagct cggcttcgtc
     2281 gagtccgccc cgaagcagga cggccgcaac atgatcatgg ttctcggccc gacccgcaag
     2341 aaggccgagg ccagggccga gcagcgccgt cgccgcgagg gctcggccga ggcgccgagc
     2401 acggacgaga gggcccctga ggcgccggac agtgcggtcg atgcggctct ggttgccgag
     2461 gtcccggccg acaccgccgt cagctagcca gcacagcacc gaccgaaggc caccgaccga
     2521 acacccacga cgaaggagaa cggcccacca tgccgaagat gaagacgcac agcgggacca
     2581 agaagcgggt ccgggtcacc ggctccggca agctgatgcg cgaacaggcc ggcggacggc
     2641 accgtctcga ggtgaagtcg agcaaggaga tgcgctcgat ctcgggcgac atcccgctgt
     2701 ccggcccgca cgccaagaag gccaggaagc tcctcggccg ctgagccgca ggcgccaccg
     2761 cacgcacggc acgacccacc agctcacagc gcgcccgggg agagggcgtc atgacagaca
     2821 aggagaactc acgtggcacg cgtgaaacgg gcggtcaacg cccacaagaa gcgccgggtc
     2881 gtcctcgagc gcgccagcgg ctaccgcggc cagcgcggac ggctctaccg caaggccaag
     2941 gagcaggtca cccactccct cggctacgcc taccgggacc gcagggccag gaagggcgac
     3001 ttccgtcgcc tgtggatcca gcggatcaac gccgcggccc gcgccaacgg catgacctac
     3061 aaccggttca tccagggcct caaggcttcg ggcgtcgagg tcgaccgcaa gatcctcgcc
     3121 gacctcgcag tcaccgacgc ctcggccttc accgtgctcg tcgagctctc ccgggccaac
     3181 gtcccggccc acgacgcacc ggccgcctga ggcggcgaac gcctgacgcg ccgcgcgctg
     3241 cggcaggagc cccatgctga ccaatcctcg cgccgagcga
//
LOCUS       DATAIX010000417         1739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_248363, whole genome shotgun sequence.
ACCESSION   DATAIX010000417 DATAIX010000000
VERSION     DATAIX010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248363"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..733
                     /locus_tag="VHM65_06300"
     CDS             146..733
                     /locus_tag="VHM65_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2705351.1"
                     /translation="MLVKAIRNLVGNAIAYSESGTQVTVKVRPSGSFVEVSVSDEGQG
                     ISPTDQERIFERFYRVDAARSRATGGTGLGLAIVKHVCVNHGGAVSVWSQVGAGSTFT
                     IRLPGASLPPGEPAPSRHTHPMTDRSAHWYGGPDPSSSARPADPATAVDATPGTASPF
                     RDPAPAAGSRRVPVGARTRTAGPRSPQRHSREATL"
     gene            730..1410
                     /locus_tag="VHM65_06305"
     CDS             730..1410
                     /locus_tag="VHM65_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2705352.1"
                     /translation="MSRILVVEDEESFSDPLSYLLRKEGYEVAVADTGPAALAEFDKH
                     GADLVLLDLMLPGISGTEVCRTLRQRSAVPIIMLTAKDSEIDKVVGLEIGADDYVTKP
                     YSSRELLARIKAVLRRLAEPEDPLPATIEAGPVRMDVERHIVTVKGAPVPLPLKEFEL
                     LEMLLRNSGRVMTRVQLIDRVWGSDYVGDTKTLDVHIKRLRAKVEPDPGNPRHILTVR
                     GLGYKFEA"
     gene            complement(1485..>1739)
                     /locus_tag="VHM65_06310"
     CDS             complement(1485..>1739)
                     /locus_tag="VHM65_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705353.1"
                     /translation="NSGDGATRVAFSSAGAPGGSVMVPPGGTAPLTSVTIPQIGAAPG
                     AVVSLVVSTPTSGDVTVQVPVLPPTLYYSTVTPSPVAAAP"
BASE COUNT          263 a          645 c          600 g          231 t
ORIGIN      
        1 gccttgacga gcagctcctt gtcgccgtag acgacgcagt gcggcgctgg aggcgatcga
       61 gcgctcccac ctggaggccg aggcccagga catcagcctg atccgggtca ccgcgccgca
      121 ctgcgtcgtc tacggcgaca aggagctgct cgtcaaggcg atccgcaacc tcgtcggcaa
      181 cgccatcgcc tactccgagt ccggcactca ggtcacggtg aaggtccgcc cctcgggcag
      241 cttcgtcgag gtgtccgtga gcgacgaagg ccaggggatc tcccccaccg accaggagcg
      301 gatcttcgaa cggttctacc gggtcgacgc ggcccggtcg cgggccaccg ggggcaccgg
      361 gctcggcctg gccatcgtca agcacgtctg cgtcaaccac ggcggagcgg tgagtgtctg
      421 gagccaggtc ggcgcgggct cgaccttcac gatccgcctt cccggggcga gcctgccccc
      481 gggcgagcct gccccgtcac gtcatacgca ccccatgacc gatcgctcgg cccactggta
      541 cggcggtccc gacccgtctt ccagcgccag gcccgccgac ccggccactg ctgtggacgc
      601 gaccccgggc accgcctcgc cgtttcgcga cccggcgccg gccgccggct cccgccgggt
      661 cccagtcggg gcccggacgc gcacggcggg cccccgcagt ccgcaacgcc actcccgaga
      721 ggccacccta tgagccgcat cctggtcgtc gaggacgaag agtccttctc cgaccccctg
      781 tcctacctcc tgcggaagga gggctacgag gtcgcggtcg ccgacacggg cccggcggcg
      841 ctcgccgagt tcgacaagca cggcgccgac ctcgtgctgc tcgacctcat gctcccaggc
      901 atctccggga ccgaggtatg ccgcaccctg cgccagcgct cggcggtgcc gatcatcatg
      961 ctcacggcca aggactccga gatcgacaag gtcgtcgggc tggagatcgg tgcggacgac
     1021 tacgtcacca agccctactc gtcccgggag ctgctcgccc ggatcaaggc cgtgctgcgt
     1081 cgcctcgccg agccggagga cccgctgccc gccacgatcg aggcggggcc ggtgcggatg
     1141 gacgtcgaac gccacatcgt cacggtcaag ggcgcgccgg tccccttgcc cctcaaggag
     1201 ttcgagctgc tcgagatgct cctgcgcaac tcgggccggg tcatgacccg ggtccagctc
     1261 atcgaccggg tgtggggcag cgactatgtg ggggacacca agaccctgga cgtgcacatc
     1321 aaacggctgc gggccaaggt cgagcccgac ccgggcaatc cgcggcacat cctcaccgtc
     1381 cgggggctcg gctacaagtt cgaggcctga cgtcgggctg cccgggctgc cggggtggtc
     1441 agctgcccgg ggtggtcagg gcgcgacaga ggccacggca gaggtcacgg cgccgcggcg
     1501 accggcgacg gcgtcaccgt cgagtagtag agcgtcggag gcaggaccgg gacctggacc
     1561 gtgacatctc cggaggtcgg cgtcgagacg accaggctca ccacggcgcc aggggcagcg
     1621 ccgatctggg ggatcgtcac cgaggtcagc ggagccgtgc ccccgggggg gaccatgacc
     1681 gacccgcccg gggccccggc cgagctgaac gccacccggg tcgccccgtc gccactgtt
//
LOCUS       DATAIX010000418         2937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_112643, whole genome shotgun sequence.
ACCESSION   DATAIX010000418 DATAIX010000000
VERSION     DATAIX010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112643"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1003)
                     /locus_tag="VHM65_06315"
     CDS             complement(107..1003)
                     /locus_tag="VHM65_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2705354.1"
                     /translation="MDTPISIRGLRKSFGATVALDGLDLEVATGEVHGFLGPNGSGKS
                     TTIRILLGLLRADGGTATLLGGDPWTDVESLHRRLAYVPGDVTLWPNLTGGEIIDLLG
                     RMRGGLDETRRAELVERFELDPTKRGRSYSKGNRQKVALVAALACDVELLLLDEPTSG
                     LDPLMDGVFRTYVDQLRKDGRTVLLSSHILSEVEALCDTISIIRAGRTVESGSLADLR
                     HLSRTSLDATLAVPMPAAAVGALPGVHGLTVDGARVRCSVETEHLGRVLGALTSHGVQ
                     SLISQPPTLEELFLGAYASPRP"
     gene            1279..2868
                     /locus_tag="VHM65_06320"
     CDS             1279..2868
                     /locus_tag="VHM65_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX2705355.1"
                     /translation="MADPVARTESDLPIQAMYDASALDGFDPATRLGAPGEYPFTRGV
                     YPSMYTGRPWTMRQYAGFGTAKESNERYHQLVAAGTGGLSVAFDLPTQMGYDSDDPVA
                     AGEVGKVGVAIDSLDDMRVLFDGLPLGKISTSMTINAPAATLLLLYQLVAQEQGVPGD
                     RLTGTVQNDVLKEYIARGTYIYPPKASLRLISDIFAYCAKEMPRWNTISISGYHMAEA
                     GATPAQEIAFTLANAKEYVRAAQAAGLDVDDFAPRLSFFFVVRTTLLEEVAKFRAARR
                     IWARIMREEFGATNPKSMMLRFHTQTAGVQLTAQQPEVNLVRVALQGLAAVLGGTQSL
                     HTNSFDEAIALPSPKAARLALRTQQVIAYETDVCKTVDPFAGSYVVEAMTDDLETAIV
                     ELIDAVEARGGAVAAIEQGFQKAEIERSAYRVALEIDSGERTIVGVNKFTIDSEENYD
                     PLRVDPVIEAEQRERLAALRSERDNDAVAAALAAMQEAARGTDNLLYPMRDALRLRAT
                     GGEVANALREVWGTYTPHDSF"
BASE COUNT          440 a         1037 c         1059 g          401 t
ORIGIN      
        1 gccgagctga tcgtcatccc cgcgagcgcg aggacgctga cggggatgag cacgcggtcg
       61 cgccgcaagg cgaggcggac gaggtgtccc gtgcccatgg tgccggtcat ggccgcgggc
      121 tcgcgtaggc gccgaggaac agctcctcca gcgtgggtgg ctgcgagatg agcgactgga
      181 cgccgtgcga ggtcaaggcg cccaggaccc ggccgaggtg ctcggtctcc acggagcacc
      241 gcacccgcgc gccgtcgacg gtcagcccgt gcacccccgg cagggcaccg accgctgcgg
      301 ccggcatggg gaccgccagg gtggcgtcga ggctggtgcg cgacaggtgc cgcaggtcgg
      361 cgagactgcc ggactcgaca gtccggcccg cccggatgat gctgatcgtg tcgcacaggg
      421 cctccacctc gctgaggatg tggctggaca gcagcaccgt ccggccgtcc ttgcggagct
      481 ggtcgacata ggtgcggaac accccgtcca tgagcgggtc cagccccgag gtcggctcgt
      541 cgagcaggag cagctcgaca tcgcaggcca gggcggcgac gagggcgacc ttctgccggt
      601 tgcccttgga gtacgagcgg cccctcttcg tcgggtcgag ctcgaagcgc tcgaccagct
      661 cggcgcggcg cgtctcgtcc aggcccccgc gcatccggcc gagcaggtcg atgatctcgc
      721 ccccggtgag gttgggccag agggtgacgt cgccgggcac gtaggccagg cgccggtgca
      781 ggctctcgac gtcggtccac gggtcgccac cgagcagggt ggcggtgccg ccgtcggccc
      841 ggagcaggcc gagcaggatg cggatcgtcg tggacttgcc ggacccgttc gggccgagga
      901 acccgtggac ctcgccggtg gcgacctcga ggtcgagccc gtcgagggcg accgtggcgc
      961 cgaagctctt gcgcagaccc ctgatcgaga tgggagtgtc catgcgtcga gcctacgacg
     1021 atccggccgg ccggcccgga gccatgccgg acccccgaag ggcgtgcggc cgggtccgcc
     1081 gaggtgggcc ggcaggtggt gcctgagtcc gctcacgatt ccgcggccga acaggcaggg
     1141 tgcctgccgc aggctccgcc ctcagtcgca gggtgaccca gcgcaaggcg gagcccgggg
     1201 gacgtcgggc gccagcgcca taccgttgtg acgttcgacc cctcgctgac cgggccgctc
     1261 gacagtaggc tcggctgcat ggctgaccct gtcgcacgca ccgagtccga cctgccgatc
     1321 caggcgatgt atgacgcgtc cgccctcgac ggcttcgacc cggcaacccg gctcggggcg
     1381 ccgggggagt accccttcac ccgcggcgtc tacccgtcga tgtacaccgg gcggccgtgg
     1441 acgatgcgcc agtatgccgg gttcggcacg gcgaaggagt ccaacgagcg ctaccaccag
     1501 ctcgtcgcgg cgggcaccgg cgggctgtcg gtcgccttcg acctgcccac gcagatgggc
     1561 tatgactcgg acgacccggt agccgccggc gaggtcggca aggtgggcgt cgcgatcgac
     1621 tcgctcgacg acatgcgggt gctcttcgac gggctgccgc tgggcaagat ctcgacgtcg
     1681 atgacgatca acgcccctgc cgcgacgctg ctgctgctct accagctcgt ggcgcaggag
     1741 cagggcgtcc ccggggaccg gctcaccggg acggtccaga acgacgtgct caaggagtac
     1801 atcgcccgcg ggacctacat ctacccgccg aaggcctcgc tgcggctcat ctccgacatc
     1861 ttcgcctact gcgccaagga gatgccgcgc tggaacacga tctcgatctc gggctaccac
     1921 atggccgagg cgggggcgac gccggcgcag gagatcgcgt tcaccctggc caacgccaag
     1981 gagtacgtgc gggcggccca ggccgccggg ctcgacgtcg acgacttcgc gccacggctc
     2041 tcgttcttct tcgtcgtgcg cacgacgctg ctcgaggagg tggcgaagtt ccgcgcggcg
     2101 cggcgcatct gggcgcggat catgcgcgag gagttcggtg ccacgaaccc caagtcgatg
     2161 atgttgcgct tccataccca gaccgcgggt gtgcagctga ccgcccagca gcccgaggtc
     2221 aacctcgtcc gggtcgcgct gcagggcctg gccgccgtcc tcggcgggac ccagtcactg
     2281 cacacgaact ccttcgacga ggcgatcgcc ctgccctccc cgaaggcggc tcggctcgcc
     2341 ctgcgcaccc agcaggtcat cgcctacgag accgacgtct gcaagacggt cgaccccttc
     2401 gccgggtcct acgtcgtcga ggccatgacc gacgacctcg agacggccat cgtcgagctc
     2461 atcgacgcgg tcgaggcgcg aggcggtgcg gtcgcggcga tcgagcaggg cttccagaag
     2521 gccgagatcg agcggtcggc ctaccgggtc gcgctcgaga tcgactccgg tgagcggacc
     2581 atcgtcgggg tcaacaagtt caccatcgac agcgaggaga actacgaccc gctgcgcgtc
     2641 gacccggtca tcgaggccga gcagcgcgag cgcctcgccg cgctgcgctc cgagcgcgac
     2701 aacgacgccg ttgccgcggc cctggccgcg atgcaagagg ccgcccgggg caccgacaac
     2761 ctgctctacc cgatgcgcga tgcgctgcgg ctgcgcgcaa cgggtggcga ggtggccaac
     2821 gcgctgcgcg aggtgtgggg cacctacacc ccccacgact ccttctgagg gcagcccgtg
     2881 gcccgttgga cgctgacgac gtccggcgcg cgcgcaggcg tcaggggagt gcccgga
//
LOCUS       DATAIX010000419         1912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_22760, whole genome shotgun sequence.
ACCESSION   DATAIX010000419 DATAIX010000000
VERSION     DATAIX010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22760"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VHM65_06325"
     CDS             <1..740
                     /locus_tag="VHM65_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2705356.1"
                     /translation="RTLRYYEELGLLAPARDAGGRRRYDGGTLDRLYRIRLLRELGTP
                     VADVNPDADDLRELTRRHLADLDARLAALARVRERVRAVQDRLLDGDRPGDEALLDLL
                     SGLPTDEPALTRRITLLVYRDIAAGHAYLVDVLGLAPGPISYDGDRAVHGEVHAGDGV
                     VWLHREAPEHRMVSPLTTGTVTASLAVLVDDVDAHHARVAATGAAIDYEPTDMPYGVR
                     EYSVRDPEGHLWSFQTPLTELDEQETP"
     gene            737..1126
                     /locus_tag="VHM65_06330"
     CDS             737..1126
                     /locus_tag="VHM65_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2705357.1"
                     /translation="MTQLTPYLCVADARAAIEWYVSALGAEVTYEPIVMDDGRVGHCE
                     LAIGEARWMMSDQFDSAGVAAPDPARGSAVSLHLTVTDVDAAAAAVVAAGTSMARGPE
                     DSPPAGRVAVFVDPFGHRWFLNQPAES"
BASE COUNT          262 a          710 c          667 g          273 t
ORIGIN      
        1 aacgcaccct gcgctactac gaggagctcg gcctcctggc tccggcccgc gacgccggcg
       61 gccgacgccg atacgacggc ggcaccctcg accggctcta ccgaatccgg ctgctccgag
      121 agctgggcac ccccgtggcc gacgtcaacc ccgatgcaga cgacctgcga gagctgaccc
      181 gccggcacct ggccgacctc gacgcccggc tggccgccct cgcccgggtg cgggaacggg
      241 tccgggccgt ccaggaccgg ctcctggacg gcgaccgccc cggggacgag gccctgctcg
      301 atctgctgtc gggcctgcca accgatgagc ccgcgttgac ccggcggatt acgttgctcg
      361 tgtaccgcga catcgcggcg gggcacgcct acctcgtcga cgtcctgggt cttgcccccg
      421 gaccgatcag ctacgacggc gaccgggctg tgcacggcga ggtccacgcc ggggacggcg
      481 tggtctggct gcaccgagag gcccccgagc accggatggt ctcgccgctg accaccggca
      541 cggtcaccgc ctcgctcgcc gtcctcgtcg acgacgtcga cgcccaccac gcccgggtgg
      601 ccgcgaccgg cgccgcgatc gactacgagc cgaccgacat gccctacggc gtccgcgagt
      661 acagcgtgcg cgaccccgag ggccacctct ggtcgttcca gacaccgctc accgagctcg
      721 atgaacagga gaccccatga cccagctgac tccctacctc tgcgtcgccg acgcccgagc
      781 cgccatcgag tggtacgtct ccgcgctggg cgccgaggtc acctacgagc cgatcgtcat
      841 ggacgacggc cgggtcggcc actgcgaact ggcgatcggc gaggcacgct ggatgatgtc
      901 cgaccagttc gactcggccg gggtggccgc ccccgacccg gcccgcgggt cggcggtgtc
      961 gctgcacctc acggtcaccg acgtcgacgc ggccgccgcc gccgtcgtcg cggccgggac
     1021 atcgatggcg cgcggccccg aggacagtcc gcccgccggg cgggtggcgg tcttcgtcga
     1081 ccccttcggt caccgctggt tcctgaacca gccggccgag agctgacaca gagccgctcg
     1141 gccaggcgag tcagtcccgg gctgtccagt gcgtcgagac ccacccgcca cccggcgacc
     1201 acgagtagaa ggcccaggcc gacggtcgcg ggtcagccac cgcgccgtag aacgatccgc
     1261 atgcgacgac ggtcagcgct gtcacccgtc ccgttgacga gacctgcgcg gacacctggc
     1321 cgccgcagct cgagcattcc cggcgcaccg gcgcaggttc cgcttgcgaa accgatgaca
     1381 tggaaggtga cgggggtgga tggcgttcgc agtctgcgcg ttggtcgtcg aggagggcgc
     1441 gaatcgcgcg gtacccgtgc gcggcctgcc ctcgagaagt gggcactcgt cattgttgcc
     1501 cgggggtcag cgtcggccgt ggcggccggt ggcgccaacc ctggccggcg gacgccggct
     1561 cgaccgcgac cccgaggccg accagcggcc tgtggcactg cgcgacgagg gatcggcgtg
     1621 cacctggggg cgcaggacgg gctgctgggt tagctcatcg cccgctggtt gctcctctgt
     1681 tcatcgctca tgacgtgccc ggtcgtgggt cggtcttcgc gtcgccggtg tcgggttcgt
     1741 aggggtagga gccgtgctgg tggatcatgc tcctcgtggt cgcggcgctg aaccacacgc
     1801 gtgccgcgcg accagctctc gatctgctcg ccgtcggcga gttcggcttc catgcggttg
     1861 agtccccacg cgacggcgag gtcaccgtcg atgacgagcc caatattggg cg
//
LOCUS       DATAIX010000420         1810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_22812, whole genome shotgun sequence.
ACCESSION   DATAIX010000420 DATAIX010000000
VERSION     DATAIX010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22812"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VHM65_06335"
     CDS             complement(<1..291)
                     /locus_tag="VHM65_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX2705358.1"
                     /translation="MTTDPHEERPLESSTMRIRGVGEVEHADAVLPAGLTAEDQATIE
                     ALRPGTAFLVVLRGPNTGARFLLDDTEITSGRHPDSDIFLDDVTVSRRHAVFT"
     gene            complement(429..818)
                     /gene="gcvH"
                     /locus_tag="VHM65_06340"
     CDS             complement(429..818)
                     /gene="gcvH"
                     /locus_tag="VHM65_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX2705359.1"
                     /translation="MSDLEYPDDLRYTTDHEWVAERDDGVVRVGITAFAQDALGDVVY
                     VQLPGVGDTLTAGDVSGEVESTKSVSDIYAPLTGEITAVNDTLDAAPEQVNSDPYGEG
                     WMYEMRPASASDVAGLLDVSAYKGTLG"
     gene            complement(880..1641)
                     /locus_tag="VHM65_06345"
     CDS             complement(880..1641)
                     /locus_tag="VHM65_06345"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEX2705360.1"
                     /translation="MSTDLPERSPWSRLRRMGAPRATRANVLATLLALLLGFGIATQV
                     QQTQQGGLEQLRQEDLVSLLDDISQRSARLDAQIRDLESDRETLRSGVGSSAAAVAQA
                     QARLDSLGILAGTLTAEGPGIRMTIEDSARKVTATTLLDVVEELRDAGAEVIQIGAAR
                     VVASTWLGDSGGDVQVDGTRVERPFMVLAIGDAATMTSAMNIPGGVVDTVRQLGASTS
                     IASVTTLAIDALHSAQTPRYAQAVPESVPSTPASR"
BASE COUNT          272 a          613 c          656 g          269 t
ORIGIN      
        1 ggtgaacacg gcgtgccggc gcgacacggt cacgtcgtcg aggaagatgt cgctgtccgg
       61 gtgacgtccc gaggtgatct cggtgtcgtc gaggaggaac cgggcacccg tgttgggtcc
      121 acgcagaacc acgaggaacg ccgtgccggg gcgcagcgcc tcgatcgtgg cctggtcctc
      181 ggcggtgagc ccggccggca ggacggcgtc ggcgtgctcg acctcgccga cgccgcggat
      241 ccgcatcgtg ctcgactcca gcggacgttc ctcgtgcggg tcggtggtca tcgaggtccc
      301 tcctccagag tggctcggcg ggcgcgtccg gcaatcgggc gtggcatcca ccctatcggc
      361 aagtgcgggg cagccggtat ggcgatcgcc cagctccctg cgcaggcacg ccgagccggc
      421 gcgggctgct agcccagagt gcccttgtag gccgacacat ccaggagccc ggcgacgtcg
      481 gacgcgcttg ccgggcgcat ctcgtacatc cacccctcgc cgtaggggtc cgagttcacc
      541 tgctcggggg cggcgtcgag cgtgtcgttg acggcggtga tctcgccggt caagggcgcg
      601 tagatgtcgc tcaccgactt ggtggactcg acctcaccgg agacgtcccc ggcggtgagc
      661 gtgtcgccga cgccgggcag ctggacgtag acgacatcac cgagggcgtc ctgggcgaag
      721 gccgtgatcc cgacccggac caccccgtca tcgcgctcag cgacccactc gtggtccgtg
      781 gtgtaccgca ggtcgtcggg gtactcgagg tcgctcatca gtctcctagc cgtgcacatc
      841 tcgtgggcgc ccgggcaggc ccttggtgga ctctgccgct caccgtgacg ccggcgtgct
      901 cggaacggac tccggcacgg cctgagcgta acgaggcgtc tgcgccgagt gcaacgcgtc
      961 gatggccagg gtggtcaccg aggcgatcga cgtggacgcc ccgagctggc gcacggtgtc
     1021 gaccacccct ccggggatgt tcatcgccga ggtcatcgtg gccgcatcgc cgatggcgag
     1081 caccatgaag ggtcgctcca ccctcgtccc gtcgacctgg acgtcccctc ctgagtcccc
     1141 gagccacgtc gaggccacga cccgggccgc gccgatctgg atcacctcgg cgccggcatc
     1201 acgcagctcc tcgacaacgt cgagcagcgt ggtcgcggtg accttgcgcg cgctgtcctc
     1261 gatcgtcata cggatgccag ggccttcggc ggtgagggtc cctgcgagga tcccgaggga
     1321 gtcgagccgg gcctgggcct gggcgaccgc cgcggccgag gacccgactc cgctgcgcag
     1381 cgtctcgcgg tcggactcga ggtcgcggat ctgtgcgtcg agccgagccg agcgctggga
     1441 tatgtcatcg agcagggaga ccaggtcctc ctggcgcagc tgttcgaggc ccccctgctg
     1501 agtctgctgg acctgggtcg caatgccgaa gcccaggaga agcgccagca aggtggcgag
     1561 gacgttggcg cgggtggccc ggggagcgcc catgcgccgc agccgcgacc acgggctgcg
     1621 ctcgggcagg tcggtactca tgcgcggaac aggtgccgac ggatcgaggc cacgttggag
     1681 aagatgcgca cgccgaggac gacgacgacg ccggtcgaga gctgagttcc gacgccaagc
     1741 tggtcgccga ggaagacgat gaacgcggcg acgacgacgt tggccaggaa ggacaccacg
     1801 aacactctgt
//
LOCUS       DATAIX010000421         6484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_129154, whole genome shotgun sequence.
ACCESSION   DATAIX010000421 DATAIX010000000
VERSION     DATAIX010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129154"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VHM65_06350"
     CDS             <1..487
                     /locus_tag="VHM65_06350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2705361.1"
                     /translation="TPLDPAAIARDAVAAAGPGYQAKGVCLDLNEPDAGPRVLADAER
                     IQQVLANLLDNALRHTPAGGQVSVSIHEGSDKTVGITVIDNGEGIPADQLAEVFERFY
                     RADPARAVHDGGGSGLGLTIARAIAEDHGGSLTASSAGPGSGSSFTLRLRPARTGPVR
                     G"
     gene            complement(477..2060)
                     /gene="serA"
                     /locus_tag="VHM65_06355"
     CDS             complement(477..2060)
                     /gene="serA"
                     /locus_tag="VHM65_06355"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX2705362.1"
                     /translation="MTKPVVLIAEKLSPATVAALGPDFEIRSCDGANRSELLAAIVDV
                     DAVLIRSATWIDAEALAAAKVLKVVARAGVGLDNVDVDAATAAGVMVVNAPTSNITSA
                     AELTVGLLLASARNIAPANAALKGGAWKRSAYGGVELLDKTVGIVGLGRIGSLVAERL
                     RGFGVRLLAYDPYTSAAKAGQLGARLVQLDELLAESDFITVHLPKTPETVGLIGAEAL
                     TKVKPTVRIVNAARGGIVDEVALAEAIREGRVAGAGLDVFATEPTTASPLFDFESVVV
                     TPHLGASTDEAQEKAGVSVARSVRLALAGDLVPDAVNVAGGAIDEHVRPGISLVERLG
                     RIFTAIAGAVPVQLDIDVKGEITEYDVSVWKLCALKGLFIDVVAEQVTYVNAPLLAEQ
                     RGCEVRLLTDATSGDFRNVTTLRGTLAHGTQVSVAGTLTGPKMVEKLVGVNGYDLEVP
                     LSDHLAIFEYADRPGIIGTVGRILGDNDINIGGMQVSRDDHRDHALVVLNVDSVLPQS
                     VAAQIADAIGAIQFSVVNL"
     gene            complement(2184..2537)
                     /locus_tag="VHM65_06360"
     CDS             complement(2184..2537)
                     /locus_tag="VHM65_06360"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX2705363.1"
                     /translation="MRVFDGLPFWLTFVVFFLGAAVRGSATYWAGRGLRSGAARTRWA
                     SRLDGVAVQRAEGVVARFGAPVVALSFLTVGVQTAVNAAAGAMRMPRRAPCPDLPPDR
                     PLLACRGPLRQPSAR"
     gene            complement(2589..3617)
                     /gene="ilvC"
                     /locus_tag="VHM65_06365"
     CDS             complement(2589..3617)
                     /gene="ilvC"
                     /locus_tag="VHM65_06365"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX2705364.1"
                     /translation="MAEMFYDDDADLSVVQGRKVAVIGYGSQGHAHALNLRDSGVDVR
                     VGLAEGSKSIAKAQDAGLTVRSVGDAVKDADLVVILTPDQVQRHVYAEHILPNLNPGT
                     TLLFGHGFNIRFGYIVPEAGSDVIMVAPKGPGHLVRREYVDGRGVPVLLAVEQDPSGH
                     AWDLVQSYAKAIGGLRAGGIRTTFTEECETDLFGEQSVLCGGVSQLVMYGFETLTEAG
                     YQPEVAYFECLHELKLIVDLMVEGGIAKQRWSVSDTAEYGDYVSGPRVLDPHVKDNMR
                     QILAEIKDGTFAARFIADQDAGAPEFKALRAQAEQHPIEATGRELRKLMSWIKTTDSD
                     YVEGTAAR"
     gene            complement(3730..4245)
                     /gene="ilvN"
                     /locus_tag="VHM65_06370"
     CDS             complement(3730..4245)
                     /gene="ilvN"
                     /locus_tag="VHM65_06370"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX2705365.1"
                     /translation="MSSKHTLSVLVENKPGVLARIAGLFSRRGFNIDSLAVGPTEHVD
                     ISRMTVVVQVDELPLEQVTKQLNKLIEVIKVVELDPTASVQRQVVLIKVRADAASRSH
                     ILEIVELFRANVIDVAADSLTIEAVGKSEKVNALLAVLEPYGVRELVQSGVVAVGRGP
                     RSITDRTLRSA"
     gene            complement(4251..6098)
                     /locus_tag="VHM65_06375"
     CDS             complement(4251..6098)
                     /locus_tag="VHM65_06375"
                     /inference="COORDINATES: protein motif:HMM:NF005860.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX2705366.1"
                     /translation="MSDVVAKPTPSDVAYQARRRPPGEPSGAPKVGPVECTGAQALVK
                     SLEAVGVDTVFGIPGGAILPAYDPLMDSKLVRHILVRHEQGAGHAAEGYAAASGRVGV
                     CMATSGPGATNLVTPIADAYMDSVPIVAITGQVPSASIGTDAFQEADIRGITMPITKH
                     NYLVTKAADIPRAIFEAFHIAGTGRPGPVLVDVAKDALQAKTTFSWPPQLDLPGYRPV
                     ARPHSKQIREAAKLIAAAKRPVYYVGGGVVRARASEELRHLVYLTQIPVVTTLMARGT
                     VPDRHPLNLGMPGMHGTVAAVTAMQQSDLLITLGARFDDRVTGLLSSFAPYAKVIHAD
                     IDPAEISKNRVADVPIVGDVKDVISDLIEVVTGDHATHGVPDYSEWRTQVKDWQDTYP
                     LGYTDPEDGLLAPQYVISRIGALTGPEATYVAGVGQHQMWAAQFIEYDRPNAWVNSGG
                     LGTMGFAIPAAMGAKTAEPERVVWAIDGDGCFQMTNQELATCVINNIPIKVAVINNSS
                     LGMVRQWQTLFYNQRYSNTDLHTSHDKVRIPDFVKLAEAYGCVGLRAEQPGEVDLVIE
                     QALAINNVPVLIDFVVHRDAMVWPMVPAGVSNDKIQIARHGAPIWERED"
BASE COUNT          972 a         2166 c         2337 g         1009 t
ORIGIN      
        1 caccccgctg gacccggccg ccatcgcgcg cgatgccgtg gccgccgcgg gccccgggta
       61 ccaggccaaa ggcgtgtgcc tggacctgaa cgaacccgac gccggtccgc gcgtgcttgc
      121 cgacgccgaa cggatccagc aggtcctggc caatctcctc gacaacgcgc tccggcacac
      181 gcccgccggc ggccaggtga gcgtctcgat ccatgaaggc tcggacaaga cggtgggcat
      241 caccgtcatc gacaacggcg aggggattcc tgcggaccag ctggccgagg tattcgaacg
      301 gttctaccgc gccgacccgg cgagggccgt ccacgacggc ggcggcagcg gactcggtct
      361 caccatcgcc cgggccatcg ccgaggacca tggaggctca ctcacggcgt ccagtgccgg
      421 acccggcagc ggcagttcct tcaccctgcg cctgcgaccg gccaggaccg ggccggtcag
      481 aggttgacga cggagaactg gatggcgccg atcgcgtcgg cgatctgggc ggcgacggac
      541 tggggcagga cggagtcgac gttgagaacc acgagggcgt ggtcacggtg gtcgtcgcgg
      601 gatacctgca tcccgccgat gttgatgtcg ttgtcaccga ggatccgccc gaccgtcccg
      661 atgatgcccg gccggtcggc atactcgaag atcgccaggt ggtccgagag cggcacctcg
      721 aggtcgtagc cgttgacccc gacgagcttc tcgaccatct tggggccggt gagcgtcccg
      781 gccaccgaca cctgggtgcc gtgggcgagc gtgccacgca gcgtcgtgac gttgcggaag
      841 tcgccgctcg tcgcgtccgt gagcagccgg acctcgcagc cgcgctgctc ggcgagcagc
      901 ggggcgttga cgtaggtcac ttgctcggcg acgacatcga tgaacaggcc cttgagcgcg
      961 cagagcttcc acacgctgac gtcatactcg gtgatctcgc ccttgacgtc gatgtcgagc
     1021 tggacgggca ccgcgccggc gatggcggtg aagatccgcc cgagccgttc gacgagcgag
     1081 atcccgggcc ggacatgctc gtcgatcgcg ccaccggcga cgttgaccgc atccggcacg
     1141 aggtcaccgg caagggcgag gcgcaccgac ctggcgaccg agaccccggc cttctcctgg
     1201 gcctcgtcgg tcgaggcgcc gagatggggt gtgacgacga cggactcgaa gtcgaagagc
     1261 ggggaggcgg tcgtcggctc cgtggcgaag acgtcgagcc ccgccccggc gacgcgccct
     1321 tcccggatcg cctcggccag cgccacctcg tcgacgatcc cgccgcgggc cgcgttgacg
     1381 atccggaccg tcggcttgac cttggtgagg gcctcggcac cgatgagccc gaccgtctcc
     1441 ggggttttgg gcaggtgcac cgtgatgaag tcggactcgg cgagcagctc gtcgagctgg
     1501 acgagccgcg cgcccagctg tccggccttg gccgcggacg tgtaagggtc gtaggccagc
     1561 aggcgcaccc cgaagccgcg cagccgctcg gcgacgagcg acccgatccg gccgaggccg
     1621 acgatgccga cggtcttgtc gaggagctcg acaccgccat aggcactgcg cttccaggcg
     1681 ccccccttga gggccgcgtt ggccggtgca atgttgcggg cgctggccag gagcagcccg
     1741 acggtgagct cggccgccga ggtgatgttc gaggtcggtg cgttgacgac catgacaccg
     1801 gcggcggtcg ccgcgtcgac gtcgacgttg tccaggccga caccggcccg ggcaacgacc
     1861 ttgagcacct tggccgcggc cagggcctcg gcgtcgatcc aggtcgccga gcggatgagc
     1921 accgcgtcga catcgacgat ggccgcgagc agctcggagc ggttggcgcc atcacacgac
     1981 cggatctcga agtccggccc cagtgcggcg accgttgcgg gcgagagctt ctcggcgatg
     2041 agcacgacgg gcttggtcac gtcggtcctt tggacggggc ggcggtgtca acgggtgggg
     2101 caccactctg ggccgccccg tagtttacga gccctacccg ggtcgcctcg gcgctgacca
     2161 gtatctggcc ggtgaccttg cgctcaccgt gcggacggct gcctcagcgg ccctcggcat
     2221 gccaggagcg gtcgatccgg cgggagatcc ggacaaggag cccgacgagg catccgcatc
     2281 gcaccggcgg ccgcgttgac cgccgtctgc acaccgaccg tgaggaagga cagcgccacc
     2341 accggcgcac cgaagcgcgc gaccactccc tcggcccgct gcaccgcgac gccgtcgagc
     2401 cggctcgccc agcgggtccg cgccgcaccg ctgcgcaggc cgcgcccagc ccagtaggtc
     2461 gcgctcccac gcaccgccgc gccgaggaag aacacgacga acgtgagcca gaagggcagg
     2521 ccgtcaaaca cgcgcacctg cccatgcctt ggcggtcatg ggtcgggcag ccgccgctgg
     2581 tatgccggtc agcgcgccgc cgtgccctcg acatagtccg agtcggtcgt cttgatccag
     2641 ctcatcagct tgcgcagctc gcggccggtc gcctcgatcg ggtgctgctc ggcctgggcg
     2701 cgcaaggcct tgaactcggg ggcgccggcg tcctggtcgg cgatgaaccg ggcggcaaag
     2761 gtgccgtcct tgatctcggc gaggatctgg cgcatgttgt ccttgacgtg cgggtccagg
     2821 acgcgcgggc cggacacgta gtcgccatac tcggcggtgt cggagaccga ccagcgctgc
     2881 ttggcgatgc cgccctcgac catgaggtcg acgatgagct tgagctcgtg caggcactcg
     2941 aagtaggcga cctcgggctg gtacccggcc tcggtcaggg tctcgaagcc gtacatgacg
     3001 agctgcgaga cgccgccgca cagcaccgac tgctcgccga agaggtcggt ctcgcactcc
     3061 tcggtgaagg tcgtcctgat cccgccggcg cgcaggccgc cgatggcctt cgcgtaggac
     3121 tgcaccaggt cccacgcgtg accgctgggg tcctgttcga cggcaaggag cacgggaacg
     3181 ccgcggccgt cgacatactc gcggcgcacg agatgccccg ggcccttggg agcgaccatg
     3241 atgacgtcag acccggcctc gggcacgatg tagccgaacc ggatgttgaa gccatgcccg
     3301 aagagcagag tcgtgcccgg gttgaggttg ggcaggatgt gttcggcgta gacgtggcgc
     3361 tgcacctggt cgggggtcag gatcacgacg aggtcggcat ccttgacggc gtcaccgacc
     3421 gacctcacgg tcagacccgc gtcctgtgcc ttggcgatgc tcttgctgcc ctcggcgagt
     3481 ccgacccgga cgtcgacgcc ggagtcacgc aggttgagcg cgtgggcgtg gccctggctc
     3541 ccgtagccga tgaccgcgac cttgcggccc tgcacgaccg agaggtcggc gtcgtcgtcg
     3601 tagaacatct cggccatggt ggcgagctcc ttttggtggt gggttcaggg agggtatgcc
     3661 ggtcgcggtg aggcacgggt gactcggcgc agcggcggcg aggacggccc accgggccgg
     3721 gaccacggct caggcgctgc gcaacgtgcg gtcggtgatc gagcgcggac cacggccgac
     3781 cgccacgaca ccggactgga cgagctcacg cacgccatac ggctcgagca cggcgagcag
     3841 cgcgttgacc ttctcggact tgccgacggc ctcgatcgtc agcgagtcgg cggcgacgtc
     3901 gatgacgttg gcgcggaaca gctcgacgat ctcgaggatg tgcgagcgtg aggccgcgtc
     3961 ggcgcggacc ttgatgagga cgacctggcg ctggaccgag gcggtcgggt cgagctcgac
     4021 gaccttgatc acctcgatga gcttgttgag ctgcttggtc acctgctcga gcggcagctc
     4081 gtcgacctgg acgacgacgg tcatccgcga gatgtcgacg tgttcagtcg ggccgacggc
     4141 gagggagtcg atgttgaacc cccggcggct gaacaggccg gcgatccggg ccagcacgcc
     4201 cggcttgttc tccaccagca cggacagggt gtgcttgctg ctcatctcgg tcagtcctcc
     4261 cgctcccaga tcggggcacc gtggcgcgcg atctggatct tgtcgttgct gacccccgcg
     4321 gggaccatgg gccacaccat cgcgtcgcgg tggacgacga agtcgatcag gacgggcacg
     4381 ttgttgatgg cgagcgcctg ctcgatcacc aggtcgacct cgccgggctg ctcggcccgc
     4441 agcccgacgc agccgtaggc ctcggcgagc ttgacgaagt cggggatgcg gaccttgtcg
     4501 tggctcgtgt gcaggtcggt gttggagtag cgctggttgt agaagagcgt ctgccactgg
     4561 cggaccatgc ccaggctgct gttgttgatg acggcgacct tgatcgggat gttgttgatg
     4621 acgcaggtcg cgagctcctg gttggtcatc tggaagcagc cgtcgccgtc gatggcccac
     4681 acgacccgct cgggctcggc cgtcttggcc cccatggccg cggggatcgc gaatcccatc
     4741 gtgccgaggc caccggagtt gacccaggcg ttgggccggt cgtactcgat gaactgggcg
     4801 gcccacatct ggtgctgacc cacgcccgcg acatacgtcg cctcgggtcc ggtgagcgca
     4861 ccgatgcgag agatgacgta ctgcggggcg agcaggccgt cctcgggatc ggtgtagccc
     4921 agcgggtagg tgtcctgcca gtccttgacc tgcgtgcgcc actcgctgta gtccggcacc
     4981 ccatgcgtcg cgtggtcgcc ggtcacgacc tcgatgaggt cgctgatgac gtccttgacg
     5041 tctccgacga tgggcacgtc ggcgacgcga ttcttggata tctccgccgg gtcgatgtcg
     5101 gcgtggatga ccttggcgta gggcgcgaac gaggacagca gcccggtgac ccggtcgtcg
     5161 aaccgggcgc cgagggtgat gagcaggtcg gactgctgca tcgcggtcac cgcggcgacc
     5221 gtcccgtgca tgcccggcat accgaggttg agcgggtgac ggtcggggac cgtgccgcgg
     5281 gccatgagcg tggtcacgac cgggatctgg gtcaggtaga cgagatggcg tagctcctcg
     5341 ctcgcccggg cccgcacgac cccgccgccg acgtagtaga ccgggcgctt ggccgccgcg
     5401 atgagcttgg ccgcctcgcg aatctgcttg ctgtggggac gtgcgaccgg ccggtagccg
     5461 ggcaggtcga gctgcggcgg ccaggagaac gtcgtcttgg cctgcagggc gtccttggcc
     5521 acgtcgacga ggacgggtcc agggcggccg gtgccggcga tgtggaacgc ctcgaagatc
     5581 gcccggggaa tgtcggcggc cttggtgacg aggtagttgt gcttggtgat cggcatcgtg
     5641 atgccacgga tgtcggcctc ctggaaggca tcggtgccga tcgacgccga gggcacctgc
     5701 cccgtgatgg ccacgatcgg gacggagtcc atgtaggcgt cggcgatcgg ggtgacgagg
     5761 ttcgtcgccc ccggacctga ggtcgccata cagacaccga cccgcccgct tgcggcggcg
     5821 tagccctcgg ccgcgtgtcc ggcaccctgc tcgtgccgca cgaggatgtg ccggaccagc
     5881 ttcgagtcca tgagcgggtc gtaggccggc aggatcgccc cgccggggat gccgaagacg
     5941 gtgtcgacgc cgacggcttc gagggacttg acgagcgcct gcgcgcccgt gcactcgacg
     6001 ggcccgacct tgggcgcccc gctgggctcc cccggcggcc tgcgccgggc ctggtaggcc
     6061 acatctgagg gcgttggctt ggccaccacg tcgctcacgt caggtcactc atcctcggtt
     6121 ggtgtcatcg gtggttggtg tcatcggtct ggtcctattc tcgcccaaca aaaaaccccc
     6181 tggtcccggg tcgggacggc aagggggagg cgcgctgacg tcgctgcgtc agtcgcgccc
     6241 aggaactacg agaagtcgag ccatgtggca acagtgtcgc cccgcggtgg gacgtgtcaa
     6301 gcaccgggcg cgatggtctc accatccggc cgaaaccaat ggcggcgtcc cgggtgaggc
     6361 cgccacgata ggcccatgca gctgcccgtt cccctcaccc gttgagtgag cgcgggttgt
     6421 cgcttccggg gccgtgaagc gacaacccac gctcactcaa cgggtgggtc tgggggtgct
     6481 gacc
//
LOCUS       DATAIX010000422         2819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_22866, whole genome shotgun sequence.
ACCESSION   DATAIX010000422 DATAIX010000000
VERSION     DATAIX010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22866"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VHM65_06380"
     CDS             <1..916
                     /locus_tag="VHM65_06380"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX2705367.1"
                     /translation="IQCLTLLAVAPLLVGVMRQVRARLEGRVGPGAGQPWRDLVKLAG
                     KEPLQAPGTRFAAVMPVVMMGTSLLLVALLPLLATTTLPLVPDDLFVVVSVLLLGSVA
                     IAILGLDSGTAFGGLGSSRHMTIAALVEPTVLVAVYALSIPVGTSALSAIVAARIAEP
                     ATVASPVSLLALVALVLVVIAETGRLPVDNPSTHLELTMVHEAMILESSGRDLGWVEL
                     ASWLRLAALLGLVVNLAAPWGIAAEVTPRALGIALAALAVKLTLAGAAVAAVEVFLAK
                     LRLFRVPELLAGSFVLAFLAVTASYLVA"
     gene            925..1584
                     /locus_tag="VHM65_06385"
     CDS             925..1584
                     /locus_tag="VHM65_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705368.1"
                     /translation="MTDSLLAQSVALSTGVLLLTTVLQLWRRSFTGAIGLLAVQGAAL
                     AGLVATLGLAQAEPELGVVAVLVLAIKAGVIPLVLARTAAVTGVPREAPPRLNPTAGL
                     LLAALLTTVAYLASRSIIGETSSPTTRAVPVGLAMVLIGFLILLTRRQAMSQLIGFVV
                     LDNGIAAVAFLTTGGVPLIVELGVSLDVLLVVLILRVLAGRMLRAFGATDLDDLMELR
                     D"
     gene            1584..>2819
                     /locus_tag="VHM65_06390"
     CDS             1584..>2819
                     /locus_tag="VHM65_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX2705369.1"
                     /translation="MDVALLWTPLVVPTVLAAAALPRRGAAWSRYTPAVASLVTLVCG
                     LALVAVVRSDGPLEAAAGLLRADALSAYLVAVVGAVALTATWAGLTPRAEANPDGWFA
                     ALLCLFLGAMTLALLADNVGILWVAVEATTIATAFLVGHRGGRHALEAAWKYVVLGSV
                     GVAIAFLGVVLLYAATTAGGSPTLSWVRLLGSASTLDPNLVRIGAALAALGFATKAGL
                     APMHSWLPDAHSQAPAAVSGLMSGVLLSVAFYAILRVKTITDAVLGPDLMRGMLVAAG
                     LLSLLVAAALMLTQRDYKRLLAYSSIEHMGILALGAAVGGPIATGAVLVHILGHGLAK
                     AAMFVVAGRILSAFGSSRITDVRSLLARHPRLAVPFLAGMTALLGFPPFGLFFTEVAL
                     VLAGIQRGLGWAMAVTVLLL"
BASE COUNT          271 a         1099 c          934 g          515 t
ORIGIN      
        1 catccagtgc ctgaccctgc tcgccgtggc tccgctgctc gtcggcgtca tgcgccaggt
       61 gcgcgcccgc ctcgaaggcc gcgtcgggcc aggcgccggc cagccctggc gtgacctggt
      121 caagctcgcc ggcaaggagc ccctgcaggc gccgggcacc cgatttgctg cggtcatgcc
      181 cgtcgtcatg atgggcacga gcctgctcct cgtcgcactc ctgccgctgc tcgccacgac
      241 gacactcccg ctcgtgcccg acgacctgtt cgtcgtcgtc tcggtgctgc tcctcgggtc
      301 ggtcgcgatc gccatcctcg gcctggactc cgggacggcg ttcgggggcc tggggtccag
      361 ccgccacatg acgatcgccg ccctcgtcga gccgaccgtc ctcgtcgccg tctacgcgtt
      421 gtcgatcccc gtcggcacgt cggccctgtc cgctatcgtc gccgcgcgga tcgccgagcc
      481 ggccaccgtc gcctcgcccg tcagcctgct cgccctcgtc gccctcgtcc tcgtcgtcat
      541 tgccgagacc ggacggctgc ccgtcgacaa cccctcgacg cacctcgagc tgacgatggt
      601 ccacgaggcg atgatcctcg agagctcggg ccgtgacctc ggctgggtcg agctcgcgtc
      661 gtggctgcgc ctcgccgcgc tgctcgggct cgtcgtcaac ctcgccgcgc catgggggat
      721 cgctgccgag gtcaccccgc gcgcgctcgg catcgccctg gccgcgctgg cggtcaagct
      781 gaccctggcg ggtgcggccg tcgcggccgt cgaggtcttc ctcgccaagc tgcgcctctt
      841 ccgcgtcccc gagctgctcg ccgggtcctt cgtcctcgcg ttcctcgcgg tgaccgcgtc
      901 ctacctggtg gcgtgagtga cccaatgacc gactccctcc tcgcccagtc cgtggctctg
      961 tccaccgggg tcctgctcct cacgaccgtc ctgcagctct ggcggcgctc gttcaccggc
     1021 gcgatcggac tgctcgccgt ccagggggct gccctcgccg ggctcgtcgc aaccctcggt
     1081 ctcgcgcaag ccgagcccga gctgggagtc gtggcggtcc tcgtgctcgc catcaaggcc
     1141 ggcgtgatcc ctctcgtcct cgcgcgaacg gccgccgtga ccggggtccc ccgtgaggct
     1201 ccccctcggc tcaacccgac cgcagggctc ctgctggccg ccctgctcac gaccgtcgcc
     1261 tatctcgcga gccgctcgat catcggcgag acctcctcgc ccaccacccg ggccgttccg
     1321 gtcgggctgg ccatggtcct catcggcttc ctcatcctgc tcacccggcg tcaggcgatg
     1381 tcccagctca tcgggttcgt cgtcctcgac aacgggattg ccgctgtcgc gttcctcacc
     1441 actggcgggg tgccgttgat cgtcgagctc ggcgtctcgc tcgacgtgtt gctcgtcgtc
     1501 ctcatcctgc gggtcctcgc cggacgcatg ctgcgtgcct tcggcgccac cgacctcgac
     1561 gacctgatgg agctgcgcga ctgatggacg tcgccctgct ctggaccccg ctcgtcgtgc
     1621 cgacggtgct ggccgccgcg gccctgccga gacgcggcgc cgcctggagc cgctacacgc
     1681 ctgccgttgc ctcgctggtc acgctcgtgt gtggactcgc gctcgtcgcg gtcgtccgct
     1741 cggatggccc actcgaggcg gcggccggcc tcttgcgggc cgacgcgctc tcggcatacc
     1801 tcgtcgccgt cgtcggtgcg gtggcactca ccgccacctg ggccggcctg accccccgcg
     1861 cggaggcgaa ccccgacggc tggttcgccg cgctgctctg cctcttcctc ggggcgatga
     1921 cgctcgcgct gctcgccgac aacgtcggga tcctgtgggt cgccgtcgag gccacgacga
     1981 tcgcgacggc gttcctcgtc ggccaccgtg gcggccgcca cgctctcgaa gccgcctgga
     2041 agtacgtcgt gctcgggtcg gtcggggtcg cgatcgcctt cctcggggtc gttctcctgt
     2101 atgccgcgac aaccgcgggc gggtcgccca ccttgtcgtg ggtcaggctg ctcgggagcg
     2161 cctcgacgct cgacccgaac ctcgtgcgga tcggcgccgc cctggctgcg ctcgggttcg
     2221 cgaccaaggc cgggctcgcg ccgatgcaca gctggttgcc cgacgcgcac agccaggcgc
     2281 ccgcagcggt gtcggggctg atgtcggggg tgctcctgtc cgtcgcgttc tacgcgatcc
     2341 tgcgggtcaa gacgatcacc gacgccgtgc tcggtccgga cctcatgcgg gggatgctcg
     2401 tggcggctgg gctcctgtcg ctgctcgtcg cggctgcgct catgctgacg cagcgcgact
     2461 acaagcgcct cttggcgtac tcgagcatcg agcacatggg catcctggcg ctcggcgcgg
     2521 ccgtcggcgg gccgatcgcc acgggggcgg tcctggtcca catcctcggt cacgggctcg
     2581 ccaaggccgc catgttcgtc gtggccgggc gaatcctgtc ggccttcggc tcgtcccgga
     2641 tcaccgacgt gcgctcgctg ctggctcgcc accctcggct ggccgtcccg ttcctcgccg
     2701 gtatgacggc cctgctcggc tttccgccgt tcgggctgtt cttcaccgag gtcgcgctgg
     2761 tgctcgccgg gatccagcgc gggctcggct gggcgatggc ggtcaccgtg ctgctgctg
//
LOCUS       DATAIX010000423         3010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_112845, whole genome shotgun sequence.
ACCESSION   DATAIX010000423 DATAIX010000000
VERSION     DATAIX010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112845"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1983)
                     /locus_tag="VHM65_06395"
     CDS             complement(<1..1983)
                     /locus_tag="VHM65_06395"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2705370.1"
                     /translation="MYVKTLTMRGFKSFASATTLRLEPGITCIVGPNGSGKSNVVDAL
                     AWVMGEQGAKSLRGGKMEDVIFAGTSGRPPLGRAEVALTIDNTDGALPIDYTEVTISR
                     TMFRNGGSDYAINGTGCRLLDIQELLSDSGIGREMHVIVGQGQLDAILRATPEERRGL
                     IEEAAGVLKHRKRKERALRKLETMDVSLTRLHDLTTEIRRQLGPLGRQAETARRAAVI
                     QAEARDARARLLADDLTSLTGTLEQEVADESALVARRAEVEAAIAGHSGRLGDLEAQA
                     EAGRPLLARAQERWFTLTQLSERLRATGTLAAERVRLLGEDDESESALASARDPEALR
                     AQAAELRGSTRRLAGEIAEAATALTEASVARAEAERQYAAEQARLSDFGRAAADRREG
                     LATLAGQVAAKASRIESGDAEIGRLREIGAQSLARAGEAEREFAALESSITDDEDGEQ
                     GLDSAYESAAARLAEAEARIERLREQERAAERDRSSGSARLEALELSLRRKDGATALL
                     AAADAGVLGSVVGLVEVRDGHEAGVAAALGWAADALAVDSLDVAGRALDLLRTQDAGQ
                     VGLLVAESATALDRSGWPQLPPGAVWAADVLSAPASLRPALDAVLARVALVPDSGMAQ
                     LLVRAQVTAVTADGDVYAPGWVRGGSHGAPSLIEIHA"
     gene            complement(2132..2473)
                     /locus_tag="VHM65_06400"
     CDS             complement(2132..2473)
                     /locus_tag="VHM65_06400"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF006691.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX2705371.1"
                     /translation="MSTEPEQGAASTADRAPADEMPPELARLRRSIDNIDAALVHLLA
                     ERFSCTQAVGRLKAERGMPPADPAREERQIARLRVLADEAGLDPVFAEKFLTFVIAEV
                     IRHHRALAAGG"
     gene            complement(2582..>3010)
                     /locus_tag="VHM65_06405"
     CDS             complement(2582..>3010)
                     /locus_tag="VHM65_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX2705372.1"
                     /translation="ELVARMRDADHFGKGYQARTQMFYAAMSYYFHVEQPEDITARCR
                     ELQAAYSLFPYIEGTHMPAGFGHLEGYGSAYYTYMWSLVIAKDMFSAFDPADLFDPEV
                     AGRYRDRVLAPGGQRDAADLVADFIGRPYTVAAYERWLAQ"
BASE COUNT          380 a         1099 c         1124 g          407 t
ORIGIN      
        1 ggcgtggatc tcgatgaggc tcggcgcgcc gtggctgccg ccccggaccc accccggtgc
       61 gtagacgtca ccgtccgccg tgaccgcggt gacctgcgca cggacgagga gctgcgccat
      121 accgctgtcc gggacgagcg cgacccgcgc caggacggca tcgagggccg gacgcagtga
      181 cgcaggggca ctgagcacgt cggcagccca caccgccccc gggggcagct gcggccagcc
      241 ggaccgatcc agcgccgtcg cagactctgc gacgagcagg cccacctggc cggcgtcctg
      301 ggtccgcagc aggtcgagcg cccgcccggc gacgtccagc gagtcgaccg cgagcgcatc
      361 ggcggcccag ccgagggccg cggcgacccc ggcttcgtgg ccgtcgcgga cctcgacgag
      421 cccgacgacc gagccgagca ccccggcatc ggcagcggcg agcagtgctg tggcgccgtc
      481 cttgcggcgc aggctcagct cgagcgcctc gaggcgggcc gagcccgacg aacggtctcg
      541 ctcggcggcg cgctcctgct cgcgcagccg ctcgatccgg gcctcggcct cggccagccg
      601 cgccgccgcg ctctcatagg ccgagtcgag cccctgttcg ccgtcctcgt cgtcggtgat
      661 cgaggactcc agcgccgcga actcccgctc ggcctcgccg gcccgggcca gggactgcgc
      721 gccgatctcg cgcagccgcc cgatctcggc atcgcccgac tcgatccgcg acgccttggc
      781 ggcgacctgc ccggccagtg tcgccagtcc ctcgcgccga tctgccgcag cccgcccgaa
      841 gtcgctcagc cgggcctgtt cagcggcata ctgccgctct gcctcggcgc gggcgacgct
      901 ggcctcggtg agggcggtgg ccgcctcggc gatctccccc gcaagccggc gggtcgagcc
      961 ccgcagctcg gcggcctggg cgcgcagggc ctcggggtcc cgggccgagg ccagggcgga
     1021 ttcggactcg tcgtcctcac cgagcaggcg aacccgctcg gcggcgaggg tgcccgtggc
     1081 ccggagtcgc tcgctgagct gggtcagggt gaaccagcgc tcctgggcgc gggccagcag
     1141 cgggcgaccc gcctcggcct gagcctcgag gtcgccgaga cggccgctgt gcccggcgat
     1201 ggcggcctcg acctcggcac gccgtgccac cagggcgctc tcgtcggcga cctcctgctc
     1261 gagggtgccg gtgagcgagg tcaggtcatc ggcgagaagc cgcgcccggg catcgcgggc
     1321 ctcggcctgg atcaccgccg cgcggcgggc ggtctcggcc tgccgaccca gggggccgag
     1381 ctggcggcgg atctcggtcg tgaggtcgtg cagccgggtc aggctcacgt ccatcgtctc
     1441 gagcttgcgc agagcgcgtt ccttgcgctt gcggtgcttg aggaccccgg ccgcctcctc
     1501 gatgagcccc cggcgctcct cgggtgtggc ccgcaggatc gcgtcgagct ggccctggcc
     1561 gacgatgacg tgcatctccc ggccgatgcc cgagtccgac aacagctcct ggatgtcgag
     1621 caggcgacac ccggtgccgt tgatcgcgta gtccgagccg ccgttgcgga acatcgtccg
     1681 cgagatggtc acctcggtgt agtcgatcgg gagggcaccg tcggtgttgt cgatggtcag
     1741 tgccacctcg gcccggccca gcggggggcg gcccgaggtc ccggcgaaga tgacgtcctc
     1801 catcttgccg ccgcgcaggg acttggcgcc ctgctccccc atgacccacg cgagcgcgtc
     1861 gacgacattg gacttgcccg agccgttggg gccgacgatg cacgtgatgc ccggttccag
     1921 acgcagcgtc gtcgcggacg cgaacgactt gaagccgcgc atggtgaggg tcttgacgta
     1981 cacgggctgc ggttctcctg gtgcggtcgt cgggacccgc tcaccctacc cgcgccatag
     2041 cgcgagtccg cggacccgcc ataccctcgt gcgggtcgcg gcacaggtga ggcacccgtg
     2101 gcgcgggcac gacgcgccgg cccgggctcg gtcagccgcc cgcggcgagg gcccgatggt
     2161 ggcggatcac ctcggcgatg acgaaggtga ggaacttctc ggcgaagacc gggtcgagcc
     2221 cggcctcgtc ggccaggacg cgcagccggg cgatctggcg ctcctcgcgg gccgggtcgg
     2281 ccggcggcat gccacgctcg gccttgagcc ggccgaccgc ctgggtgcag ctgaaccgct
     2341 cggcgaggag gtggacgagg gccgcgtcga tgttgtcgat gctccggcgc agccgcgcca
     2401 gctctggcgg catctcgtct gcgggcgcgc ggtcggccgt ggacgcggcg ccctgctcag
     2461 gctcagtgct cacgagccac agcgtaggcg gcgtcggcca ctcggcgcgc caggtgcggc
     2521 cccgtggacg gcgctgcagc gaaacttcgg gcgacgtgcg gcacgcacag gacgccggcg
     2581 gctactgcgc cagccagcgc tcgtaggccg cgaccgtgta gggccgcccg atgaagtccg
     2641 cgacgaggtc ggccgcgtcg cgctgcccgc caggtgcgag cacgcggtcg cggtagcgcc
     2701 cggccacctc ggggtcgaac aggtcggccg ggtcgaaggc gctgaacatg tccttggcga
     2761 tgacgaggga ccacatgtag gtgtagtacg ccgacccgta gccctcgagg tgcccgaagc
     2821 cggccggcat gtgggtgccc tcgatgtagg ggaagaggga gtatgccgcc tggagctcgc
     2881 ggcatcgggc cgtgatgtcc tcgggctgct cgacgtggaa gtagtaggac atcgcggcgt
     2941 agaacatctg ggtgcgcgcc tggtagccct tgccgaagtg gtcggcgtcg cgcatccggg
     3001 cgaccagctc
//
LOCUS       DATAIX010000424         7034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_112848, whole genome shotgun sequence.
ACCESSION   DATAIX010000424 DATAIX010000000
VERSION     DATAIX010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 7034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7034
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112848"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..1858
                     /locus_tag="VHM65_06410"
     CDS             266..1858
                     /locus_tag="VHM65_06410"
                     /EC_number="1.2.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF006916.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
                     /protein_id="HEX2705373.1"
                     /translation="MASETLVDPELDPSATFAVDPALVRRLTARVVCGPRPERVVTHS
                     PLTGAPVANLPRSTPADVAVAYETAQAAQRRWCRTPVSARAAVLLRLHDIVLARQPEI
                     LDLIQLESGKARVHAFEEVADVAIASRHYGRVGPRLLRPHRRAGLAPLLTQTVESHLP
                     IGVVGIVSPWNYPFSLAVTDALPALLAGNAVLLRPDVLTSLCALMGAELLAEAGLPDG
                     VLQIVLGAGSTVGQAVLDRADYVCYTGSTAVGRSVAQRAAMRLVGASLELGGKNSLYV
                     RADADLDRAVLGITRSAFSSAGQLCMHTERVVVDASVADELLAKLVPAVEAMRLGAEL
                     AFGPDMGSLMSKSQLTRVTEHVEDARAKGARVLAGGRPRPELGPYFYEPTLLEGVTPA
                     MSCSGEETFGPVVAIYRVSGDDEALALMNDTEYGLHASIFSRDVRGARSLARRVRTGT
                     VSINEGYLVTWGSVAAPLGGMKQSGIGRRHGAEGLLRFTEPQNVSAQHGPMIGPIGSM
                     SQEQFAAGLTKALRAMKALGRP"
     gene            1873..3591
                     /locus_tag="VHM65_06415"
     CDS             1873..3591
                     /locus_tag="VHM65_06415"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX2705374.1"
                     /translation="MDTFTGTAPAATYDHDVIVIGSGFGGAVTALRLAEKGYRVHVLE
                     SGRRFEDADFAKTSWDLRRYLWAPRLGCYGVQRIHRLPDVLLLAGAGVGGGSLNYACT
                     LYVPPQPFFTDPQWSHITDWQAELEPHYATASRMLGVVTNPCNGPVEQLMKQTADDLG
                     MGDTFRHTPVGVFFGEPGRRVGDPYFGGTGPDRVGCTECGNCMVGCRIGAKNTLRKNY
                     LALAERLGVTIEPLRTVVHLRALDPGHPERGYAVTHERSGAWARKDSTTVTAEQVVVA
                     AGTWGSQKLLHAMAAAGRQSPQRGLPRLSARLGSLTRTNSESLNGASAVTVPPGVDLT
                     HGVAITTSFHVDEDTHVENVRYGRGSNAMGLLATIMVEGGGRTPRTARFLGDVARRPR
                     TFARSLSVRRWSERTVIALVMQSRDNSLSVTGRRGLLGGRALTSRQGHGEANPTWIPA
                     GQRAVHALAQGLARDTGVPANAGGTVTEIVNVPITAHFLGGCVIGDSPGHGVIDPYHR
                     VWSYPGLHVVDGSAISANLGVNPSLTITAQAERAMSLWPRRGEPDRRPAQGEPYVRLG
                     GATATV"
     gene            complement(3640..5415)
                     /locus_tag="VHM65_06420"
     CDS             complement(3640..5415)
                     /locus_tag="VHM65_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2705375.1"
                     /translation="MALSVEPSVRTLPVADRASVRAHTRDTLVRHGRSLAVVVGLHAL
                     AAVASLAAPRIIGQLVDMVSHGTTVEAIDHLAVILAVAVVAQTIFMWAARRASFILGE
                     TVFADLREEFMDRVVALPLSTVERAGTGDLVSRTTNDVEALSHVVRFGIPSIFVAAIT
                     LVIYTGAALVTGWAVALSVFVGVPLMWVSTRWYLRYAPDGYLRERATYAVMNGVVTES
                     VDGARTIESLRLATLRRTRMDDSLRACVKAELYTLRLRMLWFPCVEFAYVVPIAVTLV
                     WGGWLVSAGHTTVGVVTSVALYVQLLAEPLDELLSWLDEIQVGATSLARVIGIADVPP
                     DREATGGTPTGSSFEVRDASYAYRAGRDVLHGVSLDLHPGERLAIVGPSGAGKSTLGR
                     LLAGIDGPRDGRVSVGGVRLVDLTLDQLRGEVALVTQEHHVFVGTLEQNLLLARPGAT
                     RAELAAALAAVDAAEWVAALPSGLDTVVGSGGQVLTEGQSQQLALARLVLADPHTLVL
                     DEATSLLDPRAARHLERSLAAVVQGRTVVAIAHRLHTAHDADRVAVVEDGQITEVGSH
                     DELIAQRGAYAALWRSWRDEPAISP"
     gene            complement(5417..>7034)
                     /locus_tag="VHM65_06425"
     CDS             complement(5417..>7034)
                     /locus_tag="VHM65_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2705376.1"
                     /translation="VWMVSQALLPAAISRAIDDGLIGEDRGSLWLWAGAVFGLAVLVA
                     VSGSFRHMYAVRNWLGAAIRAAQLIGWKAADTGAALPRTVPTGEVVATVASDAMRLGG
                     VYDVVARFAGAVVSYVVVAVILLQASPPLGLLVLIGVPVMLGLLSFIVRPLQRRQARQ
                     REESGKLITLGADTVAGLRVLRGIGGEATFLARYAAQSQQVRRVGVRVAGVQAALDAS
                     HVLLPGIFVLLVTWFGARFALEGKISPGQLVAFYGYSAFLVQPIGTANEFVDRFTRAH
                     IASRKLIRVLGVAPDQAEVAAGTGAPVSSRVNGSHPAPLLVDPVSGVSVEPRLLTAVV
                     SARPEDSVAVADRLGRFGPGRGGALLSGVPVDDMPLSEVRARVLVSENDPRLFTGVLR
                     EELDPSGRHHEIDLLDALTVASGDDILQALPDGLDSLVEERGRSFSGGQRQRLALTRA
                     LLADAEILVLIEPTSAVDAHTEARVAARLAQARRGRTTVVMTASPLVLDHADRVLFLD
                     GGVVTAAGSHHELMRTCPPYRSTVTRGEGD"
BASE COUNT         1026 a         2514 c         2564 g          930 t
ORIGIN      
        1 gccgggcacg gtgctctcgc tctcgacccg gtaggtggcg ttctccgagt ggttgaccag
       61 agtcagcgcg gcctccgcgg gcaggccgaa agccgccagc gctgctcgcg cgtaggcgtc
      121 atacgcgctg gcaggggttc ggtcgctcgg tgcctgcatg gtcctcctgc gccgggcccg
      181 ggttcgtcag cacggtatcg gaacccgggt gcgagatggg tcccacccgc ccgccgagcc
      241 cggcctggtg acttaacctg ggcttatggc ctcagaaacc cttgttgacc ccgagctcga
      301 cccttccgcg acgtttgccg tcgacccggc gctcgtacgg cggctcaccg cacgcgtcgt
      361 gtgtgggccc cgaccggagc gggtcgtcac ccactccccg ctgaccggag ccccggtcgc
      421 gaacctcccg cggtcgactc cggctgacgt ggcggtggcg tacgagacgg cccaggcggc
      481 ccagcgccgc tggtgccgga cacccgtgtc cgcccgggcc gcggtcctgc tgcggctgca
      541 cgacatcgtg ctcgcacgcc agcccgagat cctcgacctc atccagctcg agtcgggcaa
      601 ggcccgggtg cacgcgttcg aggaggtcgc ggacgtcgcg attgcctccc ggcactatgg
      661 ccgggtcggt ccgcgcctgc tgcgcccgca ccgccgggcc ggtctggccc cgctgctgac
      721 ccagaccgtc gagtcccacc tgccgatcgg cgtcgtcggg atcgtctcac cctggaacta
      781 cccgttctcc ctcgccgtga ccgatgccct tccggccctg ctcgcgggca acgcggttct
      841 ccttcggccg gacgtgctga cctccctctg tgcgctcatg ggcgccgagc tgctcgccga
      901 ggcgggactg cccgatggtg tcctgcagat cgtcctggga gccggctcga ccgtggggca
      961 ggcggtgctc gaccgggccg actacgtgtg ctacacgggc tcgacggcgg tcgggcgctc
     1021 ggtggcccag cgcgcggcaa tgcgcctcgt tggcgcctcc ctcgagctcg gcggcaagaa
     1081 ctcgctctac gtccgggccg acgccgatct cgaccgggcc gtgctgggca tcaccaggtc
     1141 ggccttctcc tcggcggggc agctgtgcat gcataccgag cgggtcgtcg tggacgcgtc
     1201 ggtcgccgat gagctcctcg ccaagctcgt cccggcggtc gaggccatgc ggctcggcgc
     1261 cgagctcgcc ttcggccccg acatgggcag cctgatgtcg aagagccagc tcacccgggt
     1321 gaccgagcac gtcgaggacg cccgcgccaa gggcgcccgt gtcctggccg ggggccgccc
     1381 caggccagag ctcggcccgt acttctacga gccgaccctc ctcgagggag tgacccctgc
     1441 catgtcctgc agcggcgagg agaccttcgg gccggtcgtc gcgatctacc gggtcagcgg
     1501 cgacgacgag gccctcgctc tgatgaacga caccgagtac ggcctgcacg caagcatctt
     1561 cagccgcgat gtccgcggcg cccggtcgct cgcgcgccgg gtccgcaccg ggacggtgag
     1621 catcaacgag gggtatctcg tcacctgggg cagcgttgcg gctcccctcg gcgggatgaa
     1681 gcagtccggg atcggccggc gccacggcgc cgaggggctg ctgcgcttca ccgaaccgca
     1741 gaacgtctcg gcccagcacg gaccgatgat cggacccatc gggtcgatgt cgcaggagca
     1801 gttcgcggcg ggcctgacca aggccctgcg ggccatgaag gccctgggcc ggccatgagc
     1861 aaggagacac gtgtggacac cttcactgga acggcaccgg ccgcgacata cgaccacgat
     1921 gtgatcgtca tcgggtcggg cttcggcggc gcggtcacgg cgctgcgcct ggccgagaag
     1981 ggctaccggg ttcacgtcct cgagtcgggg cgccggttcg aggacgcgga ctttgccaag
     2041 acctcctggg acctgcggcg ctatctctgg gcgccccggc tcggctgcta cggcgtccag
     2101 cggatccaca gactgcccga cgtcctgctg ctggcgggag ccggggtggg gggtgggtca
     2161 ctgaactacg cctgcaccct ctacgtgccg ccacagccgt tcttcaccga cccgcagtgg
     2221 agccacatca ccgactggca ggccgagctc gagccccact acgccacggc gagccgcatg
     2281 ctcggcgtcg tcaccaaccc gtgcaacggg cccgtcgagc agctcatgaa gcagacggcc
     2341 gacgatctcg ggatgggaga cacgttccgc catactcccg tcggggtgtt cttcggtgag
     2401 ccggggcggc gcgtggggga cccctacttc ggcggtacgg ggccggaccg cgtcggttgc
     2461 accgagtgcg gcaactgcat ggtcgggtgc cgcatcggcg ccaagaacac cctgaggaag
     2521 aactacctgg cgctcgccga gcggctcggc gtcacgatcg agccgctgcg caccgtggtc
     2581 cacctgcgtg ccctcgaccc cggccacccc gagcgcgggt atgccgtcac gcacgaacgc
     2641 tccggggcct gggcccgcaa ggactccacg acggtcacgg ccgagcaggt cgtcgtcgcc
     2701 gcaggcacct ggggcagcca gaagctgctc cacgccatgg ctgctgcggg gcgccagagc
     2761 ccgcagcgtg gcctgccccg gctctcggcc cggctcgggt cgctgacccg gaccaactcg
     2821 gagtcgctca acggggcctc ggccgtcacc gttcccccgg gggtcgacct gacccacggc
     2881 gtggcgatca cgacatcgtt ccacgtcgac gaggacaccc atgtcgagaa tgtccggtac
     2941 ggacgtggat ccaacgccat ggggctcctt gcgacgatca tggtcgaggg cggggggcgg
     3001 acgccccgga cggcgcgctt cctcggtgac gtcgcccgcc gaccgcggac cttcgcgcgg
     3061 tcgctgtcgg tgcggcgctg gagtgagcgg accgtcatcg ccctcgtcat gcagtcgcgg
     3121 gacaactccc tgtccgtgac gggccgacgc ggcctgctcg gcgggcgggc gctgacctcc
     3181 cggcagggcc atggggaggc gaacccgacg tggatcccgg ccggccagcg agcggtgcac
     3241 gcgcttgccc aggggctggc tcgtgacacc ggcgtgccgg cgaacgctgg cggtacggtg
     3301 accgagatcg tcaacgtgcc catcacggcg cacttcctcg gcggctgcgt catcggtgac
     3361 agccctgggc acggggtgat cgacccctac caccgggtgt ggtcctaccc gggcctgcac
     3421 gtcgtcgacg gctcggccat atcggccaac ctcggggtca acccgtcgct gacgatcacc
     3481 gcccaggccg agcgggccat gtccctgtgg ccccgtcgcg gtgagcccga ccgccgcccc
     3541 gcccagggcg agccctacgt tcggttgggt ggcgccacgg ccacggtatg aggggccccc
     3601 atcgacctcg gtgggcacgt cctcgcaccc cgcgccctgt catggcgaga tggcgggttc
     3661 atccctccac gagcgccaca gggccgcgta ggcgcctcgc tgggcgatga gctcgtcgtg
     3721 cgatcccacc tcggtgatct gcccgtcctc gacgaccgcc acccggtcgg cgtcgtgcgc
     3781 ggtgtgcagc cggtgggcga tcgcgacgac cgtgcggccc tgcacgactg cggcgaggga
     3841 gcgttccagg tgccgcgccg cgcgggggtc cagcagggag gtggcctcgt cgagcacgag
     3901 ggtgtgtggg tcggcgagca cgagccgggc caacgcgagc tgctgggact ggccctcggt
     3961 gaggacctga ccacccgaac cgacgaccgt gtccagaccg gacggcagcg cggccaccca
     4021 ctcggcggca tccacggcgg cgagcgcagc ggcgagctcg gcccgcgtgg cgcccggccg
     4081 ggcgagcagg aggttctgct ccagggtgcc gacgaagacg tgatgctcct gcgtcaccag
     4141 ggcgacctcg ccgcggagct ggtcgagggt gaggtcgacc aggcgcaccc cgccgacaga
     4201 gacgcgcccg tcgcggggtc cgtcgatccc ggccaggagc cggcccaggg tcgacttgcc
     4261 cgcaccggag gggccgacga tggccaggcg ctcgccgggg tgcaggtcga gggagacccc
     4321 gtgcaggacg tcgcgcccgg cgcggtaggc gtacgacgcg tcgcggacct cgaacgagga
     4381 cccggtcggt gtcccgccgg ttgcctcacg gtcgggtggg acgtcggcga tcccgatgac
     4441 ccgggcgagt gaggtcgcgc cgacctggat ctcgtcgagc caggacagca gctcgtccag
     4501 gggctcggcg agcagctgca cgtagagggc gaccgaggtg acgaccccga cggtcgtatg
     4561 cccggccgac accagccagc cgccccagac aagcgtgacg gcaatgggca cgacatacgc
     4621 gaactcgaca cagggaaacc agagcatccg cagccgcagc gtgtagagct cggccttgac
     4681 gcacgcgcgc agggagtcgt ccatgcgggt ccgccgcagc gtggcgagcc gcagtgactc
     4741 gatcgtccgg gcgccgtcga cgctctcggt cacgacaccg ttcatcaccg cgtatgtcgc
     4801 gcgctcgcgc aggtagccgt ccggtgcgta gcgcaggtac cagcgggtcg agacccacat
     4861 gagcggcaca ccgacgaaga ccgacagcgc gaccgcccac ccggtgacca gggccgcgcc
     4921 ggtgtagatg acgagggtga tggccgcgac gaagatcgac gggatgccga agcgcacgac
     4981 gtggctcagg gcctcgacgt cgttcgtcgt ccgggacacg aggtccccgg tgcccgcgcg
     5041 ctcgaccgtc gacaacggca gcgcgacgac ccggtccatg aactcctcgc gcaggtcggc
     5101 gaagaccgtc tcgccgagga tgaacgaggc tcgccgggcg gcccacatga agatcgtctg
     5161 ggcgacgacg gcgaccgcga ggatgaccgc gaggtggtcg atggcctcga cggtggtgcc
     5221 gtgggagacc atgtcgacga gctggccgat gatccggggc gccgcgaggg aggcgaccgc
     5281 ggccagcgca tgcaggccga cgaccacggc gagactgcgc ccgtgccgca cgagggtgtc
     5341 gcgggtgtgg gcgcgcaccg acgcgcggtc ggcgacgggc agcgtgcgga cgctgggctc
     5401 gacgctcagt gccatctcag tcaccctccc cacgggtcac ggtcgatcgg tatggcggac
     5461 acgtgcgcat gagctcgtgg tgggacccgg cggcggtgac gacacccccg tcgaggaaga
     5521 gcacccggtc ggcgtggtcg aggacgaggg ggctcgcggt catgacgacg gtcgtccggc
     5581 cacggcgggc ctgcgcgagc cgagccgcga ccctggcctc ggtgtgggcg tcgacggcgc
     5641 tcgtcggctc gatgaggacg aggatctcgg cgtcggcgag cagggcccgg gtcagtgcga
     5701 gccgctggcg ctgccctccc gagaacgacc ggccgcgctc ctcgaccagg ctgtcgagcc
     5761 cgtccggcag ggcctgcagg atgtcgtcgc cgctcgcgac ggtgagcgcg tcgagcaggt
     5821 cgatctcgtg atggcgcccg gacgggtcga gctcctcgcg gagtacgccg gtgaacaggc
     5881 gtgggtcgtt ctcgctgacg agcacgcgcg cgcggacctc cgacaggggc atgtcgtcga
     5941 ccggcacccc cgagaggagt gcgccaccgc gacctggccc gaacctgccc aggcggtcgg
     6001 ccacggcgac cgagtcctcg ggccgagccg acacgacggc cgtgagcagc ctgggctcga
     6061 cgctgacccc gctgacgggg tcgacgagca gcggagcggg atgtgagcca ttgacccgtg
     6121 acgagacggg cgcaccggtc ccggccgcga cctcggcctg gtccggggcg acgccgagca
     6181 cccggatcag cttgcgggag gcgatgtgag cccgggtgaa ccggtcgacg aactcgttgg
     6241 ccgtgccgat cggctggacg aggaaggccg agtagccgta gaaggccacg agctggcctg
     6301 gcgagatctt gccttccagc gcgaaccgcg ccccgaacca cgtcacgagc agcacgaaga
     6361 tgccgggcag caggacatgg gacgcgtcga gcgcggcctg gacgcccgcc acgcgcaccc
     6421 cgacccggcg cacctgctgg gactgggcgg cataccgggc gaggaaggtc gcctcgccac
     6481 cgatcccgcg gagcacccgc aggcccgcga ccgtgtcggc gccgagggtg atgagcttgc
     6541 cggactcctc acgctggcgt gcctggcgcc gctgcagggg ccgcacgatg aaggagagca
     6601 gaccgagcat gaccgggacg ccgatgagca cgagcaggcc gagcgggggt gaggcctgca
     6661 ggaggatcac ggcgacgacg acatagctga cgacggcgcc cgcgaaccgc gcgacgacgt
     6721 catacacccc cccgagccgc atggcgtcgg aggcgacggt cgccacgacc tcacccgtcg
     6781 gcacggtgcg gggcagggca gcaccggtgt cggcggcctt ccacccgatg agctgggcgg
     6841 cccgtatcgc ggcgccgagc cagttgcgga cggcgtacat gtgccggaac gacccggaca
     6901 ccgccacgag gacggccagg ccgaagacgg cccccgccca gagccacagc gaccctcggt
     6961 cctcgccgat gagtccgtca tcgatggccc ggctgatcgc ggcgggcagg agcgcctgcg
     7021 agaccatcca gacc
//
LOCUS       DATAIX010000425         1632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_22992, whole genome shotgun sequence.
ACCESSION   DATAIX010000425 DATAIX010000000
VERSION     DATAIX010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22992"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VHM65_06430"
     CDS             <1..533
                     /locus_tag="VHM65_06430"
                     /inference="COORDINATES: protein motif:HMM:NF015426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEX2705377.1"
                     /translation="VGFDLYVRLVGEAVAEFRGEGEAEPVEIRIELPVDAHLPHDYVP
                     GERLRLEMYRKLAAVPDEAGLAEIEAELRDRYGEPPEAVRNLVEVARLRTVARAAGIA
                     DVTLQGAVVRFGPAQLRESQQLRLLRLYPGSLVKEPLRTILVPVPKTARVGGVLLRDT
                     AVLRWASDLLRAVVLG"
     gene            553..1068
                     /locus_tag="VHM65_06435"
     CDS             553..1068
                     /locus_tag="VHM65_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705378.1"
                     /translation="MNRSSTRALLVGAVAALGLGLTLSACSVVDKAHAAAVVDGRVIE
                     ESAVDASVAQLRSVEVTVGPGVVLGMLILAKPVADQVSASGSWKPDAAYNAFLARIPD
                     PTPATVEVVRTNSALSALTADDQKAVLAALRSMTVTVNPRYGSFNASTGQIAPLLPAW
                     IRKPATPVPGS"
     gene            1099..>1632
                     /locus_tag="VHM65_06440"
     CDS             1099..>1632
                     /locus_tag="VHM65_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011068175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEX2705379.1"
                     /translation="MGLTLLLSSPRLPAGVLTRGAWSSLEAATAVYGWPEDAQVVAIT
                     DAGIAVAPPPADEPAGLAAALVAATFTGPVVWVGGADGDPGLTDALALEVSRHTEPGR
                     PAPDIELLLGSHDMVGARLLDLVAVMDRLRESCPWDRKQTHVSLVPYLVEETYEAVEA
                     IESGDRAHLVEELGDVLL"
BASE COUNT          204 a          619 c          584 g          225 t
ORIGIN      
        1 gggtcggttt cgacctctat gtgcgcctcg tcggcgaggc cgtggccgag ttccgcggcg
       61 agggggaggc cgagccggtc gagatcagga tcgagctgcc cgtcgatgcc cacctgccgc
      121 acgactacgt cccgggggag cggctgcggc tcgagatgta ccgcaagctg gccgccgtcc
      181 cggacgaggc cgggctcgcc gagatcgagg ccgagctcag ggaccggtac ggcgagcccc
      241 ccgaggcggt ccggaacctg gtcgaggtgg cccggctgcg gacggtggcc cgggcagcgg
      301 ggatcgcgga cgtcacgctc cagggcgccg tcgtgcgctt cgggccggcc cagctgcgcg
      361 agagccagca gctgcggctc ctgcggctct accccggctc cctcgtcaag gagccgctgc
      421 ggacgattct cgtgcccgtc ccgaagacgg cacgggtggg cggagtcctg ctgcgcgaca
      481 cggccgtcct acgatgggcc agtgatctcc tgcgtgcggt cgtcctcggc tgacgtgacg
      541 aaaggaacca ccgtgaaccg ctcgtcgacc agggcgctcc tcgtgggcgc cgtcgcggcg
      601 ctcggcctcg ggctgaccct cagcgcgtgc tccgtcgtgg acaaggccca cgccgccgcg
      661 gtcgtcgacg ggcgcgtcat cgaggagtcc gccgtcgacg cgtcggtcgc ccagctgcgc
      721 tccgtcgagg tgacggtcgg accgggtgtc gtcctgggga tgctcatcct cgccaagccc
      781 gtcgcagacc aggtgagtgc gtcggggtca tggaagcccg acgccgccta caacgcgttc
      841 ctggcccgga tcccggaccc gaccccggcg acggtggagg tggtgcggac gaactcggcc
      901 ctcagcgccc tgacggccga cgaccagaag gcggtcctcg cggcccttcg ctcgatgacg
      961 gtgacggtca acccccgcta cgggtccttc aacgcgtcca ccggccagat cgcgccgctg
     1021 cttccggcgt ggatccgcaa gccggcgacc cccgtcccgg gctcctgacg gcgacccggc
     1081 cgagacgaca ccgacctcat gggcctgacc ttgctgctgt ccagcccccg cctgccggcc
     1141 ggcgtcctca cccgcggcgc ctggtcctcg ctcgaggcgg cgacggcggt ctacggctgg
     1201 cccgaggacg cgcaggtcgt cgcgatcacc gacgccggca tcgccgtcgc accgcctccc
     1261 gccgacgagc cggccggcct cgccgccgcc ctcgtcgcgg ccaccttcac gggccccgtc
     1321 gtctgggtcg gcggcgccga tggcgacccc gggctcaccg acgcgctcgc gctcgaggtg
     1381 tcgcgccata ccgagcccgg ccgtcccgcg cccgacatcg agctgctcct cggctcccac
     1441 gacatggtcg gcgccaggct cctcgacctc gtcgcggtca tggaccggct gcgcgagagc
     1501 tgcccgtggg accgcaagca gacccacgtg agcctcgtgc cctacctcgt ggaggagacc
     1561 tacgaggcgg tcgaggcgat cgagtccggc gaccgcgccc acctggtgga ggagctcggg
     1621 gacgtgctgc tg
//
LOCUS       DATAIX010000426         2394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_172525, whole genome shotgun sequence.
ACCESSION   DATAIX010000426 DATAIX010000000
VERSION     DATAIX010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172525"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /gene="disA"
                     /locus_tag="VHM65_06445"
     CDS             complement(<1..565)
                     /gene="disA"
                     /locus_tag="VHM65_06445"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HEX2705380.1"
                     /translation="MRATLAAVAPGTDLRDGLERILRGRTGALIVLGHDKTVESICTG
                     GFPLDVEFSATRLRELAKMDGAIVLDKDCTRILRAATQLVPDPTIETSEAGTRHRTAE
                     RVAIQTGLPVVSVSQSMRIVALYVGGRRRVVEDSAAILSRANQALQTLERYKSRLDEV
                     TGSLSALEIEDLVTVRDVASVLQRLEMV"
     gene            complement(632..2032)
                     /gene="radA"
                     /locus_tag="VHM65_06450"
     CDS             complement(632..2032)
                     /gene="radA"
                     /locus_tag="VHM65_06450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX2705381.1"
                     /translation="MASRGTVPRTVRPSGLRCSECGWTTVKWVGRCGECQTWGSVAEV
                     AAVPARRTTAGPAVTAAQRIGEVDLDRSVWEPTGVGEFDRVLGGGLVPGSVVLVAGEP
                     GIGKSTLVLDVAARAARAGRAVLYVTGEESVAQIRMRAERIEAMAASLYLAAETDLAS
                     VLGQIEAVDPAVVVIDSVQTIASAEVDGAAGNVTQVREVAASLIHVAKTRGIAVLLVG
                     HVTKDGSIAGPRLLEHLVDVVIGFEGERHSRLRLVRAVKNRYGPTDEVGCFDLSDVGI
                     VGLADPSGLFLSTRDQLVPGTCVTVTLEGRRPLVAEIQALVAASSGPAPRRVTHGLDP
                     SRVAMVAAVLERRARAPLGSSDSYVSTVGGVRVTEPAADLAVALAMASAMVDRPLAPR
                     AIAIGEVGLAGEIRPVSAVPRRLSEAARLGFTTAFVPPGVIGSGPLPESLRVIEVAQL
                     VEAVGAALTRPVALTG"
     gene            complement(2111..>2394)
                     /locus_tag="VHM65_06455"
     CDS             complement(2111..>2394)
                     /locus_tag="VHM65_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705382.1"
                     /translation="VAAVVDNDATRSTFGQWFRRARPDYDPAAVERFWRRQGWGRERL
                     GIGWDFTSREDFEAVVRIEFAPAMADRILAEDPARTGVDYAVNLWWRRF"
BASE COUNT          345 a          872 c          876 g          301 t
ORIGIN      
        1 ggaccatctc caggcgctgc aggacgctgg cgacgtcgcg gacggtgacg aggtcctcga
       61 tctcgagtgc ggacaggctg ccggtgacct cgtcgagcct ggacttgtag cgctcgaggg
      121 tctgcagggc ctggttggcg cgggacagga tcgcggcgga gtcctcgacg acgcggcgcc
      181 ggccaccgac gtacaaggcg acgatccgca tcgactggct caccgagacg accgggagcc
      241 cggtctggat cgcgacgcgc tcggcggtgc ggtggcgcgt gccggcctcg ctcgtctcga
      301 tcgtcgggtc ggggacgagc tgggtggcgg cgcgcaggat ccgggtgcag tccttgtcga
      361 ggacgatggc gccgtccatc ttggcgagct cgcgcagccg ggtcgcggag aactcgacgt
      421 cgagcgggaa gccgcccgtg cagatcgact cgaccgtctt gtcgtgcccg aggacgatga
      481 gggcacccgt gcgcccacgc aggattcgct cgagaccgtc gcggaggtcg gtgccagggg
      541 cgacagcggc gagggtggcg cgcacgaggt cttcgccgga ggtttccgcg ctgatggtcg
      601 ctcctcgggt gtcggcggct gcgtcgtcat cctagccggt gagggccacg ggcctggtca
      661 gcgcggcgcc caccgcttcg acgagctggg cgacctcgat gacgcgcagg gactcgggca
      721 gggggccgga cccgatcact cccggaggga cgaaggccgt cgtgaacccg agccgggccg
      781 cctcggacag ccgtctcggg accgcgctga ccgggcggat ctcaccggcc agccccacct
      841 cgccgatcgc gatcgcccgt ggtgccagag gccggtcgac catggccgag gccatggcga
      901 gagcgacggc caggtcggct gcgggctcgg tgacccggac cccgccgacc gtcgagacgt
      961 aggagtcgct cgagcccagt ggcgcccggg cgcgacgctc gaggacagcc gccaccatgg
     1021 cgaccctgga cgggtccagg ccgtgggtga cccgccgcgg ggccggcccg ctcgaggcgg
     1081 ccacgagggc ctggatctcg gcgaccagcg gtcggcggcc ctccagcgtc accgtgacac
     1141 aggtcccggg cacgagctgg tcccgggtcg acaggaacag cccgctcggg tcggccagcc
     1201 cgacgatccc cacatcggac aggtcgaagc agccgacctc gtcggtcggg ccgtagcggt
     1261 tcttgaccgc ccggacgagc cgcagccgcg agtggcgctc gccctcgaaa ccgatgacga
     1321 catcgacgag gtgctcgagc agccggggcc ccgcgatcga cccgtccttg gtgacgtgcc
     1381 cgacgaggag cacggcgatg cctcgggtct tggcgacatg gatgaggctc gcggccacct
     1441 cgcggacctg ggtgacgttg ccggcggcgc cgtcgacctc ggcgctggcg atggtctgga
     1501 ccgagtcgat gacgacgaca gccgggtcga ccgcctcgat ctggccgagg acgctcgcga
     1561 ggtcggtctc ggccgcgagg tagagcgagg cggccatcgc ctcgatccgc tcggcccgca
     1621 tccggatctg cgccacggac tcctcgcccg tcacgtagag caccgcacgc ccggcccgcg
     1681 ccgcccgcgc ggccacgtcg agcaccaggg tgctcttgcc gatcccgggc tctccggcca
     1741 cgagcaccac cgagcccggc acgagcccgc cgccgagcac ccggtcgaac tcgcccaccc
     1801 cggtcggctc ccataccgac cgatccaggt cgacctcgcc gatgcgctgg gccgcagtga
     1861 ccgccgggcc ggccgtggtc cggcgagccg gcaccgccgc cacctcggcg accgaacccc
     1921 aggtctggca ctccccgcag cgcccgaccc acttgacggt cgtccatccg cactcggagc
     1981 agcgcaagcc ggagggacgc accgtgcggg ggaccgttcc tcgggacgcc atgtccgtga
     2041 ccctacgagc cggcaccgac agaccaggcc gaggcttgcc gtcatacgct gatggtcaag
     2101 aagcgggggg ctagaagcgg cgccaccaca ggttgacggc gtagtcgacg ccggtccggg
     2161 ccgggtcctc ggcgaggata cggtcggcca tggccggggc gaactcgatc cgcacgaccg
     2221 cttcgaagtc ctcccgtgag gtgaagtccc agccgatgcc gagccgctcc cgcccccagc
     2281 cctggcgccg ccagaaccgc tcgaccgccg cagggtcgta gtccggccgg gcccggcgga
     2341 accactgtcc gaaggtcgag cgggtcgcgt cgttgtcgac caccgccgcg acac
//
LOCUS       DATAIX010000427         2045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_112919, whole genome shotgun sequence.
ACCESSION   DATAIX010000427 DATAIX010000000
VERSION     DATAIX010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112919"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VHM65_06460"
     CDS             complement(<1..1100)
                     /locus_tag="VHM65_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705383.1"
                     /translation="MSDIPGGYDGPLPPALPTVPGVEVSGLASAPGEPPSWAGVDLLT
                     GASVRVRVLSAPADTVELDAARSHAARMTLVRPETVRDHLTRRHVVDLADGTSALVCD
                     TVDGTSLADLLATRGALTAGETVTVLVPLLQIAGDLHAAGSAVGGVGPGQLLVTADGR
                     PVLDVMGPTQESADPRLAAADDVRAVGELAWRLLGGHTGAGEGRPRRREDVLAQAGPV
                     LGPALADALHRDPMRRPRADELLAGLFAAQAPEPLGLDGPPAGPSEVPLTRAERRRVS
                     ASAAKHALPGGAATSRPSVPAWVRRSAGPTRPMVPGAWVRPGALVLVGALVVAVVAVA
                     ASRMWPDPLSSPEVAAVRASATTASGTVAADPV"
     gene            1224..1958
                     /locus_tag="VHM65_06465"
     CDS             1224..1958
                     /locus_tag="VHM65_06465"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX2705384.1"
                     /translation="MSDREPTILATSGGYVAHDRLRFAFGPLLHYAVDLSGAHGRRPR
                     VTHIGTAMGDQRALNDDMSEAARVAGFDLTTLALFPMPNLEDMEGHLLAQDVVWVNGG
                     SVANLLAVWRVHGLDAIVRRVWEAGVVLAGVSAGSICWYAGGPTDSFGPSLRVVTNGI
                     GLLPYGNGVHYDSEAQRRPLVHSLVADGTLPQTHCTDDGVGLLYRGTTLVEAVSEISG
                     RSAYVVRRDGDRAVEERREPRLLPLG"
BASE COUNT          258 a          785 c          741 g          261 t
ORIGIN      
        1 accggatccg cagcgacggt gccggaggcc gtcgtcgccg aggcccggac ggccgccacc
       61 tcgggcgagg acaacgggtc cggccacatc cgcgaagccg cgacggccac gacagcaacg
      121 acgagtgcgc cgacgagcac caaggcccca gggcggaccc acgcgccggg gaccatcggt
      181 cgggtcgggc ccgccgaccg tctcacccag gcggggacgc tcggacgcga ggtggcggct
      241 cccccgggca gggcgtgttt ggcggccgat gcggacaccc ggcggcgctc ggcccgggtg
      301 aggggcacct cgctcggacc ggccgggggg ccgtccagcc ccagcggctc cggggcttgc
      361 gccgcgaaca gcccggcgag cagctcgtct gcccgcggcc gcctcatcgg gtcccggtgg
      421 agcgcgtcgg cgagcgccgg gcccagcacc ggcccggcct gcgccagcac gtcctcgcgt
      481 cggcgcggcc ggccctcacc cgcgccggtg tggcccccca gcagtcgcca ggcaagctcc
      541 cccaccgcgc ggacgtcgtc ggcggcggcg agcctggggt cggcggactc ctgcgtagga
      601 cccatgacgt cgagcacggg acgtccgtcg gcggtgacga gcagctgccc aggcccgact
      661 ccgccgaccg ccgagcccgc ggcgtgcagg tcgccggcga tctgcaggag cggcacgagc
      721 acggtgaccg tctcaccagc ggtcagtgcc ccccgcgtgg ccaggaggtc agccaggctc
      781 gtcccgtcga cggtgtcaca caccagagca ctggtgccgt cggcgaggtc gacgacatgc
      841 cggcgggtca ggtggtcccg gaccgtctcg ggccggacca gcgtcatgcg cgcggcatgg
      901 ctgcgggcag cgtcgagctc gaccgtgtcg gcgggcgccg agagcacccg cacccgcacc
      961 gacgctccgg tgagcaggtc gacgccagcc cagctcggcg gctcacccgg cgcactcgcg
     1021 agcccgctca cctcgacccc cgggacggtg ggcagcgcgg ggggcagcgg cccgtcatac
     1081 ccgccaggta tgtcgctcat cctcacccct gctcatcggc acccctgctc gctgcgccct
     1141 cgtgtggtcc gcaccccagt ctggccgccc ggcgcctgtc gtgcccgccg ttgtccacag
     1201 cctgcgcccg gtagcctcag cggatgagtg accgggagcc caccatcctt gcgacatcgg
     1261 gcggctacgt cgctcacgac cggctgcgtt tcgccttcgg cccgctgctg cactatgccg
     1321 tcgacctgtc cggcgcgcac gggcgccgtc cccgggtcac ccacatcggg actgccatgg
     1381 gtgaccagcg ggcgctcaac gacgacatgt cagaggcggc gcgcgtcgcc gggttcgacc
     1441 tgaccaccct cgcgctgttc cccatgccca acctcgagga catggaaggg cacctcctcg
     1501 cgcaggacgt cgtgtgggtc aacggtgggt cggtggccaa cctgctcgcg gtatggcgtg
     1561 tccacggtct cgatgccatc gtgcgccgcg tctgggaggc gggcgtggtc ctcgcagggg
     1621 tctccgcggg gtcgatctgc tggtatgccg gtggcccgac cgactcgttc gggccctcgc
     1681 ttcgggtcgt gaccaacggg atcggcctgc tcccttacgg caacggcgtc cactacgaca
     1741 gcgaggcgca gcgccggcca ctggtgcact ccctcgtcgc cgatggcacg ctgccccaga
     1801 cgcactgcac cgacgacggc gtggggctcc tctaccgggg gaccacactg gtcgaggcgg
     1861 tgagcgagat ctccggcagg agcgcgtatg tcgttcgccg ggacggggat cgcgccgtcg
     1921 aggagcgccg tgagccccgg ctgcttccgc tggggtgacc ccgccggggt gccgcccgcc
     1981 gtggcggacc ggctacctcg cgccccgccg acgccggccg gggtctacgt catcacgggc
     2041 ggaac
//
LOCUS       DATAIX010000428         3246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_278440, whole genome shotgun sequence.
ACCESSION   DATAIX010000428 DATAIX010000000
VERSION     DATAIX010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278440"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1393)
                     /locus_tag="VHM65_06470"
     CDS             complement(<1..1393)
                     /locus_tag="VHM65_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2705385.1"
                     /translation="MSAPEGVKTAEEKAAEARRGPRRQPGPPHMQMGMPTEKSASFGP
                     SAKRLLGWLAPEKGKTLLVVLIMIASVALSSIGPKILGQATNLIFAGFLGAKMPFGAS
                     KAAVIDQLRAQGQTQMADLLSGVDFTPGLGIDFAAVGRVLLTVMVIYVVAAILMWAGS
                     WLLTGIVQRTMYRLRDAVETKLGRLPLAYFDNQPRGELLSRVTNDIDNISMSLQQTIS
                     QLLSSLLTVIAVLGIMFWISWLLALIALVTIPLSMVVTTLIAKRSQKLFVQQWAATGE
                     LNGIIEETFTGHQLVKVFGRQREVESTFEAKNDELYRSAFGAQFISGIIMPTMMFVGN
                     LTYVAVAVAGGLKVASGQISLGDVQAFIQYSRQFTQPLTQVASMANLLQSGVASAERV
                     FEVLDATEQEPESLHPVAIENPHGEVRLEDVSFSYTQDQPLIEHLDLVVDPGHTVAIV
                     GPTGAGKTTLVNLV"
     gene            complement(1390..3126)
                     /locus_tag="VHM65_06475"
     CDS             complement(1390..3126)
                     /locus_tag="VHM65_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2705386.1"
                     /translation="MLWRLIRSGMAPHRTAISLIVALQLASTIASLYLPNLNGDIIDQ
                     GVTKGDTGYIMRTGGVMLAISLVQIAASVYAVYLSARTSMAFGRDTRAQVFHRVGEFS
                     AREVAQFGAPTLISRSTNDVQQIQMVVFMALSMMVSAPIMMVGGVIMALRTDVGLSWL
                     VAVAVPVLAITVALVLRQMLPKFRVMQDSVDSVNRILREQITGIRVVRAFVREPFERT
                     RFAVANGTLTGSALGVGRLMALVFPIVMLILNASTVGVLWFGAHRVEAGDMQVGALTA
                     FMSYLIQILMSVMMATMMSMMIPRAAVSAERITAVLDTQTSVVLPAHPDTHLATPAAV
                     TFDHVDYHYPGAERAVLDGVSFEARPGETTAVIGSTGSGKTTLLGLIPRLFDATGGRV
                     LVGGVDVRNALPDALWSHLGLVPQKPYLFSGTVATNLRYGKGDASDDELWEALRIAQA
                     EGFVREMPGGLDAPIAQGGTNVSGGQRQRLAIARALVRRPDVYLFDDSFSALDLTTDA
                     RLRSALRPVTRNATVIVVAQRVSTIIDAEQIIVLDDGHVVGAGRHTDLLESCPTYQEI
                     VGSQRAAQEVPA"
BASE COUNT          555 a         1022 c         1172 g          497 t
ORIGIN      
        1 tgacgaggtt gacgagcgtc gtcttgcccg ctccggtggg gcccacgatc gcgaccgtgt
       61 gaccggggtc tacgacgagg tcgaggtgct cgatgagtgg ctgatcctgc gtgtaggaga
      121 acgagacgtc ctcgaggcgc acttccccgt gggggttctc gatcgccacg gggtgcaggg
      181 actcgggctc ctgctcggtt gcgtcgagga cctcgaagac ccgctcggcc gaagcgaccc
      241 ctgactgcag caggttggcc atcgaggcga cctgtgtgag cggctgggtg aactggcgcg
      301 agtactggat gaacgcctgg acgtcaccga gggagatctg cccggaggcg accttgagcc
      361 cgccggcgac ggcgacggcg acgtaggtga ggttcccgac aaacatcatc gtcggcatga
      421 tgatgccgct gatgaactgt gcgccgaacg ccgaacggta gagctcgtcg ttcttggcct
      481 cgaacgtcga ctcgacctca cgctgacgcc cgaagacctt gaccagctgg tgcccggtga
      541 aggtctcctc gatgatcccg ttgagctcgc ccgtggccgc ccactgctgg acgaacagct
      601 tctggctgcg cttggcgatc agggtcgtga cgaccatcga cagcgggatc gtcacgaggg
      661 cgatgagcgc cagcagccac gagatccaga acatgatccc caggacggcg atgacggtga
      721 gcaatgagct gagcagctga gagatggtct gctgcagaga catcgagatg ttgtcgatgt
      781 cgttggtcac ccggctgagc agctcgccgc gaggctggtt gtcgaagtac gccagcggca
      841 gccgaccgag cttggtctcg acggcatcgc gcaggcgata catcgtgcgc tggacgatgc
      901 cggtgagcag ccagctgccc gcccacatga ggatcgcggc cacgacgtag atcaccatga
      961 ccgtgagcag cacgcggccg accgcggcga agtcgatgcc gaggccgggc gtgaagtcga
     1021 cgccagagag caggtcggcc atctgggtct ggccctgggc tcgcagctgg tcgatgaccg
     1081 ccgccttgga ggcaccgaag ggcatcttcg cgccgaggaa gccggcgaag atgaggttgg
     1141 tcgcctggcc gaggatcttc ggcccgatcg agctcagcgc gacgctggcg atcatgatga
     1201 ggacgacgag cagcgtcttg cccttctccg gggccagcca gccgagcagt cgcttcgcgg
     1261 aggggccgaa cgaggccgac ttctcggtcg gcatacccat ctgcatgtgc ggcgggccgg
     1321 gctgccggcg ggggccgcgc cgggcctcgg ccgccttctc ctcggccgtc ttcaccccct
     1381 ctggcgcact catgccggca cctcctgcgc ggcgcgctgg gacccgacga tctcctggta
     1441 tgtcggacag gattccagca ggtcggtatg ccgtccggcc cccacgacgt gtccgtcgtc
     1501 gaggacgatg atctgctcgg catcgatgat cgtcgaaacc cgctgtgcga caacgatgac
     1561 ggtggcgttg cgggtgaccg ggcgcagtgc cgaacgcagc cgcgcgtcgg tcgtcaggtc
     1621 gagcgccgag aacgagtcgt cgaagaggta gacatcgggt cggcgcacga gggcccgggc
     1681 gatcgcgaga cgctggcgct gaccgccgga gacattggtc ccgccctgag cgatgggcgc
     1741 atcgagcccc ccaggcatct cgcggacgaa gccctcggcc tgggcgatcc gcagggcttc
     1801 ccacagctcg tcgtcggacg cgtcgccctt gccgtagcgc aggttcgtcg cgaccgtgcc
     1861 ggagaagagg tacggcttct gcggcaccag gcccaggtgc gaccagaggg cgtcgggcaa
     1921 ggcattgcgc acgtcgaccc ctccgacgag cacccggccg ccggtcgcgt cgaacagccg
     1981 ggggatgagc ccgagcaggg tcgtcttgcc ggaaccggtc gaaccgatga cggcggtcgt
     2041 ctcaccgggg cgcgcctcga aggagacccc gtcgagcacc gcccgctcgg cacccgggta
     2101 gtggtagtcg acgtggtcga aggtgaccgc ggccggagtg gccaggtggg tgtcggggtg
     2161 cgcaggcagg accaccgacg tctgggtgtc gagcaccgcg gtgatgcgct cggccgagac
     2221 ggcggcccga gggatcatca tcgacatcat ggtggccatc atcacggaca tgaggatctg
     2281 gatgaggtag gacatgaagg cggtgagcgc ccccacctgc atgtcgccgg cctcgacccg
     2341 gtgcgcgccg aaccagagca ctccgacggt ggaggcgttg aggatgagca tgacgatcgg
     2401 gaagaccagc gccatgagcc ggccgacccc gagggccgac ccggtgagcg tgccgttggc
     2461 cacggcgaaa cgcgtgcgct cgaacggctc gcgcacgaat gcccgcacca cgcggatgcc
     2521 ggtgatctgc tcgcgcagga tccggttgac cgagtcgacc gagtcctgca tgaccctgaa
     2581 cttcggcagc atctggcgca gcacgagcgc cacggtgatc gcgaggaccg gcacggcaac
     2641 ggcgacgagc caggacaggc cgacatcggt gcgcagggcc atgatgacgc cgccgaccat
     2701 catgatcggt gcgctcacca tcatcgacaa ggccatgaag acgaccatct ggatctgctg
     2761 gacgtcgttg gtggaccggc tgatgagcgt cggggccccg aactgggcga cctcccgggc
     2821 cgagaactcg ccgacccggt ggaagacctg ggcacgggtg tcgcgcccga aggccatcga
     2881 cgtgcgagcg gagaggtaga cggcatacac cgaggcggcg atctggacca ggctgatcgc
     2941 gagcatgacc ccaccggtgc gcatgatgta gccggtgtcc cccttcgtca ccccctggtc
     3001 gatgatgtcc ccgttgaggt tgggcaggta gagcgaggcg atcgtgctcg cgagctggag
     3061 cgcgacgatg agggagatcg cggtgcggtg tggggccatc ccactgcgga tcagtcgcca
     3121 gagcatgtgt gtccttcggt cggtagtcca gatgcccatg gtgcgggccc acgtggggtg
     3181 agggtcaagt cgttaacggg ccggtcatgt tgtgtcgagc ccgcggcgag cactccgtgc
     3241 aggagg
//
LOCUS       DATAIX010000429         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_218787, whole genome shotgun sequence.
ACCESSION   DATAIX010000429 DATAIX010000000
VERSION     DATAIX010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218787"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2188)
                     /gene="cphA"
                     /locus_tag="VHM65_06480"
     CDS             complement(<1..2188)
                     /gene="cphA"
                     /locus_tag="VHM65_06480"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX2705387.1"
                     /translation="MAADRPDPSGPPAAELRIVESRVYRGPNIWSYEPAIHLVLDLGV
                     LEGYPSDTLDGFTDRLLALLPGLGKHTCSRGHPGGFVERLREGTWLGHVTEHVALQLQ
                     QEAGHDHRRGKTREVKDRPGHYNIIFGYADESVGLEAGRLAVRIVNDLVQAEDGFDFP
                     AELNVFLTRAERTAFGPSTAAILEEAASRDIPYIRLNAASFVQLGQGVHAQRIRATMT
                     SKTGALAVDIAGDKDMTTKLLASAGLPVPRSESVRTLAGALSAARRVGFPVVLKPLDG
                     NHGRGVCLNLRSDGDVEKAFHVAKSESRRGWVIVESLVIGRDYRCLIVGGRMQAIAER
                     VPAHVLGDGTRTVGELVDITNADPRRGVGHEKVLTKIKVDAGALEVLSGQGYDLEDVP
                     PKDEMVRLALTGNMSTGGISIDRTFDAHPDNVEIAEEAARMIGLDVAGIDFICPDIAA
                     PVRETGGAICEVNAAPGFRMHTHPTVGVPQFIAKPVVDLLFPPGSPSRVPIIAVTGTN
                     GKTTTARMIAHIFKGLGRKVGMTSTDGIVIDERLVIKADASGPKSARMVLQNPRVDLA
                     VMEVARGGILREGLGYDRNDVAVVTNVAPDHLGLRGISTVEELANVKAVTVEAVPRDG
                     FAVLNADDPLVRRMRRRCSGGIVWFSLQPPGTRVRDFIDDYCRRGGRAVVLDPTDKGD
                     LIVIQHGRRRMQLAWTHLLPATFGGTAKFNVANALAAAGAAFAAGAP"
BASE COUNT          324 a          745 c          839 g          346 t
ORIGIN      
        1 ggggggctcc cgcggcgaag gccgctccgg cggcggccag ggcgttggcg acgttgaact
       61 tcgccgtccc gccaaaggtt gccgggagca gatgggtcca cgcgagctgc atccggcgac
      121 ggccgtgctg gatgacgatc aagtcgccct tgtcggtggg gtcgagcacg acggctcgcc
      181 caccgcggcg gcagtagtcg tcgatgaagt cgcgcacccg ggtgccgggc ggctggaggg
      241 agaaccacac gatcccgccc gagcaccgcc gtcgcatgcg gcgcacgagc gggtcgtcgg
      301 cgttgaggac cgcaaagccg tcacggggca ccgcctcgac cgtgacggcc ttgacgttgg
      361 cgagctcctc aacggtcgag attccccgca agccaaggtg gtcgggtgcg acgttggtca
      421 cgacagcgac gtcgttgcgg tcatagccca gtccttcacg caggatcccg ccgcgggcca
      481 cctccatcac cgcgaggtcg acgcgcgggt tctgcaggac catccgagcc gacttggggc
      541 ctgacgcgtc ggccttgatg acgagccgct cgtcgatgac gatgccgtcg gtcgacgtca
      601 tgccgacctt gcgtccgagg cccttgaaga tgtgggcgat catccgcgcg gtcgttgtct
      661 tgccgttggt gccggtcacc gcgatgatcg gcacccgcga cggcgagccc ggtgggaaga
      721 gcaggtcgac gaccggcttg gcgatgaact gcgggacgcc gaccgtcggg tgcgtgtgca
      781 tccggaaccc gggcgcggcg ttgacctcgc agatcgcacc gccggtctcg cggacggggg
      841 cggcgatgtc ggggcagatg aagtcgatcc cggcgacgtc gaggccgatc atccgggccg
      901 cctcctcggc gatctcgacg ttgtccgggt gggcgtcgaa ggtccggtcg atcgagatgc
      961 cgccggtcga catgttgccg gtcagcgcga gcctgaccat ctcatccttc ggcgggacgt
     1021 cctccaggtc gtacccttgg ccggagagca cctccagggc gcccgcgtcg accttgatct
     1081 tggtcagcac cttctcgtga ccgacccccc ggcgtgggtc ggcgttggtg atgtcgacga
     1141 gctcacccac cgttcgggtg ccgtcgccga ggacgtgggc gggcacccgc tcggcgatcg
     1201 cctgcatccg gccgccgacg atgaggcagc ggtagtcgcg tccgatgacg agggactcga
     1261 cgatgaccca gccgcggcgc gactcggact tggcgacgtg aaacgccttc tcgacgtcgc
     1321 cgtcgctgcg caggttgagg cagacccccc gtccgtggtt gccgtcgagc ggcttgagga
     1381 cgaccgggaa cccgacccgc cgggcggccg acagggcacc agcgagggtc cggaccgact
     1441 cgctgcgagg caccggcagg ccggccgagg caagcagctt ggtcgtcatg tccttgtcgc
     1501 cggcgatgtc gacagccagg gccccggtct tggaggtcat cgtggcccgg atgcgctggg
     1561 cgtggacgcc ctggccgagc tggacgaagg acgccgcgtt gagccggatg taggggatgt
     1621 cccggctcgc ggcctcctcg aggatcgccg cggtggacgg gccgaaggcg gtgcgctcgg
     1681 cccgggtgag gaagacgttg agctcggccg ggaagtcgaa cccgtcctcg gcctggacga
     1741 ggtcgttgac gatccgcacc gcgagtcgtc cggcttcgag gccgaccgac tcgtcggcat
     1801 acccgaagat gatgttgtag tgcccgggcc ggtccttgac ctcgcgcgtc ttgccgcggc
     1861 ggtggtcgtg cccggcttcc tgctgcagct gcagcgcgac gtgctcggtg acgtgcccga
     1921 gccacgtccc ctcgcggagc cgctcgacga agccgcccgg atgcccgcgt gagcacgtgt
     1981 gcttgcccag ccccggcagc agggcgagga gccggtcggt gaacccgtcg agggtgtcgg
     2041 aggggtagcc ctcgaggacg ccgaggtcga ggacgaggtg gatcgccggc tcgtatgacc
     2101 agatgttggg cccgcggtag acccgggact cgacgatacg aagctccgcg gcgggcggac
     2161 cggacgggtc gggacggtct gcggccatgt ggcaatcctg tcaggtgcca gtccgactca
     2221 agaccaggcg caggtcaggc tcagggtcag gctc
//
LOCUS       DATAIX010000430         2805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_172708, whole genome shotgun sequence.
ACCESSION   DATAIX010000430 DATAIX010000000
VERSION     DATAIX010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172708"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VHM65_06485"
     CDS             complement(<1..586)
                     /locus_tag="VHM65_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705388.1"
                     /translation="MSGPSSGTPPRRLPSVRRTSSMDLLRPSGPAGPMVIAGRARDLL
                     TAPAGQARVLATGLLSATIGTDGRVARLVTYPDGARAVALVGRPAGTGFRTAVWRTLR
                     EHYDSGTPLHLLLDEVPVGLVISGYAARRAAPRGLPRRGRRLDVCAGWASDGRAASVL
                     SASGSPPDLATPGAPSLAVDDPRGWHTMAPLPVFA"
     gene            complement(658..1542)
                     /locus_tag="VHM65_06490"
     CDS             complement(658..1542)
                     /locus_tag="VHM65_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEX2705389.1"
                     /translation="MTATTKESPHGATAREPGRTAAFFDLDGTLIPGSANIPLAKAAF
                     GMGLVSKRELALDLAHGLTFILFGASDDRSATVRDRILHAVAGHPAAEVEALADGFLD
                     DLIATITPAMRAVLAGHTSRGHDLVLVSASPTEIVQRFAAAAGMSYGAGTLSERDGQG
                     RYTGKLAGPFCYKEGKAQVILELAAEHGYDLAVSYAYSDSISDLPMLRIVGNPVTVNP
                     DTELRRLALTEGWQILDTSRLRGGIASVIDAARVSLRATWHAVRVSGRALSGAAQSTA
                     RTTVRGVSRATSRTVDET"
     gene            1820..2575
                     /locus_tag="VHM65_06495"
     CDS             1820..2575
                     /locus_tag="VHM65_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEX2705390.1"
                     /translation="MATGEDSIPHGLGWLSAREATRLADARFTKRRTEYLLRRWAGKR
                     TVAQAAGLPVDDETLSRIELLNRLTGAPYVEIDGLSAGVDISLTDRAGWAVALVGEPG
                     SAEAGSLGVDLEIVEPRTAGFVRDFLTEPEQAYVHDRRESSGADGWNAAANLLWSAKE
                     AALKVLRVGLRADTRTVEVVIDHTGRPDGWAPMTVTGQHGAVFPGWWRRDGVFLLTIA
                     YRAPSPPPALLAGGADLARAIPTHSWLANPTVR"
BASE COUNT          384 a         1009 c         1045 g          367 t
ORIGIN      
        1 tcgcgaagac cggcaggggc gccatcgtgt gccacccgcg tgggtcgtcc acggccagcg
       61 agggcgcccc gggtgtggcc agatccggcg gggacccgct cgccgacagg accgaagcgg
      121 cccggccgtc gctggcccag ccggcgcaga cgtcgaggcg gcggccgcgc cggggcagtc
      181 cgcgcggcgc ggcgcgacgg gcggcatacc cggagatgac caggccgacc ggcacctcgt
      241 cgagcaggag gtgcagcggg gtcccgctgt cgtagtgctc acgcagcgtg cgccagaccg
      301 cggtccggaa cccggtcccg gcggggcgcc cgaccagcgc aacggcgcgg gcaccatcgg
      361 ggtatgtgac gaggcgcgcg actcggccat cggtcccgat cgtcgccgag agcaggccag
      421 tcgccaggac ccgggcctgc ccggccggag cggtcagcag gtcccgggcc cggccggcga
      481 tgaccatcgg gccagccggg cccgacgggc ggagcaggtc catgctgctc gtgcggcgga
      541 ccgacggcag gcgacgaggg ggcgtgccgc tcgacgggcc ggacatggct ggcaacagcc
      601 cagccgggcg ggacagggca ccctcagggc ggaacgggcc agggctgacc ggtgccctca
      661 cgtctcgtcg acggtacgac tcgtcgcgcg agagacacct cggacggtcg tccgcgccgt
      721 gctctgggca gcccccgaca gggccctgcc gctgacccgc accgcatgcc aggtggcgcg
      781 cagcgacacc cgggccgcgt cgatgaccga ggcgatgccg ccgcgcagac ggctggtgtc
      841 gaggatctgc cagccctcgg tcagggcgag gcggcgcagc tcggtgtcgg ggttcacggt
      901 caccgggttc ccgacgatgc gcagcatcgg caggtcgctg atcgagtcgg agtaggcata
      961 gctcaccgcg aggtcgtagc cgtgctcggc agcaagctcg aggatgacct gcgccttgcc
     1021 ttccttgtag cagaacggcc cggccagctt gccggtgtag cggccctgac cgtcacgctc
     1081 ggacagcgtc cccgccccgt aggacatccc cgccgcggcg gcgaagcgct ggacgatctc
     1141 ggtcgggctg gctgacacga ggacgaggtc gtggccgcgc gacgtgtggc cggcgaggac
     1201 ggcgcgcatc gccggggtga tcgtcgcgat gaggtcgtcg aggaagccgt cggcgagggc
     1261 ttcgacctcg gcggccgggt gtcccgcgac cgcgtgcagg atgcggtccc gcacggtggc
     1321 ggagcggtcg tcgctcgccc cgaagaggat gaaggtcagc ccgtgagcca ggtcgagcgc
     1381 gagctcccgt ttgctgacca gacccatgcc gaacgccgcc ttggccagcg ggatgttcgc
     1441 cgaccccggg atcagcgtcc cgtcaaggtc gaagaatgcc gcggtccgtc caggctcgcg
     1501 cgcggtcgca ccgtggggcg actccttcgt cgttgccgtc accgcaggtt ctcctatcgg
     1561 actgagcccg tggatgtcgc cactccagcc gggacggcga cagggtccca ttgtgcggcc
     1621 ccggcagcgt cttgaccact gcccgaggtc ccgtgacgca gattacggtg cacgcgcgcg
     1681 agggcagcca tggctgcgat cctctcgcag gccggctctg cgcgcacctc tttgcggacc
     1741 cggtatggcg cacacccgcc gctcgccgga gaatgggcac ccggccgggt ggctgaccag
     1801 gaggtaggac atgcgctgga tggcgacagg ggaggactcg atcccccatg ggcttgggtg
     1861 gctctccgcc cgcgaggcca cgcgtctggc tgacgcccgc ttcaccaagc gccggaccga
     1921 gtacctcctg cgccggtggg caggcaagcg aacggtggct caggccgccg gactcccggt
     1981 cgatgacgag acgctctcgc ggatcgagct gctcaaccgg ctcacggggg cgccgtatgt
     2041 cgagatcgac ggcctctccg ccggtgtcga catctccttg accgaccggg cgggctgggc
     2101 cgtcgcactc gtcggcgagc cgggcagcgc cgaagccggc tcgctcggcg tcgacctcga
     2161 gatcgtcgaa ccccggaccg ctggcttcgt ccgggacttc ctcaccgagc cggaacaggc
     2221 ctacgtccac gaccggcgag agtcgtccgg tgcggacggc tggaatgccg cggcgaacct
     2281 gctgtggtcg gccaaggagg ctgccctcaa ggtcctgcgg gtcgggttgc gggccgacac
     2341 ccgcaccgtc gaggtggtca tcgaccacac cgggcgcccc gacggatggg cgccgatgac
     2401 ggtgaccggc cagcacggcg cggtcttccc cggctggtgg cgccgcgatg gggtcttcct
     2461 gctgacgatc gcctaccgcg cgccgagccc gccgccggcg ctgcttgccg gcggcgcgga
     2521 cctggcccgc gccataccga cgcattcgtg gctcgccaac ccgaccgtcc ggtgatgccg
     2581 accccggaat ggggacccga gccccgggcg ggctgggcag tgtcgggctg ccccccggcg
     2641 cagaccttgg ctcacggcac tacggctgca accgtcgcgt cgacacccac cctgcgcgcc
     2701 gaccgcgctg accagccgtg ggccgtgccc cggcgggcta tccgggcagc tgcgcggtca
     2761 ccttggccat gccgcgctcc agcgcatcga tgatgtaggg cctgt
//
LOCUS       DATAIX010000431         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_53459, whole genome shotgun sequence.
ACCESSION   DATAIX010000431 DATAIX010000000
VERSION     DATAIX010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53459"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..135)
                     /locus_tag="VHM65_06500"
                     /pseudo
     CDS             complement(1..135)
                     /locus_tag="VHM65_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(292..513)
                     /locus_tag="VHM65_06505"
     CDS             complement(292..513)
                     /locus_tag="VHM65_06505"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2705391.1"
                     /translation="MTNETRNIGGVDSHKDTIHVAVITELGQPVADREFPTTTAGYRR
                     AVAWLIEHGPLAAVGVEGTSSHGGVRWSV"
     gene            complement(844..1086)
                     /locus_tag="VHM65_06510"
     CDS             complement(844..1086)
                     /locus_tag="VHM65_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705392.1"
                     /translation="MFVQHHGGAKDDGEVHERVAQLGLHLGPAKDCLGVDGVRVIAPR
                     GLDPGEQLFIGSRLRGAAPTCVEEPEVHDPVQPRRR"
     gene            1085..1459
                     /locus_tag="VHM65_06515"
     CDS             1085..1459
                     /locus_tag="VHM65_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX2705393.1"
                     /translation="MQGLTSLEVATVQGIALPAAKQRLRRGRARLVSLLADDDDRRLA
                     MKGVPMSRWEARSLVGDYLDDELSARERALLEGHLASCPTCPGLYASIVGVSAAVGRL
                     RDPDSVVPPDIAQRLRGGSVAD"
     gene            complement(1513..1968)
                     /locus_tag="VHM65_06520"
     CDS             complement(1513..1968)
                     /locus_tag="VHM65_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2705394.1"
                     /translation="MTRRPTAAVALAAVLAATLVTSACSASSDGTVAVSAGPTASAAP
                     TSGAELGAAEFAAALKRPGTTIVDVRTPAEYAEGHLPGAVNLDVSSPDFAAQLATLDP
                     SAPYAVYCHSGNRSGVAVTAMVEQGFTDAYHLQGGLGAWQQAGGEVVTG"
BASE COUNT          296 a          722 c          697 g          315 t
ORIGIN      
        1 ctcgtagccc aggtccgagg tgagctcggc gtccatcccg cgttcgagga cggccttgag
       61 catctcgggc agcagcccgc ccttgccggt caggttcagc ccgtcctcat cgacccgggc
      121 cagcagctcg tcgatcagct cgtcggagaa cagctccttg cgtatcgctc tggccgtcgg
      181 aaccggcgcc ccagcccagg cgtgctcctc gtccagcccg aacgccgagc cgtccatctt
      241 cttcgtatcg gtcatgctcg cagtgaactc cttcatcatc agacccaccc cttacacaga
      301 ccatctgaca cccccgtggc tgctggtgcc ctcgaccccg acggccgcaa gagggccgtg
      361 ctcgatcagc cacgccaccg cccgccggta gccggcggtc gtggtgggga actcccgatc
      421 cgcgaccggc tgacccagct cggtgatgac cgcgacgtgg atggtgtcct tgtgcgagtc
      481 gaccccgcca atgttgcgag tctcgtttgt catgatggtg ctgcccttgc gatccaggtg
      541 ggtcggcacc ggccggaccg gtcagacagg acatgcacca ggcccgcgca ccgggccagg
      601 ctcgtattag gtcatgcccg accggccgtt gccgtgacaa cagcgcgagg gccggactga
      661 tcagtccatc ggcacccagc agggcgccag tcaggcaaag ggtcacgacc cccgcgcagc
      721 cataccagca tgtatgtcag gtatggcgtg tacctcaccc ggaacccggt cgacgccgac
      781 gaccttgtgc aggacaccct tgagcgtgcc gtcgcccgcg gcgacacctt ccgtggcgac
      841 agttcaccga cgacgtggct gcaccgggtc atgcacctcc ggttcctcga cacacgtagg
      901 cgccgcaccc cggagccgac tcccgatgaa gagctgctcg cccgggtcga ggccgcgtgg
      961 cgcgatgacg cgtacaccgt cgaccccgag gcagtccttg gccgggccga ggtgaaggcc
     1021 gagctgcgcg acgcgctcgt gcacctcccc gtcatccttc gctccgccgt ggtgttgcac
     1081 gaacatgcag ggcctcacct cgctcgaggt cgcgaccgtc cagggaatcg ccctcccggc
     1141 cgccaaacag cggctgcgcc gtggccgcgc acggttggtc agtctgctcg ccgacgacga
     1201 cgaccgtcgc ctggccatga agggagtgcc gatgagccgc tgggaagccc gctcactcgt
     1261 cggcgactac ctcgacgacg agctcagcgc gcgcgagcgc gccctgctcg aaggtcatct
     1321 cgcctcgtgt ccgacctgcc cgggcctgta cgcgagcatc gtcggggtgt cggccgcggt
     1381 cggccggctg cgcgaccctg actcggtggt acctccggac atcgcccagc gcctcagggg
     1441 cggctcggtc gcggactaag acccggcggc cgccaacgag caggttctgc actggcctaa
     1501 cccggcgtcg cgtcagccgg tcacgacctc gccgccggct tgctgccagg cgccgagccc
     1561 gccctggagg tggtaggcgt cggtgaagcc ctgctccacc atggcggtca ccgcgacccc
     1621 ggagcgattg cccgagtggc agtacacggc atacggcgcg ctcgggtcga gggtggcgag
     1681 ctgggcagcg aagtcgggcg aggatacgtc gaggttgacg gcgcccggca ggtgcccttc
     1741 cgcgtactcc gccggagtgc gcacgtcgac gatggtcgtg ccggggcgct tgagcgcggc
     1801 cgcgaactcg gccgcgccga gctctgcgcc gctggtcggg gcggccgatg ccgtgggccc
     1861 ggcagagacg gcgacggtgc cgtccgagga ggcggagcag gctgatgtga cgagtgtcgc
     1921 ggccaggact gcggcgaggg cgacggcggc agtggggcgg cgggtcacaa cggttctcct
     1981 ggggttgcgg ttgtcggatc tggtgtgcgc aggtactcgt ggtcatcgag
//
LOCUS       DATAIX010000432         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_172735, whole genome shotgun sequence.
ACCESSION   DATAIX010000432 DATAIX010000000
VERSION     DATAIX010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172735"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VHM65_06525"
     CDS             complement(<1..391)
                     /locus_tag="VHM65_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEX2705395.1"
                     /translation="MTSPMMWYLNRGTGLVLLVLFTLTTVGGVLSTSRATPRWWPRFL
                     TQGLHRSLGLLSVILLVLHVATAVVDEYVDIRWWQALLPFGATYKAAFLSLGTLALDL
                     TLAVVVTSLLRGRLSVGAWRAVHLTSYV"
     gene            complement(388..1362)
                     /locus_tag="VHM65_06530"
     CDS             complement(388..1362)
                     /locus_tag="VHM65_06530"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX2705396.1"
                     /translation="MSRWAALGTYVYLAVGDPTASDSAQALARDVLAEVDLSCSRFRD
                     DSDLVRANAQAGRWVDVSPVLAGAVVVALEAARETGGLVDPTLGLLLRDAGYDRTFAL
                     LGGATDEPAALPTMSRPQAWRDVEVDPGGRLCVPTGVALDLGATGKAYAADLVAATVV
                     ERLRIPTVVSIGGDVRVEAPSGDDRDWPIEIAETAADLTGRATGRDPGTDPPVVLLRS
                     GGLATSTVRARRWVRGGRHWHHVFDPRTGTPAAGPWRTITAAGHTCSAANTATTAALV
                     GGDDALAWLEARAVAARLVGTDGTVHVTPGWPDSRESSQTASLPLEIR"
     gene            complement(1400..1822)
                     /locus_tag="VHM65_06535"
     CDS             complement(1400..1822)
                     /locus_tag="VHM65_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705397.1"
                     /translation="MKTALTPLQRNRFRHVLTALTGLATAAAVAAVGAATAVAAKTTE
                     DAAAARAAQAQTKGAAEAAAQTAAQAAVPLLAKRPTRTVVTTTVVDGSTRSGSAAVGG
                     GQVSTRPPSTRSSTRSLAGPAARPALRPAVAPAPSSGS"
BASE COUNT          296 a          742 c          787 g          262 t
ORIGIN      
        1 cgacatacga ggtcaggtgg accgcgcgcc acgctccgac gctgagccgg ccacgcagca
       61 ggctcgtgac aacgacggcg agcgtgaggt cgagggcgag ggtcccgagg gagaggaaag
      121 ccgccttgta tgtcgcgccg aagggcagca gcgcctgcca ccaccggatg tcgacgtact
      181 cgtcgacgac ggccgtggcc acgtggagga cgagcaggat cactgagagc aggccgaggc
      241 tgcgatgcag cccctgcgtg aggaaccgtg gccaccagcg gggcgtcgcc cgcgacgtcg
      301 acaggacccc gccgacggtc gtcagggtga acaggacaag caggaccagg ccggtcccgc
      361 ggttgaggta ccacatcatg ggcgaggtca tcggatctcc agtggcaggg acgcggtctg
      421 agaggactcc cgcgagtccg gccagcctgg ggtgacatgg acggtcccgt cggtcccgac
      481 gagccgggcg gcgaccgccc gtgcctcgag ccaggccagc gcgtcgtctc ccccgacgag
      541 cgccgcggtg gtcgccgtgt tggcggcgct gcacgtgtgg cccgcagcgg taatggtgcg
      601 ccaggggcct gcggccgggg tcccggtgcg ggggtcgaag acgtggtgcc agtgccgtcc
      661 gccgcggacc cagcggcgcg cccgcacggt ggacgtggcc agcccaccgg accggagcag
      721 gacgaccggt gggtccgtgc caggatctcg gccagtggct cggccggtga gatccgctgc
      781 cgtctcggcg atctcgatcg gccagtcccg atcgtcgccg gagggcgcct cgacgcgcac
      841 gtcgccgccg atgctcacga ccgtggggat cctaaggcgc tcgacaaccg tggccgcgac
      901 gaggtcggcc gcgtaggcct tgccggtggc gccgaggtcc agggcgacac ccgttgggac
      961 gcacaggcgc ccgccggggt cgacctcgac gtcgcgccag gcctgaggcc ggctcatcgt
     1021 gggcagggca gcgggttcgt cggtcgcgcc gccgagcaga gcgaaggtcc ggtcatagcc
     1081 cgcgtcgcgc agcaggagcc ccagggtggg gtcgaccagc ccaccggtct cgcgtgccgc
     1141 ttcgagggcg accacgacgg cgcccgcgag gacgggcgac acgtcgaccc accggccggc
     1201 ctgggcgttg gccctgacca ggtccgagtc gtcgcggaac cggctgcagg acaggtcgac
     1261 ctcggcgagc acgtccctcg ccagagcctg cgccgagtcg ctggctgtgg ggtcaccgac
     1321 ggcaaggtag acgtaggtgc cgagggccgc ccagcgggac acacccggta ccgtggcgac
     1381 gacgtcggtc ctgctgatgt caggaccccg aggacggcgc gggggcgaca gccggccgca
     1441 gggcgggtcg cgccgccggg ccagcgaggc tccgggtgct ggaccgggtg gacggcggac
     1501 gggtcgagac ctggccgcca ccgacagctg ccgagcccga tcgggtgctc ccgtccacga
     1561 ccgtggtcgt cacgacggtt ctggtcggcc gcttcgcgag gagcgggaca gccgcctggg
     1621 ccgcggtctg cgcggcggcc tcggcggcgc ccttggtctg cgcctgcgct gccctcgccg
     1681 ccgccgcgtc ctcggtcgtc ttggccgcca cggccgtggc agcgccgacg gcggcgacgg
     1741 ccgccgcagt cgccaacccg gtgagggccg tcagaacatg acgaaaccgg ttgcgctgca
     1801 agggggtcag agcggtcttc atgggcgttt cccgtcagtg agcggctggt acggccctca
     1861 cgatgcccgc ccccgcggca aggcgacctc agcgcgacgt taagtaacca tcaggtcctg
     1921 cggtgagctt cctgggtctt gagtgcgtct gggagctcgg gttgcctcgc tgccctctcc
     1981 tgacaccctg tcctgacacc ctgtccctgc cacctgcccg gcacaccgag gccgacccgg
     2041 ccgcccagca ccggccacaa ccacctcggc aaccccgggc gcgacac
//
LOCUS       DATAIX010000433         1915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_173003, whole genome shotgun sequence.
ACCESSION   DATAIX010000433 DATAIX010000000
VERSION     DATAIX010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173003"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1016)
                     /locus_tag="VHM65_06540"
     CDS             complement(159..1016)
                     /locus_tag="VHM65_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2705398.1"
                     /translation="MALDTSLVTELKGLDALETHEEHAPLGARIWAGMWPKLAAVGLF
                     LGLWEGIVLLGLKPEYVLPGPAAALGEFAHQLTESSFWNAVAVTFTRGVVGFVIAAAV
                     GLVLGIVVAKSRILRTAIGSLITGLQTMPSIAWFPLAILLFQLTEQAILFVILIGAVP
                     SIANGVIGGVDYVPTLLVRAGRNIGARGLALYRHVILPAALPSIVSGLKQGWAFAWRS
                     LLAGELIVAIANRPSLGQFLSNSRELGDPPYMIALMIAILLIGVLVDAVFSGIDSGIR
                     RRRGVLASR"
     gene            complement(1006..1836)
                     /locus_tag="VHM65_06545"
     CDS             complement(1006..1836)
                     /locus_tag="VHM65_06545"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2705399.1"
                     /translation="MTIMATRRGGTSAALTVVPAGPGADATGAGPDHAVVLDRVTKRY
                     GSAQTSLALEGISLSVRRGEFVCLVGASGCGKSTLLNLVSGLDKPTSGRVSAEARPAF
                     MFQEHALLPWLSALDNVAMPMRLAGVDKPERTRRATEFLAAVHLADAADRRPHELSGG
                     MRQRVALARTLATESPVLLMDEPFGALDAMTRDLLHDELESIWRQRGLTVLFVTHNVR
                     EAVRLADRVVLLSSRPGRVVQEYPIEIGRPRRYDNHEVSHAAAEITAALRQEVARHGA
                     "
BASE COUNT          311 a          654 c          700 g          250 t
ORIGIN      
        1 gggtgctccc ccgccctccg cgtcatcctc gcgcaggata gtggcccctc gcggcgcaaa
       61 tatccgcacc gaggagccac tgaccggcgc gtggatggag gcaggccggc aggggaggca
      121 gggagggcgg ccctggcggc ggccgcccct cccgcggctc acctggacgc gagcacaccc
      181 cgccgacggc ggatccccga gtcgatgccc gagaagacgg cgtcgacgag caccccgatg
      241 agcaggatgg cgatcatcaa ggcgatcatg taaggcgggt cgcccagctc gcggctgttg
      301 gacaggaact gcccgaggct cggccggttg gcgatcgcca cgatgagctc tcccgcgagc
      361 agcgaccgcc acgcgaaggc ccatccctgc ttgagcccag acacgatgct cggcagagcc
      421 gccggcaaga tgacgtggcg gtagagggcc aggcccctgg ccccgatgtt gcgccccgcg
      481 cggacgagca gggtcggcac atagtcgacc ccgccgatga cgccgttggc gatcgaggga
      541 accgctccga tgaggatgac gaagaggatg gcctgctcgg tcagctggaa cagcaggatg
      601 gcgagcggga accaggcgat cgagggcatg gtctgcagcc ccgtgatgag cgaaccgatc
      661 gccgtgcgca ggatccggga cttggccacg acgatcccga ggacgagacc gaccgcagcg
      721 gcgatgacga acccgaccac ccctcgggtg aaggtcactg ccaccgcgtt ccagaaggag
      781 ctttcggtga gctggtgggc gaactccccc agcgccgccg cgggcccggg caggacgtac
      841 tccggcttga gtcccagcag gacgatgccc tcccacagcc cgaggaagag cccgaccgcg
      901 gccagcttgg gccacatgcc ggcccatatg cgggccccca ggggggcgtg ctcctcgtgg
      961 gtctcgagcg cgtcgaggcc cttgagctcg gtgacgagac tggtgtcaag cgccatgacg
     1021 agccacctcc tgacgcaggg cagccgtgat ctcggctgcc gcgtgggaga cctcgtggtt
     1081 gtcatagcgc cgaggtcggc cgatctctat ggggtactcc tggacgacgc ggccggggcg
     1141 gctcgacagc aggacgaccc ggtcggcgag ccggacggcc tcgcggacgt tgtgggtcac
     1201 gaagaggacg gtgaggccgc gctggcgcca gatcgactcc agctcgtcgt ggagcaggtc
     1261 ccgggtcatc gcgtcgagcg ccccgaaggg ctcgtccatg agcaggaccg gtgactccgt
     1321 cgccagggtc ctggccaggg ccacccgctg gcgcatcccg ccggacagct catgcggtcg
     1381 gcgatcggcc gcgtcagcga ggtgcacggc ggcgaggaac tccgtagcgc gccgagtccg
     1441 ttcgggcttg tcgacgcccg cgagccgcat cggcatagcg acgttgtcca gggccgagag
     1501 ccacggcagg agcgcgtgct cctggaacat gaacgccggt ctggcttcgg cgctgacccg
     1561 tcccgaggtg ggcttgtcca gcccactgac gaggttgagc agcgtcgact tcccgcaccc
     1621 cgatgctccg acgaggcaga cgaactcccc gcgccggacg gacagggaga ttccctccag
     1681 ggcgagcgag gtctgggcgc tcccgtagcg cttggtgacc cggtcgagca cgaccgcgtg
     1741 gtcgggccca gcgcccgtgg cgtcggcgcc aggccccgcc ggcaccacgg tgagcgccgc
     1801 ggaagtcccg ccgcggcggg ttgccatgat cgtcatgacg ccaccgccgg aagcccgcgg
     1861 gccttgagga cggtgttgag gatcgacagg tcggtgaggc cgtcgagctt cggct
//
LOCUS       DATAIX010000434         2176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_23557, whole genome shotgun sequence.
ACCESSION   DATAIX010000434 DATAIX010000000
VERSION     DATAIX010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23557"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1634
                     /locus_tag="VHM65_06550"
     CDS             126..1634
                     /locus_tag="VHM65_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEX2705400.1"
                     /translation="MSSERQAKTDNRRRPTTDELRAFIARGWAPRDAARPERAAVAPY
                     AAARRERVSAAFPGERLVIPAGGLTVRSNDTDYLFRPHSAFAHLTGLGADREPDAVLV
                     LEPRPDGGHEAVLFMRPRADRDSDEFFADTRYGEFWVGARPTIADLEAELGITARHLG
                     EFAGAIAKDIGVVGIRVVRDADRDVAEQVDAARQESATETSGAQAETAREADLALAAF
                     LSSARLVKDEWEIEQMRLAVAATHVAFEAVIAGLPEAVRGGRGERWVEGVFGLHARHQ
                     GNGIGYDSICAAGDHATTLHWIKNSGEVHEGVLILIDAGVEIDSLFTADITRTLPVGG
                     TFTEAQREIYRAVYDAQQAGLAAAKPGNRFSDIHAAAIQVIAERLHAWGLLPEGVSVQ
                     DSLDADHGQYHRRWMVHGTSHHLGLDVHDCALATREEYLDAELVPGMVLTVEPGLYFK
                     ADDLLAPERFRGTGVRIEDDVVITADGCENLSSAMPRTAEDVEAWIARVQGA"
     gene            complement(1663..>2176)
                     /locus_tag="VHM65_06555"
     CDS             complement(1663..>2176)
                     /locus_tag="VHM65_06555"
                     /inference="COORDINATES: protein motif:HMM:NF022629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEX2705401.1"
                     /translation="SLKYPMSIGVFDTYDAAQKAVDTLSDQQFPVQNVLIVGTDLKQM
                     ERVTGRLTRGRVALGGLLSGVWLGLFVGLVFSLFVTTTSGLVVILSTAVYGAVFGLVW
                     ALIGYALTGGHRDFTSVSQVVATRYEVLVEHKFAQQARELLVAAGLIPGYLPPPMQAP
                     SPAPPPADVS"
BASE COUNT          345 a          792 c          755 g          284 t
ORIGIN      
        1 tgtcccagac gatgcccgac gacgcctcgg ccgacgcagc acgcctcgcc gccccggaca
       61 tcaccggctg accccggggg cgacgggaga cccgagccac gcccggcaat ggcaccatgg
      121 tgtgcgtgag tagcgagcga caggccaaga ccgacaaccg ccggaggccg acgacggacg
      181 agctgcgggc cttcatcgcc cgcggctggg cgccccggga cgcggcccgg cccgagcgag
      241 ccgcggtcgc gccgtatgcc gccgcccgcc gcgagcgcgt gtccgccgcg ttcccgggcg
      301 agcggctcgt cataccggct gggggtctga cggtccgcag caacgacacc gactatctct
      361 tccgcccgca cagcgcgttc gcccacctca ccggcctcgg ggcagaccgc gagcctgacg
      421 cggtcctcgt cctcgaaccc cggcccgacg gtggtcatga ggcggtcctg ttcatgcggc
      481 cccgcgccga ccgcgactcg gacgagttct tcgcagacac ccgctatggc gagttctggg
      541 tcggagcccg gccgaccatc gccgacctcg aagccgagct ggggatcacc gctcggcacc
      601 tcggggagtt cgccggagct atcgccaaag acattggggt tgttgggatt cgggtcgtcc
      661 gtgacgccga ccgcgacgtc gccgagcagg tcgatgccgc tcgccaggag tcggccaccg
      721 agacctcagg cgcgcaggcc gagacggcac gggaggccga cctggcgctc gcggccttcc
      781 tctccagcgc gcgactggtc aaggacgagt gggagatcga gcagatgcgc ctggccgtgg
      841 ccgcgaccca cgtcgccttc gaggccgtca tcgccggcct ccccgaggcg gtccgtggcg
      901 gccgcggcga gcgctgggtc gagggcgtgt tcgggctcca cgcgcgccac cagggcaacg
      961 ggatcggcta cgactcgatc tgcgcggccg gtgaccacgc cacaaccctg cactggatca
     1021 agaacagcgg cgaggtccac gaaggcgtgc tgatcctcat cgacgcgggt gtcgagatcg
     1081 actcgctgtt caccgccgac atcacgcgca ccctcccggt cggtggcacc ttcaccgagg
     1141 cgcagcgcga gatctaccgc gcggtgtatg acgcgcagca ggccggcctc gccgcggcca
     1201 agcccggcaa ccgcttctcg gacatccacg ctgcagccat ccaggtcatc gccgagcgcc
     1261 tccatgcctg gggcctgctg cccgagggcg tgtccgtgca ggactccctg gacgccgacc
     1321 acggccagta ccaccggcgc tggatggtcc acggcacgag ccaccacctc ggcctggacg
     1381 tgcacgactg cgcgctggcc acccgcgagg agtacctcga cgccgagctc gtgcccggca
     1441 tggtcctcac ggtcgagccg gggctctact tcaaggccga cgacctgctc gcgccggaac
     1501 gcttccgggg caccggagtc cggatcgagg acgatgtcgt catcaccgcg gacggctgcg
     1561 agaacctctc ctcggccatg ccgcgcacgg ccgaggatgt cgaggcgtgg atcgcccggg
     1621 tgcagggcgc ctgacccccc gggtcacctg cccttctggc agtcagctca cgtccgcggg
     1681 cggcggtgcc ggcgaaggcg cctgcatcgg aggtgggagg tagcccggga tcagcccggc
     1741 cgcgaccagg agctcacggg cctgctgggc gaacttgtgc tcgacgagca cctcgtagcg
     1801 ggtggcgacg acctggctca ccgacgtgaa gtcccggtgg ccaccggtca gcgcatagcc
     1861 gatgagcgcc cagacgaggc cgaagaccgc gccatacacg gccgtggaga ggatgacgac
     1921 gagcccggac gtcgtcgtca cgaagaggga gaagaccagc cccacgaaca gacccagcca
     1981 cacgccggag agcaggccac cgagggccac ccggccgcgg gtcagccgac cggttacgcg
     2041 ctccatctgc ttgaggtctg tcccgacgat gaggacgttc tggaccggga actgttgatc
     2101 cgagagggtg tcgacggcct tctgggcggc gtcgtaggtg tcgaagaccc cgatcgacat
     2161 cgggtacttc agcgac
//
LOCUS       DATAIX010000435         2076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_23566, whole genome shotgun sequence.
ACCESSION   DATAIX010000435 DATAIX010000000
VERSION     DATAIX010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23566"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..427)
                     /locus_tag="VHM65_06560"
     CDS             complement(113..427)
                     /locus_tag="VHM65_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HEX2705402.1"
                     /translation="MPTTTMAGRPIDVNDEGFLTDPAQWDEALADTLAGNIGLTLTEA
                     HWKILHFLREDFATQHETATLRRVSTQAGVPVKELFVLFPGKPAKKMSYVAGLPKPKG
                     CV"
     gene            complement(501..1736)
                     /locus_tag="VHM65_06565"
     CDS             complement(501..1736)
                     /locus_tag="VHM65_06565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX2705403.1"
                     /translation="MKTLLVLGGGTAGTMVANKLRARLGADEWTVTVVDRDDIHDYQP
                     GYLFIPFGINTPAQIRRTKHKFFAHGVQVVMGEIERVDPDGRAVLLADGRSLAYDYLV
                     IATGTTPRPDQTPGMLGPEWHKSVGEFYTYDGAVALAEQTKTFTGGRRVMHITEMPIK
                     CPVAPLEFTFLADDWLRQRGLREKTELVFVTPLDGAFTKPVASRELGSALARRGVIVE
                     TDFMIERVDSERKVIVSYDEREIAYDQLVTVPLNMGADFVARSGLGDELNYVPVDKHT
                     MRSVAHDTIFALGDAGNLPTSKAGSVAHFSVEVFVENFLQLAAGQPMTHSFDGHANCF
                     IESGGGKGMLLDFNYDTEPLTGTFPLPKVGPMSLLKESRANHLGKLAFRWVYWNVLLP
                     GRPLGLPADMSMAGKNVES"
BASE COUNT          315 a          635 c          779 g          347 t
ORIGIN      
        1 gagcagatga tggcgagctt gcgaccgctg ggcgtttcgt cgtcgaacga cgggacgatc
       61 gtcggctctg gggcctgggg ggcctcgggg ctctcggtgg cgttggtggg catcacacac
      121 accccttggg cttgggcagg ccggcgacat acgacatctt cttggcgggc ttgcccggga
      181 agaggacgaa gagctccttg accgggaccc cggcctgggt gctcacccgc cgcagcgtgg
      241 cggtctcgtg ctgggtggcg aagtcctcgc gcaggaagtg caggatcttc cagtgggcct
      301 cggtcagggt gagaccgatg ttgcccgcga gggtgtcggc cagcgcctcg tcccattggg
      361 cggggtcggt aaggaagccc tcgtcgttga cgtcgatcgg ccgaccggcc atcgtggtgg
      421 tgggcatgat gtactgctgc tccttctggg cgtgaaatgc cctgtggcga ttcaggattg
      481 gcgattcagg attgggtggt tcaggactcg acgttcttgc cagccatcga catgtccgcg
      541 ggcagcccca gcgggcgacc ggggagcagc acgttccagt agacccagcg gaaggcgagc
      601 ttgcccaggt ggttggcccg gctctccttg agcaggctca tcgggccgac cttgggaagg
      661 gggaaggtgc cggtcagcgg ctcggtgtcg tagttgaagt cgagcagcat ccccttgccg
      721 cccccggact cgatgaagca gttggcgtgg ccgtcgaagg agtgggtcat cggctgaccc
      781 gcggcgagct ggaggaagtt ctcgacgaag acctcgaccg agaagtgggc gacggacccg
      841 gccttgctcg tcgggaggtt cccggcgtcg ccgagggcga agatcgtgtc gtgcgcgacc
      901 gaccgcatcg tgtgcttgtc gacggggacg tagttgagct cgtcaccgag gccggagcgg
      961 gcgacgaagt cggcgcccat gttgaggggg accgtgacga gctggtcgta ggcgatctcg
     1021 cgctcgtcgt aggagacgat gaccttgcgc tcggagtcga cccgctcgat catgaagtcg
     1081 gtctcgacga tgacgccccg tctggccagg gccgacccga gctcgcggct ggcgacgggt
     1141 ttggtgaacg cgccgtcgag cggggtcacg aacacgagct cggtcttctc gcgtaggccg
     1201 cgctgacgca gccagtcgtc ggcgaggaag gtgaactcca gcggcgcaac cgggcacttg
     1261 atcggcatct cggtgatgtg catgacccgg cgccccccgg tgaaggtctt cgtctgctcg
     1321 gccagggcaa ccgctccgtc atacgtgtag aactcaccga cgctcttgtg ccactcgggg
     1381 ccgagcatcc ccggggtctg gtcgggccgt ggggtcgtcc ccgtggcgat gacgaggtag
     1441 tcgtaggcga ggctgcgccc gtcggcgagc aggacggcgc gcccatcggg gtcgacccgc
     1501 tcgatctcac ccatgacgac ctggaccccg tgggcgaaga acttgtgctt cgtccggcgg
     1561 atctgcgccg gggtgttgat gccgaacggg atgaacaggt agccgggctg gtagtcgtgg
     1621 atgtcgtcgc ggtcgacgac cgtcacggtc cactcgtcgg cgccgagccg ggcgcggagc
     1681 ttgttggcaa ccatcgtgcc ggcggtgcct ccgccgagca caaggagggt cttcatggtg
     1741 caggcacgtc cgttcacgag aggctggtca cctcaaacat acccccatgg gtatgacgcg
     1801 cggcggggac caacggccca tcggcactgt gttctcgggc acacggtgtt ctcgggcaca
     1861 cggtgttctc gggcacacgg gcgagggcgg ggccccggct caggcctgaa gcgcggcctc
     1921 gaaggtggcg cgcagccggg cgctgaacag gacgaagcgg accagctcga ggcggtcgag
     1981 ccgggggaac ccgcggacgg ccgccacggc gatgcgcgcc acgtctgcgg cgtcccaccc
     2041 gtaggcgccg gcactcacgg cggggaaggc caccga
//
LOCUS       DATAIX010000436         1526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_53894, whole genome shotgun sequence.
ACCESSION   DATAIX010000436 DATAIX010000000
VERSION     DATAIX010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53894"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..>1526)
                     /locus_tag="VHM65_06570"
     CDS             complement(39..>1526)
                     /locus_tag="VHM65_06570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX2705404.1"
                     /translation="EKVATDGVVADGHSALDTSIITGESLPVDVAPGDRVTGGTVNTH
                     GRLVVTARSVGADTVLAAMTRLVEAAQTGKAEVQRLADRVSAVFVPVVLGIAALTLLG
                     WLVTRHPAEQALSVAISVLIIACPCALGLATPTALLVGTGRGAQLGVLIKGPQVLEDT
                     RRVDTVVLDKTGTVTSGVLRLDQVRTTGELSEADALALAAAVEAGSEHPIARAVVRAA
                     QERGMALAGASGFIAMPGLGARAVVGGVEVSVGKAGLFEAVPQRLHPTTEDTGTTVYA
                     GWSGAARAALTLSDTVRVSSPAAVARLRRLGLTPYLLTGDHAAAARWVATRVGIDEAH
                     VIADVLPRDKYDVVRRLQAQGRVVAMVGDGINDAAALVQADLGVAMGSGTDVAMDAAD
                     IVLMRGDLDAAADAIALSRQTLRVIRQNLAWAFGYNAAAIPLAVLGLLNPMIAGATMA
                     MSSVLVVTNSLRLRRFRARGDGVGPHDEDGLGPEPAGASGPQMVT"
BASE COUNT          204 a          559 c          569 g          194 t
ORIGIN      
        1 gcctgcccct gcgccctggg cctggccacc ccgaccggtc acgtcaccat ctgaggtcca
       61 gacgcgccgg ccggctcagg cccaaggccg tcctcatcgt gcggcccaac cccgtcaccg
      121 cgggcgcgga accggcgcag ccgcaggctg ttcgtcacca cgagcacgct cgacatcgcc
      181 atcgtcgcgc ccgcgatcat cgggttgagc aggccgagca cggcaagggg gatcgccgcc
      241 gcgttgtagc cgaaggccca cgcgaggttc tggcggatca cccgcaacgt ctggcgtgac
      301 aaggcgatcg cgtcggccgc cgcgtcgaga tcgccgcgca tcaggacgat gtcggccgcg
      361 tccatggcga cgtcggtccc cgaccccatc gcgacgccga ggtcggcctg gacgagggct
      421 gcggcatcgt tgatgccgtc gccgaccatc gcgacgaccc gcccctgcgc ctgcagccgc
      481 cgcaccacgt cgtacttgtc ccgcggcagg acgtcggcga tgacgtgtgc ctcgtcgatc
      541 cccacccggg tggcgaccca tcgcgcggca gccgcgtggt caccggtgag caggtatggc
      601 gtgaggccga gccggcgcag ccgcgccacc gccgcgggtg aggagacccg tacggtgtcg
      661 ctcagcgtga gggccgctcg cgcggcgccg gaccacccgg catacacggt ggtccccgtg
      721 tcttcggtgg tggggtgcag tcgctgcggg acggcctcga agaggcccgc cttgccgacg
      781 ctcacctcga ccccgccaac gacggcgcgg gccccgagcc cgggcatcgc gatgaaccca
      841 ctggcgccgg ccagcgccat tccccgctcc tgggcggccc ggacgacggc ccgggcgatg
      901 ggatgctcgc tgccggcttc gacggcggcg gcgagcgcca gggcatcggc ctcggagagc
      961 tcgcccgtcg tccgcacctg gtcgaggcgc aggaccccgc tggtcacggt gccggtcttg
     1021 tccaggacga ccgtgtcgac ccggcgggtg tcctcgagga cctgcggtcc cttgatgagg
     1081 acgccgagct gcgcgccgcg gccggtcccg acgagcagtg cggtcggggt ggccaggccc
     1141 agggcgcagg ggcaggcgat gatgaggacg ctgatcgcca ccgacagcgc ctgctcggcc
     1201 gggtgccggg tgacgagcca gccgagcagg gtgagcgcgg caatgccgag gacgactggg
     1261 acgaagaccg cgctgacccg gtcggcgagc cgctggacct cggccttgcc ggtctgggcc
     1321 gcctcgacga gccgggtcat ggcggccagg accgtgtcgg ccccgaccga gcgggcggtg
     1381 acgacgagac gcccgtgggt gttgacggtg ccaccggtga cccggtcgcc aggagcgacg
     1441 tcgaccggga gggactcgcc ggtgatgatc gaggtgtcga gcgcgctgtg gccgtcggcg
     1501 accacgccgt cggtggcgac cttctc
//
LOCUS       DATAIX010000437         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_53931, whole genome shotgun sequence.
ACCESSION   DATAIX010000437 DATAIX010000000
VERSION     DATAIX010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53931"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1575)
                     /locus_tag="VHM65_06575"
     CDS             complement(<1..1575)
                     /locus_tag="VHM65_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2705405.1"
                     /translation="MTLPPPSGQPVDLAAPGEAAIRCRGLVKRYGQTVAVAGLDLDFP
                     KGSFFGLVGPNGAGKTTTLSMVTGLLRPDAGSVRVLGKDVWTDPVAAKRMLGVLPDGM
                     RLFDRLTGLQLVTYAGLLRGMDRAVVVGRAHELLAALGLGGDASTLVVDYSAGMTKKV
                     ALACALVHAPRVLVLDEPFEGVDPVSASTIKEILDSYVASGRDGRAVQPRHGAGRAPV
                     LARRGYRGGPGPRCRLGRRSASRDEPAGPVRRPRRGPHPHRRAVVVAHLVRLKLLLLR
                     NGLKRRPSQLIGLGVGVLYGGFAVVVACAGLVALRFAPDPRLGGALVTVLGSVLVLAW
                     GVVPLFAFGTDPTLDPRRFATFAISSRQLALGLAASGLVGLPALAWTRATAAMVENPR
                     GVTAISASEKVAGLGMFGRLPGTPVGAVAARSLTMWRRDPRYQVSALALLLVPLVLLV
                     ASTASGASGTAFLGMPVLTRRFPVSGPGVTVRWRAEGLPGSRVAGVGRWRGSLLSRGA
                     LGCCSRLLGRRTPRRCG"
     gene            complement(1572..>2138)
                     /locus_tag="VHM65_06580"
     CDS             complement(1572..>2138)
                     /locus_tag="VHM65_06580"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2705406.1"
                     /translation="GFEDLRDLDAVVVTHQHADHLDLARLGDLVWANPGVAVYADPQS
                     QQILAAAGIDTVVTRSGAQVLVGEVTLTGVGTEHAFNHEWMPTVANVGVVLRATGEPS
                     IYHPGDAYDGEAGPVDVLAVPINAPWTAVRDSIAFVRRVAPGQVVPIHDALLSPQGRV
                     LYLGHIGANGGGAAVTDLVGRGAVTVGG"
BASE COUNT          364 a          776 c          755 g          243 t
ORIGIN      
        1 gccacaccgg cgcggcgtac gacgacccag gagccgacta caacaaccga gtgcaccccg
       61 agagagcaaa gaaccacgcc atcgcccaac tccagcgact cggcttccag gtaacccttc
      121 agcccgccac ctgacggtca ccccaggtcc tgacacgggg aatcttcgtg tcaggacagg
      181 cataccgagg aaggcggtcc ccgatgcccc tgatgcggtg ctggccacca gcaggaccag
      241 gggtaccagg agcagcgcta gcgcgctgac ctggtacctc gggtcgcggc gccacatcgt
      301 cagcgaccgg gcggcgaccg ccccgaccgg cgttcccggg aggcgcccga acatccccag
      361 gccggcgacc ttctcggacg cggagatggc ggtcacgccc cgcgggttct ccaccatggc
      421 agcggtggcg cgcgtccagg cgagcgccgg cagcccgacg aggccgctcg cggcaagccc
      481 gagcgcgagc tgacggctcg agatggcgaa ggtcgcgaag cgcctcgggt ccagggtcgg
      541 gtcggtgccg aaggcgaaca aggggacgac cccccaggcc aggacgagga ccgacccgag
      601 caccgtcacc agagctcccc cgagtcgtgg gtcgggagcg aaccgcagcg cgacgagacc
      661 tgcacaggcc acgaccacgg cgaacccgcc gtagaggacg ccgacgccga gtccgatgag
      721 ctggctgggc cgccgtttca ggccgttgcg cagcaggagg agcttcagtc ggacgaggtg
      781 cgcaaccacg acagcccttc gatgtgggtg cggcccccga cgaggtcgac gaaccggtcc
      841 tgcaggctca tcccggctcg cactgcgccg accgagccga cagcgcggac ctggcccgcc
      901 gcgataaccg cgacgtgcga gcacaggcgc tcgaccagct ccatgacgtg gctggacagc
      961 acgaccgtcc cgcccggagg cgacgtagga gtcgaggatc tccttgatcg tcgacgccga
     1021 caccgggtcg acgccctcga acggctcgtc gaggacgagc acccgcgggg cgtgcaccag
     1081 ggcacacgcg agggcgacct tcttcgtcat accggcggag tagtcgacga cgagcgtgct
     1141 cgcatccccg ccgagcccga gcgccgcgag cagctcgtgg gcgcgcccga cgacgaccgc
     1201 ccggtccatc ccccggagca ggcccgcgta ggtcaccagc tgcagcccgg tgagccggtc
     1261 gaaaaggcgc atgccgtccg gcagcacccc gagcatccgc ttggccgcga ccgggtcggt
     1321 ccagacgtcc ttgccgagca cgcgcaccga gccggcatcg ggacgcagca ggccggtgac
     1381 catagacagc gtcgtcgtct tgcctgcccc gttgggaccg acgagcccga agaacgaccc
     1441 cttggggaag tcgaggtcca gccctgccac ggcgacggtc tggccgtagc gcttgaccag
     1501 gccccggcag cggatcgccg cctcgcccgg ggcagcgagg tcgacgggct gacccgaggg
     1561 cggcggcagg gtcatccgcc gacggtcacg gcgccccggc cgacgaggtc ggtgaccgca
     1621 gcgccaccgc cgttggcgcc gatgtgaccg aggtagagga ctcggccctg aggcgacagc
     1681 aaggcgtcgt ggatcggcac gacctgcccg ggcgcgaccc gccggacgaa ggcaatcgag
     1741 tcccggaccg cggtccacgg ggcgttgatg ggcacggcga ggacgtccac cgggccggcc
     1801 tccccgtcgt aggcgtcacc cggatggtag atgctgggtt caccagtcgc ccgcagcacc
     1861 accccgacgt tggcgaccgt cggcatccac tcgtggttga aggcgtgctc ggtcccgacc
     1921 ccggtcagcg tgacctcacc gaccagaacc tgcgcacccg accgggtcac gacggtgtcg
     1981 atccccgccg cggcgaggat ctgctggctc tgcgggtcgg cgtagacggc cacgccgggg
     2041 ttggcccaga cgaggtcgcc gagccgagcc aggtcgaggt ggtcggcatg ctggtgggtg
     2101 acgacgacgg catcgaggtc gcgcaggtcc tcgaagcc
//
LOCUS       DATAIX010000438         2426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_248836, whole genome shotgun sequence.
ACCESSION   DATAIX010000438 DATAIX010000000
VERSION     DATAIX010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248836"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VHM65_06585"
     CDS             <1..203
                     /locus_tag="VHM65_06585"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX2705407.1"
                     /translation="DIFLDDVTVSRRHAVFTRTGTGYSIRDVGSLNGTYVNRTLADDV
                     ALNTGDEVQIGKFRLMYYAAGA"
     gene            242..955
                     /locus_tag="VHM65_06590"
     CDS             242..955
                     /locus_tag="VHM65_06590"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2705408.1"
                     /translation="MPSSRRAQAMRIGAVIRALTPDFPDVTVSKIRFLEAEGLVTPER
                     TPSGYRLFSAADLERLRYILAAQRDRFWPLRVIRESLDAIDRGLSAQPPDAGSGRPSV
                     PATLADPALPTPASLTAPARLRLTEAELREACGLEPQMLTALTSFGLLHADESGHYDD
                     HQLTVARAAGALAAYGVEARHLRAFRSAADREIGLVRQIVTPARSAGGKHAPADPTAE
                     VLAHCIALHAALVAADLIR"
     gene            1000..1479
                     /locus_tag="VHM65_06595"
     CDS             1000..1479
                     /locus_tag="VHM65_06595"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX2705409.1"
                     /translation="MREVEVLGVRVEMPTNQPIVLLREQDGDRYLAIWIGAAEATAIA
                     YAQQGVVPPRPLTHDLFKVTLDALAHQVTQIRITELRDKVFYAVLVIDGTTEISARPS
                     DAIAIALRTGTGVVVADEVLDEAGVVLPADEDDEVEKFREFLDHVSAEDFVEPPPTE"
     gene            1796..2347
                     /locus_tag="VHM65_06600"
     CDS             1796..2347
                     /locus_tag="VHM65_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2705410.1"
                     /translation="MNATDGSHEGHRAVPLAVQGLLFTDDLPVLSEDVGYRGPTACRA
                     AGITYRQLDYWARTGLVDPSVRSATGSGTQRLYGFRDILVLKVVKRLLDTGVSLQQIR
                     VAISHLRERGVDDLAQITLMSDGASVYECTSADEVIDLVQGGQGSSASRSAACGVRSR
                     APSPTCPASGPRTRRRRRTPRTS"
BASE COUNT          358 a          886 c          830 g          352 t
ORIGIN      
        1 gcgacatctt cctcgacgac gtgaccgtgt cgcgccggca cgccgtgttc acccggaccg
       61 gcacgggcta ctcgatccgc gacgtcggct ccctcaacgg cacctacgtc aaccgcaccc
      121 tggccgacga cgtcgccctg aacaccggcg acgaggtcca gatcggcaag ttccggctga
      181 tgtactacgc cgcgggcgcg tgagccggcc ggcgtccccg agtggaggac ccgaccaggg
      241 tgtgccgagc tcgcggcgcg cccaggcgat gaggatcggg gcggtcatcc gtgccctcac
      301 ccccgacttc cctgacgtca cggtgtccaa gatccggttc ctggaggccg aggggctggt
      361 cacccccgaa cgcacgccct cgggctaccg cctgttcagc gcggccgacc tcgagcggct
      421 cagatacatc ctggccgccc aacgggatcg gttctggccc ctgcgcgtca tccgtgagtc
      481 gctcgacgcg atcgaccgcg gcctgagcgc gcagccgccc gacgccggat cggggcgccc
      541 gagcgtgccg gccacgctcg ccgacccggc gctgccgacg cccgcctcgc tgaccgctcc
      601 ggcccgcttg cgcctgaccg aggccgaact gcgcgaggcc tgcgggctcg agccgcagat
      661 gctcaccgcg ctgacctcgt tcgggctgct gcacgccgac gagtccggtc actacgacga
      721 ccaccagctg accgtggctc gcgcggctgg ggcactcgcg gcctacggcg tggaagcccg
      781 tcacctgcgc gccttccgct cggcggcgga ccgtgagatc gggctggtcc gccagatcgt
      841 gacacccgcg cgctcggctg gtggcaagca cgcgccggcc gacccgaccg ccgaggtcct
      901 ggcgcactgc atcgcgctcc atgcggcgct cgttgccgcc gacctcatcc gctaggccgg
      961 tgcgggacga ggcacggcgg tacggggtac ggtggtcttg tgcgcgaggt cgaggtgctc
     1021 ggagtccggg tcgagatgcc cacgaaccag cccatcgtgc tcctgcggga gcaggacggt
     1081 gaccggtacc tcgcgatctg gatcggggcc gccgaggcca ccgccatcgc ctacgcccag
     1141 cagggtgtcg tcccccctcg gccgctcacc cacgacctgt tcaaggtgac cctcgacgcg
     1201 ctcgcgcacc aggtgaccca gatccggatc accgagctgc gggacaaggt cttctatgcc
     1261 gtccttgtca tcgacgggac gaccgagatc agcgcccggc cctcggacgc gatcgcgatc
     1321 gccctgcgca ccgggacggg cgtcgtcgtc gccgacgagg tgctcgacga ggccggagtc
     1381 gtcctgcccg cggacgagga cgacgaggtc gagaagtttc gcgagttcct cgaccacgtg
     1441 tcggcggagg acttcgtcga gccgccgccc accgaatagc ggtatgcccg ggccgccggc
     1501 tcgctctgcc gtcctcgctc tgccgtcgag gacccgcgtc agggcatcca gccgacgctt
     1561 cgttccagcc gacccctacc tcaccgtaac gtagacgttg agagttgtgt gaccggagtg
     1621 gcgaaagaca cgcatgggaa aagagtgccc cggtgattga cggtcggctt cggggggcct
     1681 accgtcagaa gtcagaacaa cgcaagtgta gttacctggg ctggagttgg cctcagcacg
     1741 gcatgacccg tcccgccgcc ggcgctggga cgcaggtagg gacgaggagg cagccgtgaa
     1801 cgccacggac ggatcgcacg agggccaccg ggccgtcccg ctggcggtgc agggcctgct
     1861 cttcaccgac gacctgcccg tgctgagcga ggatgtgggc tatcgcggcc cgaccgcctg
     1921 ccgcgcggcc ggcatcacct accgtcagct cgactactgg gcgcgcaccg ggctggtcga
     1981 cccgtccgtg cgcagcgcga ccggttcggg cacgcagcgt ctctacgggt tccgcgacat
     2041 cctggtgctc aaggtggtca agcgactgct cgacaccggc gtgtccctgc agcagatccg
     2101 cgtcgcgatc agccacctgc gcgagcgggg cgtcgacgac ctggcccaga tcaccttgat
     2161 gagtgacggc gcctcggtct acgagtgcac ctctgccgat gaggtcatcg acctcgtcca
     2221 gggagggcag ggctcttcgg catcgcggtc ggccgcgtgt ggcgtgagat cgagggcacc
     2281 ctcgccgacc tgcccagcga gcgggccgag gacgagacgc cgtcggcgga ccccgcggac
     2341 gagctgagcc tgcgtcgcca cgcgcgccag ctggcctagt ccccgggcgc acgccgactt
     2401 ggtagattag gcgcgctaca tacacc
//
LOCUS       DATAIX010000439         1703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_129555, whole genome shotgun sequence.
ACCESSION   DATAIX010000439 DATAIX010000000
VERSION     DATAIX010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129555"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..730
                     /locus_tag="VHM65_06605"
     CDS             179..730
                     /locus_tag="VHM65_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705411.1"
                     /translation="MNEALWVLGRGTGTAALLLLTITAAIGVLTAGRFVSRDTPRFAV
                     AELHKRAALAAAGLLVVHLASLLFDSEAQLRWVDLVLPFLNARNPLFWGLGTLAVDLI
                     AVVTFTSLARKRIPHRWWRGIHWMSCALWPMAWLHSLGSGTDASTWWLRTVSVLTLSA
                     VVAAVTYRLRGPAPAVRHHVTAA"
     gene            870..>1703
                     /locus_tag="VHM65_06610"
     CDS             870..>1703
                     /locus_tag="VHM65_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2705412.1"
                     /translation="MAPELTLSHTVGDTSVPLLEETIGDNFDATVARFPDREALVDCL
                     PGRRWTYAELATDVDTFARALMAAGIVKGDRVGIWAPNCAEWAIVQYATAKIGAILVN
                     INPAYRTHELGYVLNQAGTRMLIAAPSFKTSNYAAMIEQVRPDVAALEQVVLIGTRSW
                     DALVAGAGEVSAAALAAVQAGLDRNDAINIQYTSGTTGFPKGATLSHRNILNNGYLVG
                     EICRYTEVDRICIPVPFYHCFGMVMGNLAATSHGACMVIPAPGFDPAATLQATQDERC
                     TS"
BASE COUNT          248 a          637 c          564 g          254 t
ORIGIN      
        1 cgtcagaggc gagcaggcgg tgcggaccct cctccggacc ggccatccgg cccggctgct
       61 cggcgccgac ctgtccctga cccatctggg cggctggcct gcggacggcg agctgacccg
      121 acataccggc tcaggcaggt ccgacggccg tcttgacgtc gagagctgag caggcgtcat
      181 gaacgaggca ctctgggtac tcggccgcgg caccggcacc gccgcactcc tcctgctcac
      241 catcaccgcc gcgatcgggg tgctgaccgc tgggcgcttc gtctcccggg acacgccgcg
      301 gttcgccgtc gccgagctgc acaagcgcgc cgcgctcgcc gccgccgggc ttctcgtcgt
      361 ccacctcgcg tcgctgctgt tcgacagcga ggcccagctg cgctgggtcg acctcgtcct
      421 gcccttcctc aacgcccgga acccgctgtt ctggggtctg ggcacgctgg cggtcgacct
      481 catcgcggtc gtcacgttca cctccctggc acgcaagcgc atcccgcacc ggtggtggcg
      541 ggggatccac tggatgtcct gtgccctctg gccgatggcc tggctgcact cgctcgggtc
      601 gggcacggac gcgagcacgt ggtggctgcg aaccgtgtcg gtcctgaccc tgtccgcggt
      661 tgtcgctgcg gtgacctacc ggctccgggg tccggccccg gccgtgcgcc accacgtcac
      721 cgcggcgtga cacactcggg ctgaaccagg gcttgcggtg ggcccggacg tggggtggac
      781 tactcgccat accgggtcgg ctgacggcct ggtccacgac tgcgcccccg agaccgacga
      841 cccgagacca acgacgcgaa ggcgacgaca tggcacccga gctcaccttg tcccacacgg
      901 tcggcgacac ctccgtgccg ctgctcgagg agacgatcgg cgacaacttc gacgcgacgg
      961 tcgcgcgatt cccggacaga gaggcgctcg tcgactgtct gcccggccga cggtggacgt
     1021 atgccgagct cgccaccgac gtcgacacgt tcgcccgggc cctgatggct gccggcatcg
     1081 tcaagggcga ccgtgtcggg atctgggcac cgaactgcgc cgagtgggcg atcgtccagt
     1141 acgcgacggc caagatcggc gcgatcctcg tcaacatcaa cccggcctac cggacccacg
     1201 agctcgggta cgtgctcaac caggcgggga cccggatgct catcgccgcg ccaagcttca
     1261 agacgagcaa ctacgcggcg atgatcgagc aggtccgccc cgacgtcgcc gcgctcgagc
     1321 aggtcgtgct catcggcact cggtcgtggg acgcgctcgt ggcgggcgcc ggcgaggtca
     1381 gcgcggcggc gctcgcggcc gtccaggccg gtctcgaccg caacgacgcg atcaacatcc
     1441 agtacacgtc tggcaccacc gggttcccca agggcgcgac gctgagccat cgcaacatcc
     1501 tcaacaacgg ctacctcgtc ggcgagatct gccgctacac cgaggtcgac cggatctgca
     1561 tccctgtgcc cttctaccac tgcttcggga tggtcatggg caacctcgcg gcgaccagcc
     1621 acggggcgtg catggtcatc cccgcccccg ggttcgaccc ggcggcgacg ctgcaggcca
     1681 cccaggacga gcgctgcacc tcg
//
LOCUS       DATAIX010000440         3103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_54041, whole genome shotgun sequence.
ACCESSION   DATAIX010000440 DATAIX010000000
VERSION     DATAIX010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54041"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VHM65_06615"
     CDS             complement(<1..627)
                     /locus_tag="VHM65_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2705413.1"
                     /translation="MSRADPPPPEAFRHDGAVAIVTGASRGIGYGVAQRLVSEGARVV
                     LTGRRPDALAEAVAALGGPERALGIAGNAADPEHQDEVIATTKATFGGIDYLVNNTGI
                     NPVYGPMLDTELEVARKILDVNVVSAFAWTQKAMHAGLGSARYPGAVVNVASIAGLGA
                     TALTRTRGASWKPSSVVRWMSAALETAYQPSTPVAPSPAIEATLTTAPG"
     gene            complement(624..1769)
                     /locus_tag="VHM65_06620"
     CDS             complement(624..1769)
                     /locus_tag="VHM65_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2705414.1"
                     /translation="MPRNIYDEEHEDFRASVREFVERTLKPRAEEFITDKTLPRDVWL
                     EAGKQGLLGLDIPEEYGGAGAADYRFNAVLTEELASFAAAASTCFGIHADVCPPYLVD
                     LGTEEQKQRWLPGMASGELICAIGMTEPSGGSDLAALKTTAIRDGDHYVVNGSKTFIT
                     NGYQGDLAIIAARTDPSKGNKGISLVMLESGMAGYTKGQKLDKVGQDESDTCELFFDN
                     VRVPVENLLGQEGRGFVSMMQRLSQERIGSAIANTAHAAQILTETIQYAKERKAFGQP
                     IGTFQHNKFKMAEMVTKIEVTQAYVDDCIIAHSKGTLSAVDAAKAKWWSSLVQGEVLD
                     DCVQLYGGYGFMNEYRVARAWRDARVTKIWAGSNEIMKELIGRDLGL"
     gene            complement(1906..2481)
                     /locus_tag="VHM65_06625"
     CDS             complement(1906..2481)
                     /locus_tag="VHM65_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705415.1"
                     /translation="MEPVLVVLVVAAGAVLAAVMAVVRWHVGRDMGPADSAETGHPGH
                     ARPARGGRLGGDSREASHPDDSDALLVPDDLAELDAAFAEISAESGVSVSVLLAARLQ
                     PLIARKVPIRAIRAASGPRSVRVQFADGTVVRARGTRAGDMGVLAQEVLQHSVRLTAL
                     SHETGQTHLVFSGPQTRRPIGAIALGLDQPE"
     gene            complement(2599..>3103)
                     /locus_tag="VHM65_06630"
     CDS             complement(2599..>3103)
                     /locus_tag="VHM65_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2705416.1"
                     /translation="ETLQVALQMWRGDDRPYTGRHYALDRPLNVPQALSKPHPPILIG
                     GGGEKKTLRLVAQYADACNIFDMGPAGVRAKYDVVQAHCADVGRDYGEIEKTVLSRVA
                     LGDGSPDPSGQPTTSVAEAVERLGRLAEVGTDHVIMGMANVAVPGTFHLVAELVRQAG
                     SITPAGR"
BASE COUNT          431 a         1081 c         1086 g          505 t
ORIGIN      
        1 cccgggggcc gtggtcaacg tcgcctcgat cgccgggctc ggcgcgaccg gagtgctcgg
       61 ctggtatgcc gtctccaagg cggcgctcat ccatctgacg accgagctcg gcttccagct
      121 cgcgccgcgg gtccgggtca acgcggtcgc gccgagcccg gcgatcgagg cgacgttgac
      181 tacggccccc ggatagcggg cggagccaag cccggcgtgc atcgccttct gtgtccacgc
      241 gaacgcgctg acgacgttga cgtcgaggat cttgcgggcg acctcgagct cggtgtcgag
      301 catcgggccg tagaccgggt tgatcccggt gttgttgacg aggtagtcga tcccgccgaa
      361 ggtggccttc gtcgtggcga tgacctcgtc ctggtgctcg gggtcggccg cgttgccggc
      421 gatgccgagg gcgcgctcgg gcccgccgag ggccgccacc gcctcggcca gcgcgtcggg
      481 cctgcgcccg gtgaggacga cgcgagcccc ttcgctgaca aggcgctgcg ccacgccata
      541 cccgatcccc cggctcgcgc cggtgacgat cgcgacggca ccgtcgtggc ggaaagcctc
      601 cggcggcggc gggtcagccc tgctcacaga ccgaggtccc ggccgatgag ctccttcatg
      661 atctcgttgg acccggccca gatcttggtg acgcgagcat cgcgccacgc ccgggcgacg
      721 cggtactcgt tcatgaagcc gtacccgccg tacagctgga cgcagtcgtc gaggacctcg
      781 ccctgcacca acgagctcca ccacttggcc ttcgccgcgt cgacggcgct gagcgtcccc
      841 ttcgagtggg cgatgatgca gtcgtcgaca tacgcctggg tgacctcgat cttggtgacc
      901 atctcggcca tcttgaactt gttgtgctgg aacgtcccga tgggctggcc gaaggccttg
      961 cgctccttcg cgtactggat cgtctcggtg aggatctgcg cggcgtgggc ggtgttggcg
     1021 atcgccgagc cgatgcgctc ctgggacagg cgctgcatca tcgagacgaa gcccctgccc
     1081 tcctggccga gcaggttctc caccggcacc cggacgttgt cgaagaacag ctcgcaggtg
     1141 tcggactcgt cctggccgac cttgtcgagc ttctggccct tggtgtagcc ggccataccc
     1201 gactcgagca tgacgaggct gatgcccttg ttgcccttgc tcgggtcggt ccgggcggcg
     1261 atgatcgcca ggtcgccctg gtagccgttg gtgatgaagg tcttggaccc gttgacgacg
     1321 tagtggtccc cgtcgcggat cgccgtcgtc ttcagcgcgg cgaggtcgga gcccccggac
     1381 ggctcggtca tgccgatcgc gcagatcagc tcgccggaag ccatcccggg cagccagcgc
     1441 tgcttctgct cctcggtgcc gaggtcgacg aggtaggggg ggcagacgtc ggcgtggatg
     1501 ccgaagcagg tcgacgcggc ggccgcgaag ctggccagct cctcggtgag gaccgcgttg
     1561 aaccggtagt cggcggcacc cgcgccgcca tactcctcgg ggatgtccag cccgagcagg
     1621 ccctgcttgc cggcctccag ccagacgtcg cggggcaggg tcttgtcggt gatgaactcc
     1681 tcggcgcggg gcttgagggt ccgctcgacg aactcacgca cgctcgcgcg gaagtcctcg
     1741 tgctcctcgt cgtagatgtt gcggggcatg ggcgacctcc gttgctactg acgggtacca
     1801 cggctctcgc ccaagtctcc ggggctcacc ccggcgtgtc aaaagaccgc tgtgaaggac
     1861 tcctggacaa cccagtcacg gctggacggg cgcgggtccg actcgtcatt cgggctggtc
     1921 gagacccagc gcgatggcgc cgatggggcg acgcgtctgc gggccggaga agaccaggtg
     1981 ggtctggccg gtctcgtggg acagggcggt caaccgaacg ctgtgctgca gcacctcctg
     2041 ggcgaggacc cccatgtcac cggctcgcgt gcctcgggcc cgcaccaccg tcccgtcggc
     2101 gaactgcacg cgcacgcttc gcggccccga ggccgcgcgg atggctcgga tcgggacctt
     2161 gcgcgcgatg agcggctgaa gccgggcggc cagcagcacg ctgacgctga ccccactctc
     2221 ggcgctgatc tcggcaaacg ccgcgtcgag ctcggcgagg tcgtcgggga ccagaagcgc
     2281 gtcggagtcg tcggggtgac tcgcctcccg ggagtcgccg ccgagtcggc cgccccgcgc
     2341 cggtcgcgca tggcccgggt gccccgtctc tgcactgtcg gccggtccca tgtcgcggcc
     2401 cacgtgccag cggacaacgg ccatcaccgc ggcaaggact gcccccgccg cgacgacgag
     2461 gacaacgagc accggctcca cggtctcagt gtgctccccc gtttgcctcg gcgaaagagc
     2521 agccacccgc aaaccccggg ctcgaacacc tgtgcgcggt ggggctcggt atcaccactc
     2581 aaggtgtcgg ctaggacgtc agcgaccggc gggagtgatc gacccggcct ggcggaccag
     2641 ctcggccacg aggtggaagg tccctggcac ggcaacgttg gccatcccca tgatgacgtg
     2701 gtcggtcccg acctcggcga gccggccgag ccgctcgact gcctccgcca cactggtcgt
     2761 cggctggccc gaagggtcag gtgacccgtc gcccagggcg acccggctca gtaccgtctt
     2821 ctcgatctcg ccgtagtcgc gcccgacgtc ggcgcagtga gcctggacga cgtcgtactt
     2881 ggcccggacc ccggccgggc ccatgtcgaa gatgttgcag gcatcggcat actgcgcgac
     2941 gagccgcagg gtcttcttct ctcccccgcc gccgatgagg atcggcggat gcggtttcga
     3001 cagtgcctga gggacgttga gcggtcggtc gagcgcgtag tgccggccgg tgtatggccg
     3061 gtcatccccc cgccacatct gcagggcgac ctgaagggtc tcc
//
LOCUS       DATAIX010000441         1995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_189301, whole genome shotgun sequence.
ACCESSION   DATAIX010000441 DATAIX010000000
VERSION     DATAIX010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189301"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VHM65_06635"
     CDS             <1..987
                     /locus_tag="VHM65_06635"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX2705417.1"
                     /translation="LARRICEHLGVRLSPVDIKRFSNDCLQAQLLTNCRLRDVYIVQP
                     LVPPTQESLMELLLMIDAARGASAAQITAVMPHYAYARSDKKDASRISLGGRLVADLL
                     VTAGVNRVLTMALHAPQVHGFFSVPVDHLTAIGVLADHYRGRDLTDTIVVSPDLGNAK
                     TATLFARLLGLSVAAGSKLRISDDKVVIDSIVGDVAGKRAIVLDDEIATGGSIVELLD
                     RLSDVGCTQASVACTHGLFAGPAVERLRGHPMITEVVTTDTVPPPVGWPELQVRSVAE
                     LFAEAIARIHKGKSVSSLFEGVDPAHAPPQPKLPFEDQAAFDELTIDLASAL"
     gene            1020..1376
                     /locus_tag="VHM65_06640"
     CDS             1020..1376
                     /locus_tag="VHM65_06640"
                     /inference="COORDINATES: protein motif:HMM:NF022700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HEX2705418.1"
                     /translation="MPLAAALAADLVAVTAFAAIGRASHAETGAAAAILLTAWPFLAG
                     LALGWAGVRVVSGHGPLTVRDAIPVWLSTVAVGMLLRALTGRGTAVSFIVVASIATGA
                     LLLGWRALAASRGARR"
     gene            1373..1900
                     /locus_tag="VHM65_06645"
     CDS             1373..1900
                     /locus_tag="VHM65_06645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2705419.1"
                     /translation="MTLPSREGEVTVRAALAEDVGAITVIYGHAVRHTVATFDLTEPD
                     AGYWLGRLASRSPGDHVLVAEDGSGVIVGYAYSGAFRPRPAYARSRETSVYLHPEHTG
                     QGIGTRLYAELLGRLGADGVHLAVAVVALPNPASTALHRAMGFTEVGTLDEVGHKLGR
                     YVSTTWWQRRLDRTS"
BASE COUNT          250 a          772 c          664 g          309 t
ORIGIN      
        1 ctggcccggc gcatctgcga gcatctcggc gtccggctct cgccggtgga catcaagcgc
       61 ttcagcaacg actgcctgca ggcccagctg ctcaccaact gccgcctgcg tgacgtctac
      121 atcgtgcagc cgctcgtgcc ccccacgcag gagtcgctca tggagctgct gctcatgatc
      181 gacgctgccc ggggggcctc ggctgcacag atcacggccg tcatgcccca ctacgcgtac
      241 gcccgctcgg acaagaagga cgcctcccgg atctccctcg gggggcgcct ggtcgcagac
      301 ctgctggtga ccgcaggggt caaccgggtg ctgacgatgg ccctgcacgc cccgcaggtg
      361 cacggcttct tctccgtccc cgtcgaccac ctcaccgcga tcggggtgct cgccgaccac
      421 taccggggcc gtgacctcac cgacacgatc gtcgtctcgc ccgacctcgg caacgccaag
      481 accgcgacgc tgttcgcccg gctcctgggc ctgtccgtgg ccgcgggcag caagctgcgg
      541 atctccgacg acaaggtcgt catcgactcg atcgtcggtg acgtggccgg caagcgcgcg
      601 atcgtgctcg acgacgagat cgccaccggg ggctcgattg tcgagctgct cgaccgcctc
      661 agtgacgtcg gctgcaccca ggcgtccgtc gcgtgcacgc atggcctctt tgccgggcct
      721 gcggtcgagc ggctgcgcgg ccatccgatg atcaccgagg tcgtgacgac cgacaccgtg
      781 ccgccgccgg tcggctggcc cgagctgcag gtccgctcgg ttgccgagct cttcgccgag
      841 gcgatcgcgc ggattcacaa gggcaagtcg gtcagctcgc tgttcgaggg ggtcgacccc
      901 gcccacgctc caccgcagcc caagctcccc ttcgaggacc aggccgcgtt cgacgagctg
      961 accatcgacc tggcctccgc gctctgatgc gagcctcgcc tgcggccccg agtcccagcg
     1021 tgcctctcgc agcggccctc gccgccgacc tggtcgctgt cacggccttc gccgccatcg
     1081 ggcgagccag ccacgccgag accggcgcgg ccgcggccat cctgctcacc gcgtggccct
     1141 tcctggctgg gctggccctc ggctgggccg gggtccgcgt ggtgtccggc catggcccgc
     1201 tcaccgtgcg tgacgccata ccggtgtggc tctcgaccgt cgccgtcggc atgctcctgc
     1261 gcgccctgac cggccggggt accgccgtct cgttcatcgt cgtggcctcg atcgccaccg
     1321 gtgcgctcct gctcggctgg cgcgccctcg ccgcgagccg cggcgcccgt cggtgaccct
     1381 gccctcccgg gagggcgagg tcacggttcg cgccgcgctc gccgaggacg tcggcgccat
     1441 caccgtgatc tacggccacg cggtgcgtca taccgtcgcc accttcgacc tcaccgagcc
     1501 ggacgcgggc tactggctcg ggcggctggc cagccgcagc cctggggacc acgtcctcgt
     1561 ggccgaggac gggtcggggg tgatcgtcgg ctatgcctac tccggcgcct tccggccccg
     1621 gccggcctac gcgcgctctc gggagacctc ggtctacctg caccctgagc acaccggtca
     1681 ggggatcggg acccggttgt atgccgagct gctcgggcgc ttgggcgcgg acggggtcca
     1741 cctcgccgtc gcggtcgtcg ccctgcccaa cccggcgagc acggcgctgc accgcgccat
     1801 ggggttcacc gaggtcggca cgctcgacga ggtcggtcac aagctcggcc gctacgtcag
     1861 caccacgtgg tggcagcgcc gactcgaccg cacgagctag gtccggctga gcataggcga
     1921 gcggcacgga ccgaccctgg agtgccgcgg atgagcctcc ctgtcgcctc cgcgcgcagg
     1981 ttactgacac accag
//
LOCUS       DATAIX010000442         1972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_84200, whole genome shotgun sequence.
ACCESSION   DATAIX010000442 DATAIX010000000
VERSION     DATAIX010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84200"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1898)
                     /gene="ppk1"
                     /locus_tag="VHM65_06650"
     CDS             complement(<1..1898)
                     /gene="ppk1"
                     /locus_tag="VHM65_06650"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX2705420.1"
                     /translation="MSTAETMIAEQAAAADVTAGATSGRDLTAVEVHVRSRVSAIAPG
                     ARPRAANGRFVREAPSRLVPTTDALPEGRFLEREISWLQFNERVLQLAMDETTPLLER
                     ARFLAIFASNLDEFYMVRVAGLKRRLATGLAVRSASGLEPRELLDQITHLSQELQRLQ
                     AHVFLSDIRPALSDEGIAIIRWDELTTDELTPLHGVFRDQVFPVLTPLAVDPAHPFPY
                     ISGLSLNLAVVLVNPKTGKRHFARVKIPDILARFFKVDDEHDPAHADLHHTRFLPLED
                     LVAAHLDQLFPGMEVREHYSFRVTRNEDFEVEEDDVENLLTVLEKELTRRRFGPPVRL
                     EVEQDMGERVLDLLCRELGVSAAEVCALPTPLDLRGLHQIADTERSDLHFDPFVPRTH
                     PDLSPTERSKPADVFAAVRTQDVLLQHPYDSFSTSVQAFIEQAAADPRVLAIKQTLYR
                     TSGDSPIVDALIDAADAGKQVLAVVEIKARFDEVNNISWARKLEQAGVHVVYGIVGLK
                     THAKLCLVVRQEAEGLVRYCHIGTGNYNPKTARLYEDLGVLTCDPQVGEDMSRLFNQL
                     SGVAPKSRFRRLLVAPRTVRSGLVGLVDAEADRARADGTGRVRFKMNSIVDETLIDAL
                     YRASQAGVS"
BASE COUNT          301 a          622 c          740 g          309 t
ORIGIN      
        1 gacacccccg cctgggaagc gcggtagagg gcgtcgatga gggtctcgtc gacgatggag
       61 ttcatcttga accggacccg gccggtcccg tcggcccggg cacggtcggc ttcggcgtcg
      121 acgaggccga ccaggcccga ccggacggtc cgcggcgcca cgagaagccg gcggaaccgg
      181 ctcttgggcg cgaccccgct gagctggttg aacagcctcg acatgtcctc gccgacctgc
      241 gggtcacacg tgagcacccc gaggtcctcg tagaggcgag cggtcttggg gttgtagttg
      301 cccgtcccga tgtggcagta gcggaccagg ccctcggcct cctggcgcac gacgaggcac
      361 agcttggcgt gggtcttcag gcccacgatg ccgtagacga catggacgcc cgcctgctcg
      421 agcttgcgag cccagctgat gttgttgacc tcgtcgaagc gggccttgat ctcgacgacc
      481 gcgagcacct gcttgcctgc gtcggcggcg tcgatgagcg cgtcgacgat cggcgagtca
      541 cccgaggtgc ggtacagcgt ctgcttgatc gcgaggaccc gggggtcggc cgctgcctgc
      601 tcgatgaagg cctggaccga ggtcgagaac gaatcgtacg ggtgctgaag caggacgtcc
      661 tgggtccgga ccgcggcgaa gacgtcggcg ggcttggacc gctcggtcgg ggacaggtcc
      721 ggatgcgtcc gtggcacaaa agggtcgaag tgcaggtccg agcgctcggt gtcggcgatc
      781 tggtgcagcc cgcgcaggtc gagcggcgtg ggcagcgcgc acacttcggc ggcgctgaca
      841 ccgagctcgc ggcagagcag gtcgaggacg cgctcgccca tgtcctgctc gacctcgaga
      901 cggaccggcg ggccgaaccg gcgccgggtc agctccttct ccaggacggt gagcaggttc
      961 tcgacgtcgt cctcttcgac ctcgaagtcc tcgttgcggg tgacccggaa cgagtagtgc
     1021 tcgcggacct ccatgcccgg gaagagctgg tccaggtgtg cggcgacgag atcctccagc
     1081 ggcaggaacc gggtgtggtg caggtcggca tgcgcggggt cgtgctcgtc gtcgaccttg
     1141 aagaaccgcg ccaggatgtc ggggatcttg acccgcgcga agtgccgctt gccggtcttg
     1201 gggttgacca ggaccacggc aaggttgagc gacagcccgg agatgtaggg gaacgggtgc
     1261 gccgggtcga cggcgagggg cgtgagcacc gggaagacct ggtcgcggaa caccccgtgc
     1321 aggggggtga gctcgtcggt cgtgagctcg tcccaccgga tgatcgcgat gccctcgtcc
     1381 gacagcgccg ggcggatgtc gctgaggaag acgtgcgcct gaaggcgctg cagctcctgg
     1441 gacaggtggg tgatctggtc gaggagctca cgtggctcga ggcccgatgc cgaacggacg
     1501 gccaggcccg tcgcgagtcg ccgtttgagg cccgcgacgc gcaccatgta gaactcgtcg
     1561 aggttgctcg cgaagatggc gaggaagcgc gcccgctcca gcaggggcgt ggtctcgtcc
     1621 atggcgagct gcaggacgcg ctcgttgaac tggagccagg agatctcccg ctcgaggaag
     1681 cggccctcgg ggagggcatc agtggtgggc accagccggc tcggggcttc gcggacgaac
     1741 cgcccgttgg ccgcccgggg ccgcgccccc ggggcgatcg cgctcaccct cgagcggaca
     1801 tgtacctcca cagcggtgag gtcgcggccc gaggttgctc ctgcggtcac gtcggcggcc
     1861 gcggcctgct cggcgatcat ggtctcagcc gtgctcatgg gtcattctgt caccaatatc
     1921 gagttggcgc atcgggcgga aagtcctgct tcactcaggt cggagcaccg gg
//
LOCUS       DATAIX010000443         2418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_24057, whole genome shotgun sequence.
ACCESSION   DATAIX010000443 DATAIX010000000
VERSION     DATAIX010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24057"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1626)
                     /locus_tag="VHM65_06655"
     CDS             complement(<1..1626)
                     /locus_tag="VHM65_06655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEX2705421.1"
                     /translation="MTVTAKPPAGATAPAPPDLVTLTIDGRSVSVPKGTLVIRAAEQL
                     GISIPRFCDHPALDPVGACRQCLVEVATPAPDGTLRTMMGPNGQVKPQTSCTMTVTPG
                     MDVKTQATSPIADRAQHGVMELLLINHPLDCPVCDKGGECPLQNQAMSNGRAVSRFED
                     VKRTYPKPVNISSQVLLDRERCILCARCTRFSDQIAGDPFIALVERGALQQVGIYEDQ
                     PFQSYFSGNTVQICPVGALTGAAYRFRSRPFDLESTPSVCEHCASGCAIRTDHRRGVV
                     LRRMAVLDPAVNDEWNCDKGRWAFAYANLADRITEPLVRGEDGELHVASWPEALAAAA
                     GGLRAAPSSGVLVGGRVSAEDAYAYGKFARTVLRTNSVDFRARPHSAEEAEFLASTVV
                     ATGPDNGAVTYATLGAATTVLLVGFEPEEESPIVFLRLRRAVRKNRTSVYAVAPYPTR
                     GLGKLGGTLLPAAPGTEAEVLRALAGADAGAPVAGATAALREPGAVILVGERLASVRG
                     ALSAAAALAEATGAGLAWVPRRAGERGARDPGQP"
     gene            complement(1623..>2418)
                     /locus_tag="VHM65_06660"
     CDS             complement(1623..>2418)
                     /locus_tag="VHM65_06660"
                     /inference="COORDINATES: protein motif:HMM:NF022059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX2705422.1"
                     /translation="SLEGRRGQPRLKPPFPGGAGLYARPTSVNNVETIASVPAIILHG
                     SDWFKAMGVPADPSANVQGSAGFGIFSLSGHVAHPGQYEAPLGITLRELLDLSGGMLD
                     PNKALKFWTPGGSSTPLFTAEHLDVPLDFDSVGAAGSMLGTRALQIFDETTCVVRAVD
                     RWTDFYQHESCGKCTPCREGTWWLKAILGRLERGQGSEEDLQKLLDICDTILGRSFCP
                     LGDGATSCITSSIQYFRDEYLAHLDQGGCPFDRDASSLFAKEKSRS"
BASE COUNT          334 a          814 c          944 g          326 t
ORIGIN      
        1 gggctggcct gggtcccgcg ccccgcgctc accggcgcgg cgcgggaccc aggccagccc
       61 cgctccggtg gcctcggcaa gcgcggccgc cgcggacagg gcgccacgca cggacgcgag
      121 acgctcaccg acgaggatga ccgcgcctgg ctcgcgcagt gcggcggtcg cgccggccac
      181 cggcgcgccg gcatcggcgc cggccagcgc ccgcaggacc tcggcctcgg tgcccggcgc
      241 cgccggcagc agcgtgccgc cgagcttgcc caggccccgc gtcgggtaag gcgcgacggc
      301 atacacgctg gtccggttct tgcggacggc ccggcgcagg cgcaggaaga cgatcggcga
      361 ctcctcctcg ggctcgaacc cgacgagcag gaccgtcgtg gccgcgccga gcgtggcgta
      421 ggtgaccgcg ccgttgtcgg ggccggtggc gacgaccgtg ctcgcgagga actcggcctc
      481 ctccgccgag tgcggacggg cgcggaagtc gacgctgttg gtgcgcagga cggtccgggc
      541 gaacttgccg taggcgtagg catcctcggc gctgacccgg ccaccgacga gcacgcccga
      601 gctcggtgcc gcgcgcagcc cgccagccgc cgcggcgagt gcctcgggcc acgacgcgac
      661 gtgcagctcg ccgtcctcgc cgcggacgag cggctcggtg atgcggtcgg ccaggttcgc
      721 gtaggcgaag gcccagcggc ccttgtcgca gttccactcg tcgttgacgg cggggtcgag
      781 cacggccatc cggcgcagca cgacaccgcg gcggtggtcg gtgcggatcg cgcagccgga
      841 cgcgcagtgc tcgcagacgc tcggggtcga ctcgaggtcg aacggccgcg accggaagcg
      901 gtaggccgcg ccggtcagcg ccccgaccgg gcagatctgg acggtgttgc ccgagaagta
      961 ggactggaac ggctggtcct cgtagatccc gacctgctgc agggcgccgc gctcgacgag
     1021 ggcgatgaac gggtcaccgg cgatctggtc ggagaaccgg gtgcaccggg cgcacaggat
     1081 gcagcgctcg cggtcgagca ggacctggga ggagatgttg accggcttgg ggtaggtccg
     1141 cttgacgtcc tcgaaccggg agaccgcgcg gccgttggac atcgcctggt tctgcagggg
     1201 gcactcgccg cccttgtcgc agaccgggca gtcgagcggg tggttgatga gcagcagctc
     1261 catgactccg tgctgggcgc ggtcggcgat cggggaggtg gcctgggtct tgacgtccat
     1321 gccgggggtc accgtcatcg tgcacgacgt ctgcggcttg acctggccgt tggggcccat
     1381 catcgtgcgc agcgtgccgt cgggggcagg cgtcgcgacc tcgacgaggc actggcggca
     1441 ggcaccgacc gggtccaggg cggggtggtc gcagaagcgc gggatcgaga tgcccagctg
     1501 ctcggcggcc cggatcacga gcgtgccctt ggggacgctc accgagcgcc cgtcgatggt
     1561 cagcgtgacc aggtcgggcg gtgccggtgc cgtggctccg gcgggcggct tcgcggtgac
     1621 tgtcatgaac gcgacttctc cttcgcgaac agcgacgagg cgtcccggtc gaacgggcac
     1681 ccgccctggt cgaggtgggc gaggtactcg tcacggaagt actgaatgct gctcgtgatg
     1741 cacgaggtgg caccgtcacc gagcgggcag aacgagcgcc cgaggatcgt gtcgcagatg
     1801 tcgaggagct tctgcaggtc ctcctcgctg ccctggccac gctcgaggcg gccgaggatc
     1861 gccttgagcc accacgtgcc ttcgcggcag ggggtgcact tgccgcagga ctcgtgctgg
     1921 tagaagtcgg tccagcggtc gacggcgcgc acgacgcagg tggtctcgtc gaagatctgc
     1981 agcgcccgcg tcccgagcat cgagccggcc gcgcccacgg agtcgaagtc cagtggcacg
     2041 tcgaggtgct cggcggtgaa gagcggggtc gaggacccgc ccggcgtcca gaacttgagc
     2101 gccttgttgg ggtcgagcat gccgccggac aggtcgagca gctcgcgcag ggtgatgccg
     2161 agcggggctt cgtactggcc agggtgcgcc acgtggccgg agaggctgaa gatcccgaag
     2221 ccggccgagc cctgcacgtt ggcgctcggg tccgcaggga cgcccatggc cttgaaccag
     2281 tccgagccgt gcaggatgat cgccggcacc gacgcgatcg tctcgacgtt gttgaccgag
     2341 gtcgggcggg cgtagaggcc cgcgcccccg gggaacggcg gcttgagccg gggctggccg
     2401 cggcggccct cgagcgag
//
LOCUS       DATAIX010000444         1691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_219219, whole genome shotgun sequence.
ACCESSION   DATAIX010000444 DATAIX010000000
VERSION     DATAIX010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219219"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /gene="fdxA"
                     /locus_tag="VHM65_06665"
     CDS             complement(<1..250)
                     /gene="fdxA"
                     /locus_tag="VHM65_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX2705423.1"
                     /translation="MTYVIAQPCVDIKDRACIEECPVDCIYEGERTLYIHPDECVDCG
                     ACEPVCPVEAIFYEDDTPEQWKDYYKANVEFFDDLGSPG"
     gene            383..1417
                     /locus_tag="VHM65_06670"
     CDS             383..1417
                     /locus_tag="VHM65_06670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2705424.1"
                     /translation="MPAAVKLTVGQRVMLRYRRPEGGEPPLTDVVGMLVEVGPDAVVV
                     QTRRGRVSIERRGIVAAKAVPPAPVRRGRPHRAIAIADLERVMASCWPPLESEPLGQW
                     VLRASNGFTRRANSLLPIGDPGLPLTEAVDLMQAWYDARGLRRVSALAGPAGFAIEDD
                     PLGELLLGRGYEPFNRTLVMTSATGSLMDVPADAPLVDVSDGPDEAWFSAWARDGVAL
                     TGTARQVIRGRPPARFASALRPDAATVGAGAPRGASRVVGIGQLMTAPGWGGLYSMYV
                     DPDERRRGVATAILAALAGAAGTLGIQSLYLQAAVANTAAVRRYTALGFATHHEYVYL
                     GSGPTGAPRG"
BASE COUNT          231 a          570 c          610 g          280 t
ORIGIN      
        1 ccccagggct accgaggtcg tcgaagaact cgacgttggc cttgtagtag tccttccact
       61 gctcgggggt gtcgtcctcg tagaagatgg cctcgaccgg gcagaccggc tcacaggcac
      121 cgcagtcgac gcactcgtcg gggtggatgt agagcgtgcg ctcaccctcg tagatgcagt
      181 cgaccgggca ttcctcgatg caggccctgt ccttgatgtc gacacacggc tgggcgatga
      241 cgtaggtcat gagtgctggg tcctcctgcg cgcggtgaga gtcaccccta gtatgccgag
      301 tcccggggca tcgggcacaa gaggccccgc cggattgggg cttggggtct ctccccgcgc
      361 ggatgcgcgg gaggatggaa ccgtgccagc agccgtcaag ctcaccgtcg ggcaacgggt
      421 catgctccgc taccgccgac cggagggcgg tgagccaccg ctcaccgatg tggtcgggat
      481 gctcgtcgag gtgggcccgg acgcggtcgt cgtgcagacc cggcgcggcc gggtgtcgat
      541 cgagcgccgg ggcatcgtcg ccgccaaggc cgtgccaccg gcgcccgtcc gccggggacg
      601 cccgcaccgg gccattgcca tcgccgacct ggaacgcgtc atggcgtcct gctggccgcc
      661 cctggagagc gagccgttgg ggcagtgggt gctgcgggcg agcaacgggt tcacgcgtcg
      721 cgcgaactcc cttctgccca ttggggatcc gggtctcccg ctgaccgagg ccgtcgatct
      781 gatgcaggcc tggtatgacg cacgagggct gcgccgggtc agcgccctgg ccggtccggc
      841 cggctttgcc attgaggacg acccgctcgg ggagctcctg ctggggcggg gctacgagcc
      901 cttcaaccgc acactggtga tgacgagcgc gaccggctcg ctcatggacg tgccggccga
      961 tgccccgctc gtcgatgtca gcgacgggcc ggacgaggcg tggttctccg cctgggcccg
     1021 cgacggcgtc gccctgaccg gcaccgcccg ccaggtgatc cgcggacgcc cgccggcccg
     1081 gttcgcgtcg gccctgcgtc ccgatgccgc gacggtcggg gccggtgcgc cgcgcggggc
     1141 gtctcgggtg gtgggcatcg gccagctcat gacggcgccc ggttggggtg gcctctactc
     1201 gatgtatgtc gacccggacg agcggcgacg gggcgtggcc accgcgatcc ttgcggccct
     1261 cgccggcgca gccggcacgc tcggcattca gtcgctgtac ctccaggccg cggtggccaa
     1321 cacggcggcg gtccgccgct acaccgcgct cgggtttgcc actcatcacg agtatgtgta
     1381 cctggggagc gggccgaccg gggcgccgcg cggctgatgg cgcctggctc gggggtccgc
     1441 gcgacacgcc gaggacttgt gagccagttg cgtttgacga gcccgtgttc tgtggtagtg
     1501 tttctgagct aggttcgcaa aagtgaacaa catctgaaca tcacgttaac ccaaggggat
     1561 atacgatatg agggctcttc tcaaggccta tgtcgcggac tacaggcagg tccagtgcgt
     1621 ggcgtgcctc gccgtgctgg caccgcgtcg gggtcgctga cgcccaactc atcatccagg
     1681 agcctccccc g
//
LOCUS       DATAIX010000445         5234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_113929, whole genome shotgun sequence.
ACCESSION   DATAIX010000445 DATAIX010000000
VERSION     DATAIX010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113929"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="VHM65_06675"
     CDS             complement(<1..922)
                     /locus_tag="VHM65_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX2705425.1"
                     /translation="MFLLCVWVLGLLPLWLVLAAFVSRFVPGRWRALRLAWFAAVYLA
                     LEVLVLVALFALWVRAGFGMTIRSEVSIGRHYRLMAWFLRRVLGSARFTFGLRVERDA
                     SPLDVAGPRPVLVLSRHAGAGDSFLLVDGIVNGPHHRRPRIVLKDLLQLDPAIDVLLN
                     RVGAGFVDSTRHGAAGMAQEIARLAAQAGARDAVVIFPEGANFTVDRRRRAIEKLEEI
                     DRPDLAERARRMVHLLPPKPLGALTAIEAAPGATVVFVGHAGLEAMSSVRDIWRNLPM
                     RHTVLVRAWVVPAENRPPAPEREVWLYDQWQ"
     gene            complement(973..1818)
                     /locus_tag="VHM65_06680"
     CDS             complement(973..1818)
                     /locus_tag="VHM65_06680"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX2705426.1"
                     /translation="MAFVFGGGGKWGAVQVGMLSALVERGVRPDVVVGCSVGALNGAA
                     FAGDPTPSGVATIRRLWEGAAGRAIAEATVLDHVRSVVGRRPYLYEPRRLREAVGSLT
                     HTSTFEDLDVPFQCVAASIEAAEEYWFTSGPLREALMASCAIPGLFPPVEIAGQHFYD
                     GGLVNSIPLDRAVALGSREVYVLQVGRVEQPLRPPRRLYEAPLVAFDIARRHRFATFR
                     TGLPAGVVLHVLPSGHRLVVDDPRQLAWRRLDGATDLITAAYEASVAYLDGIDIGIAP
                     DEVDS"
     gene            complement(1861..2841)
                     /locus_tag="VHM65_06685"
     CDS             complement(1861..2841)
                     /locus_tag="VHM65_06685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEX2705427.1"
                     /translation="MQVTQTVWYATIGVLTVLLIIDVVVIARRPHVPSMAETGRALAF
                     YLGLAVLFGLVVWRVWGGRYAGEYYAGWLTEYSLSVDNLFVFLLIMAKFGVPEKLQQS
                     ALLIGIIIAIILRGIFIALGAAVINSYAWVFYIFGAFLIYTAVRLGREGQTDDDEFEE
                     NRFLRWVEHRFPATQQYDGARLFTVENGKRLATPMLIVIIALGTTDLLFALDSIPAIY
                     GLTKEPYLVLSANIFALMGLRQLYFLIGGLLKRLVYLSIGLAVLLAFIGVKLILHALH
                     ENSLPFVNGGQPILGAPDIPISVSLGAILAILTVTTAASLMKSRRDAASA"
     gene            complement(2900..3268)
                     /locus_tag="VHM65_06690"
     CDS             complement(2900..3268)
                     /locus_tag="VHM65_06690"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX2705428.1"
                     /translation="MTGPGDPALGDTVRDYCLAKAGAWPDEPWEGDTVAKVGAKIFAF
                     LGSAHTVGLKCGATRAEADEWLLQYPGDASVMPYIGRFGWNVLRLDGAISAQEVLEAV
                     DASYEAVVARLPKAQRPVTR"
     gene            complement(3265..3582)
                     /locus_tag="VHM65_06695"
     CDS             complement(3265..3582)
                     /locus_tag="VHM65_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HEX2705429.1"
                     /translation="MSTAPARGPMHRVVDALATGAASRSELATMSGLPRDVADAVLDH
                     LVRIGRVGVDSLGVDCPDQGCGACPGGRAGAQAGCGSSGPGRSRGPVAMSLRPLLTRT
                     EPA"
     gene            complement(3579..>5234)
                     /gene="feoB"
                     /locus_tag="VHM65_06700"
     CDS             complement(3579..>5234)
                     /gene="feoB"
                     /locus_tag="VHM65_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEX2705430.1"
                     /translation="LLVDVPPDGRPDVVVVVADSTHLARSLYLVAELRERDRRVVVAL
                     TMVDIAARRGIVVDVDRLTASLGVPVVPVDPRRRTGTADLGAAVTRALAGPAPQPRPH
                     PGTGDTFELADERFAWVDAAVEPSVTTAAHVRRTWSDRIDRAVLHRVAGPVIFGAVMW
                     VVFQVTTTVAAPLQDVLDSLFSGPVSDAAVGTLGALHLDHPVVLGLVTNGLIAGVGML
                     LTFVPLMAIMFLLLAFLEDSGYLARAAVVADRAMRSMGLPGRAFLPLIVGFGCNVPAI
                     AATRVLTGARQRTLTALLVPFTSCSARLTVYVMLATAFFRPYAGTVVFAMYALSVVLV
                     VVVGLLLRRTLWRTMGSEPLLLDLPPYQRPTVRVMAGVTWVRLRGFLRTAGGIIVATV
                     AVVWLLQAIPVRGGEPFGEVAVPDSAYAQLAEAAAPVFAPAGFGQWEATGALVTGFVA
                     KEAVIASWAQTYAAQDSAGIADVSALGSAIRADFETSSDGHPNAAVLAFMVFLLAYTP
                     CVATVAAQWREIGAPWTLFGVGLQLAVAWILAVLVFQIGRVLT"
BASE COUNT          908 a         1789 c         1918 g          619 t
ORIGIN      
        1 cctgccactg gtcatagagc cagacctcgc gctccggcgc cggggggcgg ttctcggccg
       61 gcacgaccca ggcgcggacg agcacggtat gacgcatcgg caggttgcgc cagatgtccc
      121 tgaccgacga catcgcctcg agccctgcgt gcccgacgaa gacgaccgtg gcgcccgggg
      181 cggcctcgat cgccgtgagg gcaccgagcg gcttcggtgg cagcagatgc accatccgcc
      241 tggcccgctc ggcaaggtcg gggcggtcga tctcctcaag cttctcgatg gcacggcgtc
      301 gccggtcgac ggtgaagttg gctccctcgg ggaagatcac gaccgcatca cgggcccctg
      361 cctgagctgc cagccgggcg atctcctggg ccatgccggc ggccccgtgc ctcgtggagt
      421 cgacgaagcc tgcgcccacc cggttcagca ggacgtcgat cgccgggtcg agctgcaaca
      481 ggtccttgag cacgatccgc gggcggcggt ggtgagggcc gttgacgatc ccgtcgacca
      541 ggaggaacga gtcgccggca ccagcgtgcc ggctcagcac caggaccgga cgcggcccgg
      601 cgacgtccag tggactggca tctcgctcga cgcgcaggcc gaaggtgaac cgggcgctgc
      661 cgagaacccg gcgcaggaac cacgccatga ggcggtagtg gcgaccgatc gatacctcgc
      721 tgcggatcgt catgccgaag ccggcccgca cccacagggc gaacagcgcg accaggacga
      781 gcacttcgag cgcgagatag accgcggcga accaggccag gcgcagcgcc ctccatcgcc
      841 ccgggacgaa ccgggagacg aacgccgcga ggacgagcca gagtggcagc aggccgagga
      901 cccagacgca caggaggaac atcgcgggca cggtgacgac ccggcggacc cacctcggga
      961 gaaggaatag ccttatgagt ccacctcgtc cggtgcgatg ccgatgtcga tgccgtcaag
     1021 gtaggcgacg ctggcctcgt aggccgccgt gatcaggtca gtcgccccgt cgagccggcg
     1081 ccaggcgagc tggcgcggat cgtcgacgac gagccggtgg ccgctgggca ggacgtgcag
     1141 caccacgccc gccggcagac cggtgcggaa cgtcgcgaac cggtgccggc gcgcgatgtc
     1201 gaaggcaacc aggggcgctt cgtagagccg gcgcgggggt cgcagaggct gctcgacccg
     1261 cccgacctgg agcacgtaga cctccctcga cccgagggca accgcccggt ccagcgggat
     1321 ggagttgacc aggccgccgt catagaagtg ctgcccggcg atctcgaccg gggggaacaa
     1381 cccgggtatg gcgcatgagg ccatgagtgc ctcacgtaac ggcccggacg tgaaccagta
     1441 ttcctccgcg gcctcgatgg acgcggccac gcactggaac ggcacgtcca gatcctcgaa
     1501 ggtgctcgtg tgggtcagcg agccgacggc ctcgcgcagc cgccggggct cgtagagata
     1561 gggccggcgc ccgacgacag agcggacgtg gtcgagcacg gtggcctcgg cgatggcgcg
     1621 ccccgccgcc ccttcccaca gcctcctgat ggtggccacc ccggacggtg tcgggtcacc
     1681 ggcgaacgcc gcaccgttga gagcgccgac cgaacacccg accacgacgt cagggcgcac
     1741 gccacgctcg accagcgccg agagcatccc gacctgaacg gcaccccact tgccgccgcc
     1801 gccgaagacg aaggccaccc ggggcgcctt cgcctgactg ctcgccgccg acatggtcgg
     1861 ctacgccgag gccgcgtcgc ggcgcgactt catgaggctg gccgcggtcg tcaccgtcag
     1921 gatggcgagg atggcaccga gcgataccga gatggggatg tccggggcgc cgaggatcgg
     1981 ttgcccgccg ttgacgaacg gcaggctgtt ctcgtggagc gcatgcagga tgagcttgac
     2041 gccgatgaac gccaggagga ccgcaaggcc gattgagagg tagacgaggc gcttgagcag
     2101 gcccccgatg aggaagtaga gctggcgcag ccccatgagc gcgaagatgt tcgccgagag
     2161 gacgaggtac ggctccttgg tcagcccgta gatcgccggg atggagtcga gcgcgaagag
     2221 caggtcggtc gtgccgaggg cgatgatgac gatgagcatc ggcgtcgcga ggcgcttgcc
     2281 gttctcgacg gtgaacagcc gggcgccgtc atactgctgg gtcgcgggga agcggtgctc
     2341 gacccagcgc aggaaccggt tctcctcgaa ctcgtcgtcg tcggtctgcc cttcacgacc
     2401 cagccgcaca gcggtgtaga tgaggaaggc gccgaagatg tagaagaccc aggcgtagga
     2461 gttgatgacc gccgcgccga gtgcgatgaa gatgccgcgc aggatgatcg caatgatgat
     2521 accgatgagc agagccgact gctggagctt ctccggcacc ccgaacttgg ccatgatgag
     2581 caggaacacg aagaggttgt cgaccgagag cgagtactcg gtgagccagc cggcgtagta
     2641 ctcccccgca taccggccgc cccacacccg ccagacgacc aggccgaaca gcaccgcgag
     2701 gccgaggtag aaggccaggg cccgcccggt ctcggccatc gacgggacgt gcggacgtcg
     2761 ggcgatgacg acgacgtcga tgatgagcag cacggtgagc acgccgatcg tggcgtacca
     2821 gactgtctgc gtgacctgca tgaacgattc ctccggacgc gggagaagca tgaccggagg
     2881 tctccgccct gggccgatcc tagcgagtca ccggccgctg ggccttgggc agccgggcaa
     2941 cgaccgcctc gtatgacgcg tcgaccgcct cgaggacctc ctgcgcggag atcgccccgt
     3001 cgaggcgcag gacgttccag ccgaaccggc cgatgtaggg catgacggac gcgtccccgg
     3061 ggtactgcag gagccactcg tccgcctcgg cccgggtggc accgcacttg agccccacgg
     3121 tgtgcgccga gccgaggaag gcgaagatct tcgcccccac cttggccacg gtgtcgccct
     3181 cccacggctc gtcgggccag gccccggcct tggccaggca gtagtcgcgc acggtgtcgc
     3241 cgagggccgg gtccccgggt ccggtcacgc gggctcggtc ctggtcagca gcgggcgcag
     3301 gctcatcgcg accggcccgc gcgagcgccc ggggccgctc gacccgcagc ctgcctgggc
     3361 accggcgcgc ccgcccggac aggcaccgca gccctggtcg gggcagtcca cgcccaggct
     3421 gtccaccccg acccggccga tccgcacgag gtggtcgagg acggcatcgg cgacgtcgcg
     3481 cggcagcccg gacatggtcg cgagctccga gcggctggct gcccccgtcg cgagggcatc
     3541 gaccactcgg tgcatcggtc cacgagccgg ggccgtgctc acgtgaggac ccggccgatc
     3601 tggaagacga ggaccgccag gatccaggcg acggcgagct gcagaccgac gccgaagagg
     3661 gtccacggtg cgccgatctc gcgccactgg gcggccacgg tcgccacaca gggggtgtag
     3721 gcgagcagga agaccatgaa ggccaggacg gcggcgttcg ggtgaccgtc ggaggaggtc
     3781 tcgaagtcgg cccggatggc cgagccgagg gccgacacgt cggcgatgcc cgccgagtcc
     3841 tgggcggcat acgtctgcgc ccacgaggcg atgaccgcct ccttggccac gaagccggtc
     3901 acgagagctc cggtggcttc ccactggccg aagccggccg gggcgaacac cggggcagcg
     3961 gcctcggcca gctgcgcgta ggcgctgtcg gggaccgcca cctccccgaa gggctcgccg
     4021 ccgcgcaccg ggatcgcctg gagcagccac accacggcga ccgtggccac gatgatcccg
     4081 ccggctgtcc tcaggaagcc cctgaggcgg acccaggtca cgccggccat gacccgcacg
     4141 gtcggtcgct ggtagggcgg gaggtccagg agcaggggtt cgctccccat cgtgcgccac
     4201 agggtccggc gcaggagcag gccgacgacg acgacgagga cgacggacag ggcgtacatc
     4261 gcgaacacga ccgtcccggc atacgggcgg aagaaggccg tggccagcat gacgtagacg
     4321 gtgaggcggg ccgagcacga cgtgaacggc acgagcagcg ccgtgagggt tcgctgccgg
     4381 gcgccggtga gcacgcgggt cgcggcaatc gccgggacgt tgcacccgaa gcccacgatg
     4441 agcggcagga acgcccgccc aggcaggccc atcgagcgca tcgcccgatc ggccaccaca
     4501 gcggcgcggg cgagataccc ggagtcctcg aggaaggcga gcagaaggaa catgatcgcc
     4561 atgaggggca cgaaggtcag gagcatcccg actccggcga tgagcccgtt ggtgaccagg
     4621 cccaggacga ccggatgatc gagatggagg gcgccgagcg tgccgaccgc tgcgtcggac
     4681 accggcccgg agaagaggct gtcgagcacg tcctgaaggg gtgccgccac cgtcgtcgtc
     4741 acctggaaga ccacccacat caccgcaccg aagatcaccg gaccggccac ccggtgcagg
     4801 acggcccggt cgatccggtc gctccaggtg cgccggacgt gggccgcggt cgtgactgag
     4861 ggctcgacgg cggcatcgac ccaggcgaag cgctcgtcgg cgagctcgaa ggtgtcgccc
     4921 gtgcccggat gggggcgcgg ctggggcgcg gggccggcca gcgcgcgcgt gaccgccgca
     4981 ccgaggtctg ccgtgccggt gcggcgcctc ggatccactg gcacgacggg gacgccgagt
     5041 gaggcggtga gccggtcgac atcgacgacg atgccgcgac gcgcggcaat gtcgaccatc
     5101 gtcagcgcga cgaccactcg cctgtcacgt tcgcgcagct cggcgacgag gtagaggctc
     5161 cgggccaggt gggtcgagtc ggccacgacg acgaccacgt caggccggcc gtcggggggt
     5221 acgtcgacga ggag
//
LOCUS       DATAIX010000446         2100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_249017, whole genome shotgun sequence.
ACCESSION   DATAIX010000446 DATAIX010000000
VERSION     DATAIX010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249017"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..768
                     /locus_tag="VHM65_06705"
     CDS             163..768
                     /locus_tag="VHM65_06705"
                     /inference="COORDINATES: protein motif:HMM:NF040294.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5998 family protein"
                     /protein_id="HEX2705431.1"
                     /translation="MPGRSRVSAGSALPKDLLSSIERAGYYPALVADVVESALAGDET
                     VAHLVHQETTFDASSVRRHITVLALTPTRLVVAHADDFSDGEPHEAVATATSETIPIG
                     AVRGVMVTHVVPSPDQYVPGTLGREVTLTLGWGAVSRIDIMPGQCGDPSCDGEHGFEG
                     TMASDDISLRVSADADGDAALAAAITFARVLSSSIGSGTIR"
     gene            765..1895
                     /locus_tag="VHM65_06710"
     CDS             765..1895
                     /locus_tag="VHM65_06710"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase/phosphodiesterase
                     family protein"
                     /protein_id="HEX2705432.1"
                     /translation="MTGVDYPAESLAAVLPAVAGSLGSTRYAGPHPFALAPTRRAVVV
                     LVDGLGERLLRRRGGHAPFLRSHAASSRRLTCGFPSTTATSMGTFGTGLPPGSHGLVG
                     YEVLIPGEDRLLNELSWENGPDPFAWQPAPTVFEHVAAEGIDVTRVGPAFFDGSGLTN
                     AALRGGRFAAATALDARVAVAVAAVRATRRALVYLYWGDLDKVGHVYGVGSFEWGEEL
                     ESVDRALRTLAESVPSDTAVYITGDHGMVDAPFETRIDLAAEPALLAGVRHVGGEPRS
                     VQLYCQPGAQTDVEQVWRERLGEGAAVSTRSAMVHAGWFGDVRPEVEPRIGDVVACMR
                     GNLAVVHSGRMRPELLALLGLHGSTSQDELAIPLIGIPARSA"
BASE COUNT          253 a          795 c          735 g          317 t
ORIGIN      
        1 catcgacgac ctgccggaga tcgaggtggc cgagctcaac cccctcaacg cgtcgtccaa
       61 gggcatcgac atgctcggcg cccgggtcgt cgtccgtcca gcgccggtgc ggaccgagac
      121 cgaacggcgg gccctgtcct aacggcgtgg gaggatagcc caatgcctgg tcgcagccga
      181 gtctccgcag gttctgctct gcccaaggac ctgctgtcct cgatcgagcg ggccgggtac
      241 tacccggccc tggtcgccga cgtcgtggag tccgccctcg ccggtgacga aaccgtggcc
      301 cacctcgtgc accaggagac gacgttcgac gcgagctcgg tgcgccgaca catcaccgtc
      361 ctggccctga cccccaccag gctcgtcgtc gcccacgccg acgacttctc cgacggggag
      421 ccgcacgagg ccgtcgccac cgcgacgagc gagacgatcc cgatcggggc cgtccgcggc
      481 gtcatggtga cccacgtcgt gccctcgccc gaccagtacg tccccggcac cctcggccgc
      541 gaggtgaccc tcaccctcgg gtggggcgcc gtcagccgca tcgacatcat gcccggccag
      601 tgcggcgacc ccagctgcga cggcgagcac ggcttcgagg gcaccatggc ctccgacgac
      661 atcagcctgc gggtcagcgc cgacgccgac ggggacgcag ccctcgccgc agcgatcacc
      721 ttcgcccggg tcctgtcctc gtcgatcggg tccggcacga tccggtgacc ggagtcgact
      781 accccgccga gtcgctcgcc gctgtcctcc cggctgtcgc cgggtcgctc gggtccacgc
      841 ggtatgccgg gccgcatccg ttcgccctcg cccccacccg gcgcgccgtg gtcgtcctcg
      901 tcgacggcct cggcgagcgc ctgctccggc gccgcggcgg gcatgccccc ttcctgcgct
      961 cgcacgcggc ctcctcgcgg cggctcacct gtggtttccc ctcgacgacc gcgacctcga
     1021 tggggacgtt cgggacgggt ctgccccccg ggagccacgg gctcgtcggc tacgaggtgc
     1081 tgatccccgg cgaggaccgg ctcctcaacg agctctcctg ggagaacggc cccgacccgt
     1141 tcgcctggca gccggccccg accgtcttcg agcacgtcgc cgccgagggg atcgacgtga
     1201 cccgggtcgg accggcgttc ttcgacggct ccgggctgac caatgctgcg ctgcgcggcg
     1261 gccggttcgc cgcggccacc gcgctcgacg cgcgggtcgc ggtcgccgtg gccgccgtgc
     1321 gggcaacccg ccgcgcgctg gtctacctct actggggcga cctcgacaag gtcgggcacg
     1381 tctacggcgt tgggtcgttc gagtggggcg aggagctcga gtcggtcgac cgggcgttgc
     1441 gcacgctggc cgagtcggtc ccgtccgaca ccgcggtcta catcaccggc gaccacggca
     1501 tggtcgacgc cccgttcgag acccgcatcg acctcgcggc cgagcccgcc ctgctcgccg
     1561 gggtccgcca tgtcggcggt gagccgcgct cggtccagct ctactgtcag cctggggcgc
     1621 agaccgacgt cgagcaggta tggcgcgagc gcctcggtga aggggcggcg gtgtcgacgc
     1681 gctccgccat ggtgcacgcc ggctggttcg gggacgtgcg tcccgaggtc gagcctcgca
     1741 tcggtgacgt cgtcgcctgc atgcggggca acctcgcggt cgtccactcg ggtcggatgc
     1801 gcccggagct gctcgccctg ctcggcctgc acgggtcgac gtctcaggac gagcttgcga
     1861 tccccctcat cggtatacca gcccggtcgg cgtagagcgg ctgcctgtgg ctgagctcgc
     1921 cctcggcccg gaccatggac tgcggcacgt cgaccacccg ggccggctgg ttgagctggg
     1981 tccggccggt cagcggcggt cagatcggcc tcggtcgtgc ggggcgctat gactgaggtg
     2041 gatccggccg tctatggccg gtcagatcgg cctcggtcgt gcgggggcgg ttcactgagg
//
LOCUS       DATAIX010000447         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_114038, whole genome shotgun sequence.
ACCESSION   DATAIX010000447 DATAIX010000000
VERSION     DATAIX010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114038"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..1396
                     /locus_tag="VHM65_06715"
     CDS             320..1396
                     /locus_tag="VHM65_06715"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX2705433.1"
                     /translation="MRNPRDFFAPLAMGAPAPVREIPVRPSRVIHFFDPSNAKMAAKV
                     PDLVGSVDILLGNLEDGVKADNKLAAREGLVQIGTSTDFGEVTQLWTRLNSLDSPWFL
                     DDVTTLVPAIGDKLDVVMIPKVEGPEDIHYVDRLLAQLEAKAGLTRPILVHAILETAR
                     GMANVEQICAASPRMQGLSLGPADLAASRRMKTTRVGGGHPGYLVRQDPDPADETAVR
                     ATYQQDLWHYTIARMVDACAMNAILPFYGPFGDIKDVVACEDQFRNAFLLGCVGAWSL
                     HPVQIGIAKKVFSPSPADVAHARRVVEAMGDGTGAVMLDGKMEDDASLKQCRVLLELA
                     EQLSALDPGLASAYAGTPAGGEQQ"
     gene            1393..2274
                     /locus_tag="VHM65_06720"
     CDS             1393..2274
                     /locus_tag="VHM65_06720"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX2705434.1"
                     /translation="MTAPTHRPRRSVLYMPSSNERALEKAKTIPCDALILDLEDAVAP
                     DAKVAARAAACAAVESGDYGRRELTIRVNGVGTLWHWKDLMAVCAAGPDGIVVPKVRS
                     RKGVRDLVRAMERFNAPRHTKLWAMVETPDAILGAEQLAMASDRLAVLVMGTNDLVKE
                     LGAAHVPGRAPILPALAMALLAARAAGIAIVDGVYNNVRDAEGFEAECVQGRDMGFDG
                     KTLIHPGQVEVCNRVYAPSEAAIAEAHEIIAAFEAAVAEGRGVVTVNGRMIENLHVDI
                     ARKVLATDAAIRALEVG"
BASE COUNT          348 a          779 c          856 g          347 t
ORIGIN      
        1 gccgggatcg gcggcgaccg gttcgagcgg cagggctgtg gttggcggcg ctgcagaagc
       61 cggctctggg gcggcttcgg tgggcccggc tggctccggg gcggtcggct cgctctgcgt
      121 catgagccga gcctaggacc atggcggcac ctgccgccgc gccagcctcg gtatggcgtt
      181 cccggcgccg ctggggacgg gcgcgtcgtc gttgcgtgcg ggtggtcgtg aactgggtca
      241 tatcctcccg cgtgccgccg ctgctaggtt cggcagagac cgccgcgcag gtcggcaggt
      301 gccgtcacga ggagccatga tgcgcaatcc gagggacttc tttgccccgc tcgcgatggg
      361 tgccccggca ccggtccggg agatcccggt ccggcccagc cgggtcatcc acttcttcga
      421 cccgagcaat gccaagatgg cggccaaggt cccggacctc gtggggtcgg tcgacattct
      481 gctcggcaac ctcgaggacg gggtcaaggc ggacaacaag ctggccgccc gcgagggact
      541 cgtgcagatc ggcacgtcga cggacttcgg tgaggtcacc cagctgtgga cccggctgaa
      601 ctccttggac tcaccctggt tcctcgacga cgtgaccacg ctcgtgccgg cgatcggcga
      661 caagctcgac gtcgtgatga tccccaaggt cgagggaccc gaggacatcc actacgtcga
      721 ccggctgctc gcccagctcg aggccaaggc ggggctcacc cgcccgatcc tcgtccacgc
      781 gatcctcgag acagcccggg gcatggccaa cgtcgagcag atctgcgcgg cgtcgccccg
      841 gatgcagggc ctgtcactcg gccccgccga ccttgcggcg tcccggcgga tgaagacgac
      901 ccgggtcggc ggcggacacc ccggctacct cgtgcgccag gacccggacc cggctgacga
      961 gacagcggtg cgggcgacat accagcagga tctgtggcac tacacgatcg cccggatggt
     1021 cgacgcctgt gcgatgaacg ccatcctgcc cttctacggg ccgttcgggg acatcaagga
     1081 cgtcgtggcc tgcgaggacc agttccgcaa tgcgttcctg ctcgggtgtg tcggggcgtg
     1141 gagcctgcac ccggtgcaga tcgggattgc caagaaggtc ttcagcccgt cgccggccga
     1201 cgtagcgcac gcccggcggg tcgtcgaggc gatgggcgac gggacgggcg cggtcatgct
     1261 ggatggcaag atggaggacg acgcgagtct caagcagtgc cgtgtcctgc tggagctcgc
     1321 cgagcagctg tcggcgctgg accccggcct cgcgtcggcc tatgccggga ctccggctgg
     1381 aggggagcag caatgactgc accgacccac cgcccacggc ggtccgtgct ctacatgccg
     1441 tcctctaacg agcgggccct tgagaaggcc aagacgatcc cgtgcgacgc gctgatcctc
     1501 gacctcgagg acgcggtcgc gcccgacgcc aaggtcgccg cgcgggcggc ggcctgtgcg
     1561 gcggtcgagt ccggggacta cggtcggcgc gagctgacca tccgggtcaa cggagtcggc
     1621 acgctgtggc actggaaaga cctcatggcc gtgtgcgctg cggggccgga cggcatcgtc
     1681 gtgccgaagg tgcgatcgcg caagggggtt cgtgacctcg tgcgggcgat ggagcgcttc
     1741 aacgctccca ggcacaccaa gctctgggcg atggtcgaga cccccgatgc catcctcggg
     1801 gccgagcagc tggccatggc gtccgaccgg ctcgcggtgc tcgtcatggg gacgaacgac
     1861 ctggtcaagg agctcggtgc ggcgcatgtc ccagggcgcg cacccatcct gcccgccttg
     1921 gcgatggcgt tgctggcggc ccgggccgcg ggcatcgcga tcgtcgacgg ggtctacaac
     1981 aacgtccggg acgccgaggg cttcgaagcc gagtgcgtgc agggcaggga catgggcttt
     2041 gacggcaaga cgctgatcca ccccggtcag gtcgaggtgt gcaaccgggt ctacgccccg
     2101 agcgaggccg cgattgccga ggcgcacgag atcatcgcgg cgttcgaggc ggcggtggcc
     2161 gagggccgcg gggtcgtcac cgtcaacggg cgcatgatcg agaacctgca cgtcgacatc
     2221 gcccgcaagg tgctcgccac cgacgcggcg atccgcgccc tcgaggtggg ttaggtcagc
     2281 ccaggtggcg gccagccagt cgagctgggc cttcgcgtgt gagcgcgagt
//
LOCUS       DATAIX010000448         4517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_54451, whole genome shotgun sequence.
ACCESSION   DATAIX010000448 DATAIX010000000
VERSION     DATAIX010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54451"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1050..1826
                     /locus_tag="VHM65_06725"
     CDS             1050..1826
                     /locus_tag="VHM65_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705435.1"
                     /translation="MIGSSGSPQFDQLRRGFLADAALRQELEWAFRLAVETYNPSNRG
                     LRFITGGIGEWIITLAAYRTGLVTLPDGHDADGHDTLDLLAEVRELWSVKTSYKRSGK
                     FTITNGQGGPGAGLVVPTIFLSPDLPGIVYVSPRAHPSMVDRVEFGKDSSKLGKDVVR
                     DHAKRHLECVIEFDMPENPGAAVVDPALEAVRLLIDNLNFPRLRLLFQEARERKDVTV
                     VTQVKELKKMREDGTLDEAQYQSLMDQLTGVKYDRPQPRA"
     gene            complement(1932..4265)
                     /locus_tag="VHM65_06730"
     CDS             complement(1932..4265)
                     /locus_tag="VHM65_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX2705436.1"
                     /translation="MTNGGAQFGLTSAQVAERVARGEVNATVEVTSRPLGEIVKANVL
                     TRFNAVLGTLFVLVLLTGRYGDALFGWALILNSAFGIAQEWSAKRKLDSLAVLNVPTV
                     HVVRDGSEVEIATGAVVRDDVVALRAGDQVPADGPLLAGDNLEVDESNLTGESDAVAK
                     HPGDRLSSGTAVVAGHGSFRAEVVGEATEANTLSAQARKFRRAYSDVQHSINVLLRYL
                     TWAVVAAVPIVTWSQVRAVGDQGWREVVLRVAAALVGLIPEGLVLLTTLAFVVAAVRL
                     TRRHALIQELPAVEGLARVDIICLDKTGTLTVGDIAFDRVEAVPGIGMDEARAALGAI
                     AHVPDANATTGAIAAAVPDPGWPGDGGVPFSSARKWSAASFQGGGVWVLGAPDVILTA
                     CPDGPDRAWVQARTGELADTGDRVVLLARAPSLPVDDEPHDLAPTALVILAEQIRPDA
                     QETLDYFASQGVGVKVISGDNPRTVSAVARKVGLDPGTPVDARSLPDDPDALAEIVEA
                     TTVFGRVSPGQKRDLVRALQARGHVVAMTGDGVNDLLALKDADIGVAMGNAAPATKAV
                     AQLVLLDGRFAHLPSVLAEGRKVIGNVERVASLFVAKNIMSAVAILATALAGLGFPFL
                     PRHLTLLSTLTIGIPAAVLALGPNRRRYTPGLLGRVLRLSIPAGIAAGLVSFATYWLA
                     SSVGADTETQRGTAALLALLVVSFWLLAVLARPYLLWKVALIGTMVAAALAAFLVPAG
                     RRLFVLDVQGGTLVEALVIGGLGAVAVEVAYRASRRH"
     gene            complement(4302..>4517)
                     /locus_tag="VHM65_06735"
     CDS             complement(4302..>4517)
                     /locus_tag="VHM65_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2705437.1"
                     /translation="TDFRSDVAAIDVPALIAHGTADRILPIDATARPFHQLVPQAQYV
                     EVQGAPHGMLWTHSGEVNAALLGFLKP"
BASE COUNT          691 a         1572 c         1634 g          620 t
ORIGIN      
        1 gtaccggctc agctcggaag agacggcctg gatcggcgca tgggacgacc tcgtccagcg
       61 gatgtggagt gctcggggca cccggctgcc cgggttcccg ctgtgggccg acgcctggat
      121 cccggagcga gccctgcgcc ccgaggagat cgagcggctc ccgcggtgga aggctgactt
      181 cctggtcaag aacgcccgct tctacgacga ccaccgcgac ctcatcgacg actggcggcg
      241 ggcccatccg cagttccgtc gcttccccgg agtcccggcg caagctggag tggcaggccc
      301 aggacaccgg ctcctctggg agaccgtgat gtacttccgc ccggccggga tccgagccaa
      361 ggcgccgacc tacccgcctg ctacggggtc gctgtcggtg ctgcgtggta cctgctccga
      421 gagcacgtcg ccaaggacca ggcgtcgagg aagccgatgg ttccccagca cctgcgggac
      481 gccatactcg gtgccgatcc caatccctgc cccgggtcgt tggggccggc ggcctacgcc
      541 ttcgaagagc ggtggcgtgc cgaccgcgag gaacgggccg cgcgcagccg gaggctggtc
      601 ggctagcgtc gagccgtggt ggaacgctgg gagagcactc ccgaagggag gcacctcgcc
      661 ggtcgcgtca aagccggaac cgggcctgag gtcgcactgc gcaaggctct ccacgcggcc
      721 ggggctcgct tccgcattca gcggacgctg gctaagggct gcacgccgga cgtcgtgctc
      781 ccctcacgcc gcgtggccgt cttcgtcgac gggtgcttct ggcacggttg cccacagcac
      841 ggccgtcaga ccccctggac cggacccaac gcggagctgt gggaggccaa gatggcccgc
      901 aacgcggagc gagacgcgag gtcgaccaca ctcgctcagg acctgggctg ggaggtcgtc
      961 cgagtctggg aatgccaggt gcagacggac ccgggcgcgt gtgtcactgc ggtcctgcgc
     1021 gcacaatcgc cccacggtag cgtcccctca tgatcgggag cagcgggtcc ccgcagttcg
     1081 accagctccg tcggggattc ctggcggatg ccgcgctccg tcaggagctg gagtgggcct
     1141 tccgcctcgc cgtggagacc tataacccgt ccaatcgggg tctgcgtttc atcaccggcg
     1201 gcatcgggga gtggatcatc accctcgccg cctatcggac gggcctggtg acccttcccg
     1261 acggtcacga cgcggacggg cacgacacac tggatctcct cgccgaggtg cgcgagctgt
     1321 ggtcggtcaa gacgtcatac aagcgcagtg gcaagttcac gatcaccaac ggccagggcg
     1381 gacccggggc cgggctcgta gtcccgacga tctttctctc ccctgacctt ccgggcatcg
     1441 tctacgtcag tccgcgggca cacccctcga tggtggaccg ggtcgagttc ggcaaggact
     1501 cgtccaagct gggcaaagac gtggtgcgcg accacgccaa gcgccatctg gagtgcgtca
     1561 tcgagttcga catgcccgag aacccggggg cggctgtcgt tgacccggcc ctggaggcgg
     1621 tgcggctcct catcgacaac ctgaacttcc cccggctcag gctgttgttc caggaggcgc
     1681 gcgaacgcaa ggacgtcact gtggtcaccc aggtcaagga gctgaagaag atgcgggagg
     1741 acgggaccct cgacgaggct cagtaccaga gcctcatgga ccaactcacg ggtgtgaagt
     1801 acgaccgacc ccaaccgcgc gcctgatagt ggctcctcgg ggcgggaatt cgccgctcga
     1861 ggagccagta acctgcgcgg ggatggggcc cgggcaccga gggcgcggcc gaccccacat
     1921 gagtccggcg ctcagtggcg gcgggaggcg cggtaggcga cctcgacggc caccgcgccg
     1981 agcccgccga tcaccagcgc ttcgacgagg gtcccgccct gcacgtcgag cacgaagagc
     2041 cggcgcccgg ccggcacgag gaacgccgcg agggccgcgg cgaccatggt gccgatcagc
     2101 gcgaccttcc acagcaggta ggggcgcgcg aggaccgcga ggagccagaa cgagacgacg
     2161 agcagggcca gcaatgccgc cgtgccccgc tgagtctcgg tgtcggcgcc gacgctgctg
     2221 gccagccagt atgtcgcgaa gctcaccagc ccggccgcga tcccggccgg gatcgagagc
     2281 cgcaggacgc gcccgagcag gccgggggtg tatcggcggc ggttcggtcc gagggcgagg
     2341 accgccgcgg ggatgccgat cgtcagcgtc gacaggagcg tcagatgccg gggcaggaaa
     2401 gggaacccga ggccggcgag ggcggtcgcg aggatggcca cggcgctcat gatgttcttg
     2461 gcgacgaaca ggctggccac ccgctcgacg ttgccgatga ccttgcggcc ctcggcgagc
     2521 accgacggga ggtgggcgaa ccggccgtcg agcaggacga gctgggcgac tgccttggtg
     2581 gccggcgcgg cgttgcccat ggcgacaccg atgtcggcgt ccttgagcgc gagcaggtcg
     2641 ttgacgccgt cgccggtcat cgccacgacg tgcccgcggg cctgcagtgc gcgaacgagg
     2701 tcgcgcttct gtcccgggct cacccgcccg aagaccgtcg tcgcctcgac gatctcggcc
     2761 agtgcgtccg ggtcgtccgg cagggaccgg gcgtcgaccg gggtgccggg gtcgagcccg
     2821 accttgcggg ccaccgcgct gaccgtgcgg gggttgtcac cggagatgac cttgacgccg
     2881 accccctgcg aggcgaagta gtccagcgtc tcctgcgcgt cggggcggat ctgctcggcc
     2941 aggatgacga gcgccgtcgg tgcgaggtcg tgcggctcgt catcgacggg cagggacggc
     3001 gcccgggcca gcaggacgac ccggtcgccg gtgtcggcca gttcgcccgt gcgcgcctgc
     3061 acccaggcgc ggtccggccc gtcaggacag gcggtgagta tgacgtccgg cgcgccgagc
     3121 acccagaccc cgcccccctg gaaggaggcc gcgctccact tgcgcgccga gctgaagggc
     3181 accccaccgt cgcccggcca ccccgggtcg gggaccgcgg cggcgatggc ccccgtcgtc
     3241 gcgttcgcgt cgggcacgtg ggcgatggca ccgagcgccg cccgggcctc gtccatcccg
     3301 atgcccggca ccgcctccac ccggtcgaac gcgatgtccc ccaccgtgag cgtcccggtc
     3361 ttgtccaggc agatgatgtc cacccgggcc agaccctcga ccgcaggcag ctcctggatg
     3421 agggcgtggc gacgggtgag ccgcactgcc gcgacgacga acgcgagcgt ggtgagcagg
     3481 accaagccct ccgggatgag accgacgagc gcggccgcaa cccgcaggac gacctcacgc
     3541 catccctggt cgccgacggc ccggacctgc gaccacgtga cgatcgggac cgccgcgacg
     3601 accgcccacg tgaggtagcg cagcagcacg ttgatcgagt gctggacgtc cgagtacgcc
     3661 cggcggaact tcctggcctg cgccgacagg gtgttggcct cggtcgcctc cccgacgacc
     3721 tcggcccgga acgacccatg ccccgcgacg acggccgttc ccgagctgag ccggtcgccg
     3781 gggtgtttgg ccaccgcgtc ggactcgccg gtgaggttgg actcgtcgac ctcgaggttg
     3841 tccccggcga ggagcggtcc gtccgctgga acctggtcac cggcgcgcag cgcgacgacg
     3901 tcgtcgcgga ccaccgcacc ggtcgcgatc tcgacctctg acccgtcgcg gaccacatgg
     3961 acggtcggga cgttgaggac cgcgagcgag tcgagcttgc gcttggccga ccactcctgg
     4021 gcgattccga aggccgagtt gaggatgagg gcccagccga agagagcgtc gccataccgt
     4081 ccggtgagca gcacgaggac gaacagcgtg ccgagcaccg cgttgaagcg ggtgaggaca
     4141 ttggccttga cgatctcgcc caggggtcgc gaggtgacct cgacggtggc gttgacctcg
     4201 ccccgggcga cccgctccgc gacctgcgcg ctggtcaggc cgaactgggc tccgccattc
     4261 gtcacgcgcg ccagcctagt gacccgcgcc ggtcccctcg cctagggctt gaggaagccg
     4321 aggagcgcgg cgttgacctc gccgctgtga gtccacagca ttccgtgtgg ggccccctgc
     4381 acctctacat actgtgcctg cggcacgagc tggtggaacg gccgggcggt ggcgtcgatc
     4441 ggcaggatgc ggtcggcggt gccgtgggcg atcagggcgg ggacgtcgat ggcggcgacg
     4501 tcgctgcgga agtccgt
//
LOCUS       DATAIX010000449         2369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_84558, whole genome shotgun sequence.
ACCESSION   DATAIX010000449 DATAIX010000000
VERSION     DATAIX010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84558"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="VHM65_06740"
     CDS             <1..1247
                     /locus_tag="VHM65_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2705438.1"
                     /translation="LVRDKGVLARAESLDTGKRLVESEYDVEDVISCLRYYGGIAGTD
                     AGRVVDTGRADAISRVVHEPVGVCALITPWNYPLLQASWKVAPALLAGNTVVLKPSEL
                     TPSTSIQLIRLLAEAGLPDGVANLILGAGPAVGAALTEHPDVDMVSFTGGLATGRRIT
                     VAAAPTVKRVALELGGKNPNIVFADADREAALDNALTAVFLHSGQVCSAGARLVVEES
                     IHDAFVDDLVARASVIRLGGPFDPQAETGALVSEAHRTKVEEYVARGLAEGAVLRCGG
                     RRPDDPALAAGWFYEPTILDHCTTGMSCVQDESFGPVMTVETFTSEAEAIGIGNDTSY
                     GLASAVWTQDAGKAQRVALALRHGTVWINDYHPYVPQAEWGGFKQSGNGRELGPTGLA
                     EYRETKHIWQNIRPAPSGWFGQ"
     gene            1259..2317
                     /locus_tag="VHM65_06745"
     CDS             1259..2317
                     /locus_tag="VHM65_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX2705439.1"
                     /translation="MAPAAEPLIQVRDLWKVFGPRAAEVPGSPALRDLTRRELLDQTG
                     CTAAVREASFDVAEGEVFVIMGLSGSGKSTLIRCMSRLIEPTSGQLMFGGEDFLKADA
                     KALRDYRRDKISMVFQHFGLLPHRRVVDNVSFGLEIRGMGKLQRQKRALEVIELVGLS
                     GYDKAYPHELSGGMQQRVGLARALANDPDVLLFDEPFSALDPLIRRDMQAEVRRLHKE
                     VGKTMVFVTHDLSEALKLGDRILIMRDGNMIQLGTGDELVGAPADDYVRDFVQEVAKS
                     SVLTLRWIARAPRPHEDLSGPVLDKDVVIKDAIRAVLSSNAPVRVADGGDIVGVVGNE
                     EILVVISDQESVRAAGAG"
BASE COUNT          356 a          861 c          806 g          346 t
ORIGIN      
        1 tgctcgtccg cgacaagggc gtcctcgcgc gcgccgagag cctcgacacc ggcaagcgcc
       61 tcgtcgagag cgagtacgac gtcgaggacg tcatctcctg cctgcgctac tacggcggca
      121 tcgcgggcac cgacgccggg cgggtcgtcg acaccggccg ggccgatgcc atcagccggg
      181 tcgtccacga gccggtcggc gtgtgcgcgc tcatcacccc ctggaactac ccgcttctgc
      241 aggcatcgtg gaaggtcgcg ccggccctgc tcgccggcaa caccgtcgtc ctcaagccca
      301 gcgagctgac cccctcgacc tcgatccagc tcatccgcct gctggccgag gccggcctgc
      361 ccgacggcgt cgccaacctg atcctcggcg ccgggcccgc cgtcggcgca gccctcaccg
      421 agcaccctga cgtcgacatg gtgtccttca ccggcggcct cgcgacgggc cggcgcatca
      481 cggtcgccgc cgcacccacg gtcaagcggg tcgccctcga gctcggcggc aagaacccca
      541 acatcgtctt cgccgacgcc gaccgggagg ccgccctcga caacgcgctc accgcggtct
      601 tcctgcactc cgggcaggtg tgctcggccg gtgcccggct cgtcgtcgag gagtccatcc
      661 acgacgcgtt cgtcgacgat cttgtggcgc gggcgagcgt catacgcctc ggtggtccct
      721 tcgatcccca ggccgagacc ggggcgctcg tctcggaggc acaccgcacc aaggtcgagg
      781 agtatgtcgc ccggggcctc gccgagggcg cggtcctgcg ctgtggcgga cgccgcccgg
      841 acgatcctgc gctggcggcc gggtggttct acgagccgac gatcctcgac cactgcacca
      901 ccggtatgtc gtgcgtccag gacgagtcgt tcggcccggt catgaccgtg gagaccttca
      961 cgagcgaggc cgaggccatc ggcatcggca acgacacgtc atacgggctc gcgagtgcgg
     1021 tgtggaccca ggacgcgggc aaggcccagc gggtcgcgct ggccctgcgc catgggaccg
     1081 tgtggatcaa cgactaccac ccctatgtgc cacaagccga gtggggcggg ttcaagcagt
     1141 ccgggaacgg ccgtgagctc ggcccgaccg gcttggcgga gtaccgggag accaagcaca
     1201 tctggcagaa catccgaccc gcgcccagcg ggtggttcgg ccagtgagag gaggcgacgt
     1261 ggccccggcc gctgaaccgc tcatccaagt ccgcgacctg tggaaggtct tcggccctcg
     1321 cgccgccgaa gtccccgggt cccccgctct gcgtgacctg acccggcgcg agctgctcga
     1381 ccagaccggc tgcacggccg cggtccggga ggcctccttc gacgtcgccg agggcgaggt
     1441 cttcgtcatc atgggcctgt ccggctcggg caagtcgact ctcatccgct gtatgtcgcg
     1501 gctcatcgag ccgacctcgg gccagctcat gttcggcggc gaggacttcc tcaaggccga
     1561 cgccaaggcc ctgcgcgact accggcgcga caagatctcg atggtcttcc agcacttcgg
     1621 cctcttgccg caccgccggg tggtcgacaa cgtgtccttc ggcctggaga tccgcggcat
     1681 gggcaagctc cagcgccaga aacgcgcgct ggaggtcatc gagctcgtcg gtctctcggg
     1741 ctacgacaag gcctacccgc acgagctctc gggcggcatg cagcagcgcg tcggactggc
     1801 ccgcgcgctg gccaacgacc cggacgtcct gctcttcgac gagccgttct cggcgctcga
     1861 cccgctcatc cgccgggaca tgcaggccga ggtgaggcgg ctgcacaagg aggtgggcaa
     1921 gacgatggtg ttcgtcaccc acgacctgtc cgaggcgctc aaactcgggg accgcattct
     1981 catcatgcgc gacggcaaca tgatccagct cggcaccggc gacgagctgg tcggcgcccc
     2041 ggccgacgac tacgtccgcg acttcgtcca ggaggtggcc aagtccagcg tgctgacgct
     2101 gcgctggatc gcccgcgcgc cgcgcccgca cgaggatctc agcgggcccg tgctcgacaa
     2161 ggacgtcgtc atcaaggacg cgatccgggc ggtgctgtcg tccaacgcgc cggtgcgggt
     2221 cgccgacgga ggcgacatcg tcggagtcgt cggcaacgag gagatcctcg tcgtcatctc
     2281 cgaccaggag tcggtcaggg cggcgggggc aggatgagcc gcctcgacgg tatgccgggc
     2341 gcgcggctcg ggtgcgcgaa gacctgcac
//
LOCUS       DATAIX010000450         1984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_84594, whole genome shotgun sequence.
ACCESSION   DATAIX010000450 DATAIX010000000
VERSION     DATAIX010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84594"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VHM65_06750"
     CDS             complement(<1..622)
                     /locus_tag="VHM65_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705440.1"
                     /translation="MPGWYVHMEAAHETGRKLRAGDVPAGFAISAGEARDIGEMCHTW
                     RNYLALGSLGPDLFYLLPDFTNTKGAVLRQVVKWCLDVWEVVDSTFVSTWEKWIDPIS
                     TNSSQLASQLTGGLSSQLAQVLDELTSAIMSAFTGLLAQMGDWFGILTSGVPQGYGDD
                     GFYWSDMFHYRRTYQFPFVLFQQAKEALDQATTDAERHDAQARIAFA"
     gene            complement(1114..>1984)
                     /locus_tag="VHM65_06755"
     CDS             complement(1114..>1984)
                     /locus_tag="VHM65_06755"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEX2705441.1"
                     /translation="FVEAVSRESFPELGDVAGDIDAGRPVAVATVIEHPAETWLGRRM
                     VVRPESDPAAAAAPAPSGAMGLGSARADSAVTDDARGLLAAGRTETLTYGPDGERRGE
                     GMRVFVASYAPKPRMLVFGAIDFAAALARVGSFLGYHVTVCDARPVFATTSRFPSADE
                     VVVTWPHRYLGAEAQAGRVDGRTVIAVLTHDPKFDVPLLGVALRLPEVAYVGAMGSRR
                     THVDRLGRLRAAGITEAQIARLSSPIGLDLGARTPEETAVSIAAEIIAIRWGGRGHRL
                     TEVDGPIHLHAPR"
BASE COUNT          297 a          702 c          697 g          288 t
ORIGIN      
        1 cggcgaaggc gatccgggcc tgcgcatcgt ggcgctcggc gtcggtcgtg gcttggtcga
       61 gggcttcctt ggcctgctgg aagagcacga acgggaactg gtaggtccgc cggtagtgga
      121 acatgtccga ccagtagaag ccgtcgtcgc catacccctg ggggaccccg gaggtgagta
      181 tgccgaacca gtcccccatc tgcgcgagca ggcccgtgaa ggcgctcatg atcgccgagg
      241 tcagctcgtc gaggacctgg gcgagctggc tggacagccc gccggtgagc tgggaggcca
      301 gctgggagct gttcgtcgag atcgggtcga tccacttctc ccacgtactg acgaaggtgg
      361 agtcgacgac ctcccagacg tcgaggcacc acttcacgac ctggcggagc accgcgccct
      421 tggtgttggt gaagtccggc agcaggtaga acaggtccgg cccgagcgac ccgagggcca
      481 ggtagttgcg ccaggtgtgg cacatctccc cgatgtcgcg cgcctctccg gcgctgatcg
      541 cgaacccggc ggggacgtcc ccggcccgca gcttgcggcc ggtctcgtga gcggcctcca
      601 tgtggacgta ccaacctggc atgcgaggct ccttcgcggt gacccatgta tgacgcacga
      661 cgggttcgtc gcaccccttt ccaccgaaga gcgctcgtcg cgctcggccc cccggccgga
      721 cttcacgtag gccgaccctc acccggcgtg cgacgggatc cccaggtgct ctcgcaggta
      781 atggacgggc aatgtccgcg cctcgccatc atcggtgtac gtgccgccgg cgactgcatg
      841 gtcagcgtca ttgcgtcctc cacctcacgt gtccgtgtgc cgcatcgttg ccggttctcg
      901 acgcggtgac aagggtcgca cccgacggat ctcgctggcg gatcgcccga cggatgttgt
      961 tgtgctcggg tgcacgctcg agggccgtga gtcagcgggc gactcagcgc cgagtctccg
     1021 ccggcgacgg tggcgatccc actcaccggg ctgaaccaag cgacccggga actggcgccg
     1081 gaccgggctg ggccgtcacc aggaccggag gtctcagcgc ggcgcgtgga ggtggatggg
     1141 gccgtcgacc tcggtgaggc ggtgccccct gccgccccag cggatggcga tgatctctgc
     1201 ggcgatgctc acggccgtct cctcaggtgt ccgggccccg aggtcgagcc cgatcgggct
     1261 ggacagccgc gcgatctggg cctcggtgat gccggccgcc cggagccgcc ccaagcggtc
     1321 cacgtgcgtg cgccgcgaac ccatcgcgcc gacatacgcc acttcgggca ggcgcagggc
     1381 caccccgagg agggggacgt cgaacttcgg gtcgtgggtg agcacggcga tgaccgtccg
     1441 gccgtcgacc cggccggcct gcgcctcggc accgaggtag cggtgcggcc aggtcacgac
     1501 gacctcgtcg gccgaaggga agcggctggt ggtggcaaag acgggacgcg cgtcacacac
     1561 cgtgacgtgg tagccgagga aggacccgac ccgggccagg gcagcggcga agtcgatggc
     1621 cccgaagacg agcattcgcg gcttcggcgc gtatgacgcc acgaagaccc gcattccctc
     1681 gccgcggcgc tccccgtcgg gtccataggt cagcgtctcg gtcctcccgg ctgcgagcag
     1741 cccccgagcg tcgtcggtca ccgccgagtc cgcgcgagcg ctgcccagcc ccatcgcgcc
     1801 tgacggagca ggagccgcag ccgcagccgg gtcgctctcg ggacgcacga ccatccggcg
     1861 cccgagccac gtctccgccg gatgctcgat caccgtcgcc accgcgaccg gccgccccgc
     1921 gtcgatgtcg ccagcaacat ctcccagctc ggggaacgac tcgcgcgaga ccgcctcgac
     1981 gaac
//
LOCUS       DATAIX010000451         2831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_54635, whole genome shotgun sequence.
ACCESSION   DATAIX010000451 DATAIX010000000
VERSION     DATAIX010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54635"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..941)
                     /locus_tag="VHM65_06760"
     CDS             complement(60..941)
                     /locus_tag="VHM65_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX2705442.1"
                     /translation="MTAPSAPRRNTSGLSRDVEVLETLGLPEAIQSGGLGVVRIAAIT
                     GREKTVISRTLATLADAGLLSRDPGTLTYRLGPRLFALAARTAEATLVREARPHLRRI
                     ARHTNETTHLSVLRGGNVLTLVSELSPYEFRTAGWEGVTTAAWRTPSGRVLLSDWEDE
                     ELAAWYGQHGQDRPMVGPGRSDQSASRFAVLEAPAPESVVVRDLDDLRAEIARIRERG
                     YALSDEELEVGVVAASAPVTDFTGHIIAAVNVSAPKARIPGRLDALGEFVAKATQPLS
                     AHLGTWVSTTRGPGPRS"
     gene            1081..1740
                     /locus_tag="VHM65_06765"
     CDS             1081..1740
                     /locus_tag="VHM65_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2705443.1"
                     /translation="MDGLSGLPQFLQRRWSEILELTLQHLAVVALSLVLAMVVGIAIG
                     TLVWDRPVARSISITSAGIALTIPSLALLAILSPVLGLGWGPTVVALVFYSLLPIIRN
                     TVVGLREVPSEVLESARGMGMVPFGVLVRIQMPMAWPVILTGVRVAAQLTIGIAAIAA
                     YVAGPGLGQCIFRGLSSLGSRNALNYALTGTVGVVILALAVDAGFILITRLTTSRGLR
                     V"
     gene            1733..>2831
                     /locus_tag="VHM65_06770"
     CDS             1733..>2831
                     /locus_tag="VHM65_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2705444.1"
                     /translation="MSELTTSGTISALAGQHRGVSIELDSVTKSYPGQSISAVENFSL
                     RIEPGELVMLVGPSGCGKTTTMKMTNRIIGPTSGSIRIDGRDVLSINPNELRRHIGYV
                     IQQIGLFPHMTIAENIGAVPKLLGWPKARTAERVEELLRTVELDPSSFAQRYPRQLSG
                     GQQQRVGVARALAADPPVMLMDEPFGATDPITREKLQAEFLRLQSEIGKTIIFVTHDF
                     DEAIRLGDRIAVLSDRSRIEQFDTPAAILAAPANDYVRSFIGHGSALKRLALIPVTAG
                     DLGAESPGAPHVSDSATLPSAYEATGMPRRLLLDLVSPGELLGEVTPEAAEATGIPAG
                     LPVYATANDKAVEALGSGLRDEDVVLLSLGTY"
BASE COUNT          409 a         1032 c          932 g          458 t
ORIGIN      
        1 gcactcatcg tggcctgggg tctgcgtcgg gcccgcgtgc tcgccgatgc ccaccgcgtc
       61 taggagcggg gacccggacc cctcgtcgtg ctgacccacg ttcccaggtg ggcggacagg
      121 ggctgggtcg ccttggccac gaactcgccg agcgcgtcga gccgtccggg aatccgggcc
      181 ttgggggcac tgacgttcac ggccgcgatg atgtggccgg tgaagtcggt gaccggcgcc
      241 gacgccgcga cgaccccgac ctcgagctcc tcgtcggaga gcgcgtagcc cctttcccgg
      301 attcgtgcga tctcggccct caggtcgtcc aggtcgcgaa ccaccacgga ctccggggct
      361 ggcgcctcga gaacggcgaa ccgcgacgcc gactgatccg acctgcccgg cccgaccatc
      421 ggccggtcct ggccgtgctg cccgtaccac gcggcgagct cctcgtcctc ccagtcgctc
      481 aggaggacgc ggccggacgg cgtacgccag gccgccgtcg tcacgccctc ccacccggcg
      541 gtccggaact cgtaggggct cagctcactg accagagtga ggacgtttcc cccccggagc
      601 accgacaggt gggtcgtctc gttggtatgc cgggcaatcc ggcgcaggtg gggacgggcc
      661 tcccggacga gcgtggcctc tgccgtgcgc gccgcgaggg cgaacagccg cggcccgagg
      721 cggtaggtca gcgtcccggg gtcgcgggac agcagcccgg catcggcgag cgtcgccaag
      781 gtcctcgaga tgaccgtctt ctcgcggccg gtgatcgccg cgatgcgcac gacgccgagg
      841 ccgccggact ggatcgcctc cggcagcccc agggtctcga gtacctcgac gtctcgggac
      901 agcccagagg tgttgcggcg cggggctgaa ggcgcagtca tgttcgtcac gcgccgagag
      961 tagcaccagc agttgtcata tgcgcaagcg gtattgcgcg tatgacccat tgaaccctaa
     1021 ggtcacctca cctcacctca cctcacctca cctcaacgaa gagagttagg gaccgcgccc
     1081 atggacggcc tcagtggtct gccgcagttc cttcagcgtc ggtggagcga gatcctagag
     1141 ctgacgctgc agcatctcgc cgtcgttgcg ctgtcattgg tgctcgccat ggtggtcggc
     1201 atcgccatcg gcacgctggt ctgggaccgg cccgtggccc ggtcgatctc catcacgtcc
     1261 gccggcattg ccttgaccat cccgtcgctg gcgctgctcg cgatcctcag cccggtgctc
     1321 gggctgggct gggggccaac cgtcgtggcg ctggtcttct actcgctcct gccgatcatc
     1381 cgaaacaccg tcgtgggcct gcgcgaggtt ccgagcgagg tcctggagtc ggcgcgcggc
     1441 atggggatgg tgcccttcgg cgtcctcgtc cggatccaga tgccgatggc ctggcccgtc
     1501 atcctcactg gtgtccgggt cgccgcccag ctgacgatcg gcattgcggc catcgccgcc
     1561 tacgtggccg gtcccggcct cggccagtgc atcttcaggg gcctgtcatc cctcggctca
     1621 cggaacgccc tgaactacgc gctcacgggc acggtcggcg tcgtcatcct cgccctcgcg
     1681 gtcgacgcgg gcttcatcct catcacccgg ctcactacct caaggggtct tcgtgtctga
     1741 gctcaccacc tctgggacca tctcagccct ggccggtcag caccgcggcg tgagcatcga
     1801 gctcgacagc gtgacgaaga gctacccggg ccagtcgatc tcggccgttg agaacttctc
     1861 cctgcgcatc gagccgggcg agctggtcat gctggtcggc ccgagcggct gcggcaagac
     1921 gacgacgatg aagatgacca accggatcat cgggccgacg tcggggtcga tccggatcga
     1981 cggccgtgat gtcctgtcga tcaacccgaa cgagctgagg cgccacatcg gctacgtcat
     2041 ccagcagatc ggcctcttcc cgcacatgac catcgcggag aacatcggcg ccgtgcccaa
     2101 gctgctcggg tggcccaagg cgagaaccgc cgagcgggtc gaggagctgc tccgcaccgt
     2161 cgagctcgac ccttcgagct tcgcccagcg ctacccgcgc cagctctcgg gcggtcaaca
     2221 gcagcgcgtc ggtgtggctc gcgcccttgc cgccgacccg ccggtgatgc tcatggacga
     2281 gcccttcggc gccaccgacc ccattacccg cgagaagctg caggcggagt tcctgcggct
     2341 gcagtccgag atcggcaaga cgatcatctt cgtcactcac gacttcgacg aggccatccg
     2401 tcttggcgat cggatcgcgg tgctcagcga ccgcagccgg atcgagcagt tcgacacccc
     2461 ggcagccatc ctcgctgcac cggccaacga ctacgtccgg tcgttcatcg gccacggctc
     2521 ggcgctcaag cggctcgccc tcatcccggt caccgcgggg gacctcggcg ccgagtcgcc
     2581 gggtgccccc cacgtgagcg actcggcgac cctcccctcg gcctacgagg ccaccggtat
     2641 gccgcgccgc ctccttctcg acctcgtctc cccgggggag ctcctcgggg aggtcacgcc
     2701 cgaggccgcg gaggcgaccg gcataccggc gggtttgccc gtctacgcca cggcgaacga
     2761 caaggccgtc gaggcgctcg gcagtggcct gcgggacgag gacgtcgtgc tcctgtcgct
     2821 cggcacctac a
//
LOCUS       DATAIX010000452         1680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_173865, whole genome shotgun sequence.
ACCESSION   DATAIX010000452 DATAIX010000000
VERSION     DATAIX010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173865"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VHM65_06775"
     CDS             complement(<1..219)
                     /locus_tag="VHM65_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705445.1"
                     /translation="MTFEPMWSAIEPIGRHHRTGGYRRFAWTREDHDLREWFAGELGR
                     RELGAVGDADLARVDRPADADPSAVVDRH"
     gene            complement(275..>1680)
                     /locus_tag="VHM65_06780"
     CDS             complement(275..>1680)
                     /locus_tag="VHM65_06780"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX2705446.1"
                     /translation="VMRTHEWAPRVLIANSNLVGDWATWPEFRRLEHLGLTMYGQMTA
                     GSWIYIGTQGIVQGTYETFAVIAERRYGGSLAGTLTLTGGCGGMGGAQPLAVTLNGGA
                     CLIVDVDPARLHRRVEHRYLDEVATDLDDAVAKALAARRDRRAWSVGVVGNAATVFPE
                     LLRRGVEIDIVTDQTSAHDPLSYLPEEISLADWAGYAERKPEEFTDRARASMAKHVKA
                     MVEFQDAGAEVFDYGNSIRDEARLGGYDRAFEFPGFVPAYIRPLFCEGKGPFRWVALS
                     GDPKDIAATDRAVLDLFPDNDRLHRWIRGAQERIAFQGLPARICWLGYGERDVAGLRF
                     NELVASGEISAPIVIGRDHLDCGSVASPYRETEAMLDGSDAIADWPLLNALVNTASGA
                     SWVSIHHGGGVGIGRSIHAGQVCVADGTELAAAKLARVLTNDPGMGVIRHVDAGYQLA
                     EDVAAAKGVRVPMREGQ"
BASE COUNT          241 a          589 c          615 g          235 t
ORIGIN      
        1 gtgtcgatcc accacggcgg aggggtcggc atcggccggt cgatccacgc gggccaggtc
       61 tgcgtcgccg acggcaccga gctcgcggcg gccaagctcg cccgcgaacc actcccgcag
      121 gtcgtggtcc tcgcgggtcc acgcgaagcg ccggtagccg ccggtgcggt gatgacggcc
      181 gatcggctcg atcgcggacc acatcggctc gaacgtcata gccccaacct atgctcgatg
      241 cgcccgcacg tgaccgatgt cacgaccctc gctgtcactg accctctcgc atcggcaccc
      301 ggacgccctt ggccgcggcg acgtcctcgg cgagctggta gcccgcgtcg acgtggcgga
      361 tgacgcccat gcccgggtcg ttggtcagga cgcgggcgag cttggccgcc gcgagctcgg
      421 tgccgtcggc gacgcagacc tggcccgcgt ggatcgaccg gccgatgccg acccctccgc
      481 cgtggtggat cgacacccac gaggcacctg aggcggtgtt gacgagggcg ttgagcaacg
      541 gccagtcggc gatggcatcg gacccgtcga gcatcgcctc ggtctcccgg taggggctcg
      601 cgaccgagcc gcagtcgagg tggtcccggc cgatgacgat cggggcgctg atctcgcccg
      661 acgcgacgag ctcgttgaag cgcagacccg ccacgtcgcg ctcgccatag ccgagccagc
      721 agatgcgagc gggcaggccc tggaaggcga tccgctcctg ggcgccgcgg atccagcggt
      781 gcagccggtc gttgtcgggg aacaggtcga gcacggcccg gtcggtcgcc gcgatgtcct
      841 tggggtcgcc cgagagcgcg acccagcgga acgggccctt gccctcgcag aagagcgggc
      901 ggatataggc cggcacgaag ccggggaact cgaacgcccg gtcgtagccg ccgaggcggg
      961 cctcgtcgcg gatcgagttg ccgtagtcga agacctcggc accggcgtcc tggaactcca
     1021 ccatggcctt gacgtgcttg gccatcgagg cccgggcccg gtcggtgaac tcctccggct
     1081 tgcgctcggc gtagccggcc cagtccgcca gcgagatctc ctcgggcagg taggacagcg
     1141 ggtcgtgggc gctcgtctgg tcggtgacga tgtcgatctc gacaccccgg cgcagcagct
     1201 cggggaagac cgtcgcggca ttgccgacga cgccgacgga ccaggcccga cggtcgcgtc
     1261 tggccgcgag ggccttggcg acggcgtcgt cgaggtcggt ggccacctcg tcgaggtagc
     1321 ggtgctcgac ccggcggtgc agccgcgccg ggtcgacgtc gacgatgagg caggcccctc
     1381 cgttgagggt caccgcgagc ggctgggcgc cgcccatccc gccacagccc ccggtgaggg
     1441 tgagcgtgcc ggccagggag ccgccatacc gccgctcggc gatgacagcg aaggtctcgt
     1501 aggtgccctg gacgatgcct tgcgtgccga tgtagatcca cgacccggcc gtcatctgcc
     1561 cgtacatcgt caggccgagg tgctcgaggc ggcggaactc cggccaggtc gcccagtcgc
     1621 cgaccaggtt ggagttggcg atgaggaccc ggggggccca ctcgtgggta cgcatgaccc
//
LOCUS       DATAIX010000453         1532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_219387, whole genome shotgun sequence.
ACCESSION   DATAIX010000453 DATAIX010000000
VERSION     DATAIX010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219387"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VHM65_06785"
     CDS             complement(<1..828)
                     /locus_tag="VHM65_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX2705447.1"
                     /translation="MDVARFGTSEARRVVQIRYGAAALDALDTAGLWAVVATFEGEVV
                     AAQLADVSPVEAPVAEPAGWAPLEGSWSTTLDATAYRLAVQEVRDRVAAGAVYQSNIC
                     RVMTHPLAADADLGGLELLLGHGNPAPYASRVKIAEADVDLVCASPELFLSRRGTRLA
                     SAPVKGTAAPGEPMLDKDYAENVMITDLVRNDLSHVCLPGTVDVEVLCGAEDHPGLSH
                     LVTTVAGRLRPDATWAEIFAATFPPGSVSGAPKSSALRAIADLEPGPRGPYCGAVGWV
                     "
     gene            complement(949..>1532)
                     /locus_tag="VHM65_06790"
     CDS             complement(949..>1532)
                     /locus_tag="VHM65_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705448.1"
                     /translation="EKGATLELTHGGPAVLPLLHAVAGGRLHDGEQITKVVAPELAEL
                     RAGVDIGSSQHLLTAFSSEGRSVVVDSARLAALAGELGWLTDDAAHDERDQRRGRAAR
                     VHPFAVAGGTRRLIDRLDPERLATVAWTSPQELAKALPEGLAHLLDVGELTAGALAAR
                     LGGPDRACHLLRLTQSDELGSLVGGYSVILRAD"
BASE COUNT          199 a          557 c          581 g          195 t
ORIGIN      
        1 cacccagccc accgctccgc agtagggccc tcgcggcccc ggctcgaggt cggcgatggc
       61 ccgcagggcg ctggacttgg gggcccccga gaccgagccc ggcgggaagg tcgccgcgaa
      121 gatctcggcc caggtcgcgt ccggtcgcag ccgcccggcc acggtggtga cgaggtgtga
      181 gaggccgggg tggtcctcgg cgccgcacag gacctcgacg tcgaccgtcc ccgggaggca
      241 gacgtgcgac aggtcgttgc gaacgaggtc ggtgatcatg acgttctcgg cgtagtcctt
      301 gtcgagcatc ggctcgcccg gagccgctgt gcccttgacc ggggccgacg ccagacgggt
      361 cccgcgacgg ctcaggaaca gctccggcga ggcgcagacg aggtcgacgt ccgcttcggc
      421 gatcttgacc cggctcgcgt acggcgcggg gttgccatgg cccagcagaa gctccaggcc
      481 gccgaggtcg gcatcggccg cgagcgggtg cgtcatgacc cggcagatgt tggactggta
      541 gaccgccccg gcggcaacgc ggtcgcggac ctcctggacg gccagccggt atgccgtcgc
      601 gtcgagcgtc gtgctccacg agccctccag cggcgcccag ccggccggct ctgcgaccgg
      661 cgcctcgacc gggctgacgt cggcgagctg cgccgcgacg acctcacctt cgaaggtggc
      721 aaccacggcc cagagacctg ccgtgtcgag cgcgtcgaga gccgccgcgc cataccggat
      781 ctggacgact cgccgggcct cgctggtccc gaaccgtgcc acgtccatcc gggcatccta
      841 ggctgtgcgg cggtcgcggc ggtcgcggcg gtcgcggcgg tcgcggcggt cgcggcggcc
      901 gcacagcgac gcgggcccgg tgcgggctcg tcccacggcg ggacgagctc agtcggcgcg
      961 caggatgacg gagtacccgc cgacgaggga ccccagctcg tcggactgcg tcagccggag
     1021 cagatggcag gctcggtcgg gtccgccgag gcgggcggcc agggcaccgg cggtgagctc
     1081 cccgacgtcg agcaggtgcg ccaggccctc gggcaatgcc tttgccaact cttgggggct
     1141 cgtccacgcc accgtggcaa gccgttccgg atcgagccgg tcgatgagcc ggcgggtccc
     1201 tccggccacc gcgaaggggt gcacccgggc tgccctcccc cggcgctggt cacgctcgtc
     1261 gtgcgccgcg tcgtcggtga gccagccgag ctcgccagcg agggcggcga ggcgagcgct
     1321 gtcgacgacg acgctgcggc cctcggacga gaaggcggtc agcaggtgct ggctcgaccc
     1381 gatgtcgacg cccgcccgca gctccgcgag ctcgggggcg acgaccttgg tgatctgctc
     1441 gccatcgtgc agccgcccgc ccgcgacggc atgcagcagc ggcaggacgg ccggcccgcc
     1501 gtgggtgagc tcgagtgtcg cgcccttctc ga
//
LOCUS       DATAIX010000454         2799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_24483, whole genome shotgun sequence.
ACCESSION   DATAIX010000454 DATAIX010000000
VERSION     DATAIX010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24483"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1564
                     /locus_tag="VHM65_06795"
     CDS             170..1564
                     /locus_tag="VHM65_06795"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2705449.1"
                     /translation="MDPLVDPLPLRGAGPRPRVAVVGSGVAGLVAGYLLSGRADVTLY
                     EADARLGGHAHTQQVEVDGHPVAVDTGFIVHNDRTYPTLVRLFAELGIETQETQMSMS
                     VRCDETGLEYAGARGLGGLFAQPRALARPAYLLMLAEVARFHRRARTVLALPETHAGA
                     GETLEVFLDRGGFSAFFRDHFMTPVVAAVWSTAPGVALEFPARYLFAFLEHHGMLTVF
                     GSPTWRTVVGGSQRYVERVAAALHEVRCSSPVTGLSETPDGVEITVGRGTTARYDAAV
                     VATHPHQALAILASPSTVQRDVLGAMTYSVNLAQLHTDTSLLPRAERARASWNYLRRP
                     ERDDVVVSYDLTRLQRLDRAASGARLILTLGAPDLVDPATVLSTTIYEHPRYTPEFVA
                     AQRSLPLIDTGRVVFAGAYHGWGFHEDGAASGARAAARLGAGWPEPVSAGGHEARPSA
                     HSDAVVATGHGATA"
     gene            1561..2313
                     /locus_tag="VHM65_06800"
     CDS             1561..2313
                     /locus_tag="VHM65_06800"
                     /inference="COORDINATES: protein motif:HMM:NF018770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HEX2705450.1"
                     /translation="MSAPATPRLYATTIQHTRTGPLAHAFTSRSYSWLVDLDALPRLG
                     GLLGWFARFEARDHVGDPGATLRANIDSFLGDRGVDLRGGRVLMLASARVAGYVFNPI
                     SVFWCLDPDEALRCVVVEVHNTYGGRHAYLVQTDDRGRATVDKELYVSPFNDVSGHYE
                     LTVPLPGERVRLSVVLRRPGHPPFVARVAGVTRPWGRRTLVGLLVRHPLEPLMVSARI
                     RRQGIGLWLRRLPVQPRPASDSAGRAARGSTT"
     gene            2310..>2799
                     /locus_tag="VHM65_06805"
     CDS             2310..>2799
                     /locus_tag="VHM65_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705451.1"
                     /translation="MTIGEQTREHDMTISEQIRADGAGARSGERPSYPSEVDAARWPD
                     IARVPRGLRSRFASRIAQTLTRQAVSRLPMRMVLPGGEVCGGAARDSAPTIVVHDPGH
                     FFARLGDSGLVGFGEAYMAGDWSSPDLAAVLTVMAAQMGSLVPMPLQKLRAMYVVRHP
                     DTE"
BASE COUNT          335 a         1031 c         1006 g          427 t
ORIGIN      
        1 ggtcgcgttc gccgccgcgg ccgggtttat ctgtctcggc gccggccacg ggcgcccggc
       61 accaatccag cgcgcgcctg cctccgaacc tctgatgcaa ggctgacccc ggagcacccg
      121 tttcgcgggc accgcccgcg cgccgttggg taaggagagt gtcgtcgccg tggatccgct
      181 cgtcgacccg ttgccgttgc gcggcgccgg cccgcggccc cgggtcgcgg ttgtcggctc
      241 gggcgtggcg ggtctggtcg cgggctacct cctctcgggt cgcgcggacg tgaccctcta
      301 cgaggccgac gcgcgcctcg gcgggcacgc ccacacccag caggtcgagg tcgacgggca
      361 tccggtggcg gtcgacacgg ggttcatcgt gcacaacgac cggacctacc cgaccctggt
      421 gcgcctcttc gccgagctcg gcatcgagac gcaggagacg cagatgtcga tgtcggtccg
      481 atgcgacgag accggtctgg agtacgccgg agcgcgtggc ctcggtgggc tgttcgccca
      541 gccgcgggcg ctcgcgcgcc cggcatacct gctcatgctc gcggaggtgg cccggttcca
      601 ccggcgtgcc cgcacggtgc tcgcgctgcc cgagacgcac gccggcgccg gcgagacgct
      661 ggaggtattt ctcgaccgcg ggggcttctc ggccttcttc cgcgaccact tcatgacccc
      721 ggtcgtcgcc gccgtgtggt cgaccgcccc cggcgtggcg ctggagttcc ccgcccgcta
      781 cctcttcgcc ttcctggagc accacgggat gctcacggtc ttcgggtccc cgacgtggcg
      841 gaccgtcgtc gggggatcgc agcggtatgt cgaacgggtg gccgcggcgc tgcacgaggt
      901 gcgctgctcg agcccggtga ccggcctctc cgagaccccg gacggggtcg agatcaccgt
      961 gggccgtggc acgacggcgc ggtatgacgc ggccgtcgtc gcgacccacc cgcaccaggc
     1021 gctggcgatc ctggcctcgc cgtcgacggt ccagcgagat gtacttggtg ccatgacgta
     1081 ctccgtcaac ctcgcgcagc tgcacacgga cacgtcgctg ttgccccgcg ccgagcgggc
     1141 gcgggcctcc tggaactacc tgcgccgacc cgagcgcgac gatgtcgtcg tgagctacga
     1201 cctgacccgg ctgcagcgcc tcgaccgcgc ggcctccggg gcccggctca tcctgaccct
     1261 gggcgccccg gacctggtcg accccgcgac ggtgctctcg acgacgatct acgagcatcc
     1321 ccgctacacg ccggagttcg ttgcggcaca acgcagcctg ccgctcatcg atacgggccg
     1381 tgtcgtgttt gccggtgctt accacgggtg ggggttccac gaggacggcg ccgcctcggg
     1441 cgctcgggcg gccgctcgcc tcggggcagg gtggcccgag ccggtctcgg ccggggggca
     1501 cgaggcgcgt ccatcggccc acagcgacgc ggtcgtcgcg accggtcacg gtgcgaccgc
     1561 gtgagcgcac cagccacccc gcggctctac gcgacgacga tccagcacac ccggaccggc
     1621 ccgctggccc atgccttcac ctcacggagc tattcgtggc tcgtcgacct cgacgcgctc
     1681 cctcggctgg gggggctcct cggctggttc gcgcgcttcg aggcccgcga ccatgtcggg
     1741 gatccggggg ccaccttgcg ggcgaacatc gactccttcc tcggtgaccg cggcgtcgac
     1801 ctgcgcggtg ggcgggtcct catgctcgcc agcgcccgag tggccgggta tgtcttcaac
     1861 ccgatcagcg tgttctggtg cctcgacccg gacgaggcgc tccggtgcgt cgtcgtcgag
     1921 gtccacaaca cgtatggcgg gcgccacgcc tacctcgtcc agacggacga ccgggggcgt
     1981 gcgaccgtgg acaaggagct ctacgtctca ccgttcaacg acgtctccgg acactacgag
     2041 ctgaccgtcc cgctgcccgg cgagcgagtg cgcctgagcg tcgtcctgcg ccgcccgggt
     2101 catcccccgt tcgtggcccg ggttgccggg gtgacccgcc cgtggggccg gcgcacgctc
     2161 gtcgggctgc tcgtgcgcca tccgctcgag ccgctcatgg tgtcggccag gatcaggcga
     2221 caggggatcg gcctgtggct gcgccggctg ccggtccagc cccggccggc gtcggactcg
     2281 gcggggcgcg cggcgcgcgg gagcacgaca tgacgatcgg cgagcagacc cgggagcacg
     2341 acatgacgat cagcgagcag atccgggcag atggggccgg ggcgcgcagt ggcgagcggc
     2401 cgtcataccc gagcgaggtt gacgctgcgc gctggcctga cattgcccgt gtcccccgcg
     2461 gcctgcggag ccgctttgcg tcgcggatcg cgcagacgct gacgcgccag gccgtctcgc
     2521 ggctcccgat gcggatggtc ctgcccggcg gtgaggtgtg tggcggggct gcccgcgact
     2581 cggcgccgac catcgtcgtc cacgacccgg ggcacttctt cgcccggctc ggggacagcg
     2641 ggctcgtcgg gttcggggag gcatacatgg ccggggactg gtcctcgccc gacctcgcgg
     2701 cggtgctcac cgtcatggcc gcgcagatgg gctcgctcgt gccgatgccg ctccagaagc
     2761 tccgggcgat gtatgtcgta cgccatccgg acaccgaac
//
LOCUS       DATAIX010000455         2308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_84796, whole genome shotgun sequence.
ACCESSION   DATAIX010000455 DATAIX010000000
VERSION     DATAIX010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84796"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VHM65_06810"
     CDS             <1..337
                     /locus_tag="VHM65_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705452.1"
                     /translation="HAVKGARFFGYNRVGRVRWFTYGEVVAADPGRNLAFEITFGPVK
                     VSRWEYYFLPNQDGTCTLAEEWTDRRPTWFRVPATKAFGDRTRSNTRGVHRTLQAVKL
                     VAEGSPEAG"
     gene            402..1043
                     /locus_tag="VHM65_06815"
     CDS             402..1043
                     /locus_tag="VHM65_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX2705453.1"
                     /translation="MPSSSHLLAFAVAAFILIQIPGPSLLFTIGRALSVGRRDALLSV
                     LGNSAGVFVQVAAVATGVGAVVATSAQVYTVLKLAGAAYLIWLGVTAIRSRRDLTTAL
                     LDGRGRALPTWKVLRTGFTVGVTNPKTIVFFAALLPQFIDADRGMPALQMVVLGAIFC
                     AIAIVSDGAWALVAGRARDWFASSLHRLERIGGTGGVMMIGLGAALAVSGRPE"
     gene            complement(1234..2073)
                     /gene="rsmI"
                     /locus_tag="VHM65_06820"
     CDS             complement(1234..2073)
                     /gene="rsmI"
                     /locus_tag="VHM65_06820"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX2705454.1"
                     /translation="MDAAGVLVLAATPIGDPRDASPRLAGELASADVVAAEDTRRLRR
                     LVADLGVEVRGQVLSYHEHNESARTPDLVARLAAGDRVVLVTDAGMPSVSDPGYRLVT
                     AAVAAGIRVTGVPGPSAVLMALAVSGLPVDRFCFEGFVPRKPGERRRAFEALAGEHRT
                     MVFFEAPHRLPECLADLATAFGAERPGAVCRELTKTYEEVLRGTLGELAARVAGGVRG
                     EVTLVVAGAPVGVSTVAELLPGILARAGSGERLKQVCAEVAAATGRRPKELYDAAVAS
                     RAR"
BASE COUNT          301 a          854 c          821 g          332 t
ORIGIN      
        1 ccacgcggtc aagggggccc ggttcttcgg ctacaaccgg gtcggccggg tgcgatggtt
       61 cacgtatggc gaggtcgtcg cggccgaccc gggccgcaac ctcgccttcg agatcacctt
      121 cgggccggta aaggtctccc ggtgggagta ctacttcctg cccaaccagg acggcacgtg
      181 caccctcgcc gaggagtgga ccgaccgccg gccgacgtgg ttccgggtgc ccgcgaccaa
      241 ggctttcggg gaccggaccc ggtccaacac caggggagtc caccggaccc tccaggcggt
      301 caagctggtc gccgaaggct ccccggaagc cggctgagcc ctgctgggcg tcggcgagaa
      361 cgagttgccc gtgcgccggt gcgccagcca cactgggcct cgtgccctcg tcctcacatc
      421 tgctcgcgtt cgccgtcgcg gcgttcatcc tgatccagat cccggggccc agcctgctct
      481 tcacgatcgg gcgcgccctg tcggtcggtc gccgcgacgc cctgctgagc gtcctcggca
      541 actccgcggg cgtgttcgtc caggtcgccg cggtcgcgac cggcgtcggg gccgtcgtgg
      601 ccacctcggc gcaggtctac accgtgctca agctcgccgg cgcggcctac ctcatctggc
      661 tgggtgtcac cgcgatccgt agccggcgtg acctgaccac cgcgctgctc gacggtcgag
      721 gccgtgccct gccgacgtgg aaggtcctgc gcaccggctt caccgtgggc gtgaccaacc
      781 ccaagacgat cgtgttcttc gccgccctgc tgcctcagtt catcgacgcc gaccgaggca
      841 tgcccgccct ccagatggtc gtgctcggcg cgatcttctg cgccattgcc atcgtgtccg
      901 acggcgcctg ggcgttggtc gcgggccgtg cccgggactg gttcgcctcc tcgctgcacc
      961 ggctcgagcg gatcggcggc accggggggg tcatgatgat cggcctcggg gctgccctgg
     1021 ccgtgtccgg ccgccccgag taacctctcg accggtcgga gcgtgccgat ctggtcggcc
     1081 accgcatccg cgcgccgcaa agtggctcct cgcggcggac atatgctgcg cggggcgtga
     1141 ctaacctgcg cgcggatgag ccgagccggt tgcggtcggc cacagagagc gccccggcag
     1201 ttccagtcgc cagctccagc ttcccgccgc cagtcaccga gcgcgggacg cgactgccgc
     1261 gtcatacagc tccttgggcc gtcggccggt ggcggcggcg acctcggcgc agacctgctt
     1321 gagtcgttcc ccggagccag cccgggcgag tatgccgggc agcagctcgg cgaccgtgct
     1381 cacccccaca ggagccccgg cgaccacgag cgtgacctcc ccgcggacac cgcccgcgac
     1441 ccgcgcggcg agctcaccga gggtcccccg caggacctcc tcgtaggtct tggtcagctc
     1501 acggcagacc gcgcccgggc gctcggcacc gaaggcggtg gccaggtcgg caaggcactc
     1561 gggcagccgg tgcggggcct cgaagaacac catcgtgcgg tgctccccgg cgagggcctc
     1621 gaacgcccgc cgccgctccc ccggtttgcg cgggacgaac ccctcgaagc agaaccggtc
     1681 gacgggcagc cctgacacgg cgagcgccat gagcacggcg ctggggccgg gcaccccggt
     1741 gacccggatc ccggccgcca ccgccgccgt gacgagccgg taacccgggt cggagaccga
     1801 cggcatcccg gcgtcggtga cgaggacgac gcggtccccg gccgccaggc gcgcgacgag
     1861 gtccggcgtg cgcgccgact cgttgtgctc gtggtagctc aggacctggc cacgcacctc
     1921 gacgccgagg tcggcgacga gccggcgcag ccgccgggtg tcctcggcag cgacgacgtc
     1981 ggcggacgcg agctcgccgg ccaggcgagg cgaggcgtcg cgggggtcac cgatcggcgt
     2041 cgcagcaaga acgagcaccc cagcggcatc cacgccgtca tcctcccagg tcggggccgg
     2101 gccggtgccc cgtgaggtgg ctcccctgtg tgtccgtgag tcgagggtgt ccgtgggtcg
     2161 tggatgcccg tgggtcgggt gtgtcggtgg tgggtggcac gctggggagc atgacgacct
     2221 cactggacgt gtctggactg ctcgttgacc agatcgactg gcactggacc agccagctgc
     2281 gcccacggct cgacgggctc agcgacac
//
LOCUS       DATAIX010000456         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_189651, whole genome shotgun sequence.
ACCESSION   DATAIX010000456 DATAIX010000000
VERSION     DATAIX010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189651"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1185
                     /locus_tag="VHM65_06825"
     CDS             238..1185
                     /locus_tag="VHM65_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2705455.1"
                     /translation="MSDETTTSRAVPEATTSPLPGTDAAGPGPFGQAGVGQIGPTGRA
                     MPYFPEPALLASHGPARVIAMCNQKGGVGKTTTTINLGAALAEVGRRVLLVDFDPQGA
                     LSVGLGIQTHELDRTIYNLLTERGHDIREVIEHTRTPDLDVLPANIDLSAAEVQLVGE
                     VAREQTLARVLRPVLDDYDVILIDCQPSLGLLTVNALAASHGVLIPLECEFFAMRGVA
                     LLVETIDKITDRLNPRLQVDGILATMYDGRTLHSREVVRSVVDHFGDQVFHTVISRTV
                     KFPDATLAAEPITTYASTHSAAGAYRQLARELIARGGAA"
     gene            1190..2155
                     /locus_tag="VHM65_06830"
     CDS             1190..2155
                     /locus_tag="VHM65_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEX2705456.1"
                     /translation="MSEARAAASTEITPGGVITRRVPVRPFEVHLDVFEGPFDLLLGL
                     IAKHQLDITEIALAQVTDEFIGHIKARQAESEDWDLSQASEFLLVAATLLDLKATRLL
                     PSSGDEDEEDLALIEARDLLFARLLQYRAFKQVAAAFAERMAASGRTRARQAGLEPAF
                     AALLPELVMGITPEQLALIAARAMVPKPTPTVMLSHLHAPTVSVREQAAIIVARLRRT
                     GASSFRALVADADTTVLIVARFLALLELFRESVIAFEQAEALGELTVRWTGTEHGEIE
                     VSDEFDDGIDPEAEPAAEPEGDVKTQTLIETPRGDRPRRHTQGAH"
BASE COUNT          346 a          858 c          790 g          322 t
ORIGIN      
        1 gtatgccgaa gcgcaccccc gcgcccgctg actcgtgggg ccgcgctgaa accctcctcc
       61 ggggcgacct tcagcgcgac atccgacctc gggcgctcga atgcggcctt gtcgggagcc
      121 gggcgtgcct ggttccgaag gcgtcgcgtc cggccgcggc gggcccgcgc tgataaggtc
      181 gcatcgatca gcgagaggct gaccgcgacg aggttcgtga ctggggtgag cgaaacggtg
      241 agtgacgaga cgaccacgtc ccgggcggtg cctgaggcca cgacgtcccc tctgcccggc
      301 accgacgcgg ccgggccggg cccgttcggc caggccggag tcggccagat cgggccgacc
      361 gggcgagcga tgccgtactt ccccgagccg gcgctgctcg cctcccacgg gccggcccgg
      421 gtcatcgcca tgtgcaacca gaagggcggc gtgggcaaga ccacgacgac gatcaacctc
      481 ggagccgcac tggccgaggt cgggcgccgg gtccttctcg tcgacttcga cccgcagggg
      541 gcgctgtcgg tcggcctcgg gatccagaca cacgagctgg accggacgat ctacaacctg
      601 ctcaccgaac gcggccacga catccgcgag gtcatcgaac acacccggac cccggacctc
      661 gacgtcctcc cggccaacat cgacctgtcc gcagcggagg tccagctcgt cggtgaggtg
      721 gcccgcgagc agaccctcgc ccgggtcctg cgcccggtgc tcgatgacta cgacgtcatc
      781 ctcatcgact gccagccctc cctgggcctg ctcaccgtca acgccctggc cgcctcgcac
      841 ggggtcctca tcccgctgga gtgcgagttc ttcgccatgc gcggggtggc tctgctcgtc
      901 gagacgatcg acaagatcac cgaccggctc aacccccggc tgcaggtcga cggcatcctc
      961 gcgacgatgt acgacggacg cacgctgcac agccgcgagg tggtccgctc ggtcgtcgac
     1021 cacttcgggg atcaggtgtt ccacaccgtc atcagccgga ccgtgaagtt cccggacgcc
     1081 actctcgcgg ccgagccgat cacgacctac gcgtcgaccc actccgcggc cggggcctac
     1141 cgccagctcg cccgcgagct catcgcccgt ggcggggcgg cctgatccgg tgagcgaggc
     1201 gcgtgccgcc gccagcaccg agatcacccc gggaggggtc atcacccggc gagttccggt
     1261 gcgcccgttc gaggtgcacc tcgacgtctt cgagggcccg ttcgacctgc tcctcgggct
     1321 catcgccaag caccagctcg acatcaccga gatcgccctc gcccaggtca cggacgagtt
     1381 catcgggcac atcaaggccc ggcaggccga gagcgaggac tgggacctga gccaggcgag
     1441 cgagttcctg ctcgtcgccg ccacgctgct cgacctcaag gcgactcggc tgctgccttc
     1501 gtcgggggac gaggacgagg aggacctcgc gctcatcgag gcgcgggacc tgctcttcgc
     1561 ccggctgctg cagtaccgcg cgttcaagca ggtggcggcc gcgtttgccg agcgaatggc
     1621 cgcctcgggg cggacccggg cccgccaggc cgggctggag ccggcctttg ccgcgctgct
     1681 gcccgagctc gtcatgggca ttacccccga gcagctggcc ctgatcgccg cccgggccat
     1741 ggtgcccaaa cccacgccga cggtcatgct gagccacctc cacgccccga cggtcagcgt
     1801 ccgcgagcag gccgcgatca tcgtggctcg tctgcggcgc accggtgcca gctcgttccg
     1861 agccctcgtc gccgacgccg acacgacggt gctcatcgtg gcccggttcc tcgccctgct
     1921 cgagctcttc cgcgagtcgg tgatcgcgtt cgagcaggcc gaggcactcg gcgagctcac
     1981 cgtgcgctgg acggggacgg agcacggtga gatcgaggtc agcgacgagt tcgacgacgg
     2041 catcgacccc gaggccgagc ctgcggccga gcccgagggt gacgtgaaga cccagaccct
     2101 gatcgaaacg ccgcgcggcg atcgcccgcg acgacatacc caaggagccc actgatgagt
     2161 gaggacgtgt cctcgaccca gagccgcgag gagcccgagg acccggccgg cttcgacgcc
     2221 gccgacgtgc ccgggggggt gcgctcggcg atcgaggccg tcctcatggt cgtcgacgag
     2281 ccggtgtccg aggtcgcgct ggcctcggcg ctggag
//
LOCUS       DATAIX010000457         2370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_249256, whole genome shotgun sequence.
ACCESSION   DATAIX010000457 DATAIX010000000
VERSION     DATAIX010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249256"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VHM65_06835"
     CDS             complement(<1..755)
                     /locus_tag="VHM65_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705457.1"
                     /translation="MTHDPVPALGTRLTGRLVLPDEVVADGAVVLDGPTIVYAGPCAA
                     LPAHLGAVPEPVGWPAGRTLLPGLVDIHCHGGNGGEFGADVGASRVAAAHHHRRGTTT
                     VVASLVSHTRAALVGGVRTGAALVADGTLAGVHLEGPFLSYRYRGAQNPDALREVDYS
                     LLDDLVQAAGDRGDAIAQMTFAPERDVAGTLPRWLADHGITPAVGHTGASVGQVRAAL
                     DAAATSLGAAGSPLVTHVFNGMPSLHHREPGPVA"
     gene            complement(752..1657)
                     /locus_tag="VHM65_06840"
     CDS             complement(752..1657)
                     /locus_tag="VHM65_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX2705458.1"
                     /translation="MSTTGRTNLERELLTQADDWAAVVARVPALQPVLPHAGERVAVV
                     GCGTSYHMGLAYAERREAGGQGVTDAFPASEHHLDRGYDRVVLISRSGTTSEVMAVQA
                     ELITRGVATTTLVATAGTALTRGGGDVVLLSEVDESSVVQTRFATTALTLLRAGLGED
                     LAAAIADVRAVLDEPAETAVGHLAGVEQLTFLGRGWCVGLAMEAALKLRESAQLWTES
                     YPAMEYRHGPISIAAPGRAVWAFGEVPEGLAAEVEATGAVFEHRTIDPVAELVRVHRL
                     CVAAAGRRGLDPDVPRHLTRSIILS"
     gene            1800..>2370
                     /locus_tag="VHM65_06845"
     CDS             1800..>2370
                     /locus_tag="VHM65_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX2705459.1"
                     /translation="MPDHDRTGSMDRDQRWSALLGLLATFGRLTVAQVSAELGVSEAT
                     VRRDFTALAEQQLASRTHGGVVATAVAYELPARYKNATTDSAKERVAKTAAGLVPVGA
                     VVGLNGGTTTSATARHLGTRADLAEASARPALTVVTNALNIATELVLRPYIRCVTLGG
                     VARPESYEVTGPLARMVLEQLWLDVVIIGV"
BASE COUNT          346 a          872 c          851 g          301 t
ORIGIN      
        1 gcgacaggcc cgggctcacg gtggtgcagc gacggcatcc cgttgaagac gtgggtgacc
       61 agggggctcc cggcggcacc gagggaggtc gcggcggcgt ccagcgccgc tcgcacctgg
      121 ccgacggagg cgccggtgtg accgaccgcg ggggtgatgc cgtggtcggc gagccaccgt
      181 ggcagggttc ccgcgacgtc acgctccggg gcgaatgtca tctgcgcgat ggcgtcacca
      241 cggtcgcccg cggcctgcac gaggtcgtcg agcagggagt agtccacctc gcggagggca
      301 tcggggttct gggccccccg gtagcggtag gacaggaaag ggccctcgag atgcacgccg
      361 gccagagtgc cgtcggccac cagcgcggcg ccggtgcgga ccccacccac gagcgcggcc
      421 cgagtgtgtg acacgaggct ggccaccacg gtggtcgtgc cgcggcggtg gtggtgggcc
      481 gcggcgacgc ggctcgcgcc cacgtccgcc ccgaactcgc ctccgttacc gccatggcag
      541 tggatgtcca ccagtccggg caggagggtc cggcccgccg gccagcccac cggctcggga
      601 accgcaccaa ggtgcgcggg cagggcggcg cagggcccgg catacacgat ggtcgggccg
      661 tcgagcacga cggcgccgtc ggcaacgacc tcgtcgggca ggacgagcct tcccgtgagg
      721 cgggtgccca gggccggcac cgggtcgtgg gtcacgacaa gatgatggac ctggtcaggt
      781 gccggggcac gtccgggtcc agcccgcgcc ggccggccgc cgcgacgcag aggcggtgca
      841 ctcggaccag ctcggccacc gggtcgatgg tgcggtgctc gaacacggcc cccgttgcct
      901 cgacctcggc cgccaaaccc tcgggcacct caccgaaggc ccagacggcc cggcccggag
      961 cggcgatgct gatggggccg tggcggtact ccatggccgg gtaggactcg gtccacagct
     1021 gagccgactc gcgcagcttg agcgcggcct ccatggccag gccgacgcac cacccccggc
     1081 ccaggaaggt gagctgctcg accccggcga ggtgcccgac ggccgtctcg gccggctcgt
     1141 ccaggacggc ccgcacgtcg gcgatcgccg cggcgaggtc ctcgccgagt ccggcgcgga
     1201 gcagggtcag cgcagtcgtg gcaaaccggg tctgcaccac cgaggactcg tccacctcgc
     1261 tgagcagcac gacgtcgcca cccccgcggg tcagggccgt gcccgcggtg gcgaccagcg
     1321 tcgtcgtcgc aacgcctctg gtgatcagct ccgcctggac agccatcacc tcggacgtcg
     1381 tccccgaccg gctgatgagc acgacccggt cgtacccgcg gtcgaggtga tgctcgctgg
     1441 ccgggaacgc gtcggtcaca ccctgcccgc ctgcctcacg ccgctcggca tacgccaagc
     1501 ccatgtggta tgacgtcccg cacccaacga cggcaacgcg ttcgcccgcg tgcggcagga
     1561 ctggctgcag ggccgggacg cgcgccacca cggcagccca gtcgtcggcc tgggtgagga
     1621 gctcgcgctc cagattggtc ctgccggtgg tgctcatgac actcctgggg cctgcgtcag
     1681 ctggatgagg ggcaatgact gtataagatc gatcctcacc ataactgatc acgagtagtc
     1741 agacaacgcc gcacttgcca tacttccctc aagacccagg ctccgccaca ggaggcagca
     1801 tgcccgacca cgaccggacc ggcagcatgg accgagacca gcggtggagc gcgctcctgg
     1861 gcctgctggc caccttcggc cggctcaccg tcgcccaggt cagcgccgag ctcggcgtct
     1921 ccgaggcgac ggtccggcgt gacttcaccg ccctggccga gcagcagctc gccagccgga
     1981 cgcacggcgg ggtggtcgct acggccgtcg cctacgagct gccggctcgg tacaagaacg
     2041 cgacgacgga ctcggccaag gagcgagtcg ccaagacggc cgccgggctc gtcccggtcg
     2101 gggccgtcgt cggcctcaac gggggcacga cgaccagcgc gacggcgcgc catctcggga
     2161 cccgcgccga ccttgccgag gcctcggccc gcccggccct cacggtggtg accaacgccc
     2221 tcaacatcgc gaccgagctg gtcctgcgcc cctacattcg gtgtgtcacc ctcggtggcg
     2281 tcgcccgccc ggagtcctac gaggtgaccg ggcccctggc ccggatggtg ctcgagcagc
     2341 tctggctcga cgtggtcatc atcggcgtcg
//
LOCUS       DATAIX010000458         3453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_85058, whole genome shotgun sequence.
ACCESSION   DATAIX010000458 DATAIX010000000
VERSION     DATAIX010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85058"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1794)
                     /locus_tag="VHM65_06850"
     CDS             complement(67..1794)
                     /locus_tag="VHM65_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX2705460.1"
                     /translation="MEPNPAQVEALLAAIRESVIGEDQVMPGPYGPRRVTYADYTASG
                     RALGFIEDFIRDEVLPRYANTHTESSGTGLQTTRLREDARAIIRESVGAGVESAVIFA
                     GSGCTGAIDKLIGILGLRIPSTLEDRYALSAQIPADARPVVFIGPYEHHSNEVSWRET
                     IADVVVVPQDLDGHIDRAALLRGLTEYAGRPLRIGSFSAASNVTGIVTDTKAIATLLH
                     EHGALSFWDFAAAAPYIAIEMNAAPGEHPLAYKDAIFLSPHKFIGGPSTPGLLVIRRE
                     LCTNRVPDVPGGGTVAYVNPTEHRYLEDPEHREEGGTPAIIESIRAGLVFQLKAAVGV
                     EVIRAREAVFLARAVAGWQAEPTLQILGNLAAERLSIVSFVVHGPGGRYLHHNFVVAV
                     LNDLFGIQSRGGCSCAGPYGHYLLGIDLERSHEFEREISHGCEGIKPGWVRVNFNYFL
                     SDAVVDYIIEAVRIVARDGWRLLADYHFDPATGRWRHRKGPVEPPLRLRQVRYVDGRL
                     TFPRQEARAGEDALAGYLSEARAILESRYDGPVQSPDARVSADFDQLRWFELPQASLV
                     ETSPGDSSP"
     gene            complement(1962..>3453)
                     /locus_tag="VHM65_06855"
     CDS             complement(1962..>3453)
                     /locus_tag="VHM65_06855"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX2705461.1"
                     /translation="GKIVFDSHTAEALAKVGERVILVRRETNPDDLNGMIAAQGILTS
                     RGGKTSHAAVVARGMGKTCVCGAESLDVDVRGRRMTLPDGTVLTEGTLISIDGTSGEV
                     FLGEVPVSPSPVVQYFEGALHPSDGDELVMAVDRLMKVADRQARMTVRANADNGPDAE
                     RSRRFGATGIGLCRTEHMFLGDRRIYVEHLILADSPEEREAALGALLPLQRDDFIEIF
                     EAMDGLPVTIRLLDPPLHEFLPDITELSVRVAVAEANNDPAKADDLRLLGAVKRLHES
                     NPMLGLRGVRLGLVTQGLFGMQAQAILEAAAERIKAGGDPHVEIMVPLVGAVQELESI
                     KDEIVLVAKEVTERTGIDVKHLVGTMIELPRAALTAGEIAEVAEFFSFGTNDLTQTTW
                     GFSRDDVESAFFGHYIEQGIFAVSPFESIDIDGVGRLVRMGVERGRAARPGLKIGVCG
                     EHGGDPASVHFFNEVGLDYVSCSPFRVPVARLEAGRAAGQGGSDSR"
BASE COUNT          514 a         1154 c         1272 g          513 t
ORIGIN      
        1 acgttgaggt ggttccggcc ggtcagggtc ggccggatcg gcctcgctcg ggctgggggt
       61 gacgcgtcag gggctggagt cgcccgggct cgtctccacc aggcttgcct gcggcagctc
      121 gaaccagcgc agctggtcga agtccgcaga gacccgcgcg tcaggggact ggaccggccc
      181 gtcataccgc gactcgagga ttgccctggc ctcggagagg tagccggcca gcgcatcctc
      241 cccggccctg gcctcctgcc tcgggaaggt gagccgcccg tcgacatacc tgacctggcg
      301 cagccgcagg ggtggctcga ccggcccctt gcggtggcgc cagcgtccgg tcgccgggtc
      361 gaagtggtag tcggcaagca ggcgccagcc gtcgcgcgcg acgatgcgca ccgcctcgat
      421 gatgtagtcg acgaccgcgt cggacaggaa gtagttgaag ttgacccgga cccagccggg
      481 tttgatcccc tcgcagccgt gcgagatctc ccgctcgaac tcgtggctgc gttccaggtc
      541 gatcccgagc aggtagtggc cgtacgggcc cgcgcacgag cagccgcccc gcgactggat
      601 gccgaacagg tcgttgagga cggcgacgac gaagttgtgg tgcaggtagc gaccgccggg
      661 cccatggacg acgaaggaga cgatcgagag gcgctcggcc gcgaggttgc caaggatctg
      721 aagggtcggc tcggcctgcc acccggccac ggcccgggcg aggaagacgg cctccctggc
      781 ccgtatcacc tcgacgccga cggccgcctt gagctggaag accagcccgg cccggatcga
      841 ctcgatgatc gccggtgtgc cgccctcctc gcggtgttcg gggtcctcga ggtagcggtg
      901 ctcggtcggg ttgacgtagg ccaccgtccc cccgcccggg acgtccggca cgcggttggt
      961 gcacagctcg cgccggatca cgagcaggcc cggggttgac gggccgccga tgaacttgtg
     1021 cgggctgagg aagatggcgt ccttgtaggc cagggggtgc tcgccggggg cggcgttcat
     1081 ctcgatcgcg atgtaggggg cggcggccgc gaagtcccag aaggacaagg caccgtgctc
     1141 gtgcagcagc gtggcgattg ccttggtgtc agtgacgatc ccggtcacgt tgctcgccgc
     1201 ggagaacgac ccgatcctga gcggccggcc ggcatactcg gtcaggccac gaaggagcgc
     1261 ggcgcggtcg atgtgaccgt cgagatcctg cggtacgacg acgacgtcgg caatcgtctc
     1321 gcgccaggac acctcgttgg agtggtgctc gtagggcccg atgaacacga ccggtcgcgc
     1381 gtcggcgggg atctgcgcgc tgagcgcgta gcggtcctcc agcgtcgagg ggatgcgcag
     1441 tccgaggatc ccgatcagct tgtcgatcgc gccggtgcac ccggacccgg cgaagatgac
     1501 cgcgctctcc acgcccgccc cgaccgactc gcggatgatc gcccgcgcgt cctcacggag
     1561 ccgggtcgtc tgcaggccgg tcccggagga ctcggtgtgg gtgttggcat aacggggcag
     1621 gacctcgtcg cggatgaagt cctcgatgaa ccccagcgcc cggcccgagg cggtgtagtc
     1681 ggcataggtg acccggcgtg gcccgtaggg gccgggcatc acctggtcct cgccgatgac
     1741 cgactcgcgg atcgcggcga gcagggcctc gacctgcgcc gggttgggct ccacccggcc
     1801 caggataggc actgcttatg gggttgtcga cgcccataac ccgaggtgat gctcaggcgc
     1861 agtcggggtg cgacttcggg tgcgcggacc gggcctgtcc gcgtaaggcg cacaggcccg
     1921 atccatgacg cccgcagagg caggggcgcg agcccgtagg gtcagcggga gtcgctgccg
     1981 ccctggcccg cggcgcgacc ggcctcgagc cgtgcgacgg ggacgcggaa gggcgagcaa
     2041 gacacgtagt cgagcccgac ctcgttgaag aagtgcacgg aggcggggtc gcccccgtgc
     2101 tcgccgcaga cgccgatctt caggcccgga cgggccgccc ggccgcgctc gacacccatg
     2161 cggacgagac ggccgacgcc gtcgatgtcg atcgactcga acggggacac cgcgaagatg
     2221 ccctgctcga tgtagtgacc gaagaacgcc gactcgacgt cgtcacggga gaagccccac
     2281 gtcgtctggg tcaggtcgtt cgtgccgaac gagaagaact cggccacctc ggcgatctcg
     2341 cccgccgtga gggccgcccg ggggagctcg atcatcgtgc cgacgaggtg cttgacgtcg
     2401 atcccggtgc gctcggtgac ctccttggcc accaggacga tctcgtcctt gatgctctcc
     2461 agctcctgca cggcgccgac gagcggcacc atgatctcga cgtgcgggtc gccaccggcc
     2521 ttgatccgct cggccgcagc ctcgaggatc gcctgtgcct gcatgccgaa caggccctgg
     2581 gtcaccaggc cgaggcgcac cccacgcaga ccgagcatcg ggttggactc gtgcaggcgc
     2641 ttgaccgcac cgagcaggcg caggtcgtcg gccttggccg ggtcgttgtt ggcctcggcc
     2701 accgcgacgc gcacggacag ctcggtgatg tcggggagga actcgtgcag cggcgggtcg
     2761 agcaggcgga tcgtcaccgg cagcccgtcc atcgcctcga agatctcgat gaagtcgtcg
     2821 cgctgcagcg gcagcagcgc gccgagcgcg gcttctctct cctcggggga gtcggcgagg
     2881 atgagatgct cgacgtagat ccggcggtcg ccgaggaaca tgtgctcggt ccggcacagg
     2941 ccgatgccgg tcgcgccgaa gcggcgggag cgctccgcgt cgggcccgtt gtccgcgttg
     3001 gcgcggaccg tcatccgggc ctggcggtcg gcgaccttca tgagccggtc gacggccatg
     3061 acgagctcgt caccgtccga ggggtgcagc gctccctcga agtactgcac gaccggggac
     3121 ggggagaccg gaacctcgcc gaggaagacc tcgccggacg ttccgtcaat cgagatgagc
     3181 gtgccctcgg tgaggaccgt gccgtcgggg agggtcatcc tgcggccgcg gacgtcgacg
     3241 tcgagtgact cggctccgca cacgcaggtc ttgcccatgc ctcgggcgac gaccgccgcg
     3301 tgcgaggtct tgcctccgcg gctggtgaga atgccctggg cggcgatcat gccgttgagg
     3361 tcgtcggggt tggtctcgcg gcgcacgagg atgacccgct cgccgacctt ggccagggcc
     3421 tccgcggtgt gggagtcgaa gacgatcttg ccg
//
LOCUS       DATAIX010000459         3968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_130015, whole genome shotgun sequence.
ACCESSION   DATAIX010000459 DATAIX010000000
VERSION     DATAIX010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130015"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VHM65_06860"
     CDS             <1..235
                     /locus_tag="VHM65_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705462.1"
                     /translation="EVVELLRAEGGLGEEALERVRSAGLRVQLARRFHNDAVTDARRV
                     RRKVSVRVFRLAGHADMPRTFEFDDELPSAIPA"
     gene            357..1277
                     /gene="pdxS"
                     /locus_tag="VHM65_06865"
     CDS             357..1277
                     /gene="pdxS"
                     /locus_tag="VHM65_06865"
                     /inference="COORDINATES: protein motif:HMM:NF003215.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX2705463.1"
                     /translation="MAEQDSTRPTEAQHTVGTTRVKRGMAEMLKGGVIMDVVTAEQAT
                     IAEDAGAVAVMALERVPADIRAQGGVSRMSDPDMIDAIIAAVSIPVMAKARIGHFVEA
                     QVLQALGVDYVDESEVLTPADYANHIDKWAFTVPFVCGATNLGEALRRITEGAAMIRS
                     KGEAGTGDVSQATTHMREIRGQINRLRSMSSDELYVAAKELQAPYDLVADVARAGKLP
                     VVMFTAGGIATPADAAMMMQLGAEGVFVGSGIFKADNPAQRAEAIVKATTFYDDPDVI
                     AKVSRGLGEAMVGINVEDIPAPHRLAQRGW"
     gene            complement(1284..2552)
                     /gene="kynU"
                     /locus_tag="VHM65_06870"
     CDS             complement(1284..2552)
                     /gene="kynU"
                     /locus_tag="VHM65_06870"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEX2705464.1"
                     /translation="MTHSSLASTAAPGPLGARAAQLDAVHDATDRRSAFSLPADVVYL
                     DGNSLGALPAAVPEVVQRLVTQQWGDRLVRSWNEHDWWGAPERVGDLVGALIGASPGQ
                     VVVTDSTSVNLAKVYLAAARMRPGRDIVLIDPGSFPTDLYVLQSAAGYAGWSIELATP
                     DRAPERLRELGTRVALASYSSVDYRTGELWDLLAITEAAHDIGALASWDLCHSAGVLP
                     VGLDAHGVDFAVGCGYKYLNGGPGAPAFVYVAQRHLASFHNPLSGWHGHARPFEMGPL
                     YEPAPSITRARVGSPPLLSLLALEAALSAYDGLSIESVRARSLSLTSFFRECLDGLGA
                     DVDVVTPAADERRGSQVSLRHEQGLAVVQALIARGVIGDFRRPDIVRLGFAPLYLSHV
                     DVLTAARHVAEVLASREYERPEFQSRAAVT"
     gene            2611..3225
                     /gene="pdxT"
                     /locus_tag="VHM65_06875"
     CDS             2611..3225
                     /gene="pdxT"
                     /locus_tag="VHM65_06875"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX2705465.1"
                     /translation="MTVPRSPVVGVLAVQGDVREHLRALTALGARATSVRRPRELDQV
                     DALVIPGGESTTMDKLVRAFGLDEPLRRRIAEGMPVYGSCAGMIMLADRIADARPDQH
                     CLGGLDITVRRNAFGRQVDSFEEDLDFTGLAHPVHAVFIRAPWVEQFGAAVEVLARIE
                     AGPAAGRIVAVRQGALLATSFHPEVTGDTRVHAEFLTLVQASRS"
     gene            3236..>3968
                     /locus_tag="VHM65_06880"
     CDS             3236..>3968
                     /locus_tag="VHM65_06880"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX2705466.1"
                     /translation="MSGHSKWATTKHKKAVVDARRGALFAKLIKNIEVAARTGGGDPD
                     ANPTLWDAIYKAKKTSVPNDNIDRAVKRGSGVEAGGADWATIMYEGYAPGGVAVLVEC
                     LTDNRNRAAAAVRTALTRHGGSLADPGSVSYLFARKGVVMVPRGSLTEDDLLEVVLDA
                     GAEEINDIGEAFEIVSEPGDFVAVRTALQGAGIDYDSAEASFIPTVEVELDLDGARKM
                     FRVIEALEDSDDVQNVWANGDVSDKI"
BASE COUNT          640 a         1373 c         1405 g          550 t
ORIGIN      
        1 cgaggtcgtc gagctgctcc gggccgaggg cggcctcggc gaggaagccc tcgaacgagt
       61 ccgctcagct gggcttcggg tccagctggc ccgccggttc cacaacgacg cggtcaccga
      121 cgcgcgccgg gtgcgccgca aggtcagcgt gcgggtcttc cgtttggcgg ggcacgccga
      181 catgccccgg accttcgagt tcgacgacga gctgcccagc gccatacccg cctgacctgg
      241 ctcggccagc gcttggccgc cagcgccgtg ctcggtcgcg cgcggcgagg cgagataggc
      301 tggagtgctg aggcaggcgt gacatgaggc agcacacgag agcgagagca aggaccatgg
      361 cagagcagga cagcacccgg ccgaccgagg cgcagcacac cgttgggacg acccgagtca
      421 agcgggggat ggccgagatg ctcaagggcg gcgtcatcat ggacgtggtc accgccgagc
      481 aggccacgat cgccgaggac gccggcgcgg tggccgtcat ggccctggag cgggtgcccg
      541 cggacatccg ggcgcaaggg ggcgtgtcgc gcatgagcga ccccgacatg atcgacgcca
      601 tcatcgcggc ggtctcgatc cccgtcatgg ccaaggcccg gatcggccac ttcgtcgagg
      661 cgcaggtcct gcaggcgctc ggcgtggact atgtcgacga gtccgaggtc ctcaccccgg
      721 ctgactacgc caaccacatc gacaagtggg cgttcacggt gccgttcgtg tgcggggcga
      781 ccaatctcgg tgaggcgttg cggcggatca ccgagggggc ggccatgatc cgttccaagg
      841 gcgaggccgg gaccggcgac gtgtcccagg cgaccaccca catgcgtgag atccgcggcc
      901 agatcaaccg gctgcgctcg atgtcgagcg acgagctgta tgtcgccgcc aaggagctgc
      961 aggcgccgta tgacctcgtc gccgatgtcg cccgggccgg caagctgccc gtcgtcatgt
     1021 tcaccgcggg tggtatcgca acccccgccg acgcggccat gatgatgcag ctcggtgctg
     1081 agggcgtctt cgtcgggtcc gggatcttca aggcggacaa ccctgcccag cgggccgagg
     1141 ccatcgtcaa ggcgacgacc ttctacgacg accccgatgt catcgccaag gtctcgcgcg
     1201 ggctcggcga agccatggtc ggcatcaacg tcgaggacat cccagcgccc caccgcctcg
     1261 cccagcgcgg ctggtagccg aggtcaggtc accgccgccc gggactggaa ctcggggcgc
     1321 tcgtactcgc gactggccag gacctcggcc acgtgccggg cggccgtcag cacgtcgaca
     1381 tggctcaggt acaagggcgc gaacccgagc cgcacgatgt ccggcctgcg gaagtcgccg
     1441 atgacacccc gcgcgatgag cgcctggacg acggccagtc cctgctcgtg gcgcaggctc
     1501 acctgcgacc cgcgccgctc gtctgccgct ggtgtcacaa cgtcgacgtc agcgccgagc
     1561 ccgtcgaggc actcgcggaa gaacgaggtc agcgacaacg agcgggcacg caccgactcg
     1621 atcgacagcc cgtcgtatgc cgagagcgcg gcctccagcg ccagcaggct cagcagcggg
     1681 ggactgccga cccgggcgcg cgtgatcgag ggcgccggct cgtagagcgg ccccatctcg
     1741 aacggccgcg cgtgaccgtg ccagccgctc agcgggttgt ggaaggacgc gagatgacgc
     1801 tgcgcgacat acacgaaggc gggtgcaccc ggcccgccgt tgaggtactt gtacccgcag
     1861 ccgactgcga agtccacccc atgcgcgtcg agacccaccg gcaggacgcc ggccgagtga
     1921 cacaggtccc agctcgcgag ggccccgatg tcgtgcgcgg cctcggtgat cgccaggagg
     1981 tcccacagct cgcccgtgcg gtagtcgacc gaggagtatg acgcgagcgc cacccgggtg
     2041 ccgagctcgc ggagacgttc cggggcgcgg tccggagtgg ccagctcgat ggaccatccg
     2101 gcatacccgg cggcgctctg caggacgtag aggtcggtgg ggaacgaccc ggggtcgatg
     2161 agcacgatgt cgcgtcccgg ccgcatccgc gcggccgcga ggtagacctt ggcgaggttg
     2221 accgatgtcg aatcggtgac gacgacctgg ccgggagagg ccccgatgag cgccccgacg
     2281 aggtctccga cccgctccgg cgcgccccac cagtcgtgct cgttccagga ccggaccagc
     2341 cggtcgcccc actgctgtgt cacgaggcgc tggacaacct cgggcacggc cgcgggcagg
     2401 gcgccgagcg agtttccgtc gaggtagaca acgtccgctg gcagcgaaaa ggccgatcgg
     2461 cggtcggtcg cgtcgtggac ggcatcgagc tgcgcagccc gggcccccag agggccgggc
     2521 gcggcggtgg aggcgagaga cgaatgggtc acgcgtgaga ccgtatccga gcggtggact
     2581 atccgagcgg tgggcgtatc gtctcggctc gtgaccgtcc cccggagccc cgtcgtcggt
     2641 gtgctcgccg tccagggcga cgtccgtgag cacctgcgtg ccctcaccgc cctgggcgcc
     2701 cgagcgacca gcgtgcgacg gccccgtgag ctcgaccagg tcgacgccct cgtcatcccc
     2761 ggcggcgagt cgaccaccat ggacaagctc gtccgggcct tcggtctcga cgagcccctt
     2821 cgcaggcgga tcgccgaggg gatgccggtc tatggctcgt gcgccgggat gatcatgctc
     2881 gccgaccgga tcgctgacgc ccgacccgac cagcactgcc ttggtggcct cgatatcacc
     2941 gtgcggcgca acgccttcgg gcggcaggtc gactccttcg aggaggacct ggacttcacc
     3001 ggtctggccc acccggtcca cgccgtcttc atccgcgcgc cctgggtcga gcagttcggc
     3061 gccgccgtgg aggtcctcgc ccgcatcgag gcaggtcccg ccgcgggtag gatcgtcgcc
     3121 gttcgtcagg gcgcgttgct cgccacctcc ttccaccctg aggtcaccgg tgacacgcgt
     3181 gtgcacgccg agtttctgac gctggtccaa gccagcagat cgtgagggat cggtcatgag
     3241 cggccactcc aagtgggcga cgacgaagca caagaaggcc gtggtcgacg ccaggcgcgg
     3301 cgcgctcttc gccaagctca tcaagaacat cgaggtcgcg gcgcgcaccg gtgggggcga
     3361 ccctgatgcc aaccccaccc tctgggacgc gatctacaag gccaagaaga cctcggtccc
     3421 caacgacaac atcgaccggg cggtcaagcg gggcagcggg gtcgaggccg gcggcgccga
     3481 ctgggcgacg atcatgtacg agggctacgc ccccgggggg gtcgccgtgc tcgtcgagtg
     3541 cctcaccgac aaccgcaacc gagcggccgc ggcagtccgc accgcgctga cgcgccacgg
     3601 gggttcgctc gccgaccccg gcagcgtctc ctacctgttc gcgcgcaagg gtgtcgtcat
     3661 ggtgcccagg ggctcgctca cggaagacga cctgctcgag gtcgtcctcg atgccggcgc
     3721 agaggagatc aacgacatcg gtgaggcctt cgagatcgtg agcgagccgg gtgacttcgt
     3781 ggccgtccgt accgctcttc agggggccgg catcgactac gactcggcag aggcttcctt
     3841 catccccact gtggaggtcg agctcgatct cgatggtgcc cgcaagatgt tccgggtcat
     3901 cgaggccctc gaggacagcg atgacgtaca gaatgtctgg gccaacgggg acgtcagtga
     3961 caagatcc
//
LOCUS       DATAIX010000460         3193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_114595, whole genome shotgun sequence.
ACCESSION   DATAIX010000460 DATAIX010000000
VERSION     DATAIX010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114595"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..1070
                     /locus_tag="VHM65_06885"
                     /pseudo
     CDS             300..1070
                     /locus_tag="VHM65_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903157.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1285..2484
                     /locus_tag="VHM65_06890"
     CDS             1285..2484
                     /locus_tag="VHM65_06890"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2705467.1"
                     /translation="MEVFVGLDWGEQHHQVHALDGGSTTRLSFRVPHDRSGLERLRAA
                     LAGLGIPSEVGVAIERREGLLVDHLLSWGHPVYPVNPKVAARAREGYRAAPVKSDALD
                     AFALADLLRRQATSWRPLRPSSAVHAELQALVRDRERFVVEHRRFQHQLRAALETYYP
                     AATKLFSGLDRSITLAFLRRYPTPEPAERLTVHRLARFLARQGYSGRTAPEVLVERLR
                     THAGTPNRGVDAARARAMLALVDLLEHAGRVLDDYEAAIAGVLARHPDAALVASFPGT
                     GPITTATLVAEIGEDRSRFPSSGSLLAEAGLAPVTRQSGSSRRVGFRYAANHHLRAAW
                     GQWMLTAIRISPWTREAYDAARARGQSHSRAVRSVGARWGRILWRCWQDGQAYDPALD
                     RRVQLAA"
     gene            2494..2982
                     /locus_tag="VHM65_06895"
     CDS             2494..2982
                     /locus_tag="VHM65_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2705468.1"
                     /translation="MTEGVCRVHLTRNAQDLVPRSARSMVASAVRSVIEQPDGAAARA
                     QLDRVIDGLRGSAPRVADLLTDAEPDLLAHFAFPESHRRQIRSTNPLERLNKEIKRRT
                     AVVGIFPNRDAVIRLVGMILAEQDDEWQDGRRYFRPETMGALDGPPPSSEEVNPALLM
                     AS"
BASE COUNT          435 a         1162 c         1134 g          462 t
ORIGIN      
        1 ggtcatgtgc acagctcaac actccctcca ccatcaggtc atggtgtgtg cgctttcggt
       61 gccggatctg cttgccgcgt cggcttcaga cgactccggc ttccagcggg gccagaccgg
      121 caatcggccg gtagcgccga cgcgctcggt cagtcgagcg gctggcgtgg aggctggggg
      181 acctgccgcg ggtcgtgtcc ccagagttgg tgtaacagcg gatggccgtg aacgagaacg
      241 gccaccgcgt catcctcgcg tcgttggact tgcaaccgac gacacggagg aacaccacga
      301 tggccgagga caggatggcc gtgctcgaca ccctgcgcaa ggcgctcgcc agcggcgacg
      361 tcgacttcct gcgcgaaggg gtccgagtgc tcgcccaggc ggtgatggag accgaggtga
      421 ccgagctcac cggcgtgccc aggggtgagc gcgaccccga cgctcgcctg acccaccgca
      481 acggctaccg ggcacggcca tgggacacgc gggtcggcac gatcgagctc gccatccccc
      541 gggttcgcga cgggtcgtac ttcccgagcc tgctcgaccc gcgtcgacgg gccgagcggg
      601 ccctgctcgc ggtggtctgc gaggcgtacg tggcgggcgt ctcgacccgc cgggtcgacg
      661 acctcgtgcg ctcgctcggg atcgccggga tcagcaagag cgaggtgagc cggatgtgtg
      721 ccaccctcga cgccgaggtg gcggcgttcc ggacccgctc gctcgccggc gagcgctacc
      781 cgtacctgtg gctcgacgcc acgtatctca aggtgcgcga ggcgggccgg gtggtgtcga
      841 tggcggccct cgtcgcgacc ggggtcgccc gctcgggcga gcggcgcatc ctggctctcg
      901 agctcagccc cggtcacgac gaggggagcg cctggccccg cttcatccgc tcgctcgtcg
      961 agcgcggcct cgccggcgtg ctcctcgtca tctccgacga ccacgcgggg ctcgtcaaag
     1021 ccgtccgcga gcagctcctg ggtgcttcgt ggcagcgctc atcggtgcac agtgggctgg
     1081 gaccgagggt ccccgtctcg cacccgagcg gagagcgcct tggcagcggc gatcggtttc
     1141 agatcctccg gcagacccgc gcctccgctc ggggggcgag cctataccgg cccgagggag
     1201 atgtgcttcg agcaccgacg atgagcgtcc cgggtgggct gcgccgagcg gcgagacgag
     1261 gcgtcgaagc tggagggcac gaccgtggaa gtcttcgtcg ggctcgactg gggcgagcag
     1321 catcaccagg tccacgcgct cgacggcggg agtacgaccc ggctctcgtt ccgggtgccg
     1381 cacgatcggt ctgggctgga gcgcctccgg gccgcgctcg ccgggctcgg catcccatcc
     1441 gaggtcggcg tcgcgatcga gcggcgcgag gggttgctgg tcgaccacct gctctcttgg
     1501 ggccaccccg tctaccccgt caacccgaag gtggcggcac gtgcccgtga ggggtaccgg
     1561 gctgctccgg tcaagagcga cgcgctcgat gcgttcgcct tggccgacct ccttcggcgc
     1621 caggccacga gctggcggcc gctccgcccg tcgagcgcgg tgcatgccga gctccaggcg
     1681 ctcgtccgtg atcgcgagcg cttcgtcgtc gagcaccggc gcttccagca ccagctccgg
     1741 gccgccctcg agacctacta cccggcggcc acgaagctgt tcagcgggct cgatcgctcg
     1801 atcaccctcg ccttcctacg ccggtatccg acgcccgagc cggcggagcg cctgacggtc
     1861 caccgtttgg ctcggttcct cgcccgccag ggttactcgg gccggacggc gcccgaggtc
     1921 ctcgtcgagc ggctccggac ccatgccggc acgcccaacc gtggggtcga tgccgcccgt
     1981 gcccgggcga tgctcgccct cgtcgacctt ctcgaacacg ctggccgggt cctcgacgac
     2041 tacgaagccg cgatcgccgg cgtcctggcg cggcacccgg acgccgcgct ggtcgcgagc
     2101 ttccccggga cgggaccgat cacgaccgcg acgttggttg ccgagatcgg cgaggaccgg
     2161 tcacggttcc cctcgtccgg gagcctgctc gccgaggccg gcctggcgcc ggtcactcgc
     2221 cagtcgggat cgtcgcggcg ggtcggattc cgctacgcgg cgaaccacca cctccgcgcg
     2281 gcttggggcc agtggatgct gaccgccatc cggatctcgc cctggacgcg cgaggcctac
     2341 gacgccgccc gggctcgtgg ccagagccac agccgggcgg tccgctcggt cggtgcgcgt
     2401 tggggccgga tcctctggcg ctgctggcag gacgggcagg cctacgaccc tgccctcgac
     2461 cgacgggtac agctcgccgc ctgacggtcg gggttgacag agggagtctg ccgcgtccac
     2521 ctgacacgca acgcccagga cctcgtgccg cgctccgccc ggagcatggt cgcgagcgcg
     2581 gtccggtcgg tcatcgagca gcccgacggg gccgccgccc gggcgcagct cgaccgggtg
     2641 atcgacggcc tgcggggcag cgctccccgg gtggcggacc tgctcaccga cgccgagccc
     2701 gacctgctcg cccacttcgc gttccccgag tcccaccggc gccagatccg gagcacgaac
     2761 ccgctcgagc ggctcaacaa ggagataaag cggcggaccg cggtcgtcgg gatcttcccc
     2821 aaccgcgatg ccgtcatccg cctcgtcggg atgatcctcg cggagcagga cgatgagtgg
     2881 caggacggtc ggcgctactt ccggcccgag acgatggggg ccctcgatgg gccgccgccc
     2941 agctcagagg aggtgaaccc ggccctgctc atggcgagct gatcacccgg cgcgaagatg
     3001 acgtgctgct acaccacatc cgggacttga ccgggtccaa ccggccagct cacgacgggc
     3061 gaccgcgggg cgctcgccta cgccaaaccg cgagcgtgac gtcggtcgat ggtcacctgt
     3121 cgccccgctc ggataagacc tccttctggg acggtcggga tgacgcccgg gcaccgcacg
     3181 gatcaggcac gag
//
LOCUS       DATAIX010000461         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_279160, whole genome shotgun sequence.
ACCESSION   DATAIX010000461 DATAIX010000000
VERSION     DATAIX010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279160"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VHM65_06900"
     CDS             complement(<1..402)
                     /locus_tag="VHM65_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2705469.1"
                     /translation="MPQGPRDDVDSIVAAWVRERPDLDVGPLHVLSRVSRLARHLDLA
                     RGSAFAEHGLEGWEFDVLSALRRVGAPYQLSPGALVAQTLVTSGTMTNRVDRLEQRGY
                     VARSPDPTDRRGVIVTLTPRGAEAVDAAMADL"
     gene            497..1303
                     /locus_tag="VHM65_06905"
     CDS             497..1303
                     /locus_tag="VHM65_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2705470.1"
                     /translation="MWDPAQYALFAGHRGRPFGDLLARVEAPSPRLVVDLGCGDGLLT
                     RGLAQRWPQARVVGIDSSPQMLAAARADDTQGRVEWLLERVETWDPAGLGAGVDVIVT
                     NATLQWVPGHLGLIPRWVDVLEPGGWFAMQVPANYDAPSHRLMREVASRHPRAADLAP
                     GLRRAAAVAEPAAYLELLSSLGLGTDVWETTYLHVLDPEGTLRSPVLEWVRATGLRPV
                     LDVLTDRDELAAFLEDYTAALDEAYPRRAHGVVLPFRRVFAVARREPVAS"
BASE COUNT          198 a          542 c          548 g          228 t
ORIGIN      
        1 caggtcggcc atggcggcgt cgacggcctc ggccccacgc ggcgtcagcg tcacgatgac
       61 gccgcggcgg tcggtcgggt cgggactgcg cgcgacatac ccccgctgtt cgagccggtc
      121 gacccggttg gtcatggtgc cggaggtgac cagggtctgg gccacgagcg ccccagggga
      181 gagctggtat ggcgcgccga ctcgtcgtag tgccgacagg acgtcgaact cccagccctc
      241 gaggccgtgc tcggcgaagg ccgagccgcg cgcgaggtcg aggtggcggg cgagtcggga
      301 cacccgggac aggacgtgca gggggccgac gtcgaggtcg ggacgctccc ggacccaggc
      361 cgccacgatg ctgtcgacgt cgtctcgcgg tccttggggc acgcgtcaag aatactctcc
      421 tcaagtattt tgacatcgag ataaactggg accgaacctt ggagcagatc ccttcgtcgc
      481 gggaaggact gacatcgtgt gggacccggc ccagtacgcc ctgttcgccg gtcatcgcgg
      541 acggcccttc ggcgacctgc ttgcccgcgt cgaggccccg agcccccggc tcgttgtcga
      601 cctcggctgt ggcgacggcc tgctcacccg ggggctggcc cagcgctggc cgcaggcccg
      661 ggtcgtcggg atcgactcgt cgccgcagat gctcgccgcg gcccgggccg acgacacgca
      721 ggggcgggtc gagtggctcc tggagcgggt cgagacatgg gacccggccg gcctcggcgc
      781 gggggtcgac gtcatcgtca ctaacgcaac cctgcagtgg gtgcccggcc atctcggcct
      841 catcccgcgc tgggtcgacg tcctcgagcc cggcggctgg tttgcgatgc aggtcccggc
      901 caactacgac gcgccctcgc accggctcat gcgcgaggtg gcgagccggc acccgcgggc
      961 cgccgacctg gcccccggac tgcggcgagc cgccgcggtg gccgagcccg cggcatacct
     1021 ggagctgttg tccagcctcg gcctggggac cgacgtgtgg gagacgacct acctgcacgt
     1081 cctcgacccc gaggggaccc tgcgctcgcc ggtcctcgaa tgggtccggg cgacggggct
     1141 gcgaccagtc ctcgacgtgc tgaccgaccg ggacgagctg gctgccttcc tcgaggacta
     1201 caccgctgcg ctcgacgagg cctacccgcg tcgggcgcac ggcgtcgtcc tgcccttccg
     1261 gcgcgttttc gccgtcgcgc ggcgcgagcc cgtggcatcc tgaccgccct ggcggctccc
     1321 gacgcggcgc cataccggcc actcacctgt cgacgagccc accgtcgacg gtcacccggc
     1381 gacggaccct tggcgagcgt cgggcttacc tgcatcggct ctggcctgca agtaggtgtt
     1441 tcggttggaa tgagggccaa cgctcggtgc tgggggcgcg cctgatcgtg cttcctgacc
     1501 ggatagctgc cagtac
//
LOCUS       DATAIX010000462         4132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_249286, whole genome shotgun sequence.
ACCESSION   DATAIX010000462 DATAIX010000000
VERSION     DATAIX010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249286"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1441)
                     /locus_tag="VHM65_06910"
     CDS             complement(<1..1441)
                     /locus_tag="VHM65_06910"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX2705471.1"
                     /translation="MRAPLSWLGEYVDIAPGTTGAQVAASLVSVGLEEEDLHGGDITG
                     PLVVGRVLDLVDEPQKNGKTIRWCHVDVGSHGQRVTDGSAQEIVCGAHNFAAGDLVVV
                     VLPGAVLPGGLAVSARRTYGHVSHGMICSAAELGLGEDHSGIIVLQDYLGADAATSLQ
                     PGDDAIALLGLADEVIEVNVTPDRGYCFSLRGIAREYWHATGSRPGAFRDPVAIGTPV
                     PSTNGYAVELADEAPLGGAEGCDRYVARVVRGIDPAASSPAWLERRLTQMGMRPISLA
                     VDVTNYVMLAVGQPLHAFDVATLNGPIVVRRAKPGETLTTLDDVERRLDSQDLLITDS
                     GTVPLAIAGVMGGATSEVTSATTDVLVEAAHFDPTTTARSSRRHKLVTEASRRFERGV
                     DPAMTAEAAQLVVDLLVRYGGGIPDPGVTDVDHTSPRAPYRLDVTGPSRLIGVDYPRE
                     RVVEILRDLGCAVEDAGPGSSTVSVTPPPW"
     gene            complement(1443..2531)
                     /gene="pheS"
                     /locus_tag="VHM65_06915"
     CDS             complement(1443..2531)
                     /gene="pheS"
                     /locus_tag="VHM65_06915"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX2705472.1"
                     /translation="MPPGPGASPLAPASLQRAVDAALAATAAAGSLDALKAARIAHQG
                     EKSPLALANRGIGALPPSEKAAAGQRVGAARGQVAAALTARQAELEAERDERILVEEA
                     VDVTARVSRRAIGARHPIELVAERLADAFVGMGWEVAEGPEVEGEWFNFDALNLGPDH
                     PARQMQDTFFVDPPDSGLVLRTHTSPVQARTMLTRTPPIYVVCPGKVFRTDDLDATHT
                     PVFHQIEALAIDTGLTMAHLRGTLDAFVRIMFGEDTRTRVRASYFPFTEPSMETDFVC
                     FACRGAGSTDDGQCRTCGGSGWIELGGSGMVNRKVLAACGIDPDVYSGFAFGLGIERA
                     LMLRHGVQSMHEMVEGDVRFSAQFGMVV"
     gene            complement(2644..3750)
                     /locus_tag="VHM65_06920"
     CDS             complement(2644..3750)
                     /locus_tag="VHM65_06920"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX2705473.1"
                     /translation="MLQSSTTTAVRAPMPVPMAVMGLFEMLPDGLVVIDKRGIIRYLN
                     SRAARILGTSSEDLLGREVRVALPLRTADGCSWWDCTNPWEGLHTRTGHRERLLSVDG
                     EREVLVTAQYIRPARGQPVERIIMSLRDAAARQRAEADHAALISTVAHELRSPLTSVK
                     GFSATLLRRWDRFTDDQKRFMLQTIEADADRVTRLITELLDVSRIDAGRLTVHRQPVD
                     LVATLDRHVSRFVASGLPADRFVVDVAPDLPEVWADPDRLDQILLNLIENALRHGEGT
                     VTLGSAIDPGHPHIDRPPAVVITVGDEGQGIAEEDYPLIFSRFWHGSRRGSTGLGLYV
                     VRGLVEAHGGQASVGRAPGGGAEFRFTLPSGIPS"
     gene            complement(3761..>4132)
                     /locus_tag="VHM65_06925"
     CDS             complement(3761..>4132)
                     /locus_tag="VHM65_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX2705474.1"
                     /translation="VLVSDASVDVYNPRVVRASAGSVFHVPIVTGLPVETILGEMRHA
                     GLRLFAADGAAAESLDDADLTSAHAWVMGNEAWGLPESTRDACDAVVRVPIYGQAESL
                     NLAMATTICLYASARQQRTHR"
BASE COUNT          594 a         1408 c         1559 g          571 t
ORIGIN      
        1 gccagggcgg tggggtgacc gacacggtgc tgctgcccgg gccggcatcc tcgaccgcgc
       61 agcccaggtc gcgcaggatc tcgacgaccc gctcgcgcgg gtagtcaacg ccgatgagcc
      121 gggagggacc ggtgacatcg agccggtatg gcgcgcgcgg gctcgtgtgg tcgacgtccg
      181 tcacgcccgg gtcagggatg ccgccgccgt agcggacgag caggtcaacg acgagctggg
      241 ccgcctccgc cgtcatggcc gggtcgactc cgcgctcaaa ccgcctggac gcctcggtga
      301 cgagcttgtg ccgccgcgac gaccgggcag tcgtcgtcgg gtcgaagtgc gccgcctcga
      361 cgaggacgtc ggtggttgcg gacgtcacct ccgaggtggc cccgcccatg acgccggcga
      421 tggcgagcgg gacggtgccg ctgtcggtga tgagcaggtc ctgtgagtcg agccggcgct
      481 cgacgtcgtc cagtgtcgtc agggtctcgc ccggcttggc ccggcgcacc acgatgggtc
      541 cgttcagggt cgccacgtcg aaggcgtgca gcggctgccc gacagcgagc atgacgtagt
      601 tggtcacgtc gacggccagg gagatcgggc gcatccccat ctgggtgagg cgccgttcga
      661 gccacgccgg ggacgacgcg gcggggtcga tgccgcggac cacgcgggcg acataccggt
      721 cgcagccctc ggcaccgccc agcggcgcct cgtcggcgag ctcgacggca tacccgtttg
      781 tgctgggaac gggtgtcccg atggcgacgg ggtcgcggaa ggcgcccggg cgcgagccgg
      841 tggcgtgcca gtactcgcgg gcgatgccgc gcagggagaa gcagtagcca cggtcgggcg
      901 tcacattgac ctcgatgacc tcgtcggcca ggccgagcag ggcgatcgcg tcgtcaccgg
      961 gctgcaggct cgtggcggcg tcggcaccca ggtagtcctg caggacgatg atgccgctgt
     1021 ggtcctcgcc gaggccgagc tcggcggccg agcagatcat cccgtgggag acatgcccgt
     1081 aggtcctgcg ggccgagacc gcgaggccgc ccggcaggac ggccccgggc aggacgacga
     1141 cgacgaggtc cccggcggcg aagttgtggg caccgcacac gatctcctgg gcactgccgt
     1201 cggtgacccg ctggccgtgg ctgcccacgt cgacgtggca ccagcggatc gtcttgccgt
     1261 tcttctgcgg ctcgtcgacg aggtcgagga cccggccgac gacgagcggg ccggtgatgt
     1321 cgccgccgtg gaggtcctcc tcctcgagac cgacgctgac cagggacgcg gcgacctgcg
     1381 cgccggtcgt ccctggcgcg atgtcgacgt actcgcccag ccaggacagg ggggcgcgca
     1441 tctcagacca ccatcccgaa ctgggccgag aagcggacgt cgccctcgac catctcgtgc
     1501 atgctctgga cgccgtggcg cagcatgagc gcccgctcga tgccgagccc gaacgcgaag
     1561 ccgctgtaga cgtcggggtc gatcccgcag gccgcgagga ccttgcggtt gaccatgccg
     1621 gagccaccga gctcgatcca gcccgaaccg ccacaggtgc ggcactggcc gtcgtccgtc
     1681 gacccggccc cacggcaggc gaagcagacg aagtcggtct ccatgctcgg ctcggtgaac
     1741 gggaagtagg acgcccgcac cctcgtgcgg gtgtcctcgc cgaacatgat ccgcacgaac
     1801 gcgtcgagtg tcccgcgcag gtgcgccatc gtcaggcccg tgtcgatcgc gagggcctcg
     1861 atctggtgga agaccggggt gtgggtcgcg tcgaggtcgt cggtccggaa gaccttgccc
     1921 ggacagacga cgtagatcgg cggggtgcgg gtcagcatcg tgcgcgcctg gaccggcgag
     1981 gtgtgggtgc gcaggacgag cccggagtcg ggtgggtcga cgaagaacgt gtcctgcatc
     2041 tggcgggcgg ggtggtcggg gccgaggttg agcgcgtcga agttgaacca ctccccctcg
     2101 acctcggggc cctcggcgac ctcccagccc atcccgacga acgcgtcggc cagccgctcg
     2161 gcgacgagct cgatcgggtg ccgggcgccg atggcgcgcc ggctcacccg ggccgtgacg
     2221 tcgacggcct cctcgacgag gatccgttcg tcacgctcgg cctcgagctc ggcctgccgg
     2281 gcggtcaagg ccgccgcgac ctggccccgg gcggcaccga cgcgctggcc ggccgcggcc
     2341 ttctcggacg ggggcagggc accgattccg cggttggcca gcgcgagcgg gctcttctcg
     2401 ccctggtggg cgatccgcgc ggccttgagc gcgtcgaggc tcccggccgc ggcagtcgcg
     2461 gccagcgccg cgtcgaccgc tcgctggagg ctggccgggg cgagtgggct cgcaccgggg
     2521 ccgggcggca tggtaggtcc agacatcact gtcttccatt tcgggtcgcg ccagagtcta
     2581 ggggtgagcc ggaggcgact tcgggcggcg accggagcgt gggaaccacg agcgtgactc
     2641 cggtcaggag gggatgcccg aaggcagggt gaatcggaac tccgcgccgc cgccgggcgc
     2701 gcggccgacc gatgcctgcc cgccgtgggc ctcgacaagc ccgcgcacga catacaggcc
     2761 caggcctgtg ctcccgcgcc gggacccgtg ccagaagcgg gagaagatca gcgggtagtc
     2821 ctcctcggcg atgccctggc cctcgtcgcc caccgtgatg acgaccgccg gaggccggtc
     2881 gatatgtggg tggcccgggt cgatcgccga gcccagggtc accgtgccct caccatggcg
     2941 cagggcgttc tcgatgaggt tgagcaggat ctggtcgagc cggtccgggt ccgcccacac
     3001 ctcgggcaga tccggggcca cgtccacgac gaaccggtcc gccggcaggc ccgacgcgac
     3061 gaaacgggac acgtgccggt cgagcgtggc caccaggtcg acgggctggc ggtgcacggt
     3121 gaggcgaccg gcatcgatcc gtgagacgtc gagcagctcg gtgatgagcc gggtcacccg
     3181 gtcggcgtcg gcctcgatcg tctggagcat gaaccgcttc tggtcgtcgg tgaaccggtc
     3241 ccagcggcgc agcagggtcg cgctgaaccc cttgaccgag gtcagtggcg agcgcagctc
     3301 gtgggcgacc gtggagatga gcgcggcgtg gtcggcctcg gcgcgctggc gggccgcggc
     3361 atcgcgcagc gacatgatga tccgctccac tggttggccc cgcgcggggc ggatgtactg
     3421 cgcggtgacg agcacctcac gttcgccgtc gaccgagagc aggcgctcgc gatgcccggt
     3481 tcgggtatgc agcccctccc acgggttcgt gcagtcccac cacgagcagc cgtcggccgt
     3541 ccgcaacggc agcgcaaccc ggacctcgcg cccgagcagg tcctcggaac ttgtgccgag
     3601 gattcgggca gcccgggagt tgaggtagcg gatgatcccc cgcttgtcga tgacgaccag
     3661 cccgtccggc agcatctcga acagccccat gacggccatg ggcacaggca tcggcgcgcg
     3721 cacggctgtc gtggtggagg actggagcat tggtaggagc ctaccgatgg gtccgctgct
     3781 ggcgggctga ggcgtagagg cagatcgtcg tggccatggc gaggttgagc gactcggcct
     3841 ggccgtagat cggcacccgg accacggcgt cgcacgcgtc gcgggtcgac tcgggcagac
     3901 cccacgcctc gttgcccatg acccaggcgt gggcgctggt caggtcggcg tcgtcgaggc
     3961 tctcggcagc ggcgccgtcg gcggcgaaca ggcgcagccc ggcgtgacgc atctcaccga
     4021 ggatggtctc gacgggcaga ccggtgacga tcggaacatg aaagaccgat ccggcgctcg
     4081 cgcggaccac cctggggttg tagacgtcga cgctcgcgtc gctgaccagc ac
//
LOCUS       DATAIX010000463         4425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_174438, whole genome shotgun sequence.
ACCESSION   DATAIX010000463 DATAIX010000000
VERSION     DATAIX010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174438"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VHM65_06930"
     CDS             <1..298
                     /locus_tag="VHM65_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX2705475.1"
                     /translation="SQLVRNIDLVRSHLNPGLRVSTILLTMYDARTRLSAQVAEEVRS
                     HFPDEVLRSTIPRAVRVSEAPSFGQTVMTYDPSSAGALSYLEAASELAARGAAR"
     gene            340..1431
                     /locus_tag="VHM65_06935"
     CDS             340..1431
                     /locus_tag="VHM65_06935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX2705476.1"
                     /translation="MNEKRRALGRGLGALIPSAPASGESARPVDLFFRDQDRPTSAQA
                     APTPQDFDGAEAGQGLPGLAPVPGATFAEVGIDDIRPNPRQPREAFDTDELAELVHSI
                     REIGVLQPVVVRPIPEEELAAASRSDPSGAQRAGDPAVGAGPWVPVPRYELIMGERRW
                     RASREAGKATIPAIIKDTHDDNLLRDALLENLHRSQLNALEEAAAYQQLLDDFGCSHE
                     QLASRIGRSRPQISNTLRLLKLPPPVARRVAAGVLSAGHARALLGLAEAAAMERLAQR
                     IVAEGLSVRSVEEIVALGGDAPVKSRKPRAGRRHPQLDDYVGILQDRFDTRVQIALGQ
                     RRGRVTIEFASVEDLNRIIDLMGVRRLGD"
     gene            complement(1500..1826)
                     /gene="trxA"
                     /locus_tag="VHM65_06940"
     CDS             complement(1500..1826)
                     /gene="trxA"
                     /locus_tag="VHM65_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX2705477.1"
                     /translation="MGASQATSDATFDQDVLQSDRPVLVDFWADWCGPCRAMEPVIDE
                     IALQHGDKLKVVKIDTEQNPAIPLRYGVVGLPTFNVYVAGELVKSFSGAKPKHWLLRE
                     LAEFID"
     gene            complement(1925..2917)
                     /gene="trxB"
                     /locus_tag="VHM65_06945"
     CDS             complement(1925..2917)
                     /gene="trxB"
                     /locus_tag="VHM65_06945"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX2705478.1"
                     /translation="MSSDVRTVIIIGSGPAGYTAAVYAARANLHPLVFEGSVTAGGAL
                     MNTTEVENFPGFRHGILGPDLMDEMRAQAERFGAELVRDDVLSVSLVGDIKEVVDSAG
                     TTHRARAVILAMGSAYRELGLADEKRLSGRGVSWCATCDGFFFRDQDIAVAGGGDSAI
                     EEATFLTKFARSVTIIHRRDALRASKIMADRAMADEKIRFTWNSTVEAIHGATTLTGV
                     TLRDTVTGQTSELAVTGLFVAIGHDPRSELVKGVVDLDAAGYVLAEGRSTRTSVPGVF
                     ACGDLVDHTYRQAVTAAGSGCAAALDAERYLAHLSDPTTAGSRVPAIASGSAAR"
     gene            complement(3100..3861)
                     /locus_tag="VHM65_06950"
     CDS             complement(3100..3861)
                     /locus_tag="VHM65_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705479.1"
                     /translation="MRMPPPSASSEDDDPTGVRAILQAAPDPGPMPSEVMARIQATLV
                     QEQARRALLVPTAPSTAPGPGAGQVIPLVGMSRRRRSRRGSRFGSLGGAAAAILLLAV
                     GLAVLAPNLLRSLGASNGSTSAAGGAEVARDSAATGPLVVLRASGTTFTASGLVEHAR
                     RLSAESSPQRDESLTSTGSSPWGSDQVLDCLDALGVRVSPELTVLSDTGSYDGAPAAV
                     LVVGRSAAPTRTAYLVRLPCSSANTGLITSRPLAP"
     gene            complement(4123..>4425)
                     /locus_tag="VHM65_06955"
     CDS             complement(4123..>4425)
                     /locus_tag="VHM65_06955"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX2705480.1"
                     /translation="CLDRLRRDKPTRPLPAIELAARGDAHASTEVRLDIHLALGALPE
                     GQRLALVLVDMHGLSIAEAAEILQVAEGTVKSRCSRGRAAMAQLLGLSRDGEVPHD"
BASE COUNT          651 a         1553 c         1604 g          617 t
ORIGIN      
        1 gtcccagctc gtgcgcaaca tcgacctggt ccgcagccac ctgaaccccg gcctgcgcgt
       61 ctcgacgatc ctgctcacga tgtatgacgc ccgcacccgc ctctccgcgc aggtcgccga
      121 agaggtgcgg tcccacttcc ccgacgaggt gctgcgcagc acgatcccgc gtgcagtccg
      181 cgtgtccgag gcaccgagct tcgggcagac ggtgatgacg tacgacccgt cgagcgcggg
      241 cgcgctgtcg tacttggaag ccgcgtcgga gctggccgcc cggggcgccg ccagatagcc
      301 gcctcaccgc ctgttggccg acaacgaatc gagcagacca tgaacgagaa gcgacgagca
      361 ctgggccggg gactcggcgc tctcatcccc agcgcgccgg cctcagggga aagcgccagg
      421 ccggtcgatc tcttcttccg ggaccaggac cgtccgacga gcgcccaggc tgcacccacg
      481 ccccaggact tcgacggggc ggaggccggc cagggcctcc ccgggctggc gccggtgccg
      541 ggagccacct tcgcggaggt cggcatcgac gacatccggc ccaacccgcg ccagccccgc
      601 gaggccttcg ataccgacga gctggccgag ctcgtccact cgatccgcga gatcggcgtc
      661 ctacagccgg ttgtggtgcg cccgatcccc gaggaggagc tggccgcagc ctcgcggtcg
      721 gacccttccg gggcgcagcg ggctggtgac cctgccgtcg gcgccggtcc ctgggtgccg
      781 gtccctcgct atgagctgat catgggcgag cgccggtggc gggccagccg ggaggccggc
      841 aaggcaacga tccccgccat catcaaggac acccacgacg acaacctgct ccgggacgcg
      901 ctcctggaga acctgcaccg cagccagctc aacgccctgg aagaggcggc cgcctatcag
      961 cagctgctcg acgacttcgg ctgctcgcac gagcagctgg cctcccgcat cggccgctcc
     1021 cggccgcaga tctccaacac gctgcgcctg ctcaagctgc cgccaccagt ggcccggcgc
     1081 gtggctgccg gcgtgctcag cgcggggcac gcgcgggccc tgctcggcct cgccgaggca
     1141 gccgcgatgg agcgcctcgc gcagcgcatc gtcgccgaag gcctgtcggt gcgaagtgtc
     1201 gaggagatcg tcgccctggg cggcgatgcc ccggtgaagt cccgtaagcc ccgggccggt
     1261 cgacgccacc cccagctgga cgactacgtg ggcatcctgc aggaccgctt cgacacccgc
     1321 gtccagatcg ctctgggaca gcgccgcggc agggtcacga tcgagttcgc ctcggtcgag
     1381 gatctcaacc gcatcatcga tctcatgggc gtgcgccgcc tcggggactg acggggacgg
     1441 cgctgcgggc ccgagccagg cggtccgagg gagccgggcc cgtcatcccg gcctggccgt
     1501 cagtcgatga actcggcgag ctcgcgcagg agccagtgct tgggcttggc ccccgagaag
     1561 ctcttgacga gctctccggc gacgtagacg ttgaaggtcg gcaggccgac cacgccatag
     1621 cgcaggggga tggcggggtt ctgctcggtg tcgatcttga ccaccttgag cttgtcaccg
     1681 tgctgaaggg cgatctcatc gatgacgggc tccatcgcgc ggcacgggcc gcaccagtcg
     1741 gcccagaagt cgacgagcac gggccggtcg ctctggagca cgtcctggtc gaaggtcgcg
     1801 tcggatgttg cctgggatgc tcccacagtg ggggtccttc ctcggggtgc ctggacgagc
     1861 ggggtgcgtg gacgagcggg gtgcgtggac gaacggggtg atgaccacgg tgcgggccgg
     1921 ccggtcagcg ggcggcggac cccgacgcga tcgccggtac ccgggatcct gcggtggtgg
     1981 ggtcggacag gtgggcgagg tagcgctcgg cgtcgagggc cgccgcgcag cccgatcccg
     2041 cggcggtgac cgcctggcgg taggtgtggt cgacaagatc gccgcaggca aagacgccgg
     2101 gcacggaggt ccgggtggac cggccctcgg cgagcacgta gcccgcggcg tcgaggtcga
     2161 cgacgccctt gacgagctcg cttcggggat cgtggccgat cgcgacgaag aggccggtca
     2221 cggcgagctc gctggtctga ccggtgaccg tgtcacgcag ggtcaccccg gtgagtgtgg
     2281 tcgccccatg gatcgcctcg acggtactgt tccacgtgaa acggatcttc tcatcggcca
     2341 tagcccggtc agccatgatc ttgctggctc gcagcgcgtc gcgacggtgg atgatcgtca
     2401 ccgagcgggc gaacttcgtc aggaaggtcg cttcctcgat cgccgagtcg ccaccgcccg
     2461 cgacggcaat gtcctggtcg cggaagaaga acccgtcgca ggtggcgcac cacgagacgc
     2521 cgcgcccgga cagcctcttc tcgtccgcaa gaccgagctc gcggtaggcc gaccccatcg
     2581 ccaggatgac ggcccgggcg cggtgagtcg tcccggccga gtcgacgacc tccttgatgt
     2641 cgcccaccag ggacacggac aggacgtcgt cccggacgag ctcagccccg aagcgctcgg
     2701 cctgggcgcg catctcgtcc atgaggtccg gccccaggat gccgtggcgg aatcccggga
     2761 agttctcgac ctcggtcgtg ttcatcagcg caccaccggc cgtgacgctg ccttcgaaca
     2821 cgagcgggtg caggttggcg cgggcggcat acacggccgc ggtgtatccg gccggtcctg
     2881 acccgatgat gatgacggta cgaacgtccg agctcacagc aggttccctc gactatccct
     2941 tgtggtcgac agcatacgtg ggccccacat catgtgattg accgtgaggg cggccgtccc
     3001 tgacatcaac cgatcctagg gccatgattg ttcctgccgc gcctccccca ggccccgggg
     3061 tcgacgtgcc ggcggctcgt ggagtcaggg agcgtggagt cagggagcga ggggccggga
     3121 cgtgatgagg ccggtgttgg cgctgctgca cggcaggcgc acgaggtagg ccgttcgcgt
     3181 cggggcagcc gaccgcccga cgacaaggac ggccgccggc gctccgtcat acgagccggt
     3241 atccgacagg acggtgagtt cggggctcac cctgaccccg agagcgtcga ggcagtccag
     3301 gacctggtcg ctcccccagg gcgacgaccc ggtcgacgtg agtgactcgt cccgttgcgg
     3361 cgaggactcg gcactgagcc ggcgagcgtg ctcgacgaga cctgacgcgg tgaacgtcgt
     3421 cccggaggcg cgcagcacga cgagcggacc ggtcgcggcg gagtcccgcg ccacctcggc
     3481 gccgcccgcc gcgctggtcg acccgttgct tgcgcccagc gagcggagca ggttcggggc
     3541 gaggactgcc agcccgacgg cgagcaggag gatggcggcc gccgcgccac cgagggaccc
     3601 gaaccgggac ccccgccggg accggcgccg ccgcgacatc ccgaccaacg ggatgacctg
     3661 gcccgcgccg ggtccgggcg ctgtgctggg agcggtcggc accagcaggg cccggcgggc
     3721 ctgctcctga acgagggtgg cctggatgcg ggccatcacc tcgctcggca tcgggcccgg
     3781 gtcgggagca gcctggagta tggcgcggac gcccgtggga tcgtcgtcct cggagctggc
     3841 tgacggaggg ggcattctca tgcggtcctc ctcccggtgc cgtcgacagg ccggtcggcg
     3901 tgcccgctgc tgagcgcgcc cggactggtt cggggccaca gcgcgacgct gccgaggacc
     3961 gtgcgccccg ggcgcctcgt ccaccatgac catgtatgag acgagcggtg cctgggatcg
     4021 gttccgagcg ggtccgatcc acaccgcagg cgacggatgg tgccacaacc ggcccgaggg
     4081 cgggtcgtcc ttcgcccccg gcgttcggga gcttcgcgca gctcagtcgt gagggacctc
     4141 cccgtcccga gagagcccga ggagctgcgc catcgcggcc cgaccccggg aacatctgga
     4201 cttcaccgtg ccctcggcaa cctgcaggat ctcggcggcc tcggcgatgc tgagcccgtg
     4261 catgtcgacg agcacaagag ccagccgctg gccctcgggg agcgcgccaa gggccaggtg
     4321 gatgtcgagc cgcacctccg tgctggcgtg ggcgtcaccg cgcgcggcca gctcgatcgc
     4381 gggcagcggg cgggtgggtt tgtccctgcg cagccggtcg aggca
//
LOCUS       DATAIX010000464         3518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_279250, whole genome shotgun sequence.
ACCESSION   DATAIX010000464 DATAIX010000000
VERSION     DATAIX010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279250"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(375..566)
                     /locus_tag="VHM65_06960"
     CDS             complement(375..566)
                     /locus_tag="VHM65_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705481.1"
                     /translation="MRGGLFETVWIPLISVKALRLGPLRIQRCPVHRRVEFVQLVDPA
                     TLTDADRAAAARYPADLIP"
     gene            complement(590..727)
                     /locus_tag="VHM65_06965"
     CDS             complement(590..727)
                     /locus_tag="VHM65_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705482.1"
                     /translation="MRTRSRALVGKGHRVSEDGTPITSSAPQKGARGSGPHFAQVLDL
                     W"
     gene            919..1248
                     /locus_tag="VHM65_06970"
     CDS             919..1248
                     /locus_tag="VHM65_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705483.1"
                     /translation="MCHYRFALHPRADAAAFEALMSARSGEDTLQLTRVTSGFSERLL
                     EVRRPADADEKSHHPGPQYVWEVTVNLVSGDHYDFAASADRVQEVAADLATLVSVESF
                     RTIETVA"
     gene            complement(1253..2026)
                     /locus_tag="VHM65_06975"
     CDS             complement(1253..2026)
                     /locus_tag="VHM65_06975"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2705484.1"
                     /translation="MSFAMSRAAYDRYMGRYSDQLASAFLSFAGVTPGMRALDVGCGP
                     GALTEALARRLGAAYVAAADPSESFVTACADRVPGADVRQGVAEGLPWPDATYDVVVS
                     QLVMNFLADADGGLEQMCRVVRPGGTVACCTWDYARGMVMLRTFWDAAFELDEAAPDE
                     GRLMRYCSEGELAGLWERHGVAEVETSTIDVTAEYAGFEDYWIPFTLGVGPGGSYLAS
                     LDAERQEQLRASCYRRLGAPGGEFTLPARAVAVRGRRPG"
     gene            complement(2188..3081)
                     /locus_tag="VHM65_06980"
     CDS             complement(2188..3081)
                     /locus_tag="VHM65_06980"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX2705485.1"
                     /translation="MSVDTEALRWFQQVADGVTVTEVADIHLVSQPGVSRALARLEDE
                     VGTPLLGRSGRVLRLTHAGSAFKRHVDALINNLDDGLAAVDQLVDPERGIVSVAFQLS
                     LGLWLVPELVSSFRAEHPGVHFVLSQVRDELLSPVLAGGRVDLEITARRPVGPQVRWR
                     RLVSEPLFIGVPAGHPLAGRSEITLAQVSAERFVALRLASLLRQQSHVLCAAAGFEPQ
                     IAVEADDLPTLRAFVVAGLGVAIVPAMGHDPAVAGPGAVRLVPITDPEAVRDVGLVWS
                     TERRLLPAAELFRRHVLSTAE"
     gene            3152..3424
                     /locus_tag="VHM65_06985"
     CDS             3152..3424
                     /locus_tag="VHM65_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705486.1"
                     /translation="MNQIQQRIHRAVSTSGIAPRLAKVREELAARQAQREARRALERE
                     IAGYTSVSDLADLQAALGRSGTEADEVREILQRHQSTLDHRRLRAC"
BASE COUNT          574 a         1311 c         1177 g          456 t
ORIGIN      
        1 ccgtgaatac cggaccggta cctaatgcgg atacgcaggg ggccggccgg atttcgcgcc
       61 ccgtggtttg ggcggtgccg tcgacatcgg gaacgtggtc cgccgtgcgg ggtttggccg
      121 tcgtgcccgt gccggcgacg cggttctgcc gggcgccgcc gcggggcacg ccccggcctg
      181 aggcctcgcc ctcccacgat cgacaggttc ggcgccgggc gaggatgccg acggacaagg
      241 tcggcggtgg ccgcaggggc ttggccactc gggactgccg ctactgcctc cggacaggac
      301 atgatctcga gcctcccgat cggacacccc agatcgtcgc gcgcgatcac cccagcgaac
      361 gcactccgac aagcctacgg aatgaggtca gccgggtagc gcgctgccgc ggcacggtca
      421 gcatcggtca gcgtcgccgg gtcgaccagc tggacgaact ccacccggcg gtgcacgggg
      481 caccgctgga tccgcagtgg acccagccgg agcgccttga cggagatcag cggaatccag
      541 acggtctcga acagcccgcc cctcatgcac cggacgatca ccacaggcat caccacaggt
      601 ccagcacctg cgcgaagtgc ggacccgagc ctcttgcgcc cttctgcgga gccgagctcg
      661 tgatcggggt gccgtcctcg ctcacccgat gccccttgcc cacgagcgca cgactcctcg
      721 tgcgcacatt catgctatcg gctcatccac tcccaggacg gatcagcccc gacgctggct
      781 gcccgaccag cagattaccg tcgccctcgg cctgacgtcc tggcgttgac acgccgcgcg
      841 ggagacacgc acactcaggg tgcctgacct cacgtcggcg tccgtccctg cgcacagaag
      901 aggggcacga tgaacgcgat gtgccactac cgattcgctc tccacccccg cgccgatgcc
      961 gccgcattcg aagcgctcat gtcggcccgg tccggcgagg acaccctaca gctcacccgg
     1021 gtgaccagcg gcttcagcga gcgtctgctc gaggtccgcc gacccgccga cgccgacgag
     1081 aagtcccacc acccgggccc gcagtacgtg tgggaggtca cggtgaacct cgtcagcggc
     1141 gaccattacg acttcgccgc gagcgccgac cgggtgcaag aggtggcggc ggacctggcc
     1201 acgctcgtct cggtggagtc cttccgcacg atcgagaccg tcgcctgatg gctcagcctg
     1261 gtcggcggcc gcgcacggcg acggcgcgag cgggcagagt gaactctccc cccggagcac
     1321 cgagtcgacg gtaacagctg gcccgtagtt gctcttgtcg ctcggcgtcc agcgatgcca
     1381 agtacgaccc gccaggtccg actcccagcg tgaacgggat ccagtagtcc tcgaagccgg
     1441 cgtactcggc cgtcacgtcg atggtgctcg tctcgacctc ggccaccccg tgacgctccc
     1501 acaggccggc cagctcgcct tcactgcagt agcgcatgag ccgaccctcg tctggcgcgg
     1561 cctcgtcgag ctcgaacgcc gcatcccaga acgtgcggag catcaccatg ccacgggcgt
     1621 agtcccacgt gcagcacgcg actgtgccgc caggccgcac gacccggcac atctgctcga
     1681 gacccccgtc cgcgtcggcg aggaagttca tcaccaactg agacacgacc acgtcgtatg
     1741 tcgcatccgg ccagggcagc ccctccgcga cgccctgacg tacgtcggcg ccgggcaccc
     1801 ggtccgcaca ggccgtgacg aaggactccg acgggtccgc ggcggcgaca tacgccgcac
     1861 cgagacggcg cgcgagcgcc tcggtcagcg cacccggccc gcagcccacg tcgagcgccc
     1921 gcatccccgg cgtgacaccg gcgaaggaga ggaaagccga ggcgagctgg tcggagtagc
     1981 ggcccatgta gcggtcgtag gcagcacggg acatggcgaa gctcatccgg gcattacccc
     2041 acgaggctcc ccctcggtca aggcgatggg ttgcgggtcg gtgacgccac gcccacggac
     2101 tgggcgagct gcgcgcggct acgttgacgc ctcgacaccc ggctggctgt tccctcccgc
     2161 ggcgacgcgg tgagcggctg atccggccta ctccgcggtc gatagcacgt ggcggcggaa
     2221 gagctcggcg gccggcagga gccggcgttc ggtcgaccac accaggccga cgtcgcgcac
     2281 cgcctcaggg tcggtgatgg gcacgaggcg caccgccccg ggtccggcga cggccgggtc
     2341 gtgccccatg gccggaacga tcgccacgcc caggccggcc accacgaacg cccgcagcgt
     2401 gggcaggtca tcggcctcga cggcgatctg cggctcgaag ccggccgccg cgcacagaac
     2461 gtgcgactgc tgacgcaaca gcgaggcgag gcgcagcgcc acgaagcgct cggccgacac
     2521 ctgcgccagg gtgatctcgg agcgcccggc cagcggatgc ccggccggca ccccgatgaa
     2581 cagcggctcg gagacgagcc gccgccagcg gacctgcggg cccaccgggc gacgggccgt
     2641 gatctcgagg tcgacccgcc cgccggcgag caccggcgag agcagctcgt cgcgcacctg
     2701 gctcaggacg aagtgcactc ccgggtgctc ggcacggaag ctgctcacca gctcgggcac
     2761 cagccatagc ccgagcgaga gctggaaggc gacggacacg atgccccgct cggggtcgac
     2821 gagctggtcg accgcggcca gaccgtcgtc gaggttgttg atgagggcat cgacatgccg
     2881 cttgaacgcc gacccggcgt gggtgagccg cagcacccgg ccggaccggc ccagcagcgg
     2941 cgtcccgacc tcgtcctcga gccgggcgag cgcccgcgag acgcccggct ggctcaccag
     3001 gtggatgtcg gccacctcgg tcaccgtcac cccgtcggcg acctgctgga accagcgcaa
     3061 tgcctcggtg tccacactca tgacgatact gcatgagtca tatatcagga cttcattgga
     3121 cgcatgggac ggactcattc acacttgaag catgaaccag atacagcaac gcatccaccg
     3181 cgcggtgtcg accagcggga tcgccccgcg ccttgccaag gtgcgcgagg agctggccgc
     3241 acgtcaggcc cagcgggagg cgcgccgtgc tctcgagcgt gagatcgccg gctacacctc
     3301 cgtgtccgac ctggctgacc tgcaggccgc gctcggccgc tccggcaccg aggccgacga
     3361 agtccgcgag atcctgcagc gacaccagtc cacgctggac caccgccggc tgcgcgcctg
     3421 ctgaccgtcg ccgtcctcgg ccgaccatgg cgccggggtg ccggcgtggg ccgcgccgtc
     3481 cacgacacga gccgtccacg actcgctcag tccacgac
//
LOCUS       DATAIX010000465         1649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_85300, whole genome shotgun sequence.
ACCESSION   DATAIX010000465 DATAIX010000000
VERSION     DATAIX010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85300"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VHM65_06990"
     CDS             complement(<1..346)
                     /locus_tag="VHM65_06990"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX2705487.1"
                     /translation="MPSTVMGVIARAKGAPVELVEIVVPDPGPGEAVVTVEACGVCHT
                     DLHYREGGINDAFPFLLGHEAAGTVEAVGEGVGDLAPGDFVILNWRAVCGQCRACRRG
                     KPWYCFATHNATQ"
     gene            complement(452..1492)
                     /locus_tag="VHM65_06995"
     CDS             complement(452..1492)
                     /locus_tag="VHM65_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX2705488.1"
                     /translation="MRAEDSFDLDAVAGWLRSARPDLPALSGTPELRQFGGGASNLTY
                     LLGYPGAAFVLRRPPAGTKAKSAHDMRREYLIQSRLRPRFPYVPTMVALCEDESVIGS
                     EFYVMEHLEGTILRRDLPPELGLTPSATTALCRAAVDLLVELHRVDVEAVGLGGLSRG
                     TGYVARQVAGWSGRYRAARTPDVASFEEVMGWLHENQPSDIRTCLIHNDFRFDNLILA
                     PGEPTRVIGILDWEMATVGDPLMDLGGALAYWAQAGDDPAFLALRRQPTHAPGMLTRA
                     EVLEHYLDRAGLRLTPEGWTFYEVFGLFRLAVIAQQIYFRFFHGQTSNPAYGAFLPAV
                     RYLDTRCTRLIG"
BASE COUNT          242 a          538 c          639 g          230 t
ORIGIN      
        1 tctgggtggc gttgtgggtg gcgaagcagt accagggctt gcccctcctg cacgcgcggc
       61 actgcccaca gaccgccctc cagttgagga tgacgaagtc acccggggcg aggtcgccga
      121 ccccctcgcc gacggcctcg accgtgccgg cggcctcgtg cccgagcagg aacgggaagg
      181 cgtcgttgat cccgccctcg cggtagtgca ggtcggtgtg gcagacgccg caggcctcga
      241 ccgtgacgac cgcctcaccc ggccccggat ccgggacgac gatctcgacg agctcgacgg
      301 gcgctccctt ggcgcgggca atgactccca tgacggtact gggcatgacc ggtccttcct
      361 gcgcggtggg tgggcgcagc gcggaggttc cggcggcgct gcggcgctgg gccgagacta
      421 gccagggggc cgaacctggc accaggggtg ctcagccgat gagccgggtg cagcgcgtgt
      481 cgaggtagcg gacggccggg aggaaggcgc cgtaggccgg gttgctcgtc tggccgtgga
      541 agaaccggaa gtagatctgc tgggcgatca cggcgagccg gaacaggccg aagacctcgt
      601 agaaggtcca cccctccggc gtgaggcgca ggccggcgcg atcgaggtag tgctcaagga
      661 cctcggccct ggtgagcatc ccgggagcgt gcgtcggctg gcggcgcagc gcgaggaagg
      721 cagggtcgtc cccggcctgg gcccagtagg ccagtgcccc gccaaggtcc atgagcgggt
      781 cgccgaccgt ggccatctcc cagtcgagga tgccgatgac gcgggtgggc tcgccggggg
      841 cgaggatgag gttgtcgaag cggaagtcgt tgtggatcag acaggtccgt atgtcgctcg
      901 gctggttctc gtggagccag cccatgacct cctcgaagct ggcgacgtcc ggtgtgcgcg
      961 cagcccggta gcgcccggac catcccgcga cctggcgcgc gacataccct gtgcctctgc
     1021 tcagcccgcc cagtccgacg gcctcgacat cgacgcggtg gagctcgacg agcaggtcga
     1081 cggcagcccg gcacagggcg gtggtcgctg acggggtgag cccgagctca ggtgggaggt
     1141 cccggcgcag gatcgtgccc tcgaggtgtt ccatgacgta gaactcgctg ccgatgaccg
     1201 actcgtcctc gcacagcgcg accatcgtcg gcacgtacgg gaacctggga cgcagccgcg
     1261 actggatgag gtactcccgg cgcatgtcgt gggcgctctt ggccttggtc ccggccggcg
     1321 ggcgacgaag gacgaaggcc gcgcccggat agccgagcag gtaggtgagg ttggacgccc
     1381 cgccgccgaa ctggcgcagc tcgggtgtgc cggacaaggc gggcaggtcc gggcgggcgg
     1441 accgtagcca gccggcgacc gcgtcgaggt cgaacgagtc ctcggcgcgc acgtcctgag
     1501 cgtcgtgggg ggtcaccggt gtcgcggcga cgccgggtcg cggtacttct tgagctcgag
     1561 ccgggcgatg acctctcggt gcacctcgtc cggcccgtca gccaggcgga gcgagcgggc
     1621 gctggccagg gccgcggcga gcgggaagt
//
LOCUS       DATAIX010000466         2619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_85427, whole genome shotgun sequence.
ACCESSION   DATAIX010000466 DATAIX010000000
VERSION     DATAIX010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85427"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1498)
                     /locus_tag="VHM65_07000"
     CDS             complement(<1..1498)
                     /locus_tag="VHM65_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2705489.1"
                     /translation="MDMETTAWMSMYHAMNAQDDRRPFRRATIARIARFARPHRARLA
                     AFLLLSIVVAVLAVATPVLAGRVVDAIVEAQPFSDVLRLATLIAVIAVAEAGLGLTAW
                     WLSSTIGEGLILDLRMTVFDHVQRMPIAFFTRTRTGALVSRLNNDVIGAQRAFSDTLS
                     SVLSNLVTLVLTLVVMLGISWQITLLALVLLPLFVVPARRMGSRLARYEREQANHNAA
                     MSTQMTERFSAPGATLVKLFGHPGRESGEFAARARRVRDIGVRITLAQGVFYTALTLV
                     AALALALIYGLGGYYALNGQLDAGAVVSLALLLTRLYAPLTALASARVEVMSALVSFE
                     RVFEVLDLVPLIQDRPGAVTVPDGPVSVELDHVRFSYPAADKVSLASLEEVAKLDNRG
                     GVEVIHDISFRAEPGQLVALVGSSGAGKSTIAQLLPRLYDVDAGAVRLAGVDVRDITA
                     ASLRHTIGLVTQDGHLFHESVRSNLLLANPEADEVELWDALRRAGPRPR"
     gene            complement(1802..2209)
                     /locus_tag="VHM65_07005"
     CDS             complement(1802..2209)
                     /locus_tag="VHM65_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX2705490.1"
                     /translation="MTGRPSRGLLDTSVLIASETGRALDLSALPDEMFVSVVTVAELQ
                     AGVLAAPDTATRARRLATLESIAVLEPLPIDSAAAAQWAVLRARLAEEGRRINVNDLW
                     IAAVAAANNLAIVTQDSDYDPLAAIGGPRVIRV"
     gene            complement(2230..2490)
                     /locus_tag="VHM65_07010"
     CDS             complement(2230..2490)
                     /locus_tag="VHM65_07010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX2705491.1"
                     /translation="MSVVASRDLRNHTAEVLRRVAQGEQVTVSVNGTPVAEISPVRAR
                     RRMSLSRGELTAVLERSQADPGLRADLQRLAGESTDDLGPIR"
BASE COUNT          412 a          901 c          959 g          347 t
ORIGIN      
        1 gacgtggtcg agggcctgcc cggcgcagcg cgtcccagag ctcgacctcg tccgcctcgg
       61 gattggccag cagcaggttg gaccgcaccg actcgtggaa gaggtgcccg tcctgggtga
      121 ccaggccgat ggtatggcgc agcgacgcag ccgtgatgtc gcggacgtcc accccggcca
      181 ggcgcacggc acccgcgtcg acgtcataca gcctgggcag gagctgggcg atcgtggact
      241 tgcccgcgcc ggacgagccg accagggcga cgagctgacc cggctcggcg cggaaggaga
      301 tgtcgtggat gacctcgacc ccgccgcggt tgtcgagctt ggcgacctcc tcgagcgagg
      361 cgagggagac cttgtcggcg gcggggtagg agaagcggac atggtcgagc tcgacgctga
      421 cggggccgtc gggcacggtg acggcgcccg gcctgtcctg gatgagcggc accaggtcca
      481 ggacctcgaa gacgcgctcg aagctcacca gcgcgctcat cacctcgacg cgggcgctgg
      541 ccagggccgt gagcggagcg tagagccggg tcagcagcag cgccagggag acgacggccc
      601 cggcgtcgag ctggccgttc agggcgtagt agccgcccag cccgtagatc agcgcgagcg
      661 ccagcgcggc cacgagggtc agcgcggtgt agaagacgcc ctgcgccagg gtgatacgga
      721 ccccgatgtc acgcacccga cgggcccgcg cggcgaactc gccggactcc cggccggggt
      781 gcccgaagag cttgaccagg gtcgcgcccg gcgccgagaa gcgctccgtc atctgggtgc
      841 tcatcgccgc gttgtggttg gcctgctcac gctcgtaccg cgccagccgg gaccccatgc
      901 gccgggccgg gaccacgaac agcggcagca ggacgagggc cagcagcgtg atctgccagg
      961 agatgccgag catcaccacc agggtgagga cgagggtgac caggttggac agcacgctgg
     1021 acagcgtgtc gctgaacgcg cgctgcgccc cgatgacgtc attgttgagc cggctcacga
     1081 gggccccggt gcgggtgcgg gtgaagaacg cgatcggcat ccgctgcacg tggtcgaaga
     1141 ccgtcatgcg caggtccagg atcagcccct cgccgatggt cgaggacagc caccaggccg
     1201 tcagcccgag tccagcctcg gcgaccgcga tgacggcgat cagggtggcc agccgcaaga
     1261 cgtccgagaa gggctgggct tcgacgatgg cgtcgacgac acgccccgcg aggacgggcg
     1321 tcgccaccgc gaggaccgcc acgacgatgc tgagcagcag gaacgccgcg agccgggccc
     1381 ggtggggtcg ggcgaaccgc gcgatgcggg cgatcgtcgc cctgcgaaac ggcctgcggt
     1441 cgtcctgggc gttcatggcg tggtacatcg acatccacgc tgtcgtctcc atgtccatct
     1501 gcggcaccca tctgcaaggg agcggccggt cattggggcg ccgaccgccg ggctacgagc
     1561 ggggtcaagt cgaggacgtc aacgcgggtt caggcctgga catgccccca gcacatgccc
     1621 gcgggccgtg atggggcgag gagggcctct caatgggccg tctagcggcg gtcacagccc
     1681 ttgcgggtgg acaacgagtt cgccctcctc gccaagtcga gcattcctcg gggcccgagt
     1741 cgtgtcaagg ggtggttcac cagcgcggcc cccgcgccag ccgaaccgtc agactgggca
     1801 gtcagacgcg gatcacgcgt gggccaccga tggccgcgag cgggtcgtag tcgctgtcct
     1861 gggtcacgat cgcgaggttg ttggcggcgg cgacggcggc gatccacagg tcgttgacgt
     1921 tgatccgccg tccttcctca gccagccggg cccgcaggac cgcccactgc gcggcggctg
     1981 cgctatcgat cggcaagggc tccaggacgg caatggactc caacgtcgcg agcctgcggg
     2041 cccgtgtcgc tgtatccggc gccgccagca cccccgcctg cagctccgcc acggtcacga
     2101 cgctgacgaa catctcgtcg ggcagggcgc tgagatccag ggcgcggcct gtctcgctcg
     2161 cgatgaggac gctggtgtcg agcagccccc gcgagggccg gcccgtcacc gggcctgtca
     2221 ccgggtccgt caccggatgg ggcccagatc gtcggtgctc tccccggcca gccgctgcag
     2281 gtccgcgcgc agccccggat cggcctggga gcgctccagg acggcagtga gctcgccgcg
     2341 cgacagcgac atgcgccggc gtgcccgcac cggggagatc tcagcgaccg gagtcccgtt
     2401 gacgctgact gtgacctgct cgccctgagc aacccgacgg agcacttccg cggtgtggtt
     2461 gcgcaggtcc cgcgatgcga cgacagacat gtgtgcaaat gtagcacttc cgccacaccc
     2521 tcgggtatgg cggcccgctc accgccgacg caacaccggc gggccaccca cggcggtgcg
     2581 cggaccggca taccggctgg gttggctagc ctttcggag
//
LOCUS       DATAIX010000467         4743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_249416, whole genome shotgun sequence.
ACCESSION   DATAIX010000467 DATAIX010000000
VERSION     DATAIX010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249416"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2335)
                     /locus_tag="VHM65_07015"
     CDS             complement(<1..2335)
                     /locus_tag="VHM65_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705492.1"
                     /translation="MQSIPVFDTIEGLTVLRDDDDFAHFFYLPRTLRVARSGDGKPMF
                     SFLKYQFPVERETEDAKGGGYLVFTTQLVEDMEFLETKVKPKLTDRMRAERPNDPQLP
                     PPKLTAIDFVTGEVRLLIMKDDKFVGTVQAGKPSMFADNSASFAVELKDLGAELLYQA
                     LRKGGGVAVVEYDLGFETRLPAVHVYAHCDSNEVKDVVMTYTTSQITSEDTWGNEETT
                     SEAHRTGISEVMESQGLVTLNIDKGSSQIKDEDVEALRGFAFSKLDGWLKDHFLKGGS
                     FATAADRESQWMSFIHEDTHQVFNLDLVQRDVVIRQYNPSATLTPAFLGADVDDVVLD
                     IDLGTAEWYFNTLSVTVDTNLSFDTYGDIVHSVVGHFSYEGTKDGHNITKRESFAFTK
                     ADRAPKVFTTRLAAVGQDTYSVDVEVNYISGPVTTTRLYSDKDHVRNYTLRVPNPGVM
                     NLRVAASDTTAFDTQKLSSIEVEIRYADAARHVPEVVDTVILTKAVPEAVYRRVIYAP
                     WDQPYHYRVTYVTTDEVSGSQRITGDWLLGEHDDNAQSGYLSISTPFDDGFHLSVVPS
                     VDWSEVQNLIVDLDYLDEAHDYRQSRTLNFSESTLSALPPPQWRFLLRDPDARSYRYS
                     TKTVRKDGSVVSDEWAPVETDSSTLVVGNAVGGVVKVSVDPSDSGAGTTMRRVIVRLS
                     YVDDAHGIHDTQALVFSDTTAQVWSVARADASVTAYAYDVQYVPLTGEPVSVTGLSGT
                     LSAVNDFLFVPAPPAPPAPPAPVPPAPVPPARTPPAPA"
     gene            2481..3191
                     /locus_tag="VHM65_07020"
     CDS             2481..3191
                     /locus_tag="VHM65_07020"
                     /inference="COORDINATES: protein motif:HMM:NF021021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HEX2705493.1"
                     /translation="MVSLGEAGQRKGRFTLVVLADGFPDEGLPRYVDVVRAVRRELLG
                     TAPFSTLRALLSVSRIDRTGPSDLGVTFGLTDERLVRLDEERARRIASEYGAAPDVVL
                     VVVDSPRYAGYGGAGIAAVTVHPDAPRLAVHEIAHAGFDLADEYGGDGPAASGNGEPH
                     RVNVSRVGDPSVVKWRDLVTGDGTVGCFEGGDRTSYGMFRPSPRCLMRSVHDEFCPVC
                     RREIARQLVFFAQGRSPT"
     gene            3272..4456
                     /locus_tag="VHM65_07025"
     CDS             3272..4456
                     /locus_tag="VHM65_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemopexin repeat-containing protein"
                     /protein_id="HEX2705494.1"
                     /translation="MTAKRVYFFSGSDYVRFDHPLDNVLPIYPLSTATMWPGLTPPID
                     AGLNWGNGKVYFFSGASYWRYDTRLDAVDPLYPRPVGLAWTDAASAHFGAGIDAAVNW
                     GNGKAYLFKGGEYIRHDIAADTLDPGYPKPIAGNWGGVAGTIFERGIDAIVNWGNGKV
                     YWFKDDCYGRFDQGTKALDPGYPKLIAGNWPGVFAAGLAGALEWPMAAVAAGGFRVPE
                     HQSPCNVIPAVGGTNFNQYFEMNIDFDPAAAYPATCAVGEYRQYVRGQFRKNGVVKVH
                     LLADFRGGPAPAMLPAPPVGSPFDNFIEDGVIFPGPPPVNSLYGHRIDELGNADATNQ
                     FTPDRLTGCTYRGWDRPGWGGRTGETIAINLDFRGQAVDAASGGEVLNTADWSVNCGG
                     VA"
     gene            4456..>4743
                     /locus_tag="VHM65_07030"
     CDS             4456..>4743
                     /locus_tag="VHM65_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705495.1"
                     /translation="MTESQLIAGRWVLPTVESNRDGVVRMHVSVSVAEPDALADVPID
                     VAVSGAGQQLAPRETPTPADYYYLQTRAVTAVADFAFDNPGGADPDTVTVSM"
BASE COUNT          708 a         1560 c         1675 g          800 t
ORIGIN      
        1 gtgcgggtgc gggtggcgtc cgcgcgggtg gcaccggtgc gggtggcacc ggcgcgggtg
       61 gtgccggcgg tgcgggtggc gccgggacga agaggaagtc gttcaccgcg ctcagcgtgc
      121 ccgacaaccc ggtgaccgac accggctcgc cggtgagcgg cacatactgc acgtcatacg
      181 cgtatgccgt gacgcttgcg tcggcgcggg cgaccgacca gacctgggcc gtggtgtcgg
      241 agaagacgag ggcctgggtg tcgtgaatgc cgtgggcgtc gtcgacatag ctgagccgca
      301 cgatgacgcg gcgcatcgtg gtcccggccc cgctgtcact cgggtcgacc gagaccttga
      361 cgacaccgcc caccgcgttg ccgacgacga gcgtcgagga gtcggtctcg accggtgccc
      421 actcgtcgga gaccaccgag ccgtccttgc ggaccgtctt ggtgctgtac cggtaggacc
      481 gggcgtcggg gtcacggagc aggaagcgcc attgcggcgg cgggagcgcg ctgagcgtcg
      541 actcggagaa gttcagggtg cgggactgcc ggtagtcgtg ggcctcgtcg agatagtcga
      601 ggtcgacgat gaggttctgg acctcggacc agtccaccga agggaccacc gagaggtgga
      661 agccgtcgtc gaacggcgtc gagatcgaga ggtaccccga ctgggcgttg tcgtcgtgct
      721 ccccgagcag ccagtcgccg gtgatgcgct ggctcccgga gacctcgtcg gtcgtgacgt
      781 acgtgacccg gtagtggtag ggctggtccc agggcgcgta gatgacccgc cggtagacgg
      841 cttcggggac cgctttggtg aggatcaccg tgtcgacgac ctccggaacg tgcctggcgg
      901 cgtcggcgta cctgatctcg acctcgatcg agctgagctt ctgggtgtcg aacgcggtcg
      961 tgtcactggc agcgacccgc aggttcatca cgccggggtt gggcacccgc agcgtgtagt
     1021 tgcggacgtg gtccttgtcc gagtagagcc gcgtcgtggt gaccgggccg gagatgtagt
     1081 tgacctcgac gtcgaccgag taggtgtcct ggccgaccgc ggccagccgc gtcgtgaaga
     1141 ccttgggagc ccggtcggcc ttggtgaacg cgaagctctc gcgcttggtg atgttgtgcc
     1201 cgtccttggt gccctcgtag ctgaagtggc cgacgacgct gtggacgatg tcgccatacg
     1261 tgtcgaagct gaggttcgtg tcgaccgtga cgctgagggt gttgaagtac cactcggccg
     1321 tgccgaggtc gatgtcgagg acgacgtcgt cgacatcggc gccgaggaag gccggggtga
     1381 gggtggcact ggggttgtac tggcggatca cgacgtcacg ctggacgagg tcgaggttga
     1441 agacctggtg ggtgtcctcg tggatgaagc tcatccactg gctctcccgg tcggccgcgg
     1501 tcgcgaagct gccgcccttg aggaagtggt ccttgagcca gccatcgagc ttggagaacg
     1561 cgaacccccg cagtgcttcg acgtcctcgt ccttgatctg gctcgaaccc ttgtcgatgt
     1621 tgagggtgac caggccctgg gactccatga cctcgctgat gccggttcgg tgggcctcgc
     1681 tcgtcgtctc ctcgttgccc caggtgtcct cgctcgtgat ctggctcgtc gtgtaggtca
     1741 tgacgacgtc cttgacctca ttggagtcgc agtgcgcgta gacgtgtacc gccggcagcc
     1801 gggtctcgaa gccgaggtcg tactcgacga cggcgacccc gccgcccttc ctgagggcct
     1861 ggtagagcag ctcggcgccg aggtccttga gctcgacggc gaacgacgcg ctgttgtcgg
     1921 cgaacatgct cggcttgccg gcctggacgg tcccgacgaa cttgtcgtcc ttcatgatga
     1981 gcaggcggac ctcgccggtg acgaagtcga tcgcggtgag cttgggtggc ggcagctggg
     2041 ggtcgttggg ccgttcggcc cgcatccggt cggtgagctt gggtttgacc ttggtctcga
     2101 ggaactccat gtcctcgacg agctgcgtgg tgaagacgag gtatccgccc cccttggcgt
     2161 cctcggtctc gcgctcgacc gggaactggt acttcaagaa cgagaacatc ggtttgccgt
     2221 cgccgctgcg ggccacgcgc agcgtccggg ggaggtagaa gaagtgagcg aagtcgtcgt
     2281 cgtccctcag gacggtcagg ccctcgatcg tgtcgaagac cgggatggac tgcatcgggc
     2341 tgcctccatg cgacaccgtg cgccgcagag caaagcgccc gggcggggac tggcgatgtt
     2401 agggacggta cgctgacgaa gcggcggacg ccatagagag gacggtccgg aaccccctga
     2461 tacatcgtgg aatcggtgct ctggtcagcc tcggcgaggc cggccaacgg aaagggcgtt
     2521 tcacccttgt ggttctcgct gacggcttcc ccgacgaggg gctgccgcgg tatgtcgacg
     2581 tcgtgcgagc cgtgcgccgc gagctccttg gcacggcgcc attctcgacg ctgcgcgccc
     2641 tgctcagcgt gtcgcggatc gaccggaccg gcccctccga cctcggggtg accttcggcc
     2701 tcaccgacga gcggctggtc cgcctcgacg aggagcgagc gcgcaggatc gcgagcgagt
     2761 atggcgcggc gccggatgtc gtcctcgtcg tcgtcgactc gccccggtac gccgggtacg
     2821 gcggcgcggg catcgccgcc gtcacggttc atccggacgc cccccggctc gcggtccacg
     2881 agatcgccca tgccggcttc gacctggccg acgagtacgg cggtgacggt cccgccgcct
     2941 cggggaacgg cgagccgcac cgggtcaacg tctcccgggt gggtgacccc tccgtcgtga
     3001 agtggcgcga tctggtgacc ggcgacggca ccgtggggtg cttcgagggg ggcgaccgga
     3061 cgtcatacgg gatgttccga ccgagcccac ggtgcctcat gcgctcggtc cacgacgagt
     3121 tctgtcccgt atgccggcgc gaaatcgccc ggcagttggt attttttgcg cagggccggt
     3181 ctccgacgta gtggacggcg ccccggcgtg acatgggacc gacccggcta gctcatcgcc
     3241 agacccgact cgacagtccc taggagacgc catgacggcc aagcgggtgt acttcttctc
     3301 aggttctgac tatgtccgct tcgaccatcc cctggacaac gtcctgccga tctacccctt
     3361 gagcacggcc acgatgtggc ccggtctcac ccctccgatc gacgcgggcc tgaactgggg
     3421 caacggcaag gtctacttct tctcgggcgc gtcctactgg cgctacgaca cccgcctcga
     3481 cgccgtcgac ccgctctacc cgcgcccggt cgggctcgcc tggacggacg ccgcctcggc
     3541 gcacttcggc gcggggatcg acgccgccgt caactggggc aacggcaagg cctacctctt
     3601 caagggcggc gagtacatcc ggcacgacat cgcggccgac acgctcgacc cgggctaccc
     3661 caagccgatc gccgggaact ggggtggcgt ggccgggacc atcttcgaac ggggcatcga
     3721 cgcgatcgtc aactggggca acggcaaggt ctactggttc aaggacgact gctacggccg
     3781 gttcgaccag ggcaccaagg cactcgaccc gggctacccg aagctgatcg ccggcaactg
     3841 gccgggcgtc ttcgccgccg gcctcgccgg tgccctcgaa tggcccatgg ccgcggtggc
     3901 cgcgggaggg ttccgggtgc ccgagcacca gtccccgtgc aacgtcatcc ccgcggtcgg
     3961 cgggacgaac ttcaaccagt acttcgagat gaacatcgac ttcgacccgg ccgcggcgta
     4021 tcccgcgacc tgcgcggtgg gcgagtaccg ccagtacgtc cgggggcagt tccgcaagaa
     4081 cggcgtggtc aaggtgcatc tgctcgccga cttccgcggc ggcccggctc ccgcgatgct
     4141 gccggcgccc ccagtgggct cgccgttcga caacttcatc gaggacggtg tcatcttccc
     4201 cggacccccg ccggtcaaca gcctctacgg gcatcgcatc gacgagctcg gcaacgccga
     4261 cgcgaccaac cagttcaccc cggatcggct caccggctgc acgtaccgcg ggtgggaccg
     4321 gcccggctgg ggcgggcgga ccggggagac gatcgccatc aacctcgact tccgtggcca
     4381 ggcggtcgac gccgcctcgg gtggagaagt gctcaatacc gccgactggt cggtcaactg
     4441 tggaggggtg gcgtgatgac ggagtcccag ctcattgccg ggcggtgggt cctgccgacg
     4501 gtcgagagca accgtgacgg agtcgtccgg atgcacgtca gcgtctcggt cgccgagccc
     4561 gacgcgctcg ccgacgtgcc gatcgacgtg gccgtctccg gcgcaggaca gcagctcgcc
     4621 cctcgcgaga cgccgacacc ggccgactac tactacctgc agacccgcgc cgtcaccgcc
     4681 gttgccgact tcgccttcga caacccgggc ggcgccgacc ccgacacggt caccgtgtcg
     4741 atg
//
LOCUS       DATAIX010000468         1973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_174671, whole genome shotgun sequence.
ACCESSION   DATAIX010000468 DATAIX010000000
VERSION     DATAIX010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174671"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VHM65_07035"
     CDS             complement(<1..844)
                     /locus_tag="VHM65_07035"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX2705496.1"
                     /translation="MPALTAVSTPAGAAQTWELWSCTMRLVVGEPAALPAARRLVDRE
                     LQAVEDAASRFRPDSEITALAARAGRPTRISQTLANLLGAALDAARDTGGAVDPTLGH
                     PLAALGYDRDIALVRAAGPGVTVRRRPSPGWTRIHLDTERREVTVQDGVVIDVGATAK
                     AWAADRCARVVAHTLGTPVLVSLGGDIATAGGDPGRPWQILVQDRREDPAAQIGLAVG
                     AAVATSSTQSRQWHCEGHQLHHILDPRTALPVAPVWRSVSVVADSALEANTWSTAAIV
                     RGEQA"
     gene            complement(844..1086)
                     /locus_tag="VHM65_07040"
     CDS             complement(844..1086)
                     /locus_tag="VHM65_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705497.1"
                     /translation="MSSARERGRRRISQGTAVLALVTVGAAGAGTALAALAVSDGSTT
                     ATATTQTTGTAAAATGTTSGQVSAGTGQAQAQASGS"
     gene            1457..>1973
                     /locus_tag="VHM65_07045"
     CDS             1457..>1973
                     /locus_tag="VHM65_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX2705498.1"
                     /translation="MTQSPATTPGRRPTPGRLMAAEIAEQPQVWRRLLAESPDALDAA
                     AAAVRAYRPRFVLLVARGTSDHAALYARYLIETRLELPAGLVSPSTMTAYGARPDLRE
                     VLMIGVSQSGGSPDLVRSAEVAREQGALTLSVTNNPSSALADAAQCHLDIGAGAELAV
                     AATKSYTSELLT"
BASE COUNT          245 a          731 c          739 g          258 t
ORIGIN      
        1 ccgcctgctc gcctctgacg atggcggccg tcgaccaggt gttggcctcg agcgccgagt
       61 cggcgacaac gctcaccgag cgccagaccg gtgcgaccgg cagggcggtc cgcgggtcga
      121 ggatgtggtg gagctggtgg ccctcgcaat gccattggcg cgactgcgtg cttgacgtgg
      181 caacggccgc gccgacagcc agaccgatct gcgccgctgg gtcctcccgc cgatcctgga
      241 ccaggatctg ccagggccgc ccagggtcac cgccggccgt ggcgatgtcg ccaccgaggc
      301 tgaccaggac gggcgtgccg agcgtatggg ccaccacccg ggcgcagcgg tcggcggccc
      361 acgccttggc ggtcgcgccg acgtcgatga cgaccccgtc ctgcacggtg acctcgcgac
      421 gctcggtgtc caggtggatg cgcgtccagc caggggacgg gcggcggcgc acggtgacgc
      481 cagggccagc cgctcggacg agggcgatgt cccggtcata gccgagggcg gcaagggggt
      541 gcccgagcgt cgggtcgacg gcgccgccgg tgtcccgcgc ggcgtcgagc gcggcgccga
      601 gcaggttggc cagcgtctgg ctgatccggg tcgggcgccc ggctcgcgcg gcgagcgccg
      661 tgatctccga gtcggggcgg aaccggctcg cggcgtcctc cacggcctgc agctcccggt
      721 cgacgaggcg gcgggcggcg ggcagcgcgg ccggctcgcc gacgaccagg cgcatcgtgc
      781 acgaccacag ctcccaggtc tgcgccgcgc cggccggcgt cgacacggcg gtgagggccg
      841 gcatcacgaa ccgctggcct gggcctgggc ctggccggtg ccggccgaga cctggccgga
      901 ggtcgtgccg gttgcggcgg ccgcggtccc tgtggtctgg gtcgtcgcgg tcgctgtcgt
      961 cgagccgtcg ctgacggcga gggcggccag agccgtgccc gcgccggccg cgcccacggt
     1021 gacgagtgcc agcacggcgg tgccctgcga gatcctgcga cggccacgtt ctcgtgcgct
     1081 gctcatgatg gtcggtcctc ctggtcgatg gaggttccac cgtcgcgcgc cagcctgtgc
     1141 cgctgctgtc cgttgtccat ggtccgggta gggaagtcgc ccaggcacgt ctcgtgcgcg
     1201 tcgcgcacgg gcgacgggcc aggcggtgac gagggtgcgg cgagagcctc gccgggtgct
     1261 gtcccaggct gccgggtgca cccgtgatcg ggctggacca cactggcact ctggaccaga
     1321 ccggcaccac tggaccagac cggcaccact ggaccgacac cagttgttgc ctggaggtcc
     1381 gggcctcccg ctcgggtccc ggcccccatc acgattcgtc gaagcgccac ggccaccgag
     1441 cacaggacgg acgcccatga cccagtcacc cgccaccacg cccggccgca ggcccacccc
     1501 gggacggctc atggccgccg agatcgccga gcagccccag gtatggcgac gcctcctggc
     1561 cgagagcccg gacgcgctcg acgcggcggc cgccgcggtg cgcgcgtacc ggccccggtt
     1621 cgtgctcctc gtcgcccgag gcacctccga tcacgccgcg ctctacgcga ggtacctcat
     1681 cgagacccgg ctcgagctcc cggccgggct cgtctcgccc tcgacgatga ccgcgtatgg
     1741 cgcccggccg gacctgcgtg aggtgctcat gatcggcgtg agccagtccg ggggctctcc
     1801 ggacctcgtc cgcagcgccg aggtggcccg cgagcagggc gccctcaccc tgtcggtgac
     1861 gaacaacccg tcgtcggcgc tggctgacgc cgcccagtgc catctcgaca tcggggcagg
     1921 ggccgagctg gcggtggccg cgacgaagtc ctacacgagc gagctgctca cgc
//
LOCUS       DATAIX010000469         1758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_249455, whole genome shotgun sequence.
ACCESSION   DATAIX010000469 DATAIX010000000
VERSION     DATAIX010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249455"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VHM65_07050"
     CDS             complement(<1..224)
                     /locus_tag="VHM65_07050"
                     /inference="COORDINATES: protein motif:HMM:NF015119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HEX2705499.1"
                     /translation="MRRRIFGIENEYGVTCTFEGQRRLTPDEVARYLFRRVVSWGRSS
                     NVFLPNGSRLYLDVGSHPEYATAECDSVRQV"
     gene            299..>1758
                     /locus_tag="VHM65_07055"
     CDS             299..>1758
                     /locus_tag="VHM65_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705500.1"
                     /translation="MSLAQGAPEPRVDEAHAQIGDVSRHALSILSRAQTYADQLRSQV
                     EDEVAATRSGAGELMAQARALHHAADSLHADARAAHDQAAARLAEAHEDAEQLLADSS
                     DQAALVTESAGRTSEEAVSAARAEADQILAAAQAHAAQVRGSAQGDYDRAMAVVAAHE
                     AERTAQHDDRFAALAAEEARRLGEADASSEGIRSQAEAYAESVRFAAQAEADTWQAEA
                     GRVVDEARAEADRLVSEAKVTAQDVTERAQRESDDLMAAAMSHMAWAQTTMRQLLDQA
                     EEEVARLKTGTYRDLARHTRRRRQALQAVVARVTYRSREHLDTATSEAARQRGQAQAI
                     LEAAQADADHTAVAAQAQADRVVREAEVTAAAHVERGDRRLREAEHGAKVVRDRAAAE
                     VERLQREAHENRRQAREELIRTISEARAEADGLRSQAHEMLDRARSEVTALAARRNDI
                     TTQLGHLSGVIEALAVPDRPEDAAPEHVGHDAPVPST"
BASE COUNT          281 a          643 c          649 g          185 t
ORIGIN      
        1 acctgacgca ccgagtcgca ctcggcggtg gcgtactccg ggtgcgaccc gacatcgaga
       61 tagaggcggg acccgttggg caggaacacg ttgctcgagc ggccccacga gacgacgcgg
      121 cggaacaggt agcgggccac ctcgtccggg gtgaggcgcc gctggccctc gaaggtgcag
      181 gtcacgccgt actcgttctc gatcccgaag atacgccggc gcatgcctgc actgtatgac
      241 gtgatgtcgc cgcatgacgt gatgtcgccg cgccgggggt tctggaatca tgggcgtcat
      301 gtcgttggcc caaggcgccc ccgaaccccg ggtggacgag gcgcacgcgc agatcgggga
      361 cgtcagccgc cacgcgctgt cgatcctgtc gcgggctcag acgtatgccg accagctgcg
      421 ctcacaggtg gaagacgagg tcgcggcaac ccggtccggg gccggcgagc tcatggccca
      481 ggcccgggcg ctgcaccacg cggccgactc gctgcatgcc gatgcccgcg cagcccacga
      541 ccaggctgcc gctcggctcg cggaggccca tgaggacgcg gagcagctgc tggccgactc
      601 ctccgaccag gccgcgctcg tcaccgagag cgccgggcgc acgagcgagg aggccgtatc
      661 ggcggcccgg gcggaggccg accagatcct cgcggccgcc caggcccacg ccgcgcaggt
      721 ccgcggaagc gcccagggcg actacgacag ggccatggcc gtcgttgccg cccacgaggc
      781 cgagcgcaca gcacagcacg acgaccgctt cgcggccctg gcggccgagg aggcccgccg
      841 gctcggggag gccgacgcat ccagcgaagg gatccgcagc caagccgagg cgtatgccga
      901 gagcgtgcgc ttcgcggccc aggccgaggc tgacacgtgg caggccgagg cgggacgcgt
      961 cgtcgacgag gcccgggccg aagcggatcg tctcgtcagc gaggcgaagg tcaccgccca
     1021 ggacgtcacc gagcgcgccc agcgcgagtc cgacgacctc atggcggcgg ccatgtcgca
     1081 catggcctgg gcccagacca cgatgcgtca gctgctcgac caagccgagg aggaggtggc
     1141 ccggctcaag accggcacct atcgcgacct ggcccggcac acccggcggc gccgacaggc
     1201 actgcaggca gtcgtggccc gggtgaccta ccgcagtcgc gagcatctgg acacggcgac
     1261 ctcggaggcg gctcggcagc gcggtcaggc ccaggccatc ctcgaggcgg cccaggccga
     1321 cgccgaccac acggcggtgg ccgcccaggc ccaggccgac cgggtggtcc gcgaggcaga
     1381 ggtcaccgcc gcggcgcatg tcgagcgggg ggaccggcgc ctgcgcgagg ccgagcatgg
     1441 cgccaaggtc gtgcgtgacc gggccgcagc cgaggtcgag cggctgcagc gtgaggcaca
     1501 cgagaaccgc cgccaggccc gcgaggagct catccgcacg atcagcgagg cgcgggcgga
     1561 ggccgacggg ctgcgctcgc aggctcacga gatgctggac cgggcccgct cggaggtgac
     1621 tgctctcgcc gctcgccgca acgacatcac cacccagctc ggtcatctgt ccggggtcat
     1681 cgaggccctc gccgtgccgg accggcccga ggacgccgcg cccgagcacg tcggccacga
     1741 cgctcccgtc cccagcac
//
LOCUS       DATAIX010000470         3190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_25247, whole genome shotgun sequence.
ACCESSION   DATAIX010000470 DATAIX010000000
VERSION     DATAIX010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25247"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1195)
                     /locus_tag="VHM65_07060"
     CDS             complement(164..1195)
                     /locus_tag="VHM65_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX2705501.1"
                     /translation="MSTEPAARVSPLLSRPGAVAGAGADARVAAHYGDPMREQRLLTE
                     GLAVVDLSHRDVLTVTGADRLSWLHSLTTAHLTGLAPYESRESLVLSPNGHIEHALHL
                     VDDGTTTWVGVEPGTGPALWAWLDSMRFMLRVEVKDVSAAWAVLGEPIGSESVAGEPI
                     AWRDPWPGLVGDTAAYGPVEGHPATGRVWRELVVPRDRIEQAVGDRPLAGLWAAEALR
                     VAAWRPRMGADTDHRTIVHELDWLRTAVHLHKGCYRGQETVARVHNLGRPPRRLVFLH
                     LDGSAHTVPQAGAPVSVAGRVVGHLTSVARHYELGPIALALVKRNTDLTATLDAGGYA
                     ATQEAIVAP"
     gene            complement(1168..1596)
                     /locus_tag="VHM65_07065"
     CDS             complement(1168..1596)
                     /locus_tag="VHM65_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX2705502.1"
                     /translation="MAELGETLRAHGRRLTPQRRRIIGALAALEHATPDDVVGFVAQD
                     GGSPVPASTVYRTLEALEELGIVSHTHLDHRSPSYHLAEHANHIHLVCLGCAQVESVP
                     ASSASTFVGNLRDRLGFEADVTHMAIHGWCAACRPNRPPE"
     gene            complement(1604..2101)
                     /locus_tag="VHM65_07070"
     CDS             complement(1604..2101)
                     /locus_tag="VHM65_07070"
                     /inference="COORDINATES: protein motif:HMM:NF020348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HEX2705503.1"
                     /translation="MAFAIDPTLAAELVPVAWLVGRWEGAGVVGYPTIESRHFGQELV
                     VSHDGRGFLEWQSRAWLLDAGGTRVKSSAVELGFLRPAPDGEVELLLVHPTGILELYY
                     GPVSPARIELRTDGVMRSPQAKEYSAARRVYGLVDSALMWVMDMAAVGQPLQSHLSAQ
                     LKRVG"
     gene            2164..2565
                     /locus_tag="VHM65_07075"
     CDS             2164..2565
                     /locus_tag="VHM65_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX2705504.1"
                     /translation="MDESPAGTAGPRPGLRPLVVKVTCGVEAVERLSQAFTVAATAAL
                     AGAPVSVWLTGEAVWLAVPGRAEDVELAHSTPLAALRDDLLDAARITACTQCAARRGL
                     AAQDLLPGIQIRGSASFVEEVLGAQAQALVY"
     gene            complement(2588..>3190)
                     /locus_tag="VHM65_07080"
     CDS             complement(2588..>3190)
                     /locus_tag="VHM65_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705505.1"
                     /translation="VVPSPRSGTAVTVFGSLTCVVAAAATLDDPLLRWLPIALAISTT
                     ASFAHQLLRGDHRPRLTESVAGSVSAVAIVASGVAMIPLGSLRHGAAVVLAVMLAVAG
                     AVLVDLLARWESLRPFLVVTAMIVGALAGLGVALATTSLPELPLALVGAVAAATSAAA
                     RRVLVIPPAAASRAGAAAVAAAPVLVVGVLAYLVDLVFIT"
BASE COUNT          455 a         1150 c         1188 g          397 t
ORIGIN      
        1 cccgtggggg ccaccgtgat cagggtcgag gtggcggagc gcatggccct catcatgcca
       61 ggggcgcgca gagagcgacc cgctgccggc gaggtcgggc aatgtccgcc catgtgccct
      121 ggatgaccgg acattgcccg acctcgggca aatggggcgg ggctcacggg gcgacgatgg
      181 cctcctgggt ggcggcatac cctccggcgt cgagggtcgc cgtgaggtcg gtgttgcgct
      241 tgaccagggc cagggcgatc gggccgagct cgtagtggcg cgccaccgag gtgaggtgcc
      301 cgacgacgcg cccggcgaca ctgaccgggg cccccgcctg gggcaccgtg tgagccgagc
      361 cgtcgaggtg gaggaagacg agccggcgcg gtgggcgccc caggttgtga acccgcgcca
      421 cggtctcctg cccgcgatag cagcccttgt gcaggtgcac cgccgtccgc agccagtcga
      481 gctcgtggac gatcgtgcga tggtcggtgt cggcgcccat tcgcggccgc caggcggcga
      541 cgcgcagcgc ctcggcggcc cagaggccgg ccagcggacg gtcacccacc gcctgctcga
      601 tccggtctct gggcacgacc agctcacgcc agacccgccc ggtcgcgggg tgcccctcga
      661 ccggtccata ggccgcggtg tcaccgacca gccctggcca cgggtcacgc cacgcgatcg
      721 gctcccccgc cacggactcg gacccgatcg gctcaccgag cacggcccac gccgcgctga
      781 cgtccttgac ctcgacccgg agcatgaagc gcatcgagtc gagccaggcc cacagcgccg
      841 gccccgtgcc aggctcgacg ccgacccacg tggtcgtgcc gtcgtcgacg aggtgcagcg
      901 cgtgctcgat gtgcccgttg gggctcagga ccaaggactc gcgggactcg tatggcgcca
      961 gtccggtgag gtgcgccgtc gtcagcgagt gcagccagga gagccggtcc gctccggtga
     1021 cggtgaggac gtcacggtgg gacaggtcga cgaccgcgag gccctcggtg agcagccgct
     1081 gctcgcgcat cgggtcgccg tagtgggccg cgacgcgggc gtcggccccc gcaccggcta
     1141 ccgcacccgg gcgggacagc agcgggctca ctcgggcggc cggttcggtc gacatgctgc
     1201 acaccatcca tggattgcca tatgcgtgac gtctgcttca aagccgagcc ggtcgcgcag
     1261 attcccgacg aaggtgctcg cgctggacgc gggcacgctc tcgacctggg cacagccgag
     1321 acagaccagg tggatgtggt tggcatgctc ggccaggtgg tacgacgggg agcggtggtc
     1381 gagatgggtg tgcgacacga tgccgagctc ttcgagcgcc tcgagcgtgc ggtagaccgt
     1441 cgaggcaggg acggggctgc cgccgtcctg cgcgacgaac ccgacgacgt cgtccggtgt
     1501 cgcatgctcc agcgctgcga gcgcgccgat gatccggcgc cgttgcgggg tgaggcgccg
     1561 cccatgggcg cgcagcgtct cgccgagctc ggccatcggg gcgctacccc actcgtttga
     1621 gctgagccga caggtggctc tggagcggct gcccgacagc cgccatgtcc atcacccaca
     1681 tgagcgcgga gtcgaccagg ccgtagacgc ggcgcgcggc ggagtactcc ttggcctggg
     1741 ggctgcgcat gaccccgtcg gtacgcagct cgatcctggc cgggctcacc gggccgtaat
     1801 agagctcgag gatgccggtc gggtggacca gcagcagctc gacctcgcca tcgggtgccg
     1861 ggcgcaggaa gcccagctcg acggccgagc tcttgacccg ggtgcccccc gcgtcgagga
     1921 gccaggcgcg gctctgccat tcgaggaagc ctcgcccgtc atggctgacg acgagttcct
     1981 ggccgaagtg gcgcgactcg atcgtggggt agccgaccac gccggcgccc tcccagcggc
     2041 cgacgagcca ggcgaccggc acgagctccg ccgccaaggt cgggtcgatc gcgaacgcca
     2101 cggctgcgct cctgcccagg tctgcgggac atcttctgac cccgtgatcg tagccttgcc
     2161 gtcatggacg agtcccccgc cggcaccgcc ggcccccgcc cagggcttcg gccgctcgtc
     2221 gtcaaggtca cctgcggggt cgaggcggtc gagcggctga gccaggcgtt cacggtcgcg
     2281 gccacggccg ccctggccgg ggcaccggtc agtgtgtggc tcaccggaga ggccgtgtgg
     2341 ctcgccgtgc ccggacgagc cgaggacgtc gagctcgccc acagcacgcc gctcgccgcc
     2401 ctgcgcgacg acctgctcga cgccgcgcgg atcaccgcgt gcacccagtg cgccgcgcgc
     2461 cgtggcctcg cggcgcagga cctgctgccc ggcatccaga tccggggctc ggcctcgttc
     2521 gtggaggagg tcctcggtgc tcaggcgcag gccctcgtct actgaccacc gggcccggcc
     2581 gcgcctgtca ggtgatgaac accaggtcga cgaggtaggc cagcaccccc acgacgagga
     2641 ccggggccgc ggcgaccgct gccgccccgg cccgggatgc ggcggccggg gggatgacga
     2701 ggacgcggcg ggcggcggcc gaggtcgccg cggcgacggc ccccaccagc gccaacggaa
     2761 gctcggggag cgaggtggtc gcgagagcca cgccgagccc ggcgagcgcg ccgacgatca
     2821 tggcggtgac gacgaggaag ggccgcaggc tctcccaccg ggccagcagg tccacgagga
     2881 cggcgcccgc cacggccagc atcaccgcga gcaccaccgc ggcgccgtgc ctgagcgacc
     2941 cgagggggat catggccacc ccggaggcga cgatggcaac ggcgctgacc gagcccgcca
     3001 cgctttcggt gagccgcggc cggtggtctc cgcggagcag ctggtgggcg aacgacgcgg
     3061 tcgtcgagat ggccagggcg atcggaagcc agcgcagcaa cgggtcgtcg agggtcgccg
     3121 ccgccgccac cacgcaggtg agcgagccga acacggtcac cgccgtcccg ctcctcgggc
     3181 tgggcaccac
//
LOCUS       DATAIX010000471         2579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_174785, whole genome shotgun sequence.
ACCESSION   DATAIX010000471 DATAIX010000000
VERSION     DATAIX010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174785"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1907)
                     /gene="valS"
                     /locus_tag="VHM65_07085"
     CDS             complement(<1..1907)
                     /gene="valS"
                     /locus_tag="VHM65_07085"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF009687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX2705506.1"
                     /translation="MTTHAPASPSRLPDRPTVDGLEEKWGQVWREQGTYRFDRAAALA
                     APREATYAIDTPPPTASGSLHVGHVFSYTHTDAIARFKRMAGFEVFYPMGWDDNGLPT
                     ERRVQNYYGVRGDASLPYVPGFVPPQEGGEGRSTRAADQQPVSRRNFIELCDILTVRD
                     ERTFESLWRHLGLSVDWSINYRTIDGHSRATAQQAFLRNLARGEAYAAEAPGLWDVTF
                     QTAVAQAELEARDYPGHYYRVAFHRGEGEAVWIETTRPELLAACAALIAHPDDERFQG
                     LFGTTVTSPVFGVELPVLAHPGAEMDKGSGIAMCCTFGDLTDVQWWRELRLPTRSVIT
                     RTGRIQGETPEWISSPAGRELFARIAGASTYRARTVVADALRASGDLDGEPTATQRKA
                     NFYEKGDKPLEIVTSRQWYIRNGGRDAELNAALIERGREVEFHPAFMRTRYENWVGGL
                     NGDWLISRQRFFGVPIPVWYPLDASGEPDVEHPIVPAEEALPVDPVAQAPAGYTESQR
                     GLPGGFVGDTDIMDTWATSSLTPQIACGWRGDDELFAKIFPMDLRAQAQDIIRTWLFS
                     TVVRAHLEHGSLPWRHAAISGWILDPDRKKMGKSTGNAETPEGMVGEHGADGVRYWAC
                     SAKLGTDAAFDT"
     gene            complement(1980..>2579)
                     /locus_tag="VHM65_07090"
     CDS             complement(1980..>2579)
                     /locus_tag="VHM65_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705507.1"
                     /translation="MRLWVRLVLFLAFQVVLGALVSGTFGATPRESDQVGDATGPALF
                     VIGVGIFLALVARADGSFPMGFHWLAIGSAVVLLVLLLDQLLRVYRETQLTVVQATVT
                     RSDLLGSGAQDLDVRLPDGSIGSTRFIVDAPLTNDLIEVLADPRGWTHPLEPRQGAGS
                     ANPLLLLIAVQAGAVAQVEGFSALRRARGLYRTRSIWHD"
BASE COUNT          381 a          906 c          933 g          359 t
ORIGIN      
        1 gtgtcgaacg cggcgtcggt cccgagcttg gccgaacatg cccagtagcg cacgccgtcg
       61 gcgccatgct cgcccaccat gccctcgggg gtctcggcgt tgcccgtgga cttgcccatc
      121 ttcttgcggt cagggtcgag gatccagccc gagatcgccg catgcctcca cggcagcgac
      181 ccgtgctcga ggtgcgcccg gacgaccgtc gagaacagcc aggtccggat gatgtcctgg
      241 gcttgcgccc gcaggtccat cgggaagatc ttggcgaaca gctcgtcgtc cccgcgccag
      301 ccgcaggcga tctgcggcgt cagcgacgag gtcgcccacg tgtccatgat gtccgtgtcg
      361 ccgacgaatc cacccggcag gccgcgctgg gactcggtat accccgcggg ggcctgggcc
      421 accgggtcga ccggcaacgc ctcctcggcc ggcacgatcg ggtgctcgac gtccggctca
      481 cccgacgcgt cgagcgggta ccacaccggg atcggcacgc cgaagaaccg ctgcctcgag
      541 atgagccagt cgccgttgag cccgcccacc cagttctcgt agcgcgtccg catgaacgcc
      601 gggtggaact cgacctcccg cccgcgctcg ataagcgccg cattgagctc ggcgtcgcgg
      661 cccccgttgc ggatgtacca ctgccgtgag gtgacgatct ccagcggctt gtcgcccttc
      721 tcgtagaagt tggccttgcg ctgggtcgcc gtgggttcgc cgtcgaggtc accggaggcg
      781 cgcagtgcgt ccgcgaccac cgtccgggcg cggtatgtcg acgcccccgc gatccgcgcg
      841 aagagctccc ggccggcggg gctgctgatc cactcggggg tctcgccctg gatgcggccg
      901 gtgcgggtga tcaccgaccg ggtcggcagc ctcagctcgc gccaccactg gacgtcggtg
      961 aggtcgccga aggtgcagca catggcgatg ccggagccct tgtccatctc ggcgcccggg
     1021 tgggccagca cgggcagctc aaccccgaac accggcgagg tcaccgtcgt gccgaacagc
     1081 ccctggaacc gctcgtcgtc cgggtgcgcg atgagcgccg cgcaggccgc gagcagctcg
     1141 gggcgggtcg tctcgatcca caccgcctcg ccctcgccac ggtggaaggc cacccggtag
     1201 tagtgacccg ggtagtcgcg cgcctcgagc tcggcctgcg ccacggcggt ctggaaggtg
     1261 acatcccaca agcccggcgc ctccgcggca tacgcctcac cgcgggccag gttgcgcagg
     1321 aacgcctgct gggcggttgc ccgggagtgg ccgtcgatgg tgcggtagtt gatgctccag
     1381 tcgaccgaga gcccgaggtg gcgccagagc gactcgaagg tccgctcgtc gcggacggtg
     1441 aggatgtcgc acagctcgat gaagttgcgc cgcgagaccg gctgctggtc ggccgcccgg
     1501 gtcgacctgc cctcgccacc ttcctgcggg ggcacgaagc ccggcacgta gggcagcgac
     1561 gcgtcaccgc ggacgccgta gtagttctgc acccgtcgct cggtcggcag cccgttgtcg
     1621 tcccacccca tcgggtagaa cacctcgaag ccggccatcc gcttgaaccg cgcgatggca
     1681 tcggtgtgcg tgtaggagaa cacgtgcccg acgtgcagcg acccggacgc ggtcggcggg
     1741 ggagtgtcga tcgcgtaggt ggcctcgcgc ggcgcggcga gcgccgcggc ccggtcgaag
     1801 cggtaggtgc cctgctcacg ccatacctgt ccccacttct cctcgagccc gtccacggtg
     1861 ggccggtcgg gaagacgtga gggactggcg ggggcgtggg tggtcatggc gtgaatcctc
     1921 ccagaccggc gcgggtgcac ggcaacctca ttcccgtatg gcgcgcgccc cggccgagct
     1981 cagtcgtgcc agatcgagcg ggtgcggtag agccccctgg cacgtcgcag cgccgagaac
     2041 ccctcgacct gcgcaacggc ccccgcctgc acggcgatga gcagcaggag cgggttggcc
     2101 gagccggctc cctgccgggg ctccagcggg tgggtccagc cgcgcgggtc ggcgaggacc
     2161 tcgatgaggt cgttggtcag cggggcgtcg acgatgaacc gggtcgagcc gatactgccg
     2221 tccgggaggc ggacatcgag gtcctgggcg cccgagccca gcaggtccga gcgggtcacc
     2281 gtggcctgga cgacggtgag ctgggtctcg cggtagaccc gcagcagctg gtcgaggagc
     2341 aggacgagca ggacgaccgc ggacccgatg gccagccagt ggaaccccat ggggaaggac
     2401 ccgtcggccc gggccacgag agcgaggaag atcccgaccc cgatgacgaa cagggcgggc
     2461 ccggtggcgt caccgacctg gtcggactcg cggggcgtgg ctccgaaggt gcccgagacg
     2521 agggctccga gcacgacctg gaacgcgagg aagagcacga gccgcaccca cagccgcat
//
LOCUS       DATAIX010000472         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_85649, whole genome shotgun sequence.
ACCESSION   DATAIX010000472 DATAIX010000000
VERSION     DATAIX010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85649"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..1016
                     /gene="phoU"
                     /locus_tag="VHM65_07095"
     CDS             327..1016
                     /gene="phoU"
                     /locus_tag="VHM65_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX2705508.1"
                     /translation="MRDAFHDELALITDQLVELTRLAGSAISRATTALLDADLALADS
                     VILADEQIDEIRQLLDELSIEILARQQPVATDLRTVVTSMRMSSDLERMGDLARHVAK
                     IARLRYPESAVPPAMRASVVQMGQVATRIVAKTGSIIAARDVKAAQTLDGDDDEMDTL
                     HRELIASMLSGSWPYGTETAVDMTLIGRYYERFADHAVSIANRVVYLVTGSYTHDQTD
                     HDEPGHAGVSV"
     gene            complement(1026..1769)
                     /locus_tag="VHM65_07100"
     CDS             complement(1026..1769)
                     /locus_tag="VHM65_07100"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF010718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HEX2705509.1"
                     /translation="MTYTLVLLRHGESEWNARNLFTGWVDVDLTDKGRAEAVRGGELL
                     KEAGILPDVVHTSLQRRAITTANLALDVADRHWIPVRRDWRLNERHYGALQGKDKKQT
                     LATYGEEQFMLWRRSYDVAPPPIATDDPFAQTGDPRYAGLGGAMPASECLKDVVTRML
                     PYWESAIVPDLLAGQAVLVAAHGNSLRALVKHLDGISDEAIAGLNIPTGMPLIYRLDE
                     ALAPTVAGGEYLDPGAAAAAAAAVANQGR"
     gene            complement(1779..>2150)
                     /locus_tag="VHM65_07105"
     CDS             complement(1779..>2150)
                     /locus_tag="VHM65_07105"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX2705510.1"
                     /translation="STSVSAFVIRNPDENHEAVYRFLLRRNLKLPGLAYAIDASGDIF
                     LRGQLPTSAVDSATLDQLLGVVLEAADAPFNELLALGFLTSMRKEWEWRISRGEPTHN
                     LEAFRHLLQGPAAEAPGAPPD"
BASE COUNT          347 a          764 c          718 g          321 t
ORIGIN      
        1 atcgtcccgg acttgagcag ggcgaggacg tcgaacgcgc cagactccag tggcgcaggt
       61 gtctgggcga gggcacgacc ctctcgcgag cggttggacc gccgtgcgga gacgaacgtg
      121 gtcaggccca agcccaggcc caggatgccg cccaccgccg ccgcagtcag atccacacga
      181 tcagagtacg cactctggtc agggcagatg ccacgatgaa cggcctggcc actccggccg
      241 cgcactgttc acctgcggtg cggggttcgt tcaccccgct ctggcatcgt cgccactgca
      301 gcccacccgc cccagacagg acgcccatgc gcgacgcttt ccacgacgag cttgccctca
      361 tcaccgacca gctcgtcgag ctgacccgcc tcgccgggtc ggcgatctcc cgcgcgacga
      421 ccgcgctgct cgacgccgac ctcgcactgg ccgacagcgt catcctggcc gacgagcaga
      481 tcgacgagat ccgtcagctc ctcgacgagc tctcgatcga gatcctcgcc cgccagcagc
      541 cggtcgccac cgacctgcgc actgtcgtca cgtcgatgcg gatgagctcg gacctcgaac
      601 gcatgggcga cctcgcccgg catgtcgcca agatcgcgcg gctgcgctac cccgagtcgg
      661 ccgtcccgcc ggccatgcgc gcctcagtcg tccagatggg tcaggtggct acccgcatcg
      721 tggccaagac tggctcgatc atcgcggcca gggacgtcaa ggcagcgcaa acccttgacg
      781 gggacgacga cgagatggac accctgcacc gcgagctgat cgcgtcgatg ctcagcgggt
      841 catggcccta cggcaccgaa accgccgtcg acatgacgct gatcgggcgc tactacgaac
      901 gcttcgccga ccacgccgtg tcaatcgcca accgcgtcgt ctacctcgtc accggttcct
      961 acacacacga ccagaccgac cacgacgagc caggacacgc cggggtttca gtctgacccc
     1021 aggggctagc ggccctggtt ggcgactgcg gccgccgcag ccgccgcagc gccggggtcg
     1081 aggtactctc cgccggccac cgtgggggcc agtgcctcgt cgagacggta gatgagcggc
     1141 atacccgtcg ggatgttgag cccggcgatc gcctcgtcac tgatcccgtc gaggtgcttg
     1201 accagggccc gcaggctgtt gccgtgggcg gccacgagca ccgcctgccc cgccagcagg
     1261 tcggggacga tggcggactc ccagtacggc agcatccggg tcacgacatc cttgaggcac
     1321 tccgaggccg gcatggcccc gccgagcccg gcataccgcg ggtccccggt ctgggcgaac
     1381 gggtcgtcag tggcgatcgg cggcggcgcg acgtcatacg agcggcgcca gagcatgaac
     1441 tgctcctcgc cgtacgtcgc gagcgtctgc ttcttgtcct tgccctgcaa ggcgccgtag
     1501 tggcgctcgt tgagccgcca gtcgcggcgc accgggatcc agtgccggtc ggccacgtcg
     1561 agcgcgaggt tggccgtcgt gatggcccgt cgctgcaggg acgtgtggac gacatccggg
     1621 aggattccgg cctccttgag cagctctcca ccgcggaccg cctcggcccg gcccttgtcg
     1681 gtgaggtcga cgtcgaccca gcccgtgaac aggttccggg cgttccattc gctttcaccg
     1741 tgacgcagca gcacgagggt gtaagtcatg ggccaaccct agtccggggg cgcgccaggc
     1801 gcttcggctg caggcccttg gagcagatgg cggaatgcct cgaggttgtg agtcggctca
     1861 ccccgcgaga tccgccattc ccactccttg cgcatcgagg tgaggaagcc cagggcgagc
     1921 agctcgttga agggggcgtc ggccgcctcg aggaccacac cgagcagctg gtcgagggtg
     1981 gcggagtcca ccgccgaggt cgggagctgc cctcgcagaa agatgtcccc cgaggcgtcg
     2041 atggcatagg cgagacccgg cagcttgagg ttgcgccgca gcaggaagcg gtagaccgcc
     2101 tcgtggttct cgtcggggtt gcggatgacg aacgccgaca ccgacgtgct
//
LOCUS       DATAIX010000473         1676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_115225, whole genome shotgun sequence.
ACCESSION   DATAIX010000473 DATAIX010000000
VERSION     DATAIX010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115225"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..809
                     /locus_tag="VHM65_07110"
     CDS             294..809
                     /locus_tag="VHM65_07110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2705511.1"
                     /translation="MTSARGLPPDVEALLTTVYEAESARLVSFARLFVDDRTAAEDLV
                     QEAFIRLARSAHRIRDPSRCASYVRSIVVNLARDHNRRGLVSLRHRPSAAPDERSAEE
                     EGTLRARDAAVIQALRGLPRRQRDCVALRYFHELGIPEIAQTLAISPNSVKTHLSRGL
                     AALARSLGEAP"
     gene            806..1417
                     /locus_tag="VHM65_07115"
     CDS             806..1417
                     /locus_tag="VHM65_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705512.1"
                     /translation="MSALEDRLHEAMAATGRLPAPDLFERVLDGVSADDARRRRRRMA
                     LLAWVPALVAGVLGIALVTPREDGRLMMPWWILELGTNVVLVTIALWLGPFIKRFGRA
                     YAADVFHDNPLTGKSYLVLTDIVYYLIFAAYILFTMVVEPSADWGSDAVTSAQVASSL
                     SRLGGILLVIAVLHGLNIVIMPMLGRLFSLNRRLPARRDPDSS"
     gene            complement(1451..>1676)
                     /locus_tag="VHM65_07120"
     CDS             complement(1451..>1676)
                     /locus_tag="VHM65_07120"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF016438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX2705513.1"
                     /translation="VMGCVVNGPGEAREADLGVASGNGKGQIFVRGEVIKTVPESQIV
                     ETLIEEAMRLADGMGEAADGEAGSPMVTVS"
BASE COUNT          227 a          623 c          555 g          271 t
ORIGIN      
        1 caccaccaca ccaccggcag gcggggcagg cccagggcgt tcgccccggc agcgagtggt
       61 cgcgccgtcc ctgtggccgt cgcgtgcatg gaagtcgtca tgaacccgcc ttcgtcggtc
      121 gggccgccgc ccttgagctg cccggtcatc tcatggacgg ccgggccccc tcctccgggt
      181 gaccgcgaga gccggcagat ctcggccacc gagatctgtc accccgcaga cgcctttcgc
      241 tcgtccacga ggtcagggca ctcttggacc gaccgcgtgg aggggaggcg accgtgacca
      301 gcgcacgtgg cctgcctccc gacgtcgagg cgctcctcac gacggtgtat gaggccgagt
      361 ccgcccggct ggtctccttc gcccgcctct tcgtcgacga ccggaccgcc gcagaggacc
      421 tggtccagga ggcgttcatc cggctcgccc gttcggctca ccggatccgc gacccgtcgc
      481 gctgcgcgtc atacgtccgt tcgatcgtgg tcaacctggc ccgcgaccac aaccgcagag
      541 gcctggtgtc actgcggcat cgaccgtccg cggcgcccga cgagcggtcg gccgaggagg
      601 aaggaaccct gcgtgcccgt gacgcggcgg tcatccaggc gctgcgcggc ctccccagga
      661 gacaacggga ctgcgtcgcc ctgcgctact tccacgagct gggcatcccc gagatcgccc
      721 agacccttgc catctcgccg aactcggtga agacccacct gtcgcgcggc ctggccgcgc
      781 tggcccgcag cctcggggag gcgccatgag tgcactcgaa gaccggctcc acgaagccat
      841 ggccgccacc gggcggctcc ccgcacccga cctgttcgag cgtgtcctcg acggcgtgtc
      901 cgcggacgac gcccggcgac gacgtcggcg tatggcgctg ctggcctggg tgccggccct
      961 ggttgccggc gtgctcggca ttgccctggt gaccccacga gaggacggaa gactgatgat
     1021 gccctggtgg atcctcgagc ttggcacgaa cgtggtgctg gtgacgatcg cgctctggct
     1081 cgggccgttc atcaaacggt tcggccgtgc ctatgccgcg gacgtcttcc acgacaaccc
     1141 gctcaccggc aagagctacc tcgtcctgac cgacatcgtc tactacctca tcttcgccgc
     1201 atacatcctg ttcacgatgg tggtggagcc gtcagcggac tgggggtccg acgcggtgac
     1261 cagtgctcag gtggcgtcgt cgctgagccg gctcggcggc atcctgctgg tcatcgccgt
     1321 cctgcatggg ctcaacatcg tcatcatgcc gatgctcggg cggttgttct cgctcaaccg
     1381 ccgcctcccc gcgcgccgcg accccgactc gtcctgagcg ggccggagcc gagcgggcct
     1441 ggatgggcgg tcagctgacc gtgaccatgg gcgagcccgc ctcgccgtcg gccgcctcgc
     1501 ccatcccgtc ggcgaggcgc atggcctcct cgatgagggt ctcgacgatc tgcgactccg
     1561 ggacggtctt gatgacctcg ccgcggacga agatctggcc cttgccgttg ccgctggcga
     1621 cgccgaggtc ggcttcgcga gcctcgcccg ggccgttgac gacgcagccc atgacc
//
LOCUS       DATAIX010000474         2507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_85774, whole genome shotgun sequence.
ACCESSION   DATAIX010000474 DATAIX010000000
VERSION     DATAIX010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85774"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VHM65_07125"
     CDS             complement(<1..468)
                     /locus_tag="VHM65_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705514.1"
                     /translation="MPGEETSSTLVVQVKGQVLSAEVAAKLTSATIDDTLGVPDMFVL
                     RFLDDGASVLEQAKVTIGAEVKLVVQRSGGGAPTPLLTGEVTAVEIELTPGGTRTVVR
                     GYDKSHRLFRGGRVLSFVNQKASDIARKTAANAGLAAGTINADGGVLQHVSQDG"
     gene            complement(458..1297)
                     /locus_tag="VHM65_07130"
     CDS             complement(458..1297)
                     /locus_tag="VHM65_07130"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705515.1"
                     /translation="MAPSPVAFTATGSPGGAGSTAGGRPKLEHAYLQLFEPSPDGSLA
                     KPGAEIGKIDFQFNPKELSLSKSAKWARETGKGNKKSGPPQYQGPQPSKLSLEMFFDA
                     SDTQDDSVVKRVEKLFACCVPTATSHDQKKGSPPWVLFRWGGLTGFLAYLSSVTAKYT
                     VFTSGGLPVRAVCTVQLDELAGETPRQNPTSGGLVPRRVHVLVDGDTLQGIAYQEYGD
                     ASMWRAVARANGIDDPLRLTSGRTVLLPTPEDLVSGPVTPGHEMYDADPAVRHTAEVS
                     GAR"
     gene            complement(1303..1743)
                     /locus_tag="VHM65_07135"
     CDS             complement(1303..1743)
                     /locus_tag="VHM65_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX2705516.1"
                     /translation="MILEDVNTALSVAFVVKLDNESLGMFTSCDGLGCEVVMETREEG
                     GNNSFVWQLPTRLKYPNVKLTRPLGRDTEKIAKWFASMATGYKRCTGTIEAMRADGTV
                     IATWGLIDVVPVRWSGPSLSADSPKVLTETVEIAHHGFIAAGKG"
     gene            complement(1757..>2507)
                     /locus_tag="VHM65_07140"
     CDS             complement(1757..>2507)
                     /locus_tag="VHM65_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705517.1"
                     /translation="FVPTEPGVPASAANSGPDPTTSRRLGQLPSLRVLPLTRAVDTAA
                     MSSAVPVQRVVGSATGPALAHAATGPARPRVADTVASWPVRALPTRVGPVDAPTTPEG
                     AVVQRLPLAAPTAPPGLATAADDDLGHVGASAHVSAPGVLEPLDLPLGVSTSAPYAHQ
                     VQRTESPQEAETERLVEVHVPVPTPVAASAAPGDPAVQRVGGAADPAAPIGSSDEVDE
                     LARRLFDPLMLRLRAELLVDRERRGFRTDAW"
BASE COUNT          342 a          864 c          911 g          390 t
ORIGIN      
        1 gccatcctgg gacacgtgct gaaggacgcc accgtcggcg ttgatggtgc ccgcagcgag
       61 tccggcgttg gctgcggtct tgcgggcgat atcggaggcc ttctggttga cgaacgacag
      121 gacccggccg cctcggaaca gccggtggga cttgtcgtag ccgcgcacga cggttcgcgt
      181 gccaccgggg gtcagctcga tctcgaccgc ggtcacctcg cccgtgagca agggggtggg
      241 tgcgccaccg ccactgcgct ggacgacgag cttgacctcg gcgccgatcg tcactttcgc
      301 ctgctccagc accgacgctc cgtcgtcgag gaagcgcagc acgaacatgt ccggcacacc
      361 caaggtgtcg tcgatcgtgg cgctggtcag cttcgccgcg acttccgcgg acaggacctg
      421 gcccttgacc tggacgacga gggtgctgct ggtctcctca ccgggcaccg gacacctccg
      481 cggtatgccg gaccgccggg tccgcgtcgt acatctcgtg cccaggggtg acgggcccgc
      541 tcacgaggtc ctcgggggtc ggcaggagga ccgtgcgccc cgaggtgagg cgcagggggt
      601 catcgatccc gttggcccgg gcgaccgccc gccacatcga cgcgtcgcca tactcctggt
      661 aggcgatccc ctgcagggtg tcgccgtcga cgagcacatg gacccgacgc gggaccaggc
      721 ctccggatgt ggggttctgc cggggggtct cgccggcgag ctcgtcgagc tgcacagtgc
      781 agaccgctcg gaccggcagg ccgccggagg tgaagaccgt gtacttcgcc gtgacactgc
      841 tcaggtaggc caggaagccg gtgagaccgc cccagcggaa gaggacccag gggggtgaac
      901 ccttcttctg gtcgtgggac gtggccgtcg ggacacagca ggcgaacagc ttctcgaccc
      961 gtttgacgac actgtcgtcc tgcgtgtccg aggcgtcgaa gaacatctcg aggctgagct
     1021 tggacggctg cggcccttgg tactgggggg gcccgctctt cttgttgccc ttgccggtct
     1081 cgcgcgccca cttggctgac ttgctcaggc tgagctcctt ggggttgaac tggaagtcga
     1141 tcttgccgat ctcggcgccc ggcttggcca gcgacccgtc cggcgagggc tcgaagagct
     1201 ggaggtaggc gtgctcgagc ttgggccgac cacccgcggt ggagccggcc ccgcctgggg
     1261 aacctgtggc ggtgaacgca accgggctgg gcgccatacc gctcacccct tccctgcggc
     1321 gatgaacccg tggtgggcga tctcgaccgt ctcggtgagc actttcgggg agtcggccga
     1381 cagcgacggc ccggaccagc ggaccggcac cacgtcgatg agcccccagg tcgcgatgac
     1441 cgtgccgtcc gcgcgcatcg cctcgatcgt gccggtgcac cgcttgtagc cggtcgccat
     1501 gctggcgaac cacttcgcga tcttctccgt gtcccgcccc aagggcctgg tcagcttgac
     1561 gttggggtac ttgagcctgg tcggcagctg ccagacgaac gagttgttgc cgccctcctc
     1621 ccgagtctcc atgacgacct cgcagccgag cccgtcgcag ctggtgaaca tgccgaggct
     1681 ctcgttgtcg agcttgacga cgaacgcgac ggacagcgcg gtgttgacgt cctccaggat
     1741 catggtcggc cccctctcac caggcgtcgg tccggaagcc gcgccgctcg cggtccacga
     1801 ggagctccgc gcgcaaccgg agcatgagcg ggtcgaacag cctgcgggcg agctcgtcga
     1861 cctcgtcgga ggagccgatc ggcgctgccg ggtcggccgc ccccccgacg cgctggacgg
     1921 ccgggtcacc gggtgccgcg ctcgcggcga ccggggtggg gactggcacg tgcacctcga
     1981 cgagacgctc cgtctcggct tcctgcggcg actccgtgcg ctgaacttga tgcgcgtatg
     2041 gcgcgctcgt ggacaccccg agggggaggt cgagcggctc tagcacgccc ggagcgctga
     2101 cgtgcgcgct cgcgccgacg tgaccgaggt catcgtccgc agccgtcgcc aggcccggtg
     2161 gtgcggtcgg cgcggccagc ggtaggcgct gcacgacagc cccctcgggc gtggtgggcg
     2221 cgtcgaccgg gccgaccctc gtcgggagag ccctgacggg ccagctggcc acggtgtcgg
     2281 cgacgcgtgg gcgagcgggg ccggttgcgg cgtgagccag cgccggaccc gtcgccgagc
     2341 ccacgactcg ctggaccggc acagcgctgg acatcgcggc ggtgtcgacc gctcgcgtca
     2401 ggggcaggac gcgcagcgac ggcagctgcc cgagccgccg gctcgtggtc gggtccgggc
     2461 ctgagttcgc agcgctcgct gggacgcccg gctcggtcgg tacgaac
//
LOCUS       DATAIX010000475         2969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_25404, whole genome shotgun sequence.
ACCESSION   DATAIX010000475 DATAIX010000000
VERSION     DATAIX010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25404"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VHM65_07145"
     CDS             <1..190
                     /locus_tag="VHM65_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705518.1"
                     /translation="VSAALEEFGDRWDRGMNNLAGDVEEMAGRLGKIAMTYAELDTSG
                     AERMSALAGSMRTVRVLG"
     gene            complement(474..1178)
                     /locus_tag="VHM65_07150"
     CDS             complement(474..1178)
                     /locus_tag="VHM65_07150"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX2705519.1"
                     /translation="MSRFVPATPAQVAALALALCFATLVSVELRTQLALRRRWTWPVL
                     AVAAAVGVGSMGSAYTTAPLRALYDGGVRTVAILALVAAGLAGLLVLIRRSPSQALLM
                     AATWFTVAAVSWVPTDTAPHLRSYAGTELRLCLTSSSRYTTFGGQIGLWADMVPNAVL
                     YVPFGIAAMLLVGRRVWAALLVVMGTSVFTEVWQATMTDRVCAPNDVMTNTLGGLLGV
                     ASIALLGLARRPVRPA"
     gene            complement(1386..2366)
                     /locus_tag="VHM65_07155"
     CDS             complement(1386..2366)
                     /locus_tag="VHM65_07155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEX2705520.1"
                     /translation="MKAITIPAPGDVDALVLAEVPAPEAADDEVVVDVVAAGVNRADI
                     MQRKGFYPPPPGVSALPGLEVSGRVRVVGPAVRGWSVGDEVCALLDGGGYAEQVAVPA
                     TQLLPVPRGVSLVDAAALPEVACTVWSNLVMTAGLSAGHTLLVHGGSSGIGTMAIQIG
                     RELGVRVAVTAGSADKLEACRTLGAQICINYRDEDFVARVQAGTDGHGADVILDNMGA
                     KYLARNVAALADGGRLVVIGLMGGRVAELDLGVLLSKRAGVIATSLRSRTRESKAAIV
                     ATVREHVWPLVDDGHIHPVVHSRHPLADAAAAHREMEASTHVGKILLLTT"
     gene            complement(2363..>2969)
                     /locus_tag="VHM65_07160"
     CDS             complement(2363..>2969)
                     /locus_tag="VHM65_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX2705521.1"
                     /translation="STVVGCIVLAGGTSRRHGGADKTRLVLGGSTVLDTLLGRLPAAW
                     PVVVVGEERPTNRTVSWTREAPGYGGPLAGIAAGLVALALTTATPTARQPLDGGITVV
                     VAGDMPFAGAAAAALAEALAQAAAPLDAVMACDGDGRDQPLLAAYRTDRLRAAIPADP
                     QGKAVRPVLRTLTALAMPVDERSLLDIDTPEALARARRLAP"
BASE COUNT          439 a         1019 c         1135 g          376 t
ORIGIN      
        1 cgtctcggcc gcgctcgagg agttcggcga ccgctgggac cgcggaatga acaacctggc
       61 tggggacgtc gaagagatgg cggggcggct cggcaagatc gcgatgacct acgccgagct
      121 cgacacgtcc ggggcggagc ggatgtccgc gctggccggg tcgatgcgca ccgtccgggt
      181 gctcggctag cccagggcga agccgtcatg gggacccagg cggccgatcc cgcttcggac
      241 agatcgggaa gtccgctggt gcggagtagg ccgacgactt cgccgggtgg gtcgacaaca
      301 caatcgcgta ccggcaggac cggtccttcg gagccgaccg gttgggaccc ggcggggctg
      361 ggcaggtgtg ccagaaagcg tcgtcaacct ggttgcgggg ttcggcaacc aggttgggcg
      421 cgcgcgtcat ccacccgagc tcggcgctcg cccggtcagg ccggtcaggc cggtcaggcc
      481 ggcctgaccg gccgccgcgc gagacccagc agggcgatcg acgcgacgcc gaggagcccg
      541 ccgagcgtgt tggtcatgac gtcgttcggc gcgcagaccc ggtcggtcat cgtggcctgc
      601 cacacctcgg tgaacaccga tgtccccatg acgacgagca gggcggccca gacgcgccgc
      661 ccgacgagca gcatcgcggc gatcccgaac gggacgtaga ggacggcgtt ggggaccatg
      721 tccgcccaca gcccgatctg cccgccgaag gtcgtatagc gcgaggagct ggtcaggcac
      781 aggcgcagct cggtcccggc atacgagcgc aggtgtggcg cggtgtcggt cggcacccag
      841 ctcaccgccg cgacggtgaa ccacgtggcg gccatgagca gcgcctggct cggactgcgc
      901 cggatgagga cgagcaagcc cgcaaggccc gcggcaacaa gggcgaggat ggccaccgtc
      961 cggacaccac cgtcatagag cgcccgcaga ggggccgtcg tgtaggcgct ccccatcgag
     1021 ccgacaccga ccgcggcggc caccgcgagc accggccagg tccagcggcg gcgcagcgcg
     1081 agctgggttc gcagctcgac cgacacaaga gtggcgaagc acagcgccag ggcgagtgcg
     1141 gcgacctggg caggcgtcgc cgggacgaag cgggacaagg gacacctcct gcggagccca
     1201 acgccgggtc gggaccgttc gtgccggtgt cggcggcccc gggcgctcca gcccgtccct
     1261 gctcgcccgg cctaggcatc ctcgcgccgg atagtggccc tgcgcgccgg gaatgtgcgg
     1321 cgcggggagt gactcagcgg cgcgcggatg tggtggggct gggtatcgac accccaatgc
     1381 ctcggctagg tcgtgaggag caggatcttg cccacgtgtg tcgacgcctc catctcgcgg
     1441 tgggcggcgg cggcgtcagc gagcgggtgg cgcgagtgca cgaccgggtg gatgtgaccg
     1501 tcatcgacga gcggccagac atgctcgcga acagtcgcca cgatggctgc tttggactcg
     1561 cgggtccgcg agcgcagcga ggtcgcgatg acacccgccc gtttggacag cagtacgccg
     1621 aggtcgagct cggcgacgcg cccgcccatg aggccgatga cgacgagcct gcccccgtcg
     1681 gcgagtgcgg cgacgttgcg ggcgaggtac ttggcgccca tgttgtcgag gatgacgtcg
     1741 gccccgtgcc cgtcggtccc ggcctggacc cgggcaacga agtcctcgtc gcggtagttg
     1801 atgcagatct gcgcaccgag ggtccggcat gcctcgagct tgtccgccga cccggccgtg
     1861 accgcgaccc ggacgccgag ctcgcggccg atctggatcg ccatggtgcc gatgccggac
     1921 gagccgccgt ggacgagcag ggtgtgccca gccgagagcc cggcggtcat gacgaggttc
     1981 gaccagaccg tgcaagcgac ctcgggcagg gctgcggcat cgacgaggga cacccctcgc
     2041 ggcaccggca ggagttgggt cgctgggacc gccacctgct cggcataccc gccgccgtcg
     2101 agcagggcac agacctcgtc acccaccgac cacccacgaa cggccggccc cacgactcgg
     2161 actcggccgc tgacctcgag cccgggcagc gcagacacgc cgggtggtgg cgggtagaac
     2221 cccttgcgct gcatgatgtc cgcccggttg accccggctg ccacgacgtc gacgacgacc
     2281 tcgtcgtcgg cggcttcggg ggccgggacc tcggcgagca cgagtgcgtc gacgtcgccg
     2341 ggagcgggga tcgtgatggc cttcatggcg cgagcctacg ggcgcgggcg agtgcctcgg
     2401 gggtgtcgat gtcgaggagg gaccgctcgt caacgggcat ggccagtgcg gtgagggtgc
     2461 gcaggactgg tctgacggcc ttgccctgcg ggtctgccgg gatcgccgcc cgcaggcggt
     2521 cggtccggta tgccgccagc aggggttggt ctcggccgtc accgtcgcag gccatcacgg
     2581 cgtcgagggg ggcggcggcc tgggcgaggg cctcggccag cgctgcggcg gccgctccgg
     2641 cgaagggcat gtccccggcg accacgacgg tgatgccgcc atcgagcggc tgccgggctg
     2701 tcggcgtggc cgtggtcaag gccagggcca cgagccctgc cgcgatcccg gccagcgggc
     2761 cgccataccc gggggcctcg cgggtccagc tgaccgtgcg gttcgtgggg cgctcctcac
     2821 cgacgaccac gaccggccag gcggccggaa ggcgcccgag cagggtgtcg aggaccgtgc
     2881 tgcccccgag gacgagtcgc gtcttgtcag cgccgccgtg gcgccgggag gtccctccgg
     2941 ccaggacgat gcagccgacc accgtgctc
//
LOCUS       DATAIX010000476         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_189960, whole genome shotgun sequence.
ACCESSION   DATAIX010000476 DATAIX010000000
VERSION     DATAIX010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189960"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VHM65_07165"
     CDS             complement(<1..763)
                     /locus_tag="VHM65_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705522.1"
                     /translation="MDTRPRRRMPTRHSAPLAVLLAVLVAGCAVDPTTSATASATSTG
                     APPAVTSSRAPSTTMPSAAMPTVSPHPSPASLGDFRLAVAPDAATAWTGINWRKLVTD
                     DPLAQVRSVVRWRGGFVAVGEPIATGATGATSRTPVWVSTDGADWRLLDTEVFGPATI
                     VVGLGETAEGIVALTLQGGADQCQGQDTRPNCWMPVAPLQSWTSSNGTTWTAHAGPIG
                     FGLPMPGCYECGVTIPIVRSGTPGLLVVNYSGTEAG"
     gene            complement(769..>1634)
                     /locus_tag="VHM65_07170"
     CDS             complement(769..>1634)
                     /locus_tag="VHM65_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705523.1"
                     /translation="LGAALVTPAGLHEVPAVDTGSWAGQVVAGPAGLLWVLGADRSAS
                     CVSAWRYVDDGWRPLEGPQPDMTCLNAAGPTILGSAAMPDGMVIIGMLGADSDRVAWL
                     VRDPGNPPTGTVAGGAIPTPPAEAIPDPLAVKMQHPATCPSLPTTMTAVLDLLPETAV
                     GCFGDQTITIRVWVRDPGEGYGGTCGPFTPAWIRECVLPDYLLTDGPPGATADVPTLH
                     AMRLPKAKGDLVGVGRWVKVQGHYDDPVSPSCRAAGEVAMIGLEPEQPRAMDVSACRL
                     VFVVTDIRTVR"
BASE COUNT          263 a          556 c          584 g          231 t
ORIGIN      
        1 ctccagcctc ggtcccggaa tagttgacga ccagaaggcc gggagtccca gaccggacga
       61 tcgggatggt gacgccgcac tcgtaacacc ccggcatcgg aagaccgaaa ccaatcggtc
      121 ccgcatgggc tgtccaggtc gtgccgttgg aggaggtcca cgactgcaac ggtgcgacgg
      181 gcatccagca attcggccgg gtgtcctggc cttggcattg atccgcgccg ccctggaggg
      241 tcagcgcgac gatgccctcg gccgtctcgc cgagtccgac cacgatggtc gccggaccga
      301 agacttcggt gtcgaggagg cgccagtccg cgccatcggt ggacacccac actggcgtcc
      361 tcgaggtcgc accggtcgca cccgttgcga tcggctcgcc gacggcgacg aacccacccc
      421 gccagcggac cacggatcgg acctgcgcga gcgggtcatc agtcacgagc ttgcgccagt
      481 tgatgccggt ccaggccgta gcggcatcgg gcgcgacggc taacctgaag tccccgaggc
      541 tggccggcga cgggtggggc gagaccgtcg gcatggctgc cgacggcatg gtggtggacg
      601 gggctcgtga tgaggtcaca gccggtggag cgccggtcga ggtcgcgctg gcggtcgcgc
      661 tggtggtcgg gtcgacggca cagcctgcga cgagaacggc aagcagaacc gccaacgggg
      721 cggaatggcg ggtcggcatg cgacgccggg gacgcgtgtc catgtcgctc accggaccgt
      781 ccggatgtcc gtgaccacga acaccaggcg acaggcggag acgtccatcg cacgcggctg
      841 ctcgggctcg aggccgatca tggcgacttc gccggctgcg cggcatgatg gcgagaccgg
      901 gtcgtcgtag tgaccttgca ccttgaccca gcggccaacg ccgacgaggt cgccctttgc
      961 ctttggcaac cgcatggcgt gcaatgtcgg gacgtctgcc gtggccccgg gaggcccgtc
     1021 ggtcagcagg tagtccggca agacgcactc ccggatccag gccggggtaa acgggccaca
     1081 cgttcctccg tagccctctc ctgggtcccg cacccatacc cggatcgtga tcgtctggtc
     1141 gccgaagcag ccgaccgcag tctcgggcaa gaggtcgagc accgccgtca tcgtcgtcgg
     1201 taacgaaggg caggtggccg ggtgctgcat cttgaccgcg agcggatccg ggatcgcttc
     1261 tgcgggtggc gtcgggatcg cgccacctgc cacagtcccg gtcggcgggt tccccgggtc
     1321 tcggacgagc cacgcgacac ggtcgctatc ggcccccagc atgccgatga tcaccatgcc
     1381 gtcgggcatc gccgcggagc cgagtatggt ggggccggcc gcgttgagac aggtcatgtc
     1441 gggttgcggg ccttcgagcg gacgccaccc atcgtcaacg tagcgccagg ccgacacgca
     1501 gcttgccgag cggtccgccc cgaggaccca cagcagaccg gccggccccg ccacgacctg
     1561 accggcccac gacccggtat cgacggctgg gacctcgtgg agtccggcag gagtgacgag
     1621 ggcggcgccc aggc
//
LOCUS       DATAIX010000477         1802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_279468, whole genome shotgun sequence.
ACCESSION   DATAIX010000477 DATAIX010000000
VERSION     DATAIX010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279468"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1802
                     /locus_tag="VHM65_07175"
     CDS             <1..>1802
                     /locus_tag="VHM65_07175"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2705524.1"
                     /translation="YGAVNRTRAPRADSGPDRPARSVGPDRASRPEWDARPSWDAGRD
                     AEAQRLEADTWVSARSTKHVPEADGARPARVGADNGFATLGLPTLIVERLAREGITTP
                     FPIQAATIPDALAGRDVLGRGQTGSGKTLAFGLPTLVRLADEGVRPAPRRPRALILVP
                     TRELAMQVSDSLEPLAHVLGLRHKLVAGGLSYTTQISALNRGVDLLIATPGRLIDLIE
                     REAVNLGDVRIAILDEADHMADMGFMPEVTTIMDTIPAGGQRLLFSATLDKGVDEIVA
                     RYLTDPVTHSTDDVTASVTTMEHYVLLIDPQHKKALTAEVANRLGRTLVFVRTKLGAD
                     RVAMQLREQGVLAAALHGGLNQGARNRVLGAFRTGTLPVLVATDVAARGIHVDDVAVV
                     LQADPPQDHRDVVDVDTARGDIGGDEDGERAGAEGPEDAVARALVEAAVQRGRQDALL
                     AQLHRDPVGAELGADEDNRPTEPVGDLGGKGLLVLRVDEQDVVLHRGHRGGDVVGRVG
                     HRVGQVARDDLVDALVQRCREEQPLTTGGDLVHDRRNLGHEAHVSHMVGLVEDGDAHV
                     PEVDSLALDEVDGPTGRRDEQVDPAVQCRDLGRV"
BASE COUNT          260 a          654 c          628 g          260 t
ORIGIN      
        1 gtatggcgcc gtgaaccgga cgcgcgcgcc gcgggcggac tccggcccgg accgtcccgc
       61 acgcagcgtc ggcccagacc gagcaagccg tcccgagtgg gacgctcgcc cttcgtggga
      121 tgccggccgg gacgccgagg cccagcgcct cgaggcagac acctgggtgt cggcccgctc
      181 gaccaagcac gtgcccgagg ccgacggcgc aaggccggcc cgagtcggcg cggacaacgg
      241 tttcgccacc ctcgggctgc cgaccctgat cgtcgagcgg ctggcgcgcg agggcatcac
      301 gacgccgttc ccgatccagg ctgccaccat ccccgacgcc ctcgccggcc gggacgtgct
      361 cggccgtggc cagaccgggt caggcaagac cctcgccttc ggccttccga cgctcgtgcg
      421 gctcgcggac gagggggtcc ggccggcgcc tcgccgcccg cgcgccctca tcctcgtgcc
      481 gacccgtgag ctcgcgatgc aggtgtcgga ctccctcgaa ccgctggccc acgtcctcgg
      541 cctgcggcac aagctcgtcg ccggtgggct gtcctacacg acccagatct cggcactgaa
      601 ccgcggggtc gacctgctca tcgcgacgcc cggtcggctc atcgacctga tcgagcgtga
      661 ggcggtcaac ctcggggacg tgcgcatcgc catcctcgac gaggccgacc acatggctga
      721 catgggcttc atgcccgagg tcacgacgat catggacacc atccccgccg gcggtcagcg
      781 gctcctcttc tcggccacgt tggacaaggg cgtcgacgag atcgtggcgc gctacctcac
      841 cgacccggtg acgcattcga ccgacgacgt caccgcctcg gtgaccacga tggagcacta
      901 cgtcctgctc atcgacccgc agcacaagaa ggccctcacc gccgaggtcg ccaaccggct
      961 cggccggacg cttgtcttcg tccgcaccaa gctgggcgcc gaccgggtcg cgatgcagtt
     1021 gcgggagcag ggggtccttg cggccgcgtt gcacgggggc ctcaaccagg gcgcgcgcaa
     1081 ccgcgtcctc ggggccttcc gtaccggcac cctccccgtc ctcgtcgcga ccgacgtcgc
     1141 cgcccgcggt atccacgtcg acgacgtcgc ggtggtcctg caggccgacc cgccgcagga
     1201 ccaccgagac gtcgtcgacg tggataccgc gcgcggcgac atcggtggcg acgaggacgg
     1261 ggagcgtgcc ggtgcggaag gccccgagga cgcggttgcg cgcgccctgg ttgaggccgc
     1321 cgtgcagcgc ggccgccagg acgccctgct cgcgcagctg catcgcgacc cggtcggcgc
     1381 cgagcttggt gcggacgaag acaatcgtcc gaccgagccg gttggcgacc tcggcggtaa
     1441 gggccttctt gtgctgcggg tcgatgagca ggacgtggtg ctccatcgtg gtcaccgagg
     1501 cggtgacgtc gtcggtcgag tgggtcaccg ggtcggtcag gtagcgcgag acgatctcgt
     1561 cgacgccctt gtccaacgtt gccgagaaga gcagccgctg accaccggcg gggatcttgt
     1621 ccatgatcgt cgtaacctcg ggcatgaagc ccatgtcagc catatggtcg gcctcgtcga
     1681 ggatggcgat gcgcacgtcc ccgaggttga cagcctcgcg ctcgatgagg tcgatgggcc
     1741 gaccgggcgt cgcgatgagc aggtcgaccc cgcggttcag tgccgagatc tgggtcgtgt
     1801 ag
//
LOCUS       DATAIX010000478         3421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_249597, whole genome shotgun sequence.
ACCESSION   DATAIX010000478 DATAIX010000000
VERSION     DATAIX010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249597"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2811
                     /gene="gcvP"
                     /locus_tag="VHM65_07180"
     CDS             <1..2811
                     /gene="gcvP"
                     /locus_tag="VHM65_07180"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEX2705525.1"
                     /translation="MLHTVGQPSLDALVGTAIPPDIRMKAPLDLPAAPSETAVLAELR
                     SYADRNRVMRSMIGMGYYGTVTPGVIARNVLENPGWYTSYTPYQPEISQGRLEALLNF
                     QTMVSDLTGLPIAGASLLDEATAAAEAMTLMRRVGKAAEGAVLLVDAHVFPQTLAVIR
                     TRARPLGITVKVADLRAVSTADGLRAATGGQDVFGVVVQYPAAGGAICDPRPLVEAAH
                     ASGALVTAATDLLALTLLTPPGEWGADIAVGSSQRLGVPMGFGGPHAGFMSVTAPLAR
                     SLPGRLVGVSVDAAGRPAYRLALQTREQHIRREKATSNICTAQVLLAVMASMYAVYHG
                     PTGLAAIARSVHGHAEALRDRLRAGGLAVAEGDLFDTVTVTVPAGADAAVAACAERGV
                     NIWKVDAGSVSLSTDETTTPADLDLVSRALGGAADDPAPVLAAEPAWAAWAVRTSPYL
                     THPVFHRHHSETAMMRYLRRLADRDFALDRGMIPLGSCTMKLNAVTEMAAVSWPEFAD
                     LHPFAPADQNEGIRALIGELSSWLVAITGYDAVSLQPNAGSQGEFTGLLAIHAFHEAN
                     GEGHRRVCLIPSSAHGTNAASAVMAGLRVIVVKSAPGGDIDLDDLRAKVAEHGENLAA
                     VMVTYPSTHGVFEETIAELCALVHDAGGQVYVDGANLNALVGLAQPGRFGADVSHLNL
                     HKTFCIPHGGGGPGVGPVGVRAHLAPYLPNHPLTPSAGPATGVGPVSAAPFGSAGILP
                     ISWAYIRLMGGAGLTEATKVAILNANYVAARLREHYPVLFSGPGGLVAHECILDLRAI
                     TRETGVTVDDVAKRLIDYGFHAPTTSFPVAGTLMVEPTESEDLGELDRFCEAMIAIRA
                     EIEAVHNGVVSAADSVLRHAPHTAESLAGEWDHPYDRLAAAFPPGVEPTEKYWPPVRR
                     VDNAYGDRHLVCSCPPPEAFQE"
     gene            complement(2815..>3421)
                     /locus_tag="VHM65_07185"
     CDS             complement(2815..>3421)
                     /locus_tag="VHM65_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HEX2705526.1"
                     /translation="IEGLLDSLGPRQSEYAVVSELAEVAAGTGVNNSRRMRLSAYVLA
                     GRLEEIVALANERLVRMGEGRYTLGHTDSRAARGARSGLGFVVKDAWTGQTRETSTLS
                     GGEAFMASLALALGLGDAVRAQAGGFDLQTLFVDEGFGSLDEDSLEQVMDVLDSLREG
                     GRAVGIVSHVGELRARIPAQVLVRKSETGSAIEVKGTSAAA"
BASE COUNT          478 a         1274 c         1181 g          488 t
ORIGIN      
        1 atgctgcaca ccgtcgggca accgagcctc gacgcccttg tgggtacggc gatcccgccc
       61 gacatccgga tgaaggcccc gctcgacctg ccggctgcgc cctccgagac ggccgtgctc
      121 gccgagctgc gctcctacgc cgaccgcaac cgggtcatgc gctcgatgat cgggatgggc
      181 tactacggca cggtcacccc gggcgtcatt gcccgcaacg tcctggagaa cccaggctgg
      241 tacacctcgt acacgcccta ccagccggag atctcccagg gccggctcga ggccttgctc
      301 aacttccaga cgatggtctc cgatctcacc ggtctgccga tcgccggagc atcactgctc
      361 gacgaggcca cggcggcggc cgaggcgatg acgttgatgc gccgggtggg caaggcggcg
      421 gaaggggccg tgctgctcgt cgacgcgcac gtgttcccgc agacgctggc ggtgatccgc
      481 acccgtgcgc ggcccctcgg gatcaccgtc aaggtcgccg acctgcgcgc cgtgagcacg
      541 gctgatggac tgagagcggc aacgggaggc caggatgtct tcggcgtcgt cgtccagtac
      601 cccgcggccg gcggggccat ctgcgacccg cgcccgctcg tcgaggccgc ccacgcgagc
      661 ggcgccctgg tgactgcggc caccgacctg ctcgccctca ctctgctgac cccgcccggg
      721 gagtggggcg ccgacatcgc ggtcgggtcc tcccagcggc tcggagtacc catgggcttc
      781 gggggtccgc acgccgggtt catgagcgtc accgcgccgc tggcccggtc gctgcccggc
      841 cgtctcgtcg gggtgtcggt cgatgctgcc gggcgtccgg cataccggct cgccctgcag
      901 acccgcgaac agcacatccg ccgggagaag gcgacctcga acatctgcac cgcgcaggtc
      961 ctgctcgcgg tcatggcgag catgtatgcc gtgtaccacg gccccacggg tctggccgcg
     1021 atcgcccggt cggtgcacgg gcacgccgag gcgctgcggg accggctgcg cgccggcggg
     1081 ctggccgtcg ccgagggcga cctgttcgac accgtcaccg tcaccgtgcc tgcgggcgct
     1141 gacgctgcgg tggccgcctg cgccgagcga ggcgtcaaca tctggaaggt cgacgccggc
     1201 tcggtgtccc tgagcacgga cgagaccacc acacccgccg acctcgacct cgtctcgagg
     1261 gcacttggcg gcgctgcgga cgacccggca ccggtgctgg ccgccgagcc agcgtgggcg
     1321 gcctgggccg tgcggacgag cccctacctg acccacccgg tcttccaccg ccaccacagc
     1381 gagacggcga tgatgcgcta tctgcgccgc ctcgccgacc gggacttcgc gctcgaccgg
     1441 gggatgatcc cgctcgggtc ctgcacgatg aagctcaatg cggtgaccga gatggccgcg
     1501 gtctcctggc ccgagttcgc ggatctgcac cccttcgctc cggccgacca gaacgagggc
     1561 atccgggccc tcatcggtga gctgagcagc tggctcgtgg cgatcaccgg gtacgacgcc
     1621 gtgtcgctgc agcccaacgc cggctcacag ggcgagttca ccgggctcct ggccatccat
     1681 gccttccacg aggccaacgg cgaggggcac cgccgggtct gcctcatccc gtcgagcgcg
     1741 cacggcacga acgcggcgag cgccgtcatg gcgggcctgc gggtcatcgt cgtcaagagc
     1801 gcgccaggcg gcgacatcga cctggacgac ctgcgggcca aggtcgccga gcatggcgag
     1861 aacctggctg ccgtcatggt gacctacccc tccacgcacg gggtgttcga ggagacgatc
     1921 gccgagctgt gcgcgctggt ccatgacgcg ggtgggcagg tgtacgtcga cggcgcgaac
     1981 ctcaacgccc tcgtcggcct ggcccagccg ggccggttcg gcgccgacgt ctcgcacctc
     2041 aacctgcaca agacgttctg catcccccac ggtggcggcg gccccggtgt gggtccggtc
     2101 ggggtgcgcg cgcacctcgc gccctacctg ccgaaccacc cgctgacccc ctcggccggc
     2161 ccggcaaccg gtgtgggacc ggtctcggcg gcaccgttcg ggtccgcggg catcctgccg
     2221 atctcgtggg cctacatccg gctcatgggc ggggccgggc tcaccgaggc caccaaggtg
     2281 gcgatcctca acgccaacta tgtcgccgcc cggctgcgcg agcactaccc ggtgctcttc
     2341 tccgggccgg gcgggctcgt cgcccatgag tgcatcctcg acctgcgcgc gatcacccgg
     2401 gagaccggcg tcaccgtcga cgacgtcgcc aagcggctca tcgactacgg cttccacgcg
     2461 ccgacgacgt ccttcccggt cgcgggcacg ctcatggtcg agccgaccga gagcgaggac
     2521 ctcggggagc tcgaccggtt ctgcgaggcg atgatcgcga tccgcgccga gatcgaggcc
     2581 gtgcacaacg gcgtcgtctc cgcggcggac agtgtgctcc gccacgcgcc gcacacggcc
     2641 gagtcactgg cgggggagtg ggaccacccc tacgaccgcc tggccgcggc cttccctccc
     2701 ggcgtcgaac cgaccgagaa gtactggcca ccggtccggc gggtcgacaa tgcgtatggc
     2761 gaccgtcacc tcgtgtgctc ctgcccgcct cccgaggcgt tccaggagta gccgctacgc
     2821 cgcggccgac gtgcccttga cctcgatggc cgagccggtc tcggacttgc ggaccaggac
     2881 ctgagccggg attcgcgccc gcagctcgcc gacgtggctc acgatgccga cggcccggcc
     2941 gccttcgcgg agggagtcca ggacgtccat gacctgctcg aggctgtcct cgtcgaggct
     3001 gccgaacccc tcgtcgacga agagagtctg caggtcgaag ccaccggcct gcgcccgcac
     3061 cgcatcgccg aggcccaggg ccagggccag ggaggccatg aacgcctccc cgccggagag
     3121 ggtcgacgtc tcccgggtct gcccggtcca ggcgtccttg acgacaaagc ccagaccgga
     3181 gcgagcgccg cgggcggcgc ggctgtcggt gtggccgagg gtgtagcggc cctcgcccat
     3241 gcgcacgagc cgttcgttgg ccagggccac gatctcttcg agtcggccgg cgaggacgta
     3301 ggccgacagg cgcatccgtc tgctgttgtt gacaccggtg ccggccgcga cctcggcgag
     3361 ctcggacacg acggcatact cgctctgtct cggccccaag gagtcgagca gtccttcgat
     3421 g
//
LOCUS       DATAIX010000479         1759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_175076, whole genome shotgun sequence.
ACCESSION   DATAIX010000479 DATAIX010000000
VERSION     DATAIX010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175076"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VHM65_07190"
     CDS             <1..1111
                     /locus_tag="VHM65_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2705527.1"
                     /translation="LLYFTSGTTRRPKLVEHTQVSYPVGHLSTMYWLGLLPHDVHLTI
                     SSPGWAKHAWGCFFTPWIAEATIFVYNYARFDAQALMAQMRRAGVTTFCAPPTVWRML
                     IQSDLSGGAGALREAIGAGEPLNPEVIGQVEKHWGLTIRDGFGQTETTCQIGNSPGQP
                     VTAGSMGRPLPGVPVVLVDPLTGAPSDTEGEICLELSAHPVNLMTGYLDDPEQTEQAM
                     SAGFYHTGDVATRDERGYITYIGRTDDVFKSSDYKVSPFEVESALIEHPAVAEAAVVP
                     TPDPVRLSVPKAYVMLAPDWEPTAETAYAILKHARETLPPFLRVRRIEFGELPKTISG
                     KIRRVELRQEEDRRDELGERGVKEWRDTDFPQLKG"
     gene            complement(1297..>1759)
                     /locus_tag="VHM65_07195"
     CDS             complement(1297..>1759)
                     /locus_tag="VHM65_07195"
                     /inference="COORDINATES: protein motif:HMM:NF015424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX2705528.1"
                     /translation="RVVQEGTPAEVARRPATDFVARLVGLNLYPGTLVDPVTCCVELD
                     GGGVLYAAASGRPAPRAGSRMLVAVAPNAVSVHTHRPDPGSARNLWSGTVVGLELLAE
                     RVRVAVSGAPDVVVDVTAAAVAELALEPGRQVWLTAKATEVTSYPDLDTRP"
BASE COUNT          270 a          629 c          610 g          250 t
ORIGIN      
        1 gctgctctac ttcacctccg gcacgacccg gcggcccaag ctcgtcgagc acacgcaggt
       61 gtcctacccc gtcgggcacc tgtcgacgat gtactggctc gggctgctgc cgcacgacgt
      121 ccatctgacg atcagctcgc ccggctgggc caagcacgcc tggggctgct tcttcacccc
      181 gtggatcgcc gaggcgacga tcttcgtcta caactacgcg cgcttcgacg cccaggcgct
      241 gatggcccag atgcgccggg ccggggtgac gacgttctgc gcgccgccca ccgtgtggcg
      301 catgctcatc cagtccgacc tgtccggtgg cgcgggtgcg ctgcgcgagg cgatcggtgc
      361 cggcgagccg ctcaaccccg aggtcatcgg ccaggtcgag aagcactggg gcctgacgat
      421 ccgagacggg ttcggccaga ccgagacgac gtgtcagatc ggcaactcgc ccggtcagcc
      481 ggtgacggcc gggtcgatgg gccgtcccct gcccggggtg ccggtcgtcc tggtcgaccc
      541 cttgaccggc gcgcccagcg acaccgaggg cgagatctgc ctcgagctgt cggcccaccc
      601 ggtcaacctc atgaccggct acctcgacga cccggagcag accgagcagg cgatgagcgc
      661 cggcttctac cacaccggcg acgtcgccac ccgggacgag cgcggataca tcacgtatat
      721 cgggcgcacg gacgacgtgt tcaagtcctc ggactacaag gtctcgccgt tcgaggtgga
      781 gagcgcgctc atcgagcacc cggccgtcgc cgaggccgcc gtagtgccca cgccggaccc
      841 ggtgcggctg tcggttccca aggcctacgt catgctcgcg cccgattggg aacccacggc
      901 cgagacggca tacgcgatcc tcaagcacgc ccgcgagacg ctgccaccct tcctgcgagt
      961 gcggcggatc gagttcggtg agctgcccaa gacgatctcg ggcaagatcc gccgggtcga
     1021 gctgcgccag gaggaggacc gtcgcgacga gctcggcgag cgcggggtaa aggagtggcg
     1081 cgacaccgac ttcccccagc tcaagggctg agcccgctag gcgcggacgc cccaactggc
     1141 caagcccgcg ccctgagact gattcatccc attttgaccc aatatcgccg acccgcattc
     1201 ctcgtttgac ctgtgccgct ggcacgggac gcgtgccggg ctgcaccgcc gccaccccgc
     1261 tcgttcagcc gagcgtgcgc gccatatgct cgcctcctag ggtcgagtgt ccaggtccgg
     1321 gtatgacgtg acctccgtgg ccttcgcggt gagccacacc tgacgacccg gctccagggc
     1381 cagctcggcc accgcggcgg cggtcacgtc gacgacgacg tcgggggcgc ccgagacagc
     1441 cacgcggacc cgttcggcga gcagctccag accgactacg gtgccgctcc acaggtttcg
     1501 cgcggatccg gggtcgggtc ggtgggtatg gacggagacg gcgttcggtg caacggcaac
     1561 gagcatccgg ctgccagccc ggggggccgg ccggccggac gctgcggcat acaggacgcc
     1621 gccgccgtcg agctcgacgc agcaggtgac ggggtcgacg agcgtgccgg ggtagaggtt
     1681 gaggccgacg aggcgggcga cgaagtcggt ggccgggcgc cgggcgacct cggcgggtgt
     1741 cccttcctgg acgacccgg
//
LOCUS       DATAIX010000480         5240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_86223, whole genome shotgun sequence.
ACCESSION   DATAIX010000480 DATAIX010000000
VERSION     DATAIX010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86223"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..1148)
                     /locus_tag="VHM65_07200"
     CDS             complement(216..1148)
                     /locus_tag="VHM65_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX2705529.1"
                     /translation="MPETRHLLVPEGLEGERVDAAMARLFGLSRSKAAELASAGGVLI
                     NDHAAGKSDRVKAGDQISVRLPRANGHQPVAVVAQPVPGMRIIYDDDDLVVVDKPVGV
                     AAHPSVGWTGLTVLGGLAASGYRIATSGASERQGVVSRLDVGTSGLMVVAKSETAYSR
                     LKSAFKSRTVDKNYHALVQGLPDPLVGTIDAPIARHPGHDYKFAVRERGKHAVTHYSV
                     IEAYRHASLLDIHLETGRTHQIRVHFAALHHPCCGDLTYGADPRLAARLGLQRQWLHA
                     VGLGFEHPRTGEYVSFTSDYPPDLQHALDVVSRA"
     gene            complement(1141..1632)
                     /gene="lspA"
                     /locus_tag="VHM65_07205"
     CDS             complement(1141..1632)
                     /gene="lspA"
                     /locus_tag="VHM65_07205"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX2705530.1"
                     /translation="MHQILAGVAVVTFVLDQSTKWLAIQALNGQPPVDLVGSFLRLSL
                     IRNAGAAFSLGTGATWVLTALAATIVVVLVRVSRKLGSLGWTLSLGLMLGGAVGNLTD
                     RLLRSPGPGRGHVVDFIDYNGWFIGNAADIAIVGAGGLIALLALRGVGIDGRSQAQER
                     SDA"
     gene            complement(1689..2189)
                     /locus_tag="VHM65_07210"
     CDS             complement(1689..2189)
                     /locus_tag="VHM65_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX2705531.1"
                     /translation="MTTKNAAASAASPAPESGTPDRSPRSRSAKKSVLTVTAPPVRAS
                     EKPWTAQELADMRAAIESDIRQLRAEIDTAQRDLAGLMRDAGDGSGDDQADAGAKTFE
                     REQEITLVNNARDLLEQNRHALARLDEGRYGVCESCGEPIGKFRLQAAPRATLCVTCK
                     QKQERR"
     gene            complement(2452..3438)
                     /locus_tag="VHM65_07215"
     CDS             complement(2452..3438)
                     /locus_tag="VHM65_07215"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX2705532.1"
                     /translation="MALTPELILNKHFQTTQFRKGYDERDVDDFLDEIVVEMRSLIAA
                     RDEIQEQLNDCRAGRGLTPAAPPLDGDADLAPITESIEAAERSAQERIEAANASADEA
                     ERDAQHRIDTANARSQEAEGTPQERLDAANARAEAAQQAAPTAAVGAGHTASAASVIE
                     LAQRLHDEHVAQGEATRDQLLGEGQARHDELVSTAQAQHDELVSAAQSRHDELLSAGQ
                     TKHDELVSEATARHEQLITEARERSTGMLAEAQQRKAALIEELGREKSLLEQKIEQLK
                     GFERTYRQQLKAYLESQLEELAHTGVQAPGARSAGDGDGDGHGDGDGEQSGE"
     gene            complement(3546..3827)
                     /locus_tag="VHM65_07220"
     CDS             complement(3546..3827)
                     /locus_tag="VHM65_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX2705533.1"
                     /translation="MAAVWSLVSLVLFLFFLALISRLVFDWVQVFAREWSPRGPLLVV
                     AEGIYSVTDPPLKALRKVIPSLTIGGMRIDLSFLVLIIIVSILMNLPVP"
     gene            complement(3874..4341)
                     /gene="sepF"
                     /locus_tag="VHM65_07225"
     CDS             complement(3874..4341)
                     /gene="sepF"
                     /locus_tag="VHM65_07225"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEX2705534.1"
                     /translation="MAGALRKTMVYLGFAEDDEYDRYDGNEAERTHEDRRERSAAVTH
                     LPARTPVAQVVRDSDVGPLNRITTIHPRTYNEAKTIGESFREGTPVIMNLGDMDDSDA
                     KRLVDFAAGLVFGLHGSIERVTSKVFLLSPSHVEVAAEETEAPAVSRTFFNQS"
     gene            complement(4430..5167)
                     /locus_tag="VHM65_07230"
     CDS             complement(4430..5167)
                     /locus_tag="VHM65_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX2705535.1"
                     /translation="MTRKPGPPDLARLTRRDEIAHGLSAVTERMARACAAAGRDPAEL
                     TLVVVTKLHPVSDLRLLRELGVRDIGENRDQEASDKVDAAHAAGIAGLRVHFIGQLQT
                     NKAGSVTRYADVVHSVDRPKLVRALDRGALAAGRRVGALVQVSLDERPDRGGAAPSAA
                     LDLAALVARSQSLDLLGVMAVAPHHGDPDPAFARLREVADEVRGQWPAATWVSAGMSA
                     DLEAAIAHGATHLRVGSAILGTRQPLR"
BASE COUNT          735 a         1761 c         1894 g          850 t
ORIGIN      
        1 cacgtggccg tggtcggtcg tcgccagggc cggcataccc atccgggcgg catgggtgaa
       61 cagctcgtcg atccgggccg cgccgtcgag catggagtac tcggtgtgca cgtgcagatg
      121 gacgaagctg gggtcggagg ctgaggcgag cgacatcgga gccgagtcta cgtcgccggt
      181 atgacgggtc cccgcgtgtc gagcgcgtgt tgtcctcagg cgcgggagac cacgtcgagg
      241 gcgtgctgca ggtccggcgg atagtcgctc gtgaagctga cgtactcccc cgtgcggggg
      301 tgctcgaagc cgagacccac cgcgtgcaac cactgccgtt gcaggccaag ccgggccgca
      361 aggcgagggt cggcgccata ggtgaggtcg ccgcagcagg ggtgatgcag ggccgcgaag
      421 tggacccgga tctggtgtgt ccgacccgtc tcgaggtgga tgtcgagtaa agaggcatgc
      481 cggtaggcct cgatgaccga gtagtgggtc accgcatgct tgccccgctc ccgcacggcg
      541 aacttgtagt cgtggccggg gtggcgcgcg atgggcgcgt cgatcgtccc gacgagcggg
      601 tcgggcagcc cctggacgag ggcgtggtag ttcttgtcga ccgtccgtga cttgaatgcc
      661 gacttgagcc ggctgtaggc ggtctcgctc ttggcgacga ccatgagacc ggacgtcccg
      721 acgtcgaggc ggctgacgac cccctggcgc tcggacgcgc ccgaggtggc gatccggtat
      781 cccgaggctg cgaggccacc gagcacggtc aggccggtcc agccgacgct cgggtgggcc
      841 gcgaccccca cgggcttgtc cacgacgacc aggtcgtcgt cgtcatagat gatcctcatg
      901 ccagggaccg gttgggccac gacggccacg ggctggtggc cgttggcccg gggcagcctg
      961 acgctgatct ggtccccggc cttgacccgg tcggacttgc ccgcggcatg gtcgttgatc
     1021 agcacccccc ccgcggaggc aagctccgcg gccttgctcc gggacaggcc gaagagccgg
     1081 gccatcgcgg catcgacccg ctcaccctcg agaccctcgg gcacgagcag gtgccgtgtc
     1141 tcaggcatcg gagcgctcct gtgcctgcga ccggccgtcg atcccgaccc cgcgcagggc
     1201 cagcagcgcg atgaggcctc cggcgccgac gatggcgatg tcggcggcgt tgccgatgaa
     1261 ccagccgttg tagtcgatga agtccacgac gtgcccccgg ccggggccgg gagagcgcag
     1321 tagccggtcg gtgaggttgc cgacggcgcc gccgagcatc aggccgaggg agagtgtcca
     1381 gccgaggctg cccagcttgc ggctcacccg aacgaggacc acgactatgg ttgcggccag
     1441 agccgtgagc acccaggtcg cccctgtgcc gagcgagaac gccgccccgg cgttgcggat
     1501 caggctgagg cgcaagaacg atccgacgag gtcgacgggg ggctggccgt tcagcgcctg
     1561 gatcgccagc cacttcgtgc tttggtccag gacgaaggtg acgacggcga cgcccgcgag
     1621 gatctggtgc aagcgccgtc gacgggccgg gtcgggtcgc tgcgggacgt cacatgagga
     1681 cggaccgctc agcgacgttc ctgcttctgt ttgcatgtca cgcatagcgt cgcacgcggc
     1741 gcggcctgaa ggcgaaactt gccgatgggc tcaccgcacg actcgcacac gccgtatcgt
     1801 ccctcgtcca gcctggccag cgcatgccgg ttctgttcga gcaggtcgcg ggcgttgttg
     1861 acgagggtga tctcctgctc gcgctcgaag gtcttggccc cggcatcggc ctggtcgtcg
     1921 ccggaaccgt ccccggcgtc gcgcatcagc ccggccaggt cacgctgggc cgtgtcgatc
     1981 tcagcgcgga gctggcggat gtcggactcg atcgcagccc gcatatcggc gagctcctgt
     2041 gccgtccagg gcttctcgct cgcgcggacc ggcggtgcgg tgaccgtcag gaccgacttc
     2101 ttggccgatc gcgaccgcgg gcttcggtcc ggcgtccctg attctggcgc cggagacgcg
     2161 gctgacgcgg cggcgttctt cgtggtcatg cagacccctc caagtgctcg agctgacggc
     2221 tcgcgcccga ccgatcgctc gcgcgactcg gggcacgcta tcacgtgcgt ggcggggttg
     2281 aggacaggcc aggattagcc ccggctcaac gagagcaccg gggtgttgca cacgagacac
     2341 tatcgcgaga gtgcacgagg gcccgcacaa ggaccgcgag cgagccttcg gcgtggctgt
     2401 gacaccccga gaccgcccag atcgatcgtt gccgtcgccc gtcggccgga cttactcccc
     2461 gctctgctcg ccgtcgccgt cgccatgacc gtcgccgtcg ccgtcgccgg cggagcgcgc
     2521 ccccggtgct tggacgccgg tatgtgccag ctcctcgagc tggctctcca gatacgcctt
     2581 gagctgctga cggtaggtcc gctcgaagcc cttgagctgc tcgatcttct gctcgagcag
     2641 gctcttctcg cggcccagct cctcgatcag ggcggccttc ctctgctgcg cttcggccag
     2701 catcccggtc gatcgctccc gcgcctcggt gatgagctgc tcgtggcggg ccgttgcctc
     2761 ggacacgagc tcgtcgtgct tggtctgccc ggcgctgagc agctcgtcgt gcctggactg
     2821 agcggcgctg accagctcgt cgtgctgcgc ctgcgcggtg gacacgagct cgtcgtggcg
     2881 tgcctggccc tcaccgagga gctggtcccg ggtcgcctcg ccctgggcga cgtgctcgtc
     2941 gtggagccgc tgggccagct cgatcacgct cgcggcgctg gcagtgtgtc cggcgccgac
     3001 ggctgcggtg ggtgcggcct gctgcgccgc ctcggcgcgg gcattggctg cgtcgaggcg
     3061 ctcctgcggg gtgccctccg cctcctgcga ccgcgcgttg gccgtgtcga tgcggtgctg
     3121 cgcgtcgcgc tcggcctcgt cggcgctggc gttcgctgcc tcgatgcgct cctgcgccga
     3181 tcgctcggct gcctcgatcg actcggtgat cggagcaaga tccgcgtcgc cgtcgagcgg
     3241 cggagcagcc ggcgtcaggc cgcggccggc ccggcagtcg ttgagctgct cctggatctc
     3301 gtcgcgcgcg gcgatcaacg agcgcatctc cacgacaatt tcgtccagga agtcgtcgac
     3361 gtcccgctcg tcgtagccct tgcggaactg ggtggtctgg aagtgcttgt tgaggattag
     3421 ctcgggcgtc agcgccatgt gtggtcacct cgtgggttgg gacggtgcag ggcaaccgta
     3481 gcgaatgggg cgaccagggc ccaacccggg cgcggtgacc gtccagggcg tccggcccgg
     3541 tcaggtcagg gcaccggcag gttcatgagg atcgagacga tgatgatgag gacgaggaac
     3601 gacaggtcga tcctcatgcc cccgatggtc aggctgggga tcactttgcg cagcgccttg
     3661 agcggggggt cggtcaccga gtagatgccc tcggcgacga cgagcagcgg accgcggggg
     3721 ctccactcac gggcgaagac ctgcacccag tcgaagacga gccggctaat gagcgccagg
     3781 aagaagagga acaacaccag cgagacgagg gaccacacgg cagccatggt cctagtctcg
     3841 ctgattgtct gagaaaaccg gcaagcgccg ctgtcagctc tggttgaaga acgtccgcga
     3901 cacggcgggg gcctcggtct cttcggccgc gacctcgacg tgtgagggtg agagcaggaa
     3961 caccttgctc gtgacccgct cgatcgagcc gtgcagaccg aagacgagac ctgcggcgaa
     4021 gtcgacgagg cgcttggcgt ccgagtcgtc catgtcaccg aggttcatga tgaccggtgt
     4081 cccctcgcgg aaggactcgc cgatcgtctt ggcctcgttg taggtgcggg ggtggatcgt
     4141 cgtgatccgg ttcagcgggc cgacgtcgga gtcgcgaacg acctgcgcca cgggggtacg
     4201 ggccggaagg tgggtcacgg cggcactgcg ttcacggcgg tcctcgtggg tgcgctcggc
     4261 ctcgttcccg tcgtaacggt cgtactcgtc gtcctcggcg aaaccgaggt agaccatcgt
     4321 cttgcgaagc gcccctgcca tggcggactc ccttatggtt tggccggtca gcgctgccgc
     4381 cgggtcgtgc tgcggtggtc gcccacacgg gcgggtcgtg gtgctgacgt tagcgaagtg
     4441 gctgccgcgt tccgaggatt gcgcttccga cacgcaggtg tgtcgcccca tgggcaatcg
     4501 ctgcctccag atcggcgctc atcccggccg agacccaggt cgccgccggc cactgtcccc
     4561 ggacctcgtc agcaacctcg cgcagcctgg cgaaggcggg gtccgggtcg ccgtgatgtg
     4621 gcgcgacagc catgaccccg aggaggtcga gggactgcga ccgggcgacg agggcggcca
     4681 ggtcgagggc ggcactcggc gcggcgccac cccggtccgg ccgctcgtcg aggctgacct
     4741 ggaccagtgc cccgacccgc cggccggccg ccagcgcgcc gcggtccagt gccctgacca
     4801 gcttgggccg gtcgaccgag tggacgacgt cggcgtaccg cgtgaccgag cccgccttgt
     4861 tggtctggag ctggccgatg aagtggaccc gcaggccggc gataccggcc gcgtgcgccg
     4921 cgtcgacctt gtccgaggcc tcctggtccc ggttctcccc gatgtcgcgg acccccagct
     4981 cgcgcagcag gcgaaggtcc gagaccggat gaagcttggt gacgaccacg agagtcagct
     5041 cagccgggtc gcgtccggcc gctgcacagg cccgggccat ccgctcggtg acggcgctga
     5101 gcccgtgggc gatctcgtcg cgccgcgtca accgcgcaag gtccggcgga cccggcttcc
     5161 gcgtcatgag cgaaccccct tcatctccct gatcatggcg tgcgaggctg caggacgacg
     5221 actccggcga agcgtccggt
//
LOCUS       DATAIX010000481         5293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_86232, whole genome shotgun sequence.
ACCESSION   DATAIX010000481 DATAIX010000000
VERSION     DATAIX010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86232"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="VHM65_07235"
     CDS             <1..929
                     /locus_tag="VHM65_07235"
                     /inference="COORDINATES: protein motif:HMM:NF044969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705536.1"
                     /translation="LGYFPEPIRVHYASDLAAHPLRREIVTNAVVNSMVNRGGITFAY
                     RAVEETGATVEQVSRAFVVCREVFNLAGFVRQVEALDNHVSTSTQTALYLEFRRLLDR
                     AVRWFLTNRPTSLDVAAEVDRFSATVAKLGPQIPELLQGGERKRLERRAREMEKGGVP
                     ADFALSVAALLDQFSLLDIVELATETLVPAAEVAAIYYVTSERFGIDEMLTRVTRLPR
                     DDRWDALARGALRDDLYAVLETLTHAVIEASSDGQTPAKRFDAWASANAEALRRARAA
                     LGGIERLEQPGIAALSVALRTLRAVSRGGSSG"
     gene            969..2462
                     /locus_tag="VHM65_07240"
     CDS             969..2462
                     /locus_tag="VHM65_07240"
                     /inference="COORDINATES: protein motif:HMM:NF023702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HEX2705537.1"
                     /translation="MATLTEVLKTHPRLSPEDVEWLTLLVGDWQLLADLSFADLVLWV
                     SSDPGWVVVAHVRPTTGQTLFFDDQVGHLASAGRAALLSRAEADHRIVRGRDPEWGAD
                     TPIREESIPVIRRGRVLAILTRHTNLVAMRTPSRLELTYLSTADALVRMIASGDFPHG
                     AAPTGMRRGAPRVGDGVLRLDAEGQIRYASPNAVSALHRLGHVGDLAGHSLARIVTAV
                     LRDPSPVDEGLPFVLTGRQPWRTEVCSGGATVTMRAIPLTDDGVRVGAVLLLRDVSEL
                     RRREQELLTKDATIREIHHRVKNNLQSVAALLRLQARRMPLDSGRVALAEAVRRVGTI
                     ALVHETLSQGIDETVDFDAVAERALRAVVEVVSSGTQIEAVRKGKFGRLRAEDATSLA
                     MIVSELVQNAAEHGLRGDGGRIVVAAKRSTDPGGDALLTVTITDNGVGLPKGVSAPSG
                     GLGAQIVRSLVRDLQGTIEWTRARPRGTRVRFVARLRPLRRDPGAGA"
     gene            complement(2554..2802)
                     /locus_tag="VHM65_07245"
     CDS             complement(2554..2802)
                     /locus_tag="VHM65_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019483052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX2705538.1"
                     /translation="MDWRDRAACLDADPELFFPIGNTGPAIAQIEEAKIVCRSCEVIN
                     ICLKWAIDTGQDAGVWGGLSEDERRALKRRNARARRAG"
     gene            3134..3529
                     /locus_tag="VHM65_07250"
     CDS             3134..3529
                     /locus_tag="VHM65_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705539.1"
                     /translation="MERAHDPGAPRWARALAAGGSLVQAGALGALIGLYLLQMAAGAD
                     TDRGRAVMSVVLFVVLAAGLVVLGVAAWRSSGWPRTPILVWNALLVPVAISLGQSRQL
                     LGALALGIVVLVTVVGTLSLPSASEVTGG"
     gene            complement(3516..4115)
                     /locus_tag="VHM65_07255"
     CDS             complement(3516..4115)
                     /locus_tag="VHM65_07255"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX2705540.1"
                     /translation="MSPVFAHGQLRLYLLALLDEGPRHGYEVIQELERRFEGLYSPSA
                     GTVYPRLAKLEEEGLVERTDEGRKATYRITDSGRREVHERHTDLEDLRSGLDMSVRAL
                     AEDVRTRVQGQARDLRAELAAAAREARATATPLGGPEWKRDRPGREWARDLEDLVTEV
                     RDLTRRAWRSQRLTPEQQHEIRDILADTANRVLAVVNRR"
     gene            complement(4112..5059)
                     /locus_tag="VHM65_07260"
     CDS             complement(4112..5059)
                     /locus_tag="VHM65_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX2705541.1"
                     /translation="MTNQWSIEGPKVLDIGDEGETVRALKVSVVGGRVDVVTHDDSPT
                     ARVEVTSIEGPPLKVMWNGDTLRISHGEVFDQESLASLRSGHGGFTEGTFAKLRQAFD
                     FERAKVTVSISVPVGARAKLNCVSAAGLLSGLRGGVKVNTVSGSLALTELSGATDLNS
                     VSGNVECVDLDGDLKINTVSGAITVQASRVPVAKITTVSGDIALDLTNNTASITSNSV
                     SGDVTVRVPQGGYAVEANTFSGQVVANGRTLGNGQRGPVGSTGARHTEGDGALRVQAN
                     SMSGNIVILTTAVVPQDAPQDAPPADAPADSPWQQDEPR"
BASE COUNT          684 a         1845 c         1892 g          872 t
ORIGIN      
        1 tgctcggcta ttttcccgag ccgatcaggg tgcactatgc gagcgacctc gccgcgcacc
       61 cgctgcggcg cgagatcgtg accaacgctg tcgtcaactc gatggtcaac cggggtggca
      121 tcaccttcgc ctaccgcgcc gtcgaggaga ctggggccac ggtggagcag gtgtcccggg
      181 cgttcgtcgt ctgccgagag gtcttcaacc tggccgggtt cgtccgccag gtcgaagcac
      241 tggacaacca cgtctcaacg agcacccaga cggcgctgta tctcgagttc cggcggctcc
      301 tggatcgtgc ggtgcggtgg ttcctcacca accgtccgac gagcctcgat gtcgccgccg
      361 aggtcgaccg cttctccgcg acggtggcca agctcgggcc gcagatcccg gagctgcttc
      421 agggcggtga gcgcaagcga ctggaacggc gcgctcgcga gatggagaag ggcggggtgc
      481 cagccgactt cgcgctgagt gtcgccgcgt tgctcgacca gttcagcctg ctcgacatcg
      541 tcgagctggc gaccgagacc ctcgtgccgg cggccgaggt ggccgccatc tactacgtga
      601 cgtcggaacg gttcggcatc gacgagatgc tgacccgggt gacccggctc ccgcgcgacg
      661 accggtggga tgccttggcg cgaggtgccc tgcgcgacga cctctacgcg gtactcgaga
      721 cgctgaccca cgcggtcatt gaggccagct cggacggtca gaccccggcc aagcgcttcg
      781 acgcctgggc gagcgccaac gccgaggcgc tgcgccgtgc gcgggccgca ctgggcggca
      841 tcgagcgact cgagcagccc gggatcgcgg cgttgtccgt ggccctgcgg acgctgcgtg
      901 cggtgagccg cggcggctcc tcgggataac ggtcggccca cctgccgacg cggctacgct
      961 gtcggatcgt ggccaccctg accgaggtgc tgaagacgca cccccgcctc tcgccggagg
     1021 acgtcgagtg gctcacgctg ctcgtggggg actggcagct gctcgccgat ctgtccttcg
     1081 ccgacctcgt gctctgggtc agctccgatc caggctgggt tgtcgtggcc catgtacggc
     1141 ccacgacggg ccagaccctc ttcttcgacg accaggtcgg gcatctggcc agcgccggac
     1201 gggccgctct gctcagccgg gccgaggccg accatcggat cgtgcgtggc cgggacccgg
     1261 agtggggcgc cgacacgccg atccgtgagg agtcgatccc ggtcatccgc cgtggccgcg
     1321 tcctggcgat cctgacccgc cataccaacc tggtcgcgat gcgcaccccg agccgtctcg
     1381 agctgacgta cctgtcgacg gccgacgcac tcgtccggat gatcgcctcc ggggacttcc
     1441 cgcatggggc ggcacccacc gggatgcgtc gcggcgcccc tcgggtcggt gatggcgtgc
     1501 tgcgcctcga cgccgagggc cagatcaggt atgccagccc caacgcggtt tcggctctgc
     1561 atcggctcgg ccatgtcggt gacctggcgg ggcactcgct ggcccgaatc gtcacggccg
     1621 tgctccgaga cccctcgccg gtcgacgagg gcctgccctt cgtcctcacc ggccgtcagc
     1681 cctggcgcac cgaggtctgc tcgggcggcg cgacggtgac gatgcgcgcc ataccgctca
     1741 ccgacgatgg cgtccgggtc ggcgccgtcc tgttgttgcg cgatgtcagc gagctgcgcc
     1801 ggcgggagca ggagctgctc accaaggacg cgacgatccg ggagatccac catcgggtca
     1861 agaacaacct tcagtcggtc gccgccctgc tgcgcctgca agcccggcgg atgccgctgg
     1921 attcgggccg ggtcgccctg gccgaggcgg tccgccgcgt cggcaccatc gccctcgtcc
     1981 acgagactct gagccagggc atcgacgaga cggtcgactt cgacgcggtc gcggaacgcg
     2041 ccctcagggc cgtcgtcgaa gtcgtctcga gcggcacgca gatcgaggca gtccgcaagg
     2101 gcaagttcgg gcggttgcga gccgaagacg cgacgtcact ggcgatgatc gtcagtgagc
     2161 tggtccagaa cgcggccgag cacggactgc gcggggatgg cggacgcatc gtggtcgctg
     2221 ccaaacgcag caccgatccc ggaggcgacg cattgttgac ggtgacgatc accgacaacg
     2281 gcgtgggcct gcccaagggg gtgtcagcgc cgtcaggagg gctcggcgcc cagatcgtgc
     2341 ggtctctcgt ccgcgatctc cagggcacga tcgagtggac gcgagcccgg ccgcgcggga
     2401 cccgggtccg gttcgtggcc cggctgcggc cgctgcgccg cgaccccgga gcgggcgcat
     2461 agctggtgct cggcgggtgg ccgatcgggg ctcagcccgc tcggcgggtg gccgatcggg
     2521 gctcagcccg cccggcgggt ggccgatcgg ggctcagccc gcccggcggg cccgggcgtt
     2581 gcgccgcttg agcgcgcgcc gctcgtcctc ggacaggccg ccccacacgc cggcatcctg
     2641 gcccgtgtcg atggcccact tgaggcagat gttgatgacc tcgcagctgc ggcagacgat
     2701 cttggcctct tcgatctggg cgatcgccgg accagtgttg ccgatcggga agaacagttc
     2761 cgggtcggcg tccaggcagg cagcgcggtc gcgccagtcc atgaggatcg tgctccttga
     2821 tgatgggttt cgtgcgtgtg ggctcggagt ggggtaccgt tcacgctcag gttggcggca
     2881 ccactgctgg cgtgccgtgg tgacagccct gccggctcgg tgaagagcct ccctgacaac
     2941 gtcggacaac gctgtcctgt tccgaggcca gggtccagtc tggtcccact tgtggccgcg
     3001 ggcaaccctt gtgacctgat tctcaaggtg tgtcgccccc gtttctggcc gtgcacgccg
     3061 ccgcggaccc acccgttcgc ccgggcccgg gcccgggctc gggcccgggc tcgggcccga
     3121 taggctcgcc accatggagc gggcgcatga ccccggtgca ccgcgatggg ctcgggccct
     3181 ggcggccggt gggtccctcg tgcaggctgg tgccctgggt gccttgatcg gcctctacct
     3241 ccttcagatg gctgccgggg cggacacgga ccgcgggcga gcggtgatgt cggtcgtcct
     3301 cttcgtggtg ctcgccgccg ggctggtcgt gctgggcgtg gccgcgtggc gctcatcggg
     3361 gtggccgcgc accccgatcc tggtctggaa cgccctgctg gtcccggtcg ccatctcgct
     3421 cggccagtcc aggcagctgc tcggcgccct ggcgctcggc atcgtcgtcc tggtcacggt
     3481 cgtgggcacc ctgtccctgc cctccgcgtc cgaggtcacc ggcggttgac gacggcgagc
     3541 acccggttgg cggtgtcggc gaggatgtcg cggatctcgt gctgctgctc aggggtgagc
     3601 cgctgcgacc gccacgcacg ccgggtgagg tcccggacct cggtgacgag gtcctcgaga
     3661 tcccgggccc actcgcgccc cggacggtcc cgcttccact cgggcccacc gaggggggtc
     3721 gcggtcgccc gggcttcgcg tgccgccgcc gccagctcgg cgcgcaggtc ccgcgcctgg
     3781 ccctggaccc gggtgcgaac gtcctcggcg agtgcgcgca ccgacatgtc gagcccgctg
     3841 cgcaggtcct ccagatcggt gtggcgctcg tgcacctcgc gccggcccga gtcggtgatg
     3901 cggtaggtcg ccttgcgtcc ctcgtccgta cgctcgacca gaccctcctc ctcaagcttg
     3961 gccagccggg ggtagaccgt gccggcgctg ggtgagtaga ggccctcgaa gcggcgttcc
     4021 agctcctgga tcacctcgta cccgtggcgc ggtccctcgt cgaggagcgc gaggaggtag
     4081 aggcgcagct gtccgtgggc gaagaccggg ctcatcgagg ctcgtcctgc tgccacggcg
     4141 agtccgcggg ggcatcggcg ggcggcgcgt cctgaggggc atcttgcggc accacggctg
     4201 tggtgaggat gacgatgttg cccgacatcg agttggcctg gacccgcagg gctccgtcgc
     4261 cctcggtatg gcgcgccccg gtgctcccga ccgggccccg ctggccgttg cccagcgtgc
     4321 ggccgttggc gacgacctgt ccgctgaacg tgttggcctc gacggcatac ccgccctggg
     4381 ggacacgcac ggtgacatcg ccagagaccg agttcgacgt gatcgaggca gtgttgttgg
     4441 tgaggtcgag cgcgatgtca ccggagaccg tggtgatctt ggcgaccggc acgcggctgg
     4501 cctggaccgt gatggccccg gagacggtgt tgatcttgag atctccgtcg aggtcgacgc
     4561 attcgacgtt gccgctgacg ctgttgaggt cggtcgctcc cgacagctcg gtcaaggcga
     4621 gcgagccgga gaccgtgttg accttgaccc cgccccgcag cccgctcagg agcccggcgg
     4681 cactgacgca gttgagcttg gcccgggcgc cgaccggcac cgagatgctc accgtgacct
     4741 tggcccgctc gaagtcgaag gcctggcgca gcttggcgaa ggtgccttcg gtgaagccgc
     4801 cgtgaccgga ccgcaacgag gccagggact cctggtcgaa gacctcgccg tgactgatcc
     4861 tgagcgtgtc gccgttccac atcaccttca ggggcggtcc ctcgatgctc gtcacctcga
     4921 cccgtgccgt gggggagtcg tcatgggtga cgacgtcgac ccggccaccg acgaccgaca
     4981 ccttgagcgc ccggaccgtc tcgccctcgt caccgatgtc gaggaccttg ggcccttcga
     5041 tgctccactg gttcgtcatc cgtcctcctt gcctgggtcc agccgactgc tggggacacg
     5101 atatatcgcg ttccgttcac gatatatcgt gtctgcgccc agggcaaggc cgagcgaccc
     5161 cccggtcctc ggactgcgtc gtcggccgtg ccctgttgag ggctgcgttg ctgccccgct
     5221 ctcggtgtcc gctgcggatg ggtcgtgatg agacgggcga agtgactcgg cgcttccggc
     5281 ggtcacgcgt gca
//
LOCUS       DATAIX010000482         4066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_220027, whole genome shotgun sequence.
ACCESSION   DATAIX010000482 DATAIX010000000
VERSION     DATAIX010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220027"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VHM65_07265"
     CDS             complement(<1..439)
                     /locus_tag="VHM65_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705542.1"
                     /translation="MATRPSSPSARSGAPARGGSTRAGSTRATATKARDNRGGRATAR
                     PAAGRTKTKGGGLPVPLRALRGTWMGVSHVAGGAVRRVGHSARDLQPEHRRDGIGFLL
                     LALAILVAAREWWGVPGAAGNVVHAVTAGTFGRIAYALPIVLLL"
     regulatory      606..714
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00634"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class IV; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00634"
     gene            751..2082
                     /locus_tag="VHM65_07270"
     CDS             751..2082
                     /locus_tag="VHM65_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX2705543.1"
                     /translation="MSVAPCSTSLVPRTVGSGAIVPTRGGLATYANLDHGASTPALTA
                     VRDAVDATVATYSSVHRGNGYHSRVTSAWYEQARAEVAAFVGARPDDTVIFTRNTTDS
                     VNLLARTLPAGTTVVVFASEHHSTLLAWPQATVRLPVPRSVGAALASLEAVLASDTSA
                     HRLVVVAGASNVTGEIWPITEIVGLAHRYGARVALDAAQYAPHRRVDLAATGLDYVAF
                     SGHKIYAPYGAGVLAGRRDWLDAGAPYLRGGGATARVTSDEVTWATGASRHEGGSPNV
                     VGAVALAAACATLTRHWDAVETHEAELGARLRQGLALIPGVRTYSLFGAGHERAAVSA
                     FTVDGVDSSLVSAVLSADHGIGVRDGKFCAHLLVDVLLGSDLGLAHRAAGHRTAVRAS
                     VGLATTAEHVERLLVAVAELADGRAPGGYVADAGGYVVADDPRELVPAAPW"
     gene            2303..2959
                     /locus_tag="VHM65_07275"
     CDS             2303..2959
                     /locus_tag="VHM65_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705544.1"
                     /translation="MFVLTIDQRRSRAGRDLVPEALALATAGAGTALGRPFERTVGDE
                     LQGVLTEAGPTVAVVLALAQRSQWHIGIGVGAVEEPLPSSTRAARGPAFQAARDAVEE
                     ARSSSRHIAVRAAGYAESQFAEDHARWAETGLWLVAHVMSARSDRGWEVVNLLAQGLN
                     QREASGQLGVTQAAVSQRVSRAGWAEQTRGAELVVYHLAEAERLARLTGPPRAEQATW
                     "
     gene            2965..3342
                     /locus_tag="VHM65_07280"
     CDS             2965..3342
                     /locus_tag="VHM65_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705545.1"
                     /translation="MLPLLVAVGFGAVAVLLGSPVTMWLFRRVDRSEGTGAAHTVTGA
                     AAVLRGGMWIGYLERGATFASILLGWPEGIALAIAIKGLGRYEELRSSTTGAAERFII
                     GTFASLLFACLWAVAAREVIALV"
     gene            complement(3361..>4066)
                     /locus_tag="VHM65_07285"
     CDS             complement(3361..>4066)
                     /locus_tag="VHM65_07285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX2705546.1"
                     /translation="IEIGPGDTVLLASSLIPGNENAVYRVINGLMRLGANVVHKGNAK
                     VHVSGHASAGELLYVYNIVKPRNVIPVHGEWRHMVANADLALSTGIAPENVVLAEDGV
                     VVDLVEGQARIVGAVPCGYIYVDGSSVGETTEALLKDRRILRDEGFITCIVVIDAASG
                     KIAAGPEITARGFAEDEAVFEAVIPKVVAALEEATRGGITDAYQLQQIIRRTIGTWVG
                     GKIRRRPMIIPVVIEA"
BASE COUNT          542 a         1482 c         1449 g          593 t
ORIGIN      
        1 agaggagcag gacgatcggc agggcgtagg cgatacgccc gaaggtgccg gcggtcacgg
       61 catggacgac gttgccggcc gctccgggga cgccccacca ctcgcgggcc gcgacgagga
      121 tggcgagcgc gaggagcagg aagccgatcc cgtcgcggcg gtgctcgggc tgcagatccc
      181 gggccgagtg gccgacccgg cggacggctc ccccggcaac gtgggagaca cccatccagg
      241 tcccccgcag ggcgcgcagc ggcacgggga gcccgcctcc cttggtcttc gtgcggccgg
      301 cggccggccg ggccgtagcc cggccacccc ggttgtcgcg cgccttggtc gctgtggctc
      361 tggtcgagcc ggcccgggtc gagcccccac gggcaggggc ccccgaacgc gcggacggtg
      421 aggacggacg tgtcgccatg gtcgcagagt aggcgatcga ccccttcgac ccctgcgtca
      481 acacgcgtca cacccgtaac gcgagggtgg tgagacccgc gtacccccct gggtgtgacc
      541 acatcgcgag agagacaggt cgccatgtgg acgtctcacg cacgcccggt ctaccctcgt
      601 acccaggtca cgagtgccag cgcccagccc cggcttgctg gccagcaacc ctcctccgcg
      661 gcggggtgct ccgggtgacg accaggccgt cgatgccgac ggcaagcgcg gactcgcacc
      721 cggcgggtcc catgacccca ggagcatccc gtgtccgtgg ccccctgctc gacctcgctc
      781 gtcccccgca cggtcggatc cggcgcgatc gtcccgaccc ggggcggcct ggcgacctac
      841 gccaacctcg accacggtgc ctcgactccc gcgctcaccg cggtccggga cgccgtcgat
      901 gcgacggtgg cgacctactc gtcggtccat cgcggcaacg gctaccactc gcgggtgacg
      961 agcgcatggt acgagcaggc ccgggccgag gttgccgcgt tcgtcggggc acgacccgat
     1021 gacacggtga tcttcacccg caacacgacc gactcggtca acctgctcgc gcgcaccctg
     1081 cccgccggca cgaccgtcgt cgtcttcgcg agcgagcacc actcgacgct gctcgcctgg
     1141 ccgcaggcca cggtgcgcct gccggtcccg cgcagcgtcg gcgccgcgct cgcgtcgctc
     1201 gaagcggtcc ttgcgtcgga cacgtcggcc caccggctcg tcgtcgttgc tggggcctcg
     1261 aacgtcaccg gcgagatctg gcccatcacc gagatcgtcg ggctcgcgca ccggtatggc
     1321 gcgcgggtgg ctctcgacgc cgctcagtac gctcctcacc gccgagtcga cctcgccgca
     1381 acgggtctcg actatgtcgc cttctccggc cacaagatct atgccccgta tggcgcgggg
     1441 gtcctggccg ggcgccggga ctggctcgac gcgggcgcgc catacctgcg tggaggtggg
     1501 gccacggcgc gcgtgacgag cgacgaggtc acctgggcca ccggggcctc ccgacacgaa
     1561 ggcgggagcc ccaacgtcgt cggtgcggtg gcgctcgcgg cggcctgcgc gacgctgacc
     1621 cggcactggg acgccgtcga gacgcacgag gctgagctcg gggcgcggct gcgtcagggc
     1681 ctggcgctga tccccggcgt gcggacctac tcgctcttcg gggccgggca tgagcgagcg
     1741 gcggtgtcgg cgttcaccgt cgacggcgtg gactcctcgc tcgtgtcggc cgtgctctcg
     1801 gccgaccacg ggatcggggt gcgcgacggc aagttctgcg cgcatctgct cgtcgacgtg
     1861 ctcctgggca gtgacctggg cttggcgcac cgagccgcgg ggcaccggac ggccgtgcgc
     1921 gcgagcgtcg ggctggcaac caccgccgag cacgtcgagc gcctgctcgt cgcggtcgcc
     1981 gagctcgccg acgggcgggc gccaggcggg tatgtcgccg atgcgggtgg ctacgtcgtg
     2041 gccgacgacc cccgtgagct cgtcccggcc gcaccgtggt gaccctcgtg gggggccgcc
     2101 gcgccgcgcg ccatcacgca cgcacgcacg tatgccgtgc ccgccatcag cgggctgcgc
     2161 atagcctctt gcggcgacac ccccagcttg gcgctcggct cgcgcctgcc gcgacacctc
     2221 gaccgccttc aggacgtaag gtctcagact taactttcac gaattaaggg tgtagcctta
     2281 tcactaagga aggaggtgcc ccatgttcgt cctcaccatc gaccagcgcc gcagccgcgc
     2341 ggggcgcgac ctcgttccgg aggcgctcgc gctcgccacg gccggcgctg gcaccgccct
     2401 ggggcgcccg ttcgagcgaa ccgtcggcga cgagctccag ggcgtgctca ccgaagcggg
     2461 cccgacggtg gctgttgtcc tcgccctcgc ccagcgttcc cagtggcata tcgggatcgg
     2521 cgtcggcgcc gtggaggagc cgttgccctc ctcgaccaga gcggcccggg gcccggcctt
     2581 ccaggcggcc cgcgacgccg tcgaggaggc ccgctcgagc agccggcaca tcgctgtcag
     2641 agccgccgga tacgctgagt cccagttcgc cgaggaccac gcgcggtggg ccgagacggg
     2701 cttgtggctc gtcgcacacg tcatgtcggc gcgctcggat cggggctggg aggtggtcaa
     2761 cctgctcgct caagggctga accagcgcga ggcgtccgga caactcgggg tgacccaggc
     2821 ggcggtgagc cagcgggtga gccgggccgg ctgggccgag cagacccgag gggccgagct
     2881 cgtcgtctat catctcgccg aggccgagcg tctcgccagg ctcacgggcc cgcctcgagc
     2941 ggagcaggcg acctggtgag ggatgtgctt cccctcctcg tggccgtggg cttcggcgcg
     3001 gtcgccgtcc tgctcggcag cccggtcacg atgtggctgt tccgccgggt cgaccgttcc
     3061 gagggcacag gtgcggccca caccgtgacg ggcgccgctg cggtgctccg cggcggcatg
     3121 tggatcggct acctggagcg gggggcgacc ttcgcctcga tcctgctcgg ctggcccgag
     3181 gggattgccc tggccatcgc catcaaggga ctcggccgct acgaggagct gcgcagctcg
     3241 acgaccggtg ccgcggagcg cttcatcatc ggcaccttcg ccagcctgct cttcgcctgc
     3301 ctgtgggccg tcgccgcccg cgaggtcatc gccctcgtct gaccgccggg tcgcctggga
     3361 tcaggcttcg atgacgaccg ggatgatcat cgggcgccgg cggatcttgc cacccaccca
     3421 ggtaccgatc gtgcggcgga tgatctgctg cagctggtac gcgtcagtga tcccgccgcg
     3481 cgtcgcctcc tcgagagccg ccacgacctt ggggatgacg gcctcgaaga cggcctcgtc
     3541 ctcggcgaaa ccgcgggccg tgatctcggg cccggcagcg atcttgcccg aggcggcatc
     3601 gatgacgacg atgcacgtga tgaacccttc gtcgcgcagg atccggcggt ccttgagcag
     3661 cgcttcggtg gtctccccca ccgacgagcc gtcgacgtag atgtagccgc acgggaccgc
     3721 gccgacaatc cgggcctgcc cctcgacgag gtcgacgacg acgccgtctt cggcgagcac
     3781 gacattctcc ggggcgatcc cggtggacag ggccaggtcc gcgttggcga ccatatgccg
     3841 ccactcgccg tggaccggga tgacgttgcg cggcttgacg atgttgtaga cgtacagcag
     3901 ctcgccggcg ctcgcgtgac cggagacgtg caccttggca ttgcccttgt gcacgacgtt
     3961 cgcgccgaga cgcatgaggc cgttgatgac ccggtagacg gcgttctcgt tgccggggat
     4021 gagcgaggac gccagcagca ccgtgtcccc agggccgatc tcgatc
//
LOCUS       DATAIX010000483         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_175422, whole genome shotgun sequence.
ACCESSION   DATAIX010000483 DATAIX010000000
VERSION     DATAIX010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175422"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(365..1510)
                     /locus_tag="VHM65_07290"
     CDS             complement(365..1510)
                     /locus_tag="VHM65_07290"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX2705547.1"
                     /translation="MDFGTLAVICAVALLGPVLSLPRWLHLPVVIGELVVGVVLGATG
                     LQVLHAENATFAFLAEIGFALVMFVAGSHVPIRDRALRADFTVGAGRAVAIGVLAVGV
                     GVLVARVFGTGHSALYAVLLASSSAGLVLPIIAGNGLSGPAVVALLPQIAMADAACIV
                     ALPLAVDPPHALRALLGSVVVLAAAGVVFLALRHLEASGRRRAVHHLSEERGLAIELR
                     LSLTLLFGLAALAVLTHVSVMLAGFSLGLAVAGVGEPRRLTKQLFALTEGFFAPVFFV
                     WLGASLNLRDLVTHPSSIALGLVLGVGAVLVHGLLSLSHQPVALAVMSAGQLGVPVAA
                     ATLGAQLGLLGPGEPAALLLGALVTIAAVAVASGRAVATPAPQPSSG"
BASE COUNT          302 a          579 c          563 g          137 t
ORIGIN      
        1 gggcagatct tgcgcggcgc cataccggcc ggccctgacc ggccggaacc acctcagtga
       61 gccacccccg gcctaccgag gccgatctga ccggcccaga ccggccggaa ccacctcagt
      121 gagccacccc ggcctaccga ggtggatccg gccggtgccg accggtcaag cgatgaaatc
      181 tcacctgagc cccttggggc cgcgcggcct cgacgaccac cgacccacca ggagctcagc
      241 cgctcaccac gggcaaacag caccccgcca gcaccagagc tcccggacgc gctccgaggt
      301 caaggtcgtc ccgatccggc acaccctgcg gccgcggccg cctggggcac cagccagcga
      361 tgactcatcc tgacgacggc tgcggagccg gagtggccac ggctcgacca gaggccacgg
      421 caaccgcggc aatcgtgacg agggcgccga gcaggagcgc cgcaggctcg cccgggccaa
      481 gtagcccgag ctgagcgccg agggtggccg cggccaccgg aaccccgagc tgcccagcgc
      541 tcatcacggc gagcgcgacc ggctggtggc tcaggctgag caggccatgg acgagcaccg
      601 caccgacacc gaggacgagc ccgagcgcga tcgaggacgg atgagtcacg aggtcgcgca
      661 ggttgaggga cgcccccagc cacacgaaga agaccggcgc gaagaacccc tcggtcagcg
      721 cgaacagctg cttggtgagc cggcgcggct caccgacccc ggcgacggcc agacccaggc
      781 tgaagccggc cagcatgacc gagacgtgcg tgaggacggc cagcgccgcg agcccgaaga
      841 gaagcgtcag cgacaggcgc agctcgatgg cgagcccacg ctcctcggac agatggtgca
      901 cggccctgcg ccgacccgac gcctcgaggt gacgcagggc gaggaagacc accccggcgg
      961 ccgccaggac gaccacgctc ccgagcagcg cccgcagggc atggggcggg tcgacggcca
     1021 acggcagcgc gacgatgcag gccgcgtcgg ccatcgcgat ctgagggagc agcgccacca
     1081 cggccggccc gctgaggccg ttcccggcga tgatcggcag gacgaggccg gccgaggagg
     1141 aggcgagcag cacggcatac agcgcgctgt gacccgtccc gaagaccctg gcgaccagga
     1201 ctccgacgcc gaccgcgagg acgccgatcg ccacggcccg gccggccccg accgtgaagt
     1261 cggcccgcag cgcacggtcg cggatcggga cgtgggaccc ggcgacgaac atcacgagag
     1321 cgaagccgat ctcggccagg aaggcgaagg tggcgttctc ggcatgcagc acctgcaggc
     1381 cggtcgcgcc gaggacgaca ccgacgacga gctcgccgat gacgacgggc aggtgaagcc
     1441 agcgcgggag cgacagcacc ggcccgagca gggcgacggc gcagatgact gccagagtgc
     1501 cgaagtccat gggaccgcct cacgactggt gggatgagcc gtctgggccc actgtacgag
     1561 tgaactcacg tgccccgcgg t
//
LOCUS       DATAIX010000484         1667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_86256, whole genome shotgun sequence.
ACCESSION   DATAIX010000484 DATAIX010000000
VERSION     DATAIX010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86256"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1667
                     /locus_tag="VHM65_07295"
     CDS             <1..>1667
                     /locus_tag="VHM65_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705548.1"
                     /translation="FCMTRPKPREEDLPTYGPAFDSLCESARLFWTHQSPPRTSADLV
                     VVDLMMQDIRLALRNLTQAAALSRVLQSRIVAIVGPDPEWAREIWQYYDAERLAQLAR
                     AYGADEVLDLAALADRCADGSLRELVVAGQRLPLAEHPGADPDRFREIVDATAVRILR
                     VPRVTEPVLVDPRYGAIAARSRQFGRIYEALMGSGVAAFVTSHADYSQWGLGVDAAIR
                     HQVPVVHVQATGDLKSYALFPEDVSGDGTFRTQLTRLIGQHFDTHLWPRRDLLHDACE
                     RTAWRAKGNLGRPSWWRGGGAVSKLDIRTPAERTDIRRYAASRLGIDPGKPVVGVFNH
                     AVSDAVSCNIEIFADLADWLTETVQYAAGHPEATWLFLDHPKQANYDGTEHFEHLAER
                     FRGIPHLLFRPSMTLSKNAMWSLVDLAVTVRGSVSNEFPAYGIPAIQAGWSEWSHCGF
                     SMRADSVPQYWHLLASVLGRLCNGEQIISPDQVRRARLWLWAYRCGTDVVSPLVPFWE
                     TGQGEALYESVALAMNHIEADGDPVLVSTARMWSRREPLLTRVDLTVP"
BASE COUNT          256 a          610 c          536 g          265 t
ORIGIN      
        1 ttctgcatga ctcgacccaa accccgcgaa gaggacctgc cgacgtacgg ccccgcattc
       61 gactcactct gtgagtcggc ccggctcttc tggacccacc aatcccctcc gcggacctcc
      121 gcggacctcg tcgttgtcga cctgatgatg caggacatcc ggctggccct gcgcaacctg
      181 acccaggcgg ccgcgctgtc gcgggtcctg cagtcgcgga tcgtcgcaat cgtcgggccc
      241 gatcccgagt gggcccgtga gatctggcag tactacgacg cagaacggct cgcccagctg
      301 gcccgcgcgt atggcgccga cgaggtgctc gacctcgccg cactcgcaga ccggtgcgcc
      361 gacgggtcat tgcgggagct ggtcgtcgcg ggacagcgcc tgccactggc cgagcacccc
      421 ggcgcggatc cggaccgctt ccgcgagatc gtcgacgcga cagcggtccg gatcctgcgc
      481 gtgccccggg tcaccgagcc cgtcctggtt gacccccggt atggcgcgat cgcggcccgg
      541 agcaggcagt ttggccggat ctacgaggcc ctcatgggga gcggtgtcgc ggccttcgtc
      601 acgagccatg ccgactacag ccagtggggg ctcggagtcg atgcggccat ccgccatcag
      661 gtgccggtcg tccacgtcca ggccaccggg gatctcaagt cctatgccct cttcccggaa
      721 gacgtcagcg gtgacggcac cttccgcacg cagctcaccc ggctcatcgg gcagcacttc
      781 gacacccatc tgtggcctcg gcgggacctg ctccacgacg cctgcgagcg caccgcgtgg
      841 cgggccaagg gcaacctcgg tcggccctcg tggtggcgtg gcggcggagc cgtatccaag
      901 ctcgatatcc gcaccccggc tgagcggacc gacatacgca ggtatgccgc atcgcgcctc
      961 ggcatcgatc cgggcaagcc cgtcgtcggg gtcttcaacc acgcggtgtc tgacgcagtc
     1021 tcgtgcaaca tcgagatctt cgccgacctg gcggactggc tcaccgagac agttcagtac
     1081 gcagccggac atcccgaggc cacgtggctg ttcctcgacc accccaaaca ggccaattac
     1141 gatggaaccg agcacttcga gcacctcgcg gagcgcttcc gggggatccc tcacctgctc
     1201 ttccggccga gcatgacgct gagcaagaac gcgatgtgga gcctggtcga ccttgccgtc
     1261 accgtccggg gcagtgtcag caacgagttc cccgcgtatg gcatccccgc gatccaggcc
     1321 ggctggtccg agtggagcca ttgcgggttc agcatgcggg ccgactccgt gccgcagtac
     1381 tggcacctgc tcgcctcggt cctgggccgg ctctgcaacg gcgaacagat catctccccc
     1441 gaccaggtgc gccgggcccg cctctggctg tgggcctacc gctgcgggac cgacgtcgtc
     1501 agcccgctcg tgcccttctg ggaaaccgga cagggcgagg cgctctacga gagcgtcgcc
     1561 cttgccatga accacatcga ggcagacggc gacccggtgc tggtctcgac cgcccggatg
     1621 tggtcgcgcc gcgagccact gctcacgcgg gtcgacctca ccgtgcc
//
LOCUS       DATAIX010000485         3039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_115741, whole genome shotgun sequence.
ACCESSION   DATAIX010000485 DATAIX010000000
VERSION     DATAIX010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115741"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1498
                     /locus_tag="VHM65_07300"
     CDS             134..1498
                     /locus_tag="VHM65_07300"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HEX2705549.1"
                     /translation="MSTRPDPVTLRGERDAAVPADPGVHPLVVRTVEITDPGPLLSLL
                     PDLVPDLLPDLGPAGEAPGLAAWVRRGEGLVGWGQAASFTASGPDRFAAADAWWTALC
                     SRAVVRDEVGLGGSGPVMFGSFPFAAGSAEPGRVVVPEVVIGHRGARWWVTTASLGAG
                     VPSIGAVPGPIVAAPPRSPVGVTFADGALSPTEWEGAVGEAVVRITSGALDKVVLARD
                     LQALATEALDPRWLLTRLAERYSTTWVFTVAGLLGATPEMLVRRDKGLVTSRVLAGTI
                     RRTGDDAKDLALAGSLARSSKDLEEHEYAVRSVADALAPHCSSINVPESPFVLHLANV
                     MHLATDVAGVAAGSASALELAAALHPSAAVCGTPTEVAAGLIHELEHMDRGRYSGPVG
                     WMDASGDGEFGIALRCGQIDGRDPRAIRLYAGCGIVAGSDPEAELAESDVKLVPMRDA
                     LTSA"
     gene            complement(1528..1929)
                     /locus_tag="VHM65_07305"
     CDS             complement(1528..1929)
                     /locus_tag="VHM65_07305"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX2705550.1"
                     /translation="MTVVVDSAVVVGALVDGGATGSWAASVLSKHDLVAPHLMPVEVA
                     NILRRSQAAGLITADSATLAHMDLLDLPVSLVPYLPFAGRAWALRANLTSYDAWYVAL
                     AEALGCPLATLDRRLAGATGPLCPFLVPSLG"
     gene            complement(1926..2162)
                     /locus_tag="VHM65_07310"
     CDS             complement(1926..2162)
                     /locus_tag="VHM65_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705551.1"
                     /translation="MSKSITVRDVPDDVVRELAARAAGSGRSLQEYLRGHLIDVVSRP
                     DITVWLDRVHLHKEATGSHLSSQDILVARDADRA"
     gene            2259..>3039
                     /locus_tag="VHM65_07315"
     CDS             2259..>3039
                     /locus_tag="VHM65_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX2705552.1"
                     /translation="MDPCELALAVLRALHTGGVRDVVLSPGSRSAPFALALYGAHVAA
                     DLRLHVRLDERSAGFTALGLSIGSHRPVAVVTTSGTAVGNLLPAVMEAHHSGRPLIVV
                     SADRPDRLRGTGANQTTEQAGIFGGFAPCEDLRQGTDPAVVAQAALAACARTGPTQLN
                     IQLDGLLVPTGAPWWPGADPQVEGAIAAPGRRRPTVTTVLGSGPRTVVVAGDDAGPAA
                     RLLAEKAQWPLLAEPTSGARAGPAAIRTYRLLLGTHLRAQIE"
BASE COUNT          373 a         1154 c         1095 g          417 t
ORIGIN      
        1 gacgccgtcg aacatcgcgg cgacgtcacc gggtctcttg tccaggctgg cgcgggtcat
       61 ggccccatcc tcccccaacg gccttgcacc gccatacgcc ggcaggggtg ccacggacgt
      121 aggctgaacg ccgatgtcca ccaggcccga ccccgtgacg ctccgcggcg agcgcgacgc
      181 tgccgtgccc gccgaccctg gtgtccaccc gctcgtcgtg cgcacggtcg agatcaccga
      241 ccccggccca ctgctctcgc tgctgccgga cctggtgccg gatctgttgc cggatctcgg
      301 gccggccggc gaggcgccag ggctggctgc gtgggtgcgc cggggcgagg ggctggtcgg
      361 ctggggtcag gccgcctcgt tcactgcctc cgggccggac cggttcgccg ccgccgacgc
      421 ctggtggacc gccctgtgca gccgcgcggt cgtccgcgac gaggtcgggc tcggcggaag
      481 cggcccggtc atgttcgggt ccttcccgtt tgcggcgggg tcggccgagc cgggccgcgt
      541 cgtcgtccct gaggtcgtca tcggccaccg cggggcccgc tggtgggtca cgacggcgag
      601 cctgggcgcc ggtgtcccct cgatcggggc ggtgccgggg ccgatcgttg ccgcccctcc
      661 gcgctcaccc gtcggcgtga cgttcgccga cggtgcgctc tcaccgaccg aatgggaggg
      721 cgccgtcggc gaggccgtcg tacggatcac gtccggggcg ctggacaagg tcgtcctggc
      781 ccgtgacctg caggccctcg ccaccgaggc gctcgacccg cgctggctgc tgacacggct
      841 cgccgaacgc tactccacga cgtgggtctt cacggtcgcg ggcctgctcg gcgcgacgcc
      901 ggagatgctc gtgcggcgcg acaagggcct ggtcacctca cgggtgctcg ccgggacgat
      961 ccggcgcacc ggcgacgacg ccaaggacct cgcgctcgcc ggctccctcg cccggtcgag
     1021 caaggacctc gaagagcacg agtatgccgt gcgctccgtc gccgatgccc tggcccccca
     1081 ctgctcctcg atcaacgtgc ccgagtcccc gttcgtcctg cacctggcga acgtcatgca
     1141 cctggcgacc gacgtcgcgg gggtggcggc cggctcggcc agcgccctgg agctggcggc
     1201 cgccctgcac ccgtcagcgg ccgtctgtgg cactccgacc gaggtggccg ccgggctcat
     1261 ccacgagctc gagcacatgg accgcggccg ctactccggc ccggtcggct ggatggacgc
     1321 ctccggggac ggtgagttcg ggatcgccct gcgctgtggc cagatcgacg ggcgcgaccc
     1381 gcgcgcaatc cgcctgtatg cggggtgcgg catcgtcgcc gggtccgacc ccgaggccga
     1441 gctggccgag tccgacgtca agctcgtccc catgcgcgac gccctcacca gcgcctgacc
     1501 ccgtcgcccg ggcgcgttgg gccggcgtca gcccaggctg gggacgagga acgggcagag
     1561 cggaccggtg gcaccggcca gccgccggtc gagggtcgcc agcgggcagc ccagtgcctc
     1621 ggcgagcgcg acataccagg cgtcgtacga cgtgaggttc gcccgcagcg cccatgcgcg
     1681 cccggcgaag ggaaggtagg gcaccaggga gaccgggagg tccagcagat ccatgtgggc
     1741 gagcgtggcc gagtccgccg tgatgagccc cgcggcctgc gatcggcgca ggatgttcgc
     1801 cacctccacc ggcatgagat gcggcgcgac gaggtcgtgc ttcgacagga cggacgccgc
     1861 ccaggagcct gtggccccgc cgtcgacgag tgcgccgacg accactgcgc tatcgacgac
     1921 gaccgtcatg cccggtctgc atcgcgggca accaggatgt cctgcgagct caggtgtgac
     1981 ccggtcgcct ccttgtgcag gtgcacccga tcgagccaca cggtgatgtc cggccgcgac
     2041 acgacgtcga tgaggtggcc ccggaggtac tcctgcagcg agcgcccact gccagcagcc
     2101 cgtgccgcca gctcgcgcac cacgtcgtca gggacgtccc gcacagtgat cgacttgctc
     2161 atgcatgcat attagcagca tcacccgcat ttcggcatct cggctgcagc ggcgacctcc
     2221 ggcactcggg gggggcgggc gcggcatact gggtggccgt ggacccctgc gagctggcgc
     2281 tggccgtgct gcgggccctg catacaggcg gggttcgcga cgtggtcctc tcgccggggt
     2341 cgcgctcggc ccccttcgcc ctcgccctgt atggcgcgca cgtcgccgcg gacctgcggc
     2401 tgcacgtccg cctcgacgag cgcagcgcgg ggttcaccgc cctcggcctg agcatcggct
     2461 cgcatcggcc cgtcgccgtc gtgacgacct ccgggaccgc cgtcggcaac ctgttgcccg
     2521 ccgtcatgga ggctcatcac agcgggcgcc ccctcatcgt cgtcagcgcc gaccggccgg
     2581 accgcctgcg cgggacgggc gccaaccaga cgaccgagca ggccgggatc ttcggcgggt
     2641 tcgcgccgtg cgaggacctg cgacagggga ctgacccggc cgtcgtggcg caggccgctc
     2701 tggcggcgtg cgcccgaacc ggtccgaccc agctcaacat ccagctcgac ggcctgctcg
     2761 tccccaccgg agcgccctgg tggccgggtg ccgacccgca ggtcgagggt gccatcgcgg
     2821 cccccggacg gcgccgcccg acggtgacca ccgtgctcgg ctccggacct cgcacggtcg
     2881 tcgtggccgg tgacgacgcg ggcccagccg cccggctcct cgccgagaag gcgcagtggc
     2941 ccctgctcgc cgagcccacc tcgggggccc gggccggtcc ggcggcgatc cggacctacc
     3001 ggctgctgct cgggacccac ctgcgcgcgc agatcgagc
//
LOCUS       DATAIX010000486         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_86301, whole genome shotgun sequence.
ACCESSION   DATAIX010000486 DATAIX010000000
VERSION     DATAIX010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86301"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VHM65_07320"
     CDS             <1..477
                     /locus_tag="VHM65_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705553.1"
                     /translation="DVARLRRGGAVMTTDPELPEEADDYRRKARAFRSELDALPAAER
                     QRAFVDSGYANPHFATPFGRGAGPVEQLVCDTELAGLQGPQLGIGSWIVPTLIQHGTP
                     EQLDRWIWPTLPGSCASVSCSANPRPDPMRRPSARPHGAPTVAGSSRARRSGPLTR"
     gene            complement(1168..>2161)
                     /locus_tag="VHM65_07325"
     CDS             complement(1168..>2161)
                     /locus_tag="VHM65_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2705554.1"
                     /translation="SWVYDKYATVFVQWGQIGSADTTLGPVVTGFVERYSLRLAQRLV
                     EAGMPEGEASEIAGALLVFVNRVHYYRHVGAVRPMPEDEVIDSLALIVQLMLFPTTPL
                     DVLRTIEVTPSPRRARVARPITLDLLRFEGLSARANRTVDRLLHAGSRQFVEVGYSRS
                     TVEDVVIRAEVARGTFYKYFNDKLDLLTVLADEAGQALPTFAERLHDALHADDRHASL
                     RACLRDFEPVHARYAGVLRAWIEQQPDDAHVTAVGQEVKRRWAEAFDSALYSVERGYA
                     LDLNVASLMLLAILERTFDGLPQLTAFGELNRLVELAATLIERGLLNPGSVAAA"
BASE COUNT          335 a          758 c          761 g          307 t
ORIGIN      
        1 gacgtggccc ggctcaggag gggcggtgcc gtcatgacga cggaccccga gctccccgag
       61 gaggccgacg actaccgcag gaaggcgcgg gccttccgca gcgagctcga cgctctcccc
      121 gcggccgagc gccagcgggc gttcgtcgac tcggggtatg ccaacccgca ctttgccacg
      181 ccgttcggcc gcggcgccgg acctgtcgaa cagctcgtct gcgacaccga gctcgccggt
      241 ctgcaaggtc cgcagctcgg gatcgggtca tggatcgtcc cgaccctcat ccagcacggg
      301 acgcccgagc agctcgaccg atggatctgg ccgactctgc cggggagctg cgcttctgtc
      361 agctgttcag cgaacccgag gccggatccg atgcggcggc catccgcacg gccgcacggc
      421 gcaccgacgg tggctggctc atcacgggcc agaaggtctg gaccactgac gcgctgagct
      481 gcaaccgcgg actgatgacg gtccggaccg acccggaggc acccaaacac gccggggtct
      541 cgatgatggt gctcgacctg cgagcgtccg gggtcgacat ccgtcccctt cggggacctc
      601 accggagcgg ccttgttcaa cgaggtgttc ctcgacgacg tgttcgtgcc ggaccgcgac
      661 gtcgtcggtg gggtcgggca gggctggacg gtcgcacgct ccacgctggg caacgagcgg
      721 gtgacgatcg ggcgaggcat gggcggcacc gacctcaccg ccgccatgat cctcgacctg
      781 cacgagcgct atgcgcccgg cgacgacgcg ctcctgtgcg aggtgggccg gatcctagcc
      841 gaggaggcgg cgatggcgct gctcaaccta cgccacgtcg aacggctcat ctccggcggc
      901 tcgatcgggg ccgaggggaa catcaccaag ctgctctcgg ccgagcatgc gcagcgcgtc
      961 accaacctgg ccctgcgggt ggtcggggcg ccggcggtcg gtggcgccga gccccaggtc
     1021 gtcgaggcga tgctctacgc ccgcgggctg tccatcggtg gggcacgtcc gagatcagcc
     1081 gcaaccagat cgccgagcgg ctccttcgcc tccctcgcga cccgctcact cgctaggcga
     1141 accccacact gcggcgcccg cagccggtca ggccgcagcg accgaccccg ggttgagcag
     1201 gccccgctcg atgagggtcg ccgcgagctc gacgagccgg ttcagctcac cgaacgccgt
     1261 gagctgcgga agtccgtcga aggttcgctc gaggatggcc agcagcatga gcgaggcgac
     1321 gttgagatcg agggcgtagc cccgctcgac gctgtacaga gcgctgtcga acgcctcggc
     1381 ccagcgccgt ttgacctcct gccccaccgc cgtcacgtgc gcgtcgtcgg gctgctgctc
     1441 gatccaggcc cgcaggaccc cggcataccg ggcgtgcacc ggttcgaagt cgcgcaggca
     1501 ggcccgcagg ctcgcgtggc ggtcgtcggc atgcagcgcg tcgtggagcc gttcggcgaa
     1561 ggtgggtagt gcctgcccgg cctcgtcggc gagcaccgtg agcaggtcga gcttgtcgtt
     1621 gaagtacttg tagaaggtgc ctcgcgcgac ctcggcccgg atcacgacat cctcgaccgt
     1681 ggaacggctg tagccgacct cgacgaactg gcgggacccc gcgtgcagga gccggtcgac
     1741 cgtgcggttc gctcgtgcgc tgagcccctc gaaacgcagg agatccagcg tgatcggacg
     1801 ggcgacgcgc gctcgacgcg gtgacggcgt gacctcgatg gtgcgcagga cgtcgagcgg
     1861 tgtcgtcggg aacaacatga gctgcacgat gagcgcgagc gagtcgatga cctcgtcctc
     1921 gggcatgggc cggacggcac cgacgtggcg gtagtagtgc acgcggttga cgaagacgag
     1981 cagcgcaccg gcgatctcgc tcgcctcacc ctcgggcatc cccgcctcga ccagccgctg
     2041 ggccagccgc aggctgtacc gctcgacgaa gccggtgacc accggcccca aggtcgtgtc
     2101 ggcagacccg atctggcccc actggacgaa gaccgtcgcg tacttgtcgt agacccagga
     2161 c
//
LOCUS       DATAIX010000487         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_26029, whole genome shotgun sequence.
ACCESSION   DATAIX010000487 DATAIX010000000
VERSION     DATAIX010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26029"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..363)
                     /locus_tag="VHM65_07330"
     CDS             complement(136..363)
                     /locus_tag="VHM65_07330"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX2705555.1"
                     /translation="MLISGKGQVTIPKPLRDALGLEPGSEVEVVLAGDALLLRKFRRS
                     DSRGHRVAGRLRGRGGIMMKTDDIMALTRGD"
     gene            complement(488..1474)
                     /locus_tag="VHM65_07335"
     CDS             complement(488..1474)
                     /locus_tag="VHM65_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX2705556.1"
                     /translation="MTAIVQDGYGAAPETVLRLDQAARPQVADDRVLVRVRAASVDRG
                     TVHLMTGLPYLGRAFMGLRRPKALNPGRCLSGIVEAVGASVTAFRPGDEVYGSSAGSF
                     AEFASARADLLAPKPANLSFEEAAAAPISGVTALQAVRDRAKIEPGQRVLIVGASGGV
                     GTYAVQLAKAFGAEVTGVCSTGKVDLVRSLGADHVIDYTRDDVTGAGRAYDAILDIGG
                     NRPVSQLRRVLTPKGRLVIVGGETDGRWLGGLDRQLRAHLLSPWVSQTLGTFVASASS
                     ADLTVLRDLLESGKVTSAVDRTYPLREVPLAMRYVAEGRARGKVAITVSDRA"
BASE COUNT          231 a          578 c          629 g          246 t
ORIGIN      
        1 acccaggcgg gtccggaccc gcctgggtcg ggcccggcgc tccggcctgt ccggacggac
       61 acgcggacag cgggcgccgg taggcagccc tccccgtcga gggactacgc gtccggctcg
      121 atgacgggct ggggctcagt caccacgggt gagcgccatg atgtcgtcgg tcttcatcat
      181 gatgccgcca cggccgcgca gccgcccggc aacgcgatgg ccgcgactgt cgctccgccg
      241 gaacttgcgg agcaagagcg cgtcgccggc cagcacgacc tcgacctcgc ttccgggctc
      301 gaggccgagc gcgtcacgga gcggcttcgg gatggtgacc tgtcccttgc cactgatcag
      361 catggtagga atcctcccac tcctgctccg gctcctggcc atgccgtccg gagaccagcc
      421 gggcggcaca ggcggctgtc cgcacccgcc cgggccggca ccctgcggta tggcgtggcg
      481 gggctggcta cgctcggtcg ctcaccgtga tggcgacctt gccgcgggcg cgaccttcgg
      541 cgacataccg catggcgagc gggacttcgc ggagagggta ggtccggtcg acggcggagg
      601 tgacctttcc cgactcgagg aggtcgcgca ggaccgtgag gtccgcggag ctcgccgacg
      661 cgacgaaggt gcccagggtc tggctcaccc acggcgacag caggtgcgcc ctgagctggc
      721 ggtcgaggcc gccgagccag cgaccgtcgg tctcgccccc gacgatgacg agccggcctt
      781 tgggggtgag gacccggcgc agctgggaca ccggtcggtt gccgccgatg tcgaggatcg
      841 cgtcgtaggc gcgtcccgcg ccggtgacgt catcgcgcgt gtagtcgatg acgtggtcgg
      901 cgccgaggga ccggaccagg tccaccttgc ccgtgctgca cacgcccgtg acctcggcac
      961 cgaacgcctt ggcgagctgc acggcatacg tcccgacccc gccggacgca ccgacgatga
     1021 ggacgcgctg gccgggctcg atcttggccc ggtcgcgcac ggcctggagg gcggtgaccc
     1081 ccgagatcgg cgcggcggcc gcctcctcga aggagaggtt ggccggcttc ggggccagga
     1141 ggtctgcgcg tgcggacgca aactcggcga aggacccggc gctcgagccg tagacttcgt
     1201 cgcccggccg gaaggcggtg acgctcgcgc cgaccgcctc gacgattcct gagaggcagc
     1261 ggccggggtt gagtgccttg ggtcggcgta ggcccatgaa ggcgcgcccg aggtagggca
     1321 ggccggtcat gaggtgcacc gtgccccggt ccacgctggc cgcgcggacc cgcacgagga
     1381 cccggtcgtc ggcgacctgg gggcgcgcgg cctggtcgag gcgaagcacg gtctcggggg
     1441 cggcgccata cccgtcctgg acgatggccg tcatggttgc agcgccaggc gtggccggcg
     1501 aggggttagt cgggcggtcg aggggcgtgg tcatgatgct ctccagacgt acgttgtaag
     1561 ttgcgtgctt ggagactatg cttacgccgt aagtttgtca atgccgtcgt caggtgacca
     1621 ggtgacgccc tccactcagc ctcaaaccga gtctcgtatg ccgctcaccc gcgcccgcgt
     1681 gctg
//
LOCUS       DATAIX010000488         1670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_56419, whole genome shotgun sequence.
ACCESSION   DATAIX010000488 DATAIX010000000
VERSION     DATAIX010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56419"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..>1670
                     /locus_tag="VHM65_07340"
     CDS             147..>1670
                     /locus_tag="VHM65_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2705557.1"
                     /translation="MSEAATDRTYAQVYAASIEDPEGFWGDAARDIDWITPPTRVLDD
                     SGPPFYRWFPGATLNTCYNALDRHVENGRADQVALHYDSPVAGVKRSYTYAEVLGEVR
                     RLAGALAGAGVGKGDRVVVYMPMVPEAAFAMLACARLGAIHSVVFGGFAPAELAARID
                     DAKPKIILSASCGIEPNRVVPYKPFLDEGIARSEHKPDLVVVLQREQGRAPLGERDVD
                     WQEFVGSAAGDAGADCVEVEATDPLYILYTSGTTGKPKGIYRDNGGHAVALRWSMHNI
                     YNVEPGETMFTASDVGWVVGHSYIVYAPLLTGATTILYEGKPVGTPDAGAFWRLVAEY
                     GAVTMFTAPTAIRAIKKDDPNGEALGDHDLSCLRTLFLAGERLDPDTYHWATDMLGVP
                     VIDNWWQTETGWPIASNPRGLEALEIKPGSPSVCVPGYQVHVLDPAGKEVPAGTEGAI
                     AIKLPMPPGTLPTLWNDDARYVSSYLSAFDGYYLTGDGGFIDEDGYVYVMGRTDDVLN
                     "
BASE COUNT          255 a          609 c          565 g          241 t
ORIGIN      
        1 tcgtccccgc gctcgcgggc cggtggtagc ggccgccgct gcgggccggg ccccggcgga
       61 ccgttcggcg cagcgccgtg cgtccagggc gcacctgcgc cccgcccaac tggcgagcgg
      121 ggccgccccg gggcacagtt cagtccatga gcgaggcagc taccgaccgg acgtacgcgc
      181 aggtctacgc cgcaagcatc gaggacccgg aggggttctg gggtgacgcg gccagggaca
      241 tcgactggat caccccgccg acgagggtgc tcgatgactc cggcccaccg ttctaccgat
      301 ggttccccgg cgcgacgctc aacacgtgct acaacgcgct cgaccgtcac gtcgagaacg
      361 ggcgcgccga ccaggtcgcg ctgcactacg actctcccgt cgccggggtc aagcgctcct
      421 acacgtatgc cgaggtgctg ggtgaggtgc gtcgcctcgc cggagccctg gccggggccg
      481 gggtgggcaa gggcgaccgg gtcgtcgtct acatgccgat ggtgcccgaa gcggccttcg
      541 ccatgcttgc ctgcgcccgg ctcggggcga tccactcggt cgtcttcggc gggttcgccc
      601 cggccgagct ggccgccagg atcgacgacg ccaagcccaa gatcatcctg tcggcatcgt
      661 gcgggatcga gcccaaccgg gtcgtcccgt acaagccgtt cctcgacgag ggcattgccc
      721 ggtccgagca caagcccgac ctcgtcgtgg tcctgcagcg cgagcagggc cgtgccccgc
      781 tcggggagcg agacgtcgac tggcaggagt tcgtgggcag tgccgccggc gacgccggtg
      841 ccgactgcgt cgaggtcgag gcgaccgacc cgctctacat cctctacacc tccggcacga
      901 ccgggaagcc caagggcatc taccgcgaca acggcggaca cgcggtcgcg ctgcgctggt
      961 cgatgcacaa catctacaac gtcgagccgg gcgagacgat gttcacggcc agcgacgtcg
     1021 ggtgggtcgt cgggcactcc tacatcgtct atgcgccgct gctcaccggg gcgacgacga
     1081 ttctctacga gggcaagccg gtcggcaccc ccgacgccgg agccttctgg cggctcgtgg
     1141 ccgagtacgg cgccgtgacg atgttcaccg caccgacggc catccgggcg atcaagaagg
     1201 acgaccccaa cggcgaggcg ctcggcgacc acgacctctc gtgcctgcgc acgctcttcc
     1261 tcgccggtga gcggctcgac cccgacacct accactgggc caccgacatg ctcggggtcc
     1321 cggtcatcga caactggtgg cagaccgaga ccggctggcc catcgcgtcc aacccccgcg
     1381 gcctggaggc tctggagatc aagccggggt caccctcggt ctgcgtgccg ggctaccagg
     1441 tccatgtcct cgaccccgcc ggcaaggagg tcccggccgg cactgagggc gcgatcgcga
     1501 tcaagctccc catgcctccc gggacgctgc ccaccttgtg gaacgacgat gcccggtacg
     1561 tctcgtccta cctgtcggcg ttcgacgggt attacctcac cggagacggc ggcttcatcg
     1621 acgaggacgg gtatgtctac gtcatgggcc gcaccgacga cgtcctcaac
//
LOCUS       DATAIX010000489         3219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_279773, whole genome shotgun sequence.
ACCESSION   DATAIX010000489 DATAIX010000000
VERSION     DATAIX010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279773"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VHM65_07345"
     CDS             <1..623
                     /locus_tag="VHM65_07345"
                     /inference="COORDINATES: protein motif:HMM:NF044952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705558.1"
                     /translation="GRHGLGMSDQIENRIIEAKSRGIYEAPGMALLFLTYERLLNAVH
                     NEDTLANYHAQGRRLGRLLYEGRWLDPQSLMLRESIQRWVASAVTGEVTVRLRRGEDY
                     SILDTRGENLSYHPDKLSMERTEDAAFGPVDRIGQLTMRNLDIADSRAKLELYAAQGQ
                     LLAGHAELVGQLEPGGARAISANPSAANVDAEDDLLDAAAMEFGVD"
     gene            689..1918
                     /gene="rocD"
                     /locus_tag="VHM65_07350"
     CDS             689..1918
                     /gene="rocD"
                     /locus_tag="VHM65_07350"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HEX2705559.1"
                     /translation="MGNLTSHPTSSTADHIDVTETYAAHNYHPLHVVLHEGHGAWVTD
                     VEGRRYLDCLAGYSALNFGHCHPRLVARAQEQLARLTLTSRAFYNDQLGPFAEALARL
                     TGKDMILPMNTGVEAVETALKVARKWGHEIKGVAPNMANIVVLEGNFHGRTTTVVGFS
                     TDETARAGYGPFTPGFRIVPFADAGAVEAAVDENTVAVLLEPIQGESGVLIPPDGYLR
                     DVRRICTSHGVLMLADEIQSGLARTGRTFACDHEDVIPDVYILGKALGGGLLPVSAIA
                     ADLDVLKVMTPGTHGSTFGGNPLAAAVGHEVVLMLETGEYQERAARLGALMREQLDAL
                     VGHGLLAVRSRGLWAGLDIDSATVTGRQLCEAMASRGILAKDTHGSTIRLAPPIVATE
                     EDITVMTSVIAESLATC"
     gene            1991..3193
                     /locus_tag="VHM65_07355"
     CDS             1991..3193
                     /locus_tag="VHM65_07355"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2705560.1"
                     /translation="MATPRQQASDATLRNVERSAGELSTAAIRKMETSHDWFRTLPPE
                     ERSWVGLVAQAGIGAFITWFRGHGGDGPVTASVFGTAPRELTRSVSLGQTLDLVRSVV
                     DVVEEQVSALAAPGDEVVVREAILRYSREIAFAAAEVYAGAAEARGAWDARLEALVVD
                     AVLRGEADDSMQSRATALGWGSVASVTVAAGAASETDTNSAVDTFRKAAARHGVEALA
                     AVQRHRLVVIIGGTTTPMRVLTSLLSHFGEGPVVLGPTVPHLFAAGRSARAALSGLGA
                     AAAWPEAPRPVYADDLITERVLAGDVHAQRALISRVFQALEGNPSLLDTASVFLDTGS
                     LEGTARMLFVHPNTVRYRLGRISEITGYDLTHPRCVGLALGRLARPAPTSWRAGVPGR
                     SRTGSEEL"
BASE COUNT          474 a         1204 c         1105 g          436 t
ORIGIN      
        1 gcggccgcca cggcctcggc atgtccgacc agatcgagaa ccgcatcatc gaggccaaga
       61 gccgcgggat ctacgaggcg ccgggtatgg cgctgctctt cctcacctac gagcgcctcc
      121 tcaacgccgt gcacaacgag gacacgctcg ccaactacca cgcgcagggg cgccggctag
      181 gccggctcct ctacgaaggc cgctggctcg acccgcagtc gctcatgctg cgcgagtcga
      241 tccagcgctg ggtcgcctca gcggtgacag gcgaggtcac cgtgcggctg cggcggggtg
      301 aggactactc gatcctcgac acccgcggcg agaacctctc ctaccacccg gacaagctgt
      361 ccatggagcg caccgaggac gcggccttcg ggcccgtcga ccggatcggt cagctgacga
      421 tgcgcaacct cgacatcgcc gacagccggg ccaagctcga gctgtatgcc gcccagggcc
      481 agctgctcgc gggacacgcc gagctcgtcg ggcagctcga acccggtggg gcgcgcgcga
      541 tctcggccaa cccgtcggcg gcgaacgtcg acgccgagga cgacctgctc gacgcggccg
      601 ccatggagtt cggcgtcgac tgacccgcgg gacccctgtg cgtgccgtgc cgcccagtgc
      661 ccgtacgatc gagcccggaa ggagggccat gggcaacctc acgtcccacc cgacgagctc
      721 gaccgccgac cacatcgacg tcaccgagac ctacgcggcc cacaactacc acccgctgca
      781 cgtcgtgctg cacgagggtc acggcgcctg ggtcaccgac gtcgagggcc ggcgctacct
      841 cgactgcctc gcgggctact cggcgctgaa cttcgggcac tgccatcccc ggctcgtcgc
      901 gcgggcccag gagcagctcg cccggctcac cctgaccagc cgggcgttct ataacgacca
      961 gctcggcccg ttcgccgagg ccctggcccg gctcaccggc aaggacatga tcctgccgat
     1021 gaacaccggc gtcgaagccg tcgagacggc cctcaaggtg gcccgcaagt gggggcacga
     1081 gatcaagggc gtggccccga acatggccaa catcgtcgtc ctggagggga acttccatgg
     1141 ccgcacgacg accgtcgtgg gcttctccac cgacgagacc gcccgcgcgg gctacgggcc
     1201 gttcaccccg ggattccgga tcgtcccgtt cgccgacgca ggagcggtcg aggccgccgt
     1261 cgacgagaac accgtggccg tgctgctcga gccgatccag ggcgagagcg gcgtcctcat
     1321 cccgcccgac ggctacctgc gcgacgtccg gcgcatctgc accagccacg gcgtgctcat
     1381 gcttgccgac gagatccagt ccgggctggc gcgcaccggc cggaccttcg cctgcgacca
     1441 cgaggacgtc atcccggacg tatacatcct gggcaaggcc ctcggtggcg gactgctccc
     1501 cgtatcggcg attgctgcgg acctcgacgt tctcaaggtc atgaccccgg gcacgcacgg
     1561 ctcgaccttc ggcggcaacc cgctggcggc ggccgtcggc cacgaggtcg tgctcatgct
     1621 cgagaccggt gagtaccagg agcgcgccgc ccggctcggt gccctgatgc gcgagcagct
     1681 cgacgcgctg gtgggccacg gcctgctcgc cgtccgctcc cgcggcctct gggcgggcct
     1741 ggacatcgac tcggccacgg tcaccgggcg ccagctgtgc gaggcgatgg cgagccgcgg
     1801 catcctcgcc aaggacaccc acggatcgac gatccggctc gcgccgccca tcgtggcgac
     1861 ggaggaggac atcaccgtga tgaccagcgt catcgccgaa agcctggcca cctgctgagc
     1921 cgccggccac ccggcaggac tgcccgcctc ggcgggcgct ttgtgcgcgc catacaaata
     1981 ccatgtccgt atggcgacgc cccggcagca ggcgagcgac gccaccctgc gcaacgtcga
     2041 gcgcagcgcg ggggagctgt cgacggcggc gatccgcaag atggagacga gccacgactg
     2101 gttccggacc ctgccgccgg aggagcggtc gtgggtcggg ctcgtcgccc aggccgggat
     2161 cggggcgttc atcacgtggt tccgcggcca cggcggcgat ggcccggtca ccgccagcgt
     2221 cttcggcacg gcaccgcgtg agctgacccg ctcggtaagc ctgggacaga cgctcgacct
     2281 ggtccgctcg gtcgtcgacg tcgtcgagga gcaggtcagc gcgctggccg ctcccggtga
     2341 cgaggtcgtc gtccgcgagg ccatcctgcg ctactcgcgc gagatcgcct tcgccgcggc
     2401 cgaggtgtat gccggggccg cagaggcccg cggcgcgtgg gatgcccgcc tcgaagcgct
     2461 cgtcgtcgac gcggtcctgc gcggtgaggc ggacgactcg atgcagtcgc gagccaccgc
     2521 actcgggtgg ggatcggtcg cgtcggtgac cgtcgcggcc ggtgcggcct cggagaccga
     2581 caccaactcg gccgtcgaca cgtttcgcaa ggccgcagcc cggcacggag tcgaggcgct
     2641 ggccgccgtc cagcggcacc ggctcgtcgt catcatcggg ggcacgacca caccgatgcg
     2701 ggtgctcacc tcgctgctga gccacttcgg ggaagggccg gtcgtcctgg gtcccacggt
     2761 gccgcacctg ttcgcggcgg gccgctccgc gcgggcggcc ctgagcgggc tcggggctgc
     2821 ggccgcgtgg ccggaggcac cgcgcccggt ctacgccgac gacctgatca ccgagcgcgt
     2881 cctggccggg gacgtccatg cccagcgggc cctcatcagc cgtgtcttcc aggccctcga
     2941 ggggaacccg tcgctgctcg acacggcctc ggtcttcctc gacacggggt cgctcgaggg
     3001 caccgcgcgg atgctcttcg tccaccccaa cacggtccgc taccgcctcg gccggatcag
     3061 cgagatcacc ggctacgacc tcacccatcc gcgctgcgtg ggcctcgcgc tgggccgcct
     3121 ggcccggcct gctccgacca gctggcgggc aggcgtccct gggcgttccc gcacgggaag
     3181 cgaggaactg taggaaccct ccaagcgtgc cctccatag
//
LOCUS       DATAIX010000490         5349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_86506, whole genome shotgun sequence.
ACCESSION   DATAIX010000490 DATAIX010000000
VERSION     DATAIX010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86506"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(537..896)
                     /locus_tag="VHM65_07360"
     CDS             complement(537..896)
                     /locus_tag="VHM65_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705561.1"
                     /translation="MREAFAHEAVLTLSAGADTAALGAAVTMALCGHWEHEPPCPLAP
                     HHSAVTEAGDDVVLRTLFAVEPTGESEVRARITEALAGGQLAGSEDTTTRWRLRSSAP
                     SVVAAGEREHAERLTNS"
     gene            complement(1141..2133)
                     /locus_tag="VHM65_07365"
     CDS             complement(1141..2133)
                     /locus_tag="VHM65_07365"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX2705562.1"
                     /translation="MDTGTLPGAAVDSARTPYSSVVRSNQARATRRAIVTAAADLFVE
                     QGYAATTIDAVAARARVGRKTVFTVGGKGALLKLAWDWALTGDDEPIPMSERPAVQAM
                     LAERDPRRLVRMWVDMLLDVGSRAAPIGAVVLAAADVDLEARELRDMIRRESLAGATA
                     FVTHLAGAGGLRPGVSVERGADACWALVNSLLLDLVVATRGWSLQEYGEWLVRLASAT
                     LLEPEGGPASSQRPPAVRIVHAATRERYEASVDGRAAGHLSYQGTGRLVVLTHTEVEP
                     GFEDRGVADALVRRALDDLRADGARRVVPLCHYVAWWVGRHPGYAPLVYDAAAD"
     gene            2173..3585
                     /locus_tag="VHM65_07370"
     CDS             2173..3585
                     /locus_tag="VHM65_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2705563.1"
                     /translation="MSVVTTLAVRDEVVMQVAIIGAGPAGLLAGSALAGRGHDVAVVD
                     RDAGPPAHAGWARRGVMQFHHAHGFRPQVGQVLHEQWPAALETWRALGAEPITLDVPG
                     LGAVPGGHRSRRETFERALRMASHAVPGLSVRQGHVDGVLSSGGRVHGVMVDGARLEA
                     DLVVDASGRSGRATDELRAPSTVGGPCGMAYVDRQYRLRDGAEPGPMANPLAWQADFD
                     GYQTIVFLHERGHFSVLLVRPTADAALKALRHEAAFEAACRAIPGLAEWTDTKRALPV
                     TGVLVGGPLRNTYRGQRGVDGRSVLPGLVSVGDSVATTTPTFGRGVATTFLQVRQLLA
                     LVDASADPALVGEPFDAWCEANMLPWVLDHTHMDGDLVRRWQGGGVDLTRRLPSDLIL
                     SAATVDPRIGQAAPGYLSMRELPSCLDPVEPLARAVYESGWRPAYSPGPTRDELVDII
                     DAAIREGGPEAARADSSQAG"
     gene            complement(3813..4358)
                     /locus_tag="VHM65_07375"
     CDS             complement(3813..4358)
                     /locus_tag="VHM65_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705564.1"
                     /translation="MNPSTRPLVLGRGGRTPQAPAQPSAPLVNPPGVPVEGLSEATTF
                     TIEGLPRNDNGKALVTVGWPDASVDWDVYVLDAAGELVSSAASLDDPEVALMFDPVPG
                     TYTVIVENYAGGSAATDWTGSVDFAQPTPAYDSGVREAWTLTCRNATGRVLGTQLVIV
                     GRGQSTDVGDPCVRSRAKGTR"
     gene            complement(4470..4964)
                     /locus_tag="VHM65_07380"
     CDS             complement(4470..4964)
                     /locus_tag="VHM65_07380"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX2705565.1"
                     /translation="MADSSFDVVSKYDKQEVANAVNTAAKEIANRYDFKHVGASIELS
                     GEKILMKANSEERVKAVLDVLQTWLVKRKVSLKHLDGADKEPQLSGKEYRLEVGLKAG
                     ISQDSAKKITKLIRDEGPRTVRALIQGDELRVWSKSKNDLQAVQQMLKEADLDVALQF
                     TNYR"
     gene            5127..5210
                     /locus_tag="VHM65_07385"
     tRNA            5127..5210
                     /locus_tag="VHM65_07385"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5161..5163,aa:Tyr,seq:gta)
BASE COUNT          709 a         1948 c         1902 g          790 t
ORIGIN      
        1 cgcacgggtc gagctccagg aggccgccga cggcacacgc atgacggtca catcgcagtt
       61 cgtgaccctc gagcagatgg agcagctcgt cgcgatgggc atggtcgagg gctcgaccct
      121 cgcgatgggc cagatcgacg agatcctggc cgccgcctga ccgggcgatc gagggcggac
      181 gcgctcccta ccgacaagac cgcgtcggag gcgctcgatt tcgcggccct cgccgtggga
      241 gcgccgccga ccccgtcccc cgcctcgttg agccggccgt gtcggtgaag agcgccgccc
      301 gtggcagctc gtgggccgat gggcgcttgt cgcccgccgt gacgcggcgg gatgctacct
      361 cgcgcgggcc cagtagccca ggtccccggt ggcctcataa tccacggcgg tgtcctcacc
      421 gtgaagctgg gggagcgcgc cgtcggccgc ggggaacccg acccgctgga ggtcggggga
      481 cgagacggtg ccgagcggcg agatgccgcc cgccccgctc ttggtgcggt cgcaggtcag
      541 ctattggtca gccgctcggc gtgctcccgc tcgcccgccg ccaccacgct gggcgcactc
      601 gaccgcagcc gccaccgcgt cgtcgtgtcc tccgagccgg ccagctgacc gcccgccagc
      661 gcctccgtga tccgggcgcg cacctcgctc tcgcccgttg gctcgactgc gaacagggtg
      721 cggagcacca cgtcgtcgcc ggcctcggtc accgcgctgt ggtgcggtgc cagtggacac
      781 ggcggctcgt gctcccagtg accgcatagc gccatcgtca cggcggcgcc cagggccgcg
      841 gtgtcagcac ccgccgacag tgtcaggacc gcctcgtggg cgaaggcttc tcgcacgaac
      901 ccagtgtgcc gcaaggggcc gacgctctcc gctgacctag acaggtgcat gggcttggct
      961 tctgagttcg gcctggccca ggtctgacat ccgatcatcc accgacaccg ccagctcggc
     1021 cgcccaaccg caatgtgcac gtctcgaagt cgggcaacgt ccgctcatcc aggccgaatg
     1081 ggcggatgtc ccccgacctc ggcgggggcg agtggggcgg cagcgagggg tccatgtgcc
     1141 tcagtcggcc gcggcgtcat acaccagggg tgcatagccg gggtgacggc cgacccacca
     1201 cgcgacgtag tgacacaggg gaacgacgcg gcgcgctccg tcagcccgca ggtcgtcgag
     1261 cgccctgcgt accagcgcgt cggccacgcc ccggtcctcg aagcccggtt cgacctcggt
     1321 gtgggtgagc acgaccagcc gcccagtgcc ctgataggac aggtgccccg cggcccggcc
     1381 gtcgaccgac gcctcgtagc gctcgcgcgt cgccgcgtgc acgatccgga ctgccggggg
     1441 gcgttgggag ctcgcggggc cgccctcggg ctcgagcagc gtcgccgagg ccagccgaac
     1501 caaccactcg ccatactcct gcagcgacca gccacgcgtg gccaccacaa ggtccaggag
     1561 cagggagttg accagcgccc agcaggcgtc tgcgccgcgt tcgacgctga cgcccgggcg
     1621 caggccacct gcgccggcga ggtgggtgac gaaggcggtc gctccggcca gggactcgcg
     1681 ccggatcatg tccctgagct cgcgagcctc gaggtcgaca tccgccgccg cgaggacgac
     1741 ggccccgatc ggtgccgccc gcgagcccac gtcgagcagc atgtcgaccc acatccggac
     1801 cagccggcgc gggtcccgct ccgcgagcat cgcctgcacg gcgggtcgct cggacatggg
     1861 gatgggctcg tcgtcgccgg tcagggccca gtcccacgcc agcttgagca gcgcaccctt
     1921 ccccccgacc gtgaagacgg tcttgcgccc gacccgggcc cgtgcggcga cggcgtcgat
     1981 cgtggtggcg gcatacccct gctcgacgaa gagatcggct gccgcggtca cgatggcacg
     2041 ccgggttgcc cgggcctggt tggagcgcac caccgaggag tatggcgtcc gcgcgctgtc
     2101 gacggccgcg cccggcagtg tgcccgtgtc caccgagaat ctccttgacg tcggccagtg
     2161 gttggggaca ccatgagtgt cgtaaccaca ctagcagtgc gagacgaggt cgtcatgcag
     2221 gtcgccatca tcggagccgg cccggccggt ctgctcgccg gtagtgctct ggccggccgc
     2281 ggccacgatg tcgccgtggt ggaccgcgac gccggcccgc cggcgcacgc cggctgggcc
     2341 cgccgcgggg tgatgcagtt ccaccacgcg cacggcttcc ggccccaggt gggccaagtc
     2401 ctgcacgagc agtggcccgc ggcgctcgag acgtggcgcg cactcggcgc cgaaccgatc
     2461 accctcgacg tccccggcct gggagcggtg ccgggcggac accggtcccg ccgggagacc
     2521 ttcgagcgcg cgctccggat ggcctcgcac gccgtgccgg gcctgtctgt gcggcagggg
     2581 catgtggacg gcgtgctgtc ctccggcggg cgggtccacg gggtcatggt cgacggcgcc
     2641 agactggaag ccgacctcgt cgtcgacgcg tccggacggt caggccgggc gaccgacgag
     2701 ctgcgcgccc cgtcgacggt cggcgggccg tgcggtatgg cgtatgtcga ccgtcagtac
     2761 cggctccgcg acggggccga gcctgggccg atggccaacc ccctggcttg gcaggccgac
     2821 ttcgacggct accagaccat cgtgttcctg cacgagcggg gccatttctc ggtcctgctg
     2881 gtccgcccga cggccgacgc ggcactgaag gccctgcgtc acgaggccgc gttcgaggcc
     2941 gcctgccggg ccattcccgg gctcgccgag tggaccgaca ccaagcgcgc cctgccggtg
     3001 accggcgtgc tggtgggcgg accgctgcgc aacacctacc gcgggcagcg cggcgtcgac
     3061 gggcggtccg tgctgcccgg gctcgtcagc gtcggcgact cagtcgcgac caccaccccg
     3121 accttcggtc gtggcgtggc cacgacgttc cttcaggtcc gccagctgct cgccctcgtg
     3181 gacgcgagcg ccgaccccgc cctggtcggc gaaccgttcg acgcctggtg cgaggcgaac
     3241 atgctgcctt gggtgctgga ccacacacac atggacggcg acctcgtccg ccgctggcaa
     3301 ggtgggggcg tcgacctgac ccggcggctg ccgtcggacc tgatcctgtc cgccgccacc
     3361 gtggaccccc gtatcggtca ggccgccccc ggctacctct ccatgcgcga gctgccctcc
     3421 tgcctggacc cggtcgagcc cctggcccgc gccgtctacg agtccggctg gcgcccggca
     3481 tacagccccg ggccgacccg cgacgagctc gtcgacatca tcgacgccgc gatccgcgag
     3541 ggtgggccgg aggcggcacg ggccgactcg agccaggccg gctagctgct catccgctgg
     3601 gacgggagac gcgataccgc aggtgtgtga cgtcgggtgc atcggccaca cgggtcttcg
     3661 tgaggtgcgg acccgcaccg gtgtggtcga ccaacgggat cccggccccc gagacggacg
     3721 gcgacgacgt gcagctgcac ctcgtcatcc gggatcggcg accgcgcgcg agaacgcctc
     3781 gcccgccgcg ggctccccgc ccgccgacag ggtcagcggg tgcccttggc ccggctccgg
     3841 acgcaagggt caccgacgtc cgtgctctgt ccgcgcccga cgatgaccag ctgggtgccc
     3901 aggacccggc ccgtggcgtt ccggcaggtc agggtccacg cctcgcgcac gccggagtcg
     3961 taggcgggcg tcggctgggc gaagtcgacc gacccggtcc agtcggtggc ggccgacccg
     4021 cccgcgtagt tctccacgat aacggtgtag gtcccgggca ccgggtcgaa catgagcgcc
     4081 acctcggggt cgtcgagcga ggccgcggac gagaccagct cgccggccgc gtccaggacg
     4141 tagacgtccc agtccacgct cgcgtccggc cagccgaccg tgaccaacgc cttgccgttg
     4201 tcgtttctcg gcaggccctc gatcgtgaag gtcgtcgcct cgctcaagcc ctcgacgggc
     4261 actcccggcg ggttgaccag aggcgcgctg ggctgagccg gcgcctgagg ggtccgcccg
     4321 ccgcgcccga gaacgagcgg ccgcgtcgag gggttcacgg ccatcgtgaa gcggccaccc
     4381 gtgctggcgt aggccgagcg caggtggtcg gcgagggcaa acggtcgacc cggccggctc
     4441 cagggctggc cgagcggctc cggggcgcgt cagcggtagt tggtaaactg cagcgccacg
     4501 tcgaggtcgg cctccttgag catctgctga acggcctgca ggtcgttctt ggacttgctc
     4561 cacacccgca gctcgtcgcc ctggatcagg gcccgcacgg tgcgggggcc ttcgtcgcgg
     4621 atcagcttgg tgatcttctt ggcgctgtcc tggctgatgc cggccttgag cccgacctcg
     4681 aggcggtact ccttgcccga cagctgcggc tccttgtcgg cgccgtcgag gtgcttgagg
     4741 ctgaccttgc gcttgacgag ccacgtctgc aggacgtcga ggacggcctt gacgcgctcc
     4801 tcggagttcg ccttcatgag gatcttctcg ccggacagct cgatggaggc gccgacgtgc
     4861 ttgaagtcgt agcggttggc gatctccttg gcggcggtgt tgaccgcatt ggccacctcc
     4921 tgcttgtcgt acttgctgac gacgtcgaag gacgagtcgg ccatcgtggg ctccttccag
     4981 gcgtgggtat gacgggccgg tggcggtccc gtggtgggct cgggcggtgg cccgggccgg
     5041 tcgggcggcg ggccccgggg cggaccgcgt tctgttcctg tttcgcctcg gtcccgggtt
     5101 ccttgctatc cttccatgcg caccacggca ggttgcccga gcggccaatg ggagcggact
     5161 gtaaatccgt cgcgaaagct tccaaggttc gaatccttga cctgccacca cctacaagaa
     5221 cggcccctga ccagcacaaa tgccggtccg gggccgttct tctgcgtcca gccatgtccg
     5281 gctgggggcg ggcatctacg gttcttcgtg tcctgcacgg cacgttccgc acctctggag
     5341 ggcaatatc
//
LOCUS       DATAIX010000491         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_86639, whole genome shotgun sequence.
ACCESSION   DATAIX010000491 DATAIX010000000
VERSION     DATAIX010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86639"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1523)
                     /locus_tag="VHM65_07390"
     CDS             complement(<1..>1523)
                     /locus_tag="VHM65_07390"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEX2705566.1"
                     /translation="PRARYNTPVRLKIESLLREAFHAVSVDYTTRVTESSLARLHFVV
                     RVRQGEVIPDVDEPGLTRAIVEAARTWDEDLAEAARAEYGEEGAARLMGLYSRAFPEA
                     YKEDFGPRVAVADVRFIESLTALDSTGLNMYQEPGAPSHERRFKLYRRGPLSLTQILP
                     IFTHMGVEVIDERPYQMVRSDGALIHIYDFGLRAGDAERWGGSAHRDVVRGLFHDAVH
                     AVWSGQAESDGFNALVLGAGLTWRQVVVLRAVAKYLRQTQSRFSQDYVEAALTSNPTI
                     ARRLVVLFETRFDPARFGQDPAGRSAAQASIVASLMSDLDAVKSLDHDRIIRALLGVV
                     TAGLRTNYFQLDAAGAAKSYISLKLNPKEVPDLPAPRPAYEIWVYSPRVEGVHLRFGK
                     VARGGLRWSDRREDFRTEVLGLVKAQMVKNSVIVPTGSKGGFFAKNLPDPAVDREAWL
                     AEGIASYKLLISGLLDLTDNRVSGRIIAPVDLLWNGGIGTYLKASTESDADIGDRAN"
BASE COUNT          233 a          501 c          558 g          231 t
ORIGIN      
        1 tcgttggccc ggtcgccgat gtcggcgtcg ctctccgtgg acgccttgag gtaggtgccg
       61 atgccaccgt tccagagcag gtcgaccggc gcgatgatcc gcccgctgac ccggttgtcg
      121 gtgagatcga gcagaccgga gatgagcagc ttgtatgacg cgatcccctc ggccagccac
      181 gcctcgcggt cgacggcggg gtcgggcagg ttcttggcga agaagccgcc cttggaaccg
      241 gtcgggacga tgaccgagtt cttgaccatc tgggccttga ctagacccag gacctcggtg
      301 cggaagtcct cgcggcggtc cgaccagcgc agcccgcccc gggcgacctt gccgaagcgc
      361 aggtgcacgc cctcgacccg ggggctgtag acccagatct cgtaggccgg ccgtggcgcg
      421 ggcaggtcgg ggacctcctt ggggttcagc ttgaggctga tgtaggactt ggccgcgccc
      481 gcggcatcga gctggaagta gttcgtgcgc agcccggcgg tgacgacgcc gagtagcgcc
      541 cggatgatcc ggtcgtggtc gaggctcttg accgcgtcaa ggtcgctcat gagcgaggcg
      601 acgatcgatg cctgcgccgc cgaacgcccg gccgggtcct ggccgaaccg ggccgggtcg
      661 aaccgggtct cgaacaggac aacgaggcgg cgggcaatcg tggggttgga ggtcagtgcc
      721 gcctcgacat agtcctggga gaaccgggac tgcgtctggc gcaggtactt ggccaccgcg
      781 cgcagcacga ccacctggcg ccaggtgagg ccggcgccga gcacgagtgc gttgaagccg
      841 tcgctttccg cctggccgct ccacacggcg tgaaccgcgt cgtggaacag tccccggacc
      901 acgtcgcgat gggcactgcc gccccagcgc tcggcgtccc cggcccggag cccgaagtca
      961 tagatgtgga tcagggcacc gtccgagcgc accatctggt agggccgctc gtcgatgacc
     1021 tcgacaccca tgtgggtgaa gatcggcagg atctgggtca gggagagcgg gccgcggcgg
     1081 tagagcttga accgccgttc gtgtgagggc gcgccgggct cctggtacat gttgagcccg
     1141 gtcgagtcca gcgcggtgag ggactcgatg aaacgcacgt cggcgaccgc gacccgggga
     1201 ccgaagtcct ccttgtacgc ctccgggaag gcccgggagt acagccccat gaggcgggcc
     1261 gcgccctcct cgccatactc ggcccgggcg gcctcggcca ggtcctcgtc ccacgtccgg
     1321 gcggcctcga cgatcgcgcg ggtgaggccc ggctcgtcga cgtcggggat tacctcacct
     1381 tgccggaccc ggacgacgaa atgcaaccgg gccagcgagg actcggtcac ccgggtcgtg
     1441 tagtcgacgc tgaccgcgtg gaaggcctcg cgcagaagcg actcgatctt gagccggacg
     1501 ggcgtgttgt agcgggcgcg tgg
//
LOCUS       DATAIX010000492         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_86706, whole genome shotgun sequence.
ACCESSION   DATAIX010000492 DATAIX010000000
VERSION     DATAIX010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86706"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..1500
                     /locus_tag="VHM65_07395"
     CDS             307..1500
                     /locus_tag="VHM65_07395"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX2705567.1"
                     /translation="MSGPLAGITVVDLSRALAGPHATMILGDLGARVIKVESPGTGDD
                     TRGYGPPFVGPADEPISTYYLSCNRNKESITLDLKSDDGRETLTRLVRHSDVLVENFR
                     PGVLDREPEPVERLREITPTLVILKISGFGEDGPQGGRAGYDQIAQGEAGLMSLTGSG
                     PDDPQRVGVPIADLLAGHNGAIGVLAALHDRTTTGHGRVVRTSLLAGVVSVHAFQGTA
                     YTVAGEVPRAQGNHHPAIAPYGLFHCSDGILQIACGAEGLWVKLAEAIGIDPAEPGLA
                     TNAERVVNRPLLVERIDARLATRTLEDLMALLARIGVPAGEVRTIDRVYDWDQTRSQH
                     LVIDVEHPVLGRIELPGSPLRFDDLPYSGGRDVHLPPPPLGEHNDAVRAWLDDRDAIA
                     AADRS"
BASE COUNT          243 a          544 c          527 g          207 t
ORIGIN      
        1 tacacataca ttagcatgtg catatcgtca cggtatgaac cccgtccaca aggccctcaa
       61 tccaccaggg ccctgtctcg aaccacgaca gtccgagtgg gcagatcctc atggtgaacg
      121 cggcgcaggg cgaccgcgac cggcagtgag tcacgtggcg acccagggca tacgtcgcgt
      181 ttcggaggtg ccgtgggcac gtgaccttgc ggcgaggagg cgggggtgcc aacgtcaagg
      241 ggtgccgact gcgggcacca ccagcgcgcc tgacccggcg cccggcatac gagagagaag
      301 ggcactgtga gcggtccgct ggctgggatc accgtcgtcg acctgtcgcg agcgctcgcc
      361 gggccgcatg cgacgatgat cctcggcgac ctcggcgcgc gagtgatcaa ggtcgagtcg
      421 ccggggaccg gggacgacac ccgtggctac gggccgccgt tcgtcgggcc ggcggacgag
      481 ccgatctcga cgtactacct gtcgtgcaac cgcaacaagg agtcgatcac cctcgacctc
      541 aagtccgacg acggccgcga gacgctgacc cggctcgtcc gccacagcga cgtgctcgtc
      601 gagaacttcc gcccgggggt cctcgaccgt gaacccgagc cggtcgagcg gctgcgtgag
      661 atcaccccga cgctggtcat cctcaagatc agcgggttcg gcgaggacgg gccgcagggc
      721 ggccgcgcgg gctacgacca gatcgcccag ggcgaggccg ggctcatgtc gctgactggc
      781 tccgggccgg acgacccgca gcgggtcggg gtgccgatcg ccgacctgct cgccgggcac
      841 aacggggcga tcggtgtgct ggccgccctg cacgaccgca cgacgaccgg ccacggccgg
      901 gtcgtgcgca cctcgctgct cgcgggcgtg gtcagcgtgc atgccttcca gggcacggca
      961 tacaccgtgg ccggtgaggt gccccgcgcc cagggcaacc accacccggc gatcgcgccc
     1021 tacgggctgt tccactgttc cgacgggata ctccagatcg cctgcggcgc cgaggggctg
     1081 tgggtcaagc tcgccgaggc catcgggatc gaccccgccg agccgggctt ggccaccaac
     1141 gccgagcggg tcgtcaaccg gccgctactg gtcgagcgga tcgacgccag gctggccacc
     1201 aggacgctcg aggacctcat ggccctgctc gccaggatcg gtgtcccggc cggtgaggtc
     1261 cgcacgatcg accgggtgta cgactgggac cagacccgct cgcagcacct cgtcatcgac
     1321 gtcgaacacc cggtcctcgg ccggatcgag ctgcccgggt cgccgctgcg cttcgacgac
     1381 ctcccctact cgggcgggcg cgacgtgcac ctgccaccgc ctcccctcgg tgagcacaac
     1441 gacgcggtgc gtgcgtggct cgacgaccgc gatgccatcg cagccgcgga ccgatcatga
     1501 gcgacgacag gcctgttcga c
//
LOCUS       DATAIX010000493         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_26410, whole genome shotgun sequence.
ACCESSION   DATAIX010000493 DATAIX010000000
VERSION     DATAIX010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26410"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VHM65_07400"
     CDS             complement(<1..453)
                     /locus_tag="VHM65_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2705568.1"
                     /translation="MTYLPAATRYDAMQYRRSGRSGLQLPAISVGLWQNFGHDRPIDS
                     QRAVIHRAFDLGITHFDLANNYGPPYGSAEENFGRILATDLRGYRDELIISSKAGYDM
                     WPGPYGNWGSRKYLLASLDQSLGRLGLEYVDIFYSHRFDPETPLEETMA"
     gene            complement(582..1214)
                     /locus_tag="VHM65_07405"
     CDS             complement(582..1214)
                     /locus_tag="VHM65_07405"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2705569.1"
                     /translation="MGERTRVANRLHAALVVLAAGYKREVPNLAGACHRAAVARLLDG
                     LAGVRAELARADLARLRELDAECSALEKQIRSLVQLSGSSLPTITGVAAITAAKLMGE
                     TGDPRRIRSTPAFAAMSGTAPIPASSGQTSRHRLNRGGNRQLNRALHTIALVQARTDP
                     RAKAYMARRTAEGKSRLEALRCLKRHLADVVLRTMLADVERSARPVTEGA"
     gene            complement(1207..1626)
                     /locus_tag="VHM65_07410"
     CDS             complement(1207..1626)
                     /locus_tag="VHM65_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2705570.1"
                     /translation="MVTIGIDTHKATLAVSAIDATGRETGARTFPNDRRGHVGILRWA
                     QAHGSERRFGIEGSGSYGAALGRLLVAAGERVVEVPAILTDRERRHLRRAGKSDPGDA
                     LAIARVALREEGLGPVPMPGITEDLETPRRCAGPAGG"
BASE COUNT          252 a          591 c          635 g          286 t
ORIGIN      
        1 ggccatcgtc tcctcgagcg gcgtctccgg gtcgaaccgg tgggagtaga agatgtcgac
       61 gtactcgagg cccagccgac cgagactctg gtcgaggctc gcaaggaggt acttgcgcga
      121 gccccagttg ccgtacggtc cgggccacat gtcgtagccg gccttcgagc tgatgatcag
      181 ctcgtcccgg tagccgcgca ggtccgtggc gaggattcga ccgaagttct cctccgccga
      241 gccgtacggc gggccatagt tgttagccag gtcgaagtgg gtgatgccca ggtcgaaggc
      301 gcggtggatg acggcccgct ggctgtcgat cggccggtca tgcccgaagt tctgccacag
      361 tccgaccgag atggcgggca gctggagccc gctcctcccg gatcgccggt attgcatggc
      421 gtcgtagcgg gtagcggcag gaaggtacgt catcgtccca ttatgttctt ccgcgacagc
      481 caatggggga tacggtccgg ggagccgtgc gagtgtgcga ggggcggttt atgacctggc
      541 gtcgcgacca catcccgcac gatctacagg ctcctgactt gtcaagcccc ctcggtgacg
      601 ggtcgtgcgc tccgctcgac atcggcgagc atggtgcgca gcaccacatc ggcgagatga
      661 cgcttcaggc agcgcagggc ctcgagccgg gacttgccct cggcggttcg tcgcgccatg
      721 tatgccttgg cgcgcgggtc ggtccgggcc tggacgagcg cgatcgtgtg gagggcccga
      781 ttgagctgtc ggttgccgcc tcggttgagg cgatgtcggc tcgtctggcc ggaggaggcc
      841 ggaatgggtg cggtaccgct catcgccgcg aacgccggtg ttgagcggat gcggcgcgga
      901 tcgccagtct cacccatgag cttggcggcc gtgattgcgg ccacgcccgt gatcgtcggc
      961 aggctcgagc ccgagagctg gacgagcgag cggatctgct tctcgagcgc ggagcactcc
     1021 gcatccagct ctcggagccg agcgagatcg gcgcgggcga gctcggcgcg cacgccggcc
     1081 agaccatcga ggagacgggc gaccgctgcg cggtggcagg ccccggcgag gttcgggacc
     1141 tcgcgcttgt agccggcggc cagcacgacc agggccgcgt ggaggcggtt cgcaacccga
     1201 gtgcgctcac ccacccgctg gtcccgcgca tcgacgagga gtttccagat cctcggtgat
     1261 cccgggcatg gggaccgggc cgaggccctc ctcgcgaagg gccacccggg cgatcgcgag
     1321 ggcgtcgcct ggatcgctct tgcccgcccg gcggaggtgc cggcgctcgc gatcggtgag
     1381 gatcgcgggt acctcgacga cccgctcacc cgcggccacg agcagtcgcc ccagggccgc
     1441 gccgtacgag cccgagccct cgatcccgaa gcgccgctcc gagccgtgcg cctgggccca
     1501 gcggaggatc ccgacgtgcc cccggcgatc gttcgggaac gtcctcgccc cggtttcgcg
     1561 accggtcgca tcgatcgcgc tcaccgccag tgtcgccttg tgggtgtcga tcccgatcgt
     1621 caccatcgcc tgtcctccct cgcgtgggta gactcggatg cgcgcgaccg ggcggacagg
     1681 catgcgtgcg gctcgccgaa agagatcacg ctcctgactt gtttagatgc acccagcacc
     1741 tgggcctcgc gggccgtacg gtgc
//
LOCUS       DATAIX010000494         4202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_279897, whole genome shotgun sequence.
ACCESSION   DATAIX010000494 DATAIX010000000
VERSION     DATAIX010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279897"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1723
                     /locus_tag="VHM65_07415"
     CDS             <1..1723
                     /locus_tag="VHM65_07415"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX2705571.1"
                     /translation="RGIDWQPVAQTKHIFVTGGVASSLGKGLTASSLGHLLRSRGLRV
                     TMQKLDPYLNVDPGTMNPFQHGEVFVTDDGAETDLDIGHYERFLDVSLLGSANVTTGQ
                     IYSRVIAQERRGEFLGDTVQVIPHITNEIKSRMRSEADHPYGGGPGPDVIITEIGGTV
                     GDIESLPFLEAARQVRHDLGRDQVFFLHVSLVPYLAPSGELKTKPTQHSVAALRQVGI
                     QPDAIVCRADREIPDSVKRKISLMCDVDTEAVVAAVDAQSIYDIPRVLHAEGLDAYVI
                     RRLGLNFRDVNWAAWDRMLKRVHDPEHQVEIALVGKYIDLPDAYLSVTEAMRAGGFHH
                     DARVVIRWVASDECATPAGAAKALGGVDAILVPGGFGVRGIEGKLGALHWARERQVPT
                     LGICLGLQCMVIEYARSVLGITDASSSEFDPGTTHPVIATMQEQKAFVEGAGDLGGTM
                     RLGTYPAVLRDGSVVAVAYGRTAVDERHRHRYEVNNAYRAQLEEAGLVFSGTSPDGAL
                     VEFVELPADVHPYYVGTQAHPEFLSRPHRAHPLFAGLVGAALARHRAERLVEVEREHA
                     VSVGTSA"
     gene            1720..2388
                     /locus_tag="VHM65_07420"
     CDS             1720..2388
                     /locus_tag="VHM65_07420"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX2705572.1"
                     /translation="MSLGAPVRRGATLQDEISPRPVLSTRLSHAGMITSMRVDEVDLG
                     AGGVVQREYVEHPGAVLVVALRAIGGVDHVALIRQYRHPVGAYLWELPAGLLDVVGED
                     PWLAAERELAEEVSLRAERWDVLIDAFASPGGFGEAIRIFLARDLSDTLPPPGFVPHA
                     EEVDLETAWVPLAEAYTAVLQGRLHSPGSVIGLMAAYGARAAGWETLRPRDAPWPEHP
                     AYRA"
     gene            2417..2917
                     /locus_tag="VHM65_07425"
     CDS             2417..2917
                     /locus_tag="VHM65_07425"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX2705573.1"
                     /translation="MSPTDRPDVWDERTTLLTMWRYTRAVAAAKCSDAPATAIGAPTV
                     PTSPLTTLGGIANHQRWVEHWWLDVVFLGHEDRAPWTDADPDAEFRIGAEQPISLTLR
                     EWADQSAAIEAAIEGHDLDERGRDWRRSTEPPTLRWVLLHLIEENARHNGHLDLLREI
                     ADGATG"
     gene            2980..>4202
                     /gene="aspS"
                     /locus_tag="VHM65_07430"
     CDS             2980..>4202
                     /gene="aspS"
                     /locus_tag="VHM65_07430"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX2705574.1"
                     /translation="MLRTHEAGTLRASHAGSTVTLTGWVARRRDHGGVAFLDVRDASG
                     VSQVVVRDELLAGEDARDLRSEYVVRVTGEVRSRPEGNANPDLPSGEVEVVASALEVL
                     NPSAPLPFQIDERVTVGEEVRLRHRYLDLRRPGATSAGTAIRLRSKVNQAARRVLERH
                     DFVEIETPTLTRSTPEGARDFLVPARLAPGRWYALPQSPQLFKQLLMVAGMERYYQMA
                     RCYRDEDFRADRQPEFTQLDIEMSFVDQDDIMALGEEIAAELWGVIGVELRGPFPRMT
                     YAEAMARFGTDKPDLRFGLELSDCTAFFAGTTFRVFRQEYVGALVMPGGASQPRKALD
                     ASQEWARQRGARGLAYVLVGEDGELGGPVAKNLTDTERAGLAAHVGASGGDCIFFAAG
                     ATKASRALLGAARLEV"
BASE COUNT          594 a         1537 c         1464 g          607 t
ORIGIN      
        1 ccgtggcata gactggcaac ccgtggcgca gaccaagcac atctttgtga ccggaggcgt
       61 ggcctcgtcg ctcggcaagg gcctgaccgc ctcaagcctc ggacatctcc ttcgctcccg
      121 gggcctgcgc gtcacgatgc agaagctcga cccttacctc aacgtcgacc ccggcacgat
      181 gaacccgttc cagcacgggg aggtcttcgt taccgacgac ggggccgaga ccgacctcga
      241 catcgggcac tacgagcgct tcctggacgt ctccctgctc gggtcggcca acgtcacgac
      301 gggccagatc tacagccggg tcatcgccca ggagcgccgc ggggagttcc tcggcgacac
      361 cgtgcaggtc atcccgcata tcaccaacga gatcaagtcg cggatgcgct cggaggccga
      421 ccacccgtat ggcgggggcc ccggtcccga cgtcatcatc accgagatcg gtgggacggt
      481 gggtgacatc gagtcccttc ccttcctcga ggcggcccgt caggtgcgcc acgacctcgg
      541 ccgcgaccag gtgttcttcc tccacgtctc gctcgtgccc tacctcgcgc cgagcggcga
      601 gctcaagacc aagccgaccc agcactcggt cgcggcgctg cgtcaggtcg gcatccagcc
      661 cgacgcgatc gtctgccgcg ccgaccggga gatcccggac agcgtcaagc gaaagatctc
      721 gctcatgtgc gacgtcgaca ccgaggcggt cgtcgcggcc gtcgatgcgc agagcatcta
      781 cgacatcccc agggtgctgc atgccgaagg cctcgacgcg tacgtcatcc gtcggctcgg
      841 cctgaacttc cgtgacgtga actgggcggc atgggaccgg atgctcaagc gggtccacga
      901 ccccgagcac caggtggaga tcgccctcgt cggcaagtac atcgacctgc ccgacgccta
      961 tctgtcggtc accgaggcga tgcgagccgg cggcttccac cacgacgccc gggtggtcat
     1021 ccgctgggtc gccagtgacg agtgcgccac cccggcgggc gcggccaagg ccctcggcgg
     1081 cgtcgacgcc atcctcgtcc cgggcggctt cggggtgcgc ggcatcgagg gcaagctcgg
     1141 cgctctgcac tgggcgcgcg agcgccaggt tccgaccctg gggatctgcc tcggcctgca
     1201 gtgcatggtc atcgagtacg cccggtcggt cctcgggatc accgacgcgt cgtcctcgga
     1261 gttcgacccc ggcaccacac acccggtcat cgcgacgatg caggagcaga aggccttcgt
     1321 cgagggtgcg ggggacctcg gcgggacgat gcggctgggg acctacccgg ccgtgcttcg
     1381 cgacgggtcg gtcgtcgccg tggcctatgg gcgcaccgcc gttgacgagc ggcaccggca
     1441 ccgctacgag gtcaacaacg cctaccgcgc gcagctcgag gaggccgggc tcgtcttctc
     1501 cggcacgtcg ccggacggcg cgctcgtcga gttcgtcgag ctcccggccg acgtgcaccc
     1561 gtactacgtc gggacgcagg cacaccccga gttcctctcc cgaccgcatc gggcacaccc
     1621 gctcttcgcc ggcctcgtcg gtgccgccct ggcacgtcac cgtgccgagc gcctcgtcga
     1681 ggtcgagcgc gagcacgccg tctcggtggg gacgtcggcg tgagcctggg ggcaccggtc
     1741 cgcaggggcg cgacgctgca ggacgagatc agtccccggc cggtcctgtc cacccggctg
     1801 agccacgccg ggatgatcac ctccatgcgg gtcgacgagg tcgacctcgg tgcggggggc
     1861 gtggtccagc gcgagtatgt cgagcacccc ggcgcggtgc tggtcgtggc ccttcgggcg
     1921 atcggcggag tcgaccacgt cgccctgatc cgccagtacc gccacccggt cggtgcctac
     1981 ctgtgggagc tgcccgccgg cctgctcgac gtcgtcggcg aggacccctg gctcgccgcc
     2041 gagcgtgagc tcgccgaaga ggtctccctg cgggccgaac gctgggacgt gctcatcgac
     2101 gccttcgcct ccccgggcgg gttcggcgag gccatccgga tcttcctcgc ccgcgacctg
     2161 tccgacacgc tccctccgcc ggggttcgtg ccgcacgccg aggaggtcga cctcgagacg
     2221 gcctgggtgc ccctggccga ggcgtacacg gccgtcctcc agggccgcct gcacagcccg
     2281 ggctcggtca tcgggctcat ggccgcgtac ggcgcgcggg ccgccggctg ggagacgctg
     2341 cgcccgcggg acgcgccgtg gcccgagcac ccggcatacc gcgcctgacg ggcctggccg
     2401 tgccactgtt ggtggcatga gcccgactga ccgaccggac gtgtgggacg agcgcacgac
     2461 cctgctgacc atgtggcgct acacgcgggc ggtcgcggcg gccaagtgca gcgacgcccc
     2521 cgccacagcg atcggtgcgc ccaccgtacc gacctcaccc ttgacgacgc tcgggggtat
     2581 cgccaaccac cagcgctggg tcgagcactg gtggctcgat gtggtgttcc tcggccacga
     2641 ggaccgtgcg ccgtggacgg acgccgaccc ggacgccgag ttccggatcg gcgccgagca
     2701 gccgatctcg ctgaccctgc gcgagtgggc cgaccagtcg gccgcgatcg aggccgcgat
     2761 cgagggccac gacctcgacg agcggggacg tgactggcgg cgcagcaccg agcccccgac
     2821 gttgcgctgg gtgctgctgc acctcatcga ggagaacgct cggcacaacg gccacctcga
     2881 cctgctccgc gagatcgcgg acggggcgac cggatgagcc cgggggcgcc ctttccgtag
     2941 gatgggcggg ttaccctcac ctccccgaag gatccgctcg tgctgcgcac ccatgaggct
     3001 ggcaccctgc gtgcctccca cgccggatcg accgtcacgc tcaccggctg ggtggcccgc
     3061 cggcgcgatc acggcggcgt cgcgttcctc gacgtgcgcg atgcgtccgg ggtgagccag
     3121 gtcgtcgtcc gcgacgagct cttggccggc gaggacgccc gcgacctgcg cagcgagtat
     3181 gtcgtcaggg tcaccggaga ggtccgcagt cggcccgagg gcaacgcgaa ccccgacctg
     3241 cccagtggcg aggtcgaggt cgtggccagt gcgctcgagg tgctcaaccc cagcgcgccg
     3301 ctgcccttcc agatcgacga gcgggtcacg gttggcgagg aggtccggct caggcaccgc
     3361 tacctcgacc tgcgccgccc tggcgcgacg tccgcgggca ccgccatccg gctgcggtcc
     3421 aaggtcaacc aggcggcgcg gcgggtgctc gagcgccacg acttcgtcga gatcgagacc
     3481 ccgacgctga cccgctcgac gcccgagggc gctcgcgact tcctcgtgcc cgcgcgcctc
     3541 gcgccggggc gctggtacgc cctgcctcag agcccccagc tgttcaagca gctgctcatg
     3601 gtcgccggga tggagcgcta ctaccagatg gcgcgctgct accgcgacga ggacttccgg
     3661 gccgaccgcc agcccgagtt cacccagctc gacatcgaga tgagcttcgt cgaccaggac
     3721 gacatcatgg ccctcggcga ggagatcgcc gccgagctgt ggggggtcat cggggtcgag
     3781 ctgcgcgggc ccttcccgcg gatgacctat gccgaggcga tggcgcgctt cgggaccgac
     3841 aagcccgacc tgcgcttcgg tctcgagctc agcgactgca cggcgttctt cgccggcacg
     3901 acgttccggg tcttccggca ggagtatgtc ggcgcgctcg tcatgcccgg tggtgcgagc
     3961 cagccgcgca aggcgctcga tgcctcgcag gagtgggcca gacagcgagg cgcccggggc
     4021 ttggcctatg tgctcgtcgg cgaggacggc gagctcggcg ggcccgtggc gaagaacctc
     4081 accgacaccg aacgggctgg tctcgcggcc cacgtcggcg cgtccggcgg cgactgcatc
     4141 ttctttgcgg cgggggccac caaggcatcc cgggccctgc tcggggccgc acgactggag
     4201 gt
//
LOCUS       DATAIX010000495         2674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_220264, whole genome shotgun sequence.
ACCESSION   DATAIX010000495 DATAIX010000000
VERSION     DATAIX010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220264"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..327)
                     /locus_tag="VHM65_07435"
     tRNA            complement(254..327)
                     /locus_tag="VHM65_07435"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(293..295),aa:Gly,seq:tcc)
     gene            433..506
                     /locus_tag="VHM65_07440"
     tRNA            433..506
                     /locus_tag="VHM65_07440"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:467..469,aa:Pro,seq:tgg)
     gene            746..2083
                     /gene="tig"
                     /locus_tag="VHM65_07445"
     CDS             746..2083
                     /gene="tig"
                     /locus_tag="VHM65_07445"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX2705575.1"
                     /translation="MKSALETLNPTRVKMTVEVPFSELKPSLDAAYKTIGSQIQVPGF
                     RRGKVPARIIDQRVGRGAVMQEAVNEALPKLYGQAIEDNSVRPIGRPDVDVTEIPVED
                     GQELRFTVEVDVRPTLELPDFDGIAVEVDPAAASESDIEERLTELRERFGTLTTVERA
                     AAQGDFVSIDLSASIDGEEIDTVSGVSYEVGSKNMLDGLDEAVVGMSAGQTTSFLAPL
                     AGGEHEGADAECEVTVQSVKERVLPEVGDEFAQMASQFDTLDELRADLAAQAERAAKF
                     AQGVQARDRILEHLLDTIEIPVPDAIVVAEVTSHLEGEDRLADDEHRAEVDANTRKAL
                     RSQFLLDAIVEKHEIGVEQNELIEYIVMSAQQYGMDPNTFMQAIDREGQIPSIVAEVS
                     RRKALASVLEKATVTDTTGATVDLEAVQRPEAPAAQGPDAPAVQRPEAPAAEG"
     gene            2122..>2674
                     /locus_tag="VHM65_07450"
     CDS             2122..>2674
                     /locus_tag="VHM65_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705576.1"
                     /translation="MRATATRNGLRVHAIAGTHTVLLGLDLADPAGCLGFAIHRTDHT
                     EDEAYWLRGMKTFASLVPHPPPGTDYSTAEHPVQGFQWGDYTAKPEHDYTYRVSAMGG
                     APGALVPTRTVSVRVRTEATDDGVHGVHFNRGVAGSQAFARAFPNGVPADPDESSPAM
                     AWLSRGLGEAFRSFVAEAAGDGWG"
BASE COUNT          416 a         1012 c          917 g          329 t
ORIGIN      
        1 cgccggcact tcgcctccgc ccaggatcgc aacgccgccg tcggcgccca ctggtatgcc
       61 gaccaggcgc gggacgagct cgggcggctc gccctcgccc tggcgaccgc gacgcggcac
      121 gccggcactt cgcctccgcc caggatcgca acgccgccgt cggcgcccac tggtatgccg
      181 accaggcgcg ggacgagctc gagcggcacg acctatcgca gtagcgcccg cccgctggcc
      241 tggggtgatg cggtggagcg ggtgaccggg atcgaaccgg catggccagc ttggaaggct
      301 ggggctctgc cattgagcta cacccgcggg gcgccgattc gggaccggcg ctctcggggc
      361 gttagcctaa tgcccgcgtg cggccccgcc gaaccgcggc cgccaccggc cgccgacgac
      421 tggccaccgg cgcggggtat ggcgcagctt ggtagcgcgt ccgctttggg agcggaaggc
      481 cgtcggttcg aatccggcta ccccgacaca ccgctacccc gacacaccgc tacctcgaca
      541 caccgctacc ttgacgcacg gcttcctcga cacatccaga ccccgacgcc ggtatggcgc
      601 gacgcacccg agcgtccgcg atcgtcgcgg gcggcgcccc accgggtcgt cgccacacgg
      661 tagggtggtg ggttggtgcg tccagctggc gcagacgagg cgaagcagca ccccgaccaa
      721 cacacgacac cctggagtac ccgcagtgaa gagcgcgctc gagaccctca acccgacccg
      781 ggtcaagatg accgtcgagg tccccttctc cgagctcaag ccgagcctcg acgcggcata
      841 caagacgatc ggctcgcaga tccaggtgcc cggcttccgc cggggcaagg tcccggcccg
      901 catcatcgac cagcgcgtcg gtcgcggtgc ggtcatgcag gaggccgtca acgaggcgct
      961 gccgaagctc tacggccagg cgatcgagga caacagcgtc cgcccgatcg gccggcccga
     1021 cgtcgacgtc accgagatcc cggtcgagga cggccaggag ctcaggttca ccgtcgaggt
     1081 cgacgtgcgc ccgaccctcg agctgcccga cttcgacggc atcgcggtcg aggtcgaccc
     1141 ggcggcggcg agcgagagcg acatcgagga gcggctcacc gagctgcgcg agcgcttcgg
     1201 cacgctgacg acggtcgagc gggccgccgc gcagggcgac ttcgtctcga tcgacctgtc
     1261 ggcctcgatc gacggcgagg agatcgacac cgtctcgggc gtctcctacg aggtcggcag
     1321 caagaacatg ctcgacggcc tcgacgaggc cgtcgtcggc atgagcgccg gccagaccac
     1381 gagcttcctg gcgcccctgg ccgggggcga gcacgaaggc gccgacgccg agtgcgaggt
     1441 gaccgtccag tcggtcaagg agcgtgtgct gcccgaggtc ggcgacgagt tcgcccagat
     1501 ggcctcgcag ttcgacaccc tcgacgagct cagggccgac ctggccgccc aggccgagcg
     1561 cgccgcgaag ttcgcccagg gggtccaggc ccgcgaccgg atcctcgagc acctcctgga
     1621 caccatcgag atcccggtgc ccgacgccat cgtcgtcgcc gaggtcacct cgcacctcga
     1681 gggtgaggac cgcctcgcgg acgacgagca ccgcgccgag gtcgacgcca acacccgcaa
     1741 ggcgctgcgc tcgcagttcc tcctcgacgc gatcgtcgag aagcacgaga tcggcgtcga
     1801 gcagaacgag ctgatcgagt acatcgtcat gtcggcgcag cagtacggca tggaccccaa
     1861 tacgttcatg caggccatcg accgcgaggg ccagatcccc tcgatcgtcg ccgaggtctc
     1921 ccgccgcaag gccctggcct cggtcctgga gaaggccacc gtcaccgaca cgaccggcgc
     1981 cacggtcgac ctcgaggccg tgcaacgccc ggaggccccc gccgcgcagg gcccggacgc
     2041 cccggccgtt cagcgcccgg aggcccccgc cgcggagggc tgaccgcgcg gtagggcggg
     2101 caccccgcct accctgtgga catgcgcgcc actgcgacgc ggaacggcct gcgggtccac
     2161 gccattgcgg gcacccacac ggtgctgctc gggctcgacc tggccgaccc cgccggctgc
     2221 ctcggcttcg ccatccaccg cacggaccac accgaggacg aggcctactg gctgcggggg
     2281 atgaagacct tcgcctcgct cgtgccgcac ccgccgcccg ggaccgacta ctcgaccgcc
     2341 gagcacccgg tccagggctt ccagtggggc gactacaccg ccaagccgga gcacgactac
     2401 acctaccggg tcagcgcgat gggcggggcg ccgggtgcgc tcgtcccgac ccggacggtg
     2461 tccgttcgcg tccggaccga ggcgaccgac gacggcgtcc acggcgtcca cttcaaccgt
     2521 ggcgtggccg gcagccaggc cttcgcgcgg gccttcccca acggcgtgcc cgccgacccg
     2581 gacgagtcga gcccggccat ggcgtggctc tcccgcggtc tcggggaggc ctttcgttcg
     2641 ttcgtcgccg aagccgccgg ggatggctgg gggc
//
LOCUS       DATAIX010000496         2877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_190442, whole genome shotgun sequence.
ACCESSION   DATAIX010000496 DATAIX010000000
VERSION     DATAIX010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190442"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VHM65_07455"
     CDS             <1..579
                     /locus_tag="VHM65_07455"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase catalytic subunit"
                     /protein_id="HEX2705577.1"
                     /translation="VVGVEVNISCPNVASRGLVFACDPLALAKVVSLVRAEVSDAIPV
                     FAKLSPDVTDIVSIALACAKGGATGLTMINTLLGMVIDTDRMRPHLGGVTGGLSGPAI
                     RPIAVRAVWQVSAAMRAGHLPALPVIGVGGVRTGADALELVAAGASAVQVGTAVFNDP
                     SAPVRVLRELEALLDERGFRSFGEVVGLAHGR"
     gene            590..1420
                     /gene="pyrF"
                     /locus_tag="VHM65_07460"
     CDS             590..1420
                     /gene="pyrF"
                     /locus_tag="VHM65_07460"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX2705578.1"
                     /translation="MSTPFGVRLRAAMDAYGALCVGIDPHDGLLDGWGLPHDVSGLAR
                     FSAVCVEALGGQVACVKPQSAFFERFGSAGVAVLERTLADLRAAGTLTILDVKRGDIG
                     STMAAYAVAYLGEASPLRADAITVSPYLGYGSLRPALDLAAETGRGVFVLALTSNPEG
                     ASVQHAVRGGRSVAGAVVDGVRADNAAARGVGRLGSVGLVVGATVGSAVTDLGLDLAA
                     ANAPLLAPGVGAQGAAAGDLEAVFGAALPNVLASASREVLGAGPSVAALRAAAARCTL
                     "
     gene            1503..1829
                     /gene="mihF"
                     /locus_tag="VHM65_07465"
     CDS             1503..1829
                     /gene="mihF"
                     /locus_tag="VHM65_07465"
                     /inference="COORDINATES: protein motif:HMM:NF041260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HEX2705579.1"
                     /translation="MALSPLPPLTPEQRAAALAKAAAARRERAAVKNRLKYAQGSLAA
                     VIAQGKSNEVVGKMKVSAMLESMPGVGRVRARQIMIEVGIAETRRVRGLGVNQTAALV
                     QRFDHA"
     gene            1826..2404
                     /gene="gmk"
                     /locus_tag="VHM65_07470"
     CDS             1826..2404
                     /gene="gmk"
                     /locus_tag="VHM65_07470"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX2705580.1"
                     /translation="MTHGGRLIVLAGPTAVGKGTIASYIRTHFPQVYLSVSMTTRAPR
                     PGEVDGVHYLFVADEEFDRLVAAGQFLEYALVHGAAKYGTPRGPVERALASGRDALLE
                     IDLQGARQVKATMPDALFVFLQPPSWGELVRRLEGRGTESEGEREVRLRTARDEMAAR
                     EEFDVVIVNDDVSRASEALVALFRTAAVPGRG"
     gene            2452..2715
                     /gene="rpoZ"
                     /locus_tag="VHM65_07475"
     CDS             2452..2715
                     /gene="rpoZ"
                     /locus_tag="VHM65_07475"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX2705581.1"
                     /translation="MSGTVANPIGITKPPIDDLLAIADSKYALVIYSAKRARQINAYY
                     SQLQEGLLDYVGPLVETEVSEKPLSIALREISQGLLTSEPTEG"
BASE COUNT          379 a         1031 c         1046 g          421 t
ORIGIN      
        1 gtggtggggg tcgaggtgaa catctcctgc cccaacgtcg ccagccgggg gctcgtcttc
       61 gcctgcgacc cgctcgcctt ggccaaggtc gtctcgctcg tccgggccga ggtgagtgac
      121 gccataccgg tgttcgccaa gctgagcccg gacgtcaccg acatcgtcag catcgcgctg
      181 gcctgcgcca agggcggcgc gaccgggctc accatgatca acaccctgct cggcatggtc
      241 atcgacaccg accgcatgcg cccgcacctc ggcggcgtga ccggtggcct gtccgggccc
      301 gcgatccgcc cgatcgccgt ccgggcggtc tggcaggtga gcgccgcgat gcgggccggc
      361 cacctgccgg cccttccggt catcggggtc ggcggcgtcc ggaccggtgc cgacgcgctc
      421 gagctcgtgg cggcgggggc gagcgcggtc caggtcggga cggccgtctt caacgacccc
      481 tcggcgcccg tgcgcgtcct gcgcgagctc gaagcgctcc tcgacgagcg gggtttcagg
      541 agcttcggtg aggtcgtcgg cctggcccac ggacggtgag cacctcgaca tgagcacgcc
      601 cttcggagtg cggctgcgag cggccatgga cgcgtacggc gcgctgtgcg tcgggatcga
      661 cccgcacgac gggctgctcg acggctgggg gctgccccac gacgtctcgg gcctcgcgcg
      721 gttctcggcg gtgtgtgtcg aagccctcgg ggggcaggtc gcctgcgtca agccgcagtc
      781 ggcgttcttc gagcggttcg ggtcggcggg cgtcgccgtc ctcgagcgca ccctcgcgga
      841 cctccgcgcg gccggcaccc tgacgatcct cgacgtcaag cgcggggaca tcggctcgac
      901 gatggccgcc tatgccgtgg cctacctcgg cgaggcgtca cctcttcggg ccgacgccat
      961 cacggtgagc ccctacctcg gctacgggtc gctgcgcccg gccctcgacc tcgcggccga
     1021 gacgggccgc ggcgtgttcg tcctggcact gacgtccaac cccgagggcg cctcggtcca
     1081 gcacgccgtg cgagggggcc gcagcgttgc cggggcagtc gtcgacgggg tcagggccga
     1141 caacgccgcg gccaggggcg tggggaggct cggcagcgtc ggcctggtcg tcggcgccac
     1201 ggtggggtct gccgtgaccg acctgggact ggatcttgcg gccgcgaacg cgcccctgct
     1261 cgcgcccggg gtgggtgctc agggtgccgc ggcgggcgac ctcgaggcgg tcttcggcgc
     1321 ggcgctgccc aacgtcctgg caagcgcgag ccgggaggta ctgggcgccg ggccgagcgt
     1381 ggcggccctg cgtgcggccg ccgcccgatg cacgctctga cttcggaccc gcgtcgttgc
     1441 gcgggcccga aggggtcgat aggttgcagg tcaggccccc agcactgtga ggagtacgca
     1501 ctgtggcgct ttccccgctt cccccgctga cgccggagca gcgcgctgcc gcgctggcga
     1561 aggccgccgc ggcccgacgc gagcgcgctg ccgtgaagaa ccggctcaag tacgcccagg
     1621 gctcccttgc cgccgtcatc gcccaaggga agagcaacga ggtcgtcgga aagatgaagg
     1681 tctcggcgat gctcgagtcg atgcccggcg tcggccgggt gcgggcccgc cagatcatga
     1741 tcgaggtcgg gatcgccgag acccgccggg tccgggggct cggcgtcaac cagactgcgg
     1801 ctctcgtcca gcgcttcgac cacgcgtgac ccacggcggt cgcctcatcg tcctggccgg
     1861 cccgacggcc gtcgggaagg ggacgatcgc gtcatacatc cgcacacact tcccccaggt
     1921 ctacctgtcc gtgtcgatga cgacccgcgc gccgcgtccc ggtgaggtcg acggcgtcca
     1981 ctacctgttc gtcgccgacg aggagttcga ccggctcgtc gcggccggtc agttcctgga
     2041 gtatgcgctc gtccacggcg cggcgaagta cggcaccccg cggggcccgg tggagcgggc
     2101 gctggcctcg ggccgcgatg cgctgctcga gatcgacctg cagggagcac gccaggtcaa
     2161 ggcgacgatg cccgatgcgc tcttcgtgtt cctgcagccg ccgagctggg gcgagctcgt
     2221 gcgccgtctc gagggccggg gcaccgagag cgagggggag cgggaggtgc ggctgcgcac
     2281 cgcccgggac gagatggccg cccgcgagga gttcgacgtc gtcatcgtca acgacgacgt
     2341 gtcgcgcgcc agcgaggcgc tcgtcgccct cttccgcacg gccgccgtgc cgggcagggg
     2401 atagaatggg ccgttgcccc gacagaccgc cgaacgaaac gagactcacc cgtgtccgga
     2461 accgtggcca accccatcgg catcaccaag ccgccgatcg acgacctgct cgccatcgcc
     2521 gactcgaagt acgccctggt catctactcg gccaagcgcg cccggcagat caacgcctac
     2581 tactcccagc tgcaggaggg cctgctcgac tacgtcggcc cgctcgtcga gaccgaggtc
     2641 tcggagaagc cgctgtcgat cgccctgcgt gagatcagcc agggtctgct cacgtcggag
     2701 ccgacggagg gctgagccct tctgtcacgg gtgatgaagg tcgtcctcgg cgtcagcggc
     2761 gggatcgcgg catacaaggc gtgctcgctc ctgcgtctgt tcaccgagaa cggccatgac
     2821 gatgacatcg gcggatgccg cccgctcggt cacggccgcc tccatctgca gcgccgt
//
LOCUS       DATAIX010000497         1885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_86952, whole genome shotgun sequence.
ACCESSION   DATAIX010000497 DATAIX010000000
VERSION     DATAIX010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86952"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VHM65_07480"
     CDS             complement(<1..251)
                     /locus_tag="VHM65_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705582.1"
                     /translation="MAVAFRNVELGEGTPLEQWPYEALVTVIERGTVGDWARLTRAIR
                     REPWGQVARQAEEYLGQAQPWGVGPLLQRAVDAARAEAER"
     gene            complement(251..976)
                     /locus_tag="VHM65_07485"
     CDS             complement(251..976)
                     /locus_tag="VHM65_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705583.1"
                     /translation="MASRFAASPLRSAALAEVLASAARLQRAVPDAILVGDAAAALYA
                     AHRDSFVHDHVLVDLQDRYLEVLEAVEATEGWVTSVRASAPPQTIMGSLGGVEAGLRQ
                     LRRSRPLEVEEVDLGEAGVVRVPTVEEILRVKSYLVVLRNQVRDYLDVVAVAETTGLE
                     RAVSTLVGIAAYYVDRSREEDSVLTALVQRLSEPNPRDHRVTRQLAAYKGLDPRWHRW
                     STVVAACQALADAIVRVVEERVP"
     gene            1176..>1885
                     /locus_tag="VHM65_07490"
     CDS             1176..>1885
                     /locus_tag="VHM65_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705584.1"
                     /translation="MDEYELLIRHTEAWAAAKTRPLDTDLLSSLLHLRAVHDRRPGTS
                     WPAGSAEHLLTVRLPGHGPLGAADPEAIVATLDTFWRFLRGTGRMASGSAEPRDLLRE
                     ARRAIPTMQARAEDPAYFGQSKALQQFGAQIGITLDDLADMDDLNARMQQITDAWNAL
                     PEEERFARMPRPGSAGSLAADELAQALGEYTSNGEPSGLGPDADDVIPVQDAAVVARQ
                     ARESGFLRSCLALAEWVG"
BASE COUNT          293 a          677 c          646 g          269 t
ORIGIN      
        1 cgctcggcct cagcccgggc cgcatcgaca gcgcgctgca acaggggacc cacgccccac
       61 ggctgggcct ggccgaggta ctcctcggcc tgccgagcaa cctgccccca aggctcacgg
      121 cggatcgcgc gggtgagtcg ggcccagtca ccgaccgtgc cacgctcgat gaccgtcacg
      181 agcgcctcgt agggccattg ttcgagggga gtcccttctc ccagctccac gtttcggaac
      241 gcgacggcca tcacggcact cgctcttcga ccactcgcac gatcgcgtcg gcgagggcct
      301 ggcaggccgc gacgaccgtc gaccagcgat gccagcgcgg gtcgaggccc ttgtaggcgg
      361 ccagctgccg ggtcacccgg tggtcgcgcg ggttgggctc agagagccgc tgcaccaggg
      421 cggtgagcac ggagtcctcc tctcgggacc ggtccacgta gtaggcggcg atgccgacca
      481 gcgtggacac agcccgctcg aggcccgtcg tctcggcaac ggccaccacg tcgaggtagt
      541 cgcgcacctg gttacgcagc acgacgagat agctcttgac ccgcaggatc tcctcgaccg
      601 tgggcacccg caccactccc gcctcgccga gatcgacctc ctccacctcc aggggccgcg
      661 agcgccgcag ctgacgcagc cctgcctcca caccgcccag gctgcccatg atggtctgcg
      721 gtggggcact cgcgcgcacg ctggtgaccc agccctcggt ggcttccacg gcctccagaa
      781 cctcgaggta gcgatcctgg aggtcgacca gaacgtggtc gtgaacgaag gagtcgcggt
      841 gagcggcata cagcgcggcc gctgcatcgc cgaccaggat ggcgtccggc acggcacgct
      901 gcagtcgcgc cgctgaggcc agcacctcgg ccagagcggc gctgcggaga ggagatgcgg
      961 cgaagcgaga agccatgcat ggttggtatc agatgtgata accgtcaatc cgaggaggcc
     1021 gcctcgtccg ctgctgtcac cctgcatcca ctgctgccgg cgcgatctcg gcgtcctcga
     1081 catccaggcc acgcgtggga accccccccg ctttgcgagg accgtgctcg gtgtccttgt
     1141 ggcatcttcg gacgaccaag tgccaagatt ctcgggtgga cgagtacgag ctgctgatcc
     1201 gccataccga ggcctgggcg gccgccaaga cgcggccgct ggacaccgac ctactgagca
     1261 gtcttcttca tctgcgcgcc gtccatgacc gccggccagg cacgtcgtgg ccggcgggaa
     1321 gcgccgagca cctgctgacg gtgcggttgc ctggccacgg cccgctcggc gctgccgacc
     1381 ccgaggcgat cgtggcgacg ctggacacgt tctggcggtt cctgcgcggg accggccgga
     1441 tggcgtccgg ttcagcggag cccagggacc tgctgcgcga ggcgcgccgc gccataccga
     1501 ccatgcaggc tcgcgccgag gacccggcct acttcgggca gtccaaggcg ctgcagcagt
     1561 tcggcgcgca gatcgggatc accctcgacg accttgccga catggacgac ctcaacgccc
     1621 ggatgcagca gatcaccgac gcatggaacg cgctccccga ggaggaaagg ttcgcccgga
     1681 tgccgcgccc cggttcggcc gggagcctgg ctgccgatga gctggcccag gccctcggcg
     1741 agtacaccag caatggggaa ccgagcggcc tcggaccgga tgccgacgac gtcatcccgg
     1801 ttcaggatgc tgcggtcgtg gcccggcagg cgcgggagtc ggggttcctg cggtcctgcc
     1861 tggcgctggc cgagtgggtc ggcga
//
LOCUS       DATAIX010000498         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_190471, whole genome shotgun sequence.
ACCESSION   DATAIX010000498 DATAIX010000000
VERSION     DATAIX010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190471"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VHM65_07495"
     CDS             <1..563
                     /locus_tag="VHM65_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705585.1"
                     /translation="VETGVGLIPAGGGSTAMARRAAERAAGAPVDGFAFFRAALETIA
                     LSRVSTSAEEARALGLLGPADLVTADPERQWTDAASVARTLAEVGYRPPAERPIAVLG
                     RRGIAAAEALTYSQRAGRYLSEHDRTVVLELARVMSGGDVAEGTELPEAYLLDLEREA
                     FLRLLGERLTRDRIRHTLKTGKPLRN"
     gene            581..>1572
                     /locus_tag="VHM65_07500"
     CDS             581..>1572
                     /locus_tag="VHM65_07500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX2705586.1"
                     /translation="MRDAVIVSAVRTAVGRAPRGALRETPPEELAMAAVREALVRAPG
                     LDPAEVEDVILGCAMPEGAQGLNIARYVALGAGLPVSTSAMTVNRFCASGLQALALAS
                     QSVATGVADVVVAGGLESMSLVPMTGWHFAPNPGLAEHWPQVFLNMGLTAEEVASRED
                     VSRDDQDAWGLRSHRRAVAAIDAGRFRDETVPITAGRTVVKDGRPRVESAIFETDEGP
                     RRDTDAARMASLPAVFAVDGTVTAGNSSQASDGAAAMVVMTGARAAALGLTPLGRLVS
                     YATAGVAPEVMGLGPVEAVPKALLKAGLSLDDLAVIELNEAFAAQVVAVVRRLGL"
BASE COUNT          209 a          571 c          573 g          219 t
ORIGIN      
        1 tggtcgagac cggtgtgggt ctgattcccg ccggcggcgg ctcgaccgcg atggcccgcc
       61 gtgccgcgga gcgggccgcc ggtgcccccg tcgacggttt tgccttcttc cgcgccgcac
      121 ttgagacaat cgcgctgtcc cgggtttcca cgagcgccga ggaggcccgc gctctgggcc
      181 tgctcggccc ggccgacctc gtgacagccg accccgagcg gcagtggacc gacgcggcgt
      241 cggtcgccag gacactcgcc gaggtgggct accgaccacc ggccgaacga ccgatcgccg
      301 tcctcgggcg gcgtgggatc gccgctgctg aagccctgac ctacagccag cgggccggcc
      361 gctatctgag cgagcacgat cgaacggtcg tccttgagct ggcgcgggtg atgagcggcg
      421 gcgacgtcgc cgaggggacc gagctgcccg aggcgtatct gctcgacctc gagcgtgagg
      481 ccttcctgcg gctgctcggc gagcgactga cgcgcgaccg gatccgccac accctcaaga
      541 ccggcaagcc gctgcgcaac tgacctgcta ggagcgaacc atgcgagacg ccgtcatcgt
      601 gagcgccgtc cggacggccg tcggccgggc gccgcggggc gcactgcgcg aaacgccccc
      661 cgaggagctg gccatggcgg cagtgcgtga ggccctcgtc agggcgcccg gtctggaccc
      721 ggccgaggtg gaggacgtaa tcctcggttg cgcgatgccc gagggcgccc agggcctcaa
      781 cattgcccgc tacgtcgccc tgggcgccgg gctacccgtc tcgacgagcg cgatgacggt
      841 caaccggttc tgcgcgtcgg gcctccaggc cctcgccctc gcctcgcagt cagtggccac
      901 cggcgtcgca gacgtggtcg tcgcgggcgg tctcgaatcg atgagcctgg tgcccatgac
      961 cggctggcac ttcgcgccga accccgggct ggccgagcac tggccgcagg tcttcctgaa
     1021 catgggcctc accgccgagg aggtggccag ccgcgaggat gtcagccgcg acgaccagga
     1081 cgcctggggg ttgcgcagcc accggcgcgc cgtcgctgcg atcgacgccg gccgcttccg
     1141 cgatgagacg gtcccgatca ccgccgggcg caccgtcgtc aaggatggcc gcccgcgggt
     1201 ggagagcgcg atcttcgaga ccgacgaagg gccccgtcgc gacacggacg cggcccggat
     1261 ggcatcgttg ccggcagtct tcgcggtcga cgggacggtc acggccggca acagttccca
     1321 ggccagcgac ggggcggccg cgatggtcgt catgaccgga gcgcgcgctg cggccctggg
     1381 cctcaccccg ctcggtcggc tcgtctcgta cgcgaccgcg ggtgttgcgc ccgaagtgat
     1441 gggtctgggc ccggtcgagg cggtccccaa ggcgctcctg aaggcaggtc tctccctgga
     1501 cgacctcgcc gtcatcgagc tcaacgaggc gttcgccgcc caggtcgtcg ccgtcgtgag
     1561 gcggctcggc ct
//
LOCUS       DATAIX010000499         1529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_116486, whole genome shotgun sequence.
ACCESSION   DATAIX010000499 DATAIX010000000
VERSION     DATAIX010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116486"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VHM65_07505"
     CDS             complement(<1..426)
                     /locus_tag="VHM65_07505"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX2705587.1"
                     /translation="MASGSVASRVLGLVRTLLLTAAIGLSFAGDAFAVANTLPNIIYL
                     VIAGGVLNSVLVPQLVKAASDPDGGQEFTDRIITMAGAAILGLTIVATAAAPLLTRLF
                     AGQFTAETLSLATAFAFICLPQILFYGLYALLGQVLNARG"
     gene            complement(459..>1529)
                     /locus_tag="VHM65_07510"
     CDS             complement(459..>1529)
                     /locus_tag="VHM65_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705588.1"
                     /translation="ILAEKAQWPLFAEPTSGARAGPAAIRTYRLLLGTHLRAQIERVV
                     VLGHPTLSRPVTALISDPGLEILSVPTRAGIATDPGRVATVLDGVPDVEHPDDPGWLR
                     RWTRADALLSAALDAQVDAWGAVLAALGESAWLHPLVLAREVAAAVGPHTTLVVGSSS
                     PVRDLDVMMTPYPPRQRRLVVANRGLAGIDGTVSTALGVALGRREPARQLAVMGDVSF
                     LHDAPGLFLGPDEPRPDLTIVVVNDDGGSIFTLLEQGAPEFAAAFERVFATPHHADLA
                     ALCAASGTSYRLLTTAPELREALHRSPTGTEVIEVRVPRALRRAEAHAMADLARMVID
                     GMPGTTPGTTPGTTPGNAGAVR"
BASE COUNT          225 a          500 c          620 g          184 t
ORIGIN      
        1 ccctctggcg ttgagcacct gcccgagtag ggcgtagagc ccgtagaaga ggatctgcgg
       61 caggcagatg aaggcgaacg ccgtcgccag ggagagggtt tcggcggtga actggccggc
      121 gaacagccgg gtcagcagcg gggcggcagc cgtcgcgacg atggtcaggc cgaggatcgc
      181 ggcgccggcc atcgtgatga tccggtcggt gaactcctgc ccgccgtcgg ggtccgaggc
      241 agccttgacc agctggggga cgagcaccga gttgagcact ccaccggcga tgacgaggta
      301 gatgatgttg ggcagcgtgt tggcgaccgc gaacgcatca ccggcaaagc tgagcccgat
      361 cgcggcggtc agcagcaggg tgcggacgag gccgagcacc cgggacgcga cactcccgct
      421 tgccatggcg atgctagagc gagcgatgct cggcgtgctc atcgcacggc tcctgcgttg
      481 cccggggtgg tgccgggcgt ggtgccgggc gtggtgcccg gcatgccgtc gatgaccatg
      541 cgcgcgaggt cggccatcgc gtgggcctcg gcccggcgca acgcccgcgg gacgcgcacc
      601 tcgatgacct cggtccccgt cggggaacgg tgcagggcct cgcgcagctc gggcgcggtg
      661 gtgagcagcc ggtatgacgt cccgctcgcc gcgcacagcg ccgcaaggtc ggcgtggtgc
      721 ggggtggcga agacccgctc gaaggcggcg gcaaactccg gggccccttg ttcgagcagc
      781 gtgaagatcg acccgccgtc gtcgttgacg acgacgatcg tcaggtcggg gcgcggctcg
      841 tccgggccga ggaagagccc aggcgcgtcg tgcaggaagg acacgtcacc catgaccgcg
      901 agctggcggg ccggctcacg ccgaccgagg gcgaccccga gggccgtcga cacggtgccg
      961 tcgatcccgg ccaggccgcg gttggcgacc acgaggcggc gctgccgcgg tgggtagggg
     1021 gtcatcatga cgtcgagatc gcgcaccggg ctggacgacc cgacgacgag cgtggtgtgc
     1081 ggcccgacag cggcggcgac ctcgcgggcg agcacgagcg gatgcagcca ggcgctctcg
     1141 ccgagggcgg cgagcacggc cccccaggcg tcgacctggg cgtccagcgc ggccgagagg
     1201 agggcatcgg cccgggtcca gcggcgcagc caccccgggt cgtcggggtg ctcgacatcc
     1261 gggaccccgt cgaggacggt cgccacccgg cccgggtcgg tggcgatgcc ggcccgggtg
     1321 gggaccgaga ggatctcgag gccggggtcg ctgatcaggg cggtcaccgg gcgggacagc
     1381 gtgggatgac cgaggacgac gacgcgctcg atctgcgcgc gcaggtgggt cccgagcagc
     1441 agtcggtagg tccggatcgc cgccgggccg gcccgggccc ccgaggtggg ctcggcgaag
     1501 aggggccact gcgccttctc ggcgaggat
//
LOCUS       DATAIX010000500         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_26713, whole genome shotgun sequence.
ACCESSION   DATAIX010000500 DATAIX010000000
VERSION     DATAIX010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26713"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1257
                     /locus_tag="VHM65_07515"
     CDS             73..1257
                     /locus_tag="VHM65_07515"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX2705589.1"
                     /translation="MTIRTPLTLATVHPDSGLRAALGRTPAEVVEEVSRSGIRGRGGA
                     GFRTGLKWRLASEAPAPDGRRHVVCNADEGEPGTFKDRALLSVVPDLVFEGMTIGAWA
                     IGATSGVVYLRGEYVYLREGLEDCLARRREAGLLGTDILDTPDFDFDISIRLGSGAYV
                     CGEETALLESLEGRRGEPRNRLPYPVVRGLDQAPTIVNNVETFAWVPAILARGAAWFV
                     GIGTPGSTGPKLLSVSGDVARPGVYEFPFGVTIRELLEASGGEDAHAVVGGGASGVCV
                     PAADFTRAISYEEVSTGGAIIVLGPGRDLLDVAENFLEFFVDESCGQCTPCRLGNPVL
                     LEGVRSLKRGECTAERLGELQHLGRTMQGTSKCGLGQTSPNFFLSLVDRFGPEILDRV
                     PV"
     gene            1269..>2198
                     /locus_tag="VHM65_07520"
     CDS             1269..>2198
                     /locus_tag="VHM65_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX2705590.1"
                     /translation="MTTSIPRSKEASASVGATVRATINGREVEVASGTTILEAAHACG
                     VRIPTLCHHPDLADVGVCRVCVVEVEGQVNLQPSCVFPITQPLEVSTHSPRVAAVRRD
                     IIDLLLSEHYGDCRTCARNGTCELQDLASEYGVDHYRFGQRTEPVFVRDDTGPSIVFD
                     PDKCILCLRCVRTCAELQGVRALGVSGRGAGVRVTTFADLPLSSEFCINCGQCLNRCP
                     TGALTAVDVTARVRAALEDPRKHVVIQTAPAPRAALGEEFGLPPGTPVTWQLNTALRE
                     LGFDKVFDTTFAADLTIIEEGVELIERLYHALVL"
BASE COUNT          308 a          798 c          772 g          320 t
ORIGIN      
        1 gctggaagga cagcgaagcc tcgcacgcgg gcccaccgac gagccgcggg gcgtcgcgcg
       61 ggaggagtcc cgatgaccat ccggacgcca cttaccctgg caaccgtcca ccccgactcc
      121 gggctgcgcg cggccctcgg gcggaccccc gccgaggtcg tcgaggaggt gtcccgctcc
      181 gggatccggg gccgtggggg agccggcttc cgcaccggac tcaagtggag gctggcgtcc
      241 gaggcgcccg ccccggatgg ccgtcgccac gtcgtgtgca acgcggacga gggggagccg
      301 ggcactttca aggaccgcgc gttgttgagc gtggtgcccg acctcgtctt cgagggcatg
      361 accatcggcg cctgggccat cggggccacc agcggggtcg tctatctgcg gggcgagtac
      421 gtgtacctgc gagaggggct cgaggactgc ctggcccgcc gccgggaggc cggcctgctc
      481 ggcaccgaca tcctggacac cccggacttc gacttcgaca tcagcatccg gctcgggtcg
      541 ggggcctacg tctgcggtga ggagacagcc ctcctcgaga gcctcgaggg acgccgcggc
      601 gaaccccgca accggctccc ctacccggtc gtccggggcc tcgaccaggc gcccacgatc
      661 gtgaacaacg tcgagacgtt cgcgtgggtg ccggcgatcc tggcgcgcgg ggccgcctgg
      721 ttcgtcggca tcgggacgcc ggggtccacg ggtccgaagc tgctgagcgt ctccggcgac
      781 gtcgcgcgtc cgggggtcta cgagttcccc ttcggggtca ccatccgcga gctgctcgag
      841 gcctccggcg gcgaggacgc ccacgccgtc gtcggcggcg gggcgtccgg cgtgtgcgtc
      901 ccggctgcgg acttcacgcg cgcgatcagc tacgaggaag tcagcaccgg gggagcgatc
      961 atcgtgctgg gcccgggccg cgatctgctc gacgtcgccg agaacttcct ggagttcttc
     1021 gtcgacgagt cgtgcgggca gtgcactccc tgcaggctcg gcaatcccgt gctcctggag
     1081 ggtgtccggt cgctgaagcg cggcgagtgc acagccgaac ggctcggcga gctgcagcat
     1141 ctggggcgca ccatgcaagg cacctccaag tgcgggctgg gccagaccag ccccaacttc
     1201 ttcctctcgc tcgtggaccg gttcggtccc gagatcctcg accgagtccc cgtctgaagg
     1261 gagccaccat gaccacgagc atcccccggt cgaaagaggc ctccgcgagc gtcggggcaa
     1321 ccgtccgggc caccatcaac ggccgtgagg tcgaggtcgc cagcggcacg accatcctgg
     1381 aggcggccca cgcctgcggc gtccggatac ccacgctgtg ccaccacccc gacctggccg
     1441 acgtcggcgt gtgccgggtc tgcgtcgtcg aggtcgaagg tcaggtgaac ctgcagccca
     1501 gctgcgtgtt cccgatcacc cagccgctcg aggtcagcac gcatagcccg cgcgtcgctg
     1561 ctgtgcgccg cgacatcatc gacctgctgc tcagcgagca ctatggcgac tgccggacgt
     1621 gcgcccgcaa cggcacctgc gaactccagg acctggcgtc cgagtacggc gtcgaccact
     1681 accgcttcgg ccagaggacc gagccggttt tcgtccgcga cgacaccggc ccgtcgatcg
     1741 tgttcgaccc cgacaagtgc atcctgtgcc tgcgctgcgt ccgcacgtgc gccgagctgc
     1801 agggcgtccg ggcactcggc gtgtcgggcc ggggcgccgg ggtccgggtg acgaccttcg
     1861 ccgaccttcc cctgtcgtcg gagttctgca tcaactgcgg ccagtgcctg aaccgctgcc
     1921 ccacgggggc gctcacggcg gtggacgtca ccgcccgcgt ccgcgccgcg ctcgaggacc
     1981 cgcgcaagca cgtcgtgatc cagaccgcgc ctgcgcctcg ggccgcgctc ggcgaggagt
     2041 tcgggctgcc cccgggcacc ccggtgacct ggcagctcaa caccgcgctg cgcgaactgg
     2101 gcttcgacaa ggtgttcgac acgaccttcg cggccgacct gacgatcatc gaggagggcg
     2161 tcgagctgat cgagcggctg taccacgccc tcgtgctg
//
LOCUS       DATAIX010000501         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_250145, whole genome shotgun sequence.
ACCESSION   DATAIX010000501 DATAIX010000000
VERSION     DATAIX010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250145"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VHM65_07525"
     CDS             complement(<1..849)
                     /locus_tag="VHM65_07525"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2705591.1"
                     /translation="MADFDVVVLGAGPGGYVAAIRCAQLGLKAAVVEERYWGGVCLNV
                     GCIPSKALLKNAELAHVLTHEKAKFGIEGNATMSYGPTHARSRQVSAGIVKGVHFLMK
                     KNKITEINGWGTLTSPTSMTVRGSDGGEQQVTFDNLIIATGSVTRMIPGVSVSANVVT
                     YEEQILDDQLPGSIIIAGSGAIGVEFAYIMRNFGVDVTIVEFLDRMVPTEDADVSKEL
                     LKHYRKLGVKVMLSTKVEAVEDTGSRVRVTVSPAGGGDTQVLEADRLLSAIGFAPRTQ
                     GYGLEAT"
     gene            complement(860..2062)
                     /locus_tag="VHM65_07530"
     CDS             complement(860..2062)
                     /locus_tag="VHM65_07530"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2705592.1"
                     /translation="MGRRVWRVVASHGLAAVGMSLPWPLLMARVWHETGDERLLGLVG
                     AARMLPYVAISWLAGRLADRWARDRVVWWTLGARLVLLAAASQAVATGRTATCVVLLT
                     LTVAAGTPAYPSLAAGLPTLAGRHNDRATDLLVTVEVASFVVGPALGGLLMGAAAAPM
                     VLGVGAALVGAAALLFAGTGMPRPEHVPREPAQGAGRVLRSDAGARGAIALLVVVNAV
                     LAGVGIVLLPHAEAAWPTEPDAYGLVVAGLGFGALAAPVLGRLGQVRSSGTGLAWMGA
                     GLLLVALAPTSGWALAPVGLVSAAAVRLESLATAAIQARVPDAQRASALGIADTAMVA
                     AALAASAVAPLFTLVAPATRVVVGTGVCLSLLPLAARRRRRRSGVTTVAGDAGPARPL
                     DSCGPPYP"
     gene            complement(2105..>2524)
                     /locus_tag="VHM65_07535"
     CDS             complement(2105..>2524)
                     /locus_tag="VHM65_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705593.1"
                     /translation="VLASSPLHRHHAVQVRKVADLIDPIDRALRSTRVLIRRVVVATR
                     SDGPVPASCVAVLTDLATATDILARALGENASPEVGRSELVRVGERTAALVRTSYLST
                     EVLLAQLRSTVIDLLQVTGLSVDEALAVVPPVVDRPT"
BASE COUNT          424 a          892 c          905 g          303 t
ORIGIN      
        1 ggtggcctcc aggccatagc cctgcgtgcg tggcgcgaag ccgatggccg agagcagccg
       61 gtcggcctcg agtacctgcg tgtccccccc accggcgggg gacacggtga cgcgcacacg
      121 ggaaccggtg tcttcgacgg cctcgacctt ggtcgagagc atgaccttga cgccgagctt
      181 ccggtagtgc ttgaggagct ccttggacac gtccgcgtcc tcggtcggga ccatccggtc
      241 gaggaactcg acgatcgtga cgtcgacgcc gaagttgcgc atgatatagg cgaactcgac
      301 accgatcgcg cccgacccgg cgatgatgat cgagcccggc agctggtcgt cgaggatctg
      361 ctcctcgtag gtcaccacgt tggccgacac cgagacgccc gggatcatcc gggtcaccga
      421 gccggtcgcg atgatgaggt tgtcaaaggt gacctgctgc tcaccgccgt cggagccgcg
      481 gaccgtcatc gaggtcggcg aggtcagcgt cccccagccg ttgatctcgg tgatcttgtt
      541 cttcttcatg aggaagtgga cgcccttgac gatgcccgcc gacacctggc gcgagcgcgc
      601 gtgcgtcggg ccataggaca tcgtcgcgtt cccctcgatg ccgaacttcg ccttctcgtg
      661 ggtgagcacg tgggcgagct cggcgttctt gagcagcgcc ttgctcggga tacacccgac
      721 gttgaggcag acaccccccc agtaccgctc ctcgacgacc gcggccttca gcccgagctg
      781 cgcgcagcgg atcgccgcga cataccctcc gggaccggca ccgagcacca ccacatcgaa
      841 gtcagccatg cgccacagcc tatgggtatg gcggtccgca cgagtcgagg gggcgggccg
      901 gtcccgcgtc ccccgccacc gtcgtcacac cggagcgcct gcgacgtcgt cgagcggcca
      961 agggcaacag gctcaggcag accccggtgc caacaacgac tcgggtcgcg ggggcgacca
     1021 gcgtgaacag cggggcgact gcgctggcgg ccagtgccgc ggcaaccatc gccgtgtcgg
     1081 cgatccccag ggcactggcc cgctgggcat ccggcacccg agcctggatc gcggccgtcg
     1141 ccaggctctc gagacgcacc gcagcagcgc tgaccaggcc gaccggcgcc agagcccagc
     1201 cgctggtcgg agcgagcgcg acgagaagca gcccggcgcc catccaggcc agccccgtcc
     1261 ctgaggaacg gacttgcccg aggcggccga ggaccggagc ggcaagcgcc ccgaagccga
     1321 gtccggcaac caccagacca taggcgtccg gctcagtggg ccaggcggcc tcggcatggg
     1381 gcagcagcac gatgccgacc ccggcgagga ccgcgttgac caccacgagg agggcgatcg
     1441 cgccgcgggc gccggcatcg gaccgcagga cccggcccgc cccctgggcc ggctcccgag
     1501 gcacgtgctc aggtcgcggc ataccagtcc ccgcgaacag cagcgcggcc gcaccgacga
     1561 gtgccgcgcc gacaccgagg accatcggcg cggcggctgc ccccatgagg agcccgccca
     1621 gagccgggcc gacgacgaac gaagccacct cgaccgtcac gagcaggtcg gtggcgcggt
     1681 cgttgtgacg tccggcaagc gtgggcagcc cggccgcgag ggaggggtag gccggcgtcc
     1741 ccgcggccac ggtcagtgtg aggagtacca cgcaggtggc cgtccgaccg gttgcgacag
     1801 cctgggaggc cgcagccagc agcactagcc gagctcccag ggtccaccag acaacccggt
     1861 cgcgcgccca gcggtcggcg agtcggccgg cgagccagga gatcgcgaca tacgggagca
     1921 tccgggccgc accgacgaga ccaagcaggc gttcgtcgcc ggtctcgtgc cagacccgcg
     1981 ccatgagcag aggccacggc agggacatgc ccacggcggc caacccgtga ctggcgacca
     2041 cgcgccagac ccgtcggccc atgccaggtg agaggcctgg ccgtgagcgg gtgatacggc
     2101 gggcctacgt cgggcggtcg acgacgggtg gcaccacggc cagtgcctcg tcgacagaca
     2161 ggccggtgac ctgaagcagg tcgatgacgg tcgaccgcag ctgggcgagg agaacttcgg
     2221 tcgagaggta tgacgtgcgc accagagccg cggtccgctc accgacccgc accagctcgg
     2281 accggccgac ctccggggag gcgttctccc ccagggcgcg ggcgaggatg tcggtcgccg
     2341 tggcgaggtc ggtgagcaca gcgacacacg aggcagggac cggaccatcg gaccgcgtgg
     2401 cgacgacaac ccgccggatg aggacccggg tgctgcgcag ggcgcggtcg atcgggtcga
     2461 tgaggtcggc gaccttgcgg acctggaccg catggtggcg gtgcagcggc gaggaggcca
     2521 gcac
//
LOCUS       DATAIX010000502         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_220435, whole genome shotgun sequence.
ACCESSION   DATAIX010000502 DATAIX010000000
VERSION     DATAIX010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220435"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VHM65_07540"
     CDS             <1..198
                     /locus_tag="VHM65_07540"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HEX2705594.1"
                     /translation="GLVPALPALRIVSAGNVAGEGAKMALLSSRERAGADALLEEVRY
                     VELSDRTDFNDRFVDLLSFPS"
     gene            260..859
                     /locus_tag="VHM65_07545"
     CDS             260..859
                     /locus_tag="VHM65_07545"
                     /inference="COORDINATES: protein motif:HMM:NF019413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HEX2705595.1"
                     /translation="MARHTWPVDVFPLPPLLHNRPHRIAGEVERLALDLRERYGRVVV
                     GYADCGTYGALDALCERLGLTRLAGLHCYDLCAGAERVRAIFEAEPGTYVLTDFLVRS
                     FARTVIAELGLDRYPELRDDYFGSYHRVVWLAQEPDPGLRALAQEAADRIGLPLAVVP
                     TGRSGLERALAEVLGPEATPSVLGPAQPAGAQQVANWRR"
     gene            complement(832..1782)
                     /locus_tag="VHM65_07550"
     CDS             complement(832..1782)
                     /locus_tag="VHM65_07550"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2705596.1"
                     /translation="MLRTVAVIVQEPVAAFELGVLSEVFGIDRTDDGVPAFEFRVCAA
                     RPGEVLETTGGFGVVARHPLDEARDADLVAIPAGPVDGPFDDAVLDVLRASTERGTRI
                     LSVCTGAFTLAAAGLLDGRRCATHWRYAERLARAYPSAQVDLDVLYVEDGPVLTSAGT
                     AAGIDACLHLVRAELGAQVATRIARRMVVPPHRGGGQRQYVETPILQHSCDSLQPVLD
                     WVGANLGTDHPVPALAERAQMSERTFARRFLHEVGTTPHRWVTEQRVLRARTLLETTD
                     LDIEAVAHACGLASGAVLRQHFQRELGVSPATYRRQFATC"
     gene            1865..2116
                     /locus_tag="VHM65_07555"
     CDS             1865..2116
                     /locus_tag="VHM65_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705597.1"
                     /translation="MNTLLLVLMLAVLVATLERTHRRGGSLWPRPFDGEALSTDNRDL
                     SRTRQELVAGDAGAGRRPPEGPRPAGASEVCCPPPAGTA"
BASE COUNT          281 a          781 c          766 g          318 t
ORIGIN      
        1 ggtctcgtcc ccgcgctgcc cgccctgcgc atcgtctccg ctggcaacgt cgccggcgag
       61 ggggccaaga tggcgctgct cagctcacgt gagcgggccg gtgccgacgc gctgctcgag
      121 gaggtgcgct atgtcgagct ctccgaccgg accgacttca acgaccgctt cgtcgacctg
      181 ctctccttcc ccagctgacc tgtccgcccg cattgcgctc gtcgcgtgcg gtgcgatcgc
      241 cgcgccggcc gctgacgccg tggcgcgcca tacctggccg gttgacgtct tcccgctgcc
      301 tccgttgctg cacaaccggc cgcaccggat cgcgggtgag gtggagcggc tcgccctgga
      361 cctgcgcgaa cgctacggcc gcgtcgtcgt cggctatgcc gactgcggga cgtatggcgc
      421 gctcgacgcc ctgtgcgagc gcctcgggct cacccggctg gctgggctgc actgctacga
      481 cctgtgcgcc ggggccgagc gggtgcgggc gatcttcgag gccgagccgg ggacgtatgt
      541 gctgaccgac ttcctcgtgc gctcgttcgc ccggacggtc atcgccgagc tcgggctcga
      601 tcgctatccg gagctgcgcg atgactactt cggcagctac caccgggtcg tgtggctggc
      661 gcaggagccc gatcccgggc tgcgggcgct cgctcaggag gccgcggacc ggatcggcct
      721 gcccctggcg gttgttccga cggggcgaag cgggctggag cgggcgctcg ccgaggtgct
      781 cggcccggag gcgaccccct cggtgctcgg ccccgcgcag cctgcaggcg ctcagcaggt
      841 cgcgaactgg cgccggtagg tcgccggtga gacgcccagc tcgcgctgga agtgctggcg
      901 cagcaccgcg cccgaggcca gtccgcaggc gtgcgcgacc gcctctatgt ccaggtccgt
      961 ggtctccagc agcgtgcggg cccgcaggac acgctgctcg gtcacccacc tgtgcggcgt
     1021 cgtcccgacc tcgtggagga agcgccgggc gaaggtccgc tcggacatct gggcccgctc
     1081 ggcgagcgcg ggcacggggt gatcggtccc caggttcgcc ccgacccagt caagcaccgg
     1141 ctgcaggctg tcgcacgagt gctgcagaat cggggtctcg acgtactgcc gttggccacc
     1201 cccgcggtgc ggtggcacga ccatccggcg ggcgatccgg gtcgcgacct gggccccgag
     1261 ctcggcgcgc accaggtgca ggcaggcgtc gatgccggct gccgtcccgg cactggtgag
     1321 gacgggtccg tcctcgacgt agaggacgtc gaggtcgacc tgggcgctcg ggtaggcgcg
     1381 agccaggcgt tcggcatagc gccagtgggt tgcgcaccgg cgcccgtcga gcaggcccgc
     1441 ggcagcaagg gtgaacgcgc cggtgcacac ggacaggatc cgcgtgcctc gctccgtgct
     1501 cgcgcggagc acatccagga ccgcgtcgtc gaaaggtcca tcgaccggac ctgccggtat
     1561 ggcgaccagg tccgcgtcgc gtgcctcgtc gagggggtgc ctggccacaa cgccgaaccc
     1621 gcccgtggtc tccaggacct caccagggcg cgccgcacag acccggaact cgaaggcagg
     1681 gacgccgtcg tcggtccggt cgatgccgaa cacctcggac agcacaccga gctcgaaggc
     1741 ggcgacgggt tcctgcacga tgacggccac ggtgcggagc atgagggcag tgtggcagaa
     1801 aaggtacgca ctgcgccttt cctgccactg gtggccgaat taacgtgtcc gcataattac
     1861 agacatgaac actctcctgc tcgttctcat gctcgccgtc ctcgtcgcca ccctcgagcg
     1921 gacccaccgc cggggcggct ccctctggcc tcggccgttc gacggcgagg cgctgtccac
     1981 cgacaaccgt gacctgtccc gtacccgtca ggagctcgtg gccggagacg ccggcgcggg
     2041 gcgccgtccc cccgagggcc cgaggccggc cggcgccagt gaggtgtgct gcccgccacc
     2101 agcgggcacg gcatagtctc tggcgcactc acacctgctc gccccg
//
LOCUS       DATAIX010000503         2218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_116698, whole genome shotgun sequence.
ACCESSION   DATAIX010000503 DATAIX010000000
VERSION     DATAIX010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116698"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..>2218)
                     /locus_tag="VHM65_07560"
     CDS             complement(112..>2218)
                     /locus_tag="VHM65_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2705598.1"
                     /translation="LCGRFDPELCRLFTAALQTGRSSDELLTALRAHSPAPSLGAVRA
                     PTLLIQGMADSLFGLDQADATARALAAGGTPVAVRWIDGGHDGASSTATADDAAAQAW
                     IDHYLRGATPTSGLAVPAFVYAEPIPRRSSAAPLRSLAAYPGLGAALSLTRVALPLKA
                     AQPQTLLTPPGGQPASVTTIPGLGALGASIAPYQLAALRGQSAAFDTAPVPSRTGVVG
                     APRVRLRVTSTGEVVTLFVSLWQVNGASATLPRRLVAPVQVPVVPGRSTLIEVVLPAA
                     TYTLESGSTWRVLVTSTDSAYAGPREARADRVSLAATVLDLPVATGAALTTAADRDGE
                     ALGVAGALAGVLLMLTGLWWWRRRRRRRLADRPDLHGVPMVIEGLVKTYADGHRAVDD
                     VSWRAEQGQVVGLLGPNGAGKTTTMRMALGLIRPDAGTVHVMGEPVSAGAPVLGRVGA
                     LVEGPGFLPHLTGRQNLQAYWAATGRAPGEACYAEVLEVAALGNAVERPVRSYSHGMR
                     QRLGIAQAMLGMPDLLVLDEPTNGLDPPQIAAMRPILHAYAATGRTVVISSHLLAEVE
                     MTCSHVVVMHAGRVVMTGPVAELLDSADTTVVTLAPGSDPERVATSLRGLDVVTAVEV
                     DLDPAAPRIVVTATADRPHVVRAVGALGADIVGVTSRRHLEEVFLGVIAQAQGAEPAA
                     PGAEGARSLSDRLRQVRAR"
BASE COUNT          308 a          809 c          830 g          271 t
ORIGIN      
        1 gccgcgcacc gtcatacgtg cgccgtccgc tctgctggcg gccccattcc gcgcgccagc
       61 agagcggacg gcgcacgtat gacggtgcgc ggccggcgcc ctggtccggg gtcatcgggc
      121 ccgcacctgc ctgagccggt cgctcagtga gcgagccccc tcggcgccgg gtgccgcagg
      181 ctcggccccc tgagcctggg cgatgacgcc gaggaacacc tcctcgaggt gacgccggct
      241 cgtcaccccg acgatatccg cgcccagcgc gccgaccgcc cggacgacgt gcgggcggtc
      301 agcggtcgcc gtgacgacga tccggggggc cgccgggtcg aggtccacct caaccgctgt
      361 cacgacgtcc agtccacgaa gggaggtggc cacccgctcg gggtccgacc ccggtgccag
      421 cgtgacgacg gtcgtgtcgg cgctgtcgag cagctcggcc accggaccgg tcatgacgac
      481 gcgcccggcg tgcatgacga cgacatggct gcacgtcatc tcgacctcgg cgaggagatg
      541 actcgagatc accacggtcc ggccggtcgc ggcataagca tgaaggatcg ggcgcatcgc
      601 ggcgatctgc ggcgggtcga ggccgttcgt gggttcgtcg aggacgagca ggtcgggcat
      661 ccccagcatg gcctgggcga tgccgaggcg ctggcgcatg ccgtggctgt atgaccgcac
      721 cggtcgctcg accgcgttcc cgagcgcggc aacctcgaga acctccgcat agcaggcctc
      781 cccgggcgcc cggccggtcg cggcccagta cgcctgcagg ttctgccggc cggtcaggtg
      841 aggcaggaac cccggcccct cgacgagcgc tccgacccgg ccgagcacgg gtgccccggc
      901 ggagacgggt tcgcccatga catgcacggt tccggcatcg ggccggatga ggccgagcgc
      961 catccgcatc gtcgtcgtct tgccggcccc gttcgggccc agcagcccga cgacctgccc
     1021 ctgttccgcc cgccagctga cgtcgtcgac cgcgcggtgg ccgtcggcat aggtcttgac
     1081 cagtccctcg atgaccatcg gaaccccgtg caggtcgggg cggtcggcga ggcggcgacg
     1141 gcgccgccgg cgccaccacc acaggccggt cagcatgaga aggacacccg ccagagcccc
     1201 ggccaccccg agggcctcgc cgtcacggtc tgccgcggtc gtcagggccg cgcccgtcgc
     1261 cacggggagg tcgagtaccg tcgcggcgag gctgacccgg tcggctcggg cctcgcgggg
     1321 accggcatac gccgagtcgg tgctcgtgac gaggacccgc cacgtgctcc ccgactcgag
     1381 ggtgtacgtc gcggccggca agacgacctc gatgagcgtg gaccggcccg gcacgaccgg
     1441 gacctggacc ggcgcgacga gccggcgcgg cagggtggcc gacgcgccgt tgacctgcca
     1501 gagcgagacg aaaagggtca ccacctcgcc cgtcgaggtg acgcgaaggc gcacccgggg
     1561 cgccccgacg acgccggtcc gggatggcac gggtgcggtg tcgaaggcgg ccgactgacc
     1621 gcgcagcgcg gcgagctggt atggcgcgat gctggctccc agcgccccga ggccggggat
     1681 cgtcgtcacc gacgcgggct ggccgccggg aggtgtgagg agcgtctgcg gctgagccgc
     1741 cttgagtggc agggcaaccc gggtcaggct cagtgcggct ccgagcccgg ggtaggccgc
     1801 caggctccgc agtggtgcgg cactcgaccg ccgggggatc ggctcggcat agacgaacgc
     1861 cggcacggca agcccgctcg tgggcgttgc cccgcgcagg tagtggtcga tccacgcctg
     1921 ggccgcggca tcgtccgccg tcgccgtgga gctggccccg tcgtgtccac cgtcgatcca
     1981 gcggaccgcc acaggggtcc ctcccgcggc aagcgcgcgc gccgtcgcat cggcctggtc
     2041 gagcccgaag agcgagtctg ccatgccctg gatcagcagg gtcggagccc ggaccgcacc
     2101 gagtgacggg gccgggctgt gggcacggag cgcagtgagc agctcgtcgc tggatcgccc
     2161 ggtctgcagg gcagcggtga agagccggca cagctccggg tcgaaccgcc cgcacagg
//
LOCUS       DATAIX010000504         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_116873, whole genome shotgun sequence.
ACCESSION   DATAIX010000504 DATAIX010000000
VERSION     DATAIX010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116873"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VHM65_07565"
     CDS             complement(<1..385)
                     /locus_tag="VHM65_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013283714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2705599.1"
                     /translation="MTPGSSVPDLSGPSDPSDLAHLSNDLRLTCMRIARRVRFEATDA
                     LAPHLFSVLVRLAAGPLTPGELAEIERVSAPSMTRTVAALLVRGLVTRTADPVDRRQV
                     IVAITVEGSGLVAATRAQREAWMASR"
     gene            complement(440..1924)
                     /locus_tag="VHM65_07570"
     CDS             complement(440..1924)
                     /locus_tag="VHM65_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEX2705600.1"
                     /translation="MPTPTRAPARPAASGLDDFFSITKRGSTVARELRGGLVTFFTMA
                     YIVVLNPLIIGTQADSTGKFLGGGTTPNLALVAAATALIAGVMTILMGVVANYPLALA
                     TGLGLNAFVAFGIAKLPQMTWADAMGLVVLEGVIILILVLPGFRKAVFHAVPAQLKTA
                     ISVGIGLFIALIGLVDAGFVRKAPAVPVEMGVGGVLAGWPLLVFVLGLGAIVTMMVRG
                     VKGAILYGIVGATVLAVLVEAVFTVGRQTDATGKIVNPTGWGLNRPAFPDKVVDLPDF
                     GLLGDFTLFGSFAKIGFVSAALLVFTLMLADFFDTMGTMVAIGAEAGLLDREGNPPHA
                     EKILIVDSVAAIAGGAGSVSSNTSYIESAAGVGEGARTGLASVVTGVLFLVATFLAPL
                     VAIVPYEAATPALVVVGFLMMQQVKGIDWDDVEIAIPAFLTIVLMPFTYSITVGIGAG
                     FVAYVLIKVVRGKASRIHPLLWVVSALFLVYFAIDPLKSLLGVS"
     gene            2027..2260
                     /locus_tag="VHM65_07575"
     CDS             2027..2260
                     /locus_tag="VHM65_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705601.1"
                     /translation="MSSEQGPGPTEPMRGLTDRIAQTGVLIWAAALAATLVIPSWHAG
                     ERSWWPWAACAGVVIGLLGWGYLRRGRGNAAGL"
     gene            complement(2322..>2566)
                     /locus_tag="VHM65_07580"
     CDS             complement(2322..>2566)
                     /locus_tag="VHM65_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705602.1"
                     /translation="FLQGVTDFYRARTDTKMTIAAERLAVIAAITLPVTATSSVVGMN
                     VIVNDSTHVGLLVALLVLMAAMSLILLRWAKRQGWW"
BASE COUNT          437 a          854 c          938 g          337 t
ORIGIN      
        1 cccgggacgc catccaggcc tcgcgctggg cccgggtcgc cgcgacgagc ccggagccct
       61 cgacggtgat cgcgacgatg acctggcgcc ggtcgaccgg gtcggccgtg cgggtgacca
      121 gaccgcggac gagcagcgca gcgacggtgc gggtcatcga gggagcgctc acccgctcga
      181 tctcggccag ctcgcccggg gtgagcgggc ccgcggcgag tcgcacgagc acggagaaca
      241 ggtgcggcgc gagcgcgtcc gtggcctcga agcgcacccg gcgggcgatc cgcatacagg
      301 tcagacgcag gtcgttcgac aggtgggcca ggtcggacgg gtcggacggg ccggagaggt
      361 cgggcacgct cgaaccaggg gtcaccgtca cactatggca aactcgttac cttggctaac
      421 tattccaccg gtcgagcgtt cagctcacgc cgagcagtga cttgagcggg tcgatcgcga
      481 agtagacgag gaacagtgcc gagaccaccc acagcagcgg gtggatcctc gatgccttgc
      541 cgcggaccac cttgatgagc acgtaggcga cgaacccggc gccgatgccg accgtgatcg
      601 agtaggtgaa cggcatgagc acgatcgtga ggaacgcggg gatggcgatc tccacgtcgt
      661 cccagtcgat gcccttgacc tgctgcatca tgaggaagcc gacgacgacc agcgccggcg
      721 tggccgcctc gtacgggacg atcgcgacga gtggcgcgag gaaggtcgcg accaggaaga
      781 gcactccggt cacgaccgag gccaggcccg tgcgcgcgcc ttcgccgaca cccgcggccg
      841 actcgatgta tgacgtgttg ctgctgacgc tgccggcccc acccgcgatg gcggcaaccg
      901 agtcgacgat gaggatcttc tccgcgtggg gcgggttgcc ctccctgtcc agcagaccgg
      961 cctcggcgcc gatggcgacc atcgtgccca tcgtgtcgaa gaagtcggcc agcatgagcg
     1021 tgaagacgag cagggctgcc gagacgaacc cgatcttggc gaacgacccg aacagggtga
     1081 agtcaccgag cagtccgaag tcgggaaggt cgacgacctt gtccgggaag gcaggccggt
     1141 tcaggcccca gcctgtcggg ttgacgatct tgccggtggc gtcggtctgg cggccgaccg
     1201 tgaagacggc ctcgacgagg acggcgagca cggtcgcgcc gacgatcccg tagaggatgg
     1261 cgcccttgac cccccgcacc atcatcgtga cgatcgcgcc gagcccgagg acgaagacga
     1321 gcagcggcca tccggccagg accccgccga cgcccatctc gaccgggacg gccggggcct
     1381 tgcggacgaa gcccgcgtcg accagcccga tgagggcgat gaacagcccg atcccgaccg
     1441 agatcgcggt cttgagctgg gccggcacgg cgtggaagac ggcctttcgg aagccgggga
     1501 gcacgaggat gaggatgata acgccctcga ggacgaccag gcccatggca tcggcccacg
     1561 tcatctgggg cagcttggcg atcccgaagg ccacgaacgc gttgaggccg agcccggtcg
     1621 ccagcgccag ggggtagttg gcgacgacgc ccatgaggat ggtcatgact ccggcgatga
     1681 gcgcggtcgc cgcggcgacg agggcgaggt tcggcgtcgt gcctccgccg aggaacttcc
     1741 cggtcgagtc ggcctgggtg ccgatgatga gcgggttgag gaccacgatg taggccatcg
     1801 tgaagaacgt cacgagacca ccgcgcagct cacgggcgac ggtcgacccg cgcttggtga
     1861 tcgagaagaa gtcgtcgaga ccggacgccg ccggccgggc cggcgctctg gtgggggtag
     1921 gcatggcgca cagactagtg cggccatgtt tcgctcgcgt gacgcgcccg gatgcctcgc
     1981 gctgcccccg cggcccggcg gcgggcccgc ggcgtagggt ggcgctgtga gcagcgagca
     2041 ggggcctggc cccaccgagc ccatgcgggg gctcaccgac cggatcgccc agaccggggt
     2101 cctcatctgg gccgccgcgc tcgccgccac gctcgtcata ccgtcctggc atgccgggga
     2161 gcggtcgtgg tggccgtggg cggcttgcgc cggggtggtg atcggcctgc tcggctgggg
     2221 ctacctgcgc cggggccggg gcaacgccgc cgggctgtag gtcaccacca gccatgtcac
     2281 caccagccag gtcaccacca gccaggtcac caccagcgag gtcaccacca gccctggcgt
     2341 ttggcccagc ggagcaggat cagcgacatc gccgccatga ggaccaggag cgcgacgagc
     2401 aggcccacgt gggtcgagtc gttgacgatg acgttcatcc cgacgaccga gctcgtggcc
     2461 gtgaccggca gggtgatcgc cgcgatcacc gcgagccgct cggccgcaat cgtcatcttg
     2521 gtgtcggtcc gggcccggta gaagtcggtc acgccctgca ggaacg
//
LOCUS       DATAIX010000505         2377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_27134, whole genome shotgun sequence.
ACCESSION   DATAIX010000505 DATAIX010000000
VERSION     DATAIX010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27134"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1031)
                     /locus_tag="VHM65_07585"
     CDS             complement(135..1031)
                     /locus_tag="VHM65_07585"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX2705603.1"
                     /translation="MGDLLDTMHRAGGCATFEHLRVHHSRRAVRAALADGAIVRLRRD
                     RYALGGVEVHRREAHRMSATLSHLSAAAAYGWGLKTAPPRPMVTVPKHRTVTATDQAR
                     AVVSWRDLAASDRVGGLTSPLRTVLDCAVSLPFDEALAVADSALREGDVEHEALVAAA
                     ATLRGPGSARARRVARLADGRAANAFESVLRAIAMDVEGFHFVPQVQIWDSGLWATVD
                     LADESARLVLEAEGFATHGTRAGLVRDCHRYTELVAWGWEVLRFTWEDVMFEPQWVRW
                     AIQAVAERRRGDSVSPPPRRTT"
     gene            complement(1225..>2377)
                     /locus_tag="VHM65_07590"
     CDS             complement(1225..>2377)
                     /locus_tag="VHM65_07590"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002512649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX2705604.1"
                     /translation="ADAYDGLTRKPVVAFGSSLAGDLVRRDFTVNAMALRLPSLVFVD
                     SYGGLADIAARRLRTPSTPEVSFADDPLRMMRAARFTAQLGFSVAPDVRAAMESLAPT
                     LTIVSAERVRDELVKTLLAPRPRDGLTLLVDTGLAGQFLPELPALRLEVDEHHRHKDV
                     YEHSLTVLEQAIMLEGPPGSDAGLVPGPDLVLRLAALLHDIGKPATRRFEPGGGVSFH
                     HHEMVGAKLSTRRLKALRFDNDTTTAVARLVELHLRFHGYGEGQWTDSAVRRYVTDAG
                     PLLARLHRLTRSDSTTRNRRKAERLSRTYDALESRIEVLMAQEELRTVRPELDGTAIG
                     EVLGIPPGPVLGRAYRYLLAVRLDEGLIGPEAARERLLAWWAEQPESHA"
BASE COUNT          360 a          851 c          861 g          305 t
ORIGIN      
        1 ggtcgcggcc acccgaacag gcccaacgtc tccaggattc ggacatactg gggcacgacg
       61 gacaccaggt gggtgttggg ccagttgggc ggtccgccga cactggcagc gaggcccagc
      121 ggagcctgcc gcactcaggt agtgcgccgc gggggcgggg acaccgagtc accgcggcgc
      181 cgctcggcga cggcttggat ggcccaccgc acccactgag gctcgaacat cacgtcctcc
      241 caggtgaagc gcagcacctc ccatccccag gcgacgagct cggtgtagcg gtggcagtcc
      301 ctgaccaggc ccgcgcgggt gccgtgcgtc gcgaacccct cggcttcgag cacgagccgg
      361 gccgactcgt cagcgagatc gacggtggcc cacagaccgc tgtcccagat ctggacctgc
      421 ggcacgaagt ggaacccctc gacgtccatg gcgatcgccc gcaggaccga ctcgaacgcg
      481 ttggccgctc gcccgtcggc cagccgggcc acccggcgag cccgggccga gcctggccct
      541 cggagcgtgg cggccgccgc caccaacgcc tcgtgctcga catcgccctc ccgcagtgcc
      601 gagtcggcga ccgcgagcgc ctcgtcgaag ggcaggctca ccgcgcagtc gaggacggtg
      661 cgcaggggtg aggtgagccc gccgacccga tcggatgccg cgaggtcgcg ccaggagacg
      721 acggcccgcg cctggtccgt cgccgtcacg gtgcggtgct tcggcacggt caccatcggc
      781 cgcggcggag ccgtcttgag gccccagccg tatgccgccg ccgcgctcag atgcgacagc
      841 gtcgccgaca tccgatgcgc ctcgcgccga tgcacctcga cgccgcccag ggcgtaccga
      901 tcacggcgca gccggacgat ggcgccgtcg gccagggccg cgcggaccgc ccggcgtgag
      961 tgatgcaccc tgaggtgctc gaaggtcgcg cagcctccgg cccggtgcat cgtgtcaaga
     1021 aggtcaccca ctcgacgagt gtgagacacc ccggctcccg gccgccgacg ttatccacag
     1081 cctgtggata acgctggaac tggtcgaaca gcaccagccg gagcgcctcc acgctcagtg
     1141 cgcccacgac ggggctcgtt cgacccgttc gggagtccgc ccggagacgg aggctcaggc
     1201 gaggcacccg gagcctgcgc cggctcaggc gtgggactcg ggctgctcgg cccaccaggc
     1261 caggaggcgc tcccgggccg cttcggggcc gatcagtccc tcgtcgaggc gtaccgcgag
     1321 caggtaccgg taggcacgac cgaggacagg accagggggt atgccgagca cctcaccgat
     1381 cgcggtgccg tcgagctcgg gtcgcacggt cctgagctcc tcctgcgcca tgaggacttc
     1441 gatccgggac tcgagcgcgt catacgtgcg ggacaggcgt tcggccttgc gccggttgcg
     1501 cgtcgtgctg tccgagcggg tgagccggtg caggcgggcg agcagcggcc cggcgtcggt
     1561 gacatagcgc cgcacggccg agtcggtcca ctgaccttcg ccatacccat ggaagcgcag
     1621 gtgcagctcg acgagccgcg cgaccgccgt ggtggtgtcg ttgtcgaacc gcagcgcctt
     1681 gagccggcgg gtgctgagct tggccccgac catctcgtgg tggtgaaagc tcaccccacc
     1741 gccgggctcg aaccggcggg tggccggctt gccgatgtcg tgaagcagcg cggcaaggcg
     1801 cagcacgagg tcgggtcccg gcacgaggcc ggcgtccgac ccgggcggcc cctcgagcat
     1861 gatcgcctgt tccagcaccg tgagcgagtg ctcgtagacg tccttgtgcc ggtgatgctc
     1921 gtcgacctcg aggcgcagcg cgggcagctc gggcaggaac tgcccggcca gcccggtgtc
     1981 gacgagcagg gtcaggccgt cccgcggacg aggcgccagc agcgtcttga ccagctcgtc
     2041 ccggacccgt tcggccgaca cgatcgtcag cgtcggggcg agcgactcca tcgcggcccg
     2101 gacgtcgggg gccacgctga acccgagctg ggcggtgaac cgcgctgccc gcatcatccg
     2161 cagcgggtcg tcggcgaagg acacctcggg ggtcgagggt gtgcgcaggc gccgcgccgc
     2221 gatgtccgcc agaccgccat aggagtcgac gaagaccaga gacgggagcc gcagcgccat
     2281 cgcgttgacc gtgaagtcgc gccggacgag gtcgccggcg agtgacgagc cgaatgcgac
     2341 aacgggtttg cgcgtcagcc cgtcataggc atcggcc
//
LOCUS       DATAIX010000506         2055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_116942, whole genome shotgun sequence.
ACCESSION   DATAIX010000506 DATAIX010000000
VERSION     DATAIX010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116942"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1914)
                     /locus_tag="VHM65_07595"
     CDS             complement(<1..1914)
                     /locus_tag="VHM65_07595"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HEX2705605.1"
                     /translation="MSAPAPSSAAQLDAAITTVSIREESSGLFDRLHVDLAWQVPDGS
                     HAGDTFRLTLPSSLRMRATVSFDLTDPTDAVVAHAHASGSTAVFTLTEYAEAHVGVEG
                     TAWFEVNWNSQVLDPGTKQTVAITVAGSTFTDTVDIDPRPTNAEPLKKMSWVDDDEVR
                     LGWSIVAGLVTPDQVGSTLVLTETAGDGQAVDCSTVHVIFGSYRDKGTITNGATYPRA
                     TWTSSSCSVAAASVRLTVPASAVGKTMLLTGRSDVTDPKQTKFRNTGKVVIDGKGTPV
                     STTTVRASGGGTGYGNKSVSVGDLVWLDANHNGIQDGAERGIAGVTLRLVGPSGAGVT
                     SLRDTPVGAVVTDSAGHYSFTDLPALGAGQSYTVTVTPPTGPVATLTGAGTAATDSSA
                     GSASSGDLTVNDASDLTLDFGYWEPIPAIRVLEGDVRGNAADTQAHAVTLPNGSTGLE
                     LTVTNTGNEALTDLAVSDLVLAGGTVAGLTCDFSAVGGPAVGTTWSGPLAAGASFPCT
                     AALSGVDPGAVHEDIATVTGTGAVSGTTVKDDNAYHGTRPKPGVTVVKGDTHGNTADT
                     ALSAASVPSGSAGLVLTVKNVGSEVLESIIVTDLVVSNGIVTGLACDLSTLGGPATGT
                     TWAGPLHPGESFGC"
BASE COUNT          296 a          715 c          739 g          305 t
ORIGIN      
        1 gcacccgaac gactccccgg ggtgcagcgg tcccgcccaa gtggtccccg tggccggccc
       61 accgagcgtc gacaggtcac aggccaggcc cgtgacgatg ccattgctca cgacgaggtc
      121 ggtcacgatg atcgactcca ggacctcgga gccgacgttc ttcacggtga ggaccagtcc
      181 ggccgagccc gagggaacgc tggcggccga caaggcggtg tcagcggtgt ttccgtgggt
      241 gtcgcccttg accacggtga cacccggctt gggccgggtc ccgtggtagg cgttgtcgtc
      301 cttgaccgtc gtgccactca cggcgcctgt cccggtgacg gtggcgatgt cctcgtggac
      361 ggccccaggg tccacgccgc tcagcgcggc ggtgcagggg aaggacgcgc cggccgcgag
      421 ggggccggac cacgtggtgc cgacggcggg tccgcccacg gcggagaagt cgcaggtgag
      481 gccggccacg gtcccgccgg ccaggacgag atcgctgacc gcgaggtcgg tgagggcctc
      541 gttgcccgtg ttcgtcacgg tgagctccag gccggtcgac ccgttcggga gcgtcactgc
      601 gtgagcctgc gtgtcggcag cgttgccgcg aacgtcaccc tcgaggaccc ggatggcagg
      661 gatcggctcc cagtagccga agtccagggt gaggtccgac gcgtcgttga ccgtgaggtc
      721 accggaggac gcagacccgg ccgaggagtc cgtggccgcc gtcccggccc cggtgagggt
      781 cgcaacgggg ccggtcggcg gcgtcaccgt caccgtgtag gactgtccgg cgccgagggc
      841 cgggaggtcg gtgaagctgt agtgcccggc cgaatcggtc accacggccc cgaccggcgt
      901 gtccctcagc gatgtcacgc cggccccgct ggggccgacg agccgaaggg tcacacctgc
      961 gatccctcgt tcggcgccgt cctggatgcc gttgtggttg gcgtccagcc agacgaggtc
     1021 gcccaccgag acgctcttgt tcccgtagcc ggtccctccg ccgctggcgc gcacggtcgt
     1081 cgtggacacc ggggtccctt tcccgtcgat gacgaccttc ccggtgttgc ggaacttggt
     1141 ctgcttcggg tcggtcacgt cggaccggcc ggtcagcagc atcgtcttgc cgaccgccga
     1201 ggccgggacc gtcagtctga ccgacgccgc ggcgaccgag cacgacgaac tggtccaggt
     1261 ggcccgcggg taggtggcgc cgttcgtgat cgtccccttg tcccggtagg agccgaagat
     1321 gacgtgcacc gtcgagcagt cgacggcctg gccgtccccg gcggtctcgg tgagcacgag
     1381 agtcgacccg acctggtccg gcgtgaccag cccggcgacg atcgaccagc ccagccgcac
     1441 ctcgtcgtcg tcgacccacg acatcttctt gagcggctcg gcattcgtgg gacgcgggtc
     1501 gatgtcgacc gtgtcggtga aggtcgagcc cgcgactgtg atcgccacgg tctgcttcgt
     1561 ccccgggtcg agcacctggc tgttccagtt cacctcgaac cacgccgtcc cctcgacccc
     1621 gacatgggcc tcggcatact cggtcagggt gaacacggcc gtgctaccgg aagcgtgcgc
     1681 gtgggccacg acagcgtcag tggggtcggt caggtcgaag gacacggtgg cgcgcatcct
     1741 gagcgagctc ggcaaggtca gccggaaggt gtcacctgcg tgactgccgt ccgggacctg
     1801 ccacgccagg tcgacgtgga gccggtcgaa caggccagag gactcctccc ggatcgacac
     1861 cgtcgtgatc gccgcatcca gctgcgccgc cgacgagggc gccggggctg acacagcgcc
     1921 gagagcggcg accagcccca ccaccagcgc gacagcgagc cggacgaaac cgagccggag
     1981 gacattcggt cggccgatcc ggctccgtat gttccgcgcg ttccgcggcg cgcgcgagcg
     2041 cgcaccatcg aggac
//
LOCUS       DATAIX010000507         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_117011, whole genome shotgun sequence.
ACCESSION   DATAIX010000507 DATAIX010000000
VERSION     DATAIX010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117011"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1590)
                     /locus_tag="VHM65_07600"
     CDS             complement(<1..>1590)
                     /locus_tag="VHM65_07600"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX2705606.1"
                     /translation="EFNAGAMENPACVTIRDQYLFRGAATGDEIQTRDNTIAHEMAHM
                     WFGDLVTMRWWDDLWLNESFAEYLSHRALVEATRHTDAWVGSTIIRKAWGYAAERAPS
                     THPVAGGLALDAQSALQDFDGIAYAKGAAVLRQLIAYVGDAAFVQGLRHHVQAHAFGN
                     ADMGDLLASMERSSRLDLASWRAAWLTTAGRDEISAERRGGQLVLRRVTPARHPADRP
                     HTLDTAGYSRGREVFRVPATIHSEWTQLDVVPPAAVVVPNASDLTWAAVRLDDDTVAA
                     LPDELALIPDVQVRATIWVALVDSVAQGLVDPAVFFAVLAAAWPHETHAAIMHRVALV
                     AAKRFLPHFTAPAMREPTRHLLSVAGEATLELAEPGSSTALGAARLVAQVSADRERLA
                     RWLAGERLPRGLEHDSDFRWLVLGNLAYRGWVATAEIEAARAGDSTLQGALNALCCRA
                     SLPDAGSKAWAWEQLTSEEAGRSNYELNHLAEGFWRADDDSVVRPYVARYFEEIPLLA
                     GWVGEDALARVVLLAFPKVIED"
BASE COUNT          244 a          590 c          549 g          207 t
ORIGIN      
        1 gtcctcgatg accttgggga aggccaggag cacgacccgg gccagcgcgt cctccccgac
       61 ccacccggcg agcagcggga tctcctcgaa gtaccgcgcg acatagggcc ggaccaccga
      121 gtcgtcatcg gctcgccaga aaccctcggc gaggtggttg agctcatagt tcgaccggcc
      181 cgcctcctcg ctggtcagct gctcccacgc ccaggccttt gaccccgcgt cgggcagcga
      241 ggcccggcaa cacagcgcgt tgagggcacc ctgcagggtc gagtcgcccg cacgggccgc
      301 ctcgatctcg gcggtggcga cccagccccg gtaggcgagg ttgcccagga cgagccagcg
      361 gaagtccgag tcatgctcga gcccgcgggg cagccgctcc cccgcgagcc accgggccag
      421 ccgctcccgg tcggcggaca cctgcgcgac gaggcgggcg gcaccgaggg ccgtcgacga
      481 tcccggctcg gccagctcca gcgtggcctc gcccgcgacg gacagcaggt gacgggtcgg
      541 ctcgcgcatg gccggcgccg tgaagtgggg caggaagcgc ttggcagcca cgagcgcgac
      601 gcggtgcatg atcgccgcgt gcgtctcgtg cggccaggcc gccgccagca ccgcgaagaa
      661 caccgccggg tcgaccaggc cctgcgcgac cgagtcgacg agcgcgaccc agatcgtggc
      721 ccggacctgc acgtcgggga tgagcgcgag ctcatccggc agggcggcca ccgtgtcgtc
      781 gtcgaggcgc accgctgccc aggtgagatc ggacgcgttc ggcacgacca cggccgcagg
      841 cggcaccacg tcgagctggg tccactccga gtggatcgtg gccggcaccc ggaagacctc
      901 gcgcccgcgc gagtagccag cagtatcaag ggtgtgcggc cggtcagccg gatggcgggc
      961 cggcgtgaca cggcgtagga cgagctgccc acccctgcgt tccgccgaga tctcgtcacg
     1021 ccctgccgtc gtcaaccacg cggcgcgcca cgaagccagg tcgagcctgc tggaccgctc
     1081 catcgacgcg agcaagtccc ccatgtcggc attgccgaag gcatgcgcct gcacatggtg
     1141 cctcagcccc tgcacgaacg ccgcgtcacc gacatacgcg atgagctggc gcagtacggc
     1201 ggcacccttg gcatacgcga tcccgtcgaa gtcctgcagc gcactctgcg cgtccagggc
     1261 cagccccccg gcgaccgggt gcgtcgacgg cgcccgctcc gcggcatacc cccaggcctt
     1321 gcggatgatc gtgctgccga cccaggcgtc tgtatgccgc gtcgcctcga cgagcgcgcg
     1381 gtgcgagagg tactcggcga acgactcgtt gagccacagg tcgtcccacc agcgcatcgt
     1441 cacgaggtcg ccgaaccaca tgtgcgccat ctcgtgagcg atggtgttgt cgcgggtctg
     1501 gatctcgtcg cccgtcgccg ccccgcggaa gaggtactgg tcgcggatcg tcacgcaagc
     1561 cgggttctcc atcgccccgg cgttgaactc
//
LOCUS       DATAIX010000508         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_250410, whole genome shotgun sequence.
ACCESSION   DATAIX010000508 DATAIX010000000
VERSION     DATAIX010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250410"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VHM65_07605"
     CDS             complement(<1..461)
                     /locus_tag="VHM65_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEX2705607.1"
                     /translation="MSGLVLLAHGSPDPRHARDVDVLAERVRRARPERSVSTAYLTHD
                     APSPRDVRIGRDGSVLIVPVLLARARHMQVDVPEAARLIAARTTGLVTAAGALGPHPL
                     VLAGVGQLLRRGGVQPGPSTAVVLCAAGSTDGAASAMLGATLRDSPPPQGWG"
     gene            complement(458..988)
                     /locus_tag="VHM65_07610"
     CDS             complement(458..988)
                     /locus_tag="VHM65_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2705608.1"
                     /translation="MTGPGYPLTLDLRGRRVVVVGGGAVGTRRAMALAEAGAMVEVVA
                     PRLSGQLAALAEQGQVVWRPRGYLAGDLTEPRRAWLVQAATDDPAVNAAVGAEAQAAG
                     IWCVRADDVRASMAWTPAVVRGAEDTDAHGIVLAVTADGDPGRSVAVRNALRDALHSG
                     ALPVRRVRTRTREALR"
     gene            complement(1002..1775)
                     /locus_tag="VHM65_07615"
     CDS             complement(1002..1775)
                     /locus_tag="VHM65_07615"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX2705609.1"
                     /translation="MTVVDRPVEPLRPLYDRTPDSIRLLAGRASLDFQSREVTATTYP
                     SRVQLAREVLEWAGHAFGDKLTVASSMGDTVLIHLAGVAIPGVDVFFIDTGYHFAETL
                     GTRDAYGVMLPLLIRTIAPRQSVAEQDSEHGSRLFEREPDRCCALRKVEPLERALVDR
                     QAWVSGLRRADAVTRTGVELVGWDVKRAIAKLNPIAAWTDEDVERYTAENAVFENPLK
                     QIGYASIGCAPCTRPVRPGEDPRAGRWAGRAKVECGLHA"
     gene            complement(1772..1957)
                     /locus_tag="VHM65_07620"
     CDS             complement(1772..1957)
                     /locus_tag="VHM65_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705610.1"
                     /translation="MSARAAPIYHCPFCGEQDLRPSEESPEAWACRSCARVFTVGLMR
                     LDETLIPGRVASPGGDR"
     gene            complement(1954..>2868)
                     /locus_tag="VHM65_07625"
     CDS             complement(1954..>2868)
                     /locus_tag="VHM65_07625"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX2705611.1"
                     /translation="MLAQRLGVWVGLDEVPEVWAGVISIFRDYGYRRLRTRARLKFLV
                     ADWGAERFRAVLEGEYLGRRLADGPPPEQPEGRRRDHVGVHAQRDGRLWVGVAPVAGR
                     VSGSTLAALADLAQTHGSGRVRLTPHQKILVLDVEPGRVEALVARLDALGLPSAPSEF
                     RRGVMACTGIEYCKLALVETKARAAQTVTALEAALPGFDASLSIHVNGCPNSCARFQV
                     ADIGLKGMIMADPADPAGPSVEGFQVHLGGSLGEDAALARKTRSLRVTAEELPTYVER
                     VATRYTEQREDGESFARWARRADEEALR"
BASE COUNT          377 a         1057 c         1084 g          350 t
ORIGIN      
        1 ccccagccct gcggtggcgg gctgtcccgc agcgtcgcgc ccagcatggc cgacgccgcc
       61 ccgtcggtcg acccggcggc gcacagcacg accgcggtcg acgggccggg ctgcacccca
      121 ccgcggcgca ggagctggcc gacgccggcc agcacgagcg gatggggacc gagcgcccct
      181 gcggccgtca ccaggccggt ggtccgtgcg gcgatgagcc gagccgcttc ggggacgtcg
      241 acctgcatat gacgggcccg cgccagcagg acgggcacga tgagcacaga tccgtcccgc
      301 ccgatgcgaa cgtcccgcgg gctgggtgcg tcgtgggtca ggtatgccgt gctgacgctg
      361 cgctccgggc gcgcccggcg gacccgctcg gcgaggacgt cgacatcgcg ggcgtgccgc
      421 gggtccggtg agccgtgcgc gagcaggacg aggccgctca ccgcagcgcc tcccgcgtcc
      481 gggtcctgac ccggcgcacc ggcagcgcgc ccgagtgcag cgcgtcccgc agggcgttgc
      541 ggacggcgac cgagcgaccc gggtcaccgt cggccgtgac ggccaggacg atcccgtggg
      601 cgtcggtgtc ctcggccccg cggacgaccg caggggtcca cgccatcgac gcgcgcacgt
      661 cgtcggcacg gacgcaccag atgccggccg cctgcgcctc ggcgcccacg gccgcgttga
      721 cggccgggtc gtcggtcgcc gcctggacga gccaggcccg ccgcggctcg gtgaggtcgc
      781 ccgcgaggta cccgcgcggg cgccatacca cctggccctg ctcggccagc gcggcgagct
      841 gcccgctgag ccgcggtgcg acgacctcga ccatggctcc ggcctcggcc agcgccatgg
      901 cacgccgggt cccgacggcg ccaccgccca cgacgacgac gcgccgtccg cgcaggtcga
      961 gggtgagcgg gtacccaggc ccggtcatgc gtgcagcccg gtcatgcgtg cagcccgcac
     1021 tcgaccttgg cccggccggc ccagcgtccg gcccgcgggt cctcgccggg acggaccggg
     1081 cgggtgcagg gcgcgcagcc gatcgaggca tagccgatct gcttgagcgg gttctcgaac
     1141 accgcgttct cggcggtgta gcgctcgacg tcctcgtcgg tccacgcggc gatcgggttg
     1201 agcttggcga tggcgcgctt gacgtcccag ccgacgagct cgacaccggt ccgggtcacc
     1261 gcgtcggcgc ggcgcaggcc actgacccag gcctggcggt cgacgagcgc ccgctcgagc
     1321 ggctcgacct tgcgcagcgc gcagcaccgg tcgggctcgc gctcgaagag gcgcgagccg
     1381 tgctcgctgt cctgctcggc cacgctctgg cgcggggcga tcgtgcggat cagcaggggc
     1441 agcatgacgc cgtaggcatc gcgggtcccg agcgtctcgg cgaagtggta gcccgtgtcg
     1501 atgaagaaca cgtcaacccc ggggatcgcg actcccgcga ggtggatcag gacggtgtca
     1561 cccatcgagg acgcgacggt gagcttgtcg ccgaaggcat gccccgccca ttcgagcacc
     1621 tcgcgggcga gctggacccg gctcgggtat gtcgtcgccg tcacctcgcg cgactggaag
     1681 tccaggctcg cccgcccggc aagcagcctg atgctgtccg gcgtgcggtc gtagagcggg
     1741 cgcaggggct cgacgggccg gtcgaccacg gtcaccggtc acctcccggc gaagcgaccc
     1801 gcccggggat cagtgtctcg tcgagccgca tgagaccgac cgtgaagacc cgggcgcagg
     1861 accggcaggc ccacgcctcc ggcgactcct cgctcggacg caggtcctgc tcgccgcaga
     1921 acgggcagtg gtagatgggc gcggcgcggg cactcatcgc agcgcctcct cgtcggcccg
     1981 gcgggcccag cgggcgaacg actcgccgtc ctcacgctgc tcggtgtagc gggtggcgac
     2041 ccgctcgaca tacgtgggga gctcctcggc cgtgacccgc agggagcggg tcttgcgggc
     2101 gagcgcggcg tcctctccga ggctgccacc gaggtggacc tggaagccct cgacactggg
     2161 gccggccggg tcggccgggt ccgccatgat catgcccttg agcccgatgt cggcgacctg
     2221 gaagcgcgcg cacgagttgg ggcagccgtt gacgtggatc gacagggatg cgtcgaagcc
     2281 cggcagcgcg gcctcgaggg cggtgacggt ctgggccgcc cgggccttgg tctcgacgag
     2341 ggcgagcttg cagtactcga tgccggtgca ggccatgacg ccacggcgga actcgctcgg
     2401 cgcgctcggc aggcccaggg catccagacg ggcgacaagg gcctcgacgc gtccgggctc
     2461 gacgtcgagg acgaggatct tctggtgggg cgtcagacgg acccgtcccg atccatgcgt
     2521 ctgcgcgagg tcggccaggg ccgcgagggt gctcccgctg acccgcccgg cgaccggggc
     2581 gaccccgacc cacaagcggc cgtcgcgctg ggcgtggaca ccgacgtggt cgcgccggcg
     2641 gccctcgggc tgctcgggtg gcggtccgtc ggcgaggcgc cggccaaggt actcgccctc
     2701 gaggaccgca cggaagcgct cggcgcccca gtcggcgacg aggaacttca gcctcgcccg
     2761 ggtgcgcagc ctccggtagc cgtagtcgcg gaagatcgag atgaccccgg cccagacctc
     2821 gggtacctcg tcgaggccga cccagacccc gaggcgctgg gccagcat
//
LOCUS       DATAIX010000509         3595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_57861, whole genome shotgun sequence.
ACCESSION   DATAIX010000509 DATAIX010000000
VERSION     DATAIX010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57861"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VHM65_07630"
     CDS             complement(<1..182)
                     /locus_tag="VHM65_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705612.1"
                     /translation="MTLSTSQRELAVGIGAAGLATADMVLNIGPQHPATHGVLRLRIV
                     LDGERIVSAEPVIGYM"
     gene            328..1509
                     /locus_tag="VHM65_07635"
     CDS             328..1509
                     /locus_tag="VHM65_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX2705613.1"
                     /translation="MTFDAAELLDLADPAVVADPYPHFRALRGRAPVAWHSGLGLYVA
                     TSHAACSQLLRDRNLGRIVAPRLPEQEWATFNWLHADSILESEPPKHTRLRRLVAGAF
                     GRGHVARMAPRIDLLARRLLDGCAERLSEHGSFDVLAGLAEPLPVLVIAELLDWPEAD
                     RHLLRPWSQAIVAMYEVTRTREQEAEARTASAEFAEYVEQLAAERARRPGADLLSDLV
                     LARDGSDRLSARELVATAVLLLNAGHEASVNGFGNGLASLLASGRAWGDPGRLVEEML
                     RHDSPLHLFERTATVDTQVGDVLVARGEKVAALLGAANRDPEVFAEPDEFRPGRDPNP
                     HLAFGAGLHFCVGAPLARLELSISIRRLAERVGPVRVVEAVRRPTFVLRGYERLVLAP
                     A"
     gene            complement(1479..2477)
                     /locus_tag="VHM65_07640"
     CDS             complement(1479..2477)
                     /locus_tag="VHM65_07640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX2705614.1"
                     /translation="MIFLPWADAWQDALYGESGFYRGDGPGAHFTTATHGGFGAALAD
                     ALVHLSRREGLTRIVDLGAGRGELLGHLHRADPTLALTGVDIVARPEGLPGPVEWLTT
                     PGGQALPSGLRGLTGTLVVAHEWLDVVPCPVAEVDGSSTLRVVLVDPATGEETLGEAL
                     AGADLLWAHRFWAVPAARGRAGAGCRIEIGRPRDAVWDELLTRIDSGLALAVDYGHVA
                     GSRPPEGTLTAYRHGCQVAPVPDGRCDITAHVAVDSLTHDELVQQRAALQDLGLTGHR
                     PADELAHSDPAAYLAALTRASHEAALIARGGFGDFWWVMARRALPTGQAGARTRRS"
     gene            2564..3505
                     /locus_tag="VHM65_07645"
     CDS             2564..3505
                     /locus_tag="VHM65_07645"
                     /inference="COORDINATES: protein motif:HMM:NF022187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEX2705615.1"
                     /translation="MTDPAQRPARLTVGVVGAGKVGSVLGAGLARAGHRVTGACAVSQ
                     ASRERAAALLPGVPIGPAPEVVAAADLALLAVPDDTLGPLVAGLAAAGAFQPGQVVLH
                     TSGRHGIAILEPAMASQVLPLALHPAMTFTGTVSDLGRLPECCFGITTLIALRPLGEA
                     LAIELGGEPVWIEEEDRVLYHAALAHGSNHLVTLAAQAMEILSVAGIEAPDRVLGPLL
                     RAALDNVLRMGDSALTGPVARGDAGTVAEHLRLLAPLGTDVLPTYRALARATAERAVV
                     SGRLGVDAAAALLDVLAEPAPPKPPDPSDDPTDQEPT"
BASE COUNT          442 a         1339 c         1339 g          475 t
ORIGIN      
        1 tgcatgtagc cgatgaccgg ctccgcgctc acgatgcgct caccgtcgag gacgatgcgc
       61 aaccggagca cgccgtgcgt cgcggggtgc tgcgggccga tgttgaggac catgtcggct
      121 gtggcgagcc cggccgcacc gatgcctacg gcgagctcgc gctgggaggt gctgagcgtc
      181 atacggatga gtctgccgta tgacgcgccg agcgccacct accgaaggtc acgggacgtg
      241 ccggagccgg cccgaacggg gtgcgtcggc ccgggaaccg tgcgacctgg cccgggaggc
      301 agctcgggta gggcaggctg ggcgtccatg acgttcgacg ccgccgagct gctcgacctg
      361 gccgacccgg cggttgtcgc cgacccgtac ccgcacttca gggcgctgcg gggccgcgcg
      421 cccgtggcct ggcacagcgg gctcgggctg tatgtcgcga cgagccacgc cgcgtgcagc
      481 cagctcctgc gggaccgcaa cctcgggcgc atcgtcgccc cccggctccc ggagcaggag
      541 tgggcgacgt tcaactggct ccatgccgac tcgatcctcg aaagcgagcc ccccaagcac
      601 acgcggctgc gtcgcctcgt cgccggtgcc ttcggccgcg ggcacgtcgc ccggatggcg
      661 ccacggatcg acctgctcgc gcgtcgcctc ctcgacgggt gcgccgagcg gctgagtgag
      721 catggctcct tcgatgtcct cgccggcctg gccgagcccc tgccggtgct cgtcatcgcc
      781 gagctcctcg actggccgga ggcggaccgc cacctgctgc ggccgtggtc ccaggcgatc
      841 gtcgcgatgt acgaggtcac ccgtacccgg gagcaggagg ccgaggcgcg gacggccagc
      901 gccgagttcg ccgagtatgt cgagcagctc gccgccgagc gtgccaggcg ccccggcgcc
      961 gacctgctca gcgacctcgt cctggcccgg gacgggtcag accggctgtc ggcccgagag
     1021 ctcgtcgcca ccgccgtgct cctgctcaac gccggccacg aggccagcgt caacggtttc
     1081 ggcaacgggc tggcgtcgct gctggccagc ggccgggcat ggggcgaccc cggccggctc
     1141 gtcgaggaga tgctccggca cgactccccg ctgcacctct tcgagcggac ggccacggta
     1201 gacacgcagg tcggggacgt tctcgtggcc aggggtgaga aggtcgccgc gttgctcggg
     1261 gcggccaacc gtgacccgga ggtctttgcc gagccggacg agttccgccc aggccgcgac
     1321 ccgaacccgc atctggcctt cggcgccggg ctgcacttct gtgtcggggc accgctggcc
     1381 cggctggagc tgtcgatctc catccgccgc ctcgccgagc gcgtcggtcc ggtccgggtg
     1441 gtcgaggccg tgcggcggcc gaccttcgtc ctgcggggct acgagcgcct cgtcctcgcc
     1501 ccggcctgac ccgtgggcag ggcccggcgg gccatcaccc accagaagtc gccgaacccg
     1561 ccgcgggcga tgagcgccgc ctcgtggctg gcgcgggtga gggcggcgag ataggcggcc
     1621 gggtccgagt gcgccagctc gtcggcgggg cggtggccgg tgaggcccag gtcctgcagc
     1681 gccgcacgct gctggacgag ctcgtcgtgg gtgaggctgt cgacggccac gtgcgccgtg
     1741 atgtcgcatc gcccgtccgg aaccggcgcg acctgacagc cgtgccggta tgccgtgagc
     1801 gtcccctcgg gcgggcggga cccggcgacg tggccgtagt cgacggcgag ggccaggccg
     1861 gagtcgatcc gggtgaggag ctcgtcccac accgcgtcgc gggggcggcc gatctcgatg
     1921 cggcacccgg ccccggcccg gccgcgggcg gctgggacgg cccagaaccg gtgtgcccag
     1981 agcaggtctg cacccgcaag ggcctcgccc agggtctcct cgccggttgc cgggtcgacg
     2041 aggacgaccc gcagcgtgct cgagccgtcg acctcggcga ccgggcatgg gacgacatcg
     2101 agccactcgt gcgcaacgac gagggtgccg gtgaggccgc gcaggcctga cggcagggct
     2161 tggcctccgg gtgtggtgag ccactcgacc gggcccggca ggccttcggg gcgcgccacg
     2221 atgtcgaccc cggtgagcgc cagcgtcggg tcggcccggt gcaggtgccc gagcagctcg
     2281 ccgcggccgg cgccgaggtc gacgatgcgg gtgagcccct cgcggcggga caggtgcacc
     2341 agggcgtcgg ccagcgcggc gccgaaccct ccgtgtgtcg cggtcgtgaa atgggcaccg
     2401 gggccgtctc cccggtagaa accggactcg ccgtacaggg cgtcctgcca ggcatcggcc
     2461 cagggaagga agatcacccg ggtaatctac cgagcgcagc ccggccgggt cggtcgcgtt
     2521 cacgcgtggc tgcggcttcg accggcgcga taggttcgtg ctcgtgactg accccgcgca
     2581 acgccccgcg cgtctcacgg tcggcgtcgt cggagccggc aaggtcggct ccgtcctcgg
     2641 tgcgggcctg gcccgggccg ggcaccgggt caccggagcc tgcgcggtga gccaggcgtc
     2701 acgggagcgg gccgccgcac tgcttcccgg cgtcccgatc ggccccgctc cggaagtcgt
     2761 cgccgccgcc gacctggccc tgctggcggt gcccgacgac acgctcggtc cgctggtagc
     2821 cggacttgcc gccgcaggcg ccttccagcc ggggcaggtc gtgctgcaca cctcgggccg
     2881 ccacgggatc gccatcctcg agccggcgat ggccagccag gtcctgccct tggcgctgca
     2941 cccggccatg acgttcacgg gcacggtgag tgacctgggg cggctgccgg agtgctgttt
     3001 cgggatcacg acactcatcg ccctgcgccc gctcggtgag gccctcgcca tcgagctcgg
     3061 cggggagccg gtctggatcg aggaggagga ccgcgttctc taccacgcgg ccctcgcgca
     3121 cggctccaac cacctcgtca ccctggccgc ccaggcgatg gagatcctct ccgtggccgg
     3181 gatcgaggcc cccgaccggg tgctcggccc gctgcttcgc gccgccctcg acaacgtcct
     3241 gcgcatgggc gactccgcac tgaccggacc ggtcgcccgc ggggatgcgg gcaccgtcgc
     3301 cgagcaccta cgcctgctgg cccccctcgg gaccgatgtg ctgccgacct accgcgccct
     3361 ggcccgggca acggccgagc gcgccgtcgt ctccggtcgc ctcggcgtcg acgccgcggc
     3421 ggctctgctg gacgtgctcg ccgaaccggc accgccgaag ccacctgacc cctccgatga
     3481 cccgaccgac caggagccga catgaccggg cccaccacga gcgagggcgt cgaggccggg
     3541 cgggcgtccc gcagcgcggg ctcggccgtc gcgcgcaccc gggacgagct gcggg
//
LOCUS       DATAIX010000510         2984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_177041, whole genome shotgun sequence.
ACCESSION   DATAIX010000510 DATAIX010000000
VERSION     DATAIX010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177041"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..2045)
                     /locus_tag="VHM65_07650"
     CDS             complement(78..2045)
                     /locus_tag="VHM65_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX2705616.1"
                     /translation="MSTHASAHPAPGSVAAQAGSPSYRYGGRLAPEGTAVDPILVEIV
                     QGSLASVEMEVETAIGRTSRSPMIRDAHDFRAGIHDRHLRKLTGRSYSALVHPVARDY
                     PLETMREGDVFFHNDVYASEGGIGHLPDLCVTVPVFHEGMVVAFVQAFGHHDDIGGAC
                     PGSMPSGATSVFEEGLMVPPIRLWDAGVPNESALKIMTRNSRMPDSLAADLDAECSAC
                     LMGARRLADLFGRYGRETVEAAFDAILEQTTQTYRREILSQIPDGTYTWEDYAEHDGV
                     DEPRLHTQRITLTKAPTGGPGPGDGGPKLILDFTGTAPQAKGPINHCGDYADGNFLAK
                     WLAPILRNLAETPERMAELDVNEGVVPLIEMRFPEKGTLLTPIFPAPTNARTFVILRL
                     LGVLAGVVAKAVDGKMPADQETIRYTGVYGKDRDGNDYLMREVLGGGSGGRYYADGED
                     TIHVVPDSRNLPTEFTESRFPFIVERLGLAVDSGGAGRYRGGLGYEKHIRMLKDAHFM
                     SIADRSILACWGVKGGKAGRPFQVTIGLGSPAERVVDALADDEPVSAGEVIRIRTTGG
                     GGWGDPLDRPYDEVARDVRWGKVSFEGARADYGVVATGPYDDPVVDVPASDARRASIR
                     AARPEVEPFFDRGPGYARLSGGATSAACDWL"
     gene            complement(2042..>2984)
                     /locus_tag="VHM65_07655"
     CDS             complement(2042..>2984)
                     /locus_tag="VHM65_07655"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX2705617.1"
                     /translation="LLSGEIPLDAAAAAAGIDEIAGRLGLSRDECAAGILEISAWNQA
                     NALRQVSVKRGLDVRDFMLTTFGGSGSLLACRLVDILDLAGVVVPTNPGTVSAFGLLT
                     VDVKNDYVQTAVAKHAHLDLAAMTRTFDELTGRAGHALDLEGFARERHQYSRTADLRY
                     FGQAFEVRVGVPAGALDQGLADQVGDAFHEEHRALYGYDFRHDPRQQVEWVNLRVTGV
                     GPITRPELREIAAANRADPDDTAERARRGTRPVCFDTAGGYVETPLYWRADLRSGDAL
                     GGPVILEEYGSTVPVHPGFAVRVDAFGNLVITKEATP"
BASE COUNT          419 a         1000 c         1128 g          437 t
ORIGIN      
        1 gtctcccccg acgccgactt cgagggtctg gcgatgctgc gccggatcga cataccggac
       61 gccttgacgg gtcggtctta cagccagtcg caggccgccg acgtggcacc tccggacagg
      121 cgggcgtagc cggggccacg gtcgaagaac ggctccacct ccgggcgggc cgcgcgtatc
      181 gaggcgcgcc gcgcgtcgga ggcgggaacg tcgacgaccg ggtcgtcata cggccccgtt
      241 gccacgacgc cgtagtcggc ccgcgctccc tcgaacgaca ccttgcccca ccgcacgtcc
      301 cgcgcgacct cgtcatacgg gcggtcgagg gggtcgcccc acccgccgcc gccggtggtg
      361 cggatgcgga tcacctctcc ggcggacacc ggctcgtcgt cggcgagggc gtcgacgacg
      421 cgctcggccg ggctaccgag gccgatcgtg acctggaacg gccgcccggc cttgccgccc
      481 ttgacccccc agcaggcgag gatcgagcga tcggcgatcg acatgaagtg ggcgtccttg
      541 agcatccgga tgtgcttctc gtagccgaga ccgccgcggt agcgcccggc cccgccggag
      601 tcgaccgcga ggccgagccg ctcgacgatg aacgggaagc ggctctcggt gaactcggtc
      661 ggcaggttgc gcgagtcggg gacgacgtgg atcgtgtcct cgccgtcggc gtagtagcgt
      721 ccgccggagc caccgccgag cacctcacgc atgaggtagt cgttgccgtc gcggtccttg
      781 ccgtagacac cggtgtagcg gatcgtctcc tggtcggcgg gcatcttgcc gtcgacggcc
      841 ttggcgacga cgccggcgag caccccgagc aggcgcagga tgacgaaggt ccgtgcgttg
      901 gtgggcgccg ggaagatcgg cgtgagcagg gtgcccttct ccgggaagcg catctcgatg
      961 aggggcacga cgccttcgtt gacgtcgagc tcggccatcc gctccggggt ctcggcaagg
     1021 ttgcgcagga tcggcgcgag ccacttggcg aggaagttgc cgtcggcata gtcgccgcag
     1081 tggttgatcg gccccttggc ctggggcgcg gtgccggtga agtcgaggat gagcttgggg
     1141 ccgccgtcgc cggggcccgg accgccggtg ggcgccttgg tcaacgtgat ccgctgggtg
     1201 tgcaggcgag gctcgtcgac gccgtcgtgc tcggcgtagt cctcccaggt ataggtgccg
     1261 tccgggatct gggacaggat ctcgcggcgg taggtctgcg tcgtctgctc gaggatggcg
     1321 tcgaacgccg cctcgaccgt ctcgcggcca tagcggccga acaggtcggc aagccgtcgc
     1381 gcccccatga ggcaggcgga gcactcggcg tcgaggtcgg cggcgaggct gtcgggcatc
     1441 cgggagttgc gggtcatgat cttgagggcc gactcgttcg gcacgccggc gtcccagagc
     1501 cggatcgggg ggaccatgag cccctcttcg aagacgctcg tcgccccgga gggcatcgac
     1561 ccggggcacg cgcccccgat gtcgtcgtgg tgcccgaacg cctggacgaa cgcgacgacc
     1621 atgccctcgt ggaagaccgg cacggtgaca cacaggtcgg gcagatgccc gataccgccc
     1681 tcggatgcgt agacgtcgtt gtggaagaac acgtcgccct cgcgcatcgt ctccagcggg
     1741 tagtcccgcg cgaccgggtg gacgagcgcc gagtaggagc gcccggtcag cttgcgcaga
     1801 tgacggtcgt ggatgccggc ccggaagtcg tgcgcgtcgc ggatcatcgg ggagcgcgag
     1861 gtgcgcccga tggcggtctc gacctccatc tcgacgctgg ccagcgaccc ctggacgatc
     1921 tcgacgagga tcgggtcgac ggccgttccc tcgggagcca gccgcccgcc ataccggtat
     1981 gacggtgagc cagcctgtgc cgcaacggaa cccggcgcgg ggtgggcgct tgcgtgggtg
     2041 ctcacggggt cgcctccttg gtgatgacga ggttgccgaa ggcgtcgacc cggacggcga
     2101 agcccgggtg gacggggacg gtcgagccgt actcctcgag gatgaccgga ccgcccaagg
     2161 cgtcgcccga gcgcaggtcc gcccgccagt agagcggggt ctcgacgtag ccgccggcgg
     2221 tgtcgaagca caccggacgg gtgcctcggc gggcccgctc ggcggtgtcg tccgggtcgg
     2281 cacggttggc cgcagcgatc tcgcgcagct cgggccgggt gatcgggccg acaccggtga
     2341 cgcgcaggtt gacccactcg acctgctggc gcgggtcgtg acggaagtcg tagccgtaga
     2401 gagcgcggtg ctcctcgtgg aaggcgtccc cgacctggtc ggccagtccc tggtcgagcg
     2461 cgcccgcggg cacgccgacg cggacctcga aggcctgccc gaagtagcgc aggtcggccg
     2521 tccgcgagta ctggtggcgc tcgcgcgcga acccttcgag gtcgagggca tggccggccc
     2581 ggccggtgag ctcgtcgaag gtccgcgtca tcgcggcgag gtcgagatgg gcgtgcttgg
     2641 cgacggccgt ctggacgtag tcgttcttga cgtcgacggt gagcaggccg aacgccgaca
     2701 cggtgccggg gttcgtcggc acgacgaccc cggccaggtc gaggatgtcg acgaggcggc
     2761 aggcgagcag ggacccggag cctccgaagg tcgtcagcat gaagtcgcgc acgtccagac
     2821 cccgcttgac cgacacctga cgcagcgcgt tggcctggtt ccaggcggag atctcgagga
     2881 tgccggctgc gcactcgtcg cggctcaggc cgagccgccc tgcgatctcg tcgatcccgg
     2941 cggcggctgc ggccgcatcg agggggatct cgccgctgag cagg
//
LOCUS       DATAIX010000511         4121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_57888, whole genome shotgun sequence.
ACCESSION   DATAIX010000511 DATAIX010000000
VERSION     DATAIX010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57888"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..795)
                     /locus_tag="VHM65_07660"
     CDS             complement(52..795)
                     /locus_tag="VHM65_07660"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008693.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX2705618.1"
                     /translation="MTAPLFILDAHLWEDLAPGSEVTIDGSEGRHASAVTRVRPGEMV
                     YVTDGAGRRALAEVTHVVRGTIVAKVVEIETVAEPSLRLVLVQALAKGGRDEQAVESA
                     TELGVDVVVPWQADRSVARWRPERAAKAREKWVQVTRAATKQSRRSRIPEVTPLATTT
                     DLVARVVGAPLALVLEAGAAETLAGVTLPTEGDVTVIVGPEGGISAQELEALVRAGAR
                     PVRLGAEIMRTSSAGPAALAVICAQTRWR"
     gene            complement(853..1974)
                     /gene="dnaJ"
                     /locus_tag="VHM65_07665"
     CDS             complement(853..1974)
                     /gene="dnaJ"
                     /locus_tag="VHM65_07665"
                     /inference="COORDINATES: protein motif:HMM:NF010871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX2705619.1"
                     /translation="MNDYYGALGVTRDASPEEIKRAYRQLARKLHPDVNSGHEAEEDF
                     KKVSQAYDVLGDADKRRAYDMGADPYSGAAAGGFGAGFSFSDVMDAFFGAGSTGRGPR
                     SRVQRGQDALVRLSIELSQAVFGSEEELALDTALACSSCRGDGRQPGTGTRVCQICNG
                     AGEVQHIQNHFLGQVRTARPCQACHGFGSVVTHPCFECSGEGRVRTHRSLRLKIPAGV
                     DTGTRIQLAGEGEVGPGAGPAGDLYVELTVRPHETYTRRGDDLHCTIELPMTAAALGT
                     ALWLRTFDGERELVVPAGTQSGDVLTMRQLGVTHLRGTGRGDLMVHLGVLTPTRITAE
                     QEDLLRQLAALRGEERPEGKLTPANPGLLGKLRDAFKAK"
     gene            complement(2043..3074)
                     /gene="hrcA"
                     /locus_tag="VHM65_07670"
     CDS             complement(2043..3074)
                     /gene="hrcA"
                     /locus_tag="VHM65_07670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX2705620.1"
                     /translation="MSELRRNAVLRAIVEDYVQTSEPVGSKALLERHHLGVSAATIRN
                     DMALLEEEGLIHAPHTSAGRVPTDAGYRLFVDRLSSVKPLSRPERSAIHTFLEGAVDL
                     DDVVDRTVHLLASLTRQVAVMQYPSLNRSSVRHVELVPLGGPRVMIVLIVNSGRVEQR
                     VVDLGQGLDRAPGEPLLRRLRARINAETAGRPFTEAADRLQSLADEFGPGDRDGVRAV
                     VRALDEALVEQREERVVLAGTANLARLVHDFPLSIGPVLEALEEHVVLLKLIQTAGLD
                     ADAVAVTIGRENPHEGLASTSVVSTGYGSGNEVMAGLGVLGPTRMDYPVTMAAVRAVA
                     RYVSEILGP"
     gene            3222..4115
                     /locus_tag="VHM65_07675"
     CDS             3222..4115
                     /locus_tag="VHM65_07675"
                     /inference="COORDINATES: protein motif:HMM:NF022740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3097 family protein"
                     /protein_id="HEX2705621.1"
                     /translation="MTGTGSGGDRYGADVLGGDRRAGPRGRARDVAAEPGVVVEEAET
                     RWCGAVVRVEKAGGMHVVHLEDRHGRVRAFPLGPGFLLDGAPVVLTAPRAASAAALAG
                     MRAAPSRTVSGSLAVAGAPARVAVASRIYVEGRHDAELVEHVWGADLRVEGVVVEMLD
                     GVDRLEEAIRGFGPGPGRRMGVLVDHLVPGSKESRIVEAVEARLSAREHVLVVGHPYV
                     DVWQSVRPGRLGWSQWPTVPREVEWKRGICAALGWPHASQADLAQAWRRILRTVRSYA
                     DLEPTVLGRVEELLDFVTAAD"
BASE COUNT          527 a         1463 c         1537 g          594 t
ORIGIN      
        1 tgggctagcg ccggtggaag tcggcgccgg tggaagtcgg cgctggtgcg gtcagcgcca
       61 ccgggtctgc gcgcagatga cggcgagagc cgctggcccg gcgctggatg tgcgcatgat
      121 ctcggccccg agccggaccg ggcgcgcgcc cgccctcacg agggcctcga gctcctgcgc
      181 cgagatccca ccctcgggac cgacgatgac cgtgacgtcg ccctcggtcg ggagggtgac
      241 ccccgccagc gtctcggctg caccggcctc caggacgagc gcgagcgggg cgccgacgac
      301 ccgggccacg aggtccgtgg tggtggcgag cggggtcacc tcggggatgc gcgaccggcg
      361 gctctgcttg gtcgcggccc gggtgacctg aacccacttc tcgcgggcct tcgcggctcg
      421 ctcggggcgc cagcgggcga cgctgcggtc ggcctgccat ggcacgacga cgtcgactcc
      481 gagctcggtc gccgactcga cggcctgctc gtcccggcca cccttggcca gcgcctggac
      541 gagcacgagg cgcaggctcg gctcggcgac ggtctcgatc tcgaccacct tcgccacgat
      601 cgtgccgcgc acgacgtggg tcacctcggc cagggcccgg cgaccggccc cgtcggtgac
      661 gtagaccatc tccccggggc gtacccgggt gaccgccgag gcatgccggc cctcagaacc
      721 atcgatcgtc acctcgctac ccggagccag gtcctcccag aggtgagcgt cgagaatgaa
      781 caggggcgcc gtcataccca gggatcctcc cgcatgtcgt gcttcatcct ccaccccaca
      841 ggggcgacgg cactacttgg ccttgaaggc gtcacgcagt ttgccgagca gccccgggtt
      901 cgccggggtg agcttgccct cggggcgctc ctccccgcgc agcgctgcga gctgacgcag
      961 caggtcctcc tgctcggccg tgatccgcgt cggtgtcagc acgcccaggt ggaccatgag
     1021 gtcgccgcgc ccagtgcccc gcaggtgggt cacgccgagc tggcgcatgg tgaggacgtc
     1081 ccccgactgg gttcccgccg ggaccacgag ctcacgctcg ccgtcgaagg tccgcagcca
     1141 cagggcggtc ccgagagcgg cggcggtcat cgggagctcg atcgtgcagt gcaggtcgtc
     1201 gccgcggcgc gtgtaggtct cgtgcggccg gacggtgagc tcgacgtaca ggtcgccggc
     1261 gggaccggct ccgggaccca cttcgccttc gccggcgagc tggatccgcg tgcccgtgtc
     1321 gacgccggcc gggatcttga gccgtagcga gcgatgggtc cggacccggc cctcacccga
     1381 gcactcgaag cacgggtggg tgacgaccga gccgaaaccg tggcaggcct ggcaggggcg
     1441 cgcggtccgg acctggccga ggaagtggtt ctggatgtgc tggacctcac cggcgccgtt
     1501 gcagatctgg cagacccggg tgcccgtgcc cggctgacgc ccgtcgcctc ggcagctcga
     1561 gcacgccagt gccgtatcga gggccagctc ctcctcggag ccgaacacgg cctgggacag
     1621 ctcgatcgag agccgcacga gcgcgtcctg accacgctgg acccgcgagc gcggccctcg
     1681 gcccgtcgag cccgcgccga agaaggcgtc catgacgtcg gagaacgaga acccggcgcc
     1741 gaaacccccc gcagcagcgc cggagtaggg gtcggcgccc atgtcgtagg cgcgccgctt
     1801 gtccgcgtcg ccgaggacgt cgtaggcctg ggagaccttc ttgaagtcct cctcggcctc
     1861 gtggccggag ttgacgtcgg ggtgcagctt gcgggcgagc tggcgatagg cgcgcttgat
     1921 ctcctcggga ctggcgtccc gggtcacgcc gagggcgccg tagtagtcgt tcactcaggt
     1981 ccttgtcatg ggtgggtcag tcgggatgtg tgcggcgcag cccggccggg cgctcgtctc
     2041 ggtcatggcc cgaggatctc ggacacgtag cgcgccactg cccggaccgc ggccatggtc
     2101 acggggtagt ccatgcgagt ggggcccagc acgcccagcc cggccatgac ctcgttgccg
     2161 gagccgtagc cggtcgagac gaccgacgtg ctcgcgaggc cctcgtgcgg gttctcccgc
     2221 ccgatcgtca cggcgaccgc gtcggcgtcg agccccgccg tctggatcag cttgagcagg
     2281 acgacatgct cctcgagcgc ctcgagcacc ggtccgatgc tgagcgggaa gtcgtggacc
     2341 agccgggcca ggttggccgt tcccgccagc acgacgcgct cctcgcgctg ctcgaccagg
     2401 gcctcgtcga gggcccgcac gacggcccgc accccgtccc ggtcacccgg tccgaactcg
     2461 tcggcgagcg actggaggcg gtcggcggcc tcggtgaacg gccgccccgc cgtctcggcg
     2521 ttgatccgtg cgcgcagccg tcgcagcagc ggctcgccgg gagccctgtc gagcccctgg
     2581 ccgaggtcga ccacccgctg ctcgacccgg cccgagttga cgatgagcac gatcatgacg
     2641 cgcggcccgc cgagcgggac gagctcgacg tggcgcacgc tcgaccggtt gagcgagggg
     2701 tactgcatca cggcgacctg gcgggtcaac gaggcgagca ggtggaccgt ccggtcgacg
     2761 acatcgtcga ggtcgaccgc gccctcgaga aaggtgtgga tcgccgagcg ctcgggccgg
     2821 ctcagcggct tgaccgagct gagccggtcg acgaagaggc ggtagccggc gtcggtcggc
     2881 acccgcccgg ccgacgtgtg cggggcgtgg atcaggccct cctcctcgag cagggccatg
     2941 tcgttgcgga tcgtcgcggc cgacaccccg aggtggtggc gttcgagcag cgccttggat
     3001 ccgaccggct ccgaggtctg gacgtagtcc tcgacgatgg cgcgcaggac ggcgttgcgt
     3061 cgcagctcgc tcacgtccct ggtctccctt gccacgtttg gcactccggt atgtcgagtg
     3121 ccaagtctac gtcgtccggc ccggtccggg agggcgggcc ttcaggcccg tcgcggcgtt
     3181 tcgccggagc acgcacggcc gcgacggcat accgtgtcgg gatgactggg actggctccg
     3241 gaggcgatcg gtatggcgct gacgtcctcg gcggtgaccg gcgcgccggg ccgcggggcc
     3301 gggcgcgaga cgtggccgcc gagccgggcg ttgtcgtcga ggaggccgag acgcgctggt
     3361 gcggagcggt cgtccgggtc gagaaggcgg gcgggatgca cgtcgtgcat ctcgaagacc
     3421 gccacgggcg ggtgcgggcc ttcccgctcg ggccggggtt cctcctcgac ggggcgccgg
     3481 tcgtgctcac cgcgccccgt gcggcgtcgg cggcggcgct cgccgggatg cgggcggcgc
     3541 cgtctcggac ggtcagcggc tccttggcgg tggccggtgc ccccgcgagg gtcgccgtcg
     3601 cctcgcggat ctacgtcgag ggacgacacg atgccgagct cgtcgagcac gtgtggggcg
     3661 ccgacctgcg cgtcgaagga gtcgtcgtcg agatgctcga cggcgtcgac cggctcgagg
     3721 aggcgatcag ggggttcggg ccggggcccg ggcgccggat gggcgtcctg gttgaccacc
     3781 tcgtccccgg cagcaaggag tcccgcatcg tcgaggcggt cgaggctcgg ctgtccgccc
     3841 gcgagcatgt cctcgtcgtg gggcacccct atgtcgacgt gtggcagtcg gtgcgccccg
     3901 gacgcctcgg ctggtcccag tggcccacgg tccccaggga ggtcgagtgg aaacggggca
     3961 tctgcgccgc cctcggctgg ccccacgcca gccaggccga cctcgcccag gcctggcggc
     4021 ggatcctgcg caccgtccgc tcgtatgctg atcttgagcc caccgtgctc ggccgggtcg
     4081 aggagctcct cgacttcgtg accgcagcgg actgactcga c
//
LOCUS       DATAIX010000512         1865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_27593, whole genome shotgun sequence.
ACCESSION   DATAIX010000512 DATAIX010000000
VERSION     DATAIX010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27593"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..1809
                     /gene="dop"
                     /locus_tag="VHM65_07680"
     CDS             280..1809
                     /gene="dop"
                     /locus_tag="VHM65_07680"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: protein motif:HMM:TIGR03688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HEX2705622.1"
                     /translation="MTARRVMGVETEYGISVPGDPGANPMILSGHVVTAYGGSGGARA
                     SRASWDYADEAPLRDARGFEMGRGVADPSQLTDIEDPTMANLVLTNGARLYVDHAHPE
                     YSSPEVRTPRDAVRWDRAGEAIMAEASRRLARTPGFPPVNLYKNNTDGKGASYGTHEN
                     YLMRRETPFGEVVRHLTPFFVARQVICGAGRVGIGQDGGRTGFQVSQRADFFEVEVGL
                     ETTLKRPIINTRDEPHAVADLYRRLHVIIGDANQFDIASVLKLGTTSIVLAMTEDRAL
                     DGTLAVRHPVAELHAVSHDPTLRHLILLGDGRSVTALDLLWAYFECATDYLGVRGETD
                     PDTAEVMTLWEDVLDRLGRDPMLCAADLDWVAKLQLLEGYRSRDGLGWDDARLAAIDI
                     QWSDVRPDKGLVHRLRARGRVRTLVSDEAIRVAMTSPPEDTRAYFRGRCLQKYGPQVV
                     AASWDAVIFDVPGHRSLQRVPMLEPHRGTREHVGDIIDRSPDARTLLSLLDAESTGQT
                     G"
BASE COUNT          264 a          654 c          663 g          284 t
ORIGIN      
        1 agtcgcggac ctccggcatg tcctcgaggt gctcgacgat gtatgccgcg tgccggggcg
       61 agctcgccct cgcaccacgt cttgagctcg gccgagccgc gtgggcgccg ctgtcgtcgg
      121 ggtgggccag tgtctcgtgc aggtcggtca tcgcgggcat cctctcctga ggcgagcatg
      181 ctcgtcatac ccaatccttg gccttgcgct cggagcccgc cacctcgtac acccggcgtt
      241 cgcgatccgc ccggtccacg gccgccatag ggtggagcca tgacggcgcg gcgggtcatg
      301 ggggtcgaga ccgagtacgg catctcggtg ccgggtgacc cgggcgccaa ccccatgatc
      361 ctgtcggggc atgtcgtcac cgcctacggg ggcagcgggg gggcgcgcgc gagccgcgcc
      421 tcctgggact acgccgacga ggcgccgctg cgggatgcgc gggggttcga gatgggtcgc
      481 ggagtcgccg acccctcgca gctcaccgac atcgaggacc cgacgatggc caacctcgtg
      541 ctgaccaacg gggcccgtct gtatgtcgac cacgcccacc cggagtacag ctcacccgag
      601 gtgcgcaccc cccgcgacgc cgtgcgctgg gacagggctg gtgaggcgat catggccgag
      661 gcgtcccggc gcctggcccg gacaccgggc ttcccgccgg tcaacctgta caagaacaac
      721 accgacggca agggcgcctc ctacggcacc cacgagaact acctcatgcg ccgcgagacc
      781 ccgttcggtg aggtcgtgcg gcacctcacg ccgttcttcg tggcccgcca ggtcatctgt
      841 ggtgcgggcc gggtcggtat cggtcaggac gggggacgga ccgggttcca ggtgagccag
      901 cgggccgact tcttcgaggt cgaggtgggg ctcgagacga cgctcaagcg cccgatcatc
      961 aacacgcggg acgagccgca cgcggtggcg gacctgtacc ggcggcttca tgtcatcatc
     1021 ggcgacgcca accagttcga tatcgcctcg gtgctcaagc tcgggaccac ctcgatcgtc
     1081 ctggccatga ccgaggaccg tgctctggac ggcaccctcg cggtccgcca tccggtggcc
     1141 gagctccatg ccgtctcgca cgacccgaca cttcgccacc tcatcctgct cggcgacggc
     1201 cgctcggtca ccgcgctgga cctgctctgg gcttacttcg agtgcgcgac ggactatctc
     1261 ggggttcgcg gcgagacgga cccggacacc gcagaggtga tgacgctgtg ggaggacgtc
     1321 ctcgaccggc tgggccgcga tcccatgctc tgtgcggccg acctcgactg ggtggccaag
     1381 ctccagctgc tcgagggcta ccgttcgcgc gacgggctgg gctgggacga cgcccggctc
     1441 gcggcgatcg acatccagtg gagcgacgtc cggcccgaca aggggctggt ccatcggctg
     1501 cgcgcccggg gccgggtccg cacgctggtg tcggacgagg cgatccgggt ggcgatgacc
     1561 tcacctcccg aggacacgcg ggcctacttc cgggggcgct gcctgcagaa gtacggcccg
     1621 caggtcgtcg cggcctcgtg ggacgcggtg atcttcgacg tgcccggaca tcggtccctg
     1681 cagcgcgtgc cgatgctcga accgcaccgc gggacgcgcg agcacgtcgg tgacatcatc
     1741 gaccgcagcc cggacgcccg gaccctgctg tcgttgctcg atgccgagtc gaccgggcag
     1801 accgggtaga ccgggtgggc cgggactctg gcaccggtgc ggacggtacg tgcttgtgtc
     1861 agggc
//
LOCUS       DATAIX010000513         1885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_117360, whole genome shotgun sequence.
ACCESSION   DATAIX010000513 DATAIX010000000
VERSION     DATAIX010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117360"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VHM65_07685"
     CDS             complement(<1..729)
                     /locus_tag="VHM65_07685"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2705623.1"
                     /translation="MTGRSSRHPGEAGRLRAVIFDWGGTLTPWHTVDLEEQWRVFARQ
                     VHAEEEQARSLAARIFAAEAGAWLRARTEHTSSRMPEILAEAGIHEGHEALDAARAAY
                     EEFWAPHTFTDEQVRPLWEGLRGNGLRVGILSNTIWTGDYHRAVFARDGVADLIDGEV
                     YSSEIAWSKPHVEAFRAAAAAVGVTPGECAYVGDRLFEDVNGSQAAGMRAIWVPHSDL
                     PAGQQVVVEVVPDAVVPDLIAILGV"
     gene            complement(726..>1885)
                     /locus_tag="VHM65_07690"
     CDS             complement(726..>1885)
                     /locus_tag="VHM65_07690"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEX2705624.1"
                     /translation="GEELLGLRATAANGEDTYVRCPSCGFAANVEAVKVPVPVAISYG
                     DAPAMHLEQTPGSPTIATLVDCLNARYPKADGTWGAPDTLKTVVVMLVHPDGTREPLA
                     IGLPGDREIDEKRLGAQVDPATVEAFQEQDFAVNPALVKGYMGPTALGVDSESGVRFL
                     VDPRVVPGTRWVAGGNRPDVHAVDLVAGRDFTPDGTIEAAEVRDGDECPSCGHAVQTA
                     RGIEMGHIFALGRKYAEALGLKVLDQSGRLVTVIMGSYGVGVSRAVACVAEGNHDELG
                     LIWPREISPCDVHLVATGKDEAVFLAAEDLVRGLEAHGVTVLYDDRRGVSPGVKFTDS
                     ELIGVPTIVVVGRGLERGVVEVKDRRSGLRREVALESSVADVVAEVRGLSG"
BASE COUNT          269 a          697 c          661 g          258 t
ORIGIN      
        1 cacgccgagg atcgcgatga ggtcgggcac gaccgcgtcc gggacgacct cgaccacgac
       61 ctgctggccc gccgggaggt ccgagtgcgg gacccagatg gcccgcattc cggccgcctg
      121 gctgccgttg acgtcctcga agagccggtc cccgacatac gcgcactccc ccggggtgac
      181 acccaccgcg gcagcggcgg cgcggaacgc ctcgacgtgc ggcttggacc acgcgatctc
      241 gctggagtag acctcgccgt caatgagatc cgcgacaccg tcccgggcga agaccgcgcg
      301 gtggtagtcc cccgtccaga tcgtgttgga caggatcccg actcgaagtc cattgccgcg
      361 caggccctcc cacagcggac ggacctgctc gtcggtgaac gtgtggggcg cccagaactc
      421 ctcgtacgcc gcccgggcgg cgtccagcgc ctcgtggcct tcgtgaatcc ccgcctcggc
      481 gaggatctcg ggcatccggc tgctcgtgtg ctcggtccgg gcccggagcc aggcgcccgc
      541 ctccgccgcg aagatccggg cggccagtga gcgcgcctgc tcctcctcgg cgtggacctg
      601 ccgcgcgaag acccgccact gctcctcgag gtcgacggtg tgccacggcg tcagcgtgcc
      661 gccccagtcg aagatgacgg cacgcagccg ccccgcctca ccagggtgcc gagatgagcg
      721 acccgtcatc cggacaggcc gcggacctcg gccaccacgt cggcgacgga cgactcgagc
      781 gcaacctcgc gacgaagccc cgagcgccgg tccttgacct cgacgacgcc gcgctccagg
      841 ccccgcccga cgacgacgat cgtcggcacg ccgatgagct cggagtcggt gaacttcacg
      901 cccggactga cgcccctgcg gtcgtcgtag aggacggtga ctccgtgggc ttccagaccc
      961 cggaccaggt cctcggccgc gaggaagacc gcctcgtcct tgccggtcgc gacgaggtgg
     1021 acgtcgcacg gggagatctc ccgcggccag atcaggccca gctcgtcgtg gttcccctcg
     1081 gcgacacagg cgacggcccg ggacacgccg acgccgtacg agcccatgat gaccgtgacg
     1141 agcctgccgg actggtcgag caccttgagc ccgagggcct cggcgtactt gcggccgagc
     1201 gcgaagatgt ggcccatctc gatgccgcgg gccgtctgca cggcgtgccc acagctcggg
     1261 cactcgtccc cgtcgcggac ctcggcggcc tcgatcgtcc cgtcgggcgt gaagtcccgc
     1321 cccgcgacga ggtcgaccgc gtggacgtcc ggccggttgc caccggcgac ccagcgcgtg
     1381 ccggggacga cccgcgggtc gacgaggaaa cggacacccg actcgctgtc cacgcccagc
     1441 gccgtcggtc ccatgtagcc cttgaccagg gccgggttga ccgcgaagtc ctgctcctgg
     1501 aacgcctcga ccgtggccgg gtcgacctgc gccccgaggc gcttctcgtc gatctcgcgg
     1561 tcgccgggga gcccgatcgc gagcggctcg cgcgtgccgt cggggtggac gagcatgacg
     1621 acgaccgtct tgagggtgtc gggggcgccc caggtgccgt ccgccttggg gtagcgcgcg
     1681 ttgaggcagt cgacgagggt ggcgatcgtg gggctgccgg gagtctgctc gagatgcatc
     1741 gccggggcat cgccatacga gatcgccacg gggacaggca ccttgacggc ctcgacgttc
     1801 gcggcgaacc cgcagctggg gcagcggacg taggtgtcct caccgttggc cgcggtggct
     1861 cgaagtccga ggagttcctc gccac
//
LOCUS       DATAIX010000514         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_117371, whole genome shotgun sequence.
ACCESSION   DATAIX010000514 DATAIX010000000
VERSION     DATAIX010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117371"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /gene="trpC"
                     /locus_tag="VHM65_07695"
     CDS             complement(<1..732)
                     /gene="trpC"
                     /locus_tag="VHM65_07695"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001369.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX2705625.1"
                     /translation="MPSVLEQIIAGVREDLALRQIASPRADVDALALGVAPALDAYAA
                     LVGAPGVAVIAEVKRSSPSKGSLADIADPAALAVEYAAGGAAAVSVLTERRRFHGSLD
                     DLDRVREAVGIPVLRKDFTVVPYQVVEARAHGADLVLLIVAALPQPDLVSLHELTESL
                     GMTALVEVHDEEETRRAVDAGARVIGVNARNLKTLEVDRSTFARLRPLIPEGAFAIAE
                     SGVRDAADVAAYVAQGARGVLVGDVC"
     gene            complement(753..1241)
                     /locus_tag="VHM65_07700"
     CDS             complement(753..1241)
                     /locus_tag="VHM65_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705626.1"
                     /translation="MNRVDPSVRDWADALELTSALRAGQRLPELAVAQAGLEPGEPAH
                     AWLEVRVYQQSGIKLAFASSDPTTHAAAQRWAEMDTGSVVVTPARLVVLGRFGAVSFW
                     ASRVRRHWLEHDGSALEYDGATFKFVTERPVWFDVVVNAAMYRRVIDRALPEYVRRGL
                     GE"
     gene            complement(1281..>1587)
                     /locus_tag="VHM65_07705"
     CDS             complement(1281..>1587)
                     /locus_tag="VHM65_07705"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX2705627.1"
                     /translation="ASTARPAVGYEPSTPCCTAISAQPWTATRSSYMVMNVKVLRSQD
                     GQLPGFGPSFMRWLIPAAANAVCGLGIVVYLSPLFDNSGRVQGWHDKAANTLVVSTK"
BASE COUNT          249 a          541 c          587 g          210 t
ORIGIN      
        1 gcatacgtcg cccacgagga ccccccgggc tccctgggcg acgtatgccg cgacgtcggc
       61 ggcatcgcga accccggact cggcgatcgc gaaggcacct tcggggatga ggggacgcag
      121 ccgcgcgaag gtcgagcggt cgacctcgag ggtcttgagg ttgcgcgcgt tgacgccgat
      181 gacccgcgct ccggcatcga ccgcacggcg ggtctcctcc tcgtcgtgga cctcgacgag
      241 ggcggtcatc ccgagcgact cggtcagctc gtgcagcgag acgaggtccg gctgcggaag
      301 ggccgcgacg atgagcagga cgaggtcggc tccgtgggcc cgagcctcga ccacctggta
      361 gggcacgacg gtgaagtcct tgcgcaggac cggtatgccg accgcctcgc gcacacggtc
      421 gaggtcgtcg agggacccgt ggaaacgccg ccgctcggtc aggacactga cggccgcggc
      481 gccgcccgcg gcatactcga cggccagggc cgccgggtcg gcgatgtcgg cgagggaccc
      541 cttgctcggg ctggaccgct tgacctcggc gatgaccgcg acccctgggg cgcccacgag
      601 cgcggcatac gcgtcgaggg cgggggcgac cccgagagcc agcgcgtcga cgtccgcacg
      661 gggcgaggcg atctggcgca gggcgaggtc ctcacgcacg ccggcgatga tctgttcgag
      721 gacgctgggc acggcggata gcgtatagcg tgtcactcgc caagcccgcg ccggacgtac
      781 tcgggcagcg cacggtcgat gacccggcgg tacatggcgg cgttgaccac gacgtcgaac
      841 cacaccggtc gctccgtcac gaacttgaac gtcgccccgt cgtactccag ggcggacccg
      901 tcgtgttcga gccagtggcg acgcactcgg ctggcccaga agctgaccgc gccgaagcgc
      961 ccgagcacga ccaaccgggc cggtgtgacc acgaccgagc cggtgtccat ctcggcccag
     1021 cgctgcgccg cagcgtgggt cgtcgggtct gagctggcga aggcgagctt gatgccgctc
     1081 tgctggtaga ccctcacctc gagccacgca tgggcgggct ccccgggctc cagccctgcc
     1141 tgtgcgacag cgagctcggg aagccgttgc ccggcccgaa gtgccgacgt cagctccagg
     1201 gcgtcggccc agtcacgaac ggacgggtcg accctgttca cggcggtagg gctacgcctg
     1261 gcagccgggg tgagcgccgg ctacttcgtc gagacgacga gggtgttggc ggccttgtcg
     1321 tgccatccct ggacgcggcc cgagttgtcg aacagcggcg agaggtagac gacgataccg
     1381 agcccgcaca ccgcgttggc ggccgcgggg atgagccagc gcatgaacga gggtccgaac
     1441 ccggggagct ggccgtcctg ggagcgcagg accttgacat tcatgaccat gtatgacgag
     1501 cgggtggcgg tccatggctg cgccgagatc gccgtgcagc agggcgtgga cggctcgtaa
     1561 cccaccgcag gccgggcagt agacgcc
//
LOCUS       DATAIX010000515         4948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_117432, whole genome shotgun sequence.
ACCESSION   DATAIX010000515 DATAIX010000000
VERSION     DATAIX010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117432"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /gene="nuoE"
                     /locus_tag="VHM65_07710"
     CDS             complement(<1..579)
                     /gene="nuoE"
                     /locus_tag="VHM65_07710"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005721.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX2705628.1"
                     /translation="MPGTHTASGHLHVPAESKAPYDGDTLAALHEDAAAVIARYPHKR
                     SALLPLLHLVQSVDGYVTGRGIAFCAGVLDLSTAEVSGVATFYTQFKRHPNGEYTVGV
                     CTNTLCAILGGDQIWEAVSEHLGIGHDETTSDGRITLERVECNAACDYAPVVMANWEF
                     FDNQTPESTIALVEELRAGAPVAPTRGPRSVCT"
     gene            complement(579..1964)
                     /locus_tag="VHM65_07715"
     CDS             complement(579..1964)
                     /locus_tag="VHM65_07715"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX2705629.1"
                     /translation="MSKRTNETGSTRRSGPDPYATTFADEAAAEGAAVFNASGGDWDD
                     LVDEATSLGEERIVVNMGPQHPSTHGVLRLILELDGETVTETRAGIGYLHTGIEKNME
                     FRTWTQGVTFCTRMDYLTPMFQETAYCLAIEKLLGVTDEVPERASIIRVLMMELTRIS
                     SHMVCLGTGGMEMGATTVMTIGFRERERVLAIIEMVTGLRMNNAYIRPGGVAQDLPPG
                     AIDKIRDTVPLLRRGIGELEALLLENPILKGRTVGVGYLDLTGCIALGMTGPVLRSTG
                     LPHDLRKSAPYCGYEGYEFEVITRTSADAYGRLSIRLDEMRESVRIIEQAVDKLQATQ
                     GSPVMIADKKIAWPAQLALGSDGLGNSLDHIREIMGESMESLIHHFKLVTEGFRVPPG
                     QVYVAVESAKGELGAHVVSDGGTRPYRAHFRDPSFNNLQATALMCEGGQVADVIVAVA
                     SIDPVMGGVDR"
     gene            complement(1964..2668)
                     /locus_tag="VHM65_07720"
     CDS             complement(1964..2668)
                     /locus_tag="VHM65_07720"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005856.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX2705630.1"
                     /translation="MSEHDNLPASAADRAEPVFLGVRIGMFGAHDGADTTGYGGLRQP
                     ILFPAAAVRPYGGYFDDVADSLDEAMGEDAATEAIERVVVDRGEMTLFVRRAQLPVVA
                     RTLRDHPRLRFEVCTGVSGVHYPGEAGRELHAVYHFLSITHGSRRVRVEVACPDADPH
                     IPSIVRTYPANDWHERETWDMFGIIFDGHPALTRILMPDDWPGHPQRKDYPLGGIPVE
                     YKGATVPPPDQRRSYT"
     gene            complement(2665..3219)
                     /locus_tag="VHM65_07725"
     CDS             complement(2665..3219)
                     /locus_tag="VHM65_07725"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX2705631.1"
                     /translation="MGIEEKLPAGFLLSTVEGLAGYMRKSSMWPATFGLACCAIEMMA
                     VGTPDYDIARFGMERFSATPRQADLMIVAGRVSQKMAPVLRQVYDQMPNPKWVIAMGV
                     CASSGGMFNNYAIVQGVDHIVPVDIYLPGCPPRPEMLLDAIIKLHEQIQKMPLGANRV
                     AAAHAAEEAALKAIPTHQMKGLLA"
     gene            complement(3232..3594)
                     /locus_tag="VHM65_07730"
     CDS             complement(3232..3594)
                     /locus_tag="VHM65_07730"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX2705632.1"
                     /translation="MSNPYVPILFLMGLGLAFALVSVGGSLVVGPKRYNRAKMEAYEC
                     GISPTPHPAGGGRVPVKYYLTAMLFIIFDIESVFLYPFAVAFDQLGLFALVEMVLFIL
                     SVFVAYAYVWRRGGLDWD"
     gene            complement(3775..>4948)
                     /locus_tag="VHM65_07735"
     CDS             complement(3775..>4948)
                     /locus_tag="VHM65_07735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HEX2705633.1"
                     /translation="FPREKVCGDGLTPRAVRELITLGIPTPEDQGWIRNYGLRIIGGG
                     MRLQLDWPENSTFPSYGLVRPRKDFDEILARHAVRHGARLIEGTGVTGPVRDGTGRII
                     GVTARASNEAGRAVGEELTFRAPVVVAADGNSSRLSLAMGRPTRDDRPMGVAVRTYYT
                     SPRTDDDYLESWLELWTENDDGERILLPGYGWIFGVGDGTSNVGLGILNTSAAFGTVD
                     YKDVMRQWVRTMPQEWTYNDDTMVAPIRGAALPMGFNRQPHYASGLLLVGDAGGMVNP
                     FNGEGIAYAMESGRLSAEVIANAHAREGAAARERVLQSYPAVMKSELGGYYTLGRYFA
                     KAIGHPEVMRLATKYGLPRTTLMKFLLKVMANLADENGKAVSDRLIHSLSKMTPAA"
BASE COUNT          727 a         1632 c         1801 g          788 t
ORIGIN      
        1 ggtgcacacc gagcgcgggc cgcgggtggg ggccaccggc gcgcctgccc ggagctcctc
       61 gacgagggcg atcgtggact cgggtgtctg gttgtcgaag aactcccagt tggccatgac
      121 gaccggcgcg tagtcgcacg cggcgttgca ctcgacccgc tcgagggtga tcctgccgtc
      181 actcgtcgtc tcgtcgtggc cgatcccgag gtgctcgctc acggcctccc agatctggtc
      241 gccgccgagg atcgcgcaca gcgtgttggt gcacacaccg acggtgtact cgccgttggg
      301 gtggcgcttg aactgggtgt agaacgtcgc gacgccagag acctcggccg tgctcaggtc
      361 gagcacgccg gcgcagaagg cgatcccgcg gccggtgacg tagccgtcga cgctctggac
      421 gaggtgcagc agcggcagga gagccgagcg cttgtgcggg taccgtgcga tgaccgccgc
      481 ggcatcctcg tgcagcgccg cgagggtgtc gccgtcatac ggcgccttgc tctcggccgg
      541 gacatggagg tgtcctgagg cggtgtgcgt gccgggcatc agcggtctac ccctcccatg
      601 acggggtcga tcgaggcgac ggcgacgatg acgtcggcga cctggccgcc ctcgcacatc
      661 aaggccgtcg cctgcaggtt gttgaagctc gggtcgcgaa aatgcgcccg gtagggccgg
      721 gtgccgccgt cggagacgac gtgggcgccg agctcgccct tggcgctctc gaccgcgacg
      781 tagacctggc cgggcgggac gcggaagccc tcggtgacca gcttgaagtg atggatgagc
      841 gactccatcg actcgcccat gatctcgcgg atgtggtcga gggagttgcc gagcccgtcg
      901 ctgccgaggg cgagctgggc gggccaggcg atcttcttgt cggcgatcat gaccgggctg
      961 ccctgggtgg cctggagctt gtcgacggcc tgctcgatga tgcggaccga ctcgcgcatc
     1021 tcgtcgaggc ggatggacag gcgcccgtag gcgtcggccg aggtgcgggt gatgacctcg
     1081 aactcgtagc cctcgtagcc gcagtagggc gcggacttgc gcaggtcgtg cgggaggccg
     1141 gtggagcgca ggaccgggcc ggtcatgccg agggcgatgc agccggtgag gtcgaggtag
     1201 ccgacgccga ccgtgcgccc cttgaggatc gggttctcca gcaggagggc ctcgagctcg
     1261 ccgatccccc ggcgcagcaa cgggaccgtg tcgcggatct tgtcgatggc accgggcggg
     1321 aggtcctggg cgaccccgcc ggggcggatg taggcgttgt tcatccgcag gccggtgacc
     1381 atctcgatga tggcgagcac gcgctcgcgc tcccggaagc cgatcgtcat gacggtcgtc
     1441 gcccccatct ccatgccacc ggtgccgagg cagaccatgt gggaggagat ccgggtcagc
     1501 tccatcatga ggacccggat gatcgacgcc cgctcgggga cctcgtcggt gacgccgagg
     1561 agcttctcga tggccaggca gtaggccgtc tcctggaaca tcggggtgag gtagtccatg
     1621 cgggtgcaga acgtcacgcc ctgggtccag gtgcggaact ccatgttctt ctcgatgccg
     1681 gtgtgcaggt agccgatgcc cgcgcgggtc tcggtcaccg tctcgccgtc gagctcgagg
     1741 atgaggcgga gcacgccgtg cgtcgagggg tgctggggcc ccatgttgac gacgatgcgc
     1801 tcctcgccca gggatgtggc ctcgtcgacg aggtcgtccc agtcaccgcc ggacgcgttg
     1861 aagacggcgg cgccctcggc cgcggcctcg tcggcgaacg tcgtcgcgta ggggtccggg
     1921 ccgctcctgc gggtggagcc cgtctcgttc gtgcgcttgc tcatcaggtg taggacctcc
     1981 gctggtcggg cggcgggacg gtggcgccct tgtactcgac cgggatgccg ccgagggggt
     2041 agtccttgcg ctgcgggtgg ccgggccagt cgtccggcat gaggatgcgg gtcagtgccg
     2101 gatgcccgtc gaagatgatc ccgaacatgt cccacgtctc gcgctcgtgc cagtcgttgg
     2161 ccgggtaggt gcgcacgatc gacgggatgt gcgggtcggc gtcggggcag gccacctcga
     2221 cccgaacccg cctgctgccg tgggtgatcg acaggaagtg gtagaccgcg tgaagctcgc
     2281 ggccggcctc tcccgggtag tggacccccg acacgccggt gcacacctcg aagcgcagcc
     2341 tggggtggtc gcgcagcgtc cgggcgacga ccggcagctg cgcacggcgc acgaagagcg
     2401 tcatctcgcc gcggtcgacg acgacgcgct cgatggcctc ggtggccgca tcctcgccca
     2461 tcgcctcgtc cagcgagtcc gcgacgtcgt cgaagtaccc gccatacggg cgaaccgcgg
     2521 ccgcggggaa cagaatcggc tgcctcagcc cgccataccc cgtggtgtcg gcgccgtcgt
     2581 gcgcgccgaa catgccgatg cggaccccga ggaagacggg ttcggcacgg tcagcggctg
     2641 aggcgggaag gttgtcgtgc tcactcacgc cagcagcccc ttcatctggt gcgtcgggat
     2701 ggccttgagg gcggcttcct cggctgcatg cgccgccgcg acccggttgg cgccgagcgg
     2761 catcttctgg atctgctcgt gcagcttgat gatcgcgtcg agcagcatct ccggccgtgg
     2821 cgggcagccg ggcaggtaga tgtcgacggg gacgatgtgg tcgacgccct ggacgatcgc
     2881 gtagttgttg aacatcccgc ccgagctggc gcagacgccc atcgcgatga cccacttggg
     2941 gttcggcatc tggtcgtaga cctggcgcag gaccggggcc atcttctggc tcacccggcc
     3001 ggcgacgatc atgaggtcgg cctggcgcgg ggtcgccgag aagcgctcca tcccgaaccg
     3061 ggcgatgtcg tagtcagggg tcccgaccgc catcatctcg atcgcacagc aggccagtcc
     3121 gaaggtggcg ggccacatcg aggacttgcg catgtagccg gccagaccct cgaccgtcga
     3181 caggaggaac ccagcgggga gcttctcttc aatacccatg tcagcactcc cttagtccca
     3241 gtccagcccg ccgcgacgcc atacataggc gtaggcgacg aacacggaca ggatgaacag
     3301 caccatctcg acgagcgcga agagcccgag ctggtcgaac gccactgcga acgggtagag
     3361 gaacacgctt tcgatgtcga agatgatgaa gagcatggcc gtgaggtagt acttgaccgg
     3421 cacgcgccca cccccggcgg ggtgcggcgt cggcgagatg ccgcactcgt aggcctccat
     3481 cttggcccgg ttgtagcgct tgggcccgac gacgaggctc cccccgaccg agacgagggc
     3541 aaaggcgagc ccgaggccca tgaggaacag gatcggaacg tacggattgc tcatcgcgtc
     3601 tcgctcccct ttcggcttcc gatcaggctt ccgaccatgc gtgcagcgcc ggccacgccg
     3661 ggccatccca tcctaggggc gaggcctggc gatccgcggg aggccctacc cgcgctgaca
     3721 tgcgcgcggc gggccgtgcc gctgctcgga tttcgcagga ctgggctcat ggcgtcaggc
     3781 cgcgggggtc atcttcgaca gcgagtggat gagccggtcg gacacggcct tgccgttctc
     3841 gtcggcgagg ttggccatga ccttgagcag gaacttcatg agcgtcgtgc gcggcagccc
     3901 gtacttcgtc gcgaggcgca tgacctcggg gtggccgatc gccttggcga agtagcggcc
     3961 cagggtgtag tagccgccga gctcggactt catcaccgcc gggtaggact gcaggacccg
     4021 ctcacgggcc gccgcaccct cgcgggcgtg ggcgttggcg atcacctccg ccgaaagccg
     4081 tccggactcc atcgcgtagg cgatgccctc gccgttgaac gggttgacca tgccgccggc
     4141 gtcgccgacg aggagcagcc cgctggcgta gtgcggctgc cggttgaacc ccatcggcag
     4201 cgcggcaccc cggatcggcg cgaccatcgt gtcgtcgttg taggtccact cctggggcat
     4261 ggtgcgcacc cactggcgca tgacgtcctt gtagtcgacg gtgccgaacg ccgcgctcgt
     4321 gttgaggatg ccgagcccga cgttggaggt cccgtcgccc acgccgaaga tccagccata
     4381 gcccggcagc aggatgcgct cgccgtcgtc gttctcggtc cacagctcga gccacgactc
     4441 gaggtagtcg tcgtcggtgc gcgggctcgt gtagtaggta cgcaccgcca cccccatcgg
     4501 ccggtcgtcg cgcgtgggcc ggcccatcgc gagggaaagc cgcgaggagt tgccgtcggc
     4561 cgcgacgacg accggcgccc ggaaggtcag ctcctcgccg acggcgcggc cggcctcgtt
     4621 gctggcccgc gcggtgacgc cgatgatgcg cccggttccg tcgcggaccg ggccggtgac
     4681 accggtgccc tcgatgagcc gggcgccgtg ccggacggcg tgccgggcca ggatctcgtc
     4741 gaagtccttg cggggacgga ccagcccgta tgacgggaag gtcgagttct ccggccagtc
     4801 cagctgcagc cgcatgccgc cgccgatgat gcgcaggccg tagttgcgga tccagccctg
     4861 gtcttctggc gtcgggatgc cgagggtgat gagctcgcgg accgcgcgag gggtaaggcc
     4921 gtccccgcag accttctccc gggggaaa
//
LOCUS       DATAIX010000516         1784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_117504, whole genome shotgun sequence.
ACCESSION   DATAIX010000516 DATAIX010000000
VERSION     DATAIX010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117504"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VHM65_07740"
     CDS             <1..628
                     /locus_tag="VHM65_07740"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX2705634.1"
                     /translation="FPHGRHLPPERVLAERLGVARMTLREAIAALRQSGMVQTTRGRA
                     GGTVVIYRGPDIPSAEQGPAQPSVRRGADLLDALQFRRVVEPGVAFLAASRSLSADQR
                     AWLVQAEADVRGASTPGSHRVADARLHLAIATLCGSPMLIEAVTRAQAAVHELLSAIP
                     VLPRNIDHSNADHARIVAAILAGQADVARLTMEAHCDATSALLRGLLG"
     gene            642..>1784
                     /locus_tag="VHM65_07745"
     CDS             642..>1784
                     /locus_tag="VHM65_07745"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX2705635.1"
                     /translation="MTPAAALDPETLRADIDAGRVDTVIVGFTDMQGRLQGKRIHGAF
                     FLDHVLGHGTEGCNYLLAVDVDMNTVDGYAISSWERGYGDMLFDLDLSTLYRTPGQAY
                     SATVLADLRWLDGSGPVRPSPRSVLRTQVEAAAALGLEAFAGTELEFIVFDNTYEDAW
                     DRRYRGLTPANRYNVDYSILGGSRVEPLLREIRNEMYAAGVSVESAKGECNLGQHEIA
                     FLYDRVLRTCDNHSVYKNAAKEIASAHGQALTFMAKFNEREGNSCHVHLSFRGLDGTM
                     TFADAADPLGFSPTFRSCLAGLLVTMRELTLLYAPNVNSYKRFQPGSFAPTAVAWGRD
                     NRTCSLRVVGHGAGLRVENRVPGGDVNPYLAVAAMMAGGLQLKALAD"
BASE COUNT          260 a          661 c          594 g          269 t
ORIGIN      
        1 cttcccccat ggccggcacc tcccgcccga gcgggtcctc gccgagcggc tcggggtcgc
       61 ccggatgacg ctgcgcgaag cgatcgcagc cctgcgccag tccggcatgg tgcagacgac
      121 ccgcggccgc gcgggcggca cggtggtgat ctaccgcggc ccggatatcc cgagcgccga
      181 gcagggtcct gcccagccgt cggtgcgccg cggcgccgat ctgctcgacg ccctgcagtt
      241 ccgccgggtc gtcgagccgg gcgtggcctt cctcgccgcc tcccggtcct tgtccgccga
      301 ccagcgggcc tggctcgtcc aggccgaggc ggacgtccgg ggcgcctcca cccccgggag
      361 ccatcgggtc gccgacgccc ggctccacct ggcgatcgcg accctgtgcg gcagcccgat
      421 gctcatcgag gccgtgacgc gcgcccaggc ggccgtgcac gagctgctca gcgccattcc
      481 cgtcctgcca cgcaacatcg accactccaa cgccgaccat gcacgcatcg tcgcggcgat
      541 cctggccggc caggccgacg tcgcccggct gaccatggaa gcgcactgcg acgcgacctc
      601 cgcactcctg cgcgggctcc tcggctgaga aagggctcca catgacccct gcggcagcgc
      661 tcgaccccga gaccctgcgc gcggatatcg acgccgggcg ggtggacacc gtgatcgtcg
      721 ggttcaccga catgcagggc cgtctgcagg gtaagcgcat ccacggtgcg ttcttcctgg
      781 accacgtgct cggccatggc acggaggggt gcaactacct gctcgccgtc gatgtcgaca
      841 tgaacaccgt tgatggctat gcgatctcat catgggagcg cgggtacggc gacatgttgt
      901 tcgacctcga cctgtcgacg ctgtaccgca caccgggcca ggcgtactcg gccaccgtcc
      961 tcgcggacct acgctggctc gacggcagtg gcccggtccg gccctcgccg cggtcggtcc
     1021 tgcgcaccca ggtcgaggcg gctgctgcgc tgggcctgga ggccttcgcg ggcaccgagc
     1081 tcgagttcat cgtgttcgac aacacctacg aggacgcatg ggaccggcgc taccgggggc
     1141 tgaccccggc gaatcgctac aacgtcgact actcgatcct cggcgggagc cgcgtcgagc
     1201 cgctgctgcg cgagatccgc aacgagatgt atgccgcggg ggtcagcgtg gagagcgcca
     1261 agggcgagtg caacctcggc cagcacgaga tcgcattcct ctacgaccgg gtcctgcgca
     1321 cgtgtgacaa ccacagcgtg tacaagaacg cggccaagga gatcgccagt gcgcacggcc
     1381 aggcgctcac cttcatggcc aagttcaacg agcgcgaagg caactcgtgc catgtgcacc
     1441 tgtccttccg ggggctcgac gggacgatga ccttcgccga cgccgccgac ccgctcgggt
     1501 tctccccgac gttccggtcc tgcctcgcgg ggctgctcgt cacgatgcgc gagctcaccc
     1561 tgctctatgc cccgaacgtc aactcctaca agcgtttcca gccaggctcg tttgcgccca
     1621 cggcggtggc ctggggtcgc gacaaccgga cctgctcgct tcgggtcgtc ggccatggtg
     1681 cgggactgcg ggtcgagaac cgagtgccgg gaggggacgt caacccctac ctggccgtcg
     1741 ccgccatgat ggccggcggg ctccagctca aggccctcgc ggat
//
LOCUS       DATAIX010000517         1910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_58093, whole genome shotgun sequence.
ACCESSION   DATAIX010000517 DATAIX010000000
VERSION     DATAIX010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58093"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VHM65_07750"
     CDS             <1..694
                     /locus_tag="VHM65_07750"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2705636.1"
                     /translation="HYIVHLSAGGTVPELRAARARGSRVVAETCPQYLVLDHHAYGAP
                     GGDEPRARAAVMSPPLRAPEDQDVLWAALADGTISFVGTDHCSFTLAQKAAAADFSAV
                     PNGAPGIELRLGLLYTHGVAAGRLTPERFVAVTSENAARYFGLYPRKGTLLPGSDADV
                     VVYDPEPTWVVRHEELHDAAGHTPYEGLTIAGRVRDVFLRGHHLVDDYELVPEPPPAQ
                     FLRCGLPDGGVR"
     gene            745..>1910
                     /locus_tag="VHM65_07755"
     CDS             745..>1910
                     /locus_tag="VHM65_07755"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX2705637.1"
                     /translation="MTTVTINVNGQDHDVAGEAMLVHALREQIGTLSVKVGCDDATCG
                     TCMVIADGRLVKACNRPASAFAGQRVVTTEGLSDREKAVYTHAYGETGAVQCGFCIPA
                     MVMASKALLDKSPEPTRADIVKALRTDICRCTGYRQIVDAVLLAGELLSTDAAPVRST
                     ARMRISDKAHRPDADEKVLGTGKFADDIRLPGMVFAKALRSPTPRGLLTGLNLETARA
                     HPDCLAILTAEDVPENKIGHIVNDWDVMIRVGDCTRYVGDALALVASADESRLDEILA
                     LIEVDYEPLPPVTSPQRALSPDAPLIHPRGNVLDIEHVKLGNPDHAIRDSAHVVTRKF
                     KTPWQEHAFMEPECAVAEPDGDGVRMYTSGQSVYDEQREIARMLALPRERVRSMS"
BASE COUNT          307 a          693 c          650 g          260 t
ORIGIN      
        1 gcactacatc gtccacctca gcgccggtgg gacggtgccg gagctgcgcg cggcccgggc
       61 ccgcggcagc cgggtggtcg cggagacctg cccgcagtac ctcgtcctgg accaccacgc
      121 ctacggggcc cctggcggcg acgagccgcg ggcccgcgcg gccgtgatga gcccgccgct
      181 gcgcgccccg gaggaccagg acgtgctttg ggcagcgctg gccgacggga ccatctcgtt
      241 cgtcggcacc gaccactgtt cgttcaccct cgcgcagaag gccgccgcgg ccgacttctc
      301 cgccgtcccg aacggcgccc caggcatcga gctgcgactg ggcctgctct acacccacgg
      361 tgtggcggcc ggccggctga ccccagagcg gttcgtcgcc gtcaccagcg agaacgccgc
      421 ccggtacttc gggctctacc cgcgcaaggg gaccctgctc cccggcagcg acgccgacgt
      481 cgtcgtctac gacccggagc cgacgtgggt ggtgcgccac gaggagctcc acgacgcggc
      541 cggccacacg ccatacgagg ggctgaccat cgcgggccgg gtgcgggacg tgttcctgcg
      601 cgggcaccac ctcgtcgacg actacgagct cgttcccgag cccccaccag cacagttcct
      661 gcggtgcggg cttcccgacg gaggagtcag atagtgaaca gagataccct aggcggagcc
      721 agcggccccg gtgaggatgc tgagatgact actgtcacga tcaacgtcaa cggtcaggac
      781 cacgacgtcg ccggcgaggc gatgctggtc cacgccttgc gcgagcagat cggcacgctc
      841 tcggtgaagg tgggctgcga cgacgccacg tgcggcacgt gcatggtcat cgccgacggc
      901 aggctggtca aggcgtgcaa ccgcccggca tcggccttcg ccggccagcg agtcgtgacc
      961 accgagggac tctcggaccg cgagaaggcg gtctacaccc acgcctatgg cgagaccggt
     1021 gccgtccagt gcggcttctg cattcccgcg atggtgatgg cgtccaaggc gcttctggac
     1081 aagtcgcccg aaccgacgcg cgccgacatc gtcaaggccc tgcgcaccga catctgccgg
     1141 tgcaccggtt accggcagat cgtcgacgct gtcctgctcg ccggcgagct gctcagcacc
     1201 gacgcggcgc cggtgcggtc gaccgcacgg atgcggatct ccgacaaggc gcatcgcccc
     1261 gacgcggacg agaaggtcct cggcaccggc aagttcgccg acgacatccg cctgccgggc
     1321 atggtcttcg ccaaggcgct acgctcgccc accccccggg gtctgctcac aggcctgaac
     1381 ctcgagacgg cgcgggccca cccggactgc ctggccatcc tgacggccga ggacgtgccg
     1441 gagaacaaga tcgggcacat cgtcaacgac tgggacgtca tgatccgggt cggcgactgc
     1501 acccgctatg tgggcgacgc cctggccctg gtggccagtg ccgacgagtc gaggctcgac
     1561 gagatcctcg cgctcatcga ggtcgactac gagccgttgc cgccggtcac gtctccacag
     1621 cgggccctga gcccggacgc accgctgatc cacccgcgag ggaacgtcct cgacatcgag
     1681 cacgtcaagc tgggcaaccc cgaccacgcc atccgtgact cggcgcacgt ggtgacccgc
     1741 aagttcaaga cgccgtggca ggagcacgcg ttcatggagc ccgagtgcgc ggtcgccgaa
     1801 ccggacggtg acggggtgcg catgtacacc tcgggccagt cggtgtatga cgagcagcgc
     1861 gagatcgccc ggatgctggc gctgccgaga gagcgggtcc gctccatgtc
//
LOCUS       DATAIX010000518         2628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_88263, whole genome shotgun sequence.
ACCESSION   DATAIX010000518 DATAIX010000000
VERSION     DATAIX010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88263"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..856
                     /locus_tag="VHM65_07760"
     CDS             59..856
                     /locus_tag="VHM65_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705638.1"
                     /translation="MTPAELLALFHREVRLGPVQPSPHVVVERVGPVRRAYHEDPAVP
                     RAMIEAPQGLGPDPDEVITGERDFFAARGQRVEFKVYSYDEPADLPDRLVAAGFVAED
                     VEALILGEAAAVLAGASPLPPGLRLRDVDGRADFDRIDALHEQVWGDRSEGYVDNLVR
                     ETRDHPDRIVVTVVEEVARGRVVSSARLGLEGSSFAGLWGGATLAQWRGQGLYRALVA
                     HRARIALDRGVPYLRVDASADSAPILARLGLHQVATTTPFILDPRHR"
     gene            976..2019
                     /gene="fbaA"
                     /locus_tag="VHM65_07765"
     CDS             976..2019
                     /gene="fbaA"
                     /locus_tag="VHM65_07765"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX2705639.1"
                     /translation="MPIATPEVYADMLDRAKAGSFAYPAINVSSSQTLNAALQGFADA
                     GSDGIIQVSTGGAEYFSGPSVKNMVTGSLAMAAYAEEVAKSYPVNVALHTDHCPKPKL
                     DGFVRPLIAASAERVARGGLPYFQSHMWDGSAVPLPENLQIAEELLALCKAANIILEV
                     EIGVVGGEEDGVVGEINDKLYTTPEDALATVTALGLGEQGRYMCALTFGNVHGVYKPG
                     NVKLRPEILKHAQDAIVSAQGLPAAAKPLDLVFHGGSGSLPQEIADAVNYGVIKMNVD
                     TDTQYAYTRPVAAWMLMHYDGVLKIDGEVGNKKQYDPRAWGKAGESGMAARVVEACQN
                     LRSAGTHQGPAAS"
     gene            2016..2420
                     /locus_tag="VHM65_07770"
     CDS             2016..2420
                     /locus_tag="VHM65_07770"
                     /inference="COORDINATES: protein motif:HMM:NF022793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 domain-containing protein"
                     /protein_id="HEX2705640.1"
                     /translation="MTDSLLGVPATLLPPDPAAAALAGGADATTAAASYPASSLAWAV
                     LAEASLRAGQAVTGYAYARTGYHRGLDALRRAGWRGQGPVPWEHEPNRGFLRALAALA
                     RAARDIGETDEERRCADFLRASSATAARELGF"
BASE COUNT          379 a          961 c          921 g          367 t
ORIGIN      
        1 gccaccgccg ggtcctcgtg gtaggcccgg cgcccgggcc cgccgacaag gagcctggat
       61 gacgcccgcc gagctgttgg ccctcttcca ccgcgaggtc cgcctcgggc cggtccagcc
      121 gagcccgcac gtcgtcgtcg agcgcgtcgg cccggtgcgc cgggcctacc acgaggaccc
      181 ggcggtgccg cgggcgatga tcgaggcacc ccagggcctg ggacctgacc ccgacgaggt
      241 catcaccgga gaacgggact tcttcgcggc ccgtggtcag cgggtcgagt tcaaggtcta
      301 cagctacgac gagcccgcgg acctgcctga ccgcctggtc gcggccggct tcgtcgccga
      361 ggacgtcgag gcgctcatcc ttggagaggc agccgccgtc ctggcgggtg cctcaccact
      421 accgcccggg ctgcgccttc gtgacgtcga cgggcgcgcg gacttcgacc ggatcgacgc
      481 actgcacgag caggtgtggg gtgatcgcag cgaggggtac gtcgacaacc tcgtgcgcga
      541 gacccgggac caccccgacc gcatcgtcgt caccgtggtc gaggaggtgg cccgtggccg
      601 ggtcgtgagc agtgcccggc tcggtctcga gggttcgtcg ttcgcgggcc tgtggggagg
      661 cgcgaccctc gcgcagtggc gcggtcaggg gctctaccgt gcgctcgtgg ctcaccgggc
      721 ccggatcgcc ctggaccggg gcgtccccta cctgcgggtg gacgcctcgg ccgactcggc
      781 accgatcctc gcccggctgg ggctccacca ggtcgccacg acgaccccgt tcatcctcga
      841 cccgaggcac cggtgacggc ccccgtgccc cgttgggtcc ggtgatcgcc gggtcaaagg
      901 tcaccgtcgc gtgatcgggc gaccgtggaa ggatgggggc gacgtttgaa caccgcccgc
      961 accaaggaga gactcatgcc cattgccacc cccgaggtct acgccgacat gctcgaccgg
     1021 gccaaggccg gctcgttcgc ctacccggcc atcaacgtct cgtcgtcgca gacgctcaac
     1081 gcggcgcttc agggcttcgc cgacgccggc agtgacggga tcatccaggt gtccaccggt
     1141 ggcgccgagt acttctccgg cccgtcggtc aagaacatgg tgaccggctc gctcgcgatg
     1201 gccgcctatg ccgaagaggt cgccaagagc tacccggtca acgtcgccct gcacaccgac
     1261 cactgcccga agcccaagct cgacggcttc gtccggccgc tcatcgccgc ctcggccgag
     1321 cgggtggccc gtggcgggct gccctacttc cagtcgcaca tgtgggacgg ctccgcggtg
     1381 ccgctgcccg agaacctcca gatcgccgag gagctgctcg cgctctgcaa ggccgcgaac
     1441 atcatcctcg aggtcgagat cggcgtcgtc ggcggcgagg aggacggtgt cgtcggggag
     1501 atcaacgaca agctctacac cactcccgag gacgccctgg ccacggtcac ggcactcggg
     1561 ctcggcgagc agggccgcta catgtgtgcc ctcaccttcg gcaacgtcca cggcgtctac
     1621 aagccgggca acgtcaagct gcgccccgag atcctcaagc acgcccagga cgccatcgtg
     1681 tccgcccagg gcctgccggc cgcagccaag ccgctcgacc tcgtcttcca cggcggatct
     1741 ggctcgctgc cccaggagat cgccgacgcg gtcaactacg gcgtcatcaa gatgaacgtc
     1801 gacaccgaca cccagtacgc ctacacccgc ccggtcgcgg cctggatgct gatgcactac
     1861 gacggtgtcc tcaagatcga cggcgaggtc ggcaacaaga agcagtacga cccgcgcgcc
     1921 tggggcaagg caggcgaatc cggtatggcg gcccgtgtcg tcgaggcgtg ccagaacctg
     1981 cgcagcgccg ggacacacca gggcccagcg gcctcgtgac cgacagcctg ctcggcgtcc
     2041 ccgcgacgct gctgccgccg gacccggcgg cggcagcgct ggcgggggga gccgacgcca
     2101 ccacggctgc cgcgtcatac ccggcctcgt cgttggcctg ggccgtgctc gccgaggcct
     2161 ccctgcgcgc gggtcaggcc gtgacgggct atgcctacgc ccgcaccggc taccatcggg
     2221 gcctggatgc cctgcgccgg gcgggatggc ggggacaggg gccggtgccg tgggagcacg
     2281 aacccaaccg gggcttcctt cgggccctgg cggcgctggc gcgggcggcg agggacatcg
     2341 gcgagaccga tgaggagcgt cgctgcgccg acttcctgcg ggccagctcg gccaccgcag
     2401 cacgtgagct gggcttctga ccaccgcgca ggaccgacca tcgcgcagga ccgaccaccg
     2461 cgcacgaccg accacacgga gggacagcac gactgcaggc atcggggctg tccctccgtg
     2521 tgctcggtcg tgcgcggtgg tcggtcctgc gcggtggtcg gtcctgcgcg gtggtcggtc
     2581 ctgcgcggtg gtcggtcctg cgcggtggtc agaagcccag ctcacgtg
//
LOCUS       DATAIX010000519         1667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_177525, whole genome shotgun sequence.
ACCESSION   DATAIX010000519 DATAIX010000000
VERSION     DATAIX010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177525"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VHM65_07775"
     CDS             complement(<1..671)
                     /locus_tag="VHM65_07775"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEX2705641.1"
                     /translation="MAAGTGRKLVIVESPAKAKTIASYLGGEYDVEASVGHIRDLPTP
                     SELPAEMKKGPFGKFAIDVDNGFEPYYVVDADKRKKVAELKRYLKDAGELYLATDEDR
                     EGEAIAWHLLETLKPRIPVKRMVFHEITREAIQRALHETRELDTRLVDAQETRRILDR
                     LYGYEVSPVLWRKVRQGLSAGRVQSVAMRMVVERERERMAFRTASYWDVVAELTPHRD
                     TARAA"
     gene            841..1515
                     /locus_tag="VHM65_07780"
     CDS             841..1515
                     /locus_tag="VHM65_07780"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEX2705642.1"
                     /translation="MSATRMPVLYLSHGAPPLADDPVWTRELAAWPGGIARPASILIV
                     SAHWEDAPLTVSSTTGAPLVYDFWGFPERYYTVRYDAPPAPALADEVSALLGGPAADV
                     RRDESRGLDHGAYVPLVEMYPEAGIPVLQMSMPSLDPAELFRIGAAQASNSRDHCGAH
                     PPTSPNRASERLWVVKPDPTTSVPASRSGARAAPMRNSSAGSSVGIDICRTGMPASGY
                     ISTSGT"
BASE COUNT          238 a          592 c          568 g          269 t
ORIGIN      
        1 tccgcggcgc gggccgtgtc gcggtggggg gtcagctcgg ccacgacgtc ccagtaggac
       61 gcggtccgga aggccatccg ctcgcgctcg cgctcgacga ccatccgcat cgcgacagac
      121 tggacgcgac cggcggacag cccttggcgg accttgcgcc acaggacggg gctgacctcg
      181 tagccgtaga ggcggtcgag gatgcgccgg gtctcctggg cgtcgacgag gcgggtgtcg
      241 agctcgcgcg tctcgtgcag cgcgcgctgg attgcctcac gggtgatctc gtggaacacc
      301 atgcgcttca ccgggattcg cggcttgagc gtctccagga gatgccaggc aatggcctcg
      361 ccctcgcggt cctcgtcggt ggcgagatag agctctcccg cgtccttgag gtagcgcttg
      421 agctcggcga ccttcttgcg cttgtcggca tcgacgacgt agtagggctc gaacccgttg
      481 tcgacgtcga tcgcgaactt gccgaacggg cccttcttca tctccgcagg cagctccgac
      541 ggggtcggca ggtcgcggat atggccgacc gaggcctcga cgtcatactc gcccccgaga
      601 taggaggcga tcgtcttcgc cttggccggc gactcgacga tgacgagctt gcgccctgtg
      661 cctgcggcca cgagacccct cgcattcccg acccccacgt gcagcggatt cgtcgctgct
      721 cggacgtggc gagccggagc acaccgtaag ccatgtcggt gtgcgttcgc atgccaggct
      781 gggcttacct ccgtttgcga tatatcgttg aaatttgcac tactgagtag actggctctc
      841 atgagcgcca cccggatgcc agttctctac ctcagccatg gagccccgcc gctcgcggac
      901 gaccccgtgt ggacccgcga gctggccgcg tggccgggcg ggatcgcccg tccggcgagc
      961 attctcatcg tctccgcgca ctgggaggac gccccgctca cggtctcctc gaccactggc
     1021 gctcccctcg tctacgactt ctgggggttc ccggagcgct actacaccgt ccggtatgac
     1081 gcaccgccgg ccccagcgct ggcggacgag gtctcggcgc tcctcggcgg tcccgcagcc
     1141 gacgtgcgcc gggacgagag ccgcggtctg gaccacgggg cctacgtgcc actcgtcgag
     1201 atgtaccccg aggccggcat cccagtcctg cagatgtcga tgccctccct cgacccggcc
     1261 gagctgttcc gcatcggggc ggcccaggcg tcgaactcgc gcgaccactg cggcgcccac
     1321 cccccgactt caccgaaccg ggcgagcgag aggttgtggg tggtgaagcc cgacccgacg
     1381 acgagcgtcc cggcgtcgcg cagcggcgcc agggccgccc cgatgcggaa cagctcggcc
     1441 gggtcgagcg tgggcatcga catctgcagg actgggatgc cggcctcggg gtacatctcg
     1501 accagtggca cgtaggcccc gtggtccaga ccgcggctct cgtcccggcg cacgccggcc
     1561 gcgggaccgc cgaggagcgc cgagacctcg tccgccagcg ctggggccgg cggtgcgtca
     1621 taccggacgg tgtagtagcg ctccgggaac ccccagaagt cgtagac
//
LOCUS       DATAIX010000520         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_177560, whole genome shotgun sequence.
ACCESSION   DATAIX010000520 DATAIX010000000
VERSION     DATAIX010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177560"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VHM65_07785"
     CDS             complement(<1..580)
                     /locus_tag="VHM65_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX2705643.1"
                     /translation="MPTPGSLPLLIDCDTGIDDSLALLYACASDDADIRAVTCVAGNV
                     DAAQVAINTRAVLELAGLPDVEVALGREVPLMRALVTTPETHGPRGLGDALLPPPSRS
                     LSARHAADLIITEARAHPGELTLVTLGPMTNLAVAVLREPALPRLLRRLVLMGGAYGA
                     AGNTTPVSEWNVAVDPEAMAVVLRAWSPDGPMT"
     gene            complement(580..>1517)
                     /locus_tag="VHM65_07790"
     CDS             complement(580..>1517)
                     /locus_tag="VHM65_07790"
                     /inference="COORDINATES: protein motif:HMM:NF025397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705644.1"
                     /translation="LIGNEVTDPAGIDWLGTTLDGAGPEVFCWVDSVAGVELLDARLG
                     GHERRLQVLLEVGQQGGRGGVRSVDEALTVARRVGTSGSLRLAGVTCFEGFVDGTTQP
                     ERDEAARALMDFGREVAVALEPLIAADGGTQILLSAGGSQYLDIVVEALVAPLGTRLP
                     VHGVLRSGGTVSHDHGGMDLVSPFGSVRAAGGPQLHPALEVWAPVVSMPEPGRAIAGA
                     GKRDLPHDGALPIVLAIRDTAGTLRHLDEDSGIRAVRLNDQHAWLEVGADASLAVGDL
                     VAFGIRHACTAFDRWRFIPVVDEAYDVIDAFETVF"
BASE COUNT          240 a          570 c          515 g          192 t
ORIGIN      
        1 atgtcatggg tccgtccggg gaccatgccc ggaggaccac cgccatcgct tccgggtcga
       61 cggccacgtt ccactcggac acgggcgtcg tgttgccggc cgcgccatac gccccgccca
      121 tgagcaccag tcgccgcagc aggcgcggca atgccggctc ccgcagcacc gccaccgcca
      181 ggttcgtcat cggacccagg gtcaccagcg tcagctcacc cgggtgtgcc cgggcctccg
      241 tgatgatcag gtcggccgcg tgacgagcgg acagtgaccg cgacgggggc ggcaacagcg
      301 catcacccag accacgcggg ccatgggtct cgggtgtcgt cacgagcgcc cgcatgaggg
      361 gcacctctcg gcccagcgcg acctcgacgt ccggcagacc ggccagctcc agcacggcgc
      421 gcgtgttgat ggccacctgg gccgcgtcca cgttgccggc aacgcacgtc accgcccgga
      481 tgtcggcgtc gtcagacgcg caggcgtaga gcagcgcgag gctgtcgtcg atgcccgtgt
      541 cgcagtcgat gagcagcggc agggagcccg gggtcggcat cagaacacgg tctcgaaggc
      601 atcgatgacg tcgtaggcct catccaccac ggggatgaag cgccaacggt cgaatgccgt
      661 gcaggcgtgc cggatgccga acgccaccag gtcaccgacc gcgagcgatg catcggcacc
      721 gacctccaac caggcgtgct ggtcgttcag acggaccgcc cggatgccgc tgtcctcatc
      781 gagatggcgg agcgttccgg ccgtgtcccg gatggccagc acgatgggca gcgctccgtc
      841 gtggggcagg tcgcgcttgc cggcacccgc gatggcccgc cccggctcgg gcatcgagac
      901 caccggtgcc cacacctcca gggccggatg gagctgcggc ccgcctgccg cccggacaga
      961 gccgaatggg gatacgaggt ccatgccccc atgatcgtgg gatacggtgc cgccgctgcg
     1021 cagcacaccg tgcaccggca ggcgggtgcc gagtggcgcg accagcgcct cgaccacgat
     1081 gtccaggtac tggctgccac ccgcgctgag caggatctgg gtgccgccat cggccgcgat
     1141 gagcggctcc agggcgaccg cgacctcgcg cccaaagtcc atcagcgccc gggccgcctc
     1201 gtcccgctcc ggctgcgtgg tgccgtcgac gaagccctcg aagcaggtca cgccggcaag
     1261 ccggagcgac ccactagtcc cgacccgcct ggccacggtc agcgcctcat cgaccgagcg
     1321 gacaccgccg cgcccacctt gctgcccgac ctcgagcagc acctggagtc gccgctcgtg
     1381 gccgccgagt cgcgcgtcga gcagctccac gccggccacc gaatcgaccc agcagaacac
     1441 ctccggaccc gcgccgtcga gcgtggtgcc gagccagtcg atgcccgcag ggtcggtcac
     1501 ctcgttgccg atgagga
//
LOCUS       DATAIX010000521         3056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_58409, whole genome shotgun sequence.
ACCESSION   DATAIX010000521 DATAIX010000000
VERSION     DATAIX010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58409"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VHM65_07795"
     CDS             complement(<1..304)
                     /locus_tag="VHM65_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705645.1"
                     /translation="MSASPTVAQRPAELVATTPAGAYRLLTFAVADVAEPARPGQFVA
                     LAVGGATSATLLRRSFSIHRATPAAAGHGRGTVELVVAARGPGTRWLTDAPAGTVVD"
     gene            complement(301..>3056)
                     /gene="carB"
                     /locus_tag="VHM65_07800"
     CDS             complement(301..>3056)
                     /gene="carB"
                     /locus_tag="VHM65_07800"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX2705646.1"
                     /translation="SGLHDSPTTEVLLEESIKGWKEYELEVMRDRADNVVVVCSIENL
                     DPMGVHTGDSITVAPALTLTDREYQRLRDLGIAVIREVGVDTGGCNIQFAVHPQTGRV
                     VVIEMNPRVSRSSALASKATGFPIAKIAAKMAIGYTLDEVPNDITRETPASFEPALDY
                     VVVKVPRFAFEKFPAADATLTTTMKSVGEAMSIGRCFTEALQKSLRSVERKGSSFHWS
                     APVPGPEEVDALLAESAVPTDGRIVSVQQALRAGASVEQVHEATGIDPWFLDQIELVN
                     DLAERIRTADELWPDLLRLAKRHGFSDQQLAQLRGLPEAVVRGVRHALGIRPVYKTVD
                     TCAAEFAAQTPYHYSTYDEETEVEPREREAVIILGSGPNRIGQGVEFDYSCVHASFAL
                     RDAGYDTVMVNCNPETVSTDYDTSSRLYFEPLTLEDVLEIVHAESQAGPIAGVIAQLG
                     GQTPLGLAAALKAEGVTMLGTSPEAIHLAEDRGAFGRVLAEAHLHAPRHGTAYSSPEA
                     LAVAHEIGYPVLVRPSYVLGGRGMVIVYDDASLVRYVDLATDASPEHPILVDRFLDDA
                     IEIDVDALYDGTEMYVGGIMEHIEEAGIHSGDSACTLPPVTLGAGELTRVREATLKLA
                     QGIGVVGLMNVQFALAHDVLYVIEANPRASRTVPFVAKATGVPLANAAARVMLGTSIA
                     ALRAEGVLPAHADGGMMPAHAPMSVKEAVLPFKRFRRADGAAVDSLLGPEMRSTGEVM
                     GIDPDFGCAFAKSQLAAYGGLPTHGAVFVSVANRDKRSMIFPVKRLQDLGFELYATSG
                     TADVLRRNGVHAHAVRKITERGHEGAGPGGAGLQSSIVELIRAGRVQMVVNTPSGQAA
                     RHDGYAIREATTSADLPIITTVQQLGAAVQAIEALRRGPLQVKSLQEHARDLDLYGAR
                     VAP"
BASE COUNT          424 a         1042 c         1151 g          439 t
ORIGIN      
        1 ggtcgacgac ggtgccggcc ggcgcgtcgg tgagccagcg ggtgccgggc ccgcgggcgg
       61 ccacgacgag ctcgaccgtc ccccggccat gccccgcggc ggcgggagtg gcccggtgga
      121 tcgagaacga gcgccgcagc agcgttgccg aggtggctcc tcccaccgcg agcgcgacga
      181 actggccggg gcgggccggc tcggcgacat cggcgaccgc gaaggtcagg aggcggtagg
      241 ccccggccgg cgtggtcgcg acgagctcgg ccgggcgctg ggcgaccgtg ggcgaggcgc
      301 tcacggggcg acccgggcac cgtagaggtc gaggtcccgc gcgtgctcct gcagggactt
      361 gacctgcagc gggccgcggc gcagcgcctc aatggcctgg acggccgcgc cgagctgctg
      421 gacggtcgtg atgatcggca ggtccgcgct cgtcgtcgcc tcgcggatcg cgtagccgtc
      481 gtggcgggcc gcctgcccgg acggggtgtt gacgaccatc tggacgcgcc ccgccctgat
      541 gagctcgacg atcgaggact gcagccccgc tccgccgggc ccggcgccct cgtggccccg
      601 ctcggtgatc ttgcggaccg cgtgcgcgtg aaccccgttg cggcgaagca cgtcggccgt
      661 ccccgaggtg gcgtagagct cgaaaccgag atcctgcaga cgcttgaccg ggaagatcat
      721 cgagcgcttg tcgcggttgg cgaccgacac gaagaccgca ccgtgggtcg gcaggccacc
      781 gtaggccgcg agctggctct tcgcgaaggc gcacccgaag tcggggtcga tgcccatgac
      841 ctccccggtg gagcgcatct ccgggccgag cagcgagtcc acggcggcgc cgtcggcgcg
      901 ccggaagcgc ttgaagggca gcacggcctc cttgaccgac atcggcgcgt gggccggcat
      961 catccccccg tccgcgtgcg ccgggagcac gccctcggcg cgcagcgcgg cgatcgaggt
     1021 gccgagcatg acccgggcgg cggcgttggc gagcggcacg ccggtcgcct tggcgacgaa
     1081 cggcaccgtc cgcgaggccc gggggttggc ctcgatgacg tagaggacgt cgtgggccag
     1141 ggcgaactgg acgttcatga ggcccacgac gccgatgccc tgggcgagct tgagggtcgc
     1201 ctcccggacc ctggtcagct cccccgcgcc gagggtgacc ggcgggaggg tgcacgccga
     1261 gtcgccggag tggatcccgg cctcctcgat gtgctccatg atcccgccga cgtacatctc
     1321 ggtgccgtcg tagagcgcgt cgacgtcgat ctcgatggcg tcgtcgagga accggtcgac
     1381 gaggatcggg tgctcgggcg aggcgtccgt cgcgaggtcg acgtacctca ccagggaggc
     1441 gtcgtcatac acgatgacca tcccccggcc accgaggacg taggacgggc gcacgaggac
     1501 cgggtagccg atctcgtggg cgaccgcgag agcctccggc gagctgtatg ccgtcccgtg
     1561 ccgtggcgcg tgcaggtgcg cctcggcgag gacccggccg aaggcacccc ggtcctcggc
     1621 gaggtggatc gcctcggggg aggtgccgag catcgtgacg ccctcggcct tgagcgccgc
     1681 ggcgagcccg agcggggtct ggccgccgag ctgggcgatg acccctgcga tcggtcccgc
     1741 ctgggactcg gcatggacga tctcgaggac gtcctcgagg gtgagcggct cgaagtagag
     1801 ccggctgctc gtgtcgtagt cggtcgacac cgtctcgggg ttgcagttga ccatgaccgt
     1861 gtcgtagccg gcgtcgcgca gcgcgaagct cgcgtggacg cacgagtagt cgaactcgac
     1921 gccctggccg atccggttgg gccctgagcc caggatgatg acggcctcgc gctcgcgggg
     1981 ctcgacctcg gtctcctcgt cataggtcga gtagtggtat ggcgtctggg ccgcgaactc
     2041 ggcggcgcac gtgtcgacgg tcttgtagac cgggcggatg ccgagggcat ggcggacgcc
     2101 gcggacgacg gcctcgggca gtcccctgag ctgggccagc tgctggtcgg agaagccgtg
     2161 ccgcttggcc aggcgcagca ggtccggcca cagctcgtcg gccgtgcgga tgcgctcggc
     2221 gaggtcgttg acgagctcga tctggtcgag gaaccacggg tcgatcccgg tggcctcgtg
     2281 gacctgctcg acgctcgcac cggcccgcag ggcctgctgg acactgacga tccgcccgtc
     2341 ggtcgggacg gccgactcgg cgaggagggc gtcgacctcc tcggggccgg gaaccggggc
     2401 cgaccagtgg aaggacgagc ccttgcgctc gaccgagcgc agcgacttct gcagggcctc
     2461 ggtaaagcag cgcccgatcg acatcgcctc gccgacgctc ttcatcgtcg tcgtcagggt
     2521 cgcatcggcg gccgggaact tctcgaaggc gaagcgcggg accttgacga cgacgtagtc
     2581 cagcgccggc tcgaagctcg cgggtgtctc ccgggtgatg tcgttgggca cctcgtcgag
     2641 ggtgtagccg atcgccatct tcgcggcgat cttggcgatc gggaagcccg tcgccttgga
     2701 ggccagagcg gaggaccggg acacccgcgg gttcatctcg atcacgacca cccggccggt
     2761 ctgcgggtgc acggcgaact ggatgttgca cccacccgtg tcgacgccga cctcgcggat
     2821 cacggcgatg ccgaggtcac gcagccgctg gtactcgcgg tcggtcagcg tcagcgccgg
     2881 tgcgacggtg atcgagtcac cggtgtgcac gcccatcggg tcgaggttct cgatcgagca
     2941 gacgaccacc acgttgtcgg cccggtcccg catgacctcc agctcgtact ccttccaccc
     3001 cttgatggac tcctccagga gcacctcggt ggtcggcgag tcgtgcaggc ccgaac
//
LOCUS       DATAIX010000522         4795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_88398, whole genome shotgun sequence.
ACCESSION   DATAIX010000522 DATAIX010000000
VERSION     DATAIX010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88398"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VHM65_07805"
     CDS             complement(<1..530)
                     /locus_tag="VHM65_07805"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX2705647.1"
                     /translation="MWQGRSRDEVPGPLEVVVRAVTYPAYGVVPGVCEVPKPRCPDGG
                     VLVAVAATGVCRSDWHAWLGHDPVALPHVPGHELAGTVATVGARVTRWRVGDRVTAPF
                     ACGCGQCEVCRAGDTHVCPAQTQPGFTHWGSFADLVAVHAADANVVGLPDEVGFVEAA
                     ALGCRFATAYRAVVDHG"
     gene            718..2595
                     /locus_tag="VHM65_07810"
     CDS             718..2595
                     /locus_tag="VHM65_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX2705648.1"
                     /translation="MGHYKSNLRDLEFNLFEVFGRQDILGSGPYEDVDEDTAREMLKE
                     VSRLAENELAASFVAGDRTPPVYDAATHSVRMPEAFKKSYQAYVDSGFWGVEVPGELG
                     GTVVPPSLKWAMAEMILGANPPVHLYAAAFSFAKILYLLGNEEQRTIAKWLVEKRWGA
                     TMVLTEPDAGSDVGAGRTKAVQNEDGTWNITGVKRFITSGEHDMSENILHFVLARPQG
                     AGPGTKGLSLFVIPKFHFDLATGELGERNGAYVTNVEHKMGIKVSTTCEITFGDKEPA
                     VGTLLGDVHDGIAQMFRVIENARMMVGTKAIATLSTGYLNALEYAKHRVQGADLTTPA
                     KDAPRVTITHHPDVRRSLMLQKAYAEGLRALVIYTATQQDAIDVDHMATHSEEVGRTS
                     PAAMAMRVNDLLLPLVKGVGSERAWVLLGTESLQIFGGSGFLADYPLEQYVRDAKIDT
                     LYEGTTAIQGLDFFFRKIVRDKGQALTHVAMQVQEFAKDLGQSNARLDVEREMLGRAL
                     EDVQAIVGFMVNQAMTADPRVGGEVTNLYKIGQNASRLLLATGDLIVGWLLLRQAEVA
                     EVALATRPEGVPAKDRDFYTGKIAAASFFAKNVLPKIAAERAICEATDNSLMDVPESA
                     F"
     gene            complement(2636..4045)
                     /locus_tag="VHM65_07815"
     CDS             complement(2636..4045)
                     /locus_tag="VHM65_07815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX2705649.1"
                     /translation="MARSLPRVDDVMAQTPVDRDRWADALRAGSLLVVMAGHWLMVSV
                     TPDGSITNTLSVIPALQPLTWVLQVMPLFFLVGGVAHSHSLDSLAASAHPEVAGGASY
                     GRYAAFVRARALRLLRPSGAFLAVWVALGVVAHLAGWTSGPGGRLFVAALVLVPQLLW
                     FVGIYLGVAALAPVMRRLHKRYGWSVVVVLVAGAAVADLARFAGGVESVGLANFALVW
                     LALHQVGFAWRDGAVTTRIAGLLLGAGVASLVLGVTLGPYPVSMVGLPGEPVSNMSPP
                     TLALLSQGLAIIGAAALLRGPMGALLLRPRVWRAVLMAGPFAMTAFLWHLTALMVAIV
                     AARALGVTLPSPGSATWWVTRPLWFLLLAGLTTALVRVFVRWDRGSRRAMGGDADPRR
                     WVDPVAATGAGTTVVGVLMVSIVGVDVLGNRPVFFVMGEVTPAVAFAVFLAGCLLLYV
                     ARPRLGVPVDRRGPGDDTS"
     gene            complement(4195..>4795)
                     /locus_tag="VHM65_07820"
     CDS             complement(4195..>4795)
                     /locus_tag="VHM65_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX2705650.1"
                     /translation="HAGSIVERPTSAETGPEGKSLWNTSLVFSADGRLVTTYRKIHRF
                     GFGAGEPVLMEAGERVVMVDLPDADPDDVGGSRSVRAGLSTCYDLRFPELFRLQVDRG
                     AQILLMCSCWPLARVEHWRLLGRARALENQCFLLACNTAGTHGGVTMGGRSQVVGPDG
                     VVLAEGGLDEEVLSVEVDVGAVARYRATFPVLADRRPFT"
BASE COUNT          851 a         1766 c         1612 g          566 t
ORIGIN      
        1 ccatgatcga cgaccgcccg gtatgccgtc gcgaaccggc agcccagggc tgccgcctcg
       61 acgaacccga cctcgtcggg cagcccgacc acgttggcgt ccgccgcatg gacggccacg
      121 agatcggcga acgagcccca gtgggtgaag cccggctggg tctgggccgg gcagacgtga
      181 gtgtcgcccg cccggcatac ctcgcactgc ccgcagccgc acgcgaacgg cgccgtcacc
      241 cggtcaccga cgcgccaccg cgtcaccctg gccccgaccg tcgccaccgt cccggcgagc
      301 tcgtgccccg gcacgtgcgg cagggccacg gggtcatggc cgagccacgc gtgccagtcc
      361 gaacggcaga cccccgtggc ggcgaccgcg acgagcaccc cgccgtcggg acaccggggc
      421 ttcggcacct cgcacacgcc aggcacgacg ccgtatgccg ggtaggtcac cgcgcgcacg
      481 acgacctcca ggggaccggg gacctcgtca cgggaccgac cctgccacac cgcaagagca
      541 cgcgcgggcc caggcccgcc cgctctgtgg ctcacgtctc agcccccaaa ggaccgtggc
      601 ctgttgcgca gtgcgaactc ctggcagcat cattggaagt taccggcgag taacacgtcg
      661 tcatcgggcc atgccgagcg agcgtgcgcg tcatacgggc ggcgaaagga acacaggatg
      721 ggccactaca agagcaacct gcgcgacctc gagttcaacc tcttcgaggt cttcgggcgc
      781 caggacatcc tgggcagcgg cccctacgag gacgtcgacg aggacaccgc ccgcgagatg
      841 ctcaaggagg tctcacgcct cgcggagaac gagctcgccg cgagtttcgt cgccggcgac
      901 cgcaccccgc cggtctacga cgcggcgacc cactcggtgc ggatgcccga ggcgttcaag
      961 aagtcctacc aggcctacgt cgactccggc ttctggggcg tcgaggttcc cggcgagctg
     1021 ggcggcacgg tcgtcccgcc gagcctgaag tgggcgatgg ccgagatgat cctcggcgcc
     1081 aacccgccgg tgcacctgta cgccgctgcc ttctccttcg ccaagatcct ctacctgctc
     1141 gggaacgagg agcagcgcac gatcgccaag tggctcgtcg agaagcgctg gggcgccacg
     1201 atggtgctca ccgagcccga tgccggatcc gacgtggggg ccggccggac caaggcggtc
     1261 cagaacgagg acggcacctg gaacatcacc ggggtcaagc gcttcatcac ctccggtgag
     1321 cacgacatgt ccgagaacat cctgcacttc gtcctggccc gcccccaggg cgccggtccc
     1381 gggaccaagg gcctgtcgct cttcgtcatc cccaagttcc acttcgacct cgcgaccggc
     1441 gagctcggcg agcgcaacgg cgcctacgtc accaacgtcg agcacaagat gggcatcaag
     1501 gtctcgacga cctgcgagat caccttcggt gacaaggagc ccgccgtcgg cacgctcctc
     1561 ggcgacgtcc acgacggcat cgcccagatg ttccgcgtca tcgagaacgc ccggatgatg
     1621 gtcggcacca aggcgatcgc caccctctcg accggctacc tcaacgccct cgagtacgcc
     1681 aagcaccgcg tccagggcgc cgacctcacc acgccggcca aggacgcccc gcgcgtcacg
     1741 atcacccacc acccggacgt gcgccgctcg ctcatgctcc agaaggcgta tgccgagggg
     1801 ctgcgcgcgc tcgtcatcta cacggccacc cagcaggacg ccatcgacgt ggaccacatg
     1861 gcgacccact ccgaggaggt cgggcggacc tcaccggccg ccatggcgat gcgggtcaac
     1921 gacctgctcc tcccgctcgt caagggcgtc ggctccgagc gggcgtgggt gctgctcggc
     1981 accgagtcgc tgcagatctt cggcgggtcg ggcttcctgg cggactaccc cctcgagcag
     2041 tacgtccgcg acgccaagat cgacacgctc tacgagggca cgacggcgat ccagggcctc
     2101 gacttcttct tccgcaagat cgtgcgggac aagggccagg cgctgaccca cgtggcgatg
     2161 caggtccagg agttcgccaa ggacctcggc cagagcaacg ccaggctcga cgtcgagcgc
     2221 gagatgctcg gcagggccct cgaggacgtc caggccatcg tcggcttcat ggtcaaccag
     2281 gcgatgacgg ccgacccgcg ggtaggcggc gaggtcacca acctctacaa gatcggccag
     2341 aacgcctcgc gcctgctcct cgccaccggt gacctcatcg tcggctggct gctgctgcgc
     2401 caggcggagg tcgccgaggt ggccctggcc accaggccgg agggtgtgcc ggccaaggac
     2461 cgcgacttct acaccggcaa gatcgcggcg gcctcgttct tcgccaagaa cgtcctgccg
     2521 aagatcgccg ccgagcgcgc gatctgcgaa gcgaccgaca actccctcat ggacgtcccg
     2581 gagtcggcct tctgacctac cggccggggg acaccagctg accaccgcga ggtggtcagc
     2641 tggtgtcgtc tccagggccg cgccggtcca ccgggacgcc gagccggggg cgcgcgacat
     2701 acaggaggag gcacccggcc aggaagacgg cgaaggccac cgcaggcgtc acctcaccca
     2761 tgacgaagaa caccgggcgg ttaccgagga cgtcgacccc gacgatcgag accatgagca
     2821 ccccgacgac ggtggtcccg gccccggtcg ccgcaacggg gtcgacccag cgccggggat
     2881 cggcgtcgcc acccatggcc cggcgcgagc cgcggtccca gcggacgaag acacggacga
     2941 gagccgtcgt gagcccggcc aggagaagga accacagggg gcgggtcacc caccacgtgg
     3001 cggaccctgg tgacggcaac gtcacgccga gcgcccgcgc cgccacaatg gccaccatga
     3061 gcgcggtgag gtgccagagg aacgcggtca tcgcgaacgg ccccgccatg aggacggccc
     3121 tccagacccg gggccggagc agcagagcgc ccatcggacc gcgcagcagc gccgcggcac
     3181 cgatgatggc gagcccttgc gagagcaggg ccagcgtcgg gggcgacatg ttcgacacgg
     3241 gctcgccagg cagcccgacc atactcaccg ggtagggccc cagggtcaca ccgaggacga
     3301 gggacgccac accagcaccg aggagcagcc cggcgatccg cgtcgtcacc gcaccgtcgc
     3361 gccacgcgaa cccgacctgg tgcagcgcca gccacacgag ggcgaagttg gccaggccga
     3421 ccgactccac cccgccggcg aaccgggcca ggtcggcgac cgcggcaccg gccacaagca
     3481 cgaccaccac ggaccagcca tagcgcttgt gcagccgcct catcaccgga gcgagagcgg
     3541 cgaccccgag gtagatcccg acgaaccaca acagctgcgg gacgagcacg agcgccgcga
     3601 cgaagagccg cccgccagga ccgctggtcc agccggcgag gtgcgcgacc actcccagcg
     3661 cgacccagac cgcgaggaac gcaccggacg gccggagcag ccgcagcgcg cgggcccgga
     3721 cgaacgcggc ataccggccg tatgacgctc cgccggccac ctctgggtga gccgaggcgg
     3781 ccagcgagtc gagggagtgg gagtgggcga caccaccgac gaggaagaac agcggcatga
     3841 cctgaagcac ccaggtgagc ggctgcaacg ccgggatgac cgacagggtg ttcgtgatcg
     3901 agccgtcggg agtgaccgag accatgagcc aatggccggc catgacgacg agcagcgacc
     3961 cggctcgcag tgcgtcggcc cagcggtcac ggtcgaccgg agtctgggcc atgacgtcgt
     4021 cgaccctcgg gagtgaccgt gccatggcac ccattgtcgg ctaccggggt tgtcacgcct
     4081 cacccagacg cggcttcttt cgtatggcgc ggacgtatcc gcgcccggcg gaatgatccg
     4141 cgcccgggag ggctggcggg tcttcgccgc cgtagcgcga gaccgtcacc gtggtcaggt
     4201 gaacggccgc cggtccgcga ggacggggaa cgtggcgcgg tagcgggcaa ccgcgccgac
     4261 gtcgacctcg accgagagca cctcctcgtc cagcccccct tcggcgagca cgaccccgtc
     4321 cggaccgacg acctgcgagc ggccgcccat cgtcacaccg ccgtgtgtcc cggccgtgtt
     4381 gcaggcgaga aggaaacact ggttctccag cgcccgggcg cgcccgagca gccgccagtg
     4441 ctcgactcgg gccagcggcc agcaggagca catgagcagg atctgcgcgc ctcggtcgac
     4501 ctgcagccgg aacagctcgg ggaagcgcag gtcatagcag gtcgagaggc cggcccgcac
     4561 ggaccggctg cctccgacgt catcggggtc cgcgtcggga agatccacca tgacgacccg
     4621 ttcgcccgcc tccatgagca ccggttcgcc cgcgccgaac ccgaaccgat ggatcttgcg
     4681 gtatgtcgtc acgagccttc cgtcggcgga gaagaccaag gacgtgttcc acaggctctt
     4741 gccctcgggg ccggtctcag cactggtcgg ccgctcgacg atcgagccgg cgtgc
//
LOCUS       DATAIX010000523         2594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_131488, whole genome shotgun sequence.
ACCESSION   DATAIX010000523 DATAIX010000000
VERSION     DATAIX010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131488"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VHM65_07825"
     CDS             complement(<1..384)
                     /locus_tag="VHM65_07825"
                     /inference="COORDINATES: protein motif:HMM:NF026042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705651.1"
                     /translation="MGDPQGFLRLRERELPTRRPVAVRLLDWHEVYEQQELTTLQAQA
                     GRCMDCGIPFCHNGCPLGNLIPEWNDLAWRGDWREAIERLHATNNFPEFTGRLCPAPC
                     ETACVLAIDEKVSGGSVTIKNVEVSI"
     gene            complement(377..>2594)
                     /locus_tag="VHM65_07830"
     CDS             complement(377..>2594)
                     /locus_tag="VHM65_07830"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEX2705652.1"
                     /translation="SGSKRCGSPSHRKLWVGGEYQWRREGEPHLFDPETVFRLQHATR
                     HRRYDIFKQYTARIDDQSTALMTLRGLMGFTGAEVTGREPVPIEQVEPVSEIVRRFNT
                     GAMSYGSISLEAHETLAVAMNHLGGRSNTGEGGEDEERLLDPARRSAIKQVASGRFGV
                     TSLYLTQADDLQIKMAQGAKPGEGGQLPGNKVYPWVARTRHSTPGVGLISPPPHHDIY
                     SIEDLAQLIHDLKSANPAARVHVKLVSEVGVGTVAAGVSKAHADVVLISGHDGGTGAA
                     PLTSLKHAGAPWELGLAETQQTLVVNGLRDRIVVQVDGQLKSGRDVVIAALLGAEEFG
                     FATAPLVVSGCVMMRVCHLDTCPVGIATQNPELRARFSGKPEFVETFFEYIAEEVREH
                     LAALGFRTLAEAIGHVEALDVDRAVTHWKTAGMDLTPILDAAQNVTGGTLFQSVAQDH
                     ALEKSLDHQLIALARPALDDGVQARAEIAVRNVNRTVGTMLGHEVTKAHPHGLPDHAI
                     EFTLTGSAGQSFGAFLPKGITLRLLGDANDYVGKGLSGGRIVVRPDRAAPLAAEHNVI
                     AGNVIGYGATSGEVFLRGTVGERFCVRNSGATAVVEGVGDHGCEYMTGGTVLVLGATG
                     RNFAAGMSGGVAYVLDLRPDKVNPELVDLLPLRGADAMTVRALLETHRGWTESAVAER
                     LLAEWDADWQGVSARFTLVLPRDYQRVLEVRATAEAEGLDPDSPEVWTRIMEASHG"
BASE COUNT          381 a          909 c          904 g          400 t
ORIGIN      
        1 gatcgacacc tcgacgttct tgatggtgac cgagccgccg gagaccttct cgtcgatggc
       61 gagcacgcag gcggtctcac acggcgccgg gcacagccgg ccggtgaact ccgggaagtt
      121 gttcgtggcg tggaggcgct cgatcgcctc ccgccagtca ccccgccagg ccaggtcgtt
      181 ccactcggga atgaggttgc cgagcggaca tccgttgtgg cagaagggga ttccgcagtc
      241 catgcagcgc ccggcctggg cctgcagcgt cgtcagctcc tgctgctcat agacctcgtg
      301 ccagtccagg agccgcactg ctacggggcg acgggtcggc agctcgcgct cacgcaggcg
      361 caggaatcct tgcgggtcac ccatgagagg cctccatgat ccgggtccag acctcggggc
      421 tgtcgggatc gagcccctcg gcctcggccg tcgcccgcac ctcgaggacg cgctggtagt
      481 cgcggggcag gacgagggtg aaccgcgccg agacgccctg ccagtcggca tcccactcgg
      541 cgagcagccg ctccgcgacg gccgactcgg tccagccgcg atgtgtctcg agcagggcgc
      601 gcacagtcat ggcgtcggcc ccgcgcaacg gcaagaggtc gacgagctcg gggttgacct
      661 tgtccgggcg caggtcgagc acatacgcca cgccaccgga catcccggcc gcgaagttgc
      721 gccccgtcgc cccgagcacg aggacggtgc cgccggtcat gtactcgcag ccatggtcac
      781 ctactccctc gaccaccgcc gtggcaccgg agttgcgcac gcagaaccgc tcccccaccg
      841 tgccgcgcag gaacacctcg ccactcgtgg cgccataacc gatgacgttg ccggcgatga
      901 cgttgtgctc ggcggccaac ggtgccgcgc gatcggggcg gacgacgatc cggccacccg
      961 aaaggccttt gccgacgtag tcgttcgcgt cgccgagcag ccgcagggtg atccccttgg
     1021 gaaggaacgc tccgaaagac tggcccgcgg acccggtgag ggtgaactcg atcgcgtggt
     1081 cgggcagccc gtgcggatgg gccttggtca cctcgtggcc gagcatggtg ccgaccgtgc
     1141 ggttgacgtt acgcaccgcg atctcggccc gggcctgcac accgtcgtcg agtgcggggc
     1201 gcgcgagcgc gatgagctgg tggtcgaggg acttctccag cgcgtgatcc tgagccaccg
     1261 actggaacag cgtcccgccc gtgacgttct gggcggcgtc gaggatcggc gtgaggtcca
     1321 tccccgccgt cttccagtgc gtcaccgcgc ggtcgacatc gagggcctcg acatggccga
     1381 tcgcctcggc gagggtccgg aagccgagcg ccgcgaggtg ctcgcggacc tcctcggcga
     1441 tgtactcgaa gaacgtctcc acgaactcgg gcttgcccga gaacctcgcc cgcagctccg
     1501 ggttctgcgt cgcgatgccg accgggcagg tgtcgagatg gcagacccgc atcatgacgc
     1561 agccactgac gacgagcggg gcggtcgcga agccgaactc ctccgcgccg agcaatgccg
     1621 cgatcacgac gtcgcgcccg ctcttgagct ggccgtcgac ctggacgacg atgcggtcgc
     1681 gcaggccgtt gacgacgagc gtctgctggg tctcggcgag gcccagctcc cacggcgcgc
     1741 cggcgtgctt gagcgaggtg agcggcgcgg cgccagtgcc tccgtcgtgt cccgagatga
     1801 gcacgacgtc ggcgtgggcc ttggagaccc ccgccgcgac cgtcccgacg ccgacctcgg
     1861 agacgagctt gacgtgcact cgcgccgcgg ggttggcgct cttgaggtcg tgaatcagct
     1921 gggcaaggtc ctcgatcgag tagatgtcat ggtgcggcgg cggcgagatg agcccgaccc
     1981 cgggggtcga gtgccgggtc cgggccaccc atgggtagac cttgttcccc gggagctgcc
     2041 caccctcacc gggcttggcg ccctgggcca tcttgatctg caagtcgtcg gcctgggtca
     2101 ggtagaggct cgtcacaccg aagcggcccg aggcgacctg cttgatcgcc gagcgtcgcg
     2161 ccgggtcgag cagccgctcc tcgtcctcgc cgccctcccc cgtgttggag cggccaccga
     2221 ggtggttcat cgcgaccgcc agcgtctcgt gcgcctccag gctgatcgag ccatagctca
     2281 tggccccggt gttgaaccgc cggacgatct cactgaccgg ctcgacctgc tcgatcggga
     2341 ccggctcgcg accggtgacc tcggcaccgg tgaagcccat cagcccccgc agggtcatca
     2401 gcgccgtcga ctggtcgtcg atgcgcgcgg tgtactgctt gaagatgtcg tagcggcggt
     2461 ggcgggttgc gtgctggagc cggaacacgg tctccgggtc gaagaggtgc ggctcgccct
     2521 cgcgccgcca ttggtactcc ccgcccaccc acagcttgcg gtgcgagggt gagccgcacc
     2581 tcttcgaccc ggag
//
LOCUS       DATAIX010000524         2275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_88513, whole genome shotgun sequence.
ACCESSION   DATAIX010000524 DATAIX010000000
VERSION     DATAIX010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88513"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VHM65_07835"
     CDS             complement(<1..396)
                     /locus_tag="VHM65_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705653.1"
                     /translation="MPSTELAGYANLALYSAMVVFAVTMLVFTAHLAALGPPADLADA
                     EESAGDKILVGAGAASADDSGPAGPAEPGPSAATIPDSGIGGAAPADSMSSRARSLGS
                     VGISLTWLATLLLVAAVAMRGIAVTRPPWG"
     gene            complement(495..2159)
                     /locus_tag="VHM65_07840"
     CDS             complement(495..2159)
                     /locus_tag="VHM65_07840"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEX2705654.1"
                     /translation="MSTGTATVPAVTQPPLGPAGWARWAWRQLTSMRTALFLLMLLAV
                     AAVPGSVFPQRSIDAGRVADYLNDNPTLGGWLDRLSFFDVYSSPWFSAIYLLLVVSLL
                     GCIVPRSRSHWQTLRSVPPRAPARLERLAEHLELTYDGPPAEALEVTRRALLRRRMRV
                     YSHDGGSLSAEGGLLRETGNLVFHIAICVIVVGVAIGYLFGWRGDVIVPVGERFSSTV
                     TSYDTFSPGPLVDAETLPPFSLKVDAMDVRFEENVGGRQLGAPRRFAATVTTRETPAA
                     PALSQEISVNGPLELGGAGVYLLGNGYAPMITVRDASGAVLYRQATPFLPRDNNYASV
                     GAVKVIAASPKELGFSGVFLPTADWSSQEGPRSLFPDLGDPELALSVWEGTLAPGGRP
                     QSVYTLTTTGMTQVTSAEGTALFIRLKPGQSFELPGGRGSITFESVDRWAGLSVRVDP
                     GKGLTLVAALAALFGLMATLLIKRRRVFVRVAPESDGGASTSAGPARAGRPGGPDGAR
                     GAAPRTVVTIGAMAKSADPALARFLASVAEDIPAVADPAVQQRAET"
BASE COUNT          349 a          830 c          810 g          286 t
ORIGIN      
        1 gccccagggc gggcgtgtga cggcgatccc ccgcatcgcg acggccgcca cgagcaacag
       61 ggtcgcgagc caggtcaggc tgatccccac cgagccgagc gaccgcgccc gggacgacat
      121 ggagtcggcc ggagccgccc ccccgattcc cgagtccggg atcgtcgcgg ccgacgggcc
      181 gggctcagcc gggcccgccg ggccggagtc gtcggccgac gcggcgcccg cacccacgag
      241 gatcttgtcg cccgcgctct cctcggcatc ggccaggtcg gccggtgggc cgagcgccgc
      301 caggtgcgcg gtgaagacga gcatggtgac cgcgaagacc accatggccg agtagagcgc
      361 gaggttggcg tagcccgcca gttcggtcga aggcacctgt ccgtccgttc ctactcgttg
      421 tcccgcactg ctggcttcgt cactgctcgt tgctgcactg ctcgttgctg cactgctcgt
      481 tgctgcactg ctcgttatgt ctctgctcgt tgctgcactg ctgggtcagc cacggccggt
      541 atgtcctcgg ccaccgaggc gaggaaccgc gccagcgccg ggtcggcgga cttggccatc
      601 gcgccgattg tcaccaccgt acgcggtgcg gccccccgcg ctccatccgg cccgccggga
      661 cgacccgccc gcgcgggacc tgcggacgtc gatgcgccgc cgtcgctctc cggggccacc
      721 cgcacgaaga cgcgccggcg cttgatgagc agggtcgcca tgagaccgaa gagagccgcc
      781 agggccgcga cgagcgtcag ccccttgccg gggtcgaccc gcaccgagag cccggcccac
      841 cggtcgaccg actcgaaggt gatgctcccg cgcccgccgg gcagctcgaa gctctgaccc
      901 ggcttgagcc ggatgaacag cgccgtgccc tccgcagagg tgacctgggt catccccgtc
      961 gtcgtcagcg tgtagaccga ctgcgggcgg ccccccgggg cgagcgtgcc ctcccagacc
     1021 gacagcgcca gctcggggtc gccgaggtcg gggaagagcg agcgtggccc ctcctgcgac
     1081 gaccagtccg cggtcggcag gaacaccccg gagaagccga gctccttcgg gctggccgcg
     1141 atcaccttga ccgccccgac cgaggcgtag ttgttgtccc gcggcaggaa cggggtcgcc
     1201 tggcggtaga gcaccgcgcc actcgcgtcg cgcaccgtga tcatcggggc atagccgttg
     1261 ccgagcaggt agaccccggc cccgccgagc tccaacggcc cgttgacgct gatctcctgg
     1321 gagagggccg gcgcggccgg ggtctcccgc gtggtcacgg tggcggcaaa ccgccgcggt
     1381 gccccgagct ggcgtccccc gacgttctcc tcgaagcgca cgtccatcgc gtcgaccttg
     1441 agcgagaacg gcggcagcgt ctcggcatcg acgagcggcc ccgggctgaa ggtgtcgtat
     1501 gacgtgaccg tcgaggagaa ccgctccccg accggcacga tgacatcgcc gcgccagccg
     1561 aagaggtagc cgatcgccac cccgacgacg atgacacaga tcgcgatatg gaagacgagg
     1621 ttgcccgtct cgcgcagcag accaccctcg gcggacagcg acccgccgtc gtggctgtac
     1681 acccgcattc gccgtcgcag caacgccctc cgggtgacct cgagcgcctc ggcgggcggg
     1741 ccgtcatacg tcagctcgag gtgttcggca agccgctcga gccgcgcggg cgcacggggc
     1801 gggaccgagc gcagcgtctg ccagtggctg cggctgcgcg ggacgatgca gccgagcagc
     1861 gacacgacga gcagcaggta gatcgccgag aaccacggcg aggagtagac gtcgaagaac
     1921 gagaggcggt cgagccagcc ccccagcgtc gggttgtcgt tgaggtagtc ggcgacccgc
     1981 ccggcgtcga tcgagcgctg cgggaagacc gacccgggga cggcggcaac cgcgagcagc
     2041 atgagcagga acagcgcggt ccgcatgctc gtgagctggc gccaggccca gcgagcccat
     2101 ccggccgggc cgagcggtgg ctgggtgacc gcgggaacgg tcgcggtgcc ggtgctcacc
     2161 tagatcaccg ccacgaagcc gccgaagcgg gcctggatct cggccacgag gccctcccag
     2221 agtccggtga cgagcaggac ccccacgacg agaagcatgg cgccgcccgc gagct
//
LOCUS       DATAIX010000525         2615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_177783, whole genome shotgun sequence.
ACCESSION   DATAIX010000525 DATAIX010000000
VERSION     DATAIX010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177783"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VHM65_07845"
     CDS             <1..796
                     /locus_tag="VHM65_07845"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX2705655.1"
                     /translation="AIAGASYQGVFRNPLADPYLLGAAAGAGLGATLVIAYAPTMGLG
                     PVSVLPLAAFAGALGGVGLAYGLGAAAARGGGGNATLLLAGIAVAAFLTAMQTLVQQS
                     HVEDLRVIYSWILGQLGTADWPTLRLMLPYAVLAVVVLLLAARALDVLAVGEDEAASL
                     GIRPRRVRFVVLAAASLGTAAAVAVSGLIGFVGLVVPHIVRRLAGGSYRVVLPVSLGV
                     GAAFLVSADLLARTLIAPAELPIGVVTAFVGAPFFAVLLRVGGNST"
     gene            793..1692
                     /locus_tag="VHM65_07850"
     CDS             793..1692
                     /locus_tag="VHM65_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2705656.1"
                     /translation="MSAQPVWRAGPALPAPGLPTPTPTPAPALQTCGVRVRAGRAVLL
                     DRVDITVPVGSWTCIVGPNGAGKSTLLRAFAALVKHEGEIDVHGRPLAAMTPRERARC
                     LGYAPQNPVIPAITVGEYVMLGRTPYRSLLAPARDADVHVVEQSLRDLDLAHLAPRQL
                     ASLSGGEKQRAVLARAIAQQSRILLLDEPTAALDLGHAQAVLELVDRLRREHGLTVLS
                     TLHDLALAGQYADALVLLSGGRVVVSGPAQVVLTPGHLAEHYGALAQVSSGPDGVRVH
                     PVRPSAPARGQARAEPAPLGDES"
     gene            1689..2435
                     /locus_tag="VHM65_07855"
     CDS             1689..2435
                     /locus_tag="VHM65_07855"
                     /inference="COORDINATES: protein motif:HMM:NF014060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HEX2705657.1"
                     /translation="MTDPVHDVELGPAGPAVEPGPVVLGTRAESGTPWPVLSWSPPQQ
                     WRAIASTVVGGGIGPLAWWVNAMVAKDYHRSDPDAHVHEIAAALGLPVDRPGVGMLTA
                     ADVRAATTARDGGVAVCATVGLGWPVWAAAQEDTIAREHDVREPPPPGTINILVTVPV
                     PLSGAALVNAVATATEAKTQALLETGFQASGTSSDAICIACPVPDPARTLELYGGPRS
                     LWGARVARAVHAAVCSGARAWPPALGIPGQ"
BASE COUNT          281 a         1021 c          941 g          372 t
ORIGIN      
        1 cgcgatcgcg ggcgcgtcat accagggggt gttccgcaac ccgctcgccg acccctacct
       61 gctcggggcg gccgcggggg cggggctcgg cgcgaccctg gtcatcgcct acgccccgac
      121 gatgggtctc ggcccggtgt ccgtcctgcc gctcgccgcc tttgccgggg cgctcggtgg
      181 cgtcgggctc gcctacggcc tcggtgcggc cgcggcccgg ggtggcgggg gcaacgcgac
      241 gctgctgctc gccgggatcg cggtcgccgc cttcctcacg gccatgcaga ccctcgtgca
      301 acagagccac gtcgaggacc tgcgggtcat ctactcgtgg atcctcggcc agctcggcac
      361 cgccgactgg ccgacgctgc gcctcatgct cccgtatgcc gtgctcgcgg tcgtcgtgct
      421 gctgctcgcg gcgcgggcgc tcgacgtgct cgccgtcggc gaggacgagg ccgcctcact
      481 ggggatcagg ccccggcggg tgcgcttcgt cgtgctggcg gcggcctccc tcgggacggc
      541 tgccgcggtc gcggtgagcg ggctcatcgg cttcgtcggg ctcgtcgtcc cgcacatcgt
      601 gcggcgcctg gccggcgggt cctaccgcgt ggtgctcccg gtgtccctcg gtgtgggggc
      661 ggcgttcctc gtcagcgccg acctgctcgc ccggaccctc atcgccccgg ccgagctgcc
      721 gatcggagtc gtgacggcgt tcgtcggggc gccgttcttc gccgtgctgc tgcgggtcgg
      781 ggggaacagc acgtgagcgc ccagccggta tggcgcgccg gacccgcgtt gccggctcct
      841 ggtctgccga cgccgacgcc gacgccggcg ccggcgctgc agacgtgtgg cgtccgggtc
      901 cgggccggcc gggccgtcct gctcgaccgg gtcgacatca cggtgcccgt cggctcgtgg
      961 acctgcatcg tcggacccaa cggcgcgggg aagtcgaccc tgctgcgcgc gttcgcggcc
     1021 ctggtcaagc acgagggtga gatcgacgtg cacggtcgtc cgctcgccgc gatgaccccg
     1081 cgcgagcggg cccggtgcct gggctacgcc ccgcagaacc cggtgatccc ggccatcacg
     1141 gtgggcgagt acgtcatgct cggccgcacc ccctaccgtt ccctcctggc gccggcccgc
     1201 gacgccgacg tgcacgtcgt cgagcagtcg ctgcgggatc tcgacctcgc ccacctcgca
     1261 ccgcgacagc tcgcctccct ctccggcggg gagaagcagc gggccgtgct cgcccgggcg
     1321 atcgcccagc agtcccggat cctcctgctc gacgagccga ccgcggcact cgacctcggc
     1381 cacgcccagg cggtcctcga gctcgtcgac cggctgcgcc gcgaacacgg gctcaccgtc
     1441 ctcagcacgc tgcacgacct cgcgctcgcc gggcagtatg ccgacgcact cgtcctgctc
     1501 tccggtggcc gggtcgtcgt gtccgggccc gcgcaggtcg tcctgacccc cggccatctc
     1561 gccgagcact acggcgccct ggcccaggtg tcgagcgggc cggacggcgt gcgcgtgcac
     1621 ccggtgcgcc cgtcggctcc ggcacgcggg caggcgcgcg ccgagccggc cccgctgggc
     1681 gacgagtcgt gaccgacccg gtccacgatg tcgaacttgg gccggccggg ccggccgtcg
     1741 agcccgggcc ggtggttctc gggacgcgcg ccgagagcgg aacgccctgg ccagtcctgt
     1801 cgtggtcgcc tccgcagcag tggcgggcca tcgcgagcac cgtcgtcggc ggcgggatcg
     1861 ggccgctcgc gtggtgggtc aacgcgatgg tcgccaagga ctaccaccgc agcgacccgg
     1921 acgcccacgt ccacgagatc gccgcagccc tcggcctgcc ggtggaccgg cccggcgtcg
     1981 ggatgctcac cgccgccgac gtccgtgccg ccaccacggc ccgggatggc ggtgtcgccg
     2041 tctgcgccac ggtcggactc ggatggcccg tctgggccgc cgcgcaggag gacacgatcg
     2101 cccgcgagca cgacgtccgc gagcccccgc cgccgggcac catcaacatc ctcgtcaccg
     2161 tgcccgtccc gctgtcgggc gcagccctcg tcaacgcggt cgccaccgcg accgaggcca
     2221 agacccaggc gctgctggag acggggttcc aggcatccgg cacctcctcc gatgccatct
     2281 gcatcgcctg cccggtcccg gacccagcca gaacccttga gctgtatggc ggtccgcgct
     2341 ccctctgggg ggcgcgcgtc gcccgtgcgg tgcacgctgc cgtctgctca ggtgcgcgcg
     2401 cctggccccc ggcgctcggc atacccgggc agtgacgagg cgccgagctg gcgagcgcca
     2461 taccgtgacc cccccgctcg tcaggtcgcg aggtaggccc gaagtgcctt gatcgacacc
     2521 atgacctgga gcccctcggc cgggcaccgt tcgaagccgg cgttggcgag gtagaaggcc
     2581 ttggcgtgct cgtcccggca gtggacgagc accgc
//
LOCUS       DATAIX010000526         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_177828, whole genome shotgun sequence.
ACCESSION   DATAIX010000526 DATAIX010000000
VERSION     DATAIX010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177828"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1592
                     /locus_tag="VHM65_07860"
     CDS             <1..>1592
                     /locus_tag="VHM65_07860"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2705658.1"
                     /translation="VVTMAGEPADYRFADDAPVIAVSAHEAAEPGANDWFDLGVTVSV
                     AGEDVPFQTLFTALASDEDHLILDSGTWFRLDREEFATLRRLIVEARSLQDRPGQTLR
                     ITPYQAGLWEELVELGVVASQSERWERTVTGLLSPEEMPTPDLPAGLTATLRPYQVDG
                     YRWLTQIWDLGLGGILADDMGLGKTLQMLALAQRAMEAGELTEPVLIVAPTSVLGTWA
                     SEAARFTPDLPVVVLAETMRRRGGELRDAIAGAALVVTSYAIFRIDEEHFQGQRWRAL
                     VLDEAQFVKNHQAKTYQCARRLEAPVKFAITGTPLENSLMDLWALLSIVAPGVFPSPQ
                     RFGEIYRRPIEAGGAPEVLASLRRRIRPLMLRRTKELVASELPPKQEQVVQIALAPGH
                     RRTYDRHLQRERQRVLGLIEDDFNANRIAILKSLTTLRLMALHPTLADPAYSATVASA
                     KVEALIEQLREVASEGHRALVFSQFTRFLAMIRERLDAEGITYSYLDGRTRDRTARIE
                     GFRSGTDPVFLISLKAGGFGLT"
BASE COUNT          226 a          574 c          553 g          239 t
ORIGIN      
        1 cgtcgtgacg atggcgggcg agccggccga ctatcggttc gccgacgacg cccccgtcat
       61 cgcggtgtcg gcgcacgagg cggccgagcc gggcgccaac gactggttcg acctcggcgt
      121 gaccgtctcg gtggcgggtg aggacgtgcc cttccagacc ctcttcacgg ccctcgccag
      181 cgacgaggac cacctcatcc tcgacagtgg cacgtggttt cgcctggatc gggaagagtt
      241 cgccaccctg cgccgcctca tcgtcgaggc ccgctcgctc caggaccggc ccgggcagac
      301 gctgcggatc acgccatacc aggccggtct gtgggaggag ctcgtcgagc tcggtgtcgt
      361 cgcctcccag agcgagcgtt gggagcgcac ggtcaccggc ctgctcagcc ccgaggagat
      421 gcccaccccc gacctcccgg ccggtttgac ggcgaccctg cgtccctatc aggtcgacgg
      481 ctaccgctgg ctcacccaga tctgggacct gggcctcggc ggcatcctgg ccgacgacat
      541 gggcctgggc aagacgctgc agatgctcgc cctggcccag cgggcgatgg aggccggcga
      601 gctgaccgag cccgtgctca tcgtcgcgcc gaccagcgtg ctgggcacct gggcgtccga
      661 ggcggcgcgg ttcaccccgg acctgcccgt ggtcgtgctc gccgagacga tgcggcgtcg
      721 cggtggcgag ctgcgcgatg ccatcgcggg ggcggcgctg gtcgtgacgt catacgcgat
      781 cttccggatc gacgaggagc acttccaggg ccagcggtgg cgcgcgctcg tcctcgacga
      841 ggcgcagttc gtcaagaacc accaggccaa gacctaccag tgtgcccggc ggctcgaggc
      901 gccggtgaag ttcgcgatca ccgggactcc gctggagaac tcgctcatgg acctgtgggc
      961 gctgctgtcg atcgtcgcgc ccggggtgtt cccgagtccg cagcggttcg gggagatcta
     1021 ccggcgcccg atcgaagccg gcggggctcc cgaggtgctc gcgtcgctgc gccggcgaat
     1081 ccgcccgctc atgctgcgcc ggaccaagga gctcgtcgcg tccgagctgc cgcccaagca
     1141 ggagcaggtg gtgcagattg ccctggcgcc cgggcatcgg cgcacctatg accggcatct
     1201 gcagcgcgag cgacagcgcg tcctggggct catcgaggac gacttcaacg ccaaccgcat
     1261 cgcgatcctc aagtccctca cgacgttgcg gctcatggcc ctgcacccca cgctggccga
     1321 cccggcctac tcggccaccg tggccagcgc caaggtcgag gcgctgatag agcagctgcg
     1381 ggaggtggcg tcggaggggc atcgggcgct cgtcttcagc cagttcaccc gcttcctcgc
     1441 gatgatccgc gaacggctcg acgccgaggg gatcacctac tcctatctcg acggccggac
     1501 ccgggaccgg accgcgcgga tcgagggctt ccggtccggc accgacccgg tgttcctcat
     1561 cagcctcaag gccggcgggt tcgggctcac cc
//
LOCUS       DATAIX010000527         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_221107, whole genome shotgun sequence.
ACCESSION   DATAIX010000527 DATAIX010000000
VERSION     DATAIX010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221107"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..978)
                     /locus_tag="VHM65_07865"
     CDS             complement(34..978)
                     /locus_tag="VHM65_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2705659.1"
                     /translation="MSELATDRTYAQVYAASIDDPETFWGDAAKDIDWITPPTRVLDA
                     SRPPFYRWFPGATLNTCFNALDRHVEAGRGGQVALCYDSPVTGTKRAYTYAEVLAEVR
                     RLAGALAGAGVGKGDRVVVYMPMVPEAAFAMLACARIGAIHSVVFGGFAPAELAARID
                     DAQPKLILSASCGIEPSRVVPYKPFLDEGIARSEHKPDLVVILQREQGLAPIGERDID
                     WQEFVGSAAGDAGADCVEVEATDPLYILYTSGTTGKPKGIYRDNGGHAVALRWSMRNI
                     YNVEPGETMFTASDVGWVVGHSYIVYAPLLTGAPPGVL"
     gene            1274..1408
                     /locus_tag="VHM65_07870"
     CDS             1274..1408
                     /locus_tag="VHM65_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705660.1"
                     /translation="MVRIFSNIISVQSPLVATPVAERHRRIEAASALRIQQRVLGGRL
                     "
     gene            complement(1516..>2163)
                     /locus_tag="VHM65_07875"
     CDS             complement(1516..>2163)
                     /locus_tag="VHM65_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2705661.1"
                     /translation="SMDGSFAPLDRICDLADSYGALVFVDDSHAVGFVGEGGRGTPEL
                     FGVRDRVDIITGTLGKALGGASGGYVSAHEEIVDLLRQRARPYLFSNSVAPAVVAGSL
                     AALDLAERSGAARETLTRHTALFRELMTEAGFDLLPGRHPITPVMFPGRDGARRATAI
                     ADHMLSHGVYVIAFSYPVVPVGKARIRVQLSAAHSEDDVRACVDAFIAAWAAVGD"
BASE COUNT          350 a          757 c          738 g          318 t
ORIGIN      
        1 gtgcgcaggt ggacccagtg ctcggccgga tagtcataga acgccaggag gcgccccggt
       61 gagcaggggt gcatagacga tgtaggagtg cccgacgacc cacccgacgt cgctggctgt
      121 gaacatcgtc tcgcccggct cgacgttgta gatgttgcgc atcgaccagc gcagcgcgac
      181 cgcgtggccg ccgttgtcac ggtagatgcc cttgggcttc ccggtcgtgc cggacgtgta
      241 gaggatgtag agcgggtcgg tggcctcgac ctcgacgcag tcggcgccgg catcgcccgc
      301 ggcactcccc acgaactcct gccagtcgat gtcccgctcc ccgatcgggg caaggccctg
      361 ctcgcgctgc aagatcacga cgaggtcggg cttgtgctcc gaccgggcga tcccctcgtc
      421 gaggaacggc ttgtacggca ccacccggct gggctcgatg ccgcacgacg ccgacaggat
      481 gagcttgggc tgcgcgtcgt cgatccgagc ggcgagctcg gccggggcga acccaccgaa
      541 gacgaccgag tggatcgccc cgatccgcgc gcaggcgagc atggcaaaag ccgcctcggg
      601 gaccatgggc atgtagacga cgacgcggtc gcccttgccc actccggccc cggccagcgc
      661 tccggcgagg cgtcgcacct cggccagcac ctcggcatac gtgtaggccc gcttcgtgcc
      721 ggtcacggga gagtcgtagc acagggcgac ctgtcccccg cgcccggcct cgacatggcg
      781 gtcgagcgcg ttgaagcagg tgttgagcgt cgcacccggg aaccaccggt agaacggtgg
      841 cctggaggcg tcgagcaccc tcgtcggcgg ggtgatccag tcgatgtcct tggccgcgtc
      901 accccagaag gtctctgggt catcgatgct tgcggcgtag acctgcgcgt acgtccggtc
      961 ggtggctagc tcgctcatgg gcagaactgt gccgcgggcc agccccgctc gccagttgga
     1021 cgggccgagg tgcgccctgg acgcatggtg gtgcgccgaa cggtcctcca ccaccacggc
     1081 gcatggcggg aatgccccgc cccggtgggc ggttatccac agggcgcccg atccgcccat
     1141 agacgatgtg gccccagcag tcgggccaaa ggcgctttag aggtttcctc taatgttgtt
     1201 actcacgaga cagattttca gtacgtttga cacggcatcc atagcacatc agagaccatg
     1261 atacaataga tgcatggtac gaatcttcag caatatcatc tccgtccagt cccccctcgt
     1321 ggcgaccccg gtcgccgaga ggcaccgccg catcgaggcg gcctcggccc tgcgcatcca
     1381 gcagcgtgtc ctgggcggtc gcctgtaagc acgcggcgtc gcccaggagc ccaccgacga
     1441 tcgggccccc cctccgctct cgatgcggac gggggctttc tgtctgccgg gcggggcagc
     1501 ctcggtcgag ccggctcagt ccccgacggc cgcccacgca gcgatgaacg cgtcgacgca
     1561 ggcccgcacg tcgtcctcgg agtgggccgc gctgagctgg acccggatcc gggccttgcc
     1621 caccggcacc acagggtagg agaacgcgat gacgtacacc ccgtggctga gcatgtggtc
     1681 ggcgatcgcc gtcgcgcgcc gggccccgtc ccggccgggg aacatcaccg gcgtgatcgg
     1741 atgcctgccc ggcagcaggt cgaagcccgc ctcggtcatc agctcgcgaa aaagggctgt
     1801 atggcgcgtc agagtctcgc gcgccgcccc cgagcgctca gccaggtcga gcgcggccaa
     1861 ggacccggcc acgaccgcgg gagccaccga gttggagaag aggtagggcc gcgcccgctg
     1921 ccggagcagg tcgacaatct cctcatgggc actgacgtag ccgccggacg ccccgcccag
     1981 ggccttgccg agcgtcccgg tgatgatgtc gacccggtcg cggacaccga acagctccgg
     2041 tgtccctcga ccgccctccc cgacgaagcc gaccgcgtgc gagtcgtcga cgaacaccaa
     2101 ggcgccgtag gagtcggcga ggtcgcagat ccggtcgagc ggggcgaacg agccgtccat
     2161 cga
//
LOCUS       DATAIX010000528         2577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_28309, whole genome shotgun sequence.
ACCESSION   DATAIX010000528 DATAIX010000000
VERSION     DATAIX010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28309"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..455)
                     /locus_tag="VHM65_07880"
     CDS             complement(192..455)
                     /locus_tag="VHM65_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005326607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX2705662.1"
                     /translation="MLELRVVTSEGEPVEALSWQERSLCAQTDPEAFFPEKGGSTREA
                     KKVCLGCDVRAECLEYALANDERFGIWGGLSERERRKLKKRAV"
     gene            692..1741
                     /gene="cofD"
                     /locus_tag="VHM65_07885"
     CDS             692..1741
                     /gene="cofD"
                     /locus_tag="VHM65_07885"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX2705663.1"
                     /translation="MSVPDPSPGLRITALAGGVGGARFLRGLLAYLAETTDLAGSTVT
                     VIGNTGDDITLFGLRVCPDLDTLVYTLGGGVHEGQGWGRADETYAVQGELSAYGVGPQ
                     WFGLGDRDFGTHVARSLWLGQGLTLSEVTARLATRWGLPERGVTLLPMTDMPVETHVV
                     VEDEQGRRAVHFQEWWVRMRAAVPAQRFVVAGMDRADAAPGVLDAIRQADLVILPPSN
                     PVVSIGIILGVPGVRDALRGTRAPVVGVSPLIDGRPVRGHADACLRAIGIESTSAAVA
                     GLYADFLDGWLVDSLDTAGSIDQTDPTHARRAARGARPAVRPRPLLMVDLTSAAAIAG
                     AAVELGRELGARPAA"
     gene            1744..>2577
                     /gene="cofE"
                     /locus_tag="VHM65_07890"
     CDS             1744..>2577
                     /gene="cofE"
                     /locus_tag="VHM65_07890"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX2705664.1"
                     /translation="MPSDPAPLEILPLIGIPEVSPGDDLAGLLAAAATGSRIGLDDGD
                     VLVVSSKVVSKALGLRSPDEDRESVVRQESRYVVAERRAGEVTTRIVRASAGPVMAAA
                     GVDASNTGGDPVVLRLPHDPDGVCRDLRERLRTRHDAGRIAVVLTDTAGRPWRIGQTD
                     FALGAAGLRCVDDLRGRLDADGRPLHVTSRAVADELAAAADLVKGKTARCPAVVVRGL
                     AHLVEDDDGPGATALLRVGDGDWFGYGAAEAVRASLGVEPGSAAAQVVGVAATGPEPL
                     PD"
BASE COUNT          323 a          955 c          922 g          377 t
ORIGIN      
        1 gttgtgggcg acgaggacgg caagaacggt cggggccgcc cgcggcgacc cgggctcggg
       61 ggtgggcccc tcggactggg catggtcgag ctccgcgccg agcggcgcgg cagcggagtc
      121 cagggaggga gtcgtcacgg cacgatccga cgtcgtagtg acgtcatggt cggcgcgggt
      181 cgccggagcg actagaccgc gcgcttcttg agcttgcgtc gttcacgctc ggacagcccg
      241 ccccagatgc cgaagcgctc atcgttggcc agggcgtact cgaggcactc cgcgcgcacg
      301 tcacagccga ggcagacctt cttggcctcc ctggtcgagc cgcccttctc ggggaagaac
      361 gcctccggat cggtctgcgc gcacagcgag cgctcctgcc acgagagtgc ctcgacgggc
      421 tctccctcgc tcgtgaccac tctgagttcc agcacggttc ctcctcgacc caaatcgtgc
      481 ttcggcgctg ccgtcccgtt ccagccgtgg ggctctcccc gccgcttcgg tcgacgaccc
      541 cgcgaaggtc actgaaagac aacaatggaa ttacacgcgt gtcataccgg gttagtcaag
      601 cgatcttctg gtatttgtcc aagtcctgac gaacgtcatc gagcgccggg agccgcgcag
      661 accatgggcc ggcgcctgag agactgtctc catgagcgtg cccgacccct cccctggcct
      721 gcggatcacg gcacttgccg ggggcgtcgg aggtgcccgc ttcctgcgcg gcctgcttgc
      781 ctacctggcc gagaccaccg acctggccgg cagcaccgtg accgtcatcg gcaacaccgg
      841 ggacgacatc accttgttcg gcctgcgcgt gtgcccggac ctcgacactc tcgtctacac
      901 cctcggcggc ggcgtccacg aggggcaggg gtggggacgc gcggacgaga cgtacgccgt
      961 ccaaggtgag ctctcggcat acggcgtcgg tccgcagtgg ttcgggctgg gtgaccggga
     1021 cttcggcacc cacgtggccc gctcgctctg gctcggccag ggactgaccc tgagcgaggt
     1081 gaccgcccgg ctggccacgc ggtggggact gcccgagcgc ggcgtcacgc tgctgcccat
     1141 gaccgacatg cccgtcgaga cccacgtcgt cgtcgaggac gagcaggggc ggcgcgcggt
     1201 gcacttccag gagtggtggg tccggatgcg ggccgcggtc ccggcgcagc gcttcgtcgt
     1261 ggcgggcatg gaccgcgccg acgccgcacc gggggtcctc gacgcgatcc ggcaggccga
     1321 cctggtgatc cttcccccga gcaaccccgt ggtgtccatc gggatcatcc tcggggtgcc
     1381 gggagtgcgg gacgccctgc gcggcacccg cgctccggtc gtcggggtct caccgctgat
     1441 cgacgggcgc ccggttcgcg gtcacgccga tgcctgcctg cgtgcgatcg gcatcgagtc
     1501 gacctcggcg gccgtggccg ggctctacgc cgacttcctc gacggttggc tcgtcgactc
     1561 cctggacacc gccggctcca tcgaccagac cgaccccacc cacgcccgtc gcgccgcccg
     1621 gggcgcccgg ccggcggtgc gcccgcgccc cctcctcatg gtcgacctga cctcggccgc
     1681 cgcgatcgcc ggggcagccg tcgagctggg gcgcgagctc ggcgcccgtc cggccgcgtg
     1741 agcatgccct ccgaccccgc cccgctggag atcctgccgc tgatcggcat ccccgaggtg
     1801 agccctggcg acgacctggc cggcctgctc gccgcggcgg ccaccggctc gcggatcggc
     1861 ctcgacgacg gtgacgtcct cgtcgtctcg agcaaggtcg tgtccaaggc gctcgggctg
     1921 cgctcgcctg acgaggacag ggagtcggtc gtgcgtcagg agagccggta tgtcgtcgcc
     1981 gagcgccgcg cgggcgaggt caccacacgc atcgtgcggg ccagcgccgg cccggtgatg
     2041 gctgcggccg gggtcgacgc ctcgaacacg ggcggtgacc ctgtcgtgct gcggcttccc
     2101 cacgacccgg acggtgtgtg ccgcgacctg cgcgagcggc tgcgcacccg gcacgacgcg
     2161 gggcgtatcg ccgtcgtgct caccgacacg gccggacgac cgtggcgcat cgggcagacc
     2221 gacttcgccc tgggagcggc cgggctccgg tgtgtggacg acctccgcgg ccggctcgac
     2281 gccgacgggc gcccattgca cgtcacctcc cgcgccgtgg ccgacgagct cgccgccgcg
     2341 gccgacctcg tcaagggcaa gaccgcccgc tgtccggcag tcgtcgtccg cggtctggcg
     2401 cacctggtcg aggacgacga cggccccggc gcgaccgccc tgctgcgcgt cggtgacggc
     2461 gactggttcg gctatggcgc ggccgaagcg gtgcgggcca gtctcggtgt cgagcctggc
     2521 tccgccgccg cgcaggtggt cggggtcgcg gcgaccggcc ccgaacccct gcctgac
//
LOCUS       DATAIX010000529         4391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_28337, whole genome shotgun sequence.
ACCESSION   DATAIX010000529 DATAIX010000000
VERSION     DATAIX010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28337"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VHM65_07895"
     CDS             complement(<1..336)
                     /locus_tag="VHM65_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX2705665.1"
                     /translation="MDGVDATEPLRLLLRDLRSDPRGLSEREAARRLVAFGPNELRRT
                     GRRHPLKELVDQLVHPLALLLWVAAGLSVITRTPALSVAIVLVICLNAAFAFVQERQG
                     EQAVEALSQY"
     gene            438..1502
                     /locus_tag="VHM65_07900"
     CDS             438..1502
                     /locus_tag="VHM65_07900"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2705666.1"
                     /translation="MTFPSALPVSRVGDSMEAPPLRWGILGPGSIADRFAAALTTRTR
                     QTVQAVGSRTLGAATAFAERRGVPRAYGSYDALVADPDVDIVYVATPHNFHRPHALLA
                     LQAGKHVLVEKPMTLNVSEAREVAALARAKGLFAMEALWTLFLPKFDVLRQLLADGAV
                     GDVRTVLADHGQYFEAGHRILRPDLAGGPMLDLMTYPASFAHWVIGAPTEVRALTTWV
                     PSGVTGQTAIMYAAAGDRQALLHSSVLGPTPTTGSIGGSTGHLFLDGDFYTPGGFTLT
                     SRTGRRLRYDEPPVGHEALHFQAAEAARRIDAGEVESPVRPLQATIETMAIMDEARRQ
                     IGERFAEEGASPEGPEESRS"
     gene            1505..2326
                     /locus_tag="VHM65_07905"
     CDS             1505..2326
                     /locus_tag="VHM65_07905"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2705667.1"
                     /translation="MTTATDWMYDGAHGALAAHTWRGEQTPRYVAVLCHGYGEYVGRY
                     EHVADALVQDGAVVYGVDHQGHGHSDGERVLVADIESVVDDVDGLVSRARSEWPGLPV
                     ALIGHSMGGLVAARYAQRFGAKLAVLVLSGPVIGRMDLVPALLSMDEIPDIPIDPATL
                     SRDDRVGAAYAADPLVWHGPFKRPTLLALQAGIDAVNAAGSLGALPLLWIHGELDQLV
                     PIDGSREGVERIRGLTFEQRIYEGARHEVFNETNQDEVIGDVIAFVNRALDRASS"
     gene            2368..3387
                     /locus_tag="VHM65_07910"
     CDS             2368..3387
                     /locus_tag="VHM65_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705668.1"
                     /translation="MFGSAMVDTAIGLSFVFALAALLCTGLVELGATALRKKGKYLLR
                     GLNDLLGAADSTVTGWRESVTMVTAGASLERPMYAGALEAGPASAPTQGATSLSLAHI
                     MGHPLVDVLKSTSASGEKVRNPAYLSAQTFSAVLVDLLVPASGGHELADLKTAVQAMT
                     GNPTLKRSLSTLLTTAGDLATFRAGLERWYDEHMDRVGAAYTRWARRWCVVIALGLAG
                     VFGIDALAIGTTLYTDPVVRQTVVAAATADGASALCGTSTTLEDRTACVDKALAKLGG
                     AGIPLGWGEQNRPADPIGWVTKLFGIVLTALAARLGAPFWFNAIGRLGVVRRTSGDRP
                     AEAAG"
     gene            3384..>4391
                     /locus_tag="VHM65_07915"
     CDS             3384..>4391
                     /locus_tag="VHM65_07915"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2705669.1"
                     /translation="MRTRHHDLVVIGTGSGNSVLTPELAHLDIAVVESGRFGGTCLNV
                     GCIPTKMYVYPADLVVEARRATALGVRFADPEVSWDAIRDRVFGRIDPIAAEGESHRR
                     TQERVTVYGGHARFVGERRLAVDLAGGSSVTIEGERVVIAAGGHAVGLDLDGLRGADP
                     ERGVHTSDTVMRMRSLPGRMAVIGGGFVAAEFAHVFDAFGVAVTHLHRGARLLKAEDE
                     EVAQRYTEIVGRRYDLRLGSRIVSAQRRDDGVWALTVAGPSRTCMLEVDAVLLAVGRR
                     SNAPALDVAAGGVAQHPDGRVVVDGLQRTSVPGVYALGDVSSDYELKHVANHEARVVA
                     HN"
BASE COUNT          615 a         1569 c         1591 g          616 t
ORIGIN      
        1 gtactggctc agggcctcca ccgcttgctc cccttggcgc tcctggacga acgcgaaggc
       61 cgcgttgaga cagatcacga gcacgatggc gaccgacagg gccggtgtcc gggtgatgac
      121 ggacagcccg gcagccaccc acaggaggag cgccaagggg tgcaccagct ggtcgacgag
      181 ctccttgagc ggatgccgcc ggccggtccg gcgcagctcg ttcggcccga aggccacgag
      241 acgacgagca gcctcgcgtt cggagagtcc acgcgggtcg ctacgcaggt cccgaaggag
      301 cagccgcaac ggctccgtgg catcgacacc gtccatgacc accctcgtca cgctaccccc
      361 gcccggaacg ggcgtgtcgc ccaggacacg gcgggacagg ccgtgggggt gaggtgcggc
      421 gtcatacgct ggccggtatg acgttcccct cggctctccc ggtgtcccgc gtcggcgact
      481 ccatggaggc tccgccgctg cggtggggca tcctcgggcc ggggtcgatc gccgacaggt
      541 tcgccgctgc gctgacgacc cgcacgcggc agacggtcca ggccgtgggg tcgcgaacac
      601 tcggcgcggc aacggctttc gccgaacgcc ggggggtgcc cagggcatac ggctcgtacg
      661 acgcgctcgt ggccgacccg gacgtcgaca tcgtctatgt cgcgacgccg cacaacttcc
      721 accggccgca cgccctgctc gcgctccagg ccggcaagca tgtcctcgtc gagaagccca
      781 tgaccctcaa cgtgtccgag gcccgtgagg tggcggcgct ggcccgggcc aaggggctct
      841 tcgccatgga ggcgctctgg acgctgttcc tgcccaagtt cgacgtcctg cgccagctgc
      901 tcgccgacgg ggccgtgggc gatgtgcgga ccgtgctcgc tgaccacggc cagtacttcg
      961 aggcgggcca ccggatcctt cgccccgacc tggccggcgg gccgatgctc gacctcatga
     1021 cctaccccgc gtcgttcgcc cactgggtca tcggcgcgcc caccgaggtg cgggcgctga
     1081 ccacctgggt ccccagtggc gtcaccgggc agacggcgat catgtatgcc gccgcgggcg
     1141 accgtcaggc gctgctgcac tccagcgtcc tcgggccgac gccgacgacc ggctcgatcg
     1201 gcggcagtac cgggcacctc ttcctcgacg gcgacttcta cacgccgggc gggttcacgc
     1261 tcacctcgcg cacgggccgg cggctgcgct acgacgagcc gccggtcggc cacgaggcgc
     1321 tgcacttcca ggccgccgag gccgcgcggc gcatcgacgc gggtgaggtc gagtcgccgg
     1381 tccggccgct gcaggccacg atcgagacga tggcgatcat ggacgaggcc cgccgccaga
     1441 tcggcgagcg ctttgccgag gaaggggctt cccccgaggg gcccgaggag agcaggagct
     1501 gacgatgacg acagcgaccg actggatgta tgacggagcc cacggcgctc tcgccgcgca
     1561 cacctggcgt ggcgagcaga ccccgaggta cgtcgcggtg ctgtgccacg gctacggcga
     1621 gtacgtcggg cgctacgagc acgtggcgga cgcactcgtg caggacggtg ccgtcgtcta
     1681 cggggtcgac caccagggcc acgggcacag tgacggcgaa cgggtgctcg tcgccgacat
     1741 cgagtcggtc gtcgacgacg tcgacgggct cgtgtcgcgg gcgcgctcgg agtggccggg
     1801 actgccggtc gcgctcatcg ggcactccat gggcgggctc gtcgcggccc gctatgccca
     1861 gcggttcggt gccaagctcg ccgtcctcgt cctgtccggc ccggtgatcg ggcggatgga
     1921 cctcgtaccg gccctgctgt cgatggacga gatccccgac atcccgatcg accctgcgac
     1981 gttgtcgcgc gacgaccggg tgggtgcggc ctatgccgcc gacccgctgg tgtggcacgg
     2041 gccgttcaaa cgcccgaccc tgctcgcgct ccaggccggg atcgacgcgg tcaacgccgc
     2101 cggaagcctc ggcgcgctgc ccctgctgtg gatccacggg gagctcgacc agctcgtgcc
     2161 gatcgacggc agccgggaag gcgtcgagcg gatccgcggc ctcaccttcg agcagcggat
     2221 ctacgagggc gctcgccacg aggtgttcaa cgagaccaac caggacgagg tcatcggcga
     2281 cgtcatcgcc ttcgtcaacc gcgccctcga ccgcgcgtcg tcgtagccac ccacggcccc
     2341 gccgtcgggt tcgcaggagg agggaccatg ttcggaagcg caatggtcga caccgcgatc
     2401 ggcctgtcgt tcgtcttcgc tctcgccgcg ttgctgtgca cgggactcgt cgagctcggc
     2461 gccaccgccc tgcgcaagaa gggcaagtac ctcctccgcg ggctcaacga cctcctgggc
     2521 gcggcggact cgaccgtgac cgggtggcgg gagagcgtca ccatggtcac tgccggagcc
     2581 agcctcgaac ggccgatgta tgccggcgcg ctcgaggcgg gcccggcgtc ggcaccgacc
     2641 cagggcgcga cctcgctctc gcttgcgcac atcatggggc accccctcgt cgacgtgctc
     2701 aagtcgacct cggcgagcgg cgagaaggtg cgcaacccgg catacctctc ggcgcagacc
     2761 ttctctgcgg tcctcgtcga cctgctcgtg ccggcgtcgg ggggccacga gctggccgac
     2821 ctcaagaccg ccgtccaggc catgaccgga aacccgaccc tcaaacggtc cctgagcacg
     2881 ctgctcacga cggccgggga cctcgcgacg ttccgggccg gcctggagag gtggtacgac
     2941 gagcacatgg accgggtcgg ggcggcctac acccgatggg cccgccggtg gtgcgtcgtc
     3001 atcgcgctgg ggctggccgg cgtcttcggg atcgacgcgc tggccatcgg gaccacgctc
     3061 tacaccgatc cggtcgtgcg ccagaccgtc gtggcggcgg ccacggccga cggggcatcg
     3121 gccctgtgcg gcacgtcgac gacgctcgag gaccgcaccg cctgtgtcga caaggcgctg
     3181 gccaagctgg gtggcgccgg catcccgctc ggctggggcg agcagaaccg gccagccgac
     3241 ccgatcggct gggtcaccaa gctcttcggc atcgtgctca ccgccctggc cgcccgcctg
     3301 ggggcaccgt tctggttcaa cgccatcggg cggctcgggg tggtgcgcag gacgtccggc
     3361 gaccgccccg ccgaggccgc cggatgagga cacgccacca cgacctcgtc gtcatcggca
     3421 cggggtcggg caactcggtg ctcacgcctg agctggcgca cctcgacatc gcggtcgtcg
     3481 agtcgggccg cttcggcggg acctgcctca acgtcggctg catcccgacc aagatgtacg
     3541 tctacccggc cgatctcgtc gtcgaggccc gccgcgccac cgcgctcggg gtccggttcg
     3601 ccgaccctga ggtgagctgg gacgccatac gggacagggt ttttgggcga atcgacccga
     3661 ttgcggccga gggggagtcg caccgccgta cccaggagcg agtgacggtg tatggcgggc
     3721 acgcgcggtt cgtcggggaa cgacggctgg cggtcgacct ggccggcggg tccagcgtca
     3781 ccatcgaggg cgagcgggtc gtcatcgccg ccggtgggca cgcggtgggg ctcgatctcg
     3841 acgggctgcg cggcgccgac ccggagcggg gggtgcacac gtccgacacg gtgatgcgga
     3901 tgaggtccct gccggggcgg atggcggtca tcgggggcgg tttcgtcgcg gccgagttcg
     3961 cccacgtgtt cgacgccttc ggggtggccg tgacccacct ccaccggggc gcgcggctgc
     4021 tcaaggccga ggacgaggag gtggcccagc gctataccga gatcgtgggg cggcgctacg
     4081 acctgcgcct ggggtcccgg atcgtgtcgg cccagcgcag ggacgacggg gtctgggcgc
     4141 tcaccgtcgc ggggccgtcg cggacctgca tgctcgaggt cgacgccgtg ctgctcgcgg
     4201 tcggccggcg ctccaacgcg cccgcactcg atgtggccgc gggcggggtg gcccagcacc
     4261 cggacgggcg ggtcgtcgtc gacggcctac agcggacgtc ggtgccgggc gtctacgccc
     4321 tgggcgatgt cagcagcgac tatgagctca aacacgtcgc caaccacgag gcccgggtcg
     4381 tcgcccacaa c
//
LOCUS       DATAIX010000530         4543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_250833, whole genome shotgun sequence.
ACCESSION   DATAIX010000530 DATAIX010000000
VERSION     DATAIX010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250833"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2195)
                     /locus_tag="VHM65_07920"
     CDS             complement(<1..2195)
                     /locus_tag="VHM65_07920"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX2705670.1"
                     /translation="MRLVRRIGLAITVVLVVLLVAAGVVGVTTVRASWPETTGTISLP
                     SLTGTVRVLRDARGVPHIYAGNTADLMRAQGFVSAQDRFFEMDLRRHVTAGRLSELVG
                     DAGVATDKVIRTLGWRRIAEQELPKLSADTRQNLDAYAEGVNDYLRSHTEVRAISLEY
                     TILGMRYGGYTPEPWSALDSVAWLKAMAWDLKGDYGNELARATLAPSVSAAQLALLYP
                     PYPYDAHKPILSAQDWTPQSVQSGLARPVPALLRNPSLAATWSSVRAAVDAVPVTLGR
                     GEGIGSNSWVVGPSKSTTGAPQLANDPHLGVGIPGIWMQVGLHCTTVSDACPFDVSGF
                     SFSGVPGIVIGHNDHIAWSFTNLGPDVSDFYLEKVSGDTYLYDGKQVPLVTRTETIKV
                     AGGTDVTITVRSTGHGPILSDVISSVADMGRKAPVNAKIAKESYAVSLAWTGLRPGAT
                     ADAIFGLNLARDFTDFRTAAELFAVPSQNLLYADREGHIGYQAPGLVPIRGSSVNGAP
                     PGYWPAPGWDSTYDWKGFLPFEALPFTFDPPEGVIVAANQAVTGEASPFLTTEWDYGF
                     RAQRIRTELTATAKVSPEKMAEIQLDTRNGFAPTLAKALLAIDLRSDPFTRSGQNLLR
                     RWDYTQPAGRSSASAAASYYNAVWRRLLLYTFEELPADLGPDGGARWMAAVGGLLREP
                     TNAWWDDRNTRGVVEGEQEILRKALVQARLDLTEQLGKEPATWIWGRLHS"
     gene            2328..3845
                     /locus_tag="VHM65_07925"
     CDS             2328..3845
                     /locus_tag="VHM65_07925"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEX2705671.1"
                     /translation="MPYDVLAAPVFGVDDLTTVLLIGAAVLIASIVAVRLSVHSGLPS
                     LLYYLAIGLLIGEAGFGIRFDSANLTRVLGYAALILILAEGGLTTRWSSVRQSVAPAA
                     VLSTVGVAVSVSVVAVAAHVVLHVSWTVALLIGAIVSSTDAAAVFSVLRNVPLPRRIT
                     GLLEAESGINDAPVVILVVTLAAQAMPGAEPEPWYLLVVLAALELVGGALVGLAVGHL
                     GARLMKRFAARTSGLFSIGIVALTVLAYAVAASAHTSGFLATYVSGVVLGNMRLPHRV
                     AVHGFATALGWLAQIGLFVLLGLLASPARMPGQIVPALVIGLVLLLVARPLSVLVSVT
                     PFRVPWRDQVFLSWAGLRGAVPVVLATVPLTMGTPDTGWLFDLVFVLVIVFTVVQAPT
                     LPWVARRLGVIEADAATDVELESTLLDELGAEVVQVSIGLASRLNGVGVVELRLPTGA
                     QVALVARAGTTFVPDRMAQFRHGDRLLIVTTAAARSAVEARVRAVSRDGRLAGWV"
     gene            3999..4415
                     /locus_tag="VHM65_07930"
     CDS             3999..4415
                     /locus_tag="VHM65_07930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX2705672.1"
                     /translation="MPTYAYACTLCGHTFDHVQSFHEDALSVCPECSGRLRKVYGSIG
                     VSFKGSGFYRNDSRADAKRPAAAASSPGSGSSDSGSGSSGSGSGSSGSESSGPSGSGS
                     SGGTDSGRSSSGGSAGSSGSGRGRSGASTAAAASAS"
BASE COUNT          548 a         1595 c         1682 g          718 t
ORIGIN      
        1 gagtgcagcc ggccccagat ccacgtcgcg ggctccttgc cgagctgctc ggtcaggtcg
       61 agccgcgcct ggacgagagc cttgcgcagg atctcctgct ccccctcgac gacgccgcgg
      121 gtgttgcggt cgtcccacca cgcgttggtc ggctcgcgca gcaggccccc gacggccgcc
      181 atccagcggg ccccgccgtc gggtccgagg tcggcgggaa gctcctcgaa cgtgtagagc
      241 aggaggcggc gccataccgc gttgtagtag ctcgccgccg ccgacgccga ggagcgtccc
      301 gcgggctggg tgtagtccca ccggcgcagc aggttctgac ctgacctggt gaaggggtca
      361 cttcgcaggt cgatggcgag cagggccttg gccagggtcg gggcgaaccc gttgcgggtg
      421 tcgagctgga tctcggccat cttctcgggc gagaccttgg ccgttgcggt gagctcggtg
      481 cggatgcgct gcgcgcggaa gccgtagtcc cactcggtcg tgaggaacgg gctggcttcg
      541 cccgtcaccg cctggttggc cgcgacgatg accccctcgg gcgggtcgaa cgtgaacggc
      601 agcgcctcga agggcaagaa acccttccag tcgtaggtgc tgtcccagcc gggcgccggc
      661 cagtagccgg gcggggcgcc gttgaccgag ctgccccgga tcgggaccag acccggcgcc
      721 tggtagccga tgtgcccctc acggtcggcg tagagcaggt tctgcgacgg caccgcgaac
      781 agctccgccg cggtgcggaa gtcggtgaag tcccgggcca ggttgagccc gaagatcgca
      841 tcggccgtcg ccccgggccg caggccggtc caggccaggg acacggcata cgactccttg
      901 gcgatcttgg cgttgacggg ggccttgcgc cccatgtcgg cgaccgagga gatgacgtcg
      961 gacaggatcg ggccgtggcc ggtcgaccgt acggtgatcg tgacgtccgt gccaccggcg
     1021 accttgatcg tctcggtgcg ggtcacgagc gggacctgct tgccgtcata gaggtaggtg
     1081 tcgccgctga ccttctcgag gtagaagtcg ctcacgtcgg gaccgaggtt ggtgaaggac
     1141 caggcgatgt ggtcgttgtg cccgatgacg atgcccggca cgccggagaa gctgaagccg
     1201 ctcacgtcga aggggcacgc gtccgagacc gtcgtgcagt gcagcccgac ctgcatccag
     1261 atgccgggga ttccgacgcc gaggtgcggg tcgttggcca gctgcggcgc cccggtcgtc
     1321 gacttcgacg ggccgacgac ccacgagttc gagccgatgc cctccccgcg cccgagggtg
     1381 accggcaccg cgtcgaccgc cgcccgcacc gaggaccagg tcgcggccag cgaggggttg
     1441 cgcagcaggg ccgggacggg gcgcgcgagc ccgctctgga ccgactgggg ggtccagtcc
     1501 tgggccgaca ggatgggctt gtgcgcgtca tacgggtatg gcgggtagag cagcgccagc
     1561 tgggcggccg agaccgaggg ggccagcgtg gcgcgggcca gctcgttgcc gtagtcgccc
     1621 ttgaggtccc aggccatggc cttgagccag gcgaccgagt ccagcgccga ccacggctcc
     1681 ggggtgtagc cgccgtagcg catcccgagg atggtgtact ccagggagat ggcgcggacc
     1741 tcggtgtggg acctgagata gtcgttgacg ccctcggcat aggcgtcgag gttctggcgg
     1801 gtgtcggccg agagcttggg cagctcctgc tcggcgatgc gccgccagcc gagggtgcgg
     1861 atcaccttgt ccgtcgcaac cccggcgtcg ccgacgagct cggacaggcg ccccgcggtg
     1921 acgtgccggc gcaggtccat ctcgaagaac cggtcctgcg cggagacgaa cccctgggcc
     1981 cgcatgaggt cggcggtgtt gccggcgtag atgtgcggca caccccgggc gtcgcgcagg
     2041 acccggaccg tgccggtgag gctcggcagc gagatcgtcc cggtcgtctc gggccacgag
     2101 gcccggacgg tggtcacgcc gacgaccccc gcggcgacga gcaggacgac gagcacgacg
     2161 gtgatggcca ggccgatccg acggacgagt cgcatgcgct gcaccctgcg agactaggac
     2221 acgccgaacg ccggacggct cagcgtggcc gactcgatgc cgtattggcg cctgctgctc
     2281 ccggcgagcg gtaggaggac gagtgagcgg ggaggaggtg accggggatg ccgtatgacg
     2341 tgctggccgc tcccgtcttc ggggtcgacg acctcacgac cgtcctgctc atcggggcag
     2401 cggtgctcat cgcgtccatc gtggccgtcc ggctctccgt ccactcgggc ctgccgtcac
     2461 tgctctacta cctggccatc gggctgctca tcggagaggc cggcttcggg atccgcttcg
     2521 actcggcgaa cctgacccgc gtcctcggct acgccgccct gatcctcatc ctcgccgagg
     2581 gcgggctcac gacccgctgg tcgtcggtcc gccagtcggt ggctccggcg gcggtgctgt
     2641 cgaccgtggg ggtcgccgtg tcggtcagcg tggtggccgt cgccgcccat gtcgtcctcc
     2701 acgtgagctg gaccgtggcc ctgctcatcg gagccatcgt gtcctcgacc gatgccgccg
     2761 cggtcttctc cgtcctgcgc aacgtcccgc tccctcggcg gatcaccggg ctgctcgagg
     2821 ccgagtccgg catcaacgac gccccggtcg tgatcctcgt cgtcacgctg gccgcccagg
     2881 ccatgccagg cgcggagccc gagccgtggt acctcctggt ggtgctcgcg gccctcgagc
     2941 tggtcggcgg tgctctcgtc ggcctcgctg tcggtcacct cggggcgcgc ctcatgaagc
     3001 gcttcgcggc ccggacctcg ggcctgttct cgatcgggat cgtcgcgctg accgtcctcg
     3061 cgtatgccgt cgcggcgagc gcccacacga gtggcttcct cgcgacgtac gtcagcgggg
     3121 tggtgctggg caacatgcgc ctgccgcacc gcgtggccgt ccacgggttc gccaccgccc
     3181 tcgggtggct cgcgcagatc gggctgttcg tgctcctcgg gctgctcgcc tccccggccc
     3241 ggatgccggg ccagatcgtc ccggcgctgg tcatcgggct cgtcctgttg ctcgtcgccc
     3301 ggccgctgtc ggtcctcgtg tcggtgacgc ccttccgggt gccgtggcgc gaccaggtct
     3361 tcctgtcctg ggcggggctg cgcggggcgg tgccggtcgt gctcgcgacg gtgccgctca
     3421 cgatggggac acccgacacg ggctggctct tcgacctcgt cttcgtgctc gtcatcgtct
     3481 tcaccgtggt ccaggccccg acactgccct gggtggcgcg ccggctcggg gtgatcgagg
     3541 cggatgcggc gaccgacgtc gagctggagt cgacgctgct cgacgagctg ggcgccgaag
     3601 tggtccaggt cagcatcggc ctcgcctcgc gcctgaacgg ggtcggggtc gtcgagctgc
     3661 gcctgcccac cggcgcgcag gtggccctgg tcgcgcgggc cgggacgacg ttcgtgcccg
     3721 accggatggc ccagttccgc cacggggacc ggctgctcat cgtgacgacg gcagcggccc
     3781 ggtcggcggt cgaggcccgg gtccgagcgg tgagccgcga cgggcgcctg gcgggctggg
     3841 tctaggtcgt cgcgggccgg cgcgggtgct gcgccggccc ggcataccgg attcggacgg
     3901 ctcgccacgg ggacatagac tgcgacatag gcagcggggg gcccctgcgc gctcggcgcc
     3961 gggccctggt cgttgccggt ttcccgatgg gaggacccgt gcccacgtac gcctacgcgt
     4021 gcaccctgtg cggccacacc ttcgaccacg tccagtcgtt ccacgaggac gcgctgtcgg
     4081 tctgccccga gtgctcgggg cggctgcgca aggtctacgg ctcgatcggc gtgtccttca
     4141 agggatccgg cttctaccgc aacgactcgc gcgccgacgc caagcgcccg gcggctgcgg
     4201 cgtcctcgcc gggctcgggg tcctccgact cgggctcggg ctcctccggc tcgggctcgg
     4261 gctcctccgg ctcggaatcg tccggcccct ccggctcggg gtcctccggt ggcaccgact
     4321 cggggcgttc gagctcgggt ggttcggcag gctcctccgg gtcgggccgg ggccgctcgg
     4381 gggccagcac ggccgccgca gccagcgcct cgtaggcccg ctgtccacaa cccctgctcc
     4441 agtcgtccca ctgtccaccg agccgggggc tcgccggggt cggctgagcc aggtggccct
     4501 aacgtcgtcg acgtgagctt caccgtgtct gccccgcccg agg
//
LOCUS       DATAIX010000531         2780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_118275, whole genome shotgun sequence.
ACCESSION   DATAIX010000531 DATAIX010000000
VERSION     DATAIX010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118275"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VHM65_07935"
     CDS             <1..959
                     /locus_tag="VHM65_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX2705673.1"
                     /translation="MERSFPRLDVHVRGNEISLRGPGAEVAIAERLIDELIVIIGAGQ
                     PLNRDAVERSIGMLRAQTRERPADVLTMNIVSSRGRTIRPKTLNQKRYVDAIDEHTVV
                     FAIGPAGTGKTYLAMAKAVAALQAKAVSRIILTRPAVEAGERLGFLPGTLNDKIDPYL
                     RPLYDALHDMIDPDSIPRLMAAGTIEVAPLAYMRGRTLNDAFIILDEAQNTSSEQMKM
                     FLTRLGFGSKIVVTGDVTQVDLPGGTTSGLRHVRTILDGMDDIHFAELTSGDVVRHRL
                     VAAIVDAYDAADSRERRTDEGHADLRDRRRGAARSARAGGAR"
     gene            956..1423
                     /gene="ybeY"
                     /locus_tag="VHM65_07940"
     CDS             956..1423
                     /gene="ybeY"
                     /locus_tag="VHM65_07940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX2705674.1"
                     /translation="MSVDVLNETDADLDELELLACARYVMAQMRVHPQADLCIKLVDE
                     AAMEVLHVQWMDLPGPTDVMSFPMDELRPARDGHEPEEGVLGDIVLCPAVAARQAAEA
                     GHAPEEELLLLTTHGILHLLGYDHGEPGQEIEMFELQRQLLLTFLAGRGRPGA"
     gene            1435..>2780
                     /locus_tag="VHM65_07945"
     CDS             1435..>2780
                     /locus_tag="VHM65_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX2705675.1"
                     /translation="MNGALLFAAVLATIVAFCFATSEAAILRMSRVRAAELLEDGRSG
                     AESLVQVVSDTAPYLAVITFVRVIAEATTAVVITLAVYARLQDLRTSLLVSVAVMAVV
                     SFVVVGVSPRTLGAQNYVRVALVSAPLAVGLRSVLGPVAQLLVLVGNMVTPGKGFRDG
                     PFRSESELRDLVDMAGESSVIEHGEREMIQSVFDLGDTIAREVMVPRTDMVTIAHDKN
                     LRQANSLFLRSGFSRIPVAGEDSDDILGLLYLKDVLLQTSSDPAAASKLVESVMRPMS
                     FVPDSKPVDDLLREMQREQSHFAIVVDEYGGTAGLVTIEDILEEIVGEIADEHDREAP
                     GVEVMANGTTRVPAQMHIDDLADLFDVRIDEEEVDTVGGLLAKLVGRVPIPGSSARIA
                     GLTLRAERMAGRRHRLATVIVSVGAAAAGKGIPAAADVARVSRTAESASASADSPST"
BASE COUNT          424 a         1014 c          932 g          410 t
ORIGIN      
        1 cgatggagcg gtccttcccg cggctcgacg tgcacgtccg gggcaacgag atctccctgc
       61 gggggccggg cgccgaggtc gcgatcgccg agcggctcat cgacgagctc atcgtcatca
      121 tcggcgcggg ccagccgctc aaccgtgacg ccgtcgagcg ctcgatcggc atgctccggg
      181 ctcagacccg cgagcggccc gctgacgtcc tgacgatgaa catcgtgtcc agccgtgggc
      241 gcacgatccg tcccaagacg ctgaaccaga agcggtatgt cgatgcgatc gacgagcaca
      301 ccgtggtgtt cgccatcggc cccgccggga cgggcaagac ctacctcgcg atggccaagg
      361 cagtcgcggc cctgcaggcc aaggccgtct cgcggatcat cctgacccgg ccggccgtcg
      421 aggcggggga gcggctcggc ttcctcccgg ggacgctcaa cgacaagatc gacccgtatc
      481 tgcggccctt gtacgatgcg ctgcacgaca tgatcgaccc cgactcgatc ccaaggctca
      541 tggccgccgg gacgatcgag gtcgccccgc tggcctacat gcgcggccgc accctcaacg
      601 acgcgttcat catcctcgac gaggcccaga acacctcgag cgagcagatg aagatgttcc
      661 tgacccggct cgggttcggg tccaagatcg tcgtcaccgg ggatgtcacc caggtcgacc
      721 tgccaggcgg cacgacctcg ggccttcggc acgtccgcac catcctcgac gggatggacg
      781 acatccactt cgccgagctc acctcgggtg acgtcgtgcg ccaccgcctc gtcgcggcga
      841 tcgtcgacgc gtatgacgcc gccgacagcc gcgagcgccg caccgacgaa gggcacgccg
      901 acctgcggga ccggcgccgc ggcgcggccc ggtcagcccg ggccggaggc gcccgatgag
      961 cgtcgacgtc ctcaacgaga ccgacgccga cctcgacgag ctcgagctcc tcgcctgtgc
     1021 ccgctacgtc atggcccaga tgcgcgtcca tccgcaggcc gacctgtgca tcaagctcgt
     1081 cgacgaggcc gcgatggagg tcctgcacgt ccagtggatg gacctgcccg gcccgaccga
     1141 cgtgatgagc ttcccgatgg acgagctgcg ccccgcccgc gacgggcacg aacccgagga
     1201 aggggtcctc ggcgacatcg tcctgtgccc ggcggtggcc gcgcgccagg ccgccgaggc
     1261 cggacacgcc ccagaggagg agctgctcct gctcaccacc cacggcatct tgcacctgct
     1321 cggctacgac cacggcgaac ccgggcagga gatcgagatg ttcgagctgc agcgccagct
     1381 cctgctgacg ttcctcgccg gccgcggccg gcccggcgcc tgacgggccc ggagatgaac
     1441 ggagcgctcc tcttcgccgc ggtcctggcc acgatcgtgg ccttctgctt tgccacgagc
     1501 gaggcggcga tcctgcggat gtctcgcgtc cgcgccgccg agctgctcga ggacggccgt
     1561 agtggcgcgg agtcgctcgt ccaggtcgtg agcgacaccg cgccatacct ggccgtcatc
     1621 accttcgtgc gggtcatcgc ggaggcaacc acagcagtcg tcatcaccct cgccgtgtac
     1681 gcgcgcctgc aggacctgag gacctcgctg ctcgtcagcg tcgccgtcat ggccgtcgtc
     1741 tcgttcgtcg tcgtcggtgt gtcgccgcgc acgctcggtg ctcagaacta cgtccgggta
     1801 gccctcgtct cggcgccgct cgcggtcggg ctgcgctcgg tgctcggccc ggtggcgcag
     1861 ctcctcgtcc tggtcggcaa catggtgaca ccgggcaagg gcttccggga cgggccgttc
     1921 cggagcgagt ccgagctgcg cgacctcgtc gatatggctg gggagtcctc agtcatcgag
     1981 cacggcgaac gcgagatgat ccagtcggtc ttcgacctcg gcgacacgat cgcccgtgag
     2041 gtcatggtcc cccgcaccga catggtgacg atcgcgcacg acaagaacct gcgtcaggcc
     2101 aactcgctct tcctgcgctc cggcttctcc cggatcccgg tcgccggcga ggactcggac
     2161 gacatcctcg gtctgctcta cctcaaggac gtcctgctgc agacgagctc ggacccggcc
     2221 gccgcgagca agctcgtcga gtcggtcatg cgaccgatgt ccttcgtccc ggactccaag
     2281 ccggtcgacg acctcctgcg cgagatgcag cgcgaacaga gccacttcgc gatcgtcgtc
     2341 gacgagtatg gcgggaccgc cggactcgtc acgatcgagg acatcctcga ggagatcgtc
     2401 ggcgagatcg cggacgagca cgaccgggag gccccgggag tcgaggtcat ggccaacggg
     2461 acgacgcggg ttccggccca gatgcacatc gacgacctcg ccgacctgtt cgacgtcagg
     2521 atcgacgagg aggaggtcga caccgtcggc ggcctgctcg ccaagctcgt cggccgggtg
     2581 ccgatccccg gctcgtcggc caggatcgcc ggcctgacac tgcgggccga gcggatggcc
     2641 ggtcgtcgcc accggctcgc gacagtcatc gtgtcggtcg gcgccgcggc ggcaggcaag
     2701 ggcatccccg ctgcagccga cgtggcgcgc gtgagccgca cggcggagtc ggcctcggcc
     2761 tcggccgact cgccgtccac
//
LOCUS       DATAIX010000532         3241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_280862, whole genome shotgun sequence.
ACCESSION   DATAIX010000532 DATAIX010000000
VERSION     DATAIX010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280862"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="VHM65_07950"
     CDS             <1..776
                     /locus_tag="VHM65_07950"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX2705676.1"
                     /translation="VGGPLLKVMLSESARRQGRTWLDVIEIQRHVLTYRHHVHDHSPD
                     LEEDGYRLLERVERHGLAGLPTSSSTAHISVVDDAGTACAITASSGYGSGATLPGTGL
                     MMNNCLGEPELNRIGLHALAPGTRLASNMAPTVGHGAGGARLAIGSPGADRITTALLQ
                     VLVRYCLMGVGLQEAIDRPRLHIRLLPDGSPQVEFEDDPDMDAAVTAAGLPALGHPAK
                     SMYFGGVGAAHLGADRALSAAADARREAATGVSPARATP"
     gene            complement(751..2817)
                     /locus_tag="VHM65_07955"
     CDS             complement(751..2817)
                     /locus_tag="VHM65_07955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HEX2705677.1"
                     /translation="MAMSSTTTGARPERDRPWVMRTYAGHSSAAASNALFRLNLAKGQ
                     TGLSVAFDLPTQTGYDPDHVLARGEVGKVGVPISHVGDMRGLFDQIPLRQMNTSMTIN
                     ATAMWLLALYEVVAEEQALAAGEDVAETVRSLAGTTQNDIIKEYLSRGTYIFGPAPSL
                     RLITDMVAYTVAKIPGWNPTNICSYHLQEAGATPVQEIAYSMSTAIAVLDAVRAGGQV
                     PPDKFADVVARISFFVNAGVRFVEEMCKMRAFVQLWDEVTRDRYGVTDAKARRFRYGV
                     QVNSLGLTEAQPENNVQRIVLEMLGVTLSKNARARALQLPAWNEALGLPRPWDQQWSL
                     RMQQVLAFESDLLEYPDIFDGSLVIEAKVSELLTGAREEVARIDAMGGAVAAVESGYM
                     KSALVASHATRRHRIESGDEVVVGLNRFTTTEENPLTVDLESAIQAVDPTVEAKAVAA
                     IRAWRAQRDADPQRAARAQAGLDRLRVDAEGSANLMAASLECARAGVTTGEWTQALRE
                     VFGEFRAPTGVSGTVGAGPVTSTGQGDAGMAEVRELVARTGRELGGRLRFLVGKPGLD
                     GHSNGAEQVAVRARDAGFEVIYQGIRLTPEQIVSAAVAEDVHCVGLSILSGSHMSLIP
                     DVLRGLEEAGAGDIPVIVGGIIPESDAAALRGMGVAAVFTPKDFGLTAIMGAVARTIR
                     ASRGLD"
     gene            complement(2859..>3241)
                     /locus_tag="VHM65_07960"
     CDS             complement(2859..>3241)
                     /locus_tag="VHM65_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX2705678.1"
                     /translation="SGAGIEAPCWASVDVIDTVEGDDVTVTGCLDVRTTPDVRARLHD
                     AIDCGTGPVHVHLAGAEIGDATGLGVLVGAHARARRAGRDFVLVDTSERTERLLRASR
                     LNRVLLRRMQRAGRSAPTVAPVTA"
BASE COUNT          464 a         1169 c         1146 g          462 t
ORIGIN      
        1 ccgtcggcgg tccgctgctc aaggtgatgc tcagcgagag cgcccgtcgg cagggccgca
       61 cctggctcga cgtcatcgag atccagcggc acgtgctgac ctaccgtcac cacgtccacg
      121 accactcccc cgacctcgaa gaggacggct accggttgct cgagcgcgtc gagcgccacg
      181 ggctcgccgg cctgccgacc tcgtcgtcga cggcgcacat ctcggtcgtc gacgatgcgg
      241 ggacggcctg cgcgattacc gcgtcgagcg gctacggctc cggggcaacg ctgcctggca
      301 ccgggctcat gatgaacaac tgcctcgggg agcccgagct caaccgcatc gggctgcacg
      361 cgctggcacc cggcacccgc cttgcctcga acatggcacc caccgtggga cacggcgcgg
      421 gcggtgcccg gctggccatc ggctctcccg gtgccgaccg catcacgacg gcgctacttc
      481 aggtgctcgt gcgctactgc ctcatggggg tcgggctgca ggaggccatc gaccgccccc
      541 ggctgcacat ccgcctgctg cccgacgggt cgcctcaggt cgagttcgag gacgaccccg
      601 acatggacgc agccgtcacc gcggccgggc tgcccgcgct cgggcacccc gccaagtcga
      661 tgtacttcgg gggcgtgggt gcggcacacc tcggtgccga ccgggcgctc agcgctgccg
      721 ctgacgcccg ccgcgaggcg gcgaccggcg tcagtccagc ccgcgcgacg ccctgatcgt
      781 ccgggcgacc gcgcccatga tcgcggtgag cccgaagtcc ttgggcgtga agaccgcggc
      841 aactcccatg ccgcgcaagg ccgccgcgtc cgactcgggg atgatccctc cgacgatgac
      901 cggtatgtcg cccgcccccg cctcctcgag gccgcgcagg acgtcgggga tgagcgacat
      961 gtgcgagccg gacaggatcg agagcccgac acagtggaca tcctcggcga cggcggcgga
     1021 gacgatctgc tcgggtgtga gccggatgcc ctggtagatc acctcgaacc ctgcgtcgcg
     1081 ggcccggacg gcgacctgct cggcgccgtt gctgtggccg tcgagaccgg gcttaccgac
     1141 gaggaagcgc agcctgccac cgagctcacg gcccgtcctc gccacgagct cgcggacctc
     1201 cgccataccg gcgtcaccct ggcccgtgct cgtgaccggc ccggcaccga ccgtcccgct
     1261 gacaccggtg ggcgcccgga actccccgaa cacctcacgc agcgcctggg tccactcgcc
     1321 ggtcgtgacc ccggcgcgag cgcattcgag cgaggcggcc atgaggttgg ccgagccttc
     1381 ggcgtccacc cggagccggt ccaggccagc ctgagcgcgg gccgcccgct gggggtcggc
     1441 atcgcgctgc gcgcgccacg cccggatcgc cgcgaccgcc ttggcctcga cggtggggtc
     1501 gacggcctgg atcgcggact cgaggtcgac cgtgagcggg ttctcctcgg tcgtcgtgaa
     1561 gcggttgagc ccgacgacca cctcgtcccc ggactcgatc cgatggcggc gagtcgcgtg
     1621 cgaggcgacc agggccgact tcatgtagcc gctctccacc gcggcaacgg cgccccccat
     1681 ggcgtcgatg cgcgccacct cctcgcgggc tcccgtcagc agctcgctca ccttggcctc
     1741 gatgaccagc gacccgtcga agatgtcggg gtactcgagc aggtcggact cgaacgccag
     1801 cacctgctgc atccgaagcg accactgctg gtcccatggg cgcggcagcc cgagtgcctc
     1861 gttccaggcc ggcagctgca gggcccgggc ccgtgcgttc ttggacagcg tgacgccgag
     1921 catctccagg acgatgcgct gcacgttgtt ctccggctgg gcctcggtca gccccaggga
     1981 gttgacctgc acgccatacc ggaagcggcg cgccttggcg tcggtgacgc cataccggtc
     2041 cctggtcacc tcgtcccaga gctggacgaa ggcgcgcatc ttgcacatct cctcgacgaa
     2101 gcgcaccccg gcattgacga agaaggagat ccgggccacg acgtcggcga acttgtccgg
     2161 cggcacctgg ccgccggcgc gcaccgcatc gagcaccgcg atggccgtcg acatcgagta
     2221 ggcgatctcc tggaccggtg tcgcgccggc ctcctggagg tggtagctgc agatgttggt
     2281 cgggttccag cccgggatct tggccacggt gtaggccacc atgtctgtga tgagccgcag
     2341 cgagggggcc ggaccgaaga tgtaggtccc ccgtgacagg tactccttga tgatgtcgtt
     2401 ctgggtcgtg ccggccaggg agcggaccgt ctcggcgacg tcctcgccgg ccgcgagcgc
     2461 ctgctcctcg gcgacgacct cgtagagcgc gaggagccac atcgcggtcg cgttgatcgt
     2521 catcgacgtg ttcatctggc gcagcgggat ctggtcgaac agcccccgca tgtcaccgac
     2581 gtgcgagatc gggacgccga ccttgccgac ctcgccccgg gccagcacgt ggtcggggtc
     2641 gtagccggtc tgcgtgggca ggtcgaaggc gaccgagagg ccggtctggc ccttggccag
     2701 gttgagccgg aacaaggcgt tgctcgcggc cgcactcgag tggccagcgt aggtgcgcat
     2761 cacccagggc cggtcgcgct cggggcgtgc gccagtggtg gtcgatgaca tggccatggg
     2821 gccgacggta cccgggccag tggtggggag ggagtgcgtc aggcggtgac aggcgccaca
     2881 gtgggggctg accgcccggc gcgctgcatc ctgcgtagca gcacccggtt gagccgggag
     2941 gcgcgcaaca ggcgctcggt gcgctcggag gtgtcgacga ggacgaagtc gcgccccgcc
     3001 cggcgagccc gcgcatgagc acccacgagg acaccgagcc cggtcgcgtc gccgatctcg
     3061 gcgccggcga ggtggacgtg gacgggcccg gtcccgcagt cgatggcgtc gtgcaggcgg
     3121 gcgcggacat cgggcgtcgt gcggacgtcg aggcacccgg tcacggtcac gtcgtccccc
     3181 tcgacggtat cgatgacatc gaccgacgcc cagcagggtg cctcgatgcc tgcaccggag
     3241 c
//
LOCUS       DATAIX010000533         1832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_118317, whole genome shotgun sequence.
ACCESSION   DATAIX010000533 DATAIX010000000
VERSION     DATAIX010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118317"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(349..1194)
                     /locus_tag="VHM65_07965"
     CDS             complement(349..1194)
                     /locus_tag="VHM65_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HEX2705679.1"
                     /translation="MTSFSDHELFSSDSHPDGPPEGERWSSWDGAMHGPTPRPDWVIT
                     ALGAVESDLGVLKTGKEADVHRVRRWLPCPDGGEPVRDTFMAAKRFRSHEHRMFHRDA
                     GYLEGRRVRRSREMRAMAKRTAFGRDMIGGQWAMAEFDALGRMWELGLPVPYPVQLSD
                     SEMLMEFIGSPEGVAAPRLAQSRPPADLLRDVHEQLRAAMLALAEQGWAHGDLSPYNV
                     LLDGERIVIIDWPQIVDVIGNPHGPEFLERDARNIARWFTSRGLPVDEGDFFGDLMAA
                     AVSRW"
     gene            1593..>1832
                     /locus_tag="VHM65_07970"
     CDS             1593..>1832
                     /locus_tag="VHM65_07970"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2705680.1"
                     /translation="MARLQQTDGLTEDQGELVKLVRQFVDEQIIPVATALEHKDEYPA
                     EIVAGMKEMGIFGLMIPEEYGGLGESLLTYALCVEE"
BASE COUNT          286 a          614 c          662 g          270 t
ORIGIN      
        1 ccccgcaacc aggttgacga cgctttctgg cacacctgcc cggccgtccc gtcccgccgg
       61 tcgacgaacg ggtccgcggt gccgcagtcg atgcggatcg ggatctgcga cagcgccgcg
      121 gaccgggtga agacatcgtg ggcgaggaag tcctcgagca ccatcgcccc ggtgtccgtc
      181 ccgtccctgc gggatcccgc tgaaccactg gcccgcgtca gcgccccagc cgtgcagcgc
      241 gacgacgacc cgcggtggtg agggtccgtc cagtgatcac cgggccaacg ctgcgacgat
      301 ccgggagtgg tgacggggtc ctgatcccgc tatcactccc cgttcgcctc accagcggga
      361 gaccgcggcg gccatgaggt cgccgaagaa gtcgccctcg tcgacgggca gcccgcgtga
      421 ggtgaaccag cgcgcgatgt tgcgggcgtc gcgctcgagg aactccggtc cgtgcgggtt
      481 gccgatcacg tcgacgatct ggggccagtc gatgatgacg atgcgctcac cgtcgagcag
      541 cacgttgtag ggcgagaggt ctccgtgcgc ccagccctgc tcggccaggg ccagcatcgc
      601 cgcgcgcagc tgctcgtgca cgtctcggag caggtcggcc ggcggccggc tctgggcgag
      661 ccggggcgcg gccacgccct cgggcgagcc gatgaactcc atgagcatct cggagtcgct
      721 cagctgcacg gggtagggca cgggcaggcc gagctcccac atgcgcccca gtgcgtcgaa
      781 ctcggccatg gcccactgcc cgccgatcat gtcgcggccg aaggcggtcc gcttggccat
      841 ggccctcatc tctcgggacc gccggacccg gcggccttcg agatagccgg cgtcgcggtg
      901 gaacatccgg tgctcgtggc tacggaaccg cttggccgcc atgaaggtgt ccctgaccgg
      961 ctccccgccg tcggggcagg gcagccagcg ccgcacgcgg tggacgtcgg cctccttacc
     1021 ggtcttgagc acgccgaggt cgctctcgac ggcgccgaga gcggtgatga cccagtcggg
     1081 ccggggggtg ggaccgtgca tggcaccgtc ccaggacgac cagcgctcgc cttcgggcgg
     1141 gccgtcgggg tgagagtcag aggagaagag ctcgtggtcg gagaatgagg tcaaggcagg
     1201 acttcctggt ggagggggtt gggttgcatg aggcacccag ggccgtcgtg tacatgtcgc
     1261 cctccttgga gtccgctgga acgtccgcat cgtatgacgc gcgctgaccc aagtgtcccg
     1321 gctccccagc gctcgtggca accggtttac ggcggcggca cctgccggca cctgccgaca
     1381 cctgccggca cctgccgaca cctgtcaccc ccgccgaggt cggggaatgt ccgcccaaag
     1441 acgtgggatg agcggacatt gcccgacttc gtcgaggggc aggaggccac cgggtggtgg
     1501 gactcacagc gaggtgggtg tcgctgggag ccgggcactc ggcatactcg cgagaggaac
     1561 cccaaccaac cgcaggcgta aggtgatccc ccatggcgcg acttcagcag accgatggac
     1621 tgaccgagga ccagggcgag ctggtcaagc tggtgcgcca gttcgtcgac gagcagatca
     1681 tcccggtcgc gaccgcgctc gagcacaagg acgagtaccc cgccgagatc gtggcgggca
     1741 tgaaggagat ggggatcttc gggctcatga tcccggagga gtatggcggg ctgggcgagt
     1801 cgctgctgac ctatgccctg tgcgtcgagg ag
//
LOCUS       DATAIX010000534         2981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_118334, whole genome shotgun sequence.
ACCESSION   DATAIX010000534 DATAIX010000000
VERSION     DATAIX010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118334"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VHM65_07975"
     CDS             complement(<1..529)
                     /locus_tag="VHM65_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705681.1"
                     /translation="MLRQASISVVLDLTALRGPELTAYLRDLPSVIESERAAWGLPHW
                     IVLDEAHSALGPGGAVEGLVRPTDRGYCLVTFRPDELAHDITAEVDVRIATGGPAEPT
                     KDRVATLIERGCAPRTFTIGARRTRHIRHWHKYTSAPLRPEQWFRFRRLDGSTVAAAV
                     NVAEFVHYLRVVDAPV"
     gene            complement(532..1479)
                     /locus_tag="VHM65_07980"
     CDS             complement(532..1479)
                     /locus_tag="VHM65_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX2705682.1"
                     /translation="MADVTGMIRAVAMDLDGTIATDDQVGDTVREAIGRARENQLATV
                     LVTGRILADLDTGFPGLRDLFDAIVAENGAVLDIDGFVHELGEALGPQLSAALSRAGI
                     PHRTGRILLGCPAEHANAVSDLVAALGLDAQLVRNRGQLMLVPAGISKGTGLLAALHE
                     LGLSPHNVLAVGDAENDLALLAAAEIGVAVANALPSVREHADLVLDRPNGCGVAALLG
                     GPLVAGRQPMQVSRRRSRSVPSADGSVATVPGAGANILVTGDTGSGKSHLAGLLIEQW
                     IDAGYSVLVVDVEGDYVGLGRLNKSSSSGASSHHTATSS"
     gene            complement(1608..>2981)
                     /locus_tag="VHM65_07985"
     CDS             complement(1608..>2981)
                     /locus_tag="VHM65_07985"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HEX2705683.1"
                     /translation="MTTREEREVAIREAGYNTFLLPSRVCYIDLLTDSGTSAMSDRQW
                     SAMMLGDEAYAGARSFDNLLRAVQETYGFPYVVPTHQGRGAEHLISRILIRPGQYVPG
                     NMYFTTTRAHQELAGGHFVDVVIDEAHDAQKWHPFKGNVDIAKLERLIADVGADNIAY
                     VNLAVTVNLAGGQPVSMANIRAVREVCDRHGIILWSDATRLAENAFFIQEREDGYADR
                     TCASIVLEMLSHFDGLTMSGKKDCLVNIGGFLAMRSEEILIKARELVVLFEGMPTYGG
                     LAGRDLEAMAVGMREAVDDHYLAHRVGQVRYLGEQLLEAGVPIVQPIGGHAVFLDAAS
                     FLPHLTQDELPAQALAAALFVESGVRSMERGIVSAGRNPDGTNRHPKLELVRLTIPRR
                     VYTDRHMDVVADAVIELFNRRESIEGLRFVYESPTLRFFTSRFEPVPVSVSRTDETRL
                     FELAAAH"
BASE COUNT          444 a          999 c         1089 g          449 t
ORIGIN      
        1 tcaccggggc gtccacgacc cgcagatagt gcacgaactc cgcgacgttg accgcagcgg
       61 ccaccgtgct cccgtcgagg cgccggaagc ggaaccactg ctcgggccga aggggcgccg
      121 aggtgtactt gtgccagtgc cggatgtgcc tggtgcggcg ggcgccgatc gtgaaggtcc
      181 ttggtgcaca acctcgttca atgagggtcg ccacgcggtc cttggtcggc tcggccgggc
      241 cgccggtggc gatgcgcacg tcgacctcgg cggtgatgtc gtgagccagc tcgtccggcc
      301 ggaacgtcac gaggcagtat cctcggtcgg tcggccggac cagcccttcg acggcacctc
      361 ccggtcccag tgccgagtgg gcttcgtcga gcacgatcca gtgcggcagg ccccacgcgg
      421 cccgctcgct ctcgatcacc gacggcagat cgcgcaggta tgccgtcagc tcgggtccgc
      481 gcagagccgt gaggtccagg acgaccgaga tcgaggcctg gcgcagcatg gtcacgagct
      541 cgtagctgtg tggtggctgc tggcccccga ggacgacgac ttgttgagcc ggccgagccc
      601 gacgtagtcg ccctcgacat cgacgacgag aacgctgtag ccggcgtcga tccactgctc
      661 gatgaggagt ccggcgaggt gggacttgcc cgagccggtg tctccggtga cgaggatgtt
      721 ggcacccgct ccggggacgg ttgcgaccga cccgtcggcg gagggtaccg atcgggacct
      781 tcgccgggac acctgcatcg gctgccggcc cgccacgagg ggtccaccca gcagggccgc
      841 caccccgcat ccgttcggcc ggtcgaggac aaggtcggcg tgctcacgca cggagggcag
      901 cgcgttggcc acggcgaccc cgatctcggc cgccgcgagc agtgccaggt cgttctcggc
      961 gtcgccgact gccaggacgt tgtgcgggga aagtccgagc tcgtgcagcg ccgcgagcag
     1021 gcccgtgccc ttggagatcc cggcggggac gagcatgagc tggccgcggt tgcggaccag
     1081 ctgggcgtcg aggccaagcg ccgccacgag atcgctcacc gcgttggcgt gctcggccgg
     1141 gcagccgagc aggattcgcc cggtccggtg cgggatcccc gcgcgggaga gcgcggcgct
     1201 gagctggggt ccgagggcct cgccgagctc atggacgaag ccgtcgatgt cgagcacggc
     1261 accgttctcg gcgacgatcg cgtcaaagag gtcgcgcagg ccaggaaacc cggtgtcgag
     1321 gtcggccagg atccgcccgg tgacgaggac ggtcgccagc tggttctcgc gggctcggcc
     1381 gatcgcctcc cgaacggtgt ccccgacctg gtcgtcggtc gcgatggtcc catcgaggtc
     1441 catcgcgacg gccctgatca tgccggtcac gtcagccacc tccgggatcc gtcgaggatg
     1501 ccctcttcct tgatcgcacc actccggggc cgtccgcaca agaccggaat cgacgcacgc
     1561 tccccgcggg cgccattgac cgcggggagc gtgcgtcgtg gcccgcctca gtgcgcggcg
     1621 gccagctcga acagccgcgt ctcgtcggtc ctggacacac tgaccgggac gggctcgaag
     1681 cgcgaggtga agaagcgcaa ggtcggcgac tcgtagacga agcgcaggcc ctcgatcgac
     1741 tcgcggcggt tgaacagctc gatgacggcg tcggccacga catccatgtg ccggtcggtg
     1801 tagacccgcc gcgggatcgt gagccggacg agctcgagct tggggtgacg gttcgtgccg
     1861 tcggggttgc gcccggccga gacgatgccg cgctccatgg accgcacgcc gctctcgacg
     1921 aacagcgccg ccgccagggc ctgcgcgggc agctcgtcct gggtcaggtg cggcaggaag
     1981 gacgccgcgt cgaggaagac cgcgtggccg ccgatgggct ggacgatcgg cacgccggcc
     2041 tcgagcagct gctcgccgag gtagcggacc tgcccgaccc gatgggcgag gtagtggtcg
     2101 tcgacggctt cgcgcatccc gacggccatc gcttcgaggt cgcggccggc gagcccgcca
     2161 tacgtcggca tgccttcgaa gaggacgacg agctcgcgcg ccttgatgag gatctcctcc
     2221 gagcgcatcg cgaggaagcc cccgatgttg accaggcagt ccttcttgcc cgacatcgtc
     2281 agcccgtcga agtgcgagag catctcgagc acgatggagg cgcaggtgcg gtcggcatac
     2341 ccgtcctcgc gctcctggat gaagaacgcg ttctcggcca ggcgggtcgc gtcggaccac
     2401 aagatgatgc cgtggcggtc gcagacctcg cggacggcgc ggatgttggc catcgagacc
     2461 ggctggcccc cggcgaggtt cacggtcacc gcgaggttga cgtaggcgat gttgtcggcg
     2521 ccgacgtcag cgatgagccg ctcgagcttg gcgatgtcga cgttgccctt gaacgggtgc
     2581 cacttctgcg cgtcgtgggc ctcgtcgatg acgacgtcga cgaagtgccc gccggcgagc
     2641 tcctggtggg cgcgggtggt cgtgaagtac atgttgcccg ggacgtactg gcccggccgg
     2701 atgaggatcc gcgagatgag gtgctcggcc ccgcggccct ggtgcgtcgg gacgacatac
     2761 gggaagccgt acgtctcctg gaccgcacgc aggaggttgt cgaaggaccg cgcgccggcg
     2821 taggcctcgt cgccgagcat catcgcgctc cactgccggt cgctcatcgc ggaggtgccc
     2881 gagtcggtga gcaggtcgat gtagcacacg cgacttggca gcaggaaggt gttgtagccc
     2941 gcctcgcgga tggcgacctc gcgctcctcc cgggtcgtca t
//
LOCUS       DATAIX010000535         1861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_58952, whole genome shotgun sequence.
ACCESSION   DATAIX010000535 DATAIX010000000
VERSION     DATAIX010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58952"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1145)
                     /locus_tag="VHM65_07990"
     CDS             complement(159..1145)
                     /locus_tag="VHM65_07990"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX2705684.1"
                     /translation="MELRWIDSGGTAHERAVGDLADLLTQPDGWVWLDVAEPDEASMA
                     SIQRTFGFHARAVHDCVERNHVPRLHYYHDYLFLVLHRPLAGVGGHVHYLELDQFIGA
                     NYLVTVHGPRNPAVALASLLEETRDAAERMRAGRLTPSTPYALSSAIVSTLIRRQEEL
                     VNHLANDVGLLEKRVMAQEDTDPRGFLEELFRVRHALLTVRTMCSQSREVYGRASTVM
                     TNAPTGVITSLVDILDQYDRVARISDSQLAFLQGVTDFYRARTDTKMTIAAERLAVIA
                     AITLPVTATSSVVGMNVIVNDSTHVGLLVVLLVLMAAMSLILLRWAKRQGWW"
     gene            complement(1163..>1861)
                     /locus_tag="VHM65_07995"
     CDS             complement(1163..>1861)
                     /locus_tag="VHM65_07995"
                     /inference="COORDINATES: protein motif:HMM:NF022674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
                     /protein_id="HEX2705685.1"
                     /translation="VEVARAALVDVAEPDTVGEHLHFAMDAERLATHYFACTARGYLG
                     WHWAVAVARAPRQKHATVCETSLLPGDDAVLAPTWVPYADRLAPGDLGAADVLPYRTD
                     DDNLVAGFEATGDEDVDATAFFELGLGRPRVLSAEGRECAAQRWYDGAHGPGAEAALH
                     ASAQCTSCGYYLPMAGALRAVFGVCANEWSPSDGRVVSLDHGCGAHSETDATPAVSAP
                     PAEHLVDELALDPM"
BASE COUNT          269 a          636 c          692 g          264 t
ORIGIN      
        1 cgtcataccg tcctggcatg ccggggagcg gtcgtggtgg ccgtgggcgg cttgcgccgg
       61 ggtggtgatc ggcctgctcg gctggggcta cctgcgccgg ggccggggca acgccgcggg
      121 gctgtaggtc accaccagcc aggtcaccac cagcgaggtc accaccagcc ctggcgtttg
      181 gcccagcgga gcaggatcag cgacatcgcc gccatgagga ccaggagcac gacgagcagg
      241 cccacgtggg tcgagtcgtt gacgatgacg ttcatcccga cgaccgagct cgtggccgtg
      301 accggcaggg tgatcgccgc gatcacggcg agccgctcgg ccgcgatcgt catcttggtg
      361 tcggtccggg cccggtagaa gtcggtcacg ccctgcagga acgcgagctg gctgtcactg
      421 atgcgcgcca cccggtcgta ctggtccaga atgtcgacga ggctcgtgat cacgccggtc
      481 ggggcgttgg tcatgaccgt gctggcccgc ccgtagacct cccggctctg cgagcacatc
      541 gtccgcaccg tcagcagcgc gtggcggacc cggaacagct cttcgaggaa gccccgcggg
      601 tcggtgtcct cctgggccat gacccgcttc tccagaaggc cgacgtcgtt ggcgaggtgg
      661 ttgacgagct cctcctggcg ccggatcaac gtcgagacga tggccgagga cagcgcgtat
      721 ggcgtcgacg gtgtcagccg cccagcgcgc attcgctcgg cggcgtcccg ggtctcctcg
      781 agcaggctgg cgagcgccac cgccgggttg cgcggaccgt ggaccgtcac gaggtagttg
      841 gctccgatga actggtccag ctcgaggtag tggacgtgtc caccgacccc ggcgagcggc
      901 cggtgcagca cgaggaagag gtagtcgtgg tagtagtgca gcctcgggac gtggttgcgc
      961 tcgacacagt catggacggc ccgggcgtgg aagccgaagg tccgctggat cgacgccatc
     1021 gacgcctcgt cgggttcggc gacatcgagc cagacccagc cgtccggttg ggtcagcagg
     1081 tcagccaggt cgccgaccgc gcgctcgtgc gcggtgccgc cggagtcgat ccaccgcagt
     1141 tccatcgcgc cctccctcgg tcctacatcg ggtcgagggc gagctcgtcc acgaggtgct
     1201 cggccggggg agccgacacg gcaggggtcg cgtcggtctc cgagtgagcc ccgcagccgt
     1261 ggtcgaggct gaccacccgg ccatcggacg gggaccactc gttggcgcag acaccgaaga
     1321 cggcccgcag cgccccggcc atcggcaggt agtagccgca tgaggtgcac tgcgccgacg
     1381 cgtgcagggc ggcctcggcc cccgggccgt gcgcgccgtc ataccagcgc tgggccgcgc
     1441 actcccggcc ctcggcggac agcacccgag gccggccgag acctagctcg aagaacgcgg
     1501 tggcatcgac gtcctcgtcg ccggtcgcct cgaagccggc cacgaggttg tcgtcgtcgg
     1561 tgcggtaggg caggacgtct gccgcgccga ggtccccggg ggcgaggcgg tcggcatacg
     1621 ggacccaggt gggggccagg accgcgtcgt cacccgggag caggctcgtc tcgcacacgg
     1681 tggcgtgctt ctgccgcggg gcgcgcgcga ccgcgacggc ccagtgccag cccagatagc
     1741 cgcgagccgt gcacgcgaag tagtgggtgg cgaggcgctc ggcatccatg gcgaaatgca
     1801 ggtgctcgcc caccgtgtcc ggctcggcga cgtcgacgag ggccgcccgg gcgacctcga
     1861 c
//
LOCUS       DATAIX010000536         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_221223, whole genome shotgun sequence.
ACCESSION   DATAIX010000536 DATAIX010000000
VERSION     DATAIX010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221223"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..>2315
                     /locus_tag="VHM65_08000"
     CDS             42..>2315
                     /locus_tag="VHM65_08000"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEX2705686.1"
                     /translation="MARRSAPPPGEDVAERIIDIDVEEEMKGAFLEYAYSVIYSRALP
                     DARDGLKPVQRRILYAMSELGLRPDRGHVKSSRVVGEVMGKYHPHGDTAIYDALVRMA
                     QPFTMRLPLVDGHGNFGSLDDGPAASRYTEARLAPAALLMTSGLDEETVDYVPNYDDQ
                     LLQPEVLPAAYPNLLVNGASGIAVGMATNMAPHNLVEVVGAARYLIEHPSCSLDDLMR
                     FVPGPDLPTGGRIIGLDGIRDAYLSGRGSFRTRATTRIESVTARRKGIVVTELPYLVG
                     PEKVIEKIKDLVQAKKLLGISDVKDLTDRTHGLHLVIEVKNGFHPEAVLDQLFRLTPM
                     EDSFGINNVALVDGQPRTLGLKDLLRVYVDFRTSVVRRRSEYRLRKREERLHLVDGLL
                     VAILDIDEVIQLIRAADDAATAKARLMGVFDLSDAQATYILDLQLRRLTRFSLLELET
                     ERAELERQIEALRAILSDAALLLRTVSSELAEVAKAHGTPRRTVLLESAGVPATVTSP
                     LEVSDDPCWVLLSSTGLLARTSSADPVPTHGRRARHDAIVAAVRTSARGSFGLVTSRG
                     RMIRLSALELPTLVATNDAPSLSGGAPLAAYVDLPRGEEPLTLASLDADAAGLALGAA
                     HGVVKRVLADYPPNRDELELISLKDGDVVVGAAMVPSDDWDLVFLTSDAQLLHFPASS
                     VRPQGRPAGGMAGVKLASGARATFFGALDPARDAVVVTSAGSSAALPGTEPGSLKVTP
                     YAEYPAKGRATAGVRCHR"
BASE COUNT          338 a          857 c          795 g          325 t
ORIGIN      
        1 acgatggacc gcgagaccca gccaaggaga gccgaccgcg tatggcccga agaagcgccc
       61 ccccacccgg cgaggacgtc gccgagcgga tcatcgacat cgacgtcgaa gaggagatga
      121 agggggcgtt cctcgagtac gcctactcgg tcatctactc gcgcgccctg ccggacgccc
      181 gtgacgggct caagccggtc cagcggcgca tcctctacgc gatgagcgag ctcgggctgc
      241 ggcccgaccg cggccacgtc aagtccagcc gcgtcgtcgg cgaggtcatg ggcaagtacc
      301 acccgcacgg cgacacggcg atctacgacg cgctggtgcg catggcgcag ccgttcacga
      361 tgcggctgcc gctcgtcgac gggcacggca acttcggctc cctcgacgac ggcccggccg
      421 cgtcgcgcta caccgaggcc cggctcgccc ccgcggcgct gctcatgacg agcgggctcg
      481 acgaggagac cgtcgactac gtccccaact acgacgacca gctcctccag cccgaggtgc
      541 tgcccgcggc atacccgaac ctgctcgtca acggagccag cggcatcgcc gtcggcatgg
      601 cgaccaacat ggcgccgcac aacctggtcg aggtcgtcgg cgcggcgcgc tacctcatcg
      661 agcacccgag ctgctcgctc gatgacctca tgcggttcgt gcccggtccg gacctgccga
      721 ccgggggacg catcatcggc ctcgacggca tccgcgacgc ctatctgagc gggcggggct
      781 cgttccggac ccgcgccacg acccggatcg agagcgtcac cgcgcggcgc aagggcatcg
      841 tcgtcaccga gctgccctac ctcgtcggcc ccgagaaggt catcgagaag atcaaggacc
      901 tcgtccaggc caagaagctg ctcggcatct ccgacgtcaa ggacctcacc gaccgcaccc
      961 acggcctgca cctcgtcatc gaggtcaaga acggcttcca ccccgaggcc gtcctcgacc
     1021 agctcttcag gctgaccccg atggaggact cgttcggcat caacaacgtc gccctcgtcg
     1081 acggccagcc tcgcacgctc gggctcaagg acctcctgcg ggtgtatgtc gacttccgca
     1141 cctcggtggt ccggcggcgc tcggagtacc ggctgcgcaa gcgtgaggag cggctgcacc
     1201 tcgtcgacgg gctgctcgtg gcgatcctcg acatcgacga ggtgatccag ctgatccggg
     1261 cggccgatga cgcggccacc gccaaggccc ggcttatggg cgtgttcgac ctctccgatg
     1321 cccaggcgac ctacatcctc gacctgcagc tgcgccggct cacgaggttc tcgctgctcg
     1381 agctggagac cgagagggcc gagctggagc gccagatcga ggcgctgcgg gcgatcctgt
     1441 ccgacgcggc gctgctgctg cgcaccgtgt cgagcgagct ggccgaggtc gccaaggccc
     1501 atgggacccc gcgccggacg gtgctgctgg agtcggcagg ggtgccggcg accgtgacga
     1561 gcccccttga ggtcagcgac gacccctgct gggtgctgct ctcctcgacc gggctgctgg
     1621 cgcggacgtc gtcggctgac ccggtgccca cgcacgggcg ccgggcccgg cacgacgcga
     1681 tcgtcgccgc ggtgcgcacc tcggcccggg ggtcgttcgg tctcgtgacg agccgggggc
     1741 ggatgatccg gctctcggcg ctggagctgc cgacgctcgt ggcgaccaac gacgcgccgt
     1801 cactgtccgg cggggcgccg ctggcggcat acgtcgacct gccccgcggg gaggagccgc
     1861 tcaccctggc ctcgctcgat gctgacgccg cgggcctggc gctcggcgcg gcgcacgggg
     1921 tggtcaaacg ggtgctcgcc gactaccccc cgaaccggga cgagctcgag ctgatctcgc
     1981 tcaaggatgg tgacgtcgtc gtcggggccg cgatggtgcc ctcggacgac tgggacctgg
     2041 tcttcctcac ctccgatgcc cagctgctgc acttcccggc ctcgtcggtg cggccgcagg
     2101 gacgcccggc cggggggatg gctggcgtca agctcgccag cggggcacgg gcgacgttct
     2161 tcggggcact cgacccggcg cgggacgccg tcgtcgtcac gtcagcggga tcctccgccg
     2221 ccttgcccgg caccgagccc ggctcgctca aggtcacccc gtatgccgag taccccgcca
     2281 aggggcgcgc caccgctggg gtccggtgtc accgg
//
LOCUS       DATAIX010000537         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_221263, whole genome shotgun sequence.
ACCESSION   DATAIX010000537 DATAIX010000000
VERSION     DATAIX010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221263"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..1209)
                     /locus_tag="VHM65_08005"
     CDS             complement(244..1209)
                     /locus_tag="VHM65_08005"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX2705687.1"
                     /translation="MARGGLVRASRTRLLGSVRASVLASVLASVLVAGSLAACSAEDA
                     GVTSATTSTSAAPGATTADPTPRATTTPVTPAPGATASVSAATLDAVVSTALGSRRST
                     STVCLVDLRTGATYVYNPSLRIHTASIVKVLVLVSVIRYQADRGKGLTSTQRALASRM
                     IRWSDNNATSALVRQIGHGSVTKVLTALGMSSTTFGGSSWGHTLTSARDQARLVTALV
                     TGTPVLTAAQRGYVLDLMEDVTPSQHWGVGTEPAGATAQVKNGWYPDSGGWRVNSIGH
                     VTGRGGRNYVLTVLTRGSPSMGSGIARVSAVAKAVYTAMAAPLAK"
     gene            1371..>1666
                     /locus_tag="VHM65_08010"
     CDS             1371..>1666
                     /locus_tag="VHM65_08010"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF014749.4"
                     /note="catalyzes the formation of ornithine and
                     carbamylphosphate from citrulline in the arginine
                     catabolic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase subunit F"
                     /protein_id="HEX2705688.1"
                     /translation="MAAVSLTGRSLLRETDLSLAEWEYLLDLAAELKAATRARREPRH
                     LEGRTIALIFERTSTRTRCDFEVAAHDQGAFTTYLEPWVSQIGHKDPSSDGDSIA"
BASE COUNT          241 a          589 c          606 g          230 t
ORIGIN      
        1 gaaccgcgga gtcaccatcg cgttggccga tcggggtggg cctgcctagt gtctgggtca
       61 tgaccagcgc cgacgccgga tcgccgaccg gggtgcctga cctatgcggc cgaccagatc
      121 cgggtcgagc aggacgccac catgaggtca tacctgagcc ggtggcgcgc gctgctcgag
      181 ggcccgcccg acgtcagcgt atggcggccc tcaacgccgc ggtgacgtcg cgggacgccc
      241 gggctacttc gccaggggag ccgccatcgc ggtgtagacg gccttggcga cggcgctgac
      301 ccgcgcgatc ccggacccca tcgaggggct gccccgggtg aggaccgtca ggacgtagtt
      361 gcgcccgccc cgtccggtga cgtgcccgat cgagttgacc cgccagccgc cggagtcggg
      421 gtaccacccg ttcttgacct gggccgttgc gcccgcgggc tcggtcccga cgccccagtg
      481 ctgcgagggc gtcacgtcct ccatgaggtc gaggacgtag ccgcgctggg ctgcggtgag
      541 gaccggcgtg cccgtgacga gggccgtgac cagccgggcc tggtcacgcg ccgaggtcag
      601 ggtgtgcccc cacgaggaac cgccgaaggt cgtcgaggac atgccgaggg ccgtgaggac
      661 cttggtcacc gacccatggc cgatctggcg cacgagcgcg ctcgtcgcgt tgttgtcgct
      721 ccacctgatc atccgggagg ccagcgccct ctgggtcgag gtgaggcctt tgccccggtc
      781 ggcctggtat cggatgaccg agacgagcac gaggaccttg acgatgctcg ccgtgtggat
      841 ccgcagactg gggttgtaga cataggtcgc cccggtgcgc aggtcgacga ggcagacggt
      901 cgaggtcgac cgccgcgaac ccagtgccgt gctgacgacg gcgtcgagcg tagccgcgct
      961 caccgaggcc gtcgcgccgg gggccggggt caccggtgtg gtcgtggccc ggggcgtggg
     1021 gtcggcggtc gtcgcccccg gcgcggctga ggtcgacgtc gtggccgagg tgacgcccgc
     1081 gtcctcggca gaacaggcgg ccagcgagcc cgcgaccagc accgatgcga gcaccgaagc
     1141 gagcaccgat gcgcgcaccg acccgagcag ccgtgtccgg ctcgctcgca cgaggccccc
     1201 tcgcgccatc cccacctcca ggtccggtgt gccaatctac cccgaggcac gacgtcgtcg
     1261 tgcgggctgc gcaaccaccg gtgcccactg ggcggaactg gcccgcctcc tggctcaggg
     1321 gggtcggcac aatgtccgct tcggcatccg cgacctgcga aggagagacc atggccgccg
     1381 tctcgctcac gggccgaagc ctgctgcgtg agaccgacct gtccctggcc gagtgggagt
     1441 acctcctcga cctggccgcc gagctcaagg cggccacgag ggcccggcgc gagccgaggc
     1501 acctcgaggg ccgcactatc gcgctcatct tcgagaggac ctcgacccgc acccgctgcg
     1561 acttcgaggt ggccgcgcac gaccagggcg cgttcacgac atacctcgag ccgtgggttt
     1621 cgcagatcgg gcacaaggac ccgagttcgg acggggacag catcgc
//
LOCUS       DATAIX010000538         3364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_191361, whole genome shotgun sequence.
ACCESSION   DATAIX010000538 DATAIX010000000
VERSION     DATAIX010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191361"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..2403
                     /locus_tag="VHM65_08015"
     CDS             202..2403
                     /locus_tag="VHM65_08015"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX2705689.1"
                     /translation="MLVVRLVLGLLMTLVCLGIAGRRLVFLYRLGKAGQPVPAGRRPG
                     AGETVKAEFVEVAGQRRLLAWTGPGLAHFAVFWGFMVLVLTIIEGFGSLVWPTFHIPV
                     VGNHAWLGFFEDLFALLCMGAVLAFVAIRVKEAPKDKGRASRFFGSHLGPAWFVLFMI
                     FNVVWTLIFARAAQINAQEVNTATDGRLGFLKGAFAAQWIAGLFEPLGRHTNEILETV
                     LLLLALGVLLGFTVFVTYSKHLHIFLSIPNVAFARRPRALGALLPLYSGGTKVDFEDP
                     GDDDLLGVGTIDGFTWKGLLDFGTCTECGRCQSQCPAWNTDKPLSPKLLIMTLRDHAL
                     AKAPYLLAGSDEARAAQPDGVRAEAGRPLVGPAGDEAAVVDEHGVSGEGVVPFDMLWS
                     CTTCGACVEQCPVDIEHVDHIVDMRRYQVLMESAFPEEAGVMLRNLEHAGDPWGRGAR
                     MRLEWAAPLDFELRVFGADGEDKIPEDVDYLYWVGCAGALDDAAMRTARAVATLLHEA
                     GVEFMVLGEAEACTGDPARRMGHEFLFQMLATANVETLNEAGAKRIVVTCAHCFNTLS
                     NEYPEVGGHYEVVHHTTLLAKLVAEGKLTPIERIDAAVTYHDPCYLGRHNRIFAPPRE
                     VLGAIPGARVTEMPRSRERSFCCGAGGARMWMDENLGTRINLNRSDEALATKATLITA
                     ACPFCITMLGDGVAQRQLEGTAPEQVEVTDVAEILLRSVRPDLTDAAASLG"
     gene            2471..>3364
                     /locus_tag="VHM65_08020"
     CDS             2471..>3364
                     /locus_tag="VHM65_08020"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2705690.1"
                     /translation="MTDTTTASIGHLGGAGLDLGSLNLMVEALDDFVAQALSPDLTLE
                     LDHEDRCPEDIVRAMNDPQQLGVQLVFIPEAYGGMDGGAFDSYRVCEVMARHDIGLAT
                     AVFATYLGSDPILVGATHEQKERWLGAIADQGVLFAYGATEPEAGSDLSALTTIATPT
                     TNDDGEVTAYTINGRKQWISNGSIADYTTILAATPAGPSWFVVERNTPGFTSGPPEDK
                     HGLRLSNTAALYLDDVQVPADALIGLVPGKGMVQAQQVFGYTRLMVAAFGLGGGWEAL
                     DRAIQYSINRVQGGGPLCEKQG"
BASE COUNT          498 a         1224 c         1116 g          526 t
ORIGIN      
        1 atccagcacc gtgcgggcat gcagagcagc aagacgatcg tcgcggtcaa caaggacccc
       61 aaggcgccga tcttcggcat ctcggacttc ggtgtcgtcg gagacctgca caaggtcgtc
      121 cccgcactca tcgacgagat cggcaagcgc aagccctgat gtgacctgag ccgagcccgg
      181 gggggagaag gagtccgcta gatgttggtc gttcggctcg tcctgggcct gctcatgacg
      241 ctcgtctgcc tcgggatcgc cggacggcgg ctcgtcttcc tctaccgcct cggcaaagcc
      301 gggcagcccg ttcccgcggg ccgcaggccg ggggccggcg agacggtcaa ggccgagttc
      361 gtcgaggtgg ccgggcagcg caggctgctg gcgtggaccg ggcccgggct ggcccacttt
      421 gcggtcttct ggggcttcat ggtcctcgtc ctgacgatca tcgagggctt cgggtcgctt
      481 gtctggccga cgttccacat cccggtcgtc ggcaaccacg cgtggctcgg cttcttcgag
      541 gacctcttcg cgctgctctg catgggcgcg gtgctcgcgt tcgtcgcgat ccgggtcaag
      601 gaagccccga aggacaaggg ccgggcctca cgcttcttcg ggtcgcacct cggcccggcg
      661 tggttcgtgc tcttcatgat cttcaacgtc gtgtggaccc tgatcttcgc ccgcgccgcc
      721 cagatcaacg cccaggaggt caacaccgcc acggacgggc ggctcgggtt cctcaaggga
      781 gccttcgccg cgcagtggat cgccgggctg ttcgagccgc tgggtcgtca taccaacgag
      841 attctcgaga ccgtcctcct cctgctcgcg ctgggtgtcc tgctcgggtt cacggtcttc
      901 gtgacctaca gcaagcacct gcacatcttc ttgtcgatcc ccaacgtcgc gttcgcgcgg
      961 cggccgcggg cgctcggcgc gctgctgccc ctctactccg gcgggacgaa ggtcgacttc
     1021 gaggacccgg gcgacgacga cctcctcggt gtcggcacga tcgacggctt cacctggaag
     1081 gggctgctcg acttcggcac gtgcaccgag tgtgggcgct gccagagcca gtgccccgcg
     1141 tggaacaccg acaagccgct gagccccaag ctcctcatca tgaccctgcg cgaccacgca
     1201 ctggccaagg cgccctacct gctcgcgggc tccgacgagg ccagggcggc ccagcccgac
     1261 ggggtcaggg ccgaggccgg gcggcccctc gtgggcccgg ccggtgacga ggccgcggtc
     1321 gtcgacgagc acggcgtgtc cggcgagggc gtcgtgccct tcgacatgct gtggtcctgc
     1381 accacgtgcg gcgcctgcgt cgagcagtgc cccgtcgaca tcgagcatgt cgaccacatc
     1441 gtcgacatgc gccgctacca ggtgctcatg gagtcggcct tccccgagga ggccggcgtc
     1501 atgctgcgca acctcgagca cgccggtgac ccgtggggcc gcggggcgcg gatgcgcctc
     1561 gagtgggccg cgccgctcga cttcgagctg cgtgtcttcg gcgccgacgg cgaggacaag
     1621 atccccgagg acgtcgacta tctctactgg gtcggctgcg caggtgcgct cgacgacgcc
     1681 gcgatgcgga ccgcccgggc ggtcgcgacc ctgctgcacg aggccggcgt cgagttcatg
     1741 gtgctcggcg aggccgaggc ctgcaccggc gacccggccc ggcggatggg gcacgagttc
     1801 ctcttccaga tgctcgcgac ggcgaacgtc gagaccctca acgaggccgg cgccaagcgg
     1861 atcgtcgtca cctgcgccca ctgcttcaac accctgtcca acgagtaccc cgaggtcggc
     1921 ggccactacg aggtcgtcca ccacaccacg ctgctcgcca agctcgtcgc cgagggcaag
     1981 ctcaccccca tcgagcgcat cgacgccgcg gtgacctacc acgacccgtg ctacctcggt
     2041 cggcacaacc ggatcttcgc gccgccgcgc gaggtgctcg gcgccatacc cggggctcgg
     2101 gtcaccgaga tgccgcgctc gcgcgagcgg tcgttctgct gcggggcggg cggggcccgg
     2161 atgtggatgg acgagaacct cggcacccgg atcaacctca accgctccga cgaggccctg
     2221 gcgacaaagg ccactctcat cacggcggcc tgcccgttct gtatcacgat gctcggcgac
     2281 ggtgtcgccc agcgccagct cgaaggcacc gcgcccgagc aggtcgaggt caccgacgtg
     2341 gccgagatcc tgctccgctc ggtccggccc gacctcaccg acgccgcagc atcgctcggc
     2401 taggcgcaca cttcaggtac cacgctttgt acccgctcat ccgtccgcac ctcgacccaa
     2461 gggcttcgat atgactgaca ccaccaccgc ttccatcggc catctcggcg gggctggcct
     2521 cgacctgggc agcctcaacc tcatggtcga ggcgcttgac gacttcgtcg cccaggccct
     2581 ctcgccggac ctgacccttg agctcgacca cgaggaccgc tgcccggagg acatcgtccg
     2641 ggccatgaac gacccccagc agctcggcgt gcagctcgtc ttcatccccg aggcgtatgg
     2701 cgggatggac ggcggcgcgt tcgactccta ccgggtgtgc gaggtgatgg cgcggcacga
     2761 catcggcctg gcaacggcgg tcttcgcgac ctacctcggg tccgacccga tcctcgtcgg
     2821 cgcgacgcac gagcagaagg agcgctggct cggcgccatt gccgaccagg gcgtcctgtt
     2881 cgcctatggc gcgaccgagc ccgaggcggg ctcggacctc agcgcgctga cgaccatcgc
     2941 taccccgacc accaacgacg acggggaggt gacggcatac acgatcaacg gtcgcaagca
     3001 gtggatctcc aacgggtcca tcgccgacta cacgacgatc ctcgccgcca cccccgccgg
     3061 tccgtcctgg ttcgtcgtcg agcggaacac ccccggcttc acctcgggtc cgccggagga
     3121 caagcacggc ctgcgcctgt ccaacaccgc cgcgctctac ctcgacgacg tccaggtgcc
     3181 cgccgacgcg ctcatcggcc tggtcccggg caagggcatg gtccaggccc agcaggtctt
     3241 cggctacacc cggctcatgg tcgcggcgtt cgggctcggt ggcggctggg aggcgctcga
     3301 ccgggcgatc cagtactcca tcaaccgggt gcagggcggc ggcccgctgt gcgagaagca
     3361 gggc
//
LOCUS       DATAIX010000539         3652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_131717, whole genome shotgun sequence.
ACCESSION   DATAIX010000539 DATAIX010000000
VERSION     DATAIX010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131717"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1328)
                     /gene="tyrS"
                     /locus_tag="VHM65_08025"
     CDS             complement(60..1328)
                     /gene="tyrS"
                     /locus_tag="VHM65_08025"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX2705691.1"
                     /translation="MSDIFEELQWRGSVAQTTGEDALREAFAQGPVTAYCGFDPTAPS
                     LHFGNFVQLVLLRRLQRAGHRVICLVGGSTGLIGDPKPTAERVLRTKEQTAEWVERIK
                     SLVEPFLDFGGDNPAVMVDNLDWTAPMSALDFLRDVGKHFRVNQMIRKEAVSARLESQ
                     EGISYTEFSYQLLQALDFLHLYREYGCTLQTGGSDQWGNLTAGVDLIHRTEGASVHVL
                     TTPLLTDAAGTKYGKSEGNAIWLSADMTSPYAFYQYFVNVEDASVVGLLKMFTDLGRE
                     EVDDYARQVEQEPFRRAAQRHLAASVTTLVHGAEATVSVQAASGALFGTGDLGALDAG
                     TLRDATAELPGGEVTPGMSLTDALVAVGLAESRNAARRTISDGGASLNNVRITDAGAV
                     LGEPDFLHGSVLILKRGRRTLAAGRRRGAP"
     gene            1498..2256
                     /locus_tag="VHM65_08030"
     CDS             1498..2256
                     /locus_tag="VHM65_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX2705692.1"
                     /translation="MSSVRSVAVLSDIHGVLPVLEAVLDEPDVQAADLIVVTGDHASG
                     PMPVATLDRLVGLGERGVLVRGNADRELVQIRAGEDNPHPESVWAAGQLRQDQVGLLA
                     EMPHPVTIDVAGFGPVLFCHGTPGSDTDVVLVDSRLDRWAEAFAGLPAQIRTVVCGHT
                     HMPFVRLVDRRLVLNPGSVGMPYGRHGGAWALLEDGQVSLRHTAVDIDRACARVVAES
                     SYPDRVTWADEYVRSANSDTDALHAFGPLDGRQG"
     gene            complement(2253..2921)
                     /locus_tag="VHM65_08035"
     CDS             complement(2253..2921)
                     /locus_tag="VHM65_08035"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX2705693.1"
                     /translation="MTRRLPRSFFARDVLEVAPALLGARLTHAGVTVRVTETEAYAGH
                     LADPGSHAYRGETPRTAPMFGPAGFTYVYFTYGMHWMLCLVTGPAGLAQAVLVRAGEV
                     VAGHDLAAARRPGVRPRDWCRGPARLAASLALTGQHTGLDFCRPSPRAPTGATTGPGP
                     AGRVALVARGSAQAVERSLIRSGPRVGVSGPGGDGTSYPWRFWIDGEATVSVFRPGVI
                     RRRG"
     gene            complement(2931..>3652)
                     /gene="argH"
                     /locus_tag="VHM65_08040"
     CDS             complement(2931..>3652)
                     /gene="argH"
                     /locus_tag="VHM65_08040"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX2705694.1"
                     /translation="ALSDRDFLLDLAYACAVAMMHLSRLCEELILWSSDEFGFVTMDD
                     GYSTGSSIMPQKKNPDVAELARGKAGRLIGDLAGLLTTLKGLPLAYNRDLQEDKEPVF
                     DAVDTLEVLLPAFAGMLATLTFHTERLESLAPQGFALATDIAEWLVRARVPFRDAHEV
                     AGACVRVCEQRGIELWDLSDDDLAAISPVLTPGVRDVLSVAGSLASRNATGGTAPVRV
                     AEQLAAAGARARLARSFTEPG"
BASE COUNT          515 a         1316 c         1293 g          528 t
ORIGIN      
        1 aaacgccaaa tcgggcgcgg aacaaggcaa tgccgtggct ggcgtgcgct cacgcgcgct
       61 cacggggcac cccggcggcg cccggcggcg agggtccggc gccctcgttt gaggatgagg
      121 accgacccgt gcaggaagtc gggctcaccc agcactgccc cggcatcggt gatccggacg
      181 ttgttgagcg aggcccctcc gtcggagatc gtgcgccggg cggcgttgcg gctctcggcc
      241 agcccgacag ccaccagggc atcggtgagc gacataccgg gtgtgacctc gcctccgggc
      301 agctcggccg tcgcgtcgcg cagtgtccct gcgtcgagcg cgccgaggtc ccctgtgccg
      361 aacagggccc cggatgccgc ctggaccgat accgtcgcct ccgcgccatg caccagtgtc
      421 gtcacggatg ccgccagatg ccgttgggct gcgcggcgga agggctcctg ctcgacctgg
      481 cgcgcgtagt cgtcgacctc ctcgcgcccg aggtcggtga acatcttgag caggccgacc
      541 accgaggcgt cctcgacgtt cacgaagtac tggtagaagg cgtaggggct cgtcatgtcc
      601 gccgacagcc agatcgcgtt gccctcggac ttgccgtact tcgtccccgc ggcgtcggtc
      661 agcaggggcg tcgtcagcac gtggaccgac gcaccctcgg tccggtggat caggtcgaca
      721 cccgcggtga ggttgcccca ctggtcgctg ccccccgtct gcagggtgca gccgtactcg
      781 cggtagaggt gcaggaagtc cagcgcctgc agcagctggt agctgaactc ggtgtaggag
      841 atgccctcct ggctctccag ccgcgcggag accgcctcct tgcggatcat ctggttgacc
      901 cggaagtgct tgccgacgtc gcgcaggaag tccagggccg acatcggcgc cgtccagtcc
      961 aggttgtcga ccatgactgc ggggttgtcg cccccgaagt cgagaaacgg ctcgacgagg
     1021 gacttgatcc gctcgaccca ctcggccgtc tgctccttgg tccggagcac ccgctccgcc
     1081 gtcggcttcg ggtcgccgat cagcccggtc gaccccccga cgaggcagat gacccggtgc
     1141 ccggcacgct ggagccgccg cagcagcacg agctggacga agttgccgaa gtgcagagac
     1201 ggtgccgtcg ggtcgaaccc gcagtaggcc gtgactggtc cctgcgcgaa cgcctcgcgg
     1261 agcgcgtcct caccggtggt ctgcgccacc gacccgcgcc actgcagctc ctcgaagatg
     1321 tcactcacgc tcgccggtcc ttcctcgcct ggcacccggc gtggggtgcc tcatccctgg
     1381 ccatgagcgc caagcctgcc tcacgtcctg cgccggcagc gaacgggtat cgaccctcgg
     1441 tattccccgc gcctatcgtc acggggtatg tcgtcacggg gtatgtcgtc acggggtatg
     1501 tcgtctgtcc gctcggtcgc tgtgctgtcc gacatccatg gcgtgctgcc cgtcctcgag
     1561 gccgtcctcg acgagcccga cgtgcaggcc gccgacctca tcgtcgtcac gggcgaccac
     1621 gcctccgggc ctatgccggt ggcgaccctc gaccggctcg tcggtctggg cgagcgcggg
     1681 gtgctcgtgc gcggcaacgc cgaccgggag ctggtccaga tccgtgccgg cgaggacaac
     1741 ccccatcccg agtcggtgtg ggcggcgggc cagctgcgcc aggaccaggt cggcctgctc
     1801 gcggagatgc cgcatccggt caccatcgac gtggccggtt tcggtccggt cctgttctgc
     1861 cacggcaccc ccgggagcga caccgatgtc gtgcttgtgg acagccgcct cgaccgctgg
     1921 gccgaggcat tcgccgggct gccggctcag atccgcaccg tggtctgcgg ccacacgcac
     1981 atgcccttcg tccggctcgt cgaccgtcga ctggttctca accccggaag cgtcgggatg
     2041 ccctatggcc gccacggtgg cgcatgggcg ctgctcgaag acgggcaggt cagcctgcgc
     2101 cacaccgccg tcgacatcga ccgggcctgt gcgcgggtcg tggcggagtc gtcctacccc
     2161 gatcgggtga cctgggccga tgagtatgtc cgcagtgcca acagcgacac ggacgcactg
     2221 catgccttcg gtcccctgga cggccgccag ggctagcccc ggcggcggat cacgccgggg
     2281 cggaagaccg agaccgtcgc ctcgccgtcg atccagaacc tccaggggta tgacgtgccg
     2341 tcacctccgg gcccgctcac tccgacgcgg ggtccgctgc gaatgaggga ccgttcgacg
     2401 gcctgcgccg acccccgcgc cacgagcgcg acgcgccccg ctgggcccgg gccggtcgtc
     2461 gccccagtcg gcgccctcgg cgatggccgg cagaagtcca gtcccgtgtg ctgcccggtc
     2521 agggccagcg acgcggcgag ccgggccggg ccacggcacc agtcgcgcgg ccggaccccg
     2581 gggcgtcggg ccgcggcgag atcgtgaccg gccacgacct ccccggcccg cacgaggacg
     2641 gcctgcgcga gcccggccgg ccccgtcacg agacacagca tccagtgcat gccgtaggtg
     2701 aagtagacgt aggtgaaccc ggccgggccg aacatcgggg ccgtgcgtgg tgtctcaccc
     2761 cggtaggcgt gcgatcccgg gtcggcgagg tgcccggcat acgcctcggt ctcggtgacc
     2821 cgcacggtga cgccggcatg ggtcagcctc gccccgagca gcgccggggc gacctcgagc
     2881 acgtcgcggg cgaagaagct ccgcggcagg cgccgggtca cagcgcgagg ctacccgggc
     2941 tcggtgaagg accgggccag ccgggcgcgg gcccctgcgg ccgcgagctg ctcggcgacg
     3001 cgcaccgggg cggtcccgcc ggtggcgttg cgcgaggcga gcgagccggc gacgctgagg
     3061 acgtcgcgaa cccctggggt cagcacgggg ctgatcgcgg ccaggtcgtc gtcgctgagg
     3121 tcccacagct cgatgcctcg ctgctcgcag acgcgcacgc aggcgcccgc gacctcgtgg
     3181 gcgtcgcgga acggcacgcg cgcgcggacg agccactcgg caatgtccgt cgccagcgcg
     3241 aaaccctggg gtgccaggga ctcgagacgc tcggtgtgga aggtcagggt cgccagcatc
     3301 ccggcgaagg ccggcagcag cacctcgagc gtgtccacgg cgtcgaagac cggctccttg
     3361 tcctcctgca ggtcccggtt gtaggccagc ggcagcccct tgagcgtggt gaggagcccg
     3421 gcaaggtcac cgatgagccg gccggccttg ccgcgagcga gctcggccac gtccgggttc
     3481 ttcttctgcg gcatgatcga ggagccggtc gagtagccgt cgtccatcgt gacgaaaccg
     3541 aactcgtcgc tggaccacag gatgagctcc tcgcacagcc gcgagaggtg catcatcgcg
     3601 accgcgcagg cgtaggccag gtccagcagg aagtcgcggt cggacaacgc gt
//
LOCUS       DATAIX010000540         3491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_251016, whole genome shotgun sequence.
ACCESSION   DATAIX010000540 DATAIX010000000
VERSION     DATAIX010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251016"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1066)
                     /locus_tag="VHM65_08045"
     CDS             complement(92..1066)
                     /locus_tag="VHM65_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2705695.1"
                     /translation="MDPVTGDRGPGDASPAGRSVVVENLTRTFGSVTALSQLNVEMPQ
                     GVVGLVGANGAGKSTLIKILLGLLPPTSGRARVLGMDCATQGLAIRQIVGYMPESECL
                     PPDVSATEFVVHLARMSGLPSAVARERTADTLRHVGLYEERYRPIGGYSTGMKQRVKL
                     AQSLVHDPKLVLLDEPTNGLDPAGRDEMLTLIQQISTDFSISVVVTSHLLGELERISD
                     HLVVIESGVLQRSTSTADATQLSGVVLVEVTDGGPELVARLRAAGAQVADDGGAHTFT
                     VELAGEWVLDAVQDGAADLGVGLVRMQRRRHHLTEIFRPEQEGAHVRR"
     gene            1616..3193
                     /gene="glpK"
                     /locus_tag="VHM65_08050"
     CDS             1616..3193
                     /gene="glpK"
                     /locus_tag="VHM65_08050"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX2705696.1"
                     /translation="MADFVGAVDQGTTSTRFMIFDHDGREAGRHQLEHEQILPRAGWV
                     EHNPQEIWDRTQTVIASALAGAGLAAADLDSIGITNQRETTIVWDRRTGRPYANAIVW
                     QDTRTDRIASRLERDGHGETIRRKAGLPPATYFSGGKLAWLLGNIDGLRQDAEAGHAV
                     FGTPDTWVLWNLTGGPDGGVHVTDVTNASRTMLMDLQSLAWDDELLALFGVPRQMLPE
                     IRPSSDPRLYGTTTSHGPLRAEVRLSGALGDQQAATVGQVCFAPGEAKNTYGTGNFML
                     LNTGEHLVRSRHGLLTTVAYQLGDARPAYALEGSIAVTGSAVQWLRDQLGIISGASEI
                     EGLASQVQDSGGMYFVPAFSGLFAPYWRSDARGAIVGLSRFNTNAHLARATLEAICYQ
                     TKDVADAMAADTEAAGFPVDFSVLKVDGGVTANELCMQIQADILGVEVSRPKCAETTA
                     LGAAYAAGLATGFWSGTDELRANWAEDRRWTPQWTDAQRAAGHAGWLKAVARTLDWVP
                     AEGTDAGTGAEPAKDTA"
     gene            3190..>3491
                     /locus_tag="VHM65_08055"
     CDS             3190..>3491
                     /locus_tag="VHM65_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2705697.1"
                     /translation="MRSLAPFPLNPTTRQAAIESMAGAAELDVLVVGGGVTGTGCALD
                     AVTRGLSTGLVEQRDFASGTSSRSSKLIHGGLRYLEMFDFRLVAEALKERGLLLTRL"
BASE COUNT          537 a         1300 c         1177 g          477 t
ORIGIN      
        1 gtagtgccgg aagccgatgt cgtggatgac gtcgtcagcc ggcgcagcga gcagcgcggg
       61 cacggctgat gcagcggtac cggggacgtc ctcaacgccg gacatgtgcg ccctcctgct
      121 ccggccggaa gatctcggtg aggtggtggc gccgtcgctg catccggacg agtccgacgc
      181 cgaggtcggc cgcgccgtcc tgcaccgcgt cgagcaccca ttcgccggcg agctccacgg
      241 tgaaggtgtg tgcgccgccg tcgtcggcca cctgtgcccc ggcggcgcgc agccgcgcga
      301 cgagctccgg tccgccgtcg gtgacctcca ccagcacgac cccgctgagc tgggttgcgt
      361 ccgccgtgga ggtggaccgc tgcagcacgc cgctctcgat caccacgagg tggtcggaga
      421 tccgctcgag ctcgccgagc aggtgcgagg tcaccacgac cgagatggag aagtccgtgc
      481 tgatctgctg gatcagggtg agcatctcgt cgcgaccggc cggatccagc ccgttggtgg
      541 gttcatcgag cagtacgagc ttcgggtcgt gcacgagcga ctgggcgagc ttcacgcgct
      601 gcttcatgcc ggtggagtac ccgccaatcg gtcggtaccg ctcctcgtac aggccgacgt
      661 ggcgcaacgt gtcggcggtg cgttcgcgcg cgaccgccga cggcagcccg gacattcgtg
      721 ccaggtgcac cacgaactcg gtggcagaca cgtccggggg caggcactcg ctctccggca
      781 tgtatccgac gatctggcgg atcgccaggc cctgcgtcgc acagtccatg ccgagcaccc
      841 gcgcccgacc cgacgtcggt ggcagcaggc cgagcaggat cttgatcaac gtcgacttac
      901 ccgcaccatt ggcgcccacc aggccgacga cgccctgggg catctcgaca ttgagctggg
      961 acaacgccgt gaccgagccg aaggtacggg tcaggttctc gaccacgacg gatcgcccgg
     1021 ccggcgacgc gtcaccaggg ccgcggtcgc cagtcacggg gtccatacct gtgatcatgc
     1081 ctgcccgcgg gccccccgcg ccatcaggga ctccccctga gacaaccctg aggggggcgc
     1141 actcctccgc gcgggcgccc ttcgtgccca gacgcgaggt ctcctcacgt cgcccgaggt
     1201 tcccggtcgt gccgatcccg aacttgctcg cgaccgaccg cactatctcc cactctgatc
     1261 gcagccggga cacacctccg gtcatgtcac tgcgggtgag acaggggcgg caccactcgc
     1321 ggacgccgta tcgactcagc gccaacgagt gccgcccgga cgccgccgag aagcgctgca
     1381 acctaccgag aacaccccgc gctgcgcggc gacgagcgcc gtcatacctg tgcccacgct
     1441 gtgagcgcgg acccggccgg caccgagcgc cagcgccagc gccaggtgcg gtgctgtgga
     1501 ggacgggacc cccgtgaagg ggtgtggacc aggtccggcg cgggttcgag cgaggtcacc
     1561 gcgatgcgcg tcaccggccc cgcccgggca aggtgtgctg tgacaggctg gggctatggc
     1621 tgatttcgtc ggagcagtcg accaagggac gacgagcacc cggttcatga tcttcgacca
     1681 cgacggccgc gaggccggcc gccaccagct cgagcacgag cagatcctgc ccagggcggg
     1741 ctgggtcgag cacaatcccc aggagatctg ggaccgcacc cagacggtca tcgcctcggc
     1801 cctcgcaggg gcggggctgg ccgctgccga cctggactcg atcggcatca ccaaccagcg
     1861 cgagacgacc atcgtctggg accggcgcac cggacgcccc tacgccaacg ccatcgtctg
     1921 gcaggacacc cgcaccgacc gcatcgccag ccggctcgag cgcgacggcc acggcgagac
     1981 gatccggcgc aaggccggcc tgccaccggc gacctacttc tccggtggca agctcgcgtg
     2041 gctgctcggg aacatcgacg gcctccgcca ggacgccgag gccgggcatg ccgtcttcgg
     2101 caccccagac acgtgggtcc tgtggaacct caccggcggc cctgacggcg gcgtccacgt
     2161 caccgacgtc acgaacgcca gccgcacgat gctcatggac ctgcagtccc ttgcctggga
     2221 cgacgagctg ctcgcgctgt tcggcgtgcc ccgccagatg ctcccggaga tccgcccgtc
     2281 gagcgacccc cgcctctatg gcacgacgac ctcgcatggc cctctccggg ccgaggtcag
     2341 gctcagcggc gccctcggtg accagcaggc cgccaccgtc ggccaggtct gtttcgcccc
     2401 cggcgaggcc aagaacacct atggcacggg caacttcatg ctgctcaaca ccggtgagca
     2461 cctcgtccgc agccggcacg ggctgctcac caccgtggcc taccagcttg gtgacgcgcg
     2521 cccggcatac gcgctggaag gctcgatcgc cgtcaccggc tcagccgtcc agtggctgcg
     2581 cgaccagctc ggcatcatca gcggcgccag cgagatcgag gggctcgcga gccaggtgca
     2641 ggactccggc ggcatgtact tcgtgccggc cttctcgggt ctgttcgccc cgtactggcg
     2701 ctcggacgcc cgcggcgcga tcgtcggtct gagccgtttc aacaccaacg cccacctggc
     2761 ccgggcgacc ctcgaggcga tctgctacca gaccaaggac gtcgccgacg cgatggccgc
     2821 cgacaccgag gcggccggct tcccggtcga cttcagcgtc ctcaaggtcg acggcggcgt
     2881 caccgccaac gagctgtgca tgcagatcca ggcggacatc ctcggcgtcg aggtgagccg
     2941 cccgaagtgc gccgagacga ccgcgctcgg ggccgcgtat gccgccggcc tcgccaccgg
     3001 gttctggtcc ggcaccgacg agctgcgcgc caactgggcc gaggaccgac gctggacccc
     3061 gcagtggacc gacgcccagc gcgccgcggg gcacgcgggc tggctcaagg ccgtcgcccg
     3121 cacgctcgac tgggtgcccg ccgagggcac cgacgcaggc accggcgcag aaccagccaa
     3181 ggacaccgca tgagatcgct cgcgcccttc ccgctcaacc cgacgacccg ccaggcggcc
     3241 atcgagtcca tggccggcgc cgcggagctc gacgtcctcg tcgtcggcgg tggcgtgacc
     3301 ggcaccggat gcgccctcga cgcggtgacc cggggtctgt cgaccggcct ggtcgagcag
     3361 cgtgacttcg cctccggcac gtcgagccgc tcgagcaagc tgatccacgg cggcctgcgc
     3421 tacctggaga tgttcgactt ccgtctcgtc gccgaggccc tcaaggagcg gggcctgctg
     3481 ctgacccgcc t
//
LOCUS       DATAIX010000541         2299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_191436, whole genome shotgun sequence.
ACCESSION   DATAIX010000541 DATAIX010000000
VERSION     DATAIX010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191436"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VHM65_08060"
     CDS             complement(<1..678)
                     /locus_tag="VHM65_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705698.1"
                     /translation="MSQTTASHPSAPPPAIPGRGGVTPAPGHAPATGTAGASGGRVQV
                     REAVRGLLSGTPGRMRLLGAVAALSAVLFGAAAALNLSANDAAIERAAGTTAQVVRVQ
                     GMYADLLLADATATNSFLVGGLEKPEQRVVYEQAMARAAAGIAGAAQAEPADGAALAA
                     LNASVQSYAQLVEQARAYNRQGVPVGAQYLKLASAGLRSDALPVLDAITTADTSRSGD
                     EFGRAGSV"
     gene            complement(675..>2299)
                     /locus_tag="VHM65_08065"
     CDS             complement(675..>2299)
                     /locus_tag="VHM65_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2705699.1"
                     /translation="PSPVTGPADGLRQSLDALAAVAARAGADPRASRLEGLTFAAAIA
                     ESAPRAAADWAGAAGLAPAVPAPGAGTAAPGPGFADERQNRAFFDAASRGRKWRQAPT
                     TLLSLLVAQGSPAAADYARALTEVASAACLIGEVSMRVTGNASVAAAAQLAAVPARFP
                     DVYRPTGTQLPVPDGIPGDDALGTTADQLPGIPQGVQGQAAPASPNAPNGQPAPPAKT
                     VEELLAELDALIGLARVKREVHRQVALLTVEQKREKAGLKSATMTRHLVFVGNPGTGK
                     TTVARMVGGIYRALGLLSQGQLVEVDRSELVAGYLGQTAIKTAEVCAKSTGGVLFIDE
                     AYALGGDQYGAESVNTLVKEMEDRRDDLVVIVAGYPDPMVAFIGQNPGLASRFTTTIE
                     FEDYSDDELVAILHSLAAAADYDVTPAAQVRFREILAATPRTRAFGNGRFARNLLEQA
                     IGRHAWRLREAADPSTQELRDLLPHDFEERADSDPPPAQDTQDTQEPAEQGPELDTTD
                     HSSDQMHDSPGEGGAPGPPGPLPPESEQEENR"
BASE COUNT          269 a          820 c          877 g          333 t
ORIGIN      
        1 gaccgagccg gctcggccga actcgtcccc ggaccgcgag gtgtcggccg tggtgatcgc
       61 atcgaggaca ggcagcgcgt cggagcgcag tccggcgctg gcgagcttga ggtactgcgc
      121 gccgacggga accccctggc ggttgtaggc ccgggcctgc tcgacgagct gggcgtagga
      181 ctggaccgag gcgttgagtg cggccagtgc ggcgccgtcg gcgggctcgg cctgggcggc
      241 cccggcgatc ccggccgcgg cccgcgccat ggcctgctcg tagacgaccc gctgctcggg
      301 cttctccagg ccaccgacga ggaaggagtt cgtcgcggtc gcgtcggcga gcaggaggtc
      361 ggcatacatc ccctggacgc ggaccacctg cgcggtcgtg ccggccgccc gctcgatcgc
      421 ggcgtcgttg gccgacaggt tgagcgctgc ggcggctccg aagagcacgg ccgacagcgc
      481 cgcgacggcc ccgagcaggc gcatcctccc gggggtgccg gacagcaggc ctcggaccgc
      541 ctcgcggacc tggacgcgtc cgcccgacgc acctgccgtg ccggttgccg gggcatggcc
      601 cggcgcaggc gtgacacccc cacggcctgg tatggcgggt ggcggcgccg atggatggct
      661 ggccgtcgtc tggctcaccg gttctcctcc tgctcggact cgggcggtag gggacccggg
      721 ggacccgggg cgccgccctc gccgggcgag tcgtgcatct ggtcagacga atggtcggtg
      781 gtgtcgagtt ccggtccctg ctcggccggc tcctgcgtgt cctgcgtgtc ctgcgcgggg
      841 ggcgggtcgg agtcggcgcg ctcctcgaag tcgtgcggca gcaggtcgcg cagctcctgg
      901 gtgctcgggt cggctgcctc acggagccgc caggcgtgcc ggccgatcgc ctgctcgagc
      961 aggttgcgag cgaaccggcc gttgccgaag gccctcgtcc gcggtgtcgc ggcgaggatc
     1021 tcccggaacc ggacctgcgc ggcgggcgtc acgtcgtagt cggcggccgc agcgaggctg
     1081 tgcaggatcg cgacgagctc gtcgtcggag tagtcctcga actcgatcgt cgttgtgaag
     1141 cggctggcca gtcccgggtt ctggccgatg aaggcgacca tggggtcggg gtaccccgcg
     1201 acgatgacga cgaggtcgtc gcggcggtcc tccatctcct tgacgagggt gttgaccgac
     1261 tccgcgccat actggtcgcc gcccagagcg taggcctcgt cgatgaagag caccccgccc
     1321 gtcgacttcg cacacacctc ggcggtcttg atcgcggtct gcccgaggta gccggcgacg
     1381 agctcggagc ggtcgacctc gacgagctgg ccctggctga gcaggccgag cgcccggtag
     1441 atccccccga ccatccgggc cacggtcgtc ttgccggtcc cggggttgcc gacgaagacg
     1501 aggtgccggg tcatcgtcgc gctcttgagc ccggccttct cgcgcttctg ctcgacggtg
     1561 agcagcgcga cctgccggtg gacctcacgc ttgacgcgcg ccaggccgat cagcgcgtcg
     1621 agctcggcca gcagctcctc gacggtcttg gccggcggcg ccggctgccc gttgggtgcg
     1681 ttgggagaag ctggtgccgc ctggccctgg accccctggg gtatgccggg cagctgatct
     1741 gccgtcgtgc ccaatgcgtc gtcacccggg atcccgtccg gcactggcag ctgcgtgcca
     1801 gtcggccggt agacatctgg gaatcgcgcc gggactgcgg ccagctgcgc cgcagcggcg
     1861 accgacgcgt tgcctgtgac ccgcatgctg acctcaccga tcaggcaggc cgccgacgcg
     1921 acctcggtca gggcgcgcgc gtagtcggcg gcggcggggg agccctgggc gacgagcagg
     1981 ctcagcagtg tggtcggcgc ctggcgccac ttgcgccccc gcgacgccgc gtcgaagaag
     2041 gcccggttct gtcgttcatc ggcgaagccc ggaccgggcg ccgcggtgcc cgctccgggc
     2101 gcgggtacgg ccggagccag gccggctgcc ccggcccagt ctgctgcggc cctcggggcc
     2161 gactcggcga tggccgcagc gaaggtgagc ccttcgagcc gcgacgccct cgggtcggcg
     2221 ccggccctag ccgccaccgc ggcgagcgcg tccagggact ggcgcagccc gtccgccggc
     2281 ccggtgaccg gactcggcc
//
LOCUS       DATAIX010000542         1687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_89279, whole genome shotgun sequence.
ACCESSION   DATAIX010000542 DATAIX010000000
VERSION     DATAIX010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89279"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="VHM65_08070"
     CDS             complement(<1..994)
                     /locus_tag="VHM65_08070"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2705700.1"
                     /translation="MTRMVVVGNGMVGARFVEDLLAADRTGRYRVTVVGDDECEPYNR
                     VLLCEVVAGLHDLGSLTLPTPKDPRLVLRRGDPAVALDREDRAVVTASGARHRYDQVV
                     LATGSTARIPPLGGLGDNPGRLPRGVHPLRTIDDARDIVAATLNARHAVVLGAGVLGL
                     EAACGLARRGIAVTVVHPAAALMERQLDEHASRVVEQAMRGLGIDHRVGVGAQDALLH
                     HGALRGVLLADGETIDADLLVLTTGTIASTRLATGAGLRADRGVLVGPGLATLDDARV
                     FAIGDCAQPPEGGTGLVAQGWEQSRRLAAVLTSAENGPAGSVLTSRAPASRVSSD"
     gene            complement(991..>1687)
                     /locus_tag="VHM65_08075"
     CDS             complement(991..>1687)
                     /locus_tag="VHM65_08075"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX2705701.1"
                     /translation="HLASRLGAASTWSTDPAEVFDELARASAGGRADYSGLSHVRLED
                     EAMPLYWPCPRTPDGGPPHPGTPRLFLDRFPTADGRARLVPVEHDGPADDVRADAPVY
                     LVTGRVLQHYQSGAQTRRVPELDRLAPAAYVELHPLLAQRLDVSEGDLVRLSSARGAV
                     EARVRLTPDIRTDTVFMPFHWGGAGSANRVTSDATDPISGMPAFKVCAVEISRVPLPV
                     AVPLPDDAVEVFV"
BASE COUNT          226 a          594 c          658 g          209 t
ORIGIN      
        1 ggtccgatga cacccgtgac gccggagccc gtgacgtcag cacggacccg gccgggccgt
       61 tttcggcgct ggtgagcacc gcagcgagcc gccgggactg ctcccagccc tgggcgacca
      121 gaccggtgcc accctcgggc ggctgggcac agtccccgat ggcgaagacg cgggcgtcgt
      181 ccagggtggc caggccgggc ccgacgagga cgccgcgatc ggccctgagc ccggcgccgg
      241 tggccagccg ggtactggcg atcgtgccgg tagtcagcac cagcaggtcg gcatcgatcg
      301 tctcgccgtc ggccagcagc acgccccgca gcgcgccatg gtgcagcagg gcgtcctgag
      361 cgccgacgcc cacgcgatgg tcgatgccga gaccgcgcat cgcctgctcg accacccggc
      421 tggcgtgctc gtcgagctgg cgttccatga gggcggcggc cgggtggacg accgtgacgg
      481 cgatcccgcg gcgggccagc ccgcaggcgg cctccagccc gagcaccccg gcaccgagca
      541 cgacggcgtg ccgggcgttg agcgtggcgg cgacgatgtc gcgggcgtcg tcgatggtgc
      601 gcagcgggtg cacgcctcgg gggaggcggc cagggttgtc gcccaagccg cccagcgggg
      661 ggatgcgggc cgtgctgccg gtggccagca ccacctggtc gtaacggtga cgagctcccg
      721 atgcggtgac gactgcgcgg tcctcgcggt cgagtgcgac ggcggggtcg ccgcggcgca
      781 ggaccagcct cgggtccttc ggcgtcggga gcgtgaggga gccgaggtcg tgcaggcccg
      841 cgacgacctc acacagcagc acccggttgt agggctcgca ctcgtcgtcc cccaccacgg
      901 tgacgcggta gcgcccggtc cggtcggccg cgaggaggtc ctcgacgaac cgggcgccga
      961 ccatcccgtt gccaacgacg accatccgcg tcatacgaac acctccacag cgtcgtctgg
     1021 caacgggacc gcgactggca gcggaacccg gctgatctcg acggcgcaca ccttgaacgc
     1081 gggcattccc gagatggggt ccgtggcgtc gctggtcacc cggttcgcgc tgccggcgcc
     1141 gccccagtgg aacggcatga acacggtgtc ggtcctgatg tcgggcgtca gacgcacccg
     1201 ggcctcgacg gcgccgcgcg cgctcgagag ccggacgagg tccccctccg acacgtcgag
     1261 gcgctgggcc agcaaggggt gcagctcgac atatgccgcc ggggcgaggc ggtcgagctc
     1321 cggcacccgc cgagtctggg cgccggactg gtagtgctga aggacccggc cggtgacgag
     1381 gtagaccggc gcgtcggcgc ggacgtcgtc ggcgggtccg tcgtgctcga ctgggaccag
     1441 acgggcccgg ccgtcagccg tggggaagcg gtcgaggaac agccgtgggg tgcccgggtg
     1501 tggtggcccc ccgtcagggg tgcgcgggca gggccagtac agcggcatgg cctcgtcctc
     1561 gagtcgaacg tggctgaggc cgctgtagtc ggcgcggccg ccggcgctgg cgcgggccag
     1621 ctcgtcgaag acctccgcgg ggtcggtgct ccacgtgctg gccgcgccga gccgggatgc
     1681 caggtgc
//
LOCUS       DATAIX010000543         2450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_131809, whole genome shotgun sequence.
ACCESSION   DATAIX010000543 DATAIX010000000
VERSION     DATAIX010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131809"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1974
                     /locus_tag="VHM65_08080"
     CDS             <1..1974
                     /locus_tag="VHM65_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX2705702.1"
                     /translation="SASASASASASGSGSGSGSGSTEPAGPASGILPTPASHRVFRVS
                     RRPALDYELDPVRDRVLLRLLSSITPGLDVLRGYRRRWLRPDVFAGVAVAAYLVPQVM
                     AYTAIVNVPPVTGLWTALAALIVYAILGGSRVLSPGPESTVALMAGAAIAPLVAGSPD
                     RAIALTAALSLIVAGWCLIARIFRLGVVADLLSQPLLVGYLAGGAVLMIVGQLGKATR
                     TSVNGISITDQIRSFSAAVGGTHLPTLMVCAGTLALLLFVRWVRPSWPAPLIAVAVAT
                     VVCTVAGLAEHGVRIVGAVPTGLPAPHLPAVSWSDVQSLLIAGLGVAVVAYGDNTLIG
                     RGFPSPVDEDQDDNVIDPQQELVALAGVHAAVGLVGGFPVSSSGSRTALAIAAGARTQ
                     VYSLVSAACVVIVLFVAGPLISNLPQASLAAVVFYAASKLVAWRDVVRLARFRTNELL
                     LAVAAMLGTVVLGILLGVGVAVALSLLEMAQRLARPHEGVLGRVPGLPGMHDVDDHPN
                     AQTLPGLMVYRYDAPLFFANVSDLQRRALLAVHQENAAFPDRPVRWFILNVEANVEVD
                     ITAADGLRQLHNDLADRGARLGLARVKHDLLVPLARAGLTELIGSDMLFPTLPVAEAA
                     YLDWVAANGLEVVVPDRAGNPDAAPPAASSAGG"
     gene            complement(2035..>2450)
                     /locus_tag="VHM65_08085"
     CDS             complement(2035..>2450)
                     /locus_tag="VHM65_08085"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEX2705703.1"
                     /translation="RVKKLVGSQYEIDAAMRVSDLSELIDFPFPEDEDYVTLAGLVYK
                     ELGSVPEVGESVMLDGGRLSVLEMDHHRITLVKFEDTAVGDDGSIRLTDSSSAADTLP
                     SVETRHRAQAARVPGPTGAPGSEAPDVASRIRRRA"
BASE COUNT          275 a          929 c          845 g          401 t
ORIGIN      
        1 tccgcgtccg cgtccgcgtc cgcgtccgcg tccgggtccg ggtccgggtc cgggtctggg
       61 tccacggagc ctgccggccc tgcgagcggg atcctcccga cgccggcctc gcaccgggtg
      121 ttccgggtca gccgccgacc cgcgctggac tacgagctgg accccgtccg ggaccgagtc
      181 ctgctgcggc tcctgtcctc catcactccc gggctcgatg tcctgcgcgg ctaccggcgc
      241 cggtggctgc gaccggacgt cttcgcgggc gtcgcggtcg cggcatacct cgtcccgcag
      301 gtcatggcgt acaccgccat cgtcaacgtc ccgccggtga ccgggctgtg gaccgcgctc
      361 gccgcgttga tcgtctacgc gatcctcggc ggctcccggg tgctctcgcc gggccccgag
      421 tcgaccgttg cgctcatggc cggagccgcc atcgccccgc tcgtcgcggg cagccccgac
      481 cgggcgatcg cactcaccgc tgccctgagc ctcatcgtcg ccgggtggtg cctgatcgcc
      541 cggatcttcc ggctcggtgt cgtggccgac ctgctgtccc agccgctgct cgtcggctac
      601 ctggccggcg gcgcagtcct gatgatcgtg ggccagctcg gcaaggcgac tcgtacgtcc
      661 gtgaacggca tcagcatcac cgaccagatc aggtcgttca gcgccgcggt gggcgggacg
      721 cacctgccca cgctcatggt gtgcgcgggc accctggcgc tgctcctgtt cgtgcgctgg
      781 gtgcgtcctt cgtggcccgc gccgctcatt gcggtggccg tggccacggt cgtgtgcacg
      841 gtcgccgggc ttgccgagca cggggtccgg atcgtcggag ccgtgccgac cggtctgccc
      901 gcgccccacc tgccggccgt gtcctggagc gacgtccagt cactgctgat cgccggcctg
      961 ggcgtggccg tggtcgccta cggggacaac accctcatcg gccgcggctt cccgagtccg
     1021 gtcgacgagg accaggacga caacgtcatc gacccgcaac aggagctcgt ggcgctcgcg
     1081 ggcgtccacg cggcggtggg tctcgtgggc ggcttcccgg tgtcgtcctc cggctcccgc
     1141 acggccctgg ccatcgccgc gggggcgcgg acgcaggtct actccctggt cagcgcggcc
     1201 tgcgtcgtca tcgtgctgtt cgtcgcaggc ccgctcatct cgaaccttcc gcaggcctca
     1261 ctcgccgcgg tggtgttcta cgccgcgagc aagctcgtgg cgtggcgcga cgtcgtccgg
     1321 ctcgcccggt tccggaccaa cgagctgctc ctggcggtgg ccgccatgct cggaacggtc
     1381 gtcctcggca tcctgctcgg ggtgggtgtc gccgtcgccc tcagcctgct cgagatggct
     1441 cagcgccttg cccgcccgca cgagggagtc ctgggtcgcg tgccgggact gccgggcatg
     1501 cacgacgtcg acgaccatcc gaacgcccag accctgcccg ggctcatggt ctaccggtat
     1561 gacgcgcccc tcttcttcgc caacgtcagc gatctgcagc gccgcgccct gctcgccgtg
     1621 caccaggaga acgctgcctt ccccgaccgc ccggtgcgct ggttcatcct caacgtcgag
     1681 gccaacgtcg aggtcgacat caccgcggcc gacggtctgc gccagctcca caacgacctc
     1741 gccgaccggg gtgcgcggct cggtctggcc cgggtcaagc acgacctgct cgtcccgttg
     1801 gcccgggccg ggctcaccga gctcatcggc tcggacatgc tcttccccac gctgcccgtg
     1861 gccgaggcag cctatctcga ctgggtggcg gccaacggcc tcgaggtcgt ggtgccggac
     1921 cgggcgggga acccggacgc ggcgcctccc gccgcctcct ccgcgggagg gtgaggagcg
     1981 gtccccgctt gcgcgcctcg cgactggtcg acgaccgtgg cgacgcaggg ggcctcaggc
     2041 ccgtcggcga atacgggacg cgacgtcggg tgcttcggag cccggcgccc ccgtggggcc
     2101 cggcacccgg gcagcctgcg cgcggtggcg tgtctcgacc gaggggaggg tgtccgcggc
     2161 cgacgagctg tcagtcaggc ggatgctgcc gtcgtccccc acggccgtgt cttcgaactt
     2221 gaccagggtg atccggtggt ggtccatctc gagcaccgac aaccggccgc catcgagcat
     2281 caccgactcg ccgacctcgg gcacgctgcc gagctccttg tagaccaggc cggccagggt
     2341 gacgtagtcc tcgtcctccg ggaacgggaa gtcgatcagc tcgctcagat cggagacccg
     2401 catcgcggcg tcgatctcgt actggctgcc gacgagcttc ttgacccgcc
//
LOCUS       DATAIX010000544         4088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_59330, whole genome shotgun sequence.
ACCESSION   DATAIX010000544 DATAIX010000000
VERSION     DATAIX010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59330"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..1171)
                     /locus_tag="VHM65_08090"
     CDS             complement(224..1171)
                     /locus_tag="VHM65_08090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HEX2705704.1"
                     /translation="MKILIVGAGATGGYFGALLTRAGRDVTFLVHPHRAEVLRTRGLR
                     IVATGDAWAVQPQLVTADQLTETFDLVVLSVKATALRSAIDDLAPAVGPQTMIVPFLN
                     GIGHLDALQARFGPSTVMGGVVKVATQLNEEGDIVQVAPGASLLFGALDGPDTSGAQA
                     AHAALDGAGFDVGISDNIMAGMWHKWVFIATLGALTCLMRSSVGDIAAEPGGRDLATG
                     ILAEAAAVASAAGYQLPPVELGTTATMITETGSPLTSSMFRDLATQRHTEVEHILGDL
                     TFRARNLAVPTPLLDSAVLHLRVYERRLAAWERQQADRP"
     gene            1451..1975
                     /gene="ssb"
                     /locus_tag="VHM65_08095"
     CDS             1451..1975
                     /gene="ssb"
                     /locus_tag="VHM65_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX2705705.1"
                     /translation="MNESFVTVCGNVVAKPEVRTAKTGTQFATFRVASTSRRKSQDGS
                     AFVDGPTNFVNVVAFRGLGANVTASLERGHPVIVYGRLRVKQWTKDDRSGTSVEIDAY
                     NVGHDLNRGTTAYSRQMRSLGDTNDRLSDEAVQEATQELEAAFIVEDGMAVDPRTGEV
                     ADQQTRPGYFEEAS"
     gene            2057..3739
                     /gene="ettA"
                     /locus_tag="VHM65_08100"
     CDS             2057..3739
                     /gene="ettA"
                     /locus_tag="VHM65_08100"
                     /inference="COORDINATES: protein motif:HMM:NF008775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX2705706.1"
                     /translation="MAEFIYTMSRARKAVGDKLILDDVTMSFYPTAKIGVVGPNGAGK
                     STILKIMAGLDQPSNGEARLSPGYTVGILLQEPSLNEVKTVLGNVEEGVGEIKAKLDR
                     YNEIAMEMAEPDADYDKLGDEMSALQDDIDHANAWDLDSRLEQAMDALRCPPGDSEVT
                     LLSGGERRRVALCKLLLQKPDLLLLDEPTNHLDAESVLWLEQHLAQYPGAVLAVTHDR
                     YFLDNVAQWIAEVDRGRLYPYEGNYSTYLEKKGERLQIQGKKDVKLAKRLRDELEWVR
                     SNAKGRQVKSKARLARYEEMAAEADRTRKLDFDEISIPPGPRLGSQVIEAHKLGKGYG
                     DRMLIENLSFTLPRNGIVGVIGPNGVGKTTLFKTIMGLEEADAGTVRVGETVKISYVD
                     QARAGLDPTKTLWETVSDGLDFIKVGHVEIPSRAYVSQFGFKGPDQQKPTGVLSGGER
                     NRLNLALTLKQGGNLLLLDEPTNDLDVETLGSLGNALLEFPGCAVVISHDRWFLDRVA
                     THILAYEGDEENPARWHWFEGNFESYETNKVERLGADAARPHRVTYRKLTRD"
     gene            3810..>4088
                     /locus_tag="VHM65_08105"
     CDS             3810..>4088
                     /locus_tag="VHM65_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2705707.1"
                     /translation="MRLGLQVSYFTWPGAPDSIGPTFGRIAREADEAGLYSLWVMDHF
                     FQISVIGPPELDMLEGYAALAFAAGQTSRIRLGTLVTGVTYRHPGILVK"
BASE COUNT          693 a         1382 c         1399 g          614 t
ORIGIN      
        1 ccacatgctc ggcggctcat cgcggcgcgc ctcgcaacgg cgatcggtgc cgtcgccgcc
       61 gagcatgtgg tcggtcccgt ccgcgcggtc ctggcgcggc acgcccgaac ccgggacctg
      121 ctcgacgaag ccaggctggc gtccccaccg ggctagctca gggctggccc ttgcgcgcca
      181 ggtatgacct acatcgagat cggctgaggc agccccggtc atctcagggg cggtctgcct
      241 gctgcctctc ccacgctgcc aagcgccgct cgtagacgcg taggtgcagg acggccgagt
      301 ccagcaaggg cgtgggaacc gccaggtttc gcgctcggaa tgtgaggtcg ccgaggatgt
      361 gctcgacctc ggtgtgccgt tgggtggcca gatcacgaaa catcgaggag gtgagcgggg
      421 agccggtctc ggtgatcatc gtggcggtgg taccgagctc gactgggggc agctggtagc
      481 cggcggcgga cgcaacggcg gccgcctcag cgaggatgcc ggtggccagg tcgcggccgc
      541 cgggctcggc ggcgatgtcg ccgacgctgc tgcgcatcag gcaggtcagc gcgccgagcg
      601 tggcgatgaa gacccacttg tgccacattc cggccatgat gttgtcgctg atgccgacgt
      661 caaagcccgc cccgtcgagc gcggcgtgag cggcctgggc tcccgaggtg tcggggccgt
      721 cgagagcgcc gaacagcaga cttgcgccgg gcgcgacctg aacgatgtcg ccttcctcgt
      781 tgagctgcgt cgcgaccttg acgaccccgc ccatgacggt gctggggccg aaccgcgcct
      841 ggagggcgtc gagatggccg atgccgttga ggaatggcac gatcatcgtc tgcggtccga
      901 ccgccggggc gaggtcatcg atggcggacc gcagagcggt tgccttgacc gagagcacca
      961 cgagatcgaa cgtctcggtg agctggtcgg cggtcaccag ctgcggctgc actgcccagg
     1021 cgtcgccggt ggcgacaatg cgcagccctc tggttcgcaa gacctcggcg cggtgcgggt
     1081 gcacgagaaa ggtgacgtcg cggccggccc gggtcagcag ggcaccgaag tagcctccag
     1141 tcgcgccggc acccacgatg agaatcttca ccgtcgttcc tttcgttgac gtacgttcaa
     1201 ctggaccggc acgagggccc ggaggagccg tcacgcctca tgctctcagg gggagccggc
     1261 acagcgcccc tgcatctgag ctggtaacga tgacccgcga cggcgccgta tcgacgacag
     1321 tgcacatccc ctccggactc ggtcccactc tccacagagc agggactggc cctgccgtcg
     1381 cgagccccgt catacccatg ctggcttcac cgcctcaccc gagcgcggca gacatggaag
     1441 gcaggaccag atgaacgaga gcttcgtgac cgtctgcggc aacgtcgtcg cgaaaccaga
     1501 ggttcgcacg gccaagaccg ggacgcagtt tgccaccttc cgggtggcct cgacgagccg
     1561 gcgcaagagc caggacggtt cggcctttgt cgatgggccg accaacttcg tcaacgttgt
     1621 cgcgttccgc gggctgggcg ccaacgtgac ggccagcctt gagcgtggcc acccggtcat
     1681 cgtctacgga cggctgcgcg tcaagcagtg gaccaaggac gaccggtcgg ggacgtcggt
     1741 cgagatcgac gcctacaacg tcggccacga cctcaaccgc gggaccaccg cgtactcgcg
     1801 ccagatgaga tcgctcggcg acaccaacga ccggctctct gacgaggcgg tccaggaggc
     1861 cacgcaggag ctcgaggcag cgttcatcgt cgaggacggg atggcggtgg acccgcgcac
     1921 cggcgaggtc gccgaccagc agacccggcc ggggtacttc gaggaggcaa gctagcgcgc
     1981 cgcgcctcgg ccctgaccgt gccctgagcg ttcgccgtgt tcggctcgtc cgggcccggc
     2041 cgatagcctg ctcaccatgg ccgagttcat ctacaccatg tcccgagcgc gcaaggcggt
     2101 cggcgacaag ctgatcctcg acgacgtgac gatgtccttc tacccgacag ccaagatcgg
     2161 tgtcgtcggt cctaacggcg cgggcaagtc gacaatcctc aagatcatgg ccgggctcga
     2221 ccagccgtcc aacggcgagg cacgcctgtc tccgggctac accgtgggca tcctgctgca
     2281 ggagccctcg ctcaacgagg tcaagaccgt cctgggtaac gtcgaggagg gcgtcggcga
     2341 gatcaaggcc aagctcgacc gctacaacga gatcgcgatg gagatggccg agccggacgc
     2401 cgactacgac aagctcggcg acgagatgag cgcgctgcag gacgacatcg accacgccaa
     2461 cgcgtgggac ctggacagcc ggctcgagca ggcgatggac gccctgcgct gcccgcccgg
     2521 cgactccgag gtcaccctgc tctccggtgg tgagcggcgc cgggtcgccc tgtgcaagct
     2581 gctcctgcag aagcccgacc tgctgcttct cgacgagccg accaaccacc tcgacgccga
     2641 gtcggtgctc tggctcgagc agcacctggc ccagtacccc ggggcggtgc tcgcggtcac
     2701 ccacgaccgg tacttcctcg acaacgtcgc gcagtggatt gccgaggtcg accgcggtcg
     2761 gctctacccc tatgaaggca actactcgac ctacctggag aagaagggtg agcgcctcca
     2821 gatccagggc aagaaggacg tcaagctcgc caagcgcctg cgggacgagc tcgagtgggt
     2881 ccggtccaac gcgaaggggc gccaggtcaa gagcaaggct cgacttgccc gctacgagga
     2941 gatggcagcg gaggccgacc ggacccgcaa gctggacttc gacgagatct cgatcccgcc
     3001 gggcccgcgc ctgggctccc aggtcatcga ggcccacaag ctgggcaagg gctacggcga
     3061 ccggatgctc atcgagaacc tgtcgttcac cttgccgcgc aacgggatcg tcggcgtcat
     3121 cggtcccaac ggggtcggca agaccacgct gttcaagacg atcatggggc tcgaggaggc
     3181 tgacgcgggc accgtgcgcg tcggggagac ggtcaagatc tcgtatgtcg accaggcgcg
     3241 ggccggactc gaccccacca agacgctgtg ggagacggtg tcagacggcc tcgacttcat
     3301 caaggtcggg cacgtcgaga ttccctcccg tgcctacgtg tcgcagttcg ggttcaaggg
     3361 tccggaccag cagaagccaa ccggggtgct gtccggcggt gagcgcaacc ggctcaacct
     3421 cgcgctcact ctcaagcagg gcggcaacct gctcctgctc gacgagccga cgaacgacct
     3481 cgacgtcgag acgctgggct cgttggggaa cgccctgctc gagttccccg gttgtgccgt
     3541 cgtcatcagc cacgaccggt ggttcctgga ccgcgtcgcg acgcacatcc tcgcctacga
     3601 gggcgacgag gagaacccgg cgaggtggca ctggttcgag ggcaacttcg agtcctacga
     3661 gaccaacaag gtcgagcggc tcggtgcgga cgcggcccgt ccgcaccggg tgacctaccg
     3721 caagctcact cgcgactgag agcgcgggtc ttgtccctcg cgacggcccc tgcgttgccc
     3781 aaggccgttg cggggggtga cactggggca tgcgactcgg actgcaggtc tcctacttca
     3841 cctggccagg cgcccccgac tccatcgggc cgaccttcgg gcggattgcc cgggaggccg
     3901 atgaggcggg gctgtactcg ctgtgggtga tggaccactt cttccagatc tcggtgatcg
     3961 gcccacccga gctggacatg cttgagggct atgccgccct cgccttcgcg gcggggcaga
     4021 cctcgcgcat tcgtctcggg accctggtga ccggggtgac ctaccggcac cccggcatcc
     4081 tcgtcaag
//
LOCUS       DATAIX010000545         2615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_221394, whole genome shotgun sequence.
ACCESSION   DATAIX010000545 DATAIX010000000
VERSION     DATAIX010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221394"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VHM65_08110"
     CDS             complement(<1..407)
                     /locus_tag="VHM65_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705708.1"
                     /translation="MAHDRSILGTADITDDQLAGVVAEEHGVDPGAVTVIQSTAEEVP
                     YDLESMTTAGRYWVHGTARVDDSEMPFRYFVKHVQSWSRSPLFRFVPEPFQAQAEAGV
                     PWRTEPLGQVGPVDQRLGAPGDAGLGLGLERLR"
     gene            566..1429
                     /locus_tag="VHM65_08115"
     CDS             566..1429
                     /locus_tag="VHM65_08115"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX2705709.1"
                     /translation="MSTPTTAGQAGLDNPATDPREVAEAAAAVIAERSGARTHDIALV
                     LGSGWGQTGDLIGETVATIPNEEVPGFAKAAVAGHSGSMRSVAVGDTGKRALVYGTRT
                     HYYEGRGVRAVVHGVRTAAAAGCRTVVLTNGCGGLNPVWSPGTPVLIKDHLNLTAVSP
                     LEGATFVDLTDLYAVRLRAIAHEVDAELDEGVYVQFRGPQYETPAEVQMARILGGDLV
                     GMSTTLEAIAAREAGLEILGISLVTNLAAGISPVPLSHAEVLEAGRAAAPRCGRLLAE
                     VVARIVSEPRA"
     gene            1426..>2615
                     /locus_tag="VHM65_08120"
     CDS             1426..>2615
                     /locus_tag="VHM65_08120"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEX2705710.1"
                     /translation="MRASSRPLPELVAAARDWITDDPDPMTRDELAEVVEAASSGSAG
                     ATADLADRFSGLLAFGTAGLRGALGAGPNRMNRVVVIRAAAGLTAYLQEIGIPAPHVV
                     IGYDARHNSDIFAADTAAVITAAGGSAVVLPRALPTPVLAFAIRHLGADAGVMVTASH
                     NPSQDNGYKVYLGDGSQIVPPADSGIAAQIDAKPQVRDIPRATDGWQVLGEDVFDAYI
                     TAVATVVAPDSPRAVTVVHTSLHGVGNETILAAFARAGFPEPIVVAEQAEPDPDFPTV
                     HFPNPEEPGAIDAAIELAEQTNPDLVVANDPDADRCGAATWVQGGWRMLRGDELGAVL
                     GAHLLARGVPRDAVFANSIVSSRLLAAMAASAGVRHEETLTGFKWISRVPGLTYGYEE
                     ALGY"
BASE COUNT          359 a         1003 c          895 g          358 t
ORIGIN      
        1 tgccggagcc gttccaggcc caggccgagg ccggcgtccc ctggcgcacc gagccgctga
       61 tctaccggtc cgacctggcc cagcggctcg gtgcgccagg ggacgccggc ctcggcctgg
      121 gcctggaacg gctccggcac gaaccggaag agcggcgacc ggctccacga ctggacatgc
      181 ttgacgaagt agcggaaggg catctcgctg tcgtcgaccc gggccgtccc gtggacccag
      241 tagcgcccgg cggtcgtcat gctctccagg tcgtaaggca cctcctcagc tgtcgactgg
      301 atgaccgtca cggcccccgg gtcgacgccg tgctcctcgg cgacgacgcc ggcgagctgg
      361 tcgtcggtga tgtcagccgt ccccagaatg ctccggtcgt gcgccatgtc ccgtccccct
      421 ccgccctggc cagtcggttc cagccactgt cgggccagtg cgcgcatccg gtcaactgat
      481 taccgggagc cgactgggta tcggccggta cgagcccaac ccgcgaggcc atcggcaggg
      541 atcgcccgcg caataggctg ccggtatgtc cacgccaacg acggccggcc aggccggtct
      601 cgacaacccg gcgaccgacc cccgggaggt ggccgaggcg gcagccgcgg tcatcgccga
      661 gcgctcgggc gctcgcaccc acgacatcgc gctcgtgctc ggctcgggct ggggccagac
      721 gggtgacctc atcggcgaga cggtcgccac gatccccaac gaggaggtgc cggggttcgc
      781 caaggccgcg gtggcggggc actccgggtc gatgcgttcg gtcgccgtcg gtgacaccgg
      841 caagagagcc ctggtctacg gcacccgcac gcactactac gagggccgag gagtccgcgc
      901 ggtcgtccac ggtgtccgga ccgccgccgc cgccgggtgc cgcacggtcg tgctcaccaa
      961 cgggtgcggg ggtctgaacc cggtgtggtc ccctggcacc cctgtgctca tcaaggacca
     1021 tctcaacctc accgccgtgt caccgctcga gggcgccacc ttcgtcgacc tcaccgacct
     1081 gtatgccgtg cggctgcggg cgatcgcgca cgaggtcgac gccgagctcg acgagggcgt
     1141 ctacgtccag ttccgcggac cgcagtacga gaccccggcc gaggtccaga tggccaggat
     1201 cctcggcggc gacctcgtcg ggatgtcgac cacactcgag gcgatcgccg cccgcgaggc
     1261 gggcctggag atcctcggca tctcgctcgt gaccaacctc gccgccggta tctcccccgt
     1321 gcccctctcc catgcggagg tcctcgaggc ggggcgggct gcggcgccgc gctgcggtcg
     1381 cctgctcgcc gaggtcgtcg cgcgcatcgt gagcgagccc cgggcatgag ggccagctcg
     1441 cgcccgctgc ccgagctcgt cgccgccgcg cgcgactgga tcaccgacga cccggacccg
     1501 atgacccggg acgagctcgc cgaggtcgtc gaggcggcgt ccagcgggtc ggccggggcc
     1561 accgccgatc tggccgaccg tttctcgggc ctgctcgcct tcgggacggc cgggctccgc
     1621 ggagccctcg gcgccggacc gaaccggatg aaccgggtcg tcgtcatccg cgcggccgcc
     1681 gggctcacgg catacctgca ggagatcgga atcccagccc cgcacgtcgt catcgggtac
     1741 gacgcgcgcc acaactccga catcttcgct gcggacaccg cggccgtcat cacggcggcg
     1801 ggcggcagcg ccgtggtcct gccccgggcg ctgccgacgc ccgtgctcgc cttcgcgatc
     1861 cgccacctcg gcgctgatgc cggggtcatg gtcaccgcca gccacaaccc gtcccaggac
     1921 aacggctaca aggtctatct cggcgacggg tcgcagatcg tcccccccgc agactcgggg
     1981 atcgccgcgc agatcgacgc caagccacag gtccgtgaca tccccagagc caccgacggc
     2041 tggcaggtcc tcggtgagga cgtcttcgat gcctacatca cggccgtggc aaccgtcgtc
     2101 gccccggaca gcccccgcgc cgtcaccgtc gtccacacct cgctgcacgg cgtcggcaac
     2161 gagaccatcc tcgcggcgtt cgcccgcgcc gggttccccg agccgatcgt cgtcgccgag
     2221 caggccgagc ccgaccccga cttcccgacc gtgcacttcc ccaaccccga ggagccgggc
     2281 gcgatcgacg cggccatcga gctcgccgag cagaccaacc ccgacctcgt cgtcgccaac
     2341 gaccccgacg ccgaccggtg cggggcagcc acctgggtcc agggcggctg gcggatgctg
     2401 cgcggcgacg agctcggcgc ggtgctcggg gcgcatctgc tggcgcgggg cgtcccgcgg
     2461 gacgcggtat tcgccaactc gatcgtctcg tcccggctgc tcgccgccat ggcggcctcc
     2521 gccggggtgc gccatgagga gacgctgacc ggtttcaagt ggatctcgcg ggtgccgggg
     2581 ctcacctacg gctacgagga ggccctcggt tactg
//
LOCUS       DATAIX010000546         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_178576, whole genome shotgun sequence.
ACCESSION   DATAIX010000546 DATAIX010000000
VERSION     DATAIX010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178576"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2197
                     /gene="gyrA"
                     /locus_tag="VHM65_08125"
     CDS             <1..2197
                     /gene="gyrA"
                     /locus_tag="VHM65_08125"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX2705711.1"
                     /translation="SMEMVRDIDEETVDFVPNYDGKTMQPSVLPSRFPNLLINGSSGI
                     AVGMATQIPPHNLREVAGGALWYLANPEVGRAELLEKLLTVVKGPDFPTGALIMGRKG
                     IEDAYRTGRGSIIMRAVVSVDEIRGRQCLIVTELPYQVNRDSLAAKIAELVKDGRLQG
                     IADIEDQTSGRTGQRLVIVLRRDAVAKVVLNNLYKHTQLQTTFGANMLALVDGVPRTL
                     PLDAFIRHWVTHQVDVIVRRTTYRLRKAEERIHILRGLLKALDALDEVIALIRRSRTV
                     ENARDGLMAFLEIDELQAQAILDMQLRRLAALERQKTIEQHDEQQAIIDDCQDILAKP
                     LRQREIISTELTGIVDKFGDDRRTSIEFFEGDMSVEDLIPEEDVVVTITRGGYVKRTK
                     VEMYRSQHRGGKGVKGAALRFEDVVEHFFTTTTHHWLLFFTNLGRVYRAKAYELPEGS
                     RDAKGQHVANLMAFQPGETIAQVLAISDYSSAPYLVLATRAGLVKKTRLAEYDSPRTG
                     GLIAVNLREGDELVGAELASAQDDLLLVSRKGQSVRFTASDDALRPMGRATSGVTGMK
                     FRRGDHLLAMSVVTQGTEPDVFVVFENGLAKRTPVGEYRVQGRGGLGIQVAKLSDKGG
                     DLVGALTVTAEDEVMVVMEKGKIVRSRVDEVRHTGRSTMGVIFATPDRGDAIVAVARN
                     AEHVPEVTEGADGTPAPMDSADAGPAQDDADGVASSGRADEADDAGGAE"
BASE COUNT          383 a          794 c          758 g          331 t
ORIGIN      
        1 gtccatggag atggtccgcg acatcgacga ggagaccgtt gacttcgtcc cgaactacga
       61 cggcaagacg atgcagccgt cggtcctgcc gagccggttc ccgaacctgc tcatcaacgg
      121 ctcgtccggg atcgcggtcg ggatggccac ccagatcccg ccgcacaacc tgcgcgaggt
      181 tgccgggggc gctctgtggt atctcgccaa ccccgaggtc ggccgcgccg agctgctcga
      241 gaagctgctc accgtcgtca agggccccga cttcccgacg ggcgcgctca tcatggggcg
      301 caagggcatc gaggacgcct accggaccgg gcgtggctcg atcatcatgc gggcggtcgt
      361 cagtgtcgat gagatccggg gccggcagtg cctcatcgtc accgagctgc cctaccaggt
      421 caaccgggac tcgctcgccg ccaagatcgc cgagctcgtc aaggacggcc ggctccaggg
      481 catcgccgac atcgaggacc agacctcggg ccgcacgggt cagcgcctcg tcatcgtgct
      541 ccggcgcgac gccgtggcca aggtcgtcct caacaacctc tacaagcaca cccagctgca
      601 gacgacgttc ggcgccaaca tgctcgccct cgtcgacggc gtgcctcgca cgctgccgct
      661 cgacgcgttc atccggcact gggtcaccca ccaggtcgac gtcatcgtgc ggcgcacgac
      721 ctaccggctg cgcaaggccg aggaacgcat ccacatcctg cgcgggctgc tcaaggccct
      781 cgatgcgctc gacgaggtca tcgccttgat ccggcggtcg cggaccgtcg agaacgcccg
      841 tgacggcctc atggcattcc ttgagatcga cgagctgcag gcccaggcga tcctcgacat
      901 gcagctgcgc cggctcgccg cgctcgaacg gcagaagacg atcgagcagc acgacgagca
      961 gcaggcgatc atcgacgact gccaggacat cctggccaag ccgctgcgcc agcgagagat
     1021 catctcgacc gagctcaccg ggatcgtcga caagttcggc gacgaccgcc gcacgtcgat
     1081 cgagttcttc gagggcgaca tgagcgtcga ggacctcatc cccgaagagg acgtcgtcgt
     1141 gacgatcacc cgcggcggct acgtcaagcg gaccaaggtc gagatgtacc gctcccagca
     1201 ccgcggcggc aagggcgtca agggtgccgc gctgcgcttc gaggacgtcg tcgagcactt
     1261 cttcacgacg acgacccacc actggctgct cttcttcacc aacctcggcc gggtctaccg
     1321 ggccaaggcc tacgagcttc cggagggctc acgcgacgcc aagggacagc atgtcgccaa
     1381 cctcatggcc ttccagccgg gcgagacgat tgcccaggtg ctggccattt cggactactc
     1441 ctccgcgcca tacctcgtgc ttgccacccg cgccgggctg gtcaagaaga cgcgcctggc
     1501 cgagtacgac tcgccgcgca ccggggggct catcgcggtc aacctgcgcg agggtgacga
     1561 gctcgtcggg gccgagctcg cgtcggcaca ggacgacctg ctcctggtca gccgcaaggg
     1621 ccagtcggtg cggttcaccg cctccgacga cgcactgcgc ccgatggggc gcgcgacgtc
     1681 cggtgtcacc gggatgaagt tccgtcgcgg cgaccacctg ctcgccatgt cggttgtcac
     1741 gcagggcacc gagcccgacg tgttcgtcgt cttcgagaac ggcctcgcca agcggacgcc
     1801 ggtgggggag taccgcgtcc agggccgtgg cggcctcggc atccaggtgg ccaagctctc
     1861 cgacaagggc ggcgatctcg tcggcgcgct gacggtcacg gccgaggacg aggtcatggt
     1921 cgtcatggag aagggcaaga tcgtgcgctc gcgcgtcgac gaggtccgcc ataccggccg
     1981 gtcgacgatg ggcgtcatct tcgcgacgcc ggaccgcggt gacgctatcg tggcggtcgc
     2041 gcgcaacgcc gagcacgtcc ccgaggtgac ggagggcgcc gacggcacgc cggctcccat
     2101 ggattcggcc gacgccggac ctgctcagga cgatgccgat ggcgtagcgt cttcgggaag
     2161 ggccgacgag gccgatgatg ctggaggtgc cgagtgagtg cgacggaccg ggacgacacg
     2221 gcgacgtccg agctgcccca gctcggcgac ccggcgtcct tcgagt
//
LOCUS       DATAIX010000547         3370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_59419, whole genome shotgun sequence.
ACCESSION   DATAIX010000547 DATAIX010000000
VERSION     DATAIX010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59419"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1972
                     /locus_tag="VHM65_08130"
     CDS             <1..1972
                     /locus_tag="VHM65_08130"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX2705712.1"
                     /translation="SYSLEALKRDFGQEVAMLVDGVTKLDKVTYGEAAQAETVRKMVV
                     AMARDIRVLVIKLADRLHNARTWRYVSPESAARKAKETLEIYAPLAHRLGMNTIKWEL
                     EDLSFAALYPKVYDEIVRMVAERAPAREEYLFGVCSEVERDLKAAKIRAIVTGRPKHY
                     YSVYQKMVGRGREFDEIYDLVAVRVLVDSVRDCYAALGSLHARWKPVPGRFKDYIAMP
                     KFNMYQSLHTTVIGPEGKIVEIQIRTHLMHRRAEYGVAAHWKYKEDPSAVGPAPKGAD
                     TGPINDMAWLRQLLDWQRETEDPGEFLDSLRFEINAAEVYVFTPKGAVVSLPSGSTPV
                     DFAYAVHTEVGHHCIGARVNGRLVPLESRLDNGDVVEVLTSKADGAGPSRDWLTFVRS
                     ARARNKIRQWFSKERREEAIESGKEAIARAMRKQGLPLQRLLTAESLSSLAIELRYQD
                     ISGLYAAVGENHVSAQTVISRLLASLGGEDGASEDVAEATTPSRAIRRRSNDPGVVVE
                     GSADVWVKLARCCTPVPGDSILGFVTRGNGVSVHRSDCTNAESLKAQQDRLIAVEWAP
                     SADSVFLVQLQVEALDRARLLSDTSRVLSDQRVNILSASVQTTRDRVANLRFTFEMGD
                     PAHLEHVIQAVRKVDGVYDVYRVTGRAKDPPD"
     gene            complement(2002..>3370)
                     /locus_tag="VHM65_08135"
     CDS             complement(2002..>3370)
                     /locus_tag="VHM65_08135"
                     /inference="COORDINATES: protein motif:HMM:NF015926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HEX2705713.1"
                     /translation="AVPEVEAVPEPAPVVAAAPVRPGAPMPSPAMLAARHGAPSAAAF
                     GRVDESGVVYVKTGDGEREVGSYPGVSPREALAYFGRKYDDLSAQVDLLLQRVTHTEM
                     AAKEADEAHSKLVALSTEPNVVGDLAELSAKVARIGDAVRARPAAEATARAAARAAAT
                     TAREELVAEAERIAAQPEERVQWKTSGERMRELLDAWKSHQRSGPRIDKETEAALWHR
                     FGAARNSFDKARRVHFAHLDEFRGDAKLAKERITTEAEKLATSRDWPATASEFKRLMD
                     RWRQAGRAGRSDDDALWARFKAAQDSFFEAKDALVAAEDEVFRASLAVKDGLLAEAEA
                     ILPVTDLEAAKAALRSIQDRWDKAGKVPRADMDRTERSLKRVEQAVRDIEEKRWASSN
                     PEAAARARSLVDQLETAVAGLERELAAAQASGNTAKVATAQAALDARRQWLEQARAGL
                     AEFGG"
BASE COUNT          485 a         1198 c         1187 g          500 t
ORIGIN      
        1 gtcatacagc ctggaggcgc tcaagcgcga cttcgggcag gaagtcgcca tgctcgtcga
       61 cggcgtgacc aagctcgaca aggtcaccta cggcgaggcc gcgcaggccg agaccgtgcg
      121 caagatggtc gtcgcgatgg cgcgcgacat ccgggtgctc gtcatcaagc tcgccgaccg
      181 gctccacaac gcccggacct ggcgctacgt ctcgccggag tcggcggcac gcaaggccaa
      241 ggagaccctg gagatctacg ccccactggc ccaccggctc ggcatgaaca ccatcaagtg
      301 ggagctcgag gatctctcct tcgcggcgct ctaccccaag gtgtacgacg agatcgtgcg
      361 gatggtggcc gagcgggcgc ccgcgcggga agagtacctc ttcggggtct gcagcgaggt
      421 cgagcgcgac ctcaaggccg ccaagatccg cgcgatcgtc accgggcgcc ccaagcacta
      481 ctactccgtc taccagaaga tggtcgggcg tggccgcgag ttcgacgaga tctacgacct
      541 cgtcgccgtc cgggtcctcg tcgactcggt gcgggactgc tacgccgcgc tcggctcgtt
      601 gcatgcgcgg tggaagccgg tcccgggccg gttcaaggac tacatcgcca tgccgaagtt
      661 caacatgtac cagtcgctgc acacgacagt catcggaccc gagggcaaga tcgtcgagat
      721 ccagatccgc acccacctca tgcaccggcg ggccgagtac ggcgtcgcgg cccactggaa
      781 gtacaaggag gacccgagtg cggtggggcc ggcccccaag ggcgcagaca ccgggccgat
      841 caacgacatg gcctggctgc gccagctgct cgactggcag cgggagaccg aggacccggg
      901 ggagttcctc gactcgctgc ggttcgagat caacgcggcc gaggtctacg tcttcacgcc
      961 caagggtgcg gtcgtgtcgc tcccgtcggg gtcgaccccg gtcgacttcg cctatgccgt
     1021 ccacaccgag gtgggtcacc actgcatcgg tgcccgggtc aacggccggc tcgtgccgct
     1081 ggagtccagg ctcgacaacg gtgatgtcgt cgaggtcctg acctcgaagg ccgacggggc
     1141 cggaccgtcg cgcgactggc tcaccttcgt gcgaagtgcg cgggcccgca acaagattcg
     1201 tcagtggttc tccaaggagc gtcgcgagga ggcgatcgag tccggcaagg aggccatcgc
     1261 ccgggcgatg cgcaagcagg gcctcccgct gcagcggctc ctcaccgccg agtccctcag
     1321 ctcgctcgcg atcgagctgc gctaccagga catcagcgga ctctacgccg ccgtcggcga
     1381 gaaccacgtc tcggcgcaga cggtcatctc ccggctgctc gcctctctcg gcggggagga
     1441 tggcgcgagc gaggacgtcg ccgaggccac caccccgagc cgggcgatcc gccgccggag
     1501 caacgacccc ggggtggtcg tcgagggctc ggccgacgtc tgggtcaagc tcgcccgctg
     1561 ctgtacgccg gtgcccggtg actcgatcct cggcttcgtc acccggggca acggcgtctc
     1621 ggtccatcgc agcgactgca ccaacgccga gtcgctcaag gcgcagcagg accggctcat
     1681 cgccgtcgag tgggcgccct cggccgacag cgtcttcctc gtccagctgc aggtcgaggc
     1741 cctcgaccgg gcccgcttgc tcagcgacac gagccgggtc ctgtccgacc agcgggtcaa
     1801 catcttgtcc gcgtcggtcc agacaacccg agaccgagtg gcaaacctgc ggttcacgtt
     1861 cgagatgggc gacccggcgc atctcgagca cgtcatccag gccgtgcgca aggtcgacgg
     1921 ggtgtatgac gtctaccgcg tcaccgggcg ggccaaggac cccccggact gacctggcgg
     1981 gctgggcccc cgaggcgggt gctagccgcc gaactcggcc aggcccgcgc gggcctgctc
     2041 gagccactgc cggcgcgcgt cgagggccgc ctgcgccgtg gcgaccttgg cggtgttgcc
     2101 cgatgcctgg gccgccgcca gctcgcgctc caaaccggca accgcggtct cgagctggtc
     2161 gaccaggctc cgcgcccggg cagccgcctc ggggttgctc gacgcccagc gcttctcctc
     2221 gatgtcacgg accgcctgct cgacccgctt gagggagcgc tcggtacggt ccatgtcggc
     2281 gcgggggacc ttgccggcct tgtcccagcg gtcctggatg ctgcgcaggg cggccttcgc
     2341 ggcctcgaga tcggtgaccg gcaggatcgc ctcggcctcg gcgagcaacc cgtccttgac
     2401 cgcgaggctg gcgcggaaca cctcgtcctc ggcggcgacc agggcgtcct tggcctcgaa
     2461 gaacgagtcc tgggcggcct tgaacctggc ccacaaggca tcgtcgtcgg agcggcccgc
     2521 gcggccggcc tggcgccagc ggtccatgag gcgcttgaac tcgctcgccg tggccggcca
     2581 gtcgcggctg gtggcgagct tctccgcctc ggtggtgatg cgctccttgg ccagcttggc
     2641 gtcgccgcgg aactcgtcga ggtgggcgaa atgaacccgc cgggccttgt cgaagctgtt
     2701 gcgggcggct ccgaagcggt gccagagggc cgcctcggtc tccttgtcga tccggggccc
     2761 agaccgctgg tgggacttcc aggcgtcgag gagctcgcgc atccgctctc cgctggtctt
     2821 ccactggacg cgctcctctg gctgggctgc gatgcgctcg gcctcggcga cgagctcctc
     2881 gcgggcggtg gtggccgccg cccgcgccgc cgcgcgcgca gtggcctcgg cggccgggcg
     2941 agcgcgaacg gcgtcgccga tgcgggccac cttggccgag agctcggcca ggtcgccgac
     3001 gacgttgggc tcggtgctga gggcgacgag cttgctgtgg gcctcgtcgg cctccttggc
     3061 cgccatctcc gtgtgggtga cccgctgcag gagcaggtcc acctgggcgc tcaggtcgtc
     3121 atacttgcgc ccgaagtagg ccagggcttc ccgtgggctg acaccggggt aggagccgac
     3181 ctctcgctcg ccgtcgccgg tcttgacgta gacgactccc gactcgtcga cccggccgaa
     3241 cgccgcagcg ctgggtgccc catggcgtgc ggcgagcatg gccggggagg gcatcggggc
     3301 gccaggtcgc acgggagcag ccgcaaccac cggcgcgggc tcggggacag cctccacctc
     3361 ggggacagcc
//
LOCUS       DATAIX010000548         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_59524, whole genome shotgun sequence.
ACCESSION   DATAIX010000548 DATAIX010000000
VERSION     DATAIX010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59524"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VHM65_08140"
     CDS             complement(<1..270)
                     /locus_tag="VHM65_08140"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX2705714.1"
                     /translation="MSDPQPLFRFDPSTSPDQAQAAVLVVSMGGFIDAGHTQRLVTEA
                     LLESHRHRPVATVDLDPLYDYRGRRPVMTFDRDRWTQYDKPALTLD"
     gene            complement(365..>2180)
                     /locus_tag="VHM65_08145"
     CDS             complement(365..>2180)
                     /locus_tag="VHM65_08145"
                     /inference="COORDINATES: protein motif:HMM:NF023326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEX2705715.1"
                     /translation="SYSEPRWSASQGSAVATERVTLYGVPIVAARTIGLGRVDPAEAR
                     AMFIQRALVEGDWTTRHHFFHDNRRLVARLGELEARARRRDLMVSDEVLARFYDERLP
                     GSVVSARHFDSWWKQARRHEPGLLTFTEDLLLQASTSSLTGGDYPTTWRQGELTFAVS
                     YRFEPGAPRDGVTIHVPVEVLNQVVDAGFDWLVPGLREDLVAALIRSLPKLVRRLVVP
                     AQDHASAVLPRLRPGQGLLTDQLAVLLRERAGVVIGPGDWDWDRVPPHLRVTFAVEDA
                     LGRTLAEGRDLEALRDRAAPALARRVAAAGSSLERRGLTTWDLGDIPAQFQTPATAHQ
                     PAIHGYPALVDDGASVSLRVLPTRAEADAEHPVGVRRLLLLQTTAPWQRVLSTLTNAE
                     KLALGHNPHGSVPALLQDCLDCAVDAIVAERAGSEVRTAADFAAALDAVRTHAASRVL
                     QVVRAVEPVLALHLQVVRRLDTMTAPALAGLVADVRHQLGQLIRPGFVADTGADRLSD
                     LTRYLTGVIHRLDRAPSALAKDARLADEVAQVEDAYADLLAALRPAQRGSAAVVDLAW
                     LIEELRISYFAQTLGTARPVSVQRIGKAMATIRSAPPG"
BASE COUNT          306 a          722 c          872 g          280 t
ORIGIN      
        1 gtcgagggtc agggcgggct tgtcgtactg cgtccagcgg tcgcggtcga aggtcatgac
       61 cgggcggcgc ccgcggtagt cgtagagcgg gtcgaggtcg accgtcgcga ccgggcggtg
      121 acgatgggac tcgagcaggg cctcggtcac gagccgctgg gtgtggcccg cgtcaatgaa
      181 accgcccatg gacacgacga gcaccgccgc ctgcgcctgg tcgggcgaag tgctggggtc
      241 gaagcggaag agcggctggg ggtcggacac ggtttcctca cgaggtctgg cagtatcccg
      301 gagtgggcgt agggtgcaac gagcagtcgt cgacgacaat tccactgcca gtatgacggg
      361 cacctcaccc cggtggggcg gaccggatcg tggccatcgc cttgccgatc cgctggaccg
      421 ataccgggcg cgccgtgccg agggtctggg cgaagtagga gatccgcagc tcctcgatga
      481 gccaggcgag gtcgaccacg gcagcgctgc cccgctgggc cgggcgcagg gcggcgagca
      541 ggtcggcgta cgcgtcctcg acctgggcga cctcgtcggc caggcgcgcg tccttggcca
      601 gtgcggaggg ggctcggtcg agccggtgga tgacgccagt gaggtatcgg gtgaggtcgc
      661 tcaggcggtc ggcgccggtg tcggcgacga agccgggccg gatcagctga ccgagctggt
      721 gccggacgtc ggcgacgagg cccgcgagcg ccggtgcggt catcgtgtcg agccggcgga
      781 cgacctgcag gtgcagggcc agtacgggtt cgacggctcg gacgacctgg agcacccggg
      841 aggcggcgtg ggtccggacc gcgtcgagcg cggccgcgaa gtctgcggcg gtgcggacct
      901 cggacccggc ccgctcggcg acgatcgcgt cgacggcgca gtcgagacag tcctggagca
      961 gcgccgggac gctgccgtgg gggttgtggc cgagggcgag cttctcggcg ttcgtcaggg
     1021 tcgagagcac gcgctgccag ggcgccgtgg tctgcagcag caggagccgg cgcacgccga
     1081 cggggtgctc ggcgtcggct tcggcccggg tcggcaggac ccgcagggac accgaggcgc
     1141 cgtcgtcgac cagcgcgggg tagccgtgga tggcgggctg gtgcgcggtc gcgggggtct
     1201 ggaactgcgc tgggatgtcg cccaggtccc acgtcgtgag gccgcgccgc tccaggctcg
     1261 accccgcggc ggcgacgcgg cgggccagcg caggggcagc ccggtcgcgc agcgcctcga
     1321 ggtcccggcc ctcggcgagg gtccggccca gggcgtcctc gacggcgaag gtgacgcgca
     1381 ggtggggtgg tacgcggtcc cagtcccagt cgccgggtcc gatgacgacg ccggcccgct
     1441 cgcgcaggag cacggcgagc tggtcggtga gcagcccctg ccccggtcgc agccgcggca
     1501 ggacggccga cgcgtggtcc tgcgcgggga cgacgagacg ccggacgagc ttgggcagcg
     1561 agcggatcag cgcggccacg aggtcctcgc gcaaccccgg cacgagccag tcgaaacccg
     1621 cgtccaccac ctggttgagc acctcgacgg gcacgtggat agtgacgccg tcgcgcggcg
     1681 cgccgggttc gaaacggtag gacaccgcga aggtcagctc accctggcgc cacgtcgtcg
     1741 ggtagtcccc gcccgtgagc gaggaggtgc tcgcctgcag cagcaggtcc tcggtgaagg
     1801 tgagcaggcc gggctcgtgg cgccgggcct gcttccacca cgagtcgaaa tgccgtgccg
     1861 acacgacgga cccgggcagc cgctcgtcat agaaccgcgc gagcacttcg tcgctgacca
     1921 tgaggtcccg ccgccgtgcc cgggcttcga gctcgccgag ccgggcgacg agacgccggt
     1981 tgtcgtggaa gaagtggtgc cgcgtcgtcc agtccccctc gacgagcgcc cgctggatga
     2041 acatcgcccg ggcctcggcg gggtcgaccc gcccgagacc gatcgtgcgc gcggcaacga
     2101 tcgggacgcc atacaaggtg acccgttcgg tggctacggc gctgccctgg ctcgccgacc
     2161 agcgcggttc ggagtacgag
//
LOCUS       DATAIX010000549         2242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_29147, whole genome shotgun sequence.
ACCESSION   DATAIX010000549 DATAIX010000000
VERSION     DATAIX010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29147"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VHM65_08150"
     CDS             complement(<1..381)
                     /locus_tag="VHM65_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705716.1"
                     /translation="MTDIRIEPVDIASRAPRDLAPATDRAERRTDDSPVQVRAVLRSC
                     WGLLLRDVGVLRHNVGEFLLRTLVQPVLFIFVFAYLFPRIGQQIGGGSDRFADVLIPG
                     LIAVTAVFSGVSTVSLPLAIEFGAT"
     gene            complement(374..1324)
                     /locus_tag="VHM65_08155"
     CDS             complement(374..1324)
                     /locus_tag="VHM65_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2705717.1"
                     /translation="MRSPAGPAVSITRLVKKYPKSDTPAVDGLDLEVSDGETLALLGP
                     NGAGKSTTVGICTTRTRPSAGAVRVAGWDVAHDGLRVRRNIGVVTQHNTLDRSCTVFD
                     NLYYHCRFFGFGRAAARERAGQLLEQFALAPRAGFKTAWLSGGMVQRLQIARAVAHRP
                     QVLFLDEPTAGLDPQSRLALWDQLDELRATGTTLFLTTHHMEEAERLADRVAIMDQGR
                     VLVCGTADELKRSAGNHTRVTVSVSVPHPLVRRRLLDLSCVRAVDEVDSGYVVLTSGG
                     QNSLAEIVPAASAAGITALAVREPSLEDVFIQLTRRDLRD"
     gene            1462..>2242
                     /locus_tag="VHM65_08160"
     CDS             1462..>2242
                     /locus_tag="VHM65_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2705718.1"
                     /translation="MHLTDGHPVTRHQRDDVLAWGCGPVEAIPACGLDTLVAEAIRRD
                     PGAIAVIHGDRRISYAELDAMADAIAARLRELGAGPGATVAISLGRGGHVPAALLAVL
                     RAGAAYVPVDASLPAQRVQLMIDDAAPVAAVVDATTVAAQVLATLPTVVADAIDADDR
                     SRPTVPSGPESLTGPTDLAYVLYTSGSTGRPKGVCVEHHSVVDFVFHNARAYGVGPGS
                     RVLAMASLGFDVSVAEIFTALVSGATLVVADEDDVQTPDHLA"
BASE COUNT          322 a          792 c          815 g          313 t
ORIGIN      
        1 ggtcgcgccg aactcgatgg ccagcggcag gctcaccgtc gagacgccgg aaaacaccgc
       61 ggtgaccgcg atcagcccgg ggatcagcac gtcggcgaag cggtcggagc cgcccccgat
      121 ctgctgcccg atccgcggga agaggtaggc gaagacgaag atgaagagca ccggctgcac
      181 gagcgtgcgc agcaggaact cgcccacgtt gtggcgtagc acgccgacgt cccgcagcag
      241 cagcccccag cacgagcgca ggacggcccg gacctggacg ggggagtcgt cggtacgccg
      301 ctcggcccga tccgtggccg gcgccaggtc gcgcggcgcg cggctcgcga tgtcgaccgg
      361 ctcgatgcgg atgtcagtca cgaaggtccc ttcgggtgag ctggatgaac acgtcctcca
      421 gcgacggttc acgcacggca agtgcggtga tgccggcggc gctcgcagcc gggacgatct
      481 cggccaggct gttctgcccg cccgaggtga ggacgacata cccggagtcc acctcgtcga
      541 ccgcgcgcac gcaggagagg tccagaagcc ggcgccggac aagcgggtgg ggcacgctga
      601 cgctcaccgt cacgcgggtg tggttgcccg ccgagcgctt cagctcgtcg gcggtcccgc
      661 agacgagcac gcgcccctgg tccatgatcg ccacccggtc ggcgagccgc tcggcctcct
      721 ccatgtggtg cgtggtgagg aacagcgtgg tgccggtcgc gcgcagctcg tcgagctggt
      781 cccaaagcgc gagccggctc tgcgggtcca ggccggccgt cggttcgtcg aggaacagca
      841 cctggggccg gtgggccacg gcgcgggcga tctgcagccg ctgcaccatg ccacccgaga
      901 gccacgcggt cttgaacccg gcccgcggtg ccagcgcgaa ctgttcgagc agctggccgg
      961 cgcgctcacg cgcggccgcg cggccgaagc cgaagaagcg gcagtggtag tagaggttgt
     1021 cgaagacggt gcacgaccgg tccagggtgt tgtgctgggt cacgacgccg atgttgcgcc
     1081 gtacccgcag cccgtcgtga gcgacgtccc agccggcgac ccgcaccgcg cctgcggacg
     1141 gtcgcgtccg agtcgtgcag atcccgaccg tcgtcgactt gcccgcgccg ttcggcccca
     1201 gcagggcgag ggtttcgccg tcgctgacct cgaggtcgag gccgtcgacg gccggggtgt
     1261 cggatttcgg gtacttcttg acgagccggg tgatggacac ggcagggccg gcaggtgatc
     1321 tcacgaacgg tctcctccgt tgtcgcgcgc gctcactagg gggtaggagt gccgcgaggc
     1381 cgatcgagat acgtccgcgg cccggctgat cgcgggccgg ccggatcgta tctaggaggg
     1441 gtctggccgc tcctacagct tgtgcatctg actgacgggc atccggtcac gcgccaccag
     1501 cgcgacgacg tcctcgcctg ggggtgcggg ccggtcgagg cgatacccgc ctgcgggctc
     1561 gacaccctcg tggctgaggc gatccggcgc gatcccggtg ctatcgcggt gatccacggc
     1621 gaccgccgga tctcctacgc ggaactggac gctatggctg acgcgatcgc ggcgcgcctg
     1681 cgagagctcg gtgccgggcc cggcgcgaca gtcgcgatca gcctcgggcg cgggggccac
     1741 gtgccggccg ccctgctcgc cgtcctgcgc gcgggcgcgg cgtacgtccc tgtcgacgcc
     1801 tcgctcccgg cgcagcgcgt ccagctgatg atcgacgacg cagctccggt tgcggccgtc
     1861 gtcgatgcga cgaccgttgc cgcacaggtg ctggccacgc tcccgacggt cgtggccgac
     1921 gcgatcgacg ccgacgacag gtcacggccg acggtcccga gcggacccga gagcctgacg
     1981 ggtccgaccg acctcgccta cgtgctctac acctccggca gcaccgggcg gccgaaaggc
     2041 gtctgcgtcg agcaccactc ggtggtcgac ttcgtcttcc acaacgcacg ggcgtacggc
     2101 gtcgggccgg gctcccgcgt cctcgccatg gccagcctcg gtttcgacgt gtcggtcgcc
     2161 gagatcttca ccgccctcgt gagcggagcg accctggtcg tcgccgacga ggacgacgtc
     2221 cagacgccgg accacctcgc cg
//
LOCUS       DATAIX010000550         1566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_251158, whole genome shotgun sequence.
ACCESSION   DATAIX010000550 DATAIX010000000
VERSION     DATAIX010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251158"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VHM65_08165"
     CDS             <1..888
                     /locus_tag="VHM65_08165"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF002032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX2705719.1"
                     /translation="QRRELKKLPTLRGRTIVNFFFEDSTRTRSSFEIAGKWMSADTIN
                     LSAKGSSTSKGESLRDTVMTIDAMAVDALVIRHNASGACQQVAGWVDASVINAGDGTH
                     EHPTQALLDAYTVRQRLGALDGVHVVIVGDLTHSRVFRSNVITLRTLGARVTVVAPPT
                     LMPSGIDSWATTDGFATSWDLDEVLFGGGAPPDAVMMLRVQRERMSGGYFPTAREYTV
                     CYGMTRERLDRLVAANPQVLILHPGPMNRGLEIAADAADSPRSVILDQVSSGVAVRMA
                     VLYHLLAGESPDPVGRGFG"
     gene            885..>1566
                     /locus_tag="VHM65_08170"
     CDS             885..>1566
                     /locus_tag="VHM65_08170"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2705720.1"
                     /translation="MSTSTALLTGARILGGEATDLLVRDGVIAEIGRIDRPTGEVSVI
                     DADGLVALPGFVDLHTHLREPGREDTETIRSGSAAAAVGGFTAVLAMANTSPVTDTAE
                     AAERIYDLGRACGLVDVVPVGAVTKALDGAELGELGLMARSRAQVKVFSDDGRCVQDA
                     RIMRRALEYLKLYGGVISQHSQDARLADGQACCHEGELSGRLGLPGWPGIAEEVVVAR
                     DVALARHTG"
BASE COUNT          217 a          572 c          556 g          221 t
ORIGIN      
        1 cagcgccgcg agctcaagaa gctgcccacg ctgcgcggcc gcacgatcgt gaacttcttc
       61 ttcgaggact cgacccggac gcggtcctcc ttcgagatcg ccgggaagtg gatgagcgcg
      121 gacaccatca acctgtcggc caagggctcg tcgacatcca agggcgagtc gctgcgtgac
      181 acggtcatga cgatcgacgc gatggccgtc gacgccctcg tcatccgcca caacgccagc
      241 ggcgcctgcc agcaggtcgc cgggtgggtc gacgccagcg tcatcaacgc cggtgacggc
      301 acgcatgagc acccgacgca ggccctgctc gacgcgtaca cggtgcgtca gcgcctgggg
      361 gccctcgacg gcgtgcatgt cgtcatcgtc ggggacctca cgcacagccg ggtgttccgc
      421 tccaacgtca tcacgttgcg gaccctcggg gcgcgggtca ccgtggtcgc ccccccgacg
      481 ctcatgccga gcgggatcga ctcgtgggcc acgaccgacg ggttcgcgac gagctgggac
      541 ctggacgagg tcctgttcgg cggtggcgcg ccaccggacg cggtcatgat gctgcgggtc
      601 cagcgcgagc ggatgagcgg gggctacttc ccgacggcgc gggagtacac ggtgtgctac
      661 gggatgaccc gggagcgcct cgaccggctc gtcgcggcca acccccaggt gctcatcctg
      721 cacccgggcc cgatgaaccg ggggctggag atcgccgccg acgccgccga ctccccgcgc
      781 tcggtcatcc tcgaccaggt gtcctccggg gtcgccgtgc ggatggccgt cctctaccac
      841 ctgcttgccg gggagagtcc cgacccggtc ggcagggggt tcggatgagc acgtccacgg
      901 ccctgctcac gggcgctcgg atcctcggtg gcgaggccac cgacctgctt gtccgggacg
      961 gggtcatcgc cgagatcggg cgcatcgacc ggcccactgg ggaggtcagc gtgatcgacg
     1021 ccgacggcct ggtggccctg cccggcttcg tcgacctgca cacccacctg cgcgagccgg
     1081 gccgcgagga caccgagacg atccgctcgg gctcggccgc ggccgcggtg ggcgggttca
     1141 ccgcggtcct cgccatggcc aacacgagcc cggtcaccga cacggccgag gccgccgaac
     1201 ggatctacga cctcggccgg gcctgcgggc tcgtcgacgt cgtgccggtc ggtgccgtga
     1261 ccaaggccct cgacggggcc gagctcggcg agctcgggct catggcccgc tcacgtgccc
     1321 aggtcaaggt cttctccgac gacggccgct gtgtccagga cgcccggatc atgcgccggg
     1381 cgctggagta cctcaagctc tacggcgggg tcatctccca gcacagccag gacgcccggc
     1441 tcgccgacgg ccaggcctgc tgccacgagg gtgagctctc cgggcgcctc gggctgcccg
     1501 gctggcccgg catcgccgag gaggtcgtcg tcgcgcgcga tgtcgcgctg gcccgccata
     1561 cggggt
//
LOCUS       DATAIX010000551         3573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_251185, whole genome shotgun sequence.
ACCESSION   DATAIX010000551 DATAIX010000000
VERSION     DATAIX010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251185"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VHM65_08175"
     CDS             <1..1170
                     /locus_tag="VHM65_08175"
                     /inference="COORDINATES: protein motif:HMM:NF025309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HEX2705721.1"
                     /translation="STDQAEVFVNSSAAGGLRMTVHGLTRGMTARAAHEAALGAGGIV
                     KLVTAGPIGDYDLADIRAIDPNVILLAGGVDYGDQDIVLGIAERLATSGLTAPIVYAG
                     NVAIRRQVRDIFAGAGLELIISANVFPEVDVLDVAPLRAIVHDVFNRHIIHAPGMARL
                     AGLSSWSILPTPGAVLEGAELFADAVGDCVVVDVGGATTDVHSVTDGTPEWTSRTIDP
                     EPRAKRTVEGDLGVFVNAEHVAGLAGGDAGAAGLEHLRAVPTTQEQHDLTRRLCRCAV
                     QTSLSRHAGVVTDLYTPTGRKHIVRGKDLTGVRWVVGTGGALTRVPGGEAILRSICTG
                     AGKHLLPEPGARILIDRDYRFSALGTLAQAYPDEVKATLRSWVHSTGQPIVNDSE"
     gene            1177..2256
                     /locus_tag="VHM65_08180"
     CDS             1177..2256
                     /locus_tag="VHM65_08180"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HEX2705722.1"
                     /translation="MFIRTPRLEVFADRVAANARVVTELCRRHGVEVACVTKVMGAHP
                     GLVHALEGASPAMIADSRIGNLQLISNTGSELPTMLLRLPTPSRVADVVRCADYSLNS
                     SVETIELLSRAAQLQRVDHKVVLMVDVGDLREGVWPDRLVSVLRSVAHLPRVEVVGIG
                     TNLACFGGVVPSPENMQLLVDLRTACREATDLDLAMISGGNSANLPLLASGGMPPEVN
                     HLRIGEAITLGRNVLDRSPWPGTRQDTLRLVAEVVEVERKPSLPIGQRSQDAFGGVTT
                     FIDRGIRTRAICNIGRQDVVADGVFPEDVGIFVLGASSDHLMLDVEDAAGPVRVGDEI
                     GFVPSYGSLLAATTSPYVQKVLVRG"
     gene            2895..>3573
                     /locus_tag="VHM65_08185"
     CDS             2895..>3573
                     /locus_tag="VHM65_08185"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX2705723.1"
                     /translation="MTALLRPLLLARGRIVAVADLVEAVWAGAPPADPTGALQALVAR
                     SRRLGLTITATAGGYRLDPRDLEVDSVLAQDRLVGARAARAAGDHTLATNLAAQGLAL
                     WPVDAAAPAVGASGRVFTDLLVIRVEGALAARDGAPGSAELLLDDDVIDALRDAVGHR
                     PADEPLTALLMRALAARGRESEAHAAYVRLRGELAETFGTDPAGVVAQTHLALLRGEL
                     APKSITPA"
BASE COUNT          507 a         1310 c         1233 g          523 t
ORIGIN      
        1 tccacggacc aggccgaggt gttcgtcaac agctcggccg ccggggggtt acggatgacg
       61 gtgcacgggc tcacccgggg gatgacggcc cgcgccgctc atgaggcggc gctcggtgcc
      121 ggggggatcg tcaagctcgt caccgccggg ccgatcggcg actacgacct ggccgacatc
      181 cgtgcgatcg accccaatgt catcctgctc gccggcggag tcgactacgg ggaccaggac
      241 atcgtcctgg ggatcgccga acggctggcc accagcggac tgacggctcc catcgtgtat
      301 gccgggaacg tcgccatccg ccggcaggtg cgggacatct tcgcgggagc aggcctcgag
      361 ctcatcatca gcgccaacgt cttccccgag gtcgacgtcc tcgacgtcgc gccactgcgg
      421 gcgatcgtcc acgacgtgtt caaccggcac atcatccacg ccccggggat ggcccgcctg
      481 gccggcctgt cctcgtggag catcctgccg acccccggag ccgtgctcga gggtgccgag
      541 ctgtttgccg acgcggtcgg tgactgcgtc gtcgtcgacg tcggtggggc gacgaccgac
      601 gtgcacagcg tcaccgacgg gacacccgag tggacctcgc ggacgatcga ccccgagcct
      661 cgggccaagc gcaccgtcga gggcgacctc ggagtcttcg tcaacgccga acacgtcgcg
      721 ggtctggccg gcggcgacgc cggggcagca ggcctggagc atctgcgcgc cgtccccacg
      781 acgcaggagc agcacgacct cacccgccgg ctgtgccggt gcgccgtgca gacgagcctg
      841 agccggcatg ccggggtcgt caccgacctc tacacgccga cgggacgcaa gcacatcgtg
      901 cggggcaagg atctcaccgg cgtccgctgg gtagtcggca ccggtggggc gctgacccgc
      961 gtccccggag gcgaggccat cctgcggagc atctgcaccg gggcaggcaa acacctcctg
     1021 ccggagcccg gcgcacgcat cctcatcgac cgcgactacc gcttctctgc cctggggacc
     1081 ctggcccagg cctaccccga cgaggtcaag gccacccttc gcagctgggt ccactcgacc
     1141 ggtcagccca tcgtcaacga cagcgagtga ggaaagatgt tcatccgcac ccccaggctg
     1201 gaggtcttcg ccgaccgcgt cgcggccaat gcccgggtcg tcaccgagct gtgccgccgc
     1261 cacggtgtcg aggtcgcctg cgtcaccaag gtcatgggtg cccacccggg gctggtccac
     1321 gccctggagg gcgcctcgcc ggcgatgatc gccgactccc gcatcggcaa cctccagctg
     1381 atctccaaca cgggcagcga gctgccgacg atgctcctgc ggctgcccac gccgagccgg
     1441 gtcgccgatg tcgtgcgctg tgccgactac tcgctcaact cgtcggtcga gaccatcgag
     1501 ctgctctcca gggcggccca gctccagcgg gtcgaccaca aggtcgtcct catggtcgac
     1561 gtcggcgacc tgcgcgaggg cgtctggccc gaccgcctgg tctccgtgct gcgctcggtg
     1621 gcccaccttc cgcgggtcga ggtcgtcggg atcggcacga acctcgcctg cttcggcgga
     1681 gtcgtcccga gcccggagaa catgcagctg ctcgtcgacc tgcgcaccgc gtgccgcgag
     1741 gccacggatc tggacctcgc gatgatctcg ggcggcaaca gtgccaacct cccgctgctc
     1801 gcgagcggcg ggatgccacc ggaggtcaac cacctgcgca tcggcgaagc gatcacgctg
     1861 gggcgcaacg tcctcgaccg atcgccctgg cccgggaccc gccaggacac cctgcgcctg
     1921 gtcgccgagg tcgtcgaggt cgagcgcaag ccgtcgctcc ccatcggtca gcgcagccag
     1981 gatgcctttg gcggcgtgac gaccttcatc gaccggggga tccgcacccg ggccatctgc
     2041 aacatcgggc gccaggacgt cgtcgccgat ggcgtcttcc ccgaggatgt gggcatcttc
     2101 gtcctcgggg ccagcagcga ccacctcatg ctcgatgtcg aggacgcagc cgggcctgtg
     2161 cgggtcggcg acgagatcgg tttcgtcccg tcgtacgggt cgctgctggc cgccacgacc
     2221 tcgccatacg tgcagaaggt cctcgtccgg ggctgacgac cggcaagtgc tggaaggttc
     2281 aggttacgga cctggtgctg gtcgaggctg gcccgttcct tgccgacagt cagggtgcca
     2341 ttggtggcta accgggggca ggctcacccc cggccctcgt agaaccgtgc gtgagcctct
     2401 cggtgcacac ggctcccgtc gtccagtcgt ccacggttgc ggtgaggcga tctggcagtg
     2461 ggtgagcaag ccccggtgga gcatcgcggc catgtcgagc gcttcccaag ctttcagcaa
     2521 gcgggcggcc aaggtctgtg ccgagcacgt ggtggagagc ctccgccgcc ccatggcggc
     2581 gggaccgagc gcagttctgg accgtgcgac actggcgttc cgcgccggtt gcatcccagc
     2641 cgtagacgtg ccgcgcgacg tcgacgagga ccccgagtcg ggccgcgaag agtcgcgatg
     2701 gtggcgacac gagggactcc tcgtgccgct cggctgagca ggcccgagca agacagacca
     2761 cgtggtccat ggtcactacc agcatcgccg ggccgggaaa acgacccggt ccagtcctgg
     2821 gagatgctgt atcggtgcag atcacaactc tcggacctct gacgatcggc gggcgcgccg
     2881 tcgagggatc ccggttgacc gcgctgctgc gccccctgct actcgcgcgt ggacggatcg
     2941 tggccgtcgc cgacctggtc gaggccgtgt gggcgggcgc tcccccggcc gatccgaccg
     3001 gcgcgctcca ggccctggtg gcccgctccc gccggctggg cctgaccatc accgccactg
     3061 cgggcgggta ccgcctcgac ccacgcgatc tcgaggtcga ctccgtcctg gcgcaggacc
     3121 gccttgttgg ggcgcgcgcg gcgcgcgcag cgggcgacca tacgctggcc acgaaccttg
     3181 cggcgcaggg gctcgcgctg tggcccgttg acgccgccgc cccggccgtc ggggcgtcgg
     3241 ggcgcgtgtt cacggacctt ctggtgattc gggtggaggg cgcgctcgcc gcccgcgacg
     3301 gcgccccggg ctcggccgaa ctgctcctcg acgacgacgt catcgacgcc ctgcgcgacg
     3361 ccgtcgggca ccgacccgcg gacgagcccc tcaccgccct gctgatgcgg gcgctggccg
     3421 cgcgcggccg cgagtccgag gcccacgccg cctatgtacg cctgcgcggc gagctcgccg
     3481 agactttcgg caccgacccg gccggtgtcg tggcgcagac ccacctcgct ctcttgcgcg
     3541 gagagctcgc gccgaagagc atcacccccg ccc
//
LOCUS       DATAIX010000552         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_251231, whole genome shotgun sequence.
ACCESSION   DATAIX010000552 DATAIX010000000
VERSION     DATAIX010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251231"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VHM65_08190"
     CDS             <1..397
                     /locus_tag="VHM65_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705724.1"
                     /translation="LGPGGGCSTTAKISLASAKGVGARKASLVQATTSAAKGELVVYS
                     GGASGRAAPKLAYDILTVGVRADQTPSRLHTIVDANSGATLESFDEIHNGTGNGIFVG
                     TVAIGTTAGLDRPTIFARHGAPSPKALGR"
     gene            851..1540
                     /locus_tag="VHM65_08195"
     CDS             851..1540
                     /locus_tag="VHM65_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX2705725.1"
                     /translation="MMYRLGRGVLRPVTHALYRPAIRGTENIPREGGVILASNHLSFI
                     DSFAIPLAAPREVVFIAKAEYFTGTGIGGAIRRVFFEAINAIPVDRSSSRAAQESLDA
                     ALEVLQDGGAFGIYPEGTRSRDGRLYRGRTGVAWLALTAGVPVVPVALSGTDLIQPVG
                     ARFPRLARVTVTFGEPLHFAERYAGVPQGRARRAVTDEIMDAIAALSGQERADTFNEL
                     PPGAHGPAIGT"
     gene            1714..>2277
                     /gene="recR"
                     /locus_tag="VHM65_08200"
     CDS             1714..>2277
                     /gene="recR"
                     /locus_tag="VHM65_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX2705726.1"
                     /translation="MYEGVVQDLIDELGRLPGVGPKGAQRIAFHLLATDPQDIARLVE
                     VLSEVKDKVKFCTTCGNIAQADQCRICADPRRDPTASCVVEEPKDVVAIERTREFRGF
                     YHVLGGAISPMDGIGPDDLRIRELLQRLASGEVTEVIIATDPNVQGEATATYLSRLLK
                     DVGLTVTRLASGLPVGGDLEYADEVTLG"
BASE COUNT          338 a          834 c          749 g          356 t
ORIGIN      
        1 tctcgggccc ggtgggggtt gctcgaccac tgcgaagatc agccttgcct cggccaaggg
       61 cgttggcgcc aggaaggctt cgctggtgca ggcgaccacc tcggccgcca agggcgagct
      121 ggtcgtctac tccggtggcg cctccggcag ggccgcgccg aagctggcct acgacatact
      181 cacggtgggc gttcgggccg accagacgcc aagcaggctg cacaccatcg tggacgccaa
      241 ctccggcgcc accctggagt cgttcgacga gatccacaac ggcaccggca acggcatctt
      301 cgtgggcacg gtggccatcg gcaccaccgc gggactggac agacccacca tcttcgcgcg
      361 ccacggcgcg ccttcaccga aggcgctggg tcgctgacct gctgaaacac gaccttgccg
      421 gggccctgct gcccgacccc tgcctccttc gacgccaccc caccgtggat cgatactccg
      481 ctccgcgact gggtgctgcc ccgcgtcctg gaacttacgt tcactaggag cgtgggtcag
      541 taatcggaat ctccgaggat ggcctccaat gggcggctca gatcggcctc gagtgacgtc
      601 ccatgcccca cttgagccta caagttgatc caatactggc cttcttcccc cacctgggtc
      661 tcaagaaggc attaccgacc cacgctccta gccgccgcgg gcccggcata cccctcggtc
      721 gtccatccgt cgatgggact gtgctcgatg ggattgtgct cgatgggact gtgctcgacg
      781 ggattgtgct cgatggggct gtgctcgatg aggcggtgct gccctccggt cggcgcacta
      841 tgttcggccc atgatgtacc ggctcggcag gggcgtcctc agaccggtca cccacgcgct
      901 ctaccggccg gccatccgcg gcaccgagaa catcccccgc gagggcggcg ttatcctcgc
      961 gagcaaccac ctgtcgttca tcgactcctt cgcgatcccg ctggccgccc cccgtgaggt
     1021 cgtcttcatc gccaaggccg agtacttcac cggcaccggc atcgggggcg cgatccgacg
     1081 ggtgtttttc gaggcgatca acgcgatccc cgtcgaccgc agctcctcac gggcggccca
     1141 ggagtccctc gacgccgcac tcgaggtgct ccaggacggt ggcgccttcg ggatctaccc
     1201 cgaaggcacc cgctcgcgcg acggccggct ctaccgcggc cgcaccggcg tggcctggct
     1261 cgccctcacg gcgggcgtcc ccgtcgtgcc cgtggccctg tccggcaccg acctgatcca
     1321 gccggtcggg gcgaggttcc cccgcctggc cagggtcacc gtcaccttcg gtgagcccct
     1381 gcacttcgcc gagcggtatg ccggtgtgcc ccagggccgc gcgcgccgcg ccgtcacgga
     1441 cgagatcatg gacgcgatcg ccgcactctc cggccaggag cgcgccgata ccttcaacga
     1501 gctcccgccc ggggcccatg gcccggccat cggcacctga ggcgggcatc tcggccctct
     1561 gcgccgaggg ctcacaacga gctacctcag ggcgtggcgt gttcctggtc gcgagggaga
     1621 cccggcccct ggctctggcg cggacgcagc aaaccccgag gtcgctggcc cgtcataccg
     1681 ggccggtggt ctgggaaggc taggctcgac cctgtgtacg agggcgtggt gcaggacctc
     1741 atcgacgagc tcgggcggct gcccggggtc gggcccaagg gtgcccagcg catcgccttc
     1801 cacctgctcg cgaccgaccc gcaggacatc gcgcgcctcg tcgaggtgct cagcgaggtc
     1861 aaggacaagg tcaagttctg tacgacgtgc ggcaacatcg cgcaggccga ccagtgccgc
     1921 atctgcgccg acccgcgacg tgacccgacc gcgagctgcg tcgtcgagga gcccaaggac
     1981 gtcgtcgcga tcgagcggac ccgcgagttc cgggggttct accacgtgct cggcggggcg
     2041 atcagcccga tggacgggat cggcccggac gacctgcgga tccgcgagct gctccagcgc
     2101 ctggccagcg gcgaggtcac cgaggtcatc atcgccaccg accccaacgt ccagggcgag
     2161 gcgaccgcga cctacctctc ccggctgctc aaggacgtcg gtctcaccgt gacgcgcctc
     2221 gcatcggggc tgccggtggg tggagacctg gaatacgctg acgaggtcac gttgggc
//
LOCUS       DATAIX010000553         2046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_251271, whole genome shotgun sequence.
ACCESSION   DATAIX010000553 DATAIX010000000
VERSION     DATAIX010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251271"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VHM65_08205"
     CDS             complement(<1..470)
                     /locus_tag="VHM65_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX2705727.1"
                     /translation="MSRYGAQFGPDITFLGVARCDLDEPATYADADVVIVGAPFDGGT
                     SYRSGTRFGPRAIRMTDYLPHDGSRPSLALRTDGLVDLVVVDAGDVEMTPGDIMSALG
                     VLEVAVEKVARAGAIPLVLGGDHSVAFPDAKGVANVLGHGRISMIHFDAHADTG"
     gene            complement(513..1622)
                     /gene="menC"
                     /locus_tag="VHM65_08210"
     CDS             complement(513..1622)
                     /gene="menC"
                     /locus_tag="VHM65_08210"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEX2705728.1"
                     /translation="MRLEGVELLTVHIPLVAPFRTSFGTEHVHETMLVHAITDSGEGW
                     GECVAGSTPAYSSEYLLSAADVMRRFLAPALFRLGHDLTAEGVGPALGWVKGHRMAKA
                     GLESAILDAQLRAAGQSLASYLGAVRDRVPAGVSVGIMDSLDELMTAVGGYLDQGYRR
                     IKLKIEPGWDLVPAGLVRHEFGPDLPLQVDANTAYTRDDAEHLGRLDELGLLLIEQPL
                     DEEDIIGHAQLAARVRTPICLDESIVSARSAADAIELGATSVVNIKPGRVGGYLEAVR
                     IHDVCQDRGVPVWCGGMLETGIGRAANLALAALPNFTLPGDTSASDRYFHEDLAGPFV
                     LEDGQVAVPQGPGIGVQPIAGALDRYTVSREWMSR"
     gene            complement(1645..>2046)
                     /locus_tag="VHM65_08215"
     CDS             complement(1645..>2046)
                     /locus_tag="VHM65_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705729.1"
                     /translation="SMTDGRNSGEESDRFCVVWDLDPASPAGALPAGEAPTMGRPARP
                     SPEGASGARYVVRVGERGEPVVIPAAGTGHPLLVEIPQDIEAVRVADRQLAAAWRLAV
                     RAELGERLQAGWRLTALVARSAYLVLPPGHD"
BASE COUNT          295 a          734 c          733 g          284 t
ORIGIN      
        1 tccccggtgt ccgcgtgagc gtcgaagtgg atcatcgaga tccggccgtg gccaaggacg
       61 ttcgccacgc ccttggcatc cgggaaggcg accgagtggt cgccgccgag gacgagcggg
      121 atcgctccgg cccgggccac cttctcgacc gccacctcca ggacccccag cgctgacatg
      181 atgtcccccg gggtcatctc gacatcgccg gcatcgacga ccacgaggtc gaccagaccg
      241 tcggtccgca gcgccagtga cggacgggac ccgtcgtgcg gcaggtagtc ggtcatccgg
      301 atcgcccgtg gaccgaagcg ggttccggac cggtatgacg tgcccccgtc gaacggcgcg
      361 ccgacgatga cgacgtcagc atcggcatac gtcgccggct cgtccaggtc gcaccgcgcc
      421 acgccgagga aggtgatgtc cggtccgaac tgtgccccgt agcgactcat gccggtgatc
      481 ctaggggccg cgtgcgggcc ctggctgccg cctcatcggc tcatccactc gcgcgagacg
      541 gtgtagcggt ccagggcgcc ggcgatgggc tggacgccga ttcccgggcc ctgggggacg
      601 gcgacctggc cgtcctcgag cacgaacggc ccggccaggt cctcgtggaa gtagcggtcc
      661 gatgccgagg tgtcaccggg cagggtgaag ttcggcagcg ccgccagggc caggttggcg
      721 gctcgcccga tcccggtctc gagcatcccg ccacaccaca ccgggacgcc gcggtcctgg
      781 cagacatcgt ggatgcgaac ggcctcgagg taccccccga cccggcctgg cttgatgttg
      841 accaccgacg tcgcgcccag ctcgatggcg tcggccgccg agcgcgccga gacgatcgac
      901 tcgtccaggc agatgggtgt cctcacccgc gcggccagct gggcatgacc gatgatgtcc
      961 tcctcgtcga gcggctgctc gatcaggagg agcccgagct cgtcgagccg cccgaggtgc
     1021 tcggcgtcat ctctcgtgta ggccgtgttg gcgtcgacct gcaggggcag gtccggaccg
     1081 aactcgtggc gcaccagccc ggctggaacg aggtcccagc ctggctcgat cttgagcttg
     1141 atccggcggt agccctggtc gaggtagcca cccacggcgg tcatgagctc gtcgagggag
     1201 tccatgatgc cgaccgagac ccccgccggg acccggtccc ggaccgcacc gagatagctc
     1261 gccagcgact ggccggcggc gcggagctgg gcgtcgagga tcgctgactc cagcccggcc
     1321 ttggccatcc ggtggccctt gacccagccc agggccggcc cgacgccctc ggcggtgagg
     1381 tcgtgcccga gccggaacag cgccggcgcg aggaagcgtc gcatgacgtc ggccgcgctg
     1441 aggaggtact cgctcgagta ggccggtgtg ctgcccgcca cacactcccc ccagccctcg
     1501 ccggagtcgg tgatggcatg gacgagcatc gtctcgtgga cgtgctcggt cccgaacgac
     1561 gtccggaagg gtgccacgag ggggatgtgg acggtgagga gctcgacacc ctcgagtctc
     1621 atgcgcggtg ctcctgtcct gtgctcagtc gtggccgggg ggaagaacga ggtatgccga
     1681 ccgggccacc agcgcggtga ggcgccagcc cgcctgcagc cgctccccga gctcggcccg
     1741 gacggccagg cgccacgccg ccgccagctg tcgatccgcg acgcgcacgg cctcgatgtc
     1801 ctgcgggatc tcgacgagaa gtgggtggcc ggtgccggcg gccggtatga cgaccggctc
     1861 gccgcgctcg ccgacgcgca cgacgtaccg cgcacccgag gccccctcgg gtgacggccg
     1921 ggccggccgg cccatggtgg gcgcctctcc cgccgggagc gccccggcgg gcgaagcggg
     1981 gtcgagatcc cacacgacgc agaatcggtc ggactcctcg ccgctgttgc gcccgtcggt
     2041 catgga
//
LOCUS       DATAIX010000554         1821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_29507, whole genome shotgun sequence.
ACCESSION   DATAIX010000554 DATAIX010000000
VERSION     DATAIX010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29507"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /gene="aroC"
                     /locus_tag="VHM65_08220"
     CDS             complement(<1..953)
                     /gene="aroC"
                     /locus_tag="VHM65_08220"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX2705730.1"
                     /translation="MLRWLTAGESHGPALVAVLEGLPAGVQVTTADLAGALARRRLGY
                     GRGARMRFERDEVEVLGGVRHGVTIGSPVAIRIGNSEWAKWRTVMSPDPVDPADYAAA
                     DDVNAEREIARNQALTRPRPGHADLVGMQKYGFTDARPVLERASARETAARVALGEVA
                     ARLLAQAYGIRLVSHTVAIGTAGGLVADLPTPDDAAALDADSVRAFDAAASAAMVAEV
                     EAAKKDGDTLGGVVEVLAYGVPPGLGSYVHWDRRLDAQLAAALMGIQAIKGVQVGDGF
                     EGATRRGSSAHDEMERDGDGRIRRRTGRAGGTEGGMSTGEVL"
     gene            complement(989..1660)
                     /locus_tag="VHM65_08225"
     CDS             complement(989..1660)
                     /locus_tag="VHM65_08225"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX2705731.1"
                     /translation="MSPSVLALAAAGLGVGLLLARELRTHAYRIDGDRPLRPGRPFPA
                     WVVPGLTAAVWAALAARVGGPGREAVLPAYLFFAVLAVALTWVDLDVHRLPDGLVLPA
                     YPLLGVLLVAASVGTGHWLALRDATFTGAALGLFYLLLAVPPWGGPGLGDVKLAGVIG
                     LLLGWENPWLAVLATWLAFLVSGVAAVVLVSTRRAGLRSHIAFGPGMLAGALLAPLIF
                     QSVRR"
BASE COUNT          265 a          691 c          676 g          189 t
ORIGIN      
        1 aggacctccc cggtcgacat cccgccctcg gtgccacccg cgcgccccgt gcggcgccgg
       61 atgcgcccgt cgccgtcgcg ctccatctcg tcatgggcgg acgaccctcg gcgcgtggcg
      121 ccctcgaagc cgtcgccgac ctgcaccccc ttgatcgcct ggatccccat gagggcagcg
      181 gccagctggg cgtcgaggcg ccggtcccag tggacgtagg acccgagccc gggtggcacg
      241 ccgtacgcga ggacctcgac aaccccgccg agggtgtcac cgtccttctt cgcggcctcg
      301 acctcggcca ccatggccgc cgacgccgcc gcgtcgaacg cgcgcaccga gtcggcgtcg
      361 agagcagcgg cgtcatccgg agtcggcagg tcggccacga gccccccggc cgtcccgatg
      421 gcgaccgtgt gcgagacgag ccggatgccg taggcctgcg cgagcaaccg ggcggccacc
      481 tcgccgagcg cgacccgcgc cgcggtctcg cgggccgagg cgcgctcgag gacgggtcgg
      541 gcgtcggtga agccgtactt ctgcattccg acgaggtcgg cgtgcccggg ccggggccgt
      601 gtgagcgcct ggttgcgggc gatctcccgc tcggcgttga cgtcgtcggc cgcggcgtag
      661 tccgctgggt cgacggggtc ggggctcatg acggtccgcc acttggccca ctcggagttg
      721 ccgatccgga tcgcgacggg tgacccgatc gtcacgccgt gacggacccc gccgagcacc
      781 tcgacctcgt cccgctcgaa cctcatccgg gccccgcgcc catagccgag ccgacggcga
      841 gccagcgccc cggcgaggtc agccgtcgtg acctggaccc ccgcgggcag gccctccagc
      901 accgcgacga gggcagggcc gtgcgactca cccgccgtca gccaccgcag catggccaga
      961 tcctcccatg aggcccgctg tcctgggctc agcgcctcac cgactggaag atcaggggcg
     1021 cgaggagggc gccggcgagc atgcccggcc cgaaggcgat gtgcgacctc agcccggccc
     1081 gacgggtgga aacgaggacg accgccgcga cgccgctgac caggaaggcc agccaggtgg
     1141 ccaggacggc gagccacggg ttctcccagc cgaggagcag gccgatgaca ccggcgagct
     1201 tgacgtcgcc gaggcccggg ccgccccacg gcggcactgc cagcaggagg tagaacagcc
     1261 cgagggcggc accggtgaag gtcgcatcac gcagggccag ccagtgaccg gtacccacgc
     1321 tggccgcgac gaggagcacg ccgagaagcg ggtaggccgg gaggacgagg ccatcgggca
     1381 gccggtggac gtcgaggtcg acccaggtca gggcaacggc aaggaccgcg aagaagaggt
     1441 aggccggcag gaccgcctcg cgcccgggcc cgccgacccg cgcggccagc gctgcccaca
     1501 ctgccgccgt cagcccgggg acgacccacg cggggaaggg ccggccgggc cgcagggggc
     1561 ggtccccgtc gatccggtag gcgtgggtcc gcagctcgcg agccagcagg agcccgacac
     1621 ccaggcccgc ggccgcgagg gcgaggaccg acgggctcat gtcggggggg tgcggcgagc
     1681 ctggagctcg gcgtccagcg cggcccgcat cgcccccagc ggcggctcga gaccggtcat
     1741 gagccgcacc tggacgccgg cctggtgcag cagcacctcg gtgcccgcca tcgcgacata
     1801 accgcgctcc agggcccggg t
//
LOCUS       DATAIX010000555         7003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_132096, whole genome shotgun sequence.
ACCESSION   DATAIX010000555 DATAIX010000000
VERSION     DATAIX010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 7003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7003
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132096"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="VHM65_08230"
     CDS             <1..1381
                     /locus_tag="VHM65_08230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2705732.1"
                     /translation="HTGLAVRLAVLTVAVAVMTAIIGGAAGVSIISRAGETAAVRALS
                     TIADQAQAAASTGRDPTAAQNRARRALRNVAIQVATVTASADGSADVQGDPLAVRALS
                     AADRQSLLGGADISRVQDLPLGRMLVEARATPAGGLVLAQRRSDAAAITDQGLRRALW
                     ALAAATLLAAGLGLWVAWRMTRPLRQTAEAAHALATGHRDVVVPEVGPAEVAEVAQAV
                     NSLSEALTASERRQREFLLSVSHDLRTPLTAIAGYAEALSEGDVDPAQLPAVGRVLAT
                     ESARLERMVADLLDLARLDAHEVRLDLAPTSLTRFVEDLTAVWQVRCAAAGVPFRAEG
                     LDGDAVPDRILTTDPQRLRQGVDALLENALRLSPEGAPIVLAAGLGAGRRGVAIEVRD
                     SGPGLADADLAVAFERSVLFERYRSVRPAGTGLGLAIVHRLVTRLGGTVTAGHAPEGG
                     ACFRIELPG"
     gene            complement(1399..2139)
                     /locus_tag="VHM65_08235"
     CDS             complement(1399..2139)
                     /locus_tag="VHM65_08235"
                     /inference="COORDINATES: protein motif:HMM:NF018837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEX2705733.1"
                     /translation="MSCDHDHDHHGDSAGVPWQARELTGGGFGGDRGRSDPAVLAALG
                     DGDEEALMAVLAPSRLLVPVVAVPGEAAEGPQGQVGDQRADLAVVTLTAPDGERAMPV
                     FTGVEALATWDGAARPVPVTTAAAAQGAIQDGCDVMLVDLGSAHQRVLRASMVWALAM
                     GRTWVSPHRDEHVLAAVAVAVRDEPEVTRHTCEAGHEPGVLRVVLHLRPGLDEAAVAA
                     LATRVGERLAADGELRARIDGLAFVVTS"
     gene            complement(2136..2555)
                     /locus_tag="VHM65_08240"
     CDS             complement(2136..2555)
                     /locus_tag="VHM65_08240"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX2705734.1"
                     /translation="MSRRSVESGGPWEQRYGYSRAVRSGGLVAVSGCTSVVDGTVAHP
                     DDAGAQAEVALQTATSAVEALGGTLEDIIRTRMYVVHRGDFGAVGEAHARWFGQVRPA
                     ATMVLVAGLVDPAMRVEIEVDAVLPATGAAGDRERLS"
     gene            complement(2552..3322)
                     /gene="priA"
                     /locus_tag="VHM65_08245"
     CDS             complement(2552..3322)
                     /gene="priA"
                     /locus_tag="VHM65_08245"
                     /EC_number="5.3.1.16"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR01919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase/phosphoribosylanthranilate isomerase
                     PriA"
                     /protein_id="HEX2705735.1"
                     /translation="MTPNDMTPNDLVTADPRLELLPAVDIAGGVAVQLVQGVAGSGGQ
                     FGDPWEAAKAWQDQGAEWIHLVDLDAAFGRGSNGELIASIVGRLDLQVELSGGIRDAG
                     SLATALATGCRRVNLGTAALEHPEWTAQVIAEHGDRVAVGLDVRGTTLAARGWTTEGG
                     DVWETLARLDSEGCARYVVTDVDKDGMLAGPNLALLRDVCARTDRPVVASGGVSTLED
                     IRIIRALVPEGVEGAIIGSALYRGAFTLPEALDLAGRP"
     gene            complement(3334..3975)
                     /gene="hisH"
                     /locus_tag="VHM65_08250"
     CDS             complement(3334..3975)
                     /gene="hisH"
                     /locus_tag="VHM65_08250"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015303108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX2705736.1"
                     /translation="MTRRVVVLDYGSGNVRSAVRALARVGAEVTLSADHALALAADGL
                     VVPGVGNFHACILGLRAVDGARLVDVRLAGGRPVLGVCVGMQVLFEGSTEPYARGPQE
                     GLGEWAGIVRRLQADVVPHMGWSTVCPGEGSRLFTGVEGERFYFVHSYAVTEWPPHPE
                     GGFAAVAPIVTWSEHGEPFVAAVEDGPLSATQFHPEKSGDAGAHLLENWVRSL"
     gene            complement(3972..4607)
                     /locus_tag="VHM65_08255"
     CDS             complement(3972..4607)
                     /locus_tag="VHM65_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705737.1"
                     /translation="MTDPGRPAGLLLVRGSPNAVAAWAYRGQVGVDVVPLGQGWTGVL
                     PVGLISAVAPPYHDAVAMLFNRDVPTRLRPAIGVAVAAEVGLVAVSPRRWRAIRRWAA
                     WTPGVGLTRPAHLPLAPLSAIVSASGATVPGTREDLKDVLTDRAGDARSVLVDVLTVL
                     GLPGGALFEGSTTVTSVRGVVRVDPSDRRVAGFETVAREERSWREEVEEQQ"
     gene            complement(4646..5248)
                     /gene="hisB"
                     /locus_tag="VHM65_08260"
     CDS             complement(4646..5248)
                     /gene="hisB"
                     /locus_tag="VHM65_08260"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX2705738.1"
                     /translation="MTHRTATVSRATSESSVEIRVDLDGTGESDVSTGVRFYDHMLES
                     LAKHALIDLHIRATGDIDVDAHHTVEDVAIALGQALREALGDKSGISRFGDATVPLDE
                     ALVQAVVDVAGRPYVVHSGEPEGQAYVLIGGSYAGSLTRHVFESLAHHGGLALHVRVL
                     AGRDPHHIVEAQFKAVARALRAAVARDPRVSGIPSAKGTL"
     gene            complement(5245..6393)
                     /locus_tag="VHM65_08265"
     CDS             complement(5245..6393)
                     /locus_tag="VHM65_08265"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002877.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX2705739.1"
                     /translation="MSAIADLLRPELRGRTAYGAPQLDVPVRLNTNENSYPVPGPVAA
                     AITAAVAVAAAGLNRYPDREFTALRTELAAYLRVQSGVEIDPAGIWAGNGSNEVLQHL
                     VQAFGGPGRTALGFTPAYSMHPIIAETLGTRWVDGIRGVAGPSPGAAFELDPATVVAQ
                     VREVRPQVVFLCSPNNPTGTALSLDVVEAVLDAAPGSLVVVDEADAEFARPGTASALT
                     LLFGHPLLAVTRTMSKAFALAGARLGYLAADPELVDALRLVRLPYHLSDPTQAVARAA
                     LAHAGSLLSTVEAITAQRDRMVTELVAMGLDVVPSDANFVLFGGLRDERATWRALLDG
                     GVLVRDVGIAHYLRVTAGTPQETSAFLDVMAALAPTHRRGATDSQETR"
     gene            complement(6390..>7003)
                     /locus_tag="VHM65_08270"
     CDS             complement(6390..>7003)
                     /locus_tag="VHM65_08270"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX2705740.1"
                     /translation="TEIAVLADDSADAEHVAADLISQAEHDPLAAAVLVTDSEELADA
                     VEAALARRVPATKHSERVATALAGRQSRVVLVDDVSAGLAVCNAYAAEHLEIQTRDAE
                     AVAAQVRNAGAVFVGAYAPVSLGDYAAGSNHVLPTAGCACHSSGLSVQSFLRGVHVVA
                     YDEPALREIARTVLTLAQSEDLPAHGEAVLARFGDALPDAAAR"
BASE COUNT          955 a         2611 c         2549 g          888 t
ORIGIN      
        1 ccatacgggc ctcgcggttc gccttgccgt gctcactgtg gcggtggccg tgatgacggc
       61 catcatcggc ggcgcagcgg gcgtctcgat catctcccgg gccggcgaga ccgcggccgt
      121 gcgggccctg tccacgatcg ccgaccaggc gcaggcggcc gcgagcaccg ggcgtgaccc
      181 caccgctgcg cagaaccgcg cccgaagggc actgaggaac gtcgcgatcc aggtcgcgac
      241 cgtcacggcg tcggccgacg ggtcggccga cgtccagggt gacccgctgg cagtgcgcgc
      301 gctctccgcc gccgaccgcc agtccctcct cggcggcgcg gatatctccc gggtccagga
      361 cctgccgctc gggcggatgc tcgtcgaagc ccgggcgact cccgccgggg ggctcgtgct
      421 cgcccagcga cgcagcgacg ccgccgcgat caccgaccag gggctgcggc gagccctgtg
      481 ggccctcgcg gcggccaccc tgctcgcggc cgggctcggc ctctgggtgg cgtggcggat
      541 gacccggccg ctgcgccaga ccgccgaggc ggcccacgcc ctggcgaccg gccatcgtga
      601 tgtcgtcgtc cccgaggtag ggcccgccga ggtcgccgag gtcgcgcagg cggtcaactc
      661 gctctccgag gcactcacgg cctccgagcg ccgtcagcgc gagttcctcc tgtcggtctc
      721 ccatgacctg cgcaccccgc tcacggcgat cgccggctac gccgaggcac tcagcgaggg
      781 cgacgtcgac ccagcccagc tgccggcggt gggccgggtc ctcgcgacgg agtcggcccg
      841 gctggaacgt atggtcgccg acctgctcga cctcgcgcgg ctggacgccc acgaggtccg
      901 tctcgacctg gcgccgacct cgctcacccg gttcgtcgag gacctcacgg ccgtctggca
      961 ggtgcgctgc gccgccgccg gggtcccctt ccgggccgag ggcctggacg gggacgccgt
     1021 gcccgaccgc atcctgacga ccgacccgca gcggctgcgc cagggcgtcg acgccttgct
     1081 ggagaacgcg ctgcgcctgt ctcccgaggg cgccccgatc gtccttgccg caggcttggg
     1141 cgctggccgg cgaggggtgg ccatcgaggt gcgcgactcg ggcccggggc tggccgacgc
     1201 ggacctggcc gtcgccttcg agcggtcggt gctcttcgag cgctaccgca gcgtccgccc
     1261 ggccgggacg ggactcggcc tggccatcgt ccaccgactc gtcacccgcc tcggcggaac
     1321 cgtcacggcc gggcacgccc ccgaaggcgg cgcctgtttc cggatcgagc tccccgggta
     1381 ggaccggcga gcaggcgcct acgaggtgac gacgaaggcc aggccgtcga tcctggcgcg
     1441 gagctcacca tcagcggcaa gtcgttcccc cacccgggtc gccagcgccg cgaccgcggc
     1501 ttcgtccaga ccggggcgca ggtgcagcac gacgcgcagg actcccggtt cgtgccccgc
     1561 ctcgcaggta tggcgggtca cctcgggttc gtcacggacg gcgaccgcca ccgcggcaag
     1621 gacgtgctcg tcccggtggg gggacaccca ggtccggccc atggccagcg cccagaccat
     1681 gctggcccgg agcacccgtt ggtgggccga gccgaggtcc acgagcatga cgtcgcagcc
     1741 gtcctggatg gcgccttggg cggcggccgc cgttgtcacg gggacaggac gagcagcgcc
     1801 gtcccaggtg gcgagcgcct cgaccccggt gaacaccggc atcgcgcgct ccccgtcagg
     1861 ggcggtcaag gtcaccacgg ccaggtccgc gcgctggtcg cccacctgac cctgaggacc
     1921 ctccgcggct tcgccgggga ccgccacgac ggggacgagg agccggctcg gggcgagaac
     1981 cgccatgagg gcctcctcgt cgccatcgcc cagcgcggcc aggaccgccg ggtcactgcg
     2041 gccccggtcg ccgccgaagc ccccgccggt cagctcacga gcctgccagg gcacgcccgc
     2101 cgagtcccca tggtggtcgt ggtcgtggtc gcagctcatg acaaccgctc tcgatcgccg
     2161 gcagcaccgg tcgccgggag cacggcgtcg acctcgatct cgacgcgcat cgcggggtcg
     2221 acgagcccgg cgacgaggac catcgtcgcc gcgggacgca cctgtccgaa ccagcgggcg
     2281 tgagcctcac cgaccgcgcc gaagtcgccg cggtgcacga catacatgcg ggtccggatg
     2341 atgtcctcga gggtgccgcc gagtgcctcg accgcgctgg tcgcggtctg cagcgccacc
     2401 tccgcctgtg ccccggcatc atcgggatgc gccacggtcc cgtcgacgac ggaggtgcac
     2461 ccggagaccg cgaccagccc acccgagcgc accgcgcggg agtagccgta ccgctgctcc
     2521 caagggccac cgctttcgac ggaccgtcgg ctcatggccg tcccgcgagg tcgagcgctt
     2581 ccggcagggt gaacgccccc cggtagagcg ccgacccgat gatggccccc tcgaccccct
     2641 ctgggacgag cgcgcggatg attcggatgt cctccagtgt cgagacccca cccgacgcga
     2701 ccaccgggcg gtcggtgcgt gcgcacacgt cccgcagcaa ggcgaggttg ggtcccgcca
     2761 gcatgccgtc cttgtcgacg tcggtgacga cgtagcgggc gcagccctcg gagtcgagcc
     2821 gggccagggt ctcccacacg tcaccgccct ccgtggtcca gccccgcgcg gcgagtgtcg
     2881 tcccccgcac gtcgagcccg accgcgaccc ggtcgccgtg ctcggcgatg acctgtgcgg
     2941 tccactcggg gtgctcgagc gcagccgtgc cgaggttgac ccgtcggcac ccggtcgcca
     3001 gggcggtggc gagggacccg gcgtcccgga tccctccgga gagctcgacc tgcaggtcca
     3061 ggcgcccgac gatcgaggcg atgagctcgc cgttggagcc ccgtccgaac gcggcgtcga
     3121 ggtcgacgag gtggatccac tcggcaccct ggtcctgcca ggccttggcc gcctcccacg
     3181 ggtcgccgaa ctggccgccg ctgccggcaa caccctggac cagctggacg gcgactcccc
     3241 cggcgatgtc gacagccggg agcagctcca ggcgggggtc ggcggtcacg aggtcgttcg
     3301 gtgtcatgtc gttcggtgtc atgtcgttga gtgtcatagt gacctcaccc agttctcgag
     3361 caggtgcgcc ccagcgtccc cggacttctc cgggtggaac tgggtcgccg acagcggtcc
     3421 gtcctcgacg gccgcgacga agggttcgcc gtgctcgctc cacgtcacga tgggggccac
     3481 cgcggcgaag ccaccctcgg ggtgcggggg ccactcggtc acggcatacg agtggacgaa
     3541 gtagaagcgc tcgccctcga caccggtgaa cagccgcgag ccctcgccgg gacacacggt
     3601 cgaccagccc atatgcggca cgacgtcggc ctggagccgg cggactatgc cggcccactc
     3661 gccgagcccc tcctgcgggc cgcgggcata cggctcggtg gagccctcga agagcacctg
     3721 catcccgacg cacaccccga gcaccggacg gccaccagcc aggcggacgt cgacgagccg
     3781 ggcgccgtcc accgcccgca ggccgagaat gcaggcatgg aagttgccga ctccggggac
     3841 gaccagaccg tcggccgcga gcgccagggc gtggtcggcg ctcagcgtca cctcggcgcc
     3901 gacgcgagcg agtgcgcgca cggccgagcg gacgttgccg ctgccgtagt cgagcacgac
     3961 gacccgcctg gtcactgttg ctcctccacc tcttcgcgcc acgagcgttc ttcacgggcg
     4021 acggtctcga agcctgccac ccgccggtcc gacggatcga cccggaccac cccccggacg
     4081 ctggtcaccg tcgtgctgcc ctcgaacagc gcaccacctg gcagcccgag cacggtgagg
     4141 acgtcgacca ggacactgcg cgcgtccccg gcccggtcgg tgagcacgtc cttgaggtcc
     4201 tcgcgagtgc ccgggaccgt ggcgccgctg gccgagacga tcgccgacag cggggccagc
     4261 ggcaggtgtg ccggacgggt gagccccacc cctggcgtcc acgcggccca gcgccggatc
     4321 gcgcgccacc ggcgcgggga cacggccacg agccccacct ctgcggcgac ggccaccccg
     4381 atggccgggc ggagccgggt gggcacgtca cggttgaaga gcatggccac ggcgtcgtgg
     4441 tagggcggcg cgacagccga gatgagcccg accgggagca ccccggtcca gccctgaccg
     4501 agcgggacga cgtcgacccc cacctgcccg cggtaggccc aggccgccac ggcgttggga
     4561 gacccgcgca cgagcagcag acccgcaggt ctgcccgggt cggtcatcgg ctcgcccttc
     4621 tcgtcggcgc ctcacggtca ccgggctaga gcgtgccctt ggcgctcggt atgccggaca
     4681 cccgcgggtc gcgcgcgacg gcagcacgca gcgcccgcgc cacagccttg aactgggcct
     4741 cgacaatgtg atgcgggtcg cgaccggcca gcacccggac gtgcagggcc agaccaccgt
     4801 ggtgagccag gctctcgaac acgtggcggg tcagcgaccc ggcatacgag ccaccgatga
     4861 ggacgtacgc ctggccctcc ggctcacccg agtgcacgac atacggccgt ccggcgacgt
     4921 cgacgacggc ctggaccaga gcctcgtcga gcgggacggt cgcgtcgccg aagcgggaga
     4981 tgccgctctt gtcaccgagc gcctcccgca gtgcctggcc gagcgcgatc gcgacgtcct
     5041 cgacggtgtg gtgcgcatcg acgtcgatgt ccccggtggc ccggatgtgc aggtcgatca
     5101 gcgcgtgctt ggccagggac tcgagcatgt ggtcgtagaa ccgcacgccc gtcgacacgt
     5161 cggactcgcc ggtgccgtcg aggtcgaccc ggatctcgac cgaggactcg ctcgtcgcgc
     5221 ggctcacggt ggccgtccgg tgtgtcatcg cgtctcctgc gagtcggtgg ctccccgccg
     5281 gtgggtggga gcgagcgcgg ccatgacatc gaggaacgcg gaggtctcct gcggcgtgcc
     5341 ggccgtgact cgaagatagt gggcaatgcc gacgtcgcga accagcacac ccccgtcgag
     5401 cagcgcgcgc cacgtcgccc gctcgtcgcg cagtccgccg aagaggacga agttcgcatc
     5461 actgggcacg acatcgagcc ccatcgcgac gagctccgtg accatccggt cgcgttgcgc
     5521 cgtgatcgcc tcgaccgtcg acaggagcga gcccgcatgc gccagcgccg cccgggccac
     5581 cgcctgagtg gggtcggaca ggtggtaggg cagccggacg aggcgcagcg cgtcgacgag
     5641 ctcggggtcg gccgcgaggt agccgagccg ggccccggcc agcgcgaagg ccttgctcat
     5701 cgtccgggtg accgcgagca ggggatgccc gaacaacagg gtcagggcgc tcgccgtgcc
     5761 ggggcgcgcg aactcggcat ccgcctcgtc gacgacgacg agcgagcccg gagccgcatc
     5821 gaggaccgcc tcgacgacat cgagcgagag cgctgtcccg gtcgggttgt tcggtgagca
     5881 gaggaagacc acctggggcc ggacctcgcg gacctgggct acgaccgttg ccgggtcgag
     5941 ctcgaaggcc gcgccgggcg acgggccggc cacaccccgg atgccgtcga cccagcgggt
     6001 ccccagcgtc tcggcgatga tcgggtgcat cgagtaggcc ggcgtgaacc cgagcgccgt
     6061 gcgccccgga cccccgaagg cctggacgag gtgctgcagg acctcgttgg acccgttgcc
     6121 ggcccagatc ccggccgggt cgatttcgac tccggactgg acccgcaggt atgccgcgag
     6181 ctcggtccgc agcgcggtga actcgcggtc ggggtaccgg ttcagccccg cggcggctac
     6241 cgcgaccgcc gcggtgatcg ccgcggccac cggcccgggc acgggatagg agttctcgtt
     6301 ggtgttgagg cgcaccggca cgtcgagctg cggcgcgcca tacgccgtcc tgccgcgcag
     6361 ctcgggccgc agcaggtccg cgatggcgct caccgcgcgg ccgcgtccgg cagcgcgtcc
     6421 ccgaaccggg ccaggacggc ctccccgtgg gcgggcaggt cctcggactg ggcgagggtg
     6481 aggaccgtgc gggcgatctc gcgcagcgcc ggctcgtcgt aggccacgac gtggacgccc
     6541 cgcaggaacg actgcaccga cagaccgctg ctgtggcagg cgcagcccgc ggtgggcagg
     6601 acgtggttgg agcccgcggc gtagtcgccg aggctgactg gggcgtaggc gccgacgaag
     6661 acggcaccgg cgttgcggac ctgggcggcg accgcctcgg cgtcgcgggt ctggatctcg
     6721 aggtgctcgg cggcgtaggc gttgcagacg gcgagcccgg cgctgacgtc gtcgacgagg
     6781 accaccctgg actgacggcc ggccagcgcg gtggccacgc gctcgctgtg cttggtggcc
     6841 gggacacgcc gggccagtgc cgcctcgacc gcgtcggcca gctcctcgct gtcggtgacg
     6901 agcacggcgg cggccagcgg gtcgtgttcg gcctgcgaga tgaggtcggc ggcgacatgc
     6961 tcggcgtcgg cgctgtcgtc ggcgaggacg gcgatctcgg tgg
//
LOCUS       DATAIX010000556         1870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_251427, whole genome shotgun sequence.
ACCESSION   DATAIX010000556 DATAIX010000000
VERSION     DATAIX010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251427"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VHM65_08275"
     CDS             complement(<1..261)
                     /locus_tag="VHM65_08275"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEX2705741.1"
                     /translation="MCGRYAASARNADLVEEFGIEEDQTGVPSRSILVNPQEPPAGEP
                     DFNMAPSKQARVVVTRVSRAGTAHGGSAAPEPTRRLRLLTWGL"
     gene            366..1742
                     /gene="aroA"
                     /locus_tag="VHM65_08280"
     CDS             366..1742
                     /gene="aroA"
                     /locus_tag="VHM65_08280"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX2705742.1"
                     /translation="MTDQPPRDWAAPRVRGPLHATVRLPGSKSLTNRFLVLAALANDR
                     SVIRRPLRSRDTLLMADALRSLGVLIEDVGAAGARPALKAGAGPVLKAGPGARAPRGT
                     LADWLVTPPQTLRGGVGIDVGLAGTVMRFLPPVAALADGPVDFDGDPESRLRPMGPLL
                     GALRELGVKVADQSRNALPFTVAGTGRVTGGVATFDASASSQFVSALLLAGARYDEGV
                     TVRHRGRPVPSEPHIAMTVETLRDVGVIVDDDDANLWRVEPSEINALDVVVEPDLSSA
                     APFVAAALVAGGTVAIPGWPRHTTQGGDALRQLLSDMGADVRLSRSGLTVTGTGAIAG
                     IDIDLHEASELTPVVAALAALADTPSRIRGVAHIRGHETDRIRALSTEINRLGGKVSE
                     TEDGLRIQPARLKGRRFRTYHDHRMAMAGAVLGLRVRDLVVEDIATTAKTLPGFTELW
                     MAMLDGTG"
BASE COUNT          249 a          699 c          666 g          256 t
ORIGIN      
        1 gagtccccag gtgagcagcc ggagccggcg cgtgggctcg ggcgcggcgc tgcccccgtg
       61 ggctgtcccg gcacgggaca cccgggtcac caccacgcgc gcctgcttgc tcggggccat
      121 gttgaagtcg ggctcccccg caggtggctc ctgggggttg accaggatgc tgcgtgacgg
      181 gacgcccgtc tggtcctcct cgatgccgaa ctcctcgacg aggtcagcat tgcgggcact
      241 ggccgcatac cgtccgcaca tgccgccagc gtagggcgag caccacggtc ggcgggcccc
      301 tcgccctccc cgcgaccgcg gtcgcctcat cccgggcggt ctcggatagg atctgcaccg
      361 cgacgatgac agaccaacct ccccgagact gggcggcacc acgggtacgc gggccgctcc
      421 atgcgactgt gcggctgccc gggagcaagt cgctcacgaa ccggttcctc gtcctggctg
      481 ccctcgccaa cgaccggtcg gtcatccgcc ggccgttgag gtcgcgcgac accctgctca
      541 tggccgacgc gctgcgctcg ctcggggtcc tgatcgagga cgtcggagcg gccggggcca
      601 ggccggccct caaggccggg gccgggccgg tcctcaaggc cggcccggga gcccgggccc
      661 ccaggggcac gctcgcggac tggctcgtga cccctccgca gaccctgcgt ggcggcgtgg
      721 ggatcgacgt cggcctggcc gggaccgtca tgcggttcct gcctcccgtt gcggcgctcg
      781 ccgacgggcc ggtggacttc gacggggacc ccgagtctcg cctccgcccg atgggcccac
      841 tgctgggagc cctgcgtgag ctgggcgtca aggtcgcgga ccagagccgc aacgcgctgc
      901 cgttcacggt cgcgggcacg ggccgggtca cgggcggcgt tgcgacgttc gacgcgtcgg
      961 caagctcgca gttcgtctcg gcgctgctcc tcgccggagc ccggtatgac gagggggtga
     1021 cggtccgcca ccgtggcaga cccgtgccga gcgagccgca catcgcgatg acggtggaga
     1081 ccctgcgcga tgtcggcgtc atcgtcgatg acgacgacgc caacttgtgg cgggtcgagc
     1141 ccagcgagat caacgccctc gacgtcgtgg tcgagcccga cctgtccagc gccgctccgt
     1201 tcgtcgcggc ggcgctcgtc gcgggaggca ccgtggccat ccccggatgg ccacgccata
     1261 ccactcaggg cggcgatgcg ctgcgccagc tcctctctga catgggcgcc gacgtgcggc
     1321 tgtcccggtc cgggctcacc gtcaccggca ccggcgccat cgccgggatc gacatcgacc
     1381 tccacgaggc gagcgagctg acccccgtcg tggcggcgct cgcggccctc gcggacaccc
     1441 cttcgcggat ccgcggggtc gcccacatcc ggggccacga gaccgaccgc atccgtgccc
     1501 tctcgaccga gatcaaccgg ctcggtggca aggtcagcga gaccgaggac gggctccgga
     1561 tccagccggc tcgcctcaag ggcaggcgtt ttcgcaccta ccacgaccac cgcatggcca
     1621 tggccggagc agtgctcgga ctgcgtgtgc gcgacctcgt cgtcgaggac atcgccacga
     1681 ccgccaagac cctgcccggc ttcaccgagc tctggatggc gatgctggac ggcaccgggt
     1741 gagctggcgc gacctcgacg agtcggacgt ccgggtccgc cccaaccgcc ggggcagccg
     1801 cccgcggtcc aaggaccgac cgttgcacga ggacgccatc gagggagtgg tcaccggggt
     1861 cgaccggggc
//
LOCUS       DATAIX010000557         2984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_251486, whole genome shotgun sequence.
ACCESSION   DATAIX010000557 DATAIX010000000
VERSION     DATAIX010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251486"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VHM65_08285"
     CDS             <1..281
                     /locus_tag="VHM65_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705743.1"
                     /translation="AGVKGVSEQSLPLLSEVTNTVSATNEQIARVDTITTNVATMSTN
                     VNALTSLFAATLGTPVVKVAAFSYGVRSALRVASAPRASRRARHGTGR"
     gene            287..532
                     /locus_tag="VHM65_08290"
     CDS             287..532
                     /locus_tag="VHM65_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705744.1"
                     /translation="MKRLLWVGLGVGVAAGAAVLVIRYGDRLGELARVVRDGMTEREA
                     ELRAALGLDTGLSEGHGELDAAAARRLLQDPTGPRAR"
     gene            539..>2984
                     /gene="alaS"
                     /locus_tag="VHM65_08295"
     CDS             539..>2984
                     /gene="alaS"
                     /locus_tag="VHM65_08295"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX2705745.1"
                     /translation="MDTADIRSRWLAFFEGRGHTVVPSAPLPYEDPNLLFVNAGMVPF
                     KPYFLGQESAPYRRATSVQKCVRTLDIEEVGKTTRHGTFFQMNGNFSFGDYFKEGAIE
                     LAWELVTRPRSDGGYGFAERDLFASVYHEDSEAIDLWRRVAGLPEERIIRLGKKDNYW
                     SMGVPGPGGPCSEILIDRGPAFGAEHDWDAGDRYLEFWNLVFMQDALSAVRSKEDFDI
                     EGPLPMKNIDTGMGLERVAFLLQGVDNMYEIDEIFPVIAAAAEQSGRRYGANHEDDVR
                     FRVIADHVRSALMLIGDGVTPGNEGRGYVLRRLMRRAIRSMRLLGVHDPTLPVLLPVS
                     KAQMAKSYPELDTAWERISQVAYAEEDAFRRTLTSGTALFDTAVARVVSTGSGTLAGD
                     DAFALHDTYGFPIDLTLEMAAEQGVHVDEDGFRRLMSEQRERAKADARAKRSVHGDTG
                     SYRRLADALGRDVEFTGYDEVVSEAHVHGLLRDGEAVQAVSTGEDIELVLDRTPFYAE
                     GGGQLADGGRIQLASGAVVKVHDVQKPIGGLIVHRATVLVGEVGVGDEAQAEVDIERR
                     RAISRAHTATHMVHKAVREALGETATQAGSENAPGRFRFDFSAQSAVPPSVLHDVEAR
                     VNAILLDDLSVHADVMTQEQARGIGAMALFGEKYGDAVRVISVGDWARELCGGTHAQR
                     TGQLGVVKLLGESSIGAGVRRVEALVGADAYSFLAREHAIVGQLSEVLKARPEELPER
                     IAGILARLHDAEKEIAAMRRHQALAGAAALVSGASDVGGVTVLAHDAGEVAADDLRTL
                     VLDIRRRLGAERPSVAA"
BASE COUNT          445 a         1062 c         1059 g          418 t
ORIGIN      
        1 gggcgggcgt caagggcgtc tccgagcaga gcctgcccct gctttctgag gtgaccaaca
       61 ccgtgtcggc caccaacgag cagatcgccc gggtcgacac catcacgacc aacgtcgcca
      121 cgatgtcgac gaacgtcaac gccctcacct cgctcttcgc ggcgaccctc gggaccccgg
      181 tcgtcaaggt cgccgcgttc agctacggcg tgcgcagcgc actcagggtg gcaagcgcgc
      241 cccgggccag ccggcgcgcc cgtcacggca ccgggcgctg agccccgtga agcgcctgct
      301 ctgggtcggc ctcggcgttg gcgtcgccgc gggagccgcg gtgctcgtca tacggtatgg
      361 cgaccgcctg ggtgagctgg cccgggtggt ccgcgacggc atgaccgagc gcgaggccga
      421 gctgcgcgcg gccctcggcc tcgacacggg cctgtccgaa ggccacggcg agctcgacgc
      481 ggctgccgcg cgccgcctgt tgcaggaccc caccgggccg cgagcccgtt agcacatcat
      541 ggacaccgca gacatccgca gccgctggct cgccttcttc gagggccgcg gccataccgt
      601 cgtcccgtcg gcaccgctgc cctacgagga ccccaacctg ctcttcgtca acgccgggat
      661 ggtgccgttc aagccgtact tcctcggcca ggagtccgcg ccctaccggc gggcgacgag
      721 cgtgcagaag tgcgtgcgga ccctcgacat cgaggaggtc ggcaagacca cccgccacgg
      781 gaccttcttc cagatgaacg gcaacttctc cttcggggac tacttcaagg agggggcgat
      841 cgagctcgcc tgggagctgg tcacccgccc gcgcagcgac ggcggctacg gtttcgccga
      901 gagggacctc ttcgcctcgg tctaccacga ggactccgag gccatcgacc tgtggcgccg
      961 ggtcgcggga ctgccggagg agcggatcat ccgcctgggt aagaaggaca actactggtc
     1021 gatgggcgtg cccgggccgg gtggcccgtg tagcgagatc ctcatcgacc gcggaccggc
     1081 cttcggcgcc gagcacgact gggatgcggg ggaccgctac ctcgagttct ggaacctcgt
     1141 cttcatgcag gacgccctct cggccgtgcg cagcaaggag gacttcgaca tcgaaggccc
     1201 cctgccgatg aagaacatcg acaccggcat gggtctggag cgggtcgcct tcctgctcca
     1261 gggggtcgac aacatgtacg agatcgacga gatcttcccg gtgatcgccg ccgccgcgga
     1321 gcagtccgga cgccgctatg gcgcgaacca cgaggacgac gtccgcttcc gggtcatcgc
     1381 cgaccacgtc cgctcggcgc tcatgctcat cggtgacggg gtgacgcccg gcaacgaggg
     1441 gcgaggctac gtgctgcgcc ggctcatgcg ccgggccatc cgctcgatgc gcctgctcgg
     1501 cgtccacgac ccgacgctgc ccgtcctgct cccggtgagc aaggcccaga tggccaagtc
     1561 ctaccccgag ctggacacgg cgtgggagcg gatcagccag gtcgcgtatg ccgaggagga
     1621 tgccttccgg cgcaccctca cctccgggac ggccctgttc gacacggcgg tcgcacgggt
     1681 cgtgagcaca gggtcgggca cgctggccgg cgacgacgcg ttcgcgctgc acgacaccta
     1741 cggcttcccg atcgacctca ccctcgagat ggcggccgag cagggggtgc acgtcgacga
     1801 ggacggcttc cggcggctca tgagcgagca gcgcgagcgc gccaaggccg acgcgcgggc
     1861 caagaggagc gtccacggtg acaccggcag ctaccgccgg ctcgccgatg ccctggggcg
     1921 cgacgtcgag ttcaccgggt atgacgaggt cgtctccgag gcccacgtcc acggtctgct
     1981 gcgcgacggg gaggcggtcc aggccgtctc caccggtgag gacatcgagc tcgttctcga
     2041 ccggacgccg ttctatgccg agggaggtgg ccagctcgcc gacggtgggc ggatccagct
     2101 cgccagcggc gccgtcgtca aggtccacga tgtccagaag cccattggcg gtctcatcgt
     2161 ccaccgggcg acggtgctcg tgggcgaggt cggcgtcggc gacgaggcgc aggccgaggt
     2221 cgacatcgaa cggcgccggg ccatctcgcg cgcacacacg gcaactcaca tggtgcacaa
     2281 ggcagtccgt gaggcgctcg gcgagacggc gactcaggcc gggtcggaga acgcacccgg
     2341 ccggttccgg ttcgacttca gcgcacagtc ggccgtgccg ccgtcggtcc tgcacgacgt
     2401 cgaggcgcgg gtcaacgcca tcctgctcga cgacctgtcc gtccacgccg acgtcatgac
     2461 gcaggagcag gcccgcggca tcggggccat ggcgctgttc ggcgagaagt acggcgacgc
     2521 ggtgcgggtc atctcggtcg gcgactgggc gcgtgagctg tgcgggggca cccacgccca
     2581 gcgcacgggt cagctcgggg tcgtcaagct gctcggggag tcctcgatcg gggccggggt
     2641 gcgccgggtc gaggcgctgg tcggcgccga tgcctactcc ttcctcgccc gcgagcacgc
     2701 catcgtgggt cagctcagcg aggtcctcaa ggcccgcccg gaggagctgc ccgaacgcat
     2761 cgccgggatc ctcgcgcggt tgcacgacgc cgagaaggag atcgccgcga tgcgccggca
     2821 ccaggcgctc gcgggcgcgg cggcactggt gtcgggcgcg tccgatgtcg ggggagtcac
     2881 cgtcctcgcc catgacgccg gcgaggtcgc ggccgacgac ctgcgcaccc tcgtcctcga
     2941 catccgccgg cgcctcggtg ccgagcggcc gtccgtggcc gcac
//
LOCUS       DATAIX010000558         3771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_281470, whole genome shotgun sequence.
ACCESSION   DATAIX010000558 DATAIX010000000
VERSION     DATAIX010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281470"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..507)
                     /locus_tag="VHM65_08300"
     CDS             complement(250..507)
                     /locus_tag="VHM65_08300"
                     /inference="COORDINATES: protein motif:HMM:NF022832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HEX2705746.1"
                     /translation="MSGHESAVVRMGHDIVRQLAHLPRALAAEEIATHIKKFWEPRMR
                     HELLARIRSGDMTLEPLLVAAAEHLVDGETDDAEMAEPSGG"
     gene            complement(504..3377)
                     /gene="fdhF"
                     /locus_tag="VHM65_08305"
     CDS             complement(504..3377)
                     /gene="fdhF"
                     /locus_tag="VHM65_08305"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX2705747.1"
                     /translation="MTLIAEPDYGTPAVAADSTVCVTVDGCTVEVPAGTSVLRAASLA
                     GVDVPRLCATDSLKAFGSCRLCLVEVDGMRGTPASCTTPCTDAMVVSTQTEQVRDLRR
                     GVLELYLSDHPADCAGCARGSCEMQDLAHRVGVAEVRYGLGGGAGGKHHLGATRDVSN
                     PYFDYDPSACIVCSRCVRACDEVQGTFALTVEGRGFDSRISPGGAAGFMASECVSCGA
                     CVQACPTSALEEKSLVALGMPTRSVMTTCAYCGVGCSFKAEVRDEPSAEGVLTRVVRM
                     MPAKSGGANEGHSCVKGRFAYGYAAHRDRQLAPMVRDSIEDQWRVVSWDEAIARAASG
                     FQAIQARYGVASIGGISSSRCTNEEVYVVQKMVRAAFGNNNIDTCARVCHSPTGYGLS
                     QTFGTSAGTQDFRSVEQADVVLLIGANPTDAHPVFASRLKRRLRAGARLIVADPRRIE
                     LVRSPHVEATHHLRLLPGTNVAFVNAMAHVILTEGLADHPFIEARCEDFASYEAFIAD
                     PGNSPEALEVTTGVQASEIRSAARLFATGGNAAIYYGLGVTEHSQGSTMVMGMANLAM
                     LTGNIGRPGVGVNPLRGQNNVQGSCDMGSFPHELPGYRHISLDDVRGIFERLWGVLIP
                     ATPGLRIPNMFDSALGGSFRGLFVQGEDIAQSDPNTTHVEASLRAMDLVVVQDLFLNE
                     TARFAHVFLPGTSFLEKDGTFTNAERRINRVRPVMAPAQGRDEWQIVCDLAAAMGYPM
                     SYGSSAEIMDEIAATTPTFAGVSFARLDALGSLQWPVNDRAPEGTPIMHVGGFVRGKG
                     RFIRTVYVPTTEQTTRRYPLILTTGRILTQYNVGSQTRRTDNSAWHPEDVLEIHPTDA
                     QLRGIAGGDLVELASRVGTTTLRALVTDRMPLGVVYTTFHHPVTGANVVTTENSDWAT
                     NCPEYKVTAVQVAVATARDVGAQAGTRHTYDIDPAEPSLAGRR"
     gene            complement(3374..>3771)
                     /locus_tag="VHM65_08310"
     CDS             complement(3374..>3771)
                     /locus_tag="VHM65_08310"
                     /inference="COORDINATES: protein motif:HMM:NF022059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX2705748.1"
                     /translation="LPADRLGLAMDYEAFAAADAMVGHGGVVVFDDTVDMAHQARFAM
                     EFCAAESCGKCTPCRVGSQRGVETIDRIIAGVQPDRNLELLADLCTTMAKGSLCAMGG
                     LTSMPVLSALRHFPEDFDHTPTEPQGAPS"
BASE COUNT          575 a         1251 c         1398 g          547 t
ORIGIN      
        1 accacgcgat cagcgcagcc acgcaccgcg ccggtcgagc gcggggcggg ttcatggaca
       61 cggccggata cgccgagtgc acgtgcgaga ccgcgtgctg gctcggcacg acctgcgagc
      121 gggactgctc ctgccagtgc gagccggctg cgtgggagcc cacggcctga cctagcggtg
      181 agaagagatg gggcggtctg gcatcgagca ccggcaccat gcccggcccg cggcgggatc
      241 gggtcctcgt cagccgccgc tcggctcggc catctcggcg tcgtccgtct cgccgtcgac
      301 gaggtgctcg gcggcggcga cgagcagagg ttcgagggtc atgtcgccgg accggatgcg
      361 cgccaagagc tcgtggcgca tccgcggctc ccagaacttc ttgatgtggg tcgcgatctc
      421 ctccgcggcg agcgcacggg gcaggtgtgc gagctggcgc acgatgtcgt gccccatccg
      481 caccacggca gactcatgcc cgctcatcgc cggccagcga ggctcggctc ggccgggtcg
      541 atgtcatagg tatgacgggt gcccgcctgc gcgccgacgt cccgggccgt ggcgacggcg
      601 acctgcacgg cggtgacctt gtactccggg cagttcgtcg cccagtcgga gttctcggtc
      661 gtgacgacgt tggccccggt gacgggatgg tggaaggtcg tgtagacgac tcctaggggc
      721 atgcggtcgg tgaccaaggc ccgcagggtg gtcgtcccga cgcgagaggc cagctcgacg
      781 aggtcgccgc cggcgatgcc gcgcagctgg gcgtcggtcg gatggatctc gagcacgtcc
      841 tcggggtgcc aggccgagtt gtcggtgcgg cgggtctgcg agccgacgtt gtactgcgtg
      901 aggatacgcc cggtcgtgag gatgagcggg tagcgccggg tggtctgctc ggtcgtcggc
      961 acgtacacgg tccggatgaa ccggcccttg ccgcggacga acccgccgac gtgcatgatc
     1021 ggcgtccctt cgggggcgcg gtcgttgacc ggccactgca gcgagcccag ggcgtcgagc
     1081 cgggcgaagg acacgccggc gaacgtcggt gtcgtggccg cgatctcgtc catgatctcg
     1141 gccgacgacc cgtaggacat ggggtagccc atggcggcgg cgaggtcgca gacgatctgc
     1201 cactcgtccc ggccctgcgc aggggccatg accgggcgga cacggttgat ccggcgctcg
     1261 gcgttggtga acgtcccgtc cttctcgagg aacgaggtcc ccgggaggaa cacgtgggcg
     1321 aaccgcgccg tctcgttgag gaagaggtcc tggacgacga cgaggtccat cgcgcgcagc
     1381 gaggcctcga cgtgggtggt gttggggtcg gactgggcaa tgtcctcgcc ctggacgaag
     1441 agcccgcgga acgacccgcc gagagccgag tcgaacatgt tggggatccg cagccccggc
     1501 gtcgccggga tcagcacgcc ccagagccgc tcgaagatgc cccggacgtc gtcaagggag
     1561 atgtgccggt agcccggcag ctcgtgcggg aaggagccca tgtcacacga gccctggacg
     1621 ttgttctgcc ctcgcagcgg gttgaccccg acaccgggcc ggccgatgtt gccggtcagc
     1681 atcgcgaggt tggccatgcc catcaccatc gtcgagcctt ggctgtgctc ggtgacaccc
     1741 aggccgtagt agatcgccgc gttgccacct gtcgcgaaga gccttgctgc tgaacggatc
     1801 tcgctggcct ggacacccgt ggtcacctcg agcgcctcgg gcgagttccc agggtcggcg
     1861 atgaacgcct cgtatgacgc gaagtcctcg caccgcgcct cgatgaacgg gtggtcggcc
     1921 agtccttcgg tgaggatcac atgcgccatg gcgttgacga aggcgacgtt ggtgcccggc
     1981 agcagccgca gatggtgggt ggcctccacg tgcggggacc ggaccagctc gatccggcgt
     2041 gggtcggcca cgatgagccg ggcgccggca cgcagccgcc gcttgagccg ggaggcgaac
     2101 acgggatggg cgtcggtcgg gttggccccg atgagcagga cgacatcggc ctgctcgacc
     2161 gacctgaagt cctgcgtgcc ggcggacgtg ccgaacgtct ggctcaggcc atagcccgtg
     2221 ggggagtggc aaacccgggc acaggtgtcg atgttgttgt tgccgaaggc cgcacggacc
     2281 atcttctgga cgacgtacac ctcttcgttc gtgcaccgcg aggaggagat cccgccgatg
     2341 gaggcgacgc cataccgcgc ctggatcgcc tggaatccgg aggcggctcg cgcgatggcc
     2401 tcgtcccacg agacgacgcg ccactggtcc tcgatcgagt cccggaccat gggggcgagc
     2461 tggcggtcac ggtgggcggc atagccgtag gcgaagcggc ccttgacgca ggagtggccc
     2521 tcgttggcgc cgccggactt ggccggcatc atccggacca cgcgggtgag gacaccctcg
     2581 gcactgggct cgtcacggac ctcggccttg aacgagcagc cgaccccgca gtaggcgcac
     2641 gtcgtcatga ccgagcgcgt cggcatcccc agcgcgacga gcgacttctc ctcgagtgcc
     2701 gaggtggggc aggcctggac gcaggcaccg caggagacgc actccgaggc catgaagccc
     2761 gccgcgccgc cgggggagat ccgcgagtcg aagcctcgcc cctcgacggt cagcgcgaac
     2821 gtcccctgga cctcgtcgca ggcacggacg caccgcgagc agacgatgca cgcggacggg
     2881 tcgtagtcga agtacgggtt ggagacgtcc cgtgtcgcgc cgaggtggtg cttgccaccg
     2941 gcaccgccgc cgaggccata gcgcacctcg gccacaccga cccggtgggc gaggtcctgc
     3001 atctcgcagc tgccgcgcgc gcacccggcg cagtcggccg ggtggtccga gaggtagagc
     3061 tccaggacgc cacggcgcag gtcgcggacc tgctcggtct gggtagagac gaccatcgcg
     3121 tccgtgcacg gggtcgtgca cgaggccgga gtcccacgca tcccgtcgac ctcgacgagg
     3181 cacagccggc aggagccgaa cgccttgagc gagtcggtgg cgcacagcct gggcacatcg
     3241 accccggcga gcgaggccgc gcgcaggacg gaggtgccgg ctgggacctc gacggtgcat
     3301 ccgtcgacgg tgacgcagac ggtggagtcc gcggcgacgg cgggagtgcc atagtcgggt
     3361 tcggcgatga gcgtcatgag ggggcccctt gtggctcggt gggggtgtgg tcgaagtcct
     3421 cggggaagtg gcgcagggcg ctgagcacgg gcatcgacgt cagtccgccc atggcgcaga
     3481 gcgagccttt ggccatcgtc gtgcacaggt ccgccagcag ctcgaggttg cggtccggct
     3541 gcaccccggc gatgatccgg tcaatggtct cgacgccccg ctgtgacccg acccggcacg
     3601 gcgtgcactt gccgcacgac tccgcggcgc agaactccat cgcaaaccgg gcctgatgcg
     3661 ccatgtccac ggtgtcgtcg aacacgacga cgccaccgtg accgaccatc gcgtcagccg
     3721 cggcgaacgc ctcgtagtcc atggccaggc cgagccggtc ggcgggcaga t
//
LOCUS       DATAIX010000559         1561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_132222, whole genome shotgun sequence.
ACCESSION   DATAIX010000559 DATAIX010000000
VERSION     DATAIX010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132222"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1477
                     /locus_tag="VHM65_08315"
     CDS             <1..1477
                     /locus_tag="VHM65_08315"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705749.1"
                     /translation="YTGGAWGTRDVSQGPVELLLGLGRLEPLPVLLALVFRAQSTVGD
                     WPQAFGFYDDDQDFRLDPSHGDVLFWPVLALGQYLVAAGDRAVLDEQVPFYPDAGEPE
                     QASLLEHALRALALGRERVVAGTHLTAYGHGDWNDSLQPADPSMRERLCSSWTVTLHI
                     QTLRTLAAGLASVGRGEHVSGLREEADAILAEFQQHLIRDGVLAGYGHFAEDGTLRHL
                     IHPADRETGLTYSLLPMVHAISNDLLTPEQAARHARIIREHLVAPDGARLFDRPPAYR
                     GGPMVHFQRAETATFFGREVGLMYTHAHLRYAQAMARLGDGDALLRALEQANPIGIDE
                     VVPNARLRQANCYFSSSDARFADRYEAGAEYDRVRDGRVEVDGGWRVYSSGAGIAYRL
                     VVECLLGLTRRSASLVVDPVLPRSLDGLHASLHLGGRPVTVAYSVGGHGSGPSSVVLN
                     GQEMAAVRLANRYRTGGLEIDMDEVQAVLRPDANHLDVTLP"
BASE COUNT          222 a          562 c          538 g          239 t
ORIGIN      
        1 gtacacgggc ggtgcctggg ggacgcggga cgtcagccag ggacccgtcg agctgctgct
       61 gggcctcggc cggctcgagc cactgcccgt gctgctggcg ctcgtcttcc gggcccagtc
      121 gacggtgggc gactggccac aggcgttcgg cttctacgac gacgaccagg acttccgcct
      181 cgacccctcg cacggcgacg tcctcttctg gccggtgctg gcccttgggc agtacctcgt
      241 ggcggcgggt gaccgtgcgg tcctcgacga gcaggtcccg ttctaccccg atgctgggga
      301 gccggagcag gcgagccttc tcgagcatgc gctgcgcgcg ctggcgctgg gccgggagcg
      361 agtggtggct ggaacccatc tcacggccta tggccatggc gactggaacg actcgctcca
      421 gccggcggac ccctcgatgc gcgagcgcct ctgcagctcg tggaccgtca ccttgcacat
      481 ccagacgctg cgcaccctcg ccgccgggtt ggcctcggtc ggtcgcggcg agcacgtcag
      541 tgggttgcgc gaggaagccg acgccatact ggccgagttc cagcagcacc tgatccggga
      601 cggtgtcctc gcaggctacg ggcacttcgc cgaggacggg acgcttcggc acctcatcca
      661 tccggcggac cgcgagaccg gcctcaccta cagcctgctg ccgatggtcc acgcgatcag
      721 caacgacctg ctcacccccg agcaggcggc ccgtcacgcg cggatcatcc gggagcacct
      781 cgtcgccccc gacggcgcgc ggctcttcga ccggcctccg gcctaccggg gtggccccat
      841 ggtccacttc cagcgcgcgg agacggcgac cttcttcggc cgcgaggtcg gcctcatgta
      901 cacccacgcc cacctgaggt acgcgcaggc catggcgcgc ctcggcgacg gcgatgcgct
      961 gctgagagcc ctcgagcagg ccaaccccat cggcattgac gaggtcgttc ccaacgcgcg
     1021 gctgcgccag gcgaactgct acttctccag ctccgacgcc cgtttcgccg acaggtacga
     1081 ggccggcgcc gagtacgacc gggtcaggga cgggcgcgtc gaggtcgacg ggggctggcg
     1141 ggtctactcc agcggcgccg ggatcgccta ccgtctcgtc gtcgagtgcc tcctcgggct
     1201 gacccgacga tcggcgtcgc tcgtcgtcga cccggtgctg cctcggtcgc tcgacggtct
     1261 gcacgcgtcg ctgcacctcg gcggccggcc agtgacggtg gcctactccg tgggcgggca
     1321 cggcagcggg ccgtcctcgg tggtcctcaa cggccaggag atggcggccg tgcgactggc
     1381 caaccgatat cggaccggtg gactcgagat cgacatggac gaggtgcaag ccgtgctccg
     1441 tccggacgca aaccacctcg acgtgacgct gccatagcca cactgggaca tatcgcagcc
     1501 atgggcagat gaatgggcgg agagggattc tcgcccgcat tcatgtcatg acgccccgga
     1561 g
//
LOCUS       DATAIX010000560         1880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_222052, whole genome shotgun sequence.
ACCESSION   DATAIX010000560 DATAIX010000000
VERSION     DATAIX010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222052"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VHM65_08320"
     CDS             <1..894
                     /locus_tag="VHM65_08320"
                     /inference="COORDINATES: protein motif:HMM:NF037043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705750.1"
                     /translation="AMAFAGLDLAVQAIDADPATAAVATVNLRHFPEATVRAGLAETV
                     TLPSGPGARHTGVWVDPARRVTGVADSRGRTTRVFSLDAISPSWTQVQQWAARVPAAG
                     AKLSPAFAHHAIPAGAEAEWVSWRGEVLECVVWFGSLARRPGRVASVCRRDRLPVSVA
                     EADAHGGDATLGRVSQVGPYLYEADRAVVRAGLTGALVRVLDGAELDTGVGYVSADRP
                     VEVGYARRYAVLEAMPLNVKALRTWARSRGIGRLTIKKRGVTLDPDLLRRQLRLAGEA
                     SATVVLTRVAGGQVVVVVEPR"
     gene            complement(1090..>1880)
                     /locus_tag="VHM65_08325"
     CDS             complement(1090..>1880)
                     /locus_tag="VHM65_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX2705751.1"
                     /translation="GGGFFGLGCGFGLRLGLAGQCVITQARSAAPPLLLPLPGARVTM
                     VGAVGDDEAGAAYGARLGRFGIEVRLRTVPGCPTGHAYITVDAAHENSIVVAPGANGR
                     LHVGDLDPLDTMDADDILLCQLEVPLDTVASAVRRAAHRGARVVLNCAPFASLPADVV
                     LLADPLVCNEHEARQVADAGMLPASWLVTFGAAGAAWDGEQQTAPHVGAQDLQDTTGA
                     GDAFCGALAAALARRASRPTAMGEALAAGAEAVTRMGAQRDPML"
BASE COUNT          242 a          719 c          679 g          240 t
ORIGIN      
        1 gccatggcgt tcgccgggct cgacctcgcc gtccaggcca tcgacgccga cccggccacc
       61 gccgcggtcg ccaccgtcaa cctgcgccac ttccccgagg ccaccgtccg ggccggcctc
      121 gccgagacgg tgaccttgcc cagcggaccc ggggcgcgcc ataccggagt ctgggtcgac
      181 cccgcgaggc gcgtcacggg agtcgcggac tcgcgggggc gcaccacccg agtcttctcc
      241 ctcgacgcga tctcgccgtc ctggacccag gtccagcagt gggccgcccg ggtccccgcg
      301 gcgggagcca agctgagccc cgccttcgcg caccacgcga tcccggccgg cgccgaggcg
      361 gagtgggtct catggcgggg cgaggtcctc gagtgcgtgg tgtggttcgg ctcgctcgca
      421 cgccggcccg gccgggtggc ctcggtatgc cgccgcgacc ggctgccggt cagcgtcgcc
      481 gaggccgacg cgcacggcgg cgatgccacg ctcggccggg tgagccaggt cgggccctac
      541 ctctacgaag ccgaccgtgc cgtcgtacgc gccgggctca ccggtgccct ggtgcgcgtc
      601 ctcgacggcg cggagctgga caccggggtc ggctacgtca gcgcggaccg gccggtcgag
      661 gtcggctacg cgcgccggta tgccgtgctc gaggccatgc cgctcaacgt caaggcgctg
      721 cgcacctggg cgcgctcgcg cgggatcggg cggctcacca tcaagaaacg gggcgtgacc
      781 ctcgacccgg acctcctgcg ccgccagctg cgcctcgcgg gtgaggcgtc ggcaacggtc
      841 gtcctcaccc gcgtcgcggg cggtcaggtc gtcgtcgtgg tcgaaccccg ctgagagcgc
      901 gtctcgcgac tctggtgctg gccgggtgcg cgttggttgt ggcccgtggt gagcaggtga
      961 gcacctggtg ggtccatggt tgtcgagacc actccacccg tggctcaccg tgcccgatcc
     1021 agccggccgg caccggccac atccacctca gcagcccaaa cgtcgcacac gcgcgctcgt
     1081 gccgcgtggt cacagcatgg ggtcgcgctg ggctcccatc cgggtcacgg cctcggctcc
     1141 cgcggccagg gcctcaccca tggccgtcgg ccggctcgct cgcctcgcga gcgccgctgc
     1201 gagcgccccg cagaaggcgt caccggcccc ggtggtgtcc tgaaggtcct gcgcgcccac
     1261 gtgcggcgcg gtctgctgct caccatccca ggcggccccg gcagcgccga aggtgacgag
     1321 ccaggacgcc gggagcatcc cggcatcggc gacctgccgc gcctcgtgct cgttgcagac
     1381 cagtgggtcg gccaggagca ccacatcggc gggcaggctc gcgaacggcg cacagttgag
     1441 cacgacccgc gcccccctgt gggcggcacg gcggaccgcg gacgccacgg tgtccagcgg
     1501 gacctccagc tgacagagca ggatgtcatc ggcgtccatc gtgtcgagcg ggtcgaggtc
     1561 gcccacgtgg agccgtccgt tggcgccggg cgccacgacg atcgagttct cgtgcgccgc
     1621 gtccaccgtg atgtaggcgt ggcccgtcgg gcagcccggc accgtgcgca gccgaacctc
     1681 gatcccgaac ctgcccaggc gtgcgccata cgctgctccg gcctcgtcgt cgcccacggc
     1741 accgaccatc gtgacccggg ccccgggcag gggcaggagc aggggcgggg ccgcggaccg
     1801 ggcctgggtg atgacgcact gaccggcgag gccaagacga aggccgaagc cgcagccaag
     1861 gccaaagaac ccaccgccac
//
LOCUS       DATAIX010000561         1829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_192660, whole genome shotgun sequence.
ACCESSION   DATAIX010000561 DATAIX010000000
VERSION     DATAIX010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192660"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VHM65_08330"
     CDS             <1..281
                     /locus_tag="VHM65_08330"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX2705752.1"
                     /translation="KKPAPDIYTWVVERLDLRPEECLAVEDSAVGLRAAVAAGLPTVV
                     TQNPYTASDDTTGALAVLADLSTVTLADLSTLHDTVHPGTQVRPGPTH"
     gene            292..1671
                     /locus_tag="VHM65_08335"
     CDS             292..1671
                     /locus_tag="VHM65_08335"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF010002.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase"
                     /protein_id="HEX2705753.1"
                     /translation="MDQSNRYADLSLTEAELIAGGKHILCAYRMKPKGGLAYLEAAAH
                     FAAESSTGTNVEVSTTDDFTKGVDALVYHVDEASEDMRIAYPLDLFDRNITDGRMMMV
                     SFLTLTIGNNQGMGDIEHAKMIDFWVPPEVIRQFDGPSMNIEDLWRILGRPVKDGGFI
                     AGTIIKPKLGLRPEPFAEAAYKFWLGGDFIKNDEPQGNQVFSPLKKTIPLVYDAMKRA
                     MDETGEAKLFSMNITADDHHEMLARADFALSTFGPDANKLAFLVDGYVGGPGMVTTAR
                     RNYPNQFLHYHRAGHGAVTSPSAKRGYTAFVLAKMARLQGASGIHVGTMGYGKMEGGT
                     DDRVIAYMIERDEAQGPVYFQRWDGIKATTPIISGGMNALRLPGFFENLGHGNLINTA
                     GGGAYGHIDGPAAGALSLRQAYEGWKAGADPVEWAATHREFARAFESFPGDADAIFPG
                     WRGRLLKTA"
BASE COUNT          312 a          659 c          599 g          259 t
ORIGIN      
        1 gcaagaagcc ggcccccgac atctacacgt gggtggtcga gcggctcgac ctgcgacccg
       61 aggagtgcct tgccgtcgag gactccgcgg tggggcttcg cgcggccgtc gcggcgggtc
      121 tgcccaccgt ggtgacccag aacccctaca ccgcgtcgga cgacaccacc ggggcgctcg
      181 ccgtcctcgc ggacctgtcg accgtgacgc tcgccgacct gtccaccctt cacgacaccg
      241 ttcaccccgg cacccaggtg cgacccgggc cgactcacta aggagcagag catggaccag
      301 tccaaccgct acgccgacct cagcctgacg gaagccgagc tgatcgccgg gggcaagcac
      361 atcctgtgcg cgtaccggat gaagccgaag gggggactcg cctacctcga ggccgccgcc
      421 cacttcgccg ccgagtcctc gaccggcacc aacgtcgagg tctcgacgac cgacgacttc
      481 accaagggcg tcgacgcgct cgtctaccac gtcgacgagg cgagcgagga catgcggatc
      541 gcctacccgc tcgacctctt cgaccgcaac atcaccgacg gccggatgat gatggtctcc
      601 ttcctcaccc tgacgatcgg caacaaccag ggcatgggcg acatcgagca cgccaagatg
      661 atcgacttct gggtcccgcc cgaggtcatc cggcagttcg acgggccgtc gatgaacatc
      721 gaggacctgt ggcgcatcct cggtcgcccg gtcaaggacg gcggcttcat cgccggcacg
      781 atcatcaagc ccaagctcgg gctgcgtccc gagccgttcg ccgaggccgc gtacaagttc
      841 tggctcggcg gggacttcat caagaacgac gagccccagg gcaaccaggt gttctcaccg
      901 ctgaagaaga ccatcccgct ggtctacgac gcgatgaagc gggcgatgga cgagaccggc
      961 gaggcgaagc tgttctcgat gaacatcacc gcggacgacc accacgagat gctcgcgcgc
     1021 gccgacttcg cgctgtcgac cttcgggccg gacgccaaca agctcgcgtt cctcgtcgac
     1081 gggtacgtcg gcgggccggg gatggtcacc actgcgcggc gcaactaccc gaaccagttc
     1141 ctgcactacc accgggcggg ccacggtgcg gtcacctccc cgagcgcaaa gcgcgggtac
     1201 accgcgttcg tgctggccaa gatggcccgt ctgcagggag cctcgggcat ccacgtcggc
     1261 acgatgggct acggcaagat ggagggcggc accgacgacc gggtgatcgc ctacatgatc
     1321 gagcgcgacg aggcgcaggg cccggtctac ttccagaggt gggacggcat caaggccacc
     1381 acgccgatca tctcgggcgg catgaacgcc ctgcgcctgc ccgggttctt cgagaacctc
     1441 ggccacggca acctcatcaa cacggcaggg ggcggcgcgt acggtcacat cgacggtccg
     1501 gcggccgggg cgctctcgct ccgccaggcc tacgagggct ggaaggccgg ggccgacccg
     1561 gtcgagtggg ccgcgacgca ccgggagttc gcccgggcgt tcgagtcctt ccccggcgac
     1621 gccgacgcga tcttccccgg ctggcgcggg cgcctgctga agaccgcgta gcgggtcggc
     1681 ggcgccacgg tgctgcgccc acggtcgagg gcctggacga cctgggccct catccgtcac
     1741 gtcgcggcgg cgtctgccgt gcttgacgac agtattggcc tcgtggaccc cctacgacac
     1801 cctgtgaccg gtttggcgca tccttcacc
//
LOCUS       DATAIX010000562         4030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_222672, whole genome shotgun sequence.
ACCESSION   DATAIX010000562 DATAIX010000000
VERSION     DATAIX010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222672"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1086
                     /locus_tag="VHM65_08340"
     CDS             118..1086
                     /locus_tag="VHM65_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX2705754.1"
                     /translation="MGRSFTAAAVQAAYVLMDRDETTEKVVQLVGEAAGMGADLIVFP
                     EVFVPGTPIWIDSVPIWDGDEAWFARLVDQSVVVPGPTTERIGAAAREAGAYVVIGVD
                     EREPHGRTIYNALLYFGPDGRLLNKHRKLMPTGSERTVWGMGDGSMLDVVPTDLGRVG
                     GLICWENYMPLARFHQYAQGVEIWVAPTLARGDGWIATMRHIAREGRVWVIGVNPCVR
                     VDQIPTDFPDRDRVWRALDGDEAEWVEPGNSVICNPNGEIVAGPLRHDEGILTAEMDL
                     DAVSAGRRLFDPVGHYHRPDVFHLSVDTRPRRAVTLITEEPTATGE"
     gene            1429..3540
                     /gene="ftsH"
                     /locus_tag="VHM65_08345"
     CDS             1429..3540
                     /gene="ftsH"
                     /locus_tag="VHM65_08345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX2705755.1"
                     /translation="MDVKRILRTPLLWIVLLVGFGLMAVSFSGGSGFTRIDTSAAEKL
                     ITEKKVDSAKLLNNEQLQLTLKDGVTYTSADGSVKEATKVFSYYVRDRGVKLVTLLET
                     NEPPKGYTDQIDEPSWIGTILVNFLPIILLLVLFWFLIGQMQGGGSRVMQFGKSKAKL
                     ATKDTPKVTFADVAGCDEAVEELYEIKEFLQEPAKFLAVGAKIPKGVLLYGQPGTGKT
                     LLARAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFEQAKTNAPAIIFVDEIDAVG
                     RHRGAGLGGGHDEREQTLNQLLVEMDGFDVKTNVILIAATNRPDILDPALLRPGRFDR
                     QIAVDAPNMIGRHRILEVHAQGKPMAADVDLLAVARRTPGFTGADLANVLNEAALFTA
                     RNNGRLIDNIALDESIDRVIAGPQKRTHIMSAKERKITAYHEGGHALVASAMNHTDPV
                     TKVTILPRGRALGYTMVMPTDDKYSTTRNEILDQLAFSLGGRVAEEIIFHDPTTGAAN
                     DIEKATSMARKMVTEFGMSERVGAIKLGQSQGEVFLGRDYGHQRDYSESVARIVDEEV
                     RRLIDNAHDEAWHVVNDNRDLLDRLVLDLLEKETLGTAELAELFQGVVKRPERPTWLS
                     SESRLLSDRPPVLTPGEAAAAHGSSNGSPPSLPPPSLPPPSLVTPTDAPAPADASMSG
                     EATAATAGIDVSDRGPADSAH"
     gene            3614..>4030
                     /gene="folE"
                     /locus_tag="VHM65_08350"
     CDS             3614..>4030
                     /gene="folE"
                     /locus_tag="VHM65_08350"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEX2705756.1"
                     /translation="MAVDHARAEAAIRELLIAVGEDPHREGLRDTPARVARAYSEIFA
                     GLDMSADEVLRTKFDIDHSELIIVRDIEVYSTCEHHLVPFHGIAHVGYIPGTDGKVTG
                     LSKIARLVDLYARRPQVQERLTSQIADALVDYLDVQG"
BASE COUNT          668 a         1421 c         1361 g          580 t
ORIGIN      
        1 ccgggttcga agggctaccg aacgtcgagg cactcttcgg ccgttgatcg gcgtcggggg
       61 tggggcgatg cggcgtcagt gacgccatac cctgagttat gaccggagga agcggcgatg
      121 ggtagatcgt tcacggccgc ggcggtgcag gcggcctacg tgttgatgga tcgggacgag
      181 accaccgaga aggtcgtgca gctcgtcggc gaggctgccg ggatgggcgc cgacctgatt
      241 gtgtttcccg aggtattcgt gccgggcaca ccgatctgga tcgattcggt cccgatctgg
      301 gacggcgacg aggcctggtt cgcccgactg gtcgatcagt ccgtggtcgt gccgggaccg
      361 acgaccgaac ggatcggcgc ggccgcccga gaggcgggtg cgtacgtcgt gatcggggtg
      421 gacgagcggg agccccacgg gaggacgatc tacaacgcgc tcctgtactt cggacccgac
      481 ggccgactgc tcaacaagca ccgcaagctg atgcccaccg gctccgagcg aactgtctgg
      541 ggcatgggcg acgggtcgat gctcgacgtc gtacccaccg acctgggaag ggtgggtggc
      601 ctcatctgct gggagaacta catgcccctg gcgcgattcc accagtacgc ccagggcgtg
      661 gagatctggg tggcccccac cctggcccgc ggcgacggct ggatcgcgac gatgcgccac
      721 atcgcccggg agggacgcgt gtgggtcatc ggggtgaacc cgtgcgtgcg agtcgaccag
      781 atccccacgg acttcccgga ccgcgaccgg gtctggcgcg ccctggacgg tgacgaggcc
      841 gagtgggtcg agccgggcaa cagcgtgatc tgcaacccta acggcgagat tgtcgcgggc
      901 cccctgcgcc acgacgaggg catcctgacc gccgagatgg acctcgacgc ggtcagcgcg
      961 gggcggcgac tcttcgaccc cgtcgggcat tatcaccgcc cagacgtgtt ccacctgtcc
     1021 gtcgacactc ggcctcggcg agcggtcacc ctcattactg aggagcccac cgccaccggc
     1081 gagtgatccg cgaatctgga gggtgagccg gtcgcgatga ggaagggccg ggcgggtcat
     1141 gtcgtcaccc acggtgacgt cgttgcggtg ggctcagggt cgggcgcgtc atgtcgtcac
     1201 cgagggtgag ctggtggcgg tgggcagacc gcgacctggt gtcggggcgg cgagggtcgc
     1261 gcccggcgag ggtcgcgccc ggccagggtc gcgcgcggcc acccgcgttg gcgagcgggg
     1321 cggcatacgg aacactgggg ttgatccggt cgttgaccgt caggtatgcc gtcgcactga
     1381 tcgcggtgca tcatcgagca ggaggaccgg ggcaggcccc gcacacacat ggacgtcaag
     1441 cgcatccttc ggaccccact gctgtggatc gtgttgctcg tcggcttcgg cctcatggcc
     1501 gtgtcgttct ccggcggctc gggcttcacc cggatcgaca cctcggcagc cgagaagctc
     1561 atcacggaga agaaggtcga ctcggccaag ctcctcaaca acgagcagct ccagctgacg
     1621 ctcaaggacg gcgtgaccta cacctccgcc gacggcagcg tcaaagaggc gacgaaggtc
     1681 ttctcctact acgtgcgcga ccgcggtgtg aagctcgtca ccctgctcga gaccaacgag
     1741 ccgcctaagg gctacaccga ccagatcgac gagccgagct ggatcgggac gatcctcgtc
     1801 aacttcctgc cgatcatctt gctgctcgtg ctcttctggt tcctcatcgg gcagatgcag
     1861 ggcggcgggt ccagggtcat gcagttcggc aagtccaagg ccaagctggc gaccaaggac
     1921 acccccaagg tgaccttcgc cgatgtcgcg ggctgtgacg aggcggtcga ggagctgtac
     1981 gagatcaagg agttcctcca ggagccggcc aagttcctcg cggtgggcgc caagatcccc
     2041 aagggcgtgc tcctctacgg gcagccgggc accggcaaga ccctgctcgc ccgggccgtg
     2101 gccggggagg cgggcgtccc gttctactcg atctccgggt cggacttcgt cgagatgttc
     2161 gtcggcgtcg gcgcctcgcg ggtccgtgac ctgttcgagc aggccaagac caacgccccg
     2221 gccatcatct tcgtcgacga gatcgacgcc gtcgggcgcc accgtggcgc cggtctcggc
     2281 ggcggccacg acgagcgcga gcagaccctc aaccagctgc tcgtcgagat ggacggcttc
     2341 gacgtcaaga ccaacgtcat cctcatcgcg gcgacgaacc gccccgacat cctcgacccg
     2401 gcgctgctgc gaccggggcg cttcgaccgg cagatcgccg tcgacgcccc caacatgatc
     2461 gggcggcacc ggatcctcga ggtccacgcc cagggcaagc cgatggccgc cgacgtcgac
     2521 ctgctcgccg tcgcccggcg cacgcccggc ttcaccggcg cggacctggc caacgtcctc
     2581 aacgaggcgg cgctgttcac cgcacgcaac aacggccggc tcatcgacaa catcgcgctc
     2641 gacgagtcga tcgaccgggt catcgccggg ccacagaaac gcacccacat catgagcgcc
     2701 aaggagcgca agatcaccgc ctaccacgag ggtgggcacg ccctggtcgc ctcggcgatg
     2761 aaccacaccg acccggtgac caaggtgacg atcctgcccc gcggccgggc cctcggctac
     2821 acgatggtca tgccgaccga cgacaagtac tcgacgaccc gcaacgagat cctcgaccag
     2881 ctcgcgttct ccctgggagg gcgcgtcgcc gaggagatca tcttccacga cccgaccacg
     2941 ggtgcggcca acgacatcga gaaggccacg tcgatggcgc gcaagatggt caccgagttc
     3001 ggcatgtccg agcgggtcgg ggcgatcaag ctcggccaga gccagggcga ggtcttcctc
     3061 ggccgtgact acggccacca gcgcgactac tccgagtcgg tcgcccgcat cgtcgacgag
     3121 gaggtccgcc ggctcatcga caacgcccac gacgaggcct ggcatgtcgt caacgacaac
     3181 cgcgacctgc tcgaccggct cgtcctcgac ctgctcgaga aggaaaccct cgggacggcc
     3241 gagctcgccg agctcttcca gggcgtcgtc aagcggccgg agcgcccgac ctggctctcc
     3301 agcgagagcc ggctcctctc ggaccgccca ccggtgctca ccccgggcga agcggccgcg
     3361 gcgcacggca gctcgaacgg ctcaccgccc tcactccctc cgccgtcgct ccctccgccg
     3421 agcctggtga cgccgaccga cgcgcccgct ccggccgacg cgagcatgag cggcgaggcg
     3481 accgcggcga cggccggtat cgacgtctcc gaccgcggac cggccgactc cgcgcactga
     3541 cgcggcgccg agccagcccg gcgccgcgtc atacctgctc gtccgcacct cgccacctgg
     3601 aagggaccac cccatggcgg tagaccacgc ccgcgccgag gcggccatcc gcgagctgct
     3661 catcgccgtc ggcgaggacc cgcaccgcga ggggctgcgg gacaccccgg cgcgggtcgc
     3721 gcgcgcctac tccgagatct tcgccgggct cgacatgtcg gccgacgagg tgctgcggac
     3781 gaagttcgac atcgaccaca gcgagctcat catcgtgcgc gacatcgagg tctactcgac
     3841 ctgcgagcac cacctcgtgc cgttccacgg gatcgcgcac gtcggctaca tccccgggac
     3901 cgacggcaag gtcaccgggc tgtccaagat cgcccggctc gtcgacctct atgcccgccg
     3961 cccgcaggtt caggagcggc tcacctcgca gatcgccgac gcgctcgtcg actatctcga
     4021 cgtccagggc
//
LOCUS       DATAIX010000563         1695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_193107, whole genome shotgun sequence.
ACCESSION   DATAIX010000563 DATAIX010000000
VERSION     DATAIX010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193107"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..496
                     /locus_tag="VHM65_08355"
     CDS             65..496
                     /locus_tag="VHM65_08355"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEX2705757.1"
                     /translation="MRRITLALVSTLSALILLFSYHTSTAGPARTVQAAGADPGTSDS
                     SGSAGSAGATTYTGDAVNTRWGIVQVEITVADAKITRAEAIQYPQENPRDQEINSYAI
                     PLLNEEAVQAQSAQLDSIGGATVTVQGYIASLQSAIDQAHL"
     gene            556..1236
                     /locus_tag="VHM65_08360"
     CDS             556..1236
                     /locus_tag="VHM65_08360"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX2705758.1"
                     /translation="MPISITLRGGGPDSAGAVAAVEESFAILREVDTVFSTHRPESEI
                     SRLRRGELDLAGACPQVREVLELCALATELTDGWFSALLPAPDGERVLDPTGLVKGWA
                     MERACRPLARLDGLDYCLNAGGDIAVGGTVGGIWRLGIEDPRDRTRVGHVVVLARGGM
                     ATSGTAARGAHLVDPATGERVTRAGSVTVVGPSPMWADVWATALFVAPADLAHRLPAG
                     DGYSVIRL"
     gene            complement(1226..>1695)
                     /locus_tag="VHM65_08365"
     CDS             complement(1226..>1695)
                     /locus_tag="VHM65_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705759.1"
                     /translation="AEPAGASALAGALLTYSALTGSTRHRDAAEAALQTAAEIAARDP
                     RFAGWWLAVAEAVVAGPLQVAVVRPVQPEAGAEPTPRTPPRQGLWRIAVGSTSPGLVA
                     VQGRPDQPGIPLLASRPVRRGRETAYICRGFVCDAPLVEPAEVAAALARRPRA"
BASE COUNT          235 a          634 c          616 g          210 t
ORIGIN      
        1 tcgagcgctt cagctactga cgacgccggc ccgaccaccc cgatcgcgaa cctaggagcc
       61 cacgatgcgc cgcatcaccc tcgccctggt gagcaccctg tcggcgctca tcctgctgtt
      121 cagctaccac acgagcaccg ccgggccggc caggaccgtc caggcggccg gggctgaccc
      181 gggaacgagc gactcgtcgg ggtccgccgg ctccgccggt gcgacgacct acaccggcga
      241 cgcggtcaac acccggtggg gcatcgtcca ggtcgagatc accgtcgccg acgccaagat
      301 caccagggcc gaggcgatcc agtaccctca ggagaacccc agggaccagg agatcaactc
      361 ctatgccatc cccctcctca acgaggaagc cgtccaggct cagagtgccc agctcgacag
      421 catcggcggc gccacggtga ccgtccaggg ctacatcgcc tcgctgcagt cggccatcga
      481 ccaggcccac ctgtgaccgc tcgcatcccg accgacactg cacgcaagcg ggcgcgggtc
      541 gagttcagca tggggatgcc gatcagcatc accctgcggg gaggcggccc cgactccgcc
      601 ggggcggtgg ctgccgtcga ggagtccttc gcgatcctgc gcgaggtcga caccgtcttc
      661 tcgacgcacc ggccggagag cgagatcagc cgcctccgac gcggggagct cgacctggcc
      721 ggcgcgtgtc cccaggtccg ggaggtgctc gagctgtgcg ccctggccac cgagctcacc
      781 gacgggtggt tctcggcgct gctgccggca ccggacgggg agcgggtgct cgacccgacc
      841 ggcctggtca agggctgggc gatggagcgt gcctgccggc cgctggcccg ccttgacggg
      901 ctggactact gcctcaacgc cggcggggac atcgcggtcg gcggcacggt cggggggatc
      961 tggcggctcg gaatcgagga cccgcgcgac cggacccgcg tcggccatgt cgtcgtcctc
     1021 gcccgcgggg gtatggcgac ctcgggcacg gctgcccgtg gcgcccatct cgtcgacccg
     1081 gccaccgggg agcgggtcac ccgggccggg tcggtcaccg tggtcggccc gtcaccgatg
     1141 tgggccgacg tctgggccac ggccctcttc gtcgccccgg cggacctggc gcaccgcctc
     1201 cccgccggcg acggctacag cgtcatcagg ctctaggacg acgggccagg gcggcagcca
     1261 cctcggccgg ctcgacgagg ggagcgtcgc agacgaaacc ccggcagatg tatgccgtct
     1321 cccgtccgcg ccggacggga cggctggcca gcagcggtat gcccggctgg tccggccggc
     1381 cctggacagc cacgagaccg ggggacgtcg acccgacggc gatgcgccag agcccctgcc
     1441 gcggcggggt cctcggtgtg ggctcggcgc cagcctcggg ctggaccggg cggacgacgg
     1501 cgacctggag cgggcccgcg acgacggcct cggccaccgc gagccaccag ccggcgaacc
     1561 gggggtcacg ggcggcgatc tcggcggcgg tctgcagggc cgcctcggcc gcgtcgcggt
     1621 ggcgggtcga tcccgtcagt gccgagtagg tgagcagcgc ccctgccagg gcgctcgctc
     1681 cagcgggttc ggcgt
//
LOCUS       DATAIX010000564         1543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_132957, whole genome shotgun sequence.
ACCESSION   DATAIX010000564 DATAIX010000000
VERSION     DATAIX010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132957"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1543
                     /gene="pknB"
                     /locus_tag="VHM65_08370"
     CDS             <1..>1543
                     /gene="pknB"
                     /locus_tag="VHM65_08370"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEX2705760.1"
                     /translation="FRREAHSAASLNHPNIVAIYDSGEDSVVENGGTEAKVPYIVMEY
                     VAGQTLREKLDAAHVLAPDEAARITQRILDALAYSHRRGIVHRDIKPANVMISITGEV
                     KVMDFGIARAIADTAATMTQTHSVIGTAQYLSPEQGLGSAVDSRSDLYSTGCLLYELL
                     TGRPPFRGDSPVAMVFQHVNEAPQPPSTFAPRVSPGYDAIVLHALEKDRDSRYRSAEE
                     FRADLANARSGMPVSAAALGSANEDPASATMVLPSRASRHDPVPAPMTFPAQESAEQP
                     PGRGRGVFVLLLLAALLAVAAGGLGLKYLLGGSTDTTQVKVPYVIGMSQADAERLLTS
                     RGLVPKVVAVSGTTFAPGLVADQRPKKDESVASGGRVEIDVSSGPESVAVPDVEGLTE
                     AEARTLLETNGLRVTQIVLVNDNAQKKGRVIEADPAIGQIVKTGDAITLRIASGKVPV
                     PDLTRKDVTVVLQLLAAKDLELATPEYVITSETADGLVLEQTPAPDALVDVGSTVKVK
                     VAKAPP"
BASE COUNT          243 a          573 c          517 g          210 t
ORIGIN      
        1 ggttccggcg cgaggcgcac tcggccgcga gcctgaacca cccgaacatc gtcgcgatct
       61 acgactccgg cgaggactcg gtcgtcgaga acggcggcac cgaggccaag gtcccctaca
      121 tcgtcatgga gtatgtcgcg ggccagaccc tgcgcgagaa gctcgacgcc gcccacgtcc
      181 tcgcccccga cgaggccgct cggatcaccc agcggatcct cgacgcgctg gcctacagcc
      241 accgtcgagg gatcgtccac cgcgacatca agccggccaa cgtcatgatc tcgatcaccg
      301 gtgaggtcaa ggtcatggac ttcgggatcg cccgggcgat cgccgacacc gccgccacga
      361 tgacccagac ccactcggtc atcgggacgg cgcagtacct ctcgcccgag cagggtctgg
      421 gctcggccgt cgactcgcgc tcggacctct actcgacggg gtgcctgctc tacgagctgc
      481 tcaccggccg gccgccgttc cgcggtgaca gccccgtggc gatggtcttc cagcacgtca
      541 acgaggcgcc ccagcctccg tcgaccttcg cgccgcgggt ctcgcccggg tatgacgcca
      601 tcgtcctgca cgccctcgag aaggaccggg actcccgcta ccgcagtgcc gaagagttcc
      661 gggccgacct ggccaacgcc cgcagcggta tgccggtcag cgcggcggcc ctggggtcgg
      721 cgaacgagga cccggcctct gcgacgatgg tgctgccctc ccgcgccagc cggcatgacc
      781 ccgtgcccgc gcccatgacg ttccccgcgc aggagtcggc cgagcagccc ccgggccggg
      841 gcaggggcgt cttcgtcctg ttgctcctgg cggcgctgct ggctgtggcc gccggcggac
      901 tcggcctgaa gtacctcctc ggcggttcga ccgacacgac ccaggtcaag gtcccctacg
      961 tcatcgggat gagccaggcc gacgccgagc ggctgctgac ctcacggggg ctggttccca
     1021 aggtcgtcgc cgtgtcaggc acgaccttcg ccccgggcct ggtcgccgac cagcggccca
     1081 agaaggacga gtcggtggcc tccgggggcc gggtcgagat cgatgtgtcc agcgggccgg
     1141 agagcgtggc agtgcccgac gtcgaggggc tgaccgaggc cgaggcccgc accctgctgg
     1201 agaccaacgg gcttcgggtc actcagatcg tcctggtcaa cgacaacgcc cagaagaagg
     1261 gccgggtcat cgaagcggac ccggcgatcg ggcagatcgt caagaccggc gacgcgatca
     1321 cgctgcggat cgcgtccggc aaggtccccg tgcctgacct gacccgcaag gacgtcacgg
     1381 tggtgctgca gctcctggcc gccaaggacc tcgagctggc gacacccgag tacgtcatca
     1441 cgagcgagac ggccgacggc ctggtcctcg agcagactcc agcccccgac gcgctcgtcg
     1501 acgtcggctc cacggtcaag gtcaaggtcg ccaaggcccc gcc
//
LOCUS       DATAIX010000565         1957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_223009, whole genome shotgun sequence.
ACCESSION   DATAIX010000565 DATAIX010000000
VERSION     DATAIX010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223009"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VHM65_08375"
     CDS             <1..315
                     /locus_tag="VHM65_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705761.1"
                     /translation="LLSPALRFSTSADLARWAESGKPVVCLVPELDDYLRPAEARERF
                     TAIPQAEVIGVAGAKHLWVGEPNVRRVLDEITARVNPGATPLPHTWPAGEQTHERSLL
                     RH"
     gene            659..>1957
                     /locus_tag="VHM65_08380"
     CDS             659..>1957
                     /locus_tag="VHM65_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705762.1"
                     /translation="MALLDDAFSVPPSSALARPRLLTLLSSVERTGLALVVAPVGYGK
                     TTLLGQWSAARGGVVRWYEPNRLPSRDAVRRDLDSTPMLRGIVVDDVHRIGGVAAAAR
                     LERLIEDVAPEVPVVLGARRLPAFNLARHEFPRAIVITTRDLSFRPWEVAELFADVYA
                     DPLQNEEPLAVADRTEGWAAALRLWHLARRMDREDVPGEQPPDCVRAYLARQVLNGLD
                     PELLRLMRTSSVFDVVTADRVDRLMGIGNGHRLLIDLARRVDLVTVRPDGRSYRYHRT
                     LRDHLRAELAEELGPSATRALFDDAAVVLEELGDLPEALASRCRAQNSRDAARLLVFH
                     GRGLTDAPGDWLDMLSASMAGAEPQVRLARARLLLADGMTREAETEATTAACHLTDLE
                     DRAYCELVASQARLRRAGFATKEPAGPPSPAVEAVRLLVQG"
BASE COUNT          285 a          713 c          640 g          319 t
ORIGIN      
        1 ctgctcagcc cggccctgcg gttcagcacc tccgcggacc tcgctcggtg ggccgagtcc
       61 ggcaagccgg tcgtctgcct cgtgcccgag ctcgatgact acctgcgccc tgccgaggcc
      121 cgggagcgct tcacggcgat cccgcaggcc gaggtcatcg gggttgccgg ggccaagcac
      181 ctctgggtcg gtgagcccaa cgtgcgcagg gtcctcgacg agatcaccgc ccgggtcaac
      241 ccgggcgcca ccccgctgcc ccacacctgg ccagcggggg agcagaccca cgagcgctca
      301 ctactgcgcc actgacgtca cgcggggtgg ccctcgagcc agtccggtgg cgctcggtcc
      361 aaatcatgcc accaggctcc gccctgatgg acaggtggtc ccgagacgca cctccctggc
      421 agcctcacgc gacccacaac aagcagcctt cagggggcaa gtcttgcacc gaccatgaga
      481 gctgccgccg aatgaccggt agaacgccgt tgcagcaaac agcccggtct tgtttaatca
      541 acaggacata tgagtggtca atcagtacca tccagatcat cgcgtggttg ggggtatttc
      601 taaggcagca actgccctct tcaatacgag acccaatctc cacgtctgga ggcccaccgt
      661 ggctttgctt gacgatgcct tctcggtgcc tccctcgtcg gccttggcac gcccgaggct
      721 gctcacgctg ctcagcagcg tcgaacgcac cgggctcgcc ctggtcgtag cgcccgtcgg
      781 ctacgggaag accaccttgc tgggtcagtg gagcgcggcc cggggtggcg tcgtgcgctg
      841 gtacgagccc aaccggctgc cgtcccggga cgcagtgcgc cgcgacctcg actcgacccc
      901 gatgctgcgc ggcatcgtcg tcgatgatgt ccaccggatc gggggggtcg cggccgcggc
      961 gcggctagag cggctgatcg aggacgtcgc accggaggtc cctgtcgtcc ttggcgcgcg
     1021 acgcctgccc gcgttcaacc tcgcccggca cgagttcccc cgagcgatcg ttatcaccac
     1081 tcgggacttg tcgttccgac cgtgggaggt cgctgagctc tttgccgacg tgtatgccga
     1141 ccctctccag aatgaggagc cgcttgccgt ggcggaccgc accgaaggct gggccgctgc
     1201 tcttcgcctg tggcacctcg cccgccggat ggaccgcgag gatgttcccg gcgagcagcc
     1261 gccggactgc gtccgcgcct acctggcccg ccaggtcctg aacggcctcg atcctgagct
     1321 cttgcgcctg atgcgcacct cgagcgtgtt cgatgtcgtc accgccgacc gggtagaccg
     1381 gcttatgggg atcggcaacg gtcaccggct gctcatcgac ctggccaggc gggtcgacct
     1441 cgtcaccgtc cgaccggacg gccggagcta ccgctaccat cgcaccttgc gcgaccatct
     1501 ccgtgccgag ttggctgaag agctggggcc gtctgcgacg cgtgccctct tcgacgacgc
     1561 agcggtcgtt ctcgaggagc tcggcgatct gcccgaggct cttgcctccc gatgccgcgc
     1621 acagaactcc cgtgacgcgg cccggctgct ggtcttccac ggtcgggggc tcacggatgc
     1681 gccgggcgac tggctcgaca tgctttccgc ctcgatggcc ggggccgaac cgcaggtacg
     1741 tctcgcgcgc gcccggctgc tgctggcgga cggcatgacc cgcgaggccg agaccgaggc
     1801 cacaacagcg gcctgccacc tcaccgacct ggaggaccgg gcctactgtg agctggtggc
     1861 cagccaggct cgcctgcggc gggccgggtt cgcgaccaag gagcccgcag gacccccttc
     1921 gccagccgtc gaggcggtcc gcctccttgt ccagggc
//
LOCUS       DATAIX010000566         2234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_281962, whole genome shotgun sequence.
ACCESSION   DATAIX010000566 DATAIX010000000
VERSION     DATAIX010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281962"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VHM65_08385"
     CDS             <1..455
                     /locus_tag="VHM65_08385"
                     /inference="COORDINATES: protein motif:HMM:NF020752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HEX2705763.1"
                     /translation="SGPAPVAGAPATIGEAPVVGFSADFRAALDDDLNVSGALAAVHE
                     RVREGNTALDEGEDDEAVRAARDVIAMTEVLGVNPVDPRWSGGGSAHSPARLALAALV
                     RAQLGTRQAARTARDYASADAVRDQLAAAGIAIEDTPTGARWSLVREL"
     gene            468..1466
                     /gene="rlmB"
                     /locus_tag="VHM65_08390"
     CDS             468..1466
                     /gene="rlmB"
                     /locus_tag="VHM65_08390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX2705764.1"
                     /translation="MPGNSQRRGAVRKSAKGASAGSGGLRRKGLEGRGPTPKATEREY
                     HPAAKRAARGGRVTNPAGRSTGRDGGGPSRRPGGGGARRAKGSSEIVAGRNSVVEALR
                     ADIPATTMYVAGRIDADDRVREAIKTATTRGIPILETPRGELDRLTDGAVHQGLALQV
                     PPYDYAEPADLVDPPMPGIPLVVALDGITDPRNLGAIIRSVAAFGGHGVVVPERRSVG
                     MTASAWKTSAGAAARVPVAKAANLTRALEDFKRAGFFVLGLDMEGDVDLPAITLADEP
                     LVIVVGSEGKGLSRLVRQTCDQIVSIPMSSAVESLNAGIATGVTLYEVARRRAATG"
     gene            1557..>2234
                     /locus_tag="VHM65_08395"
     CDS             1557..>2234
                     /locus_tag="VHM65_08395"
                     /inference="COORDINATES: protein motif:HMM:NF017831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF929 family protein"
                     /protein_id="HEX2705765.1"
                     /translation="MSPPPAGQVTRPAVLDEVWEDEVVGKSARTRTASNAKLAAIKAE
                     QARKDRRRKVLLASGVIVVVLAVAAAIVVASRNSPAKAPTTASALDPTAFKNLTSVPG
                     AAFNTVGVGSAAAAAAPAKVDAPALTKDGKPRVVYVGAEYCPFCASERWALTVALSRF
                     GTWSTLAAASSSPEDTYPSTPTVSFHGATYTSDYLSFTGYETSTNKIVNGVREKLDTL
                     SPEDETMF"
BASE COUNT          324 a          839 c          794 g          277 t
ORIGIN      
        1 cctccggccc ggcgcccgtg gccggggccc ccgccacgat cggcgaggca cccgtcgtgg
       61 gcttcagcgc ggacttccgt gccgctctcg atgacgacct caacgtctcc ggtgcgctgg
      121 ccgcggtcca cgagcgggtc cgcgagggca acaccgcact cgacgagggc gaggacgacg
      181 aggcggtccg cgccgcccgg gacgtcatcg ccatgaccga ggtgctcggg gtcaacccgg
      241 tcgacccgcg ttggtccggc ggtggctcgg cccactcgcc ggcccggctc gccctggccg
      301 ccctcgtccg ggcccagctc ggcacccgcc aggcggcccg gacggcccgc gactacgcga
      361 gcgccgacgc ggtccgcgac cagctggcgg cggccgggat cgccatcgag gacaccccca
      421 ccggcgcccg ctggtcgctg gtccgtgagc tctgatcgga gccgtcaatg ccaggcaact
      481 cccagcggcg aggagccgtc cgcaagtccg ccaagggcgc gtcggccggc tccggtggcc
      541 tgcgccgcaa gggcctggag ggccgcgggc ccacccccaa ggcgaccgaa cgcgagtacc
      601 atccggcggc caagcgggcc gcgcgcggcg gtcgcgtcac caaccccgcc gggcggtcca
      661 cgggtcggga cggcggcggc ccgagccggc ggcccggcgg aggcggcgct cgccgggcca
      721 aggggtccag cgagatcgtc gccggtcgca actccgtcgt cgaggcgctg cgagccgaca
      781 tccccgccac gacgatgtat gtcgcgggcc ggatcgacgc cgacgaccgg gtccgcgagg
      841 cgatcaagac cgcgacgacc cgcggtatcc cgatcctgga gaccccccgc ggcgagctcg
      901 accggctcac cgacggcgcc gtgcatcagg gactggcact gcaggtgccg ccctacgact
      961 acgccgagcc ggccgacctc gtcgacccgc cgatgcccgg catacctctt gtcgtcgccc
     1021 tggacgggat caccgaccca cgcaacctcg gcgcgatcat ccgctcggta gccgcgttcg
     1081 gcgggcacgg cgtcgtcgtg ccggagcgcc ggtcggtcgg catgaccgcg tccgcgtgga
     1141 agacctcggc cggtgctgcc gcccgggtgc ccgtcgccaa ggccgcgaac ctgacccggg
     1201 ccctcgagga cttcaagcgg gcggggttct tcgtcctcgg cctcgacatg gagggagatg
     1261 tcgacctccc agcgatcacc ctggccgacg agccgctcgt catcgtcgtc gggtcggagg
     1321 gcaagggcct gtcgcggctc gtgcgccaga cctgcgacca gatcgtctcg atcccgatgt
     1381 cctcggcggt cgagtcgctc aacgccggga tcgccaccgg ggtgacgctc tacgaggtgg
     1441 cccggcggcg cgccgcgacc gggtgatgcc gaccgggatg ccgcaccggt gacggaggga
     1501 acttcgccgg tatgccgatg cgttggtctg cgtggggacc gcccggttag gctgcggtga
     1561 gtccgccacc ggccggacag gtaacaagac cagcggtgct ggacgaggtg tgggaggacg
     1621 aggtcgtggg caagagcgca cgcacacgca cagcgagcaa tgccaagctc gcggcgatca
     1681 aggccgagca ggcacgcaag gaccgccggc gcaaggtgct gctggccagc ggcgtcatcg
     1741 tcgtcgtcct cgccgtcgcg gcggccatcg tggtcgcgag caggaacagt ccggccaagg
     1801 cacccaccac ggcgagcgcc ctcgatccca cggccttcaa gaacctcacc tccgtccctg
     1861 gtgcggcgtt caacacggtc ggcgtgggct cggcggcagc cgcggcggcc cccgcaaagg
     1921 tggacgcccc ggcactgacc aaggacggca agccgcgcgt cgtctacgtc ggtgccgagt
     1981 actgcccctt ctgcgcgtcc gagcgctggg cgctgacggt ggctctgtcg cggttcggca
     2041 cgtggtccac gctcgccgcg gcgagctcgt cgcctgagga cacctacccc agcaccccga
     2101 cggtgtcctt ccacggcgcg acctacacca gcgactacct cagcttcacg gggtacgaga
     2161 ccagcaccaa caagatcgtc aacggcgttc gcgagaagct cgacaccctg agccccgagg
     2221 acgagacgat gttc
//
LOCUS       DATAIX010000567         2854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_223234, whole genome shotgun sequence.
ACCESSION   DATAIX010000567 DATAIX010000000
VERSION     DATAIX010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223234"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VHM65_08400"
     CDS             <1..545
                     /locus_tag="VHM65_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705766.1"
                     /translation="PAVGTPSPPPPEVAPVNVTALIGEAMNKSGLLWIDVPGDRAWPA
                     WHAWASDTAYVINGPGEQTLPWLPPEVTLLLRSKDTGGRLLRVAARAEVLPADSAAYA
                     PAVEALRASRLNAPADVEDRWRAECTVTALRPYGPLVEGPGNMPTGNHIAKPPPTPAT
                     TATWLPKHLRGRPQRRRGTH"
     gene            598..1878
                     /locus_tag="VHM65_08405"
     CDS             598..1878
                     /locus_tag="VHM65_08405"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX2705767.1"
                     /translation="MTPATRVFTARLVGLSVFDPLGDQVGRVKDVIVSFGAGTTRPRV
                     IGLVVEVAGRRRVFVPMTRVTSLDGGQVITTGLVNMRRFEARPTETLVFAELLDRRIT
                     VTGPEGEFTGVVEDLAMVETRRREWALSKVFCRKDLPGRSGAISRFTRRRGPTVLVDL
                     EQVSGLHEQVDAQSTERLLLSLDGLRVADMAEAIRELTPKRRAEVAAALDDEKLADVL
                     EELPEDDQVEIIAALALERAADVLEEMQPDDAADLLSELPKDQAERLLALMEPDEAAP
                     LRRLLSYDDNTAGGMMTSEPVILGPQATIAEALAVVRQEELPPALASTVFVCRPPLET
                     PTGTFLGMAHIQRLLREPPHGAIGSVLDRAVEPLRPDTPLGVVTRELATYNLMSLPVV
                     DEAGRLLGAVTVDDVLDHILPQDWREERHEVTHG"
     gene            1871..2407
                     /locus_tag="VHM65_08410"
     CDS             1871..2407
                     /locus_tag="VHM65_08410"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX2705768.1"
                     /translation="MAERRPDSGRLDQPREARQNFLPRGSITQETFGVLSERFARFMG
                     TPYFLIYMTGFVLCWVAWNILAPSDVRYDPYPFIFLTLMLSLQASYAAPLILLAQNRQ
                     ADRDRVTLEQDRDGDERNLADTEFLTREVAALRIALRDTATRDFVRSELRDLITELEE
                     RGLAGPAVSAQRRREAPP"
BASE COUNT          408 a         1081 c          958 g          407 t
ORIGIN      
        1 gccctgctgt cgggacaccg tcgcctcccc cgcccgaggt ggccccggtc aacgtcacgg
       61 cactcatcgg tgaggcgatg aacaagtccg ggctgctctg gatcgacgtc cccggggacc
      121 gtgcctggcc ggcctggcac gcctgggcga gcgacacggc atacgtcatc aacggccctg
      181 gcgagcagac cctgccgtgg ctcccgccgg aggtgacgct gcttcttcgc agcaaggaca
      241 ccggcggccg gctgctgcgt gtcgcggccc gcgccgaggt gctccccgcc gacagcgccg
      301 cgtatgctcc cgcggtcgag gcgctacgcg cctcgcggct caacgcgccg gctgacgtcg
      361 aggaccggtg gagggccgag tgcaccgtga cggcgcttcg cccctacggc ccgctcgtcg
      421 aaggcccggg caacatgccc accggcaacc acatcgccaa gcccccgccg accccggcga
      481 cgacggccac gtggcttccc aagcatctgc gcggccgacc gcagaggcgg cgcggcacgc
      541 actgacgccg gctcgcgcgc gagccctatc ggcgcgaccg gcggctagcc ttgggccatg
      601 acccctgcga cgcgcgtctt caccgcgcgg ctcgtcgggc tcagtgtctt cgacccgctc
      661 ggtgaccagg tcggccgggt caaggacgtc atcgtcagct tcggcgccgg gacgacgcgc
      721 ccgcgcgtca tcgggctcgt cgtcgaggtc gccgggcgcc gccgggtgtt cgtcccgatg
      781 acccgggtca cctcactcga cggggggcag gtcatcacga ccggtctggt caacatgcgc
      841 cggttcgagg cccgcccgac cgagactctc gtcttcgccg agctgctcga ccggcgcatc
      901 accgtgaccg gtcccgaggg ggagttcacg ggagtggtcg aggacctcgc catggtcgag
      961 acccgccggc gcgagtgggc cctgtccaag gtgttctgcc gcaaggacct gccgggccgg
     1021 tccggcgcca tctcgcgctt cacccggcgc cgggggccga ccgtcctcgt cgacctcgag
     1081 caggtatcgg ggctgcacga gcaggtcgac gcccagtcga ccgagcggct gctcctgtcc
     1141 ctcgacggcc tgcgggtcgc cgacatggcc gaggcgatcc gcgaactcac cccgaagcgg
     1201 cgcgccgagg tcgccgcggc cctcgacgac gagaagctcg ccgacgtcct cgaagagctc
     1261 cccgaggacg accaggtcga gatcatcgcg gccctggccc tcgagcgcgc cgcggacgtc
     1321 ctcgaggaga tgcagcccga cgacgcggcc gacctgttgt ccgagctgcc caaggaccag
     1381 gccgagcggc tgctcgccct catggagccg gacgaagccg ctccgctgcg gcggctgctc
     1441 agctacgacg acaacaccgc cgggggcatg atgacctccg agccggtgat cctcggaccg
     1501 caggccacga ttgccgaagc cctcgccgtt gtccgccagg aggagctgcc accagccctc
     1561 gcgtcgaccg tcttcgtctg ccgtcccccg ctggagaccc cgaccggcac cttcctcggg
     1621 atggcccaca tccagcgcct gctgcgcgag ccaccccacg gggccatcgg atctgtcctc
     1681 gaccgtgccg tcgagccctt gcgtcccgac accccgctcg gtgtggtgac ccgcgagctc
     1741 gcgacataca acctcatgtc gttgcctgtc gtcgacgagg cgggacggct cctcggcgcg
     1801 gtgaccgttg acgacgtgct cgaccacatc ctcccgcagg actggcgcga ggaacggcac
     1861 gaggtgaccc atggctgagc ggcgtcccga ctcgggccgc ctcgaccagc cccgcgaggc
     1921 acgccagaac ttcctccccc gcggctcgat cacccaggag acgttcgggg tcctgagcga
     1981 acgcttcgcc cgcttcatgg ggacgccgta cttcctcatc tatatgaccg ggttcgtcct
     2041 gtgctgggtg gcgtggaaca tcctcgcgcc gagcgacgtc cgctacgacc cctacccgtt
     2101 catcttcctc accctcatgc tgagcctgca ggcgtcgtat gccgcgccgc tgattctgct
     2161 cgcgcagaac cggcaagccg accgtgaccg agtgacgctc gagcaggacc gagacggcga
     2221 cgagcgcaac ctcgcggaca ccgagttcct cacccgggag gtcgcggccc tgcgaatcgc
     2281 cctgcgtgac acggcgactc gtgacttcgt gcgctccgag ctgagggacc tgatcaccga
     2341 gctggaggag cgcggcctcg ccgggccagc agtctcggca cagcggcgaa gggaggctcc
     2401 cccgtagaag ggcgcacctg cgacgtccca gtcgagcccc tgtgcccggt gcgtcccgtg
     2461 ccgccgagct gcccgtggcg atggccgcct cggccgccag ccggcccccc agggagtgcc
     2521 cggcatactc cagcttgtcg ccctctgggc cgcgcgcagc cttgacaagc tgggccagcg
     2581 tgatcgcttc tcggtcctgg cccgttacgc cgacgacacc gacgaggtcg gtgccaatat
     2641 cactgggggt ggggatgtcg gtgcccgcaa aggcgttggt gcctcgaaaa gctgaggacg
     2701 cacctccccg tggagtcgac gaagagcgtg gccgagaacc cggtggcggg atcgccgagg
     2761 tccctcacct tggaccaccc cgggggtggc tcccgatcgc cgtacgccgc agtggagagc
     2821 cccagcaagt cgtgggcgtg ccccgtccgt cccg
//
LOCUS       DATAIX010000568         1928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_252056, whole genome shotgun sequence.
ACCESSION   DATAIX010000568 DATAIX010000000
VERSION     DATAIX010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252056"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="VHM65_08415"
     CDS             complement(<1..1339)
                     /locus_tag="VHM65_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705769.1"
                     /translation="MSARIEQPYHYQQRDLVEPDWARLPGYRGVTATEWADVQWQRSH
                     CVKNVRQLRELMGDLLTDELYADLERDQAERATMSMLVTPQMMNTMVPSTDEPMPTAG
                     TDFTAAFYADPIRRYMLPVFSDRRSDWPSHPHSTRDSLHEHDMWVAEGLTHRYPTKVL
                     AEMLPTCPQYCGHCTRMDLVGNSTPVVAKLKFDGKPMDRYAAMLAYLQATPTVRDVVV
                     SGGDVANMPWRNLEGFLDKLIEIDSIRDIRLATKALMGLPQHWVAPDVVEGIGRIATK
                     ARRRGVHVAIHTHINNVASVTPAVAGAAHAMLDAGLFNIRNQGVLMRGVNDTPEQLLD
                     LCFALQDRAAITPYYFYMCDMIPFAEHWRLSLAEAQDLQHSIMGYLPGFATPRIVCDV
                     PFVGKRWVHQQDSYDSEHGISYWRKNYRTSIEGADTAALSREFVYYDPTRARCR"
     gene            1426..>1928
                     /locus_tag="VHM65_08420"
     CDS             1426..>1928
                     /locus_tag="VHM65_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705770.1"
                     /translation="MSPYDETAPRAGASPIGLHRVLEPTGDAVTLPQMARRIDPDPRI
                     WPDEVRVDVDTLNLDAASYRQLATKHTRPDGTVDGDAVRAEVLDIVATRGKMQNPVTG
                     SGGMLIGRVAEVGPDSTLGLAPGDRIATLVSLSLTPLQITDRLARWDGLAEQVPTAGT
                     AILFGRS"
BASE COUNT          297 a          627 c          687 g          317 t
ORIGIN      
        1 atctacacct tgcccgagtc gggtcgtagt agacgaactc gcggctcagt gccgcagtgt
       61 ccgccccctc gatcgaggtg cggtagttct tgcgccagta ggagatcccg tgctcgctgt
      121 cgtaggagtc ctgctggtgc acccagcgct tgccgacgaa gggcacgtcg cagacaatgc
      181 gcggcgtcgc gaagccgggc aggtagccca tgatcgagtg ctgcaggtcc tgggcctcgg
      241 cgagggagag ccgccagtgc tccgcgaacg ggatcatgtc gcacatgtag aagtagtagg
      301 gcgtgatcgc ggcccggtcc tgcagggcga agcagaggtc gagcagctgc tcgggcgtgt
      361 cgttgacccc ccgcatgagc acgccctggt tgcggatgtt gaacagcccg gcatcgagca
      421 tcgcgtgtgc ggcgccggcc acggccggtg tcaccgaggc gacgttgttg atgtgcgtgt
      481 ggatcgcgac gtggaccccg cgccgccggg ccttggtcgc gatgcgcccg atgccctcga
      541 cgacgtccgg tgccacccag tgctgcggca ggcccatgag cgccttggtc gcgaggcgga
      601 tgtcacggat gctgtcgatc tcgatcagct tgtcgaggaa gccctcgagg ttgcgccacg
      661 gcatgttggc gacgtcaccg ccggagacga cgacgtcgcg cacggtcggc gtcgcctgca
      721 ggtaggcgag catcgcggca taccggtcca tgggcttgcc gtcgaacttg agcttggcca
      781 cgacaggggt cgagttgccg acgaggtcca tgcgcgtgca gtgtccgcag tactgcgggc
      841 aggtcggcag catctcggcg agcaccttgg tcgggtagcg gtgggtcagc ccttcggcga
      901 cccacatgtc gtgctcgtgc agcgagtcgc gggtcgagtg cggatgcgag ggccagtcgc
      961 tgcgccggtc ggagaagacc gggagcatgt agcgccggat cgggtcggca tagaacgccg
     1021 cggtgaagtc ggtccccgct gtgggcatcg gctcgtccgt cgaggggacc atcgtgttca
     1081 tcatctgcgg cgtgacgagc atcgacatcg tcgcccgctc ggcctggtcg cgctccaggt
     1141 cggcgtagag ctcgtcggtg agcaggtcgc ccatgagctc gcgcagctgg cgaacgttct
     1201 tgacgcagtg ggaccgttgc cactgcacgt cggcccattc ggtggccgtg acgccgcggt
     1261 agcccggcag ccgggcccag tcgggctcga ccaggtcacg ctgctggtag tggtacggct
     1321 gctcgatcct tgcgctcata cctggcatga taacgaagga acgcccgaaa tcttgcatct
     1381 tggcgcaaga atctggcgta ccggtcagta accaacggag gatctatgag cccgtatgac
     1441 gagacggcac ctcgtgcagg tgcctcaccg atcggtctgc accgggttct cgagccgacc
     1501 ggcgacgccg tgaccctccc gcagatggcc cgccggatcg acccggaccc gcggatctgg
     1561 cccgacgagg tccgggtcga cgtcgacacg ctcaacctcg acgccgcgtc ctaccgccag
     1621 ctcgcgacca agcacacccg gcccgacggg accgtcgacg gcgacgccgt gcgggcggag
     1681 gtcctcgaca tcgtcgcgac gcgcgggaag atgcagaacc ccgtgaccgg ctcgggcggg
     1741 atgctgatcg gccgggtggc cgaggtcggc cccgactcga ccctggggct ggcgccgggt
     1801 gaccgcatcg ccactctcgt ctcgctgtca ctgacgcccc tgcagatcac cgacaggctg
     1861 gcgcgctggg acgggctggc ggagcaggtc cctacagcgg gcacggcgat cctcttcggg
     1921 cgcagcat
//
LOCUS       DATAIX010000569         2504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_193507, whole genome shotgun sequence.
ACCESSION   DATAIX010000569 DATAIX010000000
VERSION     DATAIX010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193507"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..718)
                     /locus_tag="VHM65_08425"
     CDS             complement(77..718)
                     /locus_tag="VHM65_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705771.1"
                     /translation="MIDGPTTARIVATRRQLHGIAECVMAGPQRRAGGLLTLRVTPGG
                     FATTGGPAVRLDGAELVIDEARRVPVAGDFHSVAEAVGVHFGPPPDGYSDGSGARAED
                     VLSLDLGAAELIAQWYLLSDAALRVLAPEQTPILWPEHFDVAILVDNTSIGSSPGDDF
                     HARPYAYVSSRDHGPAPYWNAPFGAVVSHEQIGGLDDLVGFWRQGQELLRAGG"
     gene            complement(885..>2504)
                     /locus_tag="VHM65_08430"
     CDS             complement(885..>2504)
                     /locus_tag="VHM65_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX2705772.1"
                     /translation="LSGDVTYALPDQSVLAPGGPHRARSAANDRVVESLTEVFDQFEI
                     DAQVTGFSRGPTVTRYEVELGPATKVERVTALSRNIAYAVASADVRILSPIPGKSAIG
                     IEIPNTDREKVSLGDVLRSQTARANEHPMVIGVGKDVEGGYVIANLARMPHLLVAGAT
                     GAGKQSFVNSMITSILMRATPEEVRMILVDPKRVELTAYDGIPHLITPIITNPKKAAE
                     ALQWVVREMDQRYDDLAAFGFKHLDDFNKAVRSGKVKTPPGSERVIAPYPYLLIIVDE
                     LADLMMVAPRDVEESIVRITQLARAAGIHLVLATQRPSVDVVTGLIKANVPSRMAFAT
                     SSLADSRVVLDQPGAEKLVGQGDALFLPMGVSKPMRVQGAWVTETEIHNVVKHVTAQL
                     KPVYREDVTVVAAKKHVEDDIGDDLELLLAATELVVTTQFGSTSMLQRKLRVGFAKAG
                     RLMDLLETRGIVGPTEGSKARDVLVKPDDLPITLSLLRGEDVPQSTAAGDHPPSAAPL
                     GTWTAPDQIPATAQDEEDGGSEDAWDLTGRG"
BASE COUNT          367 a          854 c          895 g          388 t
ORIGIN      
        1 acaactacgt cgacgatctg cacccgccgg gcaccgacgc cagcgatcca gacccatcgc
       61 gtcactagag ctgccgctag cctccggccc gcagcagctc ctgaccctgt cgccagaagc
      121 cgacgaggtc atcgagcccg ccgatctgct catggctgac gacggcaccg aaaggcgcat
      181 tccagtatgg ggcggggccg tggtcgcggc tgctgacgta ggcatagggg cgggcatgga
      241 agtcgtcacc cggtgaggag ccgatggagg tgttgtcgac gaggatggcg acatcgaagt
      301 gctcgggcca caggatcggg gtctgctcgg gggcgagaac gcgcagcgcg gcatcggaga
      361 ggagatacca ctgggcgatc agctccgccg ccccgaggtc gagcgagagg acatcctccg
      421 ctcgcgcgcc gctgccatcg gagtagccat ccgggggcgg cccgaagtgc acccccaccg
      481 cctcggcaac cgagtggaag tcgccggcca cgggcacccg gcgcgcctcg tcgatgacga
      541 gctccgcgcc gtcgagccgc acggcgggac cgcccgtcgt cgcgaaacca cccggagtca
      601 cgcgcagcgt cagcagccca ccggcgcgtc gctggggccc ggccatcacg cattccgcga
      661 tgccatgcag ctgacgccgg gtcgccacga tcctggccgt cgtcggtccg tcgatcatct
      721 cgtgctcctc ggggtgtaac gcggggggct gtccacagac actgctcttg aacgctgcgg
      781 acacgacagc caagcggtca tgccagtctg tgccacccgg acgaaagggc gtcccatcgc
      841 ggacgggtag tccgccacgc cctggcgcca cctgcccgcc acggctaccc tcggccggtc
      901 aggtcccagg cgtcctcgga cccgccgtcc tcctcgtcct gcgcggtcgc cggtatctgg
      961 tcaggcgcgg tccacgtgcc cagcggcgcg gccgacggcg ggtggtcacc cgccgccgtg
     1021 ctctggggca cgtcctcgcc acgcaggagc gacagcgtga tcggcaggtc gtccggcttg
     1081 acgagcacgt cacgcgcctt ggagccctcg gtcggtccga cgatgccgcg ggtctcgagc
     1141 aggtccatga gccggccggc cttggcaaag ccgacccgca gcttgcgctg gagcatcgag
     1201 gtcgagccga actgcgtcgt cacgaccagc tcggtggccg cgagcagcag ctcgaggtcg
     1261 tcgccgatgt cgtcctcgac gtgcttcttg gctgcgacga ccgtgacgtc ctcgcggtag
     1321 accggcttga gctgggccgt gacgtgcttg acgacgttgt ggatctcggt ctcggtgacc
     1381 cacgccccct ggacccgcat cggcttgctc acgcccatcg gcaggaagag cgcatcgccc
     1441 tggccgacga gcttctcggc acccggctgg tcgaggacga cccgggagtc ggccagcgag
     1501 gaggtcgcga acgccatccg cgagggcacg ttggccttga tgagcccggt gacgacgtcg
     1561 accgacggac gctgggtcgc gagcacgagg tgtatgccgg ccgcccgggc gagctgggtg
     1621 atccgcacga tcgactcctc gacgtcgcgc ggcgccacca tcatgaggtc ggccagctcg
     1681 tcgacgatga tgagcaggta ggggtagggc gcgatgacgc gttccgaccc gggcggcgtc
     1741 ttgaccttcc ccgagcggac cgccttgttg aagtcgtcga ggtgcttgaa gccgaacgcg
     1801 gcgaggtcgt cataccgctg gtccatctcg cggacgaccc actgcagggc ctcggcggcc
     1861 ttcttggggt tcgtgatgat cggggtgatg aggtgcggga tcccgtcata cgcggtgagc
     1921 tcgacccgct tggggtcgac gaggatcatc cggacctcct cgggcgtggc ccgcatgagg
     1981 atcgaggtga tcatcgagtt gacgaagctc tgcttgcccg cgccggtggc gccggcgacg
     2041 agcaggtggg gcatcctggc caggttggcg atgacgtagc cgccttcgac gtccttgccg
     2101 accccgatga ccatggggtg ctcgttggcc cgcgccgtct ggctgcgcag gacgtcgccg
     2161 aggctgacct tctcgcggtc ggtgttgggg atctcgatcc cgatcgcgga cttgccgggg
     2221 atcgggctca ggatgcgcac gtcggccgag gcgacggcat acgcgatgtt cctggacagc
     2281 gcggtgaccc gctcgacctt ggtcgccggg ccgagctcga cctcgtagcg cgtcaccgtc
     2341 ggtccccggc tgaacccggt gacctgcgcg tcgatctcga actggtcgaa gacctcggtc
     2401 agcgactcga cgacgcggtc gttggcggcg ctgcgggccc tgtgcgggcc gccgggggcg
     2461 aggaccgact ggtcgggcag tgcgtaggtg acgtcgccgg agag
//
LOCUS       DATAIX010000570         2288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_252117, whole genome shotgun sequence.
ACCESSION   DATAIX010000570 DATAIX010000000
VERSION     DATAIX010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252117"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1651)
                     /locus_tag="VHM65_08435"
     CDS             complement(<1..1651)
                     /locus_tag="VHM65_08435"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX2705773.1"
                     /translation="MIEIHDKGDTMAQVRQAVAARMTSRWGARVAVVLFVALAGGLLS
                     ATVTPTSSDPTAPSLPAEVESVRADSLAARLPSAGVNPAIIVVTRGGAALTPADLAAV
                     ATVGAELTSRYGAPPVAGAGGAEDGSSAGAAAGASPVIPSRDDEAALLTVSLPASLAG
                     EDLVSTVTAMRAVIESGLPDGLTAELTGGPGLATDISSVFEGADVRLLAVTASIVALL
                     LLVTYRSPLLWVVPLFVVGTADQLGAQLTKVVSAASGWEINESTSGIASVLVFGAGTN
                     YALLLIARYREELRRHADHRDAMREAMRSAAEPIVGSALTVTASLCTLALALDGGTRA
                     IGWTSALGVLVVLAGVVLVLPAALVLSGRRLFWPFIPWMGDPDPTEAGLWARIAGAVT
                     RRPHLVGITATVVLLVMASGLIGARVGLSQTEQFRIEAESVRADALAARLPSAEVNPA
                     IVIVTRGGAALTPADLAAVASVGAGLTGRYGAPLAAGAPVIPSSDGRAALLTVALPAA
                     LSGDELVSTVKELRAALGSALPDGLTAELTGGPGLATDTSSV"
     gene            1750..>2288
                     /locus_tag="VHM65_08440"
     CDS             1750..>2288
                     /locus_tag="VHM65_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2705774.1"
                     /translation="MASRDTKKRAQTVQEILALLRRVQVYGDRMASAFAALQGVHHTD
                     LTALIHIMDAQALGSPMTPSRLQHELRISSGATTAVIDRLERHGHAVRERDTVDRRKV
                     HLRHGDRARQVGADFFGPLGVLSDRVMGEFTDTQLETIRRFLALMAEAYAQHADTVDR
                     RQPGTGVTQQSHRKDMPPS"
BASE COUNT          373 a          828 c          819 g          268 t
ORIGIN      
        1 agaccgagct ggtgtcggtg gccagcccag gaccgccggt cagctcggcg gtgagcccgt
       61 ccgggagcgc ggagccgagc gcggcgcgca gctccttgac cgtgctcacc agctcgtccc
      121 cggacagtgc ggcaggaagc gcaacagtga gcaacgccgc ccgtccgtca ctggacggta
      181 tgacgggagc accagccgcg aggggcgcgc cataccggcc ggtcagcccc gcgccgaccg
      241 aggcgaccgc cgcgaggtca gcgggggtga gcgccgcacc gccacgggtc acgatgacga
      301 tggccgggtt gacctcggcg ctcggcagcc gggcggccag cgcgtcggca cgcaccgact
      361 cggcctcgat ccggaactgc tccgtctggg acaggccgac ccgcgcaccg atgaggccgc
      421 tggccatgac gagcagcacc acggttgccg tgatcccgac caggtgcgga cgccgggtca
      481 cggcgccggc gatgcgcgcc cagagtccgg cctcggtcgg gtcggggtcg cccatccagg
      541 ggatgaacgg ccagaacagg cggcggccac tcaggaccag agccgcgggg aggaccagga
      601 ccaccccggc aaggacgacg aggactccca gggcgctggt ccagccgatg gcccgggtcc
      661 cgccgtcgag cgcgagggcc agggtgcaca gcgaggccgt cacggtgagc gcactcccga
      721 cgatcggctc agcggccgag cgcatcgcct cgcgcatcgc gtcgcggtgg tcggcgtggc
      781 gccgcagctc ctcccggtag cgggcgatga gcagcagggc gtagttggtc cccgccccga
      841 agacgaggac gctggcaata cccgaggtcg actcgttgat ctcccacccg gaggccgccg
      901 acacgacctt ggtcagctgg gccccgagct ggtcagccgt cccgacgacg aagagcggca
      961 cgacccacag cagcggggag cggtacgtca cgagcaggag cagggcgacg atcgaggccg
     1021 tcaccgcgag caggcgcacg tcagcgccct cgaagaccga gctgatgtcg gtggccagcc
     1081 caggaccccc ggtcagctcg gcggtgagcc catcgggcag cccggactcg atgacggcgc
     1141 gcatcgccgt gaccgtgctg acgaggtctt cgccggccag ggatgcgggc agcgacaccg
     1201 tgagcagcgc cgcctcgtca tcacgagacg gtatgacagg ggatgcgccg gccgccgcac
     1261 ccgcggagct gccgtcctcg gcgccccccg cgccagccac ggggggcgcg ccataccggc
     1321 tggtcagctc ggcgccaacc gtggcgacgg cggcgaggtc tgcgggcgta agcgccgcgc
     1381 cgccacgcgt caccacgatg atggccgggt taacccccgc gctgggcagc cgggcggcca
     1441 gcgagtcggc gcgcaccgac tcgacctcgg ccggcagcga cggggccgtc gggtccgagc
     1501 tcgtcggcgt cacggtggcg ctgagcagac ctcccgccag ggccacgaag agcaccactg
     1561 cgacccgtgc tccccaccgg ctcgtcatcc gcgccgccac cgcctgccgg acctgagcca
     1621 ttgtgtcacc cttgtcgtgt atctcgatca tagagatact ctcttgagag gcatactagg
     1681 catatcgagg tgcgtgcggc aacgaggctg ccaccgaggc gaggcactgg agccggacag
     1741 gtcgggttgg tggcgagtcg ggacacgaag aagcgggcgc agacggtcca ggagatcctc
     1801 gcgctgctgc gacgcgtgca ggtgtatggc gaccgcatgg ccagcgcctt cgccgcgctg
     1861 cagggcgtcc accacactga cctcacggcc ctgatccaca tcatggacgc gcaggcgctc
     1921 ggcagcccga tgacccccag ccgtctccag cacgagctgc ggatctcctc aggggcgacc
     1981 acggcagtca tcgaccggct cgagcgccac ggccacgccg tgcgcgagcg cgacacggtc
     2041 gaccgtcgca aggtccacct gcgccacggt gaccgggcac gtcaggtggg tgccgatttc
     2101 ttcggtccgc tcggcgtgct cagcgaccgg gtcatgggtg agttcaccga cacccagctc
     2161 gagacgatcc ggcgcttcct ggcgctcatg gccgaggcgt acgcccagca tgccgacacg
     2221 gtcgatcggc gccagcccgg cacaggcgtc acacagcaaa gccacaggaa ggacatgccc
     2281 ccgtctca
//
LOCUS       DATAIX010000571         3113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_193737, whole genome shotgun sequence.
ACCESSION   DATAIX010000571 DATAIX010000000
VERSION     DATAIX010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193737"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(425..1720)
                     /locus_tag="VHM65_08445"
     CDS             complement(425..1720)
                     /locus_tag="VHM65_08445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2705775.1"
                     /translation="MTMTVAAAVERLFDNTAIRLHAYDGSVTGPPDGLLVQLVSERGL
                     NYLLTGPGSLGLVRAYLMGDLVFEGITEGDPHDVLRLLDDGFHGRLPSMSDAPALARF
                     VAAHRPWMPELPPEETPGRVRRLAYGLAHGRRRDAEAISHHYDVSNRFYEMLLGPSMA
                     YTCAAYPRAESPLDEAQEHKFDLVCRKLGLAPGMRLLDVGCGWGGMVRHAVTHYGVTA
                     LGVTLSSQQARYAADRLAADGLRGRAEIRHVDYRDVREGGFDAVSSIGLTEHIGPRNY
                     PSYFQFLHAKLAPGGRLLNHCITRPDNEHAGLPERGFINRYVFPDGGLTGSGDLVRAA
                     ENAGFEVRHHENLREHYALTLRDWCSNLSKHWDEAVMEAGLGVARVWGLYLAGSRLSF
                     ERNRIQLHQVLMARTTDDGVSGFGLPVLWPSGDEGVRQR"
     gene            complement(1717..>3113)
                     /locus_tag="VHM65_08450"
     CDS             complement(1717..>3113)
                     /locus_tag="VHM65_08450"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2705776.1"
                     /translation="HAQAVTEVARQFAGLDPDTPVRLAKRTSNLFRPRAAPSAPGLDV
                     SGLDSVVEVDVVARTATVQGMATYEHVVDATLAHGLMPLVVPQLKTITVGGAVTGLGI
                     ESSSFRNGLPHESVRAMEVLTGSGEVVTAYPQGPYADLYRAFPNSYGSLGYAMSLVIE
                     LEPVQPYVRLRHVRFSGLGPLVEAIGRVMTAHAYEGTPVDFIDGVVFGRHEAYLTLGT
                     WAGGLDAAERPSDYTGQDIYYRSIQRLGRDVLTVRDYLWRWDTDWFWCSRAFGAQHPL
                     LRRVWPRQWLRSDVYSTIVGVENRHGWAARVDRLRGRPARERVVQDVELALDRTADFL
                     DWFLAEVPIEPVWLCPVQLRTEWPRAAGVGDERTEPPWPLYPMAPSVPYVNVGFWSTV
                     PVAPGHADGDVNREIERVVGELGGHKSLYSDSYYGREAFLAKYGGTTYAKVKRRYDPQ
                     GRFPELYDKAVRGR"
BASE COUNT          495 a         1101 c         1079 g          438 t
ORIGIN      
        1 tccgcccatt cggcctggat gagcggctat tgcccgactt cgagcgacgg aggaatgtcg
       61 accctgactc ccctcggaca ccttcgcccg tcacctagcg atcacggccg gggaactgga
      121 ccgagaggcc tgcggcaggg ctggccctcg gcggccccgt cgaggcggct ggcgagggtg
      181 aggtgcggga acggatcacc gaggcgctcg cgggcggacg gctgatcggc agctgaccac
      241 tccgcgccgg gatggtggcg gcatgtgttc ggacgctcga cggcggcctg gcgccaccag
      301 cgcagcccgc gtatcgagga ggtgaggcgc ctgcggctcg aggccccggg gcgggcgtcg
      361 tcagcaagta cgacaaagca ggaggtggcc aatgcggtaa caccgccgcc aaggagatcg
      421 ccacctaccg ctgacggaca ccctcgtcgc cgctcggcca caacaccggc agcccgaacc
      481 cggacacccc gtcatcggtc gtacgcgcca tcagcacctg gtgcagctgg atccggttgc
      541 gctcgaagga cagccgggac ccggcgagat agagacccca cacccgcgcg acgcccaggc
      601 cggcttccat cacggcctcg tcccagtgct tcgacaggtt ggagcaccag tcacgcaggg
      661 tcagcgcata gtgctcacgc aggttctcgt gatggcgcac ctcgaacccg gcgttctcgg
      721 ccgcgcgcac gaggtccccc gacccggtga gccccccgtc gggaaagacg tagcggttga
      781 tgaacccccg ctccggcaga cctgcgtgct cgttgtccgg ccgggtgatg cagtggttga
      841 gcagccgccc gcccggagcg agcttggcgt gcaggaactg gaagtacgac gggtagttgc
      901 gcgggccgat gtgctcggtc agcccgatcg agctcaccgc gtcgaagccg ccctcgcgca
      961 cgtcccggta gtccacatgc cggatctcgg cccgcccccg aagcccgtcc gcagcaaggc
     1021 gatccgcggc ataccgggcc tgctgagatg agagcgtgac gccgagtgcc gtgaccccgt
     1081 agtgggtgac cgcatgccgg accatcccgc cccagccgca gccgacatcg agcaggcgca
     1141 tccccggggc tagtccgagc ttgcggcaga cgaggtcgaa cttgtgctcc tgcgcttcgt
     1201 cgagcgggga ctcggctcgg gggtaggccg cgcaggtgta ggccatcgac gggccgagca
     1261 gcatctcgta gaagcggttc gacacgtcgt agtggtgcga gatggcctcg gcgtcgcggc
     1321 ggcgcccgtg agccagaccg tacgcgagcc gacgcacccg ccccggcgtc tcttcgggtg
     1381 gtagctcggg catccagggc cgatgtgccg cgacgaacct ggcgagcgcc ggagcgtcgg
     1441 acatcgatgg cagccgcccg tgaaacccgt catcgagcaa cctgagcacg tcgtgggggt
     1501 cgccttcggt gatgccctcg aagacgaggt cgcccatgag ataggcccgg accaggccga
     1561 gcgaccccgg cccggtcagc aggtagttga gcccgcgctc gctcaccagc tgcacgagca
     1621 ggccgtccgg cggaccggtc accgacccgt catacgcgtg gagccggatc gccgtgttgt
     1681 cgaagagccg ctccaccgcg gccgccacgg tcatcgtcat ctgcccctca ccgccttgtc
     1741 gtagagctcc gggaagcgcc cctgcgggtc atagcggcgc ttgaccttcg cgtatgtcgt
     1801 cccgccgtac ttcgcgagga aagcctcccg cccatagtaa gagtcggagt agagggactt
     1861 gtgtccaccg agctcgccga cgacgcgctc gatctcgcgg ttgacgtcgc cgtcggcgtg
     1921 cccgggtgca accggcaccg tcgaccagaa gccgacgttg acatagggca cgctcggcgc
     1981 catgggatag agcggccagg gcggttcggt ccgctcgtcg ccaacccccg cagcccgggg
     2041 ccactccgtc cggagctgca ccgggcacag ccagaccggc tcgatcggca cctcagcgag
     2101 gaaccagtcg aggaagtctg cggtccggtc gagtgccagc tcgacatcct ggacgacccg
     2161 ctcgcgcgcc ggccggccac gcaggcggtc cacccgtgcc gcccacccgt gccggttctc
     2221 gacaccgacg atggtcgagt agacgtcgct gcgcagccat tgccggggcc acacccggcg
     2281 cagcagtgga tgctgcgccc cgaacgcccg cgagcaccag aaccagtcgg tgtcccagcg
     2341 ccacaggtag tcgcgcaccg tgaggacatc gcgcccgagc cgctggatcg agcggtagta
     2401 gatgtcctgg ccggtgtagt cgctcgggcg ctccgccgcg tcgagccctc ccgcccaggt
     2461 cccgagggtg agataggcct cgtgccggcc gaagaccacg ccgtcgatga agtcgacggg
     2521 cgttccctca taggcgtggg cggtcatgac ccgcccgatc gcctcgacga gcgggccaag
     2581 gcccgagaac cgcacatggc gcagccggac gtacggctgg acgggttcga gctcgatgac
     2641 cagtgacatg gcgtagccga gcgagccgta ggagttcggg aacgcccggt acaggtcggc
     2701 gtacggtcct tgggggtatg ccgtcaccac ctcgccgctg ccggtcagga cctccatggc
     2761 ccggaccgac tcgtgcggca ggccgttgcg gaacgacgag gactcgatcc ccagcccggt
     2821 cacggctccc ccgacggtga tcgtcttgag ctggggcacg acgagcggca tgagcccgtg
     2881 cgccagggtt gcgtcgacga cgtgctcgta cgtggccatc ccctgcaccg tcgcggtccg
     2941 ggcgacgaca tccacctcga ccaccgagtc cagcccgctg acgtccagcc ccggcgccga
     3001 tggcgcggcc cgtgggcgga agaggttcga ggtgcgcttg gccagtcgca cgggcgtgtc
     3061 cgggtccagt ccggcgaact gtcgggccac ctcggtcacg gcttgggcat gat
//
LOCUS       DATAIX010000572         2957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_282368, whole genome shotgun sequence.
ACCESSION   DATAIX010000572 DATAIX010000000
VERSION     DATAIX010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282368"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VHM65_08455"
     CDS             complement(<1..294)
                     /locus_tag="VHM65_08455"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF016438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX2705777.1"
                     /translation="MSVSLGMPGVPQAVVAPRRVSRKIRVGTVEVGGDAPVSVQSMTT
                     TPTTDVDATLQQIAELTATGCDIVRVACPTQDDADALAAIAKHSQLPVIADIHF"
     gene            complement(335..1684)
                     /locus_tag="VHM65_08460"
     CDS             complement(335..1684)
                     /locus_tag="VHM65_08460"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX2705778.1"
                     /translation="MLYLLGVLVVVVGVGLSIALHEIGHLVPAKRFGVKVSQYMVGFG
                     PTVWSRTRGETEYGVKAVPLGGYVRMIGMFPPAQGADPGLLRSSSTGRFSQLMDQARQ
                     DSLEQVEPGDEDRVFYRLSVPRKVVVMLGGPLMNLLIAAVLITGIITLYGVPVAKDGA
                     VIASVSECVVPAAQASTTTTCAPTDPKTPAYLAGLKAGDKVVSVNGVTLRNSGDLGAQ
                     IQPRTGQPTTVLVERAGRLLSLTVTPVKNTVGTYDDQGQPVLDASGRPVVVEAGFLGV
                     RSSSTVVLERQPVTAAPGAVWSAVAQTASVVVRVPEKMVGITQAAFGSGERDPNGPIS
                     VVGVGRVGGEIASADLGEDGTLAKLITLLWLIASLNLALFVFNLIPLLPLDGGHVLGA
                     LWEGVKRQVARLRGRPDPGYVDVAKALPVAYAVSMVLIVMSGLLIYADIVKPIKLFG"
     gene            complement(1684..2889)
                     /gene="dxr"
                     /locus_tag="VHM65_08465"
     CDS             complement(1684..2889)
                     /gene="dxr"
                     /locus_tag="VHM65_08465"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX2705779.1"
                     /translation="MTTRTVTLLGSTGSIGTQAIDIVRANPARFTVHALTAGGAQLNL
                     LARQAVELGVEAVGTAAGDPGRLREALAHHAREAGRPSYRPEVLAGPDASTTVAARPV
                     DVVLNGITGAIGLAPTLAALRAGSILALANKESLIVGGPVVTAAARPGQIVPVDSEHS
                     ALAQCLRGGAAEEVSRLVVTASGGPFRGMARGQLHDVTPQQALAHPTWAMGPMVTTNS
                     ATLVNKGLEVIEAHLLFGVPFERIEVVVHPQSVVHSMVEFTDGSTLAQASPPDMRLPI
                     ALGLDWPRRVPGAAHPCDWRAAATWEFFPLDDEAFPAVRLAKEVGAAGGTFPAVYNAA
                     NEECVQAFWAGRIRFTDIVDTVARVVAAHDGIDLAHLDVEAVLGADAWARAEARAVLG
                     LEPAHGKGS"
BASE COUNT          425 a         1049 c         1082 g          401 t
ORIGIN      
        1 gaagtggata tcggcgatga ccgggagctg gctgtgcttg gcgatggccg cgagcgcgtc
       61 ggcgtcgtcc tgggtcgggc aggccacccg gacgatgtcg cagccggtgg ccgtgagctc
      121 ggcgatctgc tgcagcgtcg cgtcgacgtc ggtcgtcggc gtcgtcgtca tcgactggac
      181 cgagacgggg gcgtcgccgc cgacctcgac cgttccgacc ctgatcttgc gggagacccg
      241 gcggggggcg acaacggcct ggggcacccc aggcataccg agggagacac tcatgagcgc
      301 cagtatcctc gccctgctgc gcagtgcctc tcgctcaccc gaagagcttg atcggcttga
      361 cgatgtcggc gtagatgagc aggcccgaca tgacgatgag gaccatggag acggcgtagg
      421 ccacgggcag ggccttggcc acgtcgacgt accccgggtc gggccgtccc cgcagccgtg
      481 ccacctgacg cttgaccccc tcccataggg cgccgaggac gtggccgccg tcgagcggga
      541 gcagcgggat gaggttgaag acgaacagcg ccaggttgag cgaggcgatg agccagagca
      601 gcgtgatgag cttggccagg gtcccgtcct cgccgaggtc ggcgctggcg atctcccccc
      661 cgacccggcc gacgccgaca acggagatcg gcccgttggg gtcgcgctcg ccggacccga
      721 acgccgcctg ggtgatgccg accatcttct cgggcacccg gaccacgaca ctggccgtct
      781 gggcgaccgc gctccagacc gcccctggtg cggcggtcac cggctggcgc tcgagaacca
      841 ccgtcgagga cgagcgcacc ccgaggaagc cggcctcgac gacgacgggc cggcccgagg
      901 cgtcgagcac gggctggccc tggtcgtcgt aggtgccgac ggtgttcttg accggggtca
      961 ccgtcaggct cagcagccgt cctgcgcgtt cgacgagcac ggtggtcggc tgacccgtgc
     1021 ggggctggat ctgggcgccg aggtccccgc tgttgcgcag ggtgaccccg ttgaccgaca
     1081 cgaccttgtc gccggccttg agcccggcca ggtatgccgg ggtcttgggg tcggtcggcg
     1141 cacacgtggt cgtggtgctg gcctgtgcgg ccggcacgac gcactcgctg accgaggcga
     1201 tgacggcgcc gtccttggcg accgggacgc catacaaggt gatgatcccc gtgatgagca
     1261 ccgccgcgat gagcaggttc atgagcgggc cgccgagcat gacgacgacc ttccggggca
     1321 ccgagagccg gtagaagacc cggtcctcgt cgcccggttc gacctgctcc aacgagtcct
     1381 gccgggcctg gtccatgagc tggctgaacc ggccggtcga ggaggagcgc agcaggcccg
     1441 ggtcggcccc ctgggccggg gggaacatgc cgatcatccg gacgtagccc ccgagcggga
     1501 cggccttgac gccgtactcg gtctcgccgc gggtgcgcga ccagaccgtg gggccgaagc
     1561 cgaccatgta ctggctcacc ttgacgccga accgcttggc cgggacgagg tgcccgatct
     1621 cgtgcagggc gatcgagagc cccaccccca cgacgacgac gagcactccc agcaggtaga
     1681 gcatcacgat ccctttccgt gggcgggctc cagcccgagg acggcacgtg cctcggcgcg
     1741 ggcccacgcg tcggcaccca gcaccgcctc aacgtccagg tgggccaaat cgatcccatc
     1801 gtgggcggcc acgacccggg ccaccgtgtc gacgatgtcg gtgaagcgga tccggccggc
     1861 ccagaaggcc tgcacgcact cctcgttcgc cgcgttgtag acggccggga aggtcccgcc
     1921 cgccgcaccg acctccttgg ccaggcgcac cgccgggaac gcctcgtcgt cgagcgggaa
     1981 gaactcccac gtggcggcgg cccgccagtc gcacgggtgg gccgctcccg ggacccggcg
     2041 cggccagtcg aggccgagcg cgatgggcag gcgcatgtcg ggcggcgatg cctgggccag
     2101 cgtcgacccg tcggtgaact cgaccatcga gtgcacgacc gactggggat ggacgacgac
     2161 ctcgatgcgc tcgaacggga cgccgaagag caggtgcgcc tcgatcacct ccagcccctt
     2221 gttgaccagg gtggctgagt tcgtcgtcac catcgggccc atggcccagg tcgggtgggc
     2281 cagcgcctgc tgcggcgtga cgtcgtggag ctgcccgcgc gccatccctc tgaacgggcc
     2341 tccgctggcc gtcacgacca accgcgacac ctcctcggcc gctcccccgc gcaggcactg
     2401 ggccagggcg ctgtgctcgg agtcgaccgg cacgatctgg ccgggccggg cggccgccgt
     2461 gaccaccggc ccgccgacga tgagggactc cttgttggcc agggccagga tgctgcccgc
     2521 ccgcagtgcg gccagggtgg gcgccagacc gatcgccccg gtgatgccgt tgaggacgac
     2581 gtcgacgggc cgggcggcga ccgtggtgct ggcatcgggt ccggccagca cctccggccg
     2641 gtatgacggg cgccccgcct cgcgcgcgtg gtgggccagg gcttcgcgca gccggccggg
     2701 gtcacccgcg gcagtcccga cagcctcgac gccgagctcg accgcctggc gggccagcag
     2761 gttgagctgg gctccgccgg ccgtgagggc gtgcacggtg aaccgcgccg ggttggcccg
     2821 gacgatgtcg atggcctggg ttccgatcga ccccgtcgag ccgagcaggg tcacggtgcg
     2881 cgtcgtcact ggcccattgt cgtccacgcg tccagtgccg ccccgagacc ccgggagggg
     2941 aacagcccgc ctcacgc
//
LOCUS       DATAIX010000573         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_193796, whole genome shotgun sequence.
ACCESSION   DATAIX010000573 DATAIX010000000
VERSION     DATAIX010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193796"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VHM65_08470"
     CDS             complement(<1..888)
                     /locus_tag="VHM65_08470"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX2705780.1"
                     /translation="MSNVPWLSIIGAVPLVGAVVVVLLPNRPSVPAKAVALGFSLLTL
                     LVTILAATQFKTSSVTQFQLVENHPWIPQFGVSYALGVDGIALTLILMSVILTPVCLL
                     AAWHDVPDESAQRQQNYLALMLVLETFMVLVFSATDVFLFYVLFEGMLIPVYFLIGSY
                     GGARRQYASMKFLIFSLFGGLVMLVAVIGLFVQGPGGDDGFLVERLTGLAMDPTTERL
                     LFVGFFFAFAVKAPMWPVHTWLPDAATEAPPATSVLLVGVLDKVGTYGMIRFCLQFFP
                     EASRWATPVVIALAVISILY"
     gene            complement(888..>2000)
                     /locus_tag="VHM65_08475"
     CDS             complement(888..>2000)
                     /locus_tag="VHM65_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX2705781.1"
                     /translation="MVTAGVYLVVRSHAIYDAAPNAQLAVVIVGAVTLLFGAVVGCAK
                     DDIKKALAASTMSQIGYMMLAAGLGPVGYAFAIFHLLTHGFFKAGMFLGAGSVMHGMN
                     DQVDMRRFGAVSTVMRTTWITFGLGWLAILGVPPFSGFWSKDKIIEAAFVGEGWRPWL
                     FGLVTMAGAGITAFYMSRLFFMTFHGSKRWANSVHPHESSAVMTVPMIILAVGSALLG
                     AVLSIGGMFTSWLEPVVGAHPEGAHPVLAVPAITGLTLALVAGGVWVAWAGYGRAVVP
                     QTAPAGSLLTRAARQDLYQDQVNEGLFMRPGIHLTRSLVFVDNRGVDGAAGGLAAFVG
                     GLSAQLRKVQNGYVRSYALTMLTGVVVILGAMWVIQ"
BASE COUNT          394 a          654 c          707 g          245 t
ORIGIN      
        1 atacaggatc gagatcacgg cgagcgcgat gacgacgggc gtggcccacc tgctggcctc
       61 ggggaagaac tgcaggcaga agcggatcat cccgtaggtg ccgaccttgt ccagcacgcc
      121 gaccagcagg accgacgtcg ccgggggcgc ctcggtcgcg gcgtcgggca gccaggtgtg
      181 gaccggccac atcggcgcct tgacggcgaa cgcaaaaaag aagcccacga agagcagccg
      241 ctcggtcgtc gggtccatcg ccagaccggt gagccgctcg acgaggaacc catcgtcccc
      301 gcctgggccc tggacgaaca gcccgatgac cgcgacgagc atgacgaggc cgccgaacag
      361 cgagaagatc aggaacttca tcgaggcgta ctgccggcgc gcgccgccat aggagccgat
      421 gaggaagtag accgggatga gcatgccctc gaagagcacg tagaagagga acacatccgt
      481 cgccgagaag acgagcacca tgaacgtctc gaggacgagc atgagggcga ggtagttctg
      541 ctggcgctgg gccgactcgt ccgggacgtc atgccaggca gcgaggaggc acaccggcgt
      601 gaggatgacc gacatgagga tgagcgtcag ggcgatgccg tcgacaccga gggcgtagct
      661 caccccgaac tgcgggatcc acgggtggtt ctcgacgagc tggaactggg tcaccgagct
      721 ggtcttgaac tgcgtcgcgg ccaggatcgt cacgagcagc gtgagcagcg agaaaccgag
      781 cgcgaccgcc ttggccggca ccgacgggcg gttcgggagg agcacgacga cgacggcccc
      841 gacgagcggg accgcgccga tgatgctcag ccaggggacg ttggacatca ctggatcacc
      901 cacatggcgc cgaggatcac gacgacgccg gtgagcatcg tcagtgcgta ggaccggacg
      961 tagccgttct ggaccttgcg cagctgcgcg gacaggcctc cgacgaaggc ggccagcccc
     1021 ccggcggccc cgtcgacgcc gcggttgtcg acgaagacga gcgatcgggt gagatggata
     1081 ccgggtcgca tgaacagccc ttcgttgacc tggtcctggt agaggtcctg gcgggcggcc
     1141 cgggtgagca ggctcccggc cggggccgtc tgcgggacca ccgcacggcc atagccggcc
     1201 cacgcgaccc acacgccgcc ggcgacgagc gccagcgtga gccccgtgat ggccgggacc
     1261 gcgagcacgg ggtgcgcgcc ctcagggtgg gctcccacga cgggctcgag ccaggaggtg
     1321 aacatcccac cgatcgacag gaccgccccg agcagggccg acccgacggc aaggatgatc
     1381 atggggacgg tcatcaccgc cgacgactcg tgcgggtgga cactattggc ccagcgcttg
     1441 ctcccgtgga aggtcatgaa gaacagccgc gacatgtaga acgcggtgat cccggcgccg
     1501 gccatcgtga ccaggccgaa gagccagggc cgccagccct caccgacgaa ggccgcctcg
     1561 atgatcttgt ccttgctcca gaacccggag aacggcggca ccccgaggat ggccagccag
     1621 cccaggccga aggtgatcca cgtcgtcctc atgacggtcg agaccgcacc gaagcggcgc
     1681 atgtcgacct ggtcgttcat cccgtgcatg accgagcccg cgccgaggaa catcccggcc
     1741 ttgaagaacc cgtgggtcag gaggtggaag atcgcgaacg cgtagccgac cggaccgagc
     1801 ccggccgcga gcatcatgta gccgatctgg ctcatcgtcg acgcggcgag cgccttcttg
     1861 atgtcgtcct tggcgcagcc gacgaccgcc ccgaagagga gggtcaccgc gccgacgatg
     1921 accacggcga gctgggcgtt gggggccgcg tcgtagatcg cgtggctgcg cacgacgagg
     1981 tagacaccgg ccgtcaccat
//
LOCUS       DATAIX010000574         2863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_252710, whole genome shotgun sequence.
ACCESSION   DATAIX010000574 DATAIX010000000
VERSION     DATAIX010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252710"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2822)
                     /gene="dnaE"
                     /locus_tag="VHM65_08480"
     CDS             complement(<1..2822)
                     /gene="dnaE"
                     /locus_tag="VHM65_08480"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX2705782.1"
                     /translation="MLARGSRDGLAHGTGWATLCRLVTATHLGGERGAPMTSRELVAD
                     HAHLRDGTGTGRDGGDLSRLIEARAHGGGGAGRDEPSSLVVLLGPDSDVGRAVLAKRA
                     EVARAALARWRALLPRDGLVIEVVCHGGPEGTPASLGQAARMLALAREAGVPAVLTAA
                     VRHADPGDAVTVDILDAARRLVPLDVRHLDRVTSAAYLAPTAAMRGIALDVVRASGPG
                     ISGGGVLAREGADQLMRTTDELAAICVQDPGRDLGIGSVHLPEPEAIGLRPGEDPQHV
                     LVQRCRGALATRYPHASPGDLAAVTSRLEQELGVIAGMGYPSYFLTVATVCDLIKDMG
                     VRVAARGSGAGSLVNFLLGISGVDPMRYGLLMERFCSPLRAQLPDIDIDVESARRTEI
                     YEQVLARFGGERVTCVSMMDTYRVRHAIRDVGATLGLAPVEIDAIAKAFPHIRARDAR
                     AAIAELPELRASGLGAPRLRVLFDLVERLDGLPRHIAVHPCGVILSNSGLLDRTPVEA
                     SWLGFPMSQFDKDDVETLGLLKLDILGIRMQSAMAHAVAEVRRVDGPEVDLDDRAQVP
                     LDDEATFRLIRTTHTLGCFQIESPGQRELIGKFGPERFEDLIIDISLFRPGPVKSDMI
                     VPFLNSRHGWSQAEYLHPTLIPALRETEGVVVFHEQVLLIVAETAGVTLAEADEVRRA
                     LGSVQGQREVEAWWRPAARARGYEAQVADRIWEVLKAFASFGFCKAHAAAFALPTYQS
                     AWLKTHHPAAFLAGVLTHDPGMYPKRLILDDARSLGIAVLGLDVNASTGDYHIERVDE
                     PSHHVPAHPDLPDGSRHGIRLSLADVLGMSEAETARIVAGQPYASLPDFWVRAQVSRP
                     VVERLVLAGGFDSLYGIGSITLAGGELGRRGRITRRDLLLHAAELDRWSRSVSRGRGG
                     GGRGRGRSGPSALRVTAGGGSTGSSGA"
BASE COUNT          400 a         1012 c         1093 g          358 t
ORIGIN      
        1 gccccggacg accctgtcga ccccccgccc gcagtgacac ggagggcgga tgggccgctg
       61 cggcctcgcc cccgaccacc gccgccccgg ccccggctga ccgagcggct ccaccggtcg
      121 agctcggcgg catgcaggag caggtcccgc cgggtgatcc ggccgcgccg gccgagctcc
      181 ccgccggcca gcgtgatcga cccgatgccg tagagcgagt cgaacccccc ggcgaggacg
      241 agccgctcga cgaccggccg ggacacctgt gcccgcaccc agaagtcggg caggctcgca
      301 tagggctgtc cggcgacgat ccgggcggtc tcggcctcgc tcatcccgag gacgtcggcc
      361 agggagagcc ggatgccgtg gcggctgccg tcaggcaggt cggggtgggc cgggacgtgg
      421 tgcgagggct cgtcgacccg ctcgatgtgg tagtcccccg tcgaggcgtt gacgtcgagg
      481 ccgagcacgg cgatcccgag gctgcgggcg tcgtcgagga tgagccgctt gggatacatg
      541 ccggggtcgt gggtgaggac tccggcgagg aaggccgccg ggtggtgggt cttgagccag
      601 gccgactggt atgtcgggag cgcgaaggcc gccgcgtggg ccttgcagaa cccgaacgac
      661 gcgaacgcct tgaggacctc ccagatccgg tcggcgacct gcgcttcata ccctctggct
      721 cgtgcggcgg gccgccacca ggcctcgacc tctcgctgac cttgaaccga gccgagggcc
      781 cggcggacct cgtcggcctc ggcgagggtg accccggccg tctcggcgac gatgagcagg
      841 acctgctcgt ggaagacgac gaccccctcg gtctcgcgca gggcggggat gagcgtgggg
      901 tggaggtact ccgcctggga ccagccgtgc cgggagttga ggaagggcac gatcatgtcg
      961 gacttgaccg ggccgggccg gaacagcgag atgtcgatga tgaggtcctc gaagcgctcc
     1021 ggcccgaact tgccgatgag ctcgcgctga ccgggactct cgatctggaa gcagccgagg
     1081 gtgtgggtcg tgcggatgag ccggaaggtc gcctcgtcgt cgagcggcac ctgggcgcgg
     1141 tcgtcgaggt cgacctcggg cccgtcgacc cggcggacct cggccacggc gtgggccatc
     1201 gccgactgca tgcggatgcc gaggatgtcg agcttgagca gcccgagggt ctcgacgtcg
     1261 tccttgtcga actggctcat cgggaagccg agccaggagg cctcgaccgg cgtacggtcg
     1321 agcaggccgg agttggacag gatgaccccg cacgggtgga ccgcgatgtg ccggggcagc
     1381 ccgtcgaggc gctcgacgag gtcgaagagc acccgcaggc gtggtgcgcc gagcccgctc
     1441 gcccgcagct cgggcagctc ggcgatcgcg gcgcgggcgt cgcgggcccg gatgtgcggg
     1501 aacgccttgg cgatcgcgtc gatctcgacc ggcgccagcc cgagggtggc cccgacgtcg
     1561 cggatcgcgt ggcggacccg gtaggtgtcc atcatcgaca cgcaggtcac ccgctccccg
     1621 ccgaagcggg cgaggacctg ctcgtagatc tcggtgcgcc gggccgactc gacgtcgatg
     1681 tcgatgtcgg gcagctgggc ccgcagcggc gagcagaacc gctccatgag cagtccgtag
     1741 cgcatggggt cgaccccgga gatcccgagc aggaagttga cgagcgaccc ggcccccgac
     1801 ccgcgcgcgg ccacccgcac acccatgtcc ttgatgaggt cgcagactgt cgcgacggtg
     1861 aggaagtatg acggataccc catgcccgcg atgaccccca gctcctgctc gagccggctg
     1921 gtgacggcgg ccaggtcacc ggggctcgcg tgcgggtagc gcgtcgcgag cgcgccgcgg
     1981 catcgctgga ccaggacgtg ctgggggtcc tcgcctgggc gcaggccgat cgcttcgggc
     2041 tcgggcaggt ggaccgaccc gatgccgagg tcacggcccg ggtcctggac gcagatggcg
     2101 gccagttcgt ccgtcgtccg catcagctgg tcagcgccct cccgggccag gactccccca
     2161 ccgctgatgc ctgggcccga ggcccggacg acgtcgaggg cgatgccacg catcgccgca
     2221 gtcggggcga ggtaggccgc gctcgtcacc cggtcgaggt ggcgcacgtc gagggggacg
     2281 agccgacggg cggcgtcgag gatgtcgacg gtgaccgcgt cgccggggtc ggcatggcgg
     2341 acggcggccg tgagcacggc cgggacgccc gcctcccggg ccagggcgag catccgggcc
     2401 gcctgcccga ggctggccgg ggtgccttca gggccgccat gacagacgac ctcgatgacg
     2461 agaccgtccc gcggcagcag ggcacgccag cgggccagcg ccgcccgggc cacctcggcc
     2521 cgcttggcca gcaccgcccg cccgacatcg gagtccggcc cgaggagcac cacgagcgag
     2581 gacggctcgt cccggccggc accgccgccg ccatgggcac gggcttcgat gagccgggac
     2641 aggtcgccgc cgtcccggcc cgtgccggtc ccgtcccgaa ggtgggcgtg gtcggcgacg
     2701 agctcacggc tcgtcatcgg cgcgccccgc tcgccgccca ggtgagtcgc cgtgaccagc
     2761 cggcagagcg ttgcccagcc cgtgccatgg gcaagcccgt cgcggctgcc gcgcgcgagc
     2821 acgacgactc tcgggtgacg tgggtcgacg atcgccccac cgc
//
LOCUS       DATAIX010000575         3473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_133681, whole genome shotgun sequence.
ACCESSION   DATAIX010000575 DATAIX010000000
VERSION     DATAIX010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133681"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VHM65_08485"
     CDS             <1..529
                     /locus_tag="VHM65_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705783.1"
                     /translation="FTFYEIDPVVVRVAKDSTMFTFLSDMPTPASIVLGDARLSLRTV
                     PDGTHDILVMDAYSSDSPPAHLLTVEAIGDAMRTVVSGGLLIFHVSNRYYDLAPVVAG
                     AAEALGLASLQRAYAPSTTDVERLVATPSIWVVLARRPADLAALASRGWTSITPVPPL
                     TDDNPDILRLLRLWR"
     gene            583..1800
                     /locus_tag="VHM65_08490"
     CDS             583..1800
                     /locus_tag="VHM65_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HEX2705784.1"
                     /translation="MIDEGAIRTRYGALSAALDERSRRLFLGAEALAAGRGGQAAVTR
                     ATGASPATIRRGLVELGAPGQLASGRIRRPGGGRKRTVELDPTLRADLEALIEPGTRG
                     DPESPLRWTTKSVRRLAAELGRGGHRVSYRIVAGLLHELGYSLQANRKVLEGTHHPDR
                     NAQFEHLNAAVQLQLSLGQPVISVDTKKKELVGPFRNAGRELRPKGDPERVLMHDFVI
                     DELGRASPYGVYDVAANEAWVSVGTDHDTAAFAVESIRRWWATMGQELYPDAKQLLVT
                     ADAGGSNGYRLRLWKLELQKLADETGLEIAVCHFPPGTSKWNKIEHRLFSAISQNWRG
                     KPLVSHEVIVKLIAATTTRTGLRVRSALDENHYPAGRTVSDADMDTLHLRPDAFHGEW
                     NYSLLPRLMLPEG"
     gene            1867..3099
                     /locus_tag="VHM65_08495"
     CDS             1867..3099
                     /locus_tag="VHM65_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HEX2705785.1"
                     /translation="MIDEAAIGARFLAVASLFDERARRLLAGSEALAIGRGGVAAVAR
                     ATGLARTTVARGVADVQAGETVALGRIRRAGAGRPPIEKRDPTLRADLEALIEPTSRG
                     DPESPLRWTTRSVRNLAAELRRQGHAVSHQTISELLHDLGYSLQANRKTLEGTSHPDR
                     DAQFRHINEAVQLQLALGEPVISVDTKKKELVGPFRNGGRELRPKGDPERVLMHDFVI
                     PELGRASPYGVYDVAANEAWVSVGTDHDTAAFAVESIRRWWTTMGAPLYPEATRLLVT
                     ADAGGSNGYRLRLWKQELGKLADEIGLEIAVCHFPPGTSKWNKIEHRLFSAITQNWRG
                     KPLVSHETVVNLIAATTTRTGLRVRSELDTSDYPAGLRVSDADMETLYLRRDDFHGEW
                     NYSLLPRQRLSLTEAVVS"
BASE COUNT          549 a         1252 c         1173 g          499 t
ORIGIN      
        1 cttcacgttc tacgagatcg acccggtggt cgtgcgggtc gccaaagact cgaccatgtt
       61 cacgttcctc tccgacatgc cgacgccggc ctcgatcgtg ctcggcgacg cgcgcctctc
      121 gctccgcacc gtgcccgacg gcacgcacga catcctcgtg atggacgcct actcgtccga
      181 ctcgccgccg gcccatctcc tgacggtcga ggcgatcggc gacgccatgc gcacggtcgt
      241 atcaggcggc ctcctcattt tccacgtgtc gaaccgctac tacgacctcg cgcctgtggt
      301 cgcaggggcc gcggaggcgc tcgggctggc ctcgctccaa cgcgcatatg cgccatcgac
      361 caccgacgtc gagcgcctcg ttgcgacgcc ctcgatctgg gtcgtgctgg ctcggagacc
      421 cgccgacctc gcggcgctcg cctcgcgtgg ctggacctcg atcaccccgg tacctccgct
      481 cacggacgac aacccggaca ttctccggct cctccgtctc tggcgttagg atgcgtgacg
      541 taataacttg acatgtagag gccggttcgg tatgctccgg gggtgatcga tgagggtgcc
      601 atccggacgc gctacggggc gttgtcggcg gccctcgacg aacggtcacg gcggctcttc
      661 ctcggggcgg aggcgctcgc cgccggacgg ggtggccagg ccgcggtgac ccgcgcgacg
      721 ggcgcctcgc ccgcgacgat ccggcgcggg ctcgtcgagc tcggtgcgcc cggtcagctc
      781 gcctcgggcc ggatccgccg gccgggtggc ggtcggaagc ggacggtcga gctcgacccg
      841 accctgcggg ccgacctgga agcgctcatc gagcccggga cccggggcga tcccgagtca
      901 cccctgcgct ggacgaccaa gagcgtccgg cggctcgccg ccgagctcgg acgcgggggc
      961 catcgggtga gctaccggat cgtcgctggg ctcctccacg agctcggcta cagcctccag
     1021 gccaaccgca aggtcctcga ggggacccac caccccgacc gcaacgccca gttcgagcac
     1081 ctcaacgcgg cggtgcagct gcagctgtcg ctgggtcagc ccgtcatcag cgtcgacacc
     1141 aagaagaagg agctggtcgg cccgttccgc aacgccggcc gggagcttcg tccgaagggc
     1201 gacccggagc gggtgctcat gcacgacttc gtcatcgacg agctcggccg ggcgagcccc
     1261 tacggggtct acgacgttgc ggcgaacgag gcctgggtga gcgtcggaac cgaccacgac
     1321 acggccgcct tcgccgtcga gagcatccgc cgctggtggg cgacgatggg ccaggagctc
     1381 taccccgacg cgaagcagct cctcgtcacc gccgatgccg gcggcagcaa cggctaccgc
     1441 ctccggctct ggaagctcga gctgcagaag ctcgccgacg agacgggcct cgagatcgcc
     1501 gtctgccact tcccgcccgg cacctccaag tggaacaaga tcgagcaccg gctgttcagc
     1561 gcgatcagcc agaactggcg gggcaagccg ctcgtcagcc acgaggtcat cgtcaagctc
     1621 atcgccgcga cgacgacccg gaccggcctt cgggtccgga gcgcgctcga tgagaaccac
     1681 tacccggccg ggcggaccgt ctccgacgcc gacatggaca ccctccatct ccggcccgac
     1741 gccttccacg gcgagtggaa ctactccctc ctgccccgtc tcatgctccc cgagggatga
     1801 ggagccttat ttcgtggcag tccctaagga cttgtcagct aattaccgat atgctatgct
     1861 ggcagggtga tcgatgaggc ggcgatcgga gcgcggttcc tagcggtcgc atccctgttc
     1921 gacgagcggg cccgccggct gctcgctggg tctgaggcgc tcgccatcgg acggggcggg
     1981 gtggcggccg tggcccgcgc gaccggcctc gcgcgcacga ccgtcgcgcg cggcgtggcc
     2041 gatgtccagg ccggggagac cgtcgccctt ggacgcatcc gccgggcggg cgctgggcgg
     2101 ccacccatcg agaagcgcga cccgaccctg cgcgcggacc tcgaggcgct catcgagccg
     2161 accagccggg gggacccgga gtcccccctc cgctggacga cgaggagcgt ccgcaacctc
     2221 gccgcggagc tgcgccgtca gggccacgcg gtcagccacc agaccatctc ggagctgctc
     2281 cacgacctgg gctacagtct ccaggccaac cgcaagacgc tcgaggggac gtcgcatccc
     2341 gaccgcgacg cccagttccg gcacatcaac gaggccgtcc agctgcagct cgccctgggc
     2401 gagccggtca tcagcgtgga caccaagaag aaggagctgg tgggtccctt ccgcaacggt
     2461 ggccgggagc tgcgcccgaa aggggacccc gagcgggtgc tgatgcacga cttcgtcatc
     2521 cccgagctcg gccgggcgag cccgtacggg gtctacgacg tcgccgccaa cgaggcctgg
     2581 gtgagcgtcg gcaccgacca cgacacggcg gccttcgcgg tcgagagcat ccgccgctgg
     2641 tggacgacga tgggcgcccc gctctacccc gaggccacgc gcctgctggt cacggccgac
     2701 gcgggcggga gcaacggcta ccggctccgc ctgtggaagc aggagctcgg caagctggcc
     2761 gacgagatcg gtctggagat cgccgtgtgc cacttcccgc cgggcaccag caagtggaac
     2821 aagatcgagc accgcctctt cagcgcgatc acccagaact ggcggggcaa gccgctcgtc
     2881 agccacgaga cggtggtcaa cctgatcgcc gcgacgacga cccggacggg cctgcgggtc
     2941 cggagcgagc tcgacaccag cgactacccg gccgggttac gggtgagcga cgcggacatg
     3001 gaaaccctct acctccgccg cgacgacttc cacggggagt ggaactactc gctcctgcca
     3061 cggcagcggc tatccctgac tgaggccgtt gtttcctgac ggtccctaag agcggcgaag
     3121 cagcatcgcg atgctcgtcg cggaggcgcg gatcccgagc tttgcgagct cgcctttgat
     3181 cctcaggtat ccccaccgag ggttctccct cgccatctga aggatcagct tgcagacctt
     3241 cggatcgatc gcagggcgcc cgagcgggcg atgcggcctc gtactttcgc gcgacgagtg
     3301 accggtgcca gcgcagaagc gtctcgggac gaacgaggaa cgcgccccag tgttccttgg
     3361 gaaggatcct gcttgccgca gcgagcagcg cgcggtcccg gcgccggtag accgggcgct
     3421 tgacctgccg gcgcaagacc tcgagctggt gacgaaggac gaggatctcg atc
//
LOCUS       DATAIX010000576         4408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_282843, whole genome shotgun sequence.
ACCESSION   DATAIX010000576 DATAIX010000000
VERSION     DATAIX010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282843"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VHM65_08500"
     CDS             complement(<1..688)
                     /locus_tag="VHM65_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX2705786.1"
                     /translation="MTLTPAPTVATTAHRPRLAINRLKERGPVDAATVDRFLARHPVP
                     VVEGDRVTFLFRGEADVVHIRHRVVGLADPLPLRRLAGTDLWFGTIELPALSRVEYQV
                     EIVRAGQVERVNDPLNPRVAHSPFGPSSVCAAEGYAVPDWAQPDPEARPGELIDLTVA
                     SRALRRDHPVRVYLPARFQGSVRYPLLVVHDGTDYLEYASAKPVLDNLIHRLDVAPLV
                     VAFIPPGDRLV"
     gene            complement(702..1622)
                     /locus_tag="VHM65_08505"
     CDS             complement(702..1622)
                     /locus_tag="VHM65_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705787.1"
                     /translation="MTTYLLGPQRFATTAGTVVRGLGQGGPVATVTSGWEEREGNDAE
                     LHAVLEGRSRNLHLYARLLDVIERDHHFARAAMAFRDAVDELGGFYSVRLHHAAQAVV
                     AVHRRTSRLGLGETALDESIAAVRAVDDWYSRELMRLYGELEATAPAGHSDVVMHHRH
                     EVASILQECTAVAVAGGHVGMLLRTLRLFAVAPAPDQPVVAWSAGAMAMTRRVVLFHD
                     FAPHGYGLPEVWDRGTGRVPGVVALPHARRRLRLDDPVRMMLFARRFADCTCVLLDDG
                     VKLAVGPDGELPPGTRYLAPDGSVALVGAR"
     gene            complement(1635..2858)
                     /locus_tag="VHM65_08510"
     CDS             complement(1635..2858)
                     /locus_tag="VHM65_08510"
                     /inference="COORDINATES: protein motif:HMM:NF014298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX2705788.1"
                     /translation="MNVIVVEPAFPQNQRLFVLALNRIGASVIGIGEMPLDHLDDELK
                     DAMRHYVHVGSVVDVGQLGAAVEYVQGMVWVDRLEATIEAHTLPTAQVREARHIPGTS
                     VRTTWLCRDKPSMKDALRAAGIPTAASAAADSAEEVIAFADRNGFPIVLKPRAGAGAQ
                     DTMRVDSWAGLQTALSTFADSGIGSIAVEEFVEGHEGFYDTISVGGAVRHDWATHYYP
                     NVLEAMRHRWISPQFITTNRIDQAPFYAEVRELGRQVIAALGIGTSATHMEWFFGPHG
                     LRFSEIGVRPPGVGCWDLYSAANDADVYREWALAVTGGQLTHELARTYSAGLVSLRPD
                     ADGQVRGYSGIEQIEDRYGEWVLDGHFPPQGSPTQAVEAGYMANAYVRMRHPDYDTLR
                     AMLDDAGRTIHVYAG"
     gene            2966..4264
                     /locus_tag="VHM65_08515"
     CDS             2966..4264
                     /locus_tag="VHM65_08515"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2705789.1"
                     /translation="MTLAERVRRLQVDTSPLRTSHDFRILFWAGTVFYLGSMVSYVAL
                     PFQLYQLTGSNLVVGAVGAVELVPLVVFGLWGGALADHGDRRSMLIWTGVAQAALTGV
                     LVANALLTNPRIWVIFVVAALLSAAQSLQRPSREALLPRTVSHDRLTAAVALSSLGLQ
                     TGMLAGPALGGLLLAHVGIGWCYAVDVTGLVVATGLFVRLGHYPVANRQSAPGLRSIS
                     DGIGYAARRRDLLGTYVVDLVAMFMAMPTVLYPAFAQTVLNTPSALGLLYSAGTVGSL
                     LATATSGWASRVHHHGRAVVLAAAAWGGAVALMGIAPSMWLAVFWGVVAGGADMVSAL
                     FRATLWNQTIPDSMRGRLAGIEMLSYSLGPLGGQVRAGLVADRTSVRASITSGGLLCV
                     LGVALSAAWLRGFWGYDARTDEHAVRERRLREQAQSGYDL"
BASE COUNT          586 a         1594 c         1587 g          641 t
ORIGIN      
        1 cgacgagccg gtcgcccggc gggatgaacg ccacgacgag cggtgccacg tcgaggcgat
       61 ggatgaggtt gtcgaggacg ggcttggccg aggcgtactc gaggtagtcg gtcccgtcgt
      121 gcacgacgag cagcgggtag cgcaccgacc cctggaagcg cgccggcagg tagaccctga
      181 ccgggtggtc ccggcgcagc gccctgctcg cgacggtgag gtcgatgagc tcccccggcc
      241 gcgcctccgg gtcgggctgc gcccagtcgg ggacggcata cccctcggcg gcacacaccg
      301 agctcggccc gaaggggctg tgcgcgacac gagggttgag cgggtcgttg acgcgctcga
      361 cctgcccggc ccgcacgatc tcgacctggt actcgacccg ggacagcgcc ggcagctcga
      421 tcgtcccgaa ccacaggtcg gtcccggcga ggcgccgcaa cggcagcggg tcggccagcc
      481 cgacgacgcg gtggcggatg tgcaccacgt cggcctcgcc acggaagagg aacgtcaccc
      541 ggtctccctc gacgaccggg acgggatgac gcgccaggaa ccggtcgacc gtcgccgcgt
      601 cgaccggccc tcgctccttg agccggttga tggccagccg gggccggtga gcggtcgtcg
      661 ccacggtggg cgcgggcgtc agtgtcatcg cgcacccctg gtcatcgcgc acccacgagt
      721 gcgactgacc catcgggggc gagatacctc gtgcccggtg gcagctcacc atcggggccg
      781 acggcgagct tgacgccgtc gtcgagcagg acgcaggtgc agtcggcgaa ccggcgcgcg
      841 aagagcatca tccggacggg gtcgtcgagg cgcagccggc ggcgggcgtg aggcagggcg
      901 acgacacccg ggacccggcc ggtccctcgg tcccagacct cggggaggcc atacccgtgg
      961 ggcgcgaagt cgtggaagag cacgacgcgg cgcgtcatcg ccatcgcccc ggcggaccac
     1021 gccaccaccg gctggtcggg ggccggtgcc acggcgaaga ggcgcagggt ccgcagcagc
     1081 atccccacgt gcccgccggc gactgccacg gcagtgcact cctgcaggat cgaggccacc
     1141 tcgtgccggt ggtgcatcac gacgtcgctg tgcccggccg gcgcggtggc ctcgagctcg
     1201 ccatacaggc gcatgagctc gcgggagtac cagtcgtcga cggcccgcac cgcggcgatc
     1261 gactcgtcga gggccgtctc cccgaggccg agccgcgacg tgcgccggtg gaccgccacc
     1321 accgcctggg cggcatggtg caggcgcacg gagtagaacc cgcccagctc gtcgacggcg
     1381 tcgcggaaag ccatggccgc ccgggcgaag tggtggtcac gctcgatcac gtcgagcagc
     1441 cgggcataca ggtgcaggtt gcgcgagcgc ccttcgagca ccgcgtgcag ctcggcgtcg
     1501 ttgccctcgc gctcctccca gcccgaggtg accgtcgcga ccggacctcc ctgcccgagc
     1561 ccgcgcacga ccgtcccggc cgtcgtcgcg aagcgctggg gcccgagcag gtaggtcgtc
     1621 atcgggcccg gccgtcaccc ggcgtagacg tggatcgtcc ggccggcgtc gtcgagcatc
     1681 gcgcgcagcg tgtcgtagtc cggatggcgc atccgcacgt aggcgttggc catgtagccc
     1741 gcctcgacgg cctgggtagg gctcccctgc ggggggaagt gcccgtcgag gacccactcg
     1801 ccataccggt cctcgatctg ctcgatcccg ctgtacccgc ggacctggcc gtcggcgtcg
     1861 gggcgcagcg agacgaggcc ggccgagtag gtccgggcca gctcgtgggt gagctggccg
     1921 ccggtcaccg cgagcgccca ctcacggtag acgtcggcat cgttggcggc cgagtagagg
     1981 tcccagcacc ccacgcccgg gggacgcacg ccgatctccg agaagcgcag gccatggggc
     2041 ccgaagaacc actccatgtg cgtcgccgag gtgccgatgc cgagcgcggc gatgacctgc
     2101 cgcccgagct cacgcacctc ggcatagaag ggagcctggt cgatccggtt cgtcgtgatg
     2161 aactgcggcg agatccaccg gtgtcgcatc gcctcgagga cgttggggta gtagtgggtc
     2221 gcccagtcgt gtcgcaccgc gcccccgacc gagatcgtgt cgtagaagcc ctcgtgccct
     2281 tcgacgaact cttcgacggc gatcgagccg atccccgagt cggcgaacgt gctcagcgcg
     2341 gtctgcaggc cggcccagga gtcgacgcgc atcgtgtcct gcgcaccggc acccgcgcga
     2401 ggcttgagca cgatcgggaa gccgttgcgg tcggcgaagg cgatgacctc ctcggccgag
     2461 tccgccgccg ccgatgcggc ggtcgggatc ccggcagccc gcagggcgtc cttcatcgag
     2521 ggcttgtcgc ggcacagcca ggtggtgcga accgaggtgc cggggatgtg gcgggcctcg
     2581 cgcacctgcg cggtcggcag cgtgtgcgcc tcgatcgtcg cttcgagccg gtccacccag
     2641 accattccct ggacgtactc gaccgctgcg cccagctgtc ccacgtcgac gacggacccg
     2701 acgtgcacgt agtgccgcat cgcgtccttg agctcgtcgt cgaggtggtc gagcggcatc
     2761 tcgccgatcc cgatgacgct cgccccgatc cggttcaggg cgaggacgaa cagccgttgg
     2821 ttctgtggga acgcgggctc gacgacgatg acgttcacaa cgtcgcacaa tatccgcatg
     2881 cgtccccgcg gacacctccg gcacactgtg cgggtgctgg tcccctgtgc tatgcggcca
     2941 cccccgccga ccgggagggc agggtatgac gctcgccgag cgggtgcgcc ggctccaggt
     3001 cgacacgtcc cccctgcgca cgtcccacga cttccgcatc ctgttctggg ccgggacggt
     3061 gttctacctc ggctccatgg tcagctacgt cgcgctgccg ttccagctct accagctcac
     3121 cgggtccaac ctcgtcgtcg gggcggtggg cgcggtcgag ctcgtgcctc tcgtcgtctt
     3181 cggtctgtgg ggcggggcgc tcgccgatca tggcgaccgt cgttcgatgc tcatctggac
     3241 cggcgtggcc caggcggccc tgacgggggt gctcgtggcc aacgcgctgc tgacgaaccc
     3301 gcggatctgg gtcatcttcg tcgtcgccgc gctgctgtcg gcggcccagt cgctccagcg
     3361 cccctcgcgc gaggcgctgc tgccccggac cgtcagccac gaccggctca cggccgcggt
     3421 ggccctgagc tcgctcggcc tgcagaccgg catgctcgcc gggccggcgc tcggcggact
     3481 cctgctcgcc cacgtcggga tcgggtggtg ctacgccgtc gacgtcaccg ggctcgtcgt
     3541 ggcgaccggg ctgttcgtgc gtctcgggca ctacccggtc gccaaccgcc agagcgctcc
     3601 gggcctgcgc tccatcagcg acgggatcgg gtatgccgcg cgccgccgcg acctgctcgg
     3661 cacctacgtc gtcgacctcg tcgcgatgtt catggccatg ccgacggtgc tctacccggc
     3721 gttcgcccag accgtgctga acacgccgag tgcgctcggg ctgctctact cggccggcac
     3781 cgtcgggtcg ctgctcgcga cggcaacctc gggctgggcc tcgcgggtcc accatcacgg
     3841 acgtgccgtg gtcctcgccg ctgcggcctg gggcggcgcg gtcgcgctca tgggaatcgc
     3901 tccctcgatg tggctcgcgg tgttctgggg cgtcgtggcc ggaggggccg acatggtcag
     3961 tgccctgttc cgcgcgacgc tctggaacca gacgatcccc gactccatgc gcggacgcct
     4021 cgccgggatc gagatgctgt cctactcgct cggcccgctc ggcggccagg tccgggccgg
     4081 gctcgtcgcc gaccggacca gcgtgcgcgc ctcgatcacc agcggtggcc tgctctgcgt
     4141 cctcggtgtc gccctcagcg cagcgtggct gcgcggcttc tgggggtatg acgcgcgcac
     4201 ggacgagcac gccgtccgcg aacgccgcct gcgggagcag gcacaatcgg ggtatgacct
     4261 ctgagcaacc cgtgacccgg tggggcgagc tgaccgggca cggcagcggt gctgcctacc
     4321 aggcccggtt cgacgcgctg gccaggtccg gcggtgaccc gcacggcgag gcgcacttcg
     4381 tcgcttcgtg ggtcgatgcg cccgcacg
//
LOCUS       DATAIX010000577         2310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_133718, whole genome shotgun sequence.
ACCESSION   DATAIX010000577 DATAIX010000000
VERSION     DATAIX010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133718"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VHM65_08520"
     CDS             complement(<1..1097)
                     /locus_tag="VHM65_08520"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2705790.1"
                     /translation="MSPSRRRAERGGLIGLGITLVAAAAGTAVGIAADRLNRDRRLAE
                     AMDTHDSLEITPDRELLVITEDGIPLHVEVDDPEQGVDPRKKTVVMSHGYCLSSRCWV
                     FQRRALKAAGYRVVVWDQRGHGQSGKADPSSYTIDQVGRDLAAVIHATAPTGPLLLVG
                     HSMGGMTIMAMAQTHPQIIRERVVGLALVATSSGGIPLARGGPAAFGKMMLGRFGPGL
                     FGELAKRQTTINTVLAVNEDLEDFVVNRLSFGSPVPRSIVRHTREMLLGTDLTVVAGF
                     APTFDTHDKTPAMQTFLGTETLVFNGDKDSLTPPEHSESIVRLIPGAEHIVIKDAGHI
                     IMLEHPALLNEQLLVEQRGVLEHDDVPGVLD"
     gene            complement(1094..2287)
                     /gene="alr"
                     /locus_tag="VHM65_08525"
     CDS             complement(1094..2287)
                     /gene="alr"
                     /locus_tag="VHM65_08525"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX2705791.1"
                     /translation="MSALPSCARVDLDAITANVRILREHAPTASVMAVVKADGYGHGL
                     LPSARAALSGGASWLGVAQLGEALALRAAGITAPTLTWLHVPGAAFTEAVEAGIDLGV
                     SADWAVAEIVAAARETGRTARIHLKVDTGLSRGGVMPADLPAALDGIGRAVAEESVEL
                     VGVWSHFAWADDPDHPTVRRQHEVFVETVRTIEAAGLRPQIRHLANSAATLTNPAAHF
                     DLVRPGLAVYGLSPVPQLGGPEHFGLVPAMTLIARLSSVKRVPRGSGVSYGHQYVTER
                     DTVLGLVPMGYADGIPRNATNVGPLAVAGARHTIAGRVCMDQVVVDLGPDSAAAAGDE
                     VLLFGPAGPAGSAGSAGPSAQDWADATGTISYEIVTRIGARVPRRYAGALASETAAAG
                     KGEAQ"
BASE COUNT          344 a          799 c          824 g          343 t
ORIGIN      
        1 tgatcaagga cgccgggcac atcatcatgc tcgagcaccc cgcgctgctc aacgagcagc
       61 tgctcgttga gcagcgcggg gtgctcgagc atgatgatgt gcccggcgtc cttgatcacg
      121 atgtgctcgg cgcccgggat gagccgaacg atcgactcgc tgtgctctgg cggagtgagg
      181 ctgtccttgt ccccgttgaa cacgagtgtc tccgtgccga ggaaggtctg cattgcgggg
      241 gtcttgtcat gcgtgtcgaa ggtcggtgcg aagccggcca ccacggtcag gtcggtcccc
      301 aggagcatct cgcgggtgtg ccgcacgatg ctgcgcggca cgggtgaccc gaaggagagg
      361 cggttgacga cgaagtcctc gaggtcctcg ttgacggcga gcactgtgtt gatcgtcgtc
      421 tgacgtttgg ccagctcgcc gaacaacccc ggcccgaacc gacccagcat catcttgccg
      481 aatgccgcgg ggccacccct cgcgagcggg atcccgccgg agctggtggc gaccaaggcc
      541 agaccgacga cccgctcacg gatgatctgc gggtgggtct gggccatggc catgatcgtc
      601 ataccgccca tcgagtgacc gacgagcagg agcggtccgg tgggtgcggt cgcgtggatc
      661 accgcggcca ggtctcgccc gacctggtcg attgtgtacg acgaggggtc ggccttgccg
      721 gactgcccgt ggccccgctg gtcccacacg acgacgcggt agccggcggc cttgagcgcc
      781 cggcgctgga acacccagca gcgcgagctc aggcagtagc cgtgcgacat gacgacggtc
      841 ttcttcctcg ggtcgacacc ctgctccggg tcgtcgacct cgacgtggag cggaatgccg
      901 tcctcggtga tgacgaggag ctcgcggtcg ggggtgatct ccagcgagtc gtgggtgtcc
      961 atcgcctcgg ccagccgacg gtcccggttg agccggtcgg cggcgatccc gactgcggtg
     1021 ccggccgcag ccgcgacaag ggtgatgccg aggccgatca ggccaccacg ctcagccctc
     1081 ctcctcgatg ggctcattgg gcctcgcctt tccccgccgc ggcggtctcg ctcgccaacg
     1141 ccccggcata cctccggggc acccgtgcac cgatgcgcgt cacgatctca tagctgatcg
     1201 ttcccgtcgc gtccgcccag tcctgggcgc tgggcccggc cgacccagcc gacccagccg
     1261 gcccggccgg cccgaacaac aacacctcat cccctgccgc cgctgcgctg tccgggccga
     1321 ggtcgacgac cacctggtcc atgcataccc ttccggcgat ggtatgacga gcccccgcca
     1381 cggcgagagg gccgacgttg gtggcattgc gcgggatccc gtcggcatac cccatgggca
     1441 cgagcccgag aaccgtgtcg cgctcggtga cgtactggtg gccgtagctc acgcccgaac
     1501 cgcgaggaac ccgcttgacc gaggagagcc gcgctatgag agtcatggcc gggacgagac
     1561 cgaagtgctc gggcccgccg agctggggca ccggcgagag gccgtagacg gccaggccgg
     1621 gccggacgag gtcgaagtgg gcagccgggt tcgtcagcgt ggcagcggag ttggccagat
     1681 ggcggatctg cggacgcagc ccggctgcct cgatggtgcg caccgtctcc acgaacacct
     1741 cgtgctggcg gcggaccgtc gggtggtcgg ggtcgtcggc ccaggcgaag tgcgaccaca
     1801 ccccgacgag ctcgacggac tcctcggcca cggcgcgccc gatgccgtcg agagcggcgg
     1861 gcaggtcggc cggcatgacc ccgccccggg acagccccgt gtcgaccttg aggtggatcc
     1921 gggccgtccg gccggtctcc cgggctgcgg cgacgatctc cgccaccgcc cagtccgccg
     1981 agacgcccag gtcgatcccg gcctcgacgg cttcggtgaa tgcggcacct gggacatgga
     2041 gccaggtcaa ggtcggcgcc gtgatgccgg cggcccgcag cgcgagggct tcgcccagct
     2101 gggcgacgcc cagccaggac gcgccgcccg agagggccgc ccgggcgcta gggagcaggc
     2161 catgtccgta cccgtcggcc ttgacgacgg ccataacgct cgcggtcggt gcgtgctcgc
     2221 gcaggatacg cacgttcgcg gtgatggcgt cgaggtcgac tcgggcgcac gaggggaggg
     2281 cggacatagc gggatcagtc tgtcagatcc
//
LOCUS       DATAIX010000578         2632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_282934, whole genome shotgun sequence.
ACCESSION   DATAIX010000578 DATAIX010000000
VERSION     DATAIX010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282934"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VHM65_08530"
     CDS             complement(<1..315)
                     /locus_tag="VHM65_08530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2705792.1"
                     /translation="MTLAIRPLDVHDDADCARAYEIFRAATAFERPHASTPSLAERVA
                     EWRHEDPAERMEMAVAVQGTEIVGVGEAYFPLADNTFMVWANVGVEPHRRRQGIGGAL
                     MAH"
     gene            complement(358..>2632)
                     /gene="treY"
                     /locus_tag="VHM65_08535"
     CDS             complement(358..>2632)
                     /gene="treY"
                     /locus_tag="VHM65_08535"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX2705793.1"
                     /translation="LNDEAGGREGFDAMAARLHELGLGLVVDVVPNHMAVPTPAYLNR
                     PLWSVLREGPASPYARWFDIQWAAERHALLMPMLGARIGQVLLAGELTIDAAGGPGGD
                     ETVLRYHEHEFPVRPGTAHLPLAELVERQWYRLAHWRVADDELNYRRFFDIDTLAAIR
                     VEDPEVFAPTHALLLELVRGGAIDGLRIDHPDGLADPRGYLRHLRSAAPDAWVVVEKI
                     LEGDESLPADWPCAGTTGYDALLRLGGVFVDPAGAGPFSALLTELTGDRAGFVEVAHA
                     AKAEVTSGLLYTEVERLTAVLADICAADVLLRDHTRRALQGAVTALLVAIGRYRAYVV
                     VGEEPGPETVAVIDAAVARAAISVPEPDPDALALVRDLVLGRPVGSPHRVGDAARAEV
                     VIRFQQACGPVMAKGVEDTAFYRWPRLTALNEVGGDPGRFGVSPDELHAYAARIAHDW
                     PATMTALTTHDTKRSEDVRARLAVLSEVPGEWVTWLREVRETAIPHRDPALDPRTEYL
                     IWQTLVGTWPIGAERLEAHAVKAVREAKDHTSWTTPVASYEAAVTAFVRGVLADPSCT
                     AALDGWVARHTGAERCVVLGQKLLQLVLPGVPDTYQGTELVMASLVDPDNRGEADLDR
                     PRTRLAALDAGSAPADLDDEKLLVTSRALRLRREHAGWFTGPAARTGPVAATSAHALA
                     LGRGTGGVLSVVAVVTRLAVGLDRLGGWAEHTLALPPGLWRDVLTGRQVRTAGAGARL
                     ADLLADLPVGLLVRVGR"
BASE COUNT          387 a          974 c          950 g          321 t
ORIGIN      
        1 atgagccatc agggccccac cgatcccctg gcggcggcgg tggggctcga caccgacgtt
       61 cgcccagacc atgaaggtgt tgtccgccag ggggaagtaa gcctcgccca caccgacgat
      121 ctccgtaccc tgcaccgcca cggccatctc catccgctcc gccgggtcct cgtggcgcca
      181 ctcggcgacc cgctcggcca ggctcggtgt cgacgcatgt ggacgctcga acgccgttgc
      241 cgcccggaag atctcgtagg cgcgcgcgca gtcggcgtcg tcgtggacgt cgaggggcct
      301 gatggcaagg gtcacgacat cgagggtaac cagcgatgcc gcgcggggac gacaggttta
      361 tcggccgacc cgcacgagca gccccaccgg caggtccgcg agcaggtccg ccagccgggc
      421 accggcgccg gccgtgcgga cctggcgccc cgtcagcaca tcgcgccaca gccccggcgg
      481 cagggccagg gtgtgctcgg cccagccgcc gagccggtcc agccccaccg cgagccgggt
      541 gacgacggcc acgaccgaga gcacgccgcc cgtgccgcgc ccgagcgcca gcgcatgagc
      601 cgatgtcgct gcgaccggcc cggtgcgggc ggccggaccg gtgaaccacc cggcgtgctc
      661 gcggcgcagc cgcagcgccc gggaggtgac gagcagcttc tcgtcgtcga gatcggccgg
      721 cgccgacccc gcatcgaggg cggccagccg ggtgcgcggc cggtcgaggt cggcctcgcc
      781 acggttgtcg gggtcgacga gggacgccat gaccagctcg gtgccctggt aggtgtccgg
      841 cactcccggc agcacgagct ggagcagctt ctggccgagc acgacacacc gctcggctcc
      901 cgtatggcgc gccacccacc cgtcgagcgc agccgtgcag gaggggtcag cgagcacacc
      961 ccggacgaac gcggtcacgg cggcttcgta ggaggccacc ggggtcgtcc agctcgtgtg
     1021 gtccttggcc tcgcggaccg ccttgacggc atgcgcctcg aggcgttcgg cgccgatcgg
     1081 ccaggtcccg acgagggtct gccagatcag gtactccgtc ctcgggtcga gggcagggtc
     1141 ccgatgcggt atggcggtct cccgtacctc gcgcagccag gtcacccact cgccgggaac
     1201 ctcgctcagc acggccagcc gagcccggac gtcctcgctg cgtttggtgt catgcgtcgt
     1261 cagggcggtc atcgtcgccg gccagtcatg ggcgatccgg gcggcatacg cgtggagctc
     1321 gtcggggctc acaccgaagc ggcctgggtc gcctccgacc tcgttgaggg cggtgagccg
     1381 cggccagcgg tagaacgccg tgtcctcgac acccttggcc atgaccggcc cgcaggcctg
     1441 ctggaaccgg atgacgacct cggcccgcgc ggcgtcgccc acgcgatggg gcgaacccac
     1501 cggacggccg aggacaaggt ctcggacgag cgccagggca tcggggtccg gctccggcac
     1561 cgagatcgcc gcccgagcca ccgccgcgtc gatgaccgcg accgtctcgg ggccgggctc
     1621 ctcgccgacc acgacatagg cccggtaccg gccgatggcg acgagcagcg ccgtgaccgc
     1681 cccctgcagc gcgcggcggg tgtggtcgcg cagcaggacg tcggccgcgc agatgtcggc
     1741 cagcacggcg gtcagtcgct cgacctcggt gtagagcagc cccgaggtga cctcggcctt
     1801 ggccgcgtgc gcgacctcca cgaaccccgc ccggtcaccc gtgagctcag tcagcaacgc
     1861 cgaaaacggc ccagcaccag cgggatcgac gaagacccca ccgaggcgaa ggagcgcgtc
     1921 atacccggtg gtcccggcgc acggccagtc agcaggcaag gactcgtcgc cctcgaggat
     1981 cttctcgacg acgacccacg catccggggc ggccgagcga aggtgccgga ggtagccgcg
     2041 cgggtcggcc agcccgtcgg ggtggtcgat gcgcagcccg tcgatggcac cgccacggac
     2101 aagctcgagc agcagcgcgt gcgtgggcgc gaacacctcg gggtcctcga cccggatcgc
     2161 ggcgagggtg tcgatatcga agaaccggcg gtagttgagc tcgtcgtcgg cgacccgcca
     2221 gtgcgcgagg cggtaccact ggcgctcgac gagctcggcg agcggcaggt gcgccgtccc
     2281 tggacgcacc gggaactcgt gctcgtggta gcgcagcacg gtctcgtcgc cgcccgggcc
     2341 gcccgccgcg tcgatggtca gctcacccgc gagcaggacc tgcccgatcc gcgccccgag
     2401 catcggcatc agcagcgcgt ggcgctcggc ggcccactgg atgtcgaacc agcgagcgta
     2461 gggcgaggcc ggcccctccc gcagcaccga ccagagcggc cggttgaggt aggccggcgt
     2521 cggcacggcc atgtggttgg gcacgacatc gacgacgagg cccaggccga gctcgtgcag
     2581 ccgcgcggcc atcgcgtcga acccctcgcg ccctccggcc tcgtcgttga gg
//
LOCUS       DATAIX010000579         2501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_194026, whole genome shotgun sequence.
ACCESSION   DATAIX010000579 DATAIX010000000
VERSION     DATAIX010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194026"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..410)
                     /gene="clpS"
                     /locus_tag="VHM65_08540"
     CDS             complement(120..410)
                     /gene="clpS"
                     /locus_tag="VHM65_08540"
                     /inference="COORDINATES: protein motif:HMM:NF000668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX2705794.1"
                     /translation="MSLAPTVEPTTSDSVALAPDPGWVTLVWNDPVNLMSYVSWVFQS
                     YFGYPRRKADKLMLDVHQLGKAAVSHGTLEEMERDTEAMQGYGLWATFEKDG"
     gene            448..1767
                     /locus_tag="VHM65_08545"
     CDS             448..1767
                     /locus_tag="VHM65_08545"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF006698.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX2705795.1"
                     /translation="MTSLPSLPPSAPSTALLTDHYELTMLQAALRSGAAHRRCVFEAF
                     ARRLPEGRRYGVVAGTGRLVEALAGFRFGEAELEALVRMKVVDGATIDYLAGYRFTGD
                     IRGYAEGECFFSGSPVVVVESSFAEGVLLETLILSILNHDSATASAASRMTTAAGERP
                     CIEMGSRRTHEQAAVASARAAYIAGFAATSNLEAGRRYGVPTAGTAAHAFTLVHDDEE
                     EAFGAQLDALGTGTTLLVDTYDVTAAVQTAVRLAGPALGAVRLDSGDLLVQAWEVRAQ
                     LDSLGAAGTRIIVTSDLDEYAIAALAVAPVDAYGVGTCLVTGSGAPTAGMVYKLVVRA
                     DDEGVMQPVAKKTKDKLSVGGRKWAMRRLGGAGRAEAEVIGIGEPPRGDSNDRPLMTD
                     LVRAGEVVAEPDIAAARARHTASRAELPAVARQLQKGDPCIPTLYER"
     gene            1776..2396
                     /locus_tag="VHM65_08550"
     CDS             1776..2396
                     /locus_tag="VHM65_08550"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX2705796.1"
                     /translation="MLPSLARASGDSPLWSDLMTFGVPPAEKVTRTVLVYLAIWLLLR
                     LAGKRQLAQLSSFDLVVVLLLSNVVQNAIIGQDNSLVGGLLGAAVLVLLNSAVGRLAL
                     TYPRAARWFEGSPTVIASDGQADEAGLRRVGLSQAELLHAIRLQGADELEQTQDVELL
                     PNGMVMMELKPEERTVSRRELDDAIAALRGHLDARLDRIEASLARS"
BASE COUNT          349 a          895 c          887 g          370 t
ORIGIN      
        1 gctcgggcgg tgcgagcagc gcccgcgtct gctccatcaa cccggtgagg atcccgcgct
       61 ccacgacgtc gaggcgagcg acataccggc ccttcttgtg cgcgaaggcc cgcgccatct
      121 cagccgtcct tctcgaaggt cgcccacagc ccgtagccct gcatggcttc ggtgtcacgc
      181 tccatctcct cgagcgtgcc gtgcgacacg gccgccttgc ccaactggtg gacgtcgagc
      241 atgagcttgt cggccttgcg gcgcggatag ccgaagtagg actggaagac ccacgacacg
      301 taggacatga ggttgaccgg gtcgttccac acgagggtga cccaccctgg gtccggggcg
      361 agggcgacgg agtcgctcgt cgtcggctcg acggtggggg cgagggacac acggccactg
      421 tattcgctcc ctcgtacgct gagccttgtg acgagcctgc cgagtctgcc gccgagcgcg
      481 cccagcaccg ccctcctgac cgaccactac gagctgacga tgctgcaggc agcccttcgc
      541 agcggcgcgg cgcaccgccg ctgtgtcttc gaggccttcg cgcggcgcct gcccgaagga
      601 cggcggtacg gcgtcgtggc cggcaccggg cgcctcgtcg aggccctggc cggcttccgc
      661 ttcggtgagg ccgagctcga ggcgctggtg cggatgaagg tcgtcgacgg cgcgaccatc
      721 gactacctcg cgggctaccg cttcaccggt gacatccgcg ggtatgccga gggcgagtgt
      781 ttcttctctg ggtcaccggt cgtcgtcgtc gagtcgagct tcgccgaggg ggtgctcctc
      841 gagacgctga tcctgtcgat cctcaaccac gacagcgcga cggcgtcagc ggcgtcccgc
      901 atgacgaccg ccgcgggcga gcggccctgt atcgagatgg gctcgcggcg gacccatgag
      961 caggccgcgg tcgcgagcgc gcgggcggcc tacatcgcgg ggttcgccgc gacgagcaac
     1021 ctcgaggccg ggcgccggta tggcgtccct accgccggca ccgcggcgca cgccttcacc
     1081 ctggtccacg acgacgagga ggaggcgttc ggcgctcagc tcgatgcgct cgggaccggc
     1141 acgaccctgc tcgtcgacac gtatgacgtc accgcggcgg tccagacagc cgtccgcctg
     1201 gccgggcccg cgctcggcgc cgtgcggctc gactcgggcg acctgctcgt ccaggcgtgg
     1261 gaggtccggg cccagctcga ctcgctcggt gccgccggga cccggatcat cgtcacctcc
     1321 gacctcgacg agtacgccat tgccgcgctc gccgtcgccc cggtcgacgc ctatggcgtc
     1381 ggcacctgcc tcgtcaccgg gtcgggtgcc ccgacggcgg ggatggtcta caagctcgtc
     1441 gttcgggccg acgacgaggg ggtgatgcag cccgtcgcga agaagaccaa ggacaagctc
     1501 tcggtcggcg ggcgcaagtg ggcgatgcgc cgcctgggag gggccgggcg cgccgaggcc
     1561 gaggtcatcg gcatcggcga acccccgcgg ggcgacagca acgaccgccc gctcatgacc
     1621 gacctcgtcc gggccggcga ggtcgtggcc gagcccgaca tcgcggcggc ccgggcgcgt
     1681 cataccgcgt cacgagccga gcttcccgcg gtggcgcgcc agctgcagaa gggcgacccc
     1741 tgcatcccga cgctctacga aaggtaggag gcaccgtgct cccctcactc gcccgcgcat
     1801 cgggcgactc gccgctctgg tcggacctga tgaccttcgg ggtgccgccg gccgagaagg
     1861 tgacgcgcac cgtgctcgtc tacctggcga tctggctcct cctccgcctc gccggcaaac
     1921 gtcagctggc gcagctgagc tcgttcgacc tggtcgtcgt cctgctgttg tccaacgtgg
     1981 tccagaacgc catcatcggc caggacaact cgctcgtcgg ggggctgctc ggagcggcgg
     2041 tgctcgtcct gctcaactcc gccgtgggcc ggctggcgct gacctacccg agggctgcgc
     2101 gatggttcga gggctcaccg acggtcatcg ccagcgacgg ccaggccgac gaggcggggc
     2161 tgcgccgggt cggcctgtcc caggccgagc tgctccacgc catccggctc cagggggccg
     2221 acgagctgga gcagacccag gacgtcgagc tgctgcccaa cgggatggtg atgatggagc
     2281 tcaagcccga ggagcgcacg gtcagccgca gggagctcga cgacgcgatt gcggcacttc
     2341 gggggcatct cgacgctcgc cttgaccgca tcgaggcgag cctcgcccgg tcgtgaccgg
     2401 ggccgacatg ccgtcggcgg ctcagaccat cttgacgacg atgtcgtgca ggacctcgcc
     2461 ggcccgggcg acccggtcac cggcgttgga cagatggcgg t
//
LOCUS       DATAIX010000580         3270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_283044, whole genome shotgun sequence.
ACCESSION   DATAIX010000580 DATAIX010000000
VERSION     DATAIX010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283044"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            382..531
                     /locus_tag="VHM65_08555"
     CDS             382..531
                     /locus_tag="VHM65_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705797.1"
                     /translation="MGPNTTFMDLVLLAGAIGLGGITILVLLIASSARARTHPRIPHR
                     LHLRH"
     gene            677..1306
                     /locus_tag="VHM65_08560"
     CDS             677..1306
                     /locus_tag="VHM65_08560"
                     /inference="COORDINATES: protein motif:HMM:NF006743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HEX2705798.1"
                     /translation="MELDALARRARSLVPGTGRAILGICGAPGSGKSTLAARLVAEVN
                     RQEGTGTATQVPMDGFHLADVALDRLGLRDLKGAPETFDALGYAALLRRLREESDTTV
                     YAPGFERDLEQPIAGAIAVAPDARLVVTEGNYLLLADNPWPRVRSALDECWYVETEET
                     TRVQRLVARHIAFGKSPEAARVWVRHVDEPNAALVRASRGRADLVVALD"
     gene            complement(1453..2856)
                     /locus_tag="VHM65_08565"
     CDS             complement(1453..2856)
                     /locus_tag="VHM65_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemopexin repeat-containing protein"
                     /protein_id="HEX2705799.1"
                     /translation="MTRLVGLVAPSGQRAYFFSGDQYVRYDVAADAVDAGYPKPIAGS
                     WPGLFTRDIDAAVVLPNGKVCFFSGDQYIRYDWDADQADPGYPKAIAGSWPGLFVPVE
                     AAVTWDTGKVYFFQGQHYARYDPATGQIEDGYPKVIADGWPGVFVEAIESALWWPSGN
                     VYFFSADQYVKYDPALDAVPPGYPRPIAGNWPGLPIGAAQPSGGGGTTTPGALDAIPA
                     RALSAADALGELTRWQNEGLITFATSGMAGKVDLDGRDPATGAAVAGSVAGVIVRYWG
                     SGTKTGGPPSGGNAPDRLDPRHAVALVRFCRWLHDTWGVTELYHAGVNGDGSGARVDC
                     HGQGRALDFVGVLGTVNATEFTLTVFDDWGRVTTPSTPGGSWTPAGTGTTHFRLDDAP
                     DRTFERDVFRAIYDFAAGQWQDRSSGPDPAGAPTAIGAASFLMNPDHHTSSPGQPNGR
                     EAHQGHLHMQIGVTGTA"
     gene            3057..3215
                     /locus_tag="VHM65_08570"
                     /pseudo
     CDS             3057..3215
                     /locus_tag="VHM65_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          465 a         1147 c         1189 g          469 t
ORIGIN      
        1 cccaccaggg ccccgacggc ggcggcccgg tcgcggccat cgtcgacctc ggcgcgggcg
       61 agcccaccgg ttaccgctgg actcgctgac cgtcatcgcc ggcttcatcc tccaggccgg
      121 ctgccctcct gaccggcact cgcgcgtatg gcgccgccgc tcacctcacc gggtaggcgg
      181 cgacgctcgc gtcttgggga gacgcacggc ggcccgagac cgatgcaggt ccgggcagtc
      241 gggtcacagt tcggtctcga tggcaaccaa acgggcaccg gcgcggtcat agtcagtgag
      301 tctcacttgg ggacttgcgt tgggccccca cggcgactgg tccgcggcac cagcccaccg
      361 cacgaggaca ggggtgatct cgtgggacca aacaccacct tcatggatct cgtcctgctg
      421 gccggcgcta tcggcctcgg gggcatcacg atcctcgtcc tgctcatcgc cagctccgcg
      481 cgggcccgga cgcacccacg catcccccac cggctgcacc tacgtcacta gagggctacc
      541 acggcgaggg cgcgagatgc ctcaatgggt tgccaagacc cggtcgaagc caccagttga
      601 ggcatctcgc gcctcgggag gcccgggcct gcaggtgcga ggcccgttct gcacgtacgt
      661 cactagagtt cagcgggtgg agctcgatgc cctcgcccgc cgggcccgat cgctcgtgcc
      721 cggcaccggc cgggcgatcc tcggaatctg cggggccccg ggctcgggca agtcgaccct
      781 cgccgcgcgg ctcgtcgccg aggtgaaccg gcaggagggc accggcacgg ccacgcaggt
      841 ccccatggac ggcttccacc tcgccgacgt cgcgctcgac cggctcggac tgcgtgacct
      901 caagggggcg ccagagacct tcgacgccct cgggtacgcc gcgctgctgc gccgtctgcg
      961 cgaggaaagc gacacgacgg tctatgcgcc cgggttcgag cgcgacctgg agcagccgat
     1021 tgccggggcc atcgcagtcg cgcccgacgc ccgcctcgtc gtcaccgagg ggaactacct
     1081 gctccttgcc gacaacccct ggccgcgggt caggtcggct ctcgacgagt gctggtacgt
     1141 cgagaccgag gagacgaccc gggtgcagcg gctcgtggcg cgtcatatcg cgttcgggaa
     1201 gtcccccgag gccgcacggg tgtgggtcag gcacgtcgac gagcccaacg cggccctggt
     1261 ccgcgcctcg cgcggccgcg ccgacctcgt cgtcgcactg gactgacccg tcgggtcctg
     1321 aggcggccgt cggcgatcgg gcgggggtga gcagaagaag gcacgggcgc ctcccggcgc
     1381 gacgaagcca gtcgttctcg gcgtcgagcc tccgggtcac tcacgtcggg tgccggtcac
     1441 acggtgcgcc ggtcacgcgg tccccgtcac tccgatctgc atgtgcagat gcccctggtg
     1501 ggcctcccgg ccgttgggct gtccgggcga ggaggtgtgg tggtccgggt tcatgaggaa
     1561 gctcgccgcg ccgatcgccg tgggtgcccc cgcggggtcg ggtccggacg agcggtcctg
     1621 ccactgaccg gccgcgaagt cgtagatcgc ccggaagacg tcccgctcga aggtccggtc
     1681 cggggcgtcg tcgaggcgga agtgagtggt gcccgtcccg gccggggtcc agctgccgcc
     1741 cggcgtcgac ggggtcgtca cgcggcccca gtcgtcgaac accgtcagcg tgaactcggt
     1801 cgcgttgacc gtgcccaaga cgccgacgaa gtcgagggcg cggccctgac cgtggcagtc
     1861 gacccgggcg ccgctgccgt cgccgttgac cccggcatgg tagagctcgg tgacccccca
     1921 cgtgtcgtgc agccagcggc agaaccggac gagcgcgacg gcatggcgcg ggtcgagccg
     1981 gtccggggcg ttgccgccgg agggcgggcc gccggtcttc gtccccgaac cccagtagcg
     2041 cacgatcacc ccggcgacgc tgcctgccac agccgcgccc gtcgcggggt cgcgcccgtc
     2101 gaggtcgacc ttgccggcca tgccggatgt cgcgaacgtg atgagtccct cgttctgcca
     2161 ccgggtcagc tcaccgagcg cgtcggcagc gctgagtgca cgagccggta tggcgtccag
     2221 cgcacccggg gtggtggtcc cgccaccgcc ggagggctgg gccgccccga tgggcaggcc
     2281 tggccagttc cccgcgatcg gacgcgggta gcccgggggc acggcgtcca gagccgggtc
     2341 gtacttgacg tactggtcgg cgctgaagaa gtagacgttg ccgctcggcc accacagggc
     2401 cgactcgatc gcctcgacga acacgccggg ccagccgtcc gcgatgacct tcgggtagcc
     2461 gtcctcgatc tgcccggtgg ccgggtcgta gcgggcgtag tgctggccct ggaagaagta
     2521 gaccttgccc gtgtcccagg tgacggccgc ctcgacgggg acgaacaggc ccggccagct
     2581 gcccgcgatc gccttgggat agcccggatc ggcctggtcg gcgtcccagt cgtagcggat
     2641 gtactggtca ccggagaaga agcagacctt gccgttgggc aggacgacgg cggcgtcgat
     2701 atcccgggtg aacagacccg gccagctgcc ggcgatcggc ttcgggtatc ccgcgtccac
     2761 cgcatcggca gcgacgtcat accggacgta ctggtcaccg ctgaagaagt aggcccgctg
     2821 cccgctggga gcgaccaggc cgacgagtcg ggtcattgga catgcctcct ggcacttcaa
     2881 ccgtagatcg tgaacatgct gtcggcgtgg cggtgccggg tgtgaggcgg ccaccgcaca
     2941 atactccgaa gctttgtgga ctgaagaggc tcctgcggtg gccgcgtgcc acagcgtaga
     3001 cccgcaccgg tggaaccagg gaccggagca cctgcgggac gcctccggtc ggctgctcga
     3061 tcgccgagga cgcaggcgag gtatctgcgg atgggatgca gcatctgctg tcgcgggcga
     3121 gctgggacaa cgtcggggtc cgggacgacc tgcgcggcta cgtgctcgag cacctgggcg
     3181 acccggacgc ggtcctggtc gtcgatgaga cgggcacgtg aggaagggcc ggtgcgccgc
     3241 ggcaggggtg ccggaggaga ccgggttggc
//
LOCUS       DATAIX010000581         1773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_252994, whole genome shotgun sequence.
ACCESSION   DATAIX010000581 DATAIX010000000
VERSION     DATAIX010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252994"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VHM65_08575"
     CDS             complement(<1..526)
                     /locus_tag="VHM65_08575"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX2705800.1"
                     /translation="MTYPPLGAAGEPLGPAAVARYSRHLLLPEVGFEGQARLAASRVA
                     VVGAGGLGSATVTYLAAAGVGTLGIIDDDRVEASNLQRQVIHGESDVGRLKTESAEES
                     VARINPGVDVIRHDVRLEAANALEILGGYDLVVDGADNFPTRYLVNDACVRLGIPDVW
                     GSIFRFDGQVSVWWA"
     gene            610..1251
                     /locus_tag="VHM65_08580"
     CDS             610..1251
                     /locus_tag="VHM65_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2705801.1"
                     /translation="MALGTDSRVRGQRLPRSARRAQLLEAAQAAFVEFGYHAAAMDDI
                     ADRAGVSKPVLYQHFPSKLELYLALLDQQSSELLQLVRDALAATGDNKARVMATVAAY
                     YEFVSRDGAPFRLIFESDLTSEPAVSERLEQVSLSCADALATVIAADTGVSREDARVI
                     GMAQISARDWLSQGSPIPREEAAGLVGQLAWRGLGGFPKVTRPDREGPEPAGR"
     gene            1296..1520
                     /locus_tag="VHM65_08585"
     CDS             1296..1520
                     /locus_tag="VHM65_08585"
                     /inference="COORDINATES: protein motif:HMM:NF022749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HEX2705802.1"
                     /translation="MEVRIGVQNVAREITFESEQTADEVRAVVTAALAGGEVLTLVDD
                     KGRLVIVPTRALGYVHLGESEKSRFGFGTS"
BASE COUNT          285 a          650 c          595 g          243 t
ORIGIN      
        1 cggcccacca caccgagacc tgcccgtcga agcggaagat cgacccccac acgtcgggga
       61 tcccgaggcg cacgcaggcg tcgttgacga ggtagcgggt cgggaagttg tcggccccgt
      121 cgacgacgag gtcatacccg ccgaggatct ccagcgcgtt ggccgcctcg agccgcacat
      181 cgtggcgaat gacgtcgacc ccagggttga tccgggccac ggactcctcc gccgactccg
      241 tcttgagccg gccgacatcg ctctcgccgt ggatgacctg ccgctgcagg ttggacgcct
      301 cgacccggtc gtcgtcgatg atcccgaggg tcccgacccc cgccgccgcg aggtaggtga
      361 ccgtcgccga cccgaggccg ccggccccga cgacagccac ccgcgacgcc gcgagccgcg
      421 cctggccttc gaacccgacc tcgggcagga gcaggtggcg cgagtagcgg gcgactgccg
      481 cgggaccgag cggctcgccc gcagcgccga gcggggggta ggtcataagg tcgagggtac
      541 gaccctctag gatcagcggt cagggctcac cacgaagacc tggctcacca cgaagaccta
      601 gggacgggta tggcgctcgg gacggactcg cgggtacgcg gccagcggct gccccgctcg
      661 gcccgccgcg cgcagctgct cgaagcagct caggcagcct ttgtcgagtt cggctaccac
      721 gcagccgcca tggacgacat cgcagaccgt gcgggagtct ccaaacccgt tctctaccag
      781 cacttcccga gcaagctcga gctctacctc gcgttgctcg accagcagag cagcgagctc
      841 ctgcagctcg tccgcgacgc cctggccgcg accggcgaca acaaggcccg cgtcatggcg
      901 accgtcgcgg cctactacga gttcgtcagc cgcgacgggg caccgttccg gctcatcttc
      961 gagtccgacc tgaccagcga gcccgccgtg agcgagcgcc tcgagcaggt ctcgctgtcc
     1021 tgcgccgacg cgctcgccac ggtcatcgcc gccgacaccg gcgtgtcgcg cgaggacgcc
     1081 agggtcatcg ggatggccca gatctcggct cgggactggc tctcgcaggg ctcgcccatc
     1141 ccgcgggagg aggccgccgg gctggtcggc cagctcgcgt ggcggggact gggcggcttc
     1201 cccaaggtga cccgcccgga ccgggaaggt ccggagcccg ctggccgtta gggtcagtgc
     1261 cagcacgcag ctcccgtacg aggaaggtca cacccgtgga ggtcaggatc ggcgtccaga
     1321 acgtcgcccg cgagatcacg ttcgagtccg agcagacggc cgacgaggtc cgtgcggtcg
     1381 tcacggctgc gctcgccggc ggcgaggtgc tgaccctcgt cgacgacaag ggccgcctcg
     1441 tcatcgtccc gacccgggcg ctcggctacg tccacctcgg cgagtccgag aagagccggt
     1501 tcggcttcgg cacctcgtag gccgaccacg gatcgttccc aagtcgtcac ctgacgtgag
     1561 cgcgcccaat acccggcatc cgggcctcgt cgaggcagag tgggagagca ctccaccgct
     1621 ccaggcctcc cggagcgggc cgtcccatga gggttgcgcc ccgcgaccca gaaggagaac
     1681 aacacccatg tcgatcattg gaagcatcat cttcggcgcc atcattggcg ccttggctcg
     1741 ggtgatcctc cccggccgtc agaacatcag cac
//
LOCUS       DATAIX010000582         2659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_253032, whole genome shotgun sequence.
ACCESSION   DATAIX010000582 DATAIX010000000
VERSION     DATAIX010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253032"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..939
                     /locus_tag="VHM65_08590"
     CDS             100..939
                     /locus_tag="VHM65_08590"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX2705803.1"
                     /translation="MTTAASTSAAASTNRALVRVLRDSLAAQGLPADAVTLLDGGGHD
                     AARALITARGLVDLVIPRGGAALIQTVVMESTVPVIETGIGICHVYVDSAADLDKALP
                     ILLNAKTQRPSVCNAAETLLVHRALAPSFLPKALAALGGAGVQLHVADEVATYAHEAG
                     AAYGIASDADFATEWHGLEMSVRVVDDLDAALEHIRTFGSGHTEVIVTEDRGAARRFT
                     AEVDAAVVMVNASSRFTDGAEFGFGAEIGISTQKLHARGPMALPELTTTKWVVEGDGQ
                     IRT"
     gene            complement(929..1987)
                     /locus_tag="VHM65_08595"
     CDS             complement(929..1987)
                     /locus_tag="VHM65_08595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2705804.1"
                     /translation="MSLELSVVIPMFNEEEVLPLLVERLRPILDGLHTSYEVVAVDDG
                     SSDLTPALLQRFHREWRQLRVVRLRANAGHQAAISAGLVSAQGDYVATIDADLQDPPE
                     VIAEMLEVVRRERVDVVYGVRSDRSTDTAFKRLTAKAFYALIKALSGTTAHSDAGDYR
                     LMSRATVEAINALPESNRVLRFIVPAFGFPSETVEYRREERAAGTSKYPFAKMLKLSL
                     DSVTGFSLAPLRLATYLGLGGGVFAIFLGVFALIARWQGRAVAGWTSTVLIVASVGAA
                     QLLCLGLLGEYVGRMYRAMQARPTYFIAYDSLTARVGRADRQPAVEAPAVEAPAVEAP
                     AVEAPAVEAPAVEAGVTS"
     gene            2045..2233
                     /locus_tag="VHM65_08600"
     CDS             2045..2233
                     /locus_tag="VHM65_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705805.1"
                     /translation="MSAHVLPVLIHAEEKAFELPMPAIAYGLSIFGAFLLLLLILWFF
                     RNTASKLQRRDPGTGAHH"
     gene            2251..>2659
                     /gene="nadD"
                     /locus_tag="VHM65_08605"
     CDS             2251..>2659
                     /gene="nadD"
                     /locus_tag="VHM65_08605"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX2705806.1"
                     /translation="MGGTFDPIHHGHLVAASEVMALFGLDEVVFVPTGQPWQKSSSTV
                     TPAEHRYLMTVIATASNPRFSVSRVDIDRSGPTYTIDTLRDLHRQRPDAELFFITGAD
                     ALAQILSWKDIDELWELAHFIGVTRPGHVLSDAG"
BASE COUNT          404 a          948 c          919 g          388 t
ORIGIN      
        1 gccatcgggc cgcgcgcgtg cagtttctgg gtcgagatcc cgatctcggc gccgaagccg
       61 aactcggcgc cgtcggtgaa cctcgacgag gcgttgacca tgaccaccgc ggcgtcgacc
      121 tcggccgcgg cctcgaccaa ccgggcgctc gtgcgcgtgc tccgggactc ccttgccgcg
      181 caagggctcc cggcagatgc ggtgaccctg ctcgacggcg gcggccatga cgccgcgcgg
      241 gccctcatca cggcacgcgg cctcgtcgac ctcgtcatcc cccgcggcgg agccgcgctc
      301 atccagaccg tcgtcatgga gtcgaccgtg cccgtcatcg agacaggcat cgggatctgc
      361 cacgtctacg tcgactccgc cgccgatctg gacaaggcgt tgccgattct gctcaacgcc
      421 aagacccagc ggccgagcgt gtgcaacgcc gccgagacgc tgctcgtcca ccgggcgctc
      481 gccccgtcgt tcctgcccaa ggccctcgcg gcgctcggcg gggccggtgt gcagctgcat
      541 gtcgccgacg aggtggcgac ctacgcgcac gaggcgggcg cggcatacgg catcgccagt
      601 gacgcggact tcgccacgga gtggcacggg ctggagatgt cggtgcgggt ggtggacgac
      661 ctcgacgccg cgctggagca catccgcacg ttcgggagcg gccacaccga ggtcatcgtc
      721 accgaggacc gcggggcggc gcggcggttc acggccgagg tcgacgccgc ggtggtcatg
      781 gtcaacgcct cgtcgaggtt caccgacggc gccgagttcg gcttcggcgc cgagatcggg
      841 atctcgaccc agaaactgca cgcgcgcggc ccgatggccc tgcccgagct gacgacgacg
      901 aagtgggtcg tcgagggcga cgggcaaatc aggacgtgac gccggcctcg actgcgggtg
      961 cctcgacggc gggagcctcg acggcgggtg cctcgactgc gggtgcctcg accgcgggtg
     1021 cctcgaccgc gggctgccgg tcagcccgcc ccacccgtgc ggtcagcgag tcgtaggcga
     1081 tgaagtaggt cggccgggcc tgcatggccc ggtacatgcg cccgacatac tcgccgagaa
     1141 ggccgaggca cagcagctgc gccgcaccga ccgaggcgac gatgagcacg gtcgaggtcc
     1201 atccggcgac cgcccgcccc tgccaccggg cgatcagcgc gaagacgccg aggaatatcg
     1261 cgaagacccc gccaccgagt ccgaggtagg tcgcgagccg caggggagcg agggagaacc
     1321 cggtcaccga gtccagcgag agcttgagca tcttggcgaa cgggtacttc gaggtcccgg
     1381 ctgcacgctc ctcgcggcgg tactcgacgg tctcggaggg gaagccgaac gcggggacga
     1441 tgaaccgcag cacccggttg ctctccggga gcgcgttgat cgcctcgacc gttgcccggg
     1501 acatgagccg gtagtcgccg gcatcgctgt gggccgtcgt ccccgacagc gccttgatga
     1561 gcgcatagaa ggccttggcc gtgagccgtt tgaaggccgt gtccgtcgag cggtcggagc
     1621 ggacgccata gacgacgtcg acccgctcgc ggcggacgac ctcgagcatc tcggcgatga
     1681 cctcgggcgg atcctgcagg tcggcatcga tcgtcgccac gtagtcgccc tgggcgctga
     1741 cgaggccggc cgagatggcc gcctggtggc cggcgttggc gcgcagacgc acgacccgca
     1801 gctggcgcca ttcacggtgg aagcgctgga gcagggccgg ggtgaggtcc gagctgccgt
     1861 cgtcgaccgc gacgacctcg taggacgtgt gcagcccgtc gaggatcggg cgcagccgct
     1921 cgacgaggag cgggaggacc tcctcctcgt tgaacatcgg gatgacgacc gacagctcga
     1981 gactcatgtc tgccagcgta cgggcccacc cgtggctcac ctgtgcacgc gctaggctgt
     2041 gcccatgtct gcacacgtcc tgcccgtcct catccacgct gaggagaagg ccttcgagct
     2101 cccgatgccc gcgatcgcct acgggctcag catcttcggt gccttcctcc tgctcctgct
     2161 gatcctctgg ttcttccgca acaccgccag caagctccag cggcgcgacc ccggcacggg
     2221 ggctcaccac tgacggtccg actcggggtg atgggcggta ccttcgaccc catccaccac
     2281 ggtcacctgg tcgccgcctc cgaggtcatg gcgcttttcg gcctcgacga ggtcgtcttc
     2341 gtgcccaccg gccagccctg gcagaagtcg tcctccacgg tgaccccggc cgagcatcgc
     2401 tatctcatga cggtcatcgc gaccgcctcg aacccgcggt tctcggtgag ccgggtcgac
     2461 atcgaccgaa gcgggccgac ctacacgatc gacaccctgc gcgacctgca ccggcagcgt
     2521 ccggacgccg agctgttctt catcacgggc gccgacgcac tggcccagat cctgtcgtgg
     2581 aaggacatcg acgagctctg ggagctggcc cacttcatcg gcgtcacccg gcccgggcat
     2641 gtcctgtccg acgcggggc
//
LOCUS       DATAIX010000583         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_253067, whole genome shotgun sequence.
ACCESSION   DATAIX010000583 DATAIX010000000
VERSION     DATAIX010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253067"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /gene="lnt"
                     /locus_tag="VHM65_08610"
     CDS             complement(<1..1163)
                     /gene="lnt"
                     /locus_tag="VHM65_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX2705807.1"
                     /translation="MRRALLALLAGGALTLSFPGSGIWVLAPVGVGALALATRGTSIR
                     TGAGLGLLAGLACFLPTLSWTGTYVGALPWVALAILQALYVAFAGAVSSALQRGVSVA
                     GADRVRPFVVALAWVGSEALRARTPYGGFPWARIAFTQADSPLAHIAALAGAPGVTFA
                     VALAGGVLASAVAPRATLSAPTDLESPGAFSTRVGVAGSTRSRVVAMPRWRRLGVAVV
                     LVVSPLAVPLYSEGRPADVMAVQGNVPHPGLDFNAERRAVLDNHVSATTRAAAQVRAG
                     SLTQPDLVVWPENSSDIDPVRNTDAALQVNLALEAIRAPLLIGTLRIGDDRSVRNVAE
                     LYEAGRGLVAEYTKRHPVPFAEYIPNRAFFRLFSDKVDLLTRDFSAGSAPGLF"
     gene            complement(1694..>2070)
                     /locus_tag="VHM65_08615"
     CDS             complement(1694..>2070)
                     /locus_tag="VHM65_08615"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEX2705808.1"
                     /translation="MTVTHRRYVSYSHAHYAGNLVDGAYSLAAFGDVATEMCIRTDGD
                     EGLFASYSDVQFRAPVRAGDVIEIEARLVRIGSRSREMDFEVRVVGRGTPGRGEAAAA
                     LLDPPIVATTARGVVVVPPRAG"
BASE COUNT          325 a          732 c          755 g          258 t
ORIGIN      
        1 tcgaagaggc ctggcgccga gccggccgag aagtcgcggg tcagcaggtc gaccttgtcg
       61 ctgaacagcc ggaagaacgc ccggttcggg atgtactcgg caaaggggac cgggtggcgc
      121 ttggtgtact ccgcgaccag accgcgtccg gcttcgtaga gctccgcgac attgcgcacc
      181 gagcggtcgt ccccgatccg cagggtgccg atgagcaagg gggcgcggat cgcctcgaga
      241 gccaggttga cctggagcgc agcgtcggtg ttgcggacgg ggtcgatgtc cgaggagttc
      301 tccggccaga cgaccaggtc tggctgggtc agcgacccgg cccgcacctg agcggccgcc
      361 cgggttgtcg cgctcacgtg gttgtcgagg acggcgcggc gctcggcgtt gaagtccaga
      421 ccggggtgcg ggacgttgcc ctgcacggcc atgacgtctg ctgggcgacc ctcgctgtac
      481 agcggcaccg ccagcggtga caccactagc accaccgcta cacccaaccg gcgccagcgc
      541 ggcatcgcca cgacgcggga gcgggtggag cccgcgacgc cgacccgggt cgagaaagct
      601 ccaggactct ccagatctgt gggggccgag agggtggccc ggggagccac cgcggaggcg
      661 agcactcccc cggccagggc caccgcgaag gtcacgcccg gagccccggc cagggccgcg
      721 atgtgggcca atggggagtc ggcctgggtg aaggcgatcc gggcccaggg gaagccaccg
      781 tatggcgtgc gcgcacgcag cgcctctgac cccacccacg cgagggcgac gacgaacgga
      841 cggacgcggt cggccccggc aaccgacacg cctcgctgca gtgcggacga cacggcgccg
      901 gcgaacgcga cgtacagggc ttgcaggatc gcgagcgcaa cccagggcag cgctccgaca
      961 taggtccccg tccacgacag ggtcggcagg aagcaggcca gcccggccag gagccccaga
     1021 ccggccccgg tgcggatcga ggtcccgcgc gttgcgagcg cgagcgcgcc cacaccgaca
     1081 ggggcgagca cccagatgcc ggagccgggg aacgacagcg tgagcgcgcc cccagccagc
     1141 agggcgagga gggctcggcg cacggagaca cggtagctgt cccggggcct gaactcacac
     1201 tgacgcgcct gccagcctgc cggccctgcc ggcccgtcgg taacggcccc ggtccaggcg
     1261 cagcgttgag ggaacagaag acccgccccg ccgttggtac cacccacggg tccggtcgga
     1321 tgccgggccg cgggcgttgt ctactaggcg tgacgggtct tccgccgttc accccctgca
     1381 ttggcgccgt gagagagcca ccggacgggc ctggaacgcg cggtggctgc gcgcacatgc
     1441 cacggtgtaa cccctccgga gctgtcccac ggccacccgg atgatggcct gttggtgaac
     1501 aacccgggtc aaatttaccc tcggcgttca ccctgtcaac tggcctctga cctgcgcaga
     1561 cgcgcgtttt cgctggtcag gtcgggtgtt cggacctgtc cggatgagcc tcttgcccgg
     1621 tcgccccggc gtgtcgctcg cgacacgccg gggcgaccgg cggagcgctg gccgacgtac
     1681 ccagggtaga cgctcacccg gcgcgtgggg gcacgacgac gacgccgcgt gcggtggtgg
     1741 ccacgatcgg cgggtcgagc agcgcggccg cagcctcccc ccgcccgggc gtcccgcgtc
     1801 ccacgacgcg cacctcgaag tccatctcgc ggctgcgcga cccgatccgg accaggcggg
     1861 cctcgatctc gatcacgtcg cccgcgcgca ccggggcccg gaactgcacg tcgctgtatg
     1921 acgcgaacag cccctcgtcg ccgtcggtgc ggatgcacat ctcggtggcg acatcgccga
     1981 acgcggccag cgagtaggca ccgtcgacga ggttgcccgc gtagtgggcg tggctgtagg
     2041 acacgtagcg gcgatgcgtg accgtcatac
//
LOCUS       DATAIX010000584         2621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_194147, whole genome shotgun sequence.
ACCESSION   DATAIX010000584 DATAIX010000000
VERSION     DATAIX010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194147"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1838
                     /locus_tag="VHM65_08620"
     CDS             <1..1838
                     /locus_tag="VHM65_08620"
                     /inference="COORDINATES: protein motif:HMM:NF004189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HEX2705809.1"
                     /translation="VRDNGRGIPTDIHPNEGVSAVELVLTQLHAGGKFGGGGYKVSGG
                     LHGVGSSVVNALSSRLDVCVRQRGHAYRMSFDHGVPVAPLAEMESTDRTGTTVTYWAN
                     AEIFETVDYDFETVRARFQQMAFLNKGLTINLVDERIETVIADDEVDLDELTADVVEA
                     SADEDDEPSAPSATSGKVKKVTYRYDGGLVDYVRHLTSSKRSEPVHPDIIAIEAEDEK
                     RSLSLEIAMQWTTAYSESVHTYANTINTHEGGTHEEGFRAALTRLINEFARKHNQLKD
                     KDENLTGDDVREGLTAVISVKLAEPQFEGQTKTKLGNSEVKGFVQSALTEEFGHWLGT
                     HPREGRDIVAKAISASAARLAARKAREATRRKSPLESGGLPGKLRDCQSKDPSLSEVF
                     IVEGDSAGGSAVQGRNPTTQAILPIRGKILNVEKARLDKVLANNEVQALISAFGTGIG
                     EEFDLTRARYHKIVLMADADVDGMHIRTLLLTLLFRFMRPLIEAGYVYLAQPPLYRIK
                     WSNAPHEFAFSDRERDGLMAIGLAKGWRVPKDNSTQRYKGLGEMNYEELWDTTMNPDT
                     RTLLQVTLADAAAADEIFAILMGEDVESRRGFIQRNARDVRFLDI"
     gene            1909..>2621
                     /locus_tag="VHM65_08625"
     CDS             1909..>2621
                     /locus_tag="VHM65_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX2705810.1"
                     /translation="MTDRIEPVDLNTEMQRSYIEYAMSVIVSRALPDVRDGLKPVHRR
                     VLYAMYDGGYRPDRGYNKCSRVVGDVMGQYHPHGDSAIYDALVRLVQPWSLRYPLVDG
                     QGNFGSPGDDPAAAPRYTECRMAQLSMEMVRDIDEETVDFVPNYDGKTMQPSVLPSRF
                     PNLLINGSSGIAVGMATQIPPHNLREVAEGALWYLANPEVGRAELLEKLLTVVKGPDF
                     PTGALIMGRRGIEDAYRTGR"
BASE COUNT          470 a          918 c          862 g          371 t
ORIGIN      
        1 gggtccgtga caacggccgc gggatcccca ccgacatcca cccgaacgag ggggtgtcgg
       61 cggtcgagct ggtcctgacc cagctgcacg ccgggggcaa gttcggcggg ggcggctaca
      121 aggtgtccgg agggctgcac ggggtcggct cctccgtggt caacgcgctg tctagccggc
      181 ttgacgtctg tgtgcgccag cggggccacg cctaccggat gagcttcgac catggggtgc
      241 ccgtcgcccc gctggccgag atggagtcca ccgaccgcac cgggacgacg gtcacctact
      301 gggccaacgc ggagatcttc gagacggtcg actacgactt cgagacggtc cgggcgcggt
      361 tccagcagat ggcgttcctc aacaagggcc tgaccatcaa cctcgtcgac gagcggatcg
      421 agacggtcat cgccgacgac gaggtcgacc tcgacgagct gaccgccgac gtcgtcgagg
      481 ccagcgccga cgaggacgac gaacccagtg cgcccagcgc cacgagcggc aaggtcaaga
      541 aggtcaccta ccggtatgac ggcgggctgg tcgactacgt ccgccacctc acctcgtcca
      601 agcgctccga gccggtccac ccggacatca tcgcgatcga ggccgaggac gagaagcgct
      661 cgctgtcgct ggagatcgcg atgcagtgga cgacggccta ctccgagtcc gtccacacct
      721 acgccaacac gatcaacacg cacgagggcg gcacgcatga ggaggggttc cgcgcggcgc
      781 tgacccggct catcaacgag ttcgcgcgca agcataacca gctcaaggac aaggacgaga
      841 acctcaccgg cgacgacgtc cgcgaggggc tcaccgccgt catctcggtc aagctcgcgg
      901 agccccagtt cgagggccag accaagacca agctcggcaa ctccgaggtc aagggcttcg
      961 tccagagcgc cctgaccgag gagttcgggc actggctcgg gacccacccg cgggagggcc
     1021 gcgacatcgt cgccaaggcg atctcggcct cggcggcgcg cctcgccgca cgcaaggccc
     1081 gggaggcgac ccggcgcaag agcccgctcg agtccggtgg cctgcccggc aagctgcgcg
     1141 actgccagag caaggacccg tccctgtcgg aggtcttcat cgtcgagggc gactcggccg
     1201 gcggctcggc cgtgcagggc cgcaacccga cgacccaggc gatcctgccg atccgcggca
     1261 agatcctcaa cgtcgagaag gcccggctcg acaaggttct cgccaacaac gaggtccagg
     1321 cactgatctc ggccttcggc acggggatcg gcgaggagtt cgacctcacc cgggcccgct
     1381 atcacaagat cgtgctcatg gccgatgccg acgtcgacgg gatgcatatc cggaccctgc
     1441 tgctcacgct gctcttccgg ttcatgcgcc cgctcatcga ggccggctac gtctacctcg
     1501 cccagccccc gctctaccgg atcaagtggt ccaacgcgcc gcacgagttc gccttcagcg
     1561 atcgcgagcg tgacggtctc atggcgatcg gcctggccaa gggctggcgg gtgccgaagg
     1621 acaactcgac ccagcgctac aagggtctcg gtgagatgaa ctacgaggag ctctgggaca
     1681 ccacgatgaa cccggacacc aggacactgc tgcaggtcac cctcgccgac gccgccgccg
     1741 ccgacgagat cttcgcgatc ctcatgggtg aggatgtcga gtcccgtcgc ggcttcatcc
     1801 agcgcaacgc ccgcgacgtg cggttcctgg acatctgacg cagcgcaccg tcatacaccc
     1861 agaaacattg ataggtaagg gaaccctgtg actgagcagc cgccgaccgt gaccgaccgg
     1921 attgagccgg tcgacctcaa caccgagatg cagcgctcgt acatcgagta cgccatgtcc
     1981 gtcatcgtgt cccgggccct gcccgacgtc cgggacggtc tcaagccggt gcaccggcgc
     2041 gtgctctatg ccatgtatga cggtgggtac cgtcccgacc gcggctacaa caaatgttcg
     2101 cgggtcgtcg gtgacgtgat gggccagtac cacccgcacg gtgactcggc gatctacgac
     2161 gcgctggtgc gcctcgtcca gccgtggtcg ctgcgctacc cgctcgtcga cgggcagggc
     2221 aacttcggct cgccgggcga cgacccggcg gcggcgccgc gctacaccga gtgccggatg
     2281 gcgcagctgt cgatggagat ggtccgcgac atcgacgagg agaccgtcga cttcgtcccg
     2341 aactacgacg gcaagacgat gcagccgtcg gtcctgccga gccggttccc gaacctgctc
     2401 atcaacggct cttccgggat cgcggtgggg atggccaccc agatcccgcc gcacaacctg
     2461 cgcgaggtcg ccgagggcgc cctgtggtac ctcgccaacc ccgaggtcgg ccgcgcggag
     2521 ctgctcgaga agctgctcac cgtcgtcaag ggccccgact tcccgacggg cgcgctcatc
     2581 atggggcgca ggggcatcga ggacgcctac cggaccgggc g
//
LOCUS       DATAIX010000585         1769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_134081, whole genome shotgun sequence.
ACCESSION   DATAIX010000585 DATAIX010000000
VERSION     DATAIX010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134081"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VHM65_08630"
     CDS             <1..233
                     /locus_tag="VHM65_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705811.1"
                     /translation="AWAATVAVVAAYRPAQPAVPGPPPRGDLEVGEAFARAAAHGGEH
                     VIKLADTALSSFERTGNPLGLAAVDTAIRLEA"
     gene            complement(248..958)
                     /locus_tag="VHM65_08635"
     CDS             complement(248..958)
                     /locus_tag="VHM65_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705812.1"
                     /translation="MTPFFEPATPPDEPEPEPHYVVFPGQPPENVVPARLDWDAEILR
                     EPDTVLVLTDVLAFPQGFSYRVRAWLRPGSEHHAEGGPRHEHVDFAPVTGWLLDGSVK
                     IGAAWETDGASGGDDPFAPGDPAAPRQSPCGGSGGGLTADTGSWVYPLPEGHSLQLVF
                     AWDARGIPETYVDVDLAELGAAAGRAEVLWPIPESPHEQGWFAYAPLQSGSAVRGAPS
                     TAADTSDDTSEDPDGHAS"
     gene            1052..>1769
                     /gene="recO"
                     /locus_tag="VHM65_08640"
     CDS             1052..>1769
                     /gene="recO"
                     /locus_tag="VHM65_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX2705813.1"
                     /translation="MPLYRDEAVVLRTHKLGEADRIITLLTRTRGKVRTVAKGVRRTT
                     SRFGSRLEPFMAVDVMCYEGRNLDTVTQAETIAPYGEAICRDYAAFTAASAIVETADR
                     LTEEGAPATQQYLLLSGALRSLATGEHDPGLVLDAYLLRSLAVAGWAPSFHDCARCGA
                     VGPHGAFNAAAGGAVCPSCRPLGSAAPAALTLELLAALLSGDWPLADAADGRSRREGS
                     GLVAAFLQWHLERQVRSLEHV"
BASE COUNT          227 a          648 c          653 g          241 t
ORIGIN      
        1 ccgcgtgggc ggccaccgtc gccgttgtcg cggcctaccg gcccgcgcag ccagcagtgc
       61 cgggtccccc gcccaggggc gacctcgagg tgggcgaggc attcgcccgg gccgcggccc
      121 atggcggcga gcacgtcatc aagctcgccg acaccgcact gagctcgttc gagcgcacgg
      181 gcaacccgct cggtctggcg gccgtcgaca cggccatccg gctggaggcg tgaccggcgc
      241 agcgggccta cgacgcgtgg ccgtcagggt cctcgctcgt gtcgtcggag gtgtccgcgg
      301 cggtcgatgg cgcaccgcga accgccgatc cgctctggag gggggcgtag gcgaaccatc
      361 cctgctcgtg gggcgactcc gggatcggcc agagaacctc ggcccggccg gccgcggcgc
      421 ccagctcggc gaggtcgacg tcgacatacg tctcggggat gccccgcgcg tcccaggcga
      481 agacgagctg caaggaatgc ccctcgggca ggggatacac ccagcttccg gtgtccgcgg
      541 tgagcccgcc accgcttccg ccgcacggtg actgccgggg tgcggccgga tcgcccggcg
      601 cgaacgggtc gtctccgccg ctcgctccgt cagtctccca ggcggcaccg atcttcaccg
      661 acccgtcgag cagccagccg gtcacgggcg cgaagtcgac gtgctcatgg cgggggcctc
      721 cttcggcgtg gtgctccgag cccggacgca accacgcacg gacgcggtag ctgaagccct
      781 gggggaaggc cagcacgtcg gtgaggacca ggacggtgtc gggctcgcgc agtatctcgg
      841 cgtcccagtc gagacgggcg gggacgacgt tctccggagg ctgcccgggg aacacgacgt
      901 agtgcggctc gggctcaggt tcgtccggcg gcgtggcagg ctcgaagaaa ggcgtcacac
      961 accgacggta gcccacgccg atccggagtg cgcggggtcc tggccgcgcg gcataccccc
     1021 ggccgggtcc gcgcgggggg acaatggcgc ggtgcccctc taccgtgacg aggcggtcgt
     1081 cctgcgcacc cacaagctgg gtgaggccga ccggatcatc acgctgctca cccgcacccg
     1141 cggcaaggtc cgcacggtcg ccaagggggt gcggcgcacc acgtcgcgct tcgggtcccg
     1201 gctggagcca ttcatggccg tcgacgtcat gtgctacgag gggcgcaacc tcgacacggt
     1261 gacccaggct gagacgatcg ccccgtatgg cgaggcgatc tgccgcgact atgccgcctt
     1321 cacggcggcg tcggcgatcg tcgagacggc cgaccggctc accgaggagg gcgcgcccgc
     1381 gacccagcag tacctcctgc tttccggtgc cctgcgctca ctggcgaccg gggagcatga
     1441 tcccgggctc gtcctcgatg cctacctgct tcgctcgctg gccgtcgccg ggtgggcgcc
     1501 gagcttccac gactgcgcca gatgtggtgc ggtggggccc cacggggcgt tcaacgccgc
     1561 cgccggcgga gccgtctgcc cgtcgtgccg cccgctgggc tcggccgcgc cggctgcgct
     1621 gaccctcgag ctgcttgccg ccctgctctc gggggactgg cccctcgccg acgccgccga
     1681 cgggcgctcc cggcgcgagg ggagtgggct cgtcgccgcc ttcctccagt ggcacctgga
     1741 gcgccaggtg aggtccctgg agcatgtgg
//
LOCUS       DATAIX010000586         1981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_194198, whole genome shotgun sequence.
ACCESSION   DATAIX010000586 DATAIX010000000
VERSION     DATAIX010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194198"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1652)
                     /gene="ptsP"
                     /locus_tag="VHM65_08645"
     CDS             complement(<1..1652)
                     /gene="ptsP"
                     /locus_tag="VHM65_08645"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX2705814.1"
                     /translation="MSARLEGLGVSPGTASGPIARLGLPPRPPSDEVPGASIEIELER
                     VGEALEAVATSLEERATTAPGEAAAVLGATAMMARDPGLLSAVKGLLEQGRATAHSVD
                     AAIEGFCDVLAASGGYLAERVADLRDVRNRALAVLLDEPMPGIPHPGHPFVLVGQDLS
                     PADTSTLDPAEVLALITELGGPTSHTAILAKGLGLPAVVQCSGALDLADGTLVVVDGS
                     SGAVVIDPDQKTLAEAQSRAEARRMLTASRYGPGATADGTGIQRLVNIATVDDAKRAS
                     SVDSEGVGLFRTEGLYLGRQTAPTVEEQEALYREVFGAFGTRKIVVRTLDAGADKPLA
                     FVQHGDEENPALGVRGLRVSRRYPDLLQTQLEALGRAAKASTTTVWVMAPMVSTAREA
                     RDFSTAVKAHGLAVAGAMIEVPAAALRAREVLREVDFVSIGTNDLAQYTFAADRMQGE
                     LADLLDPWQPALLDLIASVGAAGATTSTPVGVCGEAAGDALLACVLVGLGVRSLSMAP
                     GLVAAVRLVVGAHSLARCVEMATAARLAPDAVSARAAARELA"
     gene            complement(1654..>1981)
                     /locus_tag="VHM65_08650"
     CDS             complement(1654..>1981)
                     /locus_tag="VHM65_08650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX2705815.1"
                     /translation="PARSAARKEVFMPERHVVVGSSVGLHARPAALFTQAAAKAPVPV
                     TIAKVGGDPVDARSILLVLTLGVAHADEVVLSAEGDGADAALDSLAALLEQDLDAPSA
                     PSSAGA"
BASE COUNT          282 a          739 c          691 g          269 t
ORIGIN      
        1 tccgccagtt ccctggccgc agcccgtgcg ctgacggcat ccggtgccag ccgcgctgcg
       61 gtcgccatct cgacgcaacg agccagcgag tgggcgccga ccaccagtcg gaccgccgcc
      121 accagccccg gggccatcga caggctccgc acgcccaggc ccaccagcac acaggccagc
      181 agcgcgtcgc cggccgcctc gccgcagacc ccgaccgggg tggacgtcgt ggccccggcc
      241 gccccgaccg acgcgatcag gtcgagcagg gccggctgcc acgggtcgag cagatcggcc
      301 agttcgccct gcatccggtc ggcggcgaag gtgtactgcg ccaggtcgtt ggtgccgatg
      361 ctgacgaagt ccacctcgcg gagcacctca cgggcccgca acgcggcggc cggtacctcg
      421 atcatggctc cggccacggc caagccgtgt gccttcaccg cggtcgagaa gtccctggcc
      481 tcgcgagcgg tggagaccat cggagccatc acccagaccg tcgtggtcga ggccttcgcc
      541 gcccgcccga gcgcctcgag ctgggtctgg agcaggtcgg gataacgacg cgagacccgc
      601 aggccgcgca caccgagggc cgggttctcc tcgtccccgt gctggacgaa ggccaagggt
      661 ttgtcggcac cggcatcgag ggtgcgcacc acgatcttcc gggtgccgaa ggcgccgaac
      721 acctcacggt agagcgcctc ctgttcctcg accgtgggcg cggtctgtct gccgagatag
      781 agcccctcgg tgcggaacag cccgacaccc tcgctgtcga cgctcgaggc gcgcttcgcg
      841 tcgtccacgg tggcgatgtt caccaggcgc tggatcccgg taccgtcggc cgtcgcaccc
      901 gggccgtacc tggacgccgt gagcatccgc cgggcctcgg cccgcgactg cgcctcggcc
      961 agggtcttct ggtccggatc gatcaccacg gcgccggagg agccgtcgac caccaccagg
     1021 gtgccgtccg cgaggtcgag cgcaccggag cactggacca ctgcgggcag tcccagcccc
     1081 ttggccagga tcgcggtatg gctggtcggg ccacccagct cggtgatcag tgccagcacc
     1141 tccgccgggt ccagcgtgga ggtgtcggcg ggggacaggt cctgcccgac cagcacgaac
     1201 gggtgccccg gatgcgggat ccctggcatc ggctcgtcca gcagcaccgc cagcgcacga
     1261 ttgcggacgt cgcgcaggtc ggccacccgc tcggccagat agccacccga ggcggcgagc
     1321 acatcgcaga agccctcgat cgcggcgtcc acgctgtgtg ctgtcgccct gccctgctcg
     1381 agcaaccctt tgacggcgct gagcaggccc gggtcccgag ccatcatcgc ggtagcgccc
     1441 agcacggccg cggcctcgcc gggtgccgtg gtggcgcgct cctcgagcga ggtggccacc
     1501 gcttcgagtg cttcgccgac ccgttcgagt tcgatctcga tgctcgcccc gggcacttcg
     1561 tccgacggag gcctgggcgg cagtcccaga cgtgcgatcg ggccactcgc ggtaccgggg
     1621 ctgactccca gcccctccaa ccgggcagac atctcaggct ccggcggacg acggtgccga
     1681 cggtgcgtcg aggtcctgct cgagcagggc ggcgagcgag tcgagggccg cgtcggcacc
     1741 gtcgccctcc gcgctgagca cgacctcgtc ggcgtgagcg acgcccaggg tgagcacgag
     1801 gaggatgctt cgggcgtcga ccgggtcccc acccaccttg gcgatggtca ccggtacggg
     1861 cgctttggcc gccgcctgtg tgaacagtgc cgcggggcgg gcgtgcagac ccacgctgga
     1921 tccgacgacg acatgacgct cgggcatgaa gacctccttg cgggctgcgc tgcgcgcagg
     1981 a
//
LOCUS       DATAIX010000587         3565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_283555, whole genome shotgun sequence.
ACCESSION   DATAIX010000587 DATAIX010000000
VERSION     DATAIX010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283555"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            602..1126
                     /locus_tag="VHM65_08655"
     CDS             602..1126
                     /locus_tag="VHM65_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705816.1"
                     /translation="MVLSRSAGRFAVAFLALGIVARGWMRLITTEPEFSWSGALDIVG
                     AFVVFGVACGLVRGARRSGRSRVWRLLAPLCLGIGVGAGALLVPGVALTAWATSGRGP
                     RWVRWLVPGILVAVAALGVAQDEGADLGRLAITVTGLWALTLGLGLGAREFLMRWPGR
                     AHGVSERALRVVVP"
     gene            1213..2058
                     /locus_tag="VHM65_08660"
     CDS             1213..2058
                     /locus_tag="VHM65_08660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2705817.1"
                     /translation="MLRALTPVRALSESDRDEALALCAQDPPANVFVAARIHEGMLRS
                     YPGALLGYRAEGTLTSLCWSAANLVPVGADTAALDQFAIRVRRWRHRCASIFGPAELV
                     VPLWDRLRDPWGAPRALRPRQPLMLTSTRPSQLGVELHPGVRPASESEVDRVLPAAAA
                     MFTAEIGYPPYRGSAQSYRSMLLELIRRGHTFVWVDRGEVVFKADVGSTALGVAQIQG
                     VWLTPRLRGQGLGVAAMGAVTEQVMATIAPQVSLYVNDFNAPAIAVYRRVGFTDAGTF
                     STVLL"
     gene            2059..2913
                     /locus_tag="VHM65_08665"
     CDS             2059..2913
                     /locus_tag="VHM65_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2705818.1"
                     /translation="MSTLEWGPLQESDLPGLGSLARACLRKDGGLPQLATEAMLRHYF
                     LTGVSIGGRDETGELVAVASVFWDSQGRRTATGMVHPSARLQGHGDSLVAWCRDHSPG
                     VNLKVVAESMSPEAEALFARNGLRRTFAETVMCHSLGHIPRIALPDGLRTHPFGEDTA
                     LAFHTAYRLAFAERPGFPDTPAEKWLGWVRDDPGFRPEQSRVALAADGSPAGFVILVD
                     AWIDQVGVVPQWRGRRLGAHLVARSLRALKNAGADSVWLNVNVDNPARILYERLGFEV
                     RGTRARYE"
     gene            2979..>3565
                     /locus_tag="VHM65_08670"
     CDS             2979..>3565
                     /locus_tag="VHM65_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEX2705819.1"
                     /translation="MALRMSTLFLRTLREDPADAELPSHRLLVRAGYIRRTSPGIYTW
                     LPLGLRVLRNVEAIVRDEMEAIGAQELLFPALLPKEPFESTGRWAEYGDNIFRLQDRK
                     GAGYLLGPTHEEMFTLTVKDLFSSYKDLPLSLYQIQTKYRDEARPRGGLLRGREFVMK
                     DSYSFDVDEAGLDKAYDLHREAYVRIFDRLGFHYV"
BASE COUNT          425 a         1260 c         1288 g          592 t
ORIGIN      
        1 tgaccgggca gctcaagggc ggcggcccga cggacggaag aggggttcat catgacgact
       61 tccatgcacg cgaagaccac agcgactatg cgactactgg ctgccggggc gacggccatg
      121 ggcctggtct gggccggggc acttccggcg tcggcggcct gtccgacgcg actcgtcttc
      181 tcctcgcgtt tgaccgatct ggctccagcg acgtccgagc ccttcgacgg ggcccacgct
      241 cacgcggtgc ttcggctggg ccgggaccga acccgggtcg tgctcagcgt cagagggatc
      301 gagtcgtcgg gtgcggtatg acgtacggtg ctcacctaca cgtgggcccg tgcgtggccg
      361 gcgatggcgc ggcggctggt ccccactcca accagtccgt cgtcgacggg gtggtcccac
      421 aggtggtcga cgcgacgacc gaggtgtggc tcgacttcac cgtgcgaccc aacgggtcgg
      481 cgcgttcggt agcagtcgtt ccgttcgtgc ccacatccgg cacccgctcc atcgtcattc
      541 acgctatgcc gaccgacccg gctggggctg cgggtgcgcg gcttgcctgc ctgccggtgg
      601 tgtggtcctg agccggtccg ccgggcgctt cgcggtggcc tttctcgccc ttgggatcgt
      661 cgcgcggggc tggatgcgcc tcatcacgac cgagcccgag ttcagctggt caggcgccct
      721 cgacatcgtc ggagcgttcg tggtcttcgg ggtggcctgc ggtctcgtgc gcggtgcgcg
      781 gcggtcggga cggtcgaggg tgtggcgcct cctcgcgccg ctgtgtctcg ggatcggagt
      841 gggggccggg gcgctgctcg tgcccggggt cgccctgacg gcgtgggcga cctcaggccg
      901 ggggcctcga tgggtgcgct ggctcgttcc gggcatcctc gtcgcggtcg ccgccctcgg
      961 cgtcgcgcag gacgagggcg cagatctggg ccgcctcgcc atcacggtca cgggtctgtg
     1021 ggccctcacc ctgggcctcg ggcttggcgc gcgcgagttc ctgatgcgct ggccgggccg
     1081 cgctcacggg gtgagcgaac gggccctgcg cgtcgtggtg ccctgacccc gctggttcac
     1141 acctcccgcc tgggcgggga gcctggcgcg cgtcgatgtg acagcacggg ccctggcggg
     1201 gaggatcagt gtgtgctacg cgctctcacg cccgtgcgtg ccttgtccga gagcgaccgg
     1261 gacgaggcac tggcgctgtg cgcgcaggac ccgccggcca acgtgttcgt ggcggcccgg
     1321 atccacgagg gcatgctgcg cagctatccc ggggcactgc tcgggtaccg ggccgagggc
     1381 acgctcacgt ccctgtgctg gtcggccgcc aacctcgtgc cggtgggtgc cgacaccgcc
     1441 gccctcgacc agttcgccat ccgggtgcgg cgctggcgcc atcggtgcgc ctcgatcttc
     1501 ggtccggccg agctcgtcgt cccgttgtgg gaccggctcc gggacccttg gggcgcgccc
     1561 cgggcgctac gcccccgcca gccgctgatg ctcacctcga cccgtccgtc ccagctcggg
     1621 gtcgagctgc atcccggcgt gcgccccgcc agcgagagcg aggtcgaccg ggtgctgccc
     1681 gcggcggcag cgatgttcac cgcggagatc ggctacccgc cataccgtgg gtcggcgcag
     1741 tcctaccggt cgatgctgct cgagctcatc cgccgcgggc acaccttcgt ctgggtcgac
     1801 cggggagagg tcgtcttcaa ggccgatgtc ggttcgaccg cgctcggggt ggctcagatc
     1861 cagggggtgt ggctcacccc ccggctgcgc gggcagggtt tgggtgtcgc ggccatggga
     1921 gcagtgaccg agcaggtcat ggcgacgatc gccccgcagg tgtcgctcta tgtcaacgac
     1981 ttcaacgccc cggcgatcgc cgtctaccga cgggtggggt tcaccgacgc gggcaccttc
     2041 tcgaccgtgt tgctctaggt gtcgacgctg gagtggggtc cgctccagga gagcgacctg
     2101 cccgggctcg gctccctcgc ccgggcctgc ctgcgcaagg acggcgggct gccgcagctg
     2161 gccaccgagg cgatgctgcg gcactacttc ctcaccggcg tctcgatcgg cggacgggac
     2221 gagaccggtg agctggtcgc ggtggcctcg gtgttctggg actcccaagg gcggcgaacg
     2281 gcgaccggca tggttcatcc ctccgcgcgg ctgcaggggc acggggactc gctcgtggcc
     2341 tggtgccggg accattctcc gggggtgaac ctcaaggtcg tcgccgagtc gatgtcgccc
     2401 gaggccgagg ccctctttgc gcgcaacggg ctgcggcgga ccttcgccga gacggtgatg
     2461 tgccactcgc tcgggcacat cccccggatc gccctccccg atgggttgcg cacccatccg
     2521 ttcggtgagg acacggcgct cgccttccac acggcgtacc ggctcgcctt cgccgagcgt
     2581 cccgggttcc ccgacacccc ggccgagaag tggctcggct gggtgcgtga cgatccgggt
     2641 ttccggccgg agcagtcccg ggtggcgctc gcggcggacg ggtcgccggc gggcttcgtc
     2701 atcctcgtcg acgcgtggat cgaccaggtc ggggtcgtgc cccagtggcg gggccggcgc
     2761 ctcggtgccc acctggtggc ccgctcgctc cgggcgctga agaacgccgg ggccgactcg
     2821 gtctggctca acgtcaacgt cgacaacccc gcgcggatcc tctacgagcg gctggggttc
     2881 gaggtgcgcg gcacccgggc gcgctacgag tagcggtagg ccgcccccgt ccgtcctcgg
     2941 tatcctgcac tggtccgtcc catctcgagg agacccccgt ggccctgcgt atgtcgaccc
     3001 tgttcctgcg gactctgcgc gaggacccgg cggacgccga gctgccgagc caccgcttgc
     3061 tcgtgcgcgc cggctacatc cggcgcacct ccccggggat ctacacgtgg ctgccgctcg
     3121 ggctgcgggt gctgcgcaac gtcgaggcga tcgtccgcga cgagatggag gcgatcggcg
     3181 cccaggagct gctcttcccc gcactgctgc ccaaggagcc cttcgagtcg accgggcgct
     3241 gggccgagta cggcgacaac atcttccgtc tgcaggaccg caagggcgcc ggctacctgc
     3301 tcggcccgac ccacgaggag atgttcaccc tcaccgtcaa ggacctgttc agctcctaca
     3361 aggacctgcc actctcgctc taccagatcc agacgaagta ccgcgacgag gcgcggcccc
     3421 ggggagggct gctgcgcggg cgcgagttcg tcatgaagga ctcgtactcc ttcgacgtcg
     3481 acgaggccgg cctcgacaag gcctacgacc tgcatcgcga ggcctacgtg cggatcttcg
     3541 accggctcgg cttccactac gtcat
//
LOCUS       DATAIX010000588         2625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_134407, whole genome shotgun sequence.
ACCESSION   DATAIX010000588 DATAIX010000000
VERSION     DATAIX010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134407"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VHM65_08675"
     CDS             complement(<1..237)
                     /locus_tag="VHM65_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705820.1"
                     /translation="MLTSSWRPARIRAIVVLAAAPALAACTGADRVGSPPTASSAAAG
                     STTTTSQLPSARPVGDLDELATGLAVPWSVGFLPD"
     gene            320..1402
                     /locus_tag="VHM65_08680"
     CDS             320..1402
                     /locus_tag="VHM65_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705821.1"
                     /translation="MTQTLVAIGTRKGLWLARSDDRRAWTLDPPHFLMSEVASVAIDT
                     RSEGPRLLVGMMSFHWGPTVARSDDLGATWADTEQGAIRFPEDTGAALARVWALAADP
                     HDEEVVWAGTEPHGLWRSADRGETFDLVRGLWDHPHRASWGAGAGGGAIHTVLPDPAD
                     AASMVVAMSTGGVYRTLDSGRTWNASNTGIKASFMPDPWPEFGQCVHKVARDSGSRDR
                     LYAQNHHGVYRSDDGGGSWTSIADGLPTDFGFAVATHPSRSETVFLVPITADRERIPP
                     GAQLQVQRSTDAGDTWTTLTAGLPATSYTDVLRDALALDDADPAGVYVGTRNGEVYAS
                     LDEGDSFTCIATQLPDVLCVRAATVG"
     gene            1395..1685
                     /locus_tag="VHM65_08685"
     CDS             1395..1685
                     /locus_tag="VHM65_08685"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX2705822.1"
                     /translation="MAEAHVLVPSALREYVGGASALAVAVPAQGSPLAEVLGAALGPW
                     PMAQRRVRDERDEIRTHVNVFVDGEDVKRGAGLATTVRPGSRVHILASVAGG"
     gene            1957..2481
                     /locus_tag="VHM65_08690"
     CDS             1957..2481
                     /locus_tag="VHM65_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX2705823.1"
                     /translation="MSTGAADRTLILLRHAKAEHVWGKPDHDRELTTRGQRDARAVGA
                     WLHEHGLICDVVLCSTSMRTRQTWEGVEAGGGATEVIDYDPLIYGGSTDRLLRVIRDG
                     AGTADTLMVVGHAPSIPDLVSMLSEGEGSMQAHQALAAGFPTSGVAVLSYRGAWSALD
                     YGTASLERFHVCRG"
BASE COUNT          360 a          926 c          974 g          365 t
ORIGIN      
        1 atccgggagg aagccgaccg accacggcac ggccaggccg gtggcgagct cgtcgaggtc
       61 acccacgggt cgggctgacg ggagctgcga ggtcgtcgtg gtggagccgg cggccgccga
      121 ggacgccgtc gggggcgacc ccacccgatc ggctccggtg catgcggcga gggcgggtgc
      181 ggcggcgagc acgacgatgg cgcggatccg cgcgggtcgc cacgatgagg tgagcacgtc
      241 ccattctgga cctgccccgg tccaacgcgg ccgcacggga ccgttgtcct tgtcggtact
      301 cgggggcaca ctcatccgta tgacgcagac actcgtcgcg atcggcactc gcaagggcct
      361 gtggctggcc cgcagcgacg accggcgcgc ctggacactc gatcccccgc acttcctcat
      421 gagcgaggtg gccagcgtcg cgatcgacac gcgcagcgag gggccgcggc tcctcgtggg
      481 gatgatgtcc ttccactggg gaccgacggt ggcgcgcagt gacgacctcg gcgcgacctg
      541 ggccgacacc gagcaggggg cgatccgctt cccggaggac accggggcgg ccctggcccg
      601 ggtctgggcg ctcgccgccg acccgcacga cgaggaggtg gtgtgggcgg gcaccgagcc
      661 ccatgggttg tggcgcagcg ccgaccgggg cgagaccttc gacctcgtcc ggggtctgtg
      721 ggaccacccg caccgggcgt cctggggggc cggtgccggt gggggagcga tccacacggt
      781 gctgcccgat cccgccgacg ccgcttcgat ggtcgtcgcc atgagcacgg gcggggtcta
      841 ccggacgctc gactcgggac ggacgtggaa cgcctccaac acggggatca aggcctcctt
      901 catgcccgac ccctggccgg agttcggcca gtgcgtccac aaggtcgccc gcgacagcgg
      961 ctcgcgggac cggctctatg cccagaacca ccacggggtc taccggtccg acgacggcgg
     1021 tgggtcatgg acctcgattg ccgacgggct gccgaccgac ttcggctttg ccgtggcgac
     1081 ccacccgagc cggagtgaga ccgtgttcct cgtccccatc accgccgacc gcgagcgcat
     1141 cccacccggc gcccagctcc aggtccagcg gtcgaccgac gccggtgaca cctggacgac
     1201 gctgaccgcg gggctgccgg cgacgtcata caccgatgtc ctgcgcgacg ccctggcgct
     1261 tgacgacgcc gaccccgcgg gggtgtatgt cggcacccgc aacggcgagg tctacgcgtc
     1321 gctcgacgag ggcgactcgt tcacctgcat cgcgacgcag ctgcccgacg tgctctgcgt
     1381 tcgggcggcc accgttggct gaggcccacg tcctggtgcc gagcgcgctg cgggagtatg
     1441 tcggcggcgc cagcgcgctc gcggtggcgg tgcctgcgca ggggagcccc ctcgcggagg
     1501 tgctcggcgc cgcgctgggg ccgtggccca tggctcagcg ccgggttcgc gacgagcgtg
     1561 acgagatccg cacgcacgtc aacgtcttcg tcgacggcga ggacgtcaag cgaggggcgg
     1621 ggctggccac gacggtccgg cctgggtctc gcgtacacat cctggcgtcc gtggccggcg
     1681 gctgattgcc ggattccgcc cgagcgtgtc catgtccggg tttcgtgacg acggcgtgcc
     1741 ggagtccctg ccctcgcgtc cagcgtcgga gccaccagtc cggggcaacg gggtgagctg
     1801 aggatctgga cgagctggtg gggcgccagc gctgagacgc tggtggaacg ccgcaggtca
     1861 cctgctgggc cgagggtcag atccgggcgg aacctactct gatcgagcgc cctttcgaca
     1921 ctggcggagt cgtccgatcg gggtaatgct tcgggtatga gcaccggagc cgcagaccgc
     1981 accctgatcc tgttgcgcca tgccaaagcc gagcatgtct ggggcaagcc cgaccacgac
     2041 cgcgagctca ccacgcgcgg tcagcgcgac gcccgggccg tcggcgcgtg gctgcacgag
     2101 cacggcctca tctgcgacgt cgtcctgtgc tcgacgtcga tgcggacccg ccagacgtgg
     2161 gagggcgtcg aggccggagg tggtgctacc gaggtcatcg actacgaccc gctcatctac
     2221 ggcggcagca ccgaccggct gctgcgcgtg atccgcgacg gcgcgggcac cgccgacacg
     2281 ctcatggtcg tcgggcacgc cccctcgatc ccggacctcg tctcgatgct gtccgagggt
     2341 gaggggtcca tgcaggcgca tcaggcactc gccgcgggct tccccacgag cggggtcgcc
     2401 gtgctgagct accgaggggc atggtcggcg ctcgactacg ggacggcctc gctcgagcgc
     2461 ttccacgtct gtcgcggcta gcccccggct agcccacggc tagcccggac cctgcccgcg
     2521 cgcccgaccg gtctgcggcg cgcgggcagg gtccgggcta gccgtgggct agccgggggc
     2581 tagccgcgac agacgtggaa gcgctcgagc gaggccgtcc cgtag
//
LOCUS       DATAIX010000589         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_194556, whole genome shotgun sequence.
ACCESSION   DATAIX010000589 DATAIX010000000
VERSION     DATAIX010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194556"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..1667
                     /locus_tag="VHM65_08695"
     CDS             324..1667
                     /locus_tag="VHM65_08695"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2705824.1"
                     /translation="MLARDGITTPTPIQAATLPDSLAGRDVLGRGRTGSGKTYAFLLP
                     IVARLTSSGSDRAGRRMPRIPRAPRALILAPTRELAGQIETALKPLAASAGLTTLAVF
                     GGVGQSPQVRALRVGVDVLVACPGRLEDLIAQGECRLGAVEVTVLDEADHMADLGFLP
                     AVRRLLTATPARSQRLLFSATLDAAIDVLVKRFLTNPVTHQADSAQSPIATMEHHLLQ
                     IDAEQRLPVLVDLASASKRILVFTRTKHRAKMLARQLNAHGVPSVELHGNLAQNARTR
                     NLAAFHSGEATTLVATDIAARGIHVDDIALVVHADPPTEHKAYLHRSGRTARAGASGK
                     VVTLMQQEQVRDVRALTKAAGITATTTVVAVGHPLLEELAPGVRRFVTLLPIAESPRS
                     TPGRSASPRGDGPRSATPGGSGGRSRQGHAGAAPAGTWSASRVSEQRGGRHRRAS"
     gene            complement(1696..>2182)
                     /locus_tag="VHM65_08700"
     CDS             complement(1696..>2182)
                     /locus_tag="VHM65_08700"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX2705825.1"
                     /translation="PQRPHKRRPGDDGVMRLRAELVSHGGNTAGFVVPEGIVETLGGG
                     RRPKVVVTLGGHTWRSSITFMGGQFLLGVSMANRTAAGATTGETYDVDVTLDAAPRVV
                     AVPEDLAEALDATPGARAAWDGLSYSHQRAHVEPILAAKKPETRARRITATIDLLTTQ
                     G"
BASE COUNT          300 a          838 c          724 g          320 t
ORIGIN      
        1 cccgccagca gcgggcacgg catagcaggc cgcagcagct caatgagctg cgcgggtcaa
       61 tgagctgagc gggcggcggg gctgaactcg tgcttgacaa cgatgcgagg gattgggtgt
      121 ctgtgccgta gggttgaggc agcagcccgc cccgtcttgc cacgggctgc tggtgacgtt
      181 gtagcgatgt gcgccgcacc actcccggtt ccgactgacg agcagagtgc gcccggcagg
      241 acgtctcatc catttgttcg gagtttattc ctttgtctca caacaccttt gctgctctcg
      301 gcgtacccgc aggtctctgc gacgtgctcg cccgcgacgg catcaccact cccaccccga
      361 tccaggcggc aacgctgccc gactccctcg ccgggcgcga cgtgctcggc cgcgggcgca
      421 ccggctcggg caagacctac gcgttcctgc tgcctatcgt cgctcgcctc acgtcgtcgg
      481 gctcggaccg cgcaggccgg cgtatgccgc gcatcccccg ggcaccccgg gccctgatcc
      541 tggcaccgac ccgtgagctg gccggccaga tcgagacggc cctcaagccg ctcgcggcct
      601 cagccgggct caccacgctc gccgtcttcg gcggcgtcgg gcagtcaccg caggtgcggg
      661 cgctgcgcgt cggcgtcgac gtgctcgtcg cctgccccgg gcgcctcgag gacctcatcg
      721 cccagggcga atgccgcctc ggcgccgtcg aggtcaccgt gctcgacgag gccgaccaca
      781 tggccgacct cgggttcctc ccggccgtgc gccgcctgct cacggcgacc ccggcccgca
      841 gccagcggct cctcttctcg gccacgctcg acgccgctat cgacgtcctc gtcaagcggt
      901 tcctcaccaa cccggtgacg caccaggcgg actcggccca gtcgccgatc gccacgatgg
      961 agcaccacct cctgcagatc gacgccgagc aacgcctgcc cgtgctcgtc gacctggcga
     1021 gcgcgtccaa gcgcatcctc gtcttcaccc gcacgaagca ccgagccaag atgctggccc
     1081 gccagctcaa cgcacacggc gtgccctcgg tcgagctgca tggcaacctc gcccagaacg
     1141 cgcggacccg caacctcgcc gccttccact ccggcgaggc cacgacgctc gtcgcgaccg
     1201 acattgccgc ccgggggatc cacgtcgacg acatcgccct cgtcgtccac gccgacccgc
     1261 cgaccgagca caaggcctac ctgcaccgct cggggcgcac ggcgcgggcc ggcgcctccg
     1321 gcaaggtcgt caccctgatg cagcaggagc aggtccgcga cgtgcgggcg ctgaccaagg
     1381 cggccgggat cacggccacc acgactgtcg tcgccgtggg ccacccactg ctggaggagc
     1441 tcgccccagg cgtgcgccgg ttcgtcactc tgctcccgat cgccgagtcc ccgcggtcga
     1501 cgcccggccg cagtgcctcc cctcgggggg atggccccag gtcggcgacg ccgggtggat
     1561 ctggtgggcg cagccgccag ggccatgccg gagcggctcc ggccggcacc tggtccgcgt
     1621 cacgggtcag tgagcagcgg ggcggacgcc accgccgcgc ctcctgacaa agccctgcct
     1681 gctcatcgtg ggggctcagc cctgagtggt gagcaggtcg atcgtcgcgg tgatgcgcct
     1741 ggcccgggtc tcgggcttct tggccgcgag gatcggctcg acgtgagcgc gctggtggct
     1801 gtaggagagc ccgtcccagg cggcccgcgc acccggggtg gcatcgagcg cttccgcgag
     1861 gtcctcgggc accgccacca cgcgcggggc agcgtcaagg gtgacgtcga cgtcatacgt
     1921 ctcaccggtc gtcgcgcctg cggcagtgcg gttggccatg ctcacgccga ggaggaactg
     1981 ccctcccatg aaggtgatgc tcgagcgcca ggtatgaccc cccagcgtca ccaccacctt
     2041 gggccgacga ccaccgccga gggtctcgac gatcccctct gggacgacga aaccggcggt
     2101 gttgccgccg tggctcacca gctcggctct caggcgcatg accccatcat ccccgggtcg
     2161 acgtttgtgc ggccgctggg gg
//
LOCUS       DATAIX010000590         2673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_224197, whole genome shotgun sequence.
ACCESSION   DATAIX010000590 DATAIX010000000
VERSION     DATAIX010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224197"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1754)
                     /locus_tag="VHM65_08705"
     CDS             complement(<1..1754)
                     /locus_tag="VHM65_08705"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX2705826.1"
                     /translation="MLTPRPTTPSAPETRLARLGAVSARHPWRFVVAWLVLSAAAVTV
                     AIAGVGGGSLFSRLSTGDPSVPGEAQDAAVLLDAAEGGPTRLLQLRGVDLRDETVVSA
                     LTAATQALATVLGVTSVTSPLTVPGGPASAAAAPLLAAGSTPASTGLLTIVELDPQLP
                     AAAKDTVGAAVDAGFTRLGSAAPGAGATTGSVDELVSTITGQVEQDLRLGEGIALPVS
                     FVVMVLVFGGLVAAGMPTLGAIASILGALASLYGFSYLVDLDVSVVNVVTLLGLGLSI
                     DYGLLFVSRFREELRAIVTSPGSAGGTRASRSHRGITREQVVDATARTMARAGRTIVF
                     SALTVAIALGGLLIFEAQIMRAIGAAGVSVVVVALLAALTLVPALCALGARALMRGHR
                     DLAPEDGLFSRLARFVQKRPVPVIVLTTAVLVGLSLPAFSMRLTSSGRELLPQDNPVR
                     VFFSSLARDFPFASGPEVSVAAQGSLAEVTRWAEGSVARLPGVLSTRTEQRSPQIVAV
                     SVYVDGGAQDQASRDVVTSIRENRPAFATWVAGPAARLADFTTSMAARAPFAGAVVAL
                     GTLALLFLLTGSLVIPLKA"
     gene            1800..>2673
                     /locus_tag="VHM65_08710"
     CDS             1800..>2673
                     /locus_tag="VHM65_08710"
                     /inference="COORDINATES: protein motif:HMM:NF017321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2705827.1"
                     /translation="MAAADLFGAVEPDDPALVPPLAVRMRPRTLDEVEGQGAVLGPGS
                     PLRRLIEGAAGAAGPLSAIIWGPPGTGKTTLAHLVATAAGRRFVELSAVTAGVRDVRA
                     VIDAATRERDLYGRHTVLFLDEIHRFSKAQQDALLPGVENRIVILVAATTENPSFSVI
                     APLLSRSVLVSLGPLSDEEIGRVLDRALADPRGLAGQLVLDADARDHLVRTAGGDARR
                     ALTALEAAAGVAGDDGVARVSLGQVERAVAYAVVRYDRAGDQHYDVASALIKSMRGSD
                     VDAALHYLARMLEAG"
BASE COUNT          386 a          939 c         1036 g          312 t
ORIGIN      
        1 gccttgaggg gtatgacgag cgagccggtg agcaggaaga gcagcgcgag cgtgccgagg
       61 gcgacgacgg ctccggcgaa cggggcccgg gcggccatcg aggtggtgaa gtcggcgagg
      121 cgggccgcag gcccggccac ccacgtcgcg aaggcgggac ggttctcgcg gatcgaggtc
      181 acgacgtcgc gcgacgcctg gtcctgggcc ccgccgtcga catagacgga caccgcgacg
      241 atctgcggtg agcgctgctc ggtccgcgtc gacaggactc cggggagccg ggcgacgctg
      301 ccttcggccc acctcgtcac ctcggccagg gacccctggg ctgcgaccga gacctcgggg
      361 ccgctggcga aggggaagtc gcgagcgagc gaggagaaga agacccggac ggggttgtcc
      421 tgggggagca gctcgcggcc cgaggaggtg agccgcatcg agaaggccgg cagggaaagc
      481 ccgacgagga ccgccgtcgt cagcacgatg accgggaccg gccgcttctg gacgaaccgg
      541 gccagccgcg agaagagccc gtcctcgggc gcgaggtcgc ggtggccccg catgagcgct
      601 ctggcgccga gcgcgcagag cgcggggacc agggtcaggg cggcaagcag ggcaacgacg
      661 acgacggaca cccccgcggc accgatcgcg cgcatgatct gggcctcgaa gatgagcagg
      721 ccgccgagcg cgatggccac ggtcagcgcg ctgaacacga tggtccgccc ggcgcgcgcc
      781 atggtgcgtg ccgtggcgtc gacgacctgt tctcgggtga tgccgcggtg ggaccggctg
      841 gccctggtcc cccccgccga cccgggcgac gtgacgatcg cccgcagctc ctcgcggaac
      901 cggctcacga agagcaggcc gtagtcgatg gacagcccga gcccgagcag ggtgaccacg
      961 ttgacgacgc tgacatcgag gtcgacgagg taggagaagc cgtagagcga ggccagcgcg
     1021 ccgaggatcg acgcgatggc cccgagagtc ggcattccgg cggcgacgag gccgccgaag
     1081 acgagcacca tgacgacgaa gctgaccggc agggcgatgc cctccccgag gcgcaggtcc
     1141 tgctcgacct gaccggtgat cgtcgagacg agctcgtcca ccgagcccgt cgtcgcgccg
     1201 gcccctggcg cggccgagcc gaggcgggtg aaccctgcgt cgacggcggc gccgaccgtg
     1261 tccttcgcgg ctgcgggcag ctgtgggtcg agctcgacga tggtgagcag gcccgtcgag
     1321 gcgggggtgc tgcccgctgc caggagaggg gcggccgccg ccgaggccgg cccacccggg
     1381 acggtgagcg gcgaggtgac cgacgtgacc cctagcacgg tcgccagggc ctgggtcgcg
     1441 gccgtcaggg ccgacacgac ggtctcgtcg cgcaggtcga ccccgcgcag ctggagcagc
     1501 cgcgtcggtc cgccctcggc ggcgtcgagc agcaccgcgg cgtcctgggc ctcgcccggg
     1561 acgctcgggt cgccggtgct gagccggctg aacagcgacc ccccgccgac cccggcgatc
     1621 gccacggtga ccgcggccgc cgacaggacc agccaggcca cgacgaaacg ccatgggtgg
     1681 cgggcactca cggcgccgag gcgggcgaga cgggtctcgg gagcggacgg cgtcgtgggc
     1741 cgcggggtca gcacggggat agcctgtcat gcacgccgac gttgtgagga ggtgcgagta
     1801 tggcggccgc ggacctgttc ggtgcggtgg aaccggacga cccggcgctc gtcccaccgt
     1861 tggcggtgcg gatgcgtcca cgcaccctcg acgaggtcga aggacagggt gccgtgctgg
     1921 gcccgggcag ccccctgcgc cggctcatcg agggcgcggc tggtgccgcg ggcccgctgt
     1981 ccgcgatcat ctgggggccg ccggggaccg gcaagaccac cctggcccat ctcgtcgcga
     2041 cggcagccgg tcgccgcttc gtcgagctgt ccgccgtgac ggccggggtg cgcgacgtcc
     2101 gggctgtcat cgacgcggcg acccgcgagc gggatctcta cgggcgccat accgtgctgt
     2161 tcctcgacga gatccaccgc ttctccaagg cacagcagga cgcgctgctg ccgggggtcg
     2221 agaaccgcat cgtcatcctc gtcgccgcca cgaccgagaa cccgtcgttc agcgtcatcg
     2281 cgccgctgct ctcccgatcg gtgctcgtct cgctcgggcc gctctccgac gaggagatcg
     2341 ggcgcgtgct cgaccgggcg ctcgccgacc cgcggggcct ggcgggccag ctcgtcctcg
     2401 acgccgacgc ccgcgaccat ctcgtgcgca ccgccggtgg tgatgcccgg cgcgccctca
     2461 ccgccctcga ggcggccgcg ggcgtggccg gtgacgacgg cgtcgcgcgg gtctcgctgg
     2521 ggcaggtcga gcgcgccgtc gcgtatgccg tggtccgcta cgaccgcgcc ggggaccagc
     2581 actacgacgt cgcgagcgcc ctcatcaagt cgatgcgggg cagcgatgtc gacgcggccc
     2641 tgcattacct cgcgcggatg ctcgaggcag ggg
//
LOCUS       DATAIX010000591         3734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_134753, whole genome shotgun sequence.
ACCESSION   DATAIX010000591 DATAIX010000000
VERSION     DATAIX010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134753"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..897
                     /locus_tag="VHM65_08715"
     CDS             121..897
                     /locus_tag="VHM65_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX2705828.1"
                     /translation="MTDFTSENLTGARFEDVYLTGARFHDVDLSNARFRLVDLSGVMI
                     RGAALVNVDISGEIENLRVNGVDVVPLVEAELSRRYPDRVKMRPADAGGFAEAWDILE
                     RLWARTVERARAMAPGLLHERVEEEWSFIETLRHLVFATDAWVSRAILGKPSPWDPLD
                     LPHDEMPEEPSVPRERGARPSLEEVLALRADRMATVRQVIAALTDERLAGMTEPVLEP
                     GYPEPRSFAIRRCLQAVLNEEWEHRLYAERDLDVLESRSS"
     gene            complement(966..1352)
                     /locus_tag="VHM65_08720"
     CDS             complement(966..1352)
                     /locus_tag="VHM65_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705829.1"
                     /translation="MVIDPENPYAGQGPVLLDLDDDHGALIVHTPPDLVGAEIELHAL
                     DPMVEHTHTGGHPHTHVAEGSPHGHHAPHVAVHPRPMPAGAEMPSAVFVSLAPGRYAC
                     SLIVDHVRRLVATVKPGEVTFADWPA"
     gene            complement(1419..2798)
                     /locus_tag="VHM65_08725"
     CDS             complement(1419..2798)
                     /locus_tag="VHM65_08725"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEX2705830.1"
                     /translation="MSSHREAPEISKDPAADNTDVYAFVSPDKPGTVTLISNFIPFQN
                     PQGGPNFYEFGDEVLYEIHVCNSGDAKPDVTYQFRFQTRIRNDKTFLYNTGQIVNITD
                     PTWNRPQYYSVTRVARGKAPQVLAEKLTCPPVNVGVRSTPHYPKLAAQAIHRIKGGRT
                     VFAGQRAEGFFVDLGSIFDLGALRPFNMAHLIPIVAAAGVNGTQGLNVHTIAIQVPIT
                     DLTPRGVRPTHVNDPEATIGIYSSASRRSATIRSESSGNPKELGPFVQVSRLGNPLFN
                     EVIVPMSRKDTWNVRPPYRDADFAALVAQPELARLLPALYPGVFPNLAAYTAPRADLS
                     AILLTGIPQGVVPGFQNYTGPTPADLLRLNVAVPPSTTPNPLGVVAGDPAGFPNGRRV
                     FDDVVTVELRAVAGLTIPLVDPSFTPDGATSVIADGTSNTNAPYRRTFPYLGEPAGGY
                     QSTPGTAVV"
     gene            complement(2800..3087)
                     /locus_tag="VHM65_08730"
     CDS             complement(2800..3087)
                     /locus_tag="VHM65_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705831.1"
                     /translation="MSDHTRRGFLVIADTGAAAVGAAVATGSAMAGVNERMDAGASTE
                     SGEASARGPVVAYIEDLDRGEIVLISGESRTVLTDRALVSGLARLAAKGGA"
     gene            3180..3374
                     /locus_tag="VHM65_08735"
     CDS             3180..3374
                     /locus_tag="VHM65_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705832.1"
                     /translation="MDHRVASSGGSPEKLQVSERLAGLLRRSAGGDQPAFADLYDAVL
                     PGVYGVVCYALCAIAPRPRR"
     gene            3344..>3734
                     /locus_tag="VHM65_08740"
     CDS             3344..>3734
                     /locus_tag="VHM65_08740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2705833.1"
                     /translation="MRDRAAAEEVTQEAFLEIWRTASRFDQHRGTALSWVLMIAHRKA
                     VDRVRSAESAHRRDQAARSVAHDIPYDVTADSAYASIEAERVRGALASLSAAQRGALE
                     LAFLGGYSHSEVAAMLGLPIGTAKTRIR"
BASE COUNT          540 a         1207 c         1402 g          585 t
ORIGIN      
        1 catcacacct gcccatcgcg ctggcctgag cccggccggt gacactgacc gagcacggca
       61 gggggacctc gtgaggtccg gcctctcgac gggccgcggc agtcgcggga ggctggccgg
      121 gtgactgact tcaccagcga gaacctgacc ggcgcccggt tcgaggacgt ctacctgacc
      181 ggagcccggt tccacgacgt cgacctgagc aacgcgcgct tccgcctggt cgatctgagc
      241 ggggtgatga tccggggcgc ggccctggtg aacgtagaca tcagcggcga gatcgagaac
      301 ctgcgggtca acggcgtcga cgtcgtgccg ctggtggagg ccgagctcag ccgccgctac
      361 ccggaccggg tgaagatgcg gccggccgat gccggcgggt tcgccgaggc atgggacatt
      421 ctcgagcggc tctgggcgcg gaccgtcgag cgggcccgcg cgatggcccc ggggctgctg
      481 cacgagcggg tcgaggaaga gtggtccttc atcgagaccc tgcgccatct cgtcttcgcc
      541 accgacgcct gggtcagccg cgcgatcctg gggaagccgt cgccgtggga cccgctggac
      601 ctgccccacg acgagatgcc cgaggaaccc agcgtcccgc gcgagcgcgg cgcgcgcccg
      661 tcgctggagg aggtgctcgc actccgcgcg gaccgtatgg cgaccgtgcg tcaggtgatc
      721 gccgctctta ccgacgagag gctggccggg atgaccgaac cagtcttgga gccgggctac
      781 ccggagccgc ggagcttcgc catacgccgc tgcctccagg ccgtcctcaa cgaagagtgg
      841 gagcaccggc tgtacgccga acgggacctc gacgtgctcg agtcccgctc gtcctgaccc
      901 gcatgagctg cgaggacgcg tcgacggtcg catcagcggc ccctgactgc cggtcagcgg
      961 gcacctcagg caggccagtc ggcgaacgtc acctcaccgg gcttgacggt ggccacgagc
     1021 cgacggacgt ggtcgacgat gagcgagcag gcgtatcggc caggcgcgag ggacacgaag
     1081 acggccgagg gcatctcggc tccggccggc atcggccgcg ggtggacggc gacgtgcggc
     1141 gcgtggtgcc catgaggcga cccttcagcc acgtgggtgt gggggtgtcc tccggtgtgg
     1201 gtgtgctcga ccatcgggtc gagggcgtgc agctcgatct cggcgccgac gaggtcgggg
     1261 ggtgtgtgca cgatgagcgc cccatggtcg tcgtcgaggt cgagcaggac gggaccttgc
     1321 ccggcatacg ggttctcggg gtcgatcacc atgttcgcct ccttgggtgc tccccggccg
     1381 cctacccccc ccatcggcga ccggggagcg ttgatgggtc agaccacggc ggtgcccggc
     1441 gtgctctggt agccacccgc cggttcgccg aggtagggga aggtgcgccg gtagggggcg
     1501 ttcgtgttgg acgtgccgtc cgcgatcacc gacgtcgccc cgtcgggggt gaacgacggg
     1561 tcgacgagcg gaatggtcag cccggcaacg gctcgcagct cgacggtgac gacgtcgtcg
     1621 aagaccctcc ggccgttggg gaatccggcc gggtcgccgg cgacgacccc gagcgggttg
     1681 ggggtggtcg agggcggcac ggcgacgttg agccgcagca ggtcggccgg tgtgggtccg
     1741 gtgtagttct ggaaccccgg gacgacccct tgcggtatgc cggtgagcag gatcgcggac
     1801 aggtccgccc tcggcgcggt gtaggcggcg aggttcggga agaccccggg gtagagcgcc
     1861 ggtaacagcc gcgcgagctc gggctgggcc acgagagcag cgaagtcggc gtcccggtag
     1921 ggcggtcgga cgttccacgt gtccttgcgg ctcatcggca cgatgacctc gttgaagagc
     1981 gggttgccga gccgtgagac ctggacgaac gggcccagct ccttggggtt ccccgaggac
     2041 tccgagcgga tcgtggccga gcgccggctg gccgaggagt agatcccgat cgtggcttcc
     2101 gggtcgttga cgtgggtcgg ccgcaccccg cgtggggtca ggtcggtgat cggcacctgg
     2161 atcgcgatgg tgtgcacgtt gagcccctgg gtgccgttga cgccggccgc cgcgacgatg
     2221 gggatcaggt gggccatgtt gaacggccgc aacgccccga ggtcgaagat gctgcccagg
     2281 tcgacgaaga agccctcggc ccgctggccc gcaaagacgg tccgcccgcc cttgatccgg
     2341 tggatcgcct gggcggccag cttcgggtag tgcggggtgc tgcggacgcc gacgttgacc
     2401 ggggggcagg tcagcttctc ggcgagcacc tggggtgcct tgccccgggc gactcgcgtg
     2461 accgagtagt actgaggccg gttccaggtc gggtcggtga tgttcacgat ctggccggtg
     2521 ttgtagagga acgtcttgtc gtttcggatg cgcgtctgga accggaactg gtaggtgacg
     2581 tcgggtttgg cgtcgccgga gttgcagaca tggatctcgt agaggacctc gtcgccgaac
     2641 tcgtagaagt tcggcccacc ctgcgggttc tggaacggga tgaagttcga gatcagcgtc
     2701 acggtgccgg gcttgtccgg actgacgaag gcatagacgt cggtgttgtc ggcggccggg
     2761 tccttggaga tctcaggagc ttcgcgatgc gacgacatgt caggcaccac ccttcgcggc
     2821 gagccgggcc aggcccgaga cgagcgcccg gtcggtgagg accgtcctcg actcgcccga
     2881 gatcagcacg atctcgccgc ggtcaaggtc ctcgatgtag gcaaccaccg ggccacgggc
     2941 actcgcctcg cccgactcgg tcgacgctcc agcgtccatg cgttcgttga cgccggccat
     3001 cgcggaccca gtcgcgacgg ccgcgcccac ggctgcggcg cccgtgtcgg caatgacgag
     3061 gaaccctcgt cgagtgtgat cagacatgac aactccctcg cggagatgga caacgatggc
     3121 ccacagttcg cgtcagtccc cgggtcggat tggcccgacc cggtgtgaca atggcggaca
     3181 tggaccatcg ggttgcctcc tccggcgggt cgccggagaa gctgcaggtg tccgagcggc
     3241 tcgctggcct gctcaggcgc tcggcgggcg gcgaccagcc ggccttcgcc gatctgtacg
     3301 acgcggtcct gccgggggtg tatggcgtgg tgtgttacgc cttgtgcgcg atcgcgccgc
     3361 ggccgaggag gtgacccagg aggcgttcct ggagatctgg cggacggcga gtcgcttcga
     3421 ccagcaccgg ggcaccgcct tgagctgggt gctgatgatc gcgcaccgca aggccgtcga
     3481 ccgggtgcgc tcggccgagt cggctcatcg ccgtgaccag gctgctcgca gcgtcgcgca
     3541 cgacattccg tatgacgtga cggccgactc cgcctacgcc tcgatcgaag ccgaacgggt
     3601 ccgtggggcg ttggcgtcct tgagcgcggc ccagcgcggg gcgctcgagc tggccttcct
     3661 cggtgggtac tcccactccg aggtggccgc catgctcggc ctgccgatcg gaaccgccaa
     3721 gacacggatt cgtg
//
LOCUS       DATAIX010000592         3215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_224328, whole genome shotgun sequence.
ACCESSION   DATAIX010000592 DATAIX010000000
VERSION     DATAIX010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224328"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1651)
                     /locus_tag="VHM65_08745"
     CDS             complement(179..1651)
                     /locus_tag="VHM65_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX2705834.1"
                     /translation="MTTRPQTSGLARHLGRVGTELETLQTGRYGDPLARRSVWGPALD
                     VPLPREGAGADAVVEEIATTLIPNGARFGEAGFSGWIVSTPGTVATVAQAAATVASSQ
                     RYTLTAFNLVEEVSLRWLAELCGLDPGRMLGVYSSGGSVANLVALGAARQWAFEQRGV
                     DVAADGTGGRLTAVYASAETHHTVHRAAAVLGLGRSSVRAVPTDSAQRMDVDALAAML
                     AADQAAGVIPVAVVANAGTTNTGAIDPIRAAGEAAQAAGAWFHVDGAYGLLGSLDERV
                     AGLYDGLELADSAIVDPHKWLNAPTGTAATFVRDRAILYRAFTQEPAAYLESVFSPAD
                     DAQSSLDSVGIPYSEFGVELSAPARGTAVWAVIRELGRSGVAARVREDRDLASRLAEV
                     ARRHPRLEVLAEPVLSICCVRYTAPGLADHDRVNEAILRRLLRDTPYLPSSTRVGGAF
                     AIRPCYINPRTTPEHVDGLAAAIVEIGDEIIGDEITAEFA"
     gene            1755..3080
                     /locus_tag="VHM65_08750"
     CDS             1755..3080
                     /locus_tag="VHM65_08750"
                     /inference="COORDINATES: protein motif:HMM:NF002829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX2705835.1"
                     /translation="MPYSAADRERWCAEDPAHKRADRDDFARDRARVVHSAGLRRLSA
                     KTQVVQPGSDDFVRNRLTHSLEVAQIGREFGAALGCDADVVDTACLAHDLGHPPFGHN
                     GETALNALTDGIGGFEGNAQTLRLVTRLEAKRSHPDGRPAGLNLTRASLDAVTKYPWA
                     RGHGPAPDKFGVYPDDRPVFEWYRSTVPEPRTRCIEAQVMDFADDVAYSVHDIEDGVA
                     SGRLDVRVLHDDAEMAPALEVARTLYAPESDAEALGAARARLLATGTVPASYTGSRGD
                     LAALKDMTSRLIGRFVHAVELATRERHGRGRLTRYGADLAIPGEVRAEIAVLKGTAAH
                     FVMLVDERLAVMALQRDAVADLVAAYRADPLGRLDPDLRADFAAAADDVARLRVVVDQ
                     VASLTDARAWSLHAQWSTGQWSTPAAPTTSAPTTSAPTTSAPTTSAPTT"
BASE COUNT          472 a         1210 c         1130 g          403 t
ORIGIN      
        1 gtcatggatc ggcacatccc gcaaggcatt gaaggcatat atgagaacgg aattcgtcga
       61 gggtggtcgg tcgggccagg gcgcgacgat ctcgctcgct cgcccgccag tcgggccgcg
      121 ctcccagcgc cagtcgggcc gggctcccag cgccggccgc gcatgcgccc cggagccgtc
      181 aggcgaactc cgctgtgatc tcgtcaccga tgatctcgtc accgatctcg acgatcgcgg
      241 ccgcgagccc gtccacatgc tcgggagtgg ttcgcgggtt gatgtagcac ggccggatcg
      301 cgaaggcacc cccgacgcgc gtcgagctgg gcaggtaggg ggtatcccgg agcagccggc
      361 gcaggatcgc ctcgttgacg cggtcatggt cggcgagtcc aggcgcggtg tagcgcacgc
      421 agcagatcga cagcaccggc tccgcgagga cctcgaggcg cgggtgtcgc cgggccacct
      481 cggcgagtcg cgaggcgagg tcgcggtcct cccgcacccg cgcagcaaca cccgagcggc
      541 ccagctcgcg gatcaccgcc cacacggccg ttccccgcgc gggtgccgac agctcgacac
      601 cgaactccga gtaggggatc cccacactgt cgagcgagct ctgcgcgtca tcagccggcg
      661 agaagaccga ctcgagatag gccgccggct cctgggtgaa ggcgcggtac aggatggccc
      721 ggtcgcgcac gaacgtcgcg gccgtccccg tcggggcgtt gagccacttg tgcgggtcca
      781 cgatggccga gtccgcgagc tccagaccgt catacaaacc agccactcgc tcgtcgaggg
      841 accccagcag cccgtacgcc ccgtcgacgt ggaaccacgc cccggctgcc tgcgccgcct
      901 ccccggccgc ccggatcggg tcgatcgccc cggtgttcgt cgtccctgcg ttggccacca
      961 cggcaacggg tatgacgccc gccgcctggt ccgcggcgag catggccgcc agcgcgtcca
     1021 catccatccg ctgggccgag tcggtcggga cggcccggac gctggaacgc ccgagcccga
     1081 gaacggccgc ggcgcgatgc accgtgtggt gggtctcggc cgaggcatag accgccgtca
     1141 ggcggccgcc ggtgccgtcg gcggcgacat cgacgccgcg ctgctcgaag gcccactgcc
     1201 gggccgcgcc gagggcgacg aggttggcga ccgaaccacc gctggagtag accccgagca
     1261 tccggccggg gtcgaggcca cacagctcgg ccagccagcg cagcgagacc tcctcgacga
     1321 ggttgaacgc cgtgagggtg tagcgctgcg acgacgccac ggtggccgcg gcctgcgcga
     1381 cggtggccac ggtgcccggt gtggagacga tccagccgga gaagcccgcc tcgccgaagc
     1441 gtgcgccgtt ggggatgagc gtcgtggcga tctcctcgac cacagcatcg gccccggcgc
     1501 cctcacgtgg cagcggcacg tccagcgccg ggccccacac cgagcggcgg gcgagcgggt
     1561 cgccataccg gccggtctgg agggtctcca gctcggtacc gacccggccc agatgcctgg
     1621 ccaggccgga ggtctgcggt cgtgtcgtca tcgtgtcacc atcccgacgc caccctcggg
     1681 cgtgtcgtca gcagtatgcc tccccggagc cccggccggg aggtgggccg tccctgcacg
     1741 gctagagtcg gacggtgccc tacagcgctg cggaccgcga gcggtggtgt gccgaggacc
     1801 ccgcccacaa gcgtgccgac cgggacgact tcgcgcggga ccgggcccgg gtcgtccact
     1861 cggccgggct gcgcaggctg tccgcgaaga cccaggtggt ccagcccggc agcgacgact
     1921 tcgtccgcaa ccggctcacc cactccctcg aagtcgccca gatcggaagg gagttcggtg
     1981 ccgcgctcgg ctgcgacgcc gatgtcgtgg acaccgcgtg cctcgcccac gacctcgggc
     2041 acccaccgtt cgggcacaac ggcgagacgg cgctcaacgc cctgaccgac ggcatcggcg
     2101 ggttcgaggg caacgcccag accctgcggc tcgtgacccg gctcgaggcc aagcgcagcc
     2161 acccggacgg gcgccccgcc gggctcaacc tcacccgggc cagcctcgac gcggtgacga
     2221 agtacccctg ggcgcgcggg cacggtccgg caccggacaa gttcggcgtc taccccgacg
     2281 acaggcccgt cttcgagtgg taccgcagca cggtgcccga gccacgcacc cggtgcatcg
     2341 aggcccaggt catggacttc gccgacgacg tggcctactc ggtccacgac atcgaggacg
     2401 gggtcgcctc cggacgcctc gacgtgcggg tcctgcatga cgacgcggag atggcgcccg
     2461 cgctcgaggt cgcccgcacg ctctatgccc ccgagagcga cgcagaggcg ctcggcgccg
     2521 cccgcgcgcg gcttctggcc acgggaacgg tgcccgcgtc atacaccggg tcgcgcgggg
     2581 acctggccgc gctcaaggac atgacgagcc ggctcatcgg ccgcttcgtg cacgccgtcg
     2641 agctcgccac ccgcgaacgg cacggccgcg gacgcctgac ccggtatggc gcggacctcg
     2701 ccatcccggg cgaggtgagg gccgagatcg ccgtgctcaa ggggacggcc gcccacttcg
     2761 tcatgctcgt cgacgagcgc ctcgcggtga tggcgctcca gcgggacgcg gtggccgacc
     2821 tcgtcgcggc ctaccgggct gacccgctgg gccgcctcga cccggatctg cgcgccgact
     2881 tcgccgccgc cgccgacgac gtcgcgcggc tgcgcgtcgt cgtcgaccag gttgcctcgc
     2941 tcaccgatgc gcgggcgtgg agcctgcacg cacagtggtc gacaggacag tggtcgacgc
     3001 cggccgcacc gacgacctcc gcaccgacga cctccgcacc gacgacctcc gcaccgacga
     3061 cctccgcacc gacgacctag actccccctg tcggaaggga gcgcgcgtgg ccgggctgat
     3121 caaggccgag gacatcgctg ccatcaagga gcggtcctcg atcgaggacg tcgtgcgcga
     3181 gcatgtcacc cttcgctcgg ccggccctgg ctcgc
//
LOCUS       DATAIX010000593         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_224332, whole genome shotgun sequence.
ACCESSION   DATAIX010000593 DATAIX010000000
VERSION     DATAIX010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224332"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..459)
                     /gene="rpmE"
                     /locus_tag="VHM65_08755"
     CDS             complement(244..459)
                     /gene="rpmE"
                     /locus_tag="VHM65_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX2705836.1"
                     /translation="MRQDIHPKYGETQVTCTCGNSFTTRSTAKNGVIHADVCSQCHPF
                     YTGKQKILDTGGRVARFQARYGTKVAK"
     gene            593..1438
                     /locus_tag="VHM65_08760"
     CDS             593..1438
                     /locus_tag="VHM65_08760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2705837.1"
                     /translation="MRFGYVVPFTTERQFAELATLGEASGWDAVFAWEAVWGQDAWAT
                     LGAASMVTSRIRLGTLLTPASRIRPWDLASRVGSVDRLSSGRVTLGVGLGALNGNWLA
                     FEPDEGRAARARKLDECLAVYAGLMGGQPFAYQGGHYSAREVPELIPPLPVQRPHPPV
                     WVVGALAPGRTRQRSLERAARWQGIFPAVLGAAGSSGLTLDALEDIATRLRALRAQLD
                     LPWEGYDVVVEGDSHGGFGTVRGPASTWAAAGATWWVESWWDLPDSDEGRAELRRRIE
                     LGPVT"
     gene            1566..2048
                     /locus_tag="VHM65_08765"
     CDS             1566..2048
                     /locus_tag="VHM65_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2705838.1"
                     /translation="MVRPALALDAPVLVTLRELMFEAMGTPAALLAGSRWRPAALAWF
                     EAASADPDIRVVVAQIGGDVVSCGVGEVTRLIPGPSTPNGAVGLVSNIATVPAARGQG
                     LASACLDALLAWFDAETAVTRVDLFATPGGAVIYERRGFTEGTFPAMRRPSPVRRPPY
                     "
BASE COUNT          240 a          742 c          768 g          342 t
ORIGIN      
        1 atggcggcca cggtcgagcc gagcgcggca tagcgcttgt tgagccggcg gaccaggtcc
       61 tggtcggtgt ggaccgaagg gtcggcgagg cgacgttcga gctcggcgtg ctcgtcgagg
      121 aaggtcgtgg cgctctcgag catcgaggcc ccgtggtcgc tcatctcatc ccgtcggtcg
      181 tggtggcacc aacacgaacg ccggcccgcg tgacgggtgt cacgggaccg gcgtacggga
      241 cggctacttg gcgaccttgg tgccgtagcg cgcctggaag cgggcgaccc ggccaccggt
      301 gtcgaggatc ttctgcttac ccgtgtagaa cgggtggcac tgagagcaca cgtcggcgtg
      361 gatgacgccg ttcttcgcgg tgctgcgggt ggtgaacgag ttgccgcagg tgcacgtcac
      421 ctgggtctcg ccatacttcg ggtggatgtc ttgacgcacg gtcgctcctt gggtgacgtg
      481 gtccgggtcg gctggtgccg tgaaccgggc cagtgagcaa gtgtgccaga ggttgcggca
      541 cgccacctaa acggcgggtg tcctgtcggc attcccgcgt atcgtcccgg gcatgagatt
      601 cgggtatgtc gttccgttca cgaccgagcg ccagttcgcc gagctggcca ccctcggcga
      661 ggccagcggc tgggacgcgg tcttcgcgtg ggaggcggtc tggggccagg acgcctgggc
      721 cacccttggc gcggcctcga tggtgacctc ccgcatccgg ctggggacgc tgctcacgcc
      781 cgcctcacgg atccggccgt gggaccttgc ctcgcgggtc gggtcggtcg accggctcag
      841 ctcgggccgg gtcaccctcg gggtcggcct cggagcgctg aacggcaact ggcttgcctt
      901 cgagcccgac gagggccgcg cggcccgggc ccgcaagctc gacgagtgcc tcgcggtgta
      961 cgccgggctc atgggcggac aacccttcgc ctaccagggg gggcactaca gcgcacggga
     1021 ggtgcccgag ctcatccctc ctctgccggt gcagcgaccc cacccgccgg tctgggtcgt
     1081 cggagcgctc gcgcctgggc gcactcggca gcgctcgctg gagcgggccg cccggtggca
     1141 gggcatcttc ccggccgtcc tcggggccgc gggcagctcc ggtctcaccc tggacgcact
     1201 cgaggacatc gcgacgaggc tgcgcgcact gcgcgcacag ctcgacctgc cgtgggaggg
     1261 ctacgacgtg gtcgtcgaag gtgactccca tggcggcttc gggaccgtgc gcgggccggc
     1321 ctcgacgtgg gcagccgccg gagcgacctg gtgggtcgag agctggtggg acctgcccga
     1381 ctccgacgag ggccgtgccg agctgcgccg ccgcatcgag ctgggtccgg tgacgtgagc
     1441 gcccgggtga ggttcacccc ggcgagcgct cggcgcgtcc gatcccgctc tccgtcgtcc
     1501 tgccccggct caccggggcc catgtcgtcc tgcctgtttt gcccgtcctg cgcgccgtga
     1561 cagccatggt tcggcctgcc ctcgccctcg atgcccctgt tctcgtgacg ctgcgcgagc
     1621 tgatgttcga ggcgatgggc accccagcgg cactcctggc tggttcgcgg tggcggcccg
     1681 ccgcgctggc ctggttcgag gccgcctcgg ccgaccctga catccgggtc gtcgtcgcgc
     1741 agatcggcgg cgacgtcgtg tcctgcggcg tcggcgaggt cacgcgcctc atcccgggcc
     1801 cgtcgacacc caacggcgcg gtcgggctcg tctccaacat cgcaacggtc ccggccgcgc
     1861 gcggccaggg gctggcctcg gcctgcctcg acgcgctgct ggcctggttc gacgcggaga
     1921 cggcggtcac tcgggtcgac ctgttcgcca cgcctggcgg ggcggtcatc tacgagcggc
     1981 gcggcttcac cgagggcacc ttccctgcga tgcgccgccc cagccccgtc cgtcgcccgc
     2041 cgtactagct cgccagcgcg gaaaccctcc cctggggcga cttcccgtgc cc
//
LOCUS       DATAIX010000594         3096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_284337, whole genome shotgun sequence.
ACCESSION   DATAIX010000594 DATAIX010000000
VERSION     DATAIX010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284337"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VHM65_08770"
     CDS             complement(<1..464)
                     /locus_tag="VHM65_08770"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX2705839.1"
                     /translation="MTRSDLFRTALAERVLVADGAMGTMLQAADPSLEDFQGLEGCNE
                     ILNVSRPDIVRAVHDAYFAAGVDMVETNTFGANLANLGEYDIADRVFELSRAGAQIAA
                     EAAARWSTPERPRFAVGSVGPGTKLPSLGHIEFAELRDAYVPQMEGLLEGGA"
     gene            588..1452
                     /locus_tag="VHM65_08775"
                     /pseudo
     CDS             588..1452
                     /locus_tag="VHM65_08775"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
     gene            complement(1449..2702)
                     /gene="mshC"
                     /locus_tag="VHM65_08780"
     CDS             complement(1449..2702)
                     /gene="mshC"
                     /locus_tag="VHM65_08780"
                     /EC_number="6.3.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--1-D-myo-inosityl
                     2-amino-2-deoxy-alpha-D-glucopyranoside ligase"
                     /protein_id="HEX2705840.1"
                     /translation="MISWPSPSIPAVPGTGPVLRLYDTSARAVRPVEVTGEAGLYVCG
                     ITPYDATHLGHAATYVTFDLVGRALRDAGHQVRYVQNVTDVDDPLLARAVRDGHDWRD
                     LASRETALFREDMTALGVIAPDHYIGVVESVPRIVDVVAALVAGGAAYGLAVPGDEGT
                     GHDVYLDLTAAPAFGEVGHLSRDDMLELFAQRGGDPGRSGKRDPLDPLLWRAEREGEP
                     AWEGGSLGFGRPGWHIECTTIALDYLGMGFTIQGGGSDLVFPHHEMSAVQAEAVTGER
                     TFASSYVHQAMVGLDGEKMSKSKGNLVLVSELRRQGVDPMAIRLALLGRHYRTEWSWT
                     DPLLVEAQHRLDTWRAALSVNAGPAAEAMVAMVRERLGDDLDGAGALAAVDAWAASCL
                     TDGGDDPGAPGLAARTVDALLGVRL"
     gene            complement(2755..>3096)
                     /locus_tag="VHM65_08785"
     CDS             complement(2755..>3096)
                     /locus_tag="VHM65_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705841.1"
                     /translation="VTNNSDRKGSHVVRDAAGRLWGFDHGVSFHVEPKLRTVLWGWAG
                     QPLTPADLERLGRLDEALADAGSRLHRDLLVALPREDVGALTGRVRRLLATGVHPAPT
                     PGWPSIPWPAL"
BASE COUNT          447 a         1122 c         1111 g          416 t
ORIGIN      
        1 tcggcgccgc cctcgagcag cccctccatc tgggggacgt aggcgtcgcg cagctcggcg
       61 aactcgatgt ggccgagcga ggggagcttg gtgcccgggc cgaccgagcc caccgcgaac
      121 cgggggcgtt ccggggtcga ccagcgggcg gcggcctcgg cggcgatctg cgcgccggcc
      181 cgggagagct cgaagacgcg gtcggcgatg tcatactcgc cgaggttggc caggttggcg
      241 ccgaacgtgt tggtctcgac catgtcgaca ccggcggcaa agtaggcgtc gtggaccgcg
      301 cggacgatgt cgggccggct cacgttgagg atctcgttgc agccctcgag gccctggaag
      361 tcctcgaggc tcggatcggc cgcctggagc atcgtgccca tcgccccgtc tgcgaccagg
      421 acgcgctcgg cgagcgcggt acggaacagg tcagagcgcg tcacgagcag ctttcgggta
      481 cgtcgaggtc ggtggcctgc ccgcgagtct agtcgtcgag gtcgaccccc gatcctcggg
      541 ttgcataggc tggacgtacc cgacctggct ttggaggctg gagatctgtg atcgagctcg
      601 tcgacgtcct tgacctgcgc gaccccatcc tcatcgcggc cttcgagggc tggaacgacg
      661 cgggtgaggc ggctacgtcg acgatcgccc acctcgccca cgtctggaat gccgagctca
      721 tcgcgcaggt cgaccccgag gactactacg acttccaggt caaccgcccg cgggtgtcct
      781 ccgacggggg ggctgcgccg gatcgactgg ccgacgaccc gcgtgctcgc ctgcacagac
      841 accctgatcg gcccggacat cctgctcgtc gcgggcatcg agccgtcgac gcgctggcgc
      901 accttcaccc tcgaggtgct cgagtacgcc caggcgctcg gggtcaccca ggtcatcgtc
      961 ctcggcgcgc tgctcgctga cgtcccgcac acccggcccc ttcccgtgac gagcacctcc
     1021 gatgacccgg cggtccagcg tgggcacgac atcgaggaga gcacctacga ggggccgacc
     1081 ggcatcgtcg gcgtcctggc cgaggccgcg accaaggcag ggatcccggc tctgtcctgc
     1141 tgggtcgccg tgccgcacta cgcgggcgga gccccctccc cgaaggctgc gctcgggctg
     1201 gtcagccgac tcgagtccca cctcaaggtc acgatccccc ggggcgatct cgaggatgac
     1261 gcccgcgcgt gggagcacgg cgtcaacgag ctggccgagt ccgacgagga ggtcgccgag
     1321 tacgtcacgt ccctggagca ggagaccgat acggttgacc tgcccgaggc ctccggtgac
     1381 gcgatagccc gcgagttcga gcagtacttg cgccggcgcg gcgagccaga cccgccccgt
     1441 cccggcggct agaggcggac gccgagcagg gcgtcgacgg tgcgcgcggc caggccgggg
     1501 gcccccgggt catcaccgcc atcggtcaga catgacgcgg cccacgcgtc gaccgccgcc
     1561 agggcgcctg cgccgtcgag gtcgtcaccg aggcgctcgc gcaccatcgc caccatcgcc
     1621 tccgcagccg ggcccgcgtt gaccgacaag gcggcccgcc acgtgtcgag gcggtgctgg
     1681 gcctcgacga gcaagggatc ggtccacgac cattcggtgc ggtagtgccg gcccagcagc
     1741 gcgagccgga tcgccatcgg gtccacgccc tggcggcgca gctcggagac gagcacgagg
     1801 ttgcccttgg acttgctcat cttctcaccg tcgaggccga ccatcgcctg gtggacatag
     1861 ctggacgcga acgtccgctc ccccgtcacg gcctcggcct gcaccgcgct catctcgtga
     1921 tgcgggaaga cgaggtcgct gccgccgccc tggatcgtga accccatccc gaggtagtcc
     1981 agcgcgatcg tcgtgcactc gatgtgccac ccaggccgtc cgaagccgag cgagccgccc
     2041 tcccaggccg gctcgccctc gcgttcggct cgccacagca gtgggtcgag cgggtcgcgc
     2101 ttgccgctgc gcccggggtc acccccgcgc tgcgcgaaga gctcgagcat gtcgtcccgg
     2161 ctcaggtgcc ccacctcacc gaaggccgga gcggccgtga ggtcgaggta gacgtcgtgc
     2221 ccggtcccct cgtcgccggg aaccgccagc ccgtatgccg cgccgccggc gaccagcgcc
     2281 gccacgacgt ccacgatgcg cgggaccgac tcgacgacgc cgatgtagtg gtcgggggcg
     2341 atgaccccga gcgcggtcat gtcctcgcgg aacagggccg tctcccgcga ggcgaggtcg
     2401 cgccagtcgt gaccgtcacg gacggcgcgg gcgagcagcg ggtcgtcgac atcggtgacg
     2461 ttctggacgt agcggacctg gtgacccgcg tcgcgcaggg cgcgcccgac gaggtcgaac
     2521 gtcacgtagg tcgcggcatg gccgaggtgg gtcgcgtcat acggcgtgat cccgcacacg
     2581 tagaggccgg cctcccctgt cacctcgacc gggcggaccg cacgggcaga ggtgtcatag
     2641 aggcgcagca cgggcccggt gccggggact gcggggatgg acggggacgg ccaggagatc
     2701 acagtcgcag agcctagctc gtgacccggg ccgcccccgt gaggccgcga cgcgctacag
     2761 cgccggccag gggatcgagg gccacccggg cgtcggggcc ggatgcaccc cggtcgccag
     2821 caaccggcgc acccggccgg tcagcgcgcc gacgtcctcg cgcggcaggg ccacgagcag
     2881 gtcgcggtgc agcctcgacc cggcatcggc cagcgcctcg tcgagccggc cgagacgctc
     2941 gaggtcggcg ggggtgagcg gctgtccggc ccatccccac aggacggtcc gcagcttggg
     3001 ctcgacgtgg aaggacacgc catggtcgaa gccccacagc cggcccgccg cgtcgcgcac
     3061 gacgtgggag cccttgcggt cggagttgtt cgtcac
//
LOCUS       DATAIX010000595         2572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_134869, whole genome shotgun sequence.
ACCESSION   DATAIX010000595 DATAIX010000000
VERSION     DATAIX010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134869"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VHM65_08790"
     CDS             <1..505
                     /locus_tag="VHM65_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2705842.1"
                     /translation="AGRVAAACQDETTRHWLTGLPDPFDEDTALAYVEHARASTASGS
                     EVYWAVADVSTDALAGALTLMAAGAPDTREVGYWTHPDARGRGVMAEALRLAVRHARI
                     DPQDGGLGLRRLVLRAAADNVASQRVAEQAGFVRTGSGRQAQRLGDGRYTDMVGYDLL
                     CAEIAGP"
     gene            complement(558..794)
                     /locus_tag="VHM65_08795"
     CDS             complement(558..794)
                     /locus_tag="VHM65_08795"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEX2705843.1"
                     /translation="MTPEWGGGRSERHVDRPARCLPRRRVPRDRAFGGTVSGPSMMSL
                     VDVGMHLLLAQIGPVALAVTTSISINFLRMPTPR"
     gene            complement(791..2101)
                     /locus_tag="VHM65_08800"
     CDS             complement(791..2101)
                     /locus_tag="VHM65_08800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2705844.1"
                     /translation="MSSTTAPATATRVHRAWWVALVILAALVAAAGFRSSTGALIGDL
                     EAEFGWSRATTSGAVSANLVIYGLTAPFAAALMDRFGVRRTVALALVLVSVGTGLTTV
                     MTQAWQLWALWGLAVGVGTGALALVLGSVIANRWFETQRGLVTGIFSAASATGQLVFL
                     PGIAALAAGPGWRYSALVITVAALLVIPAVLLLVAEWPGSVGLTPYGAPPTCRAQPLP
                     LALPHESPARTALRVLRESARARSFWVLMGTFWICGWSTNGLIGTHFIPAAHDHGMPG
                     TTAAGLLALIGIFDIAGTLASGWLTDRVDPRKLLVAYYVLRGVSLLVVPSLLAPSVHP
                     NLFFFVVFYGLDWVATVPPTMALCRNEFGLTRSSVVFGWVFASHMVGAGVAASYAGWV
                     RQSTGDYHGAWVTAGLLCMAAGLACFVLPAHRRPPPSPRRRYPG"
     gene            complement(2098..>2572)
                     /locus_tag="VHM65_08805"
     CDS             complement(2098..>2572)
                     /locus_tag="VHM65_08805"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX2705845.1"
                     /translation="DYFRLRPEQARPAIAHLVATGELVPVEVEGWRRPAYLPAGTRIP
                     RRVGARALLSPFDSLIWTRERTRALFGFDYRLEIYVPAHLRVHGYYVLPFLLGERLVA
                     RCDLKADRAAGTLRVHRVHWEHAPDDDEGAALQGELDDLAAWLGLGSVTWHGAPQ"
BASE COUNT          419 a          890 c          984 g          279 t
ORIGIN      
        1 cgcgggccgg gtcgcggcgg cctgccagga cgagacgacg cggcactggc tgaccggctt
       61 gcccgacccc ttcgacgagg acactgcgct ggcctatgtc gagcacgccc gggccagcac
      121 cgcgtcgggc tcggaggtct actgggccgt cgccgacgtg agcacggatg cactggccgg
      181 cgcgctcacc ctgatggcgg ccggtgcgcc ggacacgcgc gaagtggggt actggaccca
      241 cccggacgcg cggggacggg gcgtcatggc cgaggccttg cgcctggccg tccgccacgc
      301 ccggatcgac ccacaggacg gcggtctcgg gctgcgccgc ctcgtgctgc gcgcggcggc
      361 agacaacgtg gcctcgcagc gggtcgccga gcaggcgggc ttcgtgagga ccgggtcggg
      421 ccgccaggcg cagcgcctcg gggacggcag gtacaccgac atggtcggct acgacctgct
      481 ctgcgccgag atcgctggtc cgtgagcgct gctgcccgag ccggcccgca ctgagcctcc
      541 cggcgggatc gagtaggtca ccgaggcgtc ggcatccgca ggaagttgat gctgatgctg
      601 gtcgtgaccg ccagcgccac agggccgatc tgggcgagga gcaggtgcat cccgacgtcc
      661 acgagcgaca tcatcgacgg cccggagacc gtgccgccaa atgcgcgatc tcggggaact
      721 cggcgtcgag gaaggcagcg agctggtcga tcgacatgac gttcgctgcg gcctccgccc
      781 cactcgggtg tcacccaggg tagcgacgac gaggtgatgg cggcgggcgc cggtgggccg
      841 ggagcacgaa gcaggcgagg ccggccgcca tacagagcag tcccgcggtg acccaggccc
      901 cgtggtagtc accggtcgac tggcggaccc agccggcgta tgacgccgca acaccggctc
      961 cgaccatgtg cgaggcgaac acccagccga agacgacgct ggaccgggtc agcccgaact
     1021 cgttgcggca cagggccatc gtcggtggca ccgtggccac ccagtccagg ccgtagaaga
     1081 cgacgaagaa gaacaggttc gggtggaccg acggcgcgag caggctcggg acgacgagca
     1141 gggacacccc gcgcaggacg tagtaggcga cgaggagctt gcgggggtcg acccggtcgg
     1201 tcagccagcc cgatgccagg gtcccggcga tgtcgaagat cccgatgagg gcgagcagcc
     1261 ccgcggccgt cgtcccaggc atcccgtggt cgtgcgcggc ggggatgaag tgcgtgccga
     1321 tgagcccgtt cgtcgaccac ccgcagatcc agaacgtccc catgagcacc cagaacgacc
     1381 gggcccgcgc cgactcacgc agcacccgaa gggcggtccg ggccggggac tcgtggggga
     1441 gagccagggg cagcggctgg gctctgcacg tcggcggcgc gccatacggc gtgagaccga
     1501 ccgagccggg ccattcggcg acgaggagga gcaccgcagg gatgacgagc aaagcggcga
     1561 cggtaatgac gagggcggag taccgccacc caggtccggc ggccagcgcg gcgatgccgg
     1621 ggaggaagac gagctgcccg gtggcgctcg cggccgagaa gatcccggtg acgagcccgc
     1681 gctgggtctc gaaccagcgg ttcgcgatga ccgacccgag gacgagcgcc agcgccccgg
     1741 tgccgacccc gaccgcgagg ccccagagcg cccagagctg ccaggcctgg gtcatgaccg
     1801 tcgtgagccc ggtgccgacc gagacgagca ccaacgcgag ggcgaccgtc cggcgcacgc
     1861 cgaagcggtc catgagcgcg gcggcgaagg gcgcggtgag cccatagatg acgaggttgg
     1921 ccgagaccgc gcccgaggtc gtggcccgtg accagccgaa ctcggcctca agatcgccga
     1981 tgagcgcccc ggtcgaggac cggaagcccg ccgccgcgac gagggcagcg aggatgacca
     2041 gggccaccca ccaggcgcgg tgcacccggg tggccgtcgc cggtgccgtc gtggagctca
     2101 ctggggtgcg ccgtgccagg tcaccgagcc cagcccgagc caggccgcga gatcgtcgag
     2161 ctcgccttgg agcgccgcgc cctcgtcgtc atcgggcgcg tgctcccagt ggacccggtg
     2221 gacccgaagg gtgcccgcgg cccggtcggc cttgaggtcg caccgcgcga cgagccgctc
     2281 cccgagcagg aacggcagca cgtagtagcc gtggacacgc aggtgggccg gcacgtagat
     2341 ctccagccgg tagtcgaagc cgaacaaggc cctggtccgc tcgcgcgtcc agatgagcga
     2401 gtcgaacggg ctgagcaggg cccgcgcgcc gacccgtctc gggatcctcg tgcccgcggg
     2461 caggtaggcc gggcggcgcc agccctcgac ctcgaccggg acgagctcgc cggtcgcgac
     2521 gaggtgcgcg atggcggggc gcgcctgctc cggccggagc cggaagtagt cg
//
LOCUS       DATAIX010000596         1813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_134934, whole genome shotgun sequence.
ACCESSION   DATAIX010000596 DATAIX010000000
VERSION     DATAIX010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134934"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VHM65_08810"
     CDS             <1..622
                     /locus_tag="VHM65_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705846.1"
                     /translation="STRITGELSQLAMTSAAVNVAVRQRPDREGLSVGGEPVRATRTG
                     IDDVEIELRANSGDTPRSVAKAASGGELSRVMLAIEVVCSAGGADAAVPTMVFDEVDA
                     GVGGRAALSVGARLAELARHTQVIVVTHLAQVAAFGDQHLVVRKGDDGSVTSSSVTRV
                     AGDARLRELARMMSGDDHSEAGLVHAAELLGQAASIRDGPVPARTG"
     gene            656..>1813
                     /gene="steA"
                     /locus_tag="VHM65_08815"
     CDS             656..>1813
                     /gene="steA"
                     /locus_tag="VHM65_08815"
                     /inference="COORDINATES: protein motif:HMM:NF040608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HEX2705847.1"
                     /translation="MAFLTRRGRTPDPNPLRGAGVVRVDARTKNLTQRLRPGDIAVID
                     HIDIDRVSAEALVACKPAAVVNAASSISGRYPNLGPGILVGAGVPVIDNVGSAVMSAV
                     REGQRGEVAGGSLLVDGTEVASGVVLDAGLVEEAMTVARAGLSDQLEAFVVNTMDYLR
                     DERDLLFDGVGVPDIATELTGRHALIVVRGYHYREDLQTLRPYIRDYRPVLIGVDGGA
                     DAILEAGHTPDLIVGDMDSVSDSALRCGAEVVVHAYRDGRAPGLERVQSLGITPVVFP
                     ATGTSEDVAMLLADEGDAELIVAVGSHATLIEFLDKGRPGMASTFLPRLRIGGKLVDS
                     KGVSRLYRSRISTLTLAVMVLVGLLALLAALGATQSGRAALGVVAARWDDFV"
BASE COUNT          238 a          634 c          675 g          266 t
ORIGIN      
        1 gtccacgcgc atcaccggcg aactgtccca gctcgcgatg accagcgccg cggtcaacgt
       61 cgcggtccgg cagcgcccgg accgcgaggg gctcagcgtg ggcggggagc cggtccgagc
      121 cacgcgcacc ggcatcgacg acgtcgagat cgagctgcgc gccaactcgg gtgacacccc
      181 ccgcagcgtg gccaaggcag cgtcgggcgg cgagctgtcc cgggtgatgc tggcgatcga
      241 ggtcgtctgc agcgcaggcg gggcggacgc ggccgtcccg acgatggtct tcgacgaggt
      301 cgacgcgggg gtcggtggcc gcgcggcgct gtccgtcggc gcccgcctgg ccgagctggc
      361 gcgccatacc caggtgatcg tcgtgaccca cctcgcgcag gtggccgcgt ttggcgacca
      421 gcacctggtg gtccgcaagg gcgacgacgg gtcggtgacc tcgagcagcg tgacgcgggt
      481 cgccggggac gctcggctgc gtgagctggc ccggatgatg agcggggacg accactccga
      541 ggcgggcctc gtccatgccg ctgagctgct gggccaggcc gcctcgatcc gcgacggccc
      601 ggtccccgcc cgcaccggct aagtcctggc cgccgacatg acacgatggt ccgtgatggc
      661 attcctcacg cgccgaggcc gaaccccgga cccgaacccg cttcgcggcg ccggcgtggt
      721 tcgcgtcgac gcgcggacga agaacctcac ccagcggctg cgcccgggtg acatcgcggt
      781 catcgaccac atcgacatcg accgggtcag cgccgaggca ctggttgcgt gcaagcccgc
      841 cgcggtggtc aacgcggcga gctcgatctc cgggcgctac cccaacctcg gcccggggat
      901 cctggtcggc gccggtgtgc cggtcatcga caatgtcggg agcgccgtga tgtccgccgt
      961 gcgtgaaggc cagcgtggcg aggtcgccgg agggtcgctg ctcgtcgacg ggaccgaggt
     1021 ggcctcggga gtcgtgctcg acgcggggct cgtcgaggaa gcgatgacgg tggcccgggc
     1081 ggggctgtcc gaccagctcg aggcgttcgt ggtgaatacg atggactacc tgcgtgatga
     1141 gcgggacctg ctcttcgacg gtgtgggggt gccggacatc gccaccgagc tcacgggccg
     1201 gcacgcgctg atcgtcgtgc gcggctatca ctaccgcgag gacctgcaga cgctgcgccc
     1261 ctatatccgg gactaccggc cggtgctcat cggggtcgac ggcggagccg atgcgatcct
     1321 cgaggccggc cacaccccgg atctcatcgt cggtgacatg gactcggtct cggactcggc
     1381 cctgcgctgc ggcgccgagg ttgtcgtcca cgcctaccgt gacgggcgcg ctcccggcct
     1441 ggagcgggtc cagtcgctgg ggatcacgcc ggtcgtcttc cccgcgacgg ggaccagcga
     1501 ggatgtcgcc atgctgctgg ccgacgaagg cgacgccgag ctcatcgtgg ccgtcggctc
     1561 ccacgcgacc ctcatcgagt tcctcgacaa gggccggccc ggtatggcga gcacgttcct
     1621 ccctcgcttg cggatcggtg gcaagctcgt cgactccaag ggcgtgagcc ggctctaccg
     1681 gtcgcggatc tccaccctga cgctggccgt catggtcctc gtcggcctgc tcgcgctgct
     1741 cgcggccctc ggggcgaccc agtcggggcg ggcggcgctc ggggtcgtgg cagcgcgctg
     1801 ggacgacttc gtg
//
LOCUS       DATAIX010000597         2367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_284747, whole genome shotgun sequence.
ACCESSION   DATAIX010000597 DATAIX010000000
VERSION     DATAIX010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284747"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..811
                     /gene="fabI"
                     /locus_tag="VHM65_08820"
     CDS             50..811
                     /gene="fabI"
                     /locus_tag="VHM65_08820"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF005908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX2705848.1"
                     /translation="MGILQGKKLLVTGVLMDSSIAFHVARLAQDEGAEVLLTSFGRAM
                     KITTAIARRLPAPAEVIELDVTSTEDLDSLADRARVHLPRIDGVLHSIGFAPPGAFTF
                     MEASWEDVSVAMQASAYSLKALAQAALPLMSEGGSVVGLTFDARYAWPLYDWMGVAKA
                     AFEATSRYCARDLGPKGIRCNLVAAGPIRTTAAKSIPGFEAFERMWVRRAPIGWDVND
                     PVPAARACVALLSDWFPATTGEIVHVDGGVHATGG"
     gene            818..>2367
                     /locus_tag="VHM65_08825"
     CDS             818..>2367
                     /locus_tag="VHM65_08825"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX2705849.1"
                     /translation="MTDEGAAPPADMGVREIEVRLLDGPNLYFPKPALKVSLALAAYA
                     TLGPREARALARSIGQSGGRPGRPGSETRQRWLMRLVRYVVRRLARESGVTRIAVRTR
                     QGGALDEVVVALPWRHRTRARVTGEALAPLLRRVHADGVDVDEVFRVAAAAVAGADAG
                     PPPAVIGPSVPVVSITGTNGKTTTTRMIGHIVMTAGLTAAWSSTDGVVVMGEVVEPGD
                     YSGPAGARAVLQTPGLDIAILETARGGMLLKGMGVTANDVSVVTNVSADHLGLQGIDT
                     LDQLAEVKAIVTTVTKAGGWAVLNGEDPRVWAMRARTKAQPWAFALHADAPALREAVN
                     AGGRGITVLDGDIVVLYPDGRTDRLAPVVDVPLTLAGLSEHNIANCLAAAAAALALGI
                     ERDAVVEGLRTFAPDPTLNSGRMNTYTFALTGADEGGRESATVVVDMAHNEAGLEALL
                     AVCAGLRAPGGRTLLALGAAGDRSGEALSAMGELAVRGAEIVVIAHKERYLRGRTVEE
                     LSTLLRAG"
BASE COUNT          321 a          855 c          871 g          320 t
ORIGIN      
        1 ctgaccgact gtctcaccca gaacccccga cgagaggcac gacgcacgca tgggaatcct
       61 gcaaggcaag aagctgctcg tgacgggagt cctcatggac tcctcgatcg ccttccacgt
      121 cgcccggctc gcgcaggacg aaggcgccga ggtgctgctc acgtcgttcg ggcgggccat
      181 gaagatcacg acggcgatcg ccagacggct gccggctccc gccgaggtca tcgagctcga
      241 cgtgacgagc accgaggacc tcgactccct cgccgaccgg gcgcgcgtgc acctgccgcg
      301 gatcgacggt gtgctgcact cgatcggctt tgcgccaccg ggtgccttca ccttcatgga
      361 ggccagctgg gaggacgtgt ccgtcgcgat gcaggcgtcg gcatactccc tcaaggccct
      421 ggcgcaggca gcgctgccgc tcatgagcga gggtgggtcg gtcgtcggtc tcaccttcga
      481 cgcccgctat gcctggccgc tctacgactg gatgggggtg gccaaggccg ccttcgaggc
      541 gacgagccgc tactgcgccc gcgacctcgg ccccaagggg atccggtgca acctcgtcgc
      601 cgccgggccg atccggacga cggccgccaa gtcgatcccg gggttcgagg cgttcgagcg
      661 catgtgggtg cgccgggccc cgatcggctg ggacgtcaac gacccggtgc ctgccgcccg
      721 ggcctgcgtc gcgctgctgt cggactggtt cccggccacg acgggcgaga tcgtccacgt
      781 cgacggcggc gtccacgcga ccggggggta ggccccggtg accgacgagg gcgctgcgcc
      841 acccgctgac atgggtgtgc gtgagatcga ggtccgcctg ctcgacgggc cgaacctcta
      901 cttccccaag cccgcgctca aggtgagcct cgctcttgcg gcctacgcga cgctcggccc
      961 ccgggaggcg cgcgccctgg ccaggagcat cgggcaaagt ggcggacgcc cgggccgacc
     1021 ggggagcgag acccgccagc gatggctcat gcgcctggtc cggtatgtcg tccgtcggct
     1081 cgcgcgcgag tccggagtga cccggatcgc ggtgcgcacc cgccagggtg gcgcgctgga
     1141 cgaggtcgtc gtggcgctgc cgtggcgcca ccggacccgg gcccgcgtca ccggggaggc
     1201 gctggcgccc ctgctccggc gggtccacgc tgacggggtg gacgtcgatg aggtgttccg
     1261 cgtcgccgcg gcggccgtcg cgggcgcgga cgctggcccc ccgccggccg tcatcggccc
     1321 gtcggtccct gtcgtgtcga tcacgggaac caacggcaag acgacgacga cccggatgat
     1381 cgggcacatt gtcatgaccg ctgggctgac ggcggcctgg agctcgaccg acggggtcgt
     1441 cgttatgggc gaggtggtcg agccggggga ctactcgggc ccggccggtg cccgagccgt
     1501 cctgcagacc cccgggctcg acatcgccat cctcgagacg gcccgcggcg ggatgctcct
     1561 caagggcatg ggcgtgacgg ccaacgacgt cagcgtcgtc accaacgtca gcgccgacca
     1621 tctcggcctg cagggcatcg acaccctcga ccagctcgcg gaggtcaagg ccatcgtgac
     1681 gacggtgacc aaggcgggag gctgggccgt gctcaacggc gaggacccgc gggtgtgggc
     1741 catgcgcgcc cgcaccaagg cccagccgtg ggccttcgcg ctgcatgcgg acgccccggc
     1801 cctgcgcgag gcggtcaacg cgggcgggcg cgggatcacc gtcctcgacg gggacatcgt
     1861 cgtgctctac ccggacggcc ggaccgaccg gctcgccccg gtcgtcgacg tgccgctgac
     1921 gctcgccggg ctctccgagc acaacatcgc caactgcctc gccgcggctg cggcggccct
     1981 ggccctgggg atcgagcgcg acgcggtcgt cgaggggctg cggaccttcg ctccggaccc
     2041 gacgctgaac tccggccgga tgaacaccta caccttcgca ctgaccggcg ccgacgaggg
     2101 cggccgcgag agcgccacgg tcgtcgtcga catggcccac aacgaggcgg ggctggaggc
     2161 gctgctcgcg gtctgtgcgg gcctgcgcgc cccgggcggg cgcacactgc tcgcgctcgg
     2221 cgccgcgggg gatcgctccg gcgaagccct gtccgccatg ggggagctgg cggtccgcgg
     2281 ggccgagatc gtcgtcatcg cccacaagga gcgctacctg cgcggccgga ccgtcgagga
     2341 gctgagcacg ctgctccggg cgggtat
//
LOCUS       DATAIX010000598         1534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_284797, whole genome shotgun sequence.
ACCESSION   DATAIX010000598 DATAIX010000000
VERSION     DATAIX010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284797"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..>1534
                     /locus_tag="VHM65_08830"
     CDS             184..>1534
                     /locus_tag="VHM65_08830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2705850.1"
                     /translation="MPPAFPECVPVLTDGVVTLRAHQPQDADRIAEQCQDRLTWRFTT
                     VPHPYGPQDAHSFLGHVQDAWNDPAGSRYWAVTDATDAFHGSLDLRFDNDGRASIGVA
                     PHPQAWGRGLMARAVRLACGWAFAHGTEVIHWAAVAGNWASRRVAWACGFTMHGLVPG
                     LLGSGGRGVHDGWLGTLRRTDAMTPQGHWFDPPVLQGASLRLRPWRDDDAGHLVDPDA
                     DAQRFMPWGAAPFVADFAGWMLARDTRMADGEGIYWCIADRDTDRPAGHLQLFRLDQT
                     MSAGSAEVGYWLLPRERGRARMGEALGLLRGFAFGSPGLGGLGLHRLAAGTDAANTAS
                     NAVLRRAGFTQIGVEHEIMAGPDGTHSDGLLWELMAGADAPGGDQAPTIEGTAVRLRS
                     WRRTDAGGIVQACTDAQTRHWLAGLPDPYDEDTALAYVEHARACTASGAAVYWAVAAM
                     "
BASE COUNT          182 a          577 c          573 g          202 t
ORIGIN      
        1 cccaacggtc cgtgccgtcg cgcggttcct cccggccgcc ggcccgagct cactaccccg
       61 ttcgcctccg tatgccgtgc ccgcggtggg cgggcacggc atacgctccc ggaagcgcga
      121 gcccgggccg gctgccgacg atccccgggc ctggcaggcc cggcaagtgg cagggtgtgc
      181 cgtatgccgc ccgccttccc cgagtgcgtg cccgtcctca ccgacggggt cgtcacgctg
      241 cgggcccacc agccgcagga cgcggatcgc atcgccgagc agtgccagga ccggctgacg
      301 tggcgcttca cgaccgtccc gcacccctac ggaccgcagg acgcgcactc gttcctcggt
      361 cacgtccagg acgcatggaa cgacccggcc gggtcccgct actgggcggt caccgacgcg
      421 accgacgctt tccacggctc gctcgacctg cgcttcgaca acgacgggcg ggcgtcgatc
      481 ggcgtcgcgc cgcacccgca ggcgtggggg cggggtctca tggcacgagc ggtccggctg
      541 gcctgcgggt gggcgttcgc ccacggcacc gaggtcatcc actgggcagc ggtcgccggc
      601 aactgggcgt cgcggcgggt ggcgtgggcg tgcggcttca ccatgcacgg cctcgtgcct
      661 ggcctgctcg gctccggcgg gcgcggcgtg cacgacgggt ggttgggcac gctccgccgc
      721 accgacgcga tgacccccca ggggcactgg ttcgacccgc ccgtcctgca gggcgcgtca
      781 ctgcggctcc gaccgtggcg cgacgacgac gccgggcacc tcgtcgaccc tgacgccgac
      841 gcccagcggt tcatgccctg gggcgccgcg ccgttcgttg cggacttcgc cggctggatg
      901 ctcgcgcggg acacccggat ggccgacggt gaggggatct actggtgcat cgccgaccgt
      961 gacaccgacc ggcccgcggg tcacctgcag ctcttccgcc tggaccagac catgtccgcc
     1021 gggagtgccg aggtgggcta ctggctgctc ccccgcgagc gcgggcgggc ccggatgggc
     1081 gaggcgctcg ggctgctgcg gggcttcgcc ttcgggtcgc ccggcttggg gggcctcggg
     1141 ctgcaccggc tcgccgccgg gaccgacgcg gccaacaccg cctcgaacgc cgtgctgcgc
     1201 cgagccgggt tcacccagat cggtgtcgag cacgagatca tggccggccc ggacggcacc
     1261 cactccgacg ggctgctctg ggagctcatg gccggcgcgg acgcgccagg tggcgaccag
     1321 gcgccgacga tcgaggggac ggccgtgcgg ctgcggtcat ggaggcgcac ggatgcgggc
     1381 gggattgtgc aggcctgcac ggacgcgcag acgcggcact ggctggccgg actgcccgac
     1441 ccctacgacg aggacaccgc gctcgcctat gtcgagcacg ccagggcctg cacggcgtcg
     1501 ggcgcggcgg tctactgggc cgtcgccgcc atga
//
LOCUS       DATAIX010000599         1718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_224796, whole genome shotgun sequence.
ACCESSION   DATAIX010000599 DATAIX010000000
VERSION     DATAIX010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224796"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..1367)
                     /locus_tag="VHM65_08835"
     CDS             complement(195..1367)
                     /locus_tag="VHM65_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705851.1"
                     /translation="MTVSTSQRELAVGIGAAGLATADMVLNIGPQHPATHGVLRLRIV
                     LDGERIVSAEPVIGYMHRGAEKLFEVRDYRQILTLANRHDWLSAFANELGVALGVETM
                     LGMEVPVRAVWARTLLAELNRVLSHLMFLGSYPLELGAITPVFYAFREREEIQAVLEE
                     ASGGRMHYMFNRIGGLKEDLPAGWLARTAAAITAVRARMPDIDDLLLGNEILHARTKG
                     VGVLRPETVLEYGVSGPIARASGVDLDLRRDAPYLAYGELFVPGGPGRVVTRTEGDGF
                     ARLECLLEQTYVSLDIAEACVDKLASLPQGPINVRLPKVLKVPEGHLYTATENPLGFN
                     GYYLVSKGEKTPWRLKLRSASFGNVQVLSVMMPGQLVADMVSILGSMFFVVGDVDK"
     gene            1511..>1718
                     /locus_tag="VHM65_08840"
     CDS             1511..>1718
                     /locus_tag="VHM65_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705852.1"
                     /translation="MTFDAAELLDLADPAVVADPYTHYRALRGRAPVAWHSGLGLYIA
                     TSHAACNQLLRDRSLGRIVAPRLPE"
BASE COUNT          256 a          586 c          635 g          241 t
ORIGIN      
        1 ggccgcgcgg ccccaagggg ctcaggtgag atctcatcgc ttggccggtc ggcaccggcc
       61 ggatccacct cggtagtccg ggggtggctc acagaggtgg ttccggccgg tcagggccgg
      121 ccagatcggc ctcggtagtc cgggggcggc tcactgaggt ggttccggcc ggcccgggct
      181 gacaccgggg gcgactactt gtcgacgtct ccgacgacga agaacatcga gccgaggatc
      241 gagaccatgt cggcgacgag ctggcccggc atcatcaccg agagcacctg gacgttgccg
      301 aaggaggccg agcgcagctt gagccgccac ggcgtcttct cgcccttgga gacgaggtag
      361 tagccgttga acccgagcgg gttctcggtc gccgtgtaga ggtggccttc gggcaccttg
      421 agcaccttgg gcagcctgac gttgatcggc ccctgcggca gcgaggcgag cttgtcgacg
      481 cacgcctcgg cgatgtcgag gctgacgtag gtctgctcga gcaggcactc cagccgggcg
      541 aacccgtcgc cctccgtgcg ggtcaccacg cggccgggtc cgccggggac gaacagctcg
      601 ccataggcga ggtatggcgc gtcccggcgc aggtcgaggt cgacgccgct ggcccgcgcg
      661 atgggacccg agacgccata ctccagcacg gtctccggtc gcaggacgcc gacccccttg
      721 gtgcgggcgt gcaggatctc gttgccgagc aggaggtcgt cgatgtcggg catgcgggcg
      781 cgcacggccg tgatcgccgc ggcggtgcgg gcgagccagc cggcggggag gtcctccttg
      841 agaccaccga tccggttgaa catgtagtgc atccgcccac ccgacgcctc ctcgagcacc
      901 gcctggatct cctcgcgctc acggaacgcg tagaagaccg gcgtgatcgc gccgagctcg
      961 agcgggtagg acccgaggaa catgaggtgg ctgagcaccc ggttgagctc ggcgagcagc
     1021 gtgcgcgccc agaccgcccg gacgggcacc tccatgccga gcatcgtctc gacaccgagc
     1081 gcgacgccga gctcgttggc gaacgccgag agccagtcgt gccggttggc cagggtgagg
     1141 atctggcggt agtcacggac ctcgaagagc ttctcggcgc cgcggtgcat gtagccgatg
     1201 accggctccg cgctcacgat gcgctcaccg tcgaggacga tgcgcaaccg gagcacgccg
     1261 tgcgtcgcgg ggtgctgcgg gccgatgttg aggaccatgt cggctgtggc gagcccggcc
     1321 gcgccgatgc ctacggcgag ctcgcgctgg gaggtgctga ccgtcatacg gatgagtctg
     1381 ccgtatgacg cgccgagcgc cacctaccaa aggtcacggg cgtgccggag ccggcccgga
     1441 ccgggtgcca cggcccggaa accagtgagc agccccgggg ggcggctcga gtagggcagg
     1501 ctgggcgtcc atgacgttcg acgccgccga gctgctcgac ctggccgacc cggctgtggt
     1561 cgccgacccg tacacgcact accgggcgct gcggggccgc gcgcccgtgg cctggcacag
     1621 cgggctcggg ctgtatatcg cgacgagcca cgccgcgtgc aaccagctcc tgcgggaccg
     1681 cagcctcggg cgcatcgtcg ccccccggct cccggagc
//
LOCUS       DATAIX010000600         1842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_195362, whole genome shotgun sequence.
ACCESSION   DATAIX010000600 DATAIX010000000
VERSION     DATAIX010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195362"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VHM65_08845"
     CDS             complement(<1..425)
                     /locus_tag="VHM65_08845"
                     /inference="COORDINATES: protein motif:HMM:NF022676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical enzyme domain-containing protein"
                     /protein_id="HEX2705853.1"
                     /translation="MLQFETMASAITRDPATGYDQKVRRLAALALSVLPYPALSADCC
                     EALDKRVICDMYEGNTPFTPRYVLPDYARAMRQGSPHLELDPPRDLDEALSFLTIMYG
                     HVPSVTTYPVYLGDLDSLLEPYVGDLTDAELDRRLRRFW"
     gene            522..>1842
                     /locus_tag="VHM65_08850"
     CDS             522..>1842
                     /locus_tag="VHM65_08850"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX2705854.1"
                     /translation="MPSLDDLLHAAVAGIAGVERPGQGQMARAVAAAVESGEHLLVQA
                     GTGTGKSLAYLVPAVVHAVNTGEPTIVATATLALQAQIIDRDLPRLADSIAPLLGRRP
                     TYALVKGRRNYLCAHKLEGGFPDEDDSLFGVEQVDRAASRLGAEMVRLRAWAGETASG
                     DRDELMPGVSERAWRQVSVSAHECLGSRCPMVSECFVERSREAARDVDVVVTNHSFMA
                     IDAFEGRQMLPPHDLLVIDEAHELTDRVTSTITDELTEAMCLAAARRCAKLADSHGTL
                     EEAAVLLGQALDATPEGRLTGIPDAVAMALARVRDTSRALQSELKPASPELADGARQV
                     ARAAVEEVYEVSARVLEERELDVVWVTHEQRRGSVLRVAPMSVATLVRERVFTGRTVV
                     LTSATLELGGTFDAVAGTIGLRGDGAPSWQGLDVGSPFDYPQQGIAYV"
BASE COUNT          259 a          654 c          669 g          260 t
ORIGIN      
        1 atccagaagc ggcgcagcct gcggtcgagc tcggcgtcgg tgaggtcgcc gacatacggc
       61 tcgagcaggg agtcgaggtc gccgagatag acggggtatg tcgtcacgga cgggacgtga
      121 ccgtacatga tcgtgaggaa ggacaaggcc tcgtcgagat cacggggcgg gtcgagctcg
      181 aggtgagggc tcccctggcg catggccctg gcgtagtccg gcaggacgta gcgcggggtg
      241 aagggcgtgt tgccctcgta catgtcgcag atgacgcgtt tgtccagagc ctcacagcag
      301 tcggcggaca aggcggggta gggcagcaca ctcagcgcga gcgcggcgag ccggcgaacc
      361 ttctggtcgt acccggtggc ggggtctcgc gtgatggccg acgccatggt ctcgaactgg
      421 agcatctcca cccgtccagg gtagtcccgc ggtgtgagcg agcccgccgt gatgagcccc
      481 ggcgtaggtg ggcgcgcccg cctgacccgt agggtgtacc aatgccctcg ctcgacgacc
      541 tgcttcacgc ggccgtcgcc gggatcgcgg gtgtcgagcg gcccggacag ggccagatgg
      601 cccgcgccgt cgctgccgcc gtcgagtcgg gcgagcacct gctcgtccag gccggcaccg
      661 gcaccggcaa gtcgctggcc tacctcgtgc ccgcggtcgt ccacgcggtc aacaccgggg
      721 agcccacgat cgtggccaca gccaccctgg ccctgcaggc ccagatcatc gaccgggacc
      781 tgccccgcct cgccgactcc atcgccccgc tgctcggccg ccggccgacc tacgccctgg
      841 tcaagggccg gcgcaactac ctctgcgcgc acaagctcga ggggggcttc cccgacgagg
      901 acgactcgct cttcggggtc gagcaggttg accgggccgc ctcccgcctc ggcgccgaga
      961 tggtccggct acgcgcctgg gccggcgaga ccgcctcggg cgaccgggac gagctcatgc
     1021 ccggcgtgtc cgaacgggcc tggcggcagg tgtcggtctc ggcccacgag tgcctcggct
     1081 cgagatgccc gatggtcagc gagtgcttcg tcgaacgctc ccgtgaggcc gcccgcgacg
     1141 tcgacgtcgt cgtgaccaac cactcgttca tggcgatcga cgccttcgag ggccgccaga
     1201 tgctcccgcc gcacgatctg ctcgtcatcg acgaggccca cgagctcacc gaccgggtca
     1261 cctcgacgat caccgacgag ctgaccgagg cgatgtgcct cgcggcggct cggcgctgcg
     1321 ccaagctcgc ggactcgcac gggaccctcg aggaggctgc ggtcctgctc ggccaggcac
     1381 tggacgcgac cccggagggc cggctgaccg gcataccgga tgccgtggcg atggccctcg
     1441 cccgggtgcg cgacacgtct cgggcgctgc agagcgagct caagcccgcc tcgcccgagc
     1501 tggccgacgg cgcgcgtcag gtggcccggg ccgcggtcga ggaggtctac gaggtctccg
     1561 cgcgggtcct cgaggagcgc gagctcgacg tcgtgtgggt cacccacgag cagcgccgcg
     1621 gctcggtcct gcgggttgcg ccgatgagcg tggccacgct cgtgcgcgag cgggtcttca
     1681 ccggccgcac cgtcgtcctc acctcggcca cgctcgagct gggcggcacc ttcgacgcgg
     1741 tcgcgggcac gatcgggctg cgcggcgacg gggcaccgtc atggcaggga ctggacgtcg
     1801 gcagcccgtt cgactacccc cagcagggca tcgcgtatgt ct
//
LOCUS       DATAIX010000601         4174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_254247, whole genome shotgun sequence.
ACCESSION   DATAIX010000601 DATAIX010000000
VERSION     DATAIX010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254247"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VHM65_08855"
     CDS             complement(<1..573)
                     /locus_tag="VHM65_08855"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705855.1"
                     /translation="MVHPRAGTVALPEDLVDIGALVDAYYDRSPDVSNPDEQVVFGTS
                     GHRGSSLKTAFNEAHILATTQAICEYRTSVGVDGPLFIGRDPHGLSEPAWRSALEVLG
                     ANGVTVFVDSRDGFTPTPAISHAILRANRGKAGPGAGANGLADGIVVTPSHNPPADGG
                     FKYNPTHGGPADTDATSWIARRANEIIAAGM"
     gene            complement(586..2817)
                     /gene="glgB"
                     /locus_tag="VHM65_08860"
     CDS             complement(586..2817)
                     /gene="glgB"
                     /locus_tag="VHM65_08860"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX2705856.1"
                     /translation="MTEVPVTASVMDNTAIDAFVEGRHSQPHDLLGHHQGPGGLTVTA
                     YRPLARSVRVRLADGSLLVLRHLRDGVWSGTAGALTSTQDYRLLVSYDDGIEHVQDDP
                     YRFAPTLGEVDRHLINEGRHEQLWTVLGAHVREYGGPLGTVYGTSFAVWAPNATAVHV
                     IGDFNGWDRITHPMRILNPSGIWELFIPGVGEGTKYQFLVRGADAVVRVKTDPMARAT
                     EVAPSTASIVTQSHYDWGDDGWLRSRTTNNPHNGPMSVYEVHLGSWRQGLSYLDLAEH
                     LVNYVHDLGFTHVELLPVMEHPYVPSWGYQVTGYYSPTGRFGSPDEFRHLVDRLHQAG
                     IGVILDWVPAHFPKDDWALARFDGQPLYEYGDPSRGEHPDPRKGDQPDWGTYVFDFGR
                     SEVRNFLVANAIYWLEEFHIDGLRIDAVASMLYLDFSRRPGEWVPNAYGGRENLEAMH
                     LLQEVNATAYKRVPGIVMIAEESTAWPGVTGSTADGGLGFGFKWNMGWMNDSLRYLGQ
                     DAIYRQYHHQELTFALIYAFSENFILPISHDEVVHGKGSLLRKSRGSRDEQLATLRAY
                     LSYLWTHPGKQLIFMGSEFGQESEWAEGRSLDWWLLEQPAHYRVHALVKDMNAIYRSH
                     RQLWELDSSGAGFQWIDADDAGRNTVSYLRFGTGDRATDAPVLAAVINFSGQTYYDYP
                     IGLPRGGRWKVVLDTAGYRPEAPSSTGVVHEARSEGYAGQPYSISIIAPALSGVWLVP
                     DES"
     gene            complement(2814..>4174)
                     /locus_tag="VHM65_08865"
     CDS             complement(2814..>4174)
                     /locus_tag="VHM65_08865"
                     /inference="COORDINATES: protein motif:HMM:NF037041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705857.1"
                     /translation="TEHGALTQALADSLPGWVSTQRWYAGKGHLPQLRRIGARSWPDP
                     TGEAGIEVHLVLDTAGGRHTVYQMPFTIRRAPLAGAEQALVATRAGSAPDTNGATYVY
                     DGPHDPVYATALMAAIGGHEIAMTASRVLRGEQSNTSIIIDGLAPDGSAHPVIAKIYR
                     VLHHGENPDVIIQSALAAAGCDRVPAPIGSIEGSWPDASVDGGRARGHLAFVQEFLAG
                     TQDAWRVAAHALAEGSDFTTAAHALGAATAEVHQTLARALETREATPEDRRALVRTWR
                     TRHTTAVSEVPALAEHDAVIRAVYDEAGVAPWPALQRVHGDYHLGQVLDAPGRGWVLL
                     DFEGEPLRPLSERSQPDLALRDVAGMLRSFDYAAGAHVMAHPQDDELASAWAAACREA
                     FLDGYADVAADPRDEAVLLAALELDKALYEVVYEARNRPRWLPIPGQAVERIVASRKA
                     PG"
BASE COUNT          604 a         1385 c         1546 g          639 t
ORIGIN      
        1 catccccgcg gcgatgatct cgttggcgcg ccgcgcgatc cacgacgtcg cgtcggtgtc
       61 ggccggaccg ccgtgcgtcg ggttgtactt gaacccgccg tcggcgggag ggttgtgcga
      121 cggggtgacg acgatcccgt cggcgaggcc gttggcgccc gcgcccggac ccgccttgcc
      181 tcggttggct cgcaggatcg cgtgcgagat cgcgggcgtc ggggtgaacc cgtcgcgcga
      241 gtcgacgaac accgtcacgc cgttggcccc gagcacctcg agcgccgatc gccaggcggg
      301 ctcggacagc ccgtgcgggt cccggccgat gaacagcggc ccgtcgacgc cgaccgaggt
      361 gcggtactcg cagatcgctt gggtcgtggc caggatatgg gcctcgttga aggccgtctt
      421 gaggctcgac ccccggtgtc cggacgtacc gaagacgacc tgctcgtcgg ggttcgagac
      481 gtcgggcgac cggtcgtagt acgcgtcgac aagggcgccg atgtcgacga ggtcctctgg
      541 cagcgcgacc gttccggcac gggggtgaac catgacgcta ctccttcagc tctcgtccgg
      601 gacgagccag acgccggata gggccggggc gatgatcgag atcgagtaag gctgcccggc
      661 atacccctcc gagcgtgcct cgtggacgac accggtcgag ctcggcgcct cagggcggta
      721 cccggcggtg tcgaggacga ccttccagcg cccaccccgg ggcagcccga tggggtagtc
      781 gtagtacgtc tggccgctga agttgatgac tgcggcgagc acgggcgcat cggtggcgcg
      841 gtcgcccgta ccgaaacgca ggtaggacac ggtgttgcgg cctgcgtcgt cggcgtcgat
      901 ccactggaac ccggctccgg aggagtcgag ctcccagagc tggcggtgcg accggtagat
      961 ggcgttcatg tccttgacca gcgcatggac ccggtagtgc gcgggctgct ccagcagcca
     1021 ccagtccagg ctgcgccctt cggcccactc ggactcctgc ccgaactccg accccatgaa
     1081 gatgagctgc ttgccgggat gcgtccagag gtaggacagg taggcgcgca aggtggcgag
     1141 ctgctcgtcc cgggagccgc gcgacttgcg cagcagcgag cccttgccgt ggacgacctc
     1201 gtcgtggctg atcggcagga tgaagttctc gctgaatgcg tagatgagcg cgaaggtcag
     1261 ctcctggtgg tggtactggc ggtagatcgc gtcctggccg aggtagcgca gggagtcgtt
     1321 catccagccc atgttccact tgaagccgaa gccgagcccg ccgtcggccg tcgagcccgt
     1381 cacgcccggc caggccgtcg actcctcggc aatcatgacg atccccggga cccgcttgta
     1441 tgccgtggcg ttgacttcct ggagcagatg catcgcctcg aggttctccc ggccgccgta
     1501 ggcgttgggg acccactcgc cgggccggcg cgagaagtcg aggtagagca tcgaggcgac
     1561 tgcgtcgatc cgcagcccgt cgatgtggaa ctcctcgagc cagtagatcg cgttggcgac
     1621 gaggaagttg cgcacctcgg accggccgaa gtcgaacacg taggtgcccc agtccggctg
     1681 gtcgcccttg cgggggtcgg ggtgctcgcc gcgactgggg tcgccgtact cgtagagcgg
     1741 ctgaccgtcg aagcgggcga gggcccagtc gtccttgggg aagtgcgcgg gcacccagtc
     1801 gaggatgacg ccgatgccgg cctggtgcag ccggtcgacg agatggcgga actcgtcagg
     1861 gctgccgaac cgcccggtcg gcgagtagta gccggtgacc tggtagcccc acgacggcac
     1921 gtaagggtgc tccatgacgg gcaggagctc gacatgggtg aacccgaggt cgtggacgta
     1981 gttgacgagg tgctcggcca ggtcgaggta ggagagcccc tggcgccacg acccgaggtg
     2041 gacctcgtag acgctcatcg ggccgttgtg cgggttgttc gtcgtccgcg accgcagcca
     2101 gccgtcgtcg ccccagtcgt agtgcgactg cgtgacgatc gaggcggtcg acggcgcgac
     2161 ctccgtcgcc cgggccatcg ggtcggtctt gacccgcacg accgcgtcgg caccgcgcac
     2221 caagaactgg tacttcgtgc cctcccccac acctgggatg aacagctccc agatgcccga
     2281 ggggttcagg atgcgcatcg ggtgcgtgat ccggtcccag ccgttgaagt cgccgatgac
     2341 gtggacggcg gtcgcgttgg gcgcccacac cgcgaacgag gtgccataca ccgtcccgag
     2401 cgggccgcca tactcgcgca cgtgggcgcc gaggacggtc cagagctgtt catggcgccc
     2461 ctcgttgatg aggtgccggt cgacctctcc gagcgtcggg gcgaaccggt aagggtcgtc
     2521 ctggacgtgc tcgatcccgt cgtcgtagct gacgagcagg cggtagtcct gggtcgacgt
     2581 gagagccccc gcggtcccgg accagacccc gtcgcgcagg tgccgcagga ccaggagcga
     2641 gccgtcggcg aggcgcaccc gcacgctgcg tgccagtggc cggtaggccg tgaccgtgag
     2701 cccgccaggg ccctggtgat ggccgagcag gtcgtgcggc tggctatgcc gtccctcgac
     2761 gaacgcgtcg atagcggtgt tgtccatgac ggacgcggtg acaggaactt cggtcatcct
     2821 ggtgccttcc tcgatgcaac gatgcgttcg acggcctggc cggggatcgg cagccatctg
     2881 ggtcggttgc gggcttcgta gacgacctcg tagagcgcct tgtcgagctc gagcgcggcg
     2941 agcaggacag cctcgtcgcg cgggtccgcg gcgacgtccg cgtagccgtc gaggaacgcc
     3001 tcgcggcagg ccgccgccca tgcggaggcg agctcgtcgt cctgcgggtg ggccatgaca
     3061 tgggcccccg ccgcatagtc gaacgagcgg agcatcccgg cgacgtcacg cagggccagg
     3121 tcgggctggc ttcgctcgga cagcgggcgc aggggctcgc cctcgaagtc gagcaggacc
     3181 cagccgcgtc cgggcgcgtc gagaacctgg ccgaggtgat agtcgccgtg gacccgctgc
     3241 agtgcgggcc agggggccac accggcctcg tcgtagaccg cccggatcac tgcgtcgtgc
     3301 tcggcgaggg caggcacctc ggacacggca gtggtatggc gggtgcgcca ggtgcgcacg
     3361 agcgcgcgcc ggtcctccgg ggttgcctcg cgcgtctcga gggcccgggc cagggtctgg
     3421 tggacctccg cggtcgccgc gccgagggcg tgggccgccg tcgtgaagtc gctgccctcg
     3481 gccagggcgt gggcggcgac ccgccacgcg tcctgggtgc ccgcaaggaa ctcctggacg
     3541 aaggcgaggt gccctctggc ccggcccccg tcgacggagg cgtccggcca gctgccctcg
     3601 atcgacccga tgggcgcggg gacccggtcg cacccggcgg cggccaaagc gctctggata
     3661 atgacgtcag ggttctcgcc gtggtgcagg acccggtaga tcttggcgat cacggggtgt
     3721 gccgacccgt ccggagcaag cccatcgatg atgatcgagg tgttggactg ctcaccacgc
     3781 agcacccggc tggccgtcat cgcgatctca tggccgccga tggccgccat gagagcggtc
     3841 gcgtagaccg ggtcatgcgg accgtcgtag acgtacgtgg cgccgttcgt gtctggggcc
     3901 gagccggccc tcgtcgcaac gagagcctgt tcggccccgg ccagcggggc gcgccggatg
     3961 gtgaagggca tctggtagac cgtgtgccgc ccgccggcgg tgtcgagcac gaggtgcacc
     4021 tcgatcccgg cctccccggt cgggtccggc cagctgcgcg cgccgatccg ccgcagctgc
     4081 gggaggtgac ccttcccggc ataccagcgc tgggtcgaga cccagcccgg cagggagtcg
     4141 gcgagggcct gggtgagggc gccgtgctca gtca
//
LOCUS       DATAIX010000602         2516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_195451, whole genome shotgun sequence.
ACCESSION   DATAIX010000602 DATAIX010000000
VERSION     DATAIX010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195451"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..898
                     /locus_tag="VHM65_08870"
     CDS             62..898
                     /locus_tag="VHM65_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HEX2705858.1"
                     /translation="MSSVKQSFEQHMERAVPGDPSSEAPGKIVRAFGDTTGDGMVQLS
                     FTLPLPHDKRAEGAALQLARKMGMEPAMLVHAKAMGPSFTFFVIYGSVSHVVDLRQVK
                     VVEREFPLLSSKEVNTAIRSALRRRLVVVGACIGSDAHTVGIDAILNIKGFAGEKGLE
                     YYPEIRVVNLGAQVLVPELVARARLEQADAVLVSQVVTQKDAHIHNTTQMTAAFREAY
                     PARKVPVLVAGGPRFEEDAAARLGVDRIFGRGTTPSEVASYLVHAVTTRAIRRAARKA
                     SR"
     gene            895..1305
                     /locus_tag="VHM65_08875"
     CDS             895..1305
                     /locus_tag="VHM65_08875"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEX2705859.1"
                     /translation="MSSRPAAGAHAGMTVTHRRYVPYSHAHYAGNLVDGAYSLAAFGD
                     VATEMCIRTDGDEGLFASYSDVQFRAPVRAGDVIEIEARLVRVGSRSREMDFELRVVA
                     RGTPGRGESAAALLDPPVVATTARGVVVVPLPAE"
     gene            1828..>2516
                     /locus_tag="VHM65_08880"
     CDS             1828..>2516
                     /locus_tag="VHM65_08880"
                     /inference="COORDINATES: protein motif:HMM:NF039484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705860.1"
                     /translation="MRRALLALLAGGALTLSFPGSGIWLLAPVGVGALALATRGTSIR
                     TGACLGFLAGLAYFLPTLSWTGIYVGALPWVALAILQALYVAVVGAVSSALQQGVSVA
                     GADRVRPLVVALAWVGSEALRARTPYGGFPWARIAFTQADSPLNHIAALAGAPGVTFA
                     VALAGGVLASALAPRAALAAPARQRVLARPRWRRVAAAVLLVVSALAVPLHTEGRPAN
                     VMAVQGNVPHPG"
BASE COUNT          320 a          914 c          909 g          373 t
ORIGIN      
        1 tcgcccgcgc acccggctac gacaacccgg cgatcacctt gctcgagacg ggggcggacc
       61 gatgagcagc gtcaagcagt ccttcgagca gcacatggag cgcgccgtcc ccggcgaccc
      121 gagctcggag gcgcccggca agatcgtgcg cgccttcggt gacacgaccg gcgacgggat
      181 ggtccagctg tccttcaccc tgcccctgcc gcacgacaag cgggccgagg gggccgcgct
      241 gcagctggcc cgcaagatgg gcatggagcc ggcgatgctc gtgcacgcca aggcgatggg
      301 gccctcgttc accttcttcg tcatctatgg cagcgtgagc cacgtcgtgg acctgcgcca
      361 ggtcaaggtg gtcgagcgcg agttccccct gctgtcctcc aaggaggtca acacggccat
      421 ccgctccgcg ctgcgccgcc gtctggtcgt cgtcggtgcc tgcatcggca gcgacgctca
      481 cacggtcggc atcgacgcga tcctgaacat caagggcttc gcgggggaga agggcctgga
      541 gtactacccg gagatcaggg tcgtcaacct cggcgctcag gtgctcgtgc ccgagctcgt
      601 ggcgcgggcg cgcctggagc aggccgacgc ggtgctcgtg agccaggtcg tcacccagaa
      661 ggacgcccat atccacaaca cgacgcagat gaccgcggcc ttccgcgagg cctacccggc
      721 ccgcaaggtg ccggtgctcg tggccggtgg gccccgcttc gaggaggacg ctgccgcgcg
      781 gctcggggtc gaccggatct tcgggcgggg gaccaccccg agcgaggtgg ccagctacct
      841 cgtccatgcc gtcacgacac gcgcgatccg gcgcgcggcg aggaaggcgt cccgatgagc
      901 agccggcccg ccgcaggggc ccacgccggt atgacggtca cgcatcggcg gtatgtgcct
      961 tacagccacg cccactacgc gggcaacctc gtcgacggtg cctactcgct ggccgccttc
     1021 ggcgatgtcg ccaccgagat gtgcatccgc accgacggcg acgaggggct gttcgcgtca
     1081 tacagtgacg tccagttccg tgccccggtg cgcgcgggcg acgtgatcga gatcgaggcc
     1141 cgcctggtgc gggtcgggtc gcgcagccgt gagatggact tcgagctgcg cgtcgtggca
     1201 cgcgggacgc ccgggcgggg ggagtccgcg gccgcgctgc tcgacccgcc ggtcgtggcc
     1261 accaccgcgc gcggcgtcgt cgtggtgccc ttgcccgccg agtgagcggg cacccggggg
     1321 tgggtcggcc tgcgcggtga cggttgctcc ggcgtgtcgc gagcgacacg ccggagcaac
     1381 ccggcaaaag gcctcatccg gacaggtccg aacaccggcc tgaccagcga caacgcgcgt
     1441 ctgcccaggt cagaggccag ttgacacaga gacgaccaga ggtaggttgg acacgggttg
     1501 ttcaccgacg ggccatctca gggtggtcgt gggacagccc cgcaggggtc acatcgtggc
     1561 atctgcgctt ccgtcgtgca ttctgggccc gcgcaggggc tctggcggcg gcggagaatg
     1621 cgggagatga agggcggaag gcccgtcacg cctagtagac aacgcccgcg gcccggcatc
     1681 cagccggacc cgtgggtggt accaacggcg gggcgggtcc tctgtccccc agacgctgcg
     1741 cccggaccgg gcacccaacg gcggaccggc acggccctcg ggcgcgtccg gtgtgagctc
     1801 ggtccccggg ccggttaccg tgtccccgtg cgccgcgccc tcctcgccct gctggctggg
     1861 ggcgcgctga cgctgtcgtt tcccggatcg ggcatctggc tgctcgcccc tgtcggcgtc
     1921 ggcgcgctcg cgctcgccac ccgcgggacc tcgatccgca ccggcgcctg cctggggttc
     1981 ctcgccggac tggcctactt cctgcccacg ctgtcgtgga cggggatcta cgtcggagcg
     2041 ctgccctggg ttgcgctcgc catcctgcag gccctgtatg tcgctgtcgt cggcgccgtg
     2101 tcgtccgccc tgcagcaggg cgtgtcggtt gccggggcag accgcgttcg tcccctcgtc
     2161 gtcgccctcg cgtgggtggg gtccgaggcg ctgcgtgcgc gcacgccata cggtggcttc
     2221 ccctgggccc ggatcgcctt cacccaggcc gactccccgc tgaaccacat cgcggcgctg
     2281 gccggggccc ctggtgtcac ctttgcggta gccctggccg ggggggtgct cgcctccgcg
     2341 ttggctccgc gagcggccct cgcggccccc gcccgccagc gcgtcctcgc gaggccgcgc
     2401 tggcgccggg tcgctgccgc ggtgctgctc gtggtgtccg cgctggcggt accgctgcac
     2461 accgagggac gcccggccaa cgtcatggcc gtgcagggca acgtcccgca ccccgg
//
LOCUS       DATAIX010000603         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_285166, whole genome shotgun sequence.
ACCESSION   DATAIX010000603 DATAIX010000000
VERSION     DATAIX010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285166"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VHM65_08885"
     CDS             <1..403
                     /locus_tag="VHM65_08885"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HEX2705861.1"
                     /translation="PTAGSTARTTPETLLKAFWENHDPTQGNRQGNDVGTAYRSAIYW
                     TTDGQRAAAEATRAAFQPVLTQHGFGAITTEIRAAADAGPFYLAEDYHQQYLHKNPGG
                     YCNHGPTGMTCPVGLLSSDSTPAQQDIAPPG"
     gene            complement(424..>1535)
                     /locus_tag="VHM65_08890"
     CDS             complement(424..>1535)
                     /locus_tag="VHM65_08890"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HEX2705862.1"
                     /translation="VFLLFAMMVIVGILQKTGLFGFLAIWSAKRARGNPFRVMVLLCL
                     ITALASTVLNNVTTVMLVAPVTFLVCDRIGVRPEGYLIAEVMASNIGGTATLVGDPPN
                     IIIASRAGLTFNEFLAHLGPVIAIILVVFLVMVRFVFRKDFTADPDLVADVMSLRERD
                     AIEDPGLLVKGIAVLAVVLTGFVTGPFWGIEPSIVAILGAGALVALSRLAPSAYLAEV
                     EWETLAFFVGLFVMVGAMVKVGAIEGLAHWLAGVTGGQLGPSVGVLLVGSAVLSAIVD
                     NIPYVATMTPVVATLAGPGGPLAGHDVLWWALALGADLGGNATAIGASANVVVLGIAK
                     RNGYPISFWRFTSYGIGITAVTIAISGAYLWIRYF"
BASE COUNT          291 a          548 c          509 g          187 t
ORIGIN      
        1 gccaacagcc gggtcaacgg cccgcacgac acccgagacg ctgctcaagg cgttctggga
       61 gaaccacgac ccgacccagg gcaaccgtca gggcaacgac gtcggcacgg cctaccgctc
      121 ggcgatctac tggaccaccg acggccagcg cgcggcggcc gaggccaccc gcgccgcctt
      181 ccagcccgtc ctcacccagc acggcttcgg ggcgatcacg accgagatcc gggccgccgc
      241 cgacgccggc ccgttctacc tggccgagga ctaccaccag cagtacctcc acaagaaccc
      301 cggcggatac tgcaaccacg gcccgaccgg tatgacgtgc cccgtcggcc tgctgagctc
      361 ggacagcacc cccgcgcagc aggacatcgc cccgcccggg tgagccggtc gagggtctgc
      421 gcgtcagaag tagcggatcc acaggtaggc cccgctgatg gcgatggtca ccgccgtgat
      481 gccgatgcca tatgacgtga agcgccagaa gctgatcggg tagccgttgc gtttggcgat
      541 cccgaggacg acgacgttgg cgctggcccc gatggccgtg gcgttgccgc cgaggtcggc
      601 gcccagtgcc agggcccacc acagcacgtc atgcccggcc aagggacccc ccggaccggc
      661 cagtgtcgcc acgacgggtg tcatcgtcgc gacatacggg atgttgtcga cgatcgccga
      721 caggaccgcc gacccgacga ggagcacccc caccgacggc ccgagctgcc cgccggtgac
      781 ccccgccagc cagtgcgcca gcccttcgat cgccccgacc ttgaccatgg cgccgaccat
      841 gacgaacagg ccgacgaaga aggccagcgt ctcccactcg acctcggcga ggtatgccga
      901 cggcgcgagt cggctcaggg cgaccagggc gccggccccg aggatcgcga cgatgctcgg
      961 ctcgatgccc cagaacggtc ccgtcacgaa gcccgtgagg acgacggcca gcacggcgat
     1021 gcctttgacg agcaggcccg ggtcctcgat ggcgtcgcgc tcccgcagtg acatgacgtc
     1081 ggcgaccagg tccgggtcgg ccgtgaagtc cttccggaag acgaaccgca ccatcacgag
     1141 gaacaccacg agaatgatcg cgatcaccgg cccgaggtga gcgaggaact cgttgaaggt
     1201 caggcctgcc cggctggcga tgatgatgtt cggggggtcg ccgacgagcg tggcggtgcc
     1261 accgatgttg gaggccatga cctcggcgat gaggtagccc tccggccgca cgccgatccg
     1321 gtcgcacacg aggaaggtca ccggcgcgac cagcatgacc gtcgtgacgt tgttgagcac
     1381 cgtggacgcg agcgcggtga tgaggcacaa gagaaccatg acccggaagg ggttgccccg
     1441 ggcccgcttc gccgaccaga tcgcgagaaa cccgaacagc ccggtcttct gcaggattcc
     1501 gacgatgacc atcatggcga agagcaggaa aacga
//
LOCUS       DATAIX010000604         3935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_285337, whole genome shotgun sequence.
ACCESSION   DATAIX010000604 DATAIX010000000
VERSION     DATAIX010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285337"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VHM65_08895"
     CDS             complement(<1..646)
                     /locus_tag="VHM65_08895"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX2705863.1"
                     /translation="MMPALGTALGTNWMDPQYLLDNFGQQMFWVSLLIIFIECGLLFP
                     ILPGDSLLFAVGLFIASGSLKVNLGVALVLLVAAAFGGNVVGYEIGRAIGPPLYARDG
                     RFLRKKHVDQTTVFFDKHGNKALVLGRFVPIVRTFVTVVAGVGRMDRRRFFTWSFVGA
                     LLWAVGIALLGYYLGQVAVSKNNLEVAALLIVGVSVLPIVVEYWRHRRNAPSATD"
     gene            775..1659
                     /locus_tag="VHM65_08900"
     CDS             775..1659
                     /locus_tag="VHM65_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2705864.1"
                     /translation="MVRSRVRASLSALAALTTVGALAACGSSPTTPAATTGAAASSAA
                     DVCAKGSLAVATPGRLTIGTDKPAYEPWFKNDDPGNGEGYESAVAYAIAAKLGFAKGE
                     VAWVVAPFNAVVTPGKKKFDFDVNQVSISTERAKAVDFSSGYYDVRQAVVTYQGSPIA
                     GKTAVADLSGAKLGAQVGTTSYQAIVTQIKPSVAPSVYDSNDLAVQALKNKQIDGIVA
                     DLPTAFYMSAAQLDNGVIVGQLPASGATPERFGAVLSKGSPLTTCVTRAVDALRADGT
                     LARLVAAWLSSAGAPELT"
     gene            1664..2563
                     /locus_tag="VHM65_08905"
     CDS             1664..2563
                     /locus_tag="VHM65_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX2705865.1"
                     /translation="MSAPVGTPGPRRPGARRAGDGWVPSAVEQERRAYRRSRTLRSGF
                     TAATSTAVVIGLLGWVITSSSGWPRVRETFFSWDKAVDSFPAVIEGLWLNIRIMLVCG
                     VIIAALGLTIAILRTLRGPVFFPLRMVAAGFTDLFRGLPLLLLLLLLGFGVPALDIAG
                     LTSEVLVWGCVALILSYSAYVAEVFRAGIESVHPSQRAAARSLGLSHGQTLRFVVIPQ
                     AIRRVLPPLLNDLVSLQKDSGLISILGVIDAIRAAQIETASDFNFTPYVVAGLLFVTL
                     TIPMARFTDWVARRQGFQGGGVV"
     gene            2560..3294
                     /locus_tag="VHM65_08910"
     CDS             2560..3294
                     /locus_tag="VHM65_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX2705866.1"
                     /translation="MTLLAVSGLRKAFGANVVLDGFDLELDAGECVVLIGASGSGKST
                     VLRSINLLETVDDGVIHLAGDDITDPRVNADEVRARMGIVFQAYNLFPHLSVLANITL
                     APVRVHKLDRATARRQAMAMLERVGLADKAAARPDDLSGGQQQRVAIARALVSSPELM
                     LLDEITSALDPELVGEVLELLVTLKGDGMTMLLASHEMAFAKAVADRVAFLHGGRVHE
                     SGPPAELLVNPREERTRQFLSRFVGL"
     gene            3300..>3935
                     /locus_tag="VHM65_08915"
     CDS             3300..>3935
                     /locus_tag="VHM65_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX2705867.1"
                     /translation="MAEPLGVGPWEGPWPDDPRLDPELLRDGDRRNVADRYRYWGLEA
                     IVADLDTTRHPFHVAVENWGHDFNIGSVIRTANAFNANAFHIVGKRRWNRRGAMVTDR
                     YQHEQHHPTVAHLVTWAATAATGRTGGGEARAIPLVGIDNLPGSRPLETADLPRECVL
                     VFGQEGPGLSDDMRDACDVVLHISQFGSTRSINAGAAAAIAMHAWVRQHVFG"
BASE COUNT          583 a         1439 c         1314 g          599 t
ORIGIN      
        1 ggtctgtcgc gctcggggcg ttgcgtcggt ggcgccagta ctcgaccacg atcggcagca
       61 ccgagacgcc gacgatgagg agcgcagcga cttcgaggtt gttcttgctg accgcgacct
      121 ggccgaggta gtagccgagc agcgcgatcc ccacggccca gagcagcgcc ccgacgaagc
      181 tccaggtgaa gaaccgccgc cggtccatgc ggcccacccc ggcgacgacc gtgacgaacg
      241 tccgcacgat cgggacgaac cggcccagca cgagcgcctt gttgccgtgc ttgtcgaaga
      301 acaccgtcgt ctggtcgacg tgcttcttcc gcaggaagcg cccgtcgcgg gcgtagagcg
      361 gcgggccgat cgcccggccg atctcgtaac cgacgacgtt gcccccgaac gccgccgcga
      421 cgagcagcac cagcgccacg cccaagttga ccttgagcga cccgctcgcg atgaacagcc
      481 cgacggcgaa cagcagcgag tcacccggca ggatcgggaa gagcagcccg cactcgatga
      541 agatgatgag caggctcacc cagaacatct gctggccgaa gttgtcgagc aagtactgcg
      601 ggtccatcca gttggttccg agggcggtcc cgagcgcagg catcatggtc accaagggta
      661 tgtcgtcacc cacgtcgagg ccgccatcct gcggcgcgtt gggagcggct acgatcaggc
      721 ccgacccagg cggccgccaa ctggttgctg cgcccgaccc gcaaggaagt cccgatggtt
      781 cgctcgcgcg tccgcgcctc gctctcagcc ctcgctgctc tcaccacggt cggggcgctc
      841 gcggcctgcg gctccagccc cacgaccccc gcggcgacca cgggcgcggc cgcgagctcc
      901 gccgccgacg tgtgtgccaa ggggagcctg gctgtcgcca cgcccggccg gctcaccatc
      961 gggaccgaca agccggccta cgagccgtgg ttcaagaacg acgacccggg caacggcgag
     1021 gggtacgagt cggccgtcgc ctacgccatc gcggcgaagc tgggcttcgc caagggcgag
     1081 gtcgcctggg tggtcgcccc gttcaacgcg gtcgtcaccc cgggcaagaa gaagttcgac
     1141 ttcgacgtca accaggtctc gatcagcacg gagcgcgcga aggcggtcga cttctcctcc
     1201 ggctactacg acgtgcgcca ggccgtcgtc acgtaccagg ggtcgcccat cgcgggcaag
     1261 accgccgtgg cggatctctc cggcgccaag ctcggcgccc aggtgggcac gacgtcatac
     1321 caggcgatcg tcacgcagat caagccctcg gtcgcgccgt cggtctacga cagcaacgac
     1381 ctcgcggtcc aggcgctgaa gaacaagcag atcgacggga tcgtcgccga cctgccgacg
     1441 gcgttctaca tgtcggccgc gcagctcgac aacggggtca tcgtcgggca gctgccggcc
     1501 tcgggcgcca ccccggagcg cttcggtgcc gtcctgtcca agggttcgcc cctgaccact
     1561 tgcgtcacca gggcggtcga cgcgctccgg gccgacggca cgctggccag gctcgtcgcg
     1621 gcgtggctgt ccagcgccgg cgctcccgag ctcacgtagc cagatgagcg ctcccgtcgg
     1681 cactcccggc ccgcgaaggc cgggagcgcg gcgtgctggg gacggatggg tcccctcggc
     1741 ggtcgagcag gaacgccgtg cctaccgccg gtcgcggacg ctgcgctccg ggttcaccgc
     1801 ggcaacgagc acggcggtcg tcatcggcct gctcggctgg gtcatcacgt cgtcgtcggg
     1861 atggcctcgc gtcagggaga ccttcttcag ctgggacaag gccgtcgact ccttcccggc
     1921 cgtcatcgag gggctctggc tcaacatccg gatcatgctc gtgtgcggcg tcatcatcgc
     1981 ggccctcggc ctcaccatcg cgatcctgcg gacgctgcgc gggccggtgt tcttcccgct
     2041 gcggatggtc gcggccgggt tcaccgacct gttccgcggg ctgcccctgc tcctcctgct
     2101 gctcctgctc ggcttcggcg tcccggccct cgacatcgcg ggcctgacga gcgaggtgct
     2161 cgtgtggggc tgtgtcgcgc tcatcctgtc ctactcggcc tacgtcgccg aggtgttccg
     2221 ggccgggatc gagtcggtcc acccgtcaca gcgcgcggct gcccgctcgc tcgggctcag
     2281 ccacggccag accctgcgct tcgtcgtcat cccccaagcc atccgccggg tgctgccgcc
     2341 cctgctcaac gacctcgtgt ccctgcagaa ggactccggg ctcatctcga tcctcggtgt
     2401 catcgacgcg attcgcgcgg cccagatcga gacggccagc gacttcaact tcacgccgta
     2461 tgtcgtcgcc gggctcctct tcgtcacctt gacgatcccg atggcgcggt tcaccgactg
     2521 ggtcgcgcgg cgccagggct tccagggcgg cggtgtcgta tgaccctgct cgccgtcagt
     2581 gggctgcgca aggccttcgg tgcgaacgtc gtcctcgacg gcttcgacct cgagctcgat
     2641 gcaggggagt gcgtcgtgct catcggcgcc tccgggtcgg gcaagtcgac cgtcctgcgc
     2701 agcatcaacc tcctcgagac cgtcgacgac ggggtgatcc acctcgctgg ggacgacatc
     2761 accgacccgc gggtgaacgc cgacgaggtc cgggcgcgga tggggatcgt cttccaggcc
     2821 tacaacctct tcccccatct cagcgtcctg gcgaacatca ccctggctcc ggtgcgggtg
     2881 cacaagctgg accgggcgac cgcacggcgc caggcgatgg ccatgctcga gcgggtcggg
     2941 ctcgccgaca aggccgccgc tcgccccgac gacctctcgg gagggcagca gcagcgggtc
     3001 gccatcgccc gggccctggt cagctcaccc gagctcatgc tcctcgacga gatcacctcg
     3061 gccctggacc cggagctcgt cggggaggtg ctcgagctgc tcgtcaccct caagggcgac
     3121 gggatgacga tgctgctcgc cagccacgag atggccttcg ccaaggcggt agccgaccgg
     3181 gtcgccttcc tgcacggcgg ccgggtccat gagtccggac cgcccgccga gctgctcgtc
     3241 aacccgcggg aggagcggac ccgtcagttc ctctcccgtt tcgtgggcct gtgaccgaca
     3301 tggccgagcc gctcggtgtg ggcccgtggg agggtccgtg gcccgacgac ccgcgcctgg
     3361 acccggagct gctccgcgac ggcgaccggc gcaatgtggc cgaccgctac cgctactggg
     3421 ggctcgaggc gatcgtggcc gacctcgaca cgacccggca cccgttccac gtggccgtcg
     3481 agaactgggg gcacgacttc aacatcggct ccgtcatcag aaccgcaaac gccttcaacg
     3541 ccaacgcttt tcacatcgtc ggcaagcgcc ggtggaaccg caggggcgcg atggtcaccg
     3601 accgctacca gcacgagcag catcacccga cggtggccca cctcgtcacc tgggcggcca
     3661 ccgcggccac cggccgcacg ggcggtggtg aggcgcgcgc cataccgctg gtggggatcg
     3721 acaacctccc cggctcacgg ccgctcgaga ccgctgatct gccccgggaa tgtgtcctcg
     3781 tcttcggcca ggaagggccc gggctcagcg acgacatgcg cgacgcgtgc gacgtggtcc
     3841 tgcacatcag ccagttcggc tcgactcgct cgatcaacgc cggtgctgcc gcggccatcg
     3901 cgatgcatgc gtgggtccgc cagcatgtct ttggc
//
LOCUS       DATAIX010000605         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_195739, whole genome shotgun sequence.
ACCESSION   DATAIX010000605 DATAIX010000000
VERSION     DATAIX010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195739"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VHM65_08920"
     CDS             complement(<1..711)
                     /locus_tag="VHM65_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705868.1"
                     /translation="MRMRPDEVTSPSAQDSLVRSAAEVAGGPQGRYAAPRSWRRVGAV
                     LVGLSSVPLALGVLERGHCISAGWNTPDQFWHACFSDLTTTYAGTGLSSGPSAYLFGM
                     ADAATPSQPPLTGLIMTGVAALVPDAPLDERMRWYFALWAVLAAVLVSVVVWMTTASV
                     PRHPVRAAQVALSPLVALTLFVAPDVVGVALVSGALLAWGRRRPPLAGVLLGLAISAR
                     TYPLVILAALLFVCLRGGR"
     gene            complement(723..>2491)
                     /locus_tag="VHM65_08925"
     CDS             complement(723..>2491)
                     /locus_tag="VHM65_08925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX2705869.1"
                     /translation="GVSPSGIARAVIAGIKGGAPTQGGSTITQQYVKNYFLTQDRTVS
                     RKFNEIIISLKIDGQETKAKILENYLNTIYYGRGAYGVQTASRAYFNKDVSRLTVAEG
                     AVLASVIRAPSLYDPALGAEQLANLKDRFTYVLDGMVGQGWLSSAERAAATFPKILPA
                     KRAQANGGTTGYIIADVKRELEAKLKLTQEDIERGGLRVVTTIDRKSQAAAVAAVEKT
                     LPTDKSMKDVYAGLVAIRPGDGAIVAEYGGKDFITRETSSATGAILQGGSTFKPFALI
                     AALQQGLSTKSRVDGSSPYRLADGKSLKNAGNASYGMVDLRYATGHSINTAFLRLNEK
                     VGPDKTRAAAIAAGVPESTPGLGTNLTNVLGTASPHVVDMANAFATIAAQGRRATPYL
                     IRSVTSAPLELKYTAKPVLAAAFSKDVAADVIDAMTQPTKSGGTASRARELGRPVAGK
                     TGTTDSEKSVWFTGYVPQLSVSVGMFKDVNGTAAPLHNVGGYSVLYGGTYPLSIWLAF
                     MDKVMDGVPEVDFPERAGIGDDQVKPVYTPRPSTSSTTAPPTTPPVPSSTTTTAPTPS
                     STSTTLPAPGARAGSRGRFGPS"
BASE COUNT          367 a          851 c          891 g          382 t
ORIGIN      
        1 ccgcccgccg cgcagacaga cgaacagcag tgccgcaagg atcacgagcg ggtaggtccg
       61 cgccgagatc gccagtccga gcagcacccc ggccaaggga ggtcggcggc gtccccacgc
      121 cagcagcgct cccgaaacaa gggccacccc gacgacgtcc ggcgcgacga agagcgtgag
      181 agcgacgagg ggggacaacg cgacctgagc cgcccgaacc gggtgccgtg gcaccgacgc
      241 ggtcgtcatc cacacgacga cggagacgag cacggccgcg agcaccgccc agagggcgaa
      301 gtaccacctc attcgctcgt cgagcggcgc gtccgggacc agggccgcca cgcccgtcat
      361 gatcagcccg gtcagcggcg gctgtgacgg ggtcgccgca tcggccattc cgaagaggta
      421 ggccgacggg ccgctcgaca agccggtgcc ggcatacgtc gtcgtgaggt cggagaagca
      481 cgcgtgccag aactggtccg gggtgttcca gccggccgag atgcagtggc cgcgctcgag
      541 cacgcccagg gccagcggga ccgagctgag cccgacgagg acggcaccga cccggcgcca
      601 cgagcggggg gcggcatacc ggccctgcgg tccgccggcc acctcggcgg ccgacctgac
      661 gagcgaatcc tgagcggacg gcgaggtgac ctcgtcggga cgcatcctca tgggcgccgg
      721 gtctacgaag gcccgaagcg cccgcgggaa cccgcgcgag ctcctggtgc cgggagagtg
      781 gtcgatgtcg acgacggggt cggcgctgtc gtcgtggtgc tcgacggcac cggcggtgtc
      841 gtgggcgggg ccgtcgtcga cgaggtcgag ggccgaggcg tgtagacggg cttgacctgg
      901 tcgtcgccga tcccggcccg ctccgggaag tcgacctccg gcactccgtc catgaccttg
      961 tccatgaacg ccagccagat cgacagcggg taggtccccc cgtagagcac cgagtagccc
     1021 ccgacgttgt gcagcggcgc cgcggtgccg ttgacgtcct tgaacatccc gaccgacacg
     1081 gacagctgcg ggacgtaccc ggtgaaccac accgacttct cgctgtcggt cgtccccgtc
     1141 tttcccgcga ccggacgccc gagctccctc gcccgcgacg ccgtgccgcc ggacttggtc
     1201 ggctgggtca tcgcgtcgat gacgtccgcg gccacgtcct tgctgaacgc ggcggcgagc
     1261 accggcttcg cggtgtactt gagctcgagg ggggccgagg tcaccgacct gatgaggtat
     1321 ggcgtcgcgc gcctgccctg ggcggcgatg gtcgcgaagg cgttggccat gtcgacgacg
     1381 tggggcgagg ccgtgcccag cacgttggtg aggttcgtgc cgagaccggg cgtcgactcc
     1441 ggcacccccg cggcgatcgc ggcggcgcgc gtcttgtccg gcccgacctt ctcgttgagg
     1501 cgcaggaacg cggtgttgat cgagtgcccc gtcgcgtagc gcaggtcgac catcccgtat
     1561 gacgcgttgc cggcgttctt gaggctcttg ccgtcggcga ggcggtaggg gctgctgccg
     1621 tcgacccgcg acttggtgct gaggccctgc tgcagagccg cgatgagtgc gaacggcttg
     1681 aaggtcgaac cgccctgcag gatggccccg gtggccgagc tcgtctcgcg cgtgatgaag
     1741 tccttgccgc cgtactcggc gacgatcgca ccgtctccgg ggcggatcgc gacgagcccg
     1801 gcatacacgt ccttcatgct cttgtccgtc ggcagggtct tctcgaccgc ggcaaccgcc
     1861 gcggcctggg acttcctgtc gatcgtcgtg acaacccgca ggccaccgcg ctcgatgtcc
     1921 tcctgagtca gcttgagctt ggcctcgagc tcgcgcttga cgtcggcgat gatatagccg
     1981 gtcgtgccac cgttggcctg cgccctcttg gcaggcaaga tcttcgggaa cgtcgccgcc
     2041 gcccgctcgg ccgagctgag ccagccctgg ccgaccatcc cgtcgaggac gtaggtgaag
     2101 cggtccttga ggttggccag ctgctcggcg ccgagcgccg ggtcgtagag gctcggggcc
     2161 cggatcaccg aggccagcac ggcgccctcg gcgacggtga gcctgctcac gtccttgttg
     2221 aagtacgcgc gcgaggccgt ctggacgcca tacgcgcctc ggccgtagta gatcgtgttg
     2281 aggtagttct ccaggatctt ggccttggtc tcctggccgt cgatcttgag cgagatgatg
     2341 atctcgttga acttgcggct gacggtgcgg tcctgggtga ggaagtagtt cttgacgtac
     2401 tgctgggtga tcgtcgaccc gccctgcgtc ggagccccac ccttgatccc cgcgatgacg
     2461 gcccgggcga tgccggacgg ggacacaccc t
//
LOCUS       DATAIX010000606         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_135202, whole genome shotgun sequence.
ACCESSION   DATAIX010000606 DATAIX010000000
VERSION     DATAIX010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135202"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VHM65_08930"
     CDS             <1..323
                     /locus_tag="VHM65_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705870.1"
                     /translation="PDTRGTWECVPDITAVGSEDFGYRQPCSPPQFFHADVDNPDPFR
                     FGPPVPETQWYDETHDLLVHYLPRTTPPPPDPFGSDPCRENPHHDLEADPSWRERLHA
                     GGDH"
     gene            323..>1537
                     /locus_tag="VHM65_08935"
     CDS             323..>1537
                     /locus_tag="VHM65_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705871.1"
                     /translation="MGEDQDLPRRPKHPELSEDEDKPQRGDSPPGSVGHTDPPKETDP
                     DAAWVPDPGRADEWRGIANEIGREHRPRREDVYPYLLIRAMSPGDRGQWPLWPPIVCW
                     ESPDILLIDAAWSGDFHPSRLVASPTAGRSYRVFVRIWNLGLLPAIGVHVRAWYVNPG
                     FFGPGNQGNPYYQPSPIGGQMAHLEDRTRPGATRLVELDHAWDIPATLTGHECLIASV
                     SCPADQWSGSMSVNADRHIGQRNLTILEPASSAKDMFATLGALIEKGATLELTHGGPA
                     VLPLLHAVAGGRLRDGERAGKVLAPELADLRAGVGIGVSQHLLTAFRSEGGSVVVDSA
                     RLAALAGELGRLTNDAGHDDRDERDQRRARAARVHPFAVAGGTRRLIDRLDPERLATV
                     AWTSPQELASAFV"
BASE COUNT          225 a          591 c          526 g          195 t
ORIGIN      
        1 ctccagacac gcgcggcacg tgggagtgcg tcccggacat cacggcggtc ggcagcgagg
       61 acttcggcta ccgccagccg tgcagtccgc cgcagttctt ccacgccgac gtcgacaacc
      121 cggacccgtt ccggttcggc ccgccggtcc cggaaaccca gtggtatgac gagactcacg
      181 acctgctcgt ccactacctg ccgcggacga cgccaccgcc accggacccc ttcgggtccg
      241 acccgtgccg ggagaacccg caccacgacc tcgaggccga ccccagctgg cgcgagcggc
      301 tgcacgccgg aggagaccac tgatgggcga ggaccaggac ctgccgcggc gcccgaagca
      361 ccccgagctg tccgaggacg aggacaagcc ccagcgcggc gactctccac cgggctcggt
      421 gggtcacacc gaccctccca aggagaccga ccccgacgcc gcctgggtgc cggaccccgg
      481 gcgggcggac gagtggcgcg gcatcgccaa cgagatcggc agggagcatc ggccccgccg
      541 cgaggacgtc tacccctacc tgctcatccg ggcgatgtcg cccggagacc gggggcagtg
      601 gccgttgtgg cccccgatcg tctgctggga gtcgccggac atcctgctca tcgatgcggc
      661 gtggtcgggt gacttccacc cctcacggct ggtggcgtcc ccgacggccg gccggtccta
      721 ccgggtcttc gtccgcatct ggaacctcgg cctgctaccg gccatcggcg tccacgtccg
      781 cgcctggtac gtcaacccgg gcttcttcgg gccgggcaac cagggcaacc cgtactacca
      841 gccatctccc atcggcggtc agatggcgca cctcgaggac cggacccggc ccggggcgac
      901 gaggctcgtc gagctcgacc acgcctggga catcccggcc acgctcaccg gtcacgagtg
      961 cctcatcgcg tcggtgtcct gccccgctga ccagtggagc gggagtatgt cggtcaacgc
     1021 cgaccgccac atcggccagc gcaacctgac gatcctcgag ccggcctcct cggcgaagga
     1081 catgttcgcc accctgggcg ccctcatcga gaagggcgcg acactcgagc tcacccacgg
     1141 cgggccggcc gtcctgccgc tgctgcacgc cgttgccggc gggcggctgc gcgatggcga
     1201 acgcgccggc aaggtcctgg ctcccgagct tgcggacctg cgggcgggcg tcggcatcgg
     1261 cgtgagccag cacctgctga ccgccttccg gtccgagggt ggcagcgtcg tcgtcgacag
     1321 cgcccggctc gccgccctcg cgggggagct cggccgactc acgaacgacg cggggcacga
     1381 cgaccgcgac gagcgtgacc agcgccgggc gagggcggcc cgggtgcacc ccttcgcggt
     1441 ggccgggggg acccgccggc tcatcgaccg gctcgatccg gaacggcttg ccacggtggc
     1501 gtggacgagc ccccaagagc tggcaagcgc cttcgtc
//
LOCUS       DATAIX010000607         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_225480, whole genome shotgun sequence.
ACCESSION   DATAIX010000607 DATAIX010000000
VERSION     DATAIX010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225480"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="VHM65_08940"
     CDS             <1..857
                     /locus_tag="VHM65_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705872.1"
                     /translation="LRLDDIDEDEEPVAPPALLPGGPPWLDPPAPSGGVWHDVPSQAP
                     VAPAPSSAATTPATASRPAVQALSLSLEAARAEVAQLQAELEATTASLVPTQIQAEQL
                     ARDVQSLSAARHELLAKIEAQRTQLRLRTVRAGKVRRTEADQAGSAIAPFTDPVRQLE
                     HEIYLEWVTRIPAAEKESRPLVTCKLGPEFLASMAQVHGVSRRKVVQVVVEVLTGLAE
                     QLDSRELHLLRTGPGGDDPPVVRGDGARCWRVAVQRGTPAAARLHFWRTASGVELSRV
                     VVHDDLRP"
     gene            1270..1461
                     /locus_tag="VHM65_08945"
     CDS             1270..1461
                     /locus_tag="VHM65_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705873.1"
                     /translation="MAVLGSAPQFDHLYKWYFTPEVGAAMRWAASVNLDTDVEHEASV
                     RAIDGACGIGPPPGYVDFP"
BASE COUNT          226 a          583 c          564 g          201 t
ORIGIN      
        1 ccctgcgcct cgacgacatc gacgaggacg aggagcccgt cgcccccccg gcgttgctcc
       61 ccggcggccc gccttggctg gacccgccag cacccagcgg gggggtgtgg cacgacgtcc
      121 cctcccaggc gccggtggcg cctgcgccct cgtctgcggc aaccaccccg gccactgcct
      181 ctcgaccggc cgtccaggcc ctgagcctct ccctcgaggc ggcccgcgcg gaagtggccc
      241 agttgcaggc cgagctcgag gcgaccacgg cgagcctggt accgacgcag atccaggccg
      301 agcagctcgc gcgcgacgtg cagtccttga gtgcggcacg ccatgagctc ctcgccaaga
      361 tcgaggccca acgcacccag ctccggctgc gcaccgtccg cgcgggcaag gtgcgtcgca
      421 ccgaggcaga tcaagccggc tcggccatcg cgcccttcac cgacccggtg cgccagctcg
      481 agcacgagat ctacctcgag tgggtcaccc ggatacccgc agccgagaag gagagccggc
      541 cgttggtcac gtgcaaactc ggcccggagt tcctggcgag catggcgcag gttcacggcg
      601 tgagccggcg caaggtggtc caggtcgtcg tcgaggtgct caccggcctc gccgagcagc
      661 tcgacagcag ggagcttcac ctcctgcgca cggggccagg cggcgacgac ccgcccgtgg
      721 tccgtggcga cggtgctcgg tgctggcgcg tggccgtcca gcgcggcacg ccggccgcag
      781 cgcgcctgca cttctggcga acggcgagtg gcgtcgagct ctcccgggtc gtggtgcacg
      841 acgacctgcg accctgagcg ctcaaccccg ggcctgaagc gcgctccgtg ccaaggcgat
      901 cccgctggcc gtcggctctc gggtccagcc gaaccggtca ccgcgaggga ccgccgagcc
      961 gacgcagcac gcaaacggtc gactcggcgt cgttcatcac gttgccgcgc agggggtggc
     1021 cgtggcgacg ttgccagccg atggcggcca tgagggcacc cacgtcgatc ggtcccgatc
     1081 ggcacctctc tgcccgcccc ggctcgcggc ggcagcaagc agcccggcgt cgcgccacag
     1141 cgccgagcgt gaggcaggcg tgcgggaggg cgagatgcca gccgccggcg gcgtgcgggg
     1201 caaaccaggc cggggcgcga cggcgtcctc ggccatcgtg ggtcccacaa ggacatcggg
     1261 ccggagtggg tggcggttct ggggtcggcc ccgcagttcg accacctcta caagtggtac
     1321 ttcaccccgg aggtcggcgc ggcgatgcgc tgggccgcca gcgtgaacct cgacacggat
     1381 gtcgagcacg aggccagcgt gcgggcgatc gacggtgcgt gcggtatagg tccacctccc
     1441 ggctacgtgg acttcccgta gacaacaccc gtgcgggccg acggccgggt ccgctgcggc
     1501 ggctgccaga tcaacgtgag gcctcgcaag aacctgatcc tggtcgagga cgccgtcggc
     1561 ggcctgccga tcgc
//
LOCUS       DATAIX010000608         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_196049, whole genome shotgun sequence.
ACCESSION   DATAIX010000608 DATAIX010000000
VERSION     DATAIX010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196049"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VHM65_08950"
     CDS             <1..482
                     /locus_tag="VHM65_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705874.1"
                     /translation="AMLIAAESLGPIQSNTPLSPELALGPGLRHESHVVIDFGDDGMT
                     QGRAHPMIDPLLRLERIATEAADPTCGVLLLDLVLGFGSHPDPAPDLAAAIRRAREVA
                     AAAGRELPVVVSLIGVEADPQGLTACATALNAAGASVFLSNAAATRHAVSLCAEGVS"
     gene            482..>1520
                     /locus_tag="VHM65_08955"
     CDS             482..>1520
                     /locus_tag="VHM65_08955"
                     /inference="COORDINATES: protein motif:HMM:NF018275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HEX2705875.1"
                     /translation="MSSPTSALRGLLGTDPSVATAGVSLFAEALRGQAVRVTEVDWRP
                     PMEGTDTDLARVMADPRREGANAEAFARMTSAGAELVDVRPASEVLGLTPGTFLHAGP
                     PLDWERASGPMRGGLIGAMLLEGLAATPEAAEAALARGDGITLEPCHHLGAVGPMAGI
                     VSPSMWMFVLRDEAHDGTAYCTLNEGLGKVLRYGAYGPEVIERLRWMSAVLGPLLQAA
                     VRTCGPVDIRAIIAQMLQMGDEGHNRNRAGTLMFLRDILPSLITVDAPSSDIAEAVRF
                     AGANDHFFLNLDMPACKLATAAAHGIPGSTVVTTMARNGTDFGIRVSGAGDGWFTGPA
                     LTPQGLFLGAFG"
BASE COUNT          185 a          591 c          521 g          223 t
ORIGIN      
        1 aggcgatgct catcgccgcc gagtccctcg gcccgatcca gtcgaacacc cccctctccc
       61 ccgagctcgc gctcgggccg ggcctgcgcc atgagagcca cgtcgtcatc gacttcggcg
      121 acgacggcat gacccagggg cgcgcgcatc cgatgatcga cccgctcctg cgcctggaac
      181 ggatcgccac cgaggccgcc gacccgacgt gtggcgtgct gctcctcgac ctcgtcctcg
      241 gcttcgggtc ccatcccgac cccgctccgg acctcgcggc ggcgatccgc cgggcccggg
      301 aggtcgcggc cgccgccgga cgggagctgc cggtcgtcgt ctcgctcatc ggcgtcgagg
      361 ccgacccgca ggggctgacc gcctgcgcca ccgccctcaa cgccgcgggg gcgtcggtct
      421 tcctgtccaa cgccgccgcc acgcggcacg ctgtctcgct gtgcgccgaa ggggtctcgt
      481 gatgagctca ccgacgtccg cactgcgagg gctgctcggc acggacccgt cggtcgcgac
      541 ggccggggtg agcctgttcg ccgaggcgct gcgcggccag gccgtgcggg tcaccgaggt
      601 cgactggcgc cccccgatgg agggcaccga caccgacctg gccagagtca tggccgaccc
      661 gcgccgggag ggggccaacg ccgaggcctt cgcccggatg acctccgctg gtgccgagct
      721 cgtcgacgtc cgcccggcct cggaggtgct cggtctcacc cccggcacct tcctgcatgc
      781 cggtccgccc ctggactggg agcgcgcctc cgggccgatg cgcggcgggc tcatcggcgc
      841 catgctcctc gagggcctgg ccgcgacgcc cgaggcggcc gaggcggcgc tcgcccgcgg
      901 cgacggcatc accctggaac cgtgccacca tctcggcgcg gtcggcccga tggccggtat
      961 cgtctcgccc tcgatgtgga tgttcgtcct gcgcgacgag gcccatgacg gcacggccta
     1021 ctgcaccctc aacgagggct tgggcaaggt cctgcggtat ggcgcgtacg gtccggaggt
     1081 catcgagcgc ctccgctgga tgtcggccgt cctgggcccg ctgctccagg cggccgtccg
     1141 cacgtgcggg ccggtcgaca tcagggcgat catcgcccag atgctgcaga tgggcgacga
     1201 gggccataac cgcaaccgag ccggcacctt gatgttcctg cgcgacatcc tgccctcgct
     1261 catcaccgtg gacgcgccgt cgtcggacat cgccgaggcg gtccgtttcg ccggcgccaa
     1321 cgaccacttc ttcctcaacc tcgacatgcc cgcctgcaag ctcgcgaccg ccgccgctca
     1381 cgggatcccc ggttcgacgg tcgtcacgac gatggcccgc aacgggacgg acttcgggat
     1441 ccgggtctcc ggcgcggggg acgggtggtt caccggcccg gcgctgacac cccaggggtt
     1501 gttcctcggt gccttcggtc
//
LOCUS       DATAIX010000609         2689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_225630, whole genome shotgun sequence.
ACCESSION   DATAIX010000609 DATAIX010000000
VERSION     DATAIX010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225630"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VHM65_08960"
     CDS             complement(<1..803)
                     /locus_tag="VHM65_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX2705876.1"
                     /translation="MSDAERIEQIRALELCKSALAAAQARVAVDFDTSRRAQEADRGV
                     AAAGRGRGVGAEIALARRESPTRGSRHLGLARALVLDMPATMAALEAGQASEWTATLV
                     CQATAILTAEDRRAVDAELANDLPSMSDRQAASAARAAAYRIDPTSVVRRVRGAESDR
                     CVSMRPAPDCMARLSALLPVKDGVAVYAALRACADTARAGGDSRTQGQVMADTLTTRT
                     TGYASPDEIPVQVNLVMSDTTLLAGGDDPGRFQGYGPVPAPLARHLATAP"
     gene            1071..1238
                     /locus_tag="VHM65_08965"
     CDS             1071..1238
                     /locus_tag="VHM65_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705877.1"
                     /translation="MTLNHTIRPLYRHGETGTVVGWAGQRVIVALDHRTGRFATGQIR
                     CPPVGLDLLPT"
     gene            complement(1289..2101)
                     /locus_tag="VHM65_08970"
     CDS             complement(1289..2101)
                     /locus_tag="VHM65_08970"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX2705878.1"
                     /translation="MTRTRRLRLLARVCHPAPTAAVTTLACLLALSRGLAPSRVGVVT
                     LAVLTGQLTIGWSNDLVDADRDRQSGRADKPLAQGELPVALVYRAIGAALVVCVAASF
                     WDGWSAGLVHLGLVVSSGWVYNLWLKRTAASFLPYAVAFGALPAFVTLSGSPAVVPPW
                     WLAATGALLGVGAHFVNVLPDLADDAATGVQGLPHRMGERGARSAAAVALLGGSAVSV
                     LGPIGRVPGWAYAGLGLAAALAIVTVTGRGKAPFRAAIGIALVDVVVLVLRS"
     gene            2178..>2689
                     /locus_tag="VHM65_08975"
     CDS             2178..>2689
                     /locus_tag="VHM65_08975"
                     /inference="COORDINATES: protein motif:HMM:NF019988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705879.1"
                     /translation="MSQLRAVRGVVPPHRYAQEELTEAFAGVVGPRGVSRPLLRRFHS
                     NAGVSFRHLALPIERYAGLKDFGEANDEFIGAGVDLGAEAVTTALEAAGLTPGDVDLV
                     VSTTITGRAVPSLEARVAGRIGLRTDVKRVPLVGLGCVAGAAGIARAHDYLLGHPAEV
                     AVLMSVELCSL"
BASE COUNT          399 a         1000 c          957 g          333 t
ORIGIN      
        1 ggggcggtgg cgaggtgccg ggccaacggg gccgggactg gcccatagcc ctggaaccgg
       61 cccgggtcgt cgccgccggc gagcagcgtg gtgtcactca tgacgaggtt gacctggacg
      121 gggatctcgt ccgggctggc atagccggtc gtccgcgtgg tcagcgtgtc ggccatgacc
      181 tgcccctggg tgcgactgtc gccgccggcg cgggccgtgt cggcgcaggc ccgcagcgcg
      241 gcatacacag cgaccccgtc cttgaccgga agcagggcgc tgagccgggc catgcagtcg
      301 ggggccggcc gcatcgagac gcagcggtca ctctcggcgc cccggacccg gcgcaccacg
      361 ctcgtggggt cgatccggta ggccgccgcg cgggccgccg acgccgcctg gcggtcactc
      421 atcgagggca ggtcgtttgc gagctcggca tcgaccgcgc ggcggtcctc ggcggtcaga
      481 atcgccgtcg cttggcagac cagggtcgcg gtccactcgc tcgcctgccc agcctcgagc
      541 gcagccatcg tggccggcat gtccagcacc agagccctgg ccaagcccag atggcgcgac
      601 ccccgggtcg gggactcgcg gcgcgcgagg gcgatctcgg cgccaacccc gcgcccgcgc
      661 cccgctgcgg cgacgccccg gtcagcctcc tgcgcacgcc gggaggtgtc gaagtccacg
      721 gcaacccgtg cctgcgcggc cgcgagcgcg ctcttgcaca gctccagggc ccggatctgc
      781 tcgatccgct cggcgtcgct cacctgtcgg tccagcccac ccagactcac ccggaacgca
      841 tccaccacgg cccgcggcaa cgggccgagg ctggccccag cgggggccgg aggcggggtg
      901 gacgtcatac tcgattatcg cacaccagtt cgattttggg aagaggcgag cgcactgcaa
      961 actcagcgaa atcccgtgtc ggacataact attcacctgc ctgacggcga gtccggtcga
     1021 cccgaccggg tgactgtgat gccctgggca ccatcgacgg cggggaccgg gtgacgctca
     1081 accacaccat ccggcccctc taccggcacg gcgaaaccgg gacagtcgtg ggctgggcag
     1141 gtcaacgcgt catcgtcgcg ctcgaccacc gcaccggacg cttcgccacg ggccagatcc
     1201 gctgcccacc agtcggactc gatctgttgc cgacctgagc gcgctggcgc gacccaggaa
     1261 gtccagcggg acgtcgagcc aatcatcgtc agctgcgcag gacgaggacc acgacatcga
     1321 cgagggcgat cccgatggcc gcgcggaacg gcgccttgcc ccggccggtc accgtgacga
     1381 tggcgagcgc cgctgccagc ccgaggccgg catacgccca tcccggcacg cggccgatcg
     1441 ggccgagcac gctgaccgcg gacccgccga gcagcgcgac ggccgccgcc gagcgcgccc
     1501 cgcgctcgcc catccggtgc ggcagtccct gtacgccggt cgcggcatcg tcggccaggt
     1561 cgggcagcac gttgacgaag tgcgcaccca ccccgagcag cgccccggtc gcggccagcc
     1621 accagggcgg gaccactgcc ggactgcccg acagcgtcac gaacgccggc agcgcgccga
     1681 acgcgacggc atacgggagg aacgacgccg cagtccgctt gagccacagg ttgtagaccc
     1741 agcccgacga gacgacgagt cccaggtgca ccagccccgc agaccagccg tcccagaacg
     1801 acgcagccac acagaccacc agggccgcgc caatcgcccg gtacacgaga gcaaccggaa
     1861 gctccccctg tgccaggggt ttgtcggcgc ggcccgattg ccggtcgcgg tcggcgtcga
     1921 cgaggtcgtt ggaccagccg atcgtcagct ggccggtgag aaccgcgagc gtgacgacac
     1981 cgacccggct cggcgcgagg ccccgggaca gcgccagcag gcacgcgaga gtcgtcaccg
     2041 ccgcggtcgg cgccgggtgg cagacgcggg ccagcagtcg caggcggcgt gtccgggtca
     2101 ccccctgagg gtaggccggc cagcgacgga cccgggccgt gggcgggacg gccacccggc
     2161 tcgtctactc ttcgcagatg agtcagctgc gcgccgtccg aggggtcgtg ccaccccatc
     2221 gctatgcgca ggaggagctc accgaggcgt tcgcgggcgt ggttggcccg cggggggtca
     2281 gccggccgct gcttcgccgg ttccactcca acgcgggcgt cagcttccgt cacctcgccc
     2341 tgccgatcga gcggtatgcc gggctcaagg acttcggtga ggcgaacgac gagttcatcg
     2401 gggcgggcgt cgacctcggt gccgaggcgg tcacgacggc actcgaggca gccggcctga
     2461 cgcccgggga tgtcgatctt gtcgtgtcga caacgatcac cgggcgcgcc gtcccctcgc
     2521 tcgaagcccg tgtcgccggg cggatcgggc tgcgcaccga cgtcaagcgc gtccctctcg
     2581 tggggctcgg ctgcgtggcc ggagccgccg ggatcgcccg cgcccacgac tacctgctgg
     2641 ggcacccggc cgaggtagcc gtcctcatgt cggtcgagct gtgcagcct
//
LOCUS       DATAIX010000610         1879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_254696, whole genome shotgun sequence.
ACCESSION   DATAIX010000610 DATAIX010000000
VERSION     DATAIX010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254696"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1879)
                     /locus_tag="VHM65_08980"
     CDS             complement(<1..>1879)
                     /locus_tag="VHM65_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX2705880.1"
                     /translation="TPAMRGLRAPWVEGIVFLSNPELDVDGLAATGRANVFGRPNHAG
                     LPDLLEHLRGGGSVDAASGRRLAQAVEQAGIRQSPRARRVGTLQMAMPALQEGPGWQD
                     FRARHERFPDDPPRRVRIYLVGSATTPDQRGKLVRAAEREYKALRGIEFPGISAPIDF
                     VEHDLGPALVFPYDEGFARLDHFLTEHGTRMSVDERLALVRSVAETIDYAHRRVLTHR
                     SLSPSCIWVRRDGEGCAVQVTDWQTASRGSDTGTGVTGTRTIADLADAGASGYCAPEW
                     AWATRNGMALDVFALGATAYFILTGRAPAASYAALTQRLHMQHALHLRQQLDGVPEAL
                     DQLIADATAADPAKRTADVSAFLATLDEIRVTLKTPVEEGLPAADPLQAEPGDEIDGF
                     TVVRVLGRGSTARAFLVHRADPADVSVLKVSLDPSKDDRLRAEAEALDVLQKEPGIVH
                     LQDGPLTVGPRLAILLSYAGERTLGQELRERGRLQPEWLHDWGRDLLDVVDALERLGV
                     AHRDIKPDNLGVHERGGKATKRRLALFDFSLAEEPLAAVEAGTPPYLDPFLGSPERRL
                     WDVAAERYASAVTLYEMATGRRPEYGGSGAHPGFTDADVTVDEELFERAYAGGLAEFF
                     RR"
BASE COUNT          239 a          670 c          707 g          263 t
ORIGIN      
        1 cggcggaaga actcggccag tcccccggcg taggcccgct cgaacagctc ttcgtccacg
       61 gtgacgtcgg cgtcggtgaa gccggggtgc gcgccggacc cgccgtactc gggtcgccgg
      121 ccggtggcca tctcatagag ggtgaccgcg ctggcgtacc gctcggccgc gacgtcccac
      181 agtcgccgct ccgggctgcc gaggaacgga tcgaggtagg gcggagtgcc cgcctcgacc
      241 gctgcgagcg gctcctccgc cagcgagaag tcgaacagcg cgagccggcg cttggtggcc
      301 ttgccgccgc gctcgtggac gccgaggttg tcgggcttga tgtcgcggtg cgcgacgcca
      361 aggcgctcca gcgcatccac cacgtcgagc agatcgcggc cccagtcgtg cagccactcc
      421 ggttgcagcc gacctcgctc ccgcagctcc tggccgagcg tgcgctcccc ggcgtacgac
      481 agcaggatgg ccagccgggg gccgacggtg agcgggccat cctggaggtg gacgatgcct
      541 ggctctttct gcaggacgtc cagggcctcg gcctccgcgc gcagccggtc gtccttgctc
      601 gggtccagcg acaccttgag cacgctgacg tcggccgggt ctgcgcggtg caccaggaac
      661 gcccgcgcgg tcgagccgcg gcccagcacg cggaccacgg tgaacccgtc gatctcgtcg
      721 ccgggctcgg cctgcagcgg gtccgcggcc ggcagcccct cctcgaccgg cgtcttcagc
      781 gtcacccgga tctcatccag cgtggccagg aacgccgaca cgtctgcggt gcgcttcgcc
      841 gggtccgcgg cggtggcatc agcgatcagc tggtcgagcg cctcgggtac gccgtcgagc
      901 tgttggcgca ggtgcagggc gtgttgcatg tgcagccgct gggtgagggc ggcgtaggag
      961 gcggccggcg cccggcccgt gaggatgaag tacgccgttg cgccgagcgc gaagacgtcg
     1021 agcgccatcc cgttgcgggt ggcccaggcc cattccggcg cgcagtagcc gctcgcaccc
     1081 gcgtccgcca ggtcggcgat cgtgcgggtg ccggtcacgc cggtgccggt gtccgatccg
     1141 cgcgacgcgg tctgccagtc ggtgacctgg acggcgcagc cctcgccgtc gcggcgtacc
     1201 cagatgcagc tcgggctgag gctgcggtgg gtgagcaccc ggcgatgcgc gtagtcgatg
     1261 gtctccgcaa ccgagcggac cagcgcgagc cgctcgtcga cggacatcct ggtgccgtgc
     1321 tcggttagga agtggtccag ccgggcgaag ccctcgtcgt acgggaagac cagggccggg
     1381 ccgaggtcgt gctcgacgaa gtcgatgggc gcggaaatgc cggggaactc gatgccgcgc
     1441 agggctttgt actcccgctc ggcggcgcgg accagcttcc cgcgctggtc gggcgtggtc
     1501 gcgctaccga ccaggtagat ccgcacccgg cgcggtggat catcggggaa ccgctcgtgc
     1561 cgggcccgga agtcttgcca gcccggccct tcctgcagcg ccggcatcgc catctgcagg
     1621 gtgccgaccc ggcgggcgcg cggggactgc cggatgccgg cctgctcgac ggcctgggca
     1681 agccggcggc cggacgcggc gtcgacggag ccgccgccgc gcaggtgctc cagcaggtcc
     1741 gggagcccgg cgtggttcgg tcggccgaag acgttcgctc gcccggtggc ggccagcccg
     1801 tccacgtcga gctcgggatt gctgaggaag acgatcccct caacccaggg cgcccgcagc
     1861 ccgcgcatcg cgggcgtcg
//
LOCUS       DATAIX010000611         2697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_226068, whole genome shotgun sequence.
ACCESSION   DATAIX010000611 DATAIX010000000
VERSION     DATAIX010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226068"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VHM65_08985"
     CDS             <1..943
                     /locus_tag="VHM65_08985"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX2705881.1"
                     /translation="GRAHLDEMMLRALTVGWAGLLYRDISFGGATLVRWDAMLGTNRW
                     LSAMAMVVVSGTGFLLYVAGAGLVASSREHTPLGPTIRDEFQVAFGVTSALSATGALI
                     ALAARPVGLLALPLYLVPLLLTQFAVRRQGTISETYRQTIRTLSHMTDVGGYTSPGHA
                     ERVADLSLSVGRAYGMRERDLRDLEFAALLHDVGQVALFEPIPGGATVLAAPADQRRI
                     AADGAQIVRETGVLKTVVEILEAQTHQYRQVREFGEVLPLESRIIKVVNAFDDLTGGS
                     RDRVAEDAAVERIYLGLGYEYDPGVVEALRQVLARRH"
     gene            complement(1619..2023)
                     /gene="sodN"
                     /locus_tag="VHM65_08990"
     CDS             complement(1619..2023)
                     /gene="sodN"
                     /locus_tag="VHM65_08990"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HEX2705882.1"
                     /translation="MFSRLFAPTIDVSAHCDLPCGVYDPAQARLEAESVKACMVKAND
                     SSDADFRARALAIKEERSDLVKHHLWVLWTDYFKPAHFEKYPALSALFNEATKLAGAG
                     GTKASWDVTVADQLLAKIDQIAVVFWETKKAV"
     gene            complement(2129..2539)
                     /locus_tag="VHM65_08995"
     CDS             complement(2129..2539)
                     /locus_tag="VHM65_08995"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEX2705883.1"
                     /translation="MAAVRRILGTVAGMAALARTRGVRVGIARVSGRSMEPTLHQGDR
                     LLVLYGVPPRAGGMAVVRLPAHDQDHPVDRPLAVKRVTGRSPHAVHHWWIDRDNPHEG
                     VDSWQVGPIPDHDVKARVLLRMPDMPMSIRRRLG"
BASE COUNT          403 a          913 c          955 g          426 t
ORIGIN      
        1 cgggcgggca cacctcgacg agatgatgct ccgggcgctg acggtcggat gggccggtct
       61 cctctaccgc gacatctcct tcgggggggc gacgctggtc cggtgggacg cgatgctcgg
      121 caccaacagg tggctgtcgg ccatggcgat ggtggtcgtc tcggggaccg ggttcctgct
      181 ctacgtagcg ggtgcgggcc tcgtcgcctc gagccgggaa cacaccccgc tcggtccgac
      241 gatccgcgac gagttccagg tggccttcgg cgtgaccagt gccctcagcg cgacgggcgc
      301 cctcatcgcc ctcgccgcgc gaccggtcgg gctgctggcc ctgccgctct acctcgtgcc
      361 tctcctgctc acccagttcg cggtccggcg gcaggggacc atcagcgaga cctaccggca
      421 gacgatccgg accctgtcgc acatgaccga tgtcggaggc tacacctcgc ccggccatgc
      481 cgagcgcgtc gccgacctct cgctctcggt cgggcgtgcc tacggcatgc gggagcgaga
      541 tctgcgggac ctggagttcg cggcgctgct gcacgacgtc ggccaggtgg cgctgttcga
      601 gccgatcccc ggcggagcca ccgtcctggc agcacccgcc gaccagcggc gtatcgccgc
      661 cgacggtgcc cagatcgtca gagagactgg cgtgctcaag acagtcgtcg agatcctcga
      721 ggcgcagacc caccagtacc gccaggtgcg cgagttcggc gaggtcctgc ccctggagag
      781 ccggatcatc aaggtggtca acgccttcga cgacctcacc gggggttcgc gcgatcgggt
      841 ggcggaggac gcggcggtcg agcgcatcta cctcggtctc ggctacgagt acgacccggg
      901 cgtcgtcgag gcgctccgcc aggtcctggc ccgacggcac tagccgtgcg aggccgcccg
      961 ccgcgcgccc tacccggggg gaggccttct tggtggcgac cgggcagggc gaggggacgg
     1021 gccaggagat ggccgggctc tacctcgcgc tggccggaga ccgcctgcgc ggcgacgagc
     1081 cgctcacggt cgaccggctg atggcccagt cctaccgcca cggcctccgg atgacagccg
     1141 agtccgcggc gagggtcctg cagtcagcgc tgcgacgacc ccgaccctgc acgcctacgc
     1201 gggcagggct atgtccatgg gatcgattct cgacagggat gcccgataga tgaacagtcc
     1261 cggactgcgt tggatgctgt cgaggttgga tgctgtcgag cgaacgtcag tggcgcatca
     1321 cgacctccac ttgcccccat gtcgacccgg cgtctggcga ggtcaagttg accatctttg
     1381 ccccactcca cgtcatcggg ccgcacagtc ccggtcggtc agaccgtcat gaacgcgctg
     1441 gcggcgtacc tcgaggcggt tgggcggcat accggctcgt tgttcgtgaa cgtgtggggc
     1501 cggccgctca cgtatcgaca gtgaagccgc tctggaaggc cgcggcagac tggcggaccg
     1561 ggtgcggact gaccgggtgg tctgaccgct ccggtgcagg tcagaggccc taagaacgtc
     1621 agacggcctt cttggtctcc cagaagacca cggcgatctg gtcgatcttg gccagcagct
     1681 ggtccgcgac tgtgacgtcc caggacgcct tggtgccccc ggcgccagcg agcttggtcg
     1741 cctcgttgaa cagggcagac agggccgggt acttctcgaa gtgggccggc ttgaagtagt
     1801 cggtccagag cacccacagg tggtgcttga ccaggtcgga gcgctcttcc ttgatggcga
     1861 gggcacgggc cctgaagtca gcatcggagc tgtcgttggc cttgaccatg caggccttga
     1921 cggactcggc ctcgagccgg gcctgggcag ggtcgtagac accacacggg aggtcgcagt
     1981 gcgcgctgac gtcgatcgtg ggggcaaaga gtcgcgagaa catggaggtc ccttccgcgg
     2041 gtggatggtc ggtcgccttc aaactacctc gcggtgcacg tggcgcaaag accggcccgg
     2101 tgggtgagcc ggcagcccat cggcccggtc agcccaggcg gcgtcggatg ctcatgggca
     2161 tatccggcat tcggagcagc acccgggcct tgacgtcgtg gtccgggatc ggtcccacct
     2221 gccacgagtc gacgccctcg tgggggttgt cccggtcgat ccaccagtgg tgcacggcat
     2281 gcggggaccg gccggtcacc cgcttgacgg cgagcgggcg gtccaccggg tggtcctggt
     2341 cgtgggctgg gagccggacg accgccatac ctccggctcg gggtgggacg ccgtagagga
     2401 cgaggagccg gtcgccttgg tgcaaggtgg gctccatcga gcggccggac acgcgggcaa
     2461 tgccgacccg gaccccccgc gtccgcgcga gcgcggccat accggcaacc gtgccgagga
     2521 tgcgacgaac ggcagccatc tgtccacctc cactgacgtg acaccgggcg cgtcccacgt
     2581 cacattcggc acgtgatgcc aaggatcgat cggcagcggt gtcatcataa cggtctgcga
     2641 gtcaccccct cgcggacgtg atgtggccac acccggcttc gcccgtgcac tcagcac
//
LOCUS       DATAIX010000612         2665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_254737, whole genome shotgun sequence.
ACCESSION   DATAIX010000612 DATAIX010000000
VERSION     DATAIX010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254737"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VHM65_09000"
     CDS             <1..386
                     /locus_tag="VHM65_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705884.1"
                     /translation="PDVWEMLAPVMDAAMVDLKCLDPQIHLQLTGQPNERVLASIRHL
                     HDLGRLYEVRLLILPGLNDGDALLERTAAWLADIDPHMRLKVIGLRRHGVRPSPVTLR
                     EPAAGERSHYRDVVAQVGDFAITVV"
     gene            complement(748..1440)
                     /locus_tag="VHM65_09005"
     CDS             complement(748..1440)
                     /locus_tag="VHM65_09005"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX2705885.1"
                     /translation="MATPWPTQQLGSTGEDVRTVQYLVAVHGHPVAVDGQFGPVTRAA
                     VKAFQASKGLGADGVVGNLTWSALIVPVAPGAGGDRLRAVQGQLASQGWRLAVDGVAG
                     PQTARSIRDFQSAHGLVVDGAVGPQTWNVMVAGFARLPSPDLAATRLYDAWGSDDRVS
                     ALRSATQAAVDLLLRGTRGTLTAAGCVPDPVLGPGHFVCAYTYEGGAASLQVRGTVLD
                     GYYVESASFVAD"
     gene            1595..2365
                     /locus_tag="VHM65_09010"
     CDS             1595..2365
                     /locus_tag="VHM65_09010"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX2705886.1"
                     /translation="MAEHDESAYAAALSAGQALYPGVDIGRPGRARTYWLSIAALRGL
                     RARWDVHVDGAGNVAPGAGILIGNHVSGLDPLVAVMSSWWRVTAFTKVEVFEGRGAVF
                     FRWMGQIPLRRGDEASTQWAMDMATRTLASGGKVGLYPEGTRSPDPAKLHRLHKRVLI
                     ALLHRNPDVPVYAITTTYRRRPWRRVHVQVRLSVRLPVDARTMTADELTSVVRDALLE
                     LGGQTYVDIYARDAKRALARGADGSRSPTQSSDQKGSA"
     gene            complement(2275..>2665)
                     /locus_tag="VHM65_09015"
     CDS             complement(2275..>2665)
                     /locus_tag="VHM65_09015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX2705887.1"
                     /translation="SWVDAPRALVEVLVLSPLSVDPGCQRRGIGSALVRHALREAEHA
                     GAVAVFLEGDPAYYSRLGFRPGGELGFTRPSVRIPEAAFQVVVLPAHQEWMTGALVYA
                     DPFWSLDCVGLRDPSAPRASARLASRA"
BASE COUNT          392 a          964 c          935 g          374 t
ORIGIN      
        1 cccccgacgt ctgggagatg ctggccccag tcatggacgc cgcgatggtg gacctgaagt
       61 gcctcgaccc gcagatccac ctgcagctga ccggtcagcc caacgaacgg gtgctggcca
      121 gcatcaggca cctgcacgat ctgggccggc tctacgaggt gcggttgctg atcctgccgg
      181 gactcaacga cggtgacgcg ctcctggaga ggacggccgc gtggctggcc gacatcgatc
      241 cccacatgag gctgaaggtc atcggcttgc gtcggcacgg ggtccgtccg tcgccggtca
      301 cgctgcgcga gccggctgct ggcgagcgtt cgcactaccg ggacgtcgtg gctcaggtcg
      361 gcgacttcgc gatcaccgtc gtctgagtca tcgcggggta gaccatcgcc tcggggttct
      421 cgggcagtgt ttccgtggcc ggtgctgccc accgcggtat ggcgtgtgcg gccgttggtc
      481 ttcttccccg cgtcgtaccc gcggacgccc aaggggcgac cgtgtcgtcg acgctcatcg
      541 cgggcgggac gtccgcggac cgctggaagc cgtgggctac agaggtgggt ctggccggcg
      601 ctgaccggcc ggatcgacct cacccacgat gtcaccatga gctactcacc acagggcatc
      661 aggcaccacg cacccgtggg caactgagct cccagacgcg ctccaggcct cggctgccct
      721 cgatgcattc ggccggcacg tgacgggtca gtcggcgacg aatgacgccg actcgacgta
      781 gtacccgtcg aggacggttc ccctcacctg caagctcgcg gcgccgccct cataggtgta
      841 ggcgcacacg aagtgaccag ggccgagcac cgggtcgggg acgcagccgg cggccgtaag
      901 cgtgcctcgg gtcccgcgga ggagaaggtc gaccgcggcc tgagtggcgc tgcgcagcgc
      961 ggacacccgg tcgtctgacc cccacgcgtc gtagaggcgg gtggcagcca ggtcgggcga
     1021 cgggagcctg gcgaaccccg ccaccatgac gttccacgtc tgagggccga ccgcgccgtc
     1081 gacgaccagg ccgtgagcgg actggaagtc ccggatcgag cgggccgtct gcggaccggc
     1141 gaccccgtcg accgcgagcc gccagccctg gctcgccagc tgcccctgga ccgcccgcag
     1201 ccggtcgccg cccgcaccgg gagccaccgg gacgatgagc gccgaccagg tgaggttgcc
     1261 gacgacgccg tcggctccga gccccttcga tgcctggaac gccttcaccg ctgccctggt
     1321 caccgggccg aactgcccgt cgacggccac tgggtggccg tggacggcca cgaggtactg
     1381 gaccgtccgg acgtcctccc ctgtgctgcc gagctgctgg gtcggccatg gtgtcgccat
     1441 gttgccctcc tggcgcgctg ggcgcgcggg tgcgcgccac gtccttggaa atgctagccc
     1501 aagccccgtg gcaccggtcc atgacaccgc ccgctcgacg cgccgtccca cgacgggcgg
     1561 cccttcagcg gtgctgatcc ccgatgatga ggtcatggcg gaacacgacg agtcggcata
     1621 cgccgcagcg ctctcggctg ggcaggcact ctatcccggt gtcgacatcg gcaggccggg
     1681 ccgcgcccgc acctactggc tctccatcgc cgcgctgaga gggctgcgcg cccgctggga
     1741 cgtccacgtc gacggggccg ggaacgtcgc ccctggtgcc ggcatcctca tcggcaacca
     1801 cgtctcaggc ctcgacccgc tcgtcgcggt gatgagctcg tggtggcggg tcacggcctt
     1861 caccaaggtc gaggtgttcg aggggcgcgg tgcggtcttc ttccggtgga tgggccagat
     1921 ccccctgcgc cgcggagacg aggcatcgac ccagtgggcc atggacatgg ccacgaggac
     1981 cctggcctcc ggcggcaagg tcgggctcta ccccgagggg acccggtcac cggacccggc
     2041 gaagctccac cggctccaca aacgggtcct catcgccctg ctccacagga accccgacgt
     2101 cccggtctac gccatcacga cgacctaccg gaggcggccg tggcgccggg tgcacgtcca
     2161 ggtccgcctc tcggtccggc tgcctgtcga tgcccgcacg atgaccgccg acgagctgac
     2221 gagcgtcgtg cgtgacgccc tgctcgagct cggcggtcag acgtacgtcg acatctacgc
     2281 ccgggacgcc aagcgcgcgc tggcccgtgg tgccgacggg tcccgcagcc cgacgcagtc
     2341 gagcgaccag aaggggtcgg cgtagacgag ggcgccggtc atccactcct ggtgtgccgg
     2401 gagcacgaca acctggaagg cggcctcagg gattcgcacg ctcggccgcg tgaacccgag
     2461 ctcgccaccg gggcgaaaac cgagccggga gtagtaggcc gggtcgcctt cgaggaagac
     2521 cgcgacggcc ccggcgtgct cggcctcgcg gagggcgtgc cgcacgagtg ccgacccgat
     2581 gccgcggcgc tggcagcccg ggtcgacact cagcgggctg agaaccagaa cctcgacgag
     2641 tgcccgcggc gcgtcgaccc acgac
//
LOCUS       DATAIX010000613         3016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_196670, whole genome shotgun sequence.
ACCESSION   DATAIX010000613 DATAIX010000000
VERSION     DATAIX010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196670"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="VHM65_09020"
     CDS             <1..1156
                     /locus_tag="VHM65_09020"
                     /inference="COORDINATES: protein motif:HMM:NF021588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta-hydrolase family protein"
                     /protein_id="HEX2705888.1"
                     /translation="LTVGVVRLGADGFIRWADESSGAFDTTTAEGITRPTTSTVSSGA
                     GSLVRWQDLGYEGRSFVAGAARPGQPDSSCGGGADTAPIRAYVGLTSADTVEQRVRLA
                     VAELRRMGAFDRRVLVVVTPTGTGWVDPDAARAIECLYGGDTAMVAVQYSFLPSWIAF
                     LLDGKSPPILGRALFDAVHDEWVAHPAGTRPRLVVFGESLGSFGAESAFSRADLAGSI
                     EAMTSQVGGALFTGPTGNNPVFEQIVSGRVDDSPPWRPTLIGEPHLRVANRAADIAPG
                     QPGWPTPRVLYVHHPSDAVGTWKVESIWRAPGWAQDPAAYDIPSAATWVPLVSFVQES
                     FDLLNGFSANPGHGHDYSTDFVAAWAAVAPPAGWTPADTARLAAHLDRRP"
     gene            complement(1202..2134)
                     /locus_tag="VHM65_09025"
     CDS             complement(1202..2134)
                     /locus_tag="VHM65_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX2705889.1"
                     /translation="MSEPDGHAGPDGHTGPDGRRAGLSRLPAPALVLGAVISVQFGGA
                     IAATLVPTIGAAGSVTLRLVFSAAVLGIVARPGLRGHSPRSWVTVVLFGAALGLMNLC
                     FYASLGRLPLGVAVTIEFIGPLVLATTLSRRASDLFGVAAAAAGVVLISRALSTPWDQ
                     LDLAGLGLALAAGAFWAAYIVLSARTGAAFPELDGLALAMVAAAVLVAPFGLASVPLW
                     SPDVLMRGVGIAILSSVLPYSLELVALRRLSTRVFGVLLSLEPAAAALAGLLVLGQRL
                     SGTQLAGMALVIGASAVVLGHGTARPDGTDAHLG"
     gene            complement(2131..2877)
                     /locus_tag="VHM65_09030"
     CDS             complement(2131..2877)
                     /locus_tag="VHM65_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX2705890.1"
                     /translation="MALLLVDVLVTTMWTSPDAPRGVDALAVAAVPDPPGWLAALDRH
                     PGDDESGDGRLGLHGRVETQLLAGEPFLVSEERGSWAYGAAPWQPTSKDPRGYPGWVP
                     RAHLRPTAAAAPDPPSTTGIAVEALLAHARRHVGLAYLWGGRSELGLDCSGLVHLSCR
                     ELGLIVPRDAHDQHAACRPVALGHVQAGDLYFFAHPGRRAHHVGIVTAPGVMLHAPET
                     GQQVIEEPLSEDRRRTLVAAGRLPLLATDR"
BASE COUNT          427 a         1081 c         1134 g          374 t
ORIGIN      
        1 gctcaccgta ggcgtcgtgc ggctgggagc ggacggcttc atccggtggg cggacgagag
       61 ctcgggtgcc ttcgacacga ccacggccga ggggatcacc cggccgacga catcgacggt
      121 gtcctcgggt gcgggctcgc tggtgcgctg gcaggacttg gggtacgagg gccgcagctt
      181 cgtggcgggg gccgcccgac ccggccagcc ggactcctcg tgtggtggcg gcgcggacac
      241 cgcaccgatc cgcgcctacg tggggctgac gtccgccgat accgtcgagc agcgagtgcg
      301 gctcgcggtg gccgagctgc gtcggatggg cgccttcgac cggcgggtcc tcgtcgtcgt
      361 gacacccacc ggcaccggct gggtcgatcc cgacgcggcc cgcgcaatcg agtgtctcta
      421 tggtggtgac acggcgatgg tcgccgtcca gtattcgttc ctgcccagct ggatcgcctt
      481 cctgctcgac ggcaagagcc cgccgatcct gggcagggcg ctcttcgacg cggtccacga
      541 cgagtgggtc gctcaccccg ccgggacacg accgaggctg gtcgtgttcg gcgagagcct
      601 cggctcgttc ggtgccgagt cggccttttc ccgggcggac ctcgccgggt cgatcgaggc
      661 gatgacctcc caggtcggcg gtgccttgtt caccgggccc acgggcaaca acccggtctt
      721 cgagcagatc gtctccggcc gtgtcgacga cagcccgccg tggagaccca ccctgatcgg
      781 cgaaccacac ctgcgcgtcg ccaaccgggc cgcggacatc gcgccgggcc aacccgggtg
      841 gccgaccccc cgggtgctct acgtgcacca cccgtcggac gccgtcggca cgtggaaggt
      901 ggagtcgatc tggcgggcgc ccgggtgggc tcaggacccg gccgcctacg acattccgtc
      961 cgcggcgacg tgggtgccgc tcgtcagctt cgtccaggag tcgttcgacc tgctcaacgg
     1021 gttctcggcg aacccgggcc atgggcacga ctacagcacc gacttcgttg ccgcctgggc
     1081 agccgtggct cccccggcgg ggtggacacc ggcggacacc gctcggctgg ccgcccacct
     1141 ggaccgccgc ccgtagtcgg gccaacgctc accccaggct caccccaggc tcaccgcagg
     1201 ctcaccccag gtgcgcgtcg gtgccgtcgg gcctggcagt gccatgaccg agaaccacgg
     1261 cgctcgcacc gatgacgagc gccatcccgg ccagctgggt gccgctgagg cgctgcccga
     1321 ggacaaggag ccccgccagc gcggcggcgg cgggctcgag gctgagcagc acgccgaaga
     1381 cccgggtgct gagccggcgg agcgcgacga gctcgaggga gtagggcagc accgaggaca
     1441 ggatcgcgat accgacgccc ctcatcagca cgtcggggct ccacaggggg acactggcca
     1501 gaccgaacgg ggcgacgagc accgcggcgg cgaccatcgc cagcgcgagg ccgtcgagct
     1561 cggggaaggc ggctccggtc cgggcgctga gcacgatgta tgccgcccag aacgccccag
     1621 cagcgagcgc cagtccgagc cccgcgaggt cgagctggtc ccagggggtg gacagcgccc
     1681 tggagatgag caccaccccc gcggccgctg cggcgacgcc gaacaggtcc gaggcccgcc
     1741 gggacaaggt cgttgccagg acgagcggcc cgatgaactc gatggtcacc gcgacaccga
     1801 ggggcagccg gccgagggac gcgtagaagc agaggttcat gagcccgagt gcggcgccga
     1861 agaggaccac cgtgacccag gaacgagggc tgtgcccgcg cagccccggc cgggcgacga
     1921 tcccgagcac cgccgcgctg aacacgagcc gcagggtgac cgaccccgcg gcgccgatcg
     1981 tcgggacgag cgtcgcggcg atggcgccgc cgaactgcac cgagatgacg gcgcccagga
     2041 ccagggccgg ggcgggcagc cggctcagcc ccgctcgccg gccgtccggt ccggtgtggc
     2101 cgtccggtcc ggcgtggccg tccggctcgc tcaccggtcg gttgccagca ggggcaggcg
     2161 gcccgcggcg accagggtcc ggcggcggtc ctcgctcagc ggctcctcga tgacctgttg
     2221 cccggtctcg ggggcgtgca gcatcacgcc cggcgcggtg acgattccga cgtgatgggc
     2281 gcggcgcccc gggtgggcga agaagtagag gtccccggcc tgcacgtgcc cgagcgccac
     2341 cggcctgcac gccgcgtgct ggtcatgggc gtctcgcggg acgatgaggc cgagctcacg
     2401 gcaggagagg tgcaccagcc ctgagcagtc gagacccagc tcgcttcgcc ctccccacag
     2461 ataggccaga ccgacgtggc gccgggcgtg ggcgagcagc gcctcgacgg cgatcccggt
     2521 ggtcgagggc ggatccggcg cggccgccgc ggtgggccga agatgggcgc gggggaccca
     2581 tcccgggtag ccgcgcgggt ccttcgaggt gggctgccag ggggcggcgc catacgccca
     2641 cgatccgcgc tcctcgctga cgaggaacgg ctcgcccgcg aggagctggg tctcgacccg
     2701 tccgtgcagt ccgaggcgtc cgtctccgga ctcgtcgtcg ccgggatgac ggtcgagtgc
     2761 ggcgagccag ccgggcgggt cgggcaccgc ggccaccgcg agggcgtcga ccccgcgcgg
     2821 agcgtcgggg gaggtccaca tcgtcgtcac gaggacgtcg accagcaaca gtgccaccgc
     2881 tgcagggtaa cccctaccag cacaggcaga acgggtggcc ggccgggtcg gcgtagacgc
     2941 ggtacccgtg ctcggcgtcg tccggaggct ggccggccac ccgttctgcc tgtgctggta
     3001 ggggttaccc tgcagc
//
LOCUS       DATAIX010000614         2134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_226370, whole genome shotgun sequence.
ACCESSION   DATAIX010000614 DATAIX010000000
VERSION     DATAIX010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226370"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..616
                     /locus_tag="VHM65_09035"
     CDS             125..616
                     /locus_tag="VHM65_09035"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX2705891.1"
                     /translation="MTPVGRAPRWAAPASTTLAVVGLLVSAYLTVEHFSQGSTLACPE
                     GEVVNCAVVTASSYAYLAGIPVALLGLLFYAVLLPLCLRRVWASPDRRLDWARLGLCA
                     VGLVFVFYLVWAELYRINAICLWCTGVHVVTFLLFCVVLFAQLLREPVVVSRGRPAAR
                     RAR"
     gene            849..1079
                     /locus_tag="VHM65_09040"
     CDS             849..1079
                     /locus_tag="VHM65_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705892.1"
                     /translation="MSTLIETDGRSRAVIPGHKNQRFLLQENEDGSLLLQPASVVTDA
                     QLEYDRDPELQALLARAAQSSTVLRSRQRRQA"
     gene            1076..1339
                     /locus_tag="VHM65_09045"
     CDS             1076..1339
                     /locus_tag="VHM65_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705893.1"
                     /translation="MNSTDAYSQVADKQLDSLEAGSDVDLYNAVLDACELIFRLPAQA
                     QARSTAITTAEGIRFRLPVAGHPPYKVFWSSDGPRIEAVFPHV"
     gene            complement(1405..>2134)
                     /locus_tag="VHM65_09050"
     CDS             complement(1405..>2134)
                     /locus_tag="VHM65_09050"
                     /inference="COORDINATES: protein motif:HMM:NF024622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HEX2705894.1"
                     /translation="ELHLSLSHGQREHDLDIARVNIAGELMDLQAGGFLEDYVDPVDV
                     ATLILDRYHSLWTELTGLERFETGDRWRVDARIRRLNEQGFDIGELAISTDVGGTQIQ
                     IQPKVVDAGHHSRRLLRLTGLDVEENQARRLLNDLDAFRAGEGRQNEDEELVAHDWLA
                     TVYEPTVRAIPRNLVGKLEPAEVFHEVLEHRWYLSERAGHDVPMARAIIDYVTSILPS
                     KPDEAAIVGVDTVEMPVVAMFDDG"
BASE COUNT          310 a          751 c          710 g          363 t
ORIGIN      
        1 tcggtcctcg gcgctgccaa cgtcatctcg gcccagctgt gcacgctgac gaagaaccag
       61 cccgccgcgg tctgcgcctc gtcgggggtc aaggccggcg cgggccagct caagtgaggc
      121 gcgggtgaca cccgtcggcc gggccccacg ctgggccgcc ccggccagca ccacgctcgc
      181 cgtggtgggg ctgctcgtct cggcctacct caccgtcgag cacttcagcc aagggtcgac
      241 cctggcctgc ccggaaggcg aggtcgtcaa ctgcgccgtg gtgacggcga gctcctacgc
      301 ctatctcgcc gggatccccg tggccctgct cggactgctc ttctatgccg tgctgctgcc
      361 gctgtgcctg cgccgggtct gggcgagccc ggaccggcgc ctcgactggg cccgtctcgg
      421 actgtgcgcg gtcggcctgg tgttcgtctt ctacctcgtc tgggccgagc tctaccgcat
      481 caacgccatc tgcctgtggt gcaccggagt gcacgtcgtg acgttcctgc tgttctgcgt
      541 cgtgctgttc gcgcagctgc tgcgcgaacc cgtcgtggtc tcccgcggca ggcccgcggc
      601 caggcgggcg aggtgaccgc agcgccatac ccgccggtgg tggttgcggg ccgccgctca
      661 gccgtcgtcc agtacggcag cacgatgagg tgaggtgggt aaataccacg gggggtcctg
      721 acacattgcc gtccccgtgc aagccggcag agtccggtct attcccactt ggcccacttg
      781 cccaaagtgg gaacgggggt accctgtgtg taggcccccc accagctaca gacatggagt
      841 gtgtcgccgt gagcaccttg atcgagaccg acggtcggag ccgggccgtg atccccggcc
      901 acaagaacca gcgcttcctg ctccaggaaa acgaggacgg cagcctcctg ctgcagcccg
      961 ccagtgttgt gacggatgcg cagctggagt acgaccgcga ccccgaactg caggcgctgc
     1021 tcgcacgggc agcgcagtct tcaacggtgc tccgcagccg ccagcgccgc caggcgtgaa
     1081 ctccaccgac gcctactcgc aggtcgccga caaacagctc gactccctgg aagcgggatc
     1141 cgacgtcgac ctctacaacg cggtcctgga cgcctgtgaa ctgatctttc gcctacccgc
     1201 tcaggcccag gcgcggtcca ccgccatcac gacggccgag ggcatccgct tccgtctgcc
     1261 tgttgccggt cacccgccgt acaaggtgtt ctggtcctcc gatgggccac gcatcgaagc
     1321 tgtgttcccc cacgtctaac ggggcgaagc cccgttagcg ataccaccgt atttacccac
     1381 cctcacctca tcttgctgcc gcagttagcc gtcgtcgaac atcgcgacga ccggcatctc
     1441 gaccgtgtcg accccgacga tcgcggcctc gtccggcttc gagggcagga tcgaggtgac
     1501 gtagtcgatg atcgcccggg ccatgggcac gtcgtgcccg gcccgctcgg agaggtacca
     1561 ccggtgctcg aggacctcat ggaagacctc ggccggctcg agcttgccga cgaggttgcg
     1621 cgggatggcc cggacggtgg gctcgtagac cgtggcgagc cagtcgtggg cgacgagctc
     1681 ctcgtcctcg ttctgccggc cctccccggc acggaaggca tcgaggtcgt tgagcaggcg
     1741 gcgggcctgg ttctcctcga catcgagccc ggtgagccgc agcaggcgcc gggagtggtg
     1801 tcccgcgtcg acgaccttgg gctggatctg gatctgggtc ccgccgacat cggtcgagat
     1861 cgcgagctcg ccgatgtcga agccctgctc gttgagacgg cggatccggg cgtcgacgcg
     1921 ccaccggtcg ccggtctcga accgttcgag gccggtcagc tcggtccaca gcgagtggta
     1981 gcggtccagg atgagcgtcg ccacgtccac cgggtcgacg tagtcctcga ggaagccgcc
     2041 agcctgcagg tccatgagct cgccggcgat gttgacccgg gcgatgtcga ggtcatgctc
     2101 tcgctgtccg tgggacaacg acaggtgcag ctcg
//
LOCUS       DATAIX010000615         1863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_226380, whole genome shotgun sequence.
ACCESSION   DATAIX010000615 DATAIX010000000
VERSION     DATAIX010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226380"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1311
                     /locus_tag="VHM65_09055"
     CDS             97..1311
                     /locus_tag="VHM65_09055"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX2705895.1"
                     /translation="MREVTQSRKLAEVCYEIRGPVLDEAKRLEEEGHRILKLNIGNPA
                     AFGFEAPDEILVDVIANLPNAQGYSDSKGILSARRAVAQYFQIKKIEGVDIEDIYLGN
                     GVSELIVMAMQALLDDGDEVLIPAPDYPLWTAATALAGGRPVHYLCDESADWWPDLAD
                     LEAKITDRTKALVVINPNNPTGALYPPEVLDALAELARKYHLVLMADEIYDKILYDGA
                     THTSIAALAPDVLTMTFNGLSKAYRVAGFRSGWVGLSGPKHRARSFIEGLNILANMRL
                     CANVPSQYAIATALGGYQSITGLILPGGRLLEQRNRAWELLNAIPGVSCTKPQGALYL
                     FPRLDPQVYAIDNDEKFVLDLLREQKILVVHGTGFSWPKPDHFRIVTLPRVEALDEAI
                     GRIGDFLATYRA"
     gene            1366..>1863
                     /locus_tag="VHM65_09060"
     CDS             1366..>1863
                     /locus_tag="VHM65_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2705896.1"
                     /translation="MTTKDELDLELDLIDGAGCIEAATRVLGPEESRLPVVVARADGV
                     WVTDVDGVRYLDCAGARGSVLFGHRHPRLVAAASRQLDKVTLAGPDVHAEVVGPFARD
                     LARLAGKDRVVLALTGAEAVAAAVRSSVRWALGARGVPAQAAGVVIVAGSAHAASAAG
                     AAGTAA"
BASE COUNT          285 a          667 c          626 g          285 t
ORIGIN      
        1 caacgagagg gccggcgatc cctggtgacg cggcggggag cgaccgaccc gcgggcgggt
       61 gcgacgtcat accggatggg gtgagagcct ggactcatgc gtgaggtgac ccagtcgcga
      121 aagctcgccg aggtctgtta cgagatccgc ggcccggtcc tcgacgaggc caagcgcctc
      181 gaggaggaag ggcaccggat cctcaagctc aatatcggca acccggccgc gttcggcttc
      241 gaggcgcccg acgagatcct cgtcgacgtc atcgccaacc tgcccaacgc ccagggctac
      301 agcgactcca agggcatcct cagcgctcgc cgcgcggtcg cccagtactt ccagatcaag
      361 aagatcgagg gtgtcgacat cgaggacatc tacctcggca acggcgtgag cgagctcatc
      421 gtcatggcga tgcaggcgct gctcgacgac ggcgacgagg tcctcatccc ggcgccggac
      481 tacccgctct ggacggccgc cacggcgctg gccggcgggc gcccggtcca ctacctgtgc
      541 gacgagtcgg ccgactggtg gccggatctc gccgacctcg aggccaagat caccgaccgt
      601 accaaggcgc tcgtcgtcat caacccgaac aaccccaccg gggcgctcta cccgcccgag
      661 gtcctcgacg cccttgccga actcgcgcgc aagtaccacc tcgttctcat ggcggacgag
      721 atctacgaca agatcctgta tgacggtgcg acgcacacct cgatcgccgc gctggctccc
      781 gacgtgctca cgatgacgtt caacgggctg tccaaggcct accgggtggc cgggttccgc
      841 agcggctggg tgggcctctc cggcccgaaa caccgggccc gcagcttcat cgagggcctc
      901 aacatcctgg ccaatatgcg cctgtgcgcc aatgtgccct cgcagtacgc catcgcgacc
      961 gcgctcggtg gctaccagag catcaccggg ctgatcctgc cgggtgggcg cctgctcgag
     1021 cagcgcaacc gcgcctggga gctgctcaac gccatacccg gtgtgtcgtg cacgaaaccg
     1081 cagggggcgc tctacctgtt cccgcggctg gacccccagg tctatgccat cgacaacgac
     1141 gagaagttcg tgctggacct gctgcgggag cagaagatcc ttgtcgtgca cggcaccggc
     1201 ttcagctggc cgaagccgga ccacttccgg atcgtcacgt tgccgcgggt cgaggcgctc
     1261 gacgaggcca tcggacggat cggcgacttc ctggcgacat accgcgcttg acgtgaccgc
     1321 gccggtggtc agtccaggtg gctcggcccc cgtatcctca tcgccatgac gaccaaggac
     1381 gagctcgacc tcgagcttga ccttatcgat ggcgccggct gcatcgaggc ggccacccgg
     1441 gtgcttggcc cggaggagtc gcgtctcccg gtcgtggtgg cccgggcgga cggtgtgtgg
     1501 gtcaccgatg tcgacggggt ccgttacctc gactgcgccg gggctcgcgg gagcgtgctc
     1561 ttcggccacc gccaccctcg gctcgttgct gccgcgtctc gccagctgga caaggtcacg
     1621 ctcgccggtc cggacgtcca cgccgaggtc gtcggcccct tcgcccgcga cctggcacgg
     1681 ctggcgggca aggaccgcgt cgtcctcgcc ctgaccggcg ccgaagccgt tgccgccgcg
     1741 gtgcgctcat cggtgcgctg ggccctcggc gcgcgcggtg tcccggcgca ggccgcgggt
     1801 gtcgtcatcg tcgccggctc ggcacacgct gccagtgccg cgggagcggc gggcacggcg
     1861 gct
//
LOCUS       DATAIX010000616         1870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_196841, whole genome shotgun sequence.
ACCESSION   DATAIX010000616 DATAIX010000000
VERSION     DATAIX010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196841"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VHM65_09065"
     CDS             complement(<1..971)
                     /locus_tag="VHM65_09065"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX2705897.1"
                     /translation="MSTVLSAVAWPYANGPRHIGHVAGFGVPSDVFSRYMRMAGHDVL
                     MVSGTDEHGTPILVQADKEGVPPRELADRFNRVIAEDLVGLGLAYDLFTRTTTPNHYA
                     VAQELFTRGHLNGYLVEQTTRGAISPSTGRTLPDRYIEGTCPICGYGEARGDQCDSCG
                     NQLDATELVNPRSKVNGETPEFVETQHFFLDLPALADAIGAWLDGREASGTWRPNVIK
                     FSRNILQDVKPRAMTRDIDWGIPVPLDGWRDNPAKRLYVWFDAVVGYLSASIEWARRS
                     GDPQAWRRWWNDPAALSYYFQGKDNITFHAQIWPAELLAYAGLGSRGG"
     gene            1036..1503
                     /locus_tag="VHM65_09070"
     CDS             1036..1503
                     /locus_tag="VHM65_09070"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2705898.1"
                     /translation="MADVRETLLIDASPLAVYALVADLPRMGEWSPECERVVWRGGAT
                     HAVKGARFFGYNRVGRVRWFTYGEVVAADPGRNLAFEITFGPMRVSRWEYYFLPHQDG
                     TCTLAEEWTDRRPTWFRVPATKALGSRTRANTRGIHRTLQAVKLVAEGSPDVG"
     gene            1567..>1870
                     /locus_tag="VHM65_09075"
     CDS             1567..>1870
                     /locus_tag="VHM65_09075"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX2705899.1"
                     /translation="MPSSSHLLAFAVAAFILIQIPGPSLLFTIGRALSVGRRDALLSV
                     LGNSAGVFVQVGAVATGVGAVVATSAQVYTVLKLAGAAYLIWLGVTAIRSRHDLTTA"
BASE COUNT          246 a          646 c          676 g          302 t
ORIGIN      
        1 ccgccccgcg agccgagccc ggcgtaggcg agcagctcgg cgggccagat ctgggcatgg
       61 aaggtgatgt tgtccttgcc ctggaagtag tacgacaggg ccgcggggtc gttccaccag
      121 cgccgccagg cctgcgggtc accgctgcgc cgggcccact cgatcgaggc cgagaggtag
      181 ccgacgaccg cgtcgaacca gacgtagagc cgtttggccg gattgtcgcg ccacccgtcg
      241 agcggcaccg ggatgcccca gtcgatgtcc cgggtcatcg cccggggctt gacgtcctgc
      301 aggatattgc ggctgaactt gatgacgttg ggccgccacg tgcctgatgc ctcgcgtccg
      361 tcgagccacg cgccgatcgc gtcggcgagc gccggcaggt cgaggaagaa gtgctgggtc
      421 tcgacgaact ccggcgtctc gccgttgacc ttgctccggg ggttgacgag ctcggtcgcg
      481 tcgagctggt tgccgcagct gtcgcactgg tcgccgcgcg cctcgccata cccgcagatc
      541 gggcaggtgc cctcgatgta gcggtcgggc agggtccggc cggtcgacgg gctgatcgcg
      601 ccccgggtcg tctgctcgac gaggtagccg ttgaggtgtc cgcgggtgaa gagctcctgg
      661 gcgacggcgt agtggttcgg cgtcgtcgtg cgggtaaaca ggtcgtaggc gagcccgagc
      721 ccgacgaggt cctcggcgat gacccggttg aaccggtcgg ccagctcgcg gggcggcacg
      781 ccctccttgt cggcctggac gaggatcggg gtgccgtgct cgtccgtgcc gctcaccatg
      841 agcacgtcgt gcccggccat gcgcatgtac cggctgaaca cgtcggaggg cacgccgaaa
      901 ccggccacgt gaccgatatg gcgcgggccg ttggcatacg gccaggcgac cgcggacagg
      961 acggtactca ttgcgccgat cctatgacct ggcgtcaccc gggtttcccg gctcgcccag
     1021 tggttggatg gggtcatggc cgacgtccgc gagaccctgc tcatcgacgc gtctccgctc
     1081 gcggtgtacg ccctcgtcgc ggacctgccc cggatgggcg agtggtcccc cgagtgcgag
     1141 cgagtcgtat ggcgcggcgg ggcgacccac gcggtcaagg gggcccggtt cttcggctac
     1201 aaccgggtcg gccgggtgcg atggttcacg tatggcgagg tcgtcgcggc cgacccgggc
     1261 cgcaacctcg ccttcgagat caccttcggg ccgatgaggg tctcccggtg ggagtactac
     1321 ttcctgcccc accaggacgg cacgtgcacg ctcgccgagg agtggaccga ccgtcggccg
     1381 acgtggttcc gggtgcccgc gaccaaggcc ctcgggagcc ggacccgggc caataccagg
     1441 gggatccacc gaaccctgca agcggtcaag ctggtcgccg agggctcccc cgacgtcggc
     1501 tgagccctgg ccggcgcccg cgggatgacg ttgcccgcgt gccggtgcgc cagccacact
     1561 ggcctcgtgc cctcgtcctc acatctgctc gcgttcgccg tcgcggcgtt catcctgatc
     1621 cagatcccgg ggcccagcct gctcttcacg atcgggcgtg ccttgtcggt gggtcgacgc
     1681 gacgcgctgc tgagcgtgct cggcaactcc gcgggcgtct tcgtccaggt cggcgcggtc
     1741 gcgaccggcg tcggggccgt cgtggccacc tcggcgcagg tctacaccgt gctcaagctc
     1801 gccggcgcgg cctacctcat ctggctgggg gttaccgcga tccggagccg gcacgacctg
     1861 accacggcac
//
LOCUS       DATAIX010000617         3747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_226461, whole genome shotgun sequence.
ACCESSION   DATAIX010000617 DATAIX010000000
VERSION     DATAIX010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226461"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..409)
                     /locus_tag="VHM65_09080"
     CDS             complement(128..409)
                     /locus_tag="VHM65_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002530052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX2705900.1"
                     /translation="MAGYTGTKDDYLKRLRRIEGQVRGLQRQVIEDTYCIDVLTQISA
                     VTKALQAVSLGLLEDHMVHCVRDAARDSDEAGAAKVAEATAAIARLVRS"
     gene            complement(608..1021)
                     /locus_tag="VHM65_09085"
     CDS             complement(608..1021)
                     /locus_tag="VHM65_09085"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX2705901.1"
                     /translation="MARTRKARPEDIHAIASALPEVELGTSWGDRPTYKVGGRGFVLY
                     RAPRHDAVDPATGHELTDLVVILTATAEDKAALVGADGPFFTIDHFRSYNAVLVSQAR
                     LGEISRDELAEVITDAWLARAPKRLAAPFLAKPAR"
     gene            complement(1054..2487)
                     /gene="purB"
                     /locus_tag="VHM65_09090"
     CDS             complement(1054..2487)
                     /gene="purB"
                     /locus_tag="VHM65_09090"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF006764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX2705902.1"
                     /translation="MLPLGDAQPPIALGALDGRYRATVADLVDHLSEAALNRERVRVE
                     VEWLIHLTSASVVPGVRALTAAEQAALRGAVDSFGPPEVAELAEIERETVHDVKAVEY
                     FLKRRLAVLAPSPGDAGLAELVHFACTSEDVNNLAYALMVRGGVREVWLPRAQALTDV
                     VAALAGELRAMPMLSHTHGQPATPTTLGKELAVLAYRLRRQLRRVEGAEYLGKLNGAT
                     GTYGAHLAAVPGADWPAVSRSFVEGLGLTWNPLTTQIESHDWQAELYADVARFNRVLH
                     NLCTDAWTYISMGYFAQVRGQGTVGSSTMPHKVNPIRFENAEANLEVSNALLDVLGST
                     LVSSRLQRDLTDSSMQRNIGSAFGHSLLAIDNVSRGLAGLDPVPVAMAADLEANWEVL
                     GEPVQSVMRALAAQGVPGMDRPYERLRELTRGRRITGADLREFIRGLGLPPDVEARLC
                     ALTPATYVGLAPQLVDYLDSDTPTRRT"
     gene            2486..3319
                     /locus_tag="VHM65_09095"
     CDS             2486..3319
                     /locus_tag="VHM65_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705903.1"
                     /translation="MALILPDATRPPPIALAATLDEDGRVLTVEARYNGPPDSGHGGI
                     TAGRLAALVPVGSATAVVVRLHAPVPLDTRLATDRQPDGSVLAADGDRLIATARELPG
                     PLPVGAFPRLAATEVARGEQAWTQARTRWDPFPDCYGCGQGRRDGIALRPGLVPGYDV
                     HATSWVPHVPDGIPGQPGGTPGQPGGIPGWLVWGVLDCATAGPVLEAADRTASVLTGE
                     LAVAQLGPVAPGHPQVLMSRATGRSGRKIRTEAVLLDPDGAVLALAQATWFIVAKDPT
                     G"
     gene            complement(3378..>3747)
                     /locus_tag="VHM65_09100"
     CDS             complement(3378..>3747)
                     /locus_tag="VHM65_09100"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2705904.1"
                     /translation="ELHTRDYDGAKAFYGAMLGHSHTEIGDGDSFVYSTIHLPGAPEA
                     GVGGVMRETSMPPDRPPYWMTYFAVEDPDATVARATELGASVMFGPEDTPYGRMAGVM
                     GPSGEMFSVMRPAPRAATVG"
BASE COUNT          526 a         1267 c         1393 g          561 t
ORIGIN      
        1 acagtgcggt gagctcctgg gtgaccgcgc tcgtgcagtg gccgcacgtc atgccggtca
       61 cggtcagggt ggtcatggtc atggtggtct ctctcgatgg tgggttcggc tgcgggtcgg
      121 cggcgggtca gctgcggacg aggcgggcaa tcgcggcggt ggcctcggcg accttggcgg
      181 caccggcctc gtcagagtcc cgggccgcgt cgcggacgca gtggaccatg tggtcctcga
      241 gcaggcccag gctgacggcc tgcagtgcct tggtgacggc gctgatctgg gtgagcacgt
      301 cgatgcagta ggtgtcctcg atgacctgac gctgcaggcc gcgcacctga ccctcgatgc
      361 ggcgcagccg cttgagatag tcgtccttgg tgccggtata gcccgccata ggtcctcctt
      421 gggtgccgga tcggctccaa ctataccccc atggggtata aagggccagt gccccaacat
      481 ccgaacaggc agccccaaca tccgaacagg caatagggcc gcgcatagtg gcatgaatgg
      541 gtgcaaaagg gactagtcgt cgaggtttgg ccagttgccc tggctcggac aggggtgaag
      601 gggacggtca gcgagccggc ttggcgagga acggcgcggc gaggcgcttc ggagcacgcg
      661 ccagccaggc gtcggtgatg acctcggcga gctcgtcgcg gctgatctca ccgaggcggg
      721 cctggctgac gagcacggcg ttatagctgc ggaagtggtc gatggtgaag aaggggccgt
      781 cggccccgac gagcgcggcc ttgtcctcgg ccgtcgcggt gaggatgacg accagatcgg
      841 tgagctcgtg tccggtggcc gggtcgaccg catcgtggcg cggcgcccga tagagcacga
      901 aaccccggcc accgaccttg tatgtcggcc ggtcacccca cgatgtaccg agctccacct
      961 cgggcagggc gctcgcgatc gcgtggatgt cctcggggcg ggccttgcgc gttctcgcca
     1021 tcccccgacg gtagaagtga tcgcggtcga ccgctaggtg cgccgagtcg gcgtgtccga
     1081 gtccaggtag tcgaccagct gtggtgcgag ccccacgtag gtcgccggcg tgagcgcgca
     1141 cagccgcgcc tcgacgtcgg gtggcagccc gaggccgcgg atgaactcgc gcaggtccgc
     1201 accggtgatc cgccgcccac gggtgagctc cctgagtcgt tcgtagggcc tgtccatgcc
     1261 cggaacccct tgtgcggcaa gcgctctcat caccgactgg accggctcgc caagcacctc
     1321 ccagttggcc tcgaggtcgg ctgccatggc cacgggaact gggtcgagcc cggccagccc
     1381 gcggctgacg ttgtcgatgg cgagcagcga gtggccgaag gcggagccga tgttgcgctg
     1441 catcgaggag tcggtgaggt cgcgctgcag tctcgagctg accaaggtcg agccgaggac
     1501 gtcgagcagg gcgttggaca cctcgaggtt ggcctcggcg ttctcgaacc ggatcgggtt
     1561 gaccttgtgg ggcatggtcg aggacccgac cgtcccctgg ccgcgcacct gcgcgaagta
     1621 gcccatcgag atgtaggtcc acgcgtcggt gcagaggttg tgcagcaccc ggttgaaccg
     1681 ggcgacatcg gcatacagct cggcctgcca gtcgtggctc tcgatctgcg tcgtgagcgg
     1741 gttccaggtc aggccgaggc cctcgacgaa cgagcggctc accgctggcc agtccgcgcc
     1801 cggcacggcg gcgagatgcg cgccatacgt gccggtggcg ccgttgagct tgccgaggta
     1861 ctcggcaccc tcgactctgc gcaactgtcg gcgcaaccgg tatgcgagga cggccagctc
     1921 cttgcccagg gtggtgggcg tggccggctg cccgtgcgtg tgcgagagca tcggcatggc
     1981 gcgcagctca ccggccagcg cggcgacgac atcggtgagc gcctgggcgc ggggcagcca
     2041 gacctcccgc acgccgcctc ggaccatgag cgcataggcc aggttgttga cgtcctcgct
     2101 cgtgcacgcg aagtggacga gctcggccag gccggcgtcg ccggggctgg gcgcgaggac
     2161 ggcgagccgg cgcttgagga agtactcgac ggccttgacg tcgtggaccg tctcgcgctc
     2221 gatctcggcc agctcggcga cctcgggggg cccgaacgag tcgacggcac cgcgcagtgc
     2281 ggcttgctcg gccgcggtca gggcgcgaac tcccggcacg acggacgcgc tggtgaggtg
     2341 gatgagccac tcgacctcga cgcggacgcg ctcgcggttg agtgccgcct cggagaggtg
     2401 gtcgacgagg tcggccacgg tggcccggta gcggccgtcg agcgcgccga gggcaatcgg
     2461 aggctgtgcg tcgccgaggg ggagcatggc gctcatcctc ccagatgcga cgaggccccc
     2521 gccgattgcc ctcgcggcca cgctggacga ggatggccgg gtgctcactg tcgaggcgag
     2581 gtacaacggg cccccggact cgggtcacgg cggcatcacc gccggtcggc tcgccgccct
     2641 cgtcccggtg gggtccgcga ctgccgtcgt cgtgcgcctg cacgccccgg tcccgctgga
     2701 cacgcggctg gccacggacc ggcaaccgga cggctcggtg ctggccgctg acggggaccg
     2761 cctcatcgcg accgcgcgcg agctgcccgg tccgttgccg gtgggcgcct tcccacgcct
     2821 ggccgccacc gaggtggccc gcggcgaaca ggcatggacc caggcgcgca cccgctggga
     2881 cccgttccca gactgctacg gctgcggcca ggggcgccgc gacgggattg ccctgcgacc
     2941 cggactcgtc cccgggtatg acgtgcatgc cacctcctgg gtcccgcacg tgccggatgg
     3001 gataccgggc cagccgggcg ggacaccggg ccagccgggc gggataccgg gctggctggt
     3061 ctggggggtt ctcgactgtg cgacagcggg tccggtcctc gaggccgcgg accggaccgc
     3121 aagcgtcctc accggggagc tggccgtggc gcagctcggg ccggtggcgc ccggtcatcc
     3181 ccaggtcctc atgagccggg cgaccggccg gtccgggcgc aagatccgca ccgaggccgt
     3241 cctgctcgac cccgacggcg cagtgctcgc cctcgcgcag gcgacctggt tcatcgtcgc
     3301 gaaggacccc accggctgac tcttcgcgca aggggccccc aggtcgcggt tccccggtgg
     3361 cggcggctag cccgcggcta gcccacggtc gccgcgcggg gagcaggccg catgaccgag
     3421 aacatctcgc cggagggccc catgactccg gccatccgcc cgtagggtgt gtcctccggt
     3481 ccgaacatga ccgacgcccc gagctcggtc gcccgagcca ccgtcgcatc ggggtcctcg
     3541 acggcgaagt aggtcatcca gtagggcggc ctgtccgggg gcatcgacgt ttcgcgcatg
     3601 acacctccca ctcccgcctc aggcgcgccg ggcaggtgga tggtcgagta gacgaaggag
     3661 tcgccgtcgc cgatctcggt gtgcgagtgc ccgagcatgg cgccgtagaa ggccttggcg
     3721 ccgtcgtagt ccctggtgtg cagctcg
//
LOCUS       DATAIX010000618         1633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_226598, whole genome shotgun sequence.
ACCESSION   DATAIX010000618 DATAIX010000000
VERSION     DATAIX010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226598"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VHM65_09105"
     CDS             <1..225
                     /locus_tag="VHM65_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705905.1"
                     /translation="ALVGSGRPARESLLAATKSVATGLVGTPYVLYGTLGQLRDQLLR
                     RRDRLGISYYAIPGHAMEAMAPLVAALAGG"
     gene            505..1134
                     /locus_tag="VHM65_09110"
     CDS             505..1134
                     /locus_tag="VHM65_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2705906.1"
                     /translation="MDDHEVVRRGLVALLERRAAFSVVAQVGTVAEALEAARTYRPDL
                     VIMDIRLPDGSGIEATREIRAENPAIRVLMLTSYPDEEAVFAAIMAGASGYLLKKIRG
                     RDLIAGLEAVGRGESLLDPAVTERVLARVRRIATGTDTDGMAQLTQQERTILLCVAEG
                     KTNKEIAAEVFLSDKTVKNYVSSILAKLNLQRRAQVAAFMVRHRYPGDE"
     gene            complement(1371..>1633)
                     /locus_tag="VHM65_09115"
     CDS             complement(1371..>1633)
                     /locus_tag="VHM65_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705907.1"
                     /translation="RQLSPRQKVAQEREARFRKLLEGLSDPAQIKAIEPEKGEKLGTL
                     RASLTKVLQGDHRGAHLAVKRGVIYVSLGPIPGARRAGGLRA"
BASE COUNT          249 a          566 c          569 g          249 t
ORIGIN      
        1 gccctcgtgg ggagcgggcg cccggcgcgc gaatcgctcc tggcggcgac caagtccgtc
       61 gcgaccgggt tggtcgggac tccctacgtc ctctacggga cgctcggtca gctgcgggac
      121 cagctgctgc gccggcggga ccgccttggg atcagctact acgcgatccc ggggcacgcc
      181 atggaggcga tggcacccct cgtcgccgca ctcgcggggg ggtagccatg accgccagct
      241 tgggggatcg ccgagccgcc tctgcgcact gcggccaggg gcaccgcaca cgacagtgcg
      301 tcgtcgcgac ccagccccgt gcggtgaggc atgccgtcgg agtcgccgcc acgctccgag
      361 ggacggtgcc cgccgaagcg gagcagcctc gcggagcgct aggatggcgc cggacctcga
      421 gcggcacgac agccggatga gccatcagga caggcgccac ccttgatcga gacgaccctc
      481 ccctcggttc tccggctcct cgtcgtggac gaccatgagg tcgttcgacg ggggctcgtc
      541 gcgctgctcg aacggcgcgc cgctttctcg gtcgtcgcac aggtcgggac cgtggcagag
      601 gcgctcgagg cggcccgtac gtatcggccc gatctcgtca tcatggatat ccgcctacct
      661 gacggatcgg gcatcgaggc gacgcgcgag atccgagccg agaatcctgc gatccgggtc
      721 ctcatgctca cgagctaccc cgacgaggaa gcggtcttcg cggcgatcat ggcgggcgcg
      781 agcggctatc tgctgaagaa gatccgcgga cgcgacctca tcgcgggcct cgaggccgtc
      841 gggcggggag agtcgctcct cgacccagcc gtgaccgaga gggtcctcgc ccgggtgcgc
      901 cggatcgcca ccgggaccga cacggacgga atggcgcagc tgacccagca ggagcggacg
      961 atcctcctgt gtgtggccga gggcaagacg aacaaggaga tcgccgctga ggtcttcctc
     1021 tcggacaaga cggtgaagaa ttacgtcagc tcgatcctcg ccaagctgaa cctccagcgt
     1081 cgcgcgcagg ttgccgcctt catggtccgc caccgttatc ccggcgacga gtaacgcgac
     1141 ggcaccagct caaacggctg cgccctcgca gatgggcggc ggccggtgcc acggatagat
     1201 aaccgtccat cacgccgagg ggcttcgtag ccctggacac gatctgctca tcgtgtgggg
     1261 ttgcgcggcc ctcctcgggg cgcccgtgat ccctccgcta tccaacttga ccgacgagac
     1321 gcggtgcgag gcggaagccg ggcccagtcg tccgcctgta gagctgtgct ctacgctcgg
     1381 agtccgccag ctctgcgcgc acccgggatg gggccgagcg agacgtagat gaccccgcgc
     1441 ttcacggcca ggtgtgcacc gcggtggtcc ccctggagaa ccttggtgag ggacgcccgc
     1501 aaggtgccca gcttctcgcc cttctcgggc tcgatcgcct tgatctgcgc ggggtcgctg
     1561 aggccctcca gcagcttgcg gaacctggcc tcgcgttcct gcgcgacctt ctggcggggc
     1621 gagagttgcc tgg
//
LOCUS       DATAIX010000619         1994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_254998, whole genome shotgun sequence.
ACCESSION   DATAIX010000619 DATAIX010000000
VERSION     DATAIX010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254998"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VHM65_09120"
     CDS             complement(<1..651)
                     /locus_tag="VHM65_09120"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX2705908.1"
                     /translation="MPHTLVAFHAHPDDEALLTSGTMACAAARGHRVVLVLATDGGLG
                     LAASAFTSDGQLAQRRRVEAVQSARALGVARVAHLGYADSGLGGSGLGGNRLGGSGLG
                     DSGLGDSGSTAQGQGAFAHADPEEAARALARILREEEAEVLLTYDANGGYGHPDHVMV
                     HHVGASAAALVGTPRVLQATVPRDTICRGVRLAGLLYRFPPDFDPTSFERAYSPSSV"
     gene            686..1867
                     /locus_tag="VHM65_09125"
     CDS             686..1867
                     /locus_tag="VHM65_09125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2705909.1"
                     /translation="MKIALLSDCYLPRLGGIEVQVHDLAERLSARGHDVEVFTATAGA
                     GCERGGTVESVDGIAVHRLAIALPWGLPVNPLAPPMLRERLRGGGFDVAHIHMGVVSP
                     FAVDCARVTSRLGLPTAMTWHCMLHWTAGVVSLLGVVRRWAEHGMAMSAVSAVAAEPL
                     RGIVGAGARVSLLPNGIDVSAWAPEPGATEPGATEPRTTEPALPVAGGGRRPVRIVTA
                     MRLARRKRPVPLLRMAQWMRALLPASVGVSLEILGDGPDRALIERHLEREAMASWVTL
                     AGRVPRADLRQRYAASDIYVSPSVLESFGIAALEARTVGLPVVARSGSGIAEFVTHGV
                     NGLLAPDDETMARQLADLVRDTERRTRIRRHNETTPPEQDWGHVVRLAEAEYARARGG
                     R"
BASE COUNT          242 a          722 c          750 g          280 t
ORIGIN      
        1 gacagagctc gggctgtagg cccgctcgaa cgaggtgggg tcgaaatccg gcgggaagcg
       61 gtagagcagg ccagccagtc ggacaccgcg acagatcgtg tcgcgcggga cggtggcctg
      121 caggacccgc ggcgtgccga ccagcgcggc cgcactggct ccgacgtggt ggaccatgac
      181 gtggtccggg tgcccgtacc cgccgttcgc gtcatacgtg agcaggacct cggcctcctc
      241 ctcacgcagg atccgtgcca gcgccctggc agcctcctcg gggtcggcgt gggcgaaggc
      301 gccttggcct tgggcagtcg atccggagtc gccgagcccg ctgtcgccga gcccgctgcc
      361 gccgagccgg ttgccgccga gcccgctgcc gccgagcccg ctgtcggcat acccgaggtg
      421 ggccacccgc gcgaccccga gtgctcgcgc gctctgcacg gcctcgaccc ggcgtcgctg
      481 ggcgagctgg ccgtcgctgg tgaacgcgga tgcggccagg ccgagcccgc cgtcggtggc
      541 gaggaccagg acgacgcggt ggccgcgcgc ggcggcgcac gccatcgtcc ccgacgtgag
      601 cagcgcttca tcgtcgggat gggcgtgaaa ggcaaccagc gtgtggggca tcggacccat
      661 cctgtctcat gtcaggctag aacccgtgaa gatcgctctg ctgtcggact gctatctgcc
      721 acggctcggg gggatcgagg tgcaggtcca cgacctcgcc gagcgcctca gtgcccgggg
      781 ccacgacgtc gaggtcttta ccgcgaccgc cggggcgggc tgcgagcggg gcggcacggt
      841 cgagtcggtc gacggcatcg cggtgcaccg gctcgcgatc gcgctgccgt gggggcttcc
      901 cgtcaacccg ctggccccgc ccatgctgcg cgagcggctg cggggcggag gcttcgacgt
      961 cgcgcacatc cacatgggtg tggtcagccc gttcgccgtc gactgcgccc gggtgaccag
     1021 tcgtctgggg ctgccgaccg cgatgacctg gcactgcatg ctccactgga ctgccggggt
     1081 cgtgtccctg ctcggcgtcg tgcggcggtg ggccgagcac ggtatggcga tgagtgcggt
     1141 ctccgccgtc gcggctgagc cgctccgtgg cattgtcggt gccggggcac gggtttcgtt
     1201 gctgcccaac gggattgacg tgtcggcctg ggcgccggag ccgggcgcca ccgagccggg
     1261 cgccaccgag ccgcgcacca ccgagcccgc cctgcccgtg gccggcggtg gacgtcggcc
     1321 tgtgcggatc gtcacggcga tgcgcctcgc ccggcgcaag cggcccgtgc cgctgctgcg
     1381 catggcccag tggatgcgcg ccctgctgcc cgcctcggtc ggggtctccc tggagatcct
     1441 cggcgacggg cccgaccgcg ccctcatcga gcggcacctc gagcgcgagg cgatggcgtc
     1501 gtgggtcacc cttgcggggc gcgtgccgcg ggccgacctg cgccagcggt atgccgcgag
     1561 cgacatctac gtgtccccgt cggtgctcga gtcgttcggc atcgcggcgc tggaggcgcg
     1621 cacggtcggg ctccccgtcg tcgcgcgcag cgggtcgggg atagccgagt tcgtcaccca
     1681 cggcgtcaac gggctgctcg ccccggacga cgagacgatg gcccgccagc tcgcggacct
     1741 cgtccgcgac accgagcggc gcacccggat ccggcgccat aacgagacga ccccacccga
     1801 gcaggactgg ggccacgtcg ttcgcctcgc cgaggccgag tacgcgcggg cccggggggg
     1861 tcggtgagcg cgacaccgag cgccacgccg ggcccgcacg gcgggaatca cgatccggta
     1921 tgtcgtcgtg cagccactcc tggggccggc gaccgagccc gtcgagcctc gtcctcgccg
     1981 agctgttggc tcac
//
LOCUS       DATAIX010000620         4336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_286895, whole genome shotgun sequence.
ACCESSION   DATAIX010000620 DATAIX010000000
VERSION     DATAIX010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286895"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VHM65_09130"
     CDS             <1..440
                     /locus_tag="VHM65_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705910.1"
                     /translation="TSDRPAVAGVITAQVSGPESSGDLAWSESAPAVTRLAGLPLSGE
                     PAGLSSGRLALSSPGVPAQAQVVTLGPSGRVVTKVVAVAADSVATVDLAGASAVWVTP
                     TAGRVYAGVLAAVKGANPPLYAVTALRDTPVETARLPVRDLTR"
     gene            complement(445..804)
                     /locus_tag="VHM65_09135"
     CDS             complement(445..804)
                     /locus_tag="VHM65_09135"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX2705911.1"
                     /translation="MRTPSERFDDLVIDAATRLERLWGKPLPDIQFAVEAVPPSDPAP
                     WEDRDLPLGRLFPARRGLASQIVLYRRPIETRAHDARDLASLVEDVVTEQVAACLGVR
                     PDELDPGYGQPGYGQPG"
     gene            767..1333
                     /locus_tag="VHM65_09140"
     CDS             767..1333
                     /locus_tag="VHM65_09140"
                     /inference="COORDINATES: protein motif:HMM:NF023430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 domain-containing protein"
                     /protein_id="HEX2705912.1"
                     /translation="MTRSSKRSDGVRIAGTGGQARGPRRPRPCLSRREAGSVTDAVTD
                     ATVRRGLPRVHPAAARRAAALTWRGYSRPVALSRQCSKTGCARLAVATLTYVYADSTA
                     VLGPLATYAEPHTYDLCALHAERLTVPLGWDVVRLPRDERPVDPHPDDLLALADAVRE
                     AGRPARPAAAAAAPPGRQVRHLRVLRDS"
     gene            1373..2842
                     /locus_tag="VHM65_09145"
     CDS             1373..2842
                     /locus_tag="VHM65_09145"
                     /inference="COORDINATES: protein motif:HMM:NF007088.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX2705913.1"
                     /translation="MPTPRLSEIIKAYDVRGLVPEQLSPTVARAIGRAFADIVARPDG
                     AGSVVLGHDMRASSPELTAAFARGVTSRGLDVTVIGLCSTDGLYYASGVLGRPGAMFT
                     ASHNPAAYNGIKLCRSGARPVGQDTGLVAIRERAQALLDGELAGEPGEEPAERTGEVR
                     EQDLLAPYATYLRSLVDLSGSRPLRVVVDAGNGMGGFTVPAVLGTAAGLDPLPVQVVP
                     LYFELDGTFPHHEANPLDPANLRDLQAAVVAHGADLGLAFDGDADRCFVVDERGEPVS
                     PSAVTALVAQREIAREVAGGTPAAEVRVVHNVISSEAVPEVVRESGAVPVRTRVGHSF
                     IKAEMARHGAVFGGEHSAHYYFRDFWFADSGMLAALHVLAALGESDGPLSALTTAYER
                     YDASGEINSTVTDSGGCTAAVRAWAVEQGCRLDDLDGLTATYPGSARRPWWRVNVRPS
                     NTEPLLRLNVEARGHATMEWAREQALGIIRSTSTEGTPR"
     gene            2839..3048
                     /locus_tag="VHM65_09150"
     CDS             2839..3048
                     /locus_tag="VHM65_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705914.1"
                     /translation="MSETSAGAPSIEPWLREILRCPACRSELADGSGPSGPELHCTGE
                     ACAKAYRIDGGVPVLLVGEARSLRD"
     gene            3064..4197
                     /locus_tag="VHM65_09155"
     CDS             3064..4197
                     /locus_tag="VHM65_09155"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX2705915.1"
                     /translation="MSPPLDEALLDDEARLGAADARGTLRALATAGAQVRTGVRCARE
                     AGIGRVAGGERPRSVLVASLAGSAVVADVLELLAGPGSPVPVWVRRNVPLPGWVGALD
                     LVIAVSESGRAAGPLALAAEAARRGAWLLTVGAPDSPLADICARAQGVHVEVARTSVS
                     ARTALWALLTPVLVASDVLGLASVDEAVLARVADRLDAVAEAARPSSEAFVNPAKSLA
                     LDLAGGVPAVLGDGPLAGVAAARTAAMLARTSRVPATWGELADGADQIVACFDGPFAG
                     RSDPFADPFLDPVTAPSLRLVLMRDQLPGTALADAVEQTARESAVAVSTLHAEPGHPV
                     ERLADLIALTDYASTYLALAQGFDPTVSAHVADLRDHTDRGLR"
BASE COUNT          505 a         1677 c         1547 g          607 t
ORIGIN      
        1 tgacctcgga ccggcccgcc gttgccgggg tcatcaccgc gcaggtgtcg ggcccggagt
       61 cctccggcga cctcgcctgg tcggagtccg ctcccgccgt gacccggctc gcgggtctcc
      121 cgctctcggg cgagccggcc gggctgagct cgggccgcct cgccctctcc tctcccggcg
      181 tgcccgcgca ggcccaggtc gtcaccctgg gaccgtccgg ccgagtcgtc accaaagtcg
      241 ttgcggttgc tgccgactcg gtagccaccg tcgacctcgc aggggccagc gccgtctggg
      301 tcacgcccac cgccggccgg gtgtatgccg gggtgctggc cgccgtcaag ggcgcgaacc
      361 cgccgctgta tgccgtcacc gccctgcgcg acaccccggt cgagaccgcg cggctaccgg
      421 tccgcgacct gacccggtga ccgctcagcc cggctgtccg tagcccggct gtccgtagcc
      481 cgggtcgagc tcgtccggac ggacccccag gcacgccgcc acctgctcgg tgacgacgtc
      541 ctcgacaagg gaggcgaggt cgcgcgcgtc gtgcgcccgg gtctcgatcg gacggcggta
      601 gagcacgatc tggctcgcca gcccccgtct cgcggggaag agccgcccca gcggcaggtc
      661 ccggtcctcc cacggcgccg ggtcgctcgg cggcaccgcc tcgaccgcga actggatgtc
      721 cggcaacggc ttgccccata gcctttccag ccgggtcgcg gcgtcgatga cgaggtcgtc
      781 gaaacgctcc gacggcgtgc gcatcgccgg caccggcggc caggcgaggg gcccgcgccg
      841 gccccgcccg tgcctgtctc ggcgagaggc tggctcggtc acggacgcgg tcacggacgc
      901 cactgtacgg cgcggtctcc ctcgggtgca cccagctgcg gcgcggcggg cggccgcgtt
      961 gacctggcgc ggctacagtc ggcccgtggc cctgtcgcgt cagtgttcca aaactgggtg
     1021 tgcccgcctc gccgtggcca ccctgacgta cgtctacgcc gactccaccg cagtcctcgg
     1081 cccgctcgcc acctatgccg agccgcacac ctacgacctc tgcgccctgc acgcggagcg
     1141 gctcaccgtc cccctgggct gggacgtcgt gcgcctgccg cgcgacgagc gtccggtgga
     1201 cccgcacccg gacgacctgc tcgccctggc cgacgccgtt cgcgaggccg gacgtccggc
     1261 acgccccgca gcggcggccg ccgccccgcc aggtcgccag gtgcggcacc tgagggtcct
     1321 gcgcgactcc tgagcgggcc ccgggcgggg gcgctcggct agggtgtggc gcgtgcccac
     1381 gcctcgtctg agcgagatca tcaaggcgta cgacgtccgc ggactggtcc ccgagcagct
     1441 gagcccgacg gtggcccggg cgatcgggcg ggcctttgcc gacatcgtcg cgaggcccga
     1501 cggggccggc tcggtcgtcc tgggccacga catgcgcgcc tcctcgcccg agctcaccgc
     1561 ggccttcgcc cgcggggtca cgagccgggg cctggacgtc accgtcatcg ggctctgctc
     1621 gaccgacggg ctctactacg ccagcggtgt cctcggccgc cctggtgcga tgttcacggc
     1681 gagccacaac ccggcggcat acaacggcat caagctgtgc cgcagcgggg cccgccccgt
     1741 cggtcaggac accggcctcg tcgcaatccg cgagcgagcc caggccctgc tcgacggaga
     1801 gctcgcgggc gagccgggcg aggagccggc cgagcgcacg ggggaggtgc gcgagcagga
     1861 cctgctcgcg ccctacgcga catacctgcg ctccctcgtc gacctgagcg ggtcgcgccc
     1921 acttcgggtc gtcgtcgacg cgggcaacgg gatgggcggc ttcacggtgc cggccgtcct
     1981 cggcacggcg gccgggctcg acccgctgcc ggtccaggtc gtgccgctct acttcgagct
     2041 cgacggtacc ttcccccacc acgaggccaa cccgctcgac ccggcgaacc tgcgtgacct
     2101 ccaggccgct gtcgtggcgc atggcgccga cctcgggctg gccttcgacg gtgacgccga
     2161 ccgctgcttc gtggtcgacg agcgcggcga gccggtcagc ccgagcgccg tcaccgcgct
     2221 cgtggcgcag cgggagatcg cccgggaggt ggccggtggc accccggccg ccgaggtgcg
     2281 ggtcgtccac aacgtcatct cctccgaggc ggtccccgaa gtggtccgcg agtcgggtgc
     2341 cgtgccggtc cgcacccggg tcgggcactc cttcatcaag gccgagatgg cccgccatgg
     2401 tgcggtcttc ggcggtgagc actcggccca ctactacttc cgcgacttct ggttcgccga
     2461 cagcgggatg ctcgcggcgc tgcacgtcct ggccgccctc ggggagtccg atggcccgct
     2521 gtcagccctc acgacggcct acgagcggta tgacgcgtcc ggcgagatca actcgacggt
     2581 gaccgacagc gggggctgca cggccgcggt gcgcgcctgg gctgtcgagc agggctgccg
     2641 cctcgatgac ctggacgggc tcaccgcgac ctacccggga tcggcccggc ggccctggtg
     2701 gcgggtcaac gttcgtccct ccaacaccga acccctcctg cggctcaacg tcgaggcccg
     2761 cgggcatgcc acgatggagt gggcccgcga gcaggccctc gggatcatca gatcgacatc
     2821 gaccgaaggg acgcccaggt gagcgagacc agcgccggag caccgtccat cgaaccgtgg
     2881 ctgcgcgaga tcctgcgctg ccccgcgtgc cgaagcgagc tcgccgacgg cagcggtccc
     2941 tcggggcccg agcttcactg caccggtgag gcgtgtgcca aggcctaccg gatcgacggc
     3001 ggggtcccgg tcctgctcgt cggcgaggcc cgctcactgc gcgactagcg cctcggtccc
     3061 cccatgagcc cgcctcttga tgaagccctc ctcgacgacg aggcccgact cggtgccgcc
     3121 gacgcgcggg ggaccttgcg ggcgctcgcc acggccgggg cccaggtccg caccggtgtc
     3181 cggtgcgccc gtgaggccgg catcggccgg gtggccgggg gagagcgccc gaggtcggtc
     3241 ctcgtcgcct cgctcgccgg gtccgcagtc gtcgcggacg tgctcgagct ccttgccgga
     3301 cccggctcgc cggtgccggt gtgggtccgg cgcaacgtgc cgctacccgg ctgggtcggt
     3361 gccctggacc tcgtcatcgc cgtgtcggag tccggccgcg cggccggacc gctcgcgctg
     3421 gccgccgaag ccgcccgccg aggggcctgg ctgctcaccg tcggagcccc ggactcgccg
     3481 ctggcagaca tctgcgcccg cgctcaaggc gtccacgtcg aggtcgcccg cacgagcgtg
     3541 tccgcgcgga cggcgttgtg ggcgctgctc acgccggtgc tcgtcgccag cgacgtcctc
     3601 ggtctcgcct cggtcgatga ggcggtgctg gcgcgagtgg ccgaccggct cgacgcggtg
     3661 gccgaggcgg cccgtccgtc gtcggaggcg ttcgtgaacc cggccaagag cctcgcgctc
     3721 gaccttgccg gcggggtccc ggccgtcctc ggtgacggcc cgcttgcggg ggtggctgcg
     3781 gcgcgcacgg ccgcgatgct ggcccgcacc tcccgggtcc cggccacctg gggtgagctg
     3841 gcggacgggg cggaccagat cgtcgcttgc ttcgacgggc cgttcgccgg gcgaagtgac
     3901 cccttcgccg acccgttcct cgacccggtg acggcgccct ccctgcgtct cgtgctcatg
     3961 cgggaccagc tgcccggcac ggcgctggca gacgcggtcg agcagacggc ccgtgagtcc
     4021 gcagttgcgg tgagcaccct gcacgccgag cccggccacc ctgtcgagcg gttggccgac
     4081 ctgatcgcgc tgaccgacta cgcaagcacc taccttgccc tcgcgcaggg tttcgacccc
     4141 accgtgtcgg cccatgtcgc ggacctgcgc gaccataccg accgcggcct gcgctagcag
     4201 ctggtcatca tcacgacgac tggctcggtg ggccggcccg tcgtcaacca gggctgcggt
     4261 gccgtcggga aaccgacatt ccgccctcgt gctgcgggcc gacctgcctt ctgcctcgac
     4321 aggccgccac gacgag
//
LOCUS       DATAIX010000621         1862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_227044, whole genome shotgun sequence.
ACCESSION   DATAIX010000621 DATAIX010000000
VERSION     DATAIX010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227044"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VHM65_09160"
     CDS             <1..260
                     /locus_tag="VHM65_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705916.1"
                     /translation="LMTFLSVLIHAAAGAAGAGLGAYALMSIAGIWKPLGTYSPAALV
                     TQPASLAAGNDVAVLWPVLTSLLLAAALVALAALMFRRTEL"
     gene            complement(374..447)
                     /locus_tag="VHM65_09165"
     tRNA            complement(374..447)
                     /locus_tag="VHM65_09165"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(411..413),aa:Asp,seq:gtc)
     gene            complement(481..553)
                     /locus_tag="VHM65_09170"
     tRNA            complement(481..553)
                     /locus_tag="VHM65_09170"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Glu,seq:ttc)
     gene            complement(840..>1862)
                     /locus_tag="VHM65_09175"
     CDS             complement(840..>1862)
                     /locus_tag="VHM65_09175"
                     /inference="COORDINATES: protein motif:HMM:NF005928.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX2705917.1"
                     /translation="VAPTAALRRIAFLLERARAGTYRVQAFRKAVDIVRALPADEVAA
                     RAAAGTLTDLAGIGEATAAVVSQAVGGELPAYLALLQEQAEPPPTDGGAELYGLLRGD
                     LHCHSDWSDGGSPIEEMALTAIELGHEWLALTDHSPRLRVANGLTTARLAQQLDVVGS
                     INTALGGNGFRLLTGIEVDILDDGTLDQTEEMLGRLDVVTASVHSKLAMAAEPMTRRM
                     VAAVRNPRVNVLGHCTGRLVEGSRGKRAQSAFDARAVFEACAGSGTAVEINSRPERCD
                     PPDELIRLAIDIGCLFAIDSDAHAPGQLDMKTYGAQRASRLGVPQERIVTTWDADRVL
                     AWASAR"
BASE COUNT          264 a          668 c          670 g          260 t
ORIGIN      
        1 ccctgatgac gttcctttcc gtgctcatcc acgccgcggc aggggctgct ggcgcaggac
       61 tcggtgcgta cgcgctgatg tcgatcgccg ggatctggaa gcccctgggc acctactcac
      121 ctgcggctct cgtcacccag cccgcatcgc tcgccgccgg gaacgacgtc gcggtgctct
      181 ggcccgtgct gacttcgctg ctgctcgcgg ccgcgctggt cgcgctcgcc gcgctcatgt
      241 tccgccgcac cgagctgtag ccgccgcgct gccctcccct ggagcgagcc ggggcgggcg
      301 atgcggaccc gcgggacgtc atacccagcg catgaggccc tgcccgaggg gacaggacct
      361 catcaccgtc gagcgacccc gaccgggctc gaaccggcga cctccgccgt gacagggcgg
      421 cgcgctaacc aactgcgcta cggggcccag atggtgctag gccggtgaat cgatagtgcg
      481 tatccccaac gggattcgaa cccgtgctgc cgccgtgaaa gggcggagtc ctaggccgct
      541 agacgatggg gacctgaggt gagtcaccgc agatcctcag ggccgccagc gagcagcgct
      601 cggatccgtc gaggactgcc aaagcatagg ggatgggtcc gaggccacca aatccggtgc
      661 gtctgccagg aggtccaagg gccgggtcaa gttactgccg ggttcaggca accatcgggg
      721 tgttgggagc gtctatatgg gtagcacgtc ccacggggca cgacgggctg gtcccgtccg
      781 tacgcagggg gcggccgggg ccggccggtc gcggccagcc cgctcagccg cccagccgcc
      841 tagcgggccg aggcccaggc caggacccgg tcggcgtccc acgtcgtcac gatccgctcc
      901 tgcggcaccc cgaggcgcga cgcgcgttgc gcgccatacg tcttcatgtc gagctggccg
      961 ggtgcatgcg cgtcggagtc gatcgcgaag aggcacccga tgtcgatcgc gagccggatc
     1021 agctcgtcgg gagggtcgca acgttccggc cgggagttga tctcgacggc ggtgcccgac
     1081 ccggcacacg cctcgaagac cgctcgggcg tcgaaggccg actgggcccg cttcccgcgc
     1141 gaaccctcga cgagccggcc ggtgcagtgc ccgaggacgt tgacccgcgg gttgcgcacg
     1201 gcggccacca tccggcgggt catcggctcg gccgccatcg cgagcttgga gtgcaccgag
     1261 gccgtcacga cgtcgagccg gcccagcatc tcctcggtct ggtcgagggt gccgtcgtcg
     1321 aggatgtcga cctctatccc ggtgagcagc cgaaaaccgt tgcctcccaa cgccgtgttg
     1381 atcgacccga cgacgtcgag ctgctgggcc agccgcgcgg tggtgaggcc gttggccacc
     1441 cgcagccgcg gcgagtggtc ggtcagcgca agccactcgt ggccgagctc gatggccgtg
     1501 agcgccatct cctcgatcgg ggagccgccg tcggaccagt ccgagtggca gtgcaggtcg
     1561 ccacgcagca gcccgtagag ctcggccccg ccgtcggtcg gcgggggctc ggcctgctcc
     1621 tggagcaagg cgaggtatgc cggcagctca ccacccaccg cctgtgacac caccgcggcg
     1681 gtggcctcgc cgatgcccgc cagatcggtg agcgtcccgg ccgcggcgcg cgcggcgacc
     1741 tcgtcggcgg gcagcgcccg gacgatgtcg accgccttgc ggaaggcctg gacccggtag
     1801 gtgcccgcgc gggcccgctc gagcaggaag gcgatccggc gcagcgcggc ggtcggcgcc
     1861 ac
//
LOCUS       DATAIX010000622         1543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_197542, whole genome shotgun sequence.
ACCESSION   DATAIX010000622 DATAIX010000000
VERSION     DATAIX010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197542"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..510
                     /locus_tag="VHM65_09180"
     CDS             82..510
                     /locus_tag="VHM65_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX2705918.1"
                     /translation="MRISDVIRSKTPGVVTVRPDDTVTRLLAVLAEHRIGAIVVSEDG
                     SDVAGIVSERDVVRRLDRDGAGILEGPGSAIMTTKVITCEPVDSVETLAGVMTDRRIR
                     HVPVLADGSLYAIVSIGDIVKHRMGALQAERDHLVGYIQQ"
     gene            600..>1543
                     /locus_tag="VHM65_09185"
     CDS             600..>1543
                     /locus_tag="VHM65_09185"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX2705919.1"
                     /translation="MTALGSPEDGTVVSARGTSRLGPYRLISPLGEGGMGLVHLALDP
                     NGRAVAIKVLRSHVAADADARLRMEREFRTLSRIRDPWVAAVIDADVFGDRPYLVTRY
                     IPGPSLDEVVRTSGRLRGPDLLRLGRGLAGALAAIHAVGVVHRDLKPANVLIHDGEPV
                     VIDFGIAHMLDDSRITMTGLVMGTPGYLSPEVVQGAAVTEATDWWGFAATLAYAASGN
                     PPFGRGPMAGVLDRVRRGEFDLSGVDPRLAPLLAAALAPDASRRPHHAEVVAALEQYA
                     HGEAVTVALGPAAVSGATGPVPSYPRTRPMPVQPTREWT"
BASE COUNT          206 a          538 c          565 g          234 t
ORIGIN      
        1 ccccccacca taggcgtgcg gggaggctga tagcggccgg gcgaggtagc ggcgcggggc
       61 tcccagggtt accttcggac tatgcggatc tcagacgtca tacgcagcaa gacccccgga
      121 gtcgtcaccg tgcgtcccga cgacactgtg acccgactgc tcgccgtgct ggcggaacat
      181 cggatcgggg ccatcgtcgt cagcgaggac ggcagcgacg tcgcggggat cgtcagcgaa
      241 cgggatgtcg tgcgaaggct cgaccgcgac ggagcgggga tcctcgaggg cccgggcagt
      301 gcgatcatga ccacgaaggt gatcacctgc gagccggtcg actcggtcga gacactcgcg
      361 ggagtcatga ccgaccgtcg catcaggcac gtcccggtcc tggccgacgg gtcgctctat
      421 gccatcgtga gcatcggtga catcgtcaag caccggatgg gcgccctgca ggccgagcgc
      481 gaccacctcg tcgggtacat ccagcagtag gccctccggc ggctgcgcac gggtgggccg
      541 gccgccatac cggctcgtcc ggacctcccg acggcgtgcg gtcgcttacc gttgtcggta
      601 tgacggcgct cggctccccg gaggacggca ccgtcgtgtc agcacgaggc acctcgcggc
      661 tcgggccgta ccgcctcatc tcgccgctcg gcgagggcgg gatggggctc gtccacctcg
      721 cactggaccc gaatggccgg gccgtcgcga tcaaggtcct gcgttcgcac gtggcggctg
      781 acgccgacgc ccggctgcgg atggagcgcg agttccgcac cctgtcgcgg atccgcgacc
      841 cttgggtggc ggcggtcatc gacgcggacg tgttcgggga ccggccatat ctcgtcaccc
      901 gttacatccc cgggccgtcg ctcgacgagg tcgtgcgcac gtcggggcgg ctgcgcggcc
      961 cggacctgct gcgcctcggt cgcggccttg ccggggcgct cgccgcgatc cacgcagtgg
     1021 gggtcgtgca tcgtgacctc aagccggcca acgtcctgat ccacgacggt gagccggtcg
     1081 tcatcgactt cggcatcgcg cacatgctcg acgactcgcg gatcacgatg accggcctcg
     1141 tcatggggac gccggggtac ctctcacccg aggtcgtcca gggtgcggcg gtcaccgagg
     1201 cgaccgactg gtgggggttc gccgcgaccc tggcgtatgc cgcgtccggg aacccgccgt
     1261 tcgggcgcgg gcccatggcg ggcgttctcg accgggtccg gcgtggggag ttcgacctgt
     1321 cgggtgtcga cccgcggctg gcgcccctgc tggcggcggc tctcgcgccg gacgcgtccc
     1381 ggcggccaca ccatgccgag gtggttgcgg cgctggagca gtacgcccat ggcgaggcgg
     1441 tcacggtggc gcttgggcct gctgcggtca gcggcgcgac cggaccggtt ccgtcatacc
     1501 cgaggactcg tccgatgccc gtccagccca cccgggagtg gac
//
LOCUS       DATAIX010000623         2492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_135872, whole genome shotgun sequence.
ACCESSION   DATAIX010000623 DATAIX010000000
VERSION     DATAIX010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135872"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /gene="trpC"
                     /locus_tag="VHM65_09190"
     CDS             <1..634
                     /gene="trpC"
                     /locus_tag="VHM65_09190"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001369.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX2705920.1"
                     /translation="VKRASPSKGVLADIGDPAALAVAYAAGGAVAVSVLTEQRRFHGS
                     LDDLDRVRAAVGIPVLRKDFTVEPYQVVEARAHGADIILLIVAALAQPALVGLHELSE
                     SLGMTALVEVHDEEETRRAVDAGARVIGVNARNLKTLEVDRSTFARLRPLVPEGVLAI
                     AESGVRDVADVAAYVAQGARGVLVGEALVTGGDPRARVAQLVSATQGARA"
     gene            631..1851
                     /gene="trpB"
                     /locus_tag="VHM65_09195"
     CDS             631..1851
                     /gene="trpB"
                     /locus_tag="VHM65_09195"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX2705921.1"
                     /translation="MTGPRMTDDSRPGRFGDFGGRYVPEALIPALEQLDEVRRKAMVD
                     PAFVGELERLHRTYTGRPSIITEVPRFAAHAGGARVILKREDLNHTGSHKINNVLGQA
                     LLTIRMGKSRIIAETGAGQHGVASATAAALLGLDCVVYMGRVDTERQALNVARMRLLG
                     AEVIPVTAGSQTLKDAVNEALRDWVTSVESTHYVLGTVTGPHPFPEMVRDFHRVIGVE
                     ARAQVLDLVGRLPDVVVACVGGGSNAIGIFHRFLDDASVRLVGCEAGGRGVHTGRHAA
                     RFAAGAPGVLHGAFTYVMQDEDGQTTPSHSVSAGLDYPSVGPEHAYLLDSGRAEYRPV
                     TDDEAMEAFALLCRTEGIIPAIESAHALAGAMAIGRDLGPDAVILVNLSGRGDKDVHT
                     AASWFGLLEQGAGS"
     gene            1848..2213
                     /locus_tag="VHM65_09200"
     CDS             1848..2213
                     /locus_tag="VHM65_09200"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX2705922.1"
                     /translation="MSAVADVLARCRAEGRAALIGYLPVGFPDVAGSIAAMTAMVESG
                     ADIIEVGIPYSDPLMDGLVIAHAAERALQGGTRVSDAFLATAAVHEAGAPALVMTYWN
                     LVLRRGVELGATRKTRSRS"
     gene            complement(2144..>2492)
                     /locus_tag="VHM65_09205"
     CDS             complement(2144..>2492)
                     /locus_tag="VHM65_09205"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX2705923.1"
                     /translation="GIPYSDPLMDGLVIAHAAERALQGGTRVSDAFLATAAVHEAGAP
                     ALVMTYWNLVLRRGVERFAADLAAGGASGLITPDLIPDEAAEWLAAADRHDLDRVFLV
                     APSSTPRRRTRFQ"
BASE COUNT          344 a          904 c          906 g          338 t
ORIGIN      
        1 ggtcaagcgg gccagcccga gcaagggagt cttggccgac atcggcgacc cggcggccct
       61 cgccgtcgcg tatgccgcgg gcggcgccgt cgcagtcagc gtcctcaccg agcagcggcg
      121 tttccacggg tccctggacg acctcgaccg cgtgcgcgcg gcggtcggca taccggtcct
      181 gcgcaaggac ttcaccgtcg agccctacca ggtcgtcgag gcccgggccc acggcgccga
      241 catcatcctg ctcatcgtcg cggccctcgc gcagccggcc ctcgtcgggc tgcacgagct
      301 gagcgagtcg ctcgggatga ccgccctcgt cgaggttcac gacgaggagg agacccgccg
      361 cgcggtcgac gccggagcgc gggtcatcgg ggtcaacgcg cgcaacctca agaccctcga
      421 ggtcgaccgc tcgacgttcg cgcggctgcg ccccctcgtc cccgaagggg tcctcgcaat
      481 cgccgagtcc ggggtccgcg atgtcgccga cgtcgcggca tacgtcgcgc agggagcccg
      541 cggggtgctc gtgggtgagg ccctggtcac cggtggggac ccgcgggcgc gggtcgccca
      601 gctcgtctca gcgacgcagg gagctcgggc atgacaggcc cacgcatgac ggacgacagc
      661 aggccgggcc ggttcggtga cttcgggggc cgctacgtgc cggaggcgct gatcccggct
      721 ctcgaacagc tcgacgaggt ccgccgcaag gcgatggtcg acccggcgtt cgtcggagag
      781 ctcgagcggc ttcaccggac ctatacgggc aggccgagca tcatcaccga ggtgccccgc
      841 ttcgcggccc acgccggtgg cgcccgggtc atcctcaaac gtgaggacct caaccacacg
      901 gggtcgcaca agatcaacaa cgtgctgggc caggctctgc tcaccattcg gatgggcaag
      961 tcccggatca tcgccgagac cggggccggc cagcacggtg tcgcgagcgc gaccgccgca
     1021 gcgctgctcg ggctcgactg tgtcgtctac atgggccggg tcgacaccga gcgccaggcg
     1081 ctcaacgtcg cccggatgcg cctgctcggc gccgaggtca tcccggtgac tgccgggagc
     1141 cagacgctca aggacgcggt gaacgaggca ttgcgtgact gggtgaccag cgtcgagtcg
     1201 acccactacg tcctcggcac ggtcaccgga ccgcacccgt tccccgagat ggtccgcgac
     1261 ttccaccggg tcatcggcgt cgaggcccgc gcgcaggtgc tcgacctcgt cgggcgcctg
     1321 cccgatgtcg tcgtggcctg tgtggggggc gggtccaacg cgatcgggat cttccaccgc
     1381 ttcctcgacg acgcgtcggt ccggctcgtc ggctgcgagg ccggcggccg gggcgtccat
     1441 accggcaggc acgcggcgcg gttcgccgct ggggcgccgg gtgtcctgca cggggccttc
     1501 acctacgtca tgcaggacga ggacggccag acgaccccgt cgcactcggt ctcggccggg
     1561 ctcgactacc ccagcgtcgg gcccgagcac gcctacctgc tcgactccgg acgggccgag
     1621 taccgcccgg tcaccgacga cgaggcgatg gaggcgttcg cgctgctctg ccgcaccgag
     1681 gggatcatcc cggccatcga gagcgcgcac gccctcgcgg gcgccatggc gatcggtcgt
     1741 gacctcgggc cggacgccgt gatcctggtc aacctgtccg ggcgcggcga caaggacgtc
     1801 cacaccgccg cgagctggtt cggcctgctc gagcaggggg cgggctcgtg agcgcggttg
     1861 ccgacgtgct cgcccgctgc cgggccgagg ggcgagccgc cctgatcggc tacctcccgg
     1921 tcggcttccc ggacgtcgcc ggctcgatcg cggccatgac cgccatggtc gagtccggcg
     1981 ccgacatcat cgaggtcggc atcccctaca gcgacccgct catggacggg ctcgtcatcg
     2041 cgcacgcggc cgagcgcgcc cttcagggcg gcacgcgggt gtcagatgcc ttcctcgcca
     2101 cggcggccgt gcacgaggcg ggagcccccg cgctcgtcat gacctactgg aacctcgtcc
     2161 tgcgccgggg ggtcgagctc ggagcgacga ggaagacccg gtcgaggtca tgacggtcgg
     2221 ccgcggccag ccactccgct gcctcgtcgg ggatgaggtc gggggtgatg agcccggagg
     2281 caccaccggc cgcgaggtcc gcggcgaagc gctcgacccc ccggcgcagg acgaggttcc
     2341 agtaggtcat gacgagcgcg ggggctcccg cctcgtgcac ggcggccgtg gcgagaaagg
     2401 catccgacac ccgcgtgccg ccctgaaggg cgcgctcggc cgcgtgcgcg atgacgagcc
     2461 cgtccatgag cgggtcgctg taggggatgc cg
//
LOCUS       DATAIX010000624         5223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_255191, whole genome shotgun sequence.
ACCESSION   DATAIX010000624 DATAIX010000000
VERSION     DATAIX010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255191"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /gene="whiA"
                     /locus_tag="VHM65_09210"
     CDS             <1..369
                     /gene="whiA"
                     /locus_tag="VHM65_09210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEX2705924.1"
                     /translation="VLAWEERRMRREVRATANRLANFDDANLRRSARAAVAAGARVER
                     AVEILGEEIPDHLREAGVLRLEHKEASLEELGQLAQPPMTKDAVAGRIRRLLAMADKR
                     ASDLGIAGTDANLTQDMLDA"
     gene            578..1585
                     /gene="gap"
                     /locus_tag="VHM65_09215"
     CDS             578..1585
                     /gene="gap"
                     /locus_tag="VHM65_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX2705925.1"
                     /translation="MTVRVGINGFGRIGRNFYRAVQASGADIEIVAVNDLADNKTLAH
                     LLKYDSVLGVLDGDVSATEDSITVGDTTIKAFAERDPGALKWGDLGVDVVIESTGLFT
                     DATKAVVHKNNGAKKVIISAPAKNEDATFVMGVNHQSYDPATHHVISNASCTTNCLAP
                     MAKALYDGLGIVKGLMTTIHAYTQDQNLLDAPHSDLRRARAAALSIIPTSTGAAKAIG
                     LVMPELKGKLDGYALRVPVPTGSATDLTFEAGRETTVEEVNALVKAAAEAGPLKGYLQ
                     YTEDPIVSADIVTNPHSCIFDSGLTKVIGNQVKVVGWYDNEWGYSNRLIDLVVLVGSA
                     L"
     gene            1617..2837
                     /locus_tag="VHM65_09220"
     CDS             1617..2837
                     /locus_tag="VHM65_09220"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX2705926.1"
                     /translation="MGDLLSGGGLQGRRVLVRSDLNVPLDGETITDDGRIRASIPTIS
                     ALSEAGARVVVCAHLGRPKGAPEARYSLAPVARRLGELLGRPVAFATDTVGESARAAV
                     AGAGDGDVVLLENLRFNAGETAKTPQGRAEFAAALAGLADAFVSDGFGVVHRAQASVY
                     DVAQILPAYVGGLVEKEVGVLRRLTVDPARPYAVVLGGAKVSDKLGVIGNLLGTADRL
                     LIGGGMVFTFLAAQGHEVGTSLLEADQIETVKGYLEDAAGRGVQIILPVDIVAASAFS
                     ADAEHDVVAADAIPADRMGLDIGPASASLFARALADCRTIFWNGPMGAFEMAPYAAGT
                     LALAQALADATAAGALTVVGGGDSAAAVRQLGFADDQFGHISTGGGASLEYLEGKELP
                     GITVLESAQSDPKE"
     gene            2843..3655
                     /gene="tpiA"
                     /locus_tag="VHM65_09225"
     CDS             2843..3655
                     /gene="tpiA"
                     /locus_tag="VHM65_09225"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX2705927.1"
                     /translation="MAQEALVSAGRTPLMAGNWKMNLDHVQATHLVQKLDWTLKDAKH
                     DFGAVEVVVLPPFTDLRSVQTLVDGDKLELRYGAQDVSRHTAGAFTGEISGAFLAKLG
                     CTYAVIGHSERREYHAESDELLNAKVKAAFEHGLVPILCCGEGLDVRKQGRQVEHVLA
                     QLEVDFSDVPQEQATRIVVAYEPIWAIGTGEVATPADAQEVCGAIRTWLGSRYSADTA
                     AAIRILYGGSVKSGNVAAIMAEEDVDGALVGGASLDAVEFAGICRFRQHVTG"
     gene            3715..3960
                     /gene="secG"
                     /locus_tag="VHM65_09230"
     CDS             3715..3960
                     /gene="secG"
                     /locus_tag="VHM65_09230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX2705928.1"
                     /translation="MNTVRIGLQGLLVISGLFLTLLILLHKGKGGGLSDMFGGGVSTS
                     LGASTVAERNLDRFTVAVAIIWFAAVVGLGLIERFVA"
     gene            4020..4370
                     /locus_tag="VHM65_09235"
     CDS             4020..4370
                     /locus_tag="VHM65_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HEX2705929.1"
                     /translation="MASGNAIRGSRVGAGPMGEAERGETAPRVFVSYWCSNGHETRPS
                     FAEEAGLVIPEHWDCPRCGFPAGQSQENPPAPPKVEPYKTHLAYVKERRSDADGEAIL
                     DEALTSLRERGLIQ"
     gene            4417..>5223
                     /locus_tag="VHM65_09240"
     CDS             4417..>5223
                     /locus_tag="VHM65_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705930.1"
                     /translation="MTAPLDPTTTSAWARLAELRDALEPDLRGWFAQDPGRAQRLTLT
                     AGDLYVDLSKNLVTDDIVAALVALGTEVGLPGRRDAMFAGERINVTEDRAVLHTALRA
                     PRGRALEVDGQDVDGDVHAVLDQVYAFAERVRSGSWTGVTGKRIETVLNIGIGGSDLG
                     PAMVYEALRSCVQPGLSCRFVSNIDPADVAGKTAGLDPETTLVIVASKTFTTLETLTN
                     ARLARSWLLGALESAGAIDGSESARDAAVAQHFVAVSTALDKVAAFGIDPQ"
BASE COUNT          786 a         1894 c         1779 g          764 t
ORIGIN      
        1 gtgctcgcct gggaggagcg ccggatgcgc cgtgaggttc gcgcgacggc caaccggctg
       61 gccaacttcg acgatgcgaa cctgcgccgg tcggcacgcg ccgcggtggc ggccggggcc
      121 cgggtcgagc gggccgtgga gatcctcggt gaggagatcc ccgaccacct gcgtgaggcc
      181 ggggtcctgc gcctcgagca caaggaggcg agcctcgaag agctcggtca gctcgcccag
      241 cccccgatga ccaaggacgc cgtcgccgga cggatccgcc gcctgctggc gatggccgac
      301 aagcgtgcct cggacctcgg gatcgccggg accgacgcta acctgaccca ggacatgctc
      361 gacgcgtgac cgggtggtgc ggcgccatcc ggcaggtggc tcccggccgg tcccggccct
      421 ggccagatgt tcatctcacc cgttggcgtg actcatccgt acctcggacg cagatgcccg
      481 gtgcggtgcc ggggactagg gttgagcctg gcaccgcagc caggaccgtg ctccgcgtcg
      541 gccctggcca cccaccatct ggaaggttgg aagaacagtg actgttcgtg ttggcatcaa
      601 cggcttcggc cggattggcc gcaacttcta ccgcgccgtg caggcctctg gcgccgacat
      661 cgagatcgtt gccgtcaacg acctcgccga caacaagacc ctggcccacc tgctcaagta
      721 cgactcggtc ctcggcgtcc tcgacgggga cgtctcggcg accgaggact cgatcaccgt
      781 gggcgacacg acgatcaagg cgttcgccga gcgcgacccg ggtgccctca agtgggggga
      841 cctcggcgtc gacgtcgtca tcgagtcgac cggtctgttc accgacgcca ccaaggcggt
      901 ggtgcacaag aacaacggcg ccaagaaggt catcatctcg gccccggcca agaacgagga
      961 cgcgaccttc gtcatgggcg tcaaccacca gagctacgac ccggcgaccc accacgtcat
     1021 ctccaacgcc tcgtgcacga cgaactgcct ggcccccatg gccaaggcgc tctatgacgg
     1081 cctcggcatc gtcaaggggc tcatgacgac gatccatgcc tacacgcagg accagaacct
     1141 gctcgacgcc ccccacagcg acctgcgccg cgcccgcgcc gccgcgctgt cgatcatccc
     1201 gacctcgacc ggtgcggcca aggccatcgg cctggtcatg cccgagctca agggcaagct
     1261 cgacggctac gccctgcggg tcccggtgcc gaccggctcg gccaccgacc tcaccttcga
     1321 ggcgggccgc gagacgacgg tcgaggaggt caacgccctg gtcaaggccg cggcggaggc
     1381 cggcccgctc aagggctacc tgcagtacac cgaggacccg atcgtgtccg ccgacatcgt
     1441 gaccaacccg cactcgtgca tcttcgactc gggtctgacc aaggtcatcg gcaaccaggt
     1501 caaggtcgtc ggctggtacg acaacgagtg gggctactcc aaccgcctca tcgacctcgt
     1561 cgtcctcgtc ggctccgcgc tctgaccggg tcgaagggga tcctgacgtg ctgacgatgg
     1621 gcgacctgct ctccggtggc ggcctgcagg ggcgccgtgt gctcgtgcgg tccgacctca
     1681 acgtcccgct ggacggcgag acgatcaccg acgacggccg gatccgtgcg tcgatcccga
     1741 cgatctcggc gctgtccgag gcgggcgccc gcgtcgtcgt gtgcgcccac ctcggccggc
     1801 ccaagggtgc accggaggcg aggtactccc tcgccccggt cgcccgccgc ctcggcgagc
     1861 tgctcggccg cccggtcgcg ttcgcgaccg acacggtcgg cgagtcggcc cgggctgccg
     1921 tggccggcgc cggagacggc gatgtcgtgc tgctcgagaa cctgcgcttc aacgcggggg
     1981 agacggccaa gaccccgcag ggccgggccg agttcgcggc tgcgctcgcg ggcctcgcgg
     2041 acgccttcgt ctccgacggc ttcggcgtcg tccaccgggc ccaggcgtcg gtgtatgacg
     2101 tcgcccagat cctcccggcg tacgtcggtg ggctcgtcga gaaggaggtc ggcgtgctgc
     2161 gccggctcac cgtcgacccc gcccgcccgt atgccgtcgt cctcggcggg gcgaaggtgt
     2221 ccgacaagct cggcgtcatc ggcaacctcc tgggcacggc cgaccggctg ctcatcggcg
     2281 gcgggatggt cttcaccttc ctcgccgccc agggccacga ggtcggcacg agcctgctcg
     2341 aggccgacca gatcgagacc gtgaagggct acctcgagga cgccgcgggc cgcggggtcc
     2401 agatcatcct gcccgtcgat atcgtcgcgg cgagcgcctt ctcggccgac gccgagcacg
     2461 acgtcgtcgc cgccgacgcc atcccggccg accgcatggg tctggatatc ggtcccgcct
     2521 cggcgtcgct gttcgcccgc gcgctcgcgg actgcaggac gatcttctgg aacggtccga
     2581 tgggggcctt cgagatggcc ccgtatgccg ccggaaccct ggccctggcc caggcgctcg
     2641 ccgacgcgac ggccgctggt gcgctgaccg tcgtcggtgg gggcgactcg gccgccgccg
     2701 tgcgccagct cggcttcgcc gacgaccagt tcggtcacat ctcgaccggc ggcggagcca
     2761 gcctggagta cctcgagggc aaggagctcc cggggatcac cgttcttgag tcggcccagt
     2821 ccgacccgaa ggagtaaccg acatggcaca ggaggcactc gtgagcgctg ggcggacccc
     2881 gctcatggcc ggcaactgga agatgaacct cgaccacgtg caggccactc acctggtcca
     2941 gaagctcgac tggaccctca aggacgccaa gcacgacttc ggtgccgtcg aggtggtcgt
     3001 gctaccgccg ttcaccgacc tgcgctcggt ccagaccctc gtcgacgggg acaagctcga
     3061 gctgcgctac ggcgcgcaag acgtgtcgcg tcataccgcc ggggcgttca ccggcgagat
     3121 ctccggcgcc ttcctcgcca agctcggctg cacgtatgcc gtcatcgggc acagcgagcg
     3181 ccgcgagtac cacgcggaga gcgacgagct gctcaacgcc aaggtcaagg ccgcgttcga
     3241 gcacggcctg gtcccgatcc tgtgctgcgg cgagggcctc gacgtgcgca agcagggccg
     3301 tcaggtcgag cacgtcctgg cccagctcga ggtcgacttc tccgacgtcc cgcaggagca
     3361 ggctacccgg atcgtcgtcg cctacgagcc catctgggcg atcggcaccg gcgaggtcgc
     3421 gacccccgcg gatgcccagg aggtgtgcgg cgcgatccgc acgtggctcg ggtcgcgcta
     3481 ctccgcagac acggccgccg cgatccggat cctgtatggc gggtcggtca agtcgggcaa
     3541 tgtggcggcc atcatggccg aggaggacgt cgacggcgct ctcgtcggtg gcgcctcgct
     3601 cgacgcggtc gagttcgccg ggatctgccg gttccgccag cacgtcaccg gctgagtgcc
     3661 gcagcaggta tccttcaacc gaacaacgga cggcgaacta gtgagggcac tcgggtgaac
     3721 acggtacgca tcgggctgca ggggctgctc gtgatatccg ggctcttcct cacgctgctg
     3781 atcctgctcc acaagggcaa gggcggcggc ttgtcggaca tgttcggtgg aggtgtgtcg
     3841 acctcgctcg gcgcctcgac cgtggccgag cgcaacctcg accgtttcac ggtcgcggtg
     3901 gccattatct ggttcgccgc ggtcgtcggc ctgggcctga tcgagcgctt tgtcgcctga
     3961 gcgggccgac gtctgcgccc accagctcag tcctgcctca ccgacgaagg agttccaccg
     4021 tggccagcgg taatgcgatc cgcgggagtc gagtcggggc tgggcccatg ggcgaggccg
     4081 agcggggcga gactgccccc cgggtcttcg tgtcctactg gtgctcgaac ggccacgaga
     4141 cccgccccag cttcgccgag gaggccgggc tcgtgatccc cgagcactgg gactgccccc
     4201 gctgcggctt cccggccggg cagagccagg agaaccctcc cgcgccgccc aaggtcgagc
     4261 cgtacaagac ccacctggcc tatgtcaagg aacggcgcag cgacgccgat ggcgaggcca
     4321 tcctcgacga ggctctgacg agcctgcgcg agcgcggcct gatccagtag ccgtcccccc
     4381 caccggtcca gcacccgatc gaggagccac cccgagatga ccgcgccctt ggacccgacc
     4441 actacgagcg cctgggcccg gcttgccgag ctgcgcgacg cgctggagcc ggacctgcgt
     4501 ggctggttcg cgcaggaccc tggccgagcc cagcggctga ccctgacggc gggcgacctg
     4561 tatgtcgacc tgtccaagaa cctcgtcacc gacgacatcg tcgcggccct cgtcgcactg
     4621 ggcaccgagg tcggcctacc cgggcgccgg gacgcgatgt tcgccggcga gcggatcaac
     4681 gtcaccgagg accgtgcggt cctgcacacg gcactgcgtg cgccccgggg gcgagccctc
     4741 gaggtcgacg ggcaggacgt cgacggcgat gtccacgcgg tgctcgacca ggtctatgcc
     4801 tttgccgagc gggtccgctc cgggtcatgg accggcgtca ccggcaagcg catcgagacg
     4861 gtcctcaaca tcggcatcgg cgggtccgac ctcgggccgg ccatggtcta cgaggcactg
     4921 aggtcctgcg tgcagcccgg tctgtcctgc cggttcgtct ccaacatcga ccctgccgac
     4981 gtggccggga agacggccgg cctcgacccg gagaccaccc tcgtcatcgt cgcgagcaag
     5041 accttcacga cgctggagac cctgaccaac gcccgcctcg cccggtcctg gctcctcggg
     5101 gcgctggagt cggccggggc gatcgacggc tcagagtcag cccgcgacgc cgccgtggcc
     5161 cagcacttcg tcgcggtgtc gacggccctg gacaaggtcg ccgccttcgg catcgacccg
     5221 cag
//
LOCUS       DATAIX010000625         4281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_287349, whole genome shotgun sequence.
ACCESSION   DATAIX010000625 DATAIX010000000
VERSION     DATAIX010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287349"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..529)
                     /locus_tag="VHM65_09245"
     CDS             complement(137..529)
                     /locus_tag="VHM65_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705931.1"
                     /translation="MSRVENAVARPVRGDVAAWVAGVAGPIGELADDFGEHIEVTEGP
                     AGLYQDLMTVAPRLSGTINRLIEEHQTFHGLFATIASLEASAAKDPEHVDELRETVIR
                     LLALLTRHRARGSELVWEAYTFDVGGEM"
     gene            complement(734..2053)
                     /locus_tag="VHM65_09250"
     CDS             complement(734..2053)
                     /locus_tag="VHM65_09250"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:NF004624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEX2705932.1"
                     /translation="MTEGSAPDELLRFDREHVWHPYSSALTPGTPALVESASGVRLRL
                     RLPSGERREVIDAMSSWWCAIHGYAVPDLDAAARDQLDRMSHVMFGGLTHEPAIALAR
                     ELLDLAPRHTGSAPLEHVFFADSGSVSVEVAMKMALQCQLGRGRRRTRFFTIRGGYHG
                     DTFSPMSVTDPVGGMHSLFTGILPEHIFAPQPPAGLDSDLGDWEVRTRSLYAEHADEV
                     AAVILEPVLQGAGGMHVYNPAAVRVLCELARQHGALVIFDEIATGFGRTGTMWGADRV
                     GVTPDILCVGKALTGGYLTLAAVLCTGEVARGVSSAAAGVLMHGPTFMANPLACSVAR
                     ASLRLLGERDALAQVARLERGLAAGLEPARTLASVHDVRVLGGVGVIALREPVRGAEV
                     TAAAIELGVWVRPFRDLVYTMPPYVTSPDDLATITSTMVEAVRAIHG"
     gene            complement(2298..3371)
                     /gene="bioB"
                     /locus_tag="VHM65_09255"
     CDS             complement(2298..3371)
                     /gene="bioB"
                     /locus_tag="VHM65_09255"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX2705933.1"
                     /translation="MTAQAVHNAVPNINGPESSAGASTPGSDGILGVARGQVLERGER
                     LQYDQILEVLRTGDDQLEELLALAHAVRMAYNGPDVEVEGIVSLKTGGCPEDCHFCSQ
                     SGQFTSPVRSVWLNIPELVRAARSTRETGASEFCIVAAVRGPDARLMAQMREGVAAIK
                     AEVDIHIAASIGMLTQDQVGELVEMGIHRYNHNLEAAASYFPHVVTTHSWQERWDTCQ
                     MVKDSGMELCCGGLVGMGETLEQRAELADQLGELEPHEVPLNFLNPRPGTPFADLPIM
                     ESNDALRCIAAFRLALPRTILRYAGGRELTLGDLGTRDGLLGGINAVIVGNYLTTLGR
                     DPKEDLSLLADLQMPIKALNATL"
     gene            complement(3447..3881)
                     /locus_tag="VHM65_09260"
     CDS             complement(3447..3881)
                     /locus_tag="VHM65_09260"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2705934.1"
                     /translation="MRSHRAGAAKTAREILREARFREAVSQGELAARAGVPQSVVAAY
                     ECGAKVPTIGALQRLVGALGFELSCQLRRTTHGSSTLTGPIGQHLLHARDDVLAVLAE
                     LRLTNPRVVGDVARGEEGRLSALMFVVDDPGAPRTLRRLLAG"
BASE COUNT          603 a         1511 c         1542 g          625 t
ORIGIN      
        1 caaggcgagc agcgtctgga acgccgcagg ctcggcccgc gtcgccaagg cgcgcacgga
       61 ctccgcgtag cgtcactgaa accttgacgc tatggacggc gtcaagcctg ccttgagtgc
      121 tgcgacgggc gggtcgctac atctccccgc ccacgtcgaa cgtgtaggcc tcccagacga
      181 gctcggatcc acgggcgcgg tgccgggtga gcagggcgag gagccggatg acggtctcgc
      241 gcagctcgtc gacgtgctcc gggtccttcg cggcgctcgc ctccagggac gcgatggtgg
      301 cgaagagacc gtggaaggtc tggtgctcct cgatgagccg gttgatcgtc ccggacagtc
      361 gtggggccac cgtcatgagg tcctggtaga ggcccgccgg accctcggtc acctcgatgt
      421 gctccccgaa gtcgtcggcc agctcaccga tcgggccggc gaccccggcg acccaggcgg
      481 caacgtcgcc ccgcactggc cgcgcgacgg cgttctccac ccggctcatg gagtctcgta
      541 gctcggccct gcgacggcgc acctcgtcca gcggggcccc agacccgttc ggcgtcacgc
      601 tcacagcgca cctccagctc atcgctcacc gatatcgtct cgcgccggct tgtccagcgg
      661 tagtgccagc gcgctccctg gacaggctac tgccagcgga gcctcactcc cgcgctcaac
      721 cgtgtggggg ttctcaaccg tggatggccc ggaccgcctc gaccatcgtg ctcgtgatcg
      781 tggccaggtc atcggggctc gtgacgtacg gcggcatcgt gtagacgagg tcgcggaacg
      841 ggcgcaccca caccccgagc tcgatcgcgg ccgcagtcac ctcggccccg cggaccggct
      901 cgcggagcgc gatgacgccg acacctccca ggacgcggac gtcgtggacc gaggccagcg
      961 tgcgggcggg ctccagccct gcggcaagcc cccgctcgag cctggccacc tgggcgaggg
     1021 cgtcgcgttc gcccagcaga cgcaggctcg cccgggcgac cgagcacgcg agcgggttcg
     1081 ccatgaaggt cggtccgtgc atgaggaccc cggccgctgc cgagctcacg ccacgtgcga
     1141 cctcgccggt acacaggacg gcggccagcg tgaggtagcc cccggtcagg gccttgccga
     1201 cgcacaggat gtcgggggtc acacccaccc ggtcggcacc ccacatcgtc ccggtacggc
     1261 cgaagccggt ggcgatctcg tcgaagatca ccagcgcccc gtgctggcgg gccagctcgc
     1321 acagcacgcg cacggcggcc gggttgtaga cgtgcatgcc ccctgccccc tgcagcaccg
     1381 gctcgaggat cactgccgcg acctcgtccg cgtgctcggc atacaaggac cgcgtccgca
     1441 cctcccagtc gccgaggtcg ctgtcgagcc cggccggtgg ctggggggcg aagatgtgct
     1501 cgggaaggat ccccgtgaac agcgagtgca tgccgccgac cgggtcggtc acactcatcg
     1561 gcgagaacgt gtcgccgtgg tagccgccgc ggatcgtgaa gaaccgggtc cggcgccgcc
     1621 ccctgccgag ctggcactgc agggccatct tcatggcgac ctcgacgctg accgaccccg
     1681 agtcggcgaa gaagacgtgc tccaacggcg cagatccggt atggcgcggt gccaggtcga
     1741 ggagctcgcg ggccagggcg atggccggct cgtgggtcag gccgccgaac atgacgtggc
     1801 tcatccggtc gagctggtcg cgggcggctg catcgaggtc ggggacggca tacccgtgga
     1861 tcgcgcacca ccacgacgac atcgcgtcga tgacctcgcg acgttcgccg ctgggcagcc
     1921 gcagccgcag ccggaccccg gacgcggact cgaccagggc gggcgtcccc ggcgtcagcg
     1981 ccgaggagta gggatgccag acgtgctctc ggtcgaacct caggagctcg tcgggcgccg
     2041 aaccctcggt cacgagccgg gccaggtcga gccggtcagc tcaccgtgct cgacgcatcg
     2101 ggcggtccag ccgctcggga cgacctggac ggtcatccgg cgccggcagg cggggcagta
     2161 gcggggcggc tccaggctca gggcgccgag gcagcgctca tggtgccctg atgtggccgc
     2221 tccgccgcac cgcccgcagt agggcccccc ggatgtctcg ctcgcgtccg gactcgacgc
     2281 tggcgcgccg tccccggtca cagcgtggcg ttgagagcct tgatcggcat ctgcaggtcg
     2341 gccagcagcg acaagtcctc cttggggtca cggccgagcg tcgtcaggta gttgccgacg
     2401 atgacggcgt tgatcccgcc gagcagcccg tcgcgggtgc cgaggtcacc gagggtgagc
     2461 tcgcggccgc ccgcatagcg caggatcgtc cgcggcaacg cgagccggaa ggccgcgatg
     2521 caccgcaggg cgtcgttgct ctccatgatc ggcaggtcgg cgaacggcgt ccccgggcgg
     2581 gggttgagga agttgagcgg cacttcgtgg ggttcgagct ccccaagctg gtcggccagc
     2641 tcggcccgct gttcaagggt ctcgcccatg ccgacgaggc cgccgcagca cagctccatg
     2701 ccggagtcct tgaccatctg gcaggtgtcc cagcgctcct gccaggagtg cgtggtgacg
     2761 acgtggggga agtatgacgc ggccgcctcc aggttgtggt tgtagcggtg gatgcccatc
     2821 tcgacgagct cgccgacctg gtcctgcgtc agcatgccga tcgacgcggc gatgtggatg
     2881 tcgacctcgg ccttgatggc ggcgacgccc tcgcgcatct gggccatgag cctggcgtcg
     2941 ggcccgcgca cggccgcgac gatgcagaac tcgctcgccc cggtctcccg ggtcgacctg
     3001 gcggcgcgga cgagctcggg gatgttgagc cacaccgagc gcaccggcga ggtgaactgg
     3061 ccggactggc tgcagaagtg gcagtcctcg gggcagccgc ccgtcttgag gctgacgatg
     3121 ccctcgacct cgacgtcggg cccgttgtag gccatccgta cggcgtgggc cagcgccagc
     3181 agctcttcga gctggtcgtc cccggttcga aggacctcga ggatctggtc gtactgcagt
     3241 cgctcgcctc gctcgaggac ctgccctcgg gccaccccga ggatgccgtc gctgccgggg
     3301 gtgctggcgc ccgccgagct ctcggggccg ttgatgttgg gaaccgcgtt gtgaaccgcc
     3361 tgagccgtca tgagcgccat tcttgcgcat acgctcgtcc tcggctcccg ggtgtcattg
     3421 cgggacggtg tcatagcggg acggcgtcat ccagcgagca gccttcgtag cgtccgagga
     3481 gcgccggggt cgtcgacgac gaacatgagc gcgctgaggc ggccctcctc accgcgggcg
     3541 acgtccccca cgacgcgggg gttggtcagc cggagctccg ccaggaccgc cagcacgtcg
     3601 tcccgggcat ggaggaggtg ttgcccgatc gggccggtca aggtcgagga gccgtgcgtc
     3661 gtgcgccgga gctggcagga cagctcgaac cccagggcac cgaccagccg ctgcagcgcc
     3721 ccgatcgtgg ggaccttggc gccgcactcg tacgccgcaa ccacactctg gggcacaccg
     3781 gcgcgcgcgg cgagctcacc ctgggacacc gcttcgcgga accgggcttc ccgcaggatc
     3841 tcgcgcgcgg tcttggccgc gccggctcgg tggctccgca acgaccggcg ggcggggcct
     3901 ggatcgggtg tggcgacggg atcgggcttc gccccgcgat tctcgccgct cggcggctca
     3961 ggtgttctca caacggcact atcgcccctg agcctcgact cgtcacccgg tcctggcatt
     4021 cctgtggaca ggtgcccgag agcgctcggg ggtgtggacg gcgacctcgc ccacctgccc
     4081 tgctgccgcg gagcccccga gctgatgggg tgccggattc gagtcgatcg ccgaaaggcg
     4141 attccctgtc acttgtgcac cacggttcac agggcggcgc caccgtgcgg ccgcgcggcg
     4201 gtgggtgggg gacgggtcga gggagagccc ggcgtctggc ccgggccgag gaggtccgtc
     4261 ctgaggaggt ccgtcctgag g
//
LOCUS       DATAIX010000626         2461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_227300, whole genome shotgun sequence.
ACCESSION   DATAIX010000626 DATAIX010000000
VERSION     DATAIX010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227300"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..997)
                     /locus_tag="VHM65_09265"
     CDS             complement(35..997)
                     /locus_tag="VHM65_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2705935.1"
                     /translation="MSATARVSAEPVLRCSGLRKAFGQRVAVDGIGFEIAAGETYGLL
                     GPNGAGKTTTISMVCGLLTRDAGEVHVAGATVDVGATDAKAAIGYVPQDLAIYPDLTA
                     RENLRFFGLLQRMRGRELARRIDETLELIGLADRAKDRTEEFSGGMKRRLNIGIGLLH
                     GPKLLVLDEPTVGVDPQSRNAILASIEDLETEGMAILYTTHYMEEAERLCNRVGIIDD
                     GRICAEGTRRELIALVGQRDRVRLTASGDTRRAAEAVTEVASVERATVTDDGIDIVVS
                     DARAVLPALLADVTAAGASIRSVEVIEPDLEAVFLHLTGKALRD"
     gene            complement(1176..2189)
                     /locus_tag="VHM65_09270"
     CDS             complement(1176..2189)
                     /locus_tag="VHM65_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2705936.1"
                     /translation="MTLAIRPLDVHDDADCARAYEIFRAATAFERPHASTPSLAERVA
                     EWRHEDPAERMEMAVAVQGTEIVGVGEAFFPLADNTFMVWANVGVEPHRRRQGIGGAL
                     MAHVVERTRSEGRSSVLVEVTYPPDRHDDHPYRRFLEKHGFTLSNLEVMRRLELPVDE
                     ALLSELESQARARADGYVIETYVDGVPEHRQASLCAAMNRLGVDAPTGDVDFEEETLT
                     PERYQHFMGLLRQQGRHRLTAIAVHEASEQVSAYTDLVLPVGSPTHAWQWGTLVHGDH
                     RGHRLGMAVKVANLRLLQEHFPERREVFTGNADTNPWMISINERLGFRVIELCPAFQR
                     KLG"
     gene            complement(2233..>2461)
                     /locus_tag="VHM65_09275"
     CDS             complement(2233..>2461)
                     /locus_tag="VHM65_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705937.1"
                     /translation="GRGSGDELSVVAVVTRLGLGLDRLGGWGEHTLALPSGLWRDVLM
                     GREVRTDSAGARLADVLLDLPVALLVRVSGQ"
BASE COUNT          352 a          879 c          866 g          364 t
ORIGIN      
        1 cggcgatggc gagggcgggg tgcatgcggc ccactcagtc cctgagcgcc ttgccggtca
       61 ggtgcaggaa gacggcctcc aggtccggct cgatgacctc gaccgagcgg atcgaggctc
      121 ccgcagcagt gacgtcggca agcagcgccg gcaggacagc gcgggcgtcg gagacgacga
      181 tgtcgatccc gtcgtcggtc accgtggcgc gctcgacgct ggccacctcc gtgaccgcct
      241 ccgccgcccg gcgggtgtcg ccggaggccg tgagccggac ccggtcacgc tgaccgacca
      301 gggcgatgag ctctcggcgc gttccctccg cgcagatccg cccgtcgtcg atgatcccga
      361 cccggttgca caggcgctcc gcttcctcca tgtagtgcgt ggtgtagaga atcgccatgc
      421 cctcggtctc gaggtcctcg atcgaggcca ggatggcgtt gcggctctgc gggtccacgc
      481 ccaccgtggg ctcgtcgagg acgagcagct tgggcccgtg cagcaggccg atcccgatgt
      541 tgagccggcg cttcatcccg ccggagaact cctcggtccg gtccttggcc cggtcggcca
      601 ggccgatcag ctcgagcgtc tcgtcgatcc gccgggcgag ctcgcgcccc cgcatccgct
      661 gaagcaggcc gaagaaacgc aggttctcgc gggccgtgag gtccgggtag atcgcgaggt
      721 cctgtggcac gtagccgatc gccgccttcg cgtcggtggc gccgacgtcg accgtcgctc
      781 cagcgacatg gacctctccg gcgtcccgcg tcaggaggcc gcacaccatg gagatcgtgg
      841 tcgtcttgcc ggccccgttc gggccgagca ggccgtacgt ctcaccggca gcgatctcga
      901 acccgatccc gtccacggcg acccgctgac cgaaggcctt gcggagcccg ctgcaccgca
      961 ggaccggctc ggcagatacg cgcgcggtgg cggacatgag atctacctcg gcgcgtgcag
     1021 tggccggatc gacatggtcg ctgctccttc aggactgcac ccgtcgtgcc ccgctggtcg
     1081 cctccgggca ggtgcgggga gatcccactg cacgctacgc cgctgtgttc gggccccgca
     1141 agcgcacggc gaggggcgtg cgggcaccgg gcgggtcagc ccagcttgcg ctggaacgcc
     1201 gggcagagct cgatgacccg gaaccccagg cgctcgttga tcgagatcat ccacgggttg
     1261 gtgtcggcgt tgcccgtgaa gacctcccgg cgctcgggga agtgctcctg cagcaggcgc
     1321 aggttggcga ccttgaccgc cataccgagt cggtgtcctc gatggtcacc gtggacgagc
     1381 gtcccccact gccaggcatg ggtgggcgac ccgacgggca ggacgaggtc ggtgtaggcg
     1441 ctgacctgct cagacgcctc gtgcacggcg atggcggtca gccggtggcg cccctgctgc
     1501 cggagcagcc ccatgaagtg ctggtagcgc tccggcgtga gcgtctcctc ctcgaagtcg
     1561 acgtcaccgg tcggggcatc gaccccgagc cggttcatcg cggcacagag cgaggcctgc
     1621 cgatgctcgg gaaccccgtc gacatacgtc tcgatgacat acccgtccgc gcgggcccgt
     1681 gcctgggact cgagctcgga caggagcgcc tcgtcgaccg gcagctccag ccggcgcatg
     1741 acctcgaggt tgctcagggt aaaaccgtgc ttctcgagga accggcggta ggggtggtcg
     1801 tcgtgccggt ccggtgggta ggtgacctcg acgaggacgc tgctgcgccc ttcgctcctc
     1861 gtgcgctcga ccacatgagc catcagggcc ccaccgatcc cctggcggcg gcggtggggc
     1921 tcgacaccga cgttcgccca gaccatgaag gtgttgtccg ccagggggaa gaaggcctcg
     1981 cccacaccga cgatctccgt gccctgcacc gccacggcca tctccatccg ctcggccggg
     2041 tcctcgtggc gccactcggc gacccgctcg gccaggctcg gtgtcgacgc atggggacgc
     2101 tcgaacgccg ttgccgcccg gaagatctcg taggcgcgcg cgcagtcggc gtcgtcgtgg
     2161 acgtcgaggg gcctgatggc aagggtcacg acaccgaggg taaccagcgg agccgctcag
     2221 ggacgagggg ttttattggc cgctcacccg cacgagcagc gccaccggga ggtccaggag
     2281 cacgtccgcg agccgggcac cggcgctgtc cgtgcggacc tcgcgcccca tcagcacatc
     2341 gcgccacagc cccgacggca gggccagggt gtgctcgccc cagccgccga gccggtccag
     2401 ccctaggccg agccgggtga cgacggccac gaccgagagc tcgtcgccgc tgccgcgccc
     2461 g
//
LOCUS       DATAIX010000627         1768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_136019, whole genome shotgun sequence.
ACCESSION   DATAIX010000627 DATAIX010000000
VERSION     DATAIX010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136019"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VHM65_09280"
     CDS             <1..371
                     /locus_tag="VHM65_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX2705938.1"
                     /translation="PTVREPDGLAMSSRNLYLTASDRETALCLSGALLAGAEAATEGP
                     SAIRRAARSVLVREPLALIDYLVLVHPDTLEDVPEWYHGEALLAVAGRVGTTRLIDNL
                     PIDVGPGGGALEVSTTASRV"
     gene            368..>1768
                     /locus_tag="VHM65_09285"
     CDS             368..>1768
                     /locus_tag="VHM65_09285"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF005867.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEX2705939.1"
                     /translation="MTSAAPRPPVTVPRWLRAPAPGWTTTADVIVVGSGIAGLSIALQ
                     IRERVERVLLVTKTVLSAGSTAWAQGGIAAALAPGDSAEDHLRDTLVAGAGLCDIEAV
                     RALVGEGPARVRQLAALGARFDRDASGELSLAREGGHHRDRILHAGGDATGKEISRAL
                     IAALDHVRDDPGIEVIEHALVVDLLQDDAGRVCGVTLHVLGEGQIDGVGAAHARAVVL
                     ATGGLGQVYTATTNPAVATGDGLAAALRAGAVLADLEFIQFHPTVLWLGEGAHGQQPL
                     ISEAVRGEGAVLVDAAGVRFMKGVHELADLAPRDVVARAIVARMRQQGVDHVYLDCRH
                     LGRELLQARFPSIMARCRELGFDPATTPLPVAPAQHYASGGIWTDLEGRSCLNGLYAC
                     GEVSCTGVHGANRLASNSLLEGLVFAHRIADDVTARISAGELRMPHPNPASGASGHDP
                     VLPGSRRVELQRAMTAG"
BASE COUNT          216 a          679 c          637 g          236 t
ORIGIN      
        1 tgccgaccgt gcgcgaaccc gacggactcg cgatgagcag ccgcaacctc tacctcacgg
       61 cgtccgaccg cgagaccgcc ttgtgcctgt ccggagccct gctcgcgggt gccgaggcgg
      121 cgaccgaagg cccgtccgcg atccgccggg ccgcgcgcag cgtcctcgtc cgggagccgc
      181 tcgcgctcat cgactacctc gtgctcgtcc accctgacac cctggaggac gtccccgagt
      241 ggtatcacgg cgaggccctg ctcgccgtgg ctggccgggt cgggacgacc cggctcatcg
      301 acaacctgcc gatcgacgtc ggacccggtg ggggcgcgct cgaggtctcc accacggcga
      361 gccgggtatg acgagcgctg cgccccgccc gccggtcacc gtcccgcggt ggttgcgggc
      421 gcccgccccg ggctggacga cgacggcgga cgtcatcgtc gtcgggtcgg ggattgccgg
      481 tctgtcgatc gcgctgcaga tccgcgagcg ggtcgagcgg gtcctgctcg tgaccaagac
      541 ggtgctgtcg gccgggtcca ccgcctgggc gcagggcggg attgccgccg ccctcgctcc
      601 gggtgactcg gccgaggacc atctgcgcga caccctcgtc gcgggcgccg gcctctgcga
      661 catcgaggcg gtgcgcgccc tggtgggcga gggaccggcc cgggtccgcc agctcgcggc
      721 cctcggcgcc cgcttcgacc gggacgcctc gggcgagctg agcctggccc gcgagggtgg
      781 acatcaccgc gaccgcatcc tgcacgccgg tggcgacgcg accggcaagg agatctcgcg
      841 ggcgctcatc gccgccctcg accacgtcag ggacgacccg ggcatcgagg tcatcgagca
      901 tgcgctcgtc gtcgacctgc tccaggacga cgcgggccgg gtgtgcgggg tgaccctgca
      961 cgtcctcggc gaaggccaga tcgacggtgt cggcgcggcc cacgctcgcg cggtcgtcct
     1021 ggcgacgggc ggcctggggc aggtctacac agccacgacg aacccggcgg tcgcgaccgg
     1081 agacggcttg gccgctgcgc tgcgggccgg cgccgtcctg gcggacctcg agttcatcca
     1141 gttccacccc accgtgctgt ggctcggcga aggcgcacac ggccagcagc ccctcatctc
     1201 cgaggccgtg cgcggcgagg gggccgtcct cgtcgacgcc gccggggtgc ggttcatgaa
     1261 gggcgtccac gagctcgccg acctcgcacc ccgcgatgtc gtcgcccggg cgatcgtcgc
     1321 ccggatgcgc cagcagggcg tcgaccacgt ctacctcgac tgccgccacc tcgggcgtga
     1381 gctcctgcag gcgcgcttcc cctcgatcat ggcccggtgc cgcgagctcg gcttcgaccc
     1441 ggcgacgaca cccctgcccg tcgccccggc tcagcactac gcgagcggcg ggatctggac
     1501 cgacctcgag gggcgctcct gcctcaacgg cctctacgcc tgcggggagg tgtcgtgcac
     1561 gggcgtccac ggcgccaacc ggctcgcctc gaactcgctg ctcgagggtc tcgtcttcgc
     1621 ccatcgcatc gccgacgacg tcacggcccg gatctccgcg ggcgagctac ggatgccgca
     1681 cccgaacccc gccagcggcg cgagcggcca cgacccggtg ctgccagggt cgcgccgggt
     1741 cgagctgcag cgggcgatga cggccggg
//
LOCUS       DATAIX010000628         3100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_287538, whole genome shotgun sequence.
ACCESSION   DATAIX010000628 DATAIX010000000
VERSION     DATAIX010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287538"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VHM65_09290"
     CDS             <1..589
                     /locus_tag="VHM65_09290"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX2705940.1"
                     /translation="DLKGFTRLIDAVGGLEVDVKRRTPIGGETSKVSGYIEPGRQVLD
                     GYHALWYARSRAGSDNYERMARQRCVMDAMVHQLSPTTVVTKFQGIAAAGSEVVKTDI
                     PESELGTLADLGLKAKGQKITSVNFVPPLMKPWNYDPDFVREKVASTLEASAAAPASP
                     APSASASGPGSPTTSAPAATSGAAATDDLSVVCST"
     gene            589..1587
                     /locus_tag="VHM65_09295"
     CDS             589..1587
                     /locus_tag="VHM65_09295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2705941.1"
                     /translation="MTASSSPREPAAPYQPVSVVIPVLNEERHLRAAVRGILAQDYPG
                     ELEVVLAIGPCRDRTREVAAALCREDPRVRTVDNPSGRTPDALNAAIAASRHEIVARV
                     DGHAELSGGYLRLAVDELRRVGADNVGGIMDAQGVTPFERAVACAMRSRIGVGNAAFH
                     VGGEAGEAQTVYLGVFRREALARVGGYDPAFARAQDWEMNHRIRRTGGLVWFTPDLRV
                     TYRPRGSARALAGQYFHYGRWRRVVARHHDGTINLRYLAPPATVVATVVATVAGLFWR
                     PAWAVPAGYLLAVSIGGWLISRGETVPVRVRVPVAVTTMHWAWGIGFLTSPPSLAR"
     gene            1670..2269
                     /locus_tag="VHM65_09300"
     CDS             1670..2269
                     /locus_tag="VHM65_09300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX2705942.1"
                     /translation="MATRIQPSADVSPEAVIGDGSVVWHLAQVREGAVLGSGCIVGRG
                     AYVGTGVRMGDHCKLQNYALVYEPAVLEDGVFVGPAVVFTNDHFPRSVAPDGSLKRGD
                     DWEAVGVTCREGASIGARAVCVAPVTIGRWSLVAAGAVVIRDVPDFALVAGVPARRIG
                     WVGRAGVPLVQGDTAGVWRCPQTGATYREENECLTEADQ"
     gene            2266..>3100
                     /locus_tag="VHM65_09305"
     CDS             2266..>3100
                     /locus_tag="VHM65_09305"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX2705943.1"
                     /translation="MTAFIPAAKPLIGDEERAAVDRVLRSGMLAQGPEVKAFEEEFSA
                     TFGLGRACVAVSSGTSGLHLGLLSSGVRAGQEVIVPSFTFAATANSVALTGATPVFAD
                     IGADDFCLDPGAVEAAITGATVAIMPVHLYGHPARMTELMAVADRHGLQVFEDAAQAH
                     GASLDGRPVGAFGSFAMFSLYPTKNMTSGEGGMVSVATPEIERLMRLYRNQGMEKQYH
                     NEVVGFNARMTDIHAALGREQLKKVGGWTHTRQANAAFLSAHLDGVVTPPVAPGAEHV
                     YH"
BASE COUNT          434 a         1083 c         1135 g          448 t
ORIGIN      
        1 cgacctcaag ggcttcaccc gcctcatcga cgcggtcggc gggctcgagg tcgacgtcaa
       61 gcggaggacc ccgatcggtg gcgagacgtc caaggtgtcc gggtatatcg agccggggcg
      121 ccaggtgctc gacgggtacc acgccctgtg gtacgcgcgg tcccgggcgg gctcggacaa
      181 ctacgagcgg atggcgcgcc aacggtgcgt catggacgcg atggtgcacc agctcagccc
      241 cacgaccgtc gtgaccaagt tccagggcat cgccgccgcc ggctcagagg tcgtcaagac
      301 cgacatcccg gagtccgagc tgggcacgct agccgacctg gggctcaagg ccaagggcca
      361 gaagatcacg agcgtcaact tcgtcccgcc gctgatgaag ccgtggaact acgacccgga
      421 cttcgtgcgg gagaaggtcg cgtcgacgct cgaggcgtcg gcggcagcac cggcgagccc
      481 ggctccctcg gcgagtgcct cggggccggg ctcacccacg acctccgctc cggccgccac
      541 gagcggggcg gcagcaacgg acgacctgtc cgtggtgtgc tccacctgat gaccgcctcc
      601 tcgtcaccgc gcgagccggc cgcgccatac cagccggtca gtgtcgtcat accggtgctc
      661 aacgaggagc gccacctgcg ggccgcggtc cgggggatcc tggctcagga ctaccccggt
      721 gagctcgagg tcgtcctcgc gatcggcccg tgccgggacc gcacccgcga ggtggccgcg
      781 gcactgtgcc gcgaggaccc gcgggtccgg accgtcgaca acccgagcgg gcgcaccccg
      841 gacgcgctca acgcggccat cgccgcctcc cggcacgaga tcgtggcgcg cgtcgatggg
      901 catgccgagc tgtccggggg ctacctgcgt ctggcggtgg acgagctgcg tcgggtgggt
      961 gcggacaacg tcggcgggat catggatgcc cagggggtca cgccgttcga gcgagccgtc
     1021 gcctgcgcca tgcgcagccg gatcggcgtc ggcaacgcgg ccttccatgt cgggggcgag
     1081 gcgggcgagg cccagacggt ctatctcggg gtgtttcgcc gggaggcact ggcccgggtc
     1141 ggtggctacg acccggcgtt cgcccgagcg caggactggg agatgaacca ccggatccgg
     1201 cgcaccggcg gcctcgtgtg gttcaccccg gaccttcggg tcacctaccg tccccggggg
     1261 agcgcccgtg ccctggccgg ccagtacttc cactacgggc ggtggcgccg cgtggtggcc
     1321 cgccatcacg acggcacgat caacctgaga tacctcgcgc cgccggccac ggtcgtcgcc
     1381 acggtggtcg ccacggtggc aggcctgttc tggcgaccgg cgtgggccgt tcccgccggg
     1441 tatctgctcg ccgtctccat cgggggctgg ctcatcagcc ggggcgagac cgtcccggtc
     1501 cgagtccggg ttccggtggc agtcacgacg atgcactggg cctgggggat cgggttcctc
     1561 acgagcccgc cctcgctggc ccggtgatcg cggacggcgg ctcgagcccg ccctctggtg
     1621 gtcctgagct gccgcgagga cgggtgtctc tcaccaacga ggagggttag tggctacacg
     1681 aatccagccc agtgccgatg tgtcccccga ggctgtcatc ggcgacggct ccgtcgtctg
     1741 gcacctggcc caggtacgcg agggcgcagt cctcggcagc ggctgcatcg tcggccgggg
     1801 cgcctatgtg gggacgggtg tgcggatggg ggaccactgc aagctgcaga actatgcgct
     1861 cgtctacgag ccggccgtcc tcgaggacgg ggtcttcgtg gggcccgcgg tggtgttcac
     1921 caatgaccac ttccccaggt cggtggcgcc ggacggctcg ctcaagcggg gcgacgactg
     1981 ggaggcggtc ggcgtgacct gcagggaggg cgcctcgatc ggcgcccgcg ccgtgtgcgt
     2041 cgcgccagtc accatcggtc gctggtcgct ggtcgcggcc ggcgcggtgg tgatccgcga
     2101 cgttcccgac ttcgccctgg tggccggcgt tcccgccaga cggatagggt gggttggccg
     2161 cgccggggtc cctctcgtcc agggcgacac ggccggcgta tggcggtgcc cccagacggg
     2221 tgcgacctac cgcgaggaga acgagtgcct gacggaggcc gaccagtgac cgcattcatc
     2281 cccgcagcca agccgctcat cggcgacgag gagagggccg cggtcgaccg ggtcctgcgc
     2341 agcggcatgc tcgcccaggg acccgaggtc aaggcgttcg aagaggagtt ctcggccacg
     2401 ttcgggctcg ggcgtgcgtg cgtggcagtc agctcgggga cctcgggcct gcacctggga
     2461 ctgctgtcca gcggggtgcg cgccggccag gaggtcatcg tgccctcgtt cacgttcgcg
     2521 gcgacggcca actccgtggc cctgacgggc gcgacgccgg tgttcgccga catcggcgcc
     2581 gacgacttct gcctcgatcc gggggccgtc gaggccgcaa tcaccggcgc aaccgtcgcc
     2641 atcatgccgg tgcacctcta cggtcacccg gcgaggatga ccgagctgat ggccgtggcc
     2701 gaccgccacg gcctgcaggt cttcgaggac gcggcccagg cccacggggc ctcgctggac
     2761 gggcggcccg tgggggcctt cgggtccttc gcgatgttct ccctctaccc cacgaagaac
     2821 atgacgtcgg gcgagggtgg catggtctcg gtggcgacgc ccgagatcga gcggctcatg
     2881 cgcctctacc gcaaccaggg gatggagaag cagtaccaca acgaggtcgt cgggttcaac
     2941 gcccggatga ccgacatcca tgccgccctc ggccgcgaac agctcaagaa ggtcggcggc
     3001 tggacgcaca cgcggcaggc gaacgcggcc ttcctgtcgg cccacctcga cggtgtcgtg
     3061 acgcccccgg tggcacccgg cgcagaacac gtctaccacc
//
LOCUS       DATAIX010000629         2840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_227845, whole genome shotgun sequence.
ACCESSION   DATAIX010000629 DATAIX010000000
VERSION     DATAIX010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227845"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2649)
                     /locus_tag="VHM65_09310"
     CDS             complement(<1..2649)
                     /locus_tag="VHM65_09310"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX2705944.1"
                     /translation="MPSPLLQSARGTLELLEVDPAQAEREANRLLALAQAGGDDAAQS
                     VLERVLGRVCIRMADMGSAIEHCRRAVRAATRAGDDQLRGEARMTLALALNRTGDARR
                     ALREIERAIGDLDDAARPRALVQRATIHQELDSFDDALADYGEALPPLRRAGDLVWVQ
                     GLLMNRGVLYAFRHDYDAAVGDLREAAMLCEQLGLTHPLAAIEENLVLVYQRLGDIPA
                     ALTHFDRAERLAARLGTPTGSLMLERAELLLSVRLFDEAREAAELAVADFERRGRVLG
                     LPEARLVLARLTMIDARLDTAYEQARRAAAEFTRQRRQAWAALARYAALQIAAEDPAR
                     PTPPLSRMVAVADDLDREGWARAALDARMLAGRSWVASRGSARRVAQARGQLELVSAG
                     RHRGPAMGRTTAWHALALLRQGREDYPGASAAALAGLQILAEHRATMAATDLRARVSG
                     LGQDLAAIGLRNALARGRPFDVVVWAERGRAQHLLQRPVKAPDDPELARLLARLRRLT
                     SQLDEAESVGHKGPPANLQVQLERRIRDLSRRRHGAAVELTETCPTRAELRTLLGSAA
                     LVEYVQSDGLLHAVLLAGRRTTVVPVGSVAAVAGLVRWLPFALSRLARLTTRDQSKGS
                     AVAMLRHTAAALSASLVEPLRPLLGDRDLVIAPTGPLQFLPWSILPGLAGRAVTVTPS
                     AALWAAAIQREPRPGGVVVAAGPGLPGGDSEAIQIAALYGVSELRGADATVDRVGRAL
                     EGAAMAHLAAHGTVRADNPLFSALRMSDGPLTVFDLERLDHDADTVVLAACESGRSVV
                     LAGDELLGLSATLLAGDTRHLVASVVPVPDAETRPLMVALHERLLAGETVAAALAQAQ
                     AGIDEADPVAFAAAAGFICLGAGHGRP"
BASE COUNT          362 a         1058 c         1089 g          331 t
ORIGIN      
        1 cgggcgcccg tggccggcgc cgaggcagat gaacccggcc gcggcggcga acgcgaccgg
       61 gtcggcctcg tcgatgcccg cctgggcctg ggccagcgcc gccgcgaccg tctccccagc
      121 caggagccgc tcgtgcaggg ccaccatgag gggccgggtc tcggcgtcag gaaccgggac
      181 gaccgaggcg acgaggtggc gcgtgtcacc ggcgagcagg gtggcgctca ggccgagcag
      241 ctcgtcaccg gcgaggacga cgctgcggcc cgactcgcac gccgcgagga cgaccgtgtc
      301 ggcgtcgtgg tcgaggcgct cgaggtcgaa gacggtcagc ggcccgtcgg acatccgcaa
      361 cgcggagaag agcgggttgt ccgcccggac cgtgccgtgg gccgcgaggt gagccatcgc
      421 cgctccctcc agcgccctgc cgacccgatc gacggtcgcg tcggccccgc gcagctcgga
      481 gacgccatac agggcagcga tctggatcgc ctcgctgtcg ccgcccggca gccccggccc
      541 ggcggcgacg acgacgccac cgggcctcgg ctcacgctgg atcgcggctg cccacaaggc
      601 ggccgacggg gtgaccgtga ccgcccggcc ggccaggccc gggaggatcg accagggcag
      661 gaactgcagt gggccggtgg gtgcgatgac gaggtcacga tcgccgagga gcggccgcag
      721 tggctcgacg aggctcgcgc tcagggctgc cgccgtatgg cgcaacatgg cgaccgccga
      781 gcccttcgac tggtcgcggg tcgtcagccg ggccaggcgg gagagcgcga acggcagcca
      841 gcggacgagc ccggccaccg cggccacgga ccccaccggc acgaccgtgg tccgcctccc
      901 ggccaggagg acggcatgca ggaggccgtc gctctggacg tactcgacga gcgccgcaga
      961 cccgaggagc gtgcgcagct cggcccgggt cgggcacgtc tcggtgagct cgacggccgc
     1021 gccgtggcgc cgacggctga ggtcccggat gcggcgctcg agctggacct gcaggttcgc
     1081 cgggggtccc ttgtggccga cgctctcggc ctcgtcgagc tggctggtga gcctgcgcag
     1141 ccgggcgagc agccgggcca gctcggggtc gtcgggtgcc ttgaccgggc gctggagcag
     1201 gtgctgcgcc cgcccgcgtt cggcccagac gacgacgtcg aaggggcggc cccgggcgag
     1261 ggcgttgcgc aggccgatgg ccgcgaggtc ctggccgagc ccggagaccc gggctcgcag
     1321 gtcggtggcg gccatcgtcg cccgatgttc ggccaggatc tgcagcccgg cgagcgccgc
     1381 cgcggaggct cccgggtagt cctcgcggcc ctggcgcagc agggcgaggg catgccacgc
     1441 cgtcgtgcgg cccatggccg gcccacggtg gcgtcccgcg ctgacgagct cgagctgccc
     1501 gcgcgcctgg gcgacccggc gggcactgcc gcggctcgcg acccagctgc gtccggcgag
     1561 catccgggcg tcgagggcgg cgcgcgccca gccctcgcgg tcgaggtcgt cggccacggc
     1621 gaccatgcgg gacagcggcg gtgtcggccg ggccgggtcc tcggcggcga tctgcagggc
     1681 cgcgtaccgg gccagggccg cccaggcctg gcggcgttga cgggtgaact cggcagcggc
     1741 ccggcgcgcc tgctcgtaag ccgtgtcgag ccgggcgtcg atcatcgtga gccgggccag
     1801 gaccagccgg gcctcgggca gcccgagcac ccggccccgc cgctcgaagt ccgcgaccgc
     1861 cagctcggcg gcctcccggg cctcgtcgaa gagccgcacc gacagcagca gctcggcccg
     1921 ctccagcatg agcgagccgg tcggcgtccc cagccgggcc gcgagccgct cggcccggtc
     1981 gaagtgggtc agcgccgccg ggatgtcgcc gagacgttgg tagaccagga cgaggttctc
     2041 ctcgatcgcg gcgagcgggt gcgtcaggcc cagctgttcg cacagcatcg cggcctcccg
     2101 aaggtcgccc accgccgcgt cgtagtcgtg ccggaaggcg tagagcaccc cgcggttcat
     2161 caggagcccc tggacccaga cgaggtcgcc ggcccggcgc agcgggggaa gcgcctcgcc
     2221 atagtcggcc agcgcgtcgt cgaagctgtc cagctcctgg tggatcgtcg cccgctggac
     2281 cagcgctcgc ggcctggccg cgtcgtcgag atcgccgatg gcgcgctcga tctcgcgcag
     2341 cgcccggcgc gcgtcgcccg tccggttcag cgcgagcgcg agcgtcatcc gcgcctcacc
     2401 gcgcagctgg tcgtcgccgg cccgggtcgc cgcccgcacc gcccggcggc agtgctcgat
     2461 ggcgctgccc atgtcggcca tgcggatgca gacccggccg agcacccgct cgagcacact
     2521 ttgcgccgcg tcgtcgccgc cggcctgcgc cagagcgagg agacggttcg cctcccgctc
     2581 ggcctgcgcc gggtcgacct cgaggagctc gagggtgcct ctggcggact gcagcagagg
     2641 cgagggcacg ggaccactca atccgggccg ggcccacgtg tccagccagt agtctggcgc
     2701 cgacccgccg tgataggccg gcttcgatgt atcacggcac cccggccgcc ctccgtgttc
     2761 catgacggag agctattctc cgccctgttg cgtcgactgg agccccctga tgacagacct
     2821 gacgcaggac ccgcaggggc
//
LOCUS       DATAIX010000630         2779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_228043, whole genome shotgun sequence.
ACCESSION   DATAIX010000630 DATAIX010000000
VERSION     DATAIX010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228043"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VHM65_09315"
     CDS             <1..745
                     /locus_tag="VHM65_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX2705945.1"
                     /translation="SVTVTYRYVSTIFKAAVRDRRIAASPCDGIRLPKIIKSKVVPMS
                     TDEVRALCEAVPERYKAMIVLAAGTGMRQGEVFGLTLDRVDFLRRTLTVDRQLVGVQG
                     QAPKFGPPKTAASVRTIPLPQVVVMALAEHVRELPPRDDGPIFTGSGGQLLRRSAFGT
                     VWRRAAKTAGLKNHTFHSLRHYYASLLIRHGESVTTVQARLGHASAAETLDTYSHLWP
                     DSDDRTREAVDEVLGNPADSPRTSAASPW"
     gene            complement(770..1175)
                     /gene="rnpB"
                     /locus_tag="VHM65_09320"
     ncRNA           complement(770..1175)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VHM65_09320"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1211..>2779)
                     /locus_tag="VHM65_09325"
     CDS             complement(1211..>2779)
                     /locus_tag="VHM65_09325"
                     /inference="COORDINATES: protein motif:HMM:NF004114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEX2705946.1"
                     /translation="GAQTSYADLADQVSLAAEALHALGVRHGDRVALVLPNCPQHIVA
                     FYATLRLGAVVVEHNPLYTSGELEHQLADSGATVVVCWDKTAWVIDGLRATTAISTIL
                     AVDLSSALPWRKRLALRLPVAKARAARAAMTGPIPPGVQRWERFASSAGPIDPSYPEP
                     LAGDIALLQYTGGTTGTPKGAILTHENLRVNAAQGRAWVPGLRDGEEVVYAVLPLFHA
                     YGVTLCLTFSLSVGATLVLLPRFDLDQMLDAIRRLPPTFLPAVPPIYERVAAAARDRG
                     VDLTSIRYAISGAMPLPPEVVGIWESVTGGLLVEGYGMTETSPIVMGNPIAPTRRPGT
                     VGVPFPSTHIRIVDPDDPSQDLPLGVAGELLIWGPQVFQGYWNRPEESAAVLLPGGWL
                     RTGDIAVADVHGFVRIVDRIKELIITGGFNVYPSEVEDVLRALPEIADVAAVGVRSGG
                     DGGEEVVVAVVPAPGATVDTQRVRDFARERLTGYKVPRRVVIVEELPRSQVGKVLRRR
                     VRDRVEAMEALGPG"
BASE COUNT          412 a          995 c          965 g          407 t
ORIGIN      
        1 ctcggtgaca gtgacctacc gctatgtctc cacgatcttc aaagccgcgg tacgagatcg
       61 ccgcatcgcg gcgtcaccct gcgacggcat ccgacttccg aagatcatca agtccaaggt
      121 cgtcccgatg tccaccgacg aagttcgcgc gctctgcgag gcggtgcccg aacgctacaa
      181 ggccatgatc gtcttggcgg ccgggaccgg tatgcgccaa ggtgaggtct tcggcctgac
      241 gctggaccgg gtcgacttct tacgccggac gctcacggtt gaccggcagc tcgttggcgt
      301 gcaggggcag gcgccaaagt tcgggccgcc gaagacggca gccagcgtgc gcacgatccc
      361 gctcccacaa gtggtcgtca tggcactcgc cgagcacgtc cgggagctgc cgccgcgcga
      421 cgatgggccg atcttcaccg ggtcaggcgg tcagctcttg cgccggtctg ccttcggcac
      481 ggtctggcgc agggctgcta agactgctgg gttgaagaac cacaccttcc acagcctgcg
      541 gcactactac gcctcgctgc tcatccgcca cggcgagtcg gttacgacgg tgcaggcccg
      601 gctcgggcac gcgagcgcag ccgagacgct ggacacctac agccatctgt ggcccgactc
      661 ggacgaccgc acgcgggagg cagtcgacga ggttctcggc aatcctgcgg actccccgcg
      721 gacttccgcg gcgtcgccat ggtaaaactg caggtcaaaa ggcatttagc ggatgagtcg
      781 gtcgatacgc cgggttctgt cggccgcggt ccgttgccgg gccgtggcgg gcggtcatcc
      841 atctcgggtg cccgttgccg gacacctcta gcgctctacc cgtgtgctcg ggcgggccgc
      901 cctcggtcgc acactgtctg agcttgctcc aggtggggtt tgccgagccg gccgggtcac
      961 cccggccgct ggtggtctct tacaccaccg tttcaccctg accgcgacgc ctgccagccg
     1021 agccggtggt gcgcctgcgg cggtctgttt tctgtggcac tgtcccgcgg gtcgccccgg
     1081 gtggccgtta gccaccacct tgccctgtgg agcccggacg ttcctcggcg gagcggcgcc
     1141 gaagcaccgt ccgacgcgac cgcctgaccg actcatccac gacccaggat acggcccggc
     1201 ccgagtcccg tcagccaggg ccgagggcct ccatcgcctc gacccggtcg cggacccgtc
     1261 tccgcaggac cttgccgacc tgcgaacggg gcagctcctc gacgatgacg acccgccggg
     1321 gcaccttgta gccggtgagc cgttcccgcg cgaagtcccg cacccgctgg gtgtcgacgg
     1381 tggcaccggg cgccgggacg acggccacga cgacctcctc gccaccgtcc ccgccgctgc
     1441 ggacgcccac ggcggcgacg tcggcgatct cgggaagcgc gcgcaggacg tcctcgacct
     1501 cggacgggta gacgttgaat cccccggtga tgatgagctc cttgatgcgg tcgacgatcc
     1561 ggacgaagcc gtggacgtcc gcgacggcga tgtcaccggt gcgcagccac ccgcccggca
     1621 gcaggaccgc cgccgactcc tccggacggt tccagtagcc ctggaacacc tgcggccccc
     1681 agatgagcag ctcgccggcc accccgagcg gcaggtcctg ggacgggtcg tcggggtcga
     1741 cgatgcgaat atgggtcgag gggaagggca ccccgaccgt cccgggccgc cgggtcggcg
     1801 cgatcgggtt ccccatcacg atcggtgacg tctcggtcat cccgtagccc tcgacgagca
     1861 gcccgccggt gaccgactcc cagatgccga ccacctcagg cgggagcggc atcgccccgg
     1921 agatcgcgta gcggatggac gtgaggtcga cgccgcgatc gcgggccgcc gccgccaccc
     1981 gttcgtagat gggcgggacc gcgggcagga acgtcggggg aagccgccgg atcgcgtcga
     2041 gcatctggtc gaggtcgaac cggggcagca ggaccagcgt ggcccccacg ctcagggaga
     2101 acgtcaggca caaggtcacg ccatacgcgt ggaagagcgg cagcaccgca tagacgacct
     2161 cctcgccgtc ccgcaggccg ggcacccagg cccgtccctg ggccgcgttg acccgcaggt
     2221 tctcgtgggt gaggatcgcg cccttggggg tgccggtcgt cccccccgtg tactgcagca
     2281 gcgcgatgtc gccggcgagg ggctccgggt atgacgggtc gatcgggccg gcggacgacg
     2341 cgaagcgctc ccagcgctgg acgcccggtg ggatggggcc ggtcatcgcc gccctcgcgg
     2401 cacgcgcctt tgcgaccggc agcctcaggg cgaggcgctt gcgccacggc agcgcgctgg
     2461 acaggtcgac ggcgaggatt gtcgagatgg cggtcgtggc ccgcagcccg tcgatgaccc
     2521 acgccgtctt gtcccagcac acgacgaccg tggccccgct gtcggccagc tgatgctcga
     2581 gctcgcccga ggtgtagagc gggttgtgct cgacgacgac cgcgcccaga cgcagcgtgg
     2641 cgtagaaggc gacgatgtgc tgcgggcagt tgggcaggac cagcgccacc cggtcaccgt
     2701 gccggacgcc cagggcgtgc aaggcctccg ccgccaggct cacctggtcg gccagatccg
     2761 cgtatgacgt ctgcgcccc
//
LOCUS       DATAIX010000631         3321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_228152, whole genome shotgun sequence.
ACCESSION   DATAIX010000631 DATAIX010000000
VERSION     DATAIX010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228152"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            400..1041
                     /locus_tag="VHM65_09330"
     CDS             400..1041
                     /locus_tag="VHM65_09330"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX2705947.1"
                     /translation="MRAGPGGLQELWTARLTVRDVLLCRDDPRLEVVATSSGRYDARL
                     SVALAGRLVEFPRAFVSADLAVEGAEVRVVGTHLDSLDPAVALAQARELVTGPAGRSG
                     AVVVLGDLNSDPCDEATAAAYRHMLTHGFTDCWPAWSPDQPGFTCGLGELLDDETPAG
                     LDRRLDVVLARSGTEAPIRVTGAWLTGVDMADRDPATGLWASDHAGVVATLWL"
     gene            1095..2288
                     /locus_tag="VHM65_09335"
     CDS             1095..2288
                     /locus_tag="VHM65_09335"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEX2705948.1"
                     /translation="MPRFEPFPALRYAPGVDLAAVTAPPYDVLSGADVDALQDRDPRN
                     ITHVDVPRGGSSRYDVAGHLLRQWMSTGVLVADAAPSLTVYRMRFTDATGARRELVGV
                     LGAVEVVDEGAGGVLPHERTTPKASTDRLDLTRATEANLSPVWGLSLAHGLSALLQEP
                     GEPVGSVTAGGVEHVVERVADPGRTALICARIGSDDVLIADGHHRYGVGRHYRDEVRA
                     RSGRHDTPAELTLTYVAELTPDQLNIDAIHRVYRGVSHADLRAALGRSFDLSPAPAPT
                     PDLLVAMARLGRLVLVGPSGPAEWLVPKAGVLQDVRALDGAWLEHALRGSAAEVSYQH
                     GLPGVLDAVGSGAAAVLIRPVSITEIERTAREGLLMPPKSTFFTPKPLTGLVVRDLRE
                     GPSQS"
     gene            complement(2224..2886)
                     /gene="idi"
                     /locus_tag="VHM65_09340"
     CDS             complement(2224..2886)
                     /gene="idi"
                     /locus_tag="VHM65_09340"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEX2705949.1"
                     /translation="MSVEELVVLVDEDGRAIGTAPKAQVHHGATPLHLAFSCYLYWGE
                     DVLVTRRALTKATFPGVWTNSVCGHPGPGEPIESAVTRRARDELGVEVTRIQLVLPRF
                     RYWAEAGGVVENELCPVFAGWVADASTTLAPAEVDAAHWEPWCPFRDDVVSGVREVSR
                     WCAEQVRELTALGDHPGQWPTADPALLPHAIRRTPRDTRPSSGTGLRAGREPPGRSTA
                     SA"
     gene            complement(2883..>3321)
                     /locus_tag="VHM65_09345"
     CDS             complement(2883..>3321)
                     /locus_tag="VHM65_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705950.1"
                     /translation="YEVLLRDFPTLRERLGRAGTITSVRGAGPLRQRTVRRVAGRVLL
                     AGDASGYVDALTGEGIALGMAHALAAVDAVAAARPLGYERSWRQATWRYALMTHALVQ
                     ATRFGPARRALVPAASALPGVFAAAVNELARPAAQTDRDGARR"
BASE COUNT          415 a         1218 c         1226 g          462 t
ORIGIN      
        1 caccaggggt cttcggccgc tgaccgtgat gacgcgcaac ctctatctcg ggtgtgatat
       61 cgcacgggtc atcggcgccg ccggtagcac cgggcagggc gccgcgaccg cccaggctgt
      121 cgcgaacggc gctctgcgtc ataccgtttc actcacccgc ttcgacgtcc gcagcgggct
      181 gctcgctcgg gagatcgccg cgcgctgtcc tgacgtcgtc gccctgcagg aggtggcgct
      241 gtggcggcgc ggccgctggg accccggtgc tcccggcacc ggcgcggcgg ccgaggtcga
      301 ggaggacttc ctcgacctgc tgcagcgcgc cctggaccgg gagcgggcgc cataccgggt
      361 cgctgtgccc aggacggcgc cgacgtccag tccccagcgg tgcgagccgg gccgggtggc
      421 ctgcaggagc tctggacggc gcgcctcacg gtgcgcgacg tcctcctctg ccgcgacgac
      481 cctcgcctcg aggtggtggc gacgagcagc ggccggtatg acgcgcgcct gtcggtggcg
      541 ctcgccggac gcctggtgga gttcccccgc gcgttcgtct cggcggacct cgccgtcgag
      601 ggcgccgagg tccgggtcgt cgggacccat ctggactcac tcgacccggc agtcgcgctg
      661 gcccaggcac gcgagctcgt cacggggccg gccggccggt ccggagccgt cgtcgttctc
      721 ggggacctca actccgaccc ctgcgacgag gcgacggccg cggcctaccg gcacatgctg
      781 acccacgggt tcacggactg ctggccggcg tggagcccgg atcagccagg cttcacctgc
      841 ggcctcgggg agctgctcga cgacgagacc ccggcaggct tggaccggcg cctcgacgtc
      901 gtcctggccc gctcggggac cgaggcaccc atccgggtga ccggtgcgtg gctcaccgga
      961 gtggacatgg ccgaccgcga tcccgctacc gggctgtggg cctcggacca cgccggagtg
     1021 gtggcgacgc tctggttgtg accgcccata agtggccgtc cgctcgcggc cccctctcgt
     1081 ggcaggctgg gagcgtgcct cgcttcgaac cgttcccggc gctgcgctac gccccgggcg
     1141 tcgacctcgc cgcggtgacc gcgccgccct acgacgtcct ctccggtgct gacgtcgacg
     1201 cgctgcagga tcgcgacccg cgcaacatca cgcacgtcga cgtcccccga ggcggttcga
     1261 gccggtatga cgtcgccggt cacctcctgc ggcagtggat gtcgaccggt gtgctcgtcg
     1321 ccgacgcggc gccgtcactg accgtctacc gcatgcggtt cacggacgcg acgggggcgc
     1381 ggcgcgagct cgtcggggtc ctcggcgcgg tggaggtggt cgacgagggg gccggcggtg
     1441 tcctgccgca tgagcggacc acgccgaagg cctcgaccga ccggctcgac ctcacccgcg
     1501 cgaccgaggc gaacctctct cccgtctggg gtctgtcgct cgctcatgga ctgagcgccc
     1561 tgctccagga gccgggtgag ccggtcgggt cggtcaccgc agggggcgtc gagcacgtcg
     1621 tcgagcgggt cgccgatccg ggccggaccg ccttgatctg cgccaggatc ggctccgacg
     1681 acgtactcat cgccgacggc catcatcggt atggcgtcgg ccggcactac cgcgacgagg
     1741 tccgggcccg cagcgggcgc cacgacacgc cggccgagct gaccctgacc tacgtcgccg
     1801 agctgacccc cgaccagctc aacatcgacg cgatccaccg tgtctaccgg ggggtctcgc
     1861 acgcggacct gcgggccgcg ctggggcggt ccttcgacct gtccccggct ccggcgccga
     1921 cgccggacct gctcgttgcg atggcccggc tcgggcggct cgtgctcgtc gggccctcgg
     1981 gaccggccga gtggctcgtg ccgaaggcgg gtgtcctgca ggatgtgcgg gcgctcgacg
     2041 gggcctggct cgagcacgcg ctgcgcggct cggcggccga ggtgtcctac cagcacgggc
     2101 tgcccggcgt gctcgatgcg gtgggctcgg gtgcggcggc ggtgctcatc cggccggtga
     2161 gcatcaccga gatcgagcgc accgcccgcg agggcttgct catgcccccg aagtcgacct
     2221 tcttcacgcc gaagccgttg accggcctgg tggttcgcga cctgcgcgaa ggcccgtccc
     2281 agagctagga cgggtgtccc tcggcgtccg ccggatcgca tgcgggagca gggccggatc
     2341 ggcggtcggc cattggccgg ggtggtcgcc gagggcggtg agctcgcgga cctgctcggc
     2401 gcaccatcgg gacacctccc ggaccccgga cacgacgtcg tcgcggaacg ggcaccacgg
     2461 ctcccagtgc gcggcgtcca cctcagctgg tgccagggta gtcgaggcgt cggcgaccca
     2521 gccggcgaag acggggcaga gctcgttctc gacgactcca ccggcctcgg cccagtagcg
     2581 aaagcgaggc aagaccagct gtattcgcgt cacctcgacc ccgagctcgt cccgggcgcg
     2641 ccgggtcacc gccgactcga tcggttcacc gggcccggga tgcccgcaca cggagttggt
     2701 ccagaccccc gggaaggtcg ccttcgtcag cgcccgccgg gtgaccagga catcctcgcc
     2761 ccagtagagg tagcacgaga acgccaggtg cagcggggtg gcgccgtgat ggacctgcgc
     2821 cttcggcgcg gtgccgatcg ctcgaccgtc ctcgtcgacg aggacgacga gctcctcgac
     2881 gctcatctgc gcgctccgtc gcgatcggtc tgcgctgcgg gccgggcgag ctcgttgacc
     2941 gcagcggcga agacgcccgg gagcgccgag gcggccggga cgagtgcccg ccgggccggt
     3001 ccgaagcggg tggcctgcac gagggcatga gtcatgaggg cgtacctcca ggtcgcctgc
     3061 cgccacgacc gctcgtagcc gagagggcgc gccgccgcga ccgcgtcgac ggcggccagc
     3121 gcatgcgcca taccgagcgc aatgccctcg ccggtgagcg cgtcgacata cccgctggca
     3181 tctccggcga gcaggacccg tcccgcgacc cggcgcacgg tgcgctggcg cagggggccg
     3241 gcgccgcgga ctgaggtgat ggtgccggcg cggccgagcc gctcgcgcag cgtcgggaag
     3301 tcgcggagca ggacctcgta t
//
LOCUS       DATAIX010000632         1904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_136433, whole genome shotgun sequence.
ACCESSION   DATAIX010000632 DATAIX010000000
VERSION     DATAIX010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136433"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /gene="whiA"
                     /locus_tag="VHM65_09350"
     CDS             complement(<1..304)
                     /gene="whiA"
                     /locus_tag="VHM65_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEX2705951.1"
                     /translation="MAMTARVKDELSRLDVTKPCCRKAEVSATLRFAGGLHIVGGRIV
                     VEAELDTANAARRLRRLIAEVYGHHSDVVVMSAGGLRRGSRYVVRVVDEGATLARQT"
     gene            complement(386..1327)
                     /gene="yvcK"
                     /locus_tag="VHM65_09355"
     CDS             complement(386..1327)
                     /gene="yvcK"
                     /locus_tag="VHM65_09355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HEX2705952.1"
                     /translation="MSGPRVVAMGGGHGLAASLSALRLVTDHITAVVTVADDGGSSGR
                     LRGEFHVLPPGDLRMALAALCDDSAWGHTWRDVLQHRFQGDGQLAGHALGNLLIISLW
                     ELLGDTVEGLDLVGRLLNARGRVLPMAAEPLEIVAEIRGLDRDRPDEVTEVRGQHLVA
                     SADGLVESVRIVPAQPRSCPQTLAAVTDADWVFLGPGSWFTSVIPHVLVPELREALVA
                     TSAQRVLILNLEVGRGETAGYRVHDQLRSLADHAPELAIDVVLVDPAVVDDLGRLSAA
                     AAELGATLSVQPVAAVGRRATHDSLRLAAAYRDLMRA"
     gene            complement(1331..>1904)
                     /locus_tag="VHM65_09360"
     CDS             complement(1331..>1904)
                     /locus_tag="VHM65_09360"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX2705953.1"
                     /translation="LVRRFESVRRPHPLQGEGRLLDGIVREREMLVGLRSDADMIIDT
                     SGLNVHALTAKITSIFAQPTAAQIRIAVMSFGFKYGVPLDADFVFDMRFLPNPFWDPE
                     LRPFTGLDVAVADFVLGQPGAMRFIDGVVATLEPVTEGYIREGRRYVTIAVGCTGGKH
                     RSVAVAEALATRLAGPDVATFLVHRDLGRE"
BASE COUNT          311 a          655 c          701 g          237 t
ORIGIN      
        1 cggtctggcg ggccagcgtc gccccctcgt cgacgacgcg gacgacatac cggctccctc
       61 gccgcagccc acccgcggac atgaccacga cgtcgctgtg gtggccgtag acctcggcga
      121 tgaggcgccg caggcgccgg gccgcgttgg ccgtgtcgag ctcggcctcg acgacgatgc
      181 ggcccccgac gatgtgcagg ccaccggcga agcgcaacgt agccgacacc tccgccttgc
      241 ggcagcaggg cttcgtcacg tccagacggc tcagctcgtc cttgaccctg gccgtcatcg
      301 ccataccgaa catcctgaca tcgacatcat ctccccgtgt ccgggcatgc tacgcgactc
      361 cgggctccgg tgagaggtca agccgtcaag cacgcatgag atcgcggtat gccgccgcga
      421 ggcgcaggga gtcatgggtg gcccggcggc ccaccgcggc gaccggctgg acggacaggg
      481 tggctccgag ctcggccgcg gcggccgaca gccgcccgag gtcgtcgacg actgcggggt
      541 cgacgagcac gacgtcgatg gcgagctcag gggcatggtc ggcgagggaa cgcagctggt
      601 cgtggacacg gtagcccgcc gtctcgcccc ggccgacctc gaggttgagg atgagcaccc
      661 gctgtgcgga ggtggcgacg agcgcctcgc gcagctcggg cacgaggacg tgcgggatga
      721 ccgaggtgaa ccacgagccg ggcccgagga agacccagtc cgcgtcggtg acggcggcga
      781 gagtctgcgg gcaggaacga ggctgggcgg ggacgatccg caccgactcg acgagaccgt
      841 cggccgaggc caccaggtgc tgaccccgca cctcggtcac ctcgtcggga cggtcccggt
      901 ccaggccgcg gatctcggcg acgatctcca acggctcggc cgccatcggc aggacccggc
      961 cgcgggcgtt gagcaggcgc ccgaccaggt cgagcccctc gacggtgtcg ccgagcagct
     1021 cccagaggga gatgatcagc aggttgccca gggcatggcc cgcgagctgg ccgtctccct
     1081 ggaaccggtg ctgcagtacg tcgcgccacg tgtggcccca ggccgagtcg tcgcacaggg
     1141 ccgccagagc catccgcagg tcgcccgggg gcagcacatg gaactcgccg cggagccggc
     1201 cgctggaccc gccgtcgtcg gccaccgtga ccacggccgt gatgtggtcg gtcacgagcc
     1261 gaagggccga cagggaggcg gccagaccgt gtccgccgcc catggccacg acccgggggc
     1321 cgctcatcgg ctactcacga ccgaggtcac ggtggacgag gaaggtggcg acatcgggcc
     1381 cagccagccg ggtggccagc gcttcggcca ccgcgaccga gcgatgcttg ccccctgtgc
     1441 atccgacggc gatcgtcaca tagcggcggc cctcgcgaat gtagccctcg gtgaccggct
     1501 ccagcgtggc aacgaccccg tcgatgaacc gcatggcgcc aggctggccg aggacgaagt
     1561 ccgccacggc gacgtccagc ccggtgaagg gtcgcagctc gggatcccag aacgggttgg
     1621 gcaggaagcg catgtcgaag acgaagtcgg cgtcgagggg gacgccgtac ttgaagccga
     1681 aggacatgac ggcgatgcgg atctgagcgg ccgtgggctg ggcgaagatc gaggtgattt
     1741 tagcggtgag agcgtggacg ttcagacccg aggtgtcgat gatcatgtcg gcgtccgagc
     1801 gcagcccgac gagcatctcg cgctcgcgga cgataccgtc gagcaggcgg ccctccccct
     1861 gcagcgggtg gggacggcgg acgctctcga accggcggac cagc
//
LOCUS       DATAIX010000633         2041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_288652, whole genome shotgun sequence.
ACCESSION   DATAIX010000633 DATAIX010000000
VERSION     DATAIX010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288652"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..968)
                     /locus_tag="VHM65_09365"
     CDS             complement(192..968)
                     /locus_tag="VHM65_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2705954.1"
                     /translation="MTTRATCVLAPNPGVMTLDGTNTWVLREPGADQVVVVDPGPLDG
                     GHLAAVQAVVADTASSVALVLLTHRHADHAESAVRFAELTGAPVRALGVGHDDLAGGD
                     AVRVAGLDLVVVATPGHTSDSLSLVVPADAALLSGDTVLGRGTTVVAWPDGELGSYLD
                     SLERIGDLTGAGAVRTILPGHGPCVYDAAAMVRGYLTHRHARLDQVREARAAGARDVH
                     EVLERVYWDVPLEVWPAAELSVRAQLAYLDRPSGPAPESA"
     gene            complement(1018..1923)
                     /locus_tag="VHM65_09370"
     CDS             complement(1018..1923)
                     /locus_tag="VHM65_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705955.1"
                     /translation="MTRDFSLGDSSVLLERAERHLAGPSTDPVQPRLAATVILVRDAG
                     QGPVEVFLMWRTATMEFAPSTIVFPGGAVDRRDARPDLPWAGPSVTEWARRLRCDPVS
                     AQLLVAAAVRELFEESGVLLASRADAGPQARLAEVSDPSWDEMRVRLVEREVGLAEVL
                     ITHGLALRSDLLVAHAHWITPPFERRRYDTHFFAAAMPAGQEARHLSTEADEADWADP
                     ARVVADYAAGRAVLLPPTLVCLEELAAAESAAAYLAGPRQMVPIMPVLAREADGSVVL
                     RVAELGSAATADVAPRGGRPGVCGA"
BASE COUNT          287 a          770 c          765 g          219 t
ORIGIN      
        1 gcggcctcct cgtcgcccat gacctcaccc aggaggagct cgcccagctg gtcggggcct
       61 cccgcgagac ggtcaacaag gcgctggccg acttcgcgac ccgcggctgg ctcaagctcg
      121 aagcccgggc cgtgctcctc atcgacatcg accggctcaa ccgccgggcc cgctgagccc
      181 gcagagcccg actacgccga ctcaggcgct ggccccgatg gccggtcgag ataggccagc
      241 tgcgcccgca ccgacagctc ggccgccggc cacacctcca gcgggacgtc ccagtagacc
      301 cgctcgagca cctcgtgcac gtcgcgggcc ccggccgccc gggcctcgcg gacctggtcg
      361 agccgcgcgt gacgatgggt gaggtacccg cggaccatcg cggcggcgtc atacacgcag
      421 gggccgtggc cgggcaggat cgtgcgcacg gccccggcac cggtgaggtc cccgatccgc
      481 tcgagcgagt cgaggtagga gccgagttca ccgtcgggcc aggccacgac cgtcgtcccc
      541 cggcccagga ccgtgtcgcc ggagagcagc gccgcatcgg cggggacgac gaggctcagc
      601 gagtccgacg tgtgacctgg cgtggccacg acgacgaggt cgaggccggc cacccgcacc
      661 gcgtcaccgc cggcgaggtc gtcgtgtccc acgccgaggg ctcgcacggg cgccccggtc
      721 agctcggcga agcggactgc cgactcggca tggtcggcgt gccggtgggt gagcaggacg
      781 agcgctaccg agctggccgt gtccgcgacg accgcctgca cggcagccag atgccccccg
      841 tcgagcggcc ccgggtcgac gacgacgacc tggtcggctc ccggttcgcg caggacccag
      901 gtgttggtgc cgtcgagcgt catgactccc gggttcgggg cgagcacgca ggtcgcccgg
      961 gtggtcaccg gcccgctgcc ccactcaccg acgggcgcag tcggcgccgg tatgccgtca
     1021 cgcaccgcag acgcccggcc ggccgcccct gggggccacg tccgccgtcg cggcggaccc
     1081 cagctcggcc acccgcagga cgaccgagcc gtcggcctcc cgggccagca ccggcatgat
     1141 gggcaccatc tggcgcggcc cggcgaggta ggcagcggcc gactccgccg cggccagctc
     1201 ctcgaggcag acgagcgtcg gcgggagcag cacggcccgg ccggcggcgt agtcggcgac
     1261 cactcgcgcg gggtcggccc agtcggcctc gtcggcctcg gtactcaggt gccgcgcctc
     1321 ctggccggcc ggcatcgcgg ccgcgaagaa gtgggtgtcg tagcggcgcc gctcgaaggg
     1381 cggggtgatc cagtgggcgt gggcgacgag caggtcggac cgcaaggcga gcccgtgggt
     1441 gatgagcacc tcggccagtc cgacctcgcg ctcgacgagg cggacccgca tctcgtccca
     1501 cgaggggtcg gacacctccg cgaggcgcgc ctgcggaccc gcatccgcac ggctcgccag
     1561 caggaccccg ctctcctcga acagctctcg cacggcggcc gcgacgagca gctgggccga
     1621 gacggggtcg cagcggagcc gccgggccca ctcggtcacg gacgggccgg cccagggcag
     1681 gtcagggcgc gcgtcgcgcc ggtccaccgc accgccgggg aagacgatcg tgctcggggc
     1741 gaactccatc gtggccgtgc gccacatgag gaacacctcg accgggccct gacccgcgtc
     1801 tcgcacgagg atgacggtcg cggcaaggcg tggctgcacc gggtcggtcg aggggccggc
     1861 gaggtggcgc tcggcgcgct cgagcaggac cgaggagtca ccgagggaaa agtcgcgagt
     1921 cacaccccga tgctagccac cagatgccgc ggcgagccgg cgaccgcgag cgcctcgccg
     1981 ggggcaggct cagcatgccg tcgaggtgcg cggcgggaga tcgggcccgg ccgacacgct
     2041 g
//
LOCUS       DATAIX010000634         1561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_288942, whole genome shotgun sequence.
ACCESSION   DATAIX010000634 DATAIX010000000
VERSION     DATAIX010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288942"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VHM65_09375"
     CDS             complement(<1..787)
                     /locus_tag="VHM65_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2705956.1"
                     /translation="MSPTATVAPNGSGRPAMEPLDQVPPVIEFARLSRTYPGPPPVPA
                     LRPCDLVVRRGEHVAVVGPSGSGKSTLLNVLGLLDRPTAGTYHLDGIDVGDLPERDRT
                     ALRGRRIGFVFQAFHLLPHRDAVENVLVAMLYADPDRRTRRARAVAVLTSVGLGHRLT
                     VPTALLSGGERQRVAIARALVNRPSLLLCDEPTGNLDSSNSGTVLALLEDLHRQGITL
                     LVITHDAAVAGRAQRVVTIRDGELSETPRSPGSGSLALGRRRFM"
     gene            complement(784..>1561)
                     /locus_tag="VHM65_09380"
     CDS             complement(784..>1561)
                     /locus_tag="VHM65_09380"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX2705957.1"
                     /translation="VGTTTGGATGTASGTGAGSTSRSLQLRYARQDRDRALTALTAAR
                     AKAGPMVPASEVIFMRTLPARVVSMNAPLGRAVPTDMLVLSSGAMQVKAKLQPYQRAR
                     VKVGQKVQIFSDDTGKQYPGVVSSVGATPVSDSGGSGGSGDQGQGQGQGSSGEQGAGG
                     DEAALYYLVMIKPTKAIPASLQGLAVRVTVEKSASAGQVLAVPSAAVSSRSDGASIVT
                     VQDAAGALRTVVVTPGVSGDGYVEVSGGGIRAGDAVVVGQ"
BASE COUNT          221 a          533 c          593 g          214 t
ORIGIN      
        1 gcatgaacct tcgccgaccc agggccaggg acccggaccc gggagaccgt ggggtctcgc
       61 tcagctcgcc gtcgcggatg gtcacgacgc gctgggcgcg ccctgcgacc gcggcgtcgt
      121 gggtgatgac gagcagggtg atgccctgcc ggtgcaggtc ctccaggagc gcgaggacgg
      181 tcccgctgtt ggacgagtcg aggttcccgg tgggctcgtc gcagagcagc aggctgggcc
      241 ggttgaccag ggcgcgggcg atggcgacgc gctggcgctc accgccggag agcagcgcgg
      301 tgggcacggt gagccgatgg ccgagtccga cggaggtgag cacggcgacg gcgcgtgcgc
      361 gccgggtgcg ccggtcgggg tcggcgtaga gcatggcgac gaggacgttc tccacagcat
      421 cacgatgggg gagcagatgg aacgcctgga agacgaagcc gatccgccgg ccccgcagcg
      481 ccgtgcggtc gcgttcgggc aggtccccca cgtcgatgcc gtccagatgg taggtgccgg
      541 cggtcggacg gtcgagcagt ccgagcacgt tgagcagagt cgacttgccg gagccggagg
      601 gcccgacgac cgcgacatgc tcgccacggc ggacgacgag gtcacagggg cgcaacgcgg
      661 gcacgggggg cggcccgggg taagtccgcg agagccgggc gaactcgatg accggaggta
      721 cctggtccag cggctccatc gcaggtcggc cggagccgtt gggtgccacc gtggccgtcg
      781 ggctcattgt ccgaccacca ccgcgtcgcc cgcacggatc ccgccgccgg acacctcgac
      841 atacccgtcc ccgctcacgc ccggcgtgac gacgacggtg cgcagggccc ctgcggcgtc
      901 ctggacggtg acgatgctgg cgccgtcgga gcgtgaggac accgccgcgg agggcacggc
      961 cagcacctga cccgccgagg cagacttctc gacggtgacc cggacggcga gaccctgcag
     1021 gctcgccggg atggcctttg tcggcttgat catgacgagg tagtagagcg cagcctcgtc
     1081 accaccggcg ccctgctctc cgctgctgcc ctgtccctgc ccctgcccct gatcacccga
     1141 gccgccgctc ccgccggagt cgctgaccgg tgtcgcgccg acgctggaga ccacgcctgg
     1201 gtactgcttg ccggtgtcgt ccgagaagat ctgcaccttc tggccgacct tgaccctggc
     1261 gcgctggtag ggctgcagct tggccttgac ctgcatcgcc ccggaggaca ggacgagcat
     1321 gtcggtggga acggcgcggc cgagtggggc gttcatgctc acgacccgcg cggggagcgt
     1381 gcgcatgaag atgacctcgg acgcggggac cataggcccg gccttggccc gggcagcggt
     1441 cagtgcagtg agcgcacggt cacgatcctg ccgtgcgtag cgcagctgaa ggctgcgcga
     1501 ggtgctgccg gctccggtgc cgctcgcggt cccggtggcg cccccggttg tcgtccccac
     1561 t
//
LOCUS       DATAIX010000635         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_198911, whole genome shotgun sequence.
ACCESSION   DATAIX010000635 DATAIX010000000
VERSION     DATAIX010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198911"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VHM65_09385"
     CDS             <1..767
                     /locus_tag="VHM65_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX2705958.1"
                     /translation="RNIVTEDTLIPAPGSLDLPQTSVPLPNYDRTACGPGAKTTLLHA
                     FEISCNTAFGYLGLQIGGDGLREQAAKFGFGDTLRIPMRVTPSVVPADLNPPQTAQSA
                     IGQFDVRVTPLQVAMVSAAIANDGVVMRPYLVAQVLGANLESIDTTRPEQLSQAVTPE
                     VAAILKRMLVSVVDNGTGRTARIDGVSVGGKTGTAQQGNDKPPHAWFTAFAPAEDPQV
                     AVAVVVEDGGNAGNEAAGGRLAGPIAKAVIEAVISR"
     gene            764..>1694
                     /locus_tag="VHM65_09390"
     CDS             764..>1694
                     /locus_tag="VHM65_09390"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX2705959.1"
                     /translation="MSPTVGDVLGGRYTLLSRIAGGGMGEVWQAEDSVLRRLVAVKVL
                     RPNAADEPRFAERFRNEARHTAMLSHANIATVYDYGEDGGTAYLVMELVPGTPLSQVI
                     AERAPISPEETRRIMGQAANALAAAHAAGVVHRDVKPANIILTPEGTVKLTDFGIARA
                     LDGSSLTRTGEVIGTPNYLSPEQALGRGATAASDLYALGIVAHEMLTGARPFDSGSPV
                     ATALAQVNTEPPPLPPSVPAGLAAAVMQCLRKSPQDRPASAAALADALGMGPGMASGG
                     LGETRYPDAGSPTGAAAPTIALGAPVPPTVAEPV"
BASE COUNT          249 a          664 c          560 g          221 t
ORIGIN      
        1 cccgcaacat cgtcaccgag gacaccctga tcccggcccc cgggtcgttg gaccttcccc
       61 agacctcggt gcccctgccc aactacgacc gcaccgcatg cggccccggc gcgaagacga
      121 ccctgctgca cgccttcgag atctcgtgca acaccgcgtt cggctacctc gggctgcaga
      181 tcggcggcga cgggctgcgt gaacaggcgg cgaagttcgg cttcggtgac accctgcgga
      241 tcccgatgcg ggtcaccccg agcgtcgtgc ccgccgacct caacccgccc cagacggccc
      301 agtccgcgat cggccagttc gacgtgcggg tcacccccct gcaggtcgcc atggtcagcg
      361 ctgccatcgc caacgacggg gtcgtcatgc gcccctatct cgtcgcccag gtgctcggcg
      421 ccaacctcga gtcgatcgac acgacccgcc cggagcagct ctcgcaggca gtgacccccg
      481 aggtcgccgc gatcctcaag cgcatgctcg tctcggtcgt cgacaacggc accgggcgca
      541 cggcccggat cgacggcgtg tcggtcggcg gcaagaccgg gaccgcccag caaggcaatg
      601 acaagccccc ccacgcgtgg ttcacggcct tcgcgcccgc ggaggacccc caggtcgccg
      661 tcgcggtcgt cgtcgaggac ggcggcaacg ccggcaacga ggccgccggt ggccgcctcg
      721 ccggccccat cgcaaaggca gtgattgagg cggtgatctc acgatgagcc cgacagtcgg
      781 tgacgtcctc ggcgggcgct acaccctgct cagccggatc gcgggcggcg ggatgggcga
      841 ggtgtggcag gccgaggact cggttctgcg gcgcctcgtc gccgtcaagg tcctgcgccc
      901 caacgcggcg gacgagcccc ggttcgccga gcgcttccgg aacgaggccc ggcataccgc
      961 catgttgtcc cacgcgaaca tcgcgaccgt ctacgactac ggcgaggacg gcggcaccgc
     1021 ctacctcgtc atggagctcg tccccggcac cccgctgtcc caggtgatcg ccgagcgggc
     1081 ccccatcagc cccgaggaga cccggcggat tatggggcag gcggccaacg cgctcgccgc
     1141 ggcccacgcc gccggggtgg ttcaccgcga cgtcaagccg gccaacatca tcctgacgcc
     1201 cgaaggcacg gtgaagctca ccgacttcgg catcgcccgt gccctcgacg gctcgtcgct
     1261 gacccgcact ggtgaggtca tcggcacgcc gaactacctg tcgcccgagc aggccctcgg
     1321 gcgcggcgcg acggccgcga gcgacctcta tgccctcggc atcgtggccc acgagatgct
     1381 cacgggggcc aggcccttcg actcggggtc acccgtcgcc acggccctgg cgcaggtcaa
     1441 caccgagccg ccgccccttc ccccgagcgt cccggccggg ctggccgcgg ctgtcatgca
     1501 atgcctgcgc aagtccccgc aggaccggcc cgcgagtgcg gcggcgctcg ctgacgctct
     1561 gggcatgggc cccggcatgg ccagtggtgg cctcggtgag acccgatacc ccgatgcggg
     1621 gtcaccgact ggagctgccg cgccgacgat cgccctaggg gcgccggtcc cgcccaccgt
     1681 cgccgaaccg gtca
//
LOCUS       DATAIX010000636         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_199189, whole genome shotgun sequence.
ACCESSION   DATAIX010000636 DATAIX010000000
VERSION     DATAIX010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199189"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1222
                     /locus_tag="VHM65_09395"
     CDS             191..1222
                     /locus_tag="VHM65_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705960.1"
                     /translation="MVSSASRAESLAQKLLDAQVGYHLDRLTGDQLETTVSRLAEDLL
                     AAAGQHQIVDLVDRAAIKAIVVRALRSVPGSSAVSGIIELATTVVYDGPAESYPLGEP
                     VEREQIEVLLDELLALNPLLERALERLTDSPLVATMASRFMGRIVGEVLQANKAVADK
                     VPGLGSLMSFGTSAASKLKGAADKQLEALIGDTAGKGGTFAIRRLNRIVIETLRDPTT
                     REAVLQVWDLLSQETVAGLSPYATREEISGVVDAVHNLAVTTVAHEHVAHLGEVIVES
                     FFEWFGGYTPTELLDELDLSREDLVDDLMRIAPWAVEALRETGDLERMLRAQLEPFYT
                     STEVTVLLG"
     gene            complement(1303..>1555)
                     /locus_tag="VHM65_09400"
     CDS             complement(1303..>1555)
                     /locus_tag="VHM65_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705961.1"
                     /translation="GQGLGLSTVASHGRRWESRRGFTGLRVPDGGDRAGAALARMEGE
                     VGLRTLFERYPGLRIGQEAVRRNTRILRGCEQLLVTLSP"
BASE COUNT          247 a          537 c          523 g          248 t
ORIGIN      
        1 ctgcggcgca gcgccggacg accgcccggg atctggatgg tcggacttcc cgtgctggcg
       61 tccttcggca ctgaagggtc cggcacgatg gtcatttcgc cctcacgtag ccgatgagcg
      121 gcagcagagg tcacgcgtga gtggctagga gcgctacggt cctccgcgcg ccatcccgta
      181 ccgtttcccc atggtcagta gcgcttcgcg agcagagtcc cttgcccaga agctgctcga
      241 cgcgcaggtt ggctaccacc ttgaccggct gaccggagat cagctcgaga ccacggtgtc
      301 ccgtctcgcc gaggatctgc tggcggcggc gggccagcat cagatcgtcg acctcgtcga
      361 ccgggcggcg atcaaggcca tcgtggtgcg ggctctcaga agcgtgccgg gaagctccgc
      421 cgtcagcggc atcatcgagc tcgcgaccac ggttgtgtac gacggacccg ccgagtccta
      481 cccgctcggg gagcccgttg agcgcgagca gattgaggtg ctcctcgacg agctgctcgc
      541 cttgaacccc ctgctcgagc gggccttgga gcggctgacc gacagtccgc tggtcgccac
      601 gatggcgtcg cggttcatgg gccgcatcgt cggtgaggtc ctgcaggcca acaaggcggt
      661 cgcggacaag gtgcccgggt tgggctcgct gatgtcgttc gggacgagcg ccgcgtcgaa
      721 gttgaagggc gcggcggaca agcagctcga ggcgctgatc ggggacaccg ccggcaaggg
      781 tggcacgttc gccatacgcc gcctgaaccg gattgtcatc gagacgcttc gggaccccac
      841 gacccgcgag gctgtcctgc aggtctggga cctgctctca caggaaacgg tagccggtct
      901 gagcccctac gccacgcgcg aggagatctc cggtgtggtc gacgccgtgc acaacctggc
      961 ggtcaccacc gtcgcccacg agcacgtcgc gcacctcggc gaggtgatcg tcgagagctt
     1021 cttcgagtgg ttcggtggct acaccccgac cgagctgctc gacgagctcg acctcagccg
     1081 tgaagacctc gtcgacgacc tgatgcggat cgcgccgtgg gccgtcgagg cgctgcggga
     1141 gacgggcgac ctggagcgga tgctgcgggc ccagcttgag ccgttctaca cctcgaccga
     1201 ggtcaccgtc ctgctgggct gactggtcag gtcgtcggtt cgggctcgat ctccgatccc
     1261 aggacacatg agcaccggca ctcgccccag ggcctcagaa cttcaggggc tcagcgtgac
     1321 aagaagctgc tcgcagccgc gcaagatgcg cgtgttccga cgcacggctt cttgcccgat
     1381 ccgtaggccg gggtaccgct cgaacagcgt ccgcagtccg acctcccctt ccatgcgggc
     1441 cagcgccgcg ccagcccgat cacctccgtc gggaacccgg agcccggtga acccccgacg
     1501 ggactcccac cggcgaccgt gactggcgac cgtgctcagc ccaagaccct gaccc
//
LOCUS       DATAIX010000637         3172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_199397, whole genome shotgun sequence.
ACCESSION   DATAIX010000637 DATAIX010000000
VERSION     DATAIX010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199397"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VHM65_09405"
     CDS             complement(<1..330)
                     /locus_tag="VHM65_09405"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2705962.1"
                     /translation="MDNVTRRSVDQAETIAANRSWWDHEADGYYAEHGGFLGDSGFVW
                     GPEGLREADLHLLGDVTGSRVLEIGAGAAQCGRYLATQGATVVATDLSMGMLRQGARL
                     NAVSAPVE"
     gene            368..1291
                     /locus_tag="VHM65_09410"
     CDS             368..1291
                     /locus_tag="VHM65_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705963.1"
                     /translation="MSEILPESSQRWCTETLGSPAVELLSSRRLMSDSRGVRLADGRE
                     VMVKRRWDDHGRAAVCVQAQRALADAGMPVARPLTGVHLVDGDAVHAETWVPGGQMMR
                     RDDTGAAAIMARLLARVQDALDALGVAPPLPNADWVRWYRDDDALWAPNAYFDEHCAE
                     VELPGELTRIARRCRARLLDHDLPFTVGHADWEVQNLRFDGEQIHVIHDWDSLAWLPE
                     AALAGTAAGGFVSQERPTLAPLRSSEVFLEEYQHARGRSFTGQELEIAWAASLWIAVH
                     NARAELLVQREASAWPQVQLQAGERLSRAGA"
     gene            1487..2965
                     /gene="rpsA"
                     /locus_tag="VHM65_09415"
     CDS             1487..2965
                     /gene="rpsA"
                     /locus_tag="VHM65_09415"
                     /inference="COORDINATES: protein motif:HMM:NF005911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX2705964.1"
                     /translation="MTATTVTKTAPQVAINDIGTEEDLLAAIDATIKDFNDGDIVEGV
                     IVKVDRDEVLLDIGYKTEGVIPSRELSIKHDVDPSDVVKVGDEVEALVLQKEDKEGRL
                     ILSKKRAQYERAWGTIEKVKEEDGAVTGTVIEVVKGGLILDIGLRGFLPASLVEMRRV
                     RDLQPYVGKQIEAKIIELDKNRNNVVLSRRAWLEQTQSEVRTTFLKELQKGQVRTGVV
                     SSIVNFGAFVDLGGVDGLVHVSELSWKHIDHPSEVVEVGTEVTVEVLDVDMDRERVSL
                     SLKATQEDPWQHFARTHAIGQVVPGKVTKLVPFGSFVRVDDGIEGLVHISELAERHVE
                     LPEQVVQVGADIFVKVIDIDLERRRISLSLKQANDSGSQVAEFDPTLYGMAAEYDEGG
                     NYKYPEGFDPETNEWLPGFETQREKWEKQYAEAHSRWEAHKAQVQAAASADSEAAVSS
                     GEGISSYSSASSTADSGPAAPAEGTLASDEALAALREKLTGN"
BASE COUNT          497 a         1104 c         1107 g          464 t
ORIGIN      
        1 ctccacgggt gccgacacgg cgttgagccg ggcgccctgg cgcagcatgc ccatcgacag
       61 gtcggtggcc acgacggtgg ctccctgggt ggcgaggtag cggccgcact gggcagcacc
      121 cgcgccgatc tccagcaccc ggctcccggt gacgtcaccg agcaggtgca ggtcggcctc
      181 gcgcagaccc tcgggtcccc acacgaagcc ggagtcgccg aggaagccgc cgtgctcggc
      241 gtagtagccg tcggcctcgt ggtcccacca tgaccggttg gccgcaatcg tctcggcctg
      301 gtcgaccgag cgccgcgtca cgttgtccat gtggccagtc tgccccgcgg gcgggcacga
      361 tgctcgggtg agcgagatcc tgcccgagtc gagccagcgc tggtgcaccg agacgctggg
      421 ctcgcccgcg gtcgagctgc tgtcctcgcg ccggctcatg tccgactcgc ggggcgtccg
      481 gctcgccgac gggcgcgagg tcatggtcaa gcgccggtgg gacgaccatg gccgggccgc
      541 ggtgtgcgtg caggcccagc gtgctctcgc cgatgccggt atgccggtcg cccggcccct
      601 gacgggggtg cacctcgtcg acggggacgc cgttcatgcc gagacgtggg tccccggcgg
      661 gcagatgatg cgtcgcgacg acaccggggc ggccgcgatc atggcgcggc tcctggcccg
      721 ggtgcaggac gccctcgacg cgctcggggt cgcgccgccg ctgcccaatg ccgactgggt
      781 caggtggtac cgcgacgacg acgcgctctg ggctccgaac gcgtacttcg acgagcactg
      841 cgccgaggtc gagctgcccg gggagctgac ccggatcgcg cggcggtgcc gcgcccggct
      901 gctcgaccat gacctcccgt tcaccgtcgg gcacgccgac tgggaggtcc agaacctgcg
      961 gttcgacggt gagcagatcc acgtcatcca cgactgggac agcctggcct ggctgcccga
     1021 ggccgccctg gcggggacgg ccgcgggggg cttcgtgagc caggagcgcc cgacgctggc
     1081 ccccctgcgc tcatcggagg tcttcctcga ggagtaccag cacgcccggg ggcggtcatt
     1141 cactgggcaa gagctcgaga tcgcgtgggc ggccagcctg tggatcgccg tgcacaacgc
     1201 ccgcgccgag ctgctcgtcc agcgtgaggc gagcgcctgg ccacaggtgc agctccaggc
     1261 cggagagcgc ctgagccgcg ctggggcgtg agccggccgg cataccccct ggcgcttgac
     1321 gaccgcgctt tgacccatcg cggagcccgc aggtaggctg gctcgtcgca cacgtgtgcg
     1381 ctgattcacc ctgtccgcat ggccctggcc gcgatgaccc gcgacctggc gtcccgagtt
     1441 aacactgtcc acaattaaca ctgtccacaa tcggagcccc tcctacatga ctgccactac
     1501 ggtcaccaag accgcccctc aggtcgcgat caacgacatc ggcactgagg aagacctgct
     1561 ggccgcaatc gatgcgacca tcaaagactt caacgacggc gacatcgtcg aaggggtcat
     1621 cgtcaaggtc gaccgggacg aggtcctgct cgacatcggc tacaagaccg agggtgtcat
     1681 cccctcgcgt gagctgtcca tcaagcacga cgtcgacccc tccgatgtcg tcaaggtcgg
     1741 cgacgaggtc gaggccctgg tcctgcagaa ggaggacaag gaaggccggc tgatcctgtc
     1801 caagaagcgc gcgcagtacg agcgcgcgtg gggcacgatc gagaaggtca aggaggagga
     1861 cggcgccgtc accggcacgg tcatcgaggt cgtcaagggt ggtctcatcc tcgacatcgg
     1921 cctgcgtggc ttcctgcccg cgtccctcgt cgagatgcgc cgcgtgcggg acctgcagcc
     1981 gtatgtcggc aagcagatcg aggcgaagat catcgagctg gacaagaacc gcaacaacgt
     2041 ggtcctgtcc cgccgggcct ggctggagca gacgcagtcc gaggtccgga ccaccttcct
     2101 caaggagctc cagaagggcc aggtccgcac gggtgtcgtg agcagcatcg tcaacttcgg
     2161 tgcgttcgtc gacctcggcg gggtcgacgg tctcgtccac gtctccgagc tgtcgtggaa
     2221 gcacatcgac cacccgagcg aggtcgtcga ggtcggcacc gaggtcaccg tcgaggtgct
     2281 cgacgtcgac atggaccgcg agcgcgtctc gctgtcgctc aaggcgaccc aggaggaccc
     2341 gtggcagcac ttcgcccgga cccacgcgat cggtcaggtc gtgccgggca aggtcaccaa
     2401 gctcgtgccg ttcggctcgt tcgtgcgggt cgatgacggc atcgagggtc tggtccacat
     2461 ctccgagctg gccgagcgcc acgtcgagct gcccgagcag gtcgtgcagg tgggcgcgga
     2521 tatcttcgtc aaggtcatcg acatcgacct cgagcgccgg cggatctcgc tgtcgctcaa
     2581 gcaggccaac gactccggct cgcaggtcgc cgagttcgac ccgacgctct acggcatggc
     2641 cgcggagtac gacgaggggg gtaactacaa gtaccccgag ggcttcgacc cggagacgaa
     2701 cgagtggctg ccgggcttcg agacccagcg cgagaagtgg gagaagcagt acgccgaggc
     2761 ccactctcgc tgggaggccc acaaggccca ggtccaggcg gcggcgtcgg ccgactccga
     2821 ggcagccgtg tcctcgggtg agggcatctc gtcctactcg tccgcgtcca gcacggctga
     2881 ctccggtccg gccgcgccgg ccgagggcac cctcgcctcg gacgaggcac tcgccgcact
     2941 gcgcgagaag ctcaccggca actgatccac ggtatgccgt gacaggcccc ggttcccctc
     3001 gcagggaccg gggccgatca cgtggtcccc cccgcacggt gtcagccggc gaggcgatcc
     3061 agacccgtgc ggagggcgcg ctctgcggcc tgctccaacg cgtcgtggag cgggctgtcg
     3121 cccaggtcgt ccgcggcctt ctggaactcg accccgacga tggtccggca cg
//
LOCUS       DATAIX010000638         1519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_136893, whole genome shotgun sequence.
ACCESSION   DATAIX010000638 DATAIX010000000
VERSION     DATAIX010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136893"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VHM65_09420"
     CDS             <1..414
                     /locus_tag="VHM65_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705965.1"
                     /translation="IRRSAFEVVGGWPGHFWYGHEGIDLAFRLVDAGWRLHYCPQVVV
                     SHPATSATRHAVFYRMNARNRVWVARRNLPWPLVPIYLGTWAVLTVARVRDRERLRVW
                     FRGFSEGLTSDAGLRRPMSWRTVAHLTRLGRPPVV"
     gene            447..1463
                     /locus_tag="VHM65_09425"
     CDS             447..1463
                     /locus_tag="VHM65_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705966.1"
                     /translation="MRISLSGVRSLTHLVYAASYVRAVLAESSGPVTVLDLGVGTFLG
                     RATVTESDVARVLPTHERLTVTGPGAAGRTATGPRATSYRHDYLSVGAPGIKPYLRLV
                     ARDPRHRPRVVVLDEGLGSYGDWHTRRDAWRRQGGREPWPTLRSWAVSSANQILTDVR
                     WSLFEQAVTGWVVAPRVGAEFQRHTYGQPGRPGRAVFLTQPWVELGLVSASRYVEHLT
                     AVAAVCAEAGLDLQVRAHPAEDRTKYAGIALDPSTGLAELDRSVVSADVVLGGNSTAL
                     LNLAAVHERPALRVGLPELAHLDAQLGPRQRSLLDTYLPPVVDHRADLMPGLLRAFGG
                     AQPE"
BASE COUNT          178 a          551 c          540 g          250 t
ORIGIN      
        1 atccggcgca gcgcgttcga ggtggtcggg gggtggcccg gtcacttctg gtatggccac
       61 gaagggatcg acctcgcctt ccgccttgtc gacgcgggct ggcggctgca ctactgcccc
      121 caggtcgtcg tcagccaccc ggcgaccagc gcgacccgcc acgcggtgtt ctaccgcatg
      181 aacgcgcgca accgggtctg ggtcgcccgg cgcaacctgc cctggcccct cgtgccgatc
      241 tacctcggca cgtgggcggt cctcaccgtg gcccgcgtcc gggatcggga gcggctgcgg
      301 gtctggttcc gcggcttctc cgaaggcctc acctccgatg ccgggcttcg ccggccgatg
      361 tcgtggcgca ccgtcgccca tctgacccgc ctcggccgcc cgccggtcgt atgacgccca
      421 cccccgggga cccgaccgcc cgaccggtgc gcatcagcct gtccggggtc cggagcctga
      481 cccatctggt ctatgcggcg tcctatgtgc gggccgtcct ggccgagtcc tccgggccgg
      541 tgacggtgct cgacctcggt gtcggcacgt tcctcggtcg agccacggtg accgaatcgg
      601 atgtggcccg ggtgctgccc acccacgagc ggctcaccgt gacaggcccc ggcgccgcgg
      661 gccggaccgc gacggggccg cgtgcgacgt cataccggca tgactatctg tccgtgggtg
      721 cccctggtat caagccctat ctgcgcctcg tggctcgcga cccgcgccac cggccacgcg
      781 tggtagtcct cgacgagggg ctcggctcgt acggcgactg gcatacccgt cgcgacgcat
      841 ggcgccgcca gggtggtcgc gaaccgtggc cgaccctgcg gtcgtgggcg gtgtcgagcg
      901 ccaaccagat cctgaccgac gtccgctggt cgctcttcga gcaggctgtg acggggtggg
      961 tcgtggcgcc acgggtcggt gcggagttcc agcggcacac gtacgggcag cccggacgtc
     1021 ccgggcgggc ggtcttcctg acccagccgt gggtcgagct cgggctcgtc agcgcgtccc
     1081 ggtatgtcga gcacctcacc gccgtggcgg cggtatgcgc cgaggccggt ctcgatctcc
     1141 aggtcagagc gcaccccgcc gaggaccgga ccaagtatgc cgggatcgca ctcgacccga
     1201 gcacgggtct ggccgagctg gaccggtcgg tggtctcggc cgacgtggtg ctcggcggca
     1261 acagcacggc cctgctcaac ctcgccgcgg tccacgagcg tccggcgttg cgggtgggcc
     1321 tgccggagct ggcgcacctg gacgcccagc tcgggccgcg ccagcgcagc ctgcttgaca
     1381 cctatctgcc tccggtcgtc gaccatcgcg ctgacctcat gcccggtctg ttgcgtgcgt
     1441 ttgggggtgc gcaaccagag tgaccgtgtg acgattgcgg tgattgacgg atgagcccgg
     1501 tttctgtggg ccgccgttg
//
LOCUS       DATAIX010000639         1637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_289834, whole genome shotgun sequence.
ACCESSION   DATAIX010000639 DATAIX010000000
VERSION     DATAIX010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289834"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VHM65_09430"
     CDS             <1..424
                     /locus_tag="VHM65_09430"
                     /inference="COORDINATES: protein motif:HMM:NF024682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705967.1"
                     /translation="LIGRFVHAVELATRERHGRGRLTRYGAGLAIPGEVRAEIAVLKG
                     TAAHFVMLVDERLAVMALQRDAVADLVAAYRADPLGRLDPDLRADFAAAADDVARLRV
                     VVDQVASLTDARAWNLHAQWSAGQWSTTAAPTTSAPTT"
     gene            451..>1637
                     /gene="dnaG"
                     /locus_tag="VHM65_09435"
     CDS             451..>1637
                     /gene="dnaG"
                     /locus_tag="VHM65_09435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX2705968.1"
                     /translation="MAGLIKAEDIAAIKERSSIEDVVREHVTLRSAGPGSLKGLCPFH
                     DEKTPSFTVRPAVGVYHCFGCGEGGDVIAFVEKVEHLTFTEAVERLAGRLGMELRYDE
                     GSGRAREDTVGRRTRLVEAHRAAEEFYTERLLGSAEARPGRDFMRERGFDSGVARRFG
                     VGFAPRGGEELAAYLRGKGFGDDELVASGLAGRGRYGLYDKFRGRLVWPIRDITGATV
                     GFGARRIFEDDRMEAKYLNSAETPIYKKSSVLYGLDFAKKAIARDRKAVVVEGYTDVM
                     ACHLAGVESAVATCGTAFGVEHIKVLRRLMRDEQDQTPARVIFTFDGDAAGQKAAMRA
                     FGEDQRWASQSFVAVEPSGMDPCELRQSGGDPAVVALVEDAVPMFEFAVRTTLRRFDL
                     TTA"
BASE COUNT          219 a          565 c          609 g          244 t
ORIGIN      
        1 gctcatcggc cgcttcgtgc acgccgtcga gctcgccacc cgcgaacggc acggccgcgg
       61 acgcctgacc cggtatggcg caggcctcgc catcccgggc gaggtgaggg ccgagatcgc
      121 cgtgctcaag gggacggccg cccacttcgt catgctcgtc gacgagcgcc tcgcggtgat
      181 ggcgctccag cgcgacgcgg tggccgacct cgtcgcggcc taccgggctg acccgctggg
      241 ccgcctcgac ccggatctgc gcgccgactt cgccgccgcc gccgacgacg tcgcgcggct
      301 gcgcgtcgtc gtcgaccagg tggcctcgct caccgatgcg cgggcgtgga acctgcacgc
      361 acagtggtcg gctggacaat ggtcgacgac ggccgcgccg acgacctccg caccgacgac
      421 ctagactccc cgtgtcggaa gggagcgcgc gtggccgggc tgatcaaggc cgaggacatc
      481 gctgccatca aggagcggtc ctcgatcgag gacgtcgtgc gcgagcatgt cacccttcgc
      541 tcggccggcc ctggctcgct caagggcctg tgccccttcc acgacgagaa gacgccgtcc
      601 ttcaccgtgc gccccgccgt cggggtctac cactgcttcg ggtgcggcga gggtggcgat
      661 gtcatcgcct tcgtcgagaa ggtcgagcac ctcaccttca ccgaggcggt cgagcgcctc
      721 gccggacggc tcgggatgga gctgcggtat gacgaggggt ccggtcgcgc gcgcgaggac
      781 accgtcggtc ggcgcacccg gctcgtcgag gcccaccggg cggccgagga gttctacacc
      841 gagcggctgc tcgggtcggc cgaggcccgc cccggtcggg acttcatgcg tgagcggggc
      901 ttcgactcgg gcgtggcccg ccggttcggg gtggggttcg cgccgcgcgg tggcgaggag
      961 ctcgcggcat acctgagggg taagggtttc ggcgacgacg agctcgtcgc gagcgggctg
     1021 gcggggcgtg ggcgctacgg gctctacgac aagttccgcg ggcggctcgt gtggccgatc
     1081 cgggacatca ccggggcgac ggtggggttc ggcgcccgcc gcatcttcga ggacgaccgg
     1141 atggaggcca agtacctcaa ctcggccgag acgccgatct acaagaagtc ctcggtcctc
     1201 tacggcctcg acttcgccaa gaaggccatc gcgcgtgacc gtaaggcggt ggtcgtcgag
     1261 ggttacaccg atgtcatggc ctgccatctt gccggggtcg agtcagcggt ggcgacctgt
     1321 gggacggcct tcggcgtcga gcacatcaag gtcctgcgcc ggctcatgcg cgacgagcag
     1381 gaccagacgc cggcccgggt catcttcacc ttcgacggtg acgccgccgg ccagaaggcg
     1441 gcgatgcggg cgttcggcga ggaccagcgc tgggcgtcgc agtcgttcgt ggccgtcgag
     1501 ccgtccggga tggacccgtg cgagctgcgc cagtccgggg gcgacccggc cgtcgtggcg
     1561 ctcgtcgagg acgccgtgcc gatgttcgag tttgccgtgc gcacgacgct gcgccggttc
     1621 gacctgacga ccgccga
//
LOCUS       DATAIX010000640         2730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_229676, whole genome shotgun sequence.
ACCESSION   DATAIX010000640 DATAIX010000000
VERSION     DATAIX010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229676"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VHM65_09440"
     CDS             complement(<1..977)
                     /locus_tag="VHM65_09440"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX2705969.1"
                     /translation="MARTPAPGAQVSGTVLRGDVTARDFADALDALGGLNPGRAVILV
                     ALAPPAPVSAAVVDLVVDELLARGADSVTVGAGRGVSAHALAAGLTGHTARGRTYDVI
                     DVHDSLGTADLPVGSVLHGRPVSLAWSGATTRVVLARSVTDLAEGYGGALATLATIAA
                     EVPGADPAEVAADLADHLAPHLCIIDATISSDGPDGRHQLRPADTGALVVGLDPLVVD
                     ATLAGLHGIDRAVSRPVAELLRRRGPPTGEVSGDLTPFDAFERPHPLIRDAWRRAEQA
                     PGIGRVLAAARAGRGRGAELGQGAAGAVAHHDHRRGVEPVGRAVGPHPL"
     gene            1204..2724
                     /locus_tag="VHM65_09445"
     CDS             1204..2724
                     /locus_tag="VHM65_09445"
                     /EC_number="2.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF008984.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA carboxytransferase subunit 5S"
                     /protein_id="HEX2705970.1"
                     /translation="MGHTGDVDIQREIGVTELALRDAHQSLMATRMAIEDMVGACEDI
                     DQAGYWSVECWGGATFDACIRFLNEDPWERLRTFRTLLPNSRLQMLLRGQNLLGYRHY
                     GDDVVDHFVEKAAENGIDVFRVFDALNDTRNVERAITAVKKAGKHAQGTICYTTSPLH
                     TVEGYVRQARELHDLGVDSLALKDMAALLKPQPAFDIVSAIKAEIPGMQVNLHCHAIT
                     GVTLVSLMKAIEAGADVVDTAISSLSLGPGHNPTESLQEMLEGTGYIADLDKVRLGKI
                     KDHFAEIRPRYAAFMSSFNVETDIFESQIPGGMISNMESQLKQQGAGDRLREVLEEVP
                     RVRKDAGYPPLVTPSSQIVGTQAVFNVMMGRYKVLTAEFSDLMLGYYGYTMGEKNPEV
                     IEAARTQTGKEIITSRPADLISAEWSVLRTEAEAIEGFDGSDEDVLTYAMFPKVAPDF
                     FLSRPDGPKNVGKGPAELEAERVAAAADPKKGPVRGPIKYEITINGKSHSVAVKPA"
BASE COUNT          462 a          956 c          951 g          361 t
ORIGIN      
        1 tcgagtgggt gagggcccac ggcgcggccc acgggctcga cccctcgacg gtggtcgtgg
       61 tgggccacag cgccggcggc accctggccg agctcagcgc cgcggccccg gccggcccgg
      121 gccgcggcga gcacccggcc gatgcccggc gcctgctcgg cgcggcgcca cgcgtcccgg
      181 atcagggggt ggggccgctc gaacgcgtcg aagggggtca ggtccccgct cacctcgccg
      241 gtgggggggc cacggcgccg caggagctcg gcgaccggtc gggacacggc tcggtcgatg
      301 ccgtgcagcc cggccagcgt ggcgtcgacg acgagcgggt cgagcccgac gacgagggcg
      361 ccggtgtccg cgggccggag ctgatgacgc ccgtcggggc cgtccgagga gatcgtggcg
      421 tcgatgatgc acaggtgcgg tgcgaggtgg tcggccaggt cggccgcgac ctcggcggga
      481 tcggctccag ggacctccgc ggcgatcgtc gcgagggtgg ccagggcgcc gccatacccc
      541 tcggcgaggt cggtgaccga tctcgcgagg acgacccgcg tcgtcgcacc gctccaggcc
      601 aggctcaccg gccggccgtg cagcacggac cccacgggga ggtcggccgt cccgagcgag
      661 tcgtggacgt cgatgacgtc atacgtgcgg ccacgcgcgg tatgtccggt gaggcccgcc
      721 gccagggcat gggcggacac gccgcgccca gcccccacgg tcacgctgtc ggcccctcgg
      781 gccaggagct cgtcgacgac caggtcgaca accgccgccg agacgggggc gggcggggcg
      841 agcgcgacga gtatcaccgc ccggcccggg ttcagcccgc cgagggcgtc gagggcgtcg
      901 gcgaagtccc gggccgtgac atccccacga aggacggtcc cggacacctg agcgccgggc
      961 gccggggtcc gggccatccc accatgaaag cagacggtcg gggtccgccc cgcacgcgcc
     1021 gctccaccac gggtcgcggc cacccgaaca ggcccaacct catcggcagt ccggcatacc
     1081 gggacacgat ggacatctgg cgagcgttcg gcctgttgaa ccgtccgctg acaccggcac
     1141 ggccggtctg gtcggtgcca tgacggcagt cgggacccgg ccaggggatg cgtcagcgcc
     1201 gaaatgggcc atactggcga cgtggatatc cagcgagaga tcggcgtgac cgagctagcc
     1261 ctccgggacg cgcaccagag tctgatggcc acccgcatgg cgatcgaaga catggtgggt
     1321 gcctgcgagg acatcgacca agcggggtac tggagcgtcg agtgctgggg gggtgccacc
     1381 ttcgacgcct gcatccggtt cctcaacgag gatccgtggg aacgactgcg aacgttccgc
     1441 accctgcttc ccaacagccg cctgcagatg ctgctgcgtg gccagaacct gctgggctac
     1501 cgccactacg gcgacgacgt cgtcgaccac ttcgtcgaga aggccgccga gaacggcata
     1561 gatgtcttcc gggtgttcga cgccctcaac gacacccgca acgtcgagcg cgccatcacc
     1621 gccgtcaaga aggccggcaa gcacgcgcag ggcacgatct gctacacgac gagccccctg
     1681 cacaccgtcg agggctacgt gcgccaggcc cgcgagctgc acgatctcgg cgtcgactcc
     1741 ctggccctca aggacatggc ggccctgctc aagccgcagc ctgcctttga catcgtgtcg
     1801 gcgatcaagg ccgagatccc cggcatgcag gtcaatctgc actgccacgc catcaccggg
     1861 gtcacgctgg tgtcgctgat gaaggcgatc gaggccggcg cggacgtcgt cgacaccgcc
     1921 atcagctcgt tgtcgctcgg tcccggtcac aaccccaccg agtcactgca agagatgctc
     1981 gaaggcacgg gctacatcgc cgacctcgac aaggtccggc tggggaagat caaggaccac
     2041 ttcgccgaga tcaggccccg ctacgcggcg ttcatgtcct cgttcaacgt ggagaccgac
     2101 atcttcgaaa gccagatccc tggcggcatg atctccaaca tggagtccca gctcaagcag
     2161 cagggcgcgg gtgacaggct gcgcgaggtg ctcgaagagg tcccgcgggt tcgcaaggac
     2221 gccggctatc ctccgctggt cacgccatcg agccagatcg tgggaaccca ggcggtcttc
     2281 aacgtgatga tgggccgcta caaggtcctg accgccgagt tctccgacct gatgctcggc
     2341 tactacggct acaccatggg agagaagaac cccgaggtca tcgaggccgc caggacccag
     2401 accggtaagg agatcatcac ctcccgtcca gccgacctca tctccgccga gtggtcggtc
     2461 ctgcgcaccg aggcggaggc catcgagggt ttcgacgggt ccgacgagga cgtgctgacc
     2521 tacgcgatgt tcccgaaggt ggcgccggac ttcttcctca gccgccccga tggtcccaag
     2581 aacgtcggga agggccctgc cgagctggag gcagagcgtg tcgccgccgc cgccgatccc
     2641 aagaagggac ccgtgcgcgg cccgatcaag tacgagatca ccatcaacgg caagagtcac
     2701 tccgtcgccg tgaaacccgc ctaggagagt
//
LOCUS       DATAIX010000641         4361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_256219, whole genome shotgun sequence.
ACCESSION   DATAIX010000641 DATAIX010000000
VERSION     DATAIX010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256219"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VHM65_09450"
     CDS             <1..467
                     /locus_tag="VHM65_09450"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX2705971.1"
                     /translation="ITPEQLALIAARAMVPKPKPAIMLSHLHAPTVSVREQAAIIVAR
                     LRRTGASSFRTLVADADTTLLIVARFLALLELFRESVIAFEQAEALGELTVRWTGTEQ
                     GDIEVSDEFDDGIEREAERAAEPEGDVKTQTLIETARGVITRGDIPKEPTDE"
     gene            460..1083
                     /gene="scpB"
                     /locus_tag="VHM65_09455"
     CDS             460..1083
                     /gene="scpB"
                     /locus_tag="VHM65_09455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX2705972.1"
                     /translation="MSEDVSSTQTREEPEDPAGFDAADVPGGVRSAIEAVLMVVDEPV
                     SEVALASALELPVADVLGYLDELEREYADAERGFAIRQVAGGWRIYSRPDYAPVVERF
                     LLDGQQARLTQASLETLAVVAYKQPVTRSRVSAIRGVNVDGVVRTLVSRGLIHEIDDA
                     GDGGAIRYGTTPYFLERMGLLALDELPALAPYLPDADALDELSEQGL"
     gene            1413..2129
                     /locus_tag="VHM65_09460"
     CDS             1413..2129
                     /locus_tag="VHM65_09460"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX2705973.1"
                     /translation="MRLQKLLAAAGVGSRRVCETLITAGRVEVDGQVVTELGVRIDPT
                     RRSVRVDGERVQLDTSRVYLAFNKPRGVVSTMSDDLGRTSIADFVGNRSERLFHVGRL
                     DADTEGLLLLTNDGELAHRLQHPAYGVLKTYLAQVPGPIPRDLGRRLREGVELDDGPV
                     RVDSFRVVDSMPGKALVEVALHEGRNHVVRRLLDAVGHPVQTLVRTDVGPVHLGDLRP
                     GRTRALTASEVGTLYGAAGL"
     gene            2166..3239
                     /locus_tag="VHM65_09465"
     CDS             2166..3239
                     /locus_tag="VHM65_09465"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF005111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEX2705974.1"
                     /translation="MTAIRVIGTGLIGTSAGLALSRAGYDVTLEDPSPTAQTLARDLG
                     AGTLDADAIPDLVVVAAPPDVTGSVVASALGRWPSAVVTDVASVKVAILRDVVSRRAD
                     LSRYVGSHPMAGRERSGTIAARGDLFQGRAWVVVPHEGSDPDAVALVARVATDTGAAV
                     RLMDPEGHDAAVAAVSHVPQVAASLVAARLRDLTEPALALSGQGLRDVTRIAASDERL
                     WTQILAGNAGAVREVLAALHADLGEVIAALATVGTAEGVGALEVLARTIGNGNLGYAL
                     IPGKHGAAPTTYAIVAVVVPDEPGMLGRLLVAVGEAGVNLEDLELEHGLGLPFGIAEI
                     AVLPGSAEHLARELTRRGWRLHS"
     gene            3236..3931
                     /gene="cmk"
                     /locus_tag="VHM65_09470"
     CDS             3236..3931
                     /gene="cmk"
                     /locus_tag="VHM65_09470"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX2705975.1"
                     /translation="MSTGLVVAIDGPSGSGKSSVSRAVASRLGVGYLDTGAMYRALTW
                     WCLERRLDLDDADAVAAAAAALPLVIGLDAQAPTVEVDGADVGAAIRETRISEAVSAV
                     ATNLAVRANLRGRQRRLIAEAVASRGGVVAEGRDITTVVAPDADVRVLLTASEAARLQ
                     RRARELLGSADAASLAAIHAQIVHRDQRDSTVSQFTEAADGVVLVDTSGLDLDGSVEA
                     VLQVVAAVSGRRG"
     gene            3993..>4361
                     /locus_tag="VHM65_09475"
     CDS             3993..>4361
                     /locus_tag="VHM65_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX2705976.1"
                     /translation="MLFGAGYRCRTLGTARSVLRAGGVAGVFPEGTRGRGDVAQVSQG
                     ATWLAPHSGAQIVPVEVQLGRVDEDHTVGGLGELRDGGVAPVPADDLLAGGGKAGGVG
                     CAVQLTGAPREPRGLARGEEH"
BASE COUNT          565 a         1590 c         1612 g          594 t
ORIGIN      
        1 gcattactcc tgagcagctc gccctcatcg ccgcccgggc catggtgccc aaacccaagc
       61 cggccatcat gctgagccac ctccacgccc cgacggtcag cgtccgcgaa caggccgcga
      121 tcatcgtggc ccgtctgcgg cgcaccggtg ccagctcgtt ccgaacgctc gtcgccgatg
      181 ccgacacgac gttgctcatc gtggcccggt tcctcgccct gctcgagctg ttccgcgagt
      241 cggtgatcgc cttcgagcag gccgaggcac tcggcgagct caccgtgcgc tggacgggaa
      301 ccgagcaggg tgacatcgag gtcagcgacg agttcgacga cggcatcgag cgcgaggccg
      361 agcgtgcggc cgagcccgag ggtgacgtga agacccagac cctgatcgaa acggcgcgcg
      421 gcgtgatcac ccgtggcgac atacccaagg agcccactga tgagtgagga cgtgtcctcg
      481 acccagaccc gcgaggagcc cgaggacccg gccggcttcg acgccgccga cgtgcccggg
      541 ggggtgcgct cggcgatcga ggccgtcctc atggtcgtcg acgagccggt gtccgaggtc
      601 gcgctggcct cggcgctgga gctcccggtg gccgatgtcc tgggctacct cgacgagctc
      661 gagcgcgagt atgccgacgc cgagcgcggg ttcgcgatcc gtcaggtcgc cggtggctgg
      721 cggatctaca gcagaccgga ctacgccccg gtcgtcgagc ggttcctgct cgacggccag
      781 caggcccggc tgacccaggc gtcgctggag accctggccg tcgtggcgta caaacagccc
      841 gtcacgcgat cgcgggtcag cgcgatccgg ggggtcaatg tcgatggggt ggtgcggact
      901 ctggtgtccc gcggactgat ccacgagatc gacgacgccg gcgacggcgg ggcgatccgc
      961 tacggcacga caccatactt cctcgaacgc atggggctgc ttgccctcga cgagctgccg
     1021 gccctcgcgc catacctgcc ggacgccgac gccctcgacg agctgtccga acaggggctg
     1081 tgatgacgcc cccgtcccgc cgagggccag cgggacaggg ccagccgggc agagggtcct
     1141 ccgccggggg gtctgcgggc aagggatctg caggcaaggg gtcatcgggc cgggcggctg
     1201 ctcggacggg ctcgggctcc ggtggctcgg tcggcaaggg atctgcgggc cgccagggcg
     1261 ctcgggccgg cggctccggc cgaggccctt cgcggcccgc ctcctggtcg gccaaggcgc
     1321 cgcgccacgc cggcacgacc aaggcgagcc agccgaccag gctcccgcgg tcgagccccg
     1381 tcgccccggt cgatgtccac gaccccgacg gcgtgcgact gcagaagctc ctcgccgcag
     1441 cgggggtcgg ctcacggcga gtctgcgaaa ccctcatcac cgcggggcgg gtcgaggtcg
     1501 acgggcaggt cgtgaccgag ctcggcgtcc ggatcgaccc gacccgccgc agcgtccggg
     1561 tcgacggcga gcgggtccag ctcgacacct cgcgggtcta cctcgcgttc aacaagccac
     1621 gcggtgtcgt ctcgacgatg agcgacgacc tcggccggac ctcgatcgcc gacttcgtcg
     1681 gcaaccgcag cgagcggctc ttccacgtcg gccgcctcga cgccgacacc gagggcctgc
     1741 tcctgctcac caacgacggc gagctggccc accggctcca gcacccggcc tacggcgtcc
     1801 tcaagaccta cctcgcccag gtgcccggac cgatcccgcg ggacctcggc cgacggctgc
     1861 gcgagggcgt cgagctcgac gacggacccg tgcgcgtcga ctcgttccgg gtcgtcgact
     1921 cgatgccggg caaggccctc gtcgaggtcg ccctgcacga ggggcgcaac cacgtcgtcc
     1981 ggcggctgct cgacgcggtc ggtcaccccg tgcagacgct ggtgcgcacc gatgtcgggc
     2041 cggtgcacct cggtgacctg cggccgggac ggacacgcgc cctcaccgcg agcgaggtcg
     2101 gcaccttgta cggcgcggct ggactctgac gacagcgccc cccggggctc gctacccttc
     2161 gcaccgtgac ggcgatccgg gtcatcggca ctggtctcat cgggaccagt gccggcctcg
     2221 cgctgtcccg ggccgggtac gacgtcaccc tcgaggaccc ttccccgaca gcgcagaccc
     2281 tcgcccggga cctcggcgca gggaccctgg acgcggacgc cataccggac ctcgtcgtcg
     2341 ttgccgcgcc gccggatgtc accggctcgg tcgtggcctc ggcgctgggg cggtggccct
     2401 cggccgtggt caccgatgtg gcctcggtca aggtcgccat cctgcgcgac gtcgtctcgc
     2461 ggagggcgga cctgagcagg tatgtcgggt cacaccccat ggcgggccgg gagcggtccg
     2521 ggaccatcgc cgcccggggt gacctgttcc agggtcgggc ctgggtcgtc gtgccgcacg
     2581 agggatccga cccggacgcg gtggcgctcg tggcgcgggt cgccaccgat accggcgcag
     2641 ccgtccggct catggacccc gagggtcacg atgccgcggt cgcggccgtc tctcacgtgc
     2701 ctcaggttgc ggcctcgctc gtggccgcgc ggctgcgcga cctcaccgag ccggcgctcg
     2761 cgttgtcagg gcagggcctg cgtgacgtga cgaggattgc ggcgagcgac gagcgcctct
     2821 ggacccagat cctggccggc aacgccggtg cggtccgcga ggtgctcgct gccctgcacg
     2881 ccgacctggg cgaggtcatt gccgcactcg ccaccgtcgg cacggccgag ggggtcggcg
     2941 ccctcgaggt gctggcccgg acgatcggca acggcaacct cgggtacgcc ctgatcccgg
     3001 gcaagcatgg ggccgcaccg acgacatacg cgatcgtcgc cgtcgtcgtc cccgacgagc
     3061 cgggcatgct cggccgcctg ctcgtcgccg tcggcgaggc gggggtcaac ctcgaggacc
     3121 tcgagctcga gcacggtctg ggcctgccct tcggcattgc cgagatcgcc gtgctgccgg
     3181 gatcggcgga gcatctggcc cgcgagctca cccggcgagg atggcgcctg cactcatgag
     3241 caccggactg gtggtcgcga tcgacggacc gtcggggtcg gggaagtcga gcgtctcgcg
     3301 ggccgtggcg tcgcggctcg gggtggggta tctcgacacc ggagcgatgt accgggcgct
     3361 cacgtggtgg tgcctcgagc ggcgcctcga cctcgacgac gccgacgccg tggctgcggc
     3421 cgcggcggcg ctgcccctcg tcatcggact cgacgcccag gcgccgacgg tcgaggtgga
     3481 cggcgccgat gtgggtgcgg ccatccgcga gacccggatc tccgaggcgg tgtctgccgt
     3541 cgccaccaac ctggccgtac gggcgaacct gcgggggcgc cagcgccgtc tcatcgccga
     3601 ggcggtggcc tctcgcgggg gggtcgtggc cgagggacgc gacatcacca ccgtcgtggc
     3661 tccggacgcc gacgtccggg tgctcctcac cgccagcgag gcggcgcggc tacagcggcg
     3721 cgcacgcgag ctgctggggt ccgccgatgc cgccagcctg gccgccattc acgcgcagat
     3781 cgtccaccgg gaccagcgcg actccaccgt ctcgcagttc accgaggccg cggacggagt
     3841 ggtcctggtt gacacgtccg gcctggacct cgacgggtcg gttgaggcgg tcctgcaggt
     3901 cgtcgccgcg gtctcgggcc ggcgtggcta ggaccggcac cgcggggcac ccggccgggt
     3961 ggcgtccacg ggttggccag gccatcgggc gggtgctgtt cggcgcaggc tatcgctgcc
     4021 ggacgctcgg cacggcccgc tccgtcctgc gggccggggg agtggccggc gtcttccccg
     4081 aagggacccg cggccgcggc gacgtggccc aggtcagcca gggcgccacg tggctcgccc
     4141 cgcacagcgg tgcccagatc gtgcccgtcg aggtccagct cggacgtgtc gacgaggacc
     4201 acaccgtcgg cggcctcggt gaactgcgag acggtggagt cgcgccggtc ccggcggacg
     4261 atctgctggc gggtggcggc aaggctggcg gcgtcggctg cgccgtgcag ctcacgggcg
     4321 cgccgcgcga gccgcgcggc ctcgctcgcg gtgaggagca c
//
LOCUS       DATAIX010000642         1552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_290150, whole genome shotgun sequence.
ACCESSION   DATAIX010000642 DATAIX010000000
VERSION     DATAIX010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290150"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1388
                     /locus_tag="VHM65_09480"
     CDS             129..1388
                     /locus_tag="VHM65_09480"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase domain-containing
                     protein"
                     /protein_id="HEX2705977.1"
                     /translation="MAFYQSVGSIPAKRHTQHRRPSGELYSEELMGEEGFSSDSSLLY
                     HRHLPSKVAAAMPWEVGDYSTVANHPLLPRHLRPHALFAAGAGADGAGVDVVTGRRLL
                     LGNSDVRLLYVVADTPSPWYRNGIGDECVYVERGAARVETVFGAFAAGRGDYLLIPRA
                     TTHRWLPVTAEQPGYAEPLRAIVVEASSHIAPPKRYLSRYGQLLEHAPYCERDLRAPV
                     GPLLAEDIGEDPDGPTDVHIKHRGSGPASSGGTLGTTYTYPFHPLDVVGWDGCLYPYA
                     FNVRDFEPITGRVHQPPPAHQVFEGASFVICNFVPRKVDYHPLAIPVPYYHSNVDSDE
                     VMFYVDGDYEARKGSGITTGSVSLHPGGHAHGPQPGATERSLGLEYFDELAVMVDTFR
                     GLDLGEAGRACDDGEYAWTWMKGRPVE"
BASE COUNT          233 a          547 c          541 g          231 t
ORIGIN      
        1 cagcgcgctc ggcgacgctc gttgacatgc atcgcaggct acttcatggt gggtgggacc
       61 gggtgaacga caggtgacga agcccgggag cggcgtgcgg gccgacccgc cacgcggaag
      121 gggcgacgat ggcgttctac cagtccgtgg gcagcatccc ggcgaagcgg cacacccagc
      181 accgccgccc gagcggtgag ctctactccg aggagctcat gggcgaggag ggcttctcct
      241 cggactcctc gctgctctac caccgccacc tgccctcgaa ggtggcggcg gcgatgccgt
      301 gggaggtggg cgactactcg accgtggcga accaccccct gctgccgcgg cacctgcgcc
      361 cgcacgcgct cttcgccgca ggcgctgggg cggacggggc tggcgtcgac gtcgtcaccg
      421 ggcggcggct gctgctcggc aacagcgacg tccggctgct ctatgtcgtc gccgacacgc
      481 caagcccgtg gtaccgcaac ggaatcggcg acgagtgcgt gtatgtcgag cgcggcgccg
      541 cgcgggtcga gacggtgttc ggggccttcg cggccgggcg aggcgactat ctcctcatcc
      601 cccgggcgac gacccaccgg tggctcccgg tcaccgcgga gcagcccggg tatgccgagc
      661 cgctgcgcgc catcgtcgtc gaggcgagct cacacatcgc cccgcccaag cgctacctgt
      721 cgcgctatgg ccagctcctg gagcacgcgc cctactgcga gcgcgacctg cgtgccccgg
      781 tgggaccgct gctggccgag gacatcggcg aggaccccga cgggccgacc gacgtccaca
      841 tcaagcaccg ggggagcggg ccggcctcct ccggcgggac cctcgggacg acatacacct
      901 atccgttcca cccgctcgac gtcgtcggct gggacggctg cctctacccc tacgcgttca
      961 acgtccgcga cttcgagccg atcaccggcc gggtccacca gccgccgccg gcgcaccagg
     1021 tcttcgaggg cgcgagcttc gtcatctgca acttcgtgcc gcgcaaggtc gactaccacc
     1081 cgctggcgat cccggttccc tactaccact ccaacgtcga ctccgacgag gtgatgttct
     1141 acgtcgacgg ggactacgag gcccgcaagg gctcgggcat cacgaccggg tcggtgtcgc
     1201 tgcaccccgg tggccatgcc catggtcccc agccgggagc caccgaacgc tcgctcgggc
     1261 tcgagtactt cgacgagctg gcggtcatgg tcgacacgtt cagggggctc gacctcggcg
     1321 aggccgggcg ggcgtgcgat gacggcgagt acgcctggac gtggatgaag ggccggcccg
     1381 ttgagtgagc tcaggtaggc tgtcgtaagt tcatgggcga caactcacgt atcctgatcg
     1441 tgtgcaccgg gaacgtctgc cggtcgccgt tcatcgagcg actcctgcag catctgctgg
     1501 acgagcgtca gcgtggagcg gagcgggcaa tcctggtgga gagcgcgggt ac
//
LOCUS       DATAIX010000643         2959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_256334, whole genome shotgun sequence.
ACCESSION   DATAIX010000643 DATAIX010000000
VERSION     DATAIX010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256334"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VHM65_09485"
     CDS             <1..555
                     /locus_tag="VHM65_09485"
                     /inference="COORDINATES: protein motif:HMM:NF016557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705978.1"
                     /translation="RRATLERLDLAGPHWTVPPTFPDGRSLHEATLVQGLEGVVSKRV
                     SSRYLPGRRSDDWRKNAHRTSRSLVIGGWRPEAERPGRLGAVLVGVPGPAGLRYRGRV
                     GAGLAGRAGEALLADLRAIERADNPFADDVPREDSTGTTWVAPIHVAEVSALGLSGSG
                     RLRQPAYRGLRPDLTAADVSDDDA"
     gene            548..1465
                     /gene="ligD"
                     /locus_tag="VHM65_09490"
     CDS             548..1465
                     /gene="ligD"
                     /locus_tag="VHM65_09490"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX2705979.1"
                     /translation="MPDLPAAKVPVVIDGHRIMLSSLDKVMYPATETTKGEVLNYYAR
                     VAPLILPHLAGRPVTRVRWPHGVEGPSFFEKNLPSGAPSWLTSVTLPSNSARGATDRD
                     SVTYPLVSDTAALTYLVNLNSLELHIPQWRVDAEGQPDNPDRLVIDLDPGSGAGLTEC
                     ARVALHVRGRLTRIGLDPIPVTSGSKGMQVYAALPGAHTSEDMRAVALQLAQDLTRAL
                     PDLVVWKMTKSLRPGRVLLDWSQNVAAKTTVCPYSLRGRELPAVAAPRTWDEVEHGAE
                     TPGALGQIGIDAALGRFERVGDLAAGLLR"
     gene            complement(1531..1860)
                     /locus_tag="VHM65_09495"
     CDS             complement(1531..1860)
                     /locus_tag="VHM65_09495"
                     /inference="COORDINATES: protein motif:HMM:NF023202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsr2 family protein"
                     /protein_id="HEX2705980.1"
                     /translation="MAQRTLVVLEDDIDKSPAADTVTFGLDGVSYEIDLSESNAAKLR
                     DALAPWVAHGRRSGGRKISRRGSAGGGKRPDLGLVRDWARNNGFKVSDRGRISAEVQA
                     AYDSASA"
     gene            complement(2058..2339)
                     /locus_tag="VHM65_09500"
     CDS             complement(2058..2339)
                     /locus_tag="VHM65_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705981.1"
                     /translation="MRIEMGTLWQDAFPYVAALLPTAGTVFLFYFVMKYIFEGDRRER
                     IAQAQWEAAHGGPRRTASPVNVPAESADLSEVSDDPGADAGESAGDSPA"
     gene            complement(2329..>2959)
                     /locus_tag="VHM65_09505"
     CDS             complement(2329..>2959)
                     /locus_tag="VHM65_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX2705982.1"
                     /translation="IRDLYLAAADAVGSRQVWTARGVVDLDGEWRWLPVYPAVSEAVG
                     EEVTLSTPVERLRELAATHGVALDPAWGADKVVLELLGELVEPALLQPTFLCDYPAIA
                     QPLARPHRREPGLIEAWDLVIAGIERGTGFSELVDPVVQREVLTAQSLLAAGGDDEAM
                     VLDEDFLRALEYGAPPMGGLGVGIDRMVMLFTGANIRETILFPIVKRED"
BASE COUNT          450 a         1083 c         1007 g          419 t
ORIGIN      
        1 cggcgcgcga cgctggaacg gctcgacctg gccggccccc actggaccgt gccaccgacc
       61 ttccccgacg gccgcagcct gcacgaggcc accttggtcc aaggactgga aggggtcgtc
      121 agcaagcggg tcagctcccg ctatcttccc ggccgacgca gtgacgactg gcgcaagaac
      181 gcccaccgga cctcgcgctc gctcgtcatc ggcggctggc gcccggaggc cgagcgtccc
      241 ggccggcttg gcgccgtcct cgtcggggtc cccgggcccg cagggctgcg ctaccggggc
      301 cgggtcggcg ccggcctggc cggccgggcg ggggaggcac tgctggccga cctgcgcgcg
      361 atcgagcggg ccgacaaccc gttcgccgac gacgtgcccc gcgaggactc gacgggcacc
      421 acctgggtcg cccccatcca cgtggccgag gtgagcgcgc tgggactgag cgggtcaggc
      481 cggctgcggc agccggccta ccggggcctg cgtcctgacc tcacggccgc agatgtgagc
      541 gacgacgatg cctgacctgc cggcggcgaa ggtgcccgtg gtcatcgacg ggcaccggat
      601 catgctctcc agcctcgaca aggtcatgta ccccgcgacc gagacgacca agggagaggt
      661 gctcaactac tacgcgcggg tcgcgccgct catcctgccc cacctcgccg ggcggccggt
      721 gactcgggtc cgctggccgc acggcgtcga ggggccgagc ttcttcgaga agaacctccc
      781 ctcgggcgct ccgtcgtggc tcacgtcagt gacgttgccc tccaacagcg ctcgcggggc
      841 caccgaccgc gactcggtga cttaccccct cgtcagcgac actgcagcgc tgacctacct
      901 cgtcaacctc aactcgctcg agctgcacat cccccagtgg cgcgtcgacg ccgaggggca
      961 gccggacaac cccgaccgcc tcgtcatcga cctcgacccc gggtccggcg ccggtctgac
     1021 cgagtgtgcc cgggtcgccc tgcacgtgcg cggccggctc acccggatcg gcctcgaccc
     1081 gatcccggtc acgagcggga gcaagggcat gcaggtgtat gcggcgctgc caggtgccca
     1141 cacgtcggag gacatgcggg cggtggcgct gcagctggcc caggacctga cccgggcgtt
     1201 gcccgacctg gtcgtctgga agatgacgaa gtcgctgcgc cccggcaggg tgctgctcga
     1261 ctggagccag aacgtcgccg ccaagacgac ggtgtgcccg tactcactgc gggggcgcga
     1321 gctgcccgca gtggccgccc cgcgcacctg ggacgaggtc gagcacggcg ccgagactcc
     1381 aggcgcgctc ggccagatcg gcatcgacgc ggcgctgggc cggttcgagc gcgtgggcga
     1441 cctcgctgca ggtctcctgc gctgacgcca ccgacagacg cgccggtggc gcgtctgtcg
     1501 gtggcgcagc tgagtgccgt gcggccgcgg tcaggcggat gcactgtcgt aggcggcctg
     1561 aacctcggcc gagatccggc cccggtcgct caccttgaag ccgttgttgc gcgcccagtc
     1621 ccggaccagg cccaggtcgg gcctcttgcc accaccggcc gacccccggc gggagatctt
     1681 gcggccgccc gagcggcgcc cgtgcgctac ccacggggcc agtgcgtcgc gcagctttgc
     1741 cgcgttgctc tcagaaaggt cgatctcgta gctgaccccg tcgagcccga aggtgacggt
     1801 gtcggccgcc ggggacttgt cgatgtcgtc ctcgagtacg accaacgtac gctgagccat
     1861 ttatgaactc ctagtcgatt gatggtgcgc ctgctcggat gaacggcgcc tcggcgatct
     1921 gacgtcacgt tgcagttagt gccctgctgc cacacgaccc gattcatcgt ggaatgcaca
     1981 ctaccacttg gtcaagagaa agggcttcac acacgcgagc acgggcctca tcacatcggc
     2041 gaggcggcgc cacgcattca cgccggggag tcgccagcac tctcgccggc gtcggcgccc
     2101 ggatcatcgg agacctcgga taggtcggca ctctcagcgg gcacgtttac gggagaagca
     2161 gtccggcgcg gcccaccgtg tgcggcctcc cactgggcct gggcgatccg ctcgcgccgg
     2221 tcgccctcga agatgtactt catgacaaag tagaagagga agaccgtccc agccgtgggc
     2281 aacagcgccg caacgtaggg gaacgcgtcc tgccagagtg tccccatttc aatcctcacg
     2341 cttgacgatc gggaagagga tggtctcccg gatattcgcg ccggtgaaga gcatgaccat
     2401 tcggtcaatg ccgacaccca atccacccat cggcggggcg ccgtactcca gtgcccggag
     2461 gaagtcctcg tccagcacca tggcctcgtc gtccccgccg gcggcgagca gcgactgggc
     2521 ggtgaggacc tcgcgctgga ccaccgggtc gacgagctcg gagaacccgg tgccgcgttc
     2581 gatgccggca atgacgaggt cccacgcctc gatgagccca ggctcgcgcc gatgcgggcg
     2641 ggccaagggc tgggcgatgg ctgggtagtc acacaggaac gtcggctgca gcagcgccgg
     2701 ctcgacgagc tcgccgagca gctcgaggac gaccttgtcg gctccccacg cggggtccag
     2761 cgcgacgccg tgcgtggctg cgagctcgcg cagccgctcg accggtgtcg agagcgtgac
     2821 ctcctcgccc accgcctcgc tcaccgccgg atagaccggc agccacctcc actcgccgtc
     2881 gaggtcgacc accccgcggg cggtccacac ctgccgcgac cccaccgcat cggccgccgc
     2941 gaggtacaga tccctgatc
//
LOCUS       DATAIX010000644         1872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_256344, whole genome shotgun sequence.
ACCESSION   DATAIX010000644 DATAIX010000000
VERSION     DATAIX010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256344"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..703)
                     /gene="purQ"
                     /locus_tag="VHM65_09510"
     CDS             complement(32..703)
                     /gene="purQ"
                     /locus_tag="VHM65_09510"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX2705983.1"
                     /translation="MRIGVVTFPGSLDDRDAARAVRFVGADAVGLWHADADLHGVDAV
                     ILPGGFSYGDYLRCGAIARFAPVMDVLIPAAQGGLPVLGICNGFQVLCESHLLPGALI
                     RNDHRTFSCVDQRLVVENTSTAWTSDFEPGQEVTIVLKNGEGGFVADTSTLDRLDGEG
                     LVVFRYAGRNPNGSYRDIAGITNERGNIVGLMPHPEHCVETGYGPSLDGVPFFTSVME
                     QVLAS"
     gene            complement(700..948)
                     /gene="purS"
                     /locus_tag="VHM65_09515"
     CDS             complement(700..948)
                     /gene="purS"
                     /locus_tag="VHM65_09515"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF014726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX2705984.1"
                     /translation="MGRIVVDVMLKPEILDPQGQAVGGALPRLGFAGFTEVRQGKRFE
                     LTVDGDVTAEHLAQARGAAEKLLSNPVIEDVVSIRAEQ"
     gene            complement(1097..1720)
                     /locus_tag="VHM65_09520"
     CDS             complement(1097..1720)
                     /locus_tag="VHM65_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705985.1"
                     /translation="MTEHPQGTPDAGLERFKVRQKLTLMVNRYEVLGVDAGGTETGVI
                     CFAEQKRMAFKEQVTFYSDASKSLVVFGFKARQVMDIGATYDVVDSAGRPIGWFKKDF
                     GKSLLRSTWHLGTSDGLTATGQERNQKVAILRRVWEMIPVIGEIPVPFLFHFDFLAPD
                     GSVVLSSVKKAGIKDVYSVEVPASGGRRLDWRVAAAMAVALDALQSR"
BASE COUNT          298 a          632 c          656 g          286 t
ORIGIN      
        1 gcggcgtgct cgacggtgtc gacctcggtc gtcatgaggc cagcacctgc tccatgacgg
       61 aggtgaagaa cggcaccccg tcgagcgacg gcccgtagcc ggtctcgacg cagtgctcgg
      121 ggtggggcat gagcccgacg atgttgccgc gctcgttcgt gattccggcg atgtcccggt
      181 aggagccgtt cgggttgcgc ccggcatacc ggaagacgac gagcccctcc ccgtcgaggc
      241 ggtcgagggt cgaggtgtcc gccacgaacc caccctcgcc gttcttgagt acgatcgtga
      301 cctcctggcc gggctcgaag tccgacgtcc aggcggtcga ggtgttctcg acgacaaggc
      361 gctggtcgac gcagctgaac gtgcggtggt cgttgcggat cagcgccccg ggcagcaggt
      421 gcgactcgca caggacctgg aagccgttgc agatccccag gaccggcaag ccgccttgtg
      481 ccgcggggat gaggacgtcc atgacgggcg cgaaccgggc gatggccccg cagcgcaggt
      541 agtcgccgta ggagaacccg cccggcagga tgaccgcgtc gaccccgtgc aggtcggcat
      601 cggcatgcca caggcccacg gcgtcggcgc cgacgaagcg aacggcccgg gccgcgtcgc
      661 ggtcgtcgag cgagcccggg aaggtgacga ccccgatcct cactgctcgg ccctgatgct
      721 gacgacgtcc tcgatcaccg ggttcgagag cagcttctcg gcggccccgc gggcctgcgc
      781 gaggtgctcg gcggtgacgt caccgtcgac ggtgagctcg aaccgcttgc cctggcgcac
      841 ctcggtgaac ccggcgaagc cgagccgggg cagcgcacca ccgacggctt gcccttgcgg
      901 gtcgaggatt tcgggtttga gcatgacgtc aacgacgatg cgtcccatca ggagatgctc
      961 cttgaactcg tcggtggccc ggcggctgtc acgggcccag ccggggcgga cacacccagc
     1021 ctagccatga ggtcgtatgt cggccgactc gcctcaccgt gcgggcgatg tgtcccaagt
     1081 ggcgggcgga cagcccttag cgggactgca gcgcgtcgag cgcgacggcc atcgcggcgg
     1141 cgacccgcca gtcgagccgg cgcccgcccg aggccgggac ctcgaccgag tagacgtcct
     1201 tgatccccgc cttcttgaca ctgctcagga cgaccgaccc gtccggagcg aggaagtcga
     1261 agtggaagag gaacggcacg gggatctcgc cgatgaccgg gatcatctcc cagacccggc
     1321 gcaggatcgc caccttctgg ttgcgctcct gcccggtcgc ggtgaggccg tccgaggtgc
     1381 cgaggtgcca cgtcgagcgc agcagcgact tgccgaagtc cttcttgaac cagccgatcg
     1441 ggcgaccagc ggagtccacg acgtcatacg tcgcaccgat gtccatgacc tggcgtgcct
     1501 tgaagccgaa gacgaccagc gacttggacg cgtcggagta gaaggtgacc tgttccttga
     1561 aggccatccg cttctgctcg gcgaagcaga tcacgccggt ctcggtgccg ccggcgtcga
     1621 cgccgaggac ctcgtagcgg ttgaccatga gggtgagctt ctggcgcacc ttgaaccgct
     1681 cgagcccggc gtccggggtc ccctgggggt gttcagtcat accgacactg tgtcatgcga
     1741 caactgtgcc atggcgacca ccgtgtcacg gacccgcgct cagccgaagg actggccggt
     1801 cagcagctcg taggcctcga cgtacttggc cctcgtccgg tccacgacct cctggggcag
     1861 cgccgggggg gg
//
LOCUS       DATAIX010000645         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_230220, whole genome shotgun sequence.
ACCESSION   DATAIX010000645 DATAIX010000000
VERSION     DATAIX010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230220"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VHM65_09525"
     CDS             complement(<1..209)
                     /locus_tag="VHM65_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705986.1"
                     /translation="MRPDEAAGVARLVGLALKGTSTLVYDVQAVASERVFDALAVAGG
                     PLVSPVRVVHELSTAGVYAAVRGGL"
     gene            complement(286..945)
                     /locus_tag="VHM65_09530"
     CDS             complement(286..945)
                     /locus_tag="VHM65_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX2705987.1"
                     /translation="MSVHQVLLFAAAAAVLIAIPGPNHIYIATRSIADGRHAGLASAL
                     GVEAGTLVHTALAAAGLSYLIVTSATAFAVVRYAGAAYLVYLGITTLRRRRGSVQTWG
                     GSAPPRPWRAFRDGVVVNMLNPKVILFFLAFVPQFVDLDAGSVPMQIVVFGAVTMTIG
                     LTTSIGYVLAAGALRRRLARSPRLVSGQRFVVGPIYLLLGLATALSGASGSSRAALVP
                     R"
     gene            complement(942..1349)
                     /locus_tag="VHM65_09535"
     CDS             complement(942..1349)
                     /locus_tag="VHM65_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705988.1"
                     /translation="MPIPYVVTAFTHHLLNPLVLRAAGRLSVGDLEHVGRSSGRVFHT
                     PIMAFRESDVVTIALTYGTDVDWYRNLRAAGGGRLRLGGSLLRLGPPRPIGVDSAMAR
                     IPHPQKAILPIIRCRDFIELRVLGETPVDRGAA"
     gene            complement(1495..>2132)
                     /locus_tag="VHM65_09540"
     CDS             complement(1495..>2132)
                     /locus_tag="VHM65_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705989.1"
                     /translation="AVSIEYPRKNLAIHDIVPFRTQVLSDLIVHIKEQTEYDRLKLVL
                     IGYSRGTVPSRHAATEQSRYIDGLSLIAPTWFREVVRPRELAQKGLAESARGIMRESW
                     VDKWGLISAGARLAQEMLTHPIALRNDVASISEEGAADLEQVLTRGVRVGVVAGLRDE
                     LCEVDGIRQVLGALSQPLAVDYREVESDHFYYFVKPSSLKVVAEQVTRLAG"
BASE COUNT          354 a          737 c          752 g          289 t
ORIGIN      
        1 tcgagtccgc cccgcacggc ggcatagaca ccggccgtgc tcagctcgtg gacgacccga
       61 accggcgaca cgagtgggcc accagccacc gcgagcgcgt cgaacacccg ctcgctcgcc
      121 accgcttgga cgtcatacac gagagtgctc gtgcccttga gagccagccc cacgagccga
      181 gccactcccg ctgcctcgtc cggtcgcatc tcgcccgacc tccccgcgcg ttgcctccgt
      241 ctgggtaacc tacgctaccg taagttaccc gccggaccta ggtgctcagc gcgggaccag
      301 ggccgcgcgg gaggatcccg aggcgccgct gagcgcggtg gccaggccga gcagcaggta
      361 gatcggaccg acgacgaagc gctgccccga gaccaggcgc gggctccggg cgagccgccg
      421 cctcagcgcc ccggccgcca gcacgtaccc tatgctcgtc gtcagcccga tcgtcatggt
      481 gaccgcgccg aagacgacga tctgcatcgg caccgacccc gcgtcgaggt cgacgaactg
      541 gggcacgaag gcgaggaaga agaggatcac cttggggttg agcatgttga cgacgacccc
      601 gtcacggaag gcccgccacg ggcgcggggg cgcggacccg ccccatgtct ggaccgagcc
      661 gcggcgtcgg cgaagcgtcg tgatcccgag atagaccagg taggccgcgc ccgcgtaccg
      721 caccaccgca aaggccgtgg ccgacgtcac gatgaggtag gacagcccgg ccgccgcgag
      781 cgccgtgtgg accagggtac ccgcctcgac gccgagcgcc gaggccagcc cggcgtggcg
      841 gccgtcggcg atgctgcggg tcgcgatgta gatgtggttc ggcccaggga tcgcgatgag
      901 caccgccgcc gcggccgcga agagcaggac ctggtggaca ctcacgcggc gccccgatcc
      961 acgggagtct ccccgagcac gcgcagctcg atgaagtccc ggcagcggat gatgggcaga
     1021 atcgccttct gcgggtgggg gatccgagcc atggcgctgt ccacgccgat cggccggggt
     1081 ggtccgagcc gcagcaggct gcccccgagc cgcagccgcc ctccgccggc tgctcgcagg
     1141 ttgcggtacc agtcgacatc ggtcccgtag gtgagggcga tcgtgaccac atcgctctcg
     1201 cggaacgcca tgatcggggt gtggaagacc cggcccgacg agcggccgac atgctcgagg
     1261 tcaccgaccg acaagcgccc tgccgcgcgc aggacgagtg ggttgagcag gtggtgggtg
     1321 aacgcggtca cgacgtacgg aatgggcacc cctgttgtcc tctcctgcgc cggcagcacc
     1381 tgaccggcca tttcgcggac cggcattccc ggatacgacc gatcgtgcca cgtgttgcgt
     1441 gtactgggtg cagcacacgc aacacggcgc gaccgcaacg gagcgcaccg tgggtcagcc
     1501 ggcgaggcgg gtgacctgtt cggcgacgac cttgagcgag ctgggcttga cgaagtagta
     1561 gaaatggtcc gactcgacct cgcggtagtc gaccgccaag ggctgggaga gcgcaccgag
     1621 gacctggcgg atgccatcga cctcgcacag ctcgtccctg agcccggcga cgacaccgac
     1681 ccggacacct ctggtcagga cctgctcaag gtcggcggcg ccctcctcgg agatcgaggc
     1741 gacgtcgttg cgcagcgcga tcgggtgggt cagcatctcc tgggcgagcc gggcaccggc
     1801 cgagatgaga ccccacttgt cgacccagct ctcgcgcatg atgccccggg cgctctcggc
     1861 gaggcccttc tgggccagct cgcgaggccg gaccacctcc cggaaccacg tcggggcaat
     1921 gaggctgagc ccatcgatgt agcgggactg ctcggtggcc gcgtgccggc taggcaccgt
     1981 cccgcgcgag tagccgatca ggacgagctt gagccggtcg tactcggtct gttccttgat
     2041 gtggacgatg aggtcgctca ggacctgggt gcggaagggc acgatgtcat ggatggcgag
     2101 gttcttgcgg gggtactcga tcgagacggc ca
//
LOCUS       DATAIX010000646         1788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_200412, whole genome shotgun sequence.
ACCESSION   DATAIX010000646 DATAIX010000000
VERSION     DATAIX010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200412"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VHM65_09545"
     CDS             <1..768
                     /locus_tag="VHM65_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705990.1"
                     /translation="SLRRRVVGLRPAVTGEPILRFETGPGIQTQGDWADVGAWPLGEG
                     TADLFALVAILGCSRMVAFRFATDKTRPTTLRSIVRCVDDLGGATAEFLTDRDTALMA
                     GSRADGAPIYAPEWLDVAAMLGTRPRACRPYRAKTKGKVERVIREIKEDFLAWLTGQV
                     LPEFPTLAWYDAAARRWATGVHATRRHRTTGRVIGEAWADERPLLLPVSGRLIGRMEG
                     QDTLVPLPAPVSASRMPAALGETVEVRPLAVYAELAR"
     gene            765..1559
                     /gene="istB"
                     /locus_tag="VHM65_09550"
     CDS             765..1559
                     /gene="istB"
                     /locus_tag="VHM65_09550"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX2705991.1"
                     /translation="MSRGAESRVATAYARTREHLAYLGLTTASERLAAELERASTETA
                     APVEVLERLLAAEVEATAARRLSGRLRFAHYPLDKRLAQFEFDFQPSIDRAVIAELST
                     LRFVEERRNALFLGPPGVGKSHLAIALGIAATEAGYRTYFTTAVDLVAHLQAAHLEGS
                     WSAKMRTYTGPSVLVIDELGYLPMDATSAHWIFQVVSRRYERGSIVLTSNRGFGDWGQ
                     VFADQVVASAILDRLLHHATVVNIKGQSYRMRAHVPSPREGAAMLG"
BASE COUNT          236 a          664 c          639 g          249 t
ORIGIN      
        1 agcctgcgcc ggcgggtcgt cgggctccgg ccggcggtga cgggcgaacc catcctgcgc
       61 ttcgagaccg ggcccggcat ccagacccag ggcgactggg ccgacgtggg ggcctggccg
      121 ctcggcgagg ggacggccga cctgttcgcc ctggtggcca tcctcggctg ctcgcggatg
      181 gtcgccttcc gcttcgcgac ggacaagacg cgcccgacga ccctccgttc gatcgtccgc
      241 tgcgtcgatg acctcggcgg ggcgaccgcg gagttcctga ccgaccggga cacggcgctc
      301 atggcgggct cccgcgccga cggcgccccg atctacgccc ccgagtggct cgatgtcgcc
      361 gcgatgctcg ggacgaggcc acgggcgtgt cggccgtacc gggccaagac caagggcaag
      421 gtcgagcggg tgatccggga gatcaaggag gacttcctcg cctggctcac cgggcaggtg
      481 ctcccggagt tcccgacgct cgcctggtac gacgccgccg cccggcgctg ggccaccggg
      541 gtccatgcga cgcggcgcca ccggacgacc gggcgggtca tcggcgaggc gtgggccgac
      601 gagcggccgc tcctgctgcc cgtcagcggc cgcctgatcg ggcggatgga gggccaggac
      661 accctcgtgc ccctgccggc ccccgtgagc gcctccagga tgcccgctgc gctcggcgag
      721 accgtcgagg tccggcccct cgcggtctat gccgagctgg cccgatgagc cggggcgcgg
      781 agtcccgggt cgcgaccgcg tatgcccgga cccgtgagca cctcgcctac ctcggcctca
      841 cgacggcgtc cgagcgcctc gcggccgagc tggagcgggc cagcaccgag acggcggccc
      901 cggtcgaggt cctcgagcgg ctcctcgcgg ccgaggtgga ggcgacggct gcccgccggc
      961 tgtcgggcag gctgcgcttc gcccactacc cgctcgacaa gcgcctcgcg cagttcgagt
     1021 tcgacttcca gccctcgatc gaccgggccg tcatcgccga gctgtccacc ctgcgcttcg
     1081 tcgaggagcg gagaaacgcg ctgttcctcg ggccacccgg ggtcggcaag agccacctcg
     1141 cgatcgcgct cgggatcgcc gccaccgagg ccggctaccg gacctacttc acgaccgcgg
     1201 tcgacctcgt ggcccacctc caggcagccc acctcgaggg gagctggagc gcgaagatgc
     1261 ggacgtacac ggggccctcg gtgctcgtca tcgacgagct cggctacctg ccgatggatg
     1321 ccacgtcggc ccactggatc ttccaggtcg tgtcgcgccg ctatgagcgc ggctcgatcg
     1381 tgctgaccag caaccgtggc ttcggcgact ggggccaggt cttcgccgac caggtcgtcg
     1441 cgagcgccat cctcgatcgg ctcctgcacc acgccaccgt ggtcaacatc aagggccaga
     1501 gctatcggat gcgggcccac gtgccgtcac cgcgggaggg cgctgctatg ctcggctgac
     1561 gctgatcgcc tctgcacttt tcgtggcgat ctctctgcac gttccgtgct cacccacacc
     1621 gtgtgacggc ggtaccagtc tgtcaccgat caacaagctg agccgcgagc cgggctgtgc
     1681 cgcctgcgag ccgcgagcgg cgagccgagc cgggcgccgg gagccgggct gtgccgcctg
     1741 cgagacgcga gccgagccgg gagccgggag ccgggagccg agccattt
//
LOCUS       DATAIX010000647         1762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_290645, whole genome shotgun sequence.
ACCESSION   DATAIX010000647 DATAIX010000000
VERSION     DATAIX010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290645"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..388
                     /locus_tag="VHM65_09555"
     CDS             101..388
                     /locus_tag="VHM65_09555"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEX2705992.1"
                     /translation="MTFCQLDRTVVELTPAQVGEFAGNTLELTGGDGSVLVMSTRARR
                     SLTPGQLAVVESAAAVLALQVPTIELAGGSVRCMLAGIHMPPRSAQSVPER"
     gene            501..>1762
                     /locus_tag="VHM65_09560"
     CDS             501..>1762
                     /locus_tag="VHM65_09560"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2705993.1"
                     /translation="MRAAALIAAVAAAPIAAVAAAGPGMPGRLAFAAPAGMTPQLTSV
                     SGAGVGVSSARVAVGPEPDGSAVDLDVSVFTPGTGGRQPAVLLAHGFGGSKADLSVRA
                     TDLASHGYVVLTYTARGFGASGGRVHLDDPAFEVADARRLIDLLAGRPDVRLDGPGDP
                     KVAIIGTSYGGALALMTAGTDRRVDALVPAITWNDLSAAFFPQSAAGPQGAPTPAGTA
                     PIGRPGPLKALWASSFFQSPLVSPGPAVASGAGVSPDILCGRFDPVLCRLFTTALQTG
                     RSSDELLTALRAHSPAPSLGAVRAPTLLIQGMADSLFGLDQADATARALAAAGTPVAV
                     RWIDGGHDGASSTATADDAAAQAWVDHYLRGATPTSALAVPAFVYADPIPRRSSAAPL
                     RSLAAYPGLGAAPSLTRVALPLKAAEPQT"
BASE COUNT          212 a          666 c          621 g          263 t
ORIGIN      
        1 ccatagtgca acctgtatga ccgcgcaacc tgtatgaccg cgcaacctgt atgaccctgc
       61 taggcagact gttcgacaac ccagcagatc gagcactgct ctgacgttct gccagctaga
      121 ccgcaccgtc gtcgagctca ctcccgctca ggtcggcgag ttcgcgggca acacgctcga
      181 gctcacggga ggggacggat cggttctcgt catgtcgacg cgagcgcgcc ggtccctgac
      241 acccgggcag ctcgccgtcg tcgagtcggc tgctgcggtg ctggccctgc aggtgccgac
      301 gatcgagctg gccggagggt cggtccggtg catgctcgcg gggatccaca tgccgccccg
      361 gtcggcgcag tccgtgcctg agcgctgacc acgccagggc cgcgcacccc ggtgttggcc
      421 gccgtcccgt ggacagggtg agatagggcc atgacggcga gccctgcccc cggtggcacc
      481 cggtgaggcg gggtgctcgc atgcgcgccg ccgcgctcat cgcagcggtc gctgcagcgc
      541 ccatcgcggc ggtcgcagca gccggtcccg gtatgccggg tcggctcgcc ttcgcagccc
      601 cggccggtat gacgccacag ctcacctcgg tgtctggcgc gggcgtaggg gtcagctccg
      661 cccgggttgc ggtggggccg gagccggacg gctcggccgt cgacctcgat gtgtcggtct
      721 tcactcccgg cacgggtggg cgccagccgg ccgtgctgct cgcgcacggg ttcgggggca
      781 gcaaggccga cctgtcggtg cgagcgaccg acctggcctc ccacggctat gtcgtcctta
      841 cctacaccgc acggggtttc ggcgcctcag ggggacgggt ccacctcgac gaccccgcgt
      901 tcgaggtggc cgatgcgcgg cggctcatcg acctgctggc cggccggccc gatgtccggc
      961 tcgacggccc gggtgacccc aaggtcgcga tcatcggcac gtcatacgga ggagccctgg
     1021 ccctgatgac cgcggggacg gaccggcgcg tcgacgcact tgtcccggcg atcacgtgga
     1081 acgacctgtc ggccgccttc ttcccgcagt ctgccgccgg gccgcagggc gcgcccacgc
     1141 ccgccgggac ggcgccgatc gggcgccccg gccccctcaa ggcgctctgg gcgtcctcgt
     1201 tcttccagtc cccgctcgtc tcgccgggcc cggccgtcgc ctcgggggcc ggggtgtcgc
     1261 ccgacatcct gtgcgggcgg ttcgacccgg tgctgtgccg gctcttcacc acggccctgc
     1321 agaccgggag atccagcgac gagctgctca ctgcgctccg tgcccatagc ccggccccgt
     1381 cactcggtgc ggtccgggct ccgaccttgc tcatccaggg catggccgac tcgctcttcg
     1441 ggctcgacca ggccgatgcg acggcgcgcg cgctcgccgc cgctgggacc cctgtggcgg
     1501 tccgctggat cgacggtggt catgacgggg ccagctccac ggcgacggcg gacgatgccg
     1561 cggcccaggc gtgggtcgac cactacctgc gcggggcaac gcccacgagc gcgctcgccg
     1621 tgccggcgtt cgtctatgcc gacccgatcc cccggcggtc cagtgccgca ccactgcgga
     1681 gcctggcggc ctaccccggg ctcggagccg caccgagcct gacccgggtc gccctgccac
     1741 tcaaggcggc tgagccgcag ac
//
LOCUS       DATAIX010000648         1818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_256532, whole genome shotgun sequence.
ACCESSION   DATAIX010000648 DATAIX010000000
VERSION     DATAIX010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256532"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1370)
                     /locus_tag="VHM65_09565"
     CDS             complement(135..1370)
                     /locus_tag="VHM65_09565"
                     /inference="COORDINATES: protein motif:HMM:NF024732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX2705994.1"
                     /translation="MTPAQITSALREMLTTLKQPRVVASGNAATPWQLLAIVDEALPE
                     YRLNMLNAHAGLTWREGVVHETSFVGAGMRKSPGLAYVPARLSLVPRLFRQALFPDAV
                     ILHTSAPVNGKVSMGIEINVLAAAVEACKRRGGLVFAQVNPRMPYTFGDGEIAVEDLD
                     GYLEVDTPVALPVPDANRPVDETAQRIGEYCANRVSSGATLQMGIGAVPDATLPRLTK
                     LRGLGIWSEMFSDGLLVLQRSGALDPDRYVVASFAMGTEELLHWMHLNPQLKMVRTEV
                     TNDPPQIARQPLMTSINTALQVDLFDQANASRRRGRLYSGFGGQTDFVVGAIHSRGGQ
                     SMMALRSWHPKANVSTIVPLIEEPVTSFQHTVVITDQGVAELYGRSEKDQAAALIENT
                     AHPSVRAELWEEAAELGLA"
     gene            1486..>1818
                     /locus_tag="VHM65_09570"
     CDS             1486..>1818
                     /locus_tag="VHM65_09570"
                     /inference="COORDINATES: protein motif:HMM:NF027587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4916 domain-containing protein"
                     /protein_id="HEX2705995.1"
                     /translation="MTETENRGGWLSREELRDARERLPIVYVDLVPVRVDDRSTVTSV
                     GLLLRATDGQIRRELVSGRVLYHERIRDAIIRHVEKDLGPVSLPQVPLSPQPFTIAEY
                     FPTPGVTPF"
BASE COUNT          282 a          636 c          634 g          266 t
ORIGIN      
        1 cctcggcagg cgcacaaccc cggcggacgt cgaccgggcc gttcagatcc tggcagcagc
       61 atcgagggac tgatcggccc gggacgacac ggccagcctc gccatacacg cggctgaggg
      121 tggccgtggc cgcctcaggc caggccgagc tcggcggctt cctcccacag ctccgcgcgg
      181 acgctggggt gagcagtgtt ctcgatgagc gcggcagcct ggtccttctc gctgcgcccg
      241 tagagctcgg ccactccctg gtcggtgatg accacggtgt gctgaaacga ggtgaccggc
      301 tcctcgatga gcggcacgat cgtcgacacg ttggccttgg gatgccacga gcgcagcgcc
      361 atcatcgact ggccgccccg cgagtggatg gccccgacga cgaagtccgt ctgcccgccg
      421 aagcccgagt agagccggcc ccgccgccgc gacgcgttgg cctggtcgaa caggtcaacc
      481 tgcagcgcgg tgttgatcga ggtcatgagc ggctgacggg cgatctgcgg cgggtcgttc
      541 gtgacctcgg tgcggaccat cttgagctga gggttgaggt gcatccagtg aagcagctcc
      601 tcggtgccca tcgcgaacga ggcgacgaca taccggtccg ggtcgagggc cccgctgcgc
      661 tggagcacga gcagcccgtc ggagaacatc tccgaccaga tgccgaggcc ccgcagcttg
      721 gtcaggcggg gcagcgtcgc atcgggcacc gcgccgatcc ccatctgcaa ggtggcgccg
      781 gaggacaccc ggttggcgca gtactccccg atccgctggg cggtctcgtc gaccggccgg
      841 ttcgcgtcgg ggaccggcag ggcgacgggc gtgtccacct cgaggtagcc gtcgaggtcc
      901 tcgaccgcga tctcgccgtc gccgaaggtg tagggcatcc gcgggttgac ctgggcgaag
      961 acgagccccc cgcggcgctt gcatgcctcg accgccgccg ccaggacgtt gatctcgatc
     1021 cccatcgaca ccttgccgtt gaccggcgcg gaggtgtgca ggatcacggc gtccgggaac
     1081 agcgcctggc ggaacagccg tggcaccagg gagagccgcg ccggaacata ggcaagccca
     1141 gggctcttgc gcatcccggc accgacgaac gatgtctcgt ggacgactcc ttcgcgccag
     1201 gtcagcccgg catgggcgtt gagcatgttg agccggtact cgggcagcgc ctcgtcgacg
     1261 atcgcgagca gctgccacgg ggtcgcggcg ttgccgctcg ccaccacccg aggctgtttg
     1321 agggtggtga gcatctcgcg cagagcggag gtgatctggg caggcgtcat cgaccgactc
     1381 gaactcacgg ggtccaggct gccacaacac acggccgccg tggggaacga ggtcccgcgg
     1441 gtggcgcgcc cccctggtag aaacgaccct gtgacaaacg accccgtgac cgagaccgag
     1501 aaccgaggtg gctggctgag ccgcgaggag ctgcgtgacg cccgcgagcg cctccccatc
     1561 gtgtatgtcg accttgttcc cgtccgggtc gacgaccgca gcacggtgac cagcgtcggc
     1621 ctgctgctgc gcgcgaccga cggccagatc cgccgcgagc tcgtctcggg ccgggtgctc
     1681 taccacgagc gcatccgcga cgcgatcatc cggcacgtcg agaaggacct gggaccggtc
     1741 tcgctccccc aggtgcccct gtcgccgcag cccttcacga tcgccgagta cttccccacc
     1801 ccgggggtga cgcccttc
//
LOCUS       DATAIX010000649         2053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_200618, whole genome shotgun sequence.
ACCESSION   DATAIX010000649 DATAIX010000000
VERSION     DATAIX010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200618"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="VHM65_09575"
     CDS             <1..1141
                     /locus_tag="VHM65_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2705996.1"
                     /translation="TLTFEATLSTSFPNGTTDLPNAVVVVGTGSNCVPTAEQDADCTT
                     DTTVEAAPNIHAVKTVDGEHVTTANPGDVLHYAITVTNSGDAAGTADVTDDVSAILAH
                     ATIGAISDGGTLTAGVITWPQFSLAALTGTKTLTFEATLSTSFPNGTTDLPNAVVVVG
                     TGSNCVPTAEQDADCTTDTSVSEYKLTIDKTNDAPLVTLVLPDNTTADLPTADEGTTV
                     TYTLTYAVGKLSVHNGVITDVLPAGIQYVAGSATSNDEFTFESYDSTTRTLSWTAATV
                     TKGGSVTYKALVLTGASALSQPLTNVATIDSAQTVPASDTSDIFVPVIPKGETAPPTD
                     ILVAPDGTNSPGSSLMLILVVLGLLVVSIGLITPVPAVVRRRNQR"
     gene            complement(1297..>2053)
                     /locus_tag="VHM65_09580"
     CDS             complement(1297..>2053)
                     /locus_tag="VHM65_09580"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2705997.1"
                     /translation="SVIGAGPAYTDRPGATGAAYLVRGGGASLLLDLGQGSFPGLAGL
                     IDPATLEAVVISHLHPDHFIDLIPLRHYLRWEEPRRRVRVIGPGALSGRLDALYGEPD
                     FCAASLDVEALVEGRQTVGPFDLEVARVRHTESSFGFRISLAHVPDIPDAPGLVYSGD
                     CGHAADLDALARPGDTLLCEVSFGPGPVVHGAEHLDGPTVGSLAQRVDAGRVLLTHLQ
                     MGFDRAATVTSVRARFDGPVDLVDPGFETTIGS"
BASE COUNT          372 a          753 c          647 g          281 t
ORIGIN      
        1 gaccctcacc ttcgaggcga ccctcagcac gtccttcccg aacggcacga cggacctgcc
       61 caacgcggtc gtcgtcgtcg gcacgggcag caactgcgtc ccgaccgcgg agcaggatgc
      121 tgactgcacg accgacacca cggtcgaggc tgcaccgaac atccacgccg tgaagacggt
      181 cgacggcgag cacgtcacga ccgccaaccc gggcgatgtc ctccactacg ccatcaccgt
      241 caccaacagc ggtgacgccg ccggcacggc cgacgtgacc gacgacgtca gcgcgatcct
      301 cgcccacgcc acgatcggtg ccatcagcga tggcggcacc ctgacggccg gcgtcatcac
      361 ctggccacag ttcagcctgg ccgccctgac cgggaccaag accctcacct tcgaggcgac
      421 cctcagcacg tccttcccga acggcacgac ggacctgccc aacgcggtcg tcgtcgtcgg
      481 cacgggcagc aactgcgtcc cgaccgcgga gcaggatgct gactgcacga ccgacacgag
      541 tgtcagcgag tacaaactca ccatcgacaa gaccaacgac gcaccgctcg tgaccctcgt
      601 tctcccggat aacacgaccg cagatctgcc gaccgcagac gaaggcacca cggtcacgta
      661 caccctgacc tacgcggtcg ggaagctcag cgtgcacaac ggggtcatca cggacgtcct
      721 gcccgcaggc atccagtacg tggctggatc tgcgaccagc aatgacgagt tcaccttcga
      781 aagctacgac tcgacgaccc ggaccctgtc ctggacggcg gcgacggtca cgaagggcgg
      841 ctccgtcacc tacaaggcat tggtcctgac cggtgccagt gcactcagcc agccccttac
      901 caacgtcgcc acgatcgact cggctcagac cgtgccggcc agcgacacct cggacatctt
      961 cgttccggtc atccccaagg gggagacggc cccgccgacc gacatcctcg tggcgccgga
     1021 cgggacgaac agcccaggct ccagcctgat gctcatcctg gtcgtcctcg gactgctcgt
     1081 cgtgagcatc ggcctgatca ccccggtccc ggcagtcgtc cgccgccgga accagcgcta
     1141 aggctcacag cccagccgat cgtccgatct ccctccgggg aggtctggcg gtcggctggt
     1201 tcgttccgcc tccccattca tacgccgcga ccccgagcgg cgtccgaccg gggcgctcca
     1261 tcgcttatcg gtggggtgcc tcggtcgctt tcgtgttcag gacccgatcg tggtctcgaa
     1321 ccccggatcc acgaggtcca ccggaccgtc gaagcgggcg cgcacgctgg tgacggtggc
     1381 cgcgcgatca aagcccatct ggaggtgcgt caggagcaca cgccccgcat ccacgcgctg
     1441 cgcgagcgac cctaccgttg gaccgtcgag gtgctcggcg ccgtgaacca ccggacccgg
     1501 gccgaatgag acctcgcaca gcagggtatc gccggggcgg gcgagcgcgt cgagatccgc
     1561 cgcgtggccg caatcgcctg aatagacgag tccgggcgcg tcggggatat cggggacatg
     1621 ggcgagcgag atacgaaagc cgaaactcga ctccgtatgc cggacccgcg cgacctcgag
     1681 gtcgaacggg ccgacggtct gccgcccttc gaccagggct tcgacatcca gcgacgcggc
     1741 gcagaagtca ggttcgccgt agagcgcgtc cagcctcccc gagagtgccc ccgggccgat
     1801 gacccggacg cgtcggcgtg gttcctccca tcgcaggtag tggcgcagtg ggatgaggtc
     1861 gatgaaatgg tcgggatgga ggtggctgat gacgaccgcc tcgagcgtcg cggggtcgat
     1921 gaggcccgcc aggccgggga acgagccctg accgagatcg agcagcaggc tcgccccacc
     1981 gccccgcacg agatatgccg ccccggtcgc gccgggccga tcggtgtagg ccggaccggc
     2041 accgatgaca ctc
//
LOCUS       DATAIX010000650         2761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_290920, whole genome shotgun sequence.
ACCESSION   DATAIX010000650 DATAIX010000000
VERSION     DATAIX010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290920"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VHM65_09585"
     CDS             complement(<1..428)
                     /locus_tag="VHM65_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2705998.1"
                     /translation="MAKRPLTGTTFDTVIIGAGSAGSALASRLSKDPSRQVLVLEAGR
                     MDSWWDVLIHMPAALPFPIGNRFYDWRYESEPEPNMNGRRIYHARGKVVGGSSSINGM
                     IFQRGNPMDFERWGREPGMATWDYAHCLPYFNRMETCLAAA"
     gene            648..1430
                     /locus_tag="VHM65_09590"
     CDS             648..1430
                     /locus_tag="VHM65_09590"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2705999.1"
                     /translation="MKLVEGLFGWVDLATSDVEAARAFYTGLFGWEGTDVPTPMGPVY
                     TMFRKDAKLVAGMGPQPPGMADAGVPSVWNSYLMAPDVDATVAAVEVAGGTVVMPAMD
                     IMTQGRMAMVADPSGAVVGLWQPRDHEGAELFNVPGSLTWNELQTRDLAAATAFYAEV
                     FGWRWEPMEGSDYLVANIDAKEGEDKSNGGAMTMPDMVPAEAPSMWNVYFAVTDCDES
                     VTRAVELGGSVFLPAMTMGPGRFAGITDPTGAMFFLGAFPTA"
     gene            1581..2177
                     /locus_tag="VHM65_09595"
     CDS             1581..2177
                     /locus_tag="VHM65_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2706000.1"
                     /translation="MTNPYGDVELGTQGMRALAHPVRLAILIQLQNHGPKTATALAPA
                     VGASPSVTSWHLRHLAKHGLVRDSDVGTSGRERWWEAASRGFRFTAEDEAGREAQRLL
                     SAVLEEVEGDLPGQWTADVEPRLEPQWRSAAGRSNTRVLVTLEELAELESAIEALLAP
                     YVLRKDAGTDTIPAQARGVRLLRYVLPELPEPTGDASS"
     gene            2174..2758
                     /locus_tag="VHM65_09600"
     CDS             2174..2758
                     /locus_tag="VHM65_09600"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2706001.1"
                     /translation="MRDPDARRGMPSTAPLWRDRRFSTYWGGQTVSQLGDRVSELALP
                     LIAVTMLHASPTAVGLLTAAVWAPNLLSLFVGAWVDRQQRKRRLLIWGDLLQCVVIFS
                     LPVAYYLGTITFVHLVAVALLAARGPSSITRHTQRSSSPSFDETNISRRTPFSAGRVR
                     SRSSSARPSPVASFKRSPHRSPSSSTGCPSSSQP"
BASE COUNT          395 a          963 c          962 g          441 t
ORIGIN      
        1 gcggcggcca ggcacgtctc catccggttg aagtagggca ggcagtgcgc gtagtcccag
       61 gtcgccatac cgggctcgcg tccccagcgc tcgaagtcca tcgggttgcc ccgctggaag
      121 atcatgccgt tgatcgagct cgacccgccg accaccttgc cgcgcgcgtg gtagatccgt
      181 cggccgttca tgttcggctc gggctcggac tcgtacctcc agtcgtagaa acggttcccg
      241 atcgggaagg gcagagcggc cggcatgtgg atgaggacgt cccaccacga gtccatccgc
      301 ccggcctcga ggacgagcac ctgacggctc gggtccttgc tgagccggct cgccagcgcg
      361 ctgccggccg atcccgcgcc gatgatgacc gtgtcgaacg tcgtgccggt cagggggcgc
      421 ttggccatgt cggtctccgt tctgcggtcg ccttcgggac gatcgagact agccgcgcca
      481 ctgcgccacg agcaacgagt cgcgtatggc gcaactcccg gccgcgggtg caggctgtgc
      541 cggccccggg atcgccgggt cgtggacggt gtcatacctc gggcgtagcc tcgcgcacac
      601 gagacaccta ctcaccacga gacacctact cacgacagga gatgcgaatg aagcttgttg
      661 agggcctgtt cggctgggtc gacctggcca cgagcgacgt cgaggccgcc cgggctttct
      721 acacggggct gttcggctgg gagggcaccg acgtgccgac gccgatgggc ccggtgtaca
      781 ccatgttccg caaggacgcc aaactcgtcg ccggcatggg accgcagccg cccggcatgg
      841 ccgacgctgg ggtcccgtcg gtgtggaact cctacctcat ggcccctgac gtcgacgcca
      901 cggtggctgc ggttgaggtg gccggtggca cggtcgtcat gccggcgatg gacatcatga
      961 cccagggccg catggcgatg gtcgccgacc cgagcggcgc cgtcgtcggg ctctggcagc
     1021 cgcgtgacca cgagggcgcc gagctgttca acgtgcccgg ctcgctcacg tggaacgagc
     1081 tgcagacccg ggaccttgct gcggcgacgg ccttctacgc ggaggtcttc ggctggcgct
     1141 gggagccgat ggagggcagc gactatctcg tggccaacat cgatgccaag gagggggagg
     1201 acaagagcaa cggcggcgcg atgacgatgc ccgacatggt gcccgccgag gcaccgagca
     1261 tgtggaacgt ctacttcgcg gtgaccgact gcgacgagtc ggtcacccgg gcggtcgagc
     1321 tcggcgggag cgtcttcctg ccggccatga cgatgggccc cgggcgcttc gccgggatca
     1381 ccgacccgac cggtgcgatg ttcttcctcg gagcgttccc gacggcctga gccggcgagg
     1441 gggcggacct gtcggcgacg acccggcggc ccgccacctc gggcgcgtcc cagctgtggc
     1501 ggcccgaggc ggttctggtc acccaaatgt gagagtgttg tctcgatatg tctctctcac
     1561 gaaacggggc gcagcacgcc atgaccaacc cgtacggcga cgtcgagctc ggtacgcagg
     1621 ggatgcgggc gctggcgcac ccggtgcgcc tggccatcct catccagctc cagaaccatg
     1681 gcccgaaaac ggcgactgcg ctggccccgg cagtcggggc cagcccgtcc gtgacgagct
     1741 ggcacctgcg gcatctggcc aaacacgggc tcgtccggga ctccgacgtc ggcaccagcg
     1801 gccgggagcg gtggtgggag gccgcgtcgc gcggcttccg gttcacggcc gaggacgagg
     1861 ccgggcgcga ggcgcagcgg ctgctctccg ccgtgctcga ggaggtcgag ggtgacctgc
     1921 ccgggcagtg gacggccgac gtggagccgc ggctggaacc gcagtggcga agtgccgccg
     1981 gtcggtcgaa cacccgcgtt ctcgtcaccc tcgaggagct agccgagctc gagtcggcca
     2041 tcgaggcgct cctcgcgcca tacgtcctgc gcaaggacgc cggcaccgac acgatcccgg
     2101 cgcaggcccg gggcgtccgc ctccttcgct acgtcctgcc ggagctgccg gagccgacag
     2161 gggacgcgtc gtcgtgaggg acccggacgc acggcgcggt atgccgtcca cggcgccact
     2221 gtggcgcgac cggcggttct cgacgtactg gggcggtcag accgtgtccc agctcgggga
     2281 ccgggtctca gagctcgccc tgccgctcat cgcggtgacc atgctccacg cgagcccgac
     2341 ggccgtagga ctgctcacgg ccgcggtctg ggcgccgaat ctcctgtcgc tgttcgtcgg
     2401 cgcgtgggtg gaccgccagc aacgcaagcg gcggctgctc atctggggcg acctcctcca
     2461 gtgcgtcgtc atcttcagcc ttccggtcgc gtactacctc ggcaccatca ccttcgttca
     2521 cctcgttgcc gtggcgctcc tcgcggcgcg ggggccgtcc tctatcacac ggcataccca
     2581 gcgttcttcg tctccctcgt tcgacgagac caatatctcg aggcgaactc ccttctcagc
     2641 gggacgcgtt cggtctcgtt catcgtcggc ccggccctcg ccggtggcct cgttcaagcg
     2701 ctcaccgcac cggtcgccgt catcgtcgac gggctgtcct tcctcgtctc agccctgatg
     2761 a
//
LOCUS       DATAIX010000651         1530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_291112, whole genome shotgun sequence.
ACCESSION   DATAIX010000651 DATAIX010000000
VERSION     DATAIX010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291112"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VHM65_09605"
     CDS             complement(<1..774)
                     /locus_tag="VHM65_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706002.1"
                     /translation="MSGSPASWMGRLSLRARLMLVGLAGVATGLLASGLVLYGVLGAS
                     LDRSIEDSARRSAAEIATLVDEQRLPNPLPVSGAQLVQVVDASSRVVAASLTADRLTS
                     LLGGAELSAALAGRALDVPGNRAGLTGTLRAVAVGAGPSSARMTVIAAVPTADLAASQ
                     QRLRAGLLVALPLLLALMAVIAWRVIGAALRPVEALRAGAERIGGGSEGSVGSGGSVG
                     PVRSGSRSAGSVPPSPRRWATSSWVRMLARGLRSSCAASE"
     gene            complement(771..1424)
                     /locus_tag="VHM65_09610"
     CDS             complement(771..1424)
                     /locus_tag="VHM65_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2706003.1"
                     /translation="MRLLVVEDEPGMARALRRGLQAEGFVVELAWDGVAGLELARHGE
                     FDAVLLDVMLPRMSGYTVVRTLREEGNWVPVLMVSAKDGEYDQADGLDYGADDYLTKP
                     FSFVVLLARLRALLRRDPGPRPVVLEAAGVSIDPATREVTVDGIPVALSGREALVLEH
                     LVRAHPRPVTKERLLDDIWGTAEVDANNVEVYVGYLRRKLGRERIATVRGVGYRLAP"
BASE COUNT          229 a          585 c          549 g          167 t
ORIGIN      
        1 ctctgacgcc gcgcacgagc tgcgcagtcc cctcgccagc atccgcaccc agctcgacgt
       61 ggcccagcgc ctcggcgacg ggggaacgct gcctgccgac ctgctgcccg agcggactgg
      121 tccgactgag ccgcccgagc cgactgatcc ctccgatccg cccccgatgc gctcggcccc
      181 ggcgcgcagg gcctccaccg gccgcagcgc ggcaccgatg acccgccagg cgatgacggc
      241 catgagagcg agcagcaggg gcagggcgac gagcagcccc gcacgcagcc gctgctggct
      301 cgccgcgagg tcggccgtag ggacggcggc gatgaccgtc atccgggccg acgaaggccc
      361 ggcgccgacc gcgaccgccc gcagcgtccc ggtgagtccg gcccggttgc cggggacgtc
      421 gagcgcacgt ccggccagcg ccgcggagag ctcggcaccg ccgagcagcg aggtcagccg
      481 atctgcggtg agggacgccg cgacgactcg cgacgaggca tcgacgacct ggacgagctg
      541 ggctcccgac acgggcagcg ggttgggcag ccgctgctcg tcgacgagcg tggcgatctc
      601 ggcggcgctg cggcgggcgc tgtcctcgat gctgcggtcc agactggcac cgagcacgcc
      661 atacaggacc agcccgctcg cgagcagtcc ggtggcaacc ccggccagcc cgacgagcat
      721 gagccgggcc cgcaggctga gccgccccat ccacgacgcc ggggaaccgc tcacggggcc
      781 agccggtagc ccacgccgcg gaccgtcgcg atccgctcgc ggccgagctt gcgccgcagg
      841 tagcccacgt agacctcgac gttgttggcg tcgacctcgg ccgtgcccca gatgtcgtcg
      901 aggagacgct ccttggtgac cggccggggg tgtgcccgga cgagatgctc gaggacgagc
      961 gcctcccgcc cggacaacgc gaccggtatg ccgtcgaccg tcacctcacg ggtcgcgggg
     1021 tcgatgctga cgcccgcggc ctcgagcacg acgggacggg gcccgggatc gcggcgcagc
     1081 aaggcgcgca gccgggccag cagcacgacg aacgagaacg gcttggtcag gtagtcgtcg
     1141 gcgccgtagt cgagcccgtc ggcctggtcg tactccccgt ccttggccga gaccatgagc
     1201 acgggcaccc agttgccctc ctcacgcagc gtccgcacga cggtgtagcc ggacatcctc
     1261 ggcagcatca cgtccagcag cacggcgtcg aactccccgt ggcgggccag ctccagaccg
     1321 gccacgccgt cccaggcgag ctcgacgacg aagccctcgg cctgcagccc gcggcgcagg
     1381 gcacgcgcca taccgggctc gtcctcgacc acgagcagcc tcacccggcc accctgtcac
     1441 gcccggcggc gcggcgacga gtggcaggcc agcggcccgg caagggtcgt tctcagggcg
     1501 ctctcaggcc gcgggggtga agatgggtca
//
LOCUS       DATAIX010000652         4496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_291142, whole genome shotgun sequence.
ACCESSION   DATAIX010000652 DATAIX010000000
VERSION     DATAIX010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291142"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1233)
                     /locus_tag="VHM65_09615"
     CDS             complement(64..1233)
                     /locus_tag="VHM65_09615"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX2706004.1"
                     /translation="MSRPRALLTARMAGFGTTIFARMSALATSTGAINLGQGFPDTDG
                     PAQVLEAAREAIATGRNQYPPGPGVPELLEAIAAHQARFYGIRLDPRSEVLVTVGATE
                     AIAATILALCEPGDEVVTFEPYYDSYAATIALAGAARRTSVLRFPDFAVDEASLRAAF
                     SARTRLVLLNSPHNPTGKVFTRAELELVARLAVEHDAWVVTDEVYEHLVFDGREHVPV
                     ATLPGMAERTLTISSAGKTFSVTGWKVGWVSGPAAAVTAVRTVKQFLTYVGSGPFQPA
                     VALALGLGDGVYDGFAQSLQTKRDLLCDGLSAAGLTVSRPAGTYFVIADAAPLGAVDG
                     LAFCLEMPQRFGVVAVPVAVFHDDAEAARTLVRFAFCKRDEVLRDAVRRLAGSSN"
     gene            complement(1248..3185)
                     /locus_tag="VHM65_09620"
     CDS             complement(1248..3185)
                     /locus_tag="VHM65_09620"
                     /inference="COORDINATES: protein motif:HMM:NF042625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6541 family protein"
                     /protein_id="HEX2706005.1"
                     /translation="MTWAQSAPTLLVLAVAVLAPGAAMGAALGLRGPVLFGLAPSLSM
                     TALAGGAVLAPMLGLAWTPVTALAATAAAVAASVVLAAGVRRAAGPASPAPGIAVRGG
                     SHRTTDRFALAAALAAGVVIGVAAAVAMRVPDELVDSPDAVFHVNRLRVYLDGGNASS
                     LLFGYPSGFHDLAAIAIQVTHPPIIAAVNLTSLAVAAIAWPLGVLALASRVLGSSRFV
                     LAAAALASTSLTAMPYLLMGWGVLWPNVLGAAVLPGAVAALALARRGDPSQRRAAALG
                     LVVAIPGLALAHPNTLAGLLVFGGTWLVTALARRAWSAHGRTRGAALAQLGVVVGGGL
                     AALVLLPRLSPLLAATATYVWRTDPLNLSVRDILLGSLQLGPRIAVTVVAALGVGYVV
                     ARRRSHLWVVVAMGVCVLLYVLAASTTGPVTSALTGFWYNDKVRLAVLAAVPLTVLVT
                     AGLVGVRGWLVQVLASRTRRPVSVATARGAAVVVLATFVLAGGSPHDGARLLERYYWP
                     DVVDHTIASAQDRADLVRLAELVPSTAIVAGDPSNGSALLYALTGRTVLTPGLGPSAD
                     PDVVLIGQHLKELATRADVCPAVRRTGARYAVDAAPQYWGRNEAATVGIVGLIGVTGL
                     TEVGRAGRYTLFEITGCAGPS"
     gene            3509..>4496
                     /gene="rpsB"
                     /locus_tag="VHM65_09625"
     CDS             3509..>4496
                     /gene="rpsB"
                     /locus_tag="VHM65_09625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX2706006.1"
                     /translation="MAVVTMRQLLESGVHFGHQTRRWNPKMKRFIMTERNGIYIIDLQ
                     QSLTYINDAYEFVKETVAHGGSILFVGTKKQAQEPVADQATRVGMPYVNHRWLGGMLT
                     NFNTVSKRLGRLKELEEIDYDDVAGSGRTKKELLVLKREKDKLEKTLGGIRDMAKVPS
                     AVWIVDTKKEHLAVNEARKLGLPVIAILDTNCDPDEVDYKIPGNDDAIRSVSLLTRVI
                     ADAVADGLMTRAARRGGTEDVVAADEPLAEWERELLETSATEASAVSADAQGAVEAAA
                     VEPVAEPVVAEPVAEDEPVVAEPVAEDEPVAEDEPVVAEPVAEVAPVAEALAE"
BASE COUNT          732 a         1585 c         1653 g          526 t
ORIGIN      
        1 caacgggacg cgcgcgcggg aagtcgcccc cggggagggt ttcagtgcga acgggcggcc
       61 ggcctagttc gacgacccgg caagacggcg gaccgcatcg cgcagcacct cgtcgcgctt
      121 gcagaacgcg aagcgcacca gcgtccgggc cgcctcggca tcgtcgtgga agaccgcaac
      181 cggcacggcg accacgccga agcgctgcgg catctcgagg cagaaggcca gcccgtcgac
      241 cgctccgagc ggggcagcat cggcgatgac gaagtacgtc cccgcgggcc gggacacggt
      301 gagcccggcc gcactcaggc cgtcgcagag caggtcccgc ttggtctgca gagactgggc
      361 gaacccgtcg tacacgccgt cgcccagccc gagagccagc gccacggccg gctggaaggg
      421 gccggacccg acatacgtga ggaactgctt gaccgttcgc acggcggtga ccgcagcggc
      481 cgggccgctg acccagccga ccttccaccc cgtgaccgag aacgtcttgc cggccgagga
      541 gatggtcagc gtccgctcgg ccatgccggg cagggtcgcc actgggacgt gctctcgccc
      601 gtcgaagacc aggtgctcgt agacctcgtc ggtgacgacc caggcgtcgt gctcgaccgc
      661 gagccgcgcc acgagctcga gctcggcccg ggtgaagacc ttgcccgtcg ggttgtgtgg
      721 cgagttgagc aggacgagcc gcgtccgggc cgagaacgcg gcccgcagcg aggcctcgtc
      781 gacggcgaag tccgggaagc gcagcacgct cgtgcggcgg gctgcccccg cgagggcgat
      841 cgtcgcggca tacgagtcgt agtagggctc gaaagtgacg acctcgtccc cgggctcgca
      901 cagcgccagg atcgtcgcgg caatcgcctc ggtcgccccc acggtgacga gcacctcgga
      961 gcggggatcg agccggatgc cgtagaacct cgcctggtgc gcggcgatgg cctcgagcag
     1021 ctcggggacc ccgggcccgg gcgggtactg gttgcgtccg gtggcgatgg cctcccgggc
     1081 tgcctccagc acctgcgccg gtccgtcggt gtcggggaag ccctggccga ggttgatcgc
     1141 cccggtcgag gtcgccaggg cggacatccg ggcgaagatc gtcgtcccga accccgccat
     1201 ccgcgccgtc aggagcgcac gtggcctgct catggaggtc tcctcactca tgacggaccg
     1261 gcgcagccgg tgatttcgaa gagggtgtac cgaccggccc ggccgacctc ggtcaggccg
     1321 gtgaccccga tgagaccgac gataccgacc gtcgcggcct cgttgcggcc ccagtactgc
     1381 ggtgcggcat cgacggcata gcgggcaccg gtccgtcgca ccgcggggca gacgtccgcc
     1441 cgcgtcgcca gctccttgag gtgctgaccg atgagcacga cgtcagggtc cgcggacggg
     1501 ccgaggccgg gggtgaggac ggtgcgcccg gtgagggcgt agagcagggc cgagccgttc
     1561 gaggggtccc cggccacgat ggccgtcgag gggaccagct cggccagccg cacgaggtcg
     1621 gcgcggtcct gcgcgctcgc gatggtgtga tcgacgacgt ccggccagta gtagcgctcg
     1681 agcaggcggg caccgtcgtg gggtgacccg ccggccagca cgaacgtcgc gagcaccacg
     1741 acagcagcgc cccgcgcggt cgccaccgag acaggacggc gggtccgcga ggcgagcacc
     1801 tgcacgagcc acccccggac acccacgagc cccgcggtga cgagcacggt gagcggaaca
     1861 gcggcgagga ccgccaggcg caccttgtcg ttgtaccaga acccggtcag ggccgaggtc
     1921 accggcccgg tcgtcgacgc cgcaaggacg tacagcagga cacagacacc cattgccacg
     1981 acgacccaca ggtgtgaccg tcggcgcgcg acgacatagc cgacccccag tgccgccacg
     2041 accgtgacgg cgatgcgggg accgagctgg agcgagccga gcaggatgtc gcgcaccgac
     2101 aggttcagcg ggtcggtccg ccagacgtag gtcgctgtcg cggcgagcag cggggagagc
     2161 ctgggcagca gtacgagcgc ggcgagcccg ccgccgacca cgacgccgag ctgagcaagc
     2221 gcagcccccc gcgtccgccc atgcgcggac caggcacggc gcgccagcgc agtgaccagc
     2281 caggtccccc cgaagaccag caaccccgcg agggtgttgg ggtgagccag agccaggccc
     2341 ggtatggcga cgaccaggcc gagcgcggcc gctcgccgct ggctcgggtc gcctcgccgg
     2401 gccagggcga gtgcagccac ggccccggga aggacggcgg caccgaggac gttgggccag
     2461 agcacccccc agcccatgag gagatacggc atcgcggtga gcgaggtgga cgccagggcc
     2521 gcggcggcga ggacgaagcg tgagctgccg agcacccggc tggccagggc gagaacgccg
     2581 aggggccagg cgatcgcggc gaccgcgagg ctggtgaggt tgacggcggc gatgatcggc
     2641 gggtgggtga cctggatcgc gatcgcggcc aggtcgtgga agccgctggg gtagccgaaa
     2701 agcaacgacg aggcgtttcc cccgtcgagg tagacccgca gccggttgac atgaaagacc
     2761 gcgtccggac tgtcgacgag ctcgtccggg acgcgcatgg ccaccgcggc ggccactccg
     2821 atgacgacgc ctgccgccag cgccgccgcg agggcgaacc ggtcggtagt gcggtgactc
     2881 ccgcccctga cggcgatgcc gggcgcgggt gaggccggac ccgcggcccg gcgcactccg
     2941 gccgccagga ctaccgaggc ggccacggcc gctgcggtgg cggcgagcgc cgtcacggga
     3001 gtccacgcca gcccgagcat cggggcgagc accgcgcccc cggcgagtgc cgtcatactc
     3061 aggcttggcg cgaggccgaa gaggaccggc ccgcgcaggc cgagcgcggc gcccatcgcg
     3121 gcacccggag cgagcaccgc gacggcgagc acgagcagcg ttggggctga ctgcgcccac
     3181 gtcatcggcg gtcctcgtct ggcaggtgct tgcgcagggg agtgacctgc gcctcggcga
     3241 cgagcgtagt ggccgggcct gggtgccggc gcctcccggt gcgggggtcg cgatttccgg
     3301 atcgtccctg cgtgtccgta gaatcggtcc cacgccggtc ccctttcgtc ggggtcggcg
     3361 aattcgcgtg ccttctcacc gccgtatggc ggggcgcacc acagcagtcc cggtcaccga
     3421 cccgggcggg gtgcggacgc aggcaccagg cacggtgcgg cgcccgccgc gtccgtggca
     3481 actgacaacg aggaagaagg accacggcat ggccgtcgtc acgatgcgcc agctccttga
     3541 gagcggcgtc cacttcgggc accagacccg tcgctggaac cccaagatga agcgcttcat
     3601 catgaccgag cgcaatggca tctacatcat cgacctgcag cagtcgctca cctacatcaa
     3661 cgacgcctac gagttcgtca aggagaccgt cgcccacggc ggctcgatcc tgttcgtcgg
     3721 cacgaagaag caggcgcagg agcccgtcgc cgatcaggcg acccgggtcg ggatgcccta
     3781 cgtcaaccac cgctggctcg gcgggatgct cacgaacttc aacacggtct ccaagcggct
     3841 cggccgcctc aaggagctcg aggagatcga ctacgacgac gtggcgggct ccggccgcac
     3901 gaagaaggag ctcctcgtcc tcaagcgcga gaaggacaag ctcgagaaga ccctcggcgg
     3961 catccgtgac atggccaagg tcccctcggc ggtgtggatc gtcgacacca agaaggagca
     4021 cctcgcggtc aacgaggccc gcaagctcgg cctgccggtc atcgcgatcc tcgacaccaa
     4081 ctgcgacccg gatgaggtcg actacaagat cccgggcaac gacgacgcga tccgttcggt
     4141 ctccctgctg acccgggtca ttgctgatgc cgtcgccgac ggcctcatga cccgcgcggc
     4201 ccgccgcggc ggcaccgagg acgtcgtcgc ggccgacgag ccgctggccg agtgggagcg
     4261 cgagctgctg gagacctcgg caaccgaggc ttccgcggtg tcggctgacg cgcagggtgc
     4321 ggtcgaggcg gctgctgtcg agcccgttgc cgagcccgtc gtcgccgagc ccgtcgccga
     4381 ggacgagccc gtcgtagccg agcccgtcgc cgaggacgag cccgtcgccg aggacgagcc
     4441 cgtcgtagcc gagcccgtcg ccgaggttgc gcccgtcgcg gaagccctcg ctgagg
//
LOCUS       DATAIX010000653         1615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_291243, whole genome shotgun sequence.
ACCESSION   DATAIX010000653 DATAIX010000000
VERSION     DATAIX010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291243"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VHM65_09630"
     CDS             <1..916
                     /locus_tag="VHM65_09630"
                     /inference="COORDINATES: protein motif:HMM:NF025064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706007.1"
                     /translation="LKAHLDGLGDSVLVVGSGDLWNVHAHVDDVGAAIEAGIEAGRPR
                     RITVTHFGDQVGDQVGDQVGDQGRGRAEASCAEPTAMAPRAALGVVACAAGEGLAVAF
                     ESVGAVVVRGGPGRRASAGQLVEACRMAHAHRILVLPNDPDMVLAAQAAASAAAKEGM
                     TVTVLPSVAAVQGVAALAVYDPGGDPDETAEAMAAAVIATRHGSVSVATRTSDTAVGP
                     CREGDVLGLVNGHVTVIGDDLIEVATAVVTELLGAETELVTIVSGADAPTGLAQRVQD
                     AVAAMAPEVEVDHVDGGQGVHSLLFGVE"
     gene            917..>1615
                     /locus_tag="VHM65_09635"
     CDS             917..>1615
                     /locus_tag="VHM65_09635"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706008.1"
                     /translation="MPPGRRAATAPPGPGTLTEASGLDKQFGTAVVSLLEVNRGITTV
                     GQLLDYLPRRYLEPGALTDLAGLVPGEEVVIVARIASVTSRAMKYRKGDVVTVTVTDG
                     VSEVELTFFSKKATYPLIPGRRAFFAGRVEEFNRSVTLTHPTFEVFKSDDMSALDDYR
                     EHLIPIYSASGALQSWKVARAVQWALGSLGPLPDFLPEQIRERHGLLARDAAMRAVHR
                     PRNHAEARAARARLR"
BASE COUNT          217 a          589 c          587 g          222 t
ORIGIN      
        1 gctcaaggct catctcgacg ggctcggcga ctcggtgctc gtcgtcggct ccggtgacct
       61 gtggaacgtc cacgcccacg tcgacgacgt cggcgccgcg atcgaggccg ggatcgaggc
      121 cgggcgcccc cgccggatca cagtgaccca cttcggcgac caggtgggcg accaggtggg
      181 cgaccaggtg ggcgaccagg ggcgcggccg ggccgaggcg tcgtgcgcgg agccgacggc
      241 catggccccc cgggccgcgc tcggggtcgt agcctgcgcc gcaggtgagg ggctggcggt
      301 cgccttcgag tcggtgggag cggttgtcgt ccgtggcgga cccggtcggc gcgcctcggc
      361 cgggcagctg gtcgaggcct gcaggatggc ccacgcgcac cgcatcctgg tgctgcccaa
      421 cgacccggac atggtcctcg cggcccaagc cgcggccagc gcggccgcga aggagggcat
      481 gacggtgacg gtgctgccgt ccgtagcggc cgtgcagggg gtcgcggcac tcgcggtcta
      541 cgaccccggc ggcgatcccg acgagacggc cgaggccatg gcggcggccg tcatcgccac
      601 ccgtcacggc tcggtttccg tcgccaccag gacgtccgac accgccgtcg gcccctgccg
      661 cgagggggac gtcctcggcc tcgtgaacgg tcacgtgacg gtcatcggcg acgacctcat
      721 cgaggtcgcc acggccgtcg tgaccgagct gctcggagcc gagaccgagc tcgtgacgat
      781 cgtgtccggc gcggatgccc cgaccggtct ggcgcagcgg gtgcaggacg cggtcgcggc
      841 catggccccc gaggtcgagg tggaccacgt cgacggcggg cagggggtcc actccctgtt
      901 gttcggggtc gagtagatgc cgcccggccg tcgcgcggcc acggccccgc ccggtcctgg
      961 cacgctcacc gaggcctccg gtctcgacaa gcagttcggg accgccgtgg tctcactcct
     1021 cgaggtcaac cggggcatca ctacagtcgg ccagctgctc gactacttgc cccgccgcta
     1081 tctcgagccc ggagccctca ccgacctcgc cggcctcgtg cccggcgagg aggtcgtcat
     1141 cgtcgcgcgg atcgcctcgg tgacgagccg tgcgatgaag taccgcaagg gcgatgtcgt
     1201 caccgtgacg gtcaccgacg gcgtgagcga ggtcgagctc accttcttct ccaagaaggc
     1261 gacctacccc ctcatcccgg gtcgccgcgc gttcttcgcg ggcagggtcg aggagttcaa
     1321 caggtcggtc acgctgacgc acccgacctt cgaggtcttc aagtccgacg acatgagcgc
     1381 cctggatgac taccgcgagc acctgatccc gatctacagc gcgagcggcg ccctgcagtc
     1441 gtggaaggtc gcccgcgccg tccagtgggc gctcggctcg ctcggcccgc tgccggactt
     1501 cctgcccgag cagatccgtg agcgccacgg gctgctcgcg cgcgacgccg cgatgcgggc
     1561 cgtgcaccga ccccgcaacc acgcagaagc gagggccgcc cgggcgcggc tgagg
//
LOCUS       DATAIX010000654         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_231083, whole genome shotgun sequence.
ACCESSION   DATAIX010000654 DATAIX010000000
VERSION     DATAIX010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231083"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VHM65_09640"
     CDS             complement(<1..278)
                     /locus_tag="VHM65_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706009.1"
                     /translation="MSSALAPPRGSEMQEAPAAATHRPARPTVRRSWWPDVIGSLTVL
                     SLVVVAALWILGGGLTGVDSFATAMTSLGRITGLLASDLLLIQVLLMAR"
     gene            complement(362..1873)
                     /locus_tag="VHM65_09645"
     CDS             complement(362..1873)
                     /locus_tag="VHM65_09645"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX2706010.1"
                     /translation="MSDLLIGIDMGTGSSKGVLATSDGTIIATATRPRPRSMSMPRPG
                     WAEADAEGVWWTDVRSISRELMGQAVAGTVRGVCVSGVGPCLVLADADDVPVRPAILY
                     GIDMRATEEIDELTERYGAQEILARGGTPLTTQAVGPKALWVQRREPEVWARARAWYN
                     SSSYVVKRLTQEYVLDHHTASQCDPLYDIRANAWYEPWVRDLMGTLPMPRLVWPSEVA
                     GVVCAEAAAATGIPEGTPVCAGTVDAWAEAFSAGVRAPGDLMLMYGSTMFFVQVLHGL
                     ASHPQLWTTAGVDRGTYTLAAGMATSGSLTSWVQDLVGGVPFDQLVREAAEVPAGSDG
                     LLLLPYFAGERTPIFDARARGVIAGLSLRHTRAHLFRAVYEGIAFGIRQILELLDGDI
                     STVTRLVAVGGGTQGGLWTQIVSDVTGREQEVPVHTVGASYGDALMAAIGAGLVPPSA
                     NWAVIGRRVRPNPAARAVYDQMYASYCELYPATRPFVHQLARMQEEHADPASA"
BASE COUNT          325 a          798 c          765 g          299 t
ORIGIN      
        1 cgggccatga gcagcacctg gatgagcagc aggtcgctgg cgagaaggcc ggtgatgcgc
       61 ccgaggctgg tcatggcggt cgcgaacgag tcgacgcccg tcagcccacc cccgaggatc
      121 cacagggccg cgaccacgac cagcgacagc accgtgaggc tcccgatgac gtccggccac
      181 cacgaccggc ggaccgtcgg gcgcgcgggc cggtgggtcg ctgccgccgg cgcttcctgc
      241 atctcggacc cccgtggtgg tgccagcgct gagctcatac cccgaagggt tcccggcctc
      301 cctgtgcgcg acctgtgcgc ttccctgagc ccaggctgtg agtcgctcgc cgcgctcgtg
      361 ctcaggcgga cgccgggtcg gcgtgctcct cctgcatgcg cgccagctgg tggacgaagg
      421 gccgggtggc cgggtagagc tcgcagtagc tcgcgtacat ctggtcgtag acggcccgcg
      481 cggcggggtt cggcctcacc cgccggccga tgacggccca gttcgccgag gggggaacga
      541 gcccggcgcc gatggccgcc atgagtgcgt caccgtatga cgcgcccacc gtatgcacgg
      601 ggacttcctg ctcgcggccg gtcacgtcgc tgacgatctg ggtccacaac ccaccttggg
      661 tgccgccgcc gacggcgacg aggcgggtca ccgtgctgat gtccccgtcg agcagctcga
      721 gaatctggcg gatcccgaac gcgatccctt cgtagaccgc gcggaacagg tgagccctgg
      781 tatggcgcag gctcagaccc gcgatgacgc cccgggcgcg cgcgtcgaag atcggggtcc
      841 gctcccccgc gaagtacggc agcagcagga gcccgtccga gcccgccggc acctcggccg
      901 cctcccggac gagctggtcg aacggcaccc ctccgaccag gtcctgaacc cacgaggtca
      961 ggctgcccga cgtcgccatc ccggccgcca gcgtgtaggt cccgcggtcg acgccggccg
     1021 tggtccacag ctgcgggtgc gaggcgaggc catggaggac ctggacgaag aacatggtcg
     1081 agccgtacat cagcatcagg tcccccggag cccggacgcc ggcgctgaag gcctcggccc
     1141 acgcgtccac ggtcccggcg cacacgggcg tcccctcggg tatgccggtc gcggcggcgg
     1201 cctcggcgca gacgaccccg gcgacctcgc tcggccacac gagccggggc atcgggaggg
     1261 tgcccatgag gtcgcgcacc cacggctcgt accaggcgtt ggcccggatg tcgtagagcg
     1321 ggtcgcactg gctggcagtg tggtggtcga ggacgtactc ctgggtgagc cgcttgacga
     1381 catacgagct cgagttgtac caggctctgg ccctggccca gacctcgggc tcgcgccgct
     1441 ggacccagag tgccttgggc ccgacggcct gggtcgtgag cggggtcccg ccgcgggcga
     1501 ggatctcctg cgcgccatac cgctcggtga gctcgtcgat ctcctcggtg gcgcgcatgt
     1561 cgatgccata gagaatcgcc gggcggacgg gcacgtcgtc ggcgtcggcc aggacgaggc
     1621 acggaccgac tccgctgacg cacaccccgc ggaccgtgcc ggcgaccgcc tggcccatca
     1681 gctcccggga gatcgacctg acgtcggtcc accaaacgcc ctcggcgtcg gcttcggccc
     1741 acccggggcg gggcatcgac atcgagcgcg gccgcggccg ggtcgccgtc gcgatgatcg
     1801 tgccgtcgga ggtcgccagc acccccttgc tgctcccggt ccccatgtcg attccgatca
     1861 gcagatcgct cattgttgtc catccagcac cgacggtgac catggcggga gcagctcctc
     1921 cgggagggac accatcacct tgggcatacc gcaacggtag ccgtgggtgt tgctctgacc
     1981 ggtccgcctg ccttcctgct catcctcacc acggtcggtc accgggacac cagcgcaggc
     2041 aacgcgcccc tgctgggcgg cgcacccccg acggagggcg gggagacgct cccacctcag
     2101 cgcatcgagg aggccatgga ccacccgatc cggatggcaa acgcaacgat gacgacgagc
     2161 agcaccacac ggacgaagcc gttgccg
//
LOCUS       DATAIX010000655         1891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_256764, whole genome shotgun sequence.
ACCESSION   DATAIX010000655 DATAIX010000000
VERSION     DATAIX010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256764"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1865)
                     /gene="cutA"
                     /locus_tag="VHM65_09650"
     CDS             complement(<1..1865)
                     /gene="cutA"
                     /locus_tag="VHM65_09650"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF040766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HEX2706011.1"
                     /translation="MTGFALAPDHVNHVGEPIVMVVAADRYLAEDACERVKIAYEQLP
                     VVVGVEAARAAGHLVHDEAPGNVAAHLVQEVGDVRAAIDAAPHRLTLDLDIERSASMP
                     MEGRGVYARWDGDEGSLRIYSSTQTTTGVRAAVAARLDLPLAKVECIAPDVGGGFGVK
                     IMHPWPEEVLVPWAARRLGREVKWTEDRREHFISSSHERAQIHRVEVGFDGAGRILGL
                     DVTFWHDNGAYTPYGIIVPIITSTQLLGPYKPGTYRCEFFSLYTNTVIVTPYRGAGRP
                     QGVFAMERTMDAIADTLGLDRADVRSANFILPEEMPYDHGLLFQDGRPLRYDSGDFPA
                     SLAKLKALVGWDGFAPYREQAEREGRRVGLGIGCYVEGTGVGPYEGGHVQIETSGRVN
                     VATGLTTQGQGHQTSFAQIVADELGVRIEDVYVTTGDTRRMPYAVGTFASRAAVMSGN
                     AIALAARNVREKALRLAGDALEVDPRDLEIVDGIVRVKGSPETSVALGTLSVLSNPLR
                     YAFDDAAQAATQFAGTADLTTAPVADDEEPGLEGRDYYSPTQATFANGMHAAIVETDP
                     LTAEITILRYCVIHDCGTMINPMIVEGQVHGGVAQGVGGALYERMVYDEAGQLLNA"
BASE COUNT          256 a          647 c          694 g          294 t
ORIGIN      
        1 gcgttaagca gctgccccgc ctcgtcatac accatgcgtt cgtagagtgc cccacccacg
       61 ccctgggcaa ccccgccgtg gacctggccc tcgacgatca tcgggttgat catggtgccg
      121 cagtcatgga tgacgcagta gcgcaggatc gtgatctcgg cggtgagcgg gtcggtctcg
      181 acgatcgcgg cgtgcatgcc gttggcgaac gtcgcctggg tcggggagta gtagtcccgg
      241 ccctccaggc ccggctcctc gtcgtcggcc accggagccg tggtgaggtc ggcggtgccg
      301 gcgaactgcg tcgcggcctg ggcggcgtcg tcgaacgcat accgcagggg gttggacagc
      361 accgacagcg tcccgagcgc gaccgacgtc tcggggctgc ccttgacgcg gacgatcccg
      421 tcgacgatct cgaggtcccg ggggtcgacc tccagcgcgt ccccggcgag gcgcagcgcc
      481 ttctcgcgga cgttgcgtgc cgccagggca atcgcgttgc cactcatcac cgcggccctc
      541 gaggcgaacg tgcccacggc atacggcatc cggcgggtgt caccggtggt gacgtagaca
      601 tcctcgatcc gcaccccgag ctcgtcggcg acgatctggg cgaacgaggt ctggtgtccc
      661 tggccctggg tcgtgaggcc ggttgccacg ttgacgcgcc cgctcgtctc gatctggacg
      721 tgcccgccct catagggccc gacaccggtt ccctcgacgt agcagccgat cccgagcccg
      781 acgcggcgcc cctcgcgctc ggcctgctcg cggtatggcg cgaagccgtc ccagccgacg
      841 agcgccttga gcttggccag gctcgccggg aagtcgcccg agtcgtaccg cagcgggcgc
      901 ccgtcctgga agagcagccc gtggtcgtag ggcatctcct cggggaggat gaagttggcg
      961 ctgcgcacgt cggcccggtc cagcccgagg gtgtcggcga tcgcgtccat cgtgcgctcc
     1021 atcgcgaaca cgccctgggg ccgtcccgcg ccgcggtagg gcgtgacgat gacggtgttc
     1081 gtgtagaggc tgaagaactc gcagcggtag gtgcccggct tgtacgggcc gagcagctgg
     1141 gtcgaggtga tgatcgggac gatgatcccg tatggcgtgt aggcgccgtt gtcgtgccag
     1201 aacgtcacgt cgagcccgag gatgcgcccg gcgccgtcga agccgacctc gacccgatgg
     1261 atctgggcgc gctcgtgcga gctggagatg aagtgctcgc ggcggtcctc ggtccacttg
     1321 acctcgcggc ccagccgccg ggcggcccac gggacgagga cctcctcggg ccacgggtgc
     1381 atgatcttga cgccgaaccc gccgcccacg tcgggagcga tgcactcgac cttggccagc
     1441 ggcaggtcga gccgggccgc gaccgcggcg cgcacaccgg tcgtggtctg ggtcgaggag
     1501 tagatccgca gcgacccctc gtcgccgtcc cagcgggcgt agacgcccct gccctccatc
     1561 ggcatggacg cgctgcgctc gatgtcgagg tcgagagtca ggcggtgcgg tgcggcgtcg
     1621 atcgctgcgc ggacgtcccc gacctcctgg accagatggg ccgcgacgtt gccgggtgcc
     1681 tcgtcgtgga cgagatgccc ggcggcgcgg gccgcctcca ccccgacgac gacgggcagc
     1741 tgctcgtagg cgatcttgac ccgctcgcag gcgtcctcgg cgaggtagcg gtcggccgcg
     1801 acgaccatga cgatcggttc gccgacgtgg ttgacgtggt cgggcgcgag ggcgaagccg
     1861 gtcatcgggt gggtcaggct ggggtggggg a
//
LOCUS       DATAIX010000656         2640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_231096, whole genome shotgun sequence.
ACCESSION   DATAIX010000656 DATAIX010000000
VERSION     DATAIX010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231096"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..573)
                     /locus_tag="VHM65_09655"
     CDS             complement(118..573)
                     /locus_tag="VHM65_09655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX2706012.1"
                     /translation="MDLYQEIILDHYRRPHHAGLREPFDTEVHHINPTCGDEVTLRVH
                     VDSTSGSAVVGDLSYDAHGCSISVASTSVLADEVIGRPVIEALGTVAEMKAMLTSRGQ
                     IAGDEDVLGDGVAFAGVSKYPARVKCALLGWLAFTDALARAGVDIAAVG"
     gene            complement(574..1905)
                     /locus_tag="VHM65_09660"
     CDS             complement(574..1905)
                     /locus_tag="VHM65_09660"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HEX2706013.1"
                     /translation="MSTTTDFDLLPAGMPGSGGLGALSDAEVARLRADFPILTRTVRG
                     GRPLVYLDSGATSQKPRQVLDAEREFYERHNAAVHRGAHQLAEEATDAYESAREHIAA
                     FIGAPAREVIFTKNATEAINLAAYAFSNATAGAFVMGPGDEILVTEMEHHANLVPWQE
                     LSRRTGATLRWVPVTSGGELDLTHLDELLTPRTKVFAFTHVSNVLGTVNPVRMLADRA
                     HEVGAIVVLDACQSVPHLPIRVSELGVDLLAFSGHKMLGPLGIGVLWGRYGLLAQMPP
                     FITGGSMIELVHMEGSTYAPPPQRFEAGVPMAAQAVGLAAAADYLTALGMDRVATHDT
                     HVVAHALTALAERPWVRVVGPTDAARRVGAVSFVVDGVHAHDVGQILDDSGVEVRVGH
                     HCAWPLHRGLGVVATTRASFAPYTTLGEVDAFVAALDRVPGIFGVATAGRA"
     gene            complement(1905..>2640)
                     /gene="sufC"
                     /locus_tag="VHM65_09665"
     CDS             complement(1905..>2640)
                     /gene="sufC"
                     /locus_tag="VHM65_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX2706014.1"
                     /translation="LEVTVDVEGGVKEILRGVDLTVRGGETHAIMGPNGSGKSTLAYS
                     IAGHPKYTVTGGTVTLDGENVLAMSVDQRARAGLFLAMQYPVEVPGVTVSNFLRTAKT
                     AIDGEAPKLRTWAKEVKGAMEALKMDPVFAERNVNEGFSGGEKKRHEILQMELLRPRI
                     AILDETDSGLDIDALRVVAEGVNRIKGASEMGVLLITHYTRILRYIRPDFVHVFVDGR
                     FVEEGGPELADRLEAEGYDRFVGAPA"
BASE COUNT          402 a          899 c          950 g          389 t
ORIGIN      
        1 ggccgaggtc gacgacgttg atcccgagct cggggtcgag gacgtcacgg agggcctcct
       61 cgacgtccgc cacatcggcc gggctcccgg ttgtgatctc actcatggtc agctctccta
      121 tccgacagca gcaatatcga cgcctgcacg cgcaagagcg tcggtgaacg ccagccagcc
      181 gagcagcgca cacttgaccc gggcggggta cttcgagacc ccggcgaagg caacgccgtc
      241 accgagcacg tcctcgtcac cggcgatctg gccccgcgag gtcagcatgg ccttcatctc
      301 ggcaacggta ccgagcgctt cgatcacggg gcggccgatc acctcgtcgg cgagcaccga
      361 cgtcgaggcg accgagatcg agcagccgtg cgcgtcgtag gagaggtccc cgacgaccgc
      421 agaccccgag gtcgagtcga cgtggacccg cagggtcacc tcgtcaccac aggtggggtt
      481 gatgtggtgg acctcggtgt cgaagggctc gcgcagcccc gcgtgatgcg gccgccggta
      541 gtggtcgagg atgatctcct ggtagaggtc catctaggct cgccccgcgg tcgcgacgcc
      601 gaagatcccc gggacccggt cgagtgccgc cacgaacgcg tcgacctcac ccagagtcgt
      661 gtatggcgcg aagctcgcgc gcgtcgtcgc gacgacgccc aggccccggt gcagcggcca
      721 cgcacagtga tgtcccacgc ggacctcaac gccggagtcg tcgagaatct gcccgacgtc
      781 gtgagcgtgc acaccgtcga cgacgaatga caccgcgccg acccgccgcg ccgcatcggt
      841 cgggccgacg acgcgcaccc acgggcgctc ggcgagggcg gtgagcgcat gggcgacgac
      901 gtgggtgtcg tgggtggcga cccgatccat cccgagcgcg gtgaggtagt cggccgccgc
      961 cgcgagcccg accgcctggg ccgccatcgg cacgccggcc tcgaaccgct ggggcggcgg
     1021 ggcgtaggtc gagccctcca tgtggacgag ctcgatcatc gagccgccgg tgatgaaggg
     1081 cggcatctgg gcgagcagcc cgtagcggcc ccagaggacg ccgatgccga gcggaccgag
     1141 catcttgtgc ccgctgaacg cgagcaggtc gacgccgagc tcgctcacgc ggatcggcag
     1201 gtgcgggacc gactggcacg cgtcgagcac gacgatggca ccgacctcgt gggcccggtc
     1261 ggcgagcatc cgtaccgggt tgaccgtccc gaggacgttg ctcacgtggg tgaaggcgaa
     1321 caccttggtg cgcggggtga gcagctcgtc gaggtgggtg aggtcgagct caccgcccga
     1381 ggtgacggga acccagcgca gggtggcacc ggtgcgccgc gacagctcct gccagggaac
     1441 gaggttggcg tggtgctcca tctcggtgac gaggatctcg tcaccggggc ccatgacgaa
     1501 cgcccccgcc gtggcgttgc tgaaggcgta ggccgcgagg ttgatcgcct ctgtcgcgtt
     1561 cttggtgaag atcacctccc gggccggagc gccgatgaac gcggcgatgt gctcgcgcgc
     1621 cgactcgtag gcgtcggtgg cctcctcggc cagctggtgg gcaccgcggt ggaccgccgc
     1681 gttgtgccgt tcgtagaact cacgctcggc gtcgaggacc tggcggggct tctgagaggt
     1741 cgcgcccgag tcgaggtaga ccagggggcg cccaccccgg accgttcgcg tcaggatcgg
     1801 gaagtcggcc cgcaggcggg ccacctccgc gtcgctgagc gcgcccaatc ccccgctgcc
     1861 cggcatacca gcggggagaa ggtcgaagtc agtggtcgtg gacatcaggc cggtgcaccc
     1921 acgaaccggt cgtagccctc ggcctcgagc cggtcggcca gctccgggcc gccctcctcg
     1981 acgaagcgcc cgtcgacgaa gacgtggacg aagtcgggcc tgatgtagcg caggatccgg
     2041 gtgtagtgcg tgatgagcag gacgcccatc tcggaggcgc ccttgatccg gttgacgcct
     2101 tcggcgacga cccgcagggc gtcgatgtcc aggcccgagt cggtctcgtc gaggatcgcg
     2161 atgcggggcc gcaggagctc catctgcagg atctcgtggc gcttcttctc cccgccggag
     2221 aacccctcgt tgacgttgcg ctcggcgaag accgggtcca tcttgagcgc ctccatggca
     2281 cccttgacct ccttggccca ggtccgcagc ttgggggcct ccccgtcgat ggcggtcttg
     2341 gccgtccgca ggaagttcga gacggtgacg cccggcacct cgacggggta ctgcatggcg
     2401 aggaagagcc ccgcccgcgc gcgctggtcg acgctcatcg cgaggacgtt ctcaccgtcg
     2461 agggtgaccg tgccgccggt gacggtgtac ttggggtgcc cggcgatcga gtaggccagc
     2521 gtggacttcc ccgagccgtt gggccccatg atcgcgtggg tctcgccgcc gcggacggtg
     2581 aggtcgaccc cgcgcaggat ctccttgacg ccaccttcga cgtcgacggt gacctcgagg
//
LOCUS       DATAIX010000657         1739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_201257, whole genome shotgun sequence.
ACCESSION   DATAIX010000657 DATAIX010000000
VERSION     DATAIX010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201257"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..394)
                     /locus_tag="VHM65_09670"
     CDS             complement(200..394)
                     /locus_tag="VHM65_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706015.1"
                     /translation="MVGGALALALFGLVLPRRQRRQGALQRGPGTHHGYLFVRRVSTR
                     GFETAALVATALGGALLLVR"
     gene            617..>1739
                     /locus_tag="VHM65_09675"
     CDS             617..>1739
                     /locus_tag="VHM65_09675"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX2706016.1"
                     /translation="MSSKPRRVLLACDKFKGSLTAVDVAAALARGIAAAAPAARTVRV
                     PVADGGDGTLDAAVAAGFAPIPVVASGPTGEPVQTRYAVRDDLAVVEMADACGLLRLP
                     GGPLHPMTASSRGLGEVIAAALDAGFGRLVVGIGGSASTDGGAGMLAALGARMLDGHG
                     SDLPDGGGALGDLATLDLSALHPAVAGADIVLASDVTNPLLGTMGAPTVFAPQKGASP
                     EQVDQLEAALTRFAAVVTAATGRDDSARPGAGAAGGVGYAAQAVLGARMRPGIDVVLE
                     MSRFAELVADAELVVTGEGSLDRQTLLGKTPAGVARAATAAGVPVVAVCGRSLLAAPE
                     VHDTGIAAVYALTDLEPDPEVCMRQAGPLLERLGARLAAE"
BASE COUNT          238 a          648 c          642 g          211 t
ORIGIN      
        1 cccgatcggg ctgtgcgaca cgctcgtcgg catccggaac ggcgtgtagt acgggaagtc
       61 tggggacatc gacccgacca ccagcgcagt cagcggcagc ccgagccgac gcagcggaag
      121 gaccgcggcg gggtggctca gggtgaacgg cacaccagca cggtatgtcg gcgcgctggc
      181 aggtgtcctg caatgcgcct tatcggacga ggagcagggc gcccccgagc gcggtcgcca
      241 cgagcgcggc ggtctcgaac ccgcgagtcg agacccggcg gacgaagagg tagccgtgat
      301 gagtcccagg cccacgctga agggcacctt ggcgacgttg acgacgagga agaaccaggc
      361 cgaagagcgc aagggcaagg gcgcctccga ccacgccccg tcgtcgtcca gctccacagc
      421 tgacctcgcc gttggcgggc aggccacagc acgaggtggt gcccctttcg cgcgctatgc
      481 cctgcccgcc cctggcgggc agggcatagc gccgcgagcc gacaccccga tacccgacgc
      541 cccgaaaccg gacaccgggc cgaccacgcc cgttccgggg ctggttgaca cagcgttgct
      601 gacacacaat ggcccggtgt cgtcgaagcc gcgccgggtg ctcctggcct gtgacaagtt
      661 caagggatcg ctcaccgccg tcgacgtggc cgcggcgctg gcgcgcggga tcgctgccgc
      721 cgcgccagct gcccggaccg tgcgggtgcc cgtcgccgat gggggcgacg gcaccctcga
      781 cgcggcagtg gcggccgggt tcgccccgat ccccgtcgtc gcgagcggcc cgaccggaga
      841 gccggtccag acccggtatg ccgtccgcga cgacctcgcc gtcgtcgaga tggccgacgc
      901 gtgcggactg ctccggctgc ccggcggccc gctgcacccc atgaccgcgt cgagccgcgg
      961 gctcggtgag gtcatcgccg cggcactcga tgccgggttc ggccgcctcg tcgtcgggat
     1021 tggcggaagc gccagcacgg acggcggcgc cgggatgctc gctgcgctcg gggcacggat
     1081 gctcgatggc cacgggtcgg acctgcccga cggcggcggg gcgctgggcg acctcgccac
     1141 cctcgacctg tccgccctgc acccggccgt ggccggggcc gacatcgtcc tggccagcga
     1201 cgtgaccaac cccctgctgg gcaccatggg cgcgccgacg gtcttcgcgc cccagaaggg
     1261 agcctcaccc gagcaggtcg accagctcga ggcagccctg acgcggttcg cggcggtcgt
     1321 caccgccgcg accgggcgcg acgacagcgc gcggccggga gccggagccg ccgggggcgt
     1381 cgggtatgcc gcgcaggcag tcctgggtgc ccggatgcga cccggcatcg acgtcgtcct
     1441 ggagatgtcg cgcttcgccg agctcgtcgc cgacgcggag ctcgtcgtga cgggcgaagg
     1501 gtccctcgac cggcagaccc tgctcggcaa gacgcccgcc ggggtcgccc gggcggccac
     1561 ggcggcgggg gttcccgtcg tcgcggtgtg cgggcgctcg ctcctggccg cgcccgaggt
     1621 ccacgacaca ggcatcgccg cggtctacgc gctcaccgac ctcgagcccg accctgaggt
     1681 ctgcatgcgc caggcgggcc ccctcctgga acgcctcggt gcccggctcg ccgctgagt
//
LOCUS       DATAIX010000658         1866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_201269, whole genome shotgun sequence.
ACCESSION   DATAIX010000658 DATAIX010000000
VERSION     DATAIX010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201269"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..851)
                     /gene="prcA"
                     /locus_tag="VHM65_09680"
     CDS             complement(69..851)
                     /gene="prcA"
                     /locus_tag="VHM65_09680"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HEX2706017.1"
                     /translation="MPFYVSPEQLMKDRADYARKGIARGRMVVAMGFDSGIAFVAENP
                     SRTLHKISEIYDRIAFAAVGKYNEFENLRVAGVRYADLRGYSYDRSDVTARGLANAYA
                     QTLGAVFTQESKPYEVEIVVAEVGHSPDQDQIYRLTYDGSVADERGFVAMGGASEAAE
                     KRLAEAWEPGLTMADVLDLAVEVLAADPAGGAPRPLGADALEVAVLDRNRPRRAFRRI
                     LGPLLEDLLSADSPTTDVPSVDDPNPGHHDVLSASGDGSGAP"
     gene            complement(854..1681)
                     /gene="prcB"
                     /locus_tag="VHM65_09685"
     CDS             complement(854..1681)
                     /gene="prcB"
                     /locus_tag="VHM65_09685"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HEX2706018.1"
                     /translation="MTTGGFAGSLPAPYRQVGSSSFTDFVAAQAPSLLPAGRPLPAGV
                     VPEAPHGTTIVALTFAGGVVMAGDRRATMGNLIANRDMDKVFEADEYSVVGIAGTAGL
                     AIELVKLYQVELEHYEKIEGAIMSLEGKANRLAGMIRANLAMAMQGLSVMPVLAGYDL
                     GDGSGRIFSYDVTGGCYAERDHHSIGSGSLFARGALKKVWQPGLSADAGVRAAVEALY
                     DAADDDSATGGPDLGRRIWPRVAVVDAHGVSFVTEDVLAGVVADLVSARRDNPGGAR"
     gene            complement(1678..>1866)
                     /locus_tag="VHM65_09690"
     CDS             complement(1678..>1866)
                     /locus_tag="VHM65_09690"
                     /inference="COORDINATES: protein motif:HMM:NF017455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HEX2706019.1"
                     /translation="GRPQRREDVPDEVPEPAPVAPEASARREAMSEDIDSVLDEIDLV
                     LESNAEEFVRGFVQKGGQ"
BASE COUNT          269 a          666 c          660 g          271 t
ORIGIN      
        1 tgcctgcact gtatgacgtg tcgccgcagc tccggtggtt ctggaatcac gggccgcatg
       61 ttgttggcct acggcgcccc ggaaccgtcg ccggaggcgc tcagcacgtc gtgatgaccc
      121 gggttcgggt cgtcgaccga cgggacatcg gtcgtcgggc tgtcggcact gagcaggtcc
      181 tccagcaggg ggccgaggat ccggcggaag gcccgccggg ggcggttgcg gtcgagcacg
      241 gcgacctcga gggcgtccgc gccgaggggg cgcggcgctc cgccggccgg gtcagccgcg
      301 agcacctcga cagcgaggtc gaggacgtcg gccatcgtca gacccggctc ccaggcctcg
      361 gccaaccgct tctcggcggc ctcgctcgct ccgcccatcg cgacgaaacc acgctcgtcg
      421 gcgaccgacc cgtcatacgt gagccggtag atctggtcct ggtcggggct gtgccccacc
      481 tcggcaacga cgatctcgac ctcgtagggc ttggactcct gggtgaagac cgcaccgagg
      541 gtctgggcgt aggcgttggc caggccgcgg gccgtcacgt cggagcggtc gtaggagtag
      601 ccccgcaggt cggcatagcg gacgccggcg acgcgcaggt tctcgaactc gttgtacttg
      661 cccacggcgg cgaaggcgat gcggtcgtag atctccgaga tcttgtgcag cgtccgcgag
      721 gggttctcgg cgacgaacgc gatcccgctg tcgaagccca tggcgacgac catccggccg
      781 cgggcaatcc ccttgcgcgc gtagtccgcc cggtccttca tgagctgctc gggcgagacg
      841 tagaagggca tcgtcaccgg gcacctcccg ggttgtcccg gcgcgccgac acgaggtccg
      901 cgacgacccc ggcgagcacg tcctcggtga cgaacgacac cccgtgggcg tcgacgaccg
      961 ccacccgggg ccagatccgg cgcccgaggt ccgggccgcc cgttgccgag tcgtcgtcgg
     1021 ccgcgtcata cagtgcctcg accgcagccc ggacccccgc gtcggcactc aacccgggct
     1081 gccacacctt cttgagcgcc ccgcgggcga acagcgaccc ggagccgatg ctgtgatggt
     1141 cgcgctcggc atagcacccg cccgtgacgt cgtaggagaa gatccgcccg ctcccgtcgc
     1201 cgaggtcgta gccggcgagc accggcatga ccgacagccc ctgcatggcc atggcgaggt
     1261 tggcccggat catgccggcg aggcggttgg ccttgccctc gagggacatg atcgcgccct
     1321 cgatcttctc gtagtgctcc agctcgacct gatagagctt gacgagctcg atcgcgagcc
     1381 cggccgtccc ggcaataccc accacggagt actcgtcggc ctcgaagacc ttgtccatgt
     1441 cccggttggc gatgaggttg cccatcgtgg cccgccgatc accggccatg acgaccccgc
     1501 cggcgaaggt cagcgcgacg atggtcgtcc cgtgcggcgc ctcggggacg acgccggcgg
     1561 gcagcgggcg gcccgcgggg agcagcgagg gagcctgcgc cgcgacgaag tcggtgaagg
     1621 aggacgaacc cacctgccgg tatggcgcgg gcaggctgcc agcgaagcct ccggtggtca
     1681 ctggccgccc ttctggacga agccgcggac gaactcctcg gcgttggact cgaggaccaa
     1741 gtcgatctcg tcgagcaccg agtcgatgtc ctcgctcatg gcctcacgcc gcgcgctcgc
     1801 ctccggagcg acgggggcgg gctcgggcac ctcgtcgggc acgtcctcgc gccgctgcgg
     1861 acgccc
//
LOCUS       DATAIX010000659         1658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_137725, whole genome shotgun sequence.
ACCESSION   DATAIX010000659 DATAIX010000000
VERSION     DATAIX010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137725"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="VHM65_09695"
     CDS             complement(<1..957)
                     /locus_tag="VHM65_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX2706020.1"
                     /translation="MTTELTRSAEAPAREVDLAITGMTCASCSARIERELNRLDGVQA
                     NVNLATEKAHIRYAGPLRVTDLLATVEAAGYGAAIVEAAAPPQPHELPGVALRRRLAV
                     AAVLTVPVFALAMVIPAFPASPWLQLLLATPVVTWAAYPFHRAAVINARHRSSTMDTL
                     VSLGVIAAYGWSLVTLLWNTTLDLGDGGAVEMSPAMAGQGHLYFEVAAVVTTFLLLGR
                     TLEARARSAGRQALESLADLGATTVAVLRSHPQTGAETEDTIRIEDLAVGDRFVVRPG
                     EKVATDGVVADGHSALDTSIITGESLPVDVAPGDRVTGGTVNT"
     gene            complement(954..1190)
                     /locus_tag="VHM65_09700"
     CDS             complement(954..1190)
                     /locus_tag="VHM65_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEX2706021.1"
                     /translation="MTMTTLTVTGMTCGHCTSAVTQELTALPGVRDVAIDLVAGGASP
                     VTITSDDPLDREAVVEAVAEAGYTAAFGSDAEVS"
     gene            complement(1236..1517)
                     /locus_tag="VHM65_09705"
     CDS             complement(1236..1517)
                     /locus_tag="VHM65_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002530052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX2706022.1"
                     /translation="MAGYTGTKDDYLRRLRRIEGQVRGLQRQVAEDTYCIDVLTQISA
                     VTKALQAVSLGLLEDHMVHCVRDAARDSDEAGAAKVAEATAAIARLVRS"
BASE COUNT          225 a          542 c          648 g          243 t
ORIGIN      
        1 ggtgttgacg gtgccaccgg tgacgcggtc gccaggagcg acgtcgaccg ggagggactc
       61 gccggtgatg atcgaggtgt cgagcgcgct gtggccgtcg gcgaccacgc cgtcggtggc
      121 gaccttctca ccggggcgca cgacgaagcg gtcgccgacg gcaaggtcct cgatccggat
      181 cgtgtcctcg gtctcggcgc ccgtctgcgg gtgtgagcgc aggacggcga ccgtcgtggc
      241 cccgaggtcg gccagcgact cgagcgcctg ccgcccggcc gatcgggccc gggcctcgag
      301 ggtccgcccc aggagcagga acgtggtgac gaccgcggcg acctcgaagt agaggtggcc
      361 ctgcccggcc atcgcggggc tcatctccac cgccccgccg tccccgaggt cgagggtggt
      421 gttccacagc agggtgacca gcgaccagcc gtaggccgcg atgactccga gggacacgag
      481 cgtgtccatc gtcgaggaac ggtgccgtgc gttgatgacc gcggcgcggt ggaaggggta
      541 ggccgcccac gtcacgaccg gcgtggcgag gagcagctgc agccaggggc tcgccgggaa
      601 ggccgggatg accatggcga gcgcgaagac cgggacggtc aggacggcgg cgacggcgag
      661 gcggcggcgc agcgcgaccc cgggcagctc gtgcggctgt ggcggggcgg ccgcctcgac
      721 gatggccgcg ccgtacccgg ccgcttcgac ggtggcgagc aggtccgtga cccgcagcgg
      781 cccggcatac cggatgtgcg ccttctcggt ggccaggttg acgttggcct ggaccccgtc
      841 gagcctgttg agctcgcgtt cgatgcgagc cgagcaggag gcgcaggtca tgccggtgat
      901 cgcgaggtcg acctcgcgcg ccggtgcctc ggccgagcgg gtgagctccg tcgtcatgac
      961 acctcggcgt cagaaccgaa ggcggcggtg tagcccgcct ccgcgaccgc ctcgacgacg
     1021 gcctcgcggt cgagcgggtc gtcgctcgtg atcgtgacgg gggaggctcc gcccgcgacg
     1081 aggtcgatgg cgacatcgcg gacgcccggc agcgcggtga gctcctgggt gaccgcgctc
     1141 gtgcagtggc cgcacgtcat gccggtcacg gtcagggtgg tcatggtcat ggtggtctcc
     1201 ctcgatggtg ggttcggctg cgggtcggcg gcgggtcagc tgcggacgag gcgggcaatc
     1261 gcggcggtgg cctcggcgac cttggccgcg ccggcctcgt cggagtcccg ggccgcgtcg
     1321 cggacgcaat ggaccatgtg gtcctcgagc aggcccaggc tgacggcctg cagcgccttg
     1381 gtgacggcgc tgatctgggt gagcacgtcg atgcagtagg tgtcctcggc gacctggcgc
     1441 tgcaggccgc gcacctggcc ctcgatgcgg cgcagtcgcc tgagatagtc gtccttggtg
     1501 ccggtgtagc ccgccatggg tcctcctcgg tgaccggttc gagtcaacta tacccccatg
     1561 gggtatgacg agcagccgct cgcgaccacc cggacaggtc tatgcttcgc gcaagggtgc
     1621 caatgaggac aaagtgggca caatgtcggg gtttgacc
//
LOCUS       DATAIX010000660         1725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_137734, whole genome shotgun sequence.
ACCESSION   DATAIX010000660 DATAIX010000000
VERSION     DATAIX010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137734"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..610)
                     /locus_tag="VHM65_09710"
     CDS             complement(158..610)
                     /locus_tag="VHM65_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX2706023.1"
                     /translation="MEALDRRLVDLLAADGRMSFTDLGKATGLSTSAVHQRVRRLEER
                     GVITGYQAIVSPEALGLTLTALISVTPLDPAAPDDVPDRLEGISELEACHSVAGDDSY
                     VLKARVGTPSDLEALLARIRAVANVSTRTTVVLSTPWEGRPASSASSA"
     gene            703..1641
                     /locus_tag="VHM65_09715"
     CDS             703..1641
                     /locus_tag="VHM65_09715"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HEX2706024.1"
                     /translation="MTTPARLMLLDSASLYFRAFYGVPDTRTDPAEPPTNAVRGFLDM
                     IASLVERYRPTHLVACWDDDWRPAFRVAAIPSYKAHRVSLVDPAGVVEESPEDLTPQV
                     PVIVEALAALGIARVGAPGYEADDVIGTLTHQWRGLGPVDVVTGDRDLFQLVDDSVGV
                     RVLYTARGGVRDPDLVDETFLRDKYAVTSGSAYADMAALRGDTSDGLPGVAGVGEKTA
                     ATLIARYGDLATLRAAVASGDPLIKGAQRARLEAASAYLDVAPLVVRVALDVPIGPVD
                     AVLPSSVADASLMSRLAVDYGLTSSFNRVIASLNLA"
BASE COUNT          230 a          630 c          609 g          256 t
ORIGIN      
        1 ctgcaggcgt gtgtcacgac aggggtcaac gccctggtcg ccggggcggt ctcgccctcg
       61 gtcagcgcct cgatgcgcgg gctggcggtg acctctgcgg caagcacgct ggtggcctgg
      121 tctgtctggg cctggcaccg cagggcggcc cgggcggcta cgccgacgac gccgacgacg
      181 ccggacggcc ttcccacggg gtcgagagca ccacagtcgt cctcgtcgac acgttcgcca
      241 cggcccggat ccgggccagc agcgcctcga ggtcgctcgg cgtccccacc cgggccttga
      301 ggacgtagct gtcgtcgccc gctacggagt ggcaggcctc gagctcggag atgccttcga
      361 gccggtcggg cacgtcatca ggggcggccg ggtccagtgg cgtcaccgag atgagcgcgg
      421 tgagggtgag gccgagcgcc tcggggctga caatcgcctg gtagccggtg atgacgccgc
      481 gctcctcgag acggcgcacc cgctggtgca ccgccgatgt ggacagcccc gtcgccttgc
      541 ccaggtcggt gaagctcatc cgcccgtcgg cggcgagcag gtccacgaga cgtcggtcga
      601 gtgcttccac gggctacagg ctagacgcag gaggaccagg gcggctgtcc catatcaccg
      661 acagaccctc cggcaggccg ccgcggatgc gacgatgcct gcgtgacgac gcccgcacgc
      721 ctcatgctcc tggactccgc gagcctgtac tttcgcgcct tctatggcgt ccccgacacc
      781 cggaccgacc ccgcggagcc gcccaccaac gccgtccgcg gcttcctcga catgatcgcc
      841 tccctcgtcg agcgctaccg cccgacccac ctcgtcgcgt gctgggacga cgactggcgg
      901 cccgccttcc gggtagccgc gatcccgtca tacaaggcgc atcgggtctc cctcgtggac
      961 ccggccggcg tggtggagga gtcacccgag gacctgaccc cgcaggtgcc ggtcatcgtc
     1021 gaggcgctgg ccgcgctcgg gattgcccgc gtcggcgccc cgggctacga ggccgacgac
     1081 gtcatcggga ccctgaccca ccagtggcgc ggtctcggcc cggtcgacgt cgtgaccggt
     1141 gaccgggacc tcttccagct cgtcgacgac tcagtcggcg tgcgcgtgct ctataccgcg
     1201 cgtggcggcg tgcgtgaccc ggacctcgtc gacgagacct tcctgaggga caagtatgcc
     1261 gtcacctcgg gcagcgccta cgcggacatg gccgccctgc gtggcgacac gagcgacggc
     1321 ctgcctggcg tggccggggt gggcgagaag accgccgcga cgctcatcgc ccggtatggc
     1381 gacctggcca cgctgcgcgc cgccgtcgcc tcgggcgacc cgctgatcaa gggggcgcag
     1441 cgcgcgcggc tcgaggccgc gtcggcatac ctcgatgtcg ccccgctcgt cgtccgcgtg
     1501 gctctggacg tcccgatcgg acctgtcgac gcggttctgc cgagctcggt ggcggacgcg
     1561 tcactgatga gccgactcgc cgtcgactac ggcttgacct cgagcttcaa ccgcgtcatc
     1621 gccagcctga acctggcctg acgcgcctcg accggacttc tgcgcgcggg aagtcgccac
     1681 agaggagggt tttggcgcca gagcggccgg agggggggtt tgacg
//
LOCUS       DATAIX010000661         3150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_291662, whole genome shotgun sequence.
ACCESSION   DATAIX010000661 DATAIX010000000
VERSION     DATAIX010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291662"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VHM65_09720"
     CDS             <1..494
                     /locus_tag="VHM65_09720"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX2706025.1"
                     /translation="YTWFASANDNDDVVFRTLSFVVMAGAPLLAAGVPDIFADGQSVV
                     MVIGYAIMRLAMVALWLRAAAGHPQRRATALTYTLGIVVAQVFWIARLAIGNPAWFVP
                     TFLLGMALEQSVPALAERRGAHPVPPTPHRPALCAAYPHRARSRRSRRSRRSRRSRRC
                     KVP"
     gene            765..1010
                     /locus_tag="VHM65_09725"
     CDS             765..1010
                     /locus_tag="VHM65_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706026.1"
                     /translation="MALCLSLFTLALSGIHAAGGRDGQGPMAVPTALAVLILVIALVG
                     LSMGMTVLLIGLAMTAVVAGNIAAGNRALVGYPSRLV"
     gene            1242..1523
                     /locus_tag="VHM65_09730"
     CDS             1242..1523
                     /locus_tag="VHM65_09730"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX2706027.1"
                     /translation="MRATWNGTVIAESEDTVVVEGNHYFPLEAVRQDALRPSQTHTTC
                     PWKGIASYYSLQVDGQANPDAVWFYPEPKDAAAQITGRVAFWRGVTVGP"
     gene            1620..2282
                     /locus_tag="VHM65_09735"
     CDS             1620..2282
                     /locus_tag="VHM65_09735"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX2706028.1"
                     /translation="MPSLPIFPLGTVLLPGAPLPLQLFEPRYLTMLQELMALPEQSRV
                     FGVVALRRGHEVGPEAALDLHEVGCAARVEAVAAVPSPGGPRYHILAVGTRRFRLEQI
                     DPRAQTPYLTGRVTWLGEPEGPDGAAAALAVRVAAEHAAYRRVLGAEPVALPEDPGAL
                     AYRVADSTVLALAERQRVLAAPDTAARLRLVGHLLRRERTLVEQFRAVPQTPDLGGGG
                     LN"
     gene            2315..>3150
                     /locus_tag="VHM65_09740"
     CDS             2315..>3150
                     /locus_tag="VHM65_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2706029.1"
                     /translation="MSTNATDHAYAPPGRAVEVSDGIYAYVQPDGSWWINNTGFFVGR
                     RGVVTVDACATEARTRAYLKAIRAVTDAPVTTVLNTHHHGDHTFGNFLFPGATIVGHD
                     NVRAALLAWGLPRAAPFWTDVDWGAVELAPPFLTYSEGVTLHVDDLRCEVRYAGTPAH
                     TTNDSFVWVPERRLLFSGDLLFNGGTPFLVQGSIAGALVALGVLRGLGAETIVPGHGP
                     VCGPEVIERVASYLRFVQDTARRGRAAGLSPLEVARESSLGQFAGWLDPERIVGNLHR
                     AYA"
BASE COUNT          405 a         1129 c         1105 g          511 t
ORIGIN      
        1 actacacctg gttcgcctcg gccaacgaca acgacgacgt cgtcttccgc accttgagct
       61 tcgtcgtcat ggctggggcg ccgctgcttg ctgcgggggt gcccgacatc ttcgccgacg
      121 gccagtcggt ggtcatggtg atcggctacg cgatcatgcg tctcgccatg gtcgctctgt
      181 ggctccgggc cgcggccggg catccccagc gacgggccac tgctctgacg tataccttgg
      241 gtatcgttgt ggcgcaggtg ttctggatcg cccggctggc gatcggcaac cccgcgtggt
      301 tcgtcccgac cttcctgctc ggtatggcgc tcgagcagtc cgtgcctgcc ctcgccgagc
      361 gccggggggc acaccccgtt ccacccacac cacatcgccc agcgttatgc gctgcttacc
      421 ctcatcgtgc tcggtccagg cggtccaggc ggtccaggcg gtccaggcgg tccaggcggt
      481 gcaaggtgcc atgaggctgc acggtgggat cgggggcagt ggtgtctccg gtgagctgct
      541 catgctcatc atcggcggtc tgctcgtcgt cttctcgctg tggtggatct acttcaacag
      601 cgacatggcc gacatcgtga cgggggagaa gtcggtgtgg ttcttcgggt acgggcactt
      661 tgtggtcttc gcgtccatcg ccgccctcgg cgcggcgttg gccgccgcgg tcgacgtcgt
      721 gcagcacgcc gcccacgtcg ggccgcgccc gggtcggcat cgcggtggcg ctgtgcctgt
      781 cgctgttcac cctcgcgctg tccgggattc acgcggccgg cggccgggac gggcagggcc
      841 cgatggccgt accgacggct ctcgcagtcc taatcctcgt catcgccctc gtcgggctgt
      901 cgatggggat gaccgtgctc ctcatcggcc tggccatgac cgccgtggtg gctggcaaca
      961 tcgccgctgg caaccgagca ctcgtcggtt acccctctcg gctggtgtga cgacataccc
     1021 ctctcgttga gtgagcgcag cttgccctgg agagcgcgta cttgaggcaa agtgcgctca
     1081 ctcaacggag ggctgctggc gaagcccgct acctcgccac caaccgcact tcccggcgcg
     1141 ggcggtgggg ctgatgcctg ccggcaggca tcagctcagt cctgcgaggc gtgcgaccct
     1201 gggggtgccg acctcaccac cgccctccga gggagcaccc catgcgcgcc acatggaacg
     1261 ggaccgttat cgccgagagt gaggacaccg tcgtcgtcga gggcaaccac tacttccccc
     1321 tggaggcggt gcgtcaggac gccctgcgac cctcgcagac tcacaccacc tgcccgtgga
     1381 agggcatcgc gtcctactac tcgctccagg tcgacgggca ggcgaacccc gacgcggtct
     1441 ggttctatcc cgagcccaaa gacgcggcgg cccagatcac ggggcgggtc gccttctggc
     1501 gaggggtgac cgtcgggcca tagccgcgag tctggccctg accgggcccc cgacgggcgg
     1561 cgcccggatg aggcgacccc cgacgggact gccctccgcg cgcgacatac cctgccggta
     1621 tgccgtcgct ccccatcttc ccgctgggca cggttctctt gccgggggcc ccgctgccgc
     1681 tccagctgtt cgagccgcgc tacctgacga tgctgcagga gctgatggct ctgccggagc
     1741 agagccgggt gttcggggtc gtcgcgctgc ggcgtgggca cgaggtcggc ccggaggcgg
     1801 cactcgatct gcacgaggtc gggtgcgcgg cgcgggtcga ggcggtcgct gcggtcccgt
     1861 ccccgggcgg cccgcgatac cacatcctcg ccgtcgggac gcggcggttc aggctcgagc
     1921 agatcgaccc gcgggcgcag acgccctatc tcaccggccg ggtgacctgg ctcggtgaac
     1981 ccgaggggcc ggacggagcc gcggcggcac tggcggtccg agtcgctgcc gagcacgcgg
     2041 cataccggcg ggtcctgggc gccgaacccg ttgccctgcc agaggatccg ggcgcgctcg
     2101 cctaccgggt ggccgactcg acggtgctcg ccctggccga gcggcagcgg gtcctggccg
     2161 ccccggacac cgccgcgcgg ctgcggctcg tcgggcacct cctgcgccgc gagcggacgc
     2221 tcgtcgagca gttccgcgcg gtgccccaga cccccgacct cggcggcggg ggcctcaact
     2281 gagccaaccc ggcggcgaag ggccacaatc gggcatgagc acgaacgcca ccgaccacgc
     2341 ttacgccccg ccgggacggg ccgtcgaggt gagcgacggg atctacgcct atgtccagcc
     2401 cgacggcagc tggtggatca acaacacggg gttcttcgtc gggcgccgtg gggtcgtcac
     2461 tgtcgacgcc tgtgccactg aggcccgaac tcgcgcgtat ctcaaggcga ttcgggcggt
     2521 caccgacgcg ccggtgacga cggtgctcaa cacgcaccac cacggggacc atacgttcgg
     2581 caacttcctc ttccccggcg ccacgatcgt cgggcatgac aacgtccggg cagcgctgct
     2641 cgcgtggggc ctgccgcggg cggcgccgtt ctggaccgac gtcgactggg gtgcggtcga
     2701 gctggccccg ccgttcctca cctactccga gggggtgacg ctccatgtcg acgacctccg
     2761 gtgcgaggtc cggtatgccg gcacgcccgc ccacacgacg aacgactcgt tcgtctgggt
     2821 cccggagcgg cggctgctct tcagcgggga tctgctcttc aacggcggga cacccttcct
     2881 cgtgcagggc tcgatcgccg gggcgctcgt ggcgctcggc gtgctccgcg gcctcggcgc
     2941 cgagacgatc gtgccgggtc acgggccggt ctgcgggccc gaggtgatcg aacgggtcgc
     3001 gtcatacctg cgcttcgtcc aggacaccgc gcggcgaggg cgagcggccg ggctctcgcc
     3061 gcttgaggtc gcccgggaga gcagcctcgg gcagttcgcc ggctggctcg accccgagcg
     3121 gatcgtcggc aacctgcacc gcgcgtatgc
//
LOCUS       DATAIX010000662         3083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_291706, whole genome shotgun sequence.
ACCESSION   DATAIX010000662 DATAIX010000000
VERSION     DATAIX010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291706"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VHM65_09745"
     CDS             complement(<1..507)
                     /locus_tag="VHM65_09745"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HEX2706030.1"
                     /translation="MFFWREDVPRAGGHGGYTWGFTSAPGGHSRPPYDGWNLGAHVGD
                     DPAAVRANRAALAERLGVREPELTFMNQVHGAAVARVGPSGWLGPAPACDAVVTAAAG
                     RALVVLVADCAPVLLVDPVSGLCAAVHAGRRGLVSRVVPRAVEALRSLGSRDLRAVVG
                     PSICAGCYE"
     gene            complement(532..1731)
                     /gene="ftsZ"
                     /locus_tag="VHM65_09750"
     CDS             complement(532..1731)
                     /gene="ftsZ"
                     /locus_tag="VHM65_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX2706031.1"
                     /translation="MVATPQNYLAVIKVVGIGGGGVNAINRMIDVGLKGVEFIAINTD
                     AQALLMSDADVKLDVGRELTRGLGAGADPEVGKRAAEDHAEEIEEVLQGADMVFVTAG
                     EGGGTGTGGAPVVARIAKGLGALTIGVVTRPFTFEGRRRANQAETGIGGLREEVDTLI
                     VIPNDRLLSISDRTVSMMDAFRSADQVLLSGVQGITDLITTPGLINLDFADVKSVMKG
                     AGSALMGIGSSRGEDRAVQAAELAISSPLLEASIDGAHGVLLSISGGSDLGLFEINEA
                     ARLVQEAAHPEANIIFGAVIDDALGDEVRVTVIAAGFDGGAPVRRQDDRALGQIHSGR
                     QAVVTPAGAPVSASAETRPAPATPTFTPSYPPPVPAEPTPAELVRPPRTVTFDEPEEL
                     DVPDFLK"
     gene            complement(2027..2788)
                     /locus_tag="VHM65_09755"
     CDS             complement(2027..2788)
                     /locus_tag="VHM65_09755"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX2706032.1"
                     /translation="MSRPGFHGSRSPRPSPARDSRGLFEARAAAVRRGRLLRLSRSGA
                     VVVLLIALIWLVAFSPLLASRSVVVSGVSGAPRAAVTAAAAVPLGVPLARLDIAAIQL
                     RVKALPTVDTVTVSRSWPTTVLITATLKKPAIVLQNSQGQLDVVAPDGLVIERVTAAP
                     DGLPVVRGGAQAPTPEGIAAALGFVKVLSAAQRKQVSEITVSASFVTARWGTLKVVWG
                     DGSRAGTKARLLTILLATKPASLIDVSAPDTPVTR"
     gene            complement(2785..>3083)
                     /locus_tag="VHM65_09760"
     CDS             complement(2785..>3083)
                     /locus_tag="VHM65_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706033.1"
                     /translation="TRDFATELGRALSVADVLVVMDVYGAREDPIPGVSGALVAVAAR
                     AARPGAMVRYEPDAEVVASALAALVRPGDLCLTVGAGDVTRVGPALLDLLGART"
BASE COUNT          452 a         1088 c         1129 g          414 t
ORIGIN      
        1 ctcgtaacac cccgcacaga tgctgggacc caccaccgct cgcaggtcac ggctccccag
       61 gctgcgcagc gcctcgacgg ctcgggggac gaccctcgag acgagcccgc gtcgcccggc
      121 gtgaacagcg gcgcagagcc cgctcaccgg gtcgaccagc aagacggggg cacagtcggc
      181 cacgaggacg acgagcgcgc gcccggccgc cgcggtgacc acggcgtcac acgccggcgc
      241 gggtcccagc cagcccgatg gcccaacccg ggcgaccgcg gcgccgtgga cctggttcat
      301 gaaggtcagc tcgggttccc tgactccgag gcgctcggcg agggccgcgc ggttcgctcg
      361 gactgccgcg gggtcgtccc cgacgtgcgc gccgagattc cagccgtcat acgggggccg
      421 gctgtgccct ccgggagcgg aggtgaaacc ccaggtgtag ccgccgtgcc cccctgcccg
      481 gggcacgtcc tcgcgccaga agaacacggt gccggtcccg ggcggccggt cctacttgag
      541 gaagtcgggg acgtcgagct cctccggctc gtcgaaggtc accgtccgcg gcggacgaac
      601 cagctcggcc ggggtgggct cggcggggac cggcggcggg tatgacgggg tgaaggtcgg
      661 cgtcgcgggc gccggcctgg tttcggccga ggcagaaacg ggcgcgccgg ccggcgtcac
      721 cacggcctgg cgaccggagt ggatctgccc cagggcccgg tcgtcctggc ggcgcaccgg
      781 cgcgccgccg tcgaacccgg cggcgatgac ggtgacgcgg acctcgtcgc cgagcgcgtc
      841 gtcgatgacc gcgccgaaga tgatgttggc ctcggggtgg gcggcctcct ggacgagccg
      901 ggccgcctcg ttgatctcga acaggcccag gtcggatccg ccggagatgg acagcaggac
      961 gccgtgggcg ccgtcgatcg aggcctcgag cagcggcgag gagatcgcga gctcggccgc
     1021 ctggaccgcg cggtcctcac cgcgggacga gccgatgccc atgagcgccg accccgcgcc
     1081 cttcatgacc gacttgacgt cggcgaagtc gaggttgatc agacccgggg tcgtgatgag
     1141 gtcggtgatg ccctggacac cggagagcag gacctggtcg gccgagcgga aggcgtccat
     1201 catcgacacg gtgcggtcgc tgatcgacag cagccggtcg ttggggatga cgatgagggt
     1261 gtcgacctcc tcccgcaggc cgccgatgcc cgtctcggcc tggttggcgc ggcggcgccc
     1321 ctcgaaggtg aacggccggg tcaccacacc gatggtcaag gcgcccaggc ccttggcgat
     1381 ccgcgcgacg accggcgcac caccggtgcc cgtgccgccg ccctcgcccg ccgtgacgaa
     1441 gaccatgtcg gccccctgga ggacctcctc gatctcctcg gcgtggtcct ctgcggcccg
     1501 cttgccgacc tcggggtccg ccccggcgcc caagccgcgg gtcagctcgc ggccgacgtc
     1561 gagcttgacg tcggcgtcgc tcatcagcag ggcctgggca tcggtgttga tcgcgatgaa
     1621 ctcgaccccc ttgagcccga cgtcgatcat ccggttgatg gcgttgacac cgccaccacc
     1681 gatcccgacg accttgatga cggccaagta gttctgcggg gtagctacca cggcggtggg
     1741 cctctctcag tgacgttcat cggtcgcaac ttgtcccgca cggggtggtc tcgacacgcc
     1801 ggccgagggc cggaccgggg tcaatctcac cttgagcgtt actgttctgc tgggtccttc
     1861 tgagagggga cgctatgcgt catacccagg ctgggcaacg atgcggaccg gcgtgtccgc
     1921 gatcgggtca ccactttccc tcgtcaaggc cgtccgacct gtggcacgtg cgagtcatgg
     1981 gcagggtgag gtaagtgtga ctggcgcgac tgatgtgacg gcggtctcag cgagtgacgg
     2041 gcgtgtcggg cgcgctgaca tcgatgagcg acgccggctt ggtggcgagc aggatggtga
     2101 gcagccgggc cttggtcccg gcgcgtgagc cgtcgcccca gaccaccttc agtgtccccc
     2161 accgggcggt gacgaaggag gcggagacgg tgatctctga gacctgcttg cgctgggccg
     2221 cgctgagcac cttgacgaag cccagcgcgg ccgcgatgcc ttcgggggtc ggggcctggg
     2281 cgcccccgcg gaccaccggc aacccgtccg gtgccgccgt cacccgctcg atgaccagcc
     2341 cgtcaggagc cacgacgtca agttgacctt gagagttctg caggacgatc gcgggcttct
     2401 tgagggttgc ggtgatgagg accgttgtcg gccacgaccg tgacacggtg acggtgtcga
     2461 cggtcggcag tgccttgact cggagctgga tcgcggcgat gtcgagtcgc gcgaggggca
     2521 cgccgagggg gaccgcggcg gcggcggtca cggccgcgcg cggggccccg ctcacgcccg
     2581 agaccacgac cgacctgctc gccaggagcg ggctgaaggc gacgagccag atcagggcga
     2641 tgagcaggac caccacggca ccggaccggc tcaggcgcag gaggcgcccc cggcgcacgg
     2701 ccgccgcccg agcctcgaag agcccgcgcg agtcccgcgc cgggctcggc cgggggctcc
     2761 tcgacccatg gaacccgggc ctgctcatgt ccgggctccg agtaggtcga gcagcgcggg
     2821 gccgacccgc gtgacatcac cggctcccac cgtgagacac aggtcacccg gccggaccag
     2881 agcggcgagc gccgatgcca ccacttccgc gtcgggctca taccggacca tggcaccggg
     2941 acgagccgcg cgggcggcga cggcgaccag cgccccgctg acacccggta tcgggtcctc
     3001 gcgggcgcca tacacgtcca tcacgacgag gacgtcagcc accgagagcg ctcggcccag
     3061 ctcggtcgca aagtcccggg tcc
//
LOCUS       DATAIX010000663         1817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_291716, whole genome shotgun sequence.
ACCESSION   DATAIX010000663 DATAIX010000000
VERSION     DATAIX010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291716"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..217)
                     /locus_tag="VHM65_09765"
     tRNA            complement(135..217)
                     /locus_tag="VHM65_09765"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(182..184),aa:Leu,seq:cag)
     gene            508..1362
                     /locus_tag="VHM65_09770"
     CDS             508..1362
                     /locus_tag="VHM65_09770"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HEX2706034.1"
                     /translation="MGLFDRVERGLERAVSGAFAKAFKSEVQPVEIAAAIRRRMDDRA
                     ALLGHGRTIVPNLYTVELSPTDFERLAGFEEALSEELVASAEEHVDTQRYTPGGPVQV
                     LFSKGEDLETGVFRVGSATTKSRPTTPRHEPASEYEAQPAPGLATAASPPVAAPATSA
                     DRPDPIPTAAPSPAHEPAHRSTYVNPAERPWLDVDGDRYPLIGAISVVGRDASADIVL
                     NDPGISRRHSEIRVTNDGPHLVSSIRDLASTNGTFVNGDRITSRRLQDGDAVTVGRTT
                     VTFHAGTR"
     gene            1414..>1817
                     /locus_tag="VHM65_09775"
     CDS             1414..>1817
                     /locus_tag="VHM65_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX2706035.1"
                     /translation="MSELTVTLVRMGLLALLWIFVFSIVGVVRTDIYGTPVSRRKSKP
                     ARDKSTAKAAKKAKAGGPAPTAVTPYEPAKPRKGEPRTLTVTDGPLAGTTFPLKPSGV
                     LIGRNPECTLVVDDDFTSGRHARLSARPDGWYA"
BASE COUNT          303 a          715 c          571 g          228 t
ORIGIN      
        1 tcgtacggtc ggcgcgccga cgaggggccg gcgctgggca gccaccaccc ccgaacgccg
       61 agaacctccg gccaggtcgc cctcggccgg gagttctctc ggtacaacac gggtgaagac
      121 ttgatcaccc cgggtgcgca aggggggatt tgaaccccca cgcccttgca ggcactggca
      181 cctgaagcca gcgcgtctac cattccgcca cttgcgcgtg tgcggtcaca gtgtgctgca
      241 tccgcgccag gttcgccaaa ctgtatggcg cgtgcatcct cccgccgccg agccggcagc
      301 gccaagccga ccacacttca cgcgctccca cctcttcgct gccgcccacc gcgggcacgg
      361 catagcctct cgcgcgcgct cgcagcggcc tcggcccggg catcgcgacg caccaggaac
      421 cgccctcaca ccccgctcgt tgccagcgag tccgcgagcg ggccggatac catgacaagt
      481 caagacgcga cggtgaagga gatgcccgtg ggactgttcg accgcgtgga gcggggcctc
      541 gaacgggccg tgagcggcgc cttcgccaag gccttcaagt ccgaggtcca gcccgtcgag
      601 atcgccgccg ccatccgccg ccgcatggac gaccgcgccg ccctgctcgg ccacggccgc
      661 accatcgtgc cgaacctcta caccgtcgag ctcagcccga ccgacttcga gcgcctcgcc
      721 ggcttcgagg aggccctgag cgaggagctc gtcgccagcg ccgaggagca tgtcgacacc
      781 cagcgctaca cccccggggg cccggtgcag gtcctcttca gcaagggcga ggaccttgag
      841 accggcgtct tccgagtcgg ctccgcgacc accaagtcgc gccccactac ccccaggcac
      901 gagccggcca gcgagtatga ggcccagccg gcacctggcc tcgccacggc ggccagtcct
      961 cccgtcgccg caccggccac cagtgcagac cggccagacc cgatccccac cgcggcaccc
     1021 agccccgccc acgagccggc gcaccgctcg acgtatgtca acccggccga gcgcccgtgg
     1081 ctcgacgtcg acggcgaccg ctacccgctc atcggcgcga tcagcgtcgt cggccgtgac
     1141 gcgagcgctg atatcgtcct gaacgacccg ggaatctcca ggcgccacag cgagattcgg
     1201 gtcaccaacg acggccctca cctggtcagc tcgatccgcg acctcgcctc gaccaacggc
     1261 accttcgtca acggagaccg catcacgagc cgccgcctcc aggacggcga cgccgtgacg
     1321 gtggggcgca cgacggtgac ctttcacgcc gggacccgtt aggcgtggcg cgcgtcaccg
     1381 tgcacacccc agaggaatcg gaatgatagg cagatgagcg agctgaccgt caccctcgtg
     1441 cggatgggtc tgctcgcgct gctctggatc ttcgtcttca gcatcgtcgg cgtcgtgcgc
     1501 acggacatct acggcacccc ggtcagccgc cgcaagagca agccggccag ggacaagagc
     1561 acggccaagg ccgccaagaa ggccaaggcg ggcgggccag cgccgacggc agtcacgcca
     1621 tacgagccgg cgaagccgcg caagggtgag ccgcgcacgc tgaccgtcac cgacggtccc
     1681 ctcgcgggca cgaccttccc gctcaagccc agcggggtgc tcatcggccg caaccccgag
     1741 tgcaccctcg tcgtcgacga cgacttcacc tccggccggc acgcgcgcct gtccgcccgc
     1801 cctgacggct ggtatgc
//
LOCUS       DATAIX010000664         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_201665, whole genome shotgun sequence.
ACCESSION   DATAIX010000664 DATAIX010000000
VERSION     DATAIX010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201665"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1905)
                     /locus_tag="VHM65_09780"
     CDS             complement(130..1905)
                     /locus_tag="VHM65_09780"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX2706036.1"
                     /translation="MGEHPRARRREEVAARRDELVDEPDIPGLGRAAPLALKQDLGER
                     SPAEARGIGLVDELVPSRGDLLPAARSWVLAQGGDVAAASQPWDRPGYRIPGGAPSDP
                     QLATILPALPATLRAQLKGAPYPAPRAILSAAVEGAQVDVDTASRIESRYFTSLATGP
                     ISTNLIQAFFFDMAAITSGSLRPAGIAPRRVARAGILGAGMMGAGIAHACARSGIEVV
                     LKDVSAEGAARGKAHSAALLDREVTRGRMSAVQRDAVLARITPTADAAGLAGCDVLVE
                     AVFEDVALKARVFAEAEAVVGPGTLLCSNTSTLPITGLAAAVARPADFIGLHFFSPVD
                     KMRLVEIIRGRATSDEAVARAVDLVAQIRKVPIVVHDGRGFYTSRVFMTLVTEGVALL
                     EEGVDPATLERAATMAGFPAPPLAMLDEVSLTLTQHIRGEAEAAAAEQGRILARPVGA
                     DTIDRMVGEFGRAGRAAGAGFYDYPAGAPKVLWPGLREHFTRSEVTIPLVDMAERYLF
                     VMALETAKCFEEGVLDSTAAANIGAILGIGFPPLYGGTAQYMHGYAGGLAGFVERARR
                     LASRYGDRFAPSAWLTAKAETGESW"
BASE COUNT          284 a          737 c          765 g          253 t
ORIGIN      
        1 cacccgtcgg tggctcgacc ttggtcaccg acgcgccgag ctcgaccagc cgggcggccg
       61 ccgccggacc gggcaggttc acggcaatcg tgacgactcg aatccccgcc aggggcaacg
      121 actccctgct caccacgact cccccgtctc ggccttggcc gtgagccacg cactcggggc
      181 gaaccggtcg ccataccggc tggcgagccg gcgggcccgc tcgacgaagc cggccagccc
      241 accggcatac ccgtgcatgt actgggcggt gccgccgtag agaggcggga agccgatgcc
      301 gaggatcgcg ccgatgttgg ctgcggcggt ggagtcgagc acgccctcct cgaagcactt
      361 ggcggtctcc agggccatga cgaacaggta gcgctccgcc atgtcgacca gcgggattgt
      421 gacctcggag cgggtgaagt gctcacgcag acctggccag agcaccttgg gcgcaccggc
      481 ggggtagtcg tagaagcccg ccccggcggc ccggccggcg cggccgaact caccgaccat
      541 ccggtcgatc gtgtcggcgc cgacaggccg cgccaggatg cggccctgct ccgctgcggc
      601 tgcctcggcc tcgccacgga tgtgctgggt cagcgtcagc gagacctcgt cgagcatggc
      661 cagcggcggg gccgggaagc cggccatcgt cgcggcgcgc tcgagcgtgg ccgggtcgac
      721 tccctcctcg agcagggcga caccctcggt gacgagggtc atgaagacgc ggctcgtgta
      781 gaacccgcgc ccgtcgtgga cgacgatcgg gaccttgcgg atctgggcca cgaggtcgac
      841 ggcccgggcg accgcctcgt cggaggtggc ccgcccgcgg atgatctcga cgaggcgcat
      901 cttgtcgacc ggcgagaaga agtgcagacc gatgaagtcc gccgggcgcg ccactgcggc
      961 ggcgagcccg gtgatgggca gcgtcgaggt gttggagcac agcagggtgc cgggtccgac
     1021 gacggcctcg gcctcggcga agacgcgggc cttgagcgcg acgtcctcga agacggcctc
     1081 gacgaggacg tcgcagcccg cgaggccggc ggcatcggcc gtcggggtga tccgggccag
     1141 gacggcgtcg cgctgtacgg cgctcatccg tccccgcgtg acctcgcggt cgagcagggc
     1201 ggccgagtgt gccttgcccc tggccgcgcc ctcggccgag acgtccttga ggacgacctc
     1261 gatccccgag cgggcacacg catgggcgat gcctgcgccc atcatgcccg cgccgaggat
     1321 tccggccctg gcgacccggc ggggggcaat cccggccggg cgcaacgagc cggacgtgat
     1381 ggccgccata tcgaagaaga aggcctggat caagttggtg ctgatcggcc ccgtggcgag
     1441 gctggtgaag tagcgggact cgatgcggct ggcggtgtcg acgtccacct gagcgccctc
     1501 gaccgccgcc gacaggatcg cccggggagc cgggtatggc gcccccttga gctgggcgcg
     1561 cagggtcgcc gggagcgcgg gcaggatcgt ggcgagctgg gggtcgctcg gggcgcctcc
     1621 ggggatgcgg tatcccgggc ggtcccacgg ctggctcgcc gccgcgacat cgccgccctg
     1681 ggcgagcacc cacgagcgcg ccgccgggag aaggtcgccg cgcgacggga cgagctcgtc
     1741 gacgagcccg atacccctgg cctcggccgg gctgcgctca ccgagatcct gcttcagggc
     1801 cagcggcgca gcccggccga ggccagggat atcgggctcg tcgacgagct cgtcccgtcg
     1861 cgcggcgacc tcctcccggc ggcgcgctcg tgggtgctcg cccatggcgg cgatgccgcg
     1921 gcggcgagcc agccgtggga ccgcccggga taccgcatcc ccggaggcgc cccgagcgac
     1981 ccccagctcg ccacgatcct gcccgcgctc ccggcgaccc tgcgcgccca gctcaaggg
//
LOCUS       DATAIX010000665         1636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_201824, whole genome shotgun sequence.
ACCESSION   DATAIX010000665 DATAIX010000000
VERSION     DATAIX010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201824"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1116)
                     /locus_tag="VHM65_09785"
     CDS             complement(13..1116)
                     /locus_tag="VHM65_09785"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2706037.1"
                     /translation="MSSPTTARPAGGRGRAVAVAGAASSVPGQIVSALTARALRRGGP
                     VSTVIGVDTVRGRAGSVQWRLADLRSPALASVLAGVDTVVWVACSTDLQRDLERRASE
                     RRAEVTRTALTVSMAAAAAGVSHLVVVTSAMVYGAAPDNPLPITEDSTRRATSDEGLV
                     GDLLAVEDVLERSRPDHPGLAITLVRPAALVGAGVDTVVTRHFAAPRLLAVKGTTPAW
                     QFCHVEDLASGVAETVARGLGPVVTVGSVGSMTQAEVERLSGMRRVELSESAALGTAA
                     RLHRIGVLPSPASDLSYALYPWVVSSETLRANAWEPAYDNATALGVLLDGVRGQYAIA
                     GRRVERKDAALGAASAAVALVGTAAILRRARRK"
     gene            1259..>1636
                     /locus_tag="VHM65_09790"
     CDS             1259..>1636
                     /locus_tag="VHM65_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX2706038.1"
                     /translation="MSDTPQDRGGEGPQDLSAMLSQFLGETAQNPELAEALRGMGIDP
                     SDPATLATLTTQMRSLFDPASAPDPAELATTAARAIVAAGDADPSLGAAARRAVADAV
                     GVAQLWVDPVTTLPASPGSGRAWS"
BASE COUNT          224 a          605 c          604 g          203 t
ORIGIN      
        1 cggtatggcg catcacttcc tcctcgcccg tcgcaggatg gcggcggtcc cgacgagtgc
       61 gacagctgcg ctggccgctc cgagggcggc gtccttgcgc tcgacccggc gcccggcgat
      121 ggcgtactgg ccccggacac cgtcgagaag caccccgagc gccgtcgcat tgtcgtaggc
      181 cggttcccac gcgttggcgc gcagggtctc gctggacacc acccaggggt agagcgcgta
      241 ggacaggtca ctggccgggg acgggagcac gccgatccgg tgcagccggg ccgcggtccc
      301 cagtgcggca ctctcgctca gctcgacccg gcgcatgccc gagagccgct cgacctccgc
      361 ctgggtcatc gagcccaccg acccgacggt gacgaccggg cccaggccgc gggccacggt
      421 ctcggcgaca ccggaggcca ggtcctcgac gtggcagaac tgccaggcgg gggtcgtccc
      481 cttgacggcg agcaggcggg gggccgcgaa gtgccgggtc accacggtgt ccacccccgc
      541 gccgacgaga gcggccggac gcacgagcgt gatggccagc cctggatggt cggggcggga
      601 ccgctcgagg acgtcctcga ccgcgagcag gtctccgacg agaccctcgt cggaggtggc
      661 ccgccgggtc gagtcctcgg taatcggcag cgggttgtcg ggcgcggctc catacaccat
      721 ggcactggtc acgacgacga ggtgcgacac cccggcagca gccgcggcca tcgagacggt
      781 gagggcggtc cgggtcacct cggcgcgtcg ctcgctcgcg cggcgctcga ggtcgcgctg
      841 caggtcggtg ctgcaggcaa cccacacgac ggtgtcgacc ccggccagga cgctcgcgag
      901 cgccggggag cgcaggtccg cgaggcgcca ctgcacgctc ccggcccgcc cgcgcacagt
      961 gtcgacgccg atcaccgtgc tgaccgggcc cccacggcgc agggcgcgcg ccgtcagcgc
     1021 ggagacgatc tgcccgggga cggacgaggc tgcgcccgcg acagcgacgg cgcggccacg
     1081 gccgcccgcg ggtctggcgg tcgtcgggga actcacatcg gcctcagggt gttgtctagg
     1141 gggtgggtgc cagggacatc atgtccgtag ggcggcgtcc gtgccattgt cacccgtgac
     1201 gaaaccagcc cggtgtcatc acgaagtctc gtccacagtc gcaggaggcg tggaagccat
     1261 gtcagacact ccccaggacc ggggcggcga gggtccgcag gacctgtccg cgatgctgtc
     1321 ccagttcctc ggcgagaccg cccagaaccc cgagctcgcc gaagcgctgc gcgggatggg
     1381 catcgacccc tccgacccgg cgactcttgc gacgctcacc acccagatgc gcagcctctt
     1441 cgacccggcc agtgctccgg acccggccga gctcgccacg acggccgcgc gggcgatcgt
     1501 tgccgccggt gacgccgacc cgtcgctggg tgcagcggcc cggcgcgcgg tggccgacgc
     1561 ggtcggtgtg gcccagctgt gggtcgaccc cgtcacgacc ttgccggcct cccccgggtc
     1621 gggccgggcc tggagc
//
LOCUS       DATAIX010000666         1633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_201865, whole genome shotgun sequence.
ACCESSION   DATAIX010000666 DATAIX010000000
VERSION     DATAIX010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201865"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..1545
                     /locus_tag="VHM65_09795"
     CDS             271..1545
                     /locus_tag="VHM65_09795"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX2706039.1"
                     /translation="MGAFLARLERMDRDVTDSERLDQIGALERVKGAAAAAQARVTAD
                     FDVSRRAAEQAAGVPASRRGRGVGSEVALARRESPARGSRHLGLATALVREMPCTMAA
                     LSTGLITEWAATLICQETAILTRADRQAVDAELGPELSTMSDLQIAKAARAAGYRLDP
                     ASAVRRVRGAVADRRVTCRPAPDCMARVSALLPVAQGVAVFAALDAAAKSARAAGDER
                     SRGQVMADTLVTRVTGQARAQDVPIMVNLVMTQPALLGQDDTPAHLQGFGPVPADLAR
                     DLVTGAQSRSGLRRLFASTDRRRLVAMESPRRTFPRSLADFIALRDQACRTPYCDAPI
                     RHTDHVVPAARGGPTSERNGQGLCERCNYVKALPGWSARADETSGGRHEVVLTTPTGH
                     THTSTAPPPLGHGAIPRDVSPVERRIRLLLAA"
BASE COUNT          229 a          604 c          568 g          232 t
ORIGIN      
        1 tcgccacggg acggcgttcg cgagagtcat ggcgcagtat gacggagggc ctcactcggg
       61 cgagtaggac gtgggcggaa gcaggtcgcc cgcgccttca cttgtcgtgc attgcgttga
      121 gggcgcacat tattggagcg ctgcggtggc acacagccct cttgcagata tcgaacgtgt
      181 gtgcgatact ggagtatgtc gccgatcccg tcttccgctc ccccggggat ggttgacgca
      241 cccaacctgg ggccactcac cgtggcggac gtgggggctt tccttgcccg gctggagcgg
      301 atggaccgcg acgtgacgga ctccgagcgc cttgaccaga tcggcgcgct ggagcgggtc
      361 aagggtgctg ccgccgccgc tcaggcgcgg gtcacggccg acttcgacgt ctcccgacga
      421 gcggcggagc aggccgcggg tgtcccggcg agccgccggg gccgcggcgt ggggtccgag
      481 gtcgcgctcg cgcggcgcga gtcgccggct cgcggctccc gccaccttgg tctggccacc
      541 gcgctggttc gcgagatgcc gtgcacgatg gcggcgttga gcacgggcct catcaccgag
      601 tgggctgcga cactgatctg ccaggagacc gcgatcttga cccgagccga ccgccaggcc
      661 gtcgatgccg agctcggccc ggagctgtcc acgatgagcg acctgcagat cgccaaggct
      721 gcgcgggcgg ccggctaccg gctcgacccg gcgagcgccg tgcgccgggt ccgcggcgcg
      781 gtcgccgatc gccgggtgac ctgccgtcca gcaccggact gtatggcgcg cgtgtcggct
      841 ctgcttccgg tcgcgcaagg agtggccgtc ttcgccgccc tcgacgcggc ggccaagagc
      901 gcacgggccg ctggcgacga gcgctcgcgc ggccaggtca tggccgacac cctggtcacc
      961 cgggtcaccg ggcaagcgcg ggcccaggac gtgccgatca tggtcaacct cgtcatgacg
     1021 cagcccgcac tgctcggaca ggacgacaca cccgcccacc ttcagggctt cgggccggtc
     1081 cctgccgacc tggcccgaga cctcgtcacc ggcgcccagt cacgcagcgg gctccgtcgg
     1141 ctcttcgcca gcaccgatcg gcgccgcctc gtcgctatgg agtcgccccg gcgcaccttc
     1201 ccgaggtcgc tggccgattt catcgcgctc cgcgaccagg cctgccgcac gccatactgc
     1261 gatgcaccca tccgccatac cgaccatgtc gtcccagccg cccgcggcgg gccgaccagc
     1321 gagcgcaacg gccagggcct gtgcgagcgg tgcaactacg tcaaagcgct ccccggctgg
     1381 agtgcccgcg ccgacgagac ctccggggga cgacacgagg tcgtcctcac gacaccgacg
     1441 ggccacaccc acaccagcac cgcgccccca ccgctcggcc acggagcgat cccgcgagac
     1501 gtgagccccg tcgaacggcg cattcgcctc ctcctcgccg cgtaggcgga tcggcctaga
     1561 gaaaggtcga ctcgtcaggc gtgctggtcg gtcacctgct cggcctcatg ggcaggctcc
     1621 ggcgcatcgt ggc
//
LOCUS       DATAIX010000667         2199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_292009, whole genome shotgun sequence.
ACCESSION   DATAIX010000667 DATAIX010000000
VERSION     DATAIX010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292009"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VHM65_09800"
     CDS             complement(<1..512)
                     /locus_tag="VHM65_09800"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEX2706040.1"
                     /translation="MDADWLTRHRPSVLAAAALMPVLVAGLLATIRDEISTAPGVLVL
                     VLVVVAAAATGDRLAGFLAAVSSGVWFDFFLTQPYRTFAVTDPDDVETMVLLLLVCAG
                     VVELALWGRRQQAQGSRRSGYLDGVLSTAEAIAERQPRPQDLIEIVATEITEVLRLDQ
                     CRFLPGKGPTG"
     gene            733..1233
                     /locus_tag="VHM65_09805"
     CDS             733..1233
                     /locus_tag="VHM65_09805"
                     /inference="COORDINATES: protein motif:HMM:NF019913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706041.1"
                     /translation="MSAIDDKWREIPWVGNPKDEGAGSEEMAMPDGRGRARDFDNATI
                     SWTPQTGAHEVHGDIRVKWAQQRWLGYPLTDETGTPDGVGRFSHFEHGSIYWTADLGP
                     HEVHGVIRDFWASQGWERGPAGYPVSDELNDPSAPGGRLSRFQRGVIHWNPATGASFR
                     PGVPFD"
     gene            1298..>2199
                     /locus_tag="VHM65_09810"
     CDS             1298..>2199
                     /locus_tag="VHM65_09810"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2706042.1"
                     /translation="MPASTRPVQPRQDIRFCRASDGVRIAYASHGSGPPMVVATCWLS
                     HLQYDWQSPVWRHFLTDLGRVATVVRYDERGHGLSDRDVDDFSLEARIADLEAVVESS
                     GHERFALMAMSQGGPVAIEYAVRHPERVTRLLFYGSYAAAMRDPTPERVALSDAFEAM
                     IKVGWARPDSAFRRVFTSLMIPHATEEQMRWLDELQRISVSAEVAYAARQQRRRADTT
                     ALVAEIGAPTLVLHSRDDRMNDFEDSRLLASMIPGARLVALESSNHIVLEDEPAWRIF
                     VDEVTAFLDPDRDPNPPAHTPDPAG"
BASE COUNT          355 a          778 c          762 g          304 t
ORIGIN      
        1 ccagtgggtc ctttcccggg aaggaagcga cactggtcga gccgcagcac ctccgtgatc
       61 tcggtcgcga cgatctcgat gagatcctgg gggcgaggct gtcgctcggc gatcgcctcg
      121 gccgtgctga ggaccccgtc gagatagccg gagcgccggc tcccctgcgc ctgctggcgt
      181 cgtccccaca gggcgagctc cacgacgccg gcacacacga ggagcaggag gaccatcgtc
      241 tcgacgtcgt ccgggtcggt gaccgcaaac gtccggtacg gctgagtgag gaagaagtcg
      301 aaccacacac cactggagac agccgcgagg aagccggcga ggcggtctcc cgttgccgcg
      361 gccgcgacga cgacgagcac gaggacaagc acgcccggtg cggtgctgat ctcatcgcgg
      421 atcgtggcga ggagaccggc gacgagaacc ggcatgagcg ccgccgcagc aaggacggag
      481 ggtcggtgcc gggtgagcca gtcagcgtcc atgactccat ggaagcaccc gcggcagccg
      541 atgaaccaaa ccctgacgca atcctgacgg ccacggcgtc cgttcgatgc cggccgcggc
      601 ccagcccgtc caggagtacg gtgtcgtcag ccctgcgggc ggcggcgcca ggggtatcac
      661 ctgggctcgg ctcggcctcc tcaccgcaag ggcccatcgt tcggacgtgg accacgaagc
      721 aaggaggcgg gcatgtcggc aatcgacgac aagtggcgtg agatcccgtg ggtcggcaac
      781 cccaaggacg agggtgccgg cagcgaggag atggcgatgc ccgatggccg gggccgggct
      841 cgtgacttcg acaacgcgac gatttcctgg accccccaga ccggggccca tgaggtgcat
      901 ggagacatcc gggtcaagtg ggcgcagcag cgctggctcg gctatccgct caccgacgag
      961 accggcaccc cggacggggt cgggcgtttc agccacttcg agcacggctc gatctactgg
     1021 actgctgacc tcgggccgca cgaggtccat ggcgtcatcc gcgacttctg ggcctcccag
     1081 ggctgggagc ggggccctgc gggatacccc gtgagcgacg agctcaacga ccccagcgcg
     1141 cccggcggac ggctgagccg cttccagcgc ggggtcatcc actggaaccc cgcgaccggc
     1201 gcgagctttc gccccggcgt gcccttcgac tgatggcccg ccccagatcg tgcggatcga
     1261 ccggggctcg gtgggcaggg ggcgtaacat cctgaggatg ccagcatcga ctcgtccggt
     1321 gcagccccgg caggacatcc gcttctgccg cgcgtccgac ggggtccgca tcgcctacgc
     1381 gagccacggt tccggccccc cgatggtggt cgccacctgc tggctcagcc acctgcagta
     1441 cgactggcag agcccggtat ggcggcactt cctcaccgac ctcggccggg tggcgaccgt
     1501 cgtgcgctac gacgaacgcg ggcacggcct ctcggaccgg gatgtcgacg acttcagcct
     1561 cgaggcccgg atcgccgatc tcgaggcggt ggtcgagagc agcgggcacg agcggttcgc
     1621 cctcatggcc atgtcgcagg gcggtccagt ggccatcgag tatgccgtcc gccaccccga
     1681 gcgggtcacc cgcctgctgt tctacggcag ctacgccgcg gcgatgcgcg atcccacccc
     1741 ggagcgggtc gccctcagcg acgccttcga ggcgatgatc aaggtggggt gggccaggcc
     1801 ggactcggcc ttccggcggg tgttcacctc gctcatgatc ccgcacgcca ccgaggagca
     1861 gatgcgctgg ctcgacgagc tgcagcggat ctccgtctcc gccgaggtcg cctatgccgc
     1921 tcgccagcag cgccggcggg cagataccac cgcactggtc gccgagatcg gcgcaccgac
     1981 cctggtcctg cactccaggg acgatcggat gaacgacttc gaggacagcc gcctgctcgc
     2041 ctcgatgatc ccgggggccc ggctcgtcgc cctggagagc tccaaccaca tcgtcctcga
     2101 ggacgagccg gcctggcgga tcttcgtcga cgaggtgacg gcgttcctcg accccgaccg
     2161 cgatccgaac ccccctgccc acacgccgga tccggccgg
//
LOCUS       DATAIX010000668         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_231887, whole genome shotgun sequence.
ACCESSION   DATAIX010000668 DATAIX010000000
VERSION     DATAIX010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231887"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VHM65_09815"
     CDS             complement(<1..472)
                     /locus_tag="VHM65_09815"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX2706043.1"
                     /translation="MNELLAGLGAVLTRLEPALVELRRDIHAHPELAHHEHRTTALVT
                     ERLAAVGYHIHPLPDTGLVADLGPHEPTYRIALRADLDALPVADQTGLPWASKVDGVC
                     HACGHDLHVAAVLGAGLAFAEFADELRRRDMAIRLVFQPAEEMIPGGAIDVVELG"
     gene            546..1514
                     /locus_tag="VHM65_09820"
     CDS             546..1514
                     /locus_tag="VHM65_09820"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEX2706044.1"
                     /translation="MPTDASAVRPFAVRPSAVRPFAVHPEVASAIAAGGRVVALESTI
                     LAHGLPRGDNRRIADEIEAAVRDAGAVPATIALLDGVVHVGLDPEGLDRLCFGDDILK
                     LSVRDVAVAAALGRTGATTVASTAALAHRAGISVFATGGLGGVHRGASVTFDISADLG
                     VLASCPVVVVCAGVKSILDVAATLEHLETLSVPVLGYRTDAFPGFYLADSGYPAPWRV
                     DSADEVARVVHARRALATDPAGIVVANPLPVDRQVPRELHDRLVAEGLAVLERDGVHG
                     KDVTPRLLEFFHANSGGESLRANVELVLANAALAGEIAVAVAAQSH"
BASE COUNT          195 a          584 c          565 g          212 t
ORIGIN      
        1 agccgagctc gacgacgtcg atggcaccgc cggggatcat ctcctcggcg ggctggaaga
       61 cgaggcggat cgccatatcg cgccggcgca gctcgtcggc aaactcggcg aacgccagcc
      121 cagccccgag caccgcggca acatgcaggt cgtggccgca ggcatgacag accccgtcga
      181 ccttcgacgc ccacggaaga ccggtctggt cggcaaccgg gagcgcgtcc aggtccgcgc
      241 gcaaggcgat ccggtatgtc ggctcgtgcg gcccgaggtc ggcgaccagc cccgtgtcgg
      301 gcaggggatg gatgtggtag cccacggcgg cgaggcgctc ggtcacgagc gcggtcgtgc
      361 ggtgctcgtg gtgggcgagc tcggggtggg cgtggatgtc gcggcgcagc tcgaccagag
      421 ccggttcgag gcgggtgagg acggccccta ggccggcaag cagctcattc attgtgcgca
      481 ccagggtatc ggcccggcgc agccgctgac cccggcgcac gcgggacccc agtacgctgc
      541 tcgccgtgcc cactgatgcc agcgccgtcc ggcccttcgc cgtccggccc agcgccgtcc
      601 ggcccttcgc cgtccacccc gaggtcgcct cggccatcgc cgcgggaggg cgggtggtcg
      661 cgctggagag cacgatcctc gcccacggcc tgccacgcgg cgacaaccgc cggatcgccg
      721 acgagatcga ggcggccgtt cgcgacgcgg gcgccgtccc ggcgacgatc gccctgctcg
      781 acggggttgt gcatgtcggc ctcgacccgg aggggctcga ccggctctgc ttcggcgacg
      841 acatcctcaa gctgtcggtc cgcgacgtcg cggtcgcggc cgcactgggg cgcacgggtg
      901 cgacgacggt ggcctcgacg gcggccctgg cgcaccgcgc ggggatctcg gtgtttgcca
      961 ccggcggact cgggggcgtg caccgaggag cctcggtgac cttcgacatc tcggccgacc
     1021 tcggggtcct cgcctcgtgc ccggtcgtcg tcgtctgcgc cggggtcaag tcgatcctcg
     1081 acgtggcggc gacgctggag catctcgaga cgctgtccgt ccccgtcctg ggctaccgca
     1141 ccgacgcctt ccccgggttc tacctggccg actcgggcta tccggcgccg tggcgggtcg
     1201 actcggccga cgaggtcgcg cgagtcgtcc acgcccgccg cgcactggcc accgacccgg
     1261 ccggcatcgt cgtcgccaac ccgctgcccg tcgaccggca ggtgccgcgc gagctgcacg
     1321 accggctcgt cgccgagggc ctcgccgtcc tcgagcgcga cggcgtccac ggcaaggacg
     1381 tcacgccacg gctgctcgag ttcttccacg cgaactccgg cggcgagagc ctgcgcgcca
     1441 atgtcgagct cgtcctggcc aacgccgcgc tcgccggcga gatcgcggtc gccgtcgcgg
     1501 cccagagcca ctgaccggta tgacgtcgcc accgcgggcg cgccccgtcg tcgtcg
//
LOCUS       DATAIX010000669         2323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_202025, whole genome shotgun sequence.
ACCESSION   DATAIX010000669 DATAIX010000000
VERSION     DATAIX010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202025"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /gene="pyrH"
                     /locus_tag="VHM65_09825"
     CDS             <1..699
                     /gene="pyrH"
                     /locus_tag="VHM65_09825"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX2706045.1"
                     /translation="RVLLKLSGEVFGGGRVGVDPAVVKGIARQIAEAVRDGIEVAIVV
                     GGGNFFRGAELQQKGMDRSRADYMGMLGTVMNCLALQDFLDKAGVETRVQTAITMGQV
                     AEPYIPRRAIRHLEKGRVVIFGAGMGMPFFSTDTVSAQRALETHCDRLLIAKSGVDGI
                     YTADPKTDPTATKIEALTFDEALSRGLRILDAAAFALCQENKLPMLVFGMEGEGDITR
                     ALRGEKIGTLVTAA"
     gene            771..1328
                     /gene="frr"
                     /locus_tag="VHM65_09830"
     CDS             771..1328
                     /gene="frr"
                     /locus_tag="VHM65_09830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX2706046.1"
                     /translation="MIDETLFEAEEKMEKAVEVAKEDFAGIRTGRANPGMFNKLLVDY
                     YGSPTPLQQLASFSTPEARTILIKAFDASTMHAIEKTIRDSDLGMNPSNDGVVIRCIL
                     PILTEERRRDYIKLAHHKAEEARVSIRNVRRRAKSDLEHLVKEGQVGEDDGSRGEKEL
                     DQLTKKYVDTIDTLLRGKEAELLEV"
     gene            1354..2223
                     /locus_tag="VHM65_09835"
     CDS             1354..2223
                     /locus_tag="VHM65_09835"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX2706047.1"
                     /translation="MTDATAADPAQGQAAPAPSRAGRNLPAAIGSGLVLGALVVATLF
                     VRKEGFLVLAIVSVSIGVWELVTAFGKGGIHVPLVPSVVGTVSMMTAAYVGGPQALAV
                     TFALTCVAVLVWRRADGVQGAARDVSGGMLVAAYPSMLAGFSALLLAPADGSWRGFVF
                     VLVTVCSDVGGYAVGVTFGKHPMAPSISPKKSWEGFAGSVLLCVASGIVSVVLIFHRQ
                     WWVGLLLGLAVAVSATIGDLVESLIKRDLGIKDMSDVIPGHGGLMDRLDSLLVTAPVV
                     WAILTVFAPLPGG"
BASE COUNT          359 a          811 c          789 g          364 t
ORIGIN      
        1 cgggttctgc tcaagctctc gggagaggtc ttcggcggcg ggcgcgtcgg cgtcgacccg
       61 gccgtcgtca aggggatcgc caggcagatc gccgaggcgg tgcgggacgg gatcgaggtg
      121 gccatcgtcg tcggcggcgg aaacttcttc cgtggggcgg agctgcagca gaagggcatg
      181 gaccgctccc gggccgacta catgggcatg ctcggcacgg tgatgaactg cctcgcgctg
      241 caggacttcc tcgacaaggc gggcgtcgag acccgcgtcc agaccgccat cacgatgggc
      301 caggttgccg agccctacat cccccggcgt gcgattcgtc acctcgagaa gggccgggtc
      361 gtcatcttcg gagccggcat gggtatgccg ttcttctcca cggacacggt gagcgcccag
      421 cgtgcgctgg agacccactg cgaccggctg ctcatcgcca agagcggcgt ggacgggatc
      481 tacacggccg acccgaagac cgacccgacc gcgaccaaga tcgaggccct gaccttcgac
      541 gaggcgctgt cccgggggct gcgcatcctc gatgccgcgg ccttcgcgct gtgccaggag
      601 aacaagctgc cgatgctcgt cttcgggatg gagggcgagg gagatatcac ccgggccctt
      661 cgaggtgaga agatcggcac gctggtcacc gcagcgtgac cggtcgaccc gacgggcacg
      721 agccgcccag ccgcaccacc cgcgacctga ccttgaggag cacgacggcg atgatcgatg
      781 agaccctctt cgaggccgag gagaagatgg agaaggccgt cgaggttgcc aaggaggact
      841 ttgcgggcat ccgcaccggc cgggccaacc cgggcatgtt caacaagctc ctcgtcgact
      901 actacgggtc gccgacgccg ctgcagcagc tcgcctcctt ctcgacgccc gaggcgcgca
      961 cgatcctcat caaggcgttc gacgcctcga cgatgcacgc gatcgagaag acgatccggg
     1021 actccgacct cggcatgaac ccgagcaacg acggtgtcgt catccggtgc atcctgccga
     1081 tcctgaccga ggagcgccgc cgcgactaca tcaagctggc gcaccacaag gccgaagaag
     1141 cccgggtctc gatccgcaat gtccggcgca gggccaagtc cgacctcgag cacctcgtca
     1201 aggagggcca ggtcggcgag gacgacggct cccgcggcga gaaggagctc gaccagctga
     1261 cgaagaagta cgtcgacacc atcgacaccc tgctcagggg caaggaagcc gagctgctcg
     1321 aagtctgagc cgcacccgga ccgcgagcac ccgatgaccg acgccaccgc tgcagacccc
     1381 gcccagggcc aggccgcgcc cgccccttcc cgggccgggc gcaacctgcc cgccgctatc
     1441 ggcagcggac ttgtgctcgg cgctctggtc gtcgcgacgc tgttcgtccg caaggaggga
     1501 ttcctcgtcc tggcgatcgt gtcggtctcc atcggggtct gggagctcgt cacggcgttc
     1561 ggcaagggtg ggatccacgt ccccctcgtc ccgagcgtcg tcggcaccgt ctcgatgatg
     1621 actgcggcgt atgtcggggg tccccaggcg ctggccgtca ccttcgcttt gacgtgtgtc
     1681 gcagtgcttg tctggcgccg tgccgacggg gtccagggag cggcgcgcga cgtctcgggc
     1741 gggatgctcg tcgccgccta cccctcgatg ctcgccgggt tctccgcgtt gctgctggct
     1801 ccggccgacg gttcctggcg ggggttcgtc ttcgtcctgg tcaccgtctg cagcgacgtg
     1861 ggggggtatg ccgtcggcgt gaccttcggc aagcacccca tggccccgtc gatcagcccc
     1921 aagaagtcgt gggaaggctt cgccgggtcg gttctcctgt gtgtcgcgag cggcatcgtg
     1981 tccgtcgtgc tcatcttcca ccggcagtgg tgggtcgggc tcctgctcgg cctggccgtc
     2041 gccgtctcgg cgacgatcgg ggacctcgtc gagtcgctca tcaagcgcga cctcggcatc
     2101 aaggacatgt ccgacgtcat accgggtcac ggcggcctga tggaccggct cgactcgctg
     2161 ctcgtcaccg ccccggtcgt ctgggccatc ctcacggtct tcgccccgct ccccgggggc
     2221 tgagctccga gggacccgga ccctgcccac ggctgcgaca atgggggcaa tgccaaccga
     2281 ccagcccgtg gacccccgcc ccgcggcgcg ggccgtcata ccc
//
LOCUS       DATAIX010000670         4068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_138076, whole genome shotgun sequence.
ACCESSION   DATAIX010000670 DATAIX010000000
VERSION     DATAIX010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138076"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VHM65_09840"
     CDS             complement(<1..621)
                     /locus_tag="VHM65_09840"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2706048.1"
                     /translation="MDLLVLGGTAYLGRYVTQAALARRHRVTCVARGSVRLPPEGAEL
                     VRADRDRDGALAPVAGRPWDSVIDVARHPGQVRRAVAGLVATTAHYVFVSTGNVYADH
                     RRPGLDESAPTLPPLAGEVMSDMSEYGAAKVACEDHVVAGFGPGHSLIARSGLIGGPD
                     DWSGRSGYWPWRFAHPSNPEGRVLVPDTPDLSTQVIDVRDLAQWLVQ"
     gene            764..1138
                     /locus_tag="VHM65_09845"
     CDS             764..1138
                     /locus_tag="VHM65_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706049.1"
                     /translation="MFALALTNVALVLGWVISGYQRVSEELTRERRTAYADLLCAVQS
                     ARRGERADANIADLAGRARLRSSPQMHASRILDDFVAAIHGDEATWERERSRFIEAAR
                     YESLVNSATRRWLRRSSVYAPR"
     gene            1180..1599
                     /locus_tag="VHM65_09850"
     CDS             1180..1599
                     /locus_tag="VHM65_09850"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2706050.1"
                     /translation="MRGRSESDGTVDRWSRHDWWGVVLEAPDARALAHFYADLLGWAV
                     ASESDTDATIGPADGVAYLAFQTSSSYVRPVWPVVPGTQQMTMHLDFEVDDLEPAVAH
                     AVSLGGELAAFQPQDNVRVVLDPAGHPFCLYTPGDSP"
     gene            1596..2093
                     /locus_tag="VHM65_09855"
     CDS             1596..2093
                     /locus_tag="VHM65_09855"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX2706051.1"
                     /translation="MTEGVPPRSESVLNDPERVLLEAFLDDNREELVATLAGLTDEQA
                     RRRLVPSLTTPLGLVKHAAFVERAWFQAALAGRTRAEIGIPDTVDESFALTDADTVDS
                     VVTDFRRACGESREAAARYALDDRALHNRRGPLSLRWVYVHMIEELARHAGHADILRE
                     QIRPV"
     gene            2151..3926
                     /locus_tag="VHM65_09860"
     CDS             2151..3926
                     /locus_tag="VHM65_09860"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX2706052.1"
                     /translation="MAREFTKVGVIGFGTMGAGIVEVLARNGIDVVAIDVDDGALARG
                     KGKLVGSTGRAVARGKLSEADQAALIGRVTLATDLAGLAGCQLVIEAVPEKLALKKSL
                     LAELDTIVADDAILATNTSSLPVTEIAVATKLPTRVIGMHFFNPAPIMQFVEVIRTVV
                     TAEDVFADAKALAERLGKMPVVVGDKAGFIANALLFGYLNHAVTMYEQKYATREDVDA
                     SMRFGCGLPMGPLALMDLIGLDTAYEILDTMYHQGRDRLHAPTPIIKQMVNAGLMGRK
                     TGRGFYTYAAPGSSEVVADALTPTPAGEGDLALRPIAKVGVVGSGTMATGIVEVFAKA
                     GYDVTYVARGADKVAGVRTAIDKSCARAIGKGRMTQEAADALLARLTGATERSALADV
                     DLVVEAIAEDLAIKQELFRDLDAICKPGAILATTTSSLPIISCANVTKRVEDVIGMHF
                     FNPAQVMKLVEVVHTVATSADVIDTTVDLTRKIGKHPVQCADRSGFIVNALLFPYLND
                     AVRMLEAHYATADDIDTAMKTGCGYPMGPFELLDVVGLDVSLAIQRELYLEFREPGFA
                     PAPLLEHLVTAGYLGRKSKRGFRVY"
BASE COUNT          613 a         1483 c         1362 g          610 t
ORIGIN      
        1 ctgcacgagc cactgcgcca ggtctcggac gtcgatgacc tgggtgctca ggtccggcgt
       61 gtcgggcacg agcacgcggc cctcggggtt gctcggatgc gcaaaccgcc acggccagta
      121 gccgcttcgt ccgctccagt catccgggcc gccgatcaga ccggacctcg cgatgagcga
      181 atgcccgggg ccgaagcccg ccacgacgtg gtcctcgcag gcgaccttgg cggcgccata
      241 ctcgctcatg tcgctcatga cctccccggc cagcggtggc agggtcggtg cgctctcgtc
      301 caggccgggt ctgcggtggt cggcgtagac gttgccagtc gagacgaaca cgtagtgggc
      361 tgtggtggcg acgaggccgg ccaccgcgcg gcgcacctga ccagggtggc gggccacatc
      421 gatgaccgag tcccagggcc ggccagcgac cggtgcgagg gcgccgtccc ggtcccggtc
      481 cgcccgcacg agctctgcgc cctcgggtgg gagccgcacg ctcccacgag ccacgcacgt
      541 gacgcgatgc ctgcgggcca gcgcagcctg ggtgacgtag cggccgaggt atgccgtccc
      601 gcccagtacc aagaggtcca tggcgccagg ccagcacggc gccgctcctg gcgacaaggg
      661 gtttcgccct gggcggagag cagctccgcg gccgggcgtt gtgtcgcagc ccggccccgg
      721 gccgagcatg atggggtcat ggcgctagcg gtgaggatca cggttgtttg ctctcgcact
      781 gaccaacgtc gcgctcgtcc tcggatgggt gatctcgggc taccagcggg tgtccgagga
      841 gctcacccga gagcgccgca ccgcgtatgc cgacctgctc tgcgcggtgc agtcggcgcg
      901 gcgaggggag cgggctgacg cgaacatcgc cgacctggcc gggcgcgcgc ggttgcgcag
      961 ctccccccag atgcacgcgt cccgcatcct cgacgacttc gtcgcagcca tccacggcga
     1021 cgaggccacg tgggagaggg agcgcagccg cttcatcgag gcggcccgct acgagtcgct
     1081 cgtcaactcg gcgacccggc gctggctccg ccgaagctcg gtctacgccc cgaggtagct
     1141 ctgcttgggt gccgaccggt gctctggcac agtagtggga tgcgcggacg ctcggagtcg
     1201 gacgggacgg ttgaccgctg gtcgcgccat gactggtggg gagtcgtgct cgaagcaccc
     1261 gatgcgcggg cgctcgcgca cttctacgcg gacctgctcg gctgggcggt cgcttcggag
     1321 agcgatacgg atgccacgat cgggcccgcc gacggggtcg cctacctcgc cttccagacg
     1381 tcatcgtcct acgtccggcc ggtgtggccg gtggtgcccg gcacccagca gatgacgatg
     1441 cacctcgact tcgaggtcga cgacctggag cccgcggtgg cccacgccgt ctcgctcggc
     1501 ggtgagctcg ccgctttcca gccgcaggac aacgtccgcg tggtcctgga ccccgccggg
     1561 caccccttct gcctctacac ccccggggac tccccgtgac cgagggcgtg ccaccccgga
     1621 gcgagagcgt gctcaacgat cccgaacgcg tgctgctcga ggcgttcctc gacgacaacc
     1681 gggaggagct cgtggccacg ctggcgggac tcaccgacga gcaggcccgt cgccggctgg
     1741 tcccgtccct gacgaccccg ctcggcctgg tcaagcacgc cgcctttgtc gagcgggcct
     1801 ggtttcaggc ggcgctggcg gggcggacgc gcgccgagat cggcatcccc gacacggtcg
     1861 acgagagctt cgcgctcacc gacgccgaca ccgtcgactc ggtcgtgacg gacttccgcc
     1921 gggcctgcgg cgagtcacgc gaggcggccg cccgctacgc cctcgacgac cgggcgctgc
     1981 acaaccgacg tgggccgctg agcctgcgct gggtctacgt ccacatgatc gaggagctgg
     2041 cccgccacgc tggccatgcc gacatcctgc gggagcagat ccgcccagtg tgaccactct
     2101 cgccccgccc gccggtgcac ccgtcatact ggctctgcca gactcaccct atggccagag
     2161 agttcacgaa ggttggcgtc atcgggttcg gcaccatggg tgcgggcatt gtcgaggtcc
     2221 ttgcccgtaa cggcatcgac gtggtcgcga tcgacgtcga tgacggagcc ctcgctcgcg
     2281 ggaagggtaa gctcgtgggc tccacggggc gcgccgtcgc gcgcggcaag ctcagcgaag
     2341 ccgaccaggc cgccctgatc gggcgagtca ccctcgccac cgacctggcc gggctcgcgg
     2401 gctgccagct cgtcatcgag gccgtcccgg agaagctcgc cctcaagaag agcctcctgg
     2461 ccgagctcga cacgatcgtc gccgacgacg cgatcctggc caccaacacg tcctccctcc
     2521 cggtcaccga gatcgccgtt gcgaccaagc tgccgacccg ggtcatcggc atgcacttct
     2581 tcaacccggc accgatcatg cagttcgtcg aggtcatccg caccgtggtg actgcagagg
     2641 acgtcttcgc cgacgccaag gctctcgccg agcggctggg caagatgccc gtcgtcgtcg
     2701 gtgacaaggc cggcttcatc gccaacgcgc tgctcttcgg ctacctcaac cacgcggtga
     2761 cgatgtacga gcagaagtac gccacgcgcg aggacgtcga tgcctcaatg cggttcggct
     2821 gtggcctgcc tatgggcccg ttggcgctga tggacctcat cggcctggac acggcctacg
     2881 agatcctcga cacgatgtac caccagggtc gcgaccgcct gcacgcaccg acccccatca
     2941 tcaagcagat ggtcaacgcc ggactcatgg gccgcaagac cggacgcggc ttctacacct
     3001 acgccgcacc ggggtcctcc gaggtcgtcg ccgacgctct cacccccacg cccgcgggcg
     3061 agggcgacct cgcgctgcgg ccgatcgcca aggtcggcgt cgtcggttcg ggcacgatgg
     3121 caaccgggat cgtcgaggtc ttcgccaagg ccgggtatga cgtcacctac gtcgcgcggg
     3181 gggccgacaa ggtcgccggg gtcaggaccg ccatcgacaa gagctgcgca cgggccatcg
     3241 gcaagggccg gatgacgcag gaggctgctg atgccctcct cgcccggctc accggcgcca
     3301 ccgagcgctc ggctctggcc gatgtcgacc tcgtcgtcga ggccatcgcc gaggacctcg
     3361 cgatcaagca ggagctgttc cgcgacctcg acgcgatctg caagccgggc gccatcctcg
     3421 cgacgacgac gagctcgctg ccgatcatct cgtgcgccaa cgtgaccaag cgcgtcgaag
     3481 acgtcatcgg aatgcacttc ttcaaccccg cccaggtcat gaagctcgtc gaggtcgtcc
     3541 acacggtcgc cacctcggcc gacgtcatcg acacgaccgt cgacctgacc cgcaagatcg
     3601 gcaagcaccc cgtccagtgc gccgaccgtt ccggcttcat cgtcaacgcg ctcctcttcc
     3661 cctatctcaa cgacgcggtg cggatgctcg aggcgcacta cgccaccgcc gacgacatcg
     3721 acacggcgat gaagaccgga tgtggctacc cgatgggccc gttcgagctg ctcgacgtcg
     3781 tcgggctcga tgtctccctg gcgatccaac gcgagctcta cctcgagttc cgcgagcccg
     3841 ggttcgcccc cgcgccgctg ctcgagcacc tcgtcaccgc gggctacctc ggccgcaaga
     3901 gcaagcgcgg cttccgggtc tactgaccgc gacgggcgca ggcccggcat acgggaggtc
     3961 cggcatacgc gccattctcc cgtatgccgg acctgcgtgt gccggacctc ccgtctgccg
     4021 gacctgcgtg tgccggacct gcgtgtgccg gaaccgcgta tgccggaa
//
LOCUS       DATAIX010000671         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_232529, whole genome shotgun sequence.
ACCESSION   DATAIX010000671 DATAIX010000000
VERSION     DATAIX010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232529"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VHM65_09865"
     CDS             <1..796
                     /locus_tag="VHM65_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2706053.1"
                     /translation="IWALFPIMFLLSAALNPSGQLATSSLLPSDFSLVNLQSLSQDAV
                     RPYWGWYKNSLVICTVGALLSMFLGACAAFAFSRLRFTGRRMGLLSLLLLQLFPAILA
                     LVAMYITFAKVGDVVPAFGLNTTWGLILAYMGGAMGSNVWLLKGYFDTIPKELDEAAI
                     VDGASHARIFFTMTLRLVAPILVTVFMLSFVGLFGEFMLASVFLADVEKQTLGVGLYG
                     LSNGRDANERFGFFAAGSLLCSLPVMLLYLAFQKQLVGGLTQGSVK"
     gene            793..>2445
                     /locus_tag="VHM65_09870"
     CDS             793..>2445
                     /locus_tag="VHM65_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HEX2706054.1"
                     /translation="MSTRSSGGSVNLLAGPHHDGSALYVSNLAPALGEVVRVRLRVPH
                     DHAPSAVHVRLVVDGEQKFVEARVEHADEIETWWIADLTCHNPVTGYRFLLDGGRAGY
                     AWLNGAGLHRRDVPDASDFRLVAHSDPPPRWARDAVVYQIFPDRFARSAAADSRAAPG
                     WALPAPWDEPVDGRMGPASRQLYGGDLDGVGERLDYLVDLGVTVIYLTPFFPAHSNHR
                     YDATTFAAVDPLLGGDEALRRLTDAAHARGIRVMGDFTTNHTGSAHEWFLAARADPRA
                     PERDFYFWEGQEYVAWLGVKSLPKLNYDSVTLRERLFDDPQGVVRKWLGPDGGLDGWR
                     VDVANMTGRYGAQDHNHEVAALMRDAMVSHAPGSLLVGEHVHDFSVDVPGTGWHGVMN
                     YAGFTKPVWTWLSDPVTAPDFLGSPVIVPRLGGVETVETIRDFTSRVSWQAVTHSFNL
                     VGSHDTTRIRTLVGADPRLVDVAAGLLLTMPGIPMITYGDEIGMAGEWGEDGRRPMPW
                     EPQDWDARLREVYRSLIGLRRSSAALRHGGLRWVYADHDAIVY"
BASE COUNT          319 a          881 c          822 g          423 t
ORIGIN      
        1 catctgggcg ttgttcccga tcatgttcct gctctccgcg gcgctgaacc cctcgggcca
       61 gctggccacg tcctcgctgc tgcccagcga cttctcgctg gtcaacctgc agtcgctgag
      121 ccaggacgcc gtgcggccct actggggctg gtacaagaac tcgctggtca tctgcaccgt
      181 cggcgcgctc ctgtcgatgt tcctcggtgc ctgcgccgcc ttcgccttct cccggctcag
      241 gttcacgggc cggcgcatgg gtctgctgtc gctgctgctg ctccagctct tccccgccat
      301 cctcgcgctg gtcgccatgt acatcacctt cgccaaggtc ggcgatgtcg tccccgcctt
      361 cgggctcaac acgacctggg gcctgatcct ggcctacatg gggggagcga tgggctcgaa
      421 cgtctggctg ctcaagggct acttcgacac gatccccaag gagctggacg aggcggcgat
      481 cgtcgacggg gcgagccacg cccgaatctt cttcacgatg acgctgcgcc tcgtggcgcc
      541 gatcctcgtc acggtgttca tgctctcctt cgtcggcctg ttcggcgagt tcatgctggc
      601 cagcgtcttc ctcgccgatg tcgaaaagca gaccctcggg gtgggcctgt acggcctgtc
      661 caatggccgg gatgccaatg agcggttcgg cttcttcgcc gcggggtcgc tgctgtgctc
      721 gctgccggtc atgctgctct acctggcctt ccagaagcag ctcgtcggtg ggctcaccca
      781 gggttccgtg aagtgagcac ccgctcgtcg ggggggtcgg tcaacctgct ggccggcccc
      841 caccacgacg ggtcggcgct ctacgtctcg aacctggctc cggccctcgg ggaggtcgtc
      901 cgggtcaggc tgcgggtccc tcacgaccac gccccctcgg cggtccacgt ccgcctcgtc
      961 gtcgacggcg aacagaagtt cgtcgaggcc agggtcgagc acgcggacga gatcgagacg
     1021 tggtggatcg ccgacctcac gtgtcacaac ccggtcaccg gctaccgctt cctgctcgac
     1081 gggggccgcg cgggctacgc ctggctcaac ggtgccggcc tgcaccggcg ggacgtcccg
     1141 gatgccagcg acttccgcct ggtggcccac tcggaccccc cgccgcggtg ggcccgtgac
     1201 gcggtcgtct accagatctt cccggaccgc ttcgcccggt cggctgcggc cgactcgcga
     1261 gcggcgccgg gctgggcgct gccggcgcct tgggacgaac cggtcgatgg gcggatggga
     1321 ccggccagcc gccagctcta cggcggcgac ctcgatggag tcggggaacg gctcgactac
     1381 ctcgtcgatc tcggcgtcac cgtcatctac ctcaccccgt tcttcccggc ccactccaac
     1441 catcggtacg acgccacgac cttcgcggca gtcgacccgc tcctcggtgg ggacgaggcg
     1501 ctgcgccgcc tcaccgacgc ggcccacgcc cgcggcatcc gggtcatggg ggacttcacg
     1561 accaaccaca ccgggtccgc gcacgagtgg ttcctcgcgg cccgggccga cccgcgtgcc
     1621 cccgagcggg acttctactt ctgggagggg caggagtacg tcgcctggct cggggtcaag
     1681 tcgctgccca agctcaacta cgactcggtg accttgcgcg agcggctctt cgacgacccg
     1741 cagggcgttg tgcgcaaatg gctcggcccg gacggtgggc tcgacgggtg gcgcgtcgac
     1801 gtggcgaaca tgaccggccg gtatggcgcg caggaccaca accacgaggt cgcggcgctc
     1861 atgcgcgacg cgatggtcag ccacgcaccg ggctcgttgc tcgtgggtga gcacgtccac
     1921 gacttcagcg tcgacgtgcc cggcaccggc tggcacggcg tcatgaacta tgccggcttc
     1981 accaagccgg tctggacctg gctcagcgac ccggtgacgg cgccggactt cctcgggtcg
     2041 ccggtcatcg tgccccgact cggtggtgtc gagacggtcg agacgatccg tgacttcacc
     2101 tcgcgggtgt cctggcaggc ggtcacccac tcgttcaacc tcgtcgggtc ccacgacacg
     2161 acgcggatcc gcacgctcgt cggggcggac ccccgcctcg tcgacgtggc tgccgggctg
     2221 ctgctcacga tgccggggat accgatgatc acctacggcg acgagatcgg tatggcgggt
     2281 gagtggggtg aggacgggcg gcggccgatg ccctgggagc cgcaggactg ggacgctcgc
     2341 ctgcgcgagg tctaccggtc gctcatcggg ctgcgccgca gctcggcggc gctgcgccac
     2401 ggcggcctgc gctgggtcta tgccgaccac gacgccatcg tctac
//
LOCUS       DATAIX010000672         4869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_232639, whole genome shotgun sequence.
ACCESSION   DATAIX010000672 DATAIX010000000
VERSION     DATAIX010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232639"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VHM65_09875"
     CDS             <1..942
                     /locus_tag="VHM65_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706055.1"
                     /translation="YLGQGAQAGKVSDLAYHNSLMVQIWSMLATGETSLAVRALRALP
                     PTPSTGTWVCYTRCHDDIGWAIDDGDAAAVGLGGWAHRSFLADWYSGAFPGSPARGLV
                     FQANEQTGDRRISGTAASLCGLESARTTADVDTALARIQLAHAIVAGWGGIPVLWSGD
                     ELGLTNDPGWAAEPGHEDDNRWAHRPRLDPARAALRDDLTTVEARVFHGLAHLARVRA
                     GLPHLHASASAEVLEGADPGLLVVARRHAAGVMVGVYNVTRSPRPPPVGRLRRLGITD
                     PYDALSGHRPQWTGDGQIWVGPYGASWFVEAGSPHAS"
     gene            complement(976..3558)
                     /gene="clpB"
                     /locus_tag="VHM65_09880"
     CDS             complement(976..3558)
                     /gene="clpB"
                     /locus_tag="VHM65_09880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX2706056.1"
                     /translation="MDLHLTTKASEALSAAVRDAAASGHAQTEPAHLLKALAEQAETT
                     TPALLTAAGTSARALAQAAAKTLSGMPSATGSSMATPGLSRGSLAALENARAAMSAMG
                     DTYVSTDHLVLGLARSRQFGLDPAAIEAAIPALRGGAKVTTENPEATFDALGKYGTDL
                     TAQAREGKLDPVIGRDAEIRRVVQVLSRRTKNNPVLIGEPGVGKTAVVEGLAQRMVAG
                     DVPESLRGKTLIALDLGAMVAGAKYRGEFEERLKAVLEEIKSSNGQVVTFIDELHTVV
                     GAGATGEGSMDAGNMLKPLLARGELRMVGATTLDEYRKHIEKDAALERRFQQIFVGEP
                     SVEDTIGILRGLKERYEAHHKVTIADSALVAAATLSDRYISGRQLPDKAIDLVDEAAS
                     RLRMEIDSSPVEIDELRRAVDRLKMEELALEKESDEASRARLSRLRKDLADRSEALAA
                     LTARWEAEKAGLNRVGELKASIDEARIHAERLQREGNLAEASALLYGQIPALERDLAA
                     AQAAEAGMPGAGVPGGTPVAPMVKEEVGADDIADVISAWTGIPAGRLLEGETDKLLRM
                     EDFLGRRLIGQSEAVRAVSDAVRRSRAGIADPDRPTGSFLFLGPTGVGKTELAKSLAD
                     FLFDDERAMVRIDMSEYSERHAVARLIGAPPGYVGYEEGGQLTEAVRRRPYSVVLLDE
                     VEKAHPETFDILLQVLDDGRLTDGHGRTVDFRNVILVMTSNLGSQYLIDPVMEPAAKR
                     EAVLGMVRMAFKPEFLNRLDEVVIFEALSTAELAAIVGLTVAGLGARLAERRITLDVT
                     PAAREWLAVTGFDPAYGARPLRRLVQREIGDRLAKALLSGAVRDGQTVTVDRDPDEDG
                     LILH"
     gene            complement(3727..4482)
                     /locus_tag="VHM65_09885"
     CDS             complement(3727..4482)
                     /locus_tag="VHM65_09885"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX2706057.1"
                     /translation="MRTVVIGAVAAGLAAALVSAPATAAVPSAAVPSAIVLSYPFENL
                     AGGIVRDVSPSRLDGMLVNTTAAAATSASVPGHGTAVALVGAGHQFVAVPEGNALDVD
                     RYTLTALVRYTGIPNDQTLGRWEVLEKAGAYWMNVRTNGRVRVGGFYGSCTATSAWKF
                     FDSTTAVPLNTWTHLAATYNGSTLTVWINGSKSGSMAVTGRTCSNNEPLAIGAKNAPS
                     KGLLEAFWDGQLDDVRIYNRALSAAGIAALVPT"
BASE COUNT          666 a         1755 c         1727 g          721 t
ORIGIN      
        1 tatctgggcc agggggccca ggccggcaag gtgagcgacc tcgcctacca caacagcctc
       61 atggtccaga tctggtcgat gctggccacc ggcgagacct cgctcgccgt ccgcgcgctg
      121 cgtgcgctgc ccccgactcc gagcaccggc acgtgggtct gctacacccg gtgccacgac
      181 gacatcggct gggcgatcga tgacggcgac gccgcagcgg tcgggctcgg gggctgggcg
      241 caccgcagct tcctggccga ctggtactcc ggcgccttcc ccggctcacc cgcgcgcggg
      301 ctcgtcttcc aggccaacga gcagaccggc gaccgccgga tcagtggcac cgccgcctcg
      361 ctgtgcgggc tggagtcggc gcggacaacc gccgacgtcg acacggcctt ggcccggatc
      421 cagctcgccc acgcgatcgt tgccgggtgg ggcggcatcc ctgtcctgtg gtccggcgat
      481 gagctcggcc tcaccaacga ccccggctgg gcggccgagc ccgggcatga ggacgacaac
      541 cgatgggcgc accgcccccg cctggacccc gcgcgggctg ccctccggga cgatctcacg
      601 accgtcgagg ccagggtctt ccacggtctg gcgcacctcg cccgggtgcg ggcgggcctg
      661 ccccacctgc acgcgagcgc gagcgccgag gtcctcgagg gcgccgaccc gggccttctc
      721 gtcgtggccc gccggcacgc ggccggcgtc atggtcgggg tctacaacgt cacccggagc
      781 ccgcgcccgc cgcctgtcgg ccggctgcgc cggctcggca tcaccgaccc gtatgacgcc
      841 ctgtcgggtc accgtccgca gtggaccggg gacggccaga tctgggtcgg cccgtatggc
      901 gcgtcctggt tcgtcgaggc cggctcaccc cacgcctcct gagccgaggt caccatcctg
      961 caggcctgca ccgggtcagt gcaggatgag gccgtcctcg tcggggtcgc ggtcgacggt
     1021 cacggtctgg ccgtcacgga ccgctccaga caggagcgcc ttggcgagcc ggtccccaat
     1081 ttcccgctgg accagacggc gcagcgggcg cgcgccatac gccgggtcga agccggtgac
     1141 cgcaagccac tcgcgtgcgg cgggcgtgac gtcgagggtg atccggcgct cggcgaggcg
     1201 cgcgccgagg ccggcgacgg tgagcccgac gatcgccgcg agctcggccg tcgagagcgc
     1261 ctcgaagatg acgacctcgt cgagccggtt gaggaactcg ggcttgaacg ccatccggac
     1321 cattcccagg accgcctcac gcttggccgc gggctccatg accgggtcga tgaggtactg
     1381 ggacccgagg ttcgaggtca tgacgagaat gacgttgcgg aagtcgaccg tgcggccgtg
     1441 cccgtcggtg agccgtccgt cgtcgaggac ctgcaggagg atgtcgaacg tctccgggtg
     1501 cgccttctcg acctcgtcga ggagcacgac ggagtagggc cgccgacgga cggcctcggt
     1561 gagctggccg ccctcctcgt agccgacata cccggggggc gcaccgatga ggcgggcgac
     1621 cgcgtggcgc tcggagtact ccgacatgtc gatccggacc atggcccgct cgtcgtcgaa
     1681 gaggaagtcc gcgagcgact tggcgagctc ggtcttgccg acgcccgtcg ggccgaggaa
     1741 gaggaacgac ccggtcggcc ggtcggggtc ggcgatcccg gcccgggagc gtcgcaccgc
     1801 gtcggacacg gcccgcacgg cctccgactg gccgatgagg cggcgcccga ggaagtcctc
     1861 catccggagc agcttgtcgg tctcgccttc caggagccgc ccggccggga tccccgtcca
     1921 ggccgagatg acgtcggcga tgtcgtcggc gcccacctcc tccttgacca tcggcgccac
     1981 gggggtccct cccgggactc ccgcgcccgg cataccggct tcggccgcct gggccgccgc
     2041 aaggtcacgt tccagcgcgg gaatctggcc gtagagcagg gccgaggcct cggcgaggtt
     2101 gccctcgcgc tggagccgtt cggcgtgtat ccgcgcctcg tcgatcgagg ccttgagctc
     2161 gccaacccgg ttgagcccgg ccttctcggc ctcccagcgg gcggtcagtg cagccagcgc
     2221 ctcggaccgg tcggcgagat ccttgcgcag ccgcgagagc cgggcgcggg acgcctcgtc
     2281 ggactccttc tcgagcgcca gctcctccat cttgagccgg tcgacggcgc ggcggagctc
     2341 gtcgatctcg accggtgagg agtcgatctc catccgcagc cgcgaggcgg cctcgtcgac
     2401 gaggtcgatc gccttgtccg gcagctggcg cccggagatg taccggtcgg acagcgtcgc
     2461 ggccgccacg agcgcagagt cggcgatggt gaccttgtgg tgcgcctcgt agcgctcctt
     2521 gagcccgcgc aggatcccga tcgtgtcctc gacgctcggc tccccgacga agatctgctg
     2581 gaaacgccgc tcgagcgcgg cgtccttctc gatgtgcttg cggtactcgt cgagcgtcgt
     2641 ggcaccgacc atgcgcagct cgccccgggc cagcagtggc ttgagcatgt tgcccgcgtc
     2701 catcgagccc tcgccagtcg cgcccgcacc gacgacggtg tgcagctcgt cgatgaacgt
     2761 gacgacctgg ccgttcgagc tcttgatctc ctcgagcacg gccttgagcc gctcctcgaa
     2821 ctcgccccgg tacttcgcgc cggcgaccat cgcaccgagg tcgagcgcga tgagggtctt
     2881 gccccgcagg ctctcgggca cgtccccggc gaccatgcgc tgggccagcc cctcgaccac
     2941 cgcggtcttg ccgacgccgg gttcgccgat gaggacgggg ttgttcttcg tccgccggga
     3001 cagcacctgg acgacccggc ggatctcggc gtcgcgcccg atgacggggt cgagcttgcc
     3061 ctcccgggcc tgggcggtga ggtcggtgcc gtacttgccc agcgcgtcga acgtcgcctc
     3121 agggttctcc gtggtcacct tggccccacc acgcagagcc ggtatggcgg cctcgatcgc
     3181 cgccgggtcg aggccgaact gacgggaccg ggcgagcccg agcacgagat ggtcggtcga
     3241 gacgtaggtg tcgcccatcg cggacatggc cgcacgggcg ttctccaggg ccgccagcga
     3301 accccgcgac agaccggggg tggccatcga ggaaccggtg gcggacggca taccggacag
     3361 ggtcttggcg gccgcctgag ccagcgcccg ggccgacgtg cccgcggcgg tgagcagggc
     3421 cggagtggtc gtctcggcct gctcggccag cgccttgagc aggtgcgcgg gctcggtctg
     3481 ggcgtggccg ctcgcggccg cgtcgcggac cgcagccgag agcgcttccg acgccttggt
     3541 cgtcaggtgc agatccatgg cagtggctcc tgtcgttggc gcggggatgc gtcgacggcg
     3601 acaacctcgc caaagttgag tctattccac tcaatgttgg ctgaggtggg gcccggcctc
     3661 gaccgacctc acgggcgggc acatcgacgg aggtcggctc gcctgaccgg atcgaggcct
     3721 cacccgctac gtcgggacca gtgcggcgat ccccgcggcc gacagcgccc ggttgtagat
     3781 ccgcacgtcg tccagctggc cgtcccagaa cgcttccagc agacctttcg agggcgcgtt
     3841 cttggcgccg atcgccagcg gctcgttgtt cgagcacgtg cggcccgtca cggccatgct
     3901 tcccgacttg ctgccattga tccacaccgt cagcgtcgag ccgttgtacg tcgccgccag
     3961 gtgcgtccac gtgttgaggg gaaccgcggt agtgctgtcg aagaacttcc aggccgacgt
     4021 cgcggtgcag ctgccgtaga agccacccac ccgcaccctg ccgttggtcc gcacgttcat
     4081 ccagtaggcg ccggccttct cgagcacctc ccagcgaccg agggtctggt cgttggggat
     4141 gcccgtatag cgcaccagcg cggtgagcgt gtagcggtcg acgtcgagcg cgttgccctc
     4201 gggcacggcg acgaactggt ggccggcccc gaccagggcg acggccgtgc catgtcccgg
     4261 cacgctggcc gacgttgccg ccgcggcagt cgtgttcacc agcatgccgt ccagcctgct
     4321 gggcgagacg tcgcgcacga tcccacccgc caggttctcg aacgggtagc tcaggacgat
     4381 ggcactgggt acggcggcac tgggtacggc ggcagtggcc ggggcactga caagcgcggc
     4441 ggccaggccc gcagcgaccg caccgatgac gacggtccgc acgagacctc cgtccagtcc
     4501 caaggcaaga acacttcggc caggctgacg gtatcgcacg tacgtgccga gcgcgtcgat
     4561 cggtcagcgc ccgagccgac gcccccacgg cagtgagcag tgcgggagtc gtcgcctcgg
     4621 cctgctcggc cagcgccctg agcaggtgcg cgggctcggt ccgggcgtgg ccgctcgcgg
     4681 cagcgtcgcg gaccgcagcc gacagccttc ctcatgttcg ccatgccgag tcattgcgtg
     4741 acccgacggc gccgctcgag gacgatcagg cacgcgccaa cgatgatgag tgagacggcg
     4801 cccccggtga gatccgtagt gcgcgatccg gtgtaggcca gtgaccgtcc cagcggcgcg
     4861 gcctggatc
//
LOCUS       DATAIX010000673         3264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_138276, whole genome shotgun sequence.
ACCESSION   DATAIX010000673 DATAIX010000000
VERSION     DATAIX010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138276"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2045)
                     /locus_tag="VHM65_09890"
     CDS             complement(<1..2045)
                     /locus_tag="VHM65_09890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HEX2706058.1"
                     /translation="MQRVDGRLVLSPTDLTKHVSCPHITTLDLRRLDGEPGLEPAAPD
                     DALALVFTKGIEHEERHLAGLRERGLRVVEIDTSVGKQAAESATLAAMRGGADVVYQA
                     TLYDGAWVGHADFLLKTRRPSNLGEWSYDLADTKLARRLKVPALLQMATYAVRLADLQ
                     GAPPRQLVVVTGDRAEHPWRLVDVESYAARLRDRLVRAVHERPPTEPVPVAQCAQCRW
                     LDTCAAEWRRRDDLSQVAGMRVDQRQALCDSGIRTMTQLAGMAADDLEGILSAATRQR
                     LVAQARLQHHERISGGPAYELLDPEPGQGLARLPEPSPGDVYLDFEGDPWAEGGAGRE
                     YLAGLWERSGEFTAWWAHSAAEEKRLTGDLVDELIRRWQGDPQMHIYHYAAYEVTALK
                     RLTGRHGTHEAELDQLLRSERFVDLYAVVRQGVRISKASYSIKKLEDFYWGRTRSAGT
                     EQVTDAMTSIVDYERWLVDADQSTLDRILAYNREDCRSTHDLQAWLEDRRAELSGQGV
                     EVPRPTPPEPKEVTDEERAEAALAERLLDAGHALLAGCVGWHRREARPAWWDYFHYSE
                     LDTEALIEDATAIGALGEPAARGAVKRSTVWRYPFPAQDCRPVVGDYAPDVDTHMNVG
                     KVLGYDPVAGWVDLSIGNGRAPVVPRGLGVKPPIVDGVLRTSIARTAERVLDGQSPLG
                     "
     gene            complement(2171..2905)
                     /locus_tag="VHM65_09895"
     CDS             complement(2171..2905)
                     /locus_tag="VHM65_09895"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX2706059.1"
                     /translation="MSLLASWTQRSHSADGYTHDTYRKGSGPGVIVIHEVPGLHPSVI
                     EFGEELVEAGYTVLMPSLFGRAEAPYSAWSMVKALPRICISRELTLLATGKTAPLAGW
                     LRSLARELHADVGGPGVGALGMCLTGGFALAMMVDPAVIAPVLCQPSAPLPLGDARAG
                     DVNLSPSDVVAVRERTRSGCAVLGLHFAEDPLVGTRFETLRRGLGPSFREVPLPGKGH
                     STVTEQRSQVAVDAILAFFAERLRGG"
     gene            complement(2902..3120)
                     /locus_tag="VHM65_09900"
     CDS             complement(2902..3120)
                     /locus_tag="VHM65_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX2706060.1"
                     /translation="MVESLLAQPGTVVAEPTIRHTSILRGLLEQAGTAGNLTTDAHVA
                     AIAIEHGATIATFDRDSDPAGHLGRWAA"
BASE COUNT          464 a         1149 c         1167 g          484 t
ORIGIN      
        1 ccgagtgggc tctgcccgtc gaggacgcgc tcggccgtcc gcgcgatgga ggtccgcaga
       61 accccgtcga cgatcggtgg cttgaccccg agcccgcggg ggacgacggg cgccctcccg
      121 ttgccgatgg acaggtccac ccatccggcg accgggtcgt agccgagcac cttgccgacg
      181 ttcatgtggg tgtcgacgtc gggcgcatag tcgcccacca ccggacggca gtcctgcgcc
      241 gggaacgggt agcgccatac ggtggatcgt ttgaccgcgc cccgcgccgc cggctcgccc
      301 agcgcgccga tggccgtcgc gtcctcgatg agcgcctcgg tgtcgagctc ggagtagtgg
      361 aagtagtccc accacgccgg gcgcgcctca cggcggtgcc accccacaca gcccgcgagc
      421 agggcatgac cggcgtcgag cagccgctcg gccagcgccg cctcggcccg ctcctcgtca
      481 gtgacctcct tgggctcggg cggcgtgggg cgtggcacct cgacgccctg accgctgagc
      541 tcggcccgcc ggtcctcgag ccaggcctgc aggtcgtggg tcgagcggca gtcctctcgg
      601 ttgtaggcga ggatccggtc gagagtcgac tggtcggcat cgacgagcca gcgttcgtag
      661 tcgacgatgg aggtcatcgc gtcggtgacc tgctcggtcc ccgccgaccg ggtccggccc
      721 cagtagaagt cctcgagctt cttgatcgag taggacgcct tgctgatccg gacgccttgg
      781 cggaccacgg catacaggtc gacgaaccgc tcgctgcgca gcagctggtc gagctcggcc
      841 tcgtgcgtgc catggcgccc cgtgagccgc ttgagcgcgg tgacctcata ggctgcgtag
      901 tggtagatgt gcatctgcgg gtcgccctgc caccggcgga tcagctcgtc gacgaggtcg
      961 cccgtcagcc gcttctcctc ggccgcgctg tgggcccacc aggcggtgaa ctcaccgctg
     1021 cgctcccaga gcccggcgag gtactcacgg cccgcacccc cctcggccca ggggtcgccc
     1081 tcgaagtcga gatagacatc ccccgggctg ggttcgggca gccgcgcgag tccctgcccc
     1141 ggctcagggt cgagcagctc gtatgccggg ccgccagaga tccgctcgtg gtgctgcagc
     1201 ctcgcctggg cgacgagccg ctgccgagtg gcggcagaca ggatcccctc gaggtcatcg
     1261 gcggccatcc cggcaagctg ggtcatcgtg cgaatcccgc tgtcgcacaa ggcctgccgc
     1321 tggtcaacgc gcatcccggc aacctggctg aggtcgtcgc ggcggcgcca ctcggccgcg
     1381 caggtgtcga gccagcggca ctgggcgcac tgggccaccg ggacgggctc ggtcggcgga
     1441 cgttcatgga cggcccggac gagccggtcg cgcaggcgcg cggcatagga ctcgacatcg
     1501 acgaggcgcc acggatgctc ggcccggtcg ccggtgacga cgaccagctg acgcggtggc
     1561 gcgccctgca ggtcggcgag ccgcacggca tacgtggcca tctgcaggag tgcgggcacc
     1621 ttgagccggc gcgcgagctt ggtgtcggcg aggtcgtagc tccactcccc caggttgctc
     1681 ggccgccgtg tcttgagcag gaagtcggcg tggccgaccc aggctccgtc atacaacgtg
     1741 gcctggtaga cgacgtccgc gccaccgcgc atggccgcca gcgtggcgct ctcggcggcc
     1801 tgcttgccga ccgacgtgtc gatctcgacg acccgcagcc ctcgctcgcg cagccccgcg
     1861 aggtggcgct cctcgtgctc gatgcccttg gtgaagacga gcgcgagggc gtcgtcgggg
     1921 gctgcgggct cgagaccggg ctcgccgtcg aggcgacgca ggtcgagagt cgtgatgtgc
     1981 gggcaggaga cgtgcttggt caggtcggtg gggctcagga ccagccgtcc atccactcgc
     2041 tgcatgtcgc gctccgtcgt ccagatgtcg tggggactcc cccgacggcg agtatggcgc
     2101 cgaccaccga cagcacatcg cctaccccgg cgccggcgcc ggggtaggcg ggccgagcgg
     2161 gcgggccggc tcagccgccg cgaaggcgct cggcgaagaa ggcgaggatg gcgtcgacgg
     2221 ccacttggct gcgttgctcg gtcaccgtcg agtggccctt cccgggcagg gggacctcgc
     2281 ggaagctcgg cccgagcccg cgcctgaggg tctcgaagcg cgtgcccacc agtgggtcct
     2341 cggcgaagtg caggccgagg accgcgcagc ccgaccgagt ccgctcgcgg accgccacga
     2401 cgtcgctggg gctgaggttg acatcgccag ccctggcgtc acccagtggc agaggagccg
     2461 agggctggca gagcaccgga gcgatcaccg cggggtcgac catcatggcc agtgcgaagc
     2521 caccggtcag gcacatcccc agcgcgccga ctccggggcc accgacgtcg gcatgcagct
     2581 cacgggccag actgcgcagc cagcccgcaa gcggcgcggt tttgcccgtc gcgaggaggg
     2641 tgagctcccg gctgatacag atccgcggca gcgccttgac catcgaccag gccgagtagg
     2701 gcgcctcggc cctcccgaac aggctcggca tgagcacggt gtaccccgct tcgacgagct
     2761 cctcgccgaa ctcgatgacg ctcggatgca gccccgggac ctcgtggatg acgatgaccc
     2821 ccggtcccga gcccttgcgg taggtgtcat gggtgtagcc gtcagcgctg tggctgcgct
     2881 gggtccagga ggcaagcagg ctcatgcggc ccatcgtccc aggtgacccg cgggatcgga
     2941 gtcccggtcg aacgtcgcga tggtcgcgcc atgctcgatc gcgatggcag ccacgtgggc
     3001 gtccgtcgtg aggttgcccg ccgtcccggc ctgctcgagg agtccacgca agatagaggt
     3061 atggcggatc gtgggctcag ccacgacggt ccctggctgc gccagcaacg actcgaccaa
     3121 cgccgtcgac tgggcgatgc atcattgcct tgatgccttg atgcttgcta gggacaggtc
     3181 tcaccgcgcc tggtcagctc gatatccagg gcctgagtgg cgtagccatt gccgtccata
     3241 tgggccgacc agcagccctt gaac
//
LOCUS       DATAIX010000674         2947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_203105, whole genome shotgun sequence.
ACCESSION   DATAIX010000674 DATAIX010000000
VERSION     DATAIX010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203105"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VHM65_09905"
     CDS             complement(<1..420)
                     /locus_tag="VHM65_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706061.1"
                     /translation="MSVYDHSADLSYNPSPPAPSADPPSDLAPKPQVQEGAPGSSGRR
                     EVSLVFLGASMTAGYGDPKGLGWVGRVVIRTLHPDLDLTAYNLGVRGQTSAEVMSRWR
                     AECAPRWRGERRLVVSVGAADVSEDLSLARSRLNLANI"
     gene            complement(452..628)
                     /locus_tag="VHM65_09910"
     CDS             complement(452..628)
                     /locus_tag="VHM65_09910"
                     /inference="COORDINATES: protein motif:HMM:NF039659.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6104 family protein"
                     /protein_id="HEX2706062.1"
                     /translation="MYFTDRGIEELAARRGDEQVSFAWLADALRTFVDVHPELETPVE
                     RLATWLARFDDDAE"
     gene            complement(658..>2947)
                     /locus_tag="VHM65_09915"
     CDS             complement(658..>2947)
                     /locus_tag="VHM65_09915"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEX2706063.1"
                     /translation="ASADEQLRILRRLNSAEAFETFLQTKFVGQKRFSLEGGESVIAL
                     LDRILCRAAADDMHEVCIGLPHRGRLNVLANIAGKSYAQIFREFEGKQDPASVQGSGD
                     VKYHLGTEGQFVSEDAKKTRVYLAANPSHLEAVDPVLEGIVRAKQDRLNLGGEAFTVL
                     PVLMHGDAAFAGQGVVAETLNLSQLRGYRTGGTIHVVINNQVGFTTSPASSRSSVYCT
                     DVARMIQAPIFHVNGDDPEACVRVAELAYDFRQEFAKDVVVDMVCYRRRGHNEGDDPS
                     MTQPLMYTLIEAKRSVRKLYTENLIGRGDLSVEDAEAALRDYQQQLERVFIETREATA
                     TGGPSQTPPEDRGTDADGHGGLGKPTAQIFDEANSPHSATETGISAEMLLHLGDSFVR
                     PPEGFTVHPKLRQLMEKRADMVRSGGIDWGMGELLAFGSLLMEGTPVRLAGQDSRRGT
                     FVQRHAVLIDKVSAKEWTPLLYLDRGQAKFWVYDSLLSEYAAMGFEYGYSVERPDALV
                     LWEAQFGDFANGAQSIIDEFISSSEQKWGQRSSVVLLLPHGYEGQGPDHSSARIERYL
                     QMCAEDNMTVAYPSTPASYFHLLRRQAYARPRRPLIVFTPKSMLRLKAASSRPEELTT
                     GGFRPVLPDPLALDKGAVTQVLLASGKVVHDLDAERAKRDDTATAIMRVEQLAPVPAQ
                     DVAHALHDYPNAEVVWVQDEPQNQGPWPFMALNLPGALAGFGQSRPLRVVSRKASASP
                     ATGSHKKHEIEQAELIARAFDR"
BASE COUNT          432 a          953 c         1096 g          466 t
ORIGIN      
        1 gatgttggcg aggttgagcc gcgatcgggc gagggagagg tcctcggaga catcggccgc
       61 gccgaccgag accacgagcc gacgctcccc gcgccaccgc ggcgcgcact cggctcgcca
      121 gcggctcatg acctcggccg aggtctgccc gcggacgccc aggttgtatg ccgtgagatc
      181 caggtccggg tgcagcgtcc ggatgacgac gcgaccgacc cacccgaggc ccttcgggtc
      241 gccatacccg gccgtcatcg acgcaccgag gaagacaagt gacacctcgc gccggcccga
      301 gctgcccggg gcgccctcct ggacctgcgg cttgggagcc aggtcgctgg gtgggtccgc
      361 gctgggcgct ggcggcgacg ggttgtagga caggtcggca ctgtggtcgt agacactcat
      421 cggctactca tcccgtcgtc gttgcgcgtc atcattcggc gtcgtcgtcg aaccgggcga
      481 gccaggtcgc gagacgctcc accggtgtct cgagctctgg gtggacgtcg acgaaggtcc
      541 gcagcgcgtc ggccagccag gcgaaactga cctgctcgtc gcctcgtcgg gcggcgagct
      601 cctcgatgcc ccggtcggtg aagtacacag gaccgaggct atctcccggg gtgcggctca
      661 gcggtcgaac gcgcgggcga tgagctcggc ctgctcgatc tcgtgtttct tgtgcgaccc
      721 ggtggcgggg gaggccgagg ccttgcggga gacgactcgc agcggccgac tctggccgaa
      781 cccggcgagc gccccgggca ggttcagggc catgaacggc cagggaccct ggttctgggg
      841 ctcgtcctgg acccagacga cctcggcgtt tgggtagtcg tgcagggcat gtgccacgtc
      901 ctgggcgggg acgggcgcga gctgctcgac ccgcatgatc gcggtggccg tgtcgtcgcg
      961 cttggcgcgc tcggcgtcca ggtcgtggac gaccttgccc gaggcgagga ggacctgggt
     1021 aacggcgccc ttgtcgagcg cgaggggatc ggggagcacc ggccggaagc caccggtcgt
     1081 gagctcctcg ggccggctcg aggcggcctt gagccgcagc atcgacttcg gggtgaagac
     1141 gatgagcggc cggcgcgggc gggcataggc ctgacggcgc agcaggtgga agtacgaggc
     1201 cggggtcgac gggtaggcca cggtcatgtt gtcctccgcg cacatctgca gatagcgctc
     1261 gatccgcgcc gagctgtggt ccggcccctg gccttcgtag ccgtggggca ggagcaggac
     1321 gaccgaggaa cgctgtcccc acttctgctc cgaggaggag atgaactcgt cgatgatgga
     1381 ctgggcgccg ttggcgaaat cgccgaactg ggcctcccac aggacgaggg cgtcggggcg
     1441 ctcgaccgag tagccgtact cgaagcccat cgcggcatac tcgctgagca gcgagtcgta
     1501 cacccagaac ttcgcctggc cgcggtcgag gtagagcagc ggggtccact ccttggcgct
     1561 gaccttgtcg atgaggaccg cgtgccgctg gacgaacgtg ccgcggcggc tgtcctgtcc
     1621 cgcgaggcgc accggcgtgc cctccatgag cagggacccg aaggcgagca gctcgcccat
     1681 gccccagtcg atcccaccgc tgcggaccat atcggcccgc ttctccatga gctggcgcag
     1741 cttgggatgg accgtgaacc cctcgggcgg ccggacgaag gagtcgccga ggtggagcag
     1801 catctcggcg gagatcccgg tctcggtcgc cgagtgcggg ctgttggcct cgtcgaagat
     1861 ctgcgcggtc ggcttgccca gaccgccgtg gccgtctgca tcggtgccgc ggtcctcggg
     1921 cggcgtctgc gaggggccac ctgtggcggt cgcctcgcgg gtctcgataa agacccgttc
     1981 gagctgctgc tggtagtcgc gcagggcggc ctcggcgtcc tcgacggaga ggtcaccgcg
     2041 gccgatgagg ttctcggtgt acagcttgcg caccgagcgc ttcgcctcga tgagggtgta
     2101 catcagcggc tgggtcatcg acgggtcgtc gccctcgttg tggccccggc gccggtagca
     2161 caccatgtcg acgacgacgt ccttggcgaa ctcctggcgg aagtcgtagg ccagctcggc
     2221 gacgcggacg cacgcctcag gatcgtcgcc gttgacgtgg aagatcggtg cctggatcat
     2281 ccgggcgacg tcggtgcagt agaccgagga ccgcgacgac gccggcgagg tggtgaaccc
     2341 gacctggttg ttgatgacga cgtggatggt cccgccggtg cggtagcccc gcagctggga
     2401 caggttgagg gtctcggcga cgacgccctg gccggcgaag gcggcgtccc cgtgcatgag
     2461 gacgggaagc acggtgaagg cctcgccgcc gaggttgagc cggtcctgct tggcgcggac
     2521 gatcccttcc aggaccgggt cgacggcctc gaggtgagag gggttcgcgg cgaggtagac
     2581 cctggtcttc ttggcgtcct cggagacgaa ctggccctcg gtgccgaggt ggtacttcac
     2641 gtcgccggag ccctggaccg aggcggggtc ctgctttccc tcgaactcgc ggaagatctg
     2701 cgcgtaggac ttgcccgcga tgttggcgag cacgttgaga cgcccacggt ggggcaggcc
     2761 gatgcacacc tcgtgcatgt cgtcggcggc ggcccggcac aggatccggt cgagcagggc
     2821 gatcacggac tcgccgccct cgaggctgaa acgcttctgc ccgacgaact tggtctgaag
     2881 gaacgtctcg aaggcctcag cggagttgag ccgcctcagg atccgcagct gctcgtcggc
     2941 agaggcc
//
LOCUS       DATAIX010000675         6890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_257742, whole genome shotgun sequence.
ACCESSION   DATAIX010000675 DATAIX010000000
VERSION     DATAIX010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257742"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1399
                     /locus_tag="VHM65_09920"
     CDS             <1..1399
                     /locus_tag="VHM65_09920"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF002550.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEX2706064.1"
                     /translation="SSINGMIFQRGNPMDFERWGREPGMATWDYAHCLPYFNRMETCL
                     AAAPDDERRGHAGPLVLERGPVTNPLFTAFLESVQEAGYPLTDDVNGYRQEGFSAFDR
                     TIHKGRRLSAAGAYLHPALTRPNLTLVTRAQASRVLFDGTRAVGVEYLRRGRTVTVRA
                     KDVILCGGAINSPALLQLSGVGDAGHLDSLGIPLVHHLPGVGENLQDHLEAYVQYGCK
                     EPVSLQPMMTHKFRYGPWIGLRWLLFRTGPGASNHFEAGGFARSNEDVSYPNLMFHFL
                     PVAVRYDGSAPAGGHGFQLHVGPMYSDAIGSVKITSRNPSTKPALRFNYLSTAQDRRE
                     WVEAVRVARSLIDTPAMSRYSTGEISPGPDVRTDEQILHWVASDAETALHPSCTCKMG
                     DITADPMAVVDPTTMKVHGLEGIRVVDASVMPYVTNGNIYAPVMMIAEKAADLILGNA
                     PLPPSGADFYRHTTA"
     gene            1573..1962
                     /locus_tag="VHM65_09925"
     CDS             1573..1962
                     /locus_tag="VHM65_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706065.1"
                     /translation="MSTIATRVAALAAATAAAGVLAVGAAGSASAQQYLGTRLQPSQN
                     TCVSQYAGYQFQATGTATGSGARFKVLYNGQIVRDSYGRVGYFTAEGRSSYGTFPGPG
                     YYSLCAFNTGSAKTDVTLNLRTDGEVW"
     gene            2139..4205
                     /locus_tag="VHM65_09930"
     CDS             2139..4205
                     /locus_tag="VHM65_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706066.1"
                     /translation="MGSTVGDRLHAARQRGFVGRAAELTLFDEIVTAADLPITVLWVH
                     GPAGVGKSTLLRRYAQATADHAVACLSVDARDLAPSIESLMAAFGPVVTTPPDATAAE
                     ATASAAGRRVLMIDTFELLESAEELLRSSVLPHLPANLLVVVAGQYPPGPAWRADPGW
                     AELLRPVPLENLSAQEASDYLVARGVADPERTRAVEFTRGHPLALALVSEVIRTRGSF
                     DPKGSPDVIAMLVEALVQGVPTSLHRRALQASAQVRVVTEALLTCLLDIPDAADLFDW
                     LRRLPVVDTGSSGLYLHDLAREVLAADLKWRHPERYLELHERARAHFLARLDSPGAAS
                     QATALIDLIFLHDELRPYLQPVESSSGGLLMDRPRDDERPAIRAMIGSHEGEESAAIA
                     EQWLDRQRDAWQVVRDSAGQLLGILCTLAIEGVADDDAGTDPAVAAARRELAHHPPLR
                     DGERATLFRFWMSRDGYQSVSPVQSLIAVQLGRHYLTTAGLALTLLPFADPQAWAQIA
                     LYADQHPMPSAAFTVGGRAYGVFGHDWRVVPPTSWLATLARREIGIAPGAAPPDLSSS
                     VAVLTREDFAAAVRQGLRGLTRPDRLRRNPLLAARIVTTKVAAGAPARERVAALQAAL
                     REACLSLEGDRRAYRVLHRSFLAPAPTLEAAAEALSLPSSTFRRHLTAAVQRVVDLLW
                     EQELTP"
     gene            complement(4382..5374)
                     /locus_tag="VHM65_09935"
     CDS             complement(4382..5374)
                     /locus_tag="VHM65_09935"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2706067.1"
                     /translation="MKQFSKHVRGWRRTAVLASAAALTLAGCGGAKVGETPAAAGSSS
                     GGKVACGTFNIAINPWVGYEANAAVIGYVAEKELGCKVVKKDLKEEVAWQGFGSGEVD
                     AVVENWGHDDLKKKYVEGQKTAVSAGETGNKGIIGWYVAPWMAEKYPDIIDSKNLNKY
                     ADLFKTSESKGKGQLLDGDPSFVTNDAALVKNLGLDFEVVYAGSETALIQAFRTSEKD
                     KKPILGYFYEPQWFISELALKKVTLPAYTAGCDADAAKVACDYPEYALDKIVAKKFAE
                     ANGPAYQLVKSFTWTNEDQNSVAKSITEDKLTPEAAAKKWVDANADKVKAWIAG"
     gene            complement(5495..>6890)
                     /locus_tag="VHM65_09940"
     CDS             complement(5495..>6890)
                     /locus_tag="VHM65_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2706068.1"
                     /translation="CYLAPLTLFFLIGPAAAAIATLIYATPPVIRLTAHGIRTVPATT
                     LEATDSLGATTWQSLTQVMLPMAKRTIVLGANQTIMAALSMATITALIAAPGLGKVVI
                     KALQSLDVGQSVNGGLAIVVMAIVLDRVTTAASVRAQRVPKTGRDLHLRRVVVGVGAA
                     LTAVAAYLSNTFVWAAEFPAEPTLGPTIRTWSENATEWVQDTFAPVTNAFKDIVTAYL
                     LDPLQALLSESPWWLALVALVCLAGVLGGRRAVVTTSICLLLIVGLGLWNDAMVTLAS
                     TLLATIMVMAFGVVVGVWMGRSRRVDQAIRPILDAGQTLPAFVYLVPFLALFAASRFT
                     AIAAALVYAAPASIKIIADGVRGLSATTMEAAASTGATAWQTIRLVQLPMAAKALALG
                     ANQGIIFVLAMVVVGGLVGAGALGYDVVAGLVQGQLYGKGLAAGVAISLLGIMLDRIT
                     QSAARRVDPSRPRG"
BASE COUNT         1009 a         2526 c         2332 g         1023 t
ORIGIN      
        1 gagctcgatc aacggcatga tcttccagcg gggcaacccg atggacttcg agcgctgggg
       61 acgcgagccc ggtatggcga cctgggacta cgcgcactgc ctgccctact tcaaccggat
      121 ggagacgtgc ctggccgccg cccctgacga cgagcgccgt ggacatgcgg gacccctggt
      181 cctggaacgc ggaccggtga cgaacccgct cttcacggcg ttcctggagt cggtccagga
      241 ggccggctac ccgctcaccg acgacgtcaa cggctaccgc caggagggct tcagcgcctt
      301 cgaccgcacg atccacaagg gccggcgcct ctcggcggcg ggggcctacc tgcaccccgc
      361 actcacccgg cccaacctca ctctcgtcac ccgcgcccag gcgagccggg tcctcttcga
      421 cggcacgcgg gccgtggggg tcgagtacct ccgccgtggc cgcacggtga cggtccgcgc
      481 caaggacgtc atcctctgcg gcggcgccat caactcccct gcgctgctcc agctctcggg
      541 tgtcggcgat gccggacacc tcgacagtct cgggattccg ctcgtccatc acctgcccgg
      601 agtgggggag aacctccagg accacctcga ggcctacgtg cagtacggct gcaaggagcc
      661 cgtctcgctg cagcccatga tgacccacaa gttccgctac ggaccgtgga tcggcctgcg
      721 ctggctgctg tttcgcaccg gccccggcgc ctccaaccac ttcgaggccg gggggttcgc
      781 ccgcagcaac gaggacgtct cctaccccaa cctgatgttc cacttcctcc cggtcgccgt
      841 ccggtatgac gggtcggcgc ccgcgggtgg gcacggcttc cagctgcacg tcgggccgat
      901 gtactccgac gcgatcggtt cggtgaagat cacgtcgagg aacccgtcca ccaagccggc
      961 actgcgcttc aactaccttt cgaccgcgca ggaccgccgc gaatgggtcg aggcggtccg
     1021 ggtggcccgc tcgctgatcg acacaccggc catgtcccgc tacagcacgg gcgagatctc
     1081 ccctgggccc gacgtcagga ccgacgagca gatcctgcac tgggtcgcca gtgacgccga
     1141 gaccgccctg cacccctcgt gcacctgcaa gatgggcgac atcaccgccg accccatggc
     1201 agtcgtcgac cccacgacga tgaaggtcca tggcctggaa ggcattcggg tggtcgacgc
     1261 ctcggtcatg ccgtacgtga cgaacggcaa catctacgcc ccggtgatga tgatcgcgga
     1321 gaaggccgcc gacctcatcc tcggcaacgc gccgctgccc ccctcgggag ccgacttcta
     1381 ccgccatacc accgcgtgac gtgtcccgga gcccatgggc gaagcgggtg gcgggaagtg
     1441 agcgggatct gagcgggtcc tggtctgggg agtgagcgcg gggccgacct accttcgggt
     1501 catcagcagg gggccacacc cggaccccac cgcgacgggc cacccgcccg cagacccaga
     1561 aggagtacca ccatgtccac gatcgccacc cgcgtcgccg ccctcgccgc cgccactgct
     1621 gcggcgggcg tcctcgccgt cggagccgcc ggttccgcct ccgcccagca gtacctcggc
     1681 acccgtctgc agccgagcca gaacacgtgc gtgtcgcagt acgccggcta ccagttccag
     1741 gccaccggaa cggcgactgg cagcggcgcc cggttcaagg tcctctacaa cgggcagatc
     1801 gtccgggaca gctatggccg cgtggggtac ttcaccgccg aggggcgctc gtcctacggg
     1861 accttcccgg gcccgggcta ctactcgctg tgcgccttca acaccggcag cgccaagacc
     1921 gacgtcacac tgaacctgcg gaccgacgga gaggtctggt agccgctccg gcgcggcacc
     1981 accaggcggc acctctccgt ggggccgggt ccaccctcgt gggcccggcc cccatcgtgt
     2041 tgccaccact gtcgccctgg ggcaccatga gtgcagtcca cgtgaggccg cgcgccgcgg
     2101 cgcaggccag ccgagcgcca tacccaaggt ggtgatccat gggcagcacg gtcggggacc
     2161 gcctccacgc cgcccgccaa cgtgggttcg tcgggcgcgc ggccgagctg acgctcttcg
     2221 atgagatcgt cacggccgcg gacctgccca tcaccgtgct gtgggtccac gggccggccg
     2281 gcgtgggcaa gagcacgctg ctgcgccggt acgcgcaggc caccgccgac cacgctgttg
     2341 cctgcctcag tgtcgatgcc cgcgacctcg caccctcgat cgagtcgctc atggccgcct
     2401 tcggtcccgt cgtcacgacg ccaccggatg ccacggcagc ggaggcgacg gcgtccgccg
     2461 ctgggcggcg ggtgctcatg atcgacacgt tcgagctgct cgagagcgcc gaggagctgc
     2521 tgcgcagctc ggtgctgccc caccttcccg ccaacctgct cgtcgtcgtc gccggccagt
     2581 accccccggg cccggcctgg cgagccgacc cgggatgggc tgagctgctc cggccggtcc
     2641 cactcgagaa cctctcggcg caggaggcgt cggactacct cgtggcccgc ggcgtggccg
     2701 accccgagcg cacccgggca gtcgagttca cccgtggcca tccgctcgcg ctcgcgctcg
     2761 tcagcgaggt gatccgcact cgcgggtcgt tcgaccccaa gggctccccc gatgtcatcg
     2821 ccatgctcgt cgaggctctg gttcagggtg tcccgacgag cctgcaccgc cgagcgcttc
     2881 aggccagcgc ccaggtccgg gtcgtcaccg aggcgctgct cacctgcttg ctcgacatcc
     2941 ccgacgcggc ggacctcttc gactggctcc ggcggctgcc cgtcgtcgac acgggctcct
     3001 ccggtctcta cctgcacgac ctggcccgcg aggtcctcgc cgccgacctc aagtggcgcc
     3061 accccgagcg gtacctcgag ctgcacgagc gggcgcgggc ccacttcctg gcccggctcg
     3121 acagcccggg cgcggccagc caggccaccg ccctgatcga cctgatcttc ctccacgacg
     3181 agctgcgccc ctacctgcag cccgtcgagt cctcgtcggg cggcctgctc atggaccggc
     3241 cacgcgacga cgagcggccc gccatccgtg ccatgatcgg gtcacacgag ggcgaggagt
     3301 cagccgccat cgccgagcag tggctcgacc gtcagcgaga cgcctggcag gtggtccgtg
     3361 actctgccgg gcagctcctc gggatcctgt gcacgctggc catcgagggc gtggccgacg
     3421 acgacgcggg caccgacccg gcggtggcgg cggcgcgccg cgagctcgct catcacccgc
     3481 cgctgcgcga cggcgaacgc gccaccctct tccggttctg gatgagccgg gacggctacc
     3541 agagcgtctc gccggtgcag agcctcatcg ccgttcagct gggccgccac tacctcacca
     3601 ccgcgggcct ggccctcacc ctcctgccgt tcgccgaccc gcaggcgtgg gcgcagatcg
     3661 cgctctacgc ggaccagcac cccatgccat cggccgcctt cacggtcggc gggcgcgcgt
     3721 atggcgtctt cggtcacgac tggcgggtcg tgccgccgac gtcgtggctc gcgacgctgg
     3781 cccgccggga gatcggcatc gcccccgggg ccgccccacc ggacctcagc tcgtccgtcg
     3841 ccgtgctcac ccgtgaggac ttcgcggccg cagtccgcca gggcctgcgg gggctgacgc
     3901 gaccggaccg gctgcgcagg aaccccctgc tggccgcccg gatcgtcacg acgaaggtcg
     3961 cggccggtgc ccccgctcgc gaacgggttg ccgcgctcca ggccgcgcta cgggaggcgt
     4021 gcctgtcgct ggagggcgac cggcgggcct accgcgtcct gcatcggtcg ttccttgccc
     4081 ccgcgccgac gctcgaggcg gccgctgagg cgctgagcct gccgtcgagc accttccgac
     4141 ggcacctgac ggcggcggtc cagcgggtcg tcgacctcct gtgggagcag gagctcaccc
     4201 cctgaggtcc gtagggctga ccctggcact ccccacgcga accgcaggac tggtcgaacg
     4261 tgcaccagac ccgaacaaac tggggcattt cgtccacaat tagtccgtta ggccagttca
     4321 ggtgtgcgac caactgcgct cgctcaccca tccggcccgg cccggcccaa cgggcgtcag
     4381 gtcagccggc gatccaggcc ttgaccttgt ccgcgttggc gtcgacccac ttcttcgccg
     4441 ccgcctcggg cgtgagcttg tcctcggtga tcgacttggc gacggagttc tggtcctcgt
     4501 tggtccaggt gaagctcttg accagctggt aggccggccc gttcgcctcg gcgaacttct
     4561 tggcgacgat cttgtcgagg gcgtactcgg ggtagtcgca ggcgaccttg gccgcatcgg
     4621 cgtcgcagcc cgcggtgtag gccggcagcg tgaccttctt gagcgcgagc tcggagatga
     4681 accactgcgg ctcgtagaag tagccgagga tgggcttctt gtccttctcg gacgtgcgga
     4741 acgcctggat cagcgcggtc tcgctgccgg catacacgac ctcgaagtcg agcccgaggt
     4801 tcttcacgag cgctgcgtcg ttggtgacga aggacggatc accgtcgagg agctggccct
     4861 tgcccttgga ctccgacgtc ttgaacaggt cggcgtactt gttgaggttc ttcgagtcga
     4921 tgatgtccgg gtacttctcg gccatccacg gggcgacata ccagccgatg atgcccttgt
     4981 tgccggtctc gccggccgac accgcagtct tctgtccctc gacgtacttc ttcttcaggt
     5041 cgtcgtgacc ccagttctcg acgacggcgt cgacctcacc ggagccgaag ccctgccagg
     5101 cgacctcctc cttgaggtcc ttcttgacga ccttgcagcc gagctccttc tccgcgacat
     5161 acccgatgac cgcggcgttg gcctcgtagc cgacccaggg gttgatcgcg atgttgaagg
     5221 tgccgcaggc gaccttgcca ccgctcgagg agccggcggc cgcgggggtc tcgccgacct
     5281 tggcgccgcc gcatcccgcg agggtgagcg ccgccgcgga ggccaggaca gcggttcgcc
     5341 gccacccccg cacgtgcttc gagaactgct tcatggcgtg tgaccttccc gttgaagtgc
     5401 tcccccgcgg tgtcgcgggg gacatggatg aggccggccg cgtggcgccg cgcgcggtgg
     5461 cggagaacga ccagggcggg gtatgacgca ccggtcaccc cctcggccgg ctggggtcga
     5521 cccggcgggc cgccgactgg gtgatccggt cgagcatgat gccgagcagc gagatggcca
     5581 ccccggcggc gaggcccttg ccgtagagct ggccctggac cagcccggcg acgacgtcgt
     5641 acccgagggc gccggctccg acgagaccgc cgacgacgac catggccagg acgaagatga
     5701 tcccctggtt ggccccgagc gcgagggcct tggccgccat cgggagctgg acgaggcgga
     5761 tcgtctgcca cgccgtcgcg ccggtcgagg ccgcggcctc catcgtcgtg gccgacaggc
     5821 cacgcacacc gtcggcgatg atcttgatcg aggcgggcgc ggcgtagacg agcgccgcgg
     5881 cgatcgcggt gaaccgcgag gcggcgaaca gggcgaggaa cggcacgagg tagacgaagg
     5941 ccggcagggt ctgaccggcg tcgaggatcg gccggatcgc ctggtccaca cgtctggagc
     6001 gccccatcca cacgccgacg acgacaccga aggccatgac catgatggtg gccagcaggg
     6061 tcgaggcgag ggtgaccatc gcgtcgttcc acaggccgag accgacgatg agcagcaggc
     6121 agatgctggt ggtgacgacg gcgcgccgcc cgccgagcac gccggcgaga cacaccagtg
     6181 ccacgagggc cagccaccag ggcgactcgc tcaggagggc ctgcagcggg tcgagcaggt
     6241 aggccgtgac gatgtccttg aaggcgttgg tcaccggggc gaaggtgtcc tggacccact
     6301 cggtcgcgtt ctcgctccac gtccggatcg tcggcccgag ggtcggttcg gcggggaact
     6361 cggccgccca cacgaacgtg ttggacaggt atgccgccac ggccgtgagc gccgcaccca
     6421 caccgacgac gacgcgccgc aggtgcaggt cccgaccggt cttgggcacg cgctgagccc
     6481 gcacgctcgc cgccgtcgtc acccggtcga ggacgatcgc catgacgacg atcgcgaggc
     6541 cgccgttgac cgactggccg acgtcgaggg actgcagcgc cttgatgacg accttgccca
     6601 gacccggcgc ggcgatgaga gccgtgatcg tggccatcga cagcgccgcc atgatcgtct
     6661 ggttcgcgcc cagcacgatc gtgcgcttgg ccatcgggag catgacctgg gtcagcgact
     6721 gccacgtcgt cgcgccgagg gagtcggtcg cctcgagcgt ggtcgccggc accgtgcgga
     6781 tgccatgggc ggtgagccgg atgaccggcg gcgtggcata gatcagtgtc gcgatcgccg
     6841 ccgcggcggg accgatgagg aagaacaggg tcagcggcgc gaggtagcag
//
LOCUS       DATAIX010000676         2086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_257768, whole genome shotgun sequence.
ACCESSION   DATAIX010000676 DATAIX010000000
VERSION     DATAIX010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257768"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VHM65_09945"
     CDS             <1..339
                     /locus_tag="VHM65_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706069.1"
                     /translation="EYDVVVEVTVDNGPDNWRGRVGLVTQLVPRAGFDPAHTLALVCG
                     PEVMMRSVAAALRDRGVAAARVRLSMERTMKCGIGLCGHCQLRELFICVDGPVLDYER
                     LAPLMLRWEL"
     gene            341..1180
                     /locus_tag="VHM65_09950"
     CDS             341..1180
                     /locus_tag="VHM65_09950"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706070.1"
                     /translation="MTSTPATTSTPATASAPALPTLAVWKFASCDGCQLTLLNCEDEL
                     LSIAEAVQIAYFPEATRAVVEGPYDVSLVEGSVTTPEDAERIHEIRAMSGLLVTIGAC
                     ATAGGIQALRNFADVAAYRSIVYAHPEYLHTLDRSTPISAHVTVDGELRGCPIDKGQL
                     VELLSAALQRRKPRLPTYSVCVECKGRGVVCVAVAHGLPCLGPVTQAGCGALCPSVTR
                     GCYGCFGPQDSTNAAGMSERLLGLGMSTAEAVRVYRTFNAGAEAFRAESERLASTIEA
                     VAP"
     gene            1177..>2086
                     /locus_tag="VHM65_09955"
     CDS             1177..>2086
                     /locus_tag="VHM65_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEX2706071.1"
                     /translation="MTHQLKDGGQVLQVGMLARVEGEGGMHLEIRDGVVQSVRLNIFE
                     PPRFYEAFLRGRAWTEPPDITARICGICPVAYQTAACWAIEGICGVVVPDAIRVMRRL
                     LYCGEWIESHALHIYLLHAPDFLGYDSAIAMAADHRGVVERGLRLKKAGNELMTLVGG
                     RSVHPVNVKIGGFYRMPTVGQLRALRPTVERGLEDALETVRLVAAFDFPEMEQPYEYV
                     ALRSPHGYAIGSGDVVTSSGRSFAVDDFPEHITEHQVPHSTALHATLDGTPDGAPYVV
                     GPLARYSLNFAQLTPTCQDAARAAGLG"
BASE COUNT          291 a          739 c          740 g          316 t
ORIGIN      
        1 gagtacgacg tcgtcgtcga ggtcaccgtc gacaacggcc ccgacaactg gcgcggccgg
       61 gtcggcctgg tgacccagct cgtgccccgg gccggcttcg acccggcgca cacgctggcg
      121 ctcgtctgcg ggcccgaggt gatgatgcgc tcggtcgcgg ccgccctgcg ggaccggggc
      181 gtggcggccg cgcgggtgcg cctgtcgatg gagcggacca tgaagtgcgg catcggtctg
      241 tgtggtcact gccagctgcg tgagctgttc atctgcgtcg acgggcccgt gctcgactat
      301 gagcggctcg ccccgctcat gctgcgatgg gagctctgag atgaccagca ccccggcgac
      361 gaccagcacc ccggcgacgg ccagcgcccc ggccctgccc acgctcgccg tctggaagtt
      421 cgcctcgtgc gacgggtgcc agctcacgct gctcaactgc gaggacgagc tgctctcgat
      481 cgccgaggcc gtccagatcg cgtacttccc ggaggcgaca cgggcggtcg tcgagggccc
      541 gtatgacgtc tcgctcgtgg aaggctcggt caccacgccc gaggacgccg agcggatcca
      601 cgagatccgg gcgatgtcag ggctgctggt gacgatcggc gcctgcgcga cggccggggg
      661 catccaggca ctgcgcaact tcgccgatgt ggcggcctac cgttcgatcg tctacgcgca
      721 cccggagtat ctgcacaccc ttgaccggtc caccccgatc tcggctcatg tcaccgtcga
      781 cggcgagctt cgcggctgcc cgatcgacaa gggccagctc gtcgagctgc tctccgccgc
      841 tctgcaacgg cgcaagccgc gcctcccgac atacagcgtc tgcgtggagt gcaaaggccg
      901 tggcgtggtg tgcgtcgcgg tcgcccacgg cctgccctgc ctcgggccgg tgacgcaggc
      961 gggatgtggc gccctgtgtc cctcggtgac gcgggggtgc tacgggtgct tcgggccaca
     1021 ggacagcacc aacgccgccg ggatgagcga gcggctgctg ggtctcggga tgagcacggc
     1081 cgaggcggtg cgggtctacc gcacgttcaa cgccggggct gaggcgttcc gggccgagag
     1141 cgagcggctc gcgagcacga tcgaggcggt ggcgccatga cccaccagct caaggacggc
     1201 ggccaggtgc tccaggtggg catgctggcc cgggtcgagg gcgaaggcgg gatgcacctc
     1261 gagatccggg acggcgttgt ccagtcggtg cggctcaaca tcttcgagcc cccgcggttc
     1321 tacgaggcgt tcctgcgcgg ccgcgcctgg accgagccgc cggacatcac cgcccgcatc
     1381 tgcggcatct gcccggtggc ctaccagacc gcggcctgct gggcgatcga ggggatctgc
     1441 ggagtcgtcg tgcccgacgc catcagggtc atgcgcaggc tgctctactg cggcgagtgg
     1501 atcgagagcc acgcgctgca catctacctg ctccatgcgc cggacttcct cggctatgac
     1561 agcgccatcg cgatggccgc cgaccaccgc ggggtcgtcg agcgcggcct gcggctcaag
     1621 aaggccggca acgagctgat gaccctggtg ggcgggcgtt ccgtgcaccc ggtcaacgtc
     1681 aagatcggcg gcttctaccg gatgccgacg gtgggccagc tgcgcgcgct cagacccacc
     1741 gtggagcgtg gcctcgagga cgcgctcgag acggtgcgac tcgtcgcggc gttcgacttc
     1801 cccgagatgg agcagcccta cgagtatgtc gcgctccgct cacctcacgg gtatgccatc
     1861 gggtccgggg acgtcgtcac ctcctcgggg cgctcgttcg cggtcgacga cttccccgag
     1921 cacatcaccg agcaccaggt cccgcactcc accgcactgc acgcgaccct cgacgggacg
     1981 cccgacgggg cgccatacgt cgtcggtccg ctggcgcgct acagcctcaa cttcgcccag
     2041 ctgacgccga cctgccagga tgctgcccgg gcggccgggc tggggc
//
LOCUS       DATAIX010000677         2507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_293749, whole genome shotgun sequence.
ACCESSION   DATAIX010000677 DATAIX010000000
VERSION     DATAIX010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293749"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="VHM65_09960"
     CDS             <1..1082
                     /locus_tag="VHM65_09960"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2706072.1"
                     /translation="TTPSGSPVLPVIDPDALEKFTHSVIGDLGAAVSGVLIHIGDRLG
                     LYRALGDSAPVTSVALSARTGLAERYVREWLHNQAAAGWVSYDSNAQTFTLPPEHALL
                     VSDPNAPTFLLGGFDIAASIWGDEHLLTDAFRSGAGIGWHEHDERLFEGTERFFRPGY
                     QANLVSTWLPALNGVVERLEAGIDVADVGCGFGASTIVMARAFPQSTFVGYDYHDASI
                     VAARRRAAEAGLSNVTFEVDTAKAVPGQFDLVCLFDCLHDMGDPEGAACRLRSALRPG
                     GSLLLVEPAGADRPEDNHNPLGRLFYGCSTALCVPSSLAQEGGLGLGNQAGQPRMNEI
                     LTQAGFSSIRVVDRSPINVVMEVRP"
     gene            1279..2415
                     /locus_tag="VHM65_09965"
     CDS             1279..2415
                     /locus_tag="VHM65_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX2706073.1"
                     /translation="MDASRLTDRSLDELVEAAVARGRHSVRGRVARVSGKSWHIGQCA
                     VAAGLAWFVAKDLFHHQVPFFAPIVAVVCLGTTYGQRLRRVVEVTVGVALGVFIADLF
                     VKVVGGGAWQITVIVAAAMAAALFLDAGGFLVTQAALQGIFVATLLPRDEAVFTRWTD
                     ALIGGAVALLAAAVVPQAPLRQPRVQAAVVVRAIACLLREAAAAVGQGDVGRAAQALA
                     DARATEPLIRELHGAADEGLSVLASSPLHRHHAVQVRKVADLIDPIDRALRSTRVLIR
                     RVVVATRSDGPVPASYVAVLTDLAAATDILARALGENASPEVGRSELVRVGERTAGLV
                     RTSYLSTEVLLAQLRSTVIDLLQVTGLSVDEALSVVPPVVDRPT"
BASE COUNT          317 a          937 c          838 g          415 t
ORIGIN      
        1 cgaccacacc gtccggcagt cccgtgttgc cggtcatcga ccccgacgcg ctcgagaagt
       61 tcacccacag cgtgatcggg gacctcggcg cggctgtctc gggcgtgctc atccacattg
      121 gcgaccgcct cggcctctat cgcgctctcg gtgactcggc tccggtgacc tccgtcgcgc
      181 tctcggcccg taccggactg gcggagcgct atgtccgcga gtggctgcac aaccaggcgg
      241 ccgcgggttg ggtcagctac gactcgaatg cccagacctt cacgctgcca cccgagcacg
      301 ccctgctcgt gtccgacccg aacgcgccga ccttcctcct cggcgggttc gacatcgccg
      361 cgtccatctg gggtgacgag cacctcctca ccgacgcctt ccgctccggg gccggcatcg
      421 gctggcacga gcacgacgag cggctcttcg aggggaccga gcggttcttc cgccccggct
      481 accaagccaa cctcgtcagc acctggctac ctgccctcaa tggcgtcgtt gagcgcctcg
      541 aggccgggat cgacgtcgcc gacgtcggct gcgggttcgg cgcgtcgacg atcgtcatgg
      601 cccgggcgtt cccgcaaagc accttcgtcg gctacgacta ccacgacgcc tcgatcgtgg
      661 ctgctcggcg ccgagccgcc gaggcgggcc tgagcaacgt gaccttcgag gtcgacaccg
      721 ccaaggctgt gcccggtcag ttcgatctcg tctgcctctt cgactgcctg cacgatatgg
      781 gcgaccccga gggcgcagca tgccggctcc gctcagcgtt gcggccaggt ggctccctcc
      841 ttctggtaga gccggctggc gcagaccgtc ctgaggacaa ccacaacccc ttggggcgcc
      901 tgttctacgg ctgctcgaca gcgctgtgcg tgccaagctc gctggctcag gaaggcggac
      961 tgggacttgg caaccaggcc ggacagccgc gcatgaacga gatcctcacc caggcaggct
     1021 tctcctccat ccgggtcgtc gaccgctcac cgatcaacgt cgtcatggaa gtgcgcccat
     1081 agcaaggaag gcaaccaggt cctgcggatg atcgagcagc tccgtgagca cggccttgcc
     1141 ggtcatcatc atgaacggtg ccgcggtcct cgaccccgtc gacagcgtca ccggtccggt
     1201 gcgggcctag cggtccgggc ccttccccag gccgatgagg gtgacccatc cgcgggcctg
     1261 ttctggcacc atcgtggggt ggacgcctcg cgcttgaccg accggagcct cgacgagctc
     1321 gtcgaggcag cggtcgcccg cggccggcac tccgtccgcg gacgcgtggc gcgagtgtcg
     1381 ggcaagtcct ggcacatcgg ccagtgtgcc gtcgcggccg gcctggcctg gttcgtggcc
     1441 aaggacctgt tccaccatca ggtgccgttc ttcgctccga tcgtcgccgt cgtctgcctc
     1501 gggaccacct acgggcagcg gctgcgccgg gtcgtcgagg tcaccgtggg cgtcgcgctc
     1561 ggggtcttca tcgcggacct gttcgtcaag gtcgtcggag ggggcgcctg gcagatcacg
     1621 gtcatcgtgg ccgccgcgat ggccgctgcc ctcttcctcg acgccggagg gttcctcgtg
     1681 acccaggccg ccctccaggg gatcttcgtc gccaccctgc tgccgcggga cgaggcggtc
     1741 ttcacccggt ggaccgacgc cctgatcggt ggtgcggtcg ccctcctggc ggcggcagtc
     1801 gtcccgcagg cgccgctgcg ccagccccgg gtccaggccg cagtggtggt ccgtgccatc
     1861 gcctgcctgc tccgtgaggc ggccgcggcc gtcggccagg gggacgtcgg gcgggccgcc
     1921 caggccctcg cggacgcccg ggcgaccgag ccgctgatcc gcgagctgca cggcgcggcg
     1981 gacgagggtc tctcggtgct ggcctcctcg ccgctgcacc gccaccatgc ggtccaggtc
     2041 cgcaaggtcg ccgacctcat cgacccgatc gaccgcgccc tgcgcagcac ccgggtcctc
     2101 atccggcggg tggtcgtcgc cacgcgctcc gatggtccgg tccctgcctc gtatgtcgcc
     2161 gtgctcaccg acctggccgc agcgaccgac atcctggccc gggccctggg cgagaacgcc
     2221 tccccggagg tcggccggtc cgagctggtg cgggtcggtg agcgaaccgc gggtctggtg
     2281 cgcacgtcat acctctcgac cgaggttctc ctcgcgcagc tgcggtcgac cgtcatcgac
     2341 ctgcttcagg tcaccggcct gtctgtcgac gaggcactgt ccgtggtgcc acccgtcgtc
     2401 gaccgcccga cgtaggcccg ccgtatcacc cgctcacggc caggcctctc acctggcatg
     2461 ggccgacggg tctggcgcgt ggtcgccagt cacgggttgg ccgccgt
//
LOCUS       DATAIX010000678         2046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_203675, whole genome shotgun sequence.
ACCESSION   DATAIX010000678 DATAIX010000000
VERSION     DATAIX010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203675"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..1019
                     /gene="rpe"
                     /locus_tag="VHM65_09970"
     CDS             312..1019
                     /gene="rpe"
                     /locus_tag="VHM65_09970"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX2706074.1"
                     /translation="MAPHAPLIVPSVLPADAARLGAEVEALCAAGVDRIQWDVMDGVF
                     VPNLTFGPDVISAARRHSTVGFEAHLMVVEPDELLGRYVDAGCELVIVHAEATTHLHR
                     TLHRIRELGARSGVALNPHTPVSVVENVLDETDMVLAMTVNPGFGGQAYIPAVEAKVR
                     RLRAMVEESGLSIEIEVDGGITNTTIGGAAAAGADVFISGSWLYSFPAGKTAGVQLLR
                     TLARAAMDSSPVDGRVA"
     gene            1021..>2046
                     /locus_tag="VHM65_09975"
     CDS             1021..>2046
                     /locus_tag="VHM65_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX2706075.1"
                     /translation="MTSGITTDIATATVGMVAGAPGTDALAVSTLRFLAADMVEEAQS
                     GHPGLPMGAAPMAWVLFSRHLRHDPTDSHWPDRDRFVLSAGHGSALLYGLLHMSGYDL
                     PVDELRRFRQLGSRTPGHPEFGHTDGVETTTGPLGQGLAMAVGMALAERMQAARFPEI
                     TDHRTYALVGDGCLMEGISQEAISLAGHLGLGRLTVLWDDNRITIDGPAAQSTSDDNQ
                     ARFAASGWHVQSVEDGTALDEIDAALTAAKADPRPSLVAVRTVIGHGAPGIEGTPAAH
                     GAPLGATVLAEAKAAVGWTYPPFTVPDAVADRCAQLADEGRTAHETWSERLARFTEVD
                     PARAGSTS"
BASE COUNT          303 a          746 c          715 g          282 t
ORIGIN      
        1 gggccatctc atcgacgacc cgccgcgcct ccgcgaacga ggtggctgtc cccacgacga
       61 ccatctggtc gtcgaagtga gccagcatgg ccgtgagccc gtgcaggacc atctcgtggt
      121 cgtcgacgag cacgaggcga agcacactgc gaccagcatc gggcacagcc tcaccataag
      181 ggcgaggtcg cttcgatgtg cgcggaaggc gaatcacccc catgaccacc cctgggggtg
      241 agccacgtgc tacccggttc gcggttctct tgcccggtca gcgaggacga ctcgtacccg
      301 ggaggcacga catggcaccg cacgcaccgc tcatcgtccc gtctgtccta ccagcagacg
      361 cagcgcgcct cggcgcggag gtcgaggccc tgtgcgccgc cggcgtcgac cgcatccagt
      421 gggacgtcat ggacggtgtc ttcgtcccga acctcacctt cggtccggac gtcatctccg
      481 cggcgcggcg acacagcacc gtcggcttcg aggcccacct catggtggtc gagccggacg
      541 agctgctggg ccgctacgtc gacgccggct gtgagctggt gatcgtgcat gcggaggcca
      601 ccacccacct gcaccggacc ctgcaccgga tccgcgagct gggtgcgcgt tccggagtcg
      661 cgctcaaccc gcacacgccc gtctcggtcg tggagaacgt gctggacgag accgacatgg
      721 tgctcgcgat gactgtcaac cccggcttcg gcgggcaggc ctacatcccg gcggtcgagg
      781 cgaaggtccg acggctgcgt gccatggtcg aggagtccgg cctgtcgatc gagatcgagg
      841 tcgacggcgg gatcacgaac acgacgatcg gcggcgcggc cgcggccggc gccgacgtgt
      901 tcatctccgg gagctggttg tactccttcc ccgctgggaa gaccgccggg gtgcagctgc
      961 tgcgcaccct cgcgagggcc gccatggact cctcgcccgt cgacggaagg gtggcctgag
     1021 atgacctccg ggatcacgac cgacatcgcc accgccaccg tcggcatggt ggccggggca
     1081 cccgggaccg acgccctggc cgtctccacc ctgcggttcc tcgccgccga catggtcgag
     1141 gaggcgcagt ccgggcaccc gggcctgccg atgggtgccg cgccgatggc ctgggtgctg
     1201 ttctcccggc acctgcgcca cgacccgacc gactcgcact ggccggaccg cgaccggttc
     1261 gtgctgagcg ccgggcacgg ctcggcgctg ctctacgggc tgctgcacat gtccggctac
     1321 gacctgccgg tcgacgagct gcgacggttc cgccagctcg gctcgcggac gcccggtcac
     1381 cccgagttcg gccacacgga cggggtcgag accaccaccg gaccgctcgg ccagggcctt
     1441 gcgatggcgg tgggcatggc gctcgccgag cgcatgcagg ccgcgcgctt cccggagatc
     1501 accgatcacc gcacctacgc gctggtcggc gacggctgcc tcatggaggg gatcagccag
     1561 gaggcgatct ccctggcggg gcacctgggc ctgggacggc tcaccgtcct gtgggacgac
     1621 aaccggatca ccatcgacgg cccggccgcg cagtcgacct ccgacgacaa ccaggcccgg
     1681 ttcgctgcct cgggctggca cgtgcagagc gtcgaggacg ggaccgccct cgacgagatc
     1741 gatgctgcgc tcacagcggc caaggccgac ccgcgcccca gcctcgtcgc ggtgcggacc
     1801 gtcatcggtc acggcgcacc cgggatcgag ggcaccccgg cggcgcacgg agcgccgctc
     1861 ggcgcgacgg tgctggctga ggccaaggca gcagtcggct ggacgtaccc cccgttcacc
     1921 gtgcccgacg ccgtggccga ccgttgcgcg cagctggcgg acgaggggcg cacggcgcac
     1981 gagacatggt cggagcgact ggcgaggttc acggaggtcg acccggcacg tgccgggtcg
     2041 acctcc
//
LOCUS       DATAIX010000679         3192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_233816, whole genome shotgun sequence.
ACCESSION   DATAIX010000679 DATAIX010000000
VERSION     DATAIX010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233816"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VHM65_09980"
     CDS             complement(<1..996)
                     /locus_tag="VHM65_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEX2706076.1"
                     /translation="MRRNKVISADAAARVVFDEDTVATGGFVGVGFPEELALALERRY
                     VETGSPRGLTLVFAAGQGDGRSKGLNHFAHEGMLHRVIGGHWGRAPAVGALALENKVE
                     GYCWPQGVISHLFRDIAASRPGAVTRVGLGTFVDPRHGGGRMNEVTSEQLVELIQLRG
                     EEYLFFPAFPIHVALLRGTTADEEGNVTMEREALTLEALSLAQAAKNSGGVVIVQVER
                     VTTQHVLSPREVKIPGILVDAVVVAEPANHPQTFGESYNPAYTGEIRVPTSSLVPMAL
                     DVRKVIARRAAMFLKMNAVVNLGIGIPEGVASVAGEEHVLDLITLTVEPGGIGGIP"
     gene            1320..2231
                     /locus_tag="VHM65_09985"
     CDS             1320..2231
                     /locus_tag="VHM65_09985"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX2706077.1"
                     /translation="MQLRAVPNRFVRPILLANLLGEVTIVVTGGVVRLTGSGLGCPTW
                     PECVPGSFTPVEEQAEGFHKLVEFGNRTLTGVVGLLALAALWAVWTRYPGRRRLHLLA
                     GGVLAGVLVQAVVGGITVRTGLNPVTVAFHFLVSMVLVALSTMLVAGVHGRQGDDEAD
                     GPGAVLVPALVHRVALGTAAVGGLVLVLGTLVTGSGPHSGDADEPARFGFDPRFVSWM
                     HADAVMLFAGLVIATGLALHVAASPARTRVLWRTVLLVTLAQGLIGYVQYFTQLPEVL
                     VAVHMLGACLLVVALTAAVLSLREHPV"
     gene            complement(2251..>3192)
                     /locus_tag="VHM65_09990"
     CDS             complement(2251..>3192)
                     /locus_tag="VHM65_09990"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX2706078.1"
                     /translation="PRAGLSAVPGSTGSGWWFTVGQYVALTKPRIIELLLITTVPVMF
                     LAQRGVPSFWLVLATIVGGALSAGSANTVNCYLDRDIDAVMNRTSGRPLVTGTISGRA
                     ALVFGVVLAVASTVWLWAFVNWLSAVLSVAAIAFYVGVYTLLLKRRTAQNIVWGGAAG
                     CMPVLIGWAAVTGSLAWTPVVLFLVVFFWTPPHYWPLSMKFRHDYEAARVPMLPVIEH
                     VAVVARQIVRYSWAMVLASLALVPVASMGWVYLGGAVLSGTVFLVEAHRLLRLAKAGA
                     PESVLKPMRLFHYSITYLTLLFVVVALDPLVHLPILR"
BASE COUNT          477 a         1157 c         1099 g          459 t
ORIGIN      
        1 cgggatgccg ccgatcccgc ccggctccac ggtcagcgtg atcaggtcca ggacgtgctc
       61 ctcgccggct accgaggcga cgccctcggg aatgccgatg ccgaggttca ccaccgcgtt
      121 catcttcagg aacatcgcgg cccggcgcgc gatgaccttg cggacgtcga gcgccatcgg
      181 gacaaggctg ctcgtcggga ccctgatctc gccggtgtag gccgggttgt aggactcgcc
      241 gaaggtctgc ggatggttgg cgggctcggc gacgacaacc gcgtcgacga ggattcccgg
      301 gatcttgacc tcgcgcgggc tcagcacatg ctgggtggtc acccgctcga cctggacgat
      361 gacgaccccg cccgagttct tcgccgcctg agccagcgag agggcttcca gcgtcagggc
      421 ctcccgctcc atggtcacgt tgccctcctc gtcggccgtg gtcccgcgta gcagggcgac
      481 gtggatcggg aaggccggga agaacaggta ctcctcgccg cggagctgga tcagctcgac
      541 cagctgctcc gaggtgacct cgttcatccg ccccccaccg tggcgcgggt cgacgaaggt
      601 gccgaggccc actctggtca ccgccccagg tcgtgacgcc gcgatgtcgc gaaacaggtg
      661 agagatcacg ccctgcggcc agcagtaccc ctcgaccttg ttctcgagcg cgagcgcgcc
      721 gacggcaggt gcgcgccccc aatgcccgcc gatgacccgg tggagcatcc cctcatgggc
      781 gaagtggttc aggcccttcg accggccgtc accttgccct gccgcgaaga ccagggtcag
      841 cccacggggt gaccctgtct ccacgtatcg ccgctcgagg gcgagcgcga gctcctcggg
      901 gaacccgacc ccgacgaacc cacccgtggc caccgtgtcc tcgtcgaaga ccacccgcgc
      961 cgccgcgtcc gccgagatca ccttgttgcg acgcatggac gacctcttcc ctgccggtgg
     1021 accaacggac cccagccccg gcaacctcac atcccaggca gatgtgtcaa ggcggcgata
     1081 gcggcatggt aacggcccgc ggcccgagct gctcgatcga ccccgcgcgg gtctccggcc
     1141 ggggtcccat gcggcgacgg ttccgctccg tccccgtcga tggtccccgc agtgccagcc
     1201 cggcgaggtg gcgtggaagc gggcggatcg gcatgcaggt ctgccacccg gccaaccagc
     1261 tgggcaggca cccagtatca acgtcaagtc ggcggaccca ggatggctag gctgtcgtcg
     1321 tgcagcttcg cgccgtcccg aaccgtttcg tccgcccgat cctgctcgcc aacctgctcg
     1381 gtgaggtgac catcgtcgtc accggcggtg tcgtccgcct gaccgggagc gggctcggct
     1441 gcccgacctg gcccgagtgc gtccccgggt cgttcacgcc ggtcgaggag caggccgagg
     1501 ggttccacaa gctcgtcgag ttcggcaacc gcaccctgac cggcgtcgtc gggctgctcg
     1561 ccctggcggc cctctgggcg gtctggaccc gataccccgg ccggcgccgg ctccacctgc
     1621 tggccggcgg ggtgctcgcg ggcgtcctcg tccaggcggt cgtcggcggc atcaccgtgc
     1681 gcaccgggct caacccggtg acggtggcct tccacttcct cgtctcgatg gtcctcgtcg
     1741 cgctctcgac gatgctcgtc gccggggtcc acggccgtca gggcgacgac gaggccgacg
     1801 gtccgggggc agtgctggtc cctgcgctgg tccaccgggt ggcgctcggc actgcggcgg
     1861 tcgggggcct cgtcctcgtt ctcggcacgc tcgtcaccgg ttcggggccg cactcaggag
     1921 atgccgacga gccggcccgg ttcgggttcg acccgcggtt cgtgtcgtgg atgcatgccg
     1981 acgccgtcat gctcttcgcc ggcctcgtca tcgcgaccgg gctcgccctg cacgtggccg
     2041 cctcgccggc ccggacccgc gtgctctggc gcaccgtcct gctcgtcacc ctggcccagg
     2101 ggctcatcgg ctacgtccag tacttcaccc agctccccga ggtgctcgtc gccgtccaca
     2161 tgctcggcgc ctgcctactc gtcgtcgccc tcaccgcggc cgtcctcagc ctgcgtgagc
     2221 acccggtctg accagaccct gcggggcccg tcagcgcagg atcggcaggt ggaccagcgg
     2281 gtcgagggcg acgacgacga agaggagcgt gagatatgtg atcgagtagt ggaagagccg
     2341 catcggcttg agcaccgact ccggcgctcc cgccttggcc aggcgcagca gccggtgcgc
     2401 ctcgacgagg aagacggtcc cgctgagcac cgcgccgccg aggtagaccc agcccatcga
     2461 cgccaccggc acgagcgcga gcgaggcgag caccatggcc caggagtagc ggacgatctg
     2521 gcgggcgacg acggcgacgt gctcgatgac gggcagcatc ggcacccggg ccgcctcgta
     2581 gtcgtggcgg aacttcatcg acagcggcca gtagtgcggc ggggtccaga agaagacgac
     2641 gaggaagagc acgaccgggg tccaggcgag tgagccggtc acggccgccc agccgatgag
     2701 cacgggcata cagcccgccg caccgcccca cacgatgttc tgcgccgtgc gccgcttgag
     2761 cagcagggtg tagaccccga cgtagaaggc gatggctgcg accgacagca cggccgagag
     2821 ccagttgacg aaggcccaga gccagaccgt cgacgccacc gcgaggacga ccccgaagac
     2881 gagcgccgcc cggcccgaga tcgtgcccgt cacgagtggc cggccgctcg tgcggttcat
     2941 gaccgcgtcg atgtcacggt cgaggtagca gttgaccgtg ttggccgacc cggccgacag
     3001 ggccccgccg acgatggtgg cgaggacgag ccagaacgac gggaccccgc gctgggcgag
     3061 gaacatcacc ggcacggtcg tgatgagcaa gagctcgatg atccggggct tggtcagggc
     3121 gacgtactgg cccacggtga accaccaccc cgacccggtc gatcccggga cggctgagag
     3181 cccggcacga gg
//
LOCUS       DATAIX010000680         2602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_233849, whole genome shotgun sequence.
ACCESSION   DATAIX010000680 DATAIX010000000
VERSION     DATAIX010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233849"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(534..977)
                     /locus_tag="VHM65_09995"
     CDS             complement(534..977)
                     /locus_tag="VHM65_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2706079.1"
                     /translation="MITHILLAVDDSRDALAATRLAVELSRGLGARLRAVHVSADHHV
                     EAAIEAATGTPVGVRRGDAEALLLARVVALAEAAGVAAETVLLRGRVEAALIQAARDW
                     GADLVVIGKSDRGAVGDPYVGSLTRHVLEFAEQPVLVVPVPVRHR"
     gene            complement(974..1603)
                     /locus_tag="VHM65_10000"
     CDS             complement(974..1603)
                     /locus_tag="VHM65_10000"
                     /inference="COORDINATES: protein motif:HMM:NF013935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEX2706080.1"
                     /translation="MPSLTAVPPGRAGRSWLRRRLATAERGREQLDRKLRILFPEEQR
                     LRILADRQRTEWVSACAEADRWLLRAALLGGQDGIRDASTLQPVTVEVQWATTMGLSY
                     PSGAELSPARDEVSLPVSNAAIDPAAAAVRAALQAGVRTAAAEEAVRRLDAEIAVTRR
                     RLRALEKRWLPRLHAELVGLELTLEQTEQEDTLRLRRAAATTGERTARP"
     gene            complement(1608..>2602)
                     /locus_tag="VHM65_10005"
     CDS             complement(1608..>2602)
                     /locus_tag="VHM65_10005"
                     /inference="COORDINATES: protein motif:HMM:NF003235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEX2706081.1"
                     /translation="PVLTGISAIDALTTLVRGQKLPIFSVAGLPHLELAAQIAAQSTA
                     GSEPFSVVFAAMGLTHADTDAIRDTLDDRLAAGELLLLLNTADDPVIERILTPRIALT
                     VAEHLAFELDRHVLVVLADMTSYAEALREVSSARGELPGRRAYPGYLYSDLATLYERC
                     GRIRGRSGSVTVLPVLTMPAGDITHPVPDLTGYITEGQVVLSPDVHARGIYPPVDSLG
                     SLSRLMRNGAGPGRTREDHLELAAQLLAALARARQVRELAEIVGDSALSATDRLYLDF
                     ATAFERGLIDQQRHEQREMGQTLDRCWDVANVLPRRELTMLSRALLDAHPVRED"
BASE COUNT          347 a          908 c          985 g          362 t
ORIGIN      
        1 ctcttccact cctccactgt cctcgcgatc atcccggggc ggaagagcgc tggaccacct
       61 ccacgtacca ggtctggtgt gaggatccac gccgctcggt tgtcaccgtg ccggacttgg
      121 tggagggcct ggcgcggggg cggcgagatc gtcaacaggc gcaacgctgg cgtcacatct
      181 ggtgatctga ccggcccttg gctcgacgtc gcggcatcgt gtcacgcccg aggccatcta
      241 ttgggcctcg atgcccgtca gccggatacc ggccggcaca tctgggttcg ccctgcccga
      301 tatgacaccg atgcgggcac caagtcacca ggtcaccgga cctccgcgtc gcggggagct
      361 ttcgagtagg tccggccttg gggcatgatt ggacggttcg cgcacctggt tccgctcagg
      421 tgcgggttca tgactggtag ggcgtctttg tcgtcatgaa gcaccacacg aactaccgca
      481 gacggcctct gcgcggcaca tccgtcgatc gaggttctcg acgggcccgc cggtcatcgg
      541 tggcgcaccg gcaccggtac cacgagcacc ggctgctcgg cgaactcgag cacgtggcgg
      601 gtgagcgagc cgacgtaggg gtcaccgacg gccccacggt cggacttccc gatgacgacg
      661 aggtcggcac cccagtcccg ggcggcctgg atcagggcag cttccacgcg gcccctgagc
      721 aggacggtct cggcggcgac gcccgcggcc tcggcgagtg cgacgacgcg tgcgagcagc
      781 aaggcctccg catcgccgcg ccggaccccg acgggggtcc ccgtggccgc ctcgatcgcc
      841 gcctcgacgt ggtggtcggc gctgacgtgc acggcgcgca gccgagcccc cagcccacgg
      901 gacagctcca cggcaagccg ggtggcggcg agtgcgtccc tggagtcgtc caccgccagc
      961 aggatgtgcg tgatcacggc cgagccgtcc tctcccccgt ggtggcggct gctcgacgca
     1021 gccgcagggt gtcctcctgc tcggtctgct cgagggtcag ctcgaggccg accagctcgg
     1081 cgtgcagccg tgggagccac cgcttctcga gcgcgcgcag ccgtcggcgc gtcaccgcga
     1141 tctcggcgtc gagccgtcgc accgcctcct ccgcggccgc ggtgcgcacc ccggcctgca
     1201 gggcggcacg caccgcggcg gcggcggggt cgatcgcggc gttcgagacc ggcagggaca
     1261 cctcgtcccg ggccggggac agctccgccc ccgaggggta gctcaggccc atcgtcgtcg
     1321 cccactgcac ctcgacggtg accggctgca gcgtgctcgc gtcacggatg ccgtcctgcc
     1381 cgccgaggag ggctgcccgg agcagccacc ggtcggcctc cgcgcaggcg gacacccact
     1441 cggtgcgctg ccggtccgcg aggatgcgca gccgctgctc ctcggggaac aggatgcgca
     1501 gcttccggtc gagctgctcc cggccacgct cggcggtggc cagtcgccgg cgcagccagc
     1561 tgcggccggc acgaccggga gggacggctg tcaggctcgg cagggcgtca gtcctctcgc
     1621 acggggtggg cgtcgaggag cgcccgggag agcatggtca gctcgcggcg cggcaggacg
     1681 ttggcgacgt cccagcaccg gtcgagggtc tggcccatct cgcgctgctc gtgacgctgc
     1741 tggtcgatca gaccccgctc gaaggccgtc gcgaagtcga ggtagagccg gtcggtggcg
     1801 ctcagggccg agtcgccgac gatctcggcg agctcgcgga cctgtcgcgc ccgcgcgagg
     1861 gcggccagca gctgggcggc cagctcgagg tggtcctcgc gggtgcggcc ggggcccgcg
     1921 ccgttgcgca tcagccggga caacgagccc agggagtcga ccggcgggta gatgccgcgc
     1981 gcgtgcacgt cgggcgagag cacgacctgg ccctcggtga tgtagccggt gaggtcgggc
     2041 accgggtggg tgatgtcgcc ggcgggcatg gtgagcacgg gcagcacggt gaccgagccg
     2101 ctccgccccc ggatgcgccc gcagcgttcg tacagcgtgg cgaggtcgct gtagaggtac
     2161 cccgggtagg cccgccggcc gggcagctcc ccgcgggccg aggacacctc gcgcagcgcc
     2221 tcggcgtagc tcgtcatgtc cgccagcacg accagcacgt ggcggtcgag ctcgaaggcg
     2281 aggtgctcgg cgacggtgag ggcgatgcgc ggcgtgagga tgcgctcgat gaccgggtcg
     2341 tccgcggtgt tgagcagcag gagcagctcc cccgcggcga ggcggtcgtc cagcgtgtcg
     2401 cggatggcgt cggtgtcggc atgggtgagg cccatcgccg cgaagacgac gctgaacggc
     2461 tcggagccgg cggtcgactg cgcggcgatc tgggcggcca gctcgaggtg gggcaggccg
     2521 gcgaccgaga agatcggcag cttctgcccg cgcacgagcg tcgtgagcgc gtcgatggcg
     2581 gagattccgg tgaggaccgg gt
//
LOCUS       DATAIX010000681         1800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_294670, whole genome shotgun sequence.
ACCESSION   DATAIX010000681 DATAIX010000000
VERSION     DATAIX010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294670"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..367)
                     /locus_tag="VHM65_10010"
     CDS             complement(2..367)
                     /locus_tag="VHM65_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEX2706082.1"
                     /translation="MKQLRTVVGVLGELLVTAGVVIVLYVVWELGFTAVVETREQHQV
                     VAQLEQELAVPGPATPTTATKPPVGTVFAILRIPRLGGPGWARPVYSGTTRDILAKGL
                     GYYPTTAVPGQPGNTAVVG"
     gene            complement(381..1142)
                     /locus_tag="VHM65_10015"
     CDS             complement(381..1142)
                     /locus_tag="VHM65_10015"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEX2706083.1"
                     /translation="MAGRDRVDRWLFGYRPSRWSLVVPIVFAVAGGLFATSFTTARGT
                     EIRSIGRDLPNQVRDATRTVEEKTRRVAGLERQIDTFTADLVPSDGQLAAIARQSETL
                     ALGAGTRTVKGPGVTVRLDDAPRRLDSLPAGMTADDVVVHQQDVQAVVNALWSGGAEA
                     MMIQDQRVISTSAVRCVGNTLILQGRVYSPPYVIAAIGPREALLASLEASPTVQIYRQ
                     YVDAVGLGYDVTSPGSATFPAYAGTVKLQFARVAR"
     gene            1218..1466
                     /locus_tag="VHM65_10020"
     CDS             1218..1466
                     /locus_tag="VHM65_10020"
                     /inference="COORDINATES: protein motif:HMM:NF018487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HEX2706084.1"
                     /translation="MPESKGRPKPSYTPPPGRSKPAGPSPRWWAPTFVTLLLLGLVWI
                     VTFYVSEQRFPVQALGYWNLGVGFGLMMTGFSMTMNWR"
     gene            complement(1557..>1800)
                     /locus_tag="VHM65_10025"
     CDS             complement(1557..>1800)
                     /locus_tag="VHM65_10025"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX2706085.1"
                     /translation="SSSWVYATLAINAVFGFLYAGISWQAHLGGFVTGLGCAAVMARL
                     RQGTARRWQVPALLAVVILLAGLAWLKYLAVPAQYW"
BASE COUNT          286 a          641 c          618 g          255 t
ORIGIN      
        1 actacccgac gacggcggtg ttgcccggct gccccgggac cgccgtcgtc gggtagtagc
       61 ccagaccctt ggcgaggatg tcgcgcgtcg tccccgagta gaccggccgg gcccagccgg
      121 ggccgccgag cctggggatg cgcaagatgg cgaagaccgt gccgaccggc ggcttcgtcg
      181 cggtcgtcgg cgtggccggt cccgggacgg cgagctcctg ctcgagctgg gcgaccacct
      241 ggtgctgctc ccgggtctcg acgacggccg taaagccgag ctcccacacg acgtagagca
      301 cgatgacgac ccccgcagtg acgagcagct caccgagcac cccgacgacg gtccgcagct
      361 gcttcatcgg gcggccagcc tcatcgggcc acccgggcga actggagctt caccgtgcca
      421 gcgtacgcgg gaaaggtcgc cgaacccggc gaggtcacgt cgtagcccag gcccaccgcg
      481 tcgacatact ggcggtagat ctggaccgta gggctggcct cgagcgaggc caggagcgcc
      541 tcgcgggggc cgatcgccgc gatgacgtag ggcggggagt agacccgccc ttgcaggatc
      601 agcgtgttgc cgacacagcg gacggccgag gtcgagatga cccgctggtc ctggatcatc
      661 atggcctcgg cgcccccgga ccacagggcg ttgacgacgg cctggacgtc ctgctggtgc
      721 accacgacgt cgtcggcggt catgcccgcc gggaggctat cgagccggcg cggtgcgtcg
      781 tcgagccgga ccgtgacccc ggggcccttg actgtgcgcg tgccggcgcc gagcgcgagg
      841 gtctcgctct ggcgggcaat ggcggccagc tggccgtcgg agggcacgag gtccgcggtg
      901 aacgtgtcga tctgacgctc gaggccggcg acccgccggg tcttctcctc gacggtccgg
      961 gtcgcgtccc tgacctggtt cgggaggtcc cggccgatcg accggatctc cgtgccgcgg
     1021 gccgtcgtga agctcgtcgc gaagaggccg ccggccaccg cgaacacgat gggaacgacg
     1081 agggaccacc gcgacggacg gtagccgaac agccaccggt ccacccggtc gcgacctgcc
     1141 acggcgcccc tccctcgcaa ttgcggtcga gcaactactc tggaagccaa ccgtctcaca
     1201 tcgcaaggag cacgcccgtg cccgagtcca aaggccgccc gaagccgagc tacaccccgc
     1261 caccgggccg ctccaagccc gccggcccga gcccgcggtg gtgggcaccg accttcgtca
     1321 ccctgctcct gctcggtctc gtctggatcg tgacattcta cgtctcggag caacgcttcc
     1381 cggtccaggc gctcggctac tggaacctcg gtgtcggctt cggactcatg atgaccgggt
     1441 tctcgatgac gatgaactgg cgctagccgc tgctcgtcag ccgcgctccg gagttatcca
     1501 cagagttgtt atccacagag ttctccacac ctggggataa atgacatccg tgtgattcac
     1561 cagtattggg ccggcactgc gaggtacttc agccacgcca ggccggccag gaggatgacc
     1621 accgcgagca gtgccggcac ctgccagcgc cgggccgtcc cttgacgcag cctggccatc
     1681 accgcggcgc agccgagccc ggtcacgaag ccgccgaggt gcgcctgcca ggagatcccc
     1741 gcgtagagga acccgaagac cgcgttgatc gccagggtgg cgtagaccca gctcgacgag
//
LOCUS       DATAIX010000682         9038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_204598, whole genome shotgun sequence.
ACCESSION   DATAIX010000682 DATAIX010000000
VERSION     DATAIX010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 9038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9038
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204598"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1673)
                     /locus_tag="VHM65_10030"
     CDS             complement(114..1673)
                     /locus_tag="VHM65_10030"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX2706086.1"
                     /translation="MTREAQLRMRLLGPAVGDRLLGWLGPLAAMAVGGLLRFWDLARP
                     HQLVFDETYYVKQGWSMVLYGFEMRNDPVLDAAKQIDQNFTGTNQSVYGTVGDLVVHP
                     PVGKWLIGWGEQIFGVTSSFGWRFAAAVVGTLSILMVGRIGRRLLGSSLLGTVAALLL
                     AFEGHHFVHSRTGLLDVFVMFFALAGFAALLIDRDSSRARLARVLAEPLSAPSALGPW
                     LGLRPWRWVAGVALGLCTGTKWSGLFVLAVFGVMTVLWDAGARRTVGVRRWLRGAAVR
                     DGPLAFVALVVVACLTYVASWTGWFRTSGGYDRHWAVEHPSARFGWVPGALRSLWHYH
                     GEIYQVSRGITSAHDYQSSPWSWLVQGRPTSFFYEGPKRGEMGCTVDLCSKAITSVGT
                     PVIWWAGTIGVVVLLFRWALRRDWRAGAILAGFLGLYVPWFGLGDRTIFTFYAVAFAP
                     FVVLTVTYLCGLAIGPAGASRRRRRLGLGLTGSFVVASVLVFAFFYPIYTAWVVPYDF
                     WRIHMWFPSWI"
     gene            1749..2717
                     /locus_tag="VHM65_10035"
     CDS             1749..2717
                     /locus_tag="VHM65_10035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX2706087.1"
                     /translation="MTNASPAAGGGPGTAPAATRDLTRYAWLAIAAAITTILLKAGAW
                     LLTGSVGLLSDAAESIVNLVAAVVALIALRVAAMPADDNHHFGHTKAEYFSAAIEGVM
                     IFVAAVVILITSVQRFLSPLPLENIGVGLGISAVASVVNGAVAFVLIRAGRAYRSVTL
                     TADGRHLVTDVWTSVGVIVGVLGVAATGWLRLDPIVAFAVGVNIIVTGSRLLSMSING
                     LMDHAMSEEEHATINHVLDGFASDEVVFHALRTRESGHQRFLSVHVLVPGAWSVRQGH
                     DLVSDVEDALSQAYSDLHIETHLEPIEDPRAYGDAPADVRTGITPV"
     gene            complement(2704..3756)
                     /locus_tag="VHM65_10040"
     CDS             complement(2704..3756)
                     /locus_tag="VHM65_10040"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX2706088.1"
                     /translation="MTTTPKTPTPGPGTTGREDLLVVTDLQKHFPIRKGLLQRQVGAV
                     KAVDGVSFTVRTGETLSLVGESGCGKTTTGRMLTRLIEPTGGRVVFEGRDITHISPGK
                     MRPLRRDVQMIFQDPYGSLNPRHTVGTIVGAPYKLQNIKTEHGTKKAVQELLELVGLS
                     PEHYNRYPHEFSGGQRQRIGIARSLALRPKLIVADEPVSALDVSIQAQVINLLEDLQD
                     EFNLTYVTIAHDLSVVRHISDRIAVMYLGKIVEVADRDTLYERPMHPYTVALLSAVPI
                     PDTGRRTKRDRIRLTGDVPSPIDPPSGCRFRTRCWKAQEVCATTEPPLAPLGIDHDVA
                     CHFPENQTAQDAVKPV"
     gene            complement(3753..4778)
                     /locus_tag="VHM65_10045"
     CDS             complement(3753..4778)
                     /locus_tag="VHM65_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2706089.1"
                     /translation="MSAESFLQVRDLRVHFPTDDGVVKSVDGLSFDLDRGQTLGIVGE
                     SGSGKSVTSLSIMGLHKPGTADISGSVRLEDQELIGASPEAVRQLRGQKMAMIFQDPL
                     SAMHPFYTVGHQIIEAYRIHNDVSAKVARAHAVDLLDRVGIPQARNRVDDYPHQFSGG
                     MRQRAMIAMALSCDPQLLIADEPTTALDVTVQAQILDLIRDLQKEFNSAVILITHDLG
                     VVAELADDILVMYAGRCVEYSGADSIFSTPQHPYTWGLLGSMPRLDRARTARLRPIKG
                     QPPSLITVPPGCPFHPRCAYADLTEGRSRTERPAFLEISPHHYIACHLSPEDRNRLWE
                     NEIRPTL"
     gene            complement(4775..5818)
                     /locus_tag="VHM65_10050"
     CDS             complement(4775..5818)
                     /locus_tag="VHM65_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2706090.1"
                     /translation="MAATGTAPQHMVTFLVRRLIGAVVLLFIVSAITFAIFFLVPRLG
                     GATAEDLASRYVGKSAGQEQIHETAVKLGFTDPIWLQYGRFAKGIVAGQDYNYGPETE
                     HCPAPCLGYSFVTQNPVLPEILNRLPVTMSLASGAAVLWLVGGVATGVVSALKRGSRL
                     DRTMMTVALAGVSLPIFFTGLLSLSIFSYGLGWTAPGASNPMEAANPLDWAYRLILPW
                     ITLAFLYAAAYARLTRAGMLETMNEDYIRTARAKGLRERDVVVKHGLRAALTPITTIF
                     GLDLGLLLGGAILTESTFSLPGIGLYARQAITNNDLPKVLGVTLVAAVFIIAANLVVD
                     LLYAVIDPRVRTR"
     gene            complement(5922..7691)
                     /locus_tag="VHM65_10055"
     CDS             complement(5922..7691)
                     /locus_tag="VHM65_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2706091.1"
                     /translation="MRNTSKRGPVLVAGAVLALGLSACSGSSGTSAGTSASGAAGAAN
                     AAVSAVFNPSDTKGGTLRFGMAGTLDSVDPGDMYYGYSWNFVRNYARTLVVFKAAPGK
                     AGNVLVPDLAESLGVPSKDFKTWTYKLKSGLKFEDGTPITSKDVKYAVARSLDKNTFP
                     NGPTYFNNFLADVPAKYTVYPSKSDAGLTAIETPDDRTIIFHLKRSFSGFDYFAMLPQ
                     TAPVPAAKDTGAKYKEKVVSSGPYKFSSYEADKRYVLVRNENYDPATDKDSGRKALPD
                     SLTVELGLNAADVDNRLQAGDLDIDFAGSGVAAESQAKILSNPDLKAKTDSAASARTW
                     YANINSDVPPLDNIECRKAVLYGYDKTGYQRAYGGETGGDIATSLLPPKIPGHREIDL
                     YDAKAKPQGDEAKAKAALAACGKPDGFETVMSYRAERPKEKNMAEAMQQSLAKIGIKL
                     TLKGFPQGDYFKLYAGKPDFAKANGLGILTFGWAADWPDGFGALAQIVDSRTIRPAGN
                     TNLGIKIPAVDALLDKALVTADAPAREKLWGDIDELVMENALVIPGIYAKGLLFRPAT
                     VTNIFINDAFGEYDYTAAGTSKK"
     gene            complement(7739..8728)
                     /locus_tag="VHM65_10060"
     CDS             complement(7739..8728)
                     /locus_tag="VHM65_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2706092.1"
                     /translation="MTVPLDIEPTHPLDTDAVLEGESAIQGRSLSEIAWRRLRRDRVA
                     IAGGVVVVVLIVGAVLAPLVVTLLGHPPDEFHYDQVDASTGLPLHGFGGVSTDFLFGV
                     EPINGRDLFSRVLYGSQTSLLIAFLATLLSVFIGTVMGVVSGFTGGWVDGLISRLMDI
                     FLAFPLLVFALALVGAIPDKAFGLKGDSLRIAILIFIIGFFNWPYIGRIIRGQTLSLR
                     EREFVDAARSLGGRTGYILFRELLPNLVAPILIYATLLIPANILFEAGLSFLGVGVQP
                     PRATWGGMLSDAAGWYQTDVQFMIPPGLAIFVTVLAFNLFGDGLRDALDPRSR"
BASE COUNT         1430 a         2967 c         3290 g         1351 t
ORIGIN      
        1 gtccacgacg ggtcgtaggc atacgtctcg cccgcagcga ccacctcgtg cacgatcggc
       61 cagagggcgc cccagtcggc gtcgacgtag tcccggatca gcatggcccc accctagatc
      121 cagctgggga accacatgtg gatccgccag aagtcatagg gcacgaccca cgccgtgtag
      181 atcgggtaga agaacgcgaa gacgaggacc gaggccacga cgaacgagcc cgtgagcccc
      241 agcccgagcc gtcgccgccg ccgtgaggcc ccggccggac cgatcgccag gccgcacagg
      301 taggtcaccg tgagcacgac gaacggcgcg aaggcgacgg cgtagaacgt gaagatggtc
      361 cggtccccga gaccgaacca cggcacatag aggccgagga acccggcgag tatggcgccc
      421 gcccgccagt cccggcgcag cgcccaccgg aagagcagga cgacgacccc gatcgtgccc
      481 gcccaccaga tgaccggggt gccgaccgac gtgatcgcct tggagcacag gtcgacggtg
      541 cagcccatct ccccgcgctt gggcccctcg tagaagaacg acgtcggccg cccctgaacc
      601 agccacgacc acggcgagct ctggtagtcg tgcgccgagg tgatcccccg gctgacctgg
      661 tagatctcgc cgtgatagtg ccacagcgac cgcagcgcgc cgggaaccca gccgaaccgc
      721 gcggaggggt gctccaccgc ccagtgccgg tcgtagccgc cggaggtgcg gaaccacccg
      781 gtccacgacg cgacatacgt caggcacgcc acgaccacca gggcgacgaa cgcgagcggc
      841 ccgtctcgca ccgccgcgcc acgcagccac cgccggaccc cgaccgtccg gcgggcgccc
      901 gcgtcccaca gcacggtcat caccccgaag accgcgagga cgaacagccc ggaccacttc
      961 gtccccgtgc agagcccgag cgcgaccccg gccacccacc gccacggccg cagacccagc
     1021 cacggcccga gcgccgatgg cgcggagagg ggctcagcca ggacgcgggc cagccgcgct
     1081 cgcgaggagt cccggtcgat gagcagcgcg gcgaaccccg cgagggcgaa gaacatgacg
     1141 aagacatcga gcagcccggt ccgggagtgc acgaagtgat ggccctcgaa ggccagcagc
     1201 agcgccgcca ccgtcccgag cagcgaggac cccaggagcc gccgcccgat gcggcccacc
     1261 atgaggatcg acagcgtccc cacgaccgcg gcggcgaagc gccagccgaa cgagctggtg
     1321 accccgaaga tctgctcgcc ccacccgatg agccacttgc cgacgggagg gtgcacgacg
     1381 aggtcgccga ccgtgccgta gacggactgg ttggtccccg tgaagttctg gtcgatctgc
     1441 ttggccgcgt cgagcacggg gtcgttgcgc atctcgaacc cgtacaggac catcgaccag
     1501 ccctgtttga cgtagtacgt ctcgtcgaac acgagctggt gcggccgcgc gaggtcccag
     1561 aagcgcagca gacccccgac ggccatcgcc gcgagcgggc cgagccagcc gagcagccgg
     1621 tcgccgaccg cgggcccgag caggcgcatg cgcagctgcg cctcccgggt catggacgcc
     1681 atcgtatgca gtggccgggc tggggacgcc cgcggccacc gcgcccgcgc cgggcgataa
     1741 tggctggtat gacgaacgcc tcaccggccg ctggtggtgg gcccgggacc gccccagcgg
     1801 cgacgcggga cctcactcgc tacgcctggc tcgccatcgc cgccgccatc acgacgatct
     1861 tgctcaaggc gggcgcgtgg ctgctcaccg gctcggtcgg gctgctgtcc gacgcggccg
     1921 agtcgatcgt caacctcgtc gccgccgtgg tcgccctcat cgccctgcgg gtcgccgcga
     1981 tgccggccga cgacaaccat cacttcggcc acaccaaggc cgagtacttc tcggccgcca
     2041 tcgagggcgt catgatcttc gtggcggccg tcgtcatcct catcacctcg gtgcagcgct
     2101 tcctgagccc tctgccgttg gagaacatcg gcgtcgggct cgggatctcc gcggtcgcct
     2161 cggtcgtcaa cggagcggtc gccttcgtcc tcatccgggc cgggcgcgcc taccgctcgg
     2221 tcaccctgac cgcggacggg cggcacctcg tcaccgacgt gtggacctcg gtcggggtca
     2281 tcgtcggcgt cctcggggtc gccgcgaccg ggtggctgcg cctcgacccg atcgtcgcct
     2341 tcgcggtcgg cgtcaacatc atcgtgaccg ggtcccggct gctgtccatg tcgatcaacg
     2401 gcctcatgga ccacgcgatg agcgaggagg agcacgcgac catcaaccac gtcctcgacg
     2461 ggttcgcctc cgacgaggtg gtcttccacg ccctgcgcac ccgcgagtcg ggccaccagc
     2521 gcttcctgtc ggtccatgtg ctcgtgccgg gtgcctggtc ggtccggcag ggccacgacc
     2581 tggtgtccga cgtcgaggac gccctgagcc aggcctactc ggacctgcac atcgagaccc
     2641 acctcgaacc gatcgaggac ccacgtgcct acggcgacgc gccggccgac gtgcgcacgg
     2701 gcatcacacc ggtttgaccg cgtcctgggc cgtctggttc tccgggaagt ggcaggcgac
     2761 gtcgtggtcg atcccgagcg gcgccagggg tggctcggtg gtcgcgcaga cctcctgggc
     2821 cttccagcag cgggtccgga agcggcaccc tgacggtggg tcgatcggtg agggcacgtc
     2881 gccggtgagc cggatccggt cacgcttggt ccggcgcccc gtgtccggga tcgggacggc
     2941 cgagagcagg gccaccgtgt acgggtgcat cggccgctcg tagagggtgt cccggtcggc
     3001 gacctcgacg atcttgccga ggtacatcac ggcgatccgg tccgagatgt gccgcacgac
     3061 cgacaggtca tgggcgatcg tgacgtaggt gaggttgaac tcgtcctgca ggtcctcgag
     3121 caggttgatg acctgcgcct ggatcgacac gtccagggcc gacaccggct cgtcggcgac
     3181 gatgagcttg ggccgcagcg cgagcgaccg ggcgatcccg atgcgctggc gctgtccccc
     3241 ggagaactcg tgcggatagc ggttgtagtg ctcgggggag agcccgacca gctcgagcag
     3301 ctcctggacc gccttcttcg tgccgtgctc ggtcttgatg ttctgcagct tgtatggcgc
     3361 cccgacgatc gtcccgacgg tatggcgggg gttcagcgag ccgtacgggt cctggaagat
     3421 catctgcacg tcgcggcgca gcgggcgcat cttgccgggc gagatgtggg tgatgtcgcg
     3481 gccctcgaag acgacccggc cgccggtggg ctcgatgagc cgggtgagca tccggccggt
     3541 cgtcgtcttg ccacagccgg actcgccgac gagggagagg gtctcacccg tgcggaccgt
     3601 gaagctgacg ccgtcgaccg ccttgaccgc gccgacctgg cgctggagca ggcccttgcg
     3661 gatcgggaag tgcttctgca ggtcggtgac gacgagcagg tcctcgcgtc cggtcgtgcc
     3721 cgggcctggt gtcggggtct ttggtgtggt tgtcatagcg tcggccgaat ctcgttctcc
     3781 cacaagcggt tacggtcctc aggggaaagg tggcaggcga tgtagtggtg gggcgagatt
     3841 tccaggaagg ccggacgttc ggtgcggctg cggccctcgg tgaggtcggc atacgcgcag
     3901 cgggggtgga aggggcaccc cggcggcacg gtgatcaggc tcggcggctg gcccttgatg
     3961 gggcgcagcc gcgcggtgcg ggcccggtcc aggcgaggca tcgacccgag cagcccccac
     4021 gtgtagggat gctggggggt cgagaagatc gagtcggcac cggagtactc gacgcaccgg
     4081 ccggcataca tgacgaggat gtcgtcggcg agctcggcga cgacgccgag gtcgtgcgtg
     4141 atgaggatga ccgccgagtt gaactccttc tgcaggtcgc ggatgaggtc gaggatctgc
     4201 gcctggacgg tgacgtccag ggccgtcgtc ggctcgtcgg cgatgagcag ctgcgggtcg
     4261 caggacagcg ccatggcgat catggcccgc tggcgcatcc cgccggagaa ctggtggggg
     4321 tagtcgtcga cccggttcct ggcctggggg atgccgacgc ggtcgagcag gtcgaccgcg
     4381 tgcgcgcgag cgaccttggc cgacacgtcg ttgtggatcc ggtaggcctc gatgatctga
     4441 tggcccaccg tgtagaaggg atgcatcgcc gagagcgggt cctggaagat catcgccatc
     4501 ttctggcccc gcagctggcg gaccgcctcg gggctcgccc cgatgagctc ctggtcctcg
     4561 agccggaccg agccggagat gtcggccgtc ccgggcttgt gcaggcccat gatcgacagg
     4621 ctcgtcacgc tcttgcccga cccggactcg ccgacgatgc cgagtgtctg gccgcggtcg
     4681 aggtcgaagg agagcccgtc gacgctcttg acgaccccgt cgtcggtggg gaagtggacg
     4741 cgcaggtccc ggacctggag gaaggactcg gcgctcacct ggtccgcacc ctcgggtcga
     4801 tgacggcgta gagcaggtcg acgacgaggt tggccgcgat gatgaagacg gctgcgacaa
     4861 gggtgacgcc gaggaccttg ggcaggtcgt tgttcgtgat cgcctggcgc gcgtagaggc
     4921 cgatccccgg cagggagaac gtcgactcgg tgaggatcgc accaccgagg agcagcccga
     4981 ggtcgaggcc gaagatcgtc gtgatcgggg tgagcgccgc ccgcaggccg tgcttgacga
     5041 cgacgtcgcg ctcccgcagg cccttggccc gtgcggtgcg gatgtagtcc tcgttcatcg
     5101 tctcgagcat ccctgcccgg gtgagccgcg cgtaggccgc cgcgtagagg aaggccaggg
     5161 tgatccaggg caggatgagc cggtaggccc agtcgagcgg gttcgccgcc tccatggggt
     5221 tcgacgcccc tggggcggtc cagccgaggc cgtagctgaa gatcgacagg gagagcaggc
     5281 cggtgaagaa gatcggcagg gacacgccgg ccagcgcgac ggtcatcatg gtccggtcca
     5341 ggcgcgagcc gcgcttgagc gccgagacga cgccggtggc gacccctccg acgagccaga
     5401 ggacggcggc ccccgaggcc agcgacatcg tcacggggag ccggttgagg atctcgggca
     5461 ggaccgggtt ctgggtgacg aacgagtacc cgagacaggg cgccgggcag tgctcggtct
     5521 cggggccgta gttgtagtcc tgcccggcga cgatgccctt ggcgaagcgg ccgtactgga
     5581 gccagatcgg gtcggtgaac ccgagcttga cagcggtctc gtggatctgc tcctgcccgg
     5641 cgctcttgcc gacgtagcgc gaggcgaggt cctcggcggt ggcgccgccc aggcggggga
     5701 cgaggaagaa gatcgcaaac gtgatcgcgc tgacgatgaa gagcaggacg acggctccga
     5761 tgagccgtct gacgaggaac gtcaccatgt gctgaggggc ggtgcccgtg gcggccacgg
     5821 cgagtgtgcc gcagccgcca cgagcggtgc cttcacctac cttcgcggtg tggtgtcggg
     5881 tcgggtcgcg gtatgacgcc cgctcccgga cgtcataccg gtcacttctt cgaggtgccg
     5941 gcggccgtgt agtcgtactc gccgaaggcg tcgttgatga agatgttcgt caccgtcgcc
     6001 gggcggaaca gcagcccctt ggcatagatc ccggggatga cgagggcgtt ctccatgacg
     6061 agctcgtcga tgtcgcccca cagcttctcg cgagcggggg cgtcggccgt gacgagggcc
     6121 ttgtccaaga gcgcgtcgac cgccgggatc ttgatcccga ggttggtgtt gcccgcgggg
     6181 cggatcgtgc ggctgtcgac gatctgggcc agggcgccga agccgtccgg ccagtcggcg
     6241 gcccagccga aggtgaggat gcccaggccg ttcgccttgg cgaagtccgg cttaccggca
     6301 tacagcttga agtagtcgcc ctgtgggaaa cccttgaggg tgagcttgat cccgatcttg
     6361 gccagcgact gctgcatggc ctcggccatg ttcttctcct tgggccgctc ggcccggtag
     6421 gacatgaccg tctcgaagcc gtccggcttg ccgcaggccg cgagcgcggc cttggccttg
     6481 gcctcgtcac cctgcggctt ggccttggcg tcatagaggt cgatctcccg atgtcccggg
     6541 atcttgggcg ggagcagcga ggttgcgatg tcgccaccgg tctcgccgcc gtaggcgcgc
     6601 tggtagccgg tcttgtcata gccgtagagg acggccttgc ggcactcgat gttgtcgagc
     6661 ggcggcacgt cggagttgat gttcgcgtac caggtccggg ccgacgcggc cgagtcggtc
     6721 ttggccttga ggtcggggtt gctgaggatc ttcgcctgcg actcggccgc gactccgctg
     6781 cccgcgaagt cgatgtcgag gtcgccggcc tggagccggt tgtcgacatc ggccgcgttg
     6841 agcccgagct cgacggtgag cgagtcgggc agcgccttgc ggccggagtc cttgtcggtg
     6901 gccgggtcgt agttctcgtt gcggacgagg acgtacctct tgtccgcctc gtaggaggag
     6961 aacttgtagg gcccggacga gacgaccttc tccttgtact tcgcgccggt gtccttggcg
     7021 gccgggaccg gggccgtctg cgggagcatg gcgaagtagt cgaagccgga gaaggacctc
     7081 ttgaggtgga agatgatggt cctgtcgtcc ggggtctcga tcgccgtaag gccggcgtcg
     7141 ctcttggacg ggtagaccgt gtacttggcg gggacgtcgg cgaggaagtt gttgaagtag
     7201 gtcggcccgt tggggaaggt gttcttgtcc agcgagcggg cgacggcgta cttgacgtcc
     7261 ttgctcgtga tcggcgtgcc gtcctcgaac ttcagcccgg acttgagctt gtaggtccac
     7321 gtcttgaagt ccttgctcgg caccccgagg gactcggcga ggtccggcac gagcacgttg
     7381 ccggccttgc ctggggccgc cttgaagacg acgagggtgc gggcatagtt gcgcacgaag
     7441 ttccacgagt agccgtagta catgtcgccc gggtcgaccg agtccaaagt gcccgccata
     7501 ccgaagcgca gcgtgccacc cttggtgtcc gaggggttga agaccgcgct caccgccgcg
     7561 ttcgccgctc cggccgcacc ggacgcgctc gtgcccgccg aggtgcccga cgagcccgag
     7621 caggcggaca ggccgagggc gaggacagcg cccgccacca ggacgggtcc tcgtttgctt
     7681 gtgttgcgca ttgcactcct ttgtggggga tggagccaga cccgcggcag ccggccggct
     7741 accgcgagcg ggggtcgagg gcgtcacgca ggccgtcgcc gaacaggttg aaggcgagga
     7801 cggtgacgaa gatggccagg ccgggaggga tcatgaactg gacgtcggtc tggtaccacc
     7861 cggccgcatc cgagagcatc ccgccccacg tcgcgcgggg gggctggacg ccgacgccga
     7921 ggaaggacag gcccgcctcg aagaggatgt tggccgggat gagcagggtg gcgtagatga
     7981 ggatcggggc gacgaggttg ggcagcagct cacggaagag gatgtagccg gtccggcccc
     8041 cgaggctgcg ggccgcgtcg acgaactccc gctcgcgcag cgacagggtc tgcccgcgga
     8101 tgatccggcc gatgtagggc cagttgaaga agccgatgat gaagatgagg atcgcgatcc
     8161 gcagggagtc gcccttgagc ccgaacgcct tgtccgggat cgccccgacg agcgcgaggg
     8221 cgaagacgag cagcgggaag gccaggaaga tgtccatgag gcggctgatg aggccgtcga
     8281 cccagcctcc ggtgaacccg gagacgacgc ccatgacggt gccgatgaag acggagagta
     8341 gcgtcgcgag gaaggcgatg agcagcgagg tctgggagcc gtagagcacc cggctgaaca
     8401 ggtcccggcc gttgatgggc tcgacgccga agaggaagtc cgtgctcacg ccgccgaacc
     8461 cgtgcagggg gagcccggtg gaggcgtcga cctggtcgta gtggaactcg tccggggggt
     8521 gcccgagcag ggtcacgacg agcggggcga ggaccgcgcc gacgatgagg acgacgacga
     8581 cgacaccccc ggcgatggcg acccggtcgc ggcgcagccg ccgccacgcg atctcgctca
     8641 gcgagcggcc ctggatcgcc gactcgccct cgaggaccgc gtcggtatcc agcgggtggg
     8701 tcggctcgat gtcgagtggg acggtcatcg tgcgtgccac ctccagcccc gtgttgcccg
     8761 atccgacctg cccaacacag acggacccgg ctcatcatgg tgtctggccg ggctggaggc
     8821 aaagcgctgt tgcgcaatcg tgactgcccg tgcgctcgtc ggcgaccctt cacagccagc
     8881 cgacatgggg cttgagcagg gtgtatccga cgaacgcgac gacatcgagc aaggtgtgcg
     8941 cgacgatgag cggccccacc cggccggctc ggcggtagac gagcccgaac acgaccccca
     9001 tgacgacgtt gccgaccgct ccgccgaagc cctggtag
//
LOCUS       DATAIX010000683         1833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_204893, whole genome shotgun sequence.
ACCESSION   DATAIX010000683 DATAIX010000000
VERSION     DATAIX010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204893"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1228
                     /gene="tdh"
                     /locus_tag="VHM65_10065"
     CDS             185..1228
                     /gene="tdh"
                     /locus_tag="VHM65_10065"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEX2706093.1"
                     /translation="MRALTKVSAGPGLALVDRPEPTPGPLDVKIRILRAGLCGTDLHL
                     EQWDDWAASTVAPPMVIGHEFYGEVVEIGEDVASVRIGDRCSGEGHIVCGTCRNCRAG
                     RRHVCINTVGVGVNRDGAFAEYVVIPASNVWVQPPDLDPDLGAVFDPLGNATHTALQW
                     PMVGEDVLITGAGPIGVMAAAIARHAGARHIVVTDVSDYRLALATGAGADLAVNVATT
                     SIGQAQRTLGMREGFDIGLEMSGAPSAVEEMLANLNHGARVAMLGLPKAPYPIDWGRV
                     ITHMITLRGIYGREMYDTWYTMSSMLQTSTPLREAIGSVITHRFPASDWQRAFDTARS
                     GQCGKVVLDFSVS"
     gene            1312..>1833
                     /locus_tag="VHM65_10070"
     CDS             1312..>1833
                     /locus_tag="VHM65_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2706094.1"
                     /translation="MYGSVRDELTATLEEIRAAGLYKHERELTSAQSAHVTTRHAAAL
                     NFCANNYLGLADHPEVVAAARDALAKWGFGMASVRFICGTQTQHIELESRLSAFLGTE
                     ATILFSSCFDANGGIFEVLFGSEDAIISDELNHASIIDGIRLSKAARFRYHNADMADL
                     ETQPQAAEGARRRV"
BASE COUNT          281 a          650 c          620 g          282 t
ORIGIN      
        1 gcgggaaccg gccgggcccc tcctgcggga aggtcgcgcc ctcggccatg acttctcctc
       61 gttcgaacgc cgttcgcagc gtcagtcgga tcgtgcatca ggtggccaga gcgtagtccc
      121 actctggcta ccccagaaga gggggcgccc ccgctcctga tacatccggc gctagagtgc
      181 tggggtgcgc gcactgacga aggtgtcagc gggtcccggg ctggccctgg tcgaccggcc
      241 cgagccgacc cctggaccgc tcgacgtgaa gatccggatt ctgcgggcgg gtctgtgcgg
      301 caccgacctg cacctggagc agtgggacga ctgggcggcc tccaccgtgg cgcccccgat
      361 ggtcatcggc cacgagttct acggtgaggt cgtcgagatc ggcgaggacg tcgcgagtgt
      421 ccgcatcggt gaccgatgct cgggcgaggg gcacatcgtg tgcgggacct gccgcaactg
      481 ccgggccggt cggcgccacg tctgcatcaa cacggtcggg gtgggcgtca accgggacgg
      541 cgcctttgcc gagtatgtcg tgatccccgc ctcgaacgtg tgggtccagc caccggacct
      601 cgacccggac ctcggcgccg tcttcgaccc gctcggcaac gcgacccaca cggcgctgca
      661 gtggcccatg gtcggtgagg acgtcctcat caccggcgcc ggcccgatcg gcgtcatggc
      721 cgccgcgatc gcgcgccacg ccggggcccg gcacatcgtg gtgaccgacg tcagcgacta
      781 ccgcctggcc cttgccacgg gggccggcgc cgacctcgcc gtcaacgtgg cgacgacctc
      841 gatcgggcag gcccagcgca ccctgggcat gcgggagggc ttcgacatcg gcctggagat
      901 gtcaggggcg ccctcagcgg tcgaggagat gctcgccaac ctcaaccacg gtgcccgggt
      961 cgcgatgctg ggtctgccga aggcgccgta cccgatcgac tggggtcggg tgatcaccca
     1021 catgattacg ctcagaggga tctacggccg tgagatgtac gacacgtggt acacgatgag
     1081 ctcgatgctg cagacgtcca ccccgctgcg ggaggcgatc gggtcggtca tcacccaccg
     1141 cttccccgcg agcgactggc agcgggcctt cgacacggcc cggtccggtc agtgcggcaa
     1201 ggtcgtcctg gacttcagcg tcagctaagg gctcgccgtg ctcccgtggc tgaccgacac
     1261 cccggcatac tctggtgtgc cggggttgac atccgcacaa aggagcgcag catgtacggg
     1321 tcggttcgcg acgagctcac cgccacgctg gaggagatcc gggcggccgg cctctacaaa
     1381 cacgagcgcg agctgacctc ggcgcagtcg gcgcacgtga cgacgcgcca cgccgccgcc
     1441 ctcaacttct gcgccaacaa ctacctgggt ctggccgacc accccgaggt cgttgccgca
     1501 gcgcgagatg cgctggcgaa gtggggcttc ggtatggcga gtgtccgctt catctgcggc
     1561 acgcagaccc agcacatcga gctcgagtcc cggctctccg ccttcctcgg caccgaggcg
     1621 acgatcctgt tctcctcgtg cttcgacgcc aacggcggga tcttcgaggt cctcttcggc
     1681 agcgaggacg cgatcatctc cgacgagctc aaccacgcct cgatcatcga cggcatccgc
     1741 ttgtccaagg cggcgcgctt ccgctaccac aacgccgaca tggccgacct cgagacccag
     1801 ccccaggctg ccgagggggc tcggcgccgg gtc
//
LOCUS       DATAIX010000684         3338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_295054, whole genome shotgun sequence.
ACCESSION   DATAIX010000684 DATAIX010000000
VERSION     DATAIX010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295054"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VHM65_10075"
     CDS             complement(<1..840)
                     /locus_tag="VHM65_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX2706095.1"
                     /translation="MSIAYPIEERRLPNGLRVVVSEDHTVPTVTVSLWVGVGSRHEVA
                     GRTGFAHLFEHLMFQGSRHVASGEHMSALMAQGSRLNATTWFDRTNYFETVPKGALEL
                     ALWLEADRHGYLLDALSQENLDNQRDVVKEEKRQRYDNVPYGTALIDVYATLFPPGHP
                     YHHATIGSMEDLDAASLDDVHAFYRRYYGPSNSVLTLVGDVSSDEAFAAVERYFGDLP
                     ASAEPPAGPGPQLEPLPEPARLERRQAVPASRLYLAFRLPVDATPEFYAAALAIDAIA
                     ALGT"
     gene            complement(850..2214)
                     /locus_tag="VHM65_10080"
     CDS             complement(850..2214)
                     /locus_tag="VHM65_10080"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2706096.1"
                     /translation="MAPQGSRHHVVVIGSGFGGLFCAKALRRADVDVTVIARTSHHLF
                     QPLLYQVATGILSEGEIAPATREVLNRQANATVLVGDVVGIDLDARTVTSRVLHRDTV
                     TSYDSLVVAAGAGQSYFGNDHFAAHAPGMKSVDDALELRGRILGCFELAEVAAGPDGS
                     GSGAEVERLLTFVVVGAGPTGVEMAGQISELARRTLRRDFRRVDPTRARVVLVDGAAQ
                     VLPTFGHRLGEKARARLESMGVEVVLGSLVVDLDATGIVLKGPDGTTRRIEAVCKVWA
                     AGVQASSLGRTLADQTGAALDRAGRISVRPDLSLPGRPEVFVIGDMIALDGLPGVAQV
                     AIQGARFAARQIANRLAGRPDEVRFVYHDKGSMATISRFSAVASIGSWRFGGFLAWVL
                     WLVIHLVYIIGFKHKVTTLLHWAVSFLGRGRSERTITDQQVYARRAIEALGTDSTPRF
                     PPTT"
     gene            2337..2921
                     /locus_tag="VHM65_10085"
     CDS             2337..2921
                     /locus_tag="VHM65_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706097.1"
                     /translation="MTSPGSGATRLLRSGVVAASTVALASAAHAQGGGTLPSGPGLAV
                     LTVLTACVVGVGTGRRLGVPAIGAMLGLGQVCLHLALGALGSGAATTAHAMVHGRAVA
                     HLPAAHLAHTTHSSGRMLLTHALATALTAILLAFGERTLWALWERVRPRPAVATTRLR
                     LGVARRALGPGEVCRPATPSFRWPPLRGPPPVTV"
     gene            3063..>3338
                     /locus_tag="VHM65_10090"
     CDS             3063..>3338
                     /locus_tag="VHM65_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706098.1"
                     /translation="MNSISPSRRALVAAAVVPTVLAALTACGSGTTSAGATSPVSGSS
                     STTSMADDDHEIGLTSAWVKAADSGMTAAFGMIVNESHEEVTLVKAAF"
BASE COUNT          465 a         1227 c         1171 g          475 t
ORIGIN      
        1 ggttcccagc gcggcgatcg cgtcgatggc cagcgccgcg gcgtagaact ccggtgtcgc
       61 gtcgaccgga aggcggaagg caaggtagag ccgcgaggcc gggacggctt ggcggcgttc
      121 gagtcgggcc ggctcgggca ggggctcgag ctgcgggccc gggcccgcag ggggctcggc
      181 gctggccggc aggtccccga agtagcgctc gaccgcggcg aaggcctcgt cggaggacac
      241 gtcaccgacg agggtcagca ccgagttgct gggaccgtag tagcgccggt agaaggcatg
      301 gacgtcgtcg aggctggccg cgtcgaggtc ctccatcgac ccgatcgtcg cgtggtggta
      361 ggggtgcccc ggggggaaca gcgtcgcata gacgtcgatg agcgccgtgc cgtagggaac
      421 gttgtcgtag cgctggcgct tctcctcctt gacgacgtcg cgctggttgt cgaggttctc
      481 ctggctgagc gcgtcgagga ggtagccgtg ccggtcggcc tccagccaga gcgccagctc
      541 gagggcaccc ttggggacgg tctcgaagta gttggtccgg tcgaaccagg tcgtggcgtt
      601 gagccggctc ccctgggcca tcagcgcgct catgtgctcg ccgctcgcga cgtggcggga
      661 cccctggaac atcaggtgct cgaagaggtg ggcgaagccg gtgcgaccgg ccacctcgtg
      721 gcgcgaaccg acgccgaccc acaggctcac ggtcacggtc ggcacggtgt ggtcctcgga
      781 cacgacgacc cgcaggccgt tcgggagacg acgctcctcg atggggtagg cgatgctcac
      841 gtgcagacct tatgtggtcg gcgggaagcg tggtgtgctg tcggtcccga gcgcctcgat
      901 cgcgcggcgg gcgtacacct gctggtcggt gatggtccgc tcggagcggc cacgcccgag
      961 gaagctgacc gcccagtgca gcagcgtcgt gaccttgtgc ttgaagccga tgatgtagac
     1021 gaggtggatc acgagccaca ggacccaggc gaggaagccg ccgaagcgcc acgacccgat
     1081 gctggccacc gcgctgaacc gcgagatcgt cgccatcgag cccttgtcgt ggtagacgaa
     1141 ccggacctcg tcgggtcgcc cggccagccg gttcgcgatc tgacgggccg caaaccgggc
     1201 accctgaatc gccacctggg ccacgcctgg cagcccgtcg agggcgatca tgtccccgat
     1261 gacgaacacc tcagggcgac cgggcaggct cagatctggc cggaccgaga tccggccggc
     1321 ccggtcgagc gccgcgccgg tctggtcggc cagcgtccgg cccaggctgc tcgcctgcac
     1381 cccggcagcc cagaccttgc acaccgcctc gatccggcgg gtggtgccgt cggggccctt
     1441 gaggacgatc cccgtcgcgt cgaggtcgac caccaaggag cccagcacga cctcgacccc
     1501 catcgactcc agccgggccc gcgccttctc ccccagccgg tgcccgaagg tcggcaggac
     1561 ctgggcggcc ccgtcgacga ggacgacccg ggcccgggtg gggtcgaccc ggcggaagtc
     1621 gcgccgcagg gtgcgccggg cgagctcgct gatctggccg gccatctcga cgcctgtggg
     1681 cccggctccg acgacgacga aggtcagcag ccgctcgacc tctgcgccgc tccccgagcc
     1741 gtccgggccg gccgcgacct cggccagctc gaagcagccg aggatccggc ctctcagctc
     1801 gagggcgtca tcgacgctct tcatccccgg cgcgtgtgcg gcgaaatggt cgttgccgaa
     1861 gtaggactgg cccgcccctg ccgccaccac cagcgagtcg tatgacgtga cggtgtcccg
     1921 gtgcagcacc cgcgacgtga ccgtcctcgc gtcgaggtcg atcccgacga cgtcgccgac
     1981 gagaacggtc gcgttggcct gacggttgag cacctcgcgg gtcgccgggg cgatctcgcc
     2041 ttcggagagg atgcccgtcg cgacctggta gagcagcggc tggaagaggt ggtggctcgt
     2101 gcgcgcgatg accgtcacgt ccacgtcggc ccggcgcagc gccttggcgc agaacaggcc
     2161 gccgaacccc gagccgatga cgacgacgtg gtgacgactt ccctgcggtg ccatggctcc
     2221 agtctgcctg acgggtaagg ctcacctgac cccgagggca cggcgctgct gaccccgagg
     2281 gcacggcgct gctgaccccg ctggctccgc cgccgtgcgt gggtaccctg tgaggcatga
     2341 ccagtcccgg gagcggcgcc acgaggctgc tgcgttccgg cgtggtcgcc gcgagcaccg
     2401 tggcgctcgc gagcgccgcc cacgcgcagg gcggcggcac cctgccctcg ggtcccggcc
     2461 tggccgtcct caccgtgctc accgcgtgcg tcgtcggggt cggcaccggg cggcgcctcg
     2521 gagtccccgc gatcggcgcg atgctcggtc tcggacaggt gtgcctgcac ctcgccctcg
     2581 gcgccctcgg ctccggcgct gcgaccacgg cgcacgcgat ggtgcacggg cgagccgtcg
     2641 cccacctccc ggccgcgcat ctggcccaca ccacgcacag ctcggggcga atgctcctca
     2701 cccatgcgct cgcgaccgca ctgaccgcca tactcctcgc gttcggcgag cgaacgctct
     2761 gggccctgtg ggagcgggtg cgtccgcgcc ccgccgtggc gacgactcgc ctgcgcctcg
     2821 gtgtcgcccg tcgcgccctc ggcccgggcg aggtatgccg tcccgccacg ccgtccttcc
     2881 ggtggccgcc actgcgcggt cctccgcctg tcaccgtctg agctgccacc actgcgctgc
     2941 caccactgcg ctgccaccac tgaccgcccg accgggcgtc atctcgtcgc gcccagcgca
     3001 caccactttg acatttgccc gcccatcgcc ggtgcggtcc cgaactgaaa ggaagaaccg
     3061 acatgaacag catctccccc tcgcggcgag ccctcgtggc cgccgcggtt gtccccaccg
     3121 tcctcgccgc cctcaccgca tgcgggagcg gcacgacctc ggccggtgcc acctcccccg
     3181 tctccggctc ctcgtcgacc acctcgatgg ccgacgacga ccatgagatc ggcctcacca
     3241 gcgcttgggt caaggccgcc gacagcggca tgaccgcagc gttcggcatg atcgtcaacg
     3301 agagccacga ggaggtgacc ctcgtcaagg ccgcgttc
//
LOCUS       DATAIX010000685         2117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_258346, whole genome shotgun sequence.
ACCESSION   DATAIX010000685 DATAIX010000000
VERSION     DATAIX010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258346"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VHM65_10095"
     CDS             <1..617
                     /locus_tag="VHM65_10095"
                     /inference="COORDINATES: protein motif:HMM:NF040399.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6390 family protein"
                     /protein_id="HEX2706099.1"
                     /translation="AWPYLELIAGASGIDDPLDARVVEAYWVGNGLLDNVTSSLLHRS
                     LEERFRGRAGRVWPRLADTAWAGAVPHHSFHVFGVYPWLGLLRSGQLAEPLQVLDRCR
                     IRWGQVLEVGSGFATVRSRPLRWDQSRHELTLGEARPERVQLAVDGLALASDLAPGQW
                     CALHWDWVCDRLDPGRLRALRDYTCRQLRVVSSPSYSPPAAVLS"
     gene            complement(612..1094)
                     /locus_tag="VHM65_10100"
     CDS             complement(612..1094)
                     /locus_tag="VHM65_10100"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX2706100.1"
                     /translation="MTSITLIAAVGRNRVIGDGTSMPWHLPVDLAHFRHVTMGHPLVM
                     GRRTFDSIGRALPGRRTIVITRQPGWHHGDVETAHSFADALALAGPADEVFVAGGGEV
                     YAEAMPFAHRLLVTEVDKSPQGSVVFPEIDPQHWHEVAREPHEGLSFVTYERRSRSAS
                     "
     gene            complement(1091..1900)
                     /locus_tag="VHM65_10105"
     CDS             complement(1091..1900)
                     /locus_tag="VHM65_10105"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX2706101.1"
                     /translation="MRQYLDLLGHVLEHGAAKSDRTGTGTRSVFGYQMRFDLSEGFPA
                     VTTKRLHLRSIIGELLWFLRGDTNVRWLQERGITIWDEWADAEGNLGPVYGYQWRSWP
                     TPDGRRVDQIARVIDSIRTNPDSRRHIVSAWNVADVDDMALPPCHTMFQFYVSPAAPG
                     RRARLSCQLYQRSADIFLGVPFNIASYALLTQMVAQVCDLDVGDFVHTIGDAHLYLNH
                     VDQAREQLGRAPYPLPLMRIAPGKGIDELDLPDFELVGYEAHPAIKAPIAV"
BASE COUNT          291 a          708 c          790 g          328 t
ORIGIN      
        1 gtgcgtggcc gtacctcgag ctcatcgccg gagcctcggg gatcgacgac ccgctcgacg
       61 cgcgggtcgt cgaggcctac tgggtcggca acgggctcct cgacaacgtc acgtcgtccc
      121 tgctgcatcg ctcgctcgag gagcgtttcc gcggccgggc cgggcgggtg tggccccgcc
      181 tcgcggacac cgcctgggct ggtgccgtgc cgcatcacag cttccacgtc ttcggggtct
      241 acccgtggct ggggctgctc cggtcggggc agctggccga gccgctgcag gtgctcgacc
      301 ggtgccgcat ccggtggggc caggtgctcg aggtgggctc cgggtttgcg acggtccgct
      361 cccgcccgct ccggtgggac cagtcccggc acgagctgac cctgggtgaa gcccggccgg
      421 agcgcgtcca gctcgccgtc gacgggctgg ccctggcctc ggatctggcg ccagggcaat
      481 ggtgcgcgct gcactgggac tgggtctgcg atcgcctcga cccgggtcgg ctccgagcgc
      541 tgcgggacta cacgtgccgt cagctgcggg tggtcagctc gccctcatac tccccgcccg
      601 cagccgtcct gtcatgaggc gctcctggag cggcgttcgt aggtgacgaa ggacagcccc
      661 tcgtgcggct cgcgggcgac ctcgtgccag tgctgcgggt cgatctccgg gaagaccact
      721 gacccctgcg gtgacttgtc gacctcggtg acgagcaggc ggtgcgcgaa cggcatcgcc
      781 tcggcgtaga cctcaccgcc cccggcgacg aacacctcgt cggccgggcc agccagcgca
      841 agggcgtcgg caaacgagtg cgccgtctcg acgtcgccgt ggtgccagcc gggctggcgg
      901 gtgatgacga tcgtgcgccg tccgggcagg gcccgcccga tcgagtcgaa ggtccgccgc
      961 cccatgacga gcgggtgccc catggtgacg tgtctgaagt gggcgagatc caccggcagg
     1021 tgccacggca tcgaggtgcc gtcaccgatg acccggttgc gcccgacggc ggcgataagg
     1081 gtgatggacg tcataccgcg atcggggcct tgatcgccgg gtgcgcctcg tagccgacca
     1141 gctcgaagtc gggcaggtcg agctcgtcga tccccttgcc cggcgcaatg cgcatcaacg
     1201 gcaaggggta tggcgcccgg ccgagctgct cgcgggcctg gtcgacgtgg ttgaggtaga
     1261 ggtgcgcgtc gccgatggtg tggacgaagt cgccgacgtc gaggtcgcag acctgggcca
     1321 ccatctgggt gagcagggcg tatgaggcga tgttgaacgg caccccgagg aagatgtcgg
     1381 ccgagcgctg gtagagctgg caggagagcc tggcccgccg gccgggagcc gccgggctga
     1441 cgtagaactg gaacatcgtg tgacaggggg gcagcgccat gtcgtcgacg tcggcgacgt
     1501 tccaggcgct gacgatgtgc cgacgtgagt cggggttggt gcggatcgag tcgatgaccc
     1561 tggcgatctg gtcgacccgc cggccgtcgg gggtcggcca cgaccgccac tggtagccgt
     1621 agacgggccc gaggttgccc tcggcgtcgg cccactcgtc ccagatcgtg atcccgcgct
     1681 cctgcagcca gcgcacgttg gtgtcgcccc gcaggaacca caggagctcg ccgatgatcg
     1741 agcgcaggtg cagccgcttg gtcgtcacgg cggggaagcc ttcggacagg tcgaagcgca
     1801 tctggtagcc gaacacgctg cgagtcccgg tcccggtgcg gtcgctcttg gcggccccgt
     1861 gctcgaggac gtgtccgagc aggtcgaggt actgacgcac acggcgagcc tatcccgtcc
     1921 caccggtcgc aggtcgggtt ggcctcgggc gatagcctga cggcatgagt atgacgccac
     1981 acctcggccg ggtgctgacg gccatggtca gcccgatgag accggacggg tcgatcgacc
     2041 tcgacggcgc ccagcgggtg gcccgccatc tcgtcgagtc cgggcacgac ggggtcgtcg
     2101 tcaacgggac gactggc
//
LOCUS       DATAIX010000686         1528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_235097, whole genome shotgun sequence.
ACCESSION   DATAIX010000686 DATAIX010000000
VERSION     DATAIX010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235097"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..990
                     /locus_tag="VHM65_10110"
     CDS             73..990
                     /locus_tag="VHM65_10110"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX2706102.1"
                     /translation="MSVPSGWYDDPENPENLRYWDGVLWTAHRQPKKSPTADQSRIGR
                     AYPVAPASHPTGVSGTAPMDQPGPGAPPQSPWTTPSAGQGTQPTSGQPTQPSYGQPSY
                     PPVAPMPGAYPPGAWAITGPTTPDGQPLAGWGTRLGAYLIDAIIASIVALPLTGYPLV
                     KFIQAYASVMSDVVDQIQRGVADPVLPDTTEFAASVLGWLVWFSLANLLVVVLYQTFF
                     LVRTGATPGKKLLGLAVRLRDKPGPLTVAVALRRQLVTVGSSLGSLIPVVSFVIALLP
                     LVDGLWPLWDEKRQALHDKIAATNVVKTR"
     gene            complement(977..>1528)
                     /locus_tag="VHM65_10115"
     CDS             complement(977..>1528)
                     /locus_tag="VHM65_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX2706103.1"
                     /translation="WEYVPLGPFLGKSFATSISAWVLPLAALDGARVPLPVQDPAPLP
                     YLTGTGPGLTGFDIHVEVAWNGTVVARPEYRDMYWSPMQMLAHLTVNGATVSPGDLFG
                     SGTISGPAKDSRGSFLELSWNGTEPVTLGDGSTRTFLEDGDIVTMRAWAPGPDASRVG
                     LGEVSGAIRPARQLSAGGAVSAS"
BASE COUNT          220 a          566 c          531 g          211 t
ORIGIN      
        1 ctcttcgagt cgatcgagcg cgagcaggag cgccgcggca acctgtaacc gcggccgtgg
       61 gacgatgggc gcatgagcgt tccgtcaggg tggtacgacg acccggagaa ccccgagaac
      121 ctgcgctact gggacggcgt cctgtggacg gctcaccgcc agcccaagaa gtcgccgacc
      181 gctgaccagt cgcggatcgg ccgggcctac ccggtggcgc ccgcgagcca cccgaccggc
      241 gtgagcggca ctgcacccat ggaccagccc gggccggggg cgccgcccca gtcgccgtgg
      301 accacgccat cggccggcca gggcacccag ccgacctccg ggcagcctac ccagccgtcc
      361 tacgggcagc cgtcataccc gccggtagcc ccgatgccgg gcgcctaccc gccgggcgcg
      421 tgggccatca ccgggccgac gactcccgac ggccagcccc tggccggctg ggggacccgg
      481 ctgggcgcct acctcatcga cgccatcatc gccagcatcg tggccctccc cctgacgggc
      541 tacccgctgg tcaagttcat ccaggcctac gccagcgtca tgtcggatgt ggtcgaccag
      601 atccagcgcg gggtcgcaga tcccgtcctg cccgacacga cggagttcgc cgcgtcggtc
      661 ctgggctggt tggtgtggtt ctcgctggcg aacctgctcg tcgtggtgct ctaccagacc
      721 ttcttcctcg tccgcaccgg agcgaccccc ggcaagaagc tcctgggcct cgcggttcgc
      781 ctccgtgaca agcccggtcc gctcaccgtg gccgtcgcgc tgcggcgcca gctcgtcacc
      841 gtcgggtcca gcctcggcag cctgatcccc gtggtgagct tcgtcatcgc cctcctgccg
      901 ctggtcgacg ggctctggcc gctctgggac gagaagcgtc aagcgctcca tgacaagatc
      961 gcggcgacca acgtcgtcaa gacgcgctga cggcgccgcc ggcgctcagc tgacgagcgg
     1021 ggcgtatggc gccgctcacc tcgccgaggc ccactcgact tgcgtccggg ccaggggccc
     1081 acgcccgcat cgtgacgatg tcgccgtcct cgaggaaggt ccgggtggac ccgtcgccga
     1141 gagtgaccgg ctcggtgccg ttccacgaca gctcgaggaa gctgccgcgg ctgtccttgg
     1201 ccggcccgct gatggtgccg gagccgaaga ggtcaccagg ggacaccgtg gccccgttga
     1261 cggtgaggtg ggcgagcatc tgcatcgggg accagtacat gtcgcggtac tccggccggg
     1321 ccaccacggt gccgttccac gcgacctcga cgtggatgtc gaacccggtc agccccggcc
     1381 cggtccccgt gagatagggc agcggtgcgg ggtcctggac cggcaggggc acgcgggcgc
     1441 cgtcgagggc ggcgagcggg agcacccacg ccgagatcga ggtcgcgaac gacttgccga
     1501 ggaagggccc gagcgggacg tactccca
//
LOCUS       DATAIX010000687         4074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_205138, whole genome shotgun sequence.
ACCESSION   DATAIX010000687 DATAIX010000000
VERSION     DATAIX010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205138"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1157
                     /gene="paaA"
                     /locus_tag="VHM65_10120"
     CDS             117..1157
                     /gene="paaA"
                     /locus_tag="VHM65_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEX2706104.1"
                     /translation="MYGNDVIDPNEPINAAETGEVEQAMAHEDRRDEVGSAFDALIAA
                     DQRIEPRDEMPEAYRRTLIRQIAQHAHSEIIGMQPEGNWVTRAPSLRRKAILIAKVQD
                     EAGHGLYLYGAAETLGVDRAELLDLLHAGKQKYSTIFNYPTLTWADNGAIGWLVDGAA
                     IMNQVALCRCSFGPYARAMIRICKEESFHQRQGFEILWTLMRGTPEQQAMAQESVNRN
                     WWPALMMFGPRDTGDAAIAGGHTAQSMAWGIKRFSNDELRQRFVDMTVAQAHKIGLTL
                     PDPHLRWNPERGHWDFGEIDWDEFWAVLRGDGPCNAERIGQRIEAHENGAWVREAAVA
                     YAAKQAARREAA"
     gene            1154..1450
                     /gene="paaB"
                     /locus_tag="VHM65_10125"
     CDS             1154..1450
                     /gene="paaB"
                     /locus_tag="VHM65_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HEX2706105.1"
                     /translation="MSTAESAAWPLWEVFVRAKRGLSHVHVGSLHAPDATMALRNARD
                     LYTRRQEGVSIWVVPAGQIAASSPDEKDAFFDPAADKTYRHPTFYHVPEDVEYL"
     gene            1447..2253
                     /gene="paaC"
                     /locus_tag="VHM65_10130"
     CDS             1447..2253
                     /gene="paaC"
                     /locus_tag="VHM65_10130"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEX2706106.1"
                     /translation="MTDNASVSTTRAPDAVGDEYAAAYLLGLADDALIYAQRLAEWLA
                     RAPELEEDMALANLSLDLIGQARALYTRAGELDGTGRSEDDFAYLRDELEFRNVHLVE
                     QKRGDFAHEMARLMWFSAYQGWLYGTSTAGSSDDTLRAIAGKAVKEVAYHFDHARQWV
                     LRLGDGTEKSHRRMQAALVSVAPYVEELFDDDAVSTTAAEAGLGRLPSSGRDWVHARV
                     GAVVEEATLTMPAPPGWRSRGGRDGMHSERMGFVLAELQHIARSHPGASW"
     gene            2256..2780
                     /gene="paaD"
                     /locus_tag="VHM65_10135"
     CDS             2256..2780
                     /gene="paaD"
                     /locus_tag="VHM65_10135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEX2706107.1"
                     /translation="MSSDVARIASADELAAAVAAIPDPEVPVLTIADLGILREVRIDG
                     LEAFVTITPTYSGCPAMTAIAQHIAHVCDQHGYAGYLTTRLDPAWTTDWMSEDGRAKL
                     ARYGIAPPHVLAGPPGGSPAGAVPVELGRRTVTCPQCGSGNTTEIAHFGSTACKALRR
                     CEECREPFDEFKAI"
     gene            2780..3889
                     /gene="paaE"
                     /locus_tag="VHM65_10140"
     CDS             2780..3889
                     /gene="paaE"
                     /locus_tag="VHM65_10140"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:TIGR02160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HEX2706108.1"
                     /translation="MTLTTTRPKRATFHALTVAATERLTEGSVALTFTVPAELRQDYL
                     FEPGQHLTLRATINGEEVRQSYSICQSRSEATASGTLRVACARIPGGRMSTWLTGSVE
                     PGAVIDVMTPVGSFVCPIRVDVARHHVAIAAGSGITPVMSLLTTALEEEPDSRVTLLF
                     GNRGTATIMFLEELEDLKNRFPSRFHLIHVLSREAPDAELFHGRLDPARLRLIFASLL
                     PVDSVDEWYLCGPYGLVIAAQELLADLGVDAHHIHHELFFVDDGTAPATPVVVDTSAP
                     PAAVVTVNLDGRTTVVPMPSRDESILAATLRARPDAPFSCTGGVCGTCRARLVEGEVR
                     MDRNYALEPDELAAGIVLACQSHPVTDRVVLDYDA"
BASE COUNT          630 a         1503 c         1380 g          561 t
ORIGIN      
        1 acgcatgccg ggcgcccggc ctgggctgac gtcaccgggc agtcgcaggg caaaatgcgt
       61 cgaacgccac gtcggtccgg ctagcaggcg cgcccgtcgc tcaccatact gaagtcatgt
      121 atggcaacga cgtcatcgac ccgaacgagc ccatcaacgc ggccgagacg ggtgaggtcg
      181 agcaggccat ggctcacgag gaccgccgcg acgaggtggg gagcgccttc gacgccctca
      241 tcgccgccga ccagcggatc gagcctcgtg acgagatgcc cgaggcgtac cgcaggaccc
      301 tgatccgcca gatcgcccag cacgcccact cggagatcat cgggatgcaa cccgagggca
      361 actgggtcac ccgggctccc tcgctgcggc gcaaggcgat cctcatcgcc aaggtgcagg
      421 acgaagcggg tcacggcctc tacctctacg gcgccgccga gaccctcggt gtcgaccggg
      481 ccgagctgct cgacctgctc catgcaggca agcagaagta ctcgacgatc ttcaactacc
      541 ccacgctgac ctgggccgac aacggggcga tcggctggct cgtcgacggc gcggcgatca
      601 tgaaccaggt tgccctgtgc cgctgctcct tcggccccta cgcccgggcc atgatccgga
      661 tctgcaagga ggagtccttc caccagcgcc agggcttcga gatcctctgg acgctcatgc
      721 gcgggacgcc cgagcagcag gcgatggcgc aggagtcggt caaccgcaac tggtggccgg
      781 ccctgatgat gttcggcccg cgggacaccg gcgacgcggc gatcgcgggc gggcacacgg
      841 cccagtcgat ggcgtggggc atcaagcggt tcagcaatga cgagctgcgt cagcgcttcg
      901 tcgacatgac ggtcgcccag gcccacaaga tcgggctgac cctgccggac ccccacctgc
      961 gctggaaccc cgagcgcggt cactgggact tcggcgagat cgactgggac gagttctggg
     1021 cggtcctgcg tggcgacggc ccgtgcaacg ccgagcggat cggccagcgg atcgaggcgc
     1081 acgagaacgg ggcctgggtg cgcgaggccg ctgtcgcgta cgccgcgaag caggccgcgc
     1141 gaagggaagc agcatgagca ccgccgagtc cgcggcctgg cccctctggg aggtcttcgt
     1201 ccgggccaag cgtggcctgt cgcacgtcca tgtcgggtcg ctgcacgccc cggacgccac
     1261 catggccctg cgcaacgcga gggacctcta cacgcggcgc caggaaggcg tctcgatctg
     1321 ggtcgtcccg gcggggcaga tcgccgcgag cagccccgac gagaaggacg cgttcttcga
     1381 cccggcggcc gacaagacct accgtcatcc gaccttctac cacgtgccgg aggacgtcga
     1441 gtacctatga ctgacaacgc atccgtgtcg accacccggg cccctgacgc cgtcggcgac
     1501 gagtacgccg cggcctacct gctcggcctg gccgacgacg cgctgatcta tgcccagcgg
     1561 ctcgcggagt ggctcgcccg cgcccccgag ctcgaggagg acatggcgct ggccaacctc
     1621 agcctcgacc tcatcggcca ggcgcgggcg ctgtacaccc gggccggcga gctcgacggg
     1681 accgggcgca gcgaggacga cttcgcctac ctgcgcgacg agctcgagtt ccgtaacgtc
     1741 cacctcgtcg agcagaagcg cggcgacttc gcccacgaga tggcgcggct gatgtggttc
     1801 tcggcctacc agggctggct ctacggcacg tcgacggccg ggtcgagcga cgacacgctg
     1861 cgggcgatcg ccggtaaggc ggtcaaggag gtggcctacc acttcgacca cgcccggcag
     1921 tgggtgctgc gcctcggcga cggcaccgag aagtcccacc ggcggatgca ggccgccctg
     1981 gtgagtgtcg cgccatacgt ggaggagctg ttcgacgacg acgcggtgag cacgacggct
     2041 gccgaggcag gcctgggccg gctcccgtcc tcgggccgcg actgggtgca cgctcgcgtc
     2101 ggcgcggtgg tcgaggaggc caccctgacg atgcccgcgc ccccggggtg gcgctcgcgc
     2161 ggtgggcgcg acggcatgca cagcgagcgg atgggtttcg tcctcgccga gctgcagcac
     2221 atcgcgcgct cccacccggg ggcgtcctgg tgatgatgag ctcagacgtg gccaggatcg
     2281 ccagcgccga tgagctcgcc gccgccgttg ccgccatccc cgaccccgag gtcccggtcc
     2341 tcaccatcgc cgacctcggc atcctgcggg aggtgcggat cgacgggctc gaggccttcg
     2401 tcaccatcac accgacctac tcgggctgcc cggcgatgac cgcgatcgcc cagcacatcg
     2461 ctcacgtctg cgaccagcac gggtatgccg gctacctcac cacccgcctc gaccctgcgt
     2521 ggacgaccga ctggatgagc gaggacggcc gggccaagct cgcgcgctac ggcatcgcgc
     2581 ctccccacgt cctggccggg cccccgggcg gctcgcccgc aggggcggtc cccgtcgaac
     2641 tcgggcgacg cacggtcacc tgcccgcagt gcgggtcggg gaacaccacg gagatcgcgc
     2701 acttcgggtc gacggcgtgc aaggcactgc gccggtgcga ggagtgccgg gagccattcg
     2761 acgagttcaa ggcgatctga tgaccctgac gacgacccgg cccaagcggg ccaccttcca
     2821 cgccctcacc gtcgcggcga ccgaacgcct caccgagggc tcggtcgcgc tgaccttcac
     2881 cgtgcccgca gagctgcgcc aggactacct cttcgagccg gggcagcacc tcacgctgcg
     2941 cgcgacgatc aacggcgagg aggtccgcca gtcctactcg atctgccagt cgcgctcgga
     3001 agccacggcg tcgggcaccc tgcgggtcgc ctgcgcgcgg atcccgggcg ggcggatgtc
     3061 gacctggctc accgggtcgg tcgagcccgg agccgtcatc gacgtcatga ccccggtcgg
     3121 gagctttgtc tgccccattc gggtcgacgt ggcgcgccac cacgtcgcga tcgccgccgg
     3181 ctcggggatc acgccggtga tgtccttgct caccacggcc ctcgaggagg agcccgacag
     3241 ccgcgtcacc ctgctcttcg gcaaccgggg caccgccacg atcatgttcc tcgaggagct
     3301 cgaagacctc aagaaccggt tcccgagccg cttccacctc atccacgtcc tctcccggga
     3361 ggcccccgac gccgagctct tccacgggcg cctcgacccg gcgcggctgc ggctcatctt
     3421 cgcctcgctg ctcccggtgg actcggtcga cgagtggtac ctctgcggcc cctacggcct
     3481 ggtgatcgct gcccaggagc tgctcgccga cctgggtgtc gacgcgcacc acatccatca
     3541 cgagctcttc ttcgtcgacg acggcaccgc accggctacg ccggtcgtcg tcgacacgag
     3601 cgcgccgccg gccgccgtgg tcaccgtgaa cctcgacggg cgcacgaccg tcgtcccgat
     3661 gccgagccgg gacgagtcga tcctcgcggc gacgctgcgt gcccgccccg acgcgccgtt
     3721 cagctgcacc ggcggggtgt gcggcacgtg ccgcgcccgc ctcgtcgaag gcgaggtccg
     3781 gatggaccgc aactacgcgc tcgaaccgga cgagctcgcg gcagggattg tcctggcctg
     3841 ccagtcccac ccggtcaccg accgcgtcgt gctcgactac gacgcctgac cggagtcacc
     3901 tgacccgccg cggccgcggc caggaccggt cacatccacc tcggtagtgc ggtgacggct
     3961 cactgaggtg gatccggccg gccaggaccg gtcacatcca cctcgatagt gcgggacggc
     4021 tcactgaggt cgatccggcc ggccggatcg acctcagtga gccgtcccgc acta
//
LOCUS       DATAIX010000688         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_295362, whole genome shotgun sequence.
ACCESSION   DATAIX010000688 DATAIX010000000
VERSION     DATAIX010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295362"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(574..1080)
                     /locus_tag="VHM65_10145"
     CDS             complement(574..1080)
                     /locus_tag="VHM65_10145"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX2706109.1"
                     /translation="MGALLDYLFWLRDRVLAAAASLSEGEFRSDKTVATRDLRATLVH
                     QLENEWAWRVRLSQGAFPGGDLDAEDYPTLDDLVNGWHREERELRTWFERVSDMQLAT
                     CPPGDDNVLPLWRYLVYVVTHGIQQFSEAAVLLTRLGHSPGEIGFLDFSSDPSLPARA
                     MPPAPWGS"
     gene            complement(1337..2071)
                     /locus_tag="VHM65_10150"
     CDS             complement(1337..2071)
                     /locus_tag="VHM65_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706110.1"
                     /translation="MRFHLKRREPRDTRPRLPPDEVAWIAEARRSFDDYSVGSLVPVV
                     FDRFARILHPAWSAAHGPVRWDVAAAWSGRTAHALAQWELLSVPITGAAQAPPFIAPP
                     DTGGLPRASLAALSTVLASHTSTPDRCYVGVWEGYGWPVAEWSGPEVLDLENRSFHVR
                     RGPISIAIEIGWHPTGDEVLSEPPTLMWPADRAWFVASDTDLDSTYLGGSGPLVDSLL
                     ACPELEVWPVTATDDISIGSDHINAR"
BASE COUNT          382 a          756 c          791 g          348 t
ORIGIN      
        1 ccgtcgccgt cgagaccaag ccgccggacc tgtgtgccgg ctaccggcag cttcggcacg
       61 ccgcccgggg agacgttgac ctggtggacg cggcctgtgc ggccggggac ggggctgctc
      121 atggccggga agcctacccg aagtgcgttc gcgggttgcc ggcgagcgta ccctgccagc
      181 gactcgggcg cgcggggcgc ggcacgtgga ctcgggctcg gcgcgggacc tcgggctcgc
      241 gcgagcgcgg aacatcacgc gggcagccag cacatcacgc gggcagccag cccgcgacgc
      301 taccgggcgg acgccatctc cacggcgtgc gccaggaccg cgtcgagcat ctttgcggtg
      361 aggcggcccg tgaacgtgtt ctgctggctc aggtggaacg tccaactacc atgttcacag
      421 tccgccgacg tgcatgacga gcgtcccgcg ggagcaggcc tggcggggtg gtgccgtatc
      481 agcaacccat cagcacttgc agccggtctc agatgcggtt gagaccggca gggagtgttg
      541 gtaagtcgtg atacatcagc gacttcgtcg gcgtcacgag ccccatgggg ccggtggcat
      601 cgcgcgcgcc gggaggctcg ggtccgagct gaaatcgaga aacccgatct cgcccggtga
      661 gtgtccgagg cgagtgagga gcaccgcagc ttccgagaac tgctggatgc cgtgggtgac
      721 cacatacacg aggtatcgcc agagtggaag gacgttgtcg tccccgggtg gacaggtggc
      781 aagttgcatg tcggacactc gctcgaacca cgtccgcagc tctcgctcct cgcggtgcca
      841 accattcacg aggtcatcga gggttggata gtcctcggca tccaggtcgc ccccaggaaa
      901 cgcaccctgg ctcagcctga cgcgccacgc ccattcgttc tcgagctgat gcacgagcgt
      961 tgcgcgcaag tcccgggtcg ccacggtctt gtccgaccgg aactcgccct ccgataacga
     1021 tgccgccgcg gcaagaaccc ggtcgcgaag ccagaacaga tagtcgagca gtgcgcccac
     1081 ttcctcgtgc cgcatcgtcg cagtatggat cgctgccgat cgtcgagcat cacgaccggg
     1141 cgaacacttc gtgccggtct cagatgcggt cgacatcgag gccggcttgg tcaatcgccc
     1201 gcctcagaac cgcgtcgagc atctttgcgg tgaggcggcc cgtgaacgtg ttctgctggc
     1261 tcggatcaca agtccaggag caacgttcac ttcccgcaga cgaatcgggc atcaactgcc
     1321 tgggcggtcg atcgagctat cgcgcgttga tgtggtcgct gccgatgctg atgtcgtcgg
     1381 tcgccgtgac cggccagacc tcgagctcgg ggcacgccag cagcgagtcg accagcgggc
     1441 cggacccgcc gagataggtc gaatcgaggt cggtgtcgga cgcgacgaac cacgcgcgat
     1501 ccgccggcca catgagcgtg ggcggttcgg acagcacttc gtccccggtc gggtgccacc
     1561 cgatctcgat agcgatcgag atcgggcccc ggcgaacgtg gaacgaccgg ttctcgagat
     1621 cgaggacctc gggtcccgac cattccgcga cgggccaccc gtagccctcc cagactccca
     1681 catagcaccg gtcgggcgtg ctcgtatggc tggcgagtac agtgctcagc gcggccaagc
     1741 tcgcgcgcgg caggccgcca gtgtccggcg gcgcgatgaa cgggggtgcc tgcgctgccc
     1801 cggtgatcgg gaccgagagg agctcccact gcgcgagcgc gtgcgcagtc cggcctgacc
     1861 acgcggcggc gacgtcccag cgcaccggcc cgtgagcagc cgaccaggca ggatgcagga
     1921 tgcgggcgaa gcggtcgaac acgaccggga cgagcgaacc caccgagtag tcgtcgaagg
     1981 accgccgggc ttcggcgatc catgcgacct cgtctggagg gagcctcgga cgggtgtccc
     2041 ggggctcgcg tcgcttgagg tggaagcgca tcgcccgatt gtggcgtggc tcacggggcc
     2101 gcgggcggcg tccatgtcac gactgaagca acaatccctg ccggtctcat ggtcgcttga
     2161 catcaacccc ggcgaggttg accgcccgcc tcagcacggc gtcgagcatc ggcgccgtca
     2221 accgacgtgc aaacacgttc tgctgcgatg ggtggtacga gcccaggacg tcgtagc
//
LOCUS       DATAIX010000689         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_205228, whole genome shotgun sequence.
ACCESSION   DATAIX010000689 DATAIX010000000
VERSION     DATAIX010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205228"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1011)
                     /locus_tag="VHM65_10155"
     CDS             complement(73..1011)
                     /locus_tag="VHM65_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706111.1"
                     /translation="MSQFTGGTIGNYSRLWLIITGGILLAVGAIMAITLGNIPFAGGT
                     MVLTGGIMALVGIGLIVVGIIVDRRAASTDALLTSGTPGTATITGLTQTGMYMNENPQ
                     IRMQLLVSLPGQTPYATQHTEIVPMMLLGRLNSGAPLQVRVDPMNLNRVAIDWGSSGL
                     GMSGLGGTMGGPVAMAAAPTMPGTTAGSAQDESLAQVQAAMAGAATGAAAPFSNPAQG
                     NLTVEQLRNYLRASGLEAQARVDSMEDTGKTVGDESVYTMEMTLMIPGKQEQKLPKSA
                     AMIPTAKKHKLFQGMTVPVKYEATNPNLLMVEWDKI"
     gene            complement(1084..1971)
                     /gene="sucD"
                     /locus_tag="VHM65_10160"
     CDS             complement(1084..1971)
                     /gene="sucD"
                     /locus_tag="VHM65_10160"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX2706112.1"
                     /translation="MSILVGRDTRLLVQGITGREGAFHAQQMQDYGTNIVAGMTPGKG
                     GQMALERPVPVFNTCHEAVSEQGANTSVIYVPAAGAPDAVLEAVDAGIKTIFCITEHI
                     PALDMLKVVHVVEQAGARLVGPNCPGATSVGTAKVGIIPGTIHRPGHVGVVSRSGTLT
                     YEVVEALTEAGIGQSTCVGIGGDPISGSNFIDILALFRDDPETTAVVLIGEIGGDGEE
                     RAAEWIAANMAGVPVASFIAGRTAPPGRRMGHAGAIISGGRGTAQSKVAALEAAGVRV
                     AEAPSQIPRLLLEMGVHTS"
BASE COUNT          289 a          751 c          715 g          387 t
ORIGIN      
        1 tcgagctgat cgaggccacc ggtggccccg cctgggtgtc gctcggctac gaatagcgga
       61 gtccggccaa cctcagatct tgtcccactc gaccatcagc aggttgggat ttgtcgcttc
      121 gtacttgacc ggcacggtca tgccctggaa gagcttgtgc ttcttggcgg tcggaatcat
      181 cgccgcggac ttgggcagct tttgctcctg cttgccgggg atcatcagcg tcatttccat
      241 cgtgtagacg ctttcatcgc cgacagtctt gccggtgtcc tccatcgagt cgacgcgagc
      301 ctgcgcctcg agcccgctcg ctcgcaggta gttgcgcagc tgctcgacag tcaggttgcc
      361 ctgggcgggg ttgctgaatg gtgccgccgc tcccgtggcc gctccggcca tggcggcctg
      421 gacctgcgcg agactctcgt cctgggctga acccgcggtc gtgccgggca tcgtcggcgc
      481 ggcggccatc gccaccggac cgcccatcgt gccgccgagg ccgctcatgc cgaggccgct
      541 cgagccccag tcgatggcga cgcggttgag gttcatcggg tcgacccgca cctgcagcgg
      601 ggcgcccgag ttgagccggc cgagcagcat cattggcacg atctccgtgt gctgcgtcgc
      661 gtacggcgtc tgaccgggca gcgacaccag cagctgcatc cggatctgcg ggttctcgtt
      721 catgtacatg ccggtctggg tcaggccggt gatcgtcgct gtgcccgggg tcccagatgt
      781 gagcagcgcg tccgtcgacg ccgcccggcg gtcgacgatg atgccgacca caatcaggcc
      841 tatcccgacc agggccatga tgccgcccgt caggaccatc gtcccgcccg cgaatgggat
      901 gttgccaagg gtgatggcca tgatggcccc gaccgcgagc aggatgccgc cggtgatgat
      961 cagccacagc cggctgtagt taccgatggt gccaccggtg aactgggaca cgtttcctct
     1021 cctatctccc gctggttaag tggggtataa cgagggtcct agagccggtt gtgacgcggg
     1081 ctcctagctt gtgtggacgc ccatctccaa cagcagccgt gggatctggc tcggcgcttc
     1141 tgcgacacgg actccggccg cctcaagggc agccaccttg ctctgcgccg tgccccgtcc
     1201 gccgctgatt atcgccccgg catgacccat gcgccgccct ggcggcgcgg tccggccggc
     1261 aatgaacgat gccaccggca cgcccgccat gttggctgcg atccactccg cggcgcgctc
     1321 ctcgccgtcg ccgccgatct cgccgatcag caccacggcg gtcgtctccg ggtcgtcgcg
     1381 gaagagcgct aggatgtcga tgaagttcga gccgctgatc gggtcgccac cgatgccgac
     1441 gcaggtcgac tggccgatgc ccgcctcggt cagcgcctcg accacctcgt aggtgagggt
     1501 gcccgagcgg ctgacgacgc cgacgtgccc gggccggtga atcgtgcccg ggatgatgcc
     1561 caccttggcc gtgcccacgg aggtcgcacc ggggcagttt ggcccaacca gccgcgcgcc
     1621 ggcctgctcg accacgtgca ccaccttgag catgtcgagc gcgggaatgt gctcggtgat
     1681 gcagaagatg gtcttgatgc ccgcgtcgac ggcttcgagc accgcgtccg gcgcgcccgc
     1741 ggcggggacg tagatgacgc tcgtgttggc gccctgttcg cttaccgcct cgtggcaggt
     1801 gttgaacacc ggtaccggcc gctcgagcgc catctggccg cccttgcccg gggtcatgcc
     1861 ggccacgatg ttggtgccgt agtcctgcat ctgctgcgca tggaacgcgc cctcacggcc
     1921 ggtgatgccc tgtacgagca ggcgtgtgtc gcggccgacg aggatgctca cgccgccacc
     1981 atcgcggccg ttgccgccgc gaccgcccgc tcgaccgcct cgtcgagtgt cgccgcggtc
     2041 tgcaggtcgg ggtcgcccgc ctcgtccagg atgcgcgccg cctcttcggc gttggtgccg
     2101 acgatgcgca ccaccatcgg cacgtggcgc tcctgcatgc gc
//
LOCUS       DATAIX010000690         2533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_205338, whole genome shotgun sequence.
ACCESSION   DATAIX010000690 DATAIX010000000
VERSION     DATAIX010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205338"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VHM65_10165"
     CDS             <1..770
                     /locus_tag="VHM65_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2706113.1"
                     /translation="DRVAERGKWDGHAAGAAAEVEDLQAAAQLGAAGCGDLADGGPDG
                     GGTGGMAVPLAALGHTITVVDPSPDALAALQRRATEAGLADRVTAVQGDTDTLAELLD
                     AESVDLVSCHGVLEIVDDPPTSMAALVRVLRSGGLLSLVVANRLGVVVAKALAGEFAQ
                     AKAALLSTDGRWGPADPLPRRFDVAGVESLLGDAGLVSHGCHGVRIVTDLVPSALLDS
                     DTHRGELLDLESALASHPDYAFLSQIGTSLHVLAHRP"
     gene            786..2078
                     /gene="dinB"
                     /locus_tag="VHM65_10170"
     CDS             786..2078
                     /gene="dinB"
                     /locus_tag="VHM65_10170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF003015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX2706114.1"
                     /translation="MSRRQYAAPQRTSTDPPDDSACTILHVDMDAFYASASLIDRPDL
                     VGRPVIVGGGGNRSVVLSATYEARKFGVTSAMPMARARRLCPQGIVLEPNYRRYAAMS
                     DAVMATFEAVTPLVEALSLDEAFLDVSGARRRLGSSATIGQSLRDTICDEQGITCSVG
                     VASTKFVAKLASGLAKPDGMIVVPPEEVLAFLHQLPVGALWGVGERTEESLHRLGLRT
                     VADIAHTPVETLRRALGEVSGSHLHALAWGRDERPVTPSRPDKSIGSDETFAYDIDDP
                     VVIHRELLKLSDRTAARVRAAGMMGRTISVKVRFADFTTITRAKTIRDYTDVSRDIYR
                     VARDLYDALGLQRARIRLVGVRVEGLVPAGEAPIQGLLDEPNHGWRDADRAVDQASAR
                     FGAGAVRPASLVTEGSVDRAGRDRQRPPGSHPERRDLS"
     gene            2187..>2533
                     /locus_tag="VHM65_10175"
     CDS             2187..>2533
                     /locus_tag="VHM65_10175"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HEX2706115.1"
                     /translation="MPLSEREQRMLQQMEQALYAEDPQFATRIQRQGAGGPSRRRLAI
                     GALGIIVGLGLVVVGAMNSSVWVGAAGFALMVAGAAYAFTPAKTTTALGTVEPDGSVH
                     PAGRGRANGKGSSG"
BASE COUNT          351 a          948 c          869 g          365 t
ORIGIN      
        1 gtgatcgtgt ggccgagcgc ggcaagtggg acggccatgc cgccggtgcc gccgccgagg
       61 tcgaggacct gcaggcagcg gcccagctcg gtgctgcggg atgcggcgac ctcgctgacg
      121 gcggcccaga cggcggcggc accggcggta tggccgtgcc ccttgccgcg ctcggccaca
      181 cgatcaccgt cgtcgacccc agcccggatg ccctggcagc cctgcagcga cgggccaccg
      241 aggccggcct ggccgaccgg gtcaccgccg tccagggcga caccgacacc ctcgccgagc
      301 tcctggacgc cgagtccgtc gatctcgtct cctgccacgg agtgctcgag atcgtcgacg
      361 acccgccgac ctcgatggcg gcgctcgtgc gcgtcctgcg ctccgggggg ctgctctccc
      421 tcgtcgtcgc gaaccgcctc ggtgtcgtcg tcgccaaagc cctcgccggc gagttcgccc
      481 aggccaaggc ggccctgctc agcaccgacg gacgctgggg cccggccgac ccgctgccgc
      541 gccgcttcga cgtcgccgga gtcgagtccc tgctcggcga cgcgggcctg gtcagccacg
      601 gctgccacgg cgtgcgcatc gtcaccgacc tcgtgccctc ggccctgctc gacagcgaca
      661 cccaccgcgg cgagctgctc gacctcgagt cggcactcgc aagccacccc gactacgcgt
      721 tcctcagcca gatcgggacc agcctgcacg tcctcgccca tcgtccgtga ccccggcagg
      781 gcccgatgag cagacgtcag tacgcagcac cgcagcgcac gagcaccgac ccaccggacg
      841 actccgcctg cacgatcctg cacgtcgaca tggacgcctt ctacgcgtcg gcgtcgctca
      901 tcgaccggcc cgacctcgtc ggcagaccgg tcatcgtcgg tggtggtggc aaccgctcgg
      961 tcgtactgtc ggcgacatac gaggccagga agttcggggt cacctcggcc atgccgatgg
     1021 cgcgcgcccg ccggctctgc cctcagggca tcgtcctgga acccaactac cgccggtatg
     1081 ccgcgatgtc ggacgcggtc atggccacct tcgaggcggt caccccgctt gtcgaggcgc
     1141 tctcgctcga cgaggccttc ctcgatgtct ccggggcccg gcgccggctc ggctcgtcgg
     1201 cgacgatcgg ccagtcgttg cgtgacacga tctgcgacga gcaggggatc acctgttcgg
     1261 tgggggtggc ctcaaccaag ttcgtcgcca agctcgcctc cggcctggcc aagcccgacg
     1321 gcatgatcgt cgtcccgccc gaggaggtgc tcgccttctt gcaccagctc ccggtcggtg
     1381 ccctctgggg cgttggtgag cgcaccgagg agtcgctgca ccggctcggg ctgcggaccg
     1441 tcgccgatat cgcccatacc ccggtcgaga ccctgcgccg ggcgctcggt gaggtcagcg
     1501 gctcgcatct gcacgccctg gcgtgggggc gtgacgagcg tccggtcacc ccaagtcggc
     1561 ccgacaagag catcggctcg gacgagacct tcgcctacga catcgacgac cccgtcgtca
     1621 tccaccgcga gctgctcaag ctgagcgacc gcacggcggc gcgggtccgg gccgcgggga
     1681 tgatggggcg cacgatctcg gtcaaggtcc gcttcgccga cttcacgacg atcacccgcg
     1741 ccaagacgat cagggactac accgacgtct cgcgcgacat ctaccgggtc gcgcgggacc
     1801 tttacgacgc gctcgggctc cagcgggccc ggatccgcct cgtcggagtg cgggtcgagg
     1861 ggctcgtccc ggccggtgag gccccgatcc agggcctgct cgacgagccc aaccacgggt
     1921 ggcgggacgc ggaccgggcc gtcgaccagg ccagcgcgcg gttcggagcc ggtgccgtgc
     1981 gcccggcgag cctggtcacc gaggggtcgg tcgaccgagc tggccgagac cgccagcggc
     2041 ccccgggttc ccaccccgag cgaagggacc tatcctaatg gcagaagcac cggtggccgc
     2101 tccctcgcaa ctgacctcag gcaacatttg ggtgcgctcg ggcgttacta ctcgtgagac
     2161 acgcgtccca gggaaggagg tcgacggtgc cgctctcaga gcgtgaacag cggatgctcc
     2221 agcagatgga gcaggccctc tatgccgagg acccccagtt cgcgacccgg atccagcgtc
     2281 agggtgcggg tgggccttcc cgtcgacgcc tcgccatcgg agcgctcggc atcatcgtcg
     2341 ggctcggcct ggtcgtcgtc ggcgcgatga actcgtcggt ctgggtcggc gcggcggggt
     2401 tcgccttgat ggttgccggg gccgcctacg ccttcacccc cgccaagacc acaacggccc
     2461 tcgggacggt cgagccggac gggtcggtcc atcccgccgg tcggggcagg gcgaacggca
     2521 agggctcctc ggg
//
LOCUS       DATAIX010000691         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_205368, whole genome shotgun sequence.
ACCESSION   DATAIX010000691 DATAIX010000000
VERSION     DATAIX010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205368"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..1093)
                     /locus_tag="VHM65_10180"
     CDS             complement(314..1093)
                     /locus_tag="VHM65_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX2706116.1"
                     /translation="MTATGGDSATASVQSVDRALTILGLLAAADGELGITDLANQLGV
                     HKSTASRLVATLEAHELVEQQQDRGKYRLGLGLMRLSAAAALTVDLVRVARSAVNTLS
                     AQTGETINVAILSGLSAFYVDQVGATSTLQSHNWVGQHIPLHATSNGKALLAFAPGEI
                     VDAVCRAPLPGYTALTITSPERLRAEIAQVARQGWALAVDELEPGLTALAATIRGADG
                     LTAGSISVSGPGFRLDGKLHTLAEQVHAAAENVSLRLGWRP"
     gene            1156..2019
                     /gene="purU"
                     /locus_tag="VHM65_10185"
     CDS             1156..2019
                     /gene="purU"
                     /locus_tag="VHM65_10185"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEX2706117.1"
                     /translation="MTTVVPGRDFILALACPDRPGIVHAVSGYLVEQGANIRESQQFE
                     DELSGRFFMRVMFRADGASVPGLRDAFGSVAQRLGMTWMLHDVGTPTRVLVMVSRFGH
                     CLNDLLYRWTVRSLNIDIVAIGSNHRDAEAVAGWHGIPFHHIPITAATKPAAEAELLS
                     LVDRYDVDLVVLARYMQVLSDDLCMALGGRAVNIHHSFLPSFKGARPYHQAHSRGVKL
                     IGATAHYVTPDLDEGPIIEQDVARVHHAYSPEDLVNAGRDVESQVLSRAIKWHSEHRV
                     FLNGPSTVVLR"
BASE COUNT          351 a          833 c          784 g          323 t
ORIGIN      
        1 ccgtcgcgca gcctcgatcg ccgccaccgc gtcctcggca ctggcctctc ggggaccgtg
       61 gcgacgagct caccgtcggc cgggcagcgg atctcgcgga gcccgccgag ggcagccaag
      121 cgccactcgc caccgatgaa cagggcgtcc acgcgggcta cctccccgtg tcggttgtgc
      181 gtccggcaga tcggtgtgtt gcacatcacg catcgcattg ctgttgaggc aacaggatgg
      241 gtgcgcggcg ggaccagcgt caagggttgg ccacggcgcg ggcgggtcgc aatctggtca
      301 aatggagccc tcgctaaggt cgccagccga ggcgcaggga cacattctcg gccgccgcat
      361 ggacctgctc ggcgagggta tggagcttgc cgtcgaggcg gaacccggga ccggacaccg
      421 agatcgagcc cgccgtcaac ccgtcggccc cgcggatcgt cgcggccagc gcggtgagcc
      481 ccggctcgag ctcgtccacg gcgagtgccc acccctggcg ggccacctgc gcgatctccg
      541 cgcgcaaccg ctcgggcgag gtgatcgtca gggcggtgta cccgggaagc ggtgcgcgac
      601 ataccgcgtc gacgatctcg cccggcgcga aggcgagcag ggccttgccg ttggacgtcg
      661 cgtgcagcgg gatgtgctgg ccgacccagt tgtgcgactg cagtgtcgag gtcgcgccga
      721 cctggtcgac gtagaacgcg gacagcccgg acaggatcgc gacgttgatc gtctcgccgg
      781 tctgggccga gagcgtgttg accgcggacc gcgccacccg gacgaggtcg acggtcaggg
      841 ccgcggcagc cgacaggcgc atcagcccga gcccgagccg gtacttgccg cgatcctgct
      901 gctgctcgac gagctcatgg gcctcgaggg tcgccacgag ccgagacgcg gtcgacttgt
      961 ggacgccgag ctggttggct aggtcggtga tgcccagctc gccatcggcc gccgccagca
     1021 gcccgaggat cgtcagggct cggtccaccg actggacgga ggcggtcgcg ctgtcccctc
     1081 ccgtcgcggt catgggccag actctagacg acagttgcgt atgacgcacc aacgcggcag
     1141 tgggaggtcc gggccatgac gaccgtcgtg cccggccggg acttcatcct ggcgctggcc
     1201 tgccccgacc ggccggggat cgtgcacgcg gtgagcggct acctcgtcga gcagggcgcc
     1261 aacatccgcg agtcccagca gttcgaggac gagctgtccg gccggttctt catgcgggtg
     1321 atgttccggg ccgacggcgc gtcggtgcca gggctgcggg acgccttcgg gtcggtcgcc
     1381 cagcggctcg ggatgacgtg gatgctgcac gacgtcggga ccccgacccg ggtcctggtc
     1441 atggtgtcgc gcttcgggca ctgcctcaac gacctgctct accgatggac cgtgcggtcg
     1501 ctcaacatcg acatcgtcgc gatcgggtcg aaccaccgcg acgccgaggc cgtggcgggc
     1561 tggcatggga tcccgttcca ccacattccc atcactgcgg cgaccaaacc cgcggcggag
     1621 gccgagctgc tgtcgctcgt cgaccggtat gacgtcgacc tcgtcgtgct cgctcgctat
     1681 atgcaggtgc tgtcagacga cctttgtatg gcgctcggcg gccgggcggt caacatccac
     1741 cactcgttcc tgccgagctt caagggggcc aggccctatc accaggccca ctcccggggg
     1801 gtgaagctca tcggcgcgac cgcccactac gtgacgcccg acctcgacga gggaccgatc
     1861 atcgagcagg acgtcgcccg ggtccaccat gcgtactccc ccgaggacct cgtcaacgcc
     1921 ggtcgcgacg tcgagtcgca ggtcctctcc cgggcgatca agtggcacag cgaacaccgg
     1981 gtcttcctca acggcccgag caccgtcgtc ctgcgctgag gccgggccgc accggccaca
     2041 accacctcac tatcgcggcc cacccgaacg aggccgatcc ggccgacccc caccggccag
     2101 aaccacctca ctaccggcgc ccgccagacc gaggccgaac cggccggccc gcaccggcca
     2161 gaaccacctc actacccccg gcccaccaga gcgaggccga accggccggc cctcgagctc
     2221 ccggccgcca accatgcgac gagggactgc cgcccagctg ggcggcagtc cctcgtcgca
     2281 tgcaggaggc t
//
LOCUS       DATAIX010000692         1747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_295842, whole genome shotgun sequence.
ACCESSION   DATAIX010000692 DATAIX010000000
VERSION     DATAIX010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295842"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..694
                     /locus_tag="VHM65_10190"
     CDS             95..694
                     /locus_tag="VHM65_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706118.1"
                     /translation="MPGVRQDLRRALLAIAFDQAGYFTAAQALVVGYSYQAQKYHIDH
                     GNWQRVDRGIFRIPDWPAKAEDTYIRWALWSGGRGVISHESALAVHDLSDVNPHRIHL
                     TVDPAFSRRDDPVVLHRARLADGDIEAHGAWSVTTVERTLLDVAQTFLSQEHVDAAVA
                     EAISRGLTTRRRLRLAGSGAPDRAALAIERALASAESIS"
     gene            849..1376
                     /locus_tag="VHM65_10195"
     CDS             849..1376
                     /locus_tag="VHM65_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HEX2706119.1"
                     /translation="MAAQRFADELNEQISREFAAHQQYLAAAVYYDRLTMPKMAGLFY
                     AQALEERQHAMMMVQYLLDTDARVMIPGIEAPVIEFADIAAPVALALAQEKRVTQQIY
                     ALTQTAREEMDFGSEQFMQWFIKEQVEEVASMTSLLAVVDRSHDDIRAIEEYVAAELG
                     AESPDPTAPAGAGYA"
     gene            complement(1367..>1747)
                     /locus_tag="VHM65_10200"
     CDS             complement(1367..>1747)
                     /locus_tag="VHM65_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706120.1"
                     /translation="AVFNHYVRSELGYANDLHYEQISNRVSPWSYKEFEGRAVDVTPR
                     LARAMRQNPHLRVHVSYGYHDGATPYYAAQDVIAHLKIPPELRANLEHAYYEAGHMMY
                     VHEPSRLQQSKDLAGFVRRASRQA"
BASE COUNT          281 a          578 c          600 g          288 t
ORIGIN      
        1 ggtggcttta ctcagcctga cttgtagttt gccgtgttct ggctgagcac aaacaataaa
       61 ttcggttgag tatctgccct gataggctcg ccgtgtgcct ggagtccgcc aagacctgcg
      121 tcgagccctc ctggcgattg ccttcgacca ggcaggctac ttcacggcgg cgcaggcgct
      181 ggtggtcggg tacagctacc aggcccagaa gtaccacatc gaccacggca actggcagcg
      241 ggtcgatcga ggcatcttcc ggattccgga ctggccggcc aaggccgagg acacctacat
      301 caggtgggcg ctctggagtg gtggacgtgg agtcatctca catgagtcgg ccctggccgt
      361 ccacgacctc tcggacgtca acccccatcg catccacctc accgtcgacc ccgccttcag
      421 caggcgtgac gaccccgtcg tcctgcaccg ggcccggctg gctgacgggg acatcgaggc
      481 gcacggagcg tggtccgtca ccacggtgga acgcacgctg ctcgatgttg cgcagacctt
      541 cctgtcgcag gagcacgtcg acgctgcggt ggccgaggcg atcagccggg ggctgacgac
      601 gcgccggcga ctgcgcctcg cggggagcgg cgcccctgac cgtgcggcgc tcgcgatcga
      661 acgtgctctg gcctcagcgg agtccatctc gtgactcagg ggcgccggga cctcgccggt
      721 ccagggcggg tccacaggtt cgtcgcgagt gacgtcatac gccgtggctc ggtaacgtcc
      781 ttcgcccccg ggccaaccgg gaccggcact gtctagactt ggacgacatc acccgaggga
      841 aggtcaccat ggcagcgcag cgtttcgccg acgagctcaa cgagcagatc tctcgcgagt
      901 tcgcagccca ccagcagtac ctcgccgccg cggtctacta cgaccgtctc acgatgccga
      961 agatggcggg cctgttctac gcccaggcgc tcgaggagcg ccagcacgcg atgatgatgg
     1021 tgcagtacct gctcgacacg gacgcccggg tgatgatccc cgggatcgag gcgccggtca
     1081 tcgagttcgc cgacatcgcc gctcccgtcg cgctcgcgct ggcgcaggag aagcgggtga
     1141 cccagcagat ctacgcgctg acgcagacgg cccgtgagga gatggacttc gggtccgaac
     1201 agttcatgca gtggttcatc aaggagcagg tcgaggaggt cgcgtcgatg acctcgctgc
     1261 tggccgtcgt cgaccggtcc cacgacgaca tccgggccat cgaggagtac gtcgccgccg
     1321 agctgggggc ggagagcccc gacccgacgg caccggcggg ggccggctac gcctgacggc
     1381 tcgcgcgccg gacgaagccg gcgaggtcct tgctctgctg gagccggctc ggctcgtgga
     1441 cgtacatcat gtgccccgcc tcgtagtagg cgtgctcgag gttggcgcgg agctcgggcg
     1501 ggatcttgag gtgggcgatg acgtcctggg cggcgtagta cggcgtcgca ccgtcgtggt
     1561 agccgtacga gacgtggacc cgcaggtgcg ggttctgccg catggcccgg gcgagccgcg
     1621 gggtcacgtc gacggcccgg ccctcgaact ccttgtacga ccacggcgag acccgattcg
     1681 agatctgctc gtagtgaagg tcgttcgcgt acccgagctc gctgcgcacg tagtggttga
     1741 acaccgc
//
LOCUS       DATAIX010000693         1740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_258685, whole genome shotgun sequence.
ACCESSION   DATAIX010000693 DATAIX010000000
VERSION     DATAIX010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258685"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VHM65_10205"
     CDS             complement(<1..692)
                     /locus_tag="VHM65_10205"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2706121.1"
                     /translation="MQITSSTVALVTGGASGLGGATTRRLVADGAKVVIVDLASSPGA
                     DVAAELGPAVRFVPADVRDGAAVQSAVDAALELGELRLAVNCAGVATPGRVLGRSGPL
                     DLDVFRTVVEINLVGTFNVLRLAAAAMTANAPVDGDRGVIVMTASIAAYDGQIGQAAY
                     AASKGGIVGLTLSAARDLADKLIRVMTVAPGTFETPMLAGLPSEVKAKLGAAVPHPAR
                     LGRPAEYAALVR"
     gene            complement(702..>1740)
                     /locus_tag="VHM65_10210"
     CDS             complement(702..>1740)
                     /locus_tag="VHM65_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2706122.1"
                     /translation="DVFALLGELGLLSLAYPEEYGGGGQPYEVYLQVVEEIATVWMSI
                     AVGVSVHGLTCFPVATFGTDAQKQALLPDMLSGSTLGAYCLSEPEAGSDVAAISTRAV
                     AELDAADEPTAYRLTGTKAWISHAGHADFYTTFVRTSHDGPRGLSCFVIPGGTEGLTF
                     SAPERKMGLHCDTVSQVHLDHARVAADRLIGGPGRGMTIALSALSAGRLGIAAAATGL
                     AQAALDRAVAYAKERRQFGQRIGDFQGLGFMLADMEASVLSARANYLQAARIKDSGRD
                     YAMHAAAAKLVCTDTAMKVTTDAIQILGGYGYTEDFAVERYFREAKVTQIFEGTNQIQ
                     RLVISRQLMKD"
BASE COUNT          251 a          606 c          643 g          240 t
ORIGIN      
        1 tggcgcacca gggcggcata ctcggccggc ctgccgagcc gggcggggtg cggcaccgca
       61 gcgccgagct tggccttgac ctcgctggga agcccggcga gcatcggcgt ctcgaaggtc
      121 cctggggcga cggtcatgac gcgaatgagc ttgtcggcga ggtcgcgcgc ggccgagagg
      181 gtgagcccga cgatcccgcc cttggacgcg gcatacgccg cctggccgat ctgaccgtcg
      241 tacgcggcga tcgaggcggt catgacgatg actccgcggt cgccgtcgac gggcgcgttg
      301 gcggtcatcg cggcggcggc gaggcgcagg acgttgaacg tcccgacgag gttgatctcg
      361 acgaccgtgc ggaagacgtc gaggtcgagc ggaccggagc gcccgagtac tcggccgggc
      421 gtcgcgaccc cggcgcagtt gacggccagc cgcagctcgc ccagctccag cgccgcgtcc
      481 actgccgact gcacggcagc accgtcgcgg acgtcggccg gcacgaaccg caccgccggc
      541 ccgagctcgg cggcgacgtc ggcgccgggc gaggaggcca ggtcgacgat gacgaccttg
      601 gctccgtcgg ccacgagccg ccgggtcgtg gcgccgccga gcccggaggc gccgcccgtg
      661 acgagggcaa cggtggacga cgtgatctgc acgggaggct cctagtcctt cataagctgg
      721 cggctgatga cgagccgctg gatctggttg gtgccctcga agatctgggt caccttggcc
      781 tcgcggaagt accgctcgac ggcgaagtcc tcggtgtacc catacccccc gaggatctgg
      841 atggcgtccg tggtcacctt catcgcggtg tcggtgcaga cgagcttggc cgccgccgcg
      901 tgcatggcgt agtcgcgccc ggagtccttg atccgggccg cctgcaggta gttggcccgc
      961 gccgagagga ccgaggcctc catgtcggcc agcatgaagc cgaggccctg gaagtccccg
     1021 atccgctggc cgaactggcg ccgctccttg gcatacgcga ccgcccggtc gagggccgcc
     1081 tgagccagac ccgttgccgc agcggcgatc ccgagccggc cggcggacag cgcggacagg
     1141 gcgatcgtca tcccgcgccc cggtcccccg atgagccggt cggccgccac ccgcgcgtgg
     1201 tcgaggtgga cctggctcac cgtgtcgcag tgcagtccca tcttgcgttc gggagcggag
     1261 aaggtgagcc cctcggtgcc gccggggatg acgaagcacg acagtccccg cggcccgtca
     1321 tgggacgtcc gcacgaaggt cgtgtagaag tccgcgtgcc cggcgtgcga gatccacgcc
     1381 ttcgtccccg tgagccggta tgccgtcggc tcgtcggctg cgtcgagctc ggctacggcg
     1441 cgggtcgaga tggcggcgac gtcggacccg gcctcgggct cggacaggca gtaggcgccg
     1501 agggtcgacc cgctgagcat gtccggcagc agggcctgct tctgggcatc cgtgccgaac
     1561 gtcgcgaccg ggaagcaggt caggccgtgg acgctgacgc cgacggcgat gctcatccag
     1621 acggtggcga tctcctcgac gacctgcagg tagacctcgt acggctgccc cccgccgcca
     1681 tactcctcgg ggtaggccag ggagagcagc ccgagctcac cgagcagcgc gaagacgtcc
//
LOCUS       DATAIX010000694         2589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_235817, whole genome shotgun sequence.
ACCESSION   DATAIX010000694 DATAIX010000000
VERSION     DATAIX010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235817"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..273)
                     /locus_tag="VHM65_10215"
                     /pseudo
     CDS             complement(52..273)
                     /locus_tag="VHM65_10215"
                     /inference="COORDINATES: protein motif:HMM:NF017847.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
     gene            complement(286..1137)
                     /gene="ychF"
                     /locus_tag="VHM65_10220"
     CDS             complement(286..1137)
                     /gene="ychF"
                     /locus_tag="VHM65_10220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX2706123.1"
                     /translation="MALTIGIVGLPNVGKSTLFNALTKNNVLAANYPFATIEPNVGVV
                     PLPDERLGHLAAIFRSDKILPATVSFVDIAGIVRGASEGEGLGNKFLATIREADAICQ
                     VVRVFSDDDVVHVEGAVSPSGDIETINTELILADLQTLEKALPRLEKESRVNKARRAA
                     LDNAVAAQKVLEAGETLYGAGAAAGVDLTEARALQLLTTKPFIYVFNLDEDQLTDPAL
                     HASLSGLVAPADSVFLNAKLEMDLAELDDEEARELLESVGVSEPGLDQLAHKGFHTLG
                     LQTYLTA"
     gene            complement(1187..>2589)
                     /locus_tag="VHM65_10225"
     CDS             complement(1187..>2589)
                     /locus_tag="VHM65_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX2706124.1"
                     /translation="LGDIDLRFDSPLVAPGVDASVSVKESITDLFATRTVSVDAIKPT
                     PAEVDAALGAAIRGVGLRFLTGALIVAFVLSVAVQEGRRRRPSRRHLVAVGSALALTC
                     GGMAAAVAGTYRQQRFASSTSTGVLQAVQRNAGLLGSVETRAEQAVPYVRNLIALSAA
                     LQDKFVPTDLSAPESARLLLVSDLHGANSYALMRSVIAEERIDAVVDSGDLINFGSAT
                     EAESAGIFAGIASLGVPYVFVGGNHDLSSPADGALLARLARVPNVVLLQPGKATYTVY
                     SLNGLRVAGFNDPRYFGDDAKDTAAKQQPAVEAFNLAMAGQPVLDLAVSHEPAGAAGI
                     ERAVARVHGHLHRATLDGSLIGVGSFTGGGLALHFVDDGDAELTEQPYAFDVAAFGST
                     CRLTSLTRYSYRNLISGRPAYDNVTIVNGATIADRAADAGQADGAPATEPGRACSRSL
                     GVSRTTVPGAAGGGGG"
BASE COUNT          404 a          884 c          928 g          373 t
ORIGIN      
        1 agggagcttc gattgatgcg tcggattggt acctgcgcgc ggagctggcc ctcagacgtt
       61 gaagcggaac tccaccacgt cgccgtcggc catgacgtag tccttgccct cgatgcggac
      121 cttgccggcc gccttcgccg ccgccatgga gccggcctcg acgaggtcgg cgaaggacac
      181 gacctcggcc ttgatgaacc cgcgctggaa gtcggtgtgg atcacgcccg ccgcctgggg
      241 cgccgtccag ccctggcgga tcgtccacgc ccgcgactcc ttgggtcagg cggtgagata
      301 ggtctgcagc cccagggtgt gaaaaccctt gtgtgccagc tgatccaggc caggctccga
      361 gacgccgacg gactcgagca gctcgcgggc ctcctcgtcg tcgagctcgg cgaggtccat
      421 ctcgagcttg gcgttgagga agaccgagtc ggcgggcgcc acgagcccgg acagcgacgc
      481 gtgcagggcc gggtcggtca gctggtcctc gtcgaggttg aacacgtaga tgaacggctt
      541 ggtcgtgagc agctgcaggg cccgcgcctc ggtgaggtcg actccggccg ccgcgcccgc
      601 gccatacagg gtctcccccg cctcgaggac cttttgcgcc gccacggcgt tgtcgagcgc
      661 agcgcgcctg gccttgttga cccgcgactc cttctccagc cgcggcagcg ccttctcgag
      721 ggtctgcagg tcggcgagga tcagctcggt gttgatcgtc tcgatgtccc ccgacgggga
      781 gaccgcgcct tcgacgtgga cgacgtcgtc gtcactgaag acccgcacga cctgacagat
      841 cgcgtcggcc tcgcggatgg tggcgaggaa cttgttgccg aggccctccc cctccgaggc
      901 gccccggacg atccccgcga tgtcgacgaa cgacacggtc gcgggcagga tcttgtccga
      961 cctgaagatc gccgcgaggt gcccgagccg ctcgtccggc agtggcacga ccccgacgtt
     1021 cggctcgatc gtcgcgaacg ggtagttcgc cgccaggacg ttgttcttcg tcagtgcgtt
     1081 gaacagggtg gacttgccga cgttgggcag gccgacgatg ccgatagtga gagccacaag
     1141 acggacagtc tactgacgca cgcccgggta tgacgcgcgc gtcacgtcag ccgccgccac
     1201 cgccggccgc gccgggcacg gtggtccggg acacgccgag ggagcgggag cacgcccggc
     1261 cgggctcggt cgccggggcc ccgtccgcct ggccagcgtc ggcggcgcgg tcggcgatcg
     1321 tcgcgccgtt gacgatcgtc acgttgtcgt aggccggccg gcccgagatg aggttgcgat
     1381 agctgtagcg cgtcagtgac gtgagccggc aggtcgagcc gaacgccgcg acgtcgaacg
     1441 cgtagggctg ctcggtcagc tcggcgtcgc cgtcgtcgac gaagtgcagg gcaagccctc
     1501 cgccggtgaa ggaccccaca ccgatgagcg acccgtcgag cgtcgcccgg tgcaggtggc
     1561 cgtggacccg ggcgacggcc cgctcgatcc ccgccgcacc ggccggctcg tgcgagaccg
     1621 cgagatcgag cacgggctgg cccgccatcg ccaagttgaa cgcctccacc gcgggctgct
     1681 gcttggccgc ggtgtccttg gcatcgtcac cgaagtagcg cgggtcgttg aacccggcca
     1741 cccggagccc attgagggag tagaccgtgt aggtcgcctt gccgggctgc agcagcacga
     1801 cgttgggcac ccgggccagc cgggcgagca gggcaccgtc ggccggcgag gataagtcgt
     1861 ggttgcctcc gacgaacaca tagggcaccc cgagcgaggc gatccctgcg aagatccccg
     1921 cggactcggc ctcggtcgcg ctgccgaagt tgatgaggtc accggagtcg acgacagcgt
     1981 cgatccgctc ctcggcgatg accgacctca tcagcgcgta ggagttggcc ccgtgcaaat
     2041 ccgacacgag caggagccgg gccgactcgg gagcggacag gtcggtcggg acgaacttgt
     2101 cctgcagggc agcagacaag gcgatgaggt tgcggacgta gggaacggcc tgctcggccc
     2161 gggtctcgac gctgccgaga aggccggcgt tgcgctggac ggcctggaga accccagtcg
     2221 aggtcgagct ggcgaaccgc tgctgccggt acgtcccggc cacggcggcc gccatacccc
     2281 cgcaggtaag ggcgagcgca cttcccaccg cgaccaggtg gcgccgggac ggccggcgac
     2341 gccggccctc ctggacggca acggacagga cgaacgcgac gatcagcgct ccggtgagga
     2401 agcgtagccc caccccgcgg atcgcagccc cgagagcggc gtcgacctcg gccggggtgg
     2461 gcttgatcgc gtcgaccgag accgtacgag tcgcgaagag gtcggtgatg ctctccttga
     2521 ccgacaccga cgcgtcgacg cccggggcga ccaggggcga gtcaaaccgc aggtcgatgt
     2581 ctcccagga
//
LOCUS       DATAIX010000695         4019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_205885, whole genome shotgun sequence.
ACCESSION   DATAIX010000695 DATAIX010000000
VERSION     DATAIX010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205885"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..740)
                     /gene="lipB"
                     /locus_tag="VHM65_10230"
     CDS             complement(78..740)
                     /gene="lipB"
                     /locus_tag="VHM65_10230"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX2706125.1"
                     /translation="MQIRHAGFAPDYADYEQTWALQREVHAGVVEGSIPDTVLLLEHA
                     AVYTAGKRTEPHERPTDGTPVVDVDRGGKITWHGPGQLVGYPIVRLPVPINVVAHVRR
                     LEEVMIRVCSDLGLAAARVDGRSGVWVPADHRGPDRKLGAIGVRVSRFVTMHGFALNA
                     CCDLAWADPIIPCGIPDAGVSSISRELGRDVTVAEILPLAERHLLDVLGPVSQAGAAR
                     AS"
     gene            897..3023
                     /locus_tag="VHM65_10235"
     CDS             897..3023
                     /locus_tag="VHM65_10235"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX2706126.1"
                     /translation="MPVRIRIGAAVVALALLTLSGVGGQLIGRLSEVQQNDSSRFLPK
                     SAESTRVSELATGFAVGEDSLPYFVVIERSAGLRPEDRARVTSYLASLPGLPIAPGSA
                     QTLDGYLAQTPGQAVPSKDGQAELVGVTIKADRLRETIGAKTVLYLAAEALREAARSQ
                     LAPHGLAVYVTGPGGVFADFVTAFGGIDSILLYVALGVVFFILLLVYRSPVLPFTVLL
                     SAGFGLSLAALVVFPLAKNGTLDLDGQSQGILSILVVGASTDYALLLVARFREELHDE
                     PNTWLAMTRAWRASLPPIAASAATVILGLLCLRLSDLGNTRGLGPVGAIGIAGAFVAS
                     LTFLPAVLVTVGRRIFWPRVPAMDHVHAADALGRRGLWGRVASLVGAHPRRVWVLTLA
                     GLLALAAFVPTLKADGVSQSDIFLTRVESVTGQEVLAAHFPAGSGSPAVILAPEGKAD
                     AVVQVLTATEGLGDVRVGELPGVPPKVVDGRVQVQATLVARADSAEAEDLVAELRTTL
                     DSVGTDVLVGGSTATSLDVRTSSERDLRVIAPAILAVIFLVLALLLRSLVASVILVAA
                     NVVSFAATVGAAAIAFNHVFKFPGSDPSTPLYAFVFLVALGIDYSIFLMTRVREETHV
                     RGTRGGILVGLAVTGGVITSAGIVLASTFAALAVLPILFLVQIAFLVAFGVLLDTLVV
                     RSLLVPALGHDIGSRIWWPSALTAKE"
     gene            3029..>4019
                     /locus_tag="VHM65_10240"
     CDS             3029..>4019
                     /locus_tag="VHM65_10240"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2706127.1"
                     /translation="MTRAGLVGYGMAGREFHAPLLARAGCVVAAVATRDPGRVAEVGA
                     AYPGAVVVPDLQALLALDDLDVVVLASPTGVHAEQAMACVAAGVAVVVDKPLAVSAPR
                     AAAVVAAAAEAGVPLTVFQNRRYDPEFATMRELVSSGVLGELIRAELRWERWRPVPKD
                     RWRENAAAAEGGGIMLDLHTHLLDQAVQLFGPVARVYAEVRAYTTRAEDEAFLACTHV
                     SGMSSHIGATSLSGAAGPRLRLLGRGGTYLLAVAGQEESLLTELGNDATHHGWLMRGS
                     ERERVQAQHADPVDFYRSVAAALGSADRQTAMPVDPADAVHVLAVIDAARTSAA"
BASE COUNT          487 a         1487 c         1428 g          617 t
ORIGIN      
        1 ggcctccgat gacggcatgc gcaactcctc cctgctcgtc aggaccgacg ctatcggcta
       61 cccggtatgc cgggcgctca cgacgcgcgg gcggccccgg cctggctcac cggcccgagc
      121 acgtccagca ggtgccgctc ggcgaggggg aggatctcgg ccaccgtgac gtcacggccc
      181 agctcgcggc tgatcgagga gacgccggcg tcgggaattc cacaggggat gatcgggtcg
      241 gcccaggcga ggtcgcagca cgcgttgagg gcgaacccgt gcatcgtgac gaaccggctg
      301 acccgtaccc cgatggcgcc gagcttgcgg tccggtccgc ggtggtcggc cggcacccag
      361 acgccgctgc gcccgtcgac ccgggccgcc gccagaccga ggtcggagca cacccggatc
      421 atgacctcct cgaggcgccg cacgtgggcg acgacgttga tcggcaccgg cagccgcacg
      481 atcgggtagc cgacgagctg gcccggtccg tgccacgtga tcttgccgcc acggtcgacg
      541 tcgacgacgg gggttccgtc ggtgggccgc tcgtgcggtt cggtgcgctt gccggccgtg
      601 tagacggcgg catgctcgag gagcagcacc gtgtcgggga tcgacccctc gacgaccccg
      661 gcgtggacct cgcgctgcag cgcccaggtc tgctcgtagt cggcgtagtc cggcgcgaag
      721 ccggcatggc ggatctgcat aagggaaggg tacgtcggga ggcggcacag gcaccggcga
      781 ggcggcaccg gcaccgtcct aggatgagcc gaccgacctc aaggcaggag ggacccacca
      841 tggaagcgac cccgcccggc acgggctcag cgcgacgtgc cccgcgtcgt gaccggatgc
      901 ccgtgcgcat ccggatcgga gcggccgtcg tcgcgctcgc gctgctgacg ctgagcgggg
      961 tcggtgggca gctcatcggg aggctgtccg aggtccagca gaacgacagc tcccgcttcc
     1021 tgccgaagtc ggccgagtcg acgcgggtca gcgagctcgc cacggggttc gccgtcggcg
     1081 aggactcctt gccgtacttc gtcgtcatcg agcgctcggc cggcctgcgg cccgaggacc
     1141 gggcgagggt gacgagctac ctcgcctccc tgcccggcct gcccatcgcc ccgggatcgg
     1201 cacagaccct cgacggctac ctggcccaga cccccggcca ggcggtgccg agcaaggacg
     1261 gccaggccga gctcgtcggg gtcaccatca aggccgaccg gctccgcgag acgatcggcg
     1321 ccaagacggt cctctacctg gccgccgagg ccttgcgcga ggccgcgagg tcacagctgg
     1381 ctccgcacgg tctcgcggtc tacgtcaccg gcccgggcgg ggtgttcgcc gacttcgtca
     1441 cggcgttcgg cgggatcgac tcgatcctgc tctatgtcgc cctgggcgtg gtcttcttca
     1501 tcctgctgct cgtctaccgc agcccggtgc tgccgttcac cgtcctgcta agcgcgggct
     1561 tcggactgtc cctcgcggcc ctggtcgtct tcccgctggc caagaacggc actctcgacc
     1621 tggacgggca gagccagggc atcttgtcga tcctcgtcgt gggggcgtcg accgactacg
     1681 cccttctcct cgtggcccgc ttccgcgagg agctgcacga cgagcccaac acgtggctgg
     1741 cgatgacgag ggcgtggcgg gcgtcgctgc cgcccatcgc ggcgagcgcg gcaaccgtca
     1801 tactcggcct gctctgcctg cggctctccg atctggggaa cacccgtggc ctgggcccgg
     1861 tcggggcgat cgggattgcc ggcgccttcg tcgcctcgct caccttcctg cccgcggtcc
     1921 tcgtcacggt gggccggcgt atcttctggc cgcgggtccc cgcgatggac cacgtccacg
     1981 ccgccgatgc tctcggccgg cgcgggctgt ggggccgcgt tgcctccctc gtcggagcgc
     2041 acccgcgccg ggtgtgggtc ctcaccctgg ccgggctgct cgcgctggcg gcgttcgtcc
     2101 ccacgctcaa ggccgacggg gtctcgcagt cggacatctt cctcaccagg gtcgagtcgg
     2161 tcaccggcca ggaggtgctc gcggcgcact tccccgcggg ctcgggctcg ccggcggtga
     2221 tcctcgctcc cgaaggcaag gccgatgcgg tcgtccaggt gctgacggcc accgagggtc
     2281 tcggggacgt ccgcgtcggt gagctccccg gcgtgccgcc caaggtcgtc gacggccggg
     2341 tccaggtcca ggccacgctc gtcgccaggg ccgacagcgc cgaggccgag gacctcgtcg
     2401 ccgagctcag gacgacactg gactccgtgg ggaccgacgt cctcgtcggc gggtcgaccg
     2461 cgaccagcct cgatgtccgc acgtccagcg aacgtgacct gcgggtcatc gcccccgcga
     2521 tcctcgccgt catcttcctc gtcctcgcgc tgctgctgcg ctcactcgtg gcgtcggtga
     2581 tcctcgtcgc ggcaaacgtc gtctccttcg ccgcgaccgt cggggcggcc gccatcgcct
     2641 tcaaccacgt gttcaagttc cctggctcgg acccgtcgac accgctctac gccttcgtct
     2701 tcctcgtcgc cctcgggatc gactactcga tcttcctcat gacccgggtg cgcgaggaga
     2761 cccacgtccg gggcacccgt ggcggcatcc tcgtcgggct ggccgtgacc gggggcgtca
     2821 tcaccagcgc cggcatcgtg ctggcgtcga ccttcgccgc cctcgccgtg ctgccgatcc
     2881 tcttcctggt tcagatcgcc ttcctcgtcg ccttcggcgt cctgctcgac accctcgtcg
     2941 tgcgcagcct gctcgtccct gccctcggcc acgacatcgg ctcgcggatc tggtggccgt
     3001 cggccctgac cgcgaaggag tgagcggtat gacgcgtgcg gggctggtcg ggtacggcat
     3061 ggccggccgg gagttccacg cgcccttgct cgcccgggcc ggctgcgtgg ttgccgcggt
     3121 ggcgacccgg gaccctggcc gcgtcgccga ggtgggcgcg gcataccccg gagccgtcgt
     3181 cgttcccgac ctgcaggcac tgctggcgct cgatgacctc gacgtcgtcg tcctcgcgtc
     3241 gcccacgggc gtgcacgccg agcaggcgat ggcctgcgtc gccgccggcg tcgcggtcgt
     3301 cgtcgacaag ccccttgcgg tcagtgcccc gagggccgct gcggtggtcg ccgccgcagc
     3361 cgaggcgggg gtgccactga cggtcttcca gaaccggcgc tacgacccgg agttcgcgac
     3421 gatgcgcgag ctggtgtcct cgggcgtcct cggtgagctc atcagggccg agctccggtg
     3481 ggagcgctgg cggcccgtgc ccaaggaccg gtggcgcgag aacgccgcgg cggccgaggg
     3541 cggagggatc atgctcgacc tgcacaccca cctgctcgac caggccgtgc agctcttcgg
     3601 gccggtggcc cgggtctacg ccgaggtgcg cgcctacacc acccgcgcgg aggacgaggc
     3661 gttcctcgcc tgcacccacg tgagcgggat gtcgagccac atcggtgcga cctcgctgag
     3721 cggggccgct gggccgcggc tgcgtctgct cgggcggggc ggcacctacc tgctggcggt
     3781 ggcgggccag gaggagtcgc tgctgaccga gctcggcaac gacgcgaccc accacggctg
     3841 gctgatgcgc gggtccgagc gggagcgggt ccaggcccag cacgccgacc cggtggactt
     3901 ctaccgatcg gtcgccgcgg cgctgggctc ggccgaccgg cagaccgcca tgccggtcga
     3961 ccccgccgat gcggtccatg tgctcgcggt catcgacgcc gcacggacca gcgctgccg
//
LOCUS       DATAIX010000696         4672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_296241, whole genome shotgun sequence.
ACCESSION   DATAIX010000696 DATAIX010000000
VERSION     DATAIX010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296241"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VHM65_10245"
     CDS             <1..655
                     /locus_tag="VHM65_10245"
                     /inference="COORDINATES: protein motif:HMM:NF023283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3375 family protein"
                     /protein_id="HEX2706128.1"
                     /translation="VRRIDHDWLRAADRTQATVRQLSDQLRRFLDDRVWLENRRVMDL
                     LRSIETSAMALRGAAVPLTVGLDALSPRVALPFERPLYSRPAQASLHSAVADGEDQAE
                     PESLFTQDHVDYQRVASSVRAALRHTRQVALTDLVDAHPLEQGLGEVMAYLGLSEDGV
                     EVVFDDDAIEELRWELDDGALRRAELPAVAYVRTDLPAGRAGGAAEGFPSGREGDRS"
     gene            652..1329
                     /locus_tag="VHM65_10250"
     CDS             652..1329
                     /locus_tag="VHM65_10250"
                     /inference="COORDINATES: protein motif:HMM:NF025205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HEX2706129.1"
                     /translation="MTVSPSRVVVADGRDLSKVVVTLLKGVLYESEQPSLWALLHRYR
                     PQVSDHMSVMGLDLVIDEAEGYAFLRQGTYDDADFPRLVPRHRLSFRVSLLLALLRKR
                     LAEFDASSGDPRLILHRDQVVELLRVHLPDAGNDVRLVADVETLISKVVDLGFLRRMT
                     GQESTVEVRRVLKAYVDAQWLADFDERLRDYLASAAGGGADPDALADRAGRGDRPGRA
                     LTGADDG"
     gene            1322..>4672
                     /locus_tag="VHM65_10255"
     CDS             1322..>4672
                     /locus_tag="VHM65_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HEX2706130.1"
                     /translation="MDDGELAGFRLDRVETLGWGTFDKSVWTFALHGRTSLLTGDIGS
                     GKSTLVDALTTLLLPAHRIAYNKAAGAETRERSLRSYVLGYYKSERSETTGTSRPVAL
                     RDHHSHSVVLAVFRHERLDQAVTLAQVFWLPDDRMGQPNRFYVVADHDLSIADDFADF
                     GSDIRGLRTRLRAQGITPIGAFPEYDRTFRRALGIPSTQAMDLFHQTVSMKSVGNLND
                     FVRGHMLEPFDAAESVRALVEHFKDLTAAHDAVIRARAQLAALDPIVTGCDEVDRITA
                     RLTRLREDQAAMQYAGARLAQRLLDAEREALDGEAAAAESALEAVEADLAGLRRREQG
                     LRDARAGHGGGRVGQLEQELERHNDNREDRAKRHRAFDEALGAAGLDALGDPARFGAA
                     LAQARDRHGATAEAIRTADREATDLGVTRRDLAVESGQIRDELRSLGGRRSSIPVAAL
                     GLRGRLCADLGLPEDDLPFVGELISVRPEQGRWTGAAERVLRGFALSLVVPDQHYDSV
                     SGWVDERHLGTRLVYHHVRPDRVGPVPPPEAGSLAAKLEIRPGHLAGWLAQDLATRAD
                     HQCVQSSAELRRARRAVTIGGQVKSGSRHEKDDRRPLDDASHHVLGWDNSAKVEALIR
                     RGTQVQQQLAEHDEESRRLTQRRGGLIARSEALAKVLAVDDVRDLDWWDSARRAAGVK
                     RELDQIRAGSAELARIEADLGATTTAITDAEASKGEHQRRLGGVRATLGRVQTGIRAA
                     AATLDRMPGELVDAVARAEESLGQRVVGTADEVPALIQEITAELVGALDARSRALTSA
                     QSRVEKSMAAFRLDYPAETTELDASMASAAEFRELRDRVAGDDLPRFEAEFKRYLNQN
                     AIREIAAFQARLGQQADVITERVRTINASLEDIDYNPDRYIRLEPQATPLIEIRQFRQ
                     DLRACTEDALAGDGDDLYSEAKFLQVEAIISKFTGREARTEADRAWTERVTDVRNWFV
                     FVASERWRHDDTEHETYADSDGKSGGQKEKLAYTILAASLAYQFRLDESDPLAKTFRF
                     AVIDEAFGRGSDQSTRYALRLFQRLGLQLLVVTPMQKVHTIEPFVERVGFVDNPTGAA
                     SRLHTLTISEYRAGRERVLASRSGAAAET"
BASE COUNT          663 a         1710 c         1670 g          629 t
ORIGIN      
        1 ggtgcgccga atcgaccacg actggctcag ggccgccgac cgcacccagg ccacggtccg
       61 ccagctgtcc gaccagctgc ggcgcttcct cgacgaccgg gtgtggctgg agaaccgtcg
      121 cgtcatggac ctcctgcgca gcatcgagac gagcgccatg gcgctgcgcg gcgcggcggt
      181 ccccctgacg gtggggctcg acgcgctcag cccgcgcgtg gcgctgccgt tcgagcgccc
      241 gctctacagc cggccggctc aggcgagcct gcacagcgcc gtggcggacg gcgaggacca
      301 ggccgagccg gagtcgctct tcacccagga ccatgtcgac taccagcggg tggccagctc
      361 ggtccgggcg gcgctgcgtc ataccaggca ggtcgccctg accgacctcg tcgacgcaca
      421 cccgctggag cagggcctgg gcgaggtcat ggcctacctc gggctcagcg aggacggggt
      481 cgaagtggtc ttcgacgacg acgcgatcga ggagctgcgc tgggagctcg acgacggcgc
      541 cctgcgccgg gccgagctgc cggccgtcgc ctacgtccgc acggacctgc cggccggccg
      601 ggccggtggc gcagccgagg ggttcccgag cgggcgcgaa ggggaccggt catgaccgtc
      661 tcgccctccc gggtggtcgt cgctgacggg cgcgacctgt ccaaggtcgt cgtcaccctg
      721 ctcaagggcg tgctctacga gagcgagcag ccatccctgt gggcgctcct gcaccgctac
      781 cggccccagg tcagcgacca catgtcggtc atggggctcg accttgtcat cgacgaggcg
      841 gagggctacg ccttcctgcg ccaggggacg tatgacgatg ccgacttccc gcgcctggtc
      901 ccccggcacc ggctgtcctt ccgggtcagc ctcctgctcg ccctgctgcg caagcggctg
      961 gccgagttcg acgcgtcgag cggtgacccg cgcctcatcc tgcaccgtga ccaggtcgtc
     1021 gagctcctgc gggtccacct gcccgatgcg ggcaacgacg tccggctcgt cgccgacgtc
     1081 gagacgctga tcagcaaggt cgtcgacctc ggtttcctgc gccggatgac cggccaggag
     1141 tccaccgtcg aggtccgccg tgtgctcaag gcgtatgtcg atgcgcaatg gctggccgac
     1201 ttcgacgagc gcctgcgcga ctacctggcc tcggccgcgg gtggtggtgc cgacccggac
     1261 gcccttgccg accgggccgg tcggggcgac cggcccggcc gcgcactgac aggagccgac
     1321 gatggatgac ggcgagctcg cgggcttccg cctcgaccgg gtcgagacgc tcggctgggg
     1381 caccttcgac aagtcggtgt ggacgttcgc cctccacggg cgcacctcgc tgctcaccgg
     1441 cgacatcggg tccggcaagt ccaccctggt cgacgcgctg acgacgctgc tgctgcccgc
     1501 ccaccgcatc gcctacaaca aggcagcggg tgccgagacc cgcgagcgca gcctgcggtc
     1561 ctacgtgctc ggctactaca agtcggagcg cagcgagacc accggcacct cacgcccggt
     1621 ggccttgcgc gaccatcact cccactcggt cgtcctggcc gtcttccgcc atgagcggct
     1681 cgaccaggcc gtcaccctgg cgcaggtctt ctggctcccg gacgaccgga tgggccagcc
     1741 caaccggttc tacgtcgtcg ccgaccatga cctgtcgatc gccgacgact tcgccgactt
     1801 cggcagtgac atccgtgggc tgcgcacgcg gttgcgcgcc cagggtatca ccccgatcgg
     1861 ggccttcccc gagtacgacc gcaccttccg ccgcgcgctc ggcatcccgt cgacgcaggc
     1921 catggacctg ttccaccaga ccgtgtcgat gaagtcggtg ggcaacctca acgacttcgt
     1981 ccggggtcac atgctcgagc cgttcgacgc ggccgagtcg gtccgcgctc tcgtcgagca
     2041 cttcaaggac ctcaccgcgg cccacgacgc ggtcatccgc gcccgcgcgc agctcgccgc
     2101 cctggacccc atcgtcacgg ggtgcgacga ggtcgaccgc atcacggccc ggctcacccg
     2161 cctgcgcgag gaccaggcgg ccatgcagta cgccggggct cgcctcgccc agcggctgct
     2221 cgacgccgag cgggaggccc tggacggcga ggcggccgcg gccgagtcgg cgctagaagc
     2281 ggtcgaggcg gatctggccg gcctgcgccg ccgggagcag ggtctgcgcg acgcccgggc
     2341 cggtcacggc ggtggccggg tcggccagct cgagcaagag ctggagcgcc ataacgacaa
     2401 ccgtgaggac cgggccaagc gccaccgtgc cttcgacgag gcgctggggg cggccggcct
     2461 ggacgcgctg ggagacccgg cgcgcttcgg tgccgccctg gcgcaggcgc gtgaccgcca
     2521 cggcgcgacg gcggaggcga tccggacggc cgaccgcgag gcgaccgacc tcggcgtcac
     2581 gcggcgcgac ctcgcggtcg agtcggggca gatccgagac gagctgcgca gcctgggtgg
     2641 tcgccgctcg agcatcccgg tggcggcgct ggggctgcgg ggccggctct gcgccgacct
     2701 cgggctgccc gaggacgacc tgcccttcgt cggtgagctg atctcggtgc gtcccgagca
     2761 gggccgctgg acgggggctg ccgagcgggt gctgcggggg ttcgccctgt cgctcgtcgt
     2821 gccggaccag cactacgaca gcgtctccgg gtgggtcgac gagcgccatc tcgggactcg
     2881 cctggtctac caccatgtcc gcccggaccg ggtcggaccg gtccccccgc ccgaggcggg
     2941 ctcgctcgcg gccaagctcg agatcaggcc cggccacctc gccgggtggc tggcgcagga
     3001 tctcgcgacc cgggccgacc accagtgtgt ccagtcctcg gccgagctgc gccgcgcccg
     3061 gcgcgccgtg acgatcggtg gtcaggtcaa gtccgggtcg cggcacgaga aggacgaccg
     3121 ccgtccgctc gacgacgcct cgcaccacgt cctcggctgg gacaactccg ccaaggtcga
     3181 ggcgctgatc cggcgcggca cccaggtcca gcagcagctc gccgagcacg acgaggagtc
     3241 ccgtcggctg acccagcgcc ggggtgggct gatcgcccgg tccgaggcgc tggccaaggt
     3301 cctcgccgtc gacgacgtcc gcgacctgga ctggtgggac agcgcccggc gagccgccgg
     3361 cgtcaagcgc gagctcgacc agatccgggc cgggtccgcc gagctggcgc gcatcgaagc
     3421 cgatctcggc gccacgacta cggccatcac cgacgcagag gcgagcaagg gcgagcacca
     3481 gcggcgcctc ggtggggtgc gggccacgct gggacgggtg cagaccggca tacgggcggc
     3541 tgctgcgacg ctggaccgca tgcctggaga gctcgtcgac gccgtcgccc gggccgagga
     3601 gtcgctcggg cagcgggttg tcgggaccgc ggacgaggtg ccggcgctca tccaggagat
     3661 caccgcagag ctggtcgggg cgctcgacgc ccggtcgcgg gccctcacct ccgcccagtc
     3721 gcgcgtcgag aagtccatgg cggcgttccg gctggactac cccgccgaga cgaccgagct
     3781 cgacgcgagt atggcgtcgg cggccgagtt ccgtgagctg cgcgaccggg tggccggtga
     3841 cgacctgcca cggttcgaag ccgagttcaa gcgctacctc aaccagaacg ccatccgcga
     3901 gatcgccgcc ttccaggccc ggctgggcca gcaggccgac gtcatcaccg agcgggtacg
     3961 cacgatcaac gcctcgctcg aggacatcga ctacaacccg gaccgctaca tccggctcga
     4021 accgcaggcg acaccgctca tcgagatccg ccagttccgc caggacctgc gcgcctgcac
     4081 cgaggacgcg ctggccggcg acggcgacga cctctactcg gaggcgaagt tcctccaggt
     4141 cgaggcgatc atctcgaagt tcaccgggcg ggaggcgcgc accgaggccg accgggcgtg
     4201 gaccgagcgg gtcaccgatg tgcgcaactg gttcgtcttc gtcgcctcgg agcggtggcg
     4261 ccacgacgac accgagcacg agacgtatgc cgactccgac ggcaagtccg gaggccagaa
     4321 ggagaagctc gcctacacca tcctcgcggc gtcgctggcc taccagttcc ggctcgacga
     4381 gagcgacccg ctggccaaga cgttccggtt cgcggtgatc gacgaggcgt tcgggcgtgg
     4441 gtcggaccag tcgacccggt atgcgttgag gctcttccag cggctcgggc tgcagctgct
     4501 cgtcgtcacg ccgatgcaga aggtccacac gatcgagccc ttcgtcgagc gggtcggctt
     4561 cgtcgacaac ccgacgggtg cggcgtcgcg gctgcacacg ctgacgatct cggagtaccg
     4621 cgccgggcgc gagcgggtcc tggccagccg gtccggcgcg gcagcggaga cg
//
LOCUS       DATAIX010000697         3305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_296266, whole genome shotgun sequence.
ACCESSION   DATAIX010000697 DATAIX010000000
VERSION     DATAIX010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296266"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1611
                     /locus_tag="VHM65_10260"
     CDS             <1..1611
                     /locus_tag="VHM65_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HEX2706131.1"
                     /translation="QRHAQRLVGMPVSAVTADGSPRASLTMGLWEPALKGPNDGPPRR
                     RSVIAETAELLADLVAADVQTVAFARSRAGVEVVAAMARERLERAHPAAVSRVAAYRG
                     GYLPEDRRDLERRLRDRSILGLAATNALELGIDVRGLDAVLLAGWPGTRASLWQQAGR
                     AGRAGSAALAVFVAADDPLDTYLVHHPEVLFDRPVEATVCDPGNPHILAPHLAAAAAE
                     VPITEADETYFGPAMVPLLDALAGAEILRRRPTGWYWARADRPTAHLTLRGAGEVVQI
                     VEASTGRVIGTVDEVAAHSQVHPGAVHLHQGQPFVVTDLDLVDRIALVTPGDPGWSTQ
                     SRSVSAFDIVTPEAEQAWGSVGCFFGTVAVRTQVTSFLRRRPTGEVLGEHPLDLPIRT
                     LDTKAVWWTFPTGVLEAAGLAAPDIPGAAHAAEHAAIGMLPLVASADRWDIGGVSTAL
                     HPDTGLPTILIYDGYPGGAGFAERGFGAAPAWLAATRDAIAACSCSRGCPSCVQSPKC
                     GNGNEPLDKAGALTILHVALAASVGSIA"
     gene            complement(2127..2465)
                     /locus_tag="VHM65_10265"
     CDS             complement(2127..2465)
                     /locus_tag="VHM65_10265"
                     /inference="COORDINATES: protein motif:HMM:NF041390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family type IV pilus minor pilin"
                     /protein_id="HEX2706132.1"
                     /translation="MATANSAVAIPALVLVLGLVLSAVSAGVDQIRCVDAARLAARAA
                     ARGDGPASVRALAAQAAPAGAVVTVSHGGERVHVVVTARRPLTPVSSLGFTVSASAEA
                     AVEGASGAPP"
     gene            complement(2494..2733)
                     /locus_tag="VHM65_10270"
     CDS             complement(2494..2733)
                     /locus_tag="VHM65_10270"
                     /inference="COORDINATES: protein motif:HMM:NF025395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HEX2706133.1"
                     /translation="MSSVTALRRRRTAHVAALARRGRMWSRRRGADAGMTTAEYAVGT
                     IAACSFALVLIAIVKSGPIRSALVRVITTALGIAG"
     gene            complement(2906..3061)
                     /locus_tag="VHM65_10275"
     CDS             complement(2906..3061)
                     /locus_tag="VHM65_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706134.1"
                     /translation="MRADDTARADEAAARLGVRLVLPLGLAFLPAFGLTTVVPIVVAL
                     AGQLLSG"
     gene            3115..>3305
                     /locus_tag="VHM65_10280"
     CDS             3115..>3305
                     /locus_tag="VHM65_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX2706135.1"
                     /translation="MATTLTLLSLAVRGGASVIEALERVARVSADPVRAHLATVAAAL
                     RWGLSATDAWSCVPSAWTPA"
BASE COUNT          424 a         1268 c         1178 g          435 t
ORIGIN      
        1 caacgccacg cccagcggct cgtcggtatg ccggtcagcg cggtcactgc cgacgggtcg
       61 cctcgggcga gcctcaccat gggtctgtgg gagcccgccc tcaaggggcc caacgacgga
      121 cccccacggc gacgcagcgt catcgccgag accgccgagc tgctcgcgga cctcgtggcc
      181 gccgatgtcc agacggtcgc gtttgcccgg tcgagagccg gcgtcgaggt cgtcgccgcc
      241 atggcccggg aacgccttga gcgcgcccac cccgccgcgg tctcgcgggt cgcggcatac
      301 cgcggtggct atctgcccga ggaccgacgc gacctcgagc gccggctgcg cgaccgctcg
      361 atcctcggcc tcgcagcaac gaacgccctc gagctcggca tcgacgtccg tggcctcgac
      421 gccgtcctgc tcgcgggctg gccgggcacc cgcgcgtcgc tctggcagca ggccggccgg
      481 gcaggccggg cgggctctgc cgcgctcgcg gtgttcgttg cggctgacga ccccctcgac
      541 acctacctcg tccatcaccc cgaggtgctc ttcgaccgcc cggtcgaggc caccgtgtgc
      601 gacccgggca acccgcacat cctcgcgcct cacctggcgg ccgcggccgc agaggtcccg
      661 atcaccgagg cggacgagac ctacttcgga ccggcaatgg tgcccctgct cgatgcgctc
      721 gccggagccg agatcctgcg ccgtcggccg accgggtggt actgggcccg ggcggaccgc
      781 cccacagcgc acctcaccct gcgcggggcc ggggaggtcg tccagatcgt cgaagcgagc
      841 acgggccgag tcatcgggac cgtcgacgag gtggccgcgc acagccaggt ccaccccggc
      901 gccgttcacc tccaccaagg ccagccgttt gtcgtcaccg acctcgacct ggtcgatcgg
      961 atcgctctgg tgactccggg tgaccccggg tggtcgaccc agtcccggtc ggtgagtgcc
     1021 ttcgacatcg tgacaccgga ggccgagcag gcctgggggt cggtggggtg cttcttcggc
     1081 accgtggccg tacgtaccca ggtcacctcc ttcctgcggc gacggcccac cggagaggtc
     1141 cttggcgagc acccgctcga cttgcccatc cgcaccctgg acacgaaggc ggtctggtgg
     1201 accttcccga ccggggtgct cgaggcggcc ggcctggctg ccccggacat tcccggcgcc
     1261 gcccacgccg ctgagcacgc cgccatcggc atgctccccc tcgtcgcgag cgccgaccgg
     1321 tgggacatcg gcggtgtctc gacggcgctg caccccgata ccggcctgcc gacgatcctc
     1381 atctatgacg gctaccccgg cggcgccggc tttgccgaac gcggcttcgg cgcggcgccc
     1441 gcgtggcttg cggcaacccg cgacgccatc gccgcctgta gctgctcccg gggatgcccc
     1501 tcctgcgtcc agtcccccaa gtgcggcaac gggaacgagc ccctcgacaa ggccggcgcc
     1561 ctcacgatcc tgcacgtggc actcgcggcc agcgtggggt ccatcgcctg agaacgccgc
     1621 ggctacgccg tacccccagc ggtgtgagca cccgagcgcc cgggcggtcc cgcccgggcc
     1681 ggcccgggct tgcgctcggg ccctccagcc accgccaggg aagccctcgg cgagcgccac
     1741 ctcggccact gcctgaacga ggaccaccgc gccgtctacc cggcaggctg cgacccgcgt
     1801 gccgttgccg gcggcgatcg cccgtgcgac atcgcacgcc tcggccgggc tcgaccccga
     1861 ctgcagcgcc tgggcagccg ccagcgcggc gagatcagcg cccgagcgcg cccggtgggt
     1921 ggccgcgacg acgcccgcga gggaaagcgc accgaccgcg acgaggatca gcgcaccgat
     1981 cgcggccgct gccgcgaccg tcatcccgcc gcggtcgtga cgctgggctc gccggggcgc
     2041 ccgtcggctg tcagcgcgcc cgcaggctcc agccacccgc ctgcgtgccc aagccgcccg
     2101 gacgtccagg accagtcttt gggggatcac ggtggcgctc ctgacgcccc ctcgacggct
     2161 gcctcggcgc tggcgctcac ggtgaaaccc aggcttgaga cgggggtcaa cggccgtctg
     2221 gccgtgacaa ccacatgcac tcgctcacca ccatggctca cggtcacgac cgccccagcc
     2281 ggggcagcct gggcggccag cgcccgcacc gacgccgggc cgtcaccgcg cgcggctgcg
     2341 cgggcagcca gccgggccgc gtcgacacag cggatctggt cgacgccggc gctgaccgcg
     2401 gagagcacca gcccgagcac gaggaccagt gcagggatgg ccacggccga gttcgccgtg
     2461 gccatccccc ggtcacctcg aggggtcacc ggctcagcct gcgatcccga gggcggtcgt
     2521 gatcactcgt acgagtgccg agcggatcgg cccggacttc acgatggcga tgaggacgag
     2581 agcgaagctg cacgcggcga tcgtgccgac ggcatactcc gccgtcgtca tccccgcgtc
     2641 ggcaccgcga cggcgcgacc acatacggcc tcgccgggcc agcgctgcca cgtgtgcggt
     2701 ccgccgtcgg cgaagggcag tcaccgagct catgggggtc tccttccgtg cgccgtgtgg
     2761 cgcgttccgc gagcgcccgg atggcgctcg cgcgaggggt tcgtgctggt gctcgcgcga
     2821 ggggtcgtgc tggtgctcgc gcgagggctt cgtgctgacg gtgctggcgg gccgggtgag
     2881 ggctgagtgc tcgctcatcg cgccctcatc ccgagaggag ctggcccgcg agggcgacga
     2941 cgatggggac cacggtggtg agaccgaagg ccgggaggaa cgccagcccc aacgggagga
     3001 cgaggcggac accgagccgc gccgctgcct cgtccgctcg agcggtgtca tcggcgcgca
     3061 cctgggcggc ggcgtcggcg agcagcccgg ccggtgggtc tgacccttga tgacgtggcg
     3121 accacgctga cgctgctgtc gctggctgtc cgtgggggtg cgtccgtgat cgaggcactc
     3181 gagcgggtgg cccgggtcag cgcggacccg gtgcgggccc atctggccac ggtggcggcc
     3241 gcgcttcgat gggggctctc ggcgaccgat gcctggtcat gcgttccctc ggcgtggacc
     3301 cccgc
//
LOCUS       DATAIX010000698         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_206107, whole genome shotgun sequence.
ACCESSION   DATAIX010000698 DATAIX010000000
VERSION     DATAIX010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206107"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1820
                     /locus_tag="VHM65_10285"
     CDS             <1..>1820
                     /locus_tag="VHM65_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX2706136.1"
                     /translation="GLPLDGWELAIVTESGELVEQGGQGELVIGGVGLARYLDPAKDA
                     EKFAPLPLLGWDRAYRSGDLVRHDPAGLVFVGRADEQVKLGGRRIELGEVDAALIALP
                     GVAGGAAAVRRTPAGNQILVGYVVPHDGAALDLAKARHTLGQVLPAALVPLLAAVADI
                     PTKTSGKVDRAALPWPLEGVGAGSDELGADLQGTARWLAGQWRSVLGTAPTSPDDDFF
                     SLGGQSLSAAQLVSVLRERYPDMTVADAYDNPRLRDQAVFLDSIGGPGAGMDNRSTRV
                     VQPVPRRSRVAQLLIGIPLSALTGLQWLSLLAAANNLVQAATDVPWAPTAPWWAVIVA
                     WLVLITPVGRMAISAIGARLLLRGLEPGSYPRGGHVHLRLWTAERLAEAVGAASMAGA
                     PWIIYYARALGATVGSGVDLHTLPPVTGMLTLGDGCSIEPEVDLAGHWLDGDQVHIGR
                     VWVGPGATVGGRSTLGPGTRIGAGAEISPGSGVSGKVPAGELWTGSPAVRQGKAGTEF
                     PTQRPARAVHWVAGYGVSSVLLAGVPLVSALPALWLLLHAAAGRSSLGSALVAASVWT
                     PVATLAGLTTLALLTILAVRLLGLGVRPGYHPVRRRVGFQV"
BASE COUNT          192 a          735 c          640 g          253 t
ORIGIN      
        1 ggcctcccgc tcgacggctg ggagctcgcg atcgtcaccg agtccggcga gctcgtcgag
       61 cagggcggtc agggcgagct cgtcatcggc ggggtcgggc tcgcgcgcta tctcgaccca
      121 gccaaggacg cggagaagtt cgccccgctg cccctgctcg gctgggaccg ggcctaccgc
      181 tccggcgacc tcgtccgcca cgacccggcc gggctcgtct tcgtcgggcg cgccgacgag
      241 caggtcaagc tcggcggccg gcgcatcgag ctcggcgagg tcgacgccgc cctcatcgcc
      301 cttcccggtg tcgccggtgg cgcggctgcg gtccgccgca ccccggccgg caaccagatc
      361 ctcgtcgggt atgtcgtccc gcacgacggc gccgccctcg acctcgccaa ggcgcgccat
      421 accctcggtc aggtcctccc agccgccctc gtgccgcttc tcgcggccgt ggcggacatc
      481 cccaccaaga cctccggcaa ggtcgaccgg gccgcgctgc cgtggccgct cgagggcgtc
      541 ggggcgggca gcgacgagct cggcgccgac ctgcagggca cggcgcggtg gctcgccggg
      601 cagtggcgct cggtcctcgg caccgctccc accagcccag acgacgactt cttcagcctc
      661 ggcgggcaga gcctctcggc cgcccagctc gtctcggtcc tgcgcgagcg ctaccccgac
      721 atgacggtcg ccgacgccta cgacaacccg aggctgcgcg accaggccgt cttcctcgac
      781 tcgatcggcg gccccggcgc gggcatggac aaccggagta cccgagtggt ccagccggtc
      841 ccgcgccgaa gccgagtcgc ccagctgctc atcgggattc cgctgtcggc gctgaccggg
      901 ctgcagtggc tgtccctgct tgccgccgcc aacaaccttg tccaggccgc caccgacgtc
      961 ccgtgggcgc cgaccgcccc gtggtgggcc gtcatcgtgg cctggctcgt cctcatcacc
     1021 ccggtggggc ggatggcgat ctccgccatc ggagcccggc tgctgctgcg cggcctcgag
     1081 cccggcagct acccccgggg cggccacgtc cacctgcggc tctggaccgc ggagcgtctc
     1141 gccgaggccg tcggagcggc gagtatggcg ggcgccccct ggatcatcta ctacgcccgc
     1201 gcgctcggcg ccacggtcgg gtcgggtgtc gacctgcaca cgctgccgcc ggtcaccggg
     1261 atgctcaccc tcggcgacgg ctgctcgatc gagcccgagg tcgacctcgc cgggcactgg
     1321 ctcgacggcg accaggtcca catcggccgg gtctgggtcg gacctggcgc caccgtcggc
     1381 gggcgcagca cgctgggccc cggcacccgg atcggcgcgg gcgccgagat cagccccggg
     1441 tccggcgtct cgggcaaggt gcccgccggc gagctctgga ccggttcgcc cgcggtccgc
     1501 cagggcaagg ccggcaccga gttccccacg cagcggccgg cccgggcggt gcactgggtg
     1561 gccgggtatg gcgtctcctc cgtcctgctc gccggcgtcc cgctcgtctc ggccctgcct
     1621 gccctctggc tcctcctgca tgccgccgcg ggccgcagct cgctcgggag cgccctcgtc
     1681 gcggccagcg tctggacccc ggtcgcgacg ctggccgggc tgaccacgct cgccctgctg
     1741 acgatcctcg cggtccggct gctcggcctc ggggtccggc ccggctacca ccccgtgcgc
     1801 cgccgggtcg gcttccaggt
//
LOCUS       DATAIX010000699         2373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_236160, whole genome shotgun sequence.
ACCESSION   DATAIX010000699 DATAIX010000000
VERSION     DATAIX010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236160"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..654
                     /gene="rpsP"
                     /locus_tag="VHM65_10290"
     CDS             163..654
                     /gene="rpsP"
                     /locus_tag="VHM65_10290"
                     /inference="COORDINATES: protein motif:HMM:NF011093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX2706137.1"
                     /translation="MAVKIRLKRMGKIRAPFYRVVVMDSRSRRDGRAIEEIGKYHPTE
                     EPSLIDISSERAQYWLGVGAQPSEQVQSLLKVTGDWQRFKGEDGTAGTLRAQRPKVSK
                     KDLYEAALAAAGKSIDAKGAATTARKRAARPEAAETAPAPQTGDAPAETAPAAADSPK
                     AEA"
     gene            657..896
                     /locus_tag="VHM65_10295"
     CDS             657..896
                     /locus_tag="VHM65_10295"
                     /inference="COORDINATES: protein motif:HMM:NF002761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX2706138.1"
                     /translation="MLEEALEHLVKGIVDHEDDVAVRRKQLRRGEILEVRVHPEDLGR
                     VIGRGGRTASALRTVVGALANDASVRVDIVDTDRR"
     gene            1017..1541
                     /gene="rimM"
                     /locus_tag="VHM65_10300"
     CDS             1017..1541
                     /gene="rimM"
                     /locus_tag="VHM65_10300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX2706139.1"
                     /translation="MKLVARIGKPHGLRGEVTVQSHTDDPDARFATGTTYRTDAETGS
                     GVPRELTLRSARLHNGTWLLAFEAIPDRVGAEGLRGARLHLDVAPGAPREDENGWYDE
                     QLVGLRAQDPSGQRLGVVTGIEHGPGQDRLVLDLEAGYPAYVPLVHELVPVVDPGAGY
                     VVVDAPPGLLELNA"
     gene            1551..>2373
                     /gene="trmD"
                     /locus_tag="VHM65_10305"
     CDS             1551..>2373
                     /gene="trmD"
                     /locus_tag="VHM65_10305"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX2706140.1"
                     /translation="MRIDVVTIFPGYLAPLGLSLIGKARRDGVLDVHVHDLRAFTHDR
                     HRTVDDSPYGGGAGMVMRPEPWGEALDHVRAQVPGAVPRLVVPAPSGTPFTQGMAREL
                     AAEPWLGFACGRFEGIDERVFDEAASLMPVSVVSLGDYVLGGGEVAVLAMVEAVARLV
                     PGVLGNAESLVEESHEDGLLEYPVFTKPAVWRGRPVPEVLRSGDHGAIAAWRHEQRLA
                     RTAARRPDLLHASAALGVADLAVETARPGDAGELMTLQRACWVQEGIASSTVQIPA"
BASE COUNT          334 a          882 c          841 g          316 t
ORIGIN      
        1 tcccgggact ggctgaatct ctggcacaat gggctgctgt acgcgtctgg cagggaccct
       61 ctcacccggt cggaacgtgt ccatccgaga ctcgccctcc gcagacccca cgcggcgcgg
      121 gcgtgcgctc gacccgaaac agactgagga gacaccacac ccgtggccgt caagattcga
      181 ctcaagcgca tgggcaagat ccgtgcaccg ttctaccgcg tcgtcgtcat ggactcccgc
      241 tcacgccggg atggccgggc catcgaggag atcggcaagt accatccgac cgaggagccg
      301 tcgctcatcg acatcagctc cgagcgggcg cagtactggc tcggcgtcgg cgcgcagccg
      361 agcgagcagg tccagagcct gctcaaggtc accggcgact ggcagaggtt caagggtgag
      421 gacggcacgg ccggcaccct gcgagcccag cggcccaagg tgagcaagaa ggacctctac
      481 gaggccgcac tggccgctgc cggcaagagc atcgacgcca agggtgccgc gacgaccgcg
      541 cgcaagcgcg ccgccaggcc cgaggcggcc gagacggccc cggctccgca gaccggtgac
      601 gccccggccg agaccgcccc tgcggccgcc gacagcccga aggccgaggc ctgagcatgc
      661 tggaggaggc gctcgagcac ctcgtcaagg gcatcgtcga ccacgaggac gacgtcgccg
      721 tccgccgcaa gcagctgcgc cggggcgaga tcctcgaggt gcgcgtccac ccggaggacc
      781 tcggccgggt gatcggccgc ggtggccgga ccgccagcgc gctgcgaacg gtcgtcggcg
      841 cgctcgccaa cgacgcgtcg gtgcgggtcg acatcgtcga caccgaccgt cgctgaggct
      901 cgactgccct ggctcgtcgc ggtatccacg tcgcgcaccc gacgcgccgg accggccctg
      961 agcatccgct cggcgcttcc cccggcgcgt cggcctttct ccccaggacc ttgcccatga
     1021 agctcgtcgc ccggatcggc aaaccccacg gcctgcgcgg tgaggtgacc gtccagtccc
     1081 acaccgacga ccccgacgcg cggttcgcga ccggcacgac ataccggacc gacgccgaaa
     1141 ccgggtcggg agtgccgcgc gagctcactc tgcgctcggc ccggctgcac aacgggacct
     1201 ggctactcgc cttcgaggcc atccccgacc gcgtcggtgc cgaaggcctg cggggcgccc
     1261 gcctgcacct cgacgtcgcc ccgggcgcgc cccgggagga cgagaacggc tggtatgacg
     1321 agcagctcgt gggtctgcgg gcacaggacc cgtccggaca gcgcctcggc gtggtcacgg
     1381 ggatcgagca cgggccgggc caggaccgcc tcgtcctgga cctcgaggcc ggctacccgg
     1441 catacgtccc gctcgtccac gagctcgtcc cggtcgtcga ccccggcgct gggtacgtcg
     1501 tcgtcgacgc cccgcccggg ctgctcgagc tcaacgcgta ggagccggcc gtgcgcatcg
     1561 acgtcgtgac gatcttcccg ggctaccttg ccccgctggg gctctcgctc atcggcaagg
     1621 cgcgccgcga cggggttctc gacgtgcacg tccacgacct gcgcgcgttc acccacgacc
     1681 ggcatcgcac ggtcgacgac agcccgtatg gcgggggggc gggcatggtc atgcgtcccg
     1741 aaccgtgggg tgaggccctc gaccacgtgc gtgcccaggt gccgggtgcc gtcccccggc
     1801 tcgtcgtgcc ggcgccgtcg ggcacaccct tcacgcaggg gatggcccgc gagctggcgg
     1861 ccgagccctg gctcggcttc gcctgtggtc gctttgaggg gatcgacgag cgggtgttcg
     1921 acgaggccgc ctcgctcatg cccgtgtcgg tcgtctcgct cggggactac gtcctgggcg
     1981 gcggcgaggt tgccgtcctc gccatggtcg aggccgtcgc gcggctcgtc ccgggggtcc
     2041 tcggcaatgc cgagtcgctc gtcgaggagt cgcacgagga cggcctgctg gagtaccccg
     2101 tcttcaccaa acctgcggta tggcgcggcc gcccggtccc cgaggtgctg cgctcgggcg
     2161 accacggcgc gatcgcggcg tggcgtcacg agcagcggct cgctcgcacg gcggcccggc
     2221 ggcccgacct gctccatgcg tcggcggcgc tcggtgtggc tgatctcgcg gtcgaaacgg
     2281 cccggcccgg tgacgccggc gagctcatga cgctgcagcg ggcgtgctgg gtccaggagg
     2341 gcatcgccag cagcacggtg cagatcccgg ccc
//
LOCUS       DATAIX010000700         2515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_206240, whole genome shotgun sequence.
ACCESSION   DATAIX010000700 DATAIX010000000
VERSION     DATAIX010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206240"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VHM65_10310"
     CDS             <1..607
                     /locus_tag="VHM65_10310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2706141.1"
                     /translation="STTRPPEPPSLTALVTAAADGDHHAWKALVDRFAPLVWAICRGF
                     RLPDPEAADVTQTVWLTAIEKLSTVREPEAFPGWIVTTTRRECLRVIERRAKDAPSID
                     MSEFDFGADPDQTAPDADLLAAERRAVVRAGFAQIPENCQKLLALVVMADEVSYAQIS
                     AKLNIPTGSIGPTRSRCLDKLRATPVMSVWLRSQSFEGKDR"
     gene            607..1062
                     /locus_tag="VHM65_10315"
     CDS             607..1062
                     /locus_tag="VHM65_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706142.1"
                     /translation="MTTPHLPSDAELLDLLREGLTTRDDVPASVYDAGYAAYSWRTID
                     VELAALMYDSAADLTGSGARSEQAAVRAMTFTTAVVTIEIEIGPGEVLGQLNPGMARQ
                     VTVEVQDSGPSVYPVNEVGGFVISPLPSSPFRLLVEVEGHTVVTGWVSR"
     gene            complement(1156..1416)
                     /locus_tag="VHM65_10320"
     CDS             complement(1156..1416)
                     /locus_tag="VHM65_10320"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX2706143.1"
                     /translation="MAQVHPEELGAVINRLKRAQGQIGGILRMIEESRDCKDVVTQLA
                     AVSRAVDRAGFAIIAQGLRECVLDPDDTVDTGDLEKLFLSLA"
     gene            complement(1433..2050)
                     /locus_tag="VHM65_10325"
     CDS             complement(1433..2050)
                     /locus_tag="VHM65_10325"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2706144.1"
                     /translation="MDPVDWDERYRHTDLMWGAEPNRWVEQWAAALAPGRALDLAAGE
                     GRNAIWLASRGWDTLAVDFSAVAVDRARTLAGASLGPGAGRLRAEVRDVVTWVPPGSA
                     FDLVVLCYLHLPPSERLAAVRAAAGAVARGGRLCVVGHDSANLAEGVGGPQDLTVLFT
                     PADIVDDLAGTGMSITHARTERRPVQTETGAELTALDAVVVATRP"
BASE COUNT          367 a          937 c          852 g          359 t
ORIGIN      
        1 gtccaccact cgcccgcccg agccgccctc gctgaccgcc ctcgtcaccg cggccgcgga
       61 cggtgaccat cacgcctgga aggccctcgt cgaccggttc gcacccctcg tgtgggccat
      121 ctgccggggc tttcgcctgc ccgaccccga ggccgccgac gttacccaga ccgtgtggct
      181 gacggcgatc gagaagctgt ccacggtccg cgagcccgag gcctttcctg gctggatcgt
      241 cacgacgacc cggcgcgagt gcctgcgggt catcgagcgc cgggccaagg acgccccctc
      301 gatcgacatg agcgagttcg acttcggcgc cgacccggac cagaccgctc cggatgccga
      361 cctgctcgcc gccgagcgcc gggcggtcgt ccgggccggc ttcgcccaga tcccggagaa
      421 ctgccagaag ctgctcgccc tcgtggtcat ggccgacgag gtctcctacg cgcagatcag
      481 cgccaagctg aacatcccca ccggcagcat cggaccgacc cggtcccgtt gcctcgacaa
      541 gctgcgcgcg accccagtga tgtccgtctg gctccggagc cagtcgttcg aaggaaaaga
      601 ccgatgatga cgactccgca cctgcccagc gacgccgagc tgctcgacct cctgcgcgag
      661 gggctgacca cccgagacga tgtgccggcc agcgtctacg acgcgggcta cgccgcgtac
      721 tcctggcgca ccatcgacgt cgagctcgcc gcgctcatgt acgactccgc cgccgacctg
      781 accggcagtg gcgcgcgctc ggagcaggct gccgtgcggg cgatgacgtt cacgaccgcc
      841 gtggtcacca tcgagatcga gatcggtccg ggcgaggtcc tcggccagct caacccgggt
      901 atggcgcgcc aggtcaccgt ggaggtgcag gactccgggc cgagcgtcta ccccgtcaac
      961 gaggtcggcg ggttcgtcat ctcgccgctg ccgtcctcac cgttccgcct gctcgtcgag
     1021 gtcgagggcc acaccgtcgt caccggctgg gtcagccgct aacagccccg gccgagccgg
     1081 ccctgcctcg gcccccaccg gggggagcgt cgcggggttc gtcaggcaag agagttcgtc
     1141 aggcacgaga gttcgtcagg caagagacag gaagagcttc tcgaggtcgc ccgtgtcgac
     1201 ggtgtcgtcg gggtcgagga cgcactcgcg cagtccctgg gcgatgatgg cgaacccggc
     1261 gcggtcgacg gcccggctca ccgcggcgag ctgggtcacc acgtccttgc agtcgcggct
     1321 ttcctcgatc atccgcagga tgccgccgat ctggccctgg gcgcgcttga gccggttgat
     1381 gacagcaccg agctcctcgg ggtgaacctg agccatggtc tctcctcgca agtcagggac
     1441 gggtggcgac gacgaccgcg tcgagggcgg tgagctcggc tccggtctcg gtctggacgg
     1501 gacggcgctc ggtccgggcg tgggtgatcg acatcccggt gccagcgagg tcatcgacga
     1561 tatcggccgg ggtgaacagc accgtcaggt cctgcgggcc gccgaccccc tcggcgaggt
     1621 tggcgctgtc atggccgacg acgcacagcc gcccaccgcg cgcgaccgcc cccgccgccg
     1681 cgcgcaccgc ggcgagtcgc tcgctgggcg gcaggtgcag gtagcagagc acgacgaggt
     1741 cgaaggcgct gcccggcggc acccaggtca cgacatcgcg gacctcggcg cgcaggcgtc
     1801 cggcgcctgg accgagcgag gcgccggcca gtgtccgggc ccggtccacc gcgacggcgg
     1861 agaagtcgac ggcgagcgtg tcccaccctc gggaggccag ccagatcgcg ttgcgccctt
     1921 cgccggcggc caggtcgaga gcgcgtccag gggccagcgc ggccgcccac tgctcgaccc
     1981 agcggttcgg ctcggctccc cacatcaggt ccgtatggcg gtagcgctcg tcccagtcga
     2041 cgggatccac acgtcctcct caacgttctc ggtactcggc gcccggcgac gccagccacc
     2101 gctcactata cgggcggggg tatcacacgt catacgggcg gggcatcaca cgtcatgccc
     2161 gcgcccggca cctgcacgca gccctgcgcc cgcaccctgg ctgcggcata ccggcacctc
     2221 acccggaccg caccacgagg cccagccggc acagacgccg tgggtctcaa cgccgcgtgg
     2281 cacgcactgg cacccgacgc gcagcacccc cacccggagc actcggttgc gcacagcgcc
     2341 agcagcgctg ggcgtgtgcc gccggttcac tgcaacagct cagcttgcga ttaaaccccc
     2401 gctatgcggc cttcttggtc gcgggatagc gggtgcgtgg ctctcggcgg gtgcctttgg
     2461 ggcgtcccgg gccggggctc gagctttatt gatgccgctt actgaaattc cccag
//
LOCUS       DATAIX010000701         1772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_206246, whole genome shotgun sequence.
ACCESSION   DATAIX010000701 DATAIX010000000
VERSION     DATAIX010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206246"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VHM65_10330"
     CDS             <1..438
                     /locus_tag="VHM65_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706145.1"
                     /translation="ALTRLGGGPPSTLWVAAVSLAALVLAGAAAWRHRACDLVVLVAA
                     ALAGLCVSPISWSHHWVWLAPAAVALVHLRHWAWAVLTAAVAVVGPHWLVPHDSAGLV
                     DPTPWSQAAAAAFPVLAACLLAVLAATPGGLAPHRRDGKHCGT"
     gene            435..>1772
                     /locus_tag="VHM65_10335"
     CDS             435..>1772
                     /locus_tag="VHM65_10335"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HEX2706146.1"
                     /translation="MSEPTYRTVFEPFRIHSVEPLRMTTAAYRADKAREASYNLFQVH
                     ADDVLIDLLTDSGTGAMSRDQWAAIQHGDESYAGSPSFFIFQDAVRELFPYEHIIPTH
                     QGRAAERILFQVLGGAGQVVPNNTHFDTTRANIEFTGAEAVDLVIAEGLNPRSNHPFK
                     GNMDTARLEALLTERGEQIPCVMMTITNNSGGGQPVSLENLREVSRIAHAHGKPLFLD
                     ACRFAENAWFIRLREPGQGERDVPDIVRDMAALADGMTMSAKKDPMGNIGGWLALNDD
                     ELAMQCRNLLILTEGFPTYGGLAGRDLEALAQGLKEVVSHDYLTYRIRSTAYLADALA
                     GLGVPVLTPAGGHAVYVDARTLLPHLGPLQYPGQSLAVALYETGGIRSCEIGTVMFGR
                     HPDGSEVPATMDLVRLAIPRRTYTQSHIDYVIEVFEQVVAQADALPAYRIAWEA"
BASE COUNT          250 a          668 c          590 g          264 t
ORIGIN      
        1 gccctcaccc ggctgggcgg cgggccacca tcgaccctgt gggttgcggc ggtctcgctg
       61 gccgcgctcg tcctggccgg agccgccgcc tggaggcacc gcgcctgcga cctcgtcgtc
      121 ctcgtcgccg ccgcgctcgc cggcctgtgc gtctcgccca tttcctggtc gcaccactgg
      181 gtctggctgg ctcccgccgc cgtggccctc gtgcacctgc gccactgggc ctgggctgtc
      241 ctcaccgccg cggtcgccgt cgtcggtccg cactggctcg tcccgcacga ctcggcgggt
      301 ctggtcgacc cgacgccatg gtcccaggca gcggccgccg ccttccccgt cctcgctgcc
      361 tgcctcctcg ccgtcctcgc cgcgacaccg gggggcctag cgccgcaccg ccgtgatggg
      421 aaacactgcg gcacatgagc gaaccgacat accggaccgt cttcgagccc ttccggatcc
      481 actccgtcga gccgctgcgg atgaccaccg cggcctaccg cgccgacaag gcgagggagg
      541 cgtcatacaa cctgttccag gtccacgcgg acgacgtcct catcgacctg ctcaccgact
      601 cgggcaccgg cgcgatgagc cgcgaccagt gggccgcgat ccagcacggg gacgagtcgt
      661 atgccgggtc gccgtcgttc ttcatcttcc aggacgcggt gcgtgagctg ttcccctacg
      721 agcacatcat cccgacgcac cagggccggg cggccgagcg gatcctgttc caggtgctcg
      781 gcggggccgg gcaggtcgtg ccgaacaaca cgcacttcga cacgacccgg gccaacatcg
      841 agttcaccgg cgccgaggcg gtcgacctcg tcatcgccga ggggctcaac ccccggagca
      901 accacccctt caagggcaac atggacacgg cccggctcga ggcgctgctc accgagcgcg
      961 gcgagcagat cccgtgcgtc atgatgacga tcaccaacaa ctccggcggc gggcagccgg
     1021 tctcgctgga gaacctgcgc gaggtgagcc ggatcgccca cgcgcacggc aagccgctct
     1081 tcctcgacgc gtgccgcttc gcagagaacg cgtggttcat ccggctgcgc gagccgggcc
     1141 agggcgagcg ggacgttccc gacatcgtcc gcgacatggc cgctctcgcc gacggcatga
     1201 cgatgagcgc caagaaggac ccgatgggca acatcggcgg ctggctcgcc ctcaacgacg
     1261 acgagctggc catgcagtgc cgcaacctgc tcatcctcac cgagggcttc cccacgtatg
     1321 gcgggctggc cggccgcgac ctcgaagcgc tcgcgcaggg gctcaaggag gtcgtctcgc
     1381 acgactacct cacgtatcgc atccgctcga cggcgtacct cgccgacgcg ctcgccggcc
     1441 tcggggtgcc ggtcctcacc cccgctggcg ggcatgcggt gtatgtcgac gcgcggacgc
     1501 tgctgcccca cctcgggccg ctgcagtacc cgggccagtc gctggcggtg gcgctctacg
     1561 agaccggcgg gatccgctcc tgtgagatcg ggacggtgat gttcgggcgg cacccggacg
     1621 gttcggaggt gcctgcgacg atggatctcg tccggctggc gatcccgcgg cggacgtata
     1681 cgcagagcca catcgactac gtcatcgagg tcttcgagca ggtcgtcgcg caggctgacg
     1741 cgctcccggc ataccggatc gcctgggagg cg
//
LOCUS       DATAIX010000702         6586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_206270, whole genome shotgun sequence.
ACCESSION   DATAIX010000702 DATAIX010000000
VERSION     DATAIX010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 6586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6586
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206270"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(352..2316)
                     /locus_tag="VHM65_10340"
     CDS             complement(352..2316)
                     /locus_tag="VHM65_10340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HEX2706147.1"
                     /translation="MPGESLFPLLEPLLERVSKPIQYVGGELNSTLKDWNCGGHGPDG
                     QELTTRWALMYPDAYEVGLPNQGVMILYEVLNERADALAERTYAVWPDMEAQLRSAGV
                     PAFTVDGHRAVGDFDLFGVSFSTELGYTNMLNALDLAGLPLHAADRGEAHPVVIAGGH
                     AAFNPEPIADFIDAAVIGDGEQAVLAITDLVGQWKAAGRPGGRHELLLRLARTGGVYV
                     PSFYDVSYRRDGRIQRVAPTPGLTGIPWRVAKHTVTDLDAWPYPKAPLVPLAETVHER
                     MSVEIFRGCTRGCRFCQAGMITRPVRERSITGIADMVERGLAATGFEEVGLLSLSSAD
                     HSEIAPLTKGLADRYEGSQTGLSLPSTRVDAFNIDLAAELARGGRRSGLTFAPEGGSE
                     RIRRVINKMVTEDDLIATVAAAYGAGWRQVKLYFMCGLPTETDEDVLQIAEVARRVIE
                     TGREVSGRRDIRCTVSIGGFIPKPHTPFQWAAQLGPEETDARLRGLRDAIRSDRRYGA
                     AIGFRYHDGKPGVVEGLLSRGDRRVGRVIEAVWRDGGRFDGWSEHFSYERWMRCAETA
                     LAREPVDVPWYTTREREHSEVLPWDHIDSGLDKDWLWDDWRDALEEVEVPDCRWTPCF
                     DCGVCPQLGTAIEIGPTGRTLLPLTVAGPG"
     gene            2394..2981
                     /locus_tag="VHM65_10345"
     CDS             2394..2981
                     /locus_tag="VHM65_10345"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2706148.1"
                     /translation="MTGDETAAPRVDGAMRMTPRTAYLPADQRARAAVDRALPIGHGQ
                     TCSQPTTVRNMLLALDVQPGQRVLDLGAGSGWTTAILARLVGPSGSVLGVERVPALAA
                     WGAAHVAAAGLPWATIVRAAPGVLGAPAQGPFDRILVSAEARTVPEALVAQLDEGGVM
                     VAPVAGELLRIRYDARTVERLGRYLFVPLVEDPGP"
     gene            complement(3105..4949)
                     /locus_tag="VHM65_10350"
     CDS             complement(3105..4949)
                     /locus_tag="VHM65_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX2706149.1"
                     /translation="MIASAHDEVVDHALIENRAQSLAHLFRSRVDATPQAEAYRYPVG
                     HEWVSVTWSQVRDLVYRYAAGLLALGVKEEDRISLASATRYEWVLADLTIACSGGATT
                     TIYPSTSPADVAYIVSDSGSTVIFAEDDAQVAKLRERRGELGDVTKVVTFDGTPDGDW
                     VISLDDLAALGDAHLAEHPTAVDDRIDALTPHHLSTIMYTSGTTGRPKGVLLTQDAFV
                     YEGAAVAAVRILDSTDLQYLWLPLSHSFGKVLLTVAMQIGLPTAVDGRIDKIVENLAV
                     VRPTFMGAAPRIFEKVHSRVTMAVAADGGIKEKLFHWAMGVGKAVDERRLAGEGPAGI
                     LALQHAIADKLVLAKVRDRFGGRVRFFISGSAALNPDVAGWFGAAGLTIIEGYGLTET
                     SAASCVNRPYPGHYRYGTVGWPLPGTEVKIAEDGEILLKGPGVMRGYHNRPEDTAEAL
                     DAEGYLHSGDIGHVDERGFVFITDRKKDLFKTSGGKYIAPSAIEAAFKGVCPYVSQML
                     VYGESRNFASALITLDPELIVGWAAQNAMEGQEYAAIVTSAAARDMVQGYVDELNQGL
                     NRWETIKSFSILDRDFTIEDGEITPSMKLRRKVVTDKYKPVLEAHYSS"
     gene            complement(5047..6009)
                     /locus_tag="VHM65_10355"
     CDS             complement(5047..6009)
                     /locus_tag="VHM65_10355"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX2706150.1"
                     /translation="MHRWTDLSQIPTDLGSTVVTLGNFDGVHRGHQAVLQRLAAEAVA
                     RSTLSVAVTFDPHPIAVLYPDRAPGAITSLDERLARIEAAGVDGVLVLEFTREFAQQT
                     PEEFVRSVFVDALRARAVVVGRDTRFGVRNSGTVDTLVALGERLGFEVVVLDDVGAGH
                     RWSSTQLRINLAEGDVAAASEILGRPHAVTGTVVHGDHRGRLLGYPTANLSQDSQGYI
                     PSDGVYAGWLVRPGLPLDAADRTLPAAISVGTNPTFNGTQRRVEAYVLDRTDLELYDE
                     YVAISFIVRLRPTLKFDSVDALIVQMRQDCADCREILGTPDPID"
     gene            complement(6047..>6586)
                     /locus_tag="VHM65_10360"
     CDS             complement(6047..>6586)
                     /locus_tag="VHM65_10360"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEX2706151.1"
                     /translation="ALLGHVARNNDQWRHATGGEALVIVHGTDHYVSPSWLPSAAQGT
                     VVPTWDYVTLHVYGSLVAHDDPEWTGTVVRRLTERHESTVAHPWTVEGAKPGYVEAMM
                     RAIVGLELKVTRVEAKAKMAQNKTPEDVAALAGALQAGGDLEGAAFLREVSLPAAVRR
                     AQTLADVAAGRHHRTPAGT"
BASE COUNT          972 a         2284 c         2356 g          974 t
ORIGIN      
        1 cacccggagg cgccaaggag gaggagcagg gcgagcgaca ggcgcatgcg cgtggtgcgg
       61 cttgcgtgtc tgcggctgtc tggcatgaca ttcctctcgg atgcggtagg tacgtgtcct
      121 tggacgccac aggccccacg aacgttccga ccgacccggg tatccgtcgg cgacaccgat
      181 gcgaggttcc cgccgcctca cctgcccggc ctcctcgggg cacccggcca acgagaccgt
      241 aacacctcac ggtgacctgc cgggcagcca agagttctgc cgggcagcca agagtgcggt
      301 gaccggaccc ggatcggggc aggtgagccg gctcagtcct tacgacggcg ctcagcccgg
      361 cccggcgacc gtcaaaggca gcagagtcct gccggtcggt ccgatctcga tcgcggtgcc
      421 cagctgcggg cacaccccgc agtcgaagca cggcgtccag cggcagtccg gcacctcgac
      481 ctcctcgagc gcgtcccgcc agtcgtccca gagccagtcc ttgtccaggc ccgagtcgat
      541 gtggtcccac ggaagcacct cggagtgctc gcgctcgcgg gtggtgtacc acggcacgtc
      601 gaccggctca cgggccagcg cggtctcggc acagcgcatc caccgctcgt aggagaagtg
      661 ctcgctccac ccgtcgaagc gcccgccgtc gcgccacacc gcctcgatga cccgcccgac
      721 ccggcgatcc ccgcgcgaga gcagaccctc gacgacccct ggcttgccgt cgtggtagcg
      781 gaaaccgatg gccgcgccat accggcgatc cgagcgtatg gcgtcacgca gcccccgcag
      841 gcgggcgtcg gtctcctccg ggcccagctg ggcggcccac tggaacgggg tgtgcggctt
      901 cgggatgaag cccccgatcg agacggtgca gcggatgtcg cgccgcccgc tcacctcgcg
      961 ccccgtctcg atgacccgcc tggcgacctc ggcgatctgc aggacgtcct cgtcggtctc
     1021 cgtcggcagg ccgcacatga agtagagctt cacctggcgc caccccgcgc cgtacgcggc
     1081 ggcgacggtc gcgatgaggt cgtcctcggt caccatcttg ttgatgaccc ggcggatgcg
     1141 ctcggacccg ccctcgggcg cgaaggtgag cccggagcgc cggccgcccc gcgccagctc
     1201 ggctgccagg tcgatgttga acgcgtcgac gcgcgtcgag ggcagcgaga gccccgtctg
     1261 ggacccctcg tagcggtcgg cgagcccctt ggtcagcggg gcgatctcgc tgtggtcggc
     1321 cgacgacagc gagaggagcc cgacctcctc gaaccccgtg gccgccagtc cgcgttcgac
     1381 catgtcggcg atgccggtga tcgagcgctc gcggaccggg cgggtgatca tcccggcctg
     1441 gcagaaacgg cagccgcgcg tgcagccgcg gaagatctcg accgacatcc gctcgtgcac
     1501 ggtctcggcg agcgggacga gcggcgcctt ggggtacggc cacgcgtcga gatcggtcac
     1561 ggtgtgcttg gcgacgcgcc agggtatgcc ggtcagcccc ggcgtggggg ccacgcgctg
     1621 gatgcgcccg tcgcggcggt agctcacgtc gtagaacgag gggacgtaga ccccgccagt
     1681 gcgcgccagg cgcagcagga gctcgtgacg gccacccggg cgacccgcgg ccttccactg
     1741 ccccacgagg tcggtgatcg cgagcacggc ctgctccccg tcgccgatca ccgccgcatc
     1801 gatgaagtcc gcgatcggct cggggttgaa cgcggcgtgc ccgcccgcga tgacgaccgg
     1861 atgcgcctcg ccacggtccg ccgcatgcag cggcaggccc gccaggtcca gggcgttgag
     1921 catgttggtg tagccgagct cggtcgagaa cgacaccccg aagaggtcga agtcaccgac
     1981 ggcgcggtgc ccgtcgacgg tgaaggccgg gacgccggcc gaacgcagct gggcctccat
     2041 gtccggccag acggcgtagg tgcgctcggc cagcgcgtca gcgcgctcgt tgaggacctc
     2101 gtagaggatc atgacgccct ggttgggcag gccgacctcg taggcgtcgg gatacatcag
     2161 cgcccaccgc gtcgtgagct cctggccgtc gggaccgtgc ccaccgcagt tccagtcctt
     2221 gagggtcgag ttgagctcgc ccccgacata ctggatcggc ttgctcaccc gctcgagcag
     2281 cggctcgagc aacgggaaca gggactcacc aggcatccgt ccatgctaac cgggatgagt
     2341 acaggtgggg ccgccgcgcc ccgcgaccgg cgggccaggg gcatagtgga ggcatgactg
     2401 gggacgagac ggcggcgccc agggtcgacg gtgccatgcg gatgacacca cgcacggcat
     2461 acctgccggc ggaccagcgg gcccgggcgg cagtcgaccg ggctctgccc atcgggcacg
     2521 gccagacctg ttcgcagccg acgacggtgc ggaacatgct gctggctctc gacgtccagc
     2581 ctggccagcg ggtgctcgac ctcggcgccg ggtcgggatg gaccaccgcg atcctcgcgc
     2641 gtctcgtcgg tcccagcggg tccgtgctcg gcgtcgagcg ggtgcctgct ctggccgcct
     2701 ggggcgccgc tcacgtcgcc gccgcgggcc tgccctgggc cacgatcgtc cgcgccgcgc
     2761 ccggggtgct cggggccccg gcgcagggac cgttcgaccg catcctcgtc tcggccgagg
     2821 cacggacggt gccggaggcg ctcgtcgcgc agctcgacga gggcggagtc atggtcgcgc
     2881 cggtcgccgg cgagctcctg cggatccggt atgacgcccg cacggtcgag cggctcggtc
     2941 gctacctgtt cgtccccctg gtcgaggacc ccggcccata gccggcccgg gccgcggcct
     3001 tcacgaccag gttcgcgaca gtgactcatc ctcgcgcagg ttagtggctc ctcagggcgc
     3061 aaacatgcgc cgggtggtgc cagtaacctg cgcgaggatg cgagctagga ggagtagtgg
     3121 gcctcgagga ccggcttgta cttgtccgtg acgaccttgc ggcgcagctt catgctcggg
     3181 gtgatctcgc cgtcctcgat ggtgaagtca cggtcgagga tcgagaagga cttgatcgtc
     3241 tcccaccggt tcaggccctg gttgagctcg tcgacgtagc cctggaccat gtctcgggca
     3301 gccgctgagg tgacgatcgc ggcatactcc tggccctcca tcgcgttctg agcggcccag
     3361 ccgacgatga gctcggggtc gagggtgatg agggccgacg cgaagttgcg gctttcgcca
     3421 tacacgagca tctggctgac gtacgggcac acccccttga acgcggcctc gatcgccgac
     3481 ggggcgatgt acttaccgcc cgaggtcttg aagaggtcct tcttgcggtc ggtgatgaag
     3541 acgaacccgc gctcgtcgac gtggccgatg tctcctgagt gcaggtagcc ctcggcgtcg
     3601 agcgcctcgg cggtgtcctc ggggcggttg tggtagcccc gcatgactcc gggacccttg
     3661 agcaggatct ccccgtcctc ggcaatcttc acctcggtgc cgggcagcgg ccagccgacg
     3721 gtgccgtacc ggtagtggcc cgggtaaggg cggttgacgc acgaggccgc gctcgtctcg
     3781 gtcaggccat agccctcgat gatcgtgagc ccggccgcgc cgaaccaccc agccacgtcc
     3841 gggttgagcg cggctgaccc ggagatgaag aaacgcactc gcccgccgaa gcggtcccgg
     3901 accttggcca ggaccagctt gtcggcgatc gcgtgctgca gcgccaggat gcccgccggg
     3961 ccctcacccg cgaggcggcg ctcgtcgacc gccttgccga cgcccatggc ccagtggaag
     4021 agcttctcct tgatcccgcc atcggcagcg accgccatcg tgacgcggga gtggaccttc
     4081 tcgaagatcc ggggggctgc tcccatgaac gtgggtcgca cgaccgcgag gttctcgacg
     4141 atcttgtcga tgcgcccgtc gaccgcggtc gggaggccga tctgcatggc gaccgtgagc
     4201 agcaccttgc cgaagctgtg cgacagcggc agccagaggt actggaggtc ggtggagtcg
     4261 aggatcctca ccgccgcgac ggccgccccc tcgtagacga acgcgtcctg agtcaggaga
     4321 acccctttgg ggcgccccgt cgtgcctgag gtgtacatga tcgtcgagag gtggtgcgga
     4381 gtgagcgcgt cgatccggtc gtcgaccgcg gtggggtgct cagcgaggtg ggcgtcaccc
     4441 agcgcggcca ggtcgtcgag gctgatgacc cagtcaccat cgggcgtccc gtcgaacgtg
     4501 acgaccttgg tcacgtcacc gagctcgccg cggcgctccc gaagcttggc cacctgggcg
     4561 tcgtcctcgg cgaagatgac cgtgctgccg gagtcgctga cgatgtaggc gacgtcggcc
     4621 ggcgacgtcg acgggtagat cgtcgtcgtc gccccgccgg agcaggcgat cgtcaggtcg
     4681 gcgagcaccc actcgtaacg cgtcgcggac gccagggaga tgcggtcttc ctccttgacg
     4741 ccgagggcaa gcagcccggc cgcgtagcgg tagacgaggt cgcggacctg ggaccaggtc
     4801 accgacaccc actcgtgccc gaccgggtag cggtaggcct cggcctgcgg agtggcatcg
     4861 acccggctgc ggaataggtg agccagggac tgcgctcggt tctcgatcag ggcatggtcg
     4921 acgacctcgt cgtgggcgct ggcgatcaag gggactcctc gaattgggga caggcgcacg
     4981 ctgaaccgcg ccgccgttgc gcgaatcttg tcagctcccg ggcccggcgt aaaggggacg
     5041 accccgtcaa tcgatcgggt caggcgtccc gaggatctcc cggcagtcgg cgcagtcctg
     5101 ccgcatctgg acgatgagcg cgtcgaccga gtcgaacttg agcgtggggc gcagccgcac
     5161 gatgaacgag atggcgacgt actcgtcata cagctcgagg tcggtgcggt cgagcacgta
     5221 ggcctccacc cggcgctgcg tgccgttgaa ggtggggttg gtaccgaccg agatcgctgc
     5281 gggcagggtc cggtccgccg cgtccagggg caggccgggc cggacgagcc acccggcata
     5341 cacgccgtcg gaggggatgt acccctgaga gtcctggctg aggttggcgg tggggtagcc
     5401 gagcagccgg ccccggtggt cgccatggac gacggttccg gtcaccgcat ggggccggcc
     5461 gaggatctca ctcgcggccg cgacgtcgcc ctcggccagg ttgatcctca gctgggtcga
     5521 ggaccagcga tggcccgcgc cgacgtcgtc caggacgacg acctcgaacc cgagccgctc
     5581 ccccagcgcc acgagcgtgt cgacggtccc tgagttgcgt actccgaagc gggtgtcccg
     5641 tccgacgacg acggctcgcg cacgcagcgc atcgacgaag accgaacgca cgaactcctc
     5701 gggtgtctgc tgggcgaact cacgggtgaa ctcgagcacg aggacgccat cgacaccggc
     5761 cgcctcgata cgtgcgaggc gctcgtcgag tgaggtgatg gcgcccggcg cgcggtccgg
     5821 gtagagcacg gcgatcgggt gcgggtcgaa ggtgacggcg accgacagcg tcgatctcgc
     5881 caccgcctcg gccgcgaggc gctggagcac ggcttgatgt ccccggtgga cgccgtcgaa
     5941 gttgcccagg gtgacgaccg tggaacccag gtcggttggg atctggctca ggtcggtcca
     6001 ccggtgcacg accgacactc taaacccgcc gccggacgcg cccggttcac gtcccagcgg
     6061 gcgtgcggtg gtgccgaccg gcggccacgt ccgccagggt ctgggcccga cgcactgcgg
     6121 cgggcagtga gacctcccgc aggaacgctg caccctcgag gtccccgccc gcctgcagag
     6181 cgccggcgag ggccgcgacg tcctccgggg tcttgttctg ggccatcttc gccttggcct
     6241 cgacccgggt gaccttgagc tcgagcccga caatcgcgcg catcatggcc tcgacatacc
     6301 ccggcttggc gccctcgacc gtccaggggt gggccaccgt gctctcgtgc cgctcggtga
     6361 gccgacggac gaccgtgccg gtccactccg ggtcgtcgtg ggcgacgagc gagccgtaga
     6421 cgtgcagggt cacgtagtcc cacgtgggca cgacggtgcc ctgcgcggct gacggcagcc
     6481 acgaagggct gacgtagtgg tcggtgccgt ggacgatgac gagcgcctca cccccggtgg
     6541 cgtgacgcca ctggtcgttg ttgcgggcga cgtggcccag cagggc
//
LOCUS       DATAIX010000703         2823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_236279, whole genome shotgun sequence.
ACCESSION   DATAIX010000703 DATAIX010000000
VERSION     DATAIX010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236279"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VHM65_10365"
     CDS             complement(<1..936)
                     /locus_tag="VHM65_10365"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX2706152.1"
                     /translation="MTRLTLGQARRSALAAQGFTDPRPAPGQVGIRHIQRVVDRVGVV
                     QIDSVNVVSRSQYLPFYSRLGAYDTALLDRARDEAPHRLVEYWAHEASLIAPATWPLL
                     TFRMRRAEHESWGGMARVAREHPALVEAVYDEVRLRGPLTSRQVEAALDHDRSGDRPD
                     WGWNWSLVKNALEHLFWAGRITSAGRTTSFERRYAALDVVAPRAGEVRRRWLDPADRR
                     CDDEAFVDLVRMAARALGVGTEQCLRDYFRLRPEQARPAIAHLVATGELVPVEVEGWR
                     RPAYLLSGTRIPRRVGARALLSPFDSLIWTRERTRA"
     gene            complement(942..1652)
                     /locus_tag="VHM65_10370"
     CDS             complement(942..1652)
                     /locus_tag="VHM65_10370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2706153.1"
                     /translation="MTIDSPGPARPDAGRTEQIRVLVADDHHLFRRGLEMVLELEGDI
                     VVVGEADDGRAAVARTEQTLPDIVLMDVKMPKLNGIEACRAIKELVPSTRILMLTMSD
                     DEQDLFEAIRAGANGYLLKDVPGEQIAEGLRAVYQGQSLISPSMAAKLLTEFATLSRR
                     QERAVTAPVPRLTDREREVLGLVARGLSNREIGKALFISENTVKNHVRNILEKLQLHS
                     RMEAALYAVRERLFDAPE"
     gene            complement(1698..2384)
                     /gene="raiA"
                     /locus_tag="VHM65_10375"
     CDS             complement(1698..2384)
                     /gene="raiA"
                     /locus_tag="VHM65_10375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX2706154.1"
                     /translation="MFRVARPARHQPYRSLQEDIVEIVVTGRHVQLSDRFRQHIEDKL
                     AKIEQLAPEVHRVDVVVTHEPTARKSERVEVTCHAKGPVIRAEAAMDDRFAAFESAFD
                     KTTERLRRAQDRRRVRRGRRTPESVGSATARIVPPAESAAPTADQMDLDGQGLGTLAD
                     SPIEVREKVHTSRPMTLEEALTRMELVGHDFFLYHDRETGKPSVVYRRRGWAYGVIHL
                     DLHGADDHAG"
     gene            complement(2481..>2823)
                     /locus_tag="VHM65_10380"
     CDS             complement(2481..>2823)
                     /locus_tag="VHM65_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX2706155.1"
                     /translation="EQLARRALEVSATSYLVPEAIVVRAVEAVRRVADQAALGHDARA
                     VNVAFAYDVRPGLGRAVRGRVCVLVDDVVTTGATLAEAARALRRAGAVVPVAATVAAT
                     ALRREVPHRWT"
BASE COUNT          387 a          984 c         1044 g          408 t
ORIGIN      
        1 cgccctggtc cgctcgcgcg tccagatgag cgagtcgaac gggctgagca aggcccgcgc
       61 gccgacccgc ctcgggatcc tggtccccga gagcaggtat gccgggcggc gccagccctc
      121 gacctcgacc gggaccagct cgccggttgc gacgaggtgc gcgatggcgg ggcgcgcctg
      181 ctccggccgg agccggaagt agtcgcgcag gcattgctcg gtcccgacgc cgagagcccg
      241 agccgccatc cggacgaggt cgacgaacgc ctcgtcgtcg cagcggcggt cagccgggtc
      301 gagccagcgc cggcgaacct cgccggcgcg tggcgccacg acatcgagtg ccgcatatcg
      361 gcgctcgaag gacgtcgtgc gcccggcgct cgtgatgcgc ccggcccaga agaggtgctc
      421 gagggcgttc ttgaccaggg accagttcca gccccagtcg gggcggtcgc cgctgcggtc
      481 gtggtcgagc gcggcctcga cctgacggga cgtgagcggg ccgcggaggc gcacctcgtc
      541 atacaccgcc tcgacgagtg cgggatgctc ccgcgcgacc cgggccatcc caccccagga
      601 ctcgtgctcg gcgcggcgca tccggaaggt gagcaggggc caggtcgcgg gcgcgatgag
      661 gctggcttcg tgggcccagt actcgacgag gcggtggggt gcctcgtcgc gggcccggtc
      721 gagcagagcg gtgtcgtagg ccccgagccg ggagtagaac ggcaggtact ggctgcggct
      781 cacgacgttg acgctgtcga tctggacgac gccgacccgg tcgacgaccc gctggatgtg
      841 ccggatcccc acctggccag gcgccgggcg ggggtcggtg aagccctggg ccgccagcgc
      901 ggaacgtcga gcctgcccga gggtcagccg ggtcaccccg ctcactcggg ggcgtcgaac
      961 aggcgctctc gcacggcgta gagggcggcc tccatgcgcg agtgcagctg gagcttctcc
     1021 aggatgttgc ggacgtggtt cttgacggtg ttctcggaga tgaacagcgc cttgccgatc
     1081 tcgcggttgc tcagcccgcg ggcgacgagc ccgagcacct cgcgctcacg gtcggtcagc
     1141 cgggggaccg gagctgtcac cgctcgctcc tgacgccggc tcagggtcgc gaactcggtg
     1201 agcagcttgg ccgccatcga cggggagatg agcgactgcc cctggtagac cgcccgcagc
     1261 ccctcggcaa tctgctcgcc ggggacgtcc ttgagcaggt agccgttggc tccggcgcgg
     1321 atcgcctcga agaggtcctg ctcgtcgtcg gacatcgtga gcatgaggat gcgggtcgac
     1381 gggaccagct ctttgatggc ccggcacgcc tcgatgccgt tgagcttggg catcttgacg
     1441 tccatgagca cgatgtcggg aagggtctgc tcggtccggg ccacggcggc tcgcccgtcg
     1501 tcggcctccc cgacgacgac gatgtcgcct tcgagctcga ggaccatctc cagtccgcgc
     1561 cggaacaggt ggtggtcgtc ggcgacgagg acgcggatct gctccgtccg gccagcatcg
     1621 ggcctcgccg ggccgggcga gtcgatcgtc accacgtcat cctcccacgg acgggtccgg
     1681 gcccttcctg acggcgctca ccccgcgtgg tcgtccgcgc cgtgaaggtc gagatggatg
     1741 acgccgtagg cccagccgcg gcggcggtag acgacgctcg gcttgccggt ctcgcggtcg
     1801 tggtagagga agaagtcgtg gccgacgagc tccatccggg tcagggcctc ctcgagggtc
     1861 atcggccggc tggtgtggac cttctcgcgg acctcgatcg gcgagtcggc cagcgtgccg
     1921 agcccctgcc cgtcgagatc catctggtcg gccgtcggcg cagccgactc ggcagggggc
     1981 acgatccgag ccgtggccga gcctaccgac tcgggcgttc gccggccccg gcggacccgg
     2041 cggcggtcct gcgcgcggcg caggcgctcg gtcgtcttgt cgaaggcgga ctcgaaagcg
     2101 gcgaaccggt catccatggc cgcttcggcg cggatcaccg ggcccttggc gtgacaggtc
     2161 acctcgacgc gctcggactt gcgggcagtg ggctcgtggg tgacgacgac atcgacacgg
     2221 tgcacctcgg gcgcgagctg ctcgatcttg gcgagcttgt cctcgatgtg ctggcggaag
     2281 cggtccgaga gctggacgtg acggccggtg acgacgatct ccacgatgtc ctcctgaagg
     2341 gaacggtagg gctggtgacg cgcgggccgg gcgaccctga acacccgcgg catcaccccg
     2401 actcgtgcgt cacgtgctcg ggtccatacc caaacgctat ctcgccgcgg tgatgccgtc
     2461 atgccgtggc cgccgtagcg tcatgtccac cggtgcggca cctcacgacg caacgcggtg
     2521 gcggcgaccg tcgccgcgac cgggacgacg gcaccggcgc gccgcagggc ccgcgcggct
     2581 tcggcgagcg tcgccccggt ggtcacgacg tcgtcgacga gcacgcagac gcgcccgcgg
     2641 accgcgcgtc ccagcccggg gcggacgtca taggcgaacg ccacgttgac ggcccgtgcg
     2701 tcgtgaccca gggctgcctg gtccgcgacg cggcgcaccg cttcgacagc gcggaccacg
     2761 atggcctccg ggacgaggta tgacgtggcg ctcacctcga gcgcccgacg tgccagctgc
     2821 tcc
//
LOCUS       DATAIX010000704         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_206485, whole genome shotgun sequence.
ACCESSION   DATAIX010000704 DATAIX010000000
VERSION     DATAIX010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206485"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VHM65_10385"
     CDS             complement(<1..191)
                     /locus_tag="VHM65_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706156.1"
                     /translation="MVQPPAQVDGSVPLSCLICYDLDYSDYVRAVARHGGVLVAPSND
                     WREYADLHDGLALWGPVMTG"
     gene            complement(200..535)
                     /locus_tag="VHM65_10390"
     CDS             complement(200..535)
                     /locus_tag="VHM65_10390"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2706157.1"
                     /translation="MAHHLQENPDLCFVAADDSDQVVGFALGASTYEILADTGHLEWV
                     AVAPSRRGEGIARRLLDTAVDAFRRVGRRQVVADASSDNAASRALFARAGWHEGISVT
                     FFVRDLDEG"
     gene            798..1187
                     /locus_tag="VHM65_10395"
     CDS             798..1187
                     /locus_tag="VHM65_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706158.1"
                     /translation="MLTRLLELLRREPILLYGIGSVLVLAVLLVGVGAVADQVPLIVA
                     AAVLLLLAALAAGTYVLVRKPTAPPRVDVSGRNIKAARDSAIVREEGAAQPSGVRVTA
                     TGDVVASDSSAIVDVGSRRRPARRRIR"
     gene            1200..2003
                     /locus_tag="VHM65_10400"
     CDS             1200..2003
                     /locus_tag="VHM65_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706159.1"
                     /translation="MTGVDEGAFVGREEIIAEVLRWVPDEQGRVPARGEIGPGSPATR
                     QHAHGTWGIGKTTLLDELERQLRATSEALILRVDAGEWVPTAEPGPESGLPLRSLHAN
                     FLQFVGMLEHVCLSSKDLHREALLVREARAHVNMQRDAPKVETAVHAGGNISAQGESS
                     IISVYLDEQRDEELCTKILEIVSPNGTNVVLQSMRASVRGHRSPMMRAGVTTMIITVP
                     EARTIQRPRPAQTCARTMTTPATTEPRAVAIEAPPKAVLTFSALVEVTV"
BASE COUNT          381 a          745 c          760 g          294 t
ORIGIN      
        1 ccagtcatca cagggcccca gagggcgagc ccatcgtgga ggtcggcgta ctcgcgccag
       61 tcgttggacg gcgccaccag gaccccgccg tgccgggcga cggcccgcac gtagtcggag
      121 tagtcgaggt cgtaacagat gaggcacgag aggggaaccg agccgtcgac ctgcgccggc
      181 ggctggacca cgcgacggct cagccctcgt cgaggtcccg gacgaagaag gtcaccgaga
      241 tgccttcgtg ccacccagct cgggcaaaca gcgcccggga ggcggcgttg tccgatgagg
      301 cgtcggcgac aacctgccgc cgcccaacac gccggaaggc gtcgaccgct gtgtcgagca
      361 ggcggcgcgc gatgccctcc ccgcgccgcg acggcgccac ggcgacccac tccaggtggc
      421 cggtgtccgc gaggatctca tacgtcgacg cgccgagcgc gaagccgacc acctggtcgc
      481 tgtcgtccgc agcgacgaag cacaggtcgg gattctcctg cagatggtgc gccacctcga
      541 cgagcgatca gtagttgtaa ggcttgtcgg tgacgacgta gcagcgcgag ccgagctcga
      601 agacggcggg gacgtcggcg acccgcatcg gccgcacgac cgtcaccata ggcgcagtca
      661 accaccgccg cggcgacgcg caaacgagta cgacagtctc cttgtcgact tgtcgacgcc
      721 ccggacagcg atcagcatga gagccgccaa ctgagaggtg gcccatgacg accggaacca
      781 acggcgtgcc cgacagggtg ctcacccggc tgctggagct gctgcgccgg gagccgatcc
      841 tgctgtatgg gatcggctcg gtgctcgtgc tcgccgtgct gctcgtcggc gtgggcgccg
      901 tcgccgacca ggtaccgctg atcgtcgccg ccgccgtgct gctcctgctg gccgcgctcg
      961 ccgccggcac ctacgtcctc gtgcgcaaac ccactgctcc accgcgcgtg gacgtcagcg
     1021 gccggaacat caaggctgca cgggactcgg cgatcgtgcg cgaggaaggt gccgcgcagc
     1081 cgagcggtgt ccgggtgacc gccaccggcg acgtggtagc cagcgacagt tcggcgatcg
     1141 tcgacgtcgg gtcccggcgg cggccggcgc ggcgccgtat tcgctagaag ccccctgaga
     1201 tgaccggcgt cgacgagggg gcgttcgtcg gtcgcgagga gatcattgcc gaggtcctga
     1261 ggtgggtgcc cgacgagcag ggccgggtgc ctgcccgcgg cgaaatcggg cctggctcac
     1321 cggccacgcg gcagcacgca cacggcacgt ggggcatcgg caagacaacg ctgctcgacg
     1381 agctcgagcg acagttgcgc gccacttccg aggcgctgat cctccgcgtg gatgctggcg
     1441 agtgggtgcc aacagcggag ccgggaccgg agagtgggct gccgctgcgc tcgctgcacg
     1501 ccaacttcct acagttcgtg ggcatgctcg agcacgtctg cctgtcgtcg aaggacctcc
     1561 accgcgaggc gctgctcgtg cgggaggcgc gcgcccacgt caacatgcag cgtgacgcgc
     1621 ccaaggtcga gaccgcggtg catgccggcg gcaacatcag cgcgcagggt gagagctcga
     1681 tcatcagcgt ctaccttgac gagcagcggg acgaggagct gtgcacaaag atcctcgaga
     1741 tcgtcagccc taacggcacg aacgtagtgc tccagagcat gagagcgagt gtccggggcc
     1801 acaggtctcc catgatgaga gccggtgtga cgacgatgat catcacggtt ccagaggcga
     1861 ggaccatcca gcgaccgagg cccgcccaga cgtgtgcgcg gacgatgacg acaccagcga
     1921 cgactgagcc aagggcggtt gccatcgagg cccccccgaa ggccgtgctg acgttctccg
     1981 cgctcgtcga ggttaccgtc tgatcaccga tcgcgccgct ggccaactcg cagagggcca
     2041 ggacggcgaa tcccacgacc gcgacccaca gcgcgatcgc cgcggtacga cttcgccgat
     2101 gagggttagc taggacgacc ccgacgaaac ctgccgcggt caaggcgtgc gccagcgcca
     2161 agacgatgct gcttccccat
//
LOCUS       DATAIX010000705         1755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_206500, whole genome shotgun sequence.
ACCESSION   DATAIX010000705 DATAIX010000000
VERSION     DATAIX010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206500"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VHM65_10405"
     CDS             <1..950
                     /locus_tag="VHM65_10405"
                     /inference="COORDINATES: protein motif:HMM:NF028228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2706160.1"
                     /translation="RAGGAASSSAASLLFDTPPIATDAVRWESLPRLRRDSSDPYAGW
                     LQTVTVADAEKRIAQLAQAPGRSAEIEIETGYAGLEAGQIAEVVGAVNRMLTADPWEW
                     RAVWMSGLAALARDDSPSAQSAFNAVYGQVPGELAPKLGLAYACERSNEVDIAESLYT
                     TCARTDAAYVPTAAFGLARLRAGRQDIDAAASALDLVPESSRAYPAAQQEAAHLLATH
                     SRDIADLSRAMTIVDRAAFAPRQRAQLAVTIYRNGLAAVAVEKAPSGVVIGGQQATAT
                     GLRRGLEQSLRDLAKASDNASERVALVDEANAVRPWSLR"
     gene            956..>1755
                     /locus_tag="VHM65_10410"
     CDS             956..>1755
                     /locus_tag="VHM65_10410"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX2706161.1"
                     /translation="MGSACPACGATTAPDSAFCEACGADLAGGAARLAPPGETTVLAA
                     ATPVTSHAGEESPLDVGWTGVVAGATVTRVPDAVNPGPRCERCREGQVVDGYCDVCGS
                     PPRDPRDHFTEQPAPWIAAVCDIGIRHSRNEDGLASMAQDTGQRRAAVVVCDGVSNTT
                     DSHIASLAAARAARDVLAVAPAQGVGTRESRVAAIRRMLGQAVLAADRAVERAVPEGT
                     DNPPSSTFVAAVVAGSLAVVGNVGDSRAYWVPDDLTADARQLSTDDSWA"
BASE COUNT          236 a          663 c          641 g          215 t
ORIGIN      
        1 accgagcggg cggcgcggcc agctcctcgg cggcgtcctt gctgttcgac accccaccga
       61 tcgcgaccga cgcggtgcgg tgggagagcc tcccgcggct gcgccgcgac tccagcgacc
      121 cctatgccgg gtggctccag accgtgacgg tcgccgatgc cgagaaacgc atcgcccagc
      181 tcgcccaggc gcccggacgg tcggcggaga tcgagatcga gaccgggtat gccgggctgg
      241 aggccgggca gatcgccgag gtcgtcggcg ccgtcaaccg gatgctcacg gccgacccgt
      301 gggagtggcg cgccgtgtgg atgtccggtc tggcggcgct cgcccgcgac gactcaccga
      361 gcgcacagag cgcgttcaac gcggtctacg ggcaggtccc gggtgagctc gcccccaagc
      421 tcgggctggc ctacgcctgc gagcgcagca acgaggtcga cattgccgag tcgctgtaca
      481 ccacctgcgc gcgcaccgac gccgcctacg tgccgaccgc cgccttcggg ctggcgcggc
      541 tccgggccgg acgccaggac atcgacgccg cggcgagcgc gctcgacctg gtcccggaga
      601 gctcgcgtgc ctaccccgct gcccagcagg aggccgcgca cctgctcgcg acccacagcc
      661 gtgacatcgc cgacctgtcc cgggcgatga cgatcgtcga ccgcgccgcg ttcgcgcccc
      721 gccagcgggc ccagctcgcg gtgacgatct accgcaacgg cctcgccgcc gtcgccgtcg
      781 agaaggcccc cagcggtgtc gtgatcggcg gtcagcaggc cacggcgacc ggtttgcgcc
      841 gcggcctcga acagtcgctg cgagacctgg ccaaggcgag cgacaacgcc tccgaacggg
      901 tcgcgctcgt cgacgaggcc aacgcggtgc gcccctggag cctgcggtga gcggcgtggg
      961 ttccgcgtgc ccggcgtgcg gtgccacgac ggctcccgac tcggcgttct gtgaagcctg
     1021 cggagccgac ctcgccggag gggcggcacg gctcgcaccg ccgggcgaga cgacggtcct
     1081 cgccgcggca accccggtca ccagccacgc cggtgaggag tcgccgctcg acgtcggctg
     1141 gaccggtgtc gttgccggcg cgaccgtcac ccgggtcccc gatgcggtca acccgggacc
     1201 gcggtgcgag cggtgccggg aaggtcaggt cgtcgacggc tactgcgacg tgtgtggcag
     1261 cccgccacgc gaccctcggg accacttcac ggagcagccg gcgccgtgga tcgcggcggt
     1321 ctgcgacatc gggatccgtc acagccgcaa cgaggacgga ctcgcgtcga tggcccagga
     1381 caccggccag cgccgcgccg cggtcgtcgt ctgtgacggg gtcagcaaca cgaccgactc
     1441 gcacatcgcg agcctggccg ccgcccgcgc ggcccgggac gtcctcgccg ttgccccggc
     1501 gcagggggtg gggactcggg aaagccgcgt ggccgccatc cggcgcatgc tcggtcaggc
     1561 cgtcctcgcc gcggaccggg cggtcgaacg ggctgttccc gaggggaccg acaaccctcc
     1621 gtcgagcacg ttcgtcgcgg cggtcgtcgc cgggtcgctc gccgtggtgg gcaacgtcgg
     1681 ggacagccgg gcctactggg ttccggacga cctcaccgcc gacgcccgcc agctcagcac
     1741 cgacgactcg tgggc
//
LOCUS       DATAIX010000706         1742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_236499, whole genome shotgun sequence.
ACCESSION   DATAIX010000706 DATAIX010000000
VERSION     DATAIX010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236499"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1605)
                     /gene="purF"
                     /locus_tag="VHM65_10415"
     CDS             complement(31..1605)
                     /gene="purF"
                     /locus_tag="VHM65_10415"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX2706162.1"
                     /translation="MARGDGRLTHDLLPGEKSPQDACGVFGVWAPGEDVAKLTYFGLY
                     ALQHRGQESAGIATGDGNKVLVYKDMGLVSQVFDEAALVALKGHLAIGHCRYSTTGGS
                     TWENAQPTLGGGDHTTVALAHNGNLTNSAHLREVLTESLGPLGSHPGELGRGNTTDTA
                     LVTTLLVSNADGTLEDRALEVLPMLEGAFCFVFMDEQTLYAARDRHGIRPLALGRLDR
                     GWVVASETAALQTVGAAVIREIEPGELIVVDEHGLRSHRFATATPTGCAFEYVYLARP
                     DAVISGRVVHEARVEMGRRLAREHPVEADLVIGVPESGVPAATGYAQESGIPYGQGFV
                     KSAYVGRTFIQPSQTLRQLGIRLKLNPLGHTIKGRRLVVVDDSIVRGNTQRAQVRMLR
                     EAGAAEIHVRISSPPVGWPCFYGIDFATRAELIATGLGVEEIRASIDADSLGYISEEG
                     MVAATGQPSSALCTACFNGHYPIELPATEHLGKHLFETLPLDLDLDLGPPRGPRDVEG
                     VSVGVAGGAANALLHP"
BASE COUNT          252 a          600 c          653 g          237 t
ORIGIN      
        1 cgggagaggc tgggtgggtc atcggcgtcc tcaggggtgc agcagcgcgt tcgcggcgcc
       61 accggcgacc ccgacggaga ccccctccac gtcgcggggg ccgcgaggcg ggccgaggtc
      121 gaggtcgagg tcgagcggca gggtctcgaa gaggtgcttg ccgaggtgct cggtggccgg
      181 cagctcgatc gggtagtgcc cgttgaaaca cgccgtgcac aacgccgacg acggctggcc
      241 ggtggccgcc accatgccct cctcgctgat gtagccgaga ctgtccgcgt cgatgctggc
      301 ccggatctcc tcgacgccga ggccggtcgc gatgagctcg gcgcgggtgg cgaagtcgat
      361 gccgtagaaa cacggccacc ccaccggggg ggaggagatc cgcacgtgga tctcggcggc
      421 accggcctcg cgcagcatcc ggacctgggc ccgctgggtg ttgccgcgca cgatcgagtc
      481 gtccacgacg acgaggcgcc tgcccttgat cgtgtggccg agcgggttga gcttgagccg
      541 gatgccgagc tggcgcaggg tctgcgaggg ctggatgaag gtgcgcccga cataggcgct
      601 cttgacgaac ccctgcccgt aggggatgcc cgactcctgc gcgtagcccg tcgcggcagg
      661 cacgcccgac tcgggcaccc cgatgacgag gtcggcctcg accggatgct cgcgcgccag
      721 ccgccggccc atctcgaccc gggcctcgtg gacgacgcgc ccgctgatga cggcgtcggg
      781 ccgggcgagg tagacgtact cgaaggcgca gcccgtcggc gtggccgtcg cgaaccggtg
      841 cgagcgcagc ccgtgctcgt cgacgacgat gagctcacct ggctcgatct cgcggatcac
      901 cgcggcgccg acggtctgca gcgcggcggt ctcggacgcg acgacccagc cccggtcgag
      961 ccgccccagc gccagggggc ggatcccgtg gcggtcgcgc gcggcataca acgtctgctc
     1021 gtccatgaag acgaagcaga acgcaccctc gagcatgggc aggacctcga gggcgcggtc
     1081 ctcgagcgtg ccgtccgcgt tgctgacgag cagggtggtg accagggccg tgtcggtcgt
     1141 gttgccgcgg ccgagctcac cggggtgcga gccgagcggg ccgagcgact cggtgaggac
     1201 ctcgcgcagg tgggcggagt tggtgaggtt gccgttgtgg gcgagggcga cggtcgtgtg
     1261 gtcgccgccg ccgagggtcg gctgggcgtt ctcccaggtc gagccgccgg tcgtcgagta
     1321 gcggcagtgc ccgatcgcga ggtggccctt gagggcgacc agcgcggcct cgtcgaagac
     1381 ctgcgagacc aagcccatgt ccttgtagac gaggaccttg ttgccatcgc cggtcgcgat
     1441 gcccgccgac tcctgcccgc ggtgctgcag ggcgtagagc ccgaagtagg tgagcttggc
     1501 cacgtcctcg cccggggccc acactccgaa gacgccacac gcgtcctggg gtgacttctc
     1561 tcccgggagg aggtcgtgcg tcagccgacc atcaccacgc gccacggcgc aagtctccca
     1621 cagccccgtg cagaccagga ccggctatcc cccacgccgg tcgagcagga tcgcgacgac
     1681 acccccgagc agggcgccga gcccggcgcc gatgaccgcg aagtacccga cggacgtgct
     1741 cg
//
LOCUS       DATAIX010000707         4566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_259010, whole genome shotgun sequence.
ACCESSION   DATAIX010000707 DATAIX010000000
VERSION     DATAIX010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259010"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VHM65_10420"
     CDS             <1..287
                     /locus_tag="VHM65_10420"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HEX2706163.1"
                     /translation="ATRGLARGALAAELRGRGVDPGIAHDALAVVGDADERARAEALV
                     TKRLRAMHGLAPDVQARRLAGMLARKGYPTAIALAVVRDAIANAPEHRRD"
     gene            complement(302..946)
                     /locus_tag="VHM65_10425"
     CDS             complement(302..946)
                     /locus_tag="VHM65_10425"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEX2706164.1"
                     /translation="MSSRELILNRIRTANRSGDLAGPDDGGRSWAYGQPTAMEDVLTR
                     FVDRVQDYQATVIRCSEGRVAEHIGEALSEVHVTSVVLPSGAPPAWRDAVRARGIEVR
                     DDTPLSHAELDDTGAVVTGSRVSIAETGTIVLDHGPDQGRRALTLVPDVHVCVVRSEQ
                     VVSDVPEAVQVLAATIATGHPLTWISGGSATSDIELDRVEGVHGPRTLIVLLVG"
     gene            complement(943..2427)
                     /locus_tag="VHM65_10430"
     CDS             complement(943..2427)
                     /locus_tag="VHM65_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HEX2706165.1"
                     /translation="MTTSTRRTPAPPEGTYLGMPPFPEAAKAALENTQLRKNLRHATG
                     TIRAKRGRVVAEVAGWEDLRVAGAAIKDRTLRNLDTYLVHFEKAMTAAGGHVHWATDA
                     DEANRIIVDLVRAQGADEVVKVKSMATQEIDMNEALEEAGIAAWETDLAELIVQLGHD
                     RPSHILVPAIHRNRSEVREIFLREMGLYGAPAPDGMSDEPAVLAGAARLHLREKFLRA
                     DVAISGANFAIAQTGTLVVVESEGNGRMCLTLPQTLISVVGIEKILPTFADLEVFLQL
                     LPRSSTGERMNPYTTMWTGVSPGDGPQNVHVVLIDNGRTKVLADSVGRQALRCIRCSA
                     CLNICPVYERVGGHAYGSVYPGPIGAVLTPQLRGTASAIDKSLPYASSLCGACFEVCP
                     VRIELPELLVHMRGKVVETKRKGVPHTEALAMRAGSWMLGDHRRFEAAQKAATTGRKL
                     MPGTTHLGALPWPLARWANARDVPMPPRETFRSWWKRERGGESS"
     gene            complement(2424..3233)
                     /locus_tag="VHM65_10435"
     CDS             complement(2424..3233)
                     /locus_tag="VHM65_10435"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX2706166.1"
                     /translation="MTTADTVGTGTLVSDWREKPGSGMTVALCGTCINDTMFPQTLKA
                     VVTVLERLGCAVEYPMAQTCCGQMFTNTGYFAEAMPTVRTYLEAFEGYDYIVGPSGSC
                     VGSVREQHPMLAERSGDARLVRRVSEIVTHTYELSELLVDVLGVTDVAAYFPHRVTYH
                     STCHSIRVARVGDRPYRLLRAVQGIDLVPLVDSDQCCGFGGTFSVKNPDVSVAMGSDK
                     ARHVMETGAQYVVAGDNSCLMHVGGLLHRMRSGVAPIHLAEILANTAEEQS"
     gene            3371..>4566
                     /gene="miaB"
                     /locus_tag="VHM65_10440"
     CDS             3371..>4566
                     /gene="miaB"
                     /locus_tag="VHM65_10440"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018551536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX2706167.1"
                     /translation="MRTYEVRTHGCQMNVHDSERLAGLLETAGYVDHASLPAHERPDT
                     ADVVVFNTCAVRENADNKLYGNLGQLRPAKTKNPDLQIAVGGCLAQKDRAAIVDRAPW
                     VDVVFGTHNIGSLPALLDRARHNKLAQVEILESLETFPSTLPTRRDSAYAAWVSISVG
                     CNNTCTFCIVPSLRGKEQDRRPGEILAEVDALVAQGVIEVTLLAQNVNTYGVEFGDRL
                     AFGTLLRSCGDVVGLERVRFTSPHPAAFTSDVIEAMAATPTVMPSLHMPLQSGSDRVL
                     AAMRRSYRSERFLAIIDEVRAQIPDAAITTDLIVGFPGETEADFEATLDVVRASRFSS
                     AFTFQYSVRPGTPAATMGDQVPKSVVQERFDRLIAVQEQVSWAENRRQEGRGVEVLVA
                     TGEGRK"
BASE COUNT          680 a         1616 c         1584 g          686 t
ORIGIN      
        1 aggcgacccg tggcctggcc cgtggcgccc tggccgccga gctgcgcggc aggggcgtcg
       61 acccaggtat cgcccacgac gcgctggcgg tggtcggcga tgctgacgag cgggcgcgag
      121 ccgaggccct cgtcaccaaa cgccttcgag ccatgcacgg gctggcgccc gacgtgcagg
      181 ctcgacggtt ggcggggatg ctggcccgca agggctaccc gaccgcgatc gccctggccg
      241 tggtccgtga cgcgatcgcc aacgcccccg agcatcggcg cgactgactg acctctgact
      301 cctacccgac gagcaggacg atcagcgtgc ggggtccgtg cacgccttcg acgcggtcga
      361 gctcgatatc gctggtcgcg ctccctccac tgatccaggt gagggggtgg ccagtggcga
      421 tcgtcgctgc caggacctgc acggcttccg gcacgtcgga gacgacctgc tcggaacgga
      481 ccacgcagac gtgcacatcc ggcacgagag tgagcgcccg gcggccctgg tcgggtccgt
      541 ggtcgaggac gatcgtcccg gtctcggcga tcgacacgcg ggagcccgtg actacggcgc
      601 cggtgtcgtc gagctcggcg tggctcagcg gcgtgtcatc ccggacctcg atcccgcgcg
      661 ccctgaccgc atcccgccat gctgggggag cgccactcgg gaggaccact gaggtgacgt
      721 gcacctcgct cagtgcctcg ccgatgtgct cggccacccg gccctccgag caccggatca
      781 ccgtggcctg gtagtcctgg acccggtcga cgaagcgggt cagcacgtcc tccatcgcgg
      841 tcggctgccc gtaagcccag gagcgcccac cgtcatccgg cccagccagg tccccgctgc
      901 ggttggcggt gcggatccgg ttgaggatca gctctcggct gctcatgacg actcccctcc
      961 ccgctcgcgc ttccaccagg accggaacgt ctccctcggt ggcatcggca catccctggc
     1021 gttggcccat cgggccagcg gccacggcag tgccccgagg tgagtcgtgc ctggcatcag
     1081 cttgcgcccg gtggtcgcgg ccttctgcgc ggcctcgaaa cgccgatggt cgccgagcat
     1141 ccaggagccc gccctcatcg cgagcgcctc ggtgtggggg actcccttgc gcttggtctc
     1201 gaccaccttg cctcgcatgt ggacgagcag ctcggggagc tcgatccgca ccgggcagac
     1261 ctcgaagcag gcgccgcaca gggacgacgc atacggcagc gatttgtcga ttgcactcgc
     1321 cgtgccccgc agctgtgggg tgagcaccgc gccgatgggc ccgggataca ccgacccgta
     1381 ggcgtggccg ccgacccgct cgtagaccgg gcagatgttg aggcaggccg agcaccggat
     1441 gcagcgcagc gcctggcggc cgaccgagtc cgcgaggacc ttggtccggc cgttgtcgat
     1501 gagcacgacg tggacgttct gcggaccgtc accgggcgac accccggtcc acatcgtcgt
     1561 gtaggggttc atccgctcac cggtgctgct gcgcggaagg agctggagga agacctcgag
     1621 gtcggcgaag gtcggcagga tcttctcgat cccgacgacc gagatcagcg tctgcggcag
     1681 ggtgaggcac atccgtccgt tgccttcgga ctcgacgacg acgagcgtcc cggtctgcgc
     1741 gatggcgaag ttcgccccgg agatcgccac gtcggctcga aggaacttct cccgcaggtg
     1801 aagccgcgca gccccggcga gcacggccgg ctcgtcgctc atcccgtccg gcgccggcgc
     1861 gccatacagg cccatctcgc gcaggaagat ctcgcggacc tcggagcggt tgcggtggat
     1921 cgccgggacg aggatgtggc tcggccggtc gtggcccagc tggacgatga gctcggcgag
     1981 gtcggtctcc caggcggcga tacccgcctc ctcgagcgcc tcgttcatgt cgatctcctg
     2041 ggtggccatc gacttgacct tgacgacctc gtcggcaccc tgtgcccgga cgaggtcgac
     2101 gatgatgcgg ttggcctcgt cggcgtcggt cgcccagtgg acgtgcccac cggcggcagt
     2161 catcgccttc tcgaagtgga cgaggtacgt gtcgaggttg cgcagggtgc ggtccttgat
     2221 cgccgcaccg gccacgcgca ggtcctccca gccggcgacc tccgcgacga cccggccgcg
     2281 cttggcccgg atcgtccccg tcgcatggcg caggttcttg cgcagctggg tgttctccag
     2341 ggccgccttc gccgcctccg ggaagggggg catgccgagg taggtgcctt ccggcggtgc
     2401 aggggtgcgg cgggtcgacg tcgtcatgac tgctcctccg cagtgttggc gaggatctcg
     2461 gcgaggtgga tcggtgcgac gccactgcgc atccggtgga gcagccctcc gacgtgcatg
     2521 aggcaggagt tgtcccccgc gacgacgtac tgcgcgccgg tctccatgac gtggcgggcc
     2581 ttgtccgacc ccatcgcgac cgagacgtcc gggttcttga ccgagaaggt cccgccgaag
     2641 ccgcagcact ggtcggagtc gaccagcggc acgaggtcga tgccctggac cgcgcgaagc
     2701 agccggtagg gacggtcgcc gacccgggcg acccggatcg agtggcaggt cgagtggtag
     2761 gtcacccgat gcgggaagta ggccgcgacg tcggtgaccc ccaggacgtc gacgagcagc
     2821 tcggacagct cgtaggtgtg cgtgacgatc tcgctgacgc gccgcacgag cctggcgtct
     2881 ccggaccgct cggccagcat cgggtgctgc tcccggaccg acccgacgca cgagccggac
     2941 gggccgacga tgtagtcata gccctcgaag gcctcgaggt aggtccgcac ggtgggcatc
     3001 gcctcggcga agtaccccgt gttggtgaac atctgaccgc agcaggtctg cgccatcggg
     3061 tactccacag cgcagcccag ccgctcgagc acggtcacca cggccttgag cgtctgcggg
     3121 aacatcgtgt cgttgatgca cgtgccgcag agggccaccg tcatgcccga cccgggcttc
     3181 tcgcgccagt cggacacgag ggtgcccgta ccgaccgtgt ccgccgtcgt catggccgtc
     3241 tcccgtcccg tcgatgcttc ggcaacgggg ccaacgctac tcggagtacg ccacaccgcc
     3301 cgtgacgatg tgctgcgccg ggagagggcg gtgcggggcg gcggcaggac gacgtaccct
     3361 tgaacccgcc atgaggacct acgaggtacg cacccacggg tgccagatga acgtccatga
     3421 cagcgagcgc ctcgccgggc tgctggagac cgccgggtat gtcgaccacg cgtcactccc
     3481 ggcgcacgag cgccccgaca ccgccgacgt cgtcgtgttc aacacgtgcg ccgtccgcga
     3541 gaacgccgac aacaagctct acggcaacct cggccagctg cggcccgcca agacgaagaa
     3601 ccccgacctg cagattgccg tcggcggctg cctcgcccag aaggaccgcg ccgcgatcgt
     3661 cgaccgggcg ccctgggtcg atgtcgtctt cgggacccac aacatcggct cgctgccggc
     3721 gctgctcgac cgggcgcgcc acaacaagct ggcgcaggtc gagatcctcg agagcctcga
     3781 gacatttccc tcgaccctgc ccacgcggcg cgactcggca tacgccgcct gggtctcgat
     3841 ctcggtcggc tgcaacaaca cctgcacgtt ctgcatcgtc ccgagcctgc ggggcaagga
     3901 gcaggaccgc cggccggggg agatcctcgc cgaggtcgac gcgctcgtcg cccaaggcgt
     3961 gatcgaggtc acgctgctgg cgcagaacgt caacacgtat ggcgtcgagt tcggcgaccg
     4021 gctcgcgttc ggcacgctgc tgcgttcctg cggcgacgtc gtgggccttg agcgggtgcg
     4081 gttcacgagc ccgcacccgg cggccttcac gagcgacgtc atcgaggcga tggcagccac
     4141 acccaccgtc atgccgagcc tgcacatgcc gctgcagtcc gggtccgacc gggtcctggc
     4201 ggccatgcgc cggtcctacc gcagcgagcg gttcctcgcg atcatcgacg aggttcgcgc
     4261 ccagatcccc gacgccgcga tcacgaccga cctcatcgtg ggcttccccg gtgagaccga
     4321 ggccgacttc gaggcgacgc tcgatgtcgt gcgggcgtcg aggttctcca gcgcgttcac
     4381 cttccagtac tcggtccggc caggcacacc cgccgcgacg atgggcgacc aggtccccaa
     4441 gtccgtcgtc caggagcgct tcgaccggct catcgccgtg caggagcagg tcagctgggc
     4501 cgagaaccgc cggcaggaag gccggggagt cgaggtgctc gtggccaccg gcgagggccg
     4561 caagga
//
LOCUS       DATAIX010000708         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_259046, whole genome shotgun sequence.
ACCESSION   DATAIX010000708 DATAIX010000000
VERSION     DATAIX010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259046"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1189
                     /locus_tag="VHM65_10445"
     CDS             167..1189
                     /locus_tag="VHM65_10445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2706168.1"
                     /translation="MSSPLRTPRSSTATQPPTQQWAIEAVDLVKSYPGGRDKAPVRAL
                     DGLSLRVRPGEVFGLLGPNGAGKSTTVRILATLTMATSGRAMVAGHDVQRAPDDVRRA
                     IGLVSQKPSSAPIMTGRESIELAGRIQGLSRREARSRAADLLEQFGLADAANRLTKTY
                     SGGMTRRLDVAVGLANRPAVLFLDEPTTGLDPEARAQLWTEIRRLSTVDRTTILLTTH
                     YLDEADELADRLAIVDHGRVVAEGSPDELKRELKGDTVAVELADPVSGPVAAQRLARI
                     PGLSEVTQVGATLRARTDQGARAIPAVLASLEQAGLTVASATVARPSLDDVYRRHAGR
                     AFDGAA"
     gene            1189..1968
                     /locus_tag="VHM65_10450"
     CDS             1189..1968
                     /locus_tag="VHM65_10450"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2706169.1"
                     /translation="MTTLVTHSTLLTRRSLTALLRQPAYLAITLVQPMIWLLLFGQLF
                     STVVDIPGFAREGTFLEFLTPGVVAMTALFSASWAGTVYIEDMGLGVMDRLLASPVRR
                     GAMMNGTLIYQGIIAVAQTVAVLVVAVLAGARFGSPVGGFVVTIGSAVLLTVVFSALS
                     NAVALLVRQQEALIGISQMLALPMTFLSSAVMDVRLAPTWIERVATYNPLDWAVRASR
                     EALSATPDWGVVGSRLGLLAALALLMAWLATRAFGVYQRSV"
     gene            2053..>2248
                     /locus_tag="VHM65_10455"
     CDS             2053..>2248
                     /locus_tag="VHM65_10455"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX2706170.1"
                     /translation="MRVLGVDPGLTRCGLGVVDGGLGRSLTMVAVGVVRTRTDEPIER
                     RLLALQGEQTALDRLVRARAH"
BASE COUNT          302 a          822 c          780 g          344 t
ORIGIN      
        1 cccttgcgga cgcccgactg accaaacccc gggccaggtg ttgacgcacc tgacccgggg
       61 cctcttcctc gagccggtcc gatggcaggc caccagctga cccaaggcgc atcagtcagc
      121 ctagcggcac gccccggacc gactcctcgc caatcaagga gtcaccatgt cctcacccct
      181 gcgtacgccc cgatcctcga cggcgaccca gccgccgacc cagcagtggg ccatcgaggc
      241 cgtcgacctg gtcaagtcct accccggcgg tcgcgacaag gccccggtcc gggctctcga
      301 cggcctctcc cttcgcgtcc gacccggcga ggtgttcggc cttctggggc ccaacggtgc
      361 gggcaagtcc accaccgtgc gcatcctcgc gacactgacc atggcgacct cgggtcgtgc
      421 catggtggcc ggccacgacg tccagcgagc acccgacgac gtgcgccgtg cgatcggcct
      481 ggtctcgcag aagccgagtt cggcgccgat catgaccggc cgcgagagca tcgagctggc
      541 gggccgcatc cagggcctgt cgcgccgcga ggcacggagc cgggccgccg acctgctcga
      601 gcagttcggg ctcgccgacg cggccaaccg cctgaccaag acctacagcg gcggtatgac
      661 gcgccggctc gacgtcgccg tcggcctggc caaccgcccg gccgtgctct tcctcgacga
      721 gccgaccacg gggctggacc ccgaggcgcg agcgcagctg tggaccgaga tccgccggct
      781 cagcacggtc gatcggacga cgatcctgct cacgacgcac tatctcgacg aggccgacga
      841 gctggcggac cggctggcca tcgttgacca cggacgggtc gtggccgagg ggtccccgga
      901 cgagctcaag cgcgagctca agggcgacac cgtggcggtc gagctcgcgg acccggtctc
      961 gggcccggtc gcggcgcagc gcctcgcccg gatcccgggc ctgagcgagg tgactcaggt
     1021 cggtgccacc ctccgggccc gcaccgatca gggagcgcgc gccataccgg ctgtgctcgc
     1081 cagtctggaa caggccggac tgacggtcgc gtcggccacc gtcgcgcggc cctcgctcga
     1141 cgacgtctac cggcgccatg cggggcgcgc gttcgacgga gcagcctgat gaccaccctc
     1201 gtcacccact cgaccctgct caccaggcgc tcgctgacgg cgctgctacg ccagccggcc
     1261 tacctggcca tcaccttggt gcagccgatg atctggctgc tcctgttcgg acagctcttc
     1321 agcaccgtcg tcgacatacc gggcttcgcc cgtgagggga cgtttctcga gttcctgaca
     1381 cccggagtcg tcgccatgac ggccctgttc tccgcctcgt gggctgggac ggtctacatc
     1441 gaggacatgg ggctcggggt catggaccgg ctgctcgcct cgccggtccg gcgcggggcg
     1501 atgatgaacg ggacgctgat ctaccagggc atcatcgcgg tcgcccagac cgtggccgtc
     1561 ctcgtcgtgg cggtcctcgc gggagcccgg ttcggctcgc cggtgggcgg attcgtcgtg
     1621 acgatcggct cggcggtgct gctcaccgtc gtgttctcgg ccctgtccaa cgcggtggcc
     1681 ctgctcgtgc gccagcagga ggcgctcatc gggatcagcc agatgctggc tctgccgatg
     1741 accttcctgt catcggcggt catggacgtg cgtctcgccc cgacgtggat cgagcgggtc
     1801 gcgacataca acccgctcga ctgggccgtc cgcgcgagcc gggaggcgct gtcggcgacc
     1861 ccggactggg gtgtggtggg ctcgcggctc ggccttctgg cagccctggc actcctcatg
     1921 gcgtggctgg cgacccgggc gttcggcgtg taccaaaggt ccgtctgacc gcacggcgcg
     1981 tcgcgccgcg gccgaggacc gcgtgctgtg taacgtaaga gccgaacacg tgttcggagt
     2041 cggaggtatg gcgtgcgcgt cctcggcgtg gaccctgggt tgacccggtg cggcctcggt
     2101 gtcgtcgacg ggggcctggg ccgctccctg acgatggtcg ccgtcggagt cgtgcgcacg
     2161 cgcacggacg agccgatcga gcgccgtctg ctcgccctgc agggcgagca gacggcgctc
     2221 gatcggctcg tccgtgcgcg tgcgcacg
//
LOCUS       DATAIX010000709         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_236668, whole genome shotgun sequence.
ACCESSION   DATAIX010000709 DATAIX010000000
VERSION     DATAIX010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236668"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1381
                     /gene="ugpC"
                     /locus_tag="VHM65_10460"
     CDS             278..1381
                     /gene="ugpC"
                     /locus_tag="VHM65_10460"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX2706171.1"
                     /translation="MATVRFKDASRHFPGNPKPTIDKLNIEIEDGEFLVFVGPSGCGK
                     STCLRMLAGLEEVNGGQIFIGDRDVTNLSPKDRDVAMVFQNYALYPHMSVADNMGFAL
                     KIAGVAKDEIRKRVDEAAKILDLAPYLERKPKALSGGQRQRVAMGRAIVRSPQVFLMD
                     EPLSNLDAKLRVQTRTQIASLQRRLGVTTVYVTHDQTEAMTMGDRVAVLKDGYLQQCD
                     SPRYMYDHPDNVFVAGFIGSPAMNLLDVPVVDGGIKLGEHVVAVTREELAGAGSHVTF
                     GIRPEDMDLSAGTEGLELHVDVVEELGADAYIYGTPKGVQQLDTDGEGGAKPFLARVD
                     GRNPPKRGETVRIVPKPGHSHIFNADTGERLGT"
     gene            1466..>2240
                     /locus_tag="VHM65_10465"
     CDS             1466..>2240
                     /locus_tag="VHM65_10465"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HEX2706172.1"
                     /translation="MALEITVAGPTQAFLDLPWSTPLVEWPVETLAALPRGISRHVVR
                     FVRLDGRVIAVKEIKAELAEREYAMLRMLTRLDQPCVEPFGVVRGRVAEDGRPLDACL
                     ITRHLQFSLPYRALFSQQLRPDTAQRLVDALAVLLVQLHLTGFWWGDVSLSNTLFRRD
                     AGAFAAYLVDAETGELHLSLSHGQREHDLDIARVNIAGELMDLQAGGFLEDYVDPVDV
                     ATLILDRYHSLWTELTGLERFETGDRWRVDARIRRLNEQG"
BASE COUNT          352 a          831 c          706 g          351 t
ORIGIN      
        1 gcatcgactc gcggaacccc gcgatcttgc ggatgacgtc acagagctgt aacgggtgct
       61 cacccgcacc gggatggcga ggctcccgtg tctactccgg tgcgccgccg cgcgcatccg
      121 cgggcccctc gtccatgctc cacaccggcg tatggcgcgt cccccccttg gcgtgctgac
      181 tcgccatacg ccatgatgag ggtggccggg ggctcccggt cgccccttta caacggatcg
      241 tccggcacgt acctgccggt gaaggagttg ctgcatcatg gctactgtca ggttcaagga
      301 cgcctctcgg cacttcccgg gcaacccgaa gccgaccatc gacaagctga acatcgagat
      361 cgaggacggc gagttcctcg tcttcgtcgg cccctccgga tgcggcaagt ccacctgcct
      421 gcggatgctg gcgggcctcg aagaggtcaa cggcgggcag atcttcatcg gtgaccgcga
      481 tgtcaccaac ctgtccccca aggaccgtga cgtcgcgatg gtcttccaga actacgccct
      541 ctacccgcac atgtcggtgg ccgacaacat gggcttcgcc ctgaagatcg ccggtgtcgc
      601 caaggacgag atcaggaagc gggtcgacga ggccgccaag atcctggacc tggcccccta
      661 cctcgagcgc aagcccaagg ctctctccgg tggccagcgg cagcgcgtcg cgatgggccg
      721 ggcgatcgtg cgctcgcccc aggtcttcct catggacgag ccgctgtcca acctcgacgc
      781 caagctgcgg gtccagaccc gcacccagat cgcgtcgctg cagcggcgcc tcggcgtcac
      841 gaccgtctac gtcacgcacg accagaccga ggccatgacc atgggcgacc gggtcgccgt
      901 gctcaaggac ggctacctgc agcagtgcga cagcccgcgg tacatgtacg accacccgga
      961 caacgtcttc gtggccggct tcatcggctc ccccgcgatg aacctgctcg acgttcccgt
     1021 cgtcgacggt ggcatcaagc tcggcgagca cgtcgtcgcg gtgacccgtg aggagctggc
     1081 cggcgccggg tcgcacgtga ccttcgggat ccgccccgag gacatggatc tctcggcggg
     1141 caccgaaggg ctcgagctgc acgtcgacgt cgtcgaggag ctcggtgccg acgcctacat
     1201 ctacggcacg cccaagggcg tccagcagct cgacaccgac ggtgagggtg gggccaagcc
     1261 gttcctcgcc cgcgtcgacg gtcgcaaccc gcccaagcgg ggcgagacgg ttcgcatcgt
     1321 cccgaagccc gggcactcgc acatcttcaa cgccgacacc ggagagcgcc tcggcaccta
     1381 gccttcgcgc agacaggcaa gcagcggcgg cacatcctcc ccgggtgtgc cgccgcgaac
     1441 atgtcaccac cgaaggacca agcccgtggc cctagagatc accgtcgccg gcccgaccca
     1501 ggccttcctc gacctcccct ggtcgacccc cctggtggag tggccggtcg agaccctcgc
     1561 cgccctgccc cggggcatct cccggcacgt cgttcgattc gtgcgcctcg acgggcgggt
     1621 catcgccgtc aaggagatca aggccgagct cgccgagcgc gagtacgcca tgctgcgcat
     1681 gctgacccgt ctcgaccagc cctgtgtcga gccgttcgga gtggtccgcg gccgggtggc
     1741 cgaggacggg cgcccgctcg atgcctgtct catcacccgg cacctgcagt tctcgctgcc
     1801 ctaccgggcg ctgttcagcc agcagctccg gcccgacacg gcccagcggc tcgtcgacgc
     1861 actcgccgtc ctgctcgtcc agcttcatct caccggcttc tggtggggtg acgtctcgct
     1921 gtccaacacg ctcttccgcc gcgacgccgg cgcgttcgcg gcatacctcg tcgatgccga
     1981 gaccggcgag ctgcacctgt cgttgtccca cggccagcgc gagcatgacc tggacatcgc
     2041 ccgggtcaac atcgccggcg agctcatgga cctgcaggcc ggcggcttcc tcgaggacta
     2101 cgtcgacccg gtggacgtgg ctaccctcat cctcgaccgc taccactcgc tgtggaccga
     2161 gctgaccggt ctggaacggt tcgagaccgg cgaccggtgg cgcgtcgacg cccggatccg
     2221 ccgtctcaac gagcagggct
//
LOCUS       DATAIX010000710         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_297068, whole genome shotgun sequence.
ACCESSION   DATAIX010000710 DATAIX010000000
VERSION     DATAIX010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297068"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(300..2585)
                     /gene="purL"
                     /locus_tag="VHM65_10470"
     CDS             complement(300..2585)
                     /gene="purL"
                     /locus_tag="VHM65_10470"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX2706173.1"
                     /translation="MTTEVDTVEHAAATPEVEQPWAELGLKAAEYATIREILGRRPTS
                     AELAMYSVMWSEHCSYKSSKVHLAQFSENTTEEMNEKLLVGIGENAGVVDIGDGWAVT
                     FKVESHNHPSFVEPYQGAATGVGGIVRDIMSMGARPIAVMDSLRFGAIDHPDTHRVLP
                     GIVAGVGGYGNCLGLPNIGGEVVFDDCYQGNPLVNALCVGAMRVEDIHLAKASGAGNK
                     VVLFGARTGGDGIGGVSVLASETFDEGGPTKRPAVQVGDPFAEKVLIECCLDLYSARV
                     VAGIQDLGGAGLSCATSELASNGDGGMHIELDLVPLRDATLQPQEILMSESQERMMAV
                     VEPGKLDAFLEITRHWDVEATVIGEVTDGVHLVITWRGEVIVDVPPRSVAHDGPVYQR
                     PLARPAYLDVLTADDTTDLARPNGGGEQRHTLLTLLASPNLCDKSWVTGQYDRYVLGN
                     TALAQPDDAGVVRVDEATGHGVAISTDCNGRYVKLDPYAGAQLALAEAYRNVATAGAW
                     PLAVTDCLNFGSPEDPGVMWQFREAVRGLAEGCRFLGIPVTGGNVSFYNQTGDIAIHP
                     TPVVGVLGVMEDVGRRTASGFRQAGHAVFLLGTTRAELDGSEWAHVVHGHLGGRPPQV
                     DLAGEMRLAQVLIEGAEEHALASAHDLSEGGLAIGLAEAVLRFGVGARVSIDELCDRD
                     GIDSFTALFSESAARAVVAVKESRVRQLAQLCAANSVPLVRIGEVTQPGDGLEFVGHF
                     AVSLDELRAVHTATLPAIFGA"
BASE COUNT          400 a          961 c          947 g          383 t
ORIGIN      
        1 ccggtcccga ctggggtccg ctcaggcagc gagctcgctc gtctggcggc gggagcgcgc
       61 gccttgaccg acagcgacag cgcgctgtcc ccgaatccgc ctgaccctgg cgcctggcgc
      121 cgccgctaac cttcgcgccg acccttcgcg ccgacccttc gcgccggcga tgcgtcatcc
      181 gagcgtcgat tcgggcgctg aaacccaccc ctggggcgac ttccagccac cgaacgccac
      241 gtcgggcgct gaaacccgcc cctggggcga cttccagccc gcggcaacgc gctaacccgt
      301 caggcgccga agatcgccgg cagggtggcc gtgtggaccg cgcgcagctc gtcgaggctc
      361 actgcgaagt ggccgacgaa ctcgaggccg tcaccgggct gggtgacctc gccgatgcgg
      421 acgagcggca ccgagttggc cgcgcacagc tgggccagct ggcgcacccg cgactccttg
      481 accgcgacga ccgcccgggc cgccgactcg ctgaacagcg ccgtgaacga gtcgatcccg
      541 tcgcggtcgc acagctcgtc gatgctgacc cgtgcaccga ctccgaagcg cagcaccgcc
      601 tcggcaagcc cgatcgcgag gccgccttcg gacaggtcgt gggccgaggc gagggcgtgc
      661 tcctcggcac cctcgatgag cacctgggca aggcgcatct caccggcgag gtcgacctgc
      721 ggcggccgcc cgccgagatg cccatggacg acatgggccc actccgaccc gtcgagctcg
      781 gcgcgggtcg tcccgagcag gaacaccgcg tggccggcct ggcggaagcc cgacgccgtc
      841 cgccgcccga cgtcctccat gaccccgagc acgccgacga cgggcgtcgg gtggatcgcg
      901 atgtcgccgg tctggttgta gaacgacacg ttgccgccgg tgacggggat ccccaggaac
      961 cggcatcctt cggccagccc gcgcacggcc tcacggaact gccacatgac acccgggtcc
     1021 tccggtgagc cgaagttgag gcagtcggtg accgcgagcg gccacgcccc ggcggtcgcc
     1081 acgttgcggt aggcctcggc gagcgcgagc tgcgcaccgg catacgggtc gagcttgacg
     1141 tagcggccgt tgcagtcggt cgagatcgcc acgccgtgcc cggtcgcctc gtcgacgcgg
     1201 acgacccccg cgtcgtccgg ctgggccagc gcggtgttgc ccaggacgta gcggtcgtac
     1261 tggccggtga cccacgactt gtcgcacagg ttcgggctcg cgagcagggt gagcagggta
     1321 tggcgctgct caccaccgcc gttgggccgg gcgaggtcgg tggtgtcgtc ggcggtcagg
     1381 acgtcgaggt atgccgggcg cgccagcggc cgctggtaga ccgggccgtc gtgggcgacc
     1441 gagcgcggcg ggacgtcgac gatgacctcg ccgcgccagg tgatgacgag gtgcacgccg
     1501 tcggtgacct ccccgatcac cgtggcctcg acatcccagt gccgggtgat ctcgaggaac
     1561 gcgtcgagct tgcccggctc gacgaccgcc atcatccgct cctgcgactc ggacatgagg
     1621 atctcctgcg gctgcagggt ggcatcgcgc aggggcacga ggtcgagctc gatgtgcatg
     1681 ccgccgtcac cgttggaggc gagctcgctg gtggcacagg acaacccggc gccgccgaga
     1741 tcctggatcc ccgcgacgac gcgcgcggaa tacaggtcca ggcagcactc gatgaggacc
     1801 ttctcggcga acgggtcacc gacctggacg gccgggcgct tcgtgggacc accctcgtcg
     1861 aaggtctccg aggcgagcac cgagacgccc ccgatgccgt cgccgccggt cctggcgccg
     1921 aagagcacga ccttgttgcc cgcgcccgag gccttggcga ggtggatgtc ctcgacccgc
     1981 atcgcgccga cgcagagggc gttgacgagc gggttgccct ggtagcagtc gtcgaagacg
     2041 acctcgccgc cgatgttggg caggccgagg cagttgccgt agccgccgac cccggcaacg
     2101 atcccgggca gaacccgatg cgtgtccggg tggtcgatgg cgccgaaccg caacgagtcc
     2161 atgacggcga tcgggcgcgc acccatcgac atgatgtcgc ggacgatgcc cccgaccccg
     2221 gtggccgcgc cctggtaggg ctcgacaaac gagggatggt tgtggctctc gaccttgaag
     2281 gtcaccgccc agccgtcccc gatgtcgacc acgcccgcgt tctcgccgat gccgaccagg
     2341 agcttctcgt tcatctcctc ggtggtgttc tcgctgaact gtgcgaggtg gacctttgag
     2401 gacttgtacg agcagtgctc ggaccacatg accgagtaca tcgccagctc ggcgctcgtc
     2461 ggccgacgcc cgaggatctc gcggatggtg gcgtactcgg ctgccttcag acccagctcg
     2521 gcccagggct gctcgacctc tggggtggcg gcggcgtgct cgacggtgtc gacctcggtc
     2581 gtcatgaggc cagcacctgc tccatgacgg aggtgaagaa cggcaccccg tcgagcgacg
     2641 gcccgtagcc ggtctcgacg cagtgctcgg ggtggggcat gagcccgacg a
//
LOCUS       DATAIX010000711         5262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_237267, whole genome shotgun sequence.
ACCESSION   DATAIX010000711 DATAIX010000000
VERSION     DATAIX010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237267"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VHM65_10475"
     CDS             <1..190
                     /locus_tag="VHM65_10475"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX2706174.1"
                     /translation="EPGSVRLNETVPLAGAVIAEVQTLRDRHTVIAPHGGRVIEWLVT
                     DGDPVSPGQPLLRLHPQV"
     gene            196..1221
                     /locus_tag="VHM65_10480"
     CDS             196..1221
                     /locus_tag="VHM65_10480"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX2706175.1"
                     /translation="MTPMKRSPKASGRLSPTSGAQHARIWGVGGYRPERVVTNEEICT
                     WIDSSEEWIRERSGIESRRFAAADETVTDMAEIAARQALAAAGVSPEQVGTILVATVT
                     HPYQTPASAPLLAHRLGGHAAAFDISAACAGYCYGISLANDMVRGGSAEFVLVVGVEK
                     LSDFTDRGDRGTAFIFGDGAGAAVVGPSDFPGIGPTVWGSDGDQWTTISQRESWIEVH
                     DKNLSWPAIGMAGQSVFRWAVWQMAPVAQAAIDKSGITADDLDAFVPHQANMRIIDAL
                     VKQLRLPADIAVARDIAQTGNTSAASIPLATERMLREGDAPSGGLALQIGFGAGLVYA
                     AQVVVLP"
     gene            1356..1604
                     /locus_tag="VHM65_10485"
     CDS             1356..1604
                     /locus_tag="VHM65_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017489700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX2706176.1"
                     /translation="MAQTEQEILEGLAEIVNEETGLDTASVELDKSFTDDLDIDSLSM
                     MTIVVNAEEKFGVRIPDDEVKNLRTVRDAVSFIVGAQG"
     gene            1683..2933
                     /gene="fabF"
                     /locus_tag="VHM65_10490"
     CDS             1683..2933
                     /gene="fabF"
                     /locus_tag="VHM65_10490"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX2706177.1"
                     /translation="MTSPHDQRIVVTGLGATTPVGGTVPQTWEAITAGRSGARTMEFD
                     WVSTYELPVTFAATIHTQPDQVLSRVETRRLDPSSQYALIAAREAWADAGAPEVEPER
                     LAVAVGSGIGGVWTLLSQWDTLKEKGPRRVFPLAVPMLMPNGPAAAVSLELGARAGAH
                     TPVSACASGAEAMGFGLEMIRSGRADIVVCGGTEAAVHALPIAGFAAMQALSTRNDDP
                     ERASRPYDTARDGFVLGEGAAVIVLERADHARARGATIYAELASVGMSADAHHIAAPE
                     PEGAGAARAMVEAVERAGLTPADIVHINAHATSTPVGDVAESNAIRRAFGGAADAMAV
                     SATKSMTGHLLGAAGAIEAVLTILAIRDRIAPATINLENRDPQIDLDVVHGSARRLPA
                     GDIAALNNSFGFGGHNVALVVRNA"
     gene            2933..4384
                     /locus_tag="VHM65_10495"
     CDS             2933..4384
                     /locus_tag="VHM65_10495"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX2706178.1"
                     /translation="MSARTPSTASVQAAASARTKVPREQDPRNPNTRLGAFFDPGTIE
                     LITPDDGSGMLAAIGTVSGTPAVAFCSDATFQGGALGIDGCQAIVTAYERALFEGVPV
                     VGIWHSGGARLAEGVVSLHGFGLVFAIMTRASGKIPQLSIVIGPAAGGGAYGPALTDV
                     VILGPEGRVFVTGPDVVRSVTGEQVDALRLGGPEPHGRRSGVVHVVTETTQEAFERGR
                     LLCTLLAAQGTLEVESVQDRDLARTLPDSAKRAYDVHPLVADVLDDEAGSVELHPRWA
                     PNICTTLGRFGGRTVGVVANNPMRLGGCLDSSAAEKAARFVRMCDAFGIPLVVLVDVP
                     GYLPGVGQEWDGVVRRGAKLLHAFAEATVPRVTLITRKTYGGAYIAMNARSLGASRVF
                     AWPTAEVAVMGAVAAVRILHRRRLAEVDEEDRAGVELELAGEHALLSDGLAKAVDIGV
                     VDEIVEPHKSRSALAAAIVAAPQRRGSHGNIPL"
     gene            4522..>5262
                     /locus_tag="VHM65_10500"
     CDS             4522..>5262
                     /locus_tag="VHM65_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX2706179.1"
                     /translation="MLSSTAAIDLWGDCGDLPAALRVHDDAVEVLTRIWHPLFQAQLR
                     LSALLLGQLASHVQRVPTADRAGLLDRGRITAETAVRVWDQSQQWPGNDGPESAAWQS
                     RAAAEALRLRWLGGAEIEPGALAYAWRESVAAFETYGHIFEAARSRARLGAVLLAAGE
                     PAAGEALRAALETARQLGAKPLRAEIRAVGGADQPPVARPADRYGEALTGREKEILGL
                     LALGRSNRQIGTQLFISAKTASVHGSNIL"
BASE COUNT          754 a         1950 c         1828 g          730 t
ORIGIN      
        1 cgagccgggc tcggtcaggt tgaacgagac cgtccccttg gcgggcgccg tcatcgccga
       61 ggtccagacc ctgcgcgacc gccataccgt gatcgcgccg cacggcggcc gggtcatcga
      121 atggcttgtc accgacggcg atccggtgtc ccccggtcag cccctccttc gacttcaccc
      181 tcaggtgtga cacctatgac ccccatgaag cgcagtccca aggcatctgg ccggctctcc
      241 cccaccagcg gcgcccagca cgcccggatc tggggcgttg gaggctaccg tcccgaacgg
      301 gtcgtcacca acgaggagat ctgcacctgg atcgactcca gcgaggagtg gatccgcgag
      361 cgctcgggca tcgagagccg ccggttcgcc gctgccgacg agacggtgac cgacatggcc
      421 gagatcgcgg cacgccaggc cctggcggct gccggggtga gcccagagca ggtgggcacc
      481 attctcgtcg ccaccgtgac ccatccctac cagaccccgg cgtcggcccc gctactggca
      541 catcgtctcg gcggccatgc ggcggccttc gacatctcgg ccgcctgtgc tgggtactgc
      601 tacggcatct ccctcgccaa cgacatggtc cgtggcggga gcgccgagtt cgtcctcgtc
      661 gtcggggtgg agaagctctc cgacttcacc gaccgcggtg accgggggac ggcgttcatc
      721 ttcggtgacg gcgcgggggc cgccgtggtc ggcccgtccg acttcccggg catcgggccc
      781 acggtctggg gctccgatgg ggaccagtgg acgacgatct cccagcgcga gtcgtggatc
      841 gaggtccacg acaagaacct cagctggccg gccatcggga tggcgggcca gtccgtcttc
      901 cggtgggccg tctggcagat ggcgcccgtc gcccaggccg cgatcgacaa gtccgggatc
      961 accgccgacg acctcgacgc cttcgtgccg caccaggcca acatgcggat catcgacgcg
     1021 ctggtcaaac agctcaggct gcctgcggac atcgcggtgg cccgtgacat cgcgcagacc
     1081 ggcaacacct cggccgcctc gatccccctg gccaccgagc gcatgctgcg cgagggcgac
     1141 gcgcccagcg gcggcctcgc gctgcagatc ggcttcgggg ccggtctcgt gtatgccgca
     1201 caggtggtcg tgcttcccta ggcgtccact cgccataccg gatcctccgg caggccgacg
     1261 aactcccgcg tcccggcggg cactctcggg acacagcagg acccagcacc gacccacagc
     1321 cagcaccgac ccacagctca cccaaggagc accacatggc acagaccgag caggagatcc
     1381 tcgagggtct cgccgagatc gtcaacgagg agaccggcct ggacacggcg tcggtcgagc
     1441 tcgacaagtc gttcacggac gacctcgaca tcgactcgct gtcgatgatg acgatcgtcg
     1501 tcaacgccga ggagaagttc ggtgtccgca tccccgacga cgaggtcaag aacctgagaa
     1561 ccgtccgcga cgcggtctcc ttcatcgtcg gggcccaggg ctgagccgcc cgccgacgcg
     1621 ctgtcgcaag cccatctgcc cacctgcaca gtcccgcacc gccagccgag gagcacgacc
     1681 ccatgacgtc cccccatgac cagcgcatcg tcgtgaccgg actgggtgcc acgacgcccg
     1741 tcggcggcac cgtccctcag acctgggagg cgatcaccgc gggccgctca ggcgcacgga
     1801 ccatggagtt cgactgggtc agcacctacg agctgccggt gaccttcgcg gcgacgatcc
     1861 acacccagcc cgaccaggtg ctctctcggg tggagacgcg ccgcctcgac ccgtcctcgc
     1921 agtacgccct catcgcagcg cgcgaggcgt gggcggatgc cggggcgccc gaggtcgagc
     1981 ccgagcgcct cgccgtggcg gtcgggtctg gcattggcgg ggtctggacc ctgctgagcc
     2041 agtgggacac gctcaaggag aagggcccgc ggcgcgtctt cccgctggcc gtgccgatgc
     2101 tcatgcccaa cgggccggcg gccgccgtct cgctcgagct cggagcccgg gctggggccc
     2161 acacccccgt cagcgcctgc gcatcgggcg ccgaggcgat ggggttcggt ctggagatga
     2221 tccgcagcgg ccgcgccgat atcgtcgtct gcggcggcac cgaagccgcc gtccacgcgc
     2281 tccccatcgc gggtttcgcg gcgatgcagg cactgtcgac ccgcaacgac gaccccgagc
     2341 gggcctcacg cccctatgac acggcccggg acgggttcgt cctcggcgag ggtgcggccg
     2401 tcatcgtcct cgaacgcgcc gaccacgcgc gggcgcgcgg ggcgacgatc tacgccgagc
     2461 tggccagcgt gggtatgtcg gcggatgccc accacatcgc cgctcccgaa cctgaggggg
     2521 cgggcgcggc cagggcgatg gtcgaggcgg tcgagcgggc cggcctgacg ccggccgaca
     2581 tcgtgcacat caacgcgcac gccacgtcga ccccggtcgg cgacgtcgcc gagtccaacg
     2641 cgatccgccg ggccttcggc ggcgcggccg acgcgatggc ggtcagcgcc accaagtcga
     2701 tgaccgggca cctgctcggc gcggccggcg cgatcgaggc ggtgctgacc atcctcgcca
     2761 tccgcgaccg gatcgccccc gcgacgatca acctggagaa cagggacccg cagatcgacc
     2821 tcgacgtcgt gcatggcagc gcccgccggc tcccggcggg tgacatcgcg gccctgaaca
     2881 actccttcgg cttcgggggc cacaacgtcg ccctcgtcgt ccggaacgcc tgatgtcggc
     2941 acgcaccccg tcgaccgcgt ccgtgcaggc cgccgcctcg gcgcgcacca aggttccccg
     3001 ggagcaggac ccgcgcaacc ccaacacccg gctcggcgcg ttcttcgacc cgggcaccat
     3061 cgagctgatc accccggatg acgggtcggg catgctcgcg gcgatcggga cggtgtcggg
     3121 gacacccgca gtggcgttct gctcggacgc caccttccag ggtggtgccc tcggcatcga
     3181 cgggtgccag gccatcgtca cggcctacga gcgggcgctg ttcgaagggg tgccggtcgt
     3241 cggcatctgg cactcgggtg gtgcccgcct cgccgagggg gtcgtgtcgc tgcacggctt
     3301 cggcctggtc ttcgccatca tgacgcgggc gtcgggcaag atcccccagc tctcgatcgt
     3361 catcggcccc gcggccggtg gcggggccta cggcccggcg ctgaccgacg tcgtcatcct
     3421 cgggcccgag gggcgggtct tcgtcactgg tcccgatgtc gtgcgctcgg tcaccggtga
     3481 gcaggtcgac gcgctgcgcc tgggcggccc ggagccgcac ggtcgccgct ccggcgtcgt
     3541 ccatgtcgtc accgagacga cccaggaggc gttcgagcgg gggcgcctgc tctgtaccct
     3601 gctcgccgcg cagggcaccc tcgaggtcga gtccgtccag gaccgcgacc tggcccggac
     3661 cctgcccgac tcggccaagc gggcgtatga cgtccatccc ctcgtcgccg acgtgctcga
     3721 cgacgaggcc ggctcggtcg agctgcatcc ccgctgggcc cccaacatct gcaccaccct
     3781 cggccggttc gggggccgca cggtcggcgt cgtcgccaac aacccgatgc gcctcggcgg
     3841 ctgcctggac tcctccgcgg ccgagaaggc cgctcggttc gtgcggatgt gcgacgcgtt
     3901 cggcataccc ctcgtcgtgc tcgtcgacgt tcccggctac ctccccggcg tcgggcagga
     3961 gtgggacggg gtcgtgcgcc gtggcgccaa gctgctccac gccttcgccg aggccaccgt
     4021 gccccgggtc accctgatca cccgcaagac gtatggcggc gcctacatcg ccatgaacgc
     4081 ccgctccctg ggcgcctccc gcgtgttcgc ctggccgacc gccgaggtgg ccgtcatggg
     4141 agcggtggcc gcggtgcgga tcctgcaccg gcgccggctg gccgaggtcg acgaagagga
     4201 ccgcgccggt gtcgagctcg agctggccgg cgagcacgcg ctgctgtccg atgggctggc
     4261 caaggcggtc gacatcggcg tcgtcgacga gatcgtcgag ccccataaga gccgctcagc
     4321 tctcgcggcc gcgatcgtgg cggcaccaca gcgcaggggc agccacggca acattccgct
     4381 gtaaccccct ctcggaggcc aggtcgccac ctcgcccagt cgcgagcgga ctccgagcgg
     4441 ggatgcgggc gtggtgaccc ggccggactg gatggcttcc cccaggtccg cgagtggtgg
     4501 gaggccgacg gcctggcagc ggtgctgagc agcaccgctg ccatcgacct gtggggcgac
     4561 tgcggagacc tcccggccgc gctgcgcgtc cacgacgacg cggttgaggt cctgacgcgc
     4621 atctggcacc cgctcttcca ggcccagctc cggctcagtg cattgctgct cggacagctc
     4681 gccagccacg tgcagcgggt ccccacggcg gatcgtgcgg ggctgctcga ccgcggcagg
     4741 atcaccgccg agaccgcggt ccgcgtgtgg gaccagtccc agcagtggcc tggcaacgac
     4801 gggcctgagt cggccgcctg gcaatcccgt gccgcggcgg aggcactgcg gttgaggtgg
     4861 ctgggcggcg ccgagatcga gccaggcgcg ctggcctacg catggcggga gtcggtggca
     4921 gcgttcgaga cctacggcca catcttcgag gcggctcggt cacgggcacg actgggcgcg
     4981 gtgctcctgg ccgccggtga accagccgcc ggggaggcgc tccgggcagc gctggagacg
     5041 gcacgccagc tcggcgccaa gcccctgcgg gccgagatcc gggcagtcgg cggggctgac
     5101 caaccgccgg tcgccagacc ggccgaccgg tatggcgagg cgctgaccgg gcgcgaaaag
     5161 gagatcctgg gactgctggc gctcgggcgc agcaaccgcc agatcggcac ccagctgttc
     5221 atcagtgcca agaccgccag cgtgcacggc tccaacatcc tg
//
LOCUS       DATAIX010000712         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_259289, whole genome shotgun sequence.
ACCESSION   DATAIX010000712 DATAIX010000000
VERSION     DATAIX010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259289"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VHM65_10505"
     CDS             complement(<1..815)
                     /locus_tag="VHM65_10505"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX2706180.1"
                     /translation="MAGGQDHALAPGSADDTVGPPDGPSYELVAMGRTGVDLYPLQSG
                     VGLEDVSTFQKFLGGSATNVSVAAARYGRRTALVTRTGQDPFGRYVRRALRELGVDDV
                     FVSAVEGPPTPVTFCEIFPPDDFPLYFYRYPTAPDLMIEPGELPLEAIAAAGIFWVTV
                     TGLSQEPSRAAHHRALAARGRRRHTVLDLDYRPMFWADQAEAHEQVSIVLDLVSVAVG
                     NCDECEVAGGEREPHRAADALLERGVELVIVKQGPEGVLAATRYERLEVAPFPV"
     gene            1040..>2524
                     /locus_tag="VHM65_10510"
     CDS             1040..>2524
                     /locus_tag="VHM65_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706181.1"
                     /translation="MALTVGGLAAPTAAAAAEPLAFVTVSVGSGHGCGITPGADLWCW
                     GASRWGQVGDGTQQTRSAAVQIGIGSHWRQVDLGPTSTCAVKDDGSLWCWGSRYAAPG
                     RAESSLTPLRVGATRTWRSVSVGGTHACAVGEDASLWCWGYNESGQLGIGSHETTVEP
                     ARVDDRTDWSSVSAGGNHTCAVRTPGSLWCWGTTAQSQLGLPVDTWRVRPQRVAATSA
                     WAQVSAGADHTCAIRTDGLLFCWGTNTFGQIGVGRNETEPAPRQVGTSRWASVSSATS
                     YTCGQRTTGEARCWGSNSVAQLGDGTTVDCGTPVLVIGRPWTVVSPGAQLTCGLRADG
                     TGGCWGVNTSAGSGAGFTSIGTYSVLSPWSLPGTTWTTVDGSCGLRAEGSLWCWGANA
                     RGTVGDGTTTDAPAPVQVGVGSTWSSVTGDEHRCALRSDGTLWCWGNNRQGQLGDGTT
                     GGQRSTAVQVGAERTWATVSAAGLRTCAVATDGSLWCWGRGPGMG"
BASE COUNT          327 a          898 c          944 g          355 t
ORIGIN      
        1 acggggaagg gcgcgacctc gaggcgctcg tagcgggtcg cggcgaggac tccctcgggg
       61 ccctgcttga cgatgacgag ctcgacgccg cgctccagga gcgcgtccgc ggcccggtgg
      121 ggttcgcgct ccccgcccgc cacctcgcac tcgtcgcagt tgccgaccgc gacactgacc
      181 aggtcgagca cgatggacac ctgctcgtgc gcctcggcct ggtcagccca gaacatcggg
      241 cggtagtcga ggtcgagcac ggtatgacgg cgcctccccc gggccgccaa cgcgcggtgg
      301 tgggcggccc ggctcggttc ctggctcagg ccggtgaccg tgacccagaa gatcccggcc
      361 gccgcgatcg cctcgagcgg gagctctccg ggctcgatca tgaggtcggg cgccgtcggg
      421 tagcggtaga agtagagcgg gaagtcgtcc ggggggaaga tctcgcagaa ggtcaccggt
      481 gtcggcggtc cctcgaccgc gctgacgaac acgtcgtcga caccgagctc gcgcagcgcg
      541 cgcctgacgt agcggccgaa gggatcctga cccgtgcggg tgaccaacgc ggtccggcgg
      601 ccatatcgcg cggcggcgac gctgacgttg gtcgcactcc cgccgaggaa cttctggaac
      661 gtcgagacgt cctcgagccc gaccccgctc tgcagcgggt agaggtccac gccggtgcgc
      721 cccatggcga ccagctcgta tgacggcccg tccggggggc cgaccgtgtc gtcagccgag
      781 cccggggcca gcgcgtggtc ctggccgccc gccatcctcc acccgcttcc cgtgacctcc
      841 cgagggacgt cctgtccgcc accgttctat cgcgtcgcgg cgcgaaccgc gagcaccggt
      901 ggccggggct tcggccgaac agggtcgccg gacgatccgt cctgctagcc tgtgcgcgcc
      961 gcgccagggt gtcgcgggct gtccggggac tggggaacgt caccatgagt cgagcgtcat
     1021 cttcgcttac cttgccatgg tggccctgac ggtgggaggc ctggccgctc ccaccgcggc
     1081 cgcagccgcc gaaccgctgg ccttcgtgac ggtctccgtg gggtcgggtc acggttgcgg
     1141 gatcaccccc ggggccgacc tgtggtgctg gggcgccagc cgctggggcc aggtcggcga
     1201 cggcacccag cagacccggt ccgcggccgt ccagatcggc atcggcagcc actggaggca
     1261 ggtcgacctg gggcccacgt ccacctgcgc ggtcaaggac gacgggtcct tgtggtgctg
     1321 ggggtcgcgc tatgccgcac cgggacgggc cgagtcttcg ctgactccgc tgcgcgtggg
     1381 agccacccgg acctggcgct cggtatcggt gggcggcacc cacgcctgtg cggtgggcga
     1441 ggacgccagc ctctggtgct ggggctacaa cgagagcggg cagctcggga tcggcagcca
     1501 cgagaccacc gtcgagccgg cccgggtcga cgaccgcacc gactggtcga gcgtctcggc
     1561 cggtggcaac cacacctgcg ccgtgcggac cccgggctcg ctgtggtgct ggggcaccac
     1621 cgcccagtcc cagctcggcc tcccggtcga tacgtggcgg gtgcgcccgc agcgggtcgc
     1681 agcgaccagc gcgtgggccc aggtttccgc aggggccgac cacacctgcg ccatccgcac
     1741 cgacggtctg ctcttctgct gggggaccaa caccttcggc cagatcggcg tgggccgcaa
     1801 tgagaccgag ccagcacccc ggcaggtcgg gacgtcgagg tgggcttcgg tgtcttccgc
     1861 gacgtcgtac acctgcggtc agcgcacgac cggtgaggcg cgctgctggg gcagcaactc
     1921 cgtggcccag ctcggtgacg ggacgaccgt ggattgcggc actccggtcc tcgtcatcgg
     1981 ccggccgtgg accgtcgtgt cgccgggagc ccagctcacc tgtgggctgc gtgccgacgg
     2041 cacgggtggc tgctggggag tcaacacctc cgcggggtcc ggcgccggct tcacctcgat
     2101 cggcacctac tccgtcctgt ccccgtggtc cctacccggg acaacctgga cgacggtcga
     2161 cggctcgtgt ggtctgcggg ccgagggctc actgtggtgc tggggcgcca atgcgcgcgg
     2221 gacggtcggc gatgggacca cgaccgacgc cccggccccg gtccaggtcg gggtcggctc
     2281 gacgtggagc tccgtgaccg gcgacgagca tcgatgcgcc ctgcgctccg acggcaccct
     2341 ctggtgctgg ggcaacaacc ggcagggcca gctcggggac ggcacgaccg ggggtcaacg
     2401 cagcactgcg gtccaggtcg gcgccgagcg cacctgggcg acggtgagcg ccgccggcct
     2461 gcgcacctgc gccgtcgcga cggacgggtc gctgtggtgc tggggacggg ggccgggaat
     2521 gggc
//
LOCUS       DATAIX010000713         3734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_237594, whole genome shotgun sequence.
ACCESSION   DATAIX010000713 DATAIX010000000
VERSION     DATAIX010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237594"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1962
                     /locus_tag="VHM65_10515"
     CDS             148..1962
                     /locus_tag="VHM65_10515"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX2706182.1"
                     /translation="MTAQTTNAPTTHAGLTAWVAEVAELTQPDAIQWVTGEPAEIDAL
                     NQALVDGGTFVRLNDDIKANSFWCASDPSDVARVEDRTYIASLKKEDAGPTNNWVDPA
                     ELKATMSDLYRGCMQGRTMYVIPFVMGHLDATVPMFGVEITDSAYVVVNMNIMARIGA
                     PVLAKMEEIDADFVKGLHSVGAPLTQGQADVPWPCNTTKYIAHFPETREIWSFGSGYG
                     GNALLGKKCYALRIASAMARDDGWMAEHMLILKLTSPAGTVHYVAGAFPSQCGKTNLA
                     MIEPTVDGWKAEMIGDDIAWMRFGPDGRLYAVNPEFGLFGVAPGTGMNTNPVAMRTIE
                     RGNSIFTNVARTDDGDVWWEGMSKVKPAHLTDWKGRDWTPDNGELSSHPNSRFTTPAK
                     QCPMIADEYDNPDGVPIDAILFGGRRASTVPLVYESRDWTHGTFVGATLSSETTAAAT
                     GAVGVVRRDPMAMLPFIGYHAGDYMNHWLEIGKAADASKLPRIFQVNWFRKDEDGGWL
                     WPGFGDNSRVLKWVFERLEGVAEAVETPVGLVPAPGALDTTGLDMSDEDVAKALAVNT
                     EDWLKEIPLIEEWFAKFGDKLPAQLRAELDGLKERLGA"
     gene            2088..3398
                     /locus_tag="VHM65_10520"
     CDS             2088..3398
                     /locus_tag="VHM65_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX2706183.1"
                     /translation="MPANPRYLPAPDTAVVHPDWARTASIYQLNTRHFTTEGTFAAAR
                     AHLPRLKELGVSILWLMPIHEIGRTKRKGTLGSPYAVKDYLSVNPEFGTVEDLKAFVD
                     AAHELGLHVLLDWVANHTAWDNHLVTDHPEWYARDWKGDFCPTPWWDWDDIIDLDYSH
                     PGLREYMTEALCYWVREVGIDGYRCDVAGFVPIDFWNTARATLEEIKPVFLLAEWEDR
                     DLHVGAFDMTYAWSWNMTMHHIASGRADVTDLRVFYAWDAKAYQRDSIRMMFVANHDK
                     NAWEGTESEQFGEMLDSAVVLSVVGKGMPLVFNGQEAGSDKRFQFFEKDLIDWREHPM
                     GDFYRRLLVLKRDTSALWNGAWGAPMVNVPNDAESAVLSFVRQDEASKVFAVFNLSPL
                     ARGVCCREHLCHGTYRDFFSGTEVVVGDGTSLALPAWGHRLLLA"
     gene            3480..>3734
                     /locus_tag="VHM65_10525"
     CDS             3480..>3734
                     /locus_tag="VHM65_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706184.1"
                     /translation="MISPPAQRAGPPHAAVDDGDERRHEHGPDEQRVEQDAEPTMIPI
                     CPSAMSGSTPSTVNTAASRIPALVMTPPVTATARTMPAVVP"
BASE COUNT          642 a         1364 c         1195 g          533 t
ORIGIN      
        1 acctttgaca gtgcgccccc ccgcgaggga cgcgtaccgt gatcccacca ggtccatgcg
       61 caggcgcggg tccgccatcg gtcacgcgca aggcgctcca ttcatgccgt gcagacgaca
      121 ttgcacgcga aagggatgac acgagtcatg acagcccaga ccaccaacgc tcctacgacg
      181 catgccggcc tgaccgcctg ggtcgccgag gtcgccgagc tcacccagcc cgacgcaatc
      241 cagtgggtca ccggggagcc agccgagatc gacgccctga accaggccct cgtcgacggc
      301 gggacgttcg tccgcctcaa cgacgacatc aaggccaact ccttctggtg cgcctccgac
      361 cccagcgatg tcgctcgggt cgaggaccgc acctacatcg cctccctcaa gaaggaggac
      421 gccggcccga cgaacaactg ggtcgacccc gccgagctca aggcgacgat gagcgacctc
      481 taccgcggct gcatgcaggg ccgcacgatg tacgtcatcc cgttcgtcat gggccacctc
      541 gacgccaccg tcccgatgtt cggtgtcgag atcaccgact cggcctacgt cgtcgtcaac
      601 atgaacatca tggcccgcat tggcgcgccg gtcctggcca agatggaaga gatcgacgcc
      661 gacttcgtca agggcctgca ctcggtcggt gcaccgctca cgcagggcca ggccgacgtg
      721 ccctggccgt gcaacaccac gaagtacatc gcccacttcc cggagacacg cgagatctgg
      781 tccttcgggt cggggtatgg cggcaacgcg ctgctcggca agaagtgcta cgccctgcgc
      841 atcgcatcgg ccatggccag ggacgacggc tggatggccg agcacatgct catcctcaag
      901 ctcacctccc ccgcgggcac ggtccactac gtggccggcg ccttccccag ccagtgcggc
      961 aagaccaacc tcgccatgat cgagccgacg gtagacggct ggaaggccga gatgatcggg
     1021 gacgacatcg cgtggatgcg cttcggcccc gacggccggc tgtatgccgt gaaccccgag
     1081 ttcggcctct tcggcgtggc acccggcacg ggcatgaaca cgaacccggt ggccatgcgg
     1141 accatcgaga ggggcaactc catcttcacc aacgtcgccc gcaccgacga cggcgacgtg
     1201 tggtgggaag gcatgagcaa ggtcaagccc gcgcacctga ccgactggaa gggccgcgac
     1261 tggaccccgg acaacggcga gctgtcgagc cacccgaaca gccgcttcac caccccggcc
     1321 aagcagtgcc cgatgatcgc cgacgagtac gacaaccccg acggcgtgcc gatcgacgca
     1381 atcctcttcg gtggccggcg ggcctcgacc gtgccgctcg tctacgagtc gcgcgactgg
     1441 acccatggca cgttcgtcgg cgcgacgctg tcgtcggaga cgaccgctgc cgcgaccggg
     1501 gcggtcggcg tcgtacggcg cgacccgatg gcgatgctgc ccttcatcgg ctaccacgcc
     1561 ggtgactaca tgaaccactg gctcgaaatc ggcaaggcgg ccgacgcctc caagctgccg
     1621 cggatcttcc aggtcaactg gttccgcaag gacgaggacg gcggctggct ctggccaggc
     1681 ttcggcgaca acagccgggt gctcaagtgg gtcttcgagc ggctcgaggg tgtcgccgag
     1741 gccgtcgaga cccccgtggg tctcgtgccc gcgccaggtg ccctcgacac gaccggcctg
     1801 gacatgtccg acgaggacgt ggccaaggcc ctcgcggtca acaccgagga ctggctcaag
     1861 gagatcccgc tcatcgagga gtggttcgcc aagttcggcg acaagctccc cgcccagctg
     1921 cgggccgagc tcgacgggct caaggagcgc ctcggcgcct gacgcctgac gcctgacgcc
     1981 aggacccgat tcacggggcc ggttcctccc gggaccggcc ccgtggtcgt gacacgacgc
     2041 gcacacagcc cctcgtcctg gggcgggcgg cacgccatac tcgtgccgtg cctgccaacc
     2101 ctcgctacct gcccgcgccc gacaccgcag tcgtgcaccc cgactgggcc cgcaccgcct
     2161 cgatctacca gctcaacacg cgccacttca cgaccgaggg cacgttcgcg gccgcccgcg
     2221 cccacctgcc ccggctcaag gagctcgggg tgagcatcct gtggctcatg ccgatccacg
     2281 agatcgggcg caccaagcgc aagggcacgc tgggcagccc gtatgccgtc aaggactacc
     2341 tcagcgtcaa cccggagttc gggacggtcg aggacctcaa ggccttcgtc gacgccgcgc
     2401 acgagctcgg cctgcacgtc ctgctcgact gggtcgccaa ccacacggcc tgggacaacc
     2461 atctcgtcac cgaccacccg gagtggtacg cccgcgactg gaagggcgac ttctgcccga
     2521 cgccctggtg ggactgggac gacatcatcg acctcgacta ctcgcacccg gggctgcgtg
     2581 agtacatgac cgaggcgctg tgctactggg tccgcgaggt cggcatcgac ggataccgtt
     2641 gcgatgtggc cgggttcgta cccatcgact tctggaacac cgcccgcgcc acgctcgagg
     2701 agatcaagcc ggtcttcttg ctcgccgagt gggaggaccg cgacctgcac gtcggcgcgt
     2761 tcgacatgac ctacgcctgg tcgtggaaca tgacgatgca ccacatcgcc tcgggccggg
     2821 cggacgtcac cgacctgcgg gtcttctacg cctgggacgc caaggcctac cagcgcgaca
     2881 gcatccggat gatgttcgtc gccaaccacg acaagaacgc ctgggagggc accgagtccg
     2941 agcagttcgg cgagatgctc gactcggccg tggtcctgtc ggtggtcggc aagggcatgc
     3001 ccctggtctt caacggccag gaggccggca gtgacaaacg cttccagttc ttcgagaagg
     3061 acctcatcga ctggcgcgag cacccgatgg gcgacttcta ccggcgcctg ctcgtcctca
     3121 aacgcgacac gagcgccctg tggaacggcg cctggggcgc cccgatggtc aacgtcccca
     3181 acgacgccga gagcgccgtg ctgtcgttcg tgcgccagga cgaggccagc aaggtgttcg
     3241 cggtcttcaa cctctcgccg ctcgcccgcg gggtgtgctg ccgggagcac ctgtgccacg
     3301 ggacctaccg cgacttcttc tccggcaccg aggtcgtggt cggggacggc acgagcctcg
     3361 cgctgcccgc ctggggccac cggctgctgc tcgcctgagc ggccggccgg gccacgccca
     3421 tcgggtatgc cgggcccgcc gcctcccgct gggccttctc accccccgtg cttcaccggg
     3481 tgatctcacc ccctgctcag cgcgctgggc caccacatgc ggcggtcgac gatggcgacg
     3541 agcgccggca cgagcacggt cctgacgagc agcgtgtcga gcaggacgcc gaaccgacga
     3601 tgatcccgat ctgcccgagc gcgatgagcg ggagcacccc gagcacggtg aacaccgccg
     3661 caagcaggat cccggcgctg gtgatgacac caccggtcac cgccacggcg cgcacgatgc
     3721 ccgcggtcgt cccc
//
LOCUS       DATAIX010000714         3603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_259437, whole genome shotgun sequence.
ACCESSION   DATAIX010000714 DATAIX010000000
VERSION     DATAIX010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259437"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(653..1012)
                     /locus_tag="VHM65_10530"
     CDS             complement(653..1012)
                     /locus_tag="VHM65_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706185.1"
                     /translation="MVSLEYLRVVGEGSSPFGGGFPPVAQEVIAGVTSAVHARFFRGG
                     PDPANPEVPDGSIFLAALPTGVNQTGNGNVNVVEVMTTEDGGLAVLVNPLPTAPIDVG
                     LLRANQAPAVLDAFVDP"
     gene            complement(1006..1335)
                     /locus_tag="VHM65_10535"
     CDS             complement(1006..1335)
                     /locus_tag="VHM65_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706186.1"
                     /translation="MSERRRQHFASPCCGLADRRAPESSGQLVRGAFTASPDNVGLHA
                     PVEVVDVSGWSRGFWAASPRVTTFALHGFHDNGLWADTDFKILVGLTSGWPGPHRSPS
                     PPPSPSW"
     gene            complement(1346..3022)
                     /locus_tag="VHM65_10540"
     CDS             complement(1346..3022)
                     /locus_tag="VHM65_10540"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX2706187.1"
                     /translation="MSEVAISPSRSLPRQVGHETAHLHAVTPLEARRVAPVVRARPAE
                     PASMAELIADLANARERIRHGDPLGTARQHAKGRLTARERLEIVLDPGSFHELDLFAR
                     HQATGFGLENSRPETDGVVTGWGTVHGRRVMVYAHDARIFGGALGEVFAQKIQKVMDL
                     AEQAGIPVLGLGDGGGARIQEGVAALAGFGGIFRRNVRLSGVVPQISVVLGSCAGGAV
                     YSPALTDFVLMARDTSAMFITGPDVVSAVTGEDVSQQELGGANVHASESGVAALVGPD
                     ERSCLEDARFLLSLLPSNNTERPPHYEPTDSPERRCRTDIVPTRASAPYDVRDVITDV
                     VDDGEFLELHADWAANIVCGLARLDGHVVGIVGNQPLRLAGVLDVDASEKAGRFVRTC
                     DAFNIPLLTLVDVPGFLPGTEQERAGIIRHGAKLLYAYCEATVPRISVVLRKAYGGAY
                     IVMDSKSIGADLCFAWPASEVAVMGAEGAANIIFRKEIARAADPEGRRTELVQEYQDR
                     LMHSYTAAERGLVDDVIDPADTRRILIEAFALLRDKRTSAPTRKHGNIPL"
     gene            complement(3023..>3603)
                     /locus_tag="VHM65_10545"
     CDS             complement(3023..>3603)
                     /locus_tag="VHM65_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX2706188.1"
                     /translation="AVVADIGDFMLRRVEADELAGLDEPAARPAGETAAELAAPVDLR
                     GEVGIRPAEGAEAFVRALGSDFGPQVAVTALDLRTLLVKVRQLDRNRVDEDSPASHAD
                     GAEAPAGEYVAPSTELERTFIQLWEEAMGIKGIGIEHNFFELGGNSLVAAQLIARVRT
                     LMGIKLPMRTIFQSPTVAGMAASLEASAEAVS"
BASE COUNT          513 a         1272 c         1289 g          529 t
ORIGIN      
        1 ctacaaacac tccgtatcag atggtcccga accgactcca cccgaggcga ccctcggcga
       61 catcccccgg cggtgttcgt gcaggtcacg gacctacggt cgtctcccgt cgggccaggg
      121 cggttgttgt cggatcgtta ccaaccccgc ccgggcgggt cattgttggg cccattcctg
      181 ggcccaacac cgagtcagcg ggtcacggct gactcagccc ggatagcaac ccgatctatc
      241 cggacgccgg cgccgacgac gtcggggttc gcgtcactcg tcgtgccgcg gcctccagcc
      301 gggacgtgca gtcggggccg gttggcgttg cctgggtgtg ctcctcgcgc gggtcgccga
      361 cctgccggtg tacgaggcgt gaaggtcccg gacgcgttgc tcgccgtcga ccgcggcgcg
      421 tgaggccggc gatcagcgcc ggggccccgg gccgcgcaca ccggcctcga cgtgcgggac
      481 cctgtcgtcg acatctgggg gacgtcggct gctgcgacgc acgctgcgga cccgggtacg
      541 ccggcgatgt gccggacgag gtggtctgga aacgagtgtc cggcgccgca cctccggccg
      601 ctggctcgcg gtgcacaacc acgaagacgt cccagcgggc tgagccggcg ggtcacgggt
      661 cgacgaaggc gtccagaacg gccggcgcct ggttggcccg caggaggcct acgtcgatcg
      721 gtgcggtcgg gagcgggtta acgaggacgg cgaggccgcc gtcctcggtg gtcatgacct
      781 cgacgacgtt gacgttgccg ttgcccgtct ggttgacgcc ggtcgggagc gcggcgagga
      841 agatcgaccc gtccgggacc tctgggttag cgggatcggg accaccgcgg aagaaccgcg
      901 cgtggacggc cgaggtgaca ccggcgatga cctcctgcgc cacgggcggg aacccgccgc
      961 cgaacgggct gctcccctcg ccgaccacgc gcaggtactc caggctcacc atgagggact
     1021 tggtggcggg ctcggtgaac gatgcggccc aggccagccg gatgtcagcc cgacgaggat
     1081 cttgaagtcg gtgtccgccc agagtccgtt gtcgtggaag ccgtgcagcg cgaaggtcgt
     1141 cacgcgcggc gacgcggccc agaacccgcg cgaccacccg gagacgtcga cgacctcgac
     1201 gggcgcgtgc agccccacgt tgtccggcga ggcggtgaag gcccctcgga cgagctgccc
     1261 tgagctctct ggcgcacgac ggtcggccag gccgcaacac ggggacgcga agtgctgccg
     1321 ccgtcgttcg ctcatagctc gcctgtcaca gggggatgtt gccgtgcttg cgggtcgggg
     1381 cggacgtgcg cttgtcccgc agcagcgcga acgcctcgat caggatccgg cgggtgtcgg
     1441 cggggtcgat cacgtcgtcc accaggccgc gctcggcggc ggtgtagctg tgcatcagcc
     1501 ggtcctggta ctcctgcacg agttcggtac ggcgtccctc cgggtcggcg gcgcgggcga
     1561 tctccttgcg gaagatgatg ttggccgcgc cctcggcacc catcacggcg acctcactgg
     1621 ccggccaggc aaagcacagg tctgcgccga tcgacttgga gtccatgacg atgtacgcgc
     1681 cgccgtacgc cttgcgcagg acgacggaga tgcgcggcac ggtggcctcg cagtaggcgt
     1741 agagcagctt cgcgccgtgc ctgatgatgc ccgcgcgctc ctgctcggtg ccgggaagga
     1801 agcccgggac gtcgaccagc gtcagcagcg ggatgttgaa ggcgtcacag gtccggacga
     1861 accgtcccgc cttctccgag gcgtcgacgt cgaggacgcc ggccagtcgt agcggctggt
     1921 tgccgacgat gccgacgacg tgcccgtcga gtcgggccag cccgcagacg atgttggccg
     1981 cccagtccgc gtgcagctcc aggaactcgc cgtcgtcgac gacgtccgtg atgacgtccc
     2041 gcacgtcgta gggtgcgctc gcgcgggtgg gcacgatatc ggtgcggcac cgtcgctccg
     2101 gggaatcggt cggttcgtag tgcggcggcc gctcggtgtt gttggaaggc aacagcgaca
     2161 gcaggaaccg cgcgtcctcc aggcaggagc gctcgtccgg cccgacgagg gccgcgacgc
     2221 ccgactcgct cgcgtgcacg ttcgcccctc cgagctcctg ctggctcacg tcctccccgg
     2281 tcacggcgct cacgacgtca ggcccggtga tgaacatggc gctcgtgtcg cgcgccatca
     2341 ggacgaagtc ggtcagggcg ggcgagtaga ccgcgccgcc ggcacacgac ccgagcacca
     2401 cgctgatctg cggcacgaca ccggacagcc gcacgttgcg ccggaagatc ccgccgaagc
     2461 cggcgagggc ggccacgccc tcctggatcc gcgcgccacc accgtcgccc agcccgagca
     2521 ccgggatccc ggcctgttcg gcgaggtcca tcaccttctg gatcttctgc gcgaacacct
     2581 cgcccagcgc cccgccgaag atccgcgcgt cgtgcgcgta gaccatgacc cggcggccgt
     2641 gcacggtccc ccagccggtg acgacgccgt ccgtctccgg cctggagttc tccaggccga
     2701 agccggtcgc ctggtgccgg gcgaacaggt ccagctcgtg gaacgagccg ggatcgagga
     2761 cgatctccag ccgctccctg gccgtcagcc gccccttcgc atgctgccgg gcggtgccga
     2821 gcgggtcgcc gtgccggatc cgctcgcgcg cgttcgccag gtcggcgatc aactccgcca
     2881 tcgatgcggg ctcggccggg cgcgcccgca ccacgggggc cacgcgacgg gcttccagcg
     2941 gggtgacggc gtgcaggtgt gccgtctcgt ggccgacctg gcgtggcagc gaccggctcg
     3001 ggctgatggc cacttcgctc atctacgaca ctgcctcggc gctcgcctcg aggctcgctg
     3061 ccatgcccgc aacggtcggg gactggaaga tggtgcgcat cgggagcttg atgcccatca
     3121 gcgttcgcac gcgagcgatg agttgcgctg caacaagtga gttcccaccg agttcgaaga
     3181 agttgtgctc gatcccgatt cccttgatcc ccatcgcctc ctcccagagc tggatgaatg
     3241 tgcgctcgag ctcggtggag ggtgcgacgt actcaccggc cggtgcctcg gcgccgtcgg
     3301 cgtgcgacgc aggactgtcc tcgtcgaccc ggttccggtc gagctggcgg accttgacca
     3361 ggagggtacg aaggtcgagc gcggtgaccg cgacctgcgg gccgaagtcc gagccgagcg
     3421 cccgcacgaa ggcctcggcg ccctcggccg ggcggatgcc cacctcgccc cggagatcca
     3481 ccggcgcggc aagctccgca gcggtctcgc cggcaggccg cgcggccggc tcatcgaggc
     3541 cggcgagctc gtcggcctcc acccgccgga gcatgaagtc gccgatgtcg gcgacgaccg
     3601 cac
//
LOCUS       DATAIX010000715         2478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_140283, whole genome shotgun sequence.
ACCESSION   DATAIX010000715 DATAIX010000000
VERSION     DATAIX010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140283"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VHM65_10550"
     CDS             <1..889
                     /locus_tag="VHM65_10550"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2706189.1"
                     /translation="AADRAHPRLAQVSPMAPRRRLIVGGDGQLGRALRRIMPGADPID
                     RPDIDLTDPASVRSLPWADYDVVINAAAYTAVDLAESDAGRRDAWAVNAGAVATLAAV
                     ANEHRATLVHVSTDYVFDGTRETHTEDEPLSPLGVYGQSKAAGDIAATSCARSYVVRT
                     SWVVGDGPNFVRTMSDLADRGVSPSVVDDQFGRLTFADDLAAGIAHLIDTGAPYGTYN
                     LTNGGPVFSWADLAQAIFALRGRSATDVTRCSTQEFAAGRATAPRPRHSALDLAKVTA
                     TGWTPPDGMSRLAGYVAQL"
     gene            1045..2286
                     /gene="xseA"
                     /locus_tag="VHM65_10555"
     CDS             1045..2286
                     /gene="xseA"
                     /locus_tag="VHM65_10555"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX2706190.1"
                     /translation="MTGPSALPERALDTSADNPWPVRVLSLKIAGYVDKMSPLWVEGE
                     VVQLNRRDKTAYLVVRDPEVDMSLAVTISVFALDAVAVPLRVGARVVVQAKATFWPKR
                     GTLQLDARQIRPVGVGDLLARIEVLKARLSAEGLFDPARKKGLPFLPRTVGLITGRDS
                     AAERDVVENARRRWPSVRFAIRTVAVQGASAALDIATAVAELDADPAVDVIVIARGGG
                     GTEELLAFSNEALVRAVAAATTPVVSAIGHDIDTPLLDFVADWRASTPTDAGKRVVPD
                     AGDEGRLVQTSRERIRATLARRLEHERRRLVDVTTRPVMADRGAVLTSYREQVRALRR
                     RARQRQTSRVERAADEAGQLLLRVRALSPQSTLERGYAVVEQSDGRIVRDPRDLVPGE
                     PLHVRVARGDFAVRTEGIDDR"
BASE COUNT          352 a          944 c          853 g          329 t
ORIGIN      
        1 cgcggcggac cgggcacacc cacgtctggc gcaggtcagc ccgatggctc cccgacggcg
       61 gctcatcgtc ggcggcgatg gccagctcgg tcgggccttg cgccgcatca tgcccggcgc
      121 cgacccgatc gaccgccccg acatcgacct gaccgacccg gcctcggtcc ggtccctgcc
      181 ctgggccgac tacgacgtgg tcatcaacgc ggccgcctac acggcggtcg acctcgccga
      241 gagcgatgcg ggccgccggg acgcctgggc ggtcaacgcg ggcgccgtag ccaccttggc
      301 agccgtggcc aacgagcacc gtgcgacgct ggtccacgtc tcgaccgact acgtcttcga
      361 cgggacccgg gagacccaca ccgaggacga gccgctgtca cctctgggtg tctacgggca
      421 gagcaaggcg gcgggcgaca tcgcagccac ctcctgcgcc cgctcgtatg tcgtccggac
      481 cagctgggtg gtcggggatg ggcccaactt cgtccggacg atgtcggacc tcgcagaccg
      541 cggcgtcagc ccgtcggtcg tcgacgacca gttcggccgg ctgaccttcg ccgacgacct
      601 cgccgcgggc atcgcccacc tcatcgacac cggcgcacca tacggcacct acaacctcac
      661 caacggcgga cccgtgttct cctgggcaga cctggcccag gccatcttcg ccctgcgtgg
      721 ccggtcggcc accgacgtca cgcggtgctc gacgcaggag ttcgcggcgg ggcgagccac
      781 cgccccccgg cctcgccaca gcgccctgga cctggccaag gtcaccgcca ccggatggac
      841 acccccggac ggtatgtccc gcctggccgg ttacgtcgcc cagctgtagc ccatcccgct
      901 cggtgccggt cgcgcgccac acagtgttga tcgtgacaca gtgctgaccg tgactcagtg
      961 ctgaccgtga ctcagtgctg accgtgactc agtgctgacc gtgactgagt gctcccgtga
     1021 cgctgaccgt gacacagtga ggccgtgacc ggaccgtccg cgctgcccga gcgcgcgctc
     1081 gacacgagcg cggacaaccc gtggccggtg cgggtgctca gcctgaagat cgccggctac
     1141 gtcgacaaga tgtccccgct ctgggtcgag ggggaggtcg tccagctcaa ccggcgcgac
     1201 aagaccgcct acctcgtcgt gcgcgacccc gaggtcgaca tgagcctggc cgtgaccatc
     1261 tcggtcttcg ccctcgacgc cgtggccgtc ccgctacggg tcggcgcccg ggtggtcgtg
     1321 caggccaagg caaccttctg gcccaagcgc ggcacgctcc agctcgacgc gcgccagatc
     1381 cgcccggtcg gcgtgggcga cctgctcgcc cggatcgagg tcctcaaggc gcgcctgtcg
     1441 gccgagggcc tgttcgaccc ggcccgcaag aagggcctgc ccttcctccc ccgcaccgta
     1501 gggctcatca ccgggcgcga cagcgccgcc gaacgggacg tcgtcgagaa cgcccgccgc
     1561 cgatggccca gcgtccggtt cgcgatccgc acggtcgccg tgcagggggc atcggctgcg
     1621 ctcgatatcg ccacggccgt ggccgagctc gacgccgacc ccgccgtcga tgtcatcgtc
     1681 atcgcccgcg gaggtggggg cacggaggag ctcctggcgt tcagcaacga ggcgctggtc
     1741 cgggccgtgg cagcggccac cacgccggtc gtcagcgcga tcgggcacga catcgacacg
     1801 ccgctgctcg acttcgtcgc cgactggcgc gcctcgacgc cgaccgacgc cggcaagcgt
     1861 gtggtgccgg atgccggcga cgagggccgg ctcgtgcaga cctcacgcga gcggatccgg
     1921 gccacgctgg cccggcggct cgagcacgag cgccgccggc tcgtcgacgt caccacccgc
     1981 ccggtcatgg ccgaccgcgg ggccgtgctg acgtcatacc gcgagcaggt ccgtgccttg
     2041 cgccgtcggg cccggcagcg ccagacgagc cgggtcgagc gagccgccga cgaggccggc
     2101 cagctcctcc tgcgcgtgag ggcgctgtcc ccccagtcaa cgctggagcg gggatacgcc
     2161 gtcgtcgagc agtccgatgg gcgcatcgtg cgcgaccccc gcgacctcgt tcccggtgag
     2221 ccgctgcacg tccgggtggc ccggggcgac ttcgccgtcc gcacggaggg catcgacgac
     2281 aggtagggtc agatcgcccc ggctcgtgcg ggccccagga caaggagagt cacgtatggc
     2341 gggacagtca ccgccgccgg ccacggcggc cgcaccggcc acaccggagt ttgccgacat
     2401 tgccggcctg ggctacgaac aggcccggga cgagctcgtc gagatcgtgg ccaggctcga
     2461 gggcggccag gcacccct
//
LOCUS       DATAIX010000716         4899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_207897, whole genome shotgun sequence.
ACCESSION   DATAIX010000716 DATAIX010000000
VERSION     DATAIX010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207897"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(307..570)
                     /locus_tag="VHM65_10560"
     CDS             complement(307..570)
                     /locus_tag="VHM65_10560"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX2706191.1"
                     /translation="MRYGLRLSAAARSALSETLPGQIAAAVWEFIDGPLRDNPHRVGK
                     PLRLRLEGFHSARRGQYRVIYRILDTEVIVEMVKISHRSDTYG"
     gene            complement(567..848)
                     /locus_tag="VHM65_10565"
     CDS             complement(567..848)
                     /locus_tag="VHM65_10565"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX2706192.1"
                     /translation="METTSLADAKNRLSELVNAVQGTWERVTITKNGRPAAVLIAPDD
                     LESLIETLQILSDPEAMAAIGEAVAGTGEMTTGEEMAAIMARRPGRGAA"
     gene            complement(992..3127)
                     /locus_tag="VHM65_10570"
     CDS             complement(992..3127)
                     /locus_tag="VHM65_10570"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2706193.1"
                     /translation="MTERQPGRARDPDLSGVTERDGVRLAYDVFGEGETTVLLMPTWS
                     IVPSRFWKAQIGYLARHYRVVTFDGRGSGRSGRPLGAAAYTNEQYADDTLAVMDATGT
                     REAVLVALSCGATWAVHAAAEHPERVLGIFAMSPACGFAIPQPDRDHYPWQGRSASTD
                     GWGKYNKHYWLEGGYEDFLAFFFEKMFTEPHSTKQIEDCVGWGREIAPETLADTTAGR
                     LGCDGAVCMPLEPVCAQVRCPVTVVHGTDDAVRPHQVGERLAELTGGSLVLLDGAGHG
                     PMTRHPVRINLMIREFVDRLRPPVERHTWVAAPRRRRRALYLCSPIGLGHARRDLAVV
                     DELRQLHPDLEVDWLAQHPVTTVLEAAGERIHPASAWLANESAHIEHEAGEHDLHAFQ
                     AIRRMDEILVSNFMVFQEVVEQEHFDLVVGDESWEVDYFLHENPDLKRFAFAWLTDFV
                     GWLPMPDGGEREAELTADYNAEMIEQRARFARVRDRSVFVGNPEDVVPQRFGPGLPMI
                     SDWVRDNFDFAGYITGFDTAGLDNRDALRQRLGYRDDERLCLVTVGGSAVGAPLLRRV
                     LDAAPLVRRALPDLRFVIVAGPRIDPGSLPRRRGVSILGHVPDLYQHLAAADLAVVQG
                     GLTTTMELTASQRPFVYVPLRHHFEQNIHVRHRLDRYGAGRCLDYEEAVDPDALADAI
                     LKEVDHKVAYRPVETDGAAVAARMLAELL"
     gene            complement(3124..4203)
                     /locus_tag="VHM65_10575"
     CDS             complement(3124..4203)
                     /locus_tag="VHM65_10575"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2706194.1"
                     /translation="MTQLLQPAEVDFDKLMAFVFRSVEEIGATLNAGLVVMGDKLGYY
                     RAMAASGPTTPSRLADDTGTAEPYAREWLNAQAAGGIVEYDPISGDYTLPPEHVVAFT
                     DESSPAFLPGFFQIALGTVQDTSKIIELARSGAGFGWHEHNHDVHEGCERFFRPSYNA
                     HLVDEWLPALDGVVAKLEYGARVADIGCGYGASTIIMAQAFPKSTFVGSDYHGDSIEQ
                     ARARAEAAGVGERVRFEVAPAHSFSGTGYDLVTTFDALHDMGDPVGAARQVRRNVAPD
                     GTWLVVEPMAGDRVQDNLNPVGRAYYGFSTLLCTPASLSQEVGLALGTQAGPARIRDV
                     VTTGGFTRFAKVAETPFNQVLEVRP"
     gene            4356..>4899
                     /locus_tag="VHM65_10580"
     CDS             4356..>4899
                     /locus_tag="VHM65_10580"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2706195.1"
                     /translation="MLVGRESERRTISTLLAGARVGDSRVLVLSGEPGIGKTALLTEA
                     ETLVGDMRVLRARGVESEQFVPFAGLLQLLRPVLTLLEHIPAPQSRALSSALLLGSGR
                     EVDPSRFAVGAATLSLLCRAAEDRPLAVLVDDAHLLDSPSAGALVFAARRLVTDAVAI
                     LVSVRAEEPGTAAWGSLPTLT"
BASE COUNT          692 a         1735 c         1740 g          732 t
ORIGIN      
        1 gcgtggacga tggcgacgac cagaaatgcg atcactgcga ggaccgcgag gctccatccg
       61 gtgctcatca acgccaacaa cgcctgggtc gggtcaccgt cgcgctgcgc cgtttcgggt
      121 ctgctcgaca tgggagccgc cacgaccggg ccggccacga ccactaccag caacgcgcac
      181 gcgggaactg cggaagcggg cggcataccg gcgggtcttg gcgagcatcc ggtattggcg
      241 ccagccccgg ccagcctcaa tgcggcgcgt gtcgtcgcct ccttcgagcg ccgggtccgt
      301 tcacgactat ccgtaggtgt cactgcggtg ggagatcttg accatctcga cgatgacctc
      361 ggtgtcgagg atccggtaga tcacccggta ctggcctcgc cgggcggagt ggaagccctc
      421 cagccgcagg cgcagaggct tgccgacccg atgagggttg tcccgcaacg gcccgtcgat
      481 gaactcccac acggcagcgg cgatctgtcc gggcagggtc tcggacagcg cgctccgggc
      541 cgccgcggag aggcgcagtc cgtacctcac gcagcgccgc ggcccgggcg ccgcgccatg
      601 atcgcggcca tctcctcacc cgtggtcatc tccccggtcc cggccacggc ctccccgatc
      661 gcggccatcg cctccgggtc ggagaggatc tgcagggtct cgatcaggct ctccagatcg
      721 tcgggggcga taagaaccgc cgccggccgc ccgttcttgg tgatggtgac tcgctcccag
      781 gtgccctgga cggcgttgac cagctcactg agccggttct tcgcatcggc caatgacgtc
      841 gtctccatag ccagaattct agccacatcg acgggtcacg tcacccgtgc gcgccgacaa
      901 accgtgcgcg ccgacatacc gtgtcagcgc cagcgcaccc agcggcaccg cagtcacttg
      961 cccgctgctg gcacctgccc gaccggagac gtcagaggag ctcggccagc atgcgtgcgg
     1021 cgaccgcagc accgtcggtc tcgaccggcc ggtaggccac cttgtggtcg acctccttga
     1081 ggatggcgtc ggcgagcgcg tcggggtcga cggcctcctc gtagtcgagg caccgtccgg
     1141 cgccataccg gtcgaggcgg tgccggacgt ggatgttctg ctcgaaatgg tgccgcagcg
     1201 ggacgtagac gaacggccgc tggctggcgg tgagctccat ggtggtggtg agcccgccct
     1261 ggacgacggc gaggtcggcg gcggccaggt gctggtagag gtccggcacg tggccgagga
     1321 tcgacacacc ccggcgtcgc ggcagggacc ccgggtcgat ccgcgggccg gccacgatca
     1381 cgaagcgcag gtcgggcagc gctctgcgca ccaggggtgc cgcgtcgagg acccggcgca
     1441 gcaacggcgc accgaccgcg gagccgccca cagtgaccag gcagagccgc tcgtcgtcgc
     1501 ggtagcccag ccgctgccgc agggcgtccc ggttgtcgag gccggcggtg tcgaagccgg
     1561 tgatgtagcc ggcgaagtcg aagttgtccc ggacccagtc gctgatcatc gggagcccgg
     1621 gaccgaacct ctgcggcacg acgtcctcgg ggttgccgac gaacaccgac cggtcccgga
     1681 cccgggcgaa gcgggcccgc tgctcgatca tctccgcgtt gtagtcggcg gtcagctccg
     1741 cctcgcgctc cccgccgtcg ggcatcggca gccagccgac gaagtcggtc aaccaggcga
     1801 aggcgaagcg cttcaggtcc gggttctcgt gcaggaagta gtccacctcc caggactcgt
     1861 ccccgacgac gaggtcgaag tgctcctgct cgacgacctc ctggaacacc atgaagttgc
     1921 tgacgaggat ctcgtccatc cgccggatcg cctggaaggc gtgcaggtcg tgctccccgg
     1981 cctcgtgctc gatgtgcgcg gactcgttcg ccagccaggc cgaggccggg tggatgcgct
     2041 ccccggcggc ctcgagaacg gtcgtgacgg ggtgctgggc gagccagtcg acctcgaggt
     2101 ccgggtgcag ctgccgcagc tcgtcgacga cggcaaggtc gcgcctggca tggccgagcc
     2161 cgatgggcga gcagaggtac aacgcgcgcc ggcgacgccg cggggcggcg acccaggtat
     2221 gccgctcgac cggcgggcgg agccggtcga cgaactcgcg gatcatcagg ttgatccgca
     2281 ccgggtgccg cgtcatcggg ccgtgtcccg ccccgtcgag cagcaccagc gatcccccgg
     2341 tcagctcagc cagccgctct ccgacctggt gcgggcggac cgcgtcgtcg gtgccgtgga
     2401 cgaccgtcac cgggcaccgg acctgagcgc agaccggctc cagcggcata cacaccgccc
     2461 cgtcgcagcc gagccggccg gcggtggtgt cggcgagggt ctcgggggcg atctcgcggc
     2521 cccagccgac gcagtcctcg atctgcttgg tcgagtgcgg ctcggtgaac atcttctcga
     2581 agaagaaggc caggaagtcc tcgtagccgc cctcgagcca gtagtgcttg ttgtacttgc
     2641 cccacccgtc ggtgctcgca ctgcgaccct gccaggggta gtggtcccga tccggctggg
     2701 ggatggcaaa cccgcacgcg ggcgacatcg cgaagatgcc caggacccgc tccgggtgct
     2761 ccgcggccgc gtgcaccgcc caggtggcgc cgcaggagag cgcgacgagc accgcctcgc
     2821 gggttccggt ggcgtccatg accgcgaggg tgtcgtcggc gtactgctcg ttggtgtatg
     2881 ccgccgcccc gagcggccgg ccggaccggc cgctgccccg cccgtcgaag gtgacgacgc
     2941 ggtagtgacg ggccaggtag ccgatctggg ccttccagaa ccgggagggc acgatgctcc
     3001 acgtcggcat cagcagcacc gtcgtctcgc cctcgccgaa cacgtcgtag gcgaggcgca
     3061 caccgtcgcg ctcggtgacc ccgctgaggt cggggtcccg ggcccgaccg ggttgccgtt
     3121 cggtcacggc ctgacctcca gcacctggtt gaagggtgtc tcggcaacct tggcgaagcg
     3181 ggtgaacccg cccgtggtga ccacgtcccg gatgcgggcg gggccggcct gggtgccgag
     3241 ggccagtccg acctcctgcg agagcgaggc gggagtgcac agcagtgtgg agaacccata
     3301 gtaggcccgc cccaccgggt tgaggttgtc ctggacccgg tcaccggcca tcggctccac
     3361 caccagccag gtgccgtcgg gggcgacgtt gcggcgcacc tgccgggccg ccccgaccgg
     3421 gtcccccatg tcgtgcagcg cgtcgaacgt ggtgacgagg tcgtagccgg tcccgctgaa
     3481 cgagtgggcg ggcgccacct cgaagcgcac ccgctcgccg accccggccg cctcggcccg
     3541 ggcccgggcc tgctcgatcg agtcgccgtg gtagtccgat cccacgaagg tggacttcgg
     3601 gaaggcctgc gccatgatga tcgtcgacgc accgtacccg cacccgatgt cggccacccg
     3661 ggcgccatac tcgagcttgg cgaccactcc gtcgagggcg ggcagccact cgtcgacgag
     3721 gtgcgcgttg tagctgggcc ggaagaaccg ctcgcagccc tcgtgcacgt catggttgtg
     3781 ctcgtgccag ccgaagcccg caccgcttcg ggccagctcg atgatcttcg acgtgtcctg
     3841 gaccgtgccg agcgcgatct ggaagaaccc gggcaggaag gcggggctgg actcgtcggt
     3901 gaaggcgacg acatgctcgg gcggcagcgt gtaatccccc gagatcgggt cgtactcgac
     3961 gatcccgccg gctgcctggg cgttgagcca ctcccgggcg tagggctcgg cggtgccggt
     4021 gtcgtcggcg agacggctcg gcgtcgtcgg cccggaggcg gccatggccc ggtagtagcc
     4081 gagcttgtcc cccatgacga cgagcccggc gttcagggtg gccccgatct cctcgacgga
     4141 ccggaagacg aaggccatca gcttgtcgaa gtccacctct gcgggctgga gcagctgggt
     4201 cattgtcgtt gtcctctctc gttgttcctc gatctcagtt gttcctcgat gtaggcccga
     4261 ccgtaggtcc gccctcgccg cggtcgcatc aggtgcccca cctagtcctt ccggccgcca
     4321 gggcagcccc ggccaggggg catagactcc gagacgtgct ggtcgggcgg gagtctgagc
     4381 ggcggaccat cagcacgctc ctggccggcg cgagggtggg cgacagccgt gttctcgtgc
     4441 tgagcggtga gcccggcatc ggcaagaccg cgctcctcac cgaggccgag acgctcgtcg
     4501 gggacatgcg ggtgctgcgg gcccggggcg tcgagtccga gcagttcgtc cccttcgccg
     4561 gtctgctcca gctgctccgc ccggtgctca cgttgctcga gcacatcccg gcgccccagt
     4621 ccagggccct gtcctcggca ctcctgctcg gttcgggccg cgaggtcgac ccgagccgtt
     4681 tcgcggtggg ggcggcgacc ctgagcctgc tgtgccgggc cgccgaggac cggccgctgg
     4741 ccgtgctggt cgacgacgcg cacctgctcg acagcccgtc ggcgggggcg ctcgtcttcg
     4801 ccgcccggcg gctggtcacc gacgcggtcg ccatcctggt cagtgtccgc gccgaggagc
     4861 ccgggacggc ggcctggggc tcgctgccca cccttaccc
//
LOCUS       DATAIX010000717         1776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_238295, whole genome shotgun sequence.
ACCESSION   DATAIX010000717 DATAIX010000000
VERSION     DATAIX010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238295"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VHM65_10585"
     CDS             complement(<1..561)
                     /locus_tag="VHM65_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706196.1"
                     /translation="MDEAVTVSMVETSAEAHEAAALLGRIWSSPRPIVPADLLFALVR
                     VRNYAALARVGPEVVGASVAIRGVSGGGSPLLHSHVTGTTRPGVGRILKEHQRQWALA
                     EGLPTITWTVDPLLRRNAVFNLDVLGARVDGYRVNHYGSMTDGRNSGEESDRFCVVWD
                     LDPASPAGALPAGEAPTMGRPARPSPE"
     gene            complement(643..1131)
                     /locus_tag="VHM65_10590"
     CDS             complement(643..1131)
                     /locus_tag="VHM65_10590"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX2706197.1"
                     /translation="MPSPVAVGAAAGLDRLDRSILRLLQEDGRLSNVDLARRVGLSPS
                     PCLRRVKALEQRGYIQRYAAVLSRALLGRDLVVDVLVNLTDQRQETLAGFEAAVCEID
                     DILRCSLIAGDADYLLTVAVENLDAYQRLFSRRLGELPGVASLKSLVTMKAVKDSRSL
                     PL"
     gene            1307..>1776
                     /locus_tag="VHM65_10595"
     CDS             1307..>1776
                     /locus_tag="VHM65_10595"
                     /inference="COORDINATES: protein motif:HMM:NF017072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706198.1"
                     /translation="MKIGIPREVKNNEFRVAITPVGVHELVNQGHQVLVQKDAGVGSS
                     ISDGEYVAQGASILGSADEVWGQAEMVMKVKEPVAEEYHLLREDLVLFTYLHLAADKT
                     QTEALLAAGTTAIAYETVQLPSGMLPLLYPMSEVAGCLAPQVGAHALMKAQGGRG"
BASE COUNT          282 a          597 c          633 g          264 t
ORIGIN      
        1 ctcgggtgac ggccgggccg gccggcccat ggtgggcgcc tctcccgccg ggagcgcccc
       61 ggcgggcgaa gcggggtcga gatcccacac gacgcagaat cggtcggact cctcgccgct
      121 gttgcgcccg tcggtcatgg acccgtagtg gttgacccgg tagccgtcga cccgggcacc
      181 gaggacgtcg aggttgaaga cggcgttgcg cctcagcagc gggtcgaccg tccacgtgat
      241 cgtgggcagg ccctcggcca gagcccactg ccgctgatgc tccttgagga tccggccgac
      301 acccggtctg gtcgttcccg tcacgtgcga gtgcagcagc gggctaccgc cacccgacac
      361 accccggatg gccacactcg ccccgacaac ctccgggccg acccgggcca gcgccgcgta
      421 gttgcgtacg cggaccagcg cgaagagcag gtctgcgggg acgatcgggc gcggcgacga
      481 ccagatgcgg ccgagcaggg cggcggcttc gtgcgcctcg gcgctcgtct cgaccatcga
      541 caccgtgacg gcctcgtcca tggcactcct gctgctcgat ctgcggcgcc cgcccgagac
      601 gcgccctacc acgataggcg gaccagcgcc cccgctgccc tctcagagcg gcaggctcct
      661 cgagtccttg accgccttca tcgtcacgag gctcttgagc gaggcgacgc cggggagctc
      721 gccgagtcgc ctgctgaaga gccgctggta ggcgtcgagg ttctccaccg cgacggtgag
      781 caggtagtcg gcgtcgccgg cgatgagcga gcagcgcagg atgtcgtcga tctcgcagac
      841 ggcggcctcg aagccggcca gggtctcctg ccgctggtcg gtgaggttga cgaggacgtc
      901 gaccaccagg tcacggccga gcagcgcgcg gctgagcacc gcggcatacc gctggatgta
      961 cccgcgctgc tccagggcct tgacccggcg cagacacggc gagggcgaga gcccgacccg
     1021 cctggccagg tcgacgttgc tgagccggcc gtcctcctgc agcaggcgca ggatcgaccg
     1081 gtcgaggcga tccagacctg cggccgcacc gaccgccacc ggtgatggca tgaaacactc
     1141 ccatagttcg actgtcgggc atcatcatgc gccgatctcg gtgaacgacg acaattatgg
     1201 caccatctgc cggtcattcg gcactagact tgccgaccgg aggagttacc cggtccggtc
     1261 gatcgaccgg ccggtcgtgg cagttgagtc caggagacgg tgagccgtga agatcggaat
     1321 ccctcgcgag gtcaagaaca acgagttccg cgtcgcgatc actccggtgg gggtgcacga
     1381 gctggtgaac cagggtcatc aggtgctggt gcagaaggac gccggtgtgg ggtcctcgat
     1441 cagcgacggg gagtatgtcg ctcagggcgc gtcgatcctc ggctcggccg atgaggtgtg
     1501 gggccaggcc gagatggtga tgaaggtcaa ggagccggtg gccgaggagt accacctgct
     1561 gcgcgaggac ctggtgctgt tcacctacct gcacctggct gccgacaaga cccagaccga
     1621 ggcgctgctg gcggcgggga cgaccgcgat cgcctacgag acggtgcagc tgccttcggg
     1681 gatgctcccg ctgctctacc ccatgtcgga agtggccgga tgtctggctc cccaggtcgg
     1741 cgcgcatgcg ctgatgaagg cccagggtgg ccgggg
//
LOCUS       DATAIX010000718         2278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_140533, whole genome shotgun sequence.
ACCESSION   DATAIX010000718 DATAIX010000000
VERSION     DATAIX010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140533"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VHM65_10600"
     CDS             complement(<1..213)
                     /locus_tag="VHM65_10600"
                     /inference="COORDINATES: protein motif:HMM:NF018672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEX2706199.1"
                     /translation="MRPLATVLVVLVAALHAYFAVLEMALWTTPAGRTAFGLGLEFAV
                     ASKSLAANQGLYNLFLVAGLVWGLVAG"
     gene            431..1954
                     /gene="hutH"
                     /locus_tag="VHM65_10605"
     CDS             431..1954
                     /gene="hutH"
                     /locus_tag="VHM65_10605"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX2706200.1"
                     /translation="MGVGPVSHGDVVAVARREARVELSPDAVAAIQASRAVITALATD
                     SAPHYGVSTGFGALATRHIPAPLRAQLQRSLVRSHAAGSGPEVEREVVRALMLLRLST
                     LATGRTGVRVETARAYAAMLSAGVTPVVYEYGSLGCSGDLAPLAHCALAAMGEGFART
                     DDGGLVPAREALEAAGIAPVVLEEKEGLALINGTDGMLGMLVLAIEDLRLLLRTADVT
                     AAMSVEGLLGTDHVFAADLHDLRPHPGQGTSAANLRKLLAHSPIRASHDTEDCPRVQD
                     AYSMRCAPQVHGAARDTVEYAAQVASRELASAIDNPVVTLDGRVESNGNFHGAPIGYV
                     LDFLAIVAADVASMSERRTDRFLDTNRNHGLNAFLADDPGVDSGHMIAQYTQAAIVSE
                     LKRLAAPASVDSIPSSAMQEDHVSMGWSAARKLRRSVDGLARVLGIELLTAARGIDLR
                     APLQPGPATASVVAGLRETTPGPGTDRYLAPEIESAVTFVARGQALAAAESVTGPLA"
     gene            2035..>2278
                     /locus_tag="VHM65_10610"
     CDS             2035..>2278
                     /locus_tag="VHM65_10610"
                     /inference="COORDINATES: protein motif:HMM:NF036634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706201.1"
                     /translation="MEGARPVRAHRGTTLTAGHWGAEAPLRMLQNNLDPEVAERPDDL
                     VVYGGTGRAARDWRSFDAMVRTLTTLKPDETMLVQSG"
BASE COUNT          322 a          899 c          773 g          284 t
ORIGIN      
        1 gcccgcgacc agcccccaga cgagcccggc gacgaggaac aggttgtaga ggccctggtt
       61 ggcggcgagt gacttgctgg ccaccgcgaa ctcgaggccg aggccgaagg cggtccgtcc
      121 ggcgggcgtg gtccacaggg ccatttccag cacagcgaag taggcgtgca gtgcggcgac
      181 gaggaccacg aggacggtgg cgagagggcg catggcgccc agcatgtcag ggcccggccc
      241 gagttgtctc ggatccgaga cgcaatcgcc ggtgcagaga tggcaggtgc gcggcgctgc
      301 cgctccaatg ggaggcatga gtgatgcgcg agtggcaggc ccgtggccat ccgatggggc
      361 agatgtgcca ggtacgccgg agaagccggg gtctgagcac cccgagcctg ccgcccggac
      421 caccgttgtc gtgggtgtcg gaccggtcag ccacggtgac gtcgtcgccg tggcgcgccg
      481 cgaggcccgg gtcgagctca gccccgacgc cgtcgccgcg atccaggcca gccgggccgt
      541 catcacggcg ctcgccacgg actcggcgcc ccactacggc gtctcgaccg gcttcggtgc
      601 gctcgcgacc cggcacatcc ccgccccgct gcgcgcccag ctgcagcgca gcctcgtccg
      661 ctcgcacgcg gccggctcag gccccgaggt cgagcgcgag gtggtccggg ctctcatgct
      721 cctgcgcctg tcgaccctcg cgaccggccg caccggggtc cgggtcgaga cggcccgcgc
      781 ctacgccgcc atgctctcgg ccggcgtcac cccggtcgtg tacgagtacg gctcgctcgg
      841 ctgctccggc gacctcgcgc ccctggccca ctgcgcgctc gcggcgatgg gggaaggctt
      901 cgcccgcacc gacgacggcg gcctcgtccc ggcccgggag gccctcgagg ccgcgggcat
      961 cgcgccggtc gtcctcgagg agaaggaggg cctcgccctc atcaacggca ccgacggcat
     1021 gctcgggatg ctcgtcctcg ccatcgagga cctgcggctg ctgctgcgca cggccgacgt
     1081 caccgccgcg atgagcgtcg agggcctgct cgggaccgac cacgtcttcg ctgcggacct
     1141 gcacgacctg cgcccccatc ccggccaggg cacctcggcc gccaacctgc gaaagctgct
     1201 cgcccacagc ccgatccgcg ccagccacga cacggaggac tgcccccggg tccaggacgc
     1261 ctactccatg cgctgcgcgc cgcaggtgca cggggcggct cgggacaccg tggagtatgc
     1321 cgcgcaggtc gcctcccgcg agctcgcgtc ggcgatcgac aacccggtcg tcaccctcga
     1381 cgggcgagtc gagtccaacg gcaacttcca cggcgcgccg attggctacg tgctcgactt
     1441 cctggccatc gtcgccgccg atgtcgcctc gatgtccgag cgccgcaccg accgcttcct
     1501 ggacaccaac cgcaaccacg ggctcaacgc cttcctcgcc gacgaccccg gggtcgactc
     1561 cggccacatg atcgcccagt acacccaggc cgcgatcgtc tccgagctca agcggctggc
     1621 cgcccccgcc agcgtcgact cgatcccgag cagcgcgatg caggaggacc acgtctcgat
     1681 ggggtggtcg gcggcccgca agctgcgccg cagcgtcgac gggctggccc gcgtgctggg
     1741 gatcgagctg ctcaccgccg cccggggcat cgacctgcgg gcccccctgc agccgggccc
     1801 ggccaccgcc tcggtggtcg ccgggctgcg cgagacgacc cccggtccgg gcaccgaccg
     1861 ctacctcgcc ccggagatcg agtccgccgt caccttcgtc gcccgtgggc aggccctggc
     1921 ggcggccgag tcggtcaccg gcccgctcgc ctagccagcc agacacccag ccggcatacc
     1981 ccccccggcc tacccagccg tcatacgcca gcactgactc taaggagacc acccatggaa
     2041 ggtgctcgtc ccgtccgcgc ccaccgcggc acgaccctca ccgcaggcca ctggggcgcc
     2101 gaggccccgc tgcggatgct ccagaacaac ctcgaccccg aggtcgccga acgccccgac
     2161 gacctcgtcg tgtatggcgg gaccggccgg gccgcgcgcg actggcgctc cttcgacgcc
     2221 atggtccgga ccctgacgac gctcaaaccg gacgagacga tgctcgtcca gtccggac
//
LOCUS       DATAIX010000719         2499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_208488, whole genome shotgun sequence.
ACCESSION   DATAIX010000719 DATAIX010000000
VERSION     DATAIX010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208488"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VHM65_10615"
     CDS             <1..235
                     /locus_tag="VHM65_10615"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706202.1"
                     /translation="PRVNPYGGAIALGHPLAASGVRLMIQLARHFQDHPEVRFGVTAM
                     CVGIGMGASVIWENPHHADYVSSATAPATEDVA"
     gene            280..2400
                     /locus_tag="VHM65_10620"
     CDS             280..2400
                     /locus_tag="VHM65_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX2706203.1"
                     /translation="MTSGTDLAAMFPDEVVTKCPVRDVALPGGLGTLALITLDNGRDH
                     TRPNTFGPSSMAALDAALDALRTRAEAGEIVAVAVTGKPFIFAVGADLSGFPMVTRRE
                     LALAFAAGGHATFAKLSDLPVPSFAFVNGAAMGGGVELGLAATYRTISAGVPALALPE
                     CFLGLIPGWGGCYLLPNLVGPKAALTVIVENAMSNNKMLKGPAAFELGIADAIFEPAD
                     FLEESIGWAARVLSGQVEVDRTPVDRDEAAWNGAVAATRAIVDAKTGGTAPAPYRALD
                     LVTAARTARRDEAFAAEDEAIADLVMSDELRAGLYAFDLVQKRAKRPAGAPDRGLARP
                     VTKVGIVGAGLMASQLALLFAQRLEVPVVLTDLDEQRVAKGVGYVHNELDKLAGKGRL
                     SSDKASRYKSLVSGSTSKSGFSDADFVIEAVFEEMGVKKQVFAEVEAVVPETCVLATN
                     TSSLSITEMASGLAYPGRVVGFHFFNPVAVMPLLEIITGEQTDDATLATAFATGKSLR
                     KTCVLVKDSPSFIVNRLLGRFMGEVGRVVDNGTPVAVADQAFTGLAPMPPFVLMGLVG
                     PAIALHNSETLARAFPGRFYVAEGLRRMVEAKKSSFYTFAGGEMVVDPQAAALMPSPP
                     QPVVLTAAEVRDQVLSGLADEARRMLDEGVVQAPMDLDLALITGAGFAFNNGGLTPLL
                     DRSGVAERVTGRRFLPPGVASVPA"
BASE COUNT          361 a          906 c          855 g          377 t
ORIGIN      
        1 cccgagagtc aacccgtatg gcggcgccat tgcgctcggt catcccctcg ccgcgtccgg
       61 ggtgcgactc atgatccagc tggcacgcca cttccaggac caccccgagg tccgtttcgg
      121 cgtcacggcg atgtgcgtcg gcatcgggat gggtgccagc gtcatctggg agaaccctca
      181 ccacgctgac tacgtgagca gcgcaaccgc accggcgacc gaggacgtgg cctgatgaca
      241 accggcacca cgacacctga gagcgcgaca cctgacagca tgacgagcgg gaccgacctc
      301 gcggcgatgt tcccggacga ggtcgtcacg aagtgcccgg tccgcgacgt cgcccttccc
      361 ggcgggctcg gcaccctggc cctcatcacg ctggacaacg ggcgtgacca cacccggccg
      421 aacaccttcg gccccagctc catggcggcg ctggacgcgg cgctcgacgc gctgcgaacc
      481 cgggccgagg ccggcgagat cgtcgccgtc gccgtgacgg gcaagccgtt catcttcgcg
      541 gtcggcgcag acctgagcgg gttccccatg gtcacccggc gcgagctggc actggcgttt
      601 gccgccgggg gccatgccac gttcgccaag ctgagcgatc tcccagtgcc ctcgttcgcg
      661 ttcgtcaacg gagcggcgat gggcggcggg gtcgagctcg gcctcgctgc gacataccgg
      721 acgatctcgg ccggcgtgcc cgcgctggcc ctgccggagt gcttcctcgg cctcatcccc
      781 ggctggggcg gctgctacct gctccccaac ctggtcgggc ccaaggcggc gctcacggtc
      841 atcgtggaga acgcgatgtc caacaacaag atgctcaagg gcccggcggc gttcgagctc
      901 ggcatcgccg atgccatctt cgagccggcc gacttcctcg aggagtcgat cggctgggcg
      961 gcgagggtgc tcagcggcca ggtcgaggtc gaccgcacgc cggtggaccg ggacgaggcc
     1021 gcctggaatg gcgccgtcgc ggccacccgg gccatcgtcg acgccaagac cggcgggacg
     1081 gctcctgcgc cataccgggc cctcgacctc gtcacggcgg cgcggaccgc ccgccgggac
     1141 gaggccttcg ctgcggagga cgaggccatc gccgacctgg tcatgagcga cgagctgcgc
     1201 gccgggctct acgccttcga cctcgtccag aagcgggcca agcgccccgc tggggctccc
     1261 gaccgggggc tcgcgcggcc cgtcaccaag gtcgggatcg tcggggcggg cctcatggcc
     1321 agccagctcg cgctgctctt cgcgcagcgc ctcgaggtcc ccgtcgtgct gaccgacctc
     1381 gacgagcagc gggtagccaa gggcgtcggg tacgtccaca acgagctcga caagctggcc
     1441 ggcaaggggc ggctcagcag cgacaaggcg tcgcgctaca agagcctcgt gtccgggtcg
     1501 acgagcaagt ccgggttctc cgacgccgac ttcgtcatcg aggccgtctt cgaggagatg
     1561 ggcgtcaaga agcaggtctt cgccgaggtc gaggccgtcg tgcccgagac gtgcgtgctc
     1621 gccaccaaca cgtcgagcct gtcgatcacc gagatggcct ccgggctggc ctaccccggg
     1681 cgagtcgtcg ggttccactt cttcaacccg gtcgcggtga tgccgctcct ggagatcatc
     1741 accggcgagc agaccgacga cgccacgctg gcgacggcct tcgcgacggg caagtcgctg
     1801 cgcaagacgt gcgtcctcgt caaggactcg ccctcgttca tcgtcaaccg gctgctgggc
     1861 cggttcatgg gcgaggtcgg ccgcgtcgtc gacaacggca cacccgtcgc ggtggccgac
     1921 caggccttca ccggtctcgc gcccatgccg ccgttcgtcc tcatgggcct cgttggcccg
     1981 gccatcgccc tgcacaacag cgagaccctg gcccgcgcct tcccgggccg gttctacgtc
     2041 gccgagggtc tgcgccggat ggtcgaggcc aagaagtcga gcttctacac cttcgccggc
     2101 ggggagatgg tcgtcgaccc acaggcggcc gcgctgatgc cgtcgccccc ccagccggtg
     2161 gtcctgaccg cggccgaggt ccgtgaccag gtcctgtccg ggctggccga cgaggcccgt
     2221 cgcatgctcg acgagggcgt cgtccaggcg ccgatggacc tcgacctcgc cttgatcacc
     2281 ggtgccgggt tcgccttcaa caacggtggt ctgacgccgc tgctcgaccg ttccggcgtc
     2341 gccgagcggg tcaccggcag gcgcttccta cccccgggcg tggccagcgt cccggcctga
     2401 cccgggccgg gagagggtgc tgcccgcggt ggctggcgct gcgtcatacc ctgtcctatc
     2461 tcatatatga cgtaggatcg tgggcatgat tgagaacta
//
LOCUS       DATAIX010000720         2838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_141293, whole genome shotgun sequence.
ACCESSION   DATAIX010000720 DATAIX010000000
VERSION     DATAIX010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141293"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(358..846)
                     /locus_tag="VHM65_10625"
     CDS             complement(358..846)
                     /locus_tag="VHM65_10625"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2706204.1"
                     /translation="MRVPEFDDAAALRAWRDRTLYRRLMRAARAEASQTLAQIHQHGY
                     TDVSLTDTTLLANLDTDGCTITDLARRAGVTRQAASQQVAALVKSGYLHTRPSDTDGR
                     AVTVIQTDRGRALLRTALEIVARLETGYAAAIGERRVTELQKALDLLLSHIDPDGWLG
                     PP"
     gene            959..1357
                     /locus_tag="VHM65_10630"
     CDS             959..1357
                     /locus_tag="VHM65_10630"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX2706205.1"
                     /translation="MSTQTVDVVRSVYANFAKGEIEAVLAVLAPALEWVESPHDFLPH
                     RGTHRTPAAVAGKVFGMVMATFDEFAVVPELFHDAGDTVVVEGRSVGTTKQGRRLDAA
                     ACWVWTVRDGLVVANHNYHDTDAWRTALLG"
     gene            complement(1390..2574)
                     /locus_tag="VHM65_10635"
     CDS             complement(1390..2574)
                     /locus_tag="VHM65_10635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX2706206.1"
                     /translation="MTELRDVHLIDAVRTPFGKYRGALSSIRTDDLAATAIMALMARH
                     PGLDPARIEDVYFGNANGAGEDNRNVARMASLLAGLPVTVPGVTLNRLCASGAEAIIA
                     GGRAVATGDADLVLAGGVEGMSRAPYVLAKPDEALPRSMELHQTTVGWRMVNPKFPRE
                     WTASLGACAQDVATELRIGRDEQDEWAARSHQRVAAAYAAGKHEGFVVPVGDLAVDEC
                     LRPGSTVASLGTLKPAFSPEGSVTAGNSSPLSDGALAAFVGTRHTASVLGVEPFGQVV
                     ASATVGTDPHRFSVAPVGAIAKVLGRVGLSHADVDLWEINEAFAAMVLSTLHLMPPVD
                     RERVNVNGGAIAIGHPLGASAPRVVVDLCRELNRRGGGVGVAAACVGVGLGQAVVVRV
                     DA"
BASE COUNT          413 a         1002 c         1003 g          420 t
ORIGIN      
        1 gacctcggct tgtggctcag catcctgctc atcgtcgcct tgtccggcgg tctctacaac
       61 ctcttccgcc gccgcggctg gctctgacca gcccgctcac cgctcgatcc tggaaggctc
      121 gggggtcggc gccttcggcg ccgcgccgag ccgggggggt cgtccggcat ccggcctccg
      181 catcccgccg catccgaccg catccgcatc ctcgcgcagg ttagtggctc ctcgcgcggg
      241 aaatttgccg cgcgaggtgt gactaacctg cgcgaggatg ggtcgaaggc gcttggcggg
      301 ccttggcccg agccagggcg cgcatgtcct gcacaatcac gtgcacaaac gcagggatca
      361 cggtgggccg agccagccgt ctgggtcgat gtggctgagg aggagatcga gggccttctg
      421 cagctcggtg acgcggcgtt cgccgattgc ggccgcgtag ccggtctcca gtcttgcgac
      481 gatctcgagg gcggtgcgga gcagtgctcg cccgcggtcg gtctggatca cggtgaccgc
      541 acgaccgtcg gtatccgagg ggcgcgtgtg cagatagccg ctcttcacca gcgcggctac
      601 ctgttggctt gccgcctgcc gggtgacgcc ggcccgccgg gcgaggtcgg tgatggtgca
      661 tccgtcggtg tcgaggttgg cgagcagggt ggtgtcggtc agcgagacgt cggtgtaccc
      721 gtgctggtgg atctgcgcga gggtctggct ggcctcggct cgggccgcgc gcatcagcct
      781 gcggtagaga gtgcggtctc gccaggcccg cagtgcggcg gcatcgtcga actcaggcac
      841 cctcatgtac gcaacctcct tgacagtaat gcgcaagtca ctgtacaaaa tagagtcact
      901 gtacaaaatg gacgggtcga gggaaccgat gcacaggccc gggagaggga gaaccaccat
      961 gtcgacgcag accgtggacg tcgtgcgcag cgtgtacgcc aacttcgcca agggagaaat
     1021 cgaggccgtc ctggcggtgc tggcccccgc gttggagtgg gtcgagagtc ctcacgactt
     1081 cctgccccac cggggcacgc accggacacc ggccgcggtg gcgggaaagg tgttcgggat
     1141 ggtgatggcc accttcgacg agttcgccgt ggtccccgag ctgttccacg acgccggcga
     1201 caccgtcgtc gtcgagggcc ggtcggtcgg gacgacgaag cagggccgcc gcctggatgc
     1261 cgcggcctgc tgggtctgga ccgtgcggga cgggctggtg gtggccaacc acaactacca
     1321 tgacaccgac gcctggcgga ccgccctgct cgggtagagc cccttcaccg gtctgcattg
     1381 ccgcccgggc taggcatcga cgcggacgac gacggcctgg ccgagcccga cgccgacaca
     1441 cgccgccgcc acgcccaccc caccgccgcg gcggttgagc tcgcggcaca ggtcgacgac
     1501 gacccggggg gccgacgcgc cgagcgggtg cccgatcgcg atcgccccgc cgttgacgtt
     1561 gacccgttcg cgatcgaccg ggggcatgag atgcagggtc gacaggacca tcgccgcgaa
     1621 ggcctcgttg atctcccaca ggtccacgtc agcatgcgaa aggccaacac ggccaagcac
     1681 tttggcgatc gccccgacgg gcgccacgct gaaccggtgc gggtcggtgc cgaccgtcgc
     1741 cgaggcgacc acctgaccga acggctcgac cccaagaacg gacgccgtat ggcgcgtccc
     1801 gacgaatgcg gccagcgcac cgtcgctgag gggtgaggag ttgcccgcgg tgacgctccc
     1861 ctcggggctg aaggcgggct tgagcgtgcc gagcgacgcg accgtcgacc cagggcgcag
     1921 gcactcgtcg acggcaaggt ccccgaccgg gacgacgaac ccctcgtgtt tgcccgcggc
     1981 gtaggccgcc gccacgcgct ggtgcgagcg cgccgcccac tcgtcctgct cgtcccggcc
     2041 gatcctcagc tcggtcgcca catcctgcgc gcaggcaccg agggacgcgg tccactcgcg
     2101 cgggaacttc gggttgacca tccgccagcc gaccgtcgtc tggtgcagct ccatcgagcg
     2161 cggcagggcc tcgtcgggtt tggcgaggac gtagggcgca cggctcatgc cctcgacgcc
     2221 gccggccagg acgaggtccg cgtcaccggt cgcgaccgcc cgcccgcccg cgatgatcgc
     2281 ctcggccccg gaggcacaga gccggttgag ggtcaccccc gggacggtga ccggcagccc
     2341 ggcgaggagc gacgccatcc gggcgacgtt gcggttgtcc tcgcccgccc cgttggcgtt
     2401 gccgaagtag acgtcctcga tccgggccgg gtccaggcca gggtggcgcg ccatcagcgc
     2461 catgatcgcc gtggccgcga ggtcgtcggt gcggatcgag gacagagccc cgcggtactt
     2521 gccgaacggg gtccgcaccg cgtcgatgag gtggacgtcc cggagctcag tcatgacccc
     2581 attcttgtcc ctcacaccgc gccggcccac ccgcatgagg ccggtcagtg acgcatgtcg
     2641 cagcccgggt tcgggcggcc cgcctggagg atgatgaggc catggaccgt cccgcgacgc
     2701 gaccggaccg gaccctcgtc gcgatcctcg ccatcatcgc catcctcgtc atcagctcgc
     2761 tcgtcgtcgt gctcacgcgg cgcgcggcgg ccgtgctcga cccctccacg ccggccggaa
     2821 ccgtccaggc ctacgcgg
//
LOCUS       DATAIX010000721         1605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_260084, whole genome shotgun sequence.
ACCESSION   DATAIX010000721 DATAIX010000000
VERSION     DATAIX010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260084"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VHM65_10640"
     CDS             <1..223
                     /locus_tag="VHM65_10640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2706207.1"
                     /translation="NPCGGVSEVVGVAVLPSWRRRGVAAALTATLTRDALERGIATVF
                     CSAQSEQVARVYEGIGFRRIGTAALAEHT"
     gene            complement(166..981)
                     /locus_tag="VHM65_10645"
     CDS             complement(166..981)
                     /locus_tag="VHM65_10645"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX2706208.1"
                     /translation="MTGDEEIPLAGGNVSGAVRVGDTVRRPTGPWTPAVHAVLGHLAG
                     RLPHVPEVFGIDERGREILTFLPGEIVDIDSAVLTPGQLASLTGWTRRLHEAVAGFWH
                     DGPWRCFPVEHPSVIGHNDIAPYNACFDGDELVGVFDWDLAGPSTPLDELAFIAWNGV
                     PLWRDVGPQETAARLEIIASAYDGPTAGEILAAVPGRVQIVIDGIPAAAAAGDPGMAN
                     LMAIGEAERSGQSLDALQDRLPAIATALRDISGPAHVCSARAAVPIRRNPMPS"
     gene            complement(1029..1343)
                     /locus_tag="VHM65_10650"
     CDS             complement(1029..1343)
                     /locus_tag="VHM65_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2706209.1"
                     /translation="MLEVSVTDFAAAHQGGAAVIDVREPMEYVQGHVPGATLIPMSRF
                     TSMLDQLPQDPFYVICASGNRSYTVAQFLARQGRTALSVSGGTHAWQMRGYPLVQGMA
                     AG"
     gene            1446..1589
                     /locus_tag="VHM65_10655"
     CDS             1446..1589
                     /locus_tag="VHM65_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706210.1"
                     /translation="MVQIDPTESMAVLNRLRRAHGQRECLADGGGSPEGVRSMGKLFL
                     SFG"
BASE COUNT          238 a          559 c          583 g          225 t
ORIGIN      
        1 caacccgtgt ggcggggtga gcgaggtggt aggggtcgcg gtcctcccgt cctggcggcg
       61 ccggggagtc gcggcggcac tgacggccac cctgacccgg gacgccctcg agcgtggcat
      121 cgcgactgtc ttctgctcgg cccagtccga acaggtcgcg cgggtctacg agggcatcgg
      181 gttccggcgg atcgggacgg ccgccctggc cgagcacacg tgagcagggc ccgagatgtc
      241 gcgcagcgca gtggcgatcg cgggcaggcg gtcctgcagg gcgtcgaggg actggcctga
      301 ccgttcggcc tctccgatag ccatgaggtt cgccatccca gggtctcctg ccgccgcggc
      361 ggccgggatg ccatcgatca cgatctggac ccggcccggg actgcggcga ggatctcgcc
      421 cgcggtcggg ccgtcatacg cgctggcaat gatctcgagc cgggcggcgg tctcctgcgg
      481 tcccacgtca cgccagagtg ggacgccgtt ccaggcgatg aaggccagct cgtccagcgg
      541 ggtcgacggc ccggcaaggt cccagtcgaa gacaccgacg agctcgtccc cgtcgaagca
      601 cgcgttgtag ggcgcgatgt cgttgtgccc gatcacgctc gggtgctcga cggggaagca
      661 ccgccagggg ccgtcgtgcc agaacccggc gacggcctca tgaagccggc gggtccaccc
      721 ggtgagcgag gcgagctggc caggggtgag gaccgcggag tcgatgtcga cgatctcgcc
      781 aggcaagaag gtgaggatct cgcgcccgcg ctcatcgatg ccgaatacct cgggcacgtg
      841 gggaaggcga ccggccagat gacccaggac cgcgtggacc gccggcgtcc agggcccggt
      901 cggccggcgg acggtgtcgc ccacccggac cgcgccactg acgttgcctc cggcaagggg
      961 gatctcctcg tcgcccgtca ccgcaccacc ttgaccgggc cggcgccgtt ctgcaaggcg
     1021 accgcgcctc agccggccgc catgccctgc acgagcgggt agccgcgcat ctgccaggca
     1081 tgagtcccgc ccgagacgga cagcgccgtg cgcccctggc gcgccaggaa ctgggccacg
     1141 gtgtagctgc ggttgccact ggcgcagatc acgtagaacg ggtcctgggg cagctggtcg
     1201 agcatgctcg tgaaccggct catcgggatc agggtcgcgc cgggcacatg gccctggacg
     1261 tactccatcg gctcgcgcac gtcgatgacc gccgccccgc cctggtgggc tgcggcgaag
     1321 tccgtgacgc tgacttccag cactgacgcc tccttgctga cgagcggact gataccccct
     1381 ggggtatatt gaaccataac gtcggccaac cagggtccgg cgccaacggg tgaggaagtg
     1441 ccgcgatggt ccagatcgac cccaccgaga gtatggcggt cctcaaccgc ctgcgccgcg
     1501 cgcacggcca gcgggaatgc ctcgccgatg gcgggggatc gcccgagggg gtccgctcga
     1561 tggggaagct cttcctgtcc ttcgggtagc cgccgccccc cgcac
//
LOCUS       DATAIX010000722         2637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_141404, whole genome shotgun sequence.
ACCESSION   DATAIX010000722 DATAIX010000000
VERSION     DATAIX010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141404"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /gene="thrC"
                     /locus_tag="VHM65_10660"
     CDS             <1..1009
                     /gene="thrC"
                     /locus_tag="VHM65_10660"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX2706211.1"
                     /translation="LGEGGTPLIPAAYISALVGAEVFVKFEGVNPTGSFKDRGMTAAI
                     SVAARHGAKAVICASTGNTSASAAAYATKAGMTCGVLVPDGKISMGKLSQAIAHGATL
                     LQVDGSFDDCLNLARKLAEAYPVELVNSVNPARIEGQKTASFEVVDALGDAPDIHCLP
                     VGNAGNITAYWRGYREYHAGSDTPGPATRLPQMWGFQAAGSAPIVAGHPIDHPETIAT
                     AIRIGNPASWEQAVAARDDSGGRIEAVTDEAILAAHRILSAREGVFVEPASAASVAGL
                     LQVHAAGGVPPGARIVCTVTGHGLKDPQWALRTEDGSDIVPIRVPVDAFSAAAALGLE
                     G"
     gene            1006..2010
                     /gene="thrB"
                     /locus_tag="VHM65_10665"
     CDS             1006..2010
                     /gene="thrB"
                     /locus_tag="VHM65_10665"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX2706212.1"
                     /translation="MSVSLFPAERPTELPVGAGVRVRVPASSANLGPGFDSVGLALGI
                     WDTYDVHVDSHPGLWITVEGEGAGSVPTDSTHLVFRSMERAWAALAVAVPVGLHLHAH
                     NGVPHGRGLGSSATAIVAGIAAAHALAMASPGIPGPVALDLPAIAHLASILEGHPDNA
                     SASVYGGLTVSWTDDSDGLIRTVSPALHPDLHAVVFVPSGQLATELARKVLPTQVPLH
                     DAALNSGRAALLTLAVTQRPDLLLPATREWLHQEARRSALTGSMALVDALRGDGHAAV
                     ISGAGPSVLVLTTEPGRVWLPAELTESGGDWHRFEPGIPDEGASAHSTATHIEGFGEV
                     "
     gene            2332..>2637
                     /locus_tag="VHM65_10670"
     CDS             2332..>2637
                     /locus_tag="VHM65_10670"
                     /inference="COORDINATES: protein motif:HMM:NF019139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEX2706213.1"
                     /translation="MNVVALKGMASTLGITGTAKMRKGDLVAAIAARQSTSTDGDGPA
                     APQASHEHPQDRAEPRERSEERSQERPPREQQGRQQHTGRELNRAQDASQDQGNRPDQ
                     "
BASE COUNT          392 a          979 c          906 g          360 t
ORIGIN      
        1 cctgggggag ggcgggaccc cgctgatccc agcggcatac atctcagcgc tggtcggcgc
       61 tgaggtcttt gtcaagttcg agggcgtcaa cccgacgggg tcgttcaagg accgcggcat
      121 gaccgccgcg atatcggtgg ccgcgcgaca cggcgccaag gccgtgatct gcgcctcgac
      181 cggcaacacc agtgcgtcag cagccgccta cgccaccaag gccggtatga cgtgcggcgt
      241 gctcgtcccc gacggcaaga tctcgatggg caagctgtcg caggccattg cccacggtgc
      301 gaccctgctc caggtcgacg ggagcttcga cgactgcctc aacctcgccc gcaagctggc
      361 cgaagcatac ccggtcgagc tggtcaactc ggtcaacccg gcccgcatcg agggccagaa
      421 gaccgcgtcg ttcgaggtcg tcgacgcgct cggcgacgca cccgacatcc actgcctgcc
      481 ggtcggcaac gccggcaaca tcacggccta ctggcggggc taccgcgagt accatgccgg
      541 ctccgacacg cccgggccgg ccacgcgcct cccccagatg tgggggttcc aggccgcagg
      601 ctcggcgccg atcgtcgccg ggcacccgat cgaccacccc gagaccattg ccacggcgat
      661 ccggatcggc aacccggcct catgggagca ggcggtcgcg gctcgggacg actccggtgg
      721 gcggatcgag gccgtcaccg acgaggcgat cctcgccgcc caccgcattc tctcggcccg
      781 cgaaggggtc ttcgtcgagc ccgcctcggc cgcatcggtg gccgggctcc tgcaggtcca
      841 tgcggccggg ggagtccccc cgggggcgcg catcgtgtgc acggtcaccg gtcacggcct
      901 caaggacccc caatgggcgc tgcggaccga ggacggctcc gacatcgtcc cgatccgcgt
      961 tccggtcgat gccttctcgg cggcggccgc gctcggcctc gaaggatgag cgtgtccctc
     1021 ttccccgccg agcgccccac ggagcttccc gtgggggccg gcgtgcgggt gcgcgtgccc
     1081 gcgagcagcg ccaacctcgg tccggggttc gactcggtcg ggctggccct ggggatctgg
     1141 gacacgtatg acgtccacgt cgactcccac cccggcctgt ggatcaccgt cgagggggag
     1201 ggcgcagggt ccgtgcccac cgactccacc cacctcgtct tccgctcgat ggagcgcgcc
     1261 tgggcggcgc tcgccgtggc cgtgcccgtc gggctgcacc tgcatgccca caacggggtc
     1321 cctcatggtc gggggctggg ctcctcggcc acggcgatcg tcgcgggtat cgcggcggcg
     1381 cacgcgctcg cgatggcgag tcccggcata cccgggccgg ttgctctgga ccttccggcg
     1441 atcgcccact tggcctcgat cctcgaaggg caccccgaca acgcgtcggc cagcgtctat
     1501 ggcgggctca ccgtgtcgtg gaccgacgat tcggacggcc tgatccgcac ggtcagcccc
     1561 gccctgcatc cggacctgca tgcggtcgtc ttcgtcccgt ccggccagct cgcgaccgag
     1621 ctggcccgca aggtgctgcc cacccaggtg ccgctgcacg acgcggccct caactccggc
     1681 cgggctgccc tgctgaccct cgcggtgacc cagcggcccg accttctcct gccggccacc
     1741 cgtgagtggc tgcaccagga ggcccggcgg tcggccttga ccggctcgat ggccctcgtc
     1801 gacgcgctgc ggggcgacgg gcatgcggcc gtgatctcag gagccgggcc cagcgttctc
     1861 gtcctgacga ccgaaccggg tcgggtgtgg ctgcccgcgg agctgaccga gtcgggcggt
     1921 gactggcacc gcttcgagcc gggcatcccg gacgaggggg cctcggcgca ctcgaccgcc
     1981 acgcacatcg agggattcgg cgaggtgtga cagcggcggc gcaaacggtg ttagtctagt
     2041 caagcaccgc aagcgcgtgg ccgagtacag tcggcacgcc cgcgaggtgc acaccctcca
     2101 cgtctggacg agtcgactcg tccgatcgcc gtatgcgcgt cttccgtgga aaccccaatc
     2161 aggtcagcgc cgctggccaa gcacaccggt agcgcagtca gccctaccga cgacgagggg
     2221 gaaggatcct tcgtgacaga caccattgac gctgttgtga ctgccgtttc agaggcagac
     2281 gcgcccacag cgggcgccag cagcacccgc cgtgccggag ggctcgacac gatgaacgtc
     2341 gtggcgctca agggcatggc atcgacgctc gggatcaccg ggacggccaa gatgcgcaag
     2401 ggcgatctcg tcgcggccat cgcggcccgg cagtccacga gcaccgacgg cgacggccca
     2461 gccgctccgc aggcgtcaca cgagcaccca caggaccgtg ccgagccgcg ggagcgctct
     2521 gaggagcgtt cgcaggaacg cccgccgcgt gagcagcagg gccgccagca gcacaccggc
     2581 cgcgagctca accgcgccca ggacgccagc caggaccagg gcaaccgccc ggatcag
//
LOCUS       DATAIX010000723         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_260141, whole genome shotgun sequence.
ACCESSION   DATAIX010000723 DATAIX010000000
VERSION     DATAIX010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260141"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..1395)
                     /locus_tag="VHM65_10675"
     CDS             complement(253..1395)
                     /locus_tag="VHM65_10675"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HEX2706214.1"
                     /translation="MNDSAFRPPGPQTELERRLRAALGDSAARVAPTDRRSSILSAAR
                     DDESLSDLPGAGGRRWFALAAAAAVLLLVSGLVWLGGRPGRVATVAASTTTPTPSVSA
                     SEPSPVPTTPAATRVPTSSATPAVLSIAMPVYFVGLPAGPRGSPMLYREFLRVRSAGP
                     LAADDLATRAVAAALRPGTSAPGVTYLDLWAGTGVVSVELVSISGGHEIHVTLTGTGQ
                     SGLSGDQAKAAVGSVVWTATAAYSLGSIPVRITVADGSTTLFGRYPLTGTTSRPPTDT
                     WPSVLAPLWISAPSRGDVLGRGTRVVVTGESSTFERGSLHWTLSRDGSVVRTGTTPSG
                     AAGPLRGPFRLDLGVLDPGSYELACFEPSAKDGSAYGTVTTVFSVR"
     gene            complement(1392..1952)
                     /locus_tag="VHM65_10680"
     CDS             complement(1392..1952)
                     /locus_tag="VHM65_10680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEX2706215.1"
                     /translation="MRTLAVMEPPTRRTSPDADAALTEVYDAHWVSLVRLAWLLLRDQ
                     SRAEEVVQDAFIATYPRIAELRASGGAVAYLRRSVVNGCRSVQRRQGVERRYLRGHLE
                     SVDLPGRRADASAESGALDHDAHAHMVAALQQLPRRQREVLVLRYYLDLSEAQIADAL
                     AISPGAVKSHASRGLATLRTLVEGRG"
     gene            2016..>2351
                     /locus_tag="VHM65_10685"
     CDS             2016..>2351
                     /locus_tag="VHM65_10685"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX2706216.1"
                     /translation="MSADPECLFCRIVSGTVPASIIAQTEHSVAFRDINPQAPVHLLV
                     IPRRHIVTVTELAELAPGEAADLCALAGRVARAEGVSDGFRLVANSGATAGQVVFHAH
                     LHVLGGRPMG"
BASE COUNT          352 a          846 c          859 g          294 t
ORIGIN      
        1 cgaccgcgcg ggtcgagcct gatggcgatg agacgtatgg ggcaggcgag acggctagcg
       61 cgccgccgct atacctgatg acgccgcgcc ctgaggatcg gggagcgttg agggggtcag
      121 gacaacgcca acactcccca caaacggcga ttgccccgtc aacgataccg atgagacgta
      181 tggggcgggt gaggccggta agggggttgc ggcggcgatg gagataccgg gcgcggttgg
      241 tccttggccg cgctagcgca ccgagaacac cgtcgtcacc gtgccgtacg ctgacccgtc
      301 cttcgcgctc ggctcgaagc acgcgagctc gtaggagccg gggtcgagca ccccgaggtc
      361 gagccggaac ggcccgcgca gcggcccggc cgcgccagac ggggtcgttc cggtgcgcac
      421 gacggacccg tcgcgcgaca gggtccagtg cagcgagcct cgctcgaacg tcgaggactc
      481 gccagtcacg acgacgcgcg tcccccggcc cagcacgtcc ccacggcttg gcgcgctgat
      541 ccacagcggt gcgagcacgc tcggccacgt gtccgtgggg ggccggctcg tcgtgccagt
      601 gagggggtac cggccgaaca gggtcgtcga cccgtcggcc acggtgatcc ggaccgggat
      661 cgagcccagg ctgtaggccg ccgtcgccgt ccagacgact gaccccaccg ccgccttcgc
      721 ctggtcgccc gacaggccgg actgaccggt ccctgtcaag gtcacgtgga tctcatgacc
      781 accgctgatc gacacgagct cgaccgacac cacgccggtc cctgcccaca ggtcgagata
      841 cgtgacgccg ggtgcggacg tcccgggacg cagcgcggcc gccacggcac gggtggcgag
      901 gtcgtcggcg gcgagcggac cggccgagcg gacccgcagg aactcgcggt agagcatcgg
      961 cgacccgcgt ggcccggcgg gcaacccgac gaagtagaca ggcatggcga tcgacaggac
     1021 ggcgggtgtc gccgaggagg tgggcacccg cgtcgccgca ggggtggtcg gcacggggct
     1081 cggctccgac gcgctgacac tcggggtcgg tgtcgtcgtc gaggcggcca ccgtcgcgac
     1141 gcgccctggc cgtccgccga gccagaccag cccggacacg agcaggagga ccgccgcggc
     1201 ggccgcgagg gcaaaccagc gccgcccgcc agcaccgggc agatccgaga gcgactcgtc
     1261 gtcgcgagcg gccgaaagga tcgagctgcg gcggtcagtg ggggcaaccc gggcggccga
     1321 gtcgccaagg gcggcacgca gcctgcgctc gagctcggtc tgcggccctg ggggccggaa
     1381 agcgctgtcg ttcatcctcg tccttccacc aacgtacgca gcgtcgccag cccgcgcgag
     1441 gcatgcgact tgaccgcccc gggggagatg gcgagggcgt cggcgatctg agcctcggac
     1501 aggtccaggt agtagcgcag cacgagcacc tcgcgctgac gccggggcag ctgctgcagc
     1561 gcagcgacca tgtgggcgtg tgcgtcatgg tcgagtgccc cggactccgc gctcgcgtcc
     1621 gcccgccgtc caggcaggtc gacgctttcg aggtgcccac gcaggtagcg ccgctccacc
     1681 ccctggcggc gctggaccga ccggcagccg ttgacgacgc tgcgccgcag gtaggccacg
     1741 gcgccacccg aagcccgcag ctcggcgatc cgcgggtagg tcgcgatgaa ggcgtcctgc
     1801 accacctcct cggcgcgcga ctggtcgcgc agcagcagcc aggccagacg cacgagcgag
     1861 acccagtgcg cgtcatacac ctcggtcagt gccgcgtcgg cgtcaggcga ggtccggcgg
     1921 gtcggtggct ccatcaccgc caacgttctc atgccggtat gacgcgcagc gtgcgctccc
     1981 ggttgacccg cttgggccgg gctacgctgg ccgccatgag tgccgacccc gagtgcctct
     2041 tctgccggat cgtgtccgga accgtcccgg cgagcatcat cgcgcagacc gagcacagcg
     2101 tcgccttccg cgacatcaac ccgcaggctc cggtccacct cctggtgatc ccgcggcgcc
     2161 acattgtgac ggtcaccgag ctcgccgagc tggctccggg cgaggccgcc gacctgtgtg
     2221 ccctggcggg ccgagtcgcg cgggccgagg gggtgagcga cgggttccgg ctcgtggcca
     2281 actccggcgc cacagccggc caggtggtct tccacgccca cctgcacgtc ctcggcggtc
     2341 gcccgatggg c
//
LOCUS       DATAIX010000724         7380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_141725, whole genome shotgun sequence.
ACCESSION   DATAIX010000724 DATAIX010000000
VERSION     DATAIX010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 7380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7380
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141725"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VHM65_10690"
     CDS             complement(<1..694)
                     /locus_tag="VHM65_10690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX2706217.1"
                     /translation="MSPRLVSVGLLLPTSAAAAIVLTGSASPAHTQTHLALSSAELAQ
                     GAASSGASARASAALADRVADLRAARDSEREAWRIARAVRTKASAAANKAREFALTRT
                     DAGAPGSHLWVNPFSNYSYSSPFGFRWGRLHAGIDLAGPVGTRVRAMSSGTVVFAGDR
                     GGYGTNVEIKYWDGSVSWYAHLSSVEVTIGDTVNAGEIVARSGNTGHSTGPHLHLESI
                     PAAARPRSTPCPG"
     gene            904..1311
                     /locus_tag="VHM65_10695"
     CDS             904..1311
                     /locus_tag="VHM65_10695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX2706218.1"
                     /translation="MTDSPLRIVVAKPGLDGHDRGAKVVARALRDAGMEVVYTGLHQT
                     PDQIVEAAIQEDADGVGLSVLSGAHMTLFAKVIERLRERDAEDITVFGGGIIPEADIA
                     ELTALGVAKVFTPGATTTEIVAWVREHVGPARV"
     gene            1812..3011
                     /gene="sucC"
                     /locus_tag="VHM65_10700"
     CDS             1812..3011
                     /gene="sucC"
                     /locus_tag="VHM65_10700"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX2706219.1"
                     /translation="MDLFEYQARDMFEAHGVPVLDGAVARTPAEARAAAERIGAKSGG
                     VTVVKAQVKTGGRGKAGGVKVARSAQEAETLAGQILGMDIKGHRVNVVMIAQGAQIAE
                     EYYFSVLLDRANRSYLAMCSKEGGMEIEQLAVERPDALARVPVDPIVGIDAAKAREIV
                     DAAGFDEADRAPIAAAIEALWTVYRDEDATLVEVNPLVKTTDGLIVALDGKVSLDANA
                     GFRHPDHAELEDTTATDPLEAAAKEKDLNYVKLDGSVGVIGNGAGLVMSTLDVVAYAG
                     EEFTHADGTHPKPANFLDIGGGASAEVMADGLHIILSDPQVKAVFVNVFGGITSCDSV
                     ANGIVGALDALGDEEDRPLVVRIDGNNVEEGRRILAERNHPRVTIVDTMDGAARMAAE
                     LAAAATN"
     gene            3025..3924
                     /gene="sucD"
                     /locus_tag="VHM65_10705"
     CDS             3025..3924
                     /gene="sucD"
                     /locus_tag="VHM65_10705"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF004230.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX2706220.1"
                     /translation="MAIFLDDTSKVIVQGMTGSEGMKHTTRMLASGTTIVGGVNPRKA
                     GTTVDFPGGVQVPVFGSVQEAVDATGADVTVIFVPAAFTKSAVVEAVDAKVPLAVVIT
                     EGVAVKDTAEFYTYAQQSGTTRLIGPNCPGLISPGRANAGIIPADISGPGRVGLVSKS
                     GTLTYQMMYELRDYGFSSAIGIGGDPIIGTTHIDCLAAFEADPETDAIVIIGEIGGDA
                     EERAAAYIKEHVTKPVVGYVAGFTAPEGKTMGHAGAIVSGSSGTAQAKQEALEAAGVK
                     VGKTPSETAGLMREIMQGLSQDS"
     gene            4050..5342
                     /locus_tag="VHM65_10710"
     CDS             4050..5342
                     /locus_tag="VHM65_10710"
                     /inference="COORDINATES: protein motif:HMM:NF040070.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6350 family protein"
                     /protein_id="HEX2706221.1"
                     /translation="MPVLDRIAPTGSTDATTFTSGGVGGGWARPALLGAAAAVLSLVV
                     VLAPALLTWVGSPDPGQPWTSAAGVGVSLWLLGHGAPLRAGPVTVSLVPLLLLAINAG
                     IAHSCARFAVRHWRTGSALTRLRVRSDVLEGLGSFVGGYALIALTGSLATLGFPLHAH
                     PAVAVLATALVSSAGALSALVRDRGDDLTDLVPLVAPVMAHERWPLLRSTSRVAAEAV
                     GVVLALGLAVTLAVLALGWDRVSAIHGVLDPGGPGGVGLVLAQLALLLNVALWGLAWL
                     AGPGFSVGVGSVISLGGSAPGLMPMVPFLGALPSEGSNPPWLRGLVLLPVLAGAFLAW
                     RLDRAEPFRSWSGAGARCALAAGLAAAAVAGLSAIGTGAVGVARLAHVGPSWVSLTGA
                     LLAELLAGAVATAGLLVRHRQGRRSLADSGSGAGDDPS"
     gene            5412..6008
                     /gene="purN"
                     /locus_tag="VHM65_10715"
     CDS             5412..6008
                     /gene="purN"
                     /locus_tag="VHM65_10715"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX2706222.1"
                     /translation="MSDVVVLVSGTGTLLQALLDATADPGYGVRIVAVGSDRPAIEGL
                     ARAERAGLPTFVERVEGHADRDAWDRALAARIASYEPMFVVSAGFMRILGPSVLAGHT
                     VINTHPALLPAFAGAHGVRDALAYGVRVTGCTCHVVDAGVDTGPIIEQRAVVVEPGDD
                     EASLHERIKVVERDMLVEVVRRMAGGWLINGRKVELGG"
     gene            6001..>7380
                     /gene="purH"
                     /locus_tag="VHM65_10720"
     CDS             6001..>7380
                     /gene="purH"
                     /locus_tag="VHM65_10720"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX2706223.1"
                     /translation="MADAQTQEVQGRRPIRRALVSVYDKTGLAELAVALHEAGVSIVS
                     TGSTAATIAAAGAAVTPVEELTGFPECLDGRVKTLHPKVHAGILADTHNPEHVAQLAD
                     LGVEAFDLVVSNLYPFTATVVSGAPPQECVEQIDIGGPSMVRAAAKNHASVAIVTSPA
                     SYPMVVEALALGGFTLAQRQRLAAEAFVRTATYDVAVASWMGNVVTDTSDGTGFPAWV
                     GATWDRAAVLRYGENPHQRAALYGNGFQPRPGLAQARQVHGKEMSYNNYLDADAAVRA
                     AYDQGDQPTVAVVKHANPCGIAVGPDIAAAHRRAHECDPVSAFGGIIATNRPVTAAMA
                     QTVKDIFTEVVVAPAFDADALDILTAKKSIRLIECPAPGRGGVEIRPIWGGLLMQARD
                     AIDASGADGAGRPTGDDPARWRLVAGSSAGPATLADLAFAWRAVRAVRSNAILLARDV
                     ASVGIGMGQV"
BASE COUNT         1020 a         2607 c         2634 g         1119 t
ORIGIN      
        1 atccagggca tggggtcgac cgcggtcttg ctgccgccgg gatggattcc aggtgcaggt
       61 gagggccggt ggagtgaccc gtgttgccgc ttctggccac gatctcgccc gcgttgaccg
      121 tgtcaccgat cgtgacctcg accgagctga gatgggcgta ccacgagact gacccgtccc
      181 agtacttgat ctcgacattc gtgccgtagc cgccccggtc cccggcaaag acgaccgtgc
      241 cactggacat ggcccggact cgcgtcccga cggggccggc gaggtcgatg cccgcgtgca
      301 gccgccccca ccggaagccg aagggggagg agtaggagta gttcgagaag gggttgaccc
      361 acaggtggct gcccggggcg ccggcgtcgg tgcgggtcag cgcgaactcg cgggccttgt
      421 tcgcggccgc ggacgccttg gtccgcaccg cgcgggcgat ccgccaggcc tcccgctcgc
      481 tgtccctggc cgctcgcagg tccgcgaccc ggtccgcgag agctgcgctc gcgcgggctg
      541 aagcaccgga ggacgcagcg ccctgggcga gctcggcact ggacagggca aggtgggtct
      601 gggtgtgcgc cgggctggcc gagcccgtga ggacgatggc ggccgcggcg gatgtcggca
      661 gcaggaggcc gacgctgacc agccgtggcg acaccagcga tcgggacttc ttgcggtgga
      721 gtccggcgtg ggcgcggtgc cgaccggcag gcgagcgttg atgcacgagg tggggggtcc
      781 ttcgcggttc ggggatttcg acaaccctag cgtgaccgtt tcgttacata caaactggtc
      841 gcggagcgga ccctgttacc tcgctcacgc gccgggccgc ggcggagtcg atagtctgca
      901 ggtatgactg actccccact gcgcatcgtg gttgccaagc ctggtctgga cggccatgac
      961 cggggcgcca aggtcgtcgc ccgagccctg cgtgatgcgg gcatggaggt cgtctacacg
     1021 ggactgcacc agaccccgga ccagatcgtc gaggcggcga tccaggagga tgccgacggg
     1081 gtcggcctgt cggtcctgtc cggcgcccac atgacgctgt tcgccaaggt gatcgagcgc
     1141 ctgcgtgagc gcgatgccga ggacatcacc gtcttcggcg gcgggatcat ccccgaggcc
     1201 gacattgccg agctcaccgc tttgggggtg gccaaggtgt tcacccccgg ggcgacgacg
     1261 accgagatcg tcgcgtgggt ccgcgagcat gtcggccccg cccgcgtgtg agctgttcgt
     1321 caccgccctc ggtgatgtcc gatttacccg ctctgtcgcg cccgccgagg ccctttaggt
     1381 ctggtttagg cctggaatac cgggaaagag taaagaaatg acaagtctcg ttgacccatg
     1441 cctcagtctc gggtttccta gtggtagtac ctgaacgacg tgccccccac acgccgccgg
     1501 gtgcgagcca ggtgcaagag aagagagcgg gctgactccc cccaggtccc cgttcgccgc
     1561 agttctgcgg agcgacttga gtcgccactc tcgcgtccga ggcgttggac tgtggggccg
     1621 gacacgacgg tgctcggccc cacagctgtc cctggaccca ccgcgcggcg ccgggtcact
     1681 cccgctcggc gtgagatcgg tcccatcggg tgcctggacc gcgccggggt caatggcgtt
     1741 gactaggctc acgctggagt cgtcgcagag ctgacgacag tcccttcgac gaggatgagg
     1801 acgggatctg cgtggatctt ttcgagtacc aggcgcgcga catgttcgaa gcccacggcg
     1861 tccccgtgct ggacggggcc gtggcgcgga ccccggccga agcccgtgcg gctgccgagc
     1921 gcatcggggc caagagcggg ggcgtcaccg tcgtcaaggc tcaggtcaag accggaggtc
     1981 gtggcaaggc cggcggggtc aaggtcgccc ggtccgcgca ggaggccgag acgctcgccg
     2041 ggcagatcct cgggatggac atcaagggcc atcgggtcaa cgtcgtcatg atcgcgcagg
     2101 gcgcccagat cgcggaggag tactacttct cggtcctgct ggaccgggcc aaccgctcct
     2161 acctggccat gtgcagcaag gaaggcggga tggagatcga acagctcgcc gtcgagcggc
     2221 ccgacgcctt ggcccgggtc ccggtcgacc cgatcgtcgg gatcgacgcg gcaaaggcac
     2281 gcgagatcgt cgacgccgcg gggttcgacg aggccgaccg agcgccgatc gcggcggcca
     2341 tcgaggcgct gtggacggtc taccgcgacg aggacgccac cctggtggag gtcaaccccc
     2401 tcgtcaagac gaccgacggc ctgatcgtgg ctctcgacgg caaggtcagc ctcgacgcca
     2461 acgcgggctt ccgtcaccct gaccacgccg agctcgagga caccaccgcc accgacccgc
     2521 tcgaggccgc cgccaaggag aaggacctca actacgtcaa gctcgacggc agtgtcggtg
     2581 tcatcggcaa cggcgcgggc ctggtcatgt cgacactcga cgtcgtcgcc tacgcgggcg
     2641 aggagttcac ccacgccgac ggcacccatc ccaaacccgc caacttcctc gacatcggcg
     2701 gcggcgcgtc ggccgaggtc atggccgacg gtctgcacat catcctgagc gacccccagg
     2761 tcaaggcggt cttcgtcaac gtcttcggtg gcatcacgtc gtgcgactcg gtcgccaacg
     2821 gcattgtcgg cgccctcgac gcgctcggtg acgaggagga ccgtccgctg gtcgtgcgca
     2881 tcgacggcaa caacgtcgag gagggccggc gcatcctcgc cgaacgcaac cacccccggg
     2941 tgaccatcgt ggacaccatg gacggcgcgg cccggatggc cgccgagctc gccgccgcgg
     3001 cgacgaactg aagggggcga gatcatggcg atcttcctcg atgacacctc gaaggtcatc
     3061 gtccagggca tgaccggctc ggaaggcatg aagcacacga cgcgcatgct cgcctcgggc
     3121 acgaccatcg tcggcggcgt caacccgcgc aaggcgggca cgacggtcga cttccccggc
     3181 ggcgtccagg tgccggtctt cggcagcgtc caggaggccg tcgacgcgac cggcgccgac
     3241 gtcaccgtca tcttcgtccc cgccgccttc accaagtcgg ccgtcgtcga ggcggtggat
     3301 gccaaggtcc cgctcgccgt tgtcatcacc gagggcgtcg cggtcaagga cacagcggag
     3361 ttctacacct atgcccagca gtccggcacg acccggctca tcggtccgaa ctgtcccggc
     3421 ctgatcagcc ccggccgcgc caacgccggc atcatcccgg ccgacatcag tggcccgggc
     3481 cgggtcggcc tcgtgtccaa gtcgggcact ctgacctacc agatgatgta cgagctgcgt
     3541 gactacggct tctcctcggc catcggcatc ggtggcgacc cgatcatcgg gaccacccac
     3601 atcgactgtc tcgcggcgtt cgaagcggac cccgagaccg acgccatcgt catcatcggc
     3661 gagattggcg gcgacgccga ggagcgcgcg gcggcataca tcaaggagca cgtgaccaag
     3721 ccggtcgtcg gctacgtcgc cggcttcacc gcccccgagg gcaagacgat ggggcacgca
     3781 ggggccatcg tctcgggttc gtcaggcacg gcccaggcca agcaggaggc cctcgaggcc
     3841 gccggtgtca aggtcggcaa gacgccctcg gagaccgccg ggctcatgcg cgagatcatg
     3901 cagggcctga gccaggactc ctagcgccac accccccgtc cggtatgacg cccagctcac
     3961 cccgcggcgg gctgggcatc ggcgacttcg ccgttccccg gcgcgtccat ccgcccccgg
     4021 ccaggcggac cagcgacgat aggcgcacca tgcctgtcct cgaccgcatt gcccccaccg
     4081 gctccaccga tgcgaccacc ttcacctccg gcggcgtcgg tggtggctgg gcgcgtccgg
     4141 cgctgctcgg tgccgctgcg gcggtcctgt ccctcgtggt cgtcctggct ccggcgttgc
     4201 tcacctgggt ggggagcccc gacccgggcc agccctggac gagcgcagcg ggggtcggtg
     4261 tcagcctgtg gctgctcggg catggcgccc cgctgcgggc aggaccggtc accgtgtcgc
     4321 tggtcccgct gctcctcctt gccatcaacg ccgggatcgc ccacagctgt gcccggtttg
     4381 ccgtccggca ctggcggacc gggagcgcgc tgacccggct gcgggtccgg tcagatgtcc
     4441 tggagggtct tggctcgttc gtcggggggt atgccctcat tgccctgacc ggctcgctgg
     4501 ccacgctcgg tttccccctg cacgcgcatc ccgcggtggc cgtgctcgcg acggcgctcg
     4561 tgtcctcggc gggagccctc agcgcgctcg tgcgggaccg cggcgacgac ctcaccgacc
     4621 tggtgcccct cgttgcgccg gtcatggccc acgaacggtg gccgctgctg cgctccacca
     4681 gccgggtcgc ggccgaggcc gtcggcgtcg tcctggcgct cgggctcgcc gtcaccctcg
     4741 ccgtcctcgc cctcggctgg gaccgggtca gcgccatcca cggcgtgctc gaccccgggg
     4801 gtcctggcgg ggtcgggctc gtcctcgctc agctggccct gctgctcaac gtcgcgctct
     4861 ggggcctggc ctggctggcc gggcccgggt tcagcgtcgg cgtgggatcg gtgatctccc
     4921 tcggcgggtc agcgcctggg ctcatgccga tggtgccctt cctcggtgcg ctgccctcgg
     4981 aagggtcgaa cccgccctgg ttgcgggggc tcgtcctgct gccggtcctg gccggcgcct
     5041 tcctggcctg gcgcctcgac cgggccgagc cgttccgctc ctggtcggga gccggcgcgc
     5101 ggtgtgccct cgctgccggg ctcgcggcgg cagcagtcgc cgggctgagc gcgattggca
     5161 cgggcgccgt cggggtggcc aggctcgctc atgtcggccc gtcctgggtg tccctgaccg
     5221 gcgcgctgct ggccgagctg ctcgccgggg cggtcgccac cgccggcctg ctcgtccggc
     5281 atcggcaggg ccgccggtcg ctggccgact ccgggtccgg ggccggcgac gacccgagct
     5341 gagtccgccg cgcactcgtg cgacgggctg atgggttccg ggtccggcct tttccggtag
     5401 ggtccgcaaa cgtgagcgat gtcgtcgtcc tcgtctcggg gaccgggacg ctccttcagg
     5461 cactcctcga cgccaccgcc gatccggggt atggcgtgcg catcgtcgcc gtcggctctg
     5521 accgtccggc catcgagggc ctggcccgag ccgagcgcgc cggcctcccc accttcgtcg
     5581 agcgggtcga gggccacgcc gaccgggatg cctgggaccg tgcgctagcc gcccggatcg
     5641 cgtcatacga gccgatgttc gtcgtctcgg cggggttcat gaggattctt gggccgagcg
     5701 tcctggccgg ccacaccgtc atcaacacgc acccggcgct gttgcccgcg tttgcgggcg
     5761 cgcacggcgt ccgggacgcg ctggcgtatg gcgtgcgggt caccggctgc acctgccacg
     5821 tggtcgatgc gggcgtcgac accgggccga tcatcgaaca gcgggccgtc gtcgtcgaac
     5881 ccggcgatga cgaggcgagc ctgcacgagc ggatcaaggt cgtcgagcgg gacatgctgg
     5941 tcgaggtcgt tcgccggatg gcgggcggct ggctcatcaa cgggaggaag gtcgagctcg
     6001 gtggctgacg cgcagacgca ggaggtgcag gggcgccggc ccatccgccg ggcgctcgtc
     6061 tcggtctacg acaagactgg tctggccgag ctggcggtcg cgttgcacga ggcgggcgtg
     6121 tcgatcgtgt ccaccggctc gaccgccgcg accattgccg ctgctggggc tgcggtgaca
     6181 ccggttgagg agctgacggg cttccccgag tgcctggacg gccgggtcaa gaccctgcac
     6241 cccaaggtcc atgccgggat cctcgccgac acccacaacc ccgagcacgt ggcgcagctc
     6301 gccgacctcg gggtcgaggc gttcgacctc gtcgtgtcca acctctaccc cttcaccgcg
     6361 accgtggtct cgggggcgcc gccgcaggag tgcgtcgagc agatcgacat cggcggcccc
     6421 tcgatggtcc gggccgcggc caagaaccat gcgagcgtcg cgatcgtcac cagccccgcg
     6481 tcatacccga tggtggtcga ggcgctcgcg ctgggcggct tcacgctcgc gcagcggcaa
     6541 cggctcgcgg ccgaggcctt cgtgcgcacc gcaacgtatg acgtcgccgt cgcctcgtgg
     6601 atgggaaacg tcgtcaccga cacctccgac ggcacgggct tccccgcgtg ggtcggcgcc
     6661 acgtgggacc gcgctgcagt gctgcgctat ggcgagaacc cgcaccagcg ggcggccttg
     6721 tacggcaacg gtttccagcc tcggccgggc ctggcgcagg cgcgccaggt gcacggcaag
     6781 gagatgtcct acaacaacta cctcgacgcg gacgcggccg tgcgggcggc gtacgaccag
     6841 ggcgaccagc cgacggtggc ggtcgtcaaa cacgccaacc cgtgtgggat cgcggtcggc
     6901 ccggacatcg cggcggccca tcgcagggcg cacgagtgcg acccggtgtc ggccttcggg
     6961 gggatcatcg cgacaaaccg gcccgtgacc gccgccatgg cgcagaccgt caaggacatc
     7021 ttcaccgagg tcgtcgtggc cccggccttc gacgccgacg cgctggacat cctcaccgcc
     7081 aagaagtcca tccggctgat cgagtgcccc gccccagggc ggggtggggt cgagatccgg
     7141 ccgatctggg gcgggctgct catgcaggca cgggacgcga tcgacgcctc cggtgcggac
     7201 ggcgcgggcc gcccgaccgg tgacgacccg gcgaggtggc ggctcgtcgc cggctcctcg
     7261 gccggtccgg cgaccctggc cgacctggct ttcgcctggc gggcggtgcg ggcggtgagg
     7321 tcgaacgcga tcctgctcgc ccgcgacgtc gcctcggtgg ggatcgggat gggccaggtc
//
LOCUS       DATAIX010000725         3463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_141773, whole genome shotgun sequence.
ACCESSION   DATAIX010000725 DATAIX010000000
VERSION     DATAIX010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141773"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="VHM65_10725"
     CDS             complement(<1..1099)
                     /locus_tag="VHM65_10725"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2706224.1"
                     /translation="MTPSADRIRPITVTIGGIPDLPDIVGRDRQVDTLVGLLDGGQSA
                     VLTGDRRMGKTSVARLVEARLLADGRRVARVSAERETLDGFVDALTDALDRVTPGSRI
                     RSELDRWGLRVKAGPVEAKRTAAHRTLDRIVEAALPEAGERPLVLIVDEIPVLAQMLE
                     RQAKGSGAEVLVTLRRLRQEGSGRISMLLLGSVGFHHVSDSAPGEVNDVSKEPVGALS
                     VADATYLARCLLLGEQVPTTDDRAVAGAAVAAAEAMPFYVQHLVKACRNLSASTGHPV
                     HPEDVDTLVEVALTDPDDPWELRHYRRRVPSYYGPGNEELITAILDSYADADGFLGVD
                     AVLRQVASTGVAPTLTRRQLVDRVEKLEQDHY"
     gene            1241..1399
                     /locus_tag="VHM65_10730"
     CDS             1241..1399
                     /locus_tag="VHM65_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706225.1"
                     /translation="MPNALTGDFDAVLELSGGTLNRLVATMHQNGADSGKPSLPHVAY
                     FRLGDDRA"
     gene            1684..2928
                     /locus_tag="VHM65_10735"
     CDS             1684..2928
                     /locus_tag="VHM65_10735"
                     /inference="COORDINATES: protein motif:HMM:NF017617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEX2706226.1"
                     /translation="MTDQPSTPSSDAAIAPLEPPSSTLTLTAPAPPKAVAETSAPKMA
                     PAVPEAALPGLDSKVDSYLGQLLAAPAKSPEFEAKATDVRTMGDADIRRAAESSNRLL
                     QSPVKALQEGGLSEGSKVGATLLDLRRTVEDLDPAQATGVKKVLGMIPFGDKVTDYFR
                     RYQSAQSHLNGILHALREGQDELTKDNTALNLEKQQLWDTMGMLNQYVYVAERLDSRL
                     AASIATLDVSDPDKARSLRDDVMFYVRQKHQDLLTQLAVSIQNFLAIDIVIKNNLELI
                     KGVDRASTTTISALRTAVIVAQALGNQKLVLDQITALNVTTSGLIERTSEMLKDNSVK
                     IQEQAASATIGLPQLQRAFDNVYQTMDAIDAFKVQALDSMAATIGTLEGEVVKSRSYL
                     DRVQRRDQRIASGSLDITSLGR"
     gene            2928..>3463
                     /locus_tag="VHM65_10740"
     CDS             2928..>3463
                     /locus_tag="VHM65_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706227.1"
                     /translation="MGIGDWLGWGKAEPAPPVHAVPTSEHLIEALTRVETLARQGAVP
                     GPVMSRLARVTRVVRDTIPRLDALGAGSVSAYNVMATATDYLPEAIGGYLRLPRQWAD
                     SRPVDRGKTSLMILIDQLDLLGATMDKVFDAVCRSDAEALVVHGRFLQEKFGSPSGGS
                     LALQQVAAPPAPDPTSTSP"
BASE COUNT          568 a         1253 c         1158 g          484 t
ORIGIN      
        1 ggtagtggtc ctgctcgagc ttctcgacac ggtcgaccag ctggcggcgg gtcagcgtgg
       61 gcgcaactcc cgtcgaagcc acctgacgga gcaccgcgtc gacaccgagg aagccgtcag
      121 cgtccgcata gctgtcgagg atcgccgtga tgagctcctc gttcccgggc ccgtagtagg
      181 aggggacccg tcgccggtag tgccgcagct cccaggggtc gtcggggtcg gtgagcgcga
      241 cctcgaccaa ggtgtcgacg tcctcagggt ggaccgggtg gccggtcgac gcgctcaggt
      301 tccggcaggc cttgacgagg tgctggacgt agaacggcat cgcttcggca gcggccaccg
      361 ctgccccggc cacggctcgg tcatcggtcg tgggcacctg ttcgccgagc aacaggcatc
      421 tggccaggta tgtcgcgtcc gcgacactga gcgcaccgac cggctccttg ctcacgtcgt
      481 tgacctcgcc gggagcggag tcactcacat ggtggaagcc gaccgacccc agcaggagca
      541 tcgagatgcg gccggagcct tcctggcgaa gccgccgcag cgtgacaaga acctccgccc
      601 ctgacccctt ggcctggcgc tccagcatct gggccagcac cgggatctcg tcgacgatga
      661 ggacgagcgg ccgctcgccg gcctcgggaa gggcggcctc gacgatccgg tcgagcgtgc
      721 gatgcgccgc cgtgcgcttg gcctcgaccg gcccggcctt gaccctgagc ccccaacggt
      781 ctagctcgct gcggatccgg ctgcccggcg tgacccggtc cagggcgtcc gtcaacgcgt
      841 cgacgaaacc atcgagcgtc tcacgctccg cgctgaccct tgccactcgg cgcccgtcgg
      901 ccaggagccg agcctcgacg agccgcgcga ccgacgtctt gcccatgcgc cggtcgccgg
      961 tgagcacggc gctctgccca ccgtcgagga gccccacgag agtgtcgacc tggcggtcgc
     1021 gccccacgat gtccggcagg tcgggtatgc caccgatggt caccgtgatg gggcggatcc
     1081 ggtctgcgct cggcgtcatc cgtcaacatt agacaccagg tctgatgtac gtcaagtctg
     1141 atgtgcatcc cgcctgatgc ccggtgagga tcccggcagt gatcgttgac agcgcgcata
     1201 ggctccctga ctaaagggtg ccaaacgcaa ggagtggccc atgccgaatg ccctgaccgg
     1261 cgacttcgac gccgtccttg agctcagtgg cggcacactc aatcgcctgg tcgcgacaat
     1321 gcatcagaac ggcgccgact ccgggaaacc gagcctgccg cacgttgcct acttccggct
     1381 cggcgacgac agggcctgac tgcctggacg gtgccggacc gatggcagag ccgcgggacg
     1441 acggtgacgg cggtcatgga ccgcgtcgcc cccgcgacgc acgcgcaaga ggacatcgga
     1501 gtaggcgggg gcggctgggt ccgcaagccg gacccgccca gccggggacc gcccagccgg
     1561 ggaccacatg ggcggggacc gcccagccgg ggaccacatg ggcgggaacg gcgtagattg
     1621 accgtacgtc tggataggca cgccccgcgg agtcgcgacg ggcgacgtga gccaaggagc
     1681 atgatgaccg accagccatc gaccccgtcg agcgatgccg cgatcgcccc gctcgagccg
     1741 ccctcgtcga ccctgacact gaccgctccg gcgcccccga aggctgtcgc cgagacgtca
     1801 gcgcccaaga tggccccggc cgtccccgag gccgcgctgc ccgggctgga ctcgaaggtc
     1861 gactcctacc tcggtcagct gctggcggcc ccggccaagt cgcccgagtt cgaggccaag
     1921 gcgaccgacg tgcgcacgat gggcgacgcc gacatacgcc gggcggcgga gagctccaac
     1981 cggctgctgc agtcaccggt caaggcgctg caagagggcg gcctgtccga agggtcgaag
     2041 gtgggtgcga ccttgctgga cctgcgccgc accgtcgagg acctcgaccc ggcgcaggcg
     2101 acgggggtca agaaggtcct ggggatgatc cccttcgggg acaaggtcac cgactacttc
     2161 cggcgctacc agtcggcgca gagccacctc aacggcatcc tgcacgccct gcgcgagggc
     2221 caggacgagc tgacgaagga caacaccgcc ctcaacctcg agaagcagca gctctgggac
     2281 acgatgggca tgctcaacca gtacgtgtat gtcgcggagc ggctcgactc gcgcctcgcc
     2341 gcgagcatcg ccaccctcga cgtctccgac ccggacaagg cccgctcgct gcgcgacgac
     2401 gtcatgttct acgtccgcca gaagcaccag gacctgctga cccagctggc cgtgtcgatc
     2461 cagaacttcc tcgccatcga catcgtcatc aagaacaacc tcgagctcat caagggggtc
     2521 gaccgggcat cgacgacgac gatctcggcc ctgcgcaccg cggtgatcgt ggcccaggcc
     2581 ctcggcaacc agaagctcgt cctcgaccag atcaccgccc tcaacgtgac gacctcgggc
     2641 ctgatcgagc ggacctcgga gatgctcaag gacaactcgg tcaagatcca ggagcaggcg
     2701 gcctcggcga ccatcgggct gccccagctg cagcgggcct tcgacaacgt ctaccagacg
     2761 atggacgcga tcgacgcgtt caaggtccag gccctggact cgatggcggc cacgatcggc
     2821 accctcgagg gcgaggtcgt caagtcccgc tcctacctcg accgggtgca gcgccgggac
     2881 cagcggatcg cctccggctc gctcgacatc acctcgctcg ggcgctgatg ggcattggtg
     2941 actggctcgg gtggggcaag gcggaaccgg cccctccggt tcacgccgtc ccgacgagcg
     3001 agcacctcat cgaggcgctg acccgcgtcg agaccctcgc ccgccagggc gccgtccccg
     3061 ggccggtcat gtcgcgcctg gcccgggtca cccgggtcgt ccgcgacacg atcccccggc
     3121 tcgacgcgct gggcgcgggc agcgtgtcgg catacaacgt catggccacg gccaccgact
     3181 acctgccgga ggcgatcggc ggctacctgc gcctgccgcg gcagtgggcc gactcgcgcc
     3241 cggtcgaccg cggcaagacc tcgctcatga tcctcatcga ccagctcgac ctgctcgggg
     3301 cgacgatgga caaggtcttc gacgccgtat gccggtccga cgccgaggcg ctggtcgtcc
     3361 acggccgctt cctgcaggag aagttcggct cgccctccgg cgggtcgctc gctctgcagc
     3421 aggtggcggc gcctcccgct cccgacccca ccagtaccag ccc
//
LOCUS       DATAIX010000726         5123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_261125, whole genome shotgun sequence.
ACCESSION   DATAIX010000726 DATAIX010000000
VERSION     DATAIX010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261125"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VHM65_10745"
     CDS             <1..618
                     /locus_tag="VHM65_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706228.1"
                     /translation="ASAKVVVLGGGVAGQNAANIALGMGADVTILDTDLDKLRTIFWR
                     WQNRIKQLASSTLAVRQQVLDADMVIGSVLIPGAKAPKLVTNEMVSAMKPGSVLVDIA
                     VDQGGCFEDTHPTTHDDPTFRVHGSTFYCVANMPGAVPNTSTYALTNATLPYALRLAR
                     LGWRDALRADPALALGLNTHGGSVTYQAVADDLSLAYQPLAGVLA"
     gene            664..1740
                     /gene="ord"
                     /locus_tag="VHM65_10750"
     CDS             664..1740
                     /gene="ord"
                     /locus_tag="VHM65_10750"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF040740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HEX2706229.1"
                     /translation="MSAQMTAQTTAQTTPIRVLQWGLGAMGAGMARLILDKPGLELVA
                     AVDARPDYVGADLGDVLGIGRRLGVTVTDHPEDVLDAASVDCVTIATTSWAAQQRPDL
                     RRILGAGINVVTIAEEMAEPAAQNPQLSDELDALAREHGVSVVGVGVNPGLVLDLLVV
                     ALTAGSHTVERIEASRVNDLSPYGPTVMESQGVGMSVEAFRAGVADGTIVGHVGFPES
                     IALISDALGFGIDRIEQTREPIVSTVRRETPHVVVAPGMVAGCAHTGIGYRGDTELVR
                     LVHPQQIHPHLEQQPTGDYIHIYGTPEIALSIQPEIAGGIATQGMAVNMIPVLVAATP
                     GLKRVIDLPVPAALMGPSAYRRRV"
     gene            1761..2045
                     /gene="ortA"
                     /locus_tag="VHM65_10755"
     CDS             1761..2045
                     /gene="ortA"
                     /locus_tag="VHM65_10755"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: protein motif:HMM:NF040739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HEX2706230.1"
                     /translation="MSSVAAGTWVEIEQVVLTPAERAPGLPEDTRSVPLLLRVSGFLL
                     GDASVGDEVRIRTIIGREHAGLLRVVSPGYSHSFGDVVPELLTIGTEAEQ"
     gene            2042..3523
                     /gene="ortB"
                     /locus_tag="VHM65_10760"
     CDS             2042..3523
                     /gene="ortB"
                     /locus_tag="VHM65_10760"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: protein motif:HMM:NF040741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HEX2706231.1"
                     /translation="MTARDTSYAAVMARRNEIMKASLGMDYDDYVSSPIAFDYDAMMT
                     DTGYTIADIAAIQRETKVGNTALFELRNLTAAVRRLAEPGKGATILLKDEAANASGSF
                     KARRASISAYEARKHGYAGMVTATSGNYGAAVASQAAQRALKCIVVQEVYDSHGVGQP
                     EIVEKGRACEAYGAEVLKLTVGPELFYVLLRTLEETGFFNASLYTPFGIRGVETLGAE
                     IGAEVQARYGRVPDAVVVTHAGGGNLTGTARGLLATGCDRTRVVACSVDLTGLHMASD
                     RDFNRKSFTTGHSGFGVPFATWPDRVDVPRNAARALRYMDDYHLVTQGEVFFITELLT
                     KIEGIERGPAGNTSLTAAVALARQMGQDEVVVVQETEYTGAGKHHNSQLSFARKRGIE
                     VRRGDPRDSVPGVSIIIPERLAQVGAMVQDVDALRRSYLKNAAGVLPVAQWSDQDVAF
                     LAADLKVGPAWVTAHAAAAWPDQLHARAAALRPGTPVERTPSP"
     gene            3520..3888
                     /gene="oraS"
                     /locus_tag="VHM65_10765"
     CDS             3520..3888
                     /gene="oraS"
                     /locus_tag="VHM65_10765"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF027866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraS"
                     /protein_id="HEX2706232.1"
                     /translation="MTDDRTARLEARVAELAGMPAEALKDMFWELNEAVVAPLVDLAR
                     THTTPSIERSVLLRMGVDSLTSHGVVDHIHEAGLLGKGAGQVVLRVAQRDGLGIREAA
                     AAIAGSTDVLTGLFPGGGSR"
     gene            3885..>5123
                     /locus_tag="VHM65_10770"
     CDS             3885..>5123
                     /locus_tag="VHM65_10770"
                     /inference="COORDINATES: protein motif:HMM:NF020610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HEX2706233.1"
                     /translation="MTGPEPLDPEVKLDIEAIVSDLEHYRPTRKGWTWRDVPEGGVDM
                     GPFHYRDMSTPLTRSIPLPAAKYFDNVDPQPSCVVTSEIASGRFEDDLRRMRMAAWHG
                     ADHLMVIRTTGQSHIDGLVEGTPEGVGGVPITRKQIRATRKALDLIEAEVGRPINFHS
                     YVSGVAGPEVAVLFAEEGVNGAHQDPQYNLLYRNINGHRSYVDAAEAKKVMAGAGIFQ
                     IDGAHNANATARAGWLVLPELMVQHGINCAFSVASGMSKDLIGLSTVPPNAPPAPKLW
                     YDLPYAVALRDFFADFKMRAQQNTRYIEADLAEAIRTHTVDTLISMLTRADVQSTITP
                     DEGRNVPWHYNNVRAIQTVKQTWAALDGITELLTLNHDGPLGEMVRDIKARAVVFLEE
                     ILATGGYFAAVEAGFFVDSAR"
BASE COUNT          813 a         1898 c         1717 g          695 t
ORIGIN      
        1 gccagcgcca aggtcgtcgt cctcggtggc ggggtggccg ggcagaacgc cgcgaacatc
       61 gcgctgggca tgggcgccga cgtgacgatc ctggacaccg acctggacaa gctccggacg
      121 atcttctggc gctggcagaa ccggatcaag cagctggcct cctcgaccct ggccgtgcgc
      181 cagcaggtcc tcgacgccga catggtcatc gggtcggtcc tgatccccgg cgccaaggcc
      241 cccaaactgg tgaccaacga gatggtctcg gccatgaagc ccggctcggt gctggtcgac
      301 atcgccgtcg accagggcgg ctgcttcgag gacacccacc cgaccaccca cgacgacccg
      361 accttccggg tgcacggctc gacgttctac tgcgtcgcca acatgcccgg cgcggtgccg
      421 aacacctcga cgtatgccct gaccaacgcc accctgccct acgccctgcg cctggcccgc
      481 ctgggctggc gcgacgcgct cagggccgac cccgcgctcg cactcggcct caacacccac
      541 ggcggctccg tcacatacca ggccgtcgcc gacgatctct ccctggccta ccagccgctg
      601 gccggcgtgc tggcctgacc gctggcaccc gacgtccgca ccgccgctcg acgaggagcg
      661 cagatgagcg cgcagatgac agcgcagacg acagcgcaga cgacaccgat tcgcgtcctg
      721 cagtgggggc tcggtgcgat gggggccggc atggcccggc tcatcctgga caagcccggg
      781 ctcgagctcg tcgcggccgt cgacgcgcga ccggactacg tcggcgccga cctcggagac
      841 gtcctgggga tcggtcgccg gctcggggtg acggtgaccg accaccccga ggatgtcctc
      901 gacgccgcct cggtcgactg cgtgacgatc gcgacgacat cctgggcggc ccagcagcgc
      961 cccgacctgc gccgcattct cggtgcgggc atcaacgtcg tgacgatcgc cgaggagatg
     1021 gccgagccgg ccgcccagaa cccgcagctc tcggacgagc tggacgccct ggcccgcgag
     1081 cacggcgtct ccgtcgtcgg cgtgggcgtc aacccagggc tggtcctcga cctgctcgtc
     1141 gtggccctca ccgcgggcag ccacacggtc gagaggatcg aggcgtcccg ggtcaacgac
     1201 ctgtccccct acggcccgac cgtcatggag agccagggtg tcggcatgag cgtcgaggcg
     1261 ttccgggccg gtgtggcgga cggcacgatc gtcggccacg tcggcttccc ggagtcgatc
     1321 gccctcatca gcgacgcgct ggggttcggc atcgaccgga tcgagcagac ccgcgagccc
     1381 atcgtctcca cggtgcgccg ggagaccccg cacgtcgtgg tggcccccgg catggtcgcc
     1441 gggtgcgccc acaccgggat cggctaccgg ggcgacaccg agctggtccg cctcgtccac
     1501 ccccagcaga tccacccgca cctggagcag cagccgacgg gcgactacat ccacatctac
     1561 gggacccccg agatcgcgct gagcatccag ccggagatcg ccggcgggat cgccacgcag
     1621 gggatggcgg tcaacatgat cccggtcctc gtggcggcca cgccgggcct caagcgggtc
     1681 atcgacctgc ccgtccccgc cgcgctcatg ggaccgagcg cctaccgtcg gcgggtctag
     1741 ccatcgagaa gggtgcaccg gtgagcagtg tcgccgcagg gacctgggtc gagatcgagc
     1801 aggtcgtcct cacccctgcc gagcgggcgc cgggcctgcc ggaggacacc aggtccgtcc
     1861 ccctgctcct gcgggtctcc ggcttcctgc tcggcgacgc ctcggtcggc gacgaggtga
     1921 gaatccgcac gatcatcggc cgcgagcacg ccggcctcct gcgcgtggtc agccccggct
     1981 actcgcacag cttcggtgac gtcgtgcccg agctgctcac catcgggacg gaggccgagc
     2041 agtgaccgcg cgcgacacgt catacgcggc cgtcatggca cgccggaacg agatcatgaa
     2101 ggcctccctg gggatggact acgacgacta cgtcagcagc ccgatcgcct tcgactacga
     2161 cgcgatgatg accgacacgg gctacacgat cgcggacatc gccgcgatcc agcgcgagac
     2221 caaggtcggc aacacggccc tgttcgagct gcgcaacctc accgcagcgg tccgcaggct
     2281 cgccgagcca ggcaagggag cgacgatcct gctcaaggac gaggccgcca acgcctcggg
     2341 cagcttcaag gccaggcggg cgtcgatcag cgcgtacgag gcccgcaagc acgggtatgc
     2401 cggcatggtc actgccacga gcggcaacta cggcgccgcg gtggcgtcgc aggccgccca
     2461 gcgggccctg aagtgcatcg tcgtccagga ggtctacgac agccacgggg tggggcagcc
     2521 ggagatcgtc gagaagggcc gggcgtgcga ggcctatggc gccgaggtgc tcaagctcac
     2581 ggtgggaccc gagctgttct acgtcctgct ccgcacgctc gaggagaccg ggttcttcaa
     2641 cgcgagcctc tacaccccgt tcggcatccg cggggtcgag accctcggcg ccgagatcgg
     2701 cgccgaggtg caggcgcgct acgggcgcgt tcccgacgcc gtcgtggtca cccacgcggg
     2761 tggcggcaac ctcaccggaa ccgcccgggg cctgctcgcc accggctgtg accggacccg
     2821 ggtcgtcgcc tgttcggtgg acctcacggg cctgcacatg gcctccgacc gggacttcaa
     2881 ccgcaagtca ttcacgaccg gccattcggg cttcggggtg cccttcgcga cctggcccga
     2941 ccgtgtcgac gtgccgcgca acgctgcccg ggcactgcgc tatatggacg actaccacct
     3001 ggtcacccag ggcgaggtct tcttcatcac cgagctgctg accaagatcg agggcatcga
     3061 gcgcgggccc gcgggcaaca cgagcctcac cgcagccgtc gccctggccc gccagatggg
     3121 ccaggacgag gtcgtcgtcg tccaggagac cgagtacacc ggggccggca agcaccacaa
     3181 cagccagctg tccttcgccc gcaagcgcgg gatcgaggtg cgccgcggcg accccaggga
     3241 cagcgtgccg ggggtgtcca tcatcatccc cgagcggctc gcacaggtcg gcgccatggt
     3301 gcaggacgtc gatgcgctgc gccgctccta cctcaagaat gccgcggggg tgctgccggt
     3361 ggcgcagtgg tccgaccagg acgtggcctt cctcgcggcg gacctcaagg tcggcccggc
     3421 gtgggtcacc gcccacgccg cggccgcctg gccggaccag ctgcacgccc gggccgcggc
     3481 gctgcggccc ggcacacccg tggaaaggac gccttcgcca tgaccgacga ccgcaccgca
     3541 cggctcgaag cccgcgtggc cgagctggcc gggatgcctg ccgaggcgct caaggacatg
     3601 ttctgggagc tcaacgaggc cgtcgtcgcg cctctcgtgg acctcgcgcg gacccacacg
     3661 accccgtcca tcgagcgctc ggtcctgctg cggatgggcg tggacagcct cacctcgcac
     3721 ggcgtcgtcg accacatcca tgaggccggt ctgctcggca agggggccgg ccaggtcgtg
     3781 ctgcgggtcg cccagcgcga cggcctgggc atccgggagg ctgccgcggc gatcgccggg
     3841 agcaccgacg tgctgaccgg gctcttcccc ggaggtgggt cacgatgacc ggtcccgaac
     3901 cgctcgaccc cgaggtcaag ctcgacatcg aggccatcgt cagcgacctg gagcactacc
     3961 ggccgacccg caaggggtgg acctggcggg acgtgcccga ggggggcgtc gacatgggtc
     4021 cgttccacta ccgggacatg tccacgccgc tgacccgcag catcccgctg ccggccgcga
     4081 agtacttcga caacgtcgac ccgcagccct cctgcgtcgt caccagcgag atcgcctcgg
     4141 gacggttcga ggacgacctg cgtcgcatgc ggatggccgc ctggcacggt gcggaccacc
     4201 tcatggtcat ccggacgacc gggcagagcc acatcgacgg cctcgtcgag ggcacgcccg
     4261 agggcgtcgg cggtgtcccg atcacccgca agcagatccg ggccacccgc aaggccctcg
     4321 acctcatcga ggccgaggtc ggccggccga tcaacttcca ctcgtacgtc tcgggcgtcg
     4381 cgggacccga ggtcgccgtc ctcttcgccg aggaaggggt caacggggca caccaggacc
     4441 cccagtacaa cctgctctac cgcaacatca acgggcaccg gtcctacgtc gacgcggcgg
     4501 aggccaagaa ggtcatggcc ggtgccggga tcttccagat cgacggtgcg cacaacgcca
     4561 acgcgacggc ccgggccggc tggctcgtgc tgcccgagct catggtccag cacggcatca
     4621 actgcgcctt ctcggttgcc tccggcatgt cgaaggacct catcggcctc tcgacggtcc
     4681 cccccaacgc gcctccggca ccgaagctct ggtacgacct gccgtatgcc gtcgccctgc
     4741 gcgacttctt cgccgacttc aagatgcggg cccagcagaa cacccgctac atcgaggccg
     4801 acctggccga ggcgatccgg acccacacgg tcgacacgct catctccatg ctgacccggg
     4861 ccgatgtcca gagcacgatc accccggacg agggccgcaa tgtcccgtgg cactacaaca
     4921 acgtccgggc catccagacg gtcaagcaga cctgggcggc gctcgacggg atcaccgagc
     4981 tgctcacgct caaccatgac ggccccctcg gcgagatggt ccgtgacatc aaggcccggg
     5041 ctgtcgtctt cctcgaggag atcctcgcca ccgggggcta cttcgcggcg gtcgaggccg
     5101 ggttcttcgt cgactccgcg agg
//
LOCUS       DATAIX010000727         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_261490, whole genome shotgun sequence.
ACCESSION   DATAIX010000727 DATAIX010000000
VERSION     DATAIX010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261490"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..>1523)
                     /locus_tag="VHM65_10775"
     CDS             complement(34..>1523)
                     /locus_tag="VHM65_10775"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF010707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEX2706234.1"
                     /translation="AARERLAALGFRDPVVALRHLEALTVGVSRRAAIQRTLLPVMLG
                     WFADEADPDAGLLAFRRVSDELGSIHWYLKMLRDEGSSAERLAHVLGRSRYVADLLVR
                     SPESVAFLGDATGLAPSSRADLTATMTSAAGRRHDPDLAIAAVQSVRRHELLRIAFGD
                     LVGGLSLREVGRALTDLTAATFEAALQIATRVVAAQHDGVLGTRMLVVGMGSLGGSEM
                     GYGSDADVLFVHEPEEGVEENEAQARALQAVQELIRLLGAAGSAPPLGVSAELRPEGR
                     NGPLVRSLESYRAYYERWALTWEFQALLRAAPVAGDLGLAGRFVALIEPVRWPEGGIS
                     ARQVRDIRTLKARVEAERLPRGADPRTHFKLGRGGLTDVEWTVQLLALRHGYAHPALR
                     TTSTMEALDAAESEGLITPGHARDLREGWTLAQSLRNAAVLWRGRSLDSLPTDLRDAD
                     GVGRIIGRPPGRGADLAETYLRVARRCRLAMDLNFYLPDTAGTVG"
BASE COUNT          215 a          554 c          567 g          187 t
ORIGIN      
        1 cgacggcatg gtcacaccgg agctgtcgga cacctacccg accgtacccg cagtgtctgg
       61 gaggtaaaag ttgagatcca tcgccaggcg gcagcgacgt gccacccgca ggtaggtctc
      121 ggcgaggtcg gcgccgcgcc cggggggccg gccgatgatg cgccccaccc cgtcggcgtc
      181 cctgaggtcg gtcgggagcg agtccagcga tcggccccgc cacagcaccg cggcgttgcg
      241 caacgactgg gccagggtcc acccctcgcg caggtccctg gcatgcccgg gcgtgatgag
      301 tccctccgac tcggcggcgt cgagggcctc catcgtgctc gtcgtccgca gtgccgggtg
      361 ggcgtacccg tggcgcaggg cgagcagctg gacggtccac tcgacatcgg tgagcccgcc
      421 gcgacccaac ttgaagtggg tgcgcgggtc ggctccccgc ggtagccgtt cggcctcgac
      481 ccgcgccttg agggtgcgga tgtcgcgcac ctggcgtgcg ctgatcccgc cctcgggcca
      541 gcgcaccggt tcgatgaggg cgacgaagcg gccggccagg cccaggtcac cggccacggg
      601 tgctgcccgc agcagcgcct ggaactccca ggtgagggcc caccgctcgt agtaggcccg
      661 gtaggactcc agcgagcgca ccagcgggcc gttcctgccc tcgggacgca gctcggcgct
      721 gacaccgagc ggcggagccg acccggcggc cccgagcagc cggatgagct cctggacggc
      781 ctgcagggcc cgggcctgcg cctcgttctc ctcgaccccc tcctccggct cgtggacgaa
      841 gagcacgtcg gcatcggagc cgtagcccat ctcggagccg ccgaggctgc ccatgccgac
      901 gacgagcatc cgggtgccga ggacgccgtc gtgctgggcg gcgacgacgc gggtcgcgat
      961 ctgcagtgct gcctcgaagg tcgcggcggt gagatcggtc agggcccggc cgacctcgcg
     1021 cagcgacagc ccgcccacga ggtcgccgaa ggcgatgcgg agcagctcgt ggcggcggac
     1081 cgactggacg gcggcgatgg ccaggtccgg gtcgtgcctg cggcccgcag cgctggtcat
     1141 cgtcgccgtg agatcggccc gggagctcgg ggcgagcccg gtcgcgtcgc cgaggaaggc
     1201 cacggactcg ggcgagcgca cgagcaggtc cgcgacatac cggctccgtc cgagaacgtg
     1261 cgcgagccgc tccgccgagc tgccctcgtc gcgcagcatc ttgaggtacc agtggatcga
     1321 gccgagctcg tcactgaccc ggcggaaggc gagcaggccc gcgtcagggt cggcctcgtc
     1381 ggcgaaccac ccgagcatga cgggcaggag cgtgcgctgg atagccgcac ggcggctcac
     1441 cccgaccgtg agcgcctcga ggtggcggag cgccacgacc gggtcgcgga agcccagcgc
     1501 agccagccgc tctcgcgccg cag
//
LOCUS       DATAIX010000728         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_261541, whole genome shotgun sequence.
ACCESSION   DATAIX010000728 DATAIX010000000
VERSION     DATAIX010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261541"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VHM65_10780"
     CDS             <1..447
                     /locus_tag="VHM65_10780"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX2706235.1"
                     /translation="AQVTSPGRLEVVRRSPTILVDAAHNPHGARALRAALADEFTFTT
                     LVGVLAVFADKDAAGMIEELEPVLDAVVVTRTASARAMRPADLGEIAVEYFGEGRVRV
                     VSELPDAIEAAVALAEADGMGGGVIATGSVFTAAEVRLLLGAGPGS"
     gene            444..809
                     /locus_tag="VHM65_10785"
     CDS             444..809
                     /locus_tag="VHM65_10785"
                     /inference="COORDINATES: protein motif:HMM:NF025383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4233 domain-containing protein"
                     /protein_id="HEX2706236.1"
                     /translation="MTGLLYGTLGKFTWRMCATVLAGQSIVVFFGALVARAVGATRAD
                     TGATSYLVVGSALAVLCLLGAGLMRRPYGVTLGWALQAATFASAVVVPAMVVVGLIFT
                     ALWVLCLVQGRRLDSLPSV"
     gene            850..1284
                     /gene="ndk"
                     /locus_tag="VHM65_10790"
     CDS             850..1284
                     /gene="ndk"
                     /locus_tag="VHM65_10790"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX2706237.1"
                     /translation="MTTSALQRSLVLVKPDAFARGLTGEVLRRIEAKGYRLVALRILT
                     PTDEMLGRHYAEHVDKPFFPELCTFMSSGPVTAVVLEGEQCIAGFRSLAGVTDPTKAA
                     PGSIRGDLGRDWGMGTTENVVHGSDSPESAAREIALWFPDLG"
     gene            1427..>1929
                     /locus_tag="VHM65_10795"
     CDS             1427..>1929
                     /locus_tag="VHM65_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX2706238.1"
                     /translation="MLDVAALAGVSLKTVSRVVNREPGVSQTLRERVDRAASGLDYRH
                     NLAASNLRRTQSRASLIGVLLQDVGNSFSSALLRAIEDEARLRGVVVVAASLDEEPER
                     EVALVSDLVARRIDGLILMPATHRQDYLVSEARAGLPTVFVDRRPAGVDTDSVVVDNW
                     GGAWQAT"
BASE COUNT          236 a          667 c          703 g          323 t
ORIGIN      
        1 gcccaggtga cgtcgcccgg gcggctcgag gtggtccgac gctcgccgac gatcctcgtc
       61 gacgccgcgc acaacccgca tggcgcccgg gctctgcggg ccgcgctcgc cgacgagttc
      121 accttcacga cgcttgtcgg agtcctcgcg gtgttcgctg acaaggacgc cgccgggatg
      181 atcgaggagc tcgaaccggt gctcgacgca gtcgtcgtca cccggactgc ctctgcgcgg
      241 gccatgcgtc ccgcggacct gggcgagatc gccgtcgagt acttcggcga gggccgggtc
      301 agggtcgtgt ccgagctgcc cgacgcgatc gaggcggccg tcgcgctcgc cgaggccgac
      361 gggatgggcg gtggcgtcat tgcgaccggc tcggtcttca ccgccgccga ggtccgcctg
      421 ctgctcggag cgggacccgg atcatgaccg gcctgctcta cggcaccctg ggcaagttca
      481 cctggcggat gtgcgccacc gtgctcgcgg gccagtcgat cgtcgtgttc ttcggcgccc
      541 tcgtcgcccg ggcggtcggc gcaacgcgcg cggataccgg cgcgacgtca tacctcgtcg
      601 tgggctctgc cttggccgtg ctctgcctgc tgggcgcggg cctcatgcgc cgcccgtatg
      661 gcgtcaccct cggctgggcg ctgcaggcgg cgacgttcgc ctcggccgtc gtcgtgccgg
      721 ccatggtggt ggtcgggctg atcttcaccg cgctgtgggt gctctgcctg gtccaggggc
      781 ggcggctcga ctccctcccc tccgtgtgac gtggtggacg ctgacgttgc gctgggtagg
      841 ctgggccgca tgaccacctc cgcgttgcag cgttcactcg tcctggtcaa gcccgacgcg
      901 tttgcccggg ggctcacggg ggaggtgctg cgccggatcg aggccaaggg ctaccgactc
      961 gtggcgctgc gcatcctgac gccgaccgat gagatgctgg gccggcatta cgccgagcat
     1021 gtcgacaagc cgttcttccc tgagctgtgc accttcatgt cttccggacc cgtgaccgcc
     1081 gtcgtgctcg agggtgagca gtgcattgcc gggttccgtt ccctcgccgg ggtgaccgac
     1141 ccgaccaagg ccgcgccggg ctcgatccgc ggtgacctcg gccgggactg gggcatgggg
     1201 acgaccgaga acgtcgtgca cggctcggac tccccggagt cggccgcccg ggagattgcc
     1261 ctgtggttcc cggacctcgg ctgacggttc ggcgaccaag ggcaggtccg gctcgggtcg
     1321 gggccgaggc ttgccagtcc gggtgccgtg gggtagaacg gtccccatgg tggtgacaac
     1381 gatgtcttcg ggcgctcccc ggcgtggtgg caaaccacgg ccgacgatgc tcgacgtcgc
     1441 tgccctcgcc ggtgtcagcc tcaagaccgt ctcgcgggtc gtcaaccgcg agccgggcgt
     1501 ctcccagacg ctgcgcgagc gggtcgaccg tgcggcgagc gggctggact accgccacaa
     1561 ccttgccgca agcaacctgc gccgaaccca gagccgggcc tcgctgatcg gggtgctgct
     1621 acaggacgtg ggcaacagtt tctcctcggc actgctgaga gcgatcgagg acgaggcacg
     1681 gctgcggggt gttgtggttg tcgcagcgag tctcgacgag gagcccgagc gagaggtggc
     1741 gctggtgtcc gacctcgtgg cccggcgcat cgacggcctc atcctcatgc ccgcgacgca
     1801 ccgccaggac tacctcgtgt cggaggcgcg ggccggtctg cccacggtct tcgtcgaccg
     1861 ccgccctgcc ggagtggaca ccgactcggt tgtcgtcgac aactggggcg gggcctggca
     1921 ggcgaccga
//
LOCUS       DATAIX010000729         2745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_261749, whole genome shotgun sequence.
ACCESSION   DATAIX010000729 DATAIX010000000
VERSION     DATAIX010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261749"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VHM65_10800"
     CDS             <1..770
                     /locus_tag="VHM65_10800"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX2706239.1"
                     /translation="GEAAEAVRRVLGHTPNLALLGLHSHIGSQIFDTSGFEISARRVL
                     GLHAEVARDHGIELPELDLGGGFGIAYTTEHDPLTPKELGASMADFVERECRAGGVAV
                     PRISIEPGRAIAGPSTFTLYEVGTVKPVQLDGGATRVYVSVDGGMSDNIRTALYDADY
                     SCTVANRTSGATSVLGRVVGKHCESGDIIVKDEFVPADIAPGDLLAVPGTGAYCRALS
                     SQYNHTPRPPVVAVRDGEARLIVRRETIEDLLGLDVG"
     gene            809..1852
                     /locus_tag="VHM65_10805"
     CDS             809..1852
                     /locus_tag="VHM65_10805"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX2706240.1"
                     /translation="MVTENRARQEPLRVALLGCGVVGSAVARLLTEHAEDLAARIGRP
                     VQIVGIAVRRPGRDRSDTGVDASLFTTDAADLVTRADIVIEVIGGIEPARSLILAAMA
                     RGASVVTANKALLAEDGPTLYDAAERYGVDLYYEAAVAGAIPLLRPLRESLAGDAVRT
                     VLGIVNGTTNYVLDKMDTTGAGFTEAVEQAQALGYAEADPTADVEGFDAAGQLMFYGR
                     GAGGDPTASAVLGDLVAVARHKVTGGRGPGESTYADLPVMTMGQAYTRYHISLDVADR
                     PGVLAQVATAFADNNVSIETVRQRAALGGGGAELVIVTHRAPDAALSATVDALAGLPV
                     VAGVTSVMRVEGS"
     gene            1854..>2745
                     /gene="thrC"
                     /locus_tag="VHM65_10810"
     CDS             1854..>2745
                     /gene="thrC"
                     /locus_tag="VHM65_10810"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX2706241.1"
                     /translation="MAHQWRGVIAEYADRLPSLAGAPVVTLLEGGTPLIPAAHLSGLV
                     GAQVWLKYEGLNPTGSFKDRGMTTAISVAARLGARAVICASTGNTSASAAAYAAKAGM
                     ACGVLVPDGKISMGKLSQAIAHGATLLQVDGNFDDCLNLARKLAEAYPVELVNSVNPA
                     RIEGQKTASFEIVDALGDAPAIHCLPVGNAGNITAYWRGYREYHAGSDTPGPATHLPQ
                     MWGFQAAGSAPIVAGHPIDHPETIATAIRIGNPASWEQAVAARDDSAGRIEAVTDEQI
                     LAAHRILSAREGVFVEPASAA"
BASE COUNT          402 a         1008 c          956 g          379 t
ORIGIN      
        1 gcggcgaggc ggccgaggcg gtgcgccgcg tcctcggcca cacgcccaac ctggcgctgc
       61 tcggtctgca cagccacatc ggttcgcaga tcttcgacac ctccggcttc gagatctcgg
      121 cccgacgggt cctggggctg cacgccgagg tggcccgcga ccacgggatc gagctgcccg
      181 agctcgacct cggcggcggg ttcggcatcg cctacacgac cgagcacgac ccgctcacgc
      241 ccaaggagct cggggcgagc atggccgact tcgtcgaacg ggagtgccgg gcgggcggcg
      301 tggcagtccc tcggatctcg atcgagccgg ggcgggccat cgcggggccg agcacgttca
      361 cgctctacga ggtggggacc gtcaagccgg tccagctcga cggcggcgcg acacgggtct
      421 acgtatccgt cgacggcggg atgagcgaca acatccggac tgccctgtat gacgcggact
      481 actcgtgcac ggtggccaac cggacgagcg gggctacttc cgtgctcggc cgggtcgtcg
      541 gcaaacactg cgagagcggg gacatcatcg tcaaggacga gttcgtgccc gcggacatcg
      601 cacccggcga ccttctcgcc gtcccgggca cgggtgccta ctgccgggcc ctgtcgagtc
      661 agtacaacca cacgccccgg ccgccggtcg tggccgtccg cgacggcgag gcgcgcctca
      721 tcgtgcgtcg tgagacgatc gaggaccttc tcgggctcga cgtcggctga ggctcggcca
      781 gagagtggac caacggcagg attcatacgt ggtgaccgag aaccgggcgc ggcaggagcc
      841 cctgcgcgtc gcgctgcttg ggtgcggggt cgtcggctcg gcggtcgcgc gcctgcttac
      901 cgagcatgcc gaggacctgg cggcgcgcat cgggcgcccc gtgcagatcg tcggcatcgc
      961 cgtgcgccgt ccggggcgcg accgctccga cacgggcgtc gacgccagcc tgttcaccac
     1021 ggacgctgcc gacctggtga cccgggccga catcgtcatc gaggtcatcg ggggcatcga
     1081 gccggcccgc agcctcatcc tggccgcgat ggcccggggt gcctcggtcg tcacggccaa
     1141 caaggccttg ctcgcggagg acggcccgac gctgtatgac gcggccgaac ggtacggcgt
     1201 cgacctctac tacgaagccg ccgtcgccgg cgcgatcccg ctgctgcgcc cgttgcgcga
     1261 gtccctggcc ggtgacgcgg tacgcacggt cctcggcatc gtcaacggca cgaccaacta
     1321 cgtcctggac aagatggaca cgaccggcgc cgggttcacc gaggcggtcg aacaggccca
     1381 ggcgctcggc tacgccgagg ccgacccgac cgccgacgtc gagggcttcg acgccgccgg
     1441 gcagctcatg ttctacgggc gcggcgcggg cggcgacccc accgcgtcgg ctgtcctcgg
     1501 cgacctcgtc gccgtcgccc ggcacaaggt gacaggcggg cgtggacccg gcgagtcgac
     1561 ctacgccgac cttcccgtca tgacgatggg gcaggcctac acgcgctacc acatcagtct
     1621 cgacgtcgcc gaccggccgg gcgtactcgc ccaggtcgca acggctttcg ccgacaacaa
     1681 cgtctccatc gagacggtcc gccaacgcgc cgccctcggc ggcggcgggg ccgagctcgt
     1741 catcgtgacc caccgggcgc cggatgcggc gctctcggca acggtggacg ccctcgccgg
     1801 gcttccggtc gtcgccggcg tcacctcggt catgcgggtg gaagggagct gacatggcgc
     1861 accaatggcg cggggtcatc gcggagtatg ccgaccgcct gccgtcgctc gccggtgccc
     1921 cggtggtcac ccttctcgaa ggcggcaccc cgctcatccc ggcggcgcac ctgtccgggc
     1981 tcgtcggcgc gcaggtctgg ctcaagtacg agggcctgaa cccgactggc tcgttcaagg
     2041 accgcgggat gacgaccgcg atctcggtgg cggcccggct cggggcccgc gccgtgatct
     2101 gtgcctcgac gggcaacacg agtgccagcg ctgcggcata cgcggccaaa gcaggtatgg
     2161 cgtgtggcgt gctcgtcccc gacggcaaga tctcgatggg caagctgtcg caggccattg
     2221 cccacggcgc gaccctgctc caggtcgacg ggaacttcga cgactgcctc aacctcgccc
     2281 gcaagctggc cgaggcgtac ccggtcgagc tggtcaactc ggtcaacccg gcgcgcatcg
     2341 agggccagaa gaccgcgtcg ttcgagatcg tcgacgcact cggcgacgcg cccgctatcc
     2401 actgcctgcc ggtcggcaac gccggcaaca tcacggccta ctggcggggc taccgcgagt
     2461 accatgccgg ctccgacacg cccgggccgg ccacacacct cccccagatg tggggtttcc
     2521 aggccgcagg ctcggcgccg atcgtcgccg ggcacccgat cgaccacccc gagaccattg
     2581 ccacggcgat ccggatcggc aacccggcct catgggagca ggcggtcgcg gctcgggacg
     2641 actccgctgg gcggatcgag gccgtcaccg acgagcagat cctcgccgcc caccgcattc
     2701 tctcggcccg cgaaggggtc ttcgtcgagc ccgcctcggc cgcat
//
LOCUS       DATAIX010000730         1956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_143276, whole genome shotgun sequence.
ACCESSION   DATAIX010000730 DATAIX010000000
VERSION     DATAIX010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143276"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VHM65_10815"
     CDS             complement(<1..375)
                     /locus_tag="VHM65_10815"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX2706242.1"
                     /translation="MTTPTGDPRVADVRARLSAAYQASAGPSDGARAKLDSQNKLYVR
                     DRIALLFDEGSFVEDGTFANVTAGDLPADGVVTGAATIDGRPVALMANDSTVKAGSWG
                     ARTVEKIIRIIETAYNSGVPMVY"
     gene            complement(430..1614)
                     /locus_tag="VHM65_10820"
     CDS             complement(430..1614)
                     /locus_tag="VHM65_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2706243.1"
                     /translation="MFELGQDYEMFRRVVRDFAASEVAPHVAQWDRDSYFPVELMPKL
                     GELGLFGLVVPEEYGGADPGEGAFTYLCVAIEELGRVDQSIGITLSAGVGLGINPILT
                     FGTHEQKERWLPDLVAGRTLAAFGLTEPDAGSDAGATATTAVLDGDEWVVNGSKAFIT
                     NAGTSITSVVTVTARTGTRPNGSAEISAIIVPNGTPGYTVDTAYHKLGWHISDTHGLH
                     FADARVPQENMLGERGHGFRQFLATLDDGRIAISALAVGCIQACLELATEYAKTRIAF
                     GRPIGANQGVSFQIADLAVMAEAARTLTYKAAWLKDEYTAGRRNLEDVKRAASIAKLY
                     STEAAVVATGIATQIHGGNGFMEEYPVARFYRDAKILAIGEGTSEIQRMVLARLLGLP
                     SD"
     gene            1699..>1956
                     /locus_tag="VHM65_10825"
     CDS             1699..>1956
                     /locus_tag="VHM65_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2706244.1"
                     /translation="MTSGAEVRQRTAGAAPGPDAAPSPTWTIAQVADEFGITHRTVRH
                     YEELGLLTPERRGTTRVYHRRDRTRLALILRGKRLGFPLEQV"
BASE COUNT          278 a          664 c          703 g          311 t
ORIGIN      
        1 gtagaccatc gggacgccgg agttgtaggc ggtctcgatg atgcggatga tcttctcgac
       61 cgtgcgggct ccccaggagc cggccttcac ggtggagtcg ttcgccatca gggcgaccgg
      121 tcgcccgtcg atggtggccg cgccggtgac cacgccgtcg gcgggcaggt cacccgccgt
      181 gacgttggcg aacgtcccgt cctcgacgaa ggacccctcg tcgaagagca gcgcgatgcg
      241 gtccctgacg tagagcttgt tctggctgtc cagcttggcc cgggcgccgt cggagggacc
      301 ggccgaggcc tggtatgccg cactcaagcg ggcgcggacg tccgcgaccc gagggtcgcc
      361 ggtgggggtc gtcatactgg ccatccttgt ccttgtccgg gctgcccgct caccagcaca
      421 gctggccggc tagtcgctcg gcaggccgag caggcgggcg aggaccatcc gctggatctc
      481 cgaggtgcct tcaccgatcg cgaggatctt ggcgtcccgg tagaagcggg cgacgggata
      541 ctcctccatg aagccgttgc caccgtggat ctgggtggcg atgcccgtcg caaccaccgc
      601 ggcctcggtc gagtagagct tggcgatcga ggcagcccgc ttgacgtcct cgaggttgcg
      661 ccgcccggcg gtgtactcgt ccttgagcca ggccgccttg taggtcaggg tccgcgcggc
      721 ctcggccatc acggcgaggt cggcgatctg gaagctcacg ccctggttgg ccccgatcgg
      781 gcggccgaag gcgatccggg tcttggcgta ctcggtcgcg agctccaggc acgcctggat
      841 gcagccgacc gcgagggcgc tgatcgcgat gcgcccgtcg tcgagggtgg cgaggaactg
      901 gcggaagccg tggccgcgct cgccgagcat gttctcctgc ggcaccctgg cgtcggcgaa
      961 gtgcaggccg tgggtgtccg agatgtgcca gccgagcttg tggtaggccg tgtcgaccgt
     1021 gtatcccggc gtgccgttgg gcacgatgat cgcgctgatc tcggccgagc cgttggggcg
     1081 ggtgccggtg cgggcggtca ccgtgacgac gctcgtgatc gaggtgccgg cgttggtgat
     1141 gaaggccttg ctgccgttga cgacccattc gtcaccgtcg agcacggcgg tcgtcgcggt
     1201 ggccccggcg tcggaccccg cgtcgggctc ggtgaggccg aaagcggcga gcgtgcgacc
     1261 ggcgacgagg tcgggcagcc agcgctcctt ctgctcgtgg gtgccgaagg tgaggatcgg
     1321 gttgatgccg aggcccacgc cggccgagag cgtgatgccg atcgactggt cgacccgccc
     1381 gagctcctcg atcgccacgc acaggtaggt gaaggccccc tctcccgggt cggcgccgcc
     1441 atactcctcc ggaacgacaa gcccgaagag cccgagctcg ccgagcttgg gcatgagctc
     1501 gaccgggaag taggagtcgc ggtcccactg ggccacatgg ggcgccacct cgctcgccgc
     1561 gaagtcgcgc acgacccgcc ggaacatctc gtagtcctgg cccagctcga acatggcctc
     1621 gtcctttcgc caccgcggga tgcccgcgtg gatggtttgc gcttgacgtt aacgtcgacg
     1681 taaagcacga tcgggactat gacaagtggc gcagaagtga ggcagcgcac tgcgggagcg
     1741 gctccgggtc cggacgcggc accctccccc acgtggacca tcgcccaggt cgccgacgag
     1801 ttcggcatca cccaccgcac ggtccgccac tacgaggagc tcggcctgct cacgcccgag
     1861 cgacgcggca cgacccgggt ctaccaccgc cgcgaccgga cacgcctcgc gctgatcctg
     1921 cgcggcaagc gcctcggctt cccgctcgag caggtc
//
LOCUS       DATAIX010000731         2484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_262618, whole genome shotgun sequence.
ACCESSION   DATAIX010000731 DATAIX010000000
VERSION     DATAIX010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262618"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1669)
                     /locus_tag="VHM65_10830"
     CDS             complement(77..1669)
                     /locus_tag="VHM65_10830"
                     /inference="COORDINATES: protein motif:HMM:NF020610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HEX2706245.1"
                     /translation="MPERRLLDLDPETVRTARRLAAKAGEPVVKLSKSHTTVSVERAT
                     LRLGGLAGADHEQVPWVNHLVDAVRASVGLEHGVSMPVFDAIRRGEAPDLLTLAQKAS
                     SGSVSFRLPDKAERGAAQRMARREVSRGIRKIDRQRATRDELIARIGDPKTKPWIYLI
                     VATGDIYEDIPQAQAAAREGADVIAVIRSTGQSLLDFVPEGATREGFAGTYATQENFK
                     LMRAALDEVGAEIGRYIRLTNYASGLCMPEIAALAGMERLDMMLNDSMYGILFRDINP
                     IRTFVDQRFSRQVHARAGIIINTGEDNYLTTADAVDAAHTVTVSQLLYEYFAKVAGLR
                     SWQLGLGHAFEVNPDLPDSFRIELAHALLARELFPKAPLKWMPPTKHMTGDIFRGYLL
                     DGFFNLAGALTNQSILLVGMMTEAVVTPFLSDRDLALQNVRYVLNAAGSLSEDFHPPK
                     NGFIQTRARLVLDEAVDLLDAIVYDRDEQGLPPLLTAIADGTFGLMKRPAGGGKGLDG
                     VIARAPGYDNPAITLLETGADR"
     gene            complement(1749..>2484)
                     /locus_tag="VHM65_10835"
     CDS             complement(1749..>2484)
                     /locus_tag="VHM65_10835"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX2706246.1"
                     /translation="LAKVGWNSLYWDNHDQPRALSRWGDDAAYRVESAKTLLTTLHLH
                     RGTPYVYQGDEIGMTNAPFTSIEDIADIESLRRYAEMAAAGADLDVVMDAVRARGRDN
                     ARTPMQWDGGPGAGFSTGTPWLAVNPNHVEVNAADAVADPGSVWHHVRQLIALRHTDP
                     VVAEGDYTQLVPEHDQAFVFTRRLGQGELLVVANWSSRELDLRSPAGPAGLKEWATAP
                     VVLATHPIGPTSSWLLAPWESRVYRR"
BASE COUNT          358 a          833 c          884 g          409 t
ORIGIN      
        1 ttgccgggcg cctccgagct cgggtcgccg gggacggcgc gctccatgtg ctgctcgaag
       61 gactgcttga cgctgctcat cggtccgccc ccgtctcgag caaggtgatc gccgggttgt
      121 cgtagccggg tgcgcgggcg atgacgccgt ccaggccctt cccgcctccc gccggccgct
      181 tcatgaggcc gaaggtgccg tcggcgatgg ccgtgagcag cggtggcagc ccctgctcgt
      241 cccggtcgta gacgatcgcg tcgagcaggt cgaccgcctc gtcgagcacg agccgggccc
      301 gggtctggat gaagccgttc ttcggcgggt ggaagtcctc ggacagcgag ccggccgcgt
      361 tgaggacgta gcgcacgttc tgcagcgcga ggtcccggtc ggacaggaac ggggtcacca
      421 cggcctcggt catcatgccg acgagcagga tgctctggtt ggtcagggca ccggccaggt
      481 tgaagaaccc gtcgagcagg tagccgcgga agatgtcgcc ggtcatgtgc ttggtcggcg
      541 gcatccattt gagcggcgcc ttggggaaca gctctcgggc gagcagcgcg tgggcgagct
      601 cgatgcggaa cgagtcgggc aggtcggggt tgacctcgaa ggcgtgcccg agcccgagct
      661 gccagctgcg cagaccggct accttggcga agtactcgta gaggagctgg ctcaccgtca
      721 ccgtgtgggc cgcgtcgacc gcgtcggccg tggtgaggta gttgtcctcg ccggtgttga
      781 tgatgatccc ggcgcgggcg tggacctggc gggagaaccg ctggtcgacg aaggtccgga
      841 tcgggttgat gtcgcggaag aggatgccgt acatcgagtc gttgagcatc atgtcgagcc
      901 gctccatccc cgcgagcgcc gcgatctccg gcatacacag cccggaggcg tagttcgtga
      961 gccggatgta gcgcccgatc tcggccccga cctcgtccag cgccgcccgc atgagcttga
     1021 agttctcctg ggtggcatag gtgcccgcga agccctcgcg ggtcgccccc tcgggcacga
     1081 agtcgagcag cgactggccc gtcgagcgga tgaccgcgat gacgtcggcg ccctcgcggg
     1141 ctgcggcctg ggcctggggg atgtcctcgt agatgtcgcc ggtcgcgacg atgaggtaga
     1201 tccacggctt cgtcttgggg tcgccgatcc gggcgatgag ctcgtcgcgg gtggcgcgct
     1261 ggcggtcgat cttgcggatc ccgcgcgaga cctcacgccg cgccatccgt tgtgctgcgc
     1321 cccgctcggc cttgtcaggc aggcggaagc tcaccgaccc gctcgaggcc ttctgggcga
     1381 gggtgagcag atcgggggcc tcaccccgac ggatcgcgtc gaacaccggc atgctcacac
     1441 catgctccag gcccaccgag gcgcgcacgg catcgacgag atggttgacc cacgggacct
     1501 gctcgtggtc ggccccggcg agcccgccca gccgaagcgt cgcacgctcg acggacaccg
     1561 tcgtgtggct cttggacagc ttgaccacgg gttcgccggc cttggccgcc aaccgccgtg
     1621 ccgtgcggac cgtttccgga tccagatcga gaagacgacg ctcaggcatt gtcccagaat
     1681 accagttgaa ccggcgattt tgcggtgagt ctcggctgtg gccgcaagat catcggtata
     1741 ggaccgtgtc atcggcggta caccctcgac tcccagggcg ccaggagcca ggaggacgtc
     1801 gggccgatcg ggtgggtcgc cagcaccacc ggtgccgtcg cccactcctt cagacccgcc
     1861 ggccccgcgg gtgagcgcag gtcgagctcg cgcgaggacc agttcgcgac gacgagcagc
     1921 tccccctggc ccaggcgacg ggtgaagacg aacgcctggt cgtgctccgg caccagctgc
     1981 gtgtagtcac cctcggccac caccgggtcg gtatggcgca acgcgatgag ctggcgcacg
     2041 tggtgccaga ccgaaccggg gtcggccacg gcgtccgcgg cgttgacctc gacatggttg
     2101 gggttgaccg ccagccacgg tgtgccggtg ctgaaccctg caccgggccc gccatcccac
     2161 tgcatgggcg ttcgcgcgtt gtcgcgcccc cgcgcccgca cggcgtccat caccacgtcg
     2221 aggtcggccc ctgcggcggc catctcggcg taacggcgca gcgactcgat gtcggcaatg
     2281 tcctcgatac tcgtgaacgg ggcgttcgtc ataccgatct cgtcgccttg gtagacatag
     2341 ggtgtcccgc ggtgcaggtg cagcgtcgtg agcagcgtct tggccgactc cacccggtat
     2401 gccgcgtcgt ccccccagcg ggacagggcc ctcggctggt cgtggttgtc ccagtagagg
     2461 ctgttccagc cgaccttcgc gagc
//
LOCUS       DATAIX010000732         4043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_143330, whole genome shotgun sequence.
ACCESSION   DATAIX010000732 DATAIX010000000
VERSION     DATAIX010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143330"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..1754
                     /gene="mtrB"
                     /locus_tag="VHM65_10840"
     CDS             48..1754
                     /gene="mtrB"
                     /locus_tag="VHM65_10840"
                     /inference="COORDINATES: protein motif:HMM:NF040691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system histidine kinase MtrB"
                     /protein_id="HEX2706247.1"
                     /translation="MSSTDSGVPAHPVGPRRFGAQVSTTARRLRSGLRRRWRSSLAFR
                     VIGVTILLGFAVLLAGGTYLYRQVADGLYEDRQRIAMSESLRLAKEAQSQFDTTDKTG
                     SLGEIEQFAQQVVGGLAGSEGTRYIILARAVTNENPQAISTVLSGPVGLASIPAALRE
                     AVAANGGTQQLQAISVTRAGAEDPVPAIVVGQEIQPPVVGAYGLYFIYPLDREQATLS
                     LVARTFGLGGLALILLVGAVAFVVTRLVVVPVREAAAVAVGFAEGHLNDRMHVKGEDD
                     LARLATSFNEMAASLQQQIAQLENLSRVQQRFVSDVSHELRTPLTTIRMAGDLIHDAR
                     ADFEPAVARSAELLHTQIDRFEALLADLLEISRFDAGAAALDIESNDLRGIVAQVIEV
                     CQPLADRRGSVVSLRGARRPCIADVDLRRVERVLRNLLVNAIEHGDGGPIEVRLGQSE
                     TAVAVSVRDVGVGLKPGERELVFTRFWRADPARARTTGGTGLGLAISLEDARLHGGSL
                     KAWGELGEGSCFRLTLPKTASHPAGRSPLPLGPVPRKSAPPATVVAGAEAASAGDAGA
                     GS"
     gene            1751..3565
                     /locus_tag="VHM65_10845"
     CDS             1751..3565
                     /locus_tag="VHM65_10845"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HEX2706248.1"
                     /translation="MRPRPRLGRRRGRALLVAAVLGGLCACGGLPTSGPVVSGRQVGA
                     GVLQPIRIEPIGPIPGADAGSVATGFVQAGAGFQDNTDGPDVARDFLTPRAAAGWKPT
                     SSVTVIEAPVSVGSADGSHVQVRAKAVARIDSAGHYTEVRPGTLVTAVFAIERLAGEW
                     RLDLPEGGFGLWLTSQDLQRLFEPVRIYYVTLSGRELVPETRWFPSGARLATTLARAQ
                     AEPVPAYLRGALTTGFPEATSLQVDAVPVVGGRANVTLTTAALQADPEQRRMMYAQMV
                     TALSQVLTVQEVGLLAGDTKLELSGLGDSARSFAELGYRIVGPASPRTGLLRQGSTLS
                     SVNLQQLAGLEQDTRPRAVTAPAATWPAIAPGWTSLALSVDGTELAGVGGDLKELRRW
                     RGTAEIPMGPFASSLTRPSYDAHGFLWTSGVSGGTTRAYVIDTAARGGAAPAAVKAAW
                     LAPRRVAALRVSPDGTRAAVVSRAPGASTDSLDIAGVIRTLAGQPVGLSAPGPEGQPL
                     SEVRDVTWLDIDTLGVLGRVSTIEPWRPYVVDLGQGIGLRSPGSALLSPVPDGQAITS
                     AGGPRGVLVRTERGRVKVRAGSSWVQLSAGSEVVVPGA"
     gene            3806..>4043
                     /locus_tag="VHM65_10850"
     CDS             3806..>4043
                     /locus_tag="VHM65_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706249.1"
                     /translation="MAFGSEHLRDGPCAVVPSPEPPGLPPVLSTYRYGGAVTIALRAY
                     KDAGRRDLGPVLAPALAPALTLALRVAARDPSARG"
BASE COUNT          483 a         1536 c         1444 g          580 t
ORIGIN      
        1 ccgtccgagg ggtcggctac aaggccggcc ccacctaggc ctgggccatg agttcgaccg
       61 actcgggggt gccggcccac ccggtcggac cgcggcgctt cggcgcccag gtcagcacca
      121 cggcgcggcg gctccggtcc ggcttgcggc gccggtggcg ctcctcgctc gcgttccggg
      181 tcatcggcgt gacgatcctg ctcgggtttg ccgtcctgct cgccgggggg acctacctct
      241 accgccaggt cgccgacggc ctgtacgaag accgccagcg aatcgctatg tccgagtccc
      301 tgcggctggc caaggaagcc cagtcccagt tcgacacgac cgacaagacc ggctccctgg
      361 gcgagatcga gcagttcgcc cagcaggtcg tcgggggtct cgcgggcagc gagggtaccc
      421 gctacatcat cctcgcccgc gcggtcacca acgagaaccc acaggccatc tcgaccgtgc
      481 tgtccggacc ggtggggctg gcgagcatcc ctgcggccct gcgtgaggcg gtcgccgcga
      541 acgggggaac ccagcagctg caggcgatct cggtcacccg ggccggggcc gaggaccccg
      601 tgcccgccat cgtcgtcggg caggagatcc agccgcccgt tgtcggggcg tacgggctgt
      661 acttcatcta cccgctcgac cgggagcagg ccacgctgag cctcgtcgcg cggaccttcg
      721 gactcggcgg gctcgcgctg atcctgctcg tcggggccgt cgcgttcgtc gtcacgcgtc
      781 tcgtcgtcgt ccccgtccgc gaggcggcgg ccgtcgccgt cggcttcgcc gagggccacc
      841 tcaacgaccg gatgcatgtc aagggcgagg acgacctggc ccgcctggcc acgtcgttca
      901 acgagatggc ggccagcctg cagcagcaga tcgcccagct ggagaacctg tcccgggtgc
      961 agcaacgctt cgtctcggac gtctcccacg agctgcggac gcccctgacc accattcgca
     1021 tggctggaga tctcatccac gatgcgcggg ccgacttcga gcccgccgtg gcccggtccg
     1081 cggagctgct gcacacccag atcgaccggt tcgaggccct gctggccgac ctgctcgaga
     1141 tctcccggtt cgacgccggg gcggccgcgc tcgacatcga gtccaacgac cttcggggca
     1201 tcgtggctca ggtcatcgag gtctgccagc cactggccga ccggcggggg agcgtcgtct
     1261 cgctgcgtgg tgcgcgccgt ccctgcatcg ccgatgtcga cctgcgccgg gtcgagcgcg
     1321 tcctgcgtaa cctgcttgtc aacgccatcg agcacggcga tggcggaccg atcgaggtcc
     1381 ggctggggca gagcgagacg gcggtggcgg tctccgtccg cgacgtcggc gttgggctca
     1441 agcccggcga gcgcgagctc gtcttcaccc ggttctggcg ggccgacccc gcccgggctc
     1501 gcacgaccgg cgggaccggc ctggggctcg cgatctccct ggaggacgcc cggctgcacg
     1561 ggggcagcct caaggcgtgg ggtgagctcg gtgaggggtc gtgcttccgg ctcaccctgc
     1621 ccaagaccgc gagccacccg gccggacggt cgccgctgcc cctgggtccc gtaccccgca
     1681 agagcgcacc gccggcgacg gtcgtggccg gcgccgaggc cgcctccgcg ggtgatgccg
     1741 gagccgggtc gtgaggcccc ggccccggct cggcaggcgt cgcggccggg ctctcctcgt
     1801 cgcggccgtc ctcggtggtc tgtgtgcctg cggaggcctg ccgacgagcg gtcccgtcgt
     1861 ctccgggcgt caggtcggcg ccggggtcct gcagccgatc cggatcgagc cgatcgggcc
     1921 gatcccgggg gctgacgcag gctcggtcgc gaccgggttc gttcaagccg gcgcggggtt
     1981 tcaggacaac accgacggcc ccgacgtcgc ccgcgacttc ctcactcccc gcgccgctgc
     2041 cgggtggaag cccacgtcgt cggtcaccgt gatcgaggcc ccggtctcgg tcgggtccgc
     2101 cgacgggagc cacgtccagg tgcgggccaa ggcggtggca cggatcgact cggccggcca
     2161 ctacaccgag gtcaggccgg gcacgttggt cacggccgtc ttcgccatcg agcgcctcgc
     2221 aggggagtgg cgtctcgacc tgcccgaggg cgggttcggg ctgtggctca cctcgcagga
     2281 cctccaacga ctcttcgaac ccgtccggat ctactatgtg accctctcgg ggcgcgaact
     2341 cgtcccggaa acccgatggt ttccctcagg cgcccggctc gccaccaccc tggcgcgagc
     2401 gcaagccgag ccggtcccgg catacctgcg cggtgctctt acgaccgggt tccccgaggc
     2461 gacctcgctg caggtcgacg ccgtgcccgt cgtcggcggc cgggcgaacg tcacgctgac
     2521 caccgccgca ctgcaggccg atcctgagca gcgccggatg atgtacgccc agatggtcac
     2581 ggcactgagc caggtgctca cggtccagga ggtcggcctg ctcgccgggg acaccaagct
     2641 cgagctgagc ggcctcgggg actccgcccg gtcgttcgcc gagctcggct accgcatcgt
     2701 cggcccggcg agcccgagga cgggactgct gcgccagggg tcgaccttgt cctcggtcaa
     2761 cctgcaacag ctggccggcc tggagcagga cacccgcccc agggccgtca cggcgcccgc
     2821 cgcgacctgg cccgcgatcg cccccggctg gacctcgctc gcgctgtcgg tcgacggcac
     2881 cgagctcgcg ggagtgggag gagacctcaa ggagctgcgc cgctggcgtg ggactgccga
     2941 gatccctatg gggcccttcg cctcgtcgct gacacgtccg tcctacgacg cccatgggtt
     3001 cctctggacg agcggggtca gcggggggac gaccagggcg tacgtcatcg acaccgcggc
     3061 ccggggtggc gcggcgcccg cggctgtcaa ggccgcctgg ctcgccccgc gccgggtggc
     3121 cgcgctgcgg gtctcccccg acggcacccg ggccgccgtc gtgtcccggg cgcccggcgc
     3181 ctcgaccgac tccctcgaca ttgccggagt catccggacc ctggccgggc agcccgtcgg
     3241 cctctccgcg cccgggccgg aaggccagcc gctgagcgag gtccgggacg tcacctggct
     3301 cgacatcgac acgctaggag tcctcggccg ggtgagcacc atcgagccct ggcgacccta
     3361 tgtcgtcgac ctcggccagg gcatcggcct gcgatcgcct ggttccgcgc tgctctcgcc
     3421 ggtcccggac ggccaggcga tcacgagtgc gggcggtccc cgtggggtgc tcgtccggac
     3481 cgagcgcggc cgggtcaagg tccgggccgg gagctcgtgg gtccagctct ccgctgggtc
     3541 cgaggtggtc gtccccggcg cctgaccggc gtccccaccc ccctcgcacg cagtcccgct
     3601 gtccacagcc gcgccggccg cgctcgcgtc cggagccacg cgagggcacc ctggcacggt
     3661 gcccccgccg tctgcgccgc gtggcaccag gcatccctgc tccgagggcg tgcgggcggg
     3721 gctcgcgagc ctgctcgacc tcgcccttcc cgccgagtgc gcgggatgct cgaggcccgg
     3781 gccggcgtgg tgccggcagt gtcgcgtggc cttcggctcg gagcacctgc gtgacggtcc
     3841 gtgcgccgtc gtgccctcac ccgagccccc agggctcccg ccggtcctct cgacgtaccg
     3901 gtatggcggg gcggtcacca tcgcgctgcg ggcctacaag gacgccggac gacgcgacct
     3961 cgggccggtg ctggcgccgg ccctcgcacc ggctctcacg ctcgccctgc gggtcgctgc
     4021 gcgcgacccg tccgcccgcg gat
//
LOCUS       DATAIX010000733         1941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_143696, whole genome shotgun sequence.
ACCESSION   DATAIX010000733 DATAIX010000000
VERSION     DATAIX010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143696"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1214)
                     /locus_tag="VHM65_10855"
     CDS             complement(87..1214)
                     /locus_tag="VHM65_10855"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX2706250.1"
                     /translation="MSAAGAGPSGVSEPSYRRGIFDEKLLAACISCGFCLPACPTYAE
                     TKLENSSPRGRITLMRALEEGRLDEDDPTLQHQAGLCLGCRACETVCPAGVRYGELLE
                     QWRDHQWRGRHTPPVAAALRAAVRVTPALAAGGLARGAARTTGAADRARPHLMLGCME
                     RSLFPKVSRAAARLVPEADVPSGQGCCGALHAHNGGSVEAAQMAARLGERMPGVIVTT
                     SGGCAAYLAHQLGHDRVKEVSEFLVERWARDPASMPVLRRLTVDGRPARVGLKDSCQL
                     RNGLGVTAQPRALIGRVAEYVELASAGVCCGGAGTYSILQPKMSQQVLDPTLQQARDA
                     ELDYLVALNIVCQRQLQTGVRKARLKVKVVHLVELLEMALE"
     gene            complement(1211..>1941)
                     /locus_tag="VHM65_10860"
     CDS             complement(1211..>1941)
                     /locus_tag="VHM65_10860"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2706251.1"
                     /translation="AVARTGVAYFDSLADAGRAVAGVIHDGIVPATLEFLDNRCINAV
                     EDFAQLGLDRDAGALLLFGDDGTAEAVERSLSIMAGTCERTGARGVTMARGIAESDAL
                     LAARRCALPALARLAPITLLEDATVPRPRIAEMVDRIDSICAAHHVVVATFGHAGDGN
                     LHPTIVFDPADDGAARRAEAAFAEIFGVAMDLGGTITGEHGVGAAKRPYLERRLGADQ
                     MALLRRLKSAFDPAGILNPGKLGS"
BASE COUNT          270 a          715 c          703 g          253 t
ORIGIN      
        1 ccgtgcccgc cagcagcggg caccgcatac gctcggaaaa cgcacacagc atccgtcggg
       61 ctgagttggc cgtcgaggac tccctgctac tccagcgcca tctccagcag ctcgacgagg
      121 tgcacgacct tgaccttgag ccgagccttc ctgacgccgg tctgcagctg gcgctggcag
      181 acgatgttga gcgcgacgag gtagtccagc tccgcgtcgc gggcctgctg cagggtcggg
      241 tcgagcacct gctgggacat cttcggctgc aggatcgagt aggtcccggc gcctccgcag
      301 cagacacctg cggacgccag ctcgacgtac tccgcgacgc gtccgatgag cgcacgcggc
      361 tgcgcggtga cgcccagccc gttgcgcagc tggcaggagt ccttgaggcc gacccgcgcc
      421 ggccggccgt ccaccgtgag ccggcgcagc accggcatac tcgcggggtc tcgggcccac
      481 cgctcgacga ggaactcgga gacctccttg acccggtcgt gcccgagctg gtgggcgagg
      541 taggccgcgc atccgcccga ggtcgtcacg atgacgccgg gcatgcgctc gccgagccgc
      601 gccgccatct gcgcggcctc gaccgaaccc ccgttatgcg catggagcgc cccgcagcac
      661 ccctggccgg agggaacgtc ggcctcgggg acgagccggg ccgccgcgcg gctcaccttg
      721 gggaacagcg agcgttccat gcagccgagc atgaggtgcg gacgggcccg gtcggccgcg
      781 ccggtcgtgc gggccgcccc gcgcgcaagg cctcctgcgg ccagggcggg ggtgacccgc
      841 accgcagcgc gcagcgcagc cgccaccggt ggcgtatgcc gcccgcgcca ctggtggtcg
      901 cgccactgtt cgagcagctc gccgtacctc acgcccgccg ggcagaccgt ctcacacgcc
      961 cggcagccga ggcacagccc ggcctggtgc tgcagcgtcg ggtcgtcctc gtcgagacgg
     1021 ccctcctcga gcgcgcgcat gagcgtgatg cgcccgcgcg gcgaggagtt ctccagcttg
     1081 gtctcggcat aggtcgggca ggccggcagg cagaagccgc acgagatgca cgccgcgagc
     1141 agcttctcgt cgaagatccc tctgcggtat gacggctcgc tcacgcctga agggccggcc
     1201 ccggcggcgc tcatgagccc agcttgccgg ggttgaggat gcccgccggg tcgaaggcgg
     1261 acttgagccg gcgcagcagc gccatctggt cggcgccgag acgccgttcc aggtagggcc
     1321 gcttggccgc cccgacgccg tgctcgcccg tgatcgtgcc gccaaggtcc atggcgacac
     1381 cgaagatctc ggcgaacgcc gcctcggccc ggcgcgcggc cccgtcgtcg gcggggtcga
     1441 agacgatcgt cgggtgcagg ttgccgtcac cggcatgccc gaaggtcgcg acgacgacgt
     1501 ggtgggccgc gcagatcgag tcgatccggt cgaccatctc ggcgatccgg ggcctgggca
     1561 ccgtcgcgtc ctcgagcagg gtgatcgggg ccagccgggc cagcgcgggc agggcgcagc
     1621 gccgggccgc gagcagcgca tcggactcgg cgatgcctcg ggccatggtc accccccggg
     1681 cgccggtgcg ctcgcacgtc ccggccatga tcgacagcga ccgctcgacg gcctcggccg
     1741 tcccgtcgtc accgaagagc agcagcgcac cggcgtcgcg gtccagcccg agctgggcga
     1801 agtcctcgac cgcgttgatg caccggttgt cgaggaactc cagtgtcgcc gggacgatcc
     1861 cgtcgtggat gactccggcg accgcgcgcc cggcgtcggc aagggagtcg aagtaggcca
     1921 ctccggtgcg tgccaccgcg g
//
LOCUS       DATAIX010000734         1659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_264572, whole genome shotgun sequence.
ACCESSION   DATAIX010000734 DATAIX010000000
VERSION     DATAIX010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264572"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VHM65_10865"
     CDS             complement(<1..238)
                     /locus_tag="VHM65_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX2706252.1"
                     /translation="MALATGGAATRAETDRIVWVDCEMTGLSLERDALVEVAALVTDF
                     ELNQLGEGVDLVVAPPPRALAQMDDFVRTMHTTSG"
     gene            271..1452
                     /locus_tag="VHM65_10870"
     CDS             271..1452
                     /locus_tag="VHM65_10870"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEX2706253.1"
                     /translation="MSRPRMLRDPAAQATFAAYPAVPNPTRRPLLRRLLLWGLTIIGF
                     LLSAAGLAYAFELELGVRATLVGAATAALPLAAVIPVFLWLDRYEAEPRRLKLFAFGW
                     GALVAPAVAIVLNTGSTQLLRASGVDDAQTLGAVFFAPVTEETTKGLAVLLVFWFARR
                     EVDGVIDGLVYAGLAAAGFALSENILYFGRAVAEGSEGALPAVFVLRGIMGPFAHPIF
                     TACTGIGIGIAAVSRSWLVRVVAIAVGWVGAVLLHALWNLSAQSGVQGYLTVYALVQV
                     PLFGAYVGLIGWSRRREGRLIGAHLAPYADAGWLTRPEVAMLASARGRHVARAWARRT
                     GGRRGRSSMQAFQDSASELALLRARVVQGSAGPHSAFTERTLLDAMAAHRRSFGGTPV
                     A"
     gene            1489..1561
                     /locus_tag="VHM65_10875"
     tRNA            1489..1561
                     /locus_tag="VHM65_10875"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1522..1524,aa:Arg,seq:tct)
BASE COUNT          196 a          587 c          597 g          279 t
ORIGIN      
        1 gcccagaggt ggtgtgcatc gtccggacga agtcgtccat ctgggccagg gcccgcggtg
       61 ggggcgcgac aacgaggtcg accccctcac cgagctggtt gagctcgaag tccgtgacga
      121 gcgctgccac ctcgacgagc gcgtcacgct cgagggacag gcccgtcatc tcgcagtcga
      181 cccagacgat gcggtcggtc tctgctcggg ttgctgctcc gcctgttgcc aacgccacgg
      241 accaacctta ggggcatagg gtgcgctgat atgagtcgcc ccaggatgtt gcgggatccg
      301 gctgcccagg cgacgtttgc ggcatacccg gccgttccca accccacccg ccgaccgctg
      361 ctgcgccggc tcctgctgtg gggtctgacg atcatcggct tcctgctcag cgcggcgggc
      421 ctcgcctacg cgttcgagct cgagctgggc gtccgggcga cgctggtcgg ggcggccacc
      481 gccgcgctcc cgctcgcggc cgtcatcccg gtcttcctct ggctcgaccg gtacgaagcg
      541 gagccgcggc gactcaagct cttcgcgttc gggtggggcg ccctggtcgc gccggccgtc
      601 gccatcgtgc tcaacacggg ctcgacgcag ctcctgcgcg cgagcggggt cgacgacgcg
      661 cagactctcg gggcggtctt ctttgccccg gtcaccgagg agaccaccaa gggcctcgcc
      721 gtcctgctcg tcttctggtt cgcccgccgc gaggtggacg gggtcatcga cgggctggtg
      781 tatgccgggc tcgcggcggc cggcttcgcg ctctccgaga acatccttta cttcggccgc
      841 gcggtggccg aggggtccga gggtgcgctg ccagcggtgt tcgtcctccg gggcatcatg
      901 gggccgttcg cccatccgat cttcaccgcc tgcaccggga ttggcatagg catcgccgcc
      961 gtgtcgcgct cgtggctcgt gcgcgtcgtg gcgatcgctg tcggctgggt cggcgcggtg
     1021 ctgcttcacg cgctctggaa cctgtccgcg cagtctggtg tccagggcta cctcacggtc
     1081 tatgcgctcg tgcaggtccc gctcttcggg gcgtacgtcg ggctcatcgg gtggagccgg
     1141 cgccgcgaag gccgcctcat cggcgcgcac ctggcaccgt atgccgacgc cggctggctc
     1201 acgcgcccgg aggtcgccat gctcgcctcg gcgcgcgggc gccacgtggc gcgggcctgg
     1261 gccaggcgaa ccggcggccg gcgcgggcgc tcctcgatgc aggcattcca ggacagcgcg
     1321 agcgagctgg ccctgctgcg ggctcgggtg gtccaaggat ccgcggggcc gcacagcgcc
     1381 ttcaccgagc ggacgctgct cgacgcgatg gcggcgcacc ggcggtcctt cggcggcacc
     1441 ccggtcgcgt gagcgggcgc ccgtagactg gtccgcggcg tgcgagcggc ccccgtagca
     1501 caacggatag tgcaacggcc ttctaatccg taggttgcag gttcgagtcc tgccgggggc
     1561 actctgcaag aagcgatcgg catccccgcg ccggatagtg gccctgcgcg ccgtctatat
     1621 gcatcgcgcg gagtgacttt gcggcgcgca gggccacta
//
LOCUS       DATAIX010000735         2693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_264734, whole genome shotgun sequence.
ACCESSION   DATAIX010000735 DATAIX010000000
VERSION     DATAIX010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264734"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VHM65_10880"
     CDS             complement(<1..509)
                     /locus_tag="VHM65_10880"
                     /inference="COORDINATES: protein motif:HMM:NF021220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX2706254.1"
                     /translation="MRGLVSGLDTPVPMLQQMPGVFQDSDFAARFVSAFDAALAPVFC
                     TLDNLAAYVDPGTAPEDLLAFVGFWVGARREDQTSVPMQRLAVADAVRTHRLRGTAEG
                     LALVVRHMTGGEVQVTESGATAWSATAGGQLPGTGPPRVSVRVVVDDASAVDVELVRA
                     AVAEVVPPYV"
     gene            complement(513..2453)
                     /locus_tag="VHM65_10885"
     CDS             complement(513..2453)
                     /locus_tag="VHM65_10885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HEX2706255.1"
                     /translation="MPIPLPNLDDRRFQDLVDDAKRMVQQSCPEWTDHNVSDPGVTLI
                     ETFAYLVDTLLYRLNRIPDKLYLAFLDLIGVSPHPPTAASVEATFWLSAPQDHDVTVH
                     RGTHVSTVRTEQDEAVVFETTRDLVIPVRSMTAVATQARDGDVVARAIEVREGSGFAA
                     FTTSPRPGDAVLVALDEAAPLCAVALRWDCEVKGVGVNPEHAPLRWEAWTGTGWVACD
                     TERDGTGGLNRPGYIIVHLPAGHVASVIGGVRGGWVRTVVTEPEPDYPAYVSSPLVRS
                     VEAQTVGGTVRAVHAEEVFDEVVGLSEGIPGQSFPLARTPVVDDGRPMVLEVASGPGW
                     LEWQEVSSFADQSAADLVFRVDRAAGRVELGPAVREPDGTVTRFGAVPPKGAPLRVPA
                     YRTGGGPRGNVAARAISVLRTSVPLIERVENRRTASGGTAGETIEQVRVRGPLDLRTR
                     DRAVTAADIEVLARRAAPSIARVRAIAEGDYGARLLVVPVAPVEADGRVRFEDLVPPE
                     ATLAAVTEFLDTRRVIGTRLVIEPPFYQGVTVVARVLARRRTDTDSLREAALAALYQH
                     FSPLTGGPDGEGWPFGRPVHVGEVYAVLQRLPGTELVEEVRLFAADPTTAVRGDPTDR
                     IALDANALVFSFGHQVKVIKAG"
     gene            complement(2453..>2693)
                     /locus_tag="VHM65_10890"
     CDS             complement(2453..>2693)
                     /locus_tag="VHM65_10890"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEX2706256.1"
                     /translation="DYVFAPADASTAGDIAYTVRVALDRWEPRIELEGVDVRFDEVEQ
                     GTLYIDIHYAVRGTNDPRNLVFPFYVIPPTERSGS"
BASE COUNT          416 a          991 c          917 g          369 t
ORIGIN      
        1 acatacggtg ggactacctc ggcgacggcc gccctcacca gttccacgtc caccgcggag
       61 gcgtcgtcca caacgacacg cacgctcacc cgcggcggac ccgttcccgg caactggcca
      121 ccagcggtcg ccgaccaggc cgtggcccct gactcggtca cctgcacttc acctccggtc
      181 atgtgccgga ccaccaatgc gagcccctcc gccgtgccac gcaggcggtg agttcgcacc
      241 gcgtcggcca cggccagccg ctgcatcggg acggaggtct ggtcctcccg gcgcgccccg
      301 acccagaacc ctacgaaggc aagcaggtcc tcgggcgcgg tcccggggtc gacgtatgcc
      361 gcgaggttgt cgagcgtgca gaacaccggg gcaagtgccg cgtcgaaggc ggagacgaac
      421 cgcgccgcga agtcgctgtc ctggaacact cccggcatct gctggagcat cgggacgggc
      481 gtgtccagcc cggagaccaa cccacgcatc gctcagcccg cctttatgac cttgacctgg
      541 tgcccgaacg agaagacgag tgcgttggcg tccaaggcga tccggtcggt cgggtctccc
      601 cgcacggccg tcgtcgggtc ggcggcgaag aggcggactt cctcgacgag ttcggtgccc
      661 ggcagtcgct gcagcacggc atacacctct cccacgtgca ccggtcgccc gaacggccag
      721 ccctcgccgt ccggcccacc ggtgagcggg ctgaagtgct ggtagagcgc cgccagcgcg
      781 gcttcgcgca gggagtcggt gtcggtgcgc cgccgggcca ggacgcgggc caccacggtc
      841 accccctgat agaacggcgg ctcgatgacg agccgggtcc cgatgacccg acgggtgtcg
      901 aggaactcgg tcaccgcggc cagcgtcgcc tccggcggca cgaggtcctc gaaccgcacc
      961 cttccgtcgg cttcgaccgg cgccaccggc accacgagca gccgtgcacc gtagtcgccc
     1021 tcggcgatgg cgcgcaccct ggcaatcgac ggcgcagccc ggcgagccag gacctcgatg
     1081 tcggccgccg tcaccgcccg gtcccgagtg cgcaggtcga gcggtccacg aacccggacc
     1141 tgctcgatcg tctccccagc ggtcccgccg cttgccgtgc ggcggttctc gacccgctcg
     1201 atcaggggta cggacgtgcg cagcaccgag atcgcccgcg cggccacgtt cccgcgcggc
     1261 cctccgccgg tccggtaggc cggcacgcgc aggggggcgc ccttgggcgg taccgccccg
     1321 aaccgggtta cggtcccgtc cggctcacgc accgccggcc ccagctcgac ccgtccggcc
     1381 gcccggtcca ctcggaagac caagtcggcg gcactctggt cggcaaacga ggagacctcc
     1441 tgccactcca gccaccccgg cccggaagcg acctcgagca ccatcggccg gccgtcgtcg
     1501 acgaccggcg tacgggccag cgggaaggac tgaccaggga tcccctcgga gagcccgacc
     1561 acctcgtcga acacctcctc ggcgtgcacg gccctgacgg tcccgcccac ggtctgtgcc
     1621 tccaccgatc gaacaagggg cgaactcacg taggccgggt agtccggctc gggctcggtc
     1681 accacggtcc ggacccaacc gccgcgcacc ccgccgatga cggaggcaac gtgcccggca
     1741 ggcagatgga cgatgatgta gccaggccga ttcagcccac cggttccatc gcgctcggta
     1801 tcgcaggcga cccacccagt cccggtccag gcttcccagc gcagcggggc gtgctcgggg
     1861 ttgaccccga cgcccttgac ctcgcagtcc cagcgcagcg ccacggcgca cagtggcgcc
     1921 gcctcgtcga gcgccaccag cacggcgtcc cccggtcgag ggctggtggt gaaggcggcg
     1981 aagccgctgc cctcccgcac ctcgatcgcc cgcgccacga cgtccccgtc acgagcctgg
     2041 gtcgccaccg ccgtcatcga ccgcaccggt atgacgaggt cacgggtggt ctcgaagacg
     2101 accgcctcgt cctgctcggt ccgcaccgtg ctcacgtggg tgccccgatg cacggtgacg
     2161 tcgtggtcct gtggtgcgga gagccagaag gtggcctcga cggacgcggc cgtgggcggg
     2221 tgcgggctga cgccgatgag gtcgagaaag gccaggtaga gcttgtcggg aatccggttg
     2281 agccggtaga gcaacgtgtc gacgaggtag gcgaaggtct cgatgagggt gacacccggg
     2341 tcggacacat tgtggtcggt ccattccgga cagctctgct ggaccattcg cttggcgtcg
     2401 tcgacgaggt cctggaagcg gcggtcgtcg aggttgggca gcgggatcgg catcaggatc
     2461 cgctccgttc ggtcggtggg atgacgtaga acggaaacac caggttgcgc gggtcgttgg
     2521 tgccgcgcac ggcgtagtgg atgtcgatgt acaaggtccc ctgctcgacc tcgtcgaagc
     2581 ggacgtcgac gccctcaagc tcgatccgcg gctcccaccg gtcaagagcg acccgcaccg
     2641 tgtaggcgat gtcaccggcg gtgctcgcgt ccgccggagc gaagacgtag tcg
//
LOCUS       DATAIX010000736         3221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_264757, whole genome shotgun sequence.
ACCESSION   DATAIX010000736 DATAIX010000000
VERSION     DATAIX010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264757"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(434..1429)
                     /locus_tag="VHM65_10895"
     CDS             complement(434..1429)
                     /locus_tag="VHM65_10895"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEX2706257.1"
                     /translation="MDLDAYVAAHRHQWQRLEQLGAKRRLTGAEADEVLDLYQRVSTH
                     LSLIRSTTPDPTIVAHLSVLLARARSKSAGTRTGSWRSVGTFFTQTFPGALYRTRRWW
                     LVTLVLNLVVAFVMGWWFLVNPQVESALATPAEIDQLVNADFEGYYSEFAAGSFAFRV
                     WTNNAWVSALCISLGILGFPVITLLYNNILNVSLIGSIMWHHDRAGLFFGLILPHGLL
                     ELTAVFVAAGTGLRIFWGWVEPGPRTRMASVAHEVRSAMAVALGLVVVLLVTGVIEAF
                     VTPSGLPTWARIGIGLLAEAAFFTYVFTVGRRAARAGVTGDVGAEDLETLAPTSA"
     gene            1496..2332
                     /locus_tag="VHM65_10900"
     CDS             1496..2332
                     /locus_tag="VHM65_10900"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX2706258.1"
                     /translation="MPGERSHTSWASDDDLVTGEAVALELPAASIGPRVLSGAIDLIV
                     AFVLFYAVLIAWTATLSAMGEIDDDALVSAVALVALVVIVVGLPAAVETLTRGKTLGK
                     LIVGLRTVRDDAGPITFRHAFTRAMVGVVEIYAFAGGPAVICALVTGKGKRLGDIAAG
                     TYVIRERVRSTLPTPIPMPPHLADWATTADMTALPDSLAMSVRAFMVSTHQLAPGARA
                     QVGQDLAARVLCYVAPPPPPGQHPEYVLAAVVADRRRRDLQRLARDAAIRASVVPPDP
                     LG"
     gene            complement(2395..>3221)
                     /locus_tag="VHM65_10905"
     CDS             complement(2395..>3221)
                     /locus_tag="VHM65_10905"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HEX2706259.1"
                     /translation="PELHIAAGLTLSDVVEAVNAGLRQGERLAAEAGHRIQARALLTA
                     MRHAAKSREIAELVVRYRDDGVGGFDIAGAEAGYPPTRHLDAFEYLSRANAHFTIHAG
                     EAFGLPSIWEALKWCGADRLGHGVRIVDDITIDGLPFAEWVETGVTSLADVDLASVYL
                     GRLAAYVRDARIPLEMCPSSNLQTGAAHTIALHPITLLKNLRFRVTLNTDNRLMSGTS
                     MTREMALLADEARWTMSDLRWVTINAMKSAFLPFDHRLEVINEQIKPGYAAAGVLA"
BASE COUNT          521 a         1157 c         1115 g          428 t
ORIGIN      
        1 ccgtagtggt gcggtgaggg ggcaggacga cgcgcaccga ccagcccgtg cgccgcacca
       61 gaccggtcat gagcccgtcg cgcggaccgg ggccgagccg ggctccgatg taggccgccg
      121 ttgccacacc ggtgacgagg acgttgctca ccgtgcccac ccccgggtcg ttctcgcaag
      181 ggcagccaca gcacgagcac gagggcgccg acgatgaccg accacgtgcc gatggagagc
      241 cccagccgct ggaccagacc ctggtgcagg acgtcccacg ggatgacgcc gagtccagca
      301 cggatgacca gcgcctcacc gccccgcaaa ggtgacgagc ccggcgtaca gctgcatgaa
      361 ccgtcgccac aggcggccgt caggagggtc gctcggcacg acgtcatagt cgctcacacg
      421 accgggcgcc cgctcatgcc gacgtcgggg cgagggtctc gaggtcttcg gccccgacat
      481 ctcccgtcac gccagcccgg gcagcacgcc gcccgacggt gaagacgtag gtgaagaagg
      541 cggcctcggc gagcaggccg atcccgatac gcgcccacgt cggcaggccc gagggggtga
      601 cgaacgcctc gatgacaccg gtcaccaaga gcacgacgac gagcccgagc gcgacggcca
      661 tcgccgagcg cacctcgtgc gcgaccgagg ccatccgggt ccggggcccg ggctcgaccc
      721 acccccagaa gatccgcaga cccgtgcccg cggcgacgaa caccgcggtg agctcgagca
      781 ggccgtgcgg caggatcagg ccgaagaaga gccccgcgcg gtcgtggtgc cacatgatcg
      841 acccgatgag cgacacgttg aggatgttgt tgtagagcag cgtgatcacc ggaaaaccga
      901 ggatccccaa cgagatacac aacgccgaca cccaggcgtt gttcgtccac acccgaaacg
      961 cgaagctccc cgcagcaaac tcgctgtagt agccctcgaa gtcggcgttg acgagctggt
     1021 cgatctcggc cggggtcgcc agggccgact cgacctgcgg gttgacgagg aaccaccacc
     1081 ccatgacgaa cgccaccacg aggttgagga ccagcgtgac gagccaccag cgccgggtgc
     1141 ggtagagcgc cccagggaac gtctgcgtga agaaggtccc gacgctgcgc cacgagcccg
     1201 tgcgagtacc cgccgacttg gacctggccc gggcgagcag cacggacagg tgcgcgacaa
     1261 tcgtcgggtc gggagtcgtc gaccggatca gggacaggtg ggtggacacc cgctggtaga
     1321 ggtcgaggac ctcgtcggcc tcggccccgg tcagccgccg cttggcaccg agctgctcga
     1381 ggcgctgcca ctggtgccgg tgcgctgcga cgtaggcgtc cagatccacc ccgacactgt
     1441 atgacgaccg cgctggatga cgaccgcgag aacgcaggcc cagtaggctg gccgggtgcc
     1501 gggggaacgc agtcacacct cgtgggccag cgatgacgac ctcgtcaccg gtgaggccgt
     1561 cgcgctcgag ctgcccgcag cctcgatcgg cccccgcgtc ctgtccgggg ccatcgacct
     1621 catcgtggcg ttcgtcctct tctatgccgt gctcatcgcc tggacggcca cgctctccgc
     1681 gatgggcgag atcgacgacg acgcgctcgt gtccgccgtc gccctcgtcg cactggtcgt
     1741 catcgtcgtc gggctgcccg ccgcggtcga gacgctgacc cggggcaaga cactggggaa
     1801 gctcatcgtc gggctgcgga cggtgcgcga cgacgccggc cccatcacct tccggcacgc
     1861 cttcacccga gccatggtcg gggtcgtcga gatctacgcg ttcgccggag gccccgcggt
     1921 catctgcgcg ctcgtcaccg gaaagggcaa gcggctcggg gacatcgccg cggggaccta
     1981 cgtcatccgc gagcgggtgc gctcgacctt gccgacaccg atcccgatgc caccgcacct
     2041 ggccgactgg gcgaccaccg cagacatgac ggcgctgccg gactcgctgg ccatgtcggt
     2101 gcgcgcgttc atggtgagca cccaccagct cgctcccgga gcgcgggccc aggtcgggca
     2161 ggatctcgcg gcacgggtgc tgtgctacgt ggcgcccccg ccgccgcccg gccagcaccc
     2221 ggagtacgtc ctggcggccg tggtcgcaga ccgtcgtcgc cgcgacctgc agcggctggc
     2281 ccgggacgcg gcgatccggg ccagcgtcgt gccgcccgac ccgctgggct gaccacgagg
     2341 cggctacccg aggcgccccg ccacgtgagc acccccgcaa cgcgagaacc cccgctacgc
     2401 gagcaccccc gcggcggcat acccgggctt gatctgctcg ttgatgacct ccaggcgatg
     2461 gtcgaagggc aggaaggccg acttcatcgc gttgatcgtg acccagcgca agtcgctcat
     2521 tgtccacctc gcctcgtccg cgagcagggc catctcgcgg gtcatcgacg tccccgacat
     2581 gagccggttg tccgtgttga gggtgaccct gaagcgcagg ttcttcagca aggtgatcgg
     2641 gtggagcgcg atcgtgtggg cggccccggt ctgcaggttg ctcgacgggc acatctccag
     2701 ggggatccgg gcgtcccgca cgtaggctgc gagccggccg aggtagacgc tggccaggtc
     2761 gacgtcggcc agggaggtga cgcccgtctc gacccactcc gcgaacggca gcccgtcgat
     2821 cgtgatgtcg tcgacgatcc ggacgccatg gccgagccgg tcggcgccgc accacttcag
     2881 ggcctcccag atgctcggca gcccgaacgc ctcaccggcg tggatcgtga agtgggcgtt
     2941 ggcgcggctc aggtactcga acgcgtcgag atgccgagtg ggcggatagc ccgcctcagc
     3001 cccggcgatg tcgaagccgc cgacgccgtc gtcgcggtag cggacgacga gctcggcgat
     3061 ctccctgctc ttggccgcgt ggcgcatcgc cgtgagcagc gcccgcgcct ggatccggtg
     3121 acccgcctcg gcggcgagcc gctcgccctg gcgcagaccc gcgttgacgg cctccacgac
     3181 gtcgctcagg gttagcccag cagcaatgtg cagctctggg g
//
LOCUS       DATAIX010000737         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_144187, whole genome shotgun sequence.
ACCESSION   DATAIX010000737 DATAIX010000000
VERSION     DATAIX010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144187"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="VHM65_10910"
     CDS             complement(<1..1133)
                     /locus_tag="VHM65_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX2706260.1"
                     /translation="MRRAFVILNVGLAVTLVGVGAAAVLAVGDPTSAATTTTTPTVKA
                     SRGTLTATVSASGNLSAKTSIAANFTGSGGTVTEILVKVGDTVTAGQALAKVDDASAK
                     HSLASAKASLQQAQAGLSTTTQKPTSQQKARSSSQVRQAQVSVQNAQLSLSQARATYA
                     RDRTTQNLLVDQAQEAYDAATDATRASMKNALSQATATRASTLLRGSQSIQTAQGQVT
                     SAQAALSAQKASVAVDAQPARSGSVAASKAQVAAAEVQVAQAQLTLDQTVLEAPVSGT
                     VVSISGEVGASSSAAASSSSSSSSSSTSSASTAFLTIATMDQLQVTTMVAEADAAKVQ
                     TGQMATVSFSALGTSASGSVTAIDLTQTISNNVVEYGVTVLLDS"
     gene            complement(1373..>2612)
                     /locus_tag="VHM65_10915"
     CDS             complement(1373..>2612)
                     /locus_tag="VHM65_10915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2706261.1"
                     /translation="SAPAPRTTFCPLPADLKVPVPQLGAAGGQVQFIDDQGVVTLTPD
                     GGLQLPVSGAARALAAAQSGPDLKEDTVVGGIPVRVVTHAIGHGRAVQVGLRLDGIES
                     VLASLRWILLAVSLVGVAIAVGLGRWIAGSAMRPVSRLMVATEHVAGTHDLRERIAEP
                     GNDELGRLAHSFNRMLEALDSSEHLQRQLVADASHELRTPLSSLRTNIEVLALGRGLS
                     DDDRERLLASLTGQVERLSRLVADLIDLARGDEPLEQVFVETRLDQLVAASVEDARAH
                     YPGLQIELSAPPCLVTGDPGRIRRAIDNLLDNAGKWSPVGGQVDVTVGDGEVSVQDHG
                     PGVERADREHVFDRFWRAPAARSTPGSGLGLAIVQQVARAHGGEVRLESPADGGSRFV
                     LRLPVAGQALDVEGDVEPEV"
BASE COUNT          351 a          886 c          962 g          413 t
ORIGIN      
        1 gagtcgagga ggaccgtgac cccgtactcg acgacgttgt tgctgatggt ctgggtgagg
       61 tcgatggccg tcacgctgcc gctcgcgctc gtcccgagcg cggagaacga gacggtcgcc
      121 atctggcccg tctgaacctt cgctgcgtcc gcctcggcga ccatcgtggt cacctggagt
      181 tggtccatcg tggcgatggt caggaaggcc gttgacgcgg acgaggtgga cgagctcgac
      241 gagctggacg agctggacgc cgccgcactc gaggacgcgc ccacctcgcc ggagatcgag
      301 acgacggttc cgctgaccgg ggcctcgagc accgtctggt cgagggtcag ctgcgcctgg
      361 gcgacctgga cctcggcggc ggcgacctgc gccttggacg cggcgacgga gcccgagcgt
      421 gcaggctgcg cgtcaacggc aacggatgcc ttctgcgcgc tgagcgcggc ctgcgcggag
      481 gtgacctggc cctgggccgt ctggatcgac tgcgagccgc ggagcagcgt gctcgcccgc
      541 gtggccgttg cctggctcag ggcgttcttc atgcttgctc gggtcgcatc ggtcgcagcg
      601 tcgtaggcct cctgcgcctg gtcgacgagc aggttctgag tcgtcctgtc gcgggcgtac
      661 gtggcgcgcg cctggctcag cgagagctgc gcgttctgca cgctgacctg ggcctgccgg
      721 acctggctcg agctgcgggc cttctgctgg ctggtcggct tctgggtggt ggtcgagagg
      781 cccgcctggg cctgctgcag gctcgccttg gcggatgcca gtgagtgctt cgcgctggcg
      841 tcgtccacct tcgcgagagc ctggcccgca gtgacggtgt ccccgacctt gacgaggatc
      901 tcggtcacgg tcccgcccga gccggtgaag ttggcggcga tcgaggtctt cgccgacagg
      961 ttgccgctcg ccgagaccgt ggccgtcagc gtgccgcggc tcgctttgac ggtcggggtg
     1021 gtggtggtcg tcgcggcgga ggtcgggtcg ccgacagcga ggaccgcggc cgcgccgaca
     1081 ccgacgaggg tgacggcgag gccgacgttg aggatcacga aggctcgtct catgacgcct
     1141 tcgctcccgt cgggacgggg gcgttcatcg gtttgctcct tgaaggtgcg aggtggcgct
     1201 cgccgcgcat tgcgggctgg ctgcctattc aaggggctgg acctaagaac ggcttaaggg
     1261 gcggctatga atgccctcag gaagtgccgg accccacctt ccggtcgcgg ccgctcgtcg
     1321 aggagccggc tcgtgcttca ggcgccgccg gggcgggggg cgtcgcgtcg ggctagactt
     1381 cgggctcgac gtcaccctcg acgtcgagcg cctggcccgc gaccggcagg cgaagcacga
     1441 accgcgaccc gccatcggca ggcgattcga ggcggacctc gccgccgtgg gcccgcgcca
     1501 cctgttggac gatggccagc cccagacccg atcccggtgt ggagcgggcc gcgggcgcgc
     1561 gccagaatcg gtcgaagacg tgctcgcggt cagcacgttc gacgcccggt ccgtggtcct
     1621 ggacggacac ctcaccatcc ccgactgtga cgtccacctg tccgcccacg gggctccact
     1681 tgccggcgtt gtcgagcaga ttgtcgatcg cccgcctgat ccgccccggg tcgccggtga
     1741 cgaggcaggg tggcgccgag agctcgatct gcagccctgg gtagtgagcg cgcgcgtcct
     1801 ccacggacgc ggcgacgagc tggtccagcc gcgtctccac gaagacctgc tcgaggggct
     1861 cgtcgccgcg ggcgaggtcg atgaggtccg cgaccagccg gctgagccgc tcgacctggc
     1921 cggtcagcga ggcaagcagt cgttcgcgat cgtcgtcgga aaggccacgt cccagggcca
     1981 ggacctcgat attcgtccgc aggctgctga gcggcgtccg aagctcgtgc gaggcgtcgg
     2041 cgaccagctg gcgctggagg tgctcggacg aatcgagcgc ctcgagcatc cggttgaagc
     2101 tgtgggcgag tcgacccagc tcatcgttgc cgggctcggc gatacgctcc cggaggtcgt
     2161 gcgtcccggc gacgtgctca gtcgcgacca tcaggcgcga taccgggcgc atcgcggatc
     2221 ccgcgatcca gcgaccgagt cccacggcga tcgccacgcc gaccagcgag acggccagca
     2281 ggatccagcg gaggctggcg agtacgctct cgatcccgtc gagccgaaga ccgacctgca
     2341 ccgccctgcc atggccgatc gcatgcgtca ccacgcggac cgggatgccg ccgaccaccg
     2401 tgtcctcctt gaggtccgga ccggactggg ctgctgccag tgctcgcgcc gccccgctga
     2461 ctggcagctg cagaccccca tcgggggtta gggtcactac gccctggtcg tcgatgaact
     2521 gcacctggcc gcccgcggcg ccgagttgcg gaaccggcac cttcaggtct gccggcagag
     2581 ggcagaacgt ggtccggggc gcaggcgcgg ag
//
LOCUS       DATAIX010000738         4312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_144413, whole genome shotgun sequence.
ACCESSION   DATAIX010000738 DATAIX010000000
VERSION     DATAIX010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144413"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VHM65_10920"
     CDS             complement(<1..578)
                     /locus_tag="VHM65_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706262.1"
                     /translation="MGRDENDNPVRRHVRGRTRLTVTDKVAALENQRAKGAGRSATNS
                     RQTVGGWLDEWLLLVRRSRKARTFTTYDSLVRTHCAGIRTTPLNRLTIRQLDDLLNHV
                     ATTVSPTSAANLHRTLRAAFSVAVRRGLIPANPCRYATVPRVPHVEVEPLTLSEVRCL
                     LAAAGQDRNPARWSVALALGLRQGEALALQWC"
     gene            complement(656..964)
                     /locus_tag="VHM65_10925"
     CDS             complement(656..964)
                     /locus_tag="VHM65_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2706263.1"
                     /translation="MAEMTAVTIRSIATRIEQRLRAIAEHEAAIRCDLAMLSALTPES
                     RGDGLVDIAAAAGWLGVSRATMFRLLRDDPYLHHLKVGNRTLFRPEDLRAYAARRVAS
                     "
     gene            complement(1118..1189)
                     /locus_tag="VHM65_10930"
     tRNA            complement(1118..1189)
                     /locus_tag="VHM65_10930"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1155..1157),aa:Lys,seq:ttt)
     gene            complement(1229..4300)
                     /gene="eccCb"
                     /locus_tag="VHM65_10935"
     CDS             complement(1229..4300)
                     /gene="eccCb"
                     /locus_tag="VHM65_10935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccCb"
                     /protein_id="HEX2706264.1"
                     /translation="MTVLTIGGTPPPASDVAPVLVLGTGRDSAGGAAGEVSLRVIPAG
                     GEPVDGWADQLSIAGADALARRLAAWGRDERPRGRTGDGVSPELVDLLGLGDLRRLRL
                     SRAWAPRGARDRLRVPIGVDDAGAPVWLDLKEAAEHGMGPHGLLIGATGSGKSELLRT
                     VVVGLALTHSPEDLNLVLVDFKGGATFAGLAGMPHVSAVITNLREELTLVDRMYDALS
                     GEMLRRQQVLRAAGNVASLRDYNAARSAGRALDPLPTLVIVCDEFTELLTAQPEFTEL
                     FVAMGRLGRSLGIHLLLASQRLEEGRLRGLDSHLSYRIALRTFSASESRAVIGEPDAY
                     VLASVPGLGYLKPGPSTRVRFRATYVSGPAGAAERTLPNRAVVTPIVPFRVGEVRALP
                     GALARGPAGDTLVRRSRKANSDRPARACVGPTVVDLAVSAMSGQGPEAHRVWLTPLGA
                     PDTFDTLLPDLIADPERGLHSPSWRARGPLRVPLGAVDRPKDQRRDILVADLSGPGGH
                     VAVVGGPQTGKSTVLRSLVAGLALTHTPREVQVFVLDFGGGTVAGLRDLAHVAGVATR
                     SEPDVVRRICAEVATIADERERYFRSAGIDSIAAYRSRRAAGAADDGYGDVVLVVDGW
                     ATLRAELDGLEAELQALAERALTYGVHLLTSTTRWLDYRASMRDLLGTRVELKLGDAM
                     DSEVDRKLAAAVPGGRPGRAITPEGFHVLTALPRLDGAADPATLAAGVEHLVSAVNDA
                     WTGPPGPKLRLLPDTVSLSDLRSCADEPTSGRLLVGLTERSLRPVGLDLGEEPHLLVY
                     GDGRSGKSAFLRSYVRELERLSDPGRSQLFVVDVRRAHLGEYDDAWVAAYCTTAGAAA
                     STMTELASYLGTRLPGPGVTAAQLRARSWWTGREVYVLVDDYELVASAQPNPLAPLLA
                     LLPQARDVGLHLVLARRTGGAGRSFDPVSQALRDLGSPGLILSGDPAEGALLGGVRPS
                     LSAPGRARLVRRDGVQVVQLAWSPPGGVPEGTQPPRPADRAAGHLLVSPVD"
BASE COUNT          642 a         1489 c         1612 g          569 t
ORIGIN      
        1 tcgcaccact gcagagcaag ggcttcccct tgacgcagtc ccagggccag agcaacggac
       61 caacgggctg ggttgcggtc ttgaccggcg gcggcaagaa ggcatcggac ctccgacagg
      121 gtcaggggtt ccacctccac gtgcggcacg cgggggacag tggcgtaccg gcaagggttt
      181 gccggtatca agccgcgcct tactgccacg gagaaggccg ctcgaagcgt tcggtgaagg
      241 ttcgccgcac tggtggggct gaccgtggtc gcgacatggt taagaaggtc atcgagctgg
      301 cggatggtca gccggttcag cggtgttgtc cgtattcccg cgcaatgggt gcgaacgagc
      361 gagtcatacg tggtgaaggt gcgcgccttg cggctgcgtc gaacgagcag tagccactca
      421 tcgagccagc ctcctacggt ctggcgactg ttcgtggcag aacgtcccgc gcccttggcg
      481 cgctggtttt ccagggcggc gaccttgtca gtgacggtca atcgggtacg tccgcgcacg
      541 tgtcgacgaa ccgggttgtc gttctcgtct cggcccatcg aaacccaacc gtgccaggtt
      601 ccgtcggctc ctctatagat ggatgaacgg ccattggcgg atctgctgct gcggctcatg
      661 aagccacgcg cctcgcggcg taggctctca ggtcctctgg ccggaaaagg gtgcggttgc
      721 cgaccttcag atgatgcagg tacgggtcgt cccgcagaag ccgaaacatc gtcgcgcggg
      781 agactccgag ccagcctgcg gcggcagcga tgtcaacgag accgtcgccc cggctctctg
      841 gggtcagcgc ggacagcatc gccagatcgc atcggatggc ggcctcgtgt tcggcgatag
      901 cgcgcagccg ctgctcgatg cgcgtggcga tggagcggat cgtgactgcg gtcatctcgg
      961 ccataccgtc cgaaagcggt cggatggcac ccggtgtcca tcgcgatacg ggctgagacg
     1021 cgcaactgca ccgcgcaact gcaccgcaag ggggttttga cgtctcatcg gacctccaaa
     1081 atctgaccga tgaggctctg aactgcggct ttgtgggtgg gccccgtcgg actcgaaccg
     1141 acaacctacg gattaaaagt ccgcagctct gccattgagc tagaggccct ggcgccgccg
     1201 acgagggcac cagcggaccg cgacgagcct agtccacggg gctgacgagc aggtgcccgg
     1261 cggcgcggtc cgcgggccgg ggcggctgcg ttccctcggg aaccccaccc ggcgggctcc
     1321 aggcgagctg gacgacctgc accccgtccc gccggacgag ccgcgcgcgc ccgggcgcgg
     1381 acagggacgg ccggaccccg cccaggagtg ctccttcggc ggggtcgccg ctgaggatca
     1441 ggccgggaga gccgaggtcg cgcagggcct ggctcaccgg gtcgaacgag cgccccgcgc
     1501 cacccgtgcg cctcgcgagg acgaggtgaa ggccgacatc gcgcgcctgg gggagcagcg
     1561 ccagcagcgg cgcgagcggg ttgggctgcg ccgacgcgac gagctcgtag tcgtcgacga
     1621 ggacgtagac ctcacgtccg gtccaccagg accgtgcgcg cagctgcgcg gcggtgacgc
     1681 ccggtccagg caggcgagtg ccgaggtatg acgcgagctc ggtcatcgtg ctcgccgcgg
     1741 cgccggcggt ggtgcagtag gcagccaccc acgcgtcgtc atactcgccg aggtgggctc
     1801 gtcgcacgtc gacgacgaag agctgggacc ggccgggatc gctgagccgc tcgagctcgc
     1861 ggacgtacga gcgcaggaag gccgacttgc ccgaccgccc gtcgccgtag acgagcaggt
     1921 gcggctcctc gcccaggtcg agaccgaccg gacgcaggga ccgctcggtg aggccgacga
     1981 ggagccggcc cgaggtgggc tcgtcagcgc acgagcgcag gtcggagagc gacacggtgt
     2041 cggggagcag ccgcagcttg ggcccgggcg gccccgtcca ggcgtcgttg acggccgaga
     2101 cgaggtgttc gacgcccgcg gcgagcgtgg ccgggtcggc cgctccgtcg aggcgcggca
     2161 gggcggtgag cacgtggaag ccctccggcg tgatggcccg gcccgggcgc cctccgggga
     2221 ccgcggccgc gagcttgcgg tcgacctcgg agtccatcgc gtcgccgagc ttgagctcga
     2281 cccgggtccc gagcaggtca cgcatcgacg cgcggtagtc gagccagcgg gtggtgctcg
     2341 tcaggaggtg gacgccatac gtgagcgccc gctcggccag cgcctggagc tcggcctcga
     2401 gcccgtcgag ctcggcgcgc agggtggccc acccgtcgac gacgaggacg acgtcgccat
     2461 agccgtcgtc ggccgctccc gcggcccggc gggaccggta ggccgcgatc gagtcgatcc
     2521 cggccgaccg gaagtagcgc tcgcgctcgt ccgcgatcgt ggccacctcg gcgcagatgc
     2581 gccggacgac gtcaggctcc gagcgcgtcg cgactccggc gacgtgcgcg aggtcgcgca
     2641 gccccgcgac ggtgccgccg ccgaagtcga ggacgaacac ctggacctcg cgcggcgtgt
     2701 gggtcagcgc caggccggcg acgaggctgc gcagcaccgt cgacttgccg gtctgcgggc
     2761 ctccgacgac ggcgacatga cctccgggac cggacaggtc ggcgacgagg atgtcacggc
     2821 gctggtcctt ggggcggtcg acggcaccga gcgggacgcg cagcggcccg cgagcgcgcc
     2881 aggagggcga gtgcagcccc cgctcagggt cggcgatcag gtcgggcagg agcgtgtcga
     2941 aggtgtcggg ggccccgagg ggcgtcagcc agacccggtg ggcttccggg ccctgcccgc
     3001 tcatggcgga caccgcgagg tcgacgacgg tagggccgac gcaggctcga gccggcctgt
     3061 cgctgttggc ttttcgcgac cttctgacca acgtgtcacc agcggggccc cgcgcgagcg
     3121 cgccggggag cgcccggacc tcgcccacgc ggaacggcac gatcggcgtc acgacggcac
     3181 ggttcggcag agtgcgctcc gccgcgcctg cagggcctga gacgtatgtc gcgcggaacc
     3241 gcacccgcgt cgacgggccg ggcttgaggt agccgagtcc ggggacgctt gccagcacgt
     3301 aggcatcggg ctcaccgatc acggcccgcg actcgctcgc cgagaacgtc cgcagcgcaa
     3361 tgcgatacga caggtggctg tcgagcccgc ggagccggcc ctcctcgagc cgctgtgagg
     3421 cgagcagcag gtggatcccg agcgaccgcc cgagccggcc catggcgacg aagagctcgg
     3481 tgaactcggg ctgggccgtg agcagctcgg tgaactcgtc gcagacgatg acgagggtcg
     3541 gcaaggggtc gagggcccgc ccggcggacc gcgcggcgtt gtagtcgcgc agcgaggcga
     3601 cgttgccggc agcccggagc acctgctggc ggcgcagcat ctcgccgctg agcgcgtcgt
     3661 acatccggtc gacgagggtg agctcctcgc gcaggttggt gatcaccgcc gagacgtgcg
     3721 gcatccctgc gagcccggcg aaggtggccc cgcccttgaa gtcgacgagg acgaggttga
     3781 ggtcctcggg ggaatgggtc agcgcgagcc ccacgacgac cgtgcgcaac agctcggact
     3841 tgccggatcc cgttgcgccg atgaggagcc cgtgcggacc catgccgtgc tcggcggctt
     3901 ccttgaggtc gagccagacc ggtgcccccg cgtcgtcgac gccgatcggc acccgcagcc
     3961 ggtcgcgggc tccccgcggg gcccaggcgc gggacaggcg cagccggcgc aggtccccga
     4021 gcccgagcag gtcgacgagc tcggggctca caccgtcccc ggtcctcccc cgggggcgct
     4081 cgtcccggcc ccaggccgcg aggcgccggg cgagagcgtc ggctccggcg atggacagct
     4141 ggtccgccca cccgtcgacc ggctcacccc cagccggtat gacgcgcagg ctcacctcgc
     4201 ccgcggcgcc cccggctgag tccctcccgg tccccagcac gagcaccggg gccacgtcgc
     4261 tcgccggcgg aggcgtcccc ccgatcgtga ggaccgtcat accgggcagg tc
//
LOCUS       DATAIX010000739         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_144558, whole genome shotgun sequence.
ACCESSION   DATAIX010000739 DATAIX010000000
VERSION     DATAIX010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144558"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..528
                     /locus_tag="VHM65_10940"
     CDS             232..528
                     /locus_tag="VHM65_10940"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HEX2706265.1"
                     /translation="MGRVLMFVVPLGLTIYALVDCLQTDESAVRSIPKLWWIMLIVLV
                     WVVGPVAWLIAGRPQSILPPSLGGPQRGQRPGPGHLRDNRPRGPEDDPDFLRGL"
     gene            595..1503
                     /locus_tag="VHM65_10945"
     CDS             595..1503
                     /locus_tag="VHM65_10945"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:NF004751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEX2706266.1"
                     /translation="MATLAQWVAGARPRTLPAALSPVLVGVGATVADVGRWSPVRAVL
                     ALVVAVALQVGVNYANDYADGIRGTDRERVGPVRLVGQGLARPRQVKAAALASFAVAC
                     LAGLGLAALSASWWLVAAGAACVLAAWGYTGGRSPYGYRGLGEVFVFVFFGLVATLGT
                     QYTQYARALGSPHVTAAGFLGAIGVGALACAILVANNLRDIPTDTLAGKHTLAVRLGD
                     RGTRRLYVALVTAAALAVLGCAVVEGPRSALGLVAFALAARPLRALTTGATGRDLVPV
                     LAGTGVLQLGYALLLTLGLTLTGPSA"
BASE COUNT          188 a          680 c          591 g          256 t
ORIGIN      
        1 cagctcgctc accgtgggtg ggcgagctgc gcacgacgct gcgcggcata ctcgccgacc
       61 atgccctgcc ccggcgggtg gtgacgctgt ccgacatacc acggcggggt cccggcaagc
      121 ccgaccgcgc ggcggttcgg cggctggtcg cgcccgaacc gtgaccgacc cctggcgttc
      181 gccgtgctgg ggcggtggga caatcagggc gagtcgagca aaggtggagc cgtgggtcgt
      241 gtcctgatgt ttgtcgtgcc gctcgggctg acgatctacg cgctcgtcga ctgcctgcag
      301 actgacgagt cggcggtccg cagcatcccc aagctgtggt ggatcatgct catcgtcctc
      361 gtctgggtgg tcgggccggt ggcctggctc atcgccgggc ggccacaaag catcctgccg
      421 ccgagcctcg gtggcccgca gcggggccag cggcccggtc ccggccacct gcgcgacaac
      481 cgcccccgcg ggcccgagga cgaccccgac ttcctgcgcg gtctctagac ccagccccca
      541 ctggccgcct gcccgacctg ccttgccttg cccacgagga gaccgcacct cccaatggcc
      601 actctcgccc agtgggtcgc cggcgcccgc ccacggacct tgcccgccgc gctctcgccc
      661 gtgctcgtcg gggtcggggc gacggtcgcc gacgtcggca ggtggtcgcc ggtacgggcc
      721 gtgctggcgc tcgtcgtcgc ggtggcgctg caggtcggcg tcaactatgc caacgactac
      781 gccgacggca tccgcggcac cgaccgcgag cgggtcggcc cggtccgcct cgtcgggcag
      841 gggctggcgc ggccacgcca ggtcaaggcc gccgcgctgg cgtcgttcgc ggtggcgtgc
      901 ctcgccggtc tcgggctcgc cgcgctgagc gcctcgtggt ggctcgtcgc cgccggagcg
      961 gcgtgcgtcc tggccgcctg gggctacacc ggtgggcgca gcccctatgg ctaccgcggc
     1021 ctcggcgagg tcttcgtctt cgtcttcttc ggcctggtcg ccaccctcgg cacgcagtac
     1081 acccagtacg cccgtgcgct cggatccccc cacgtcaccg ccgcgggctt cctcggagcg
     1141 atcggcgtcg gagccctggc ctgcgcgatc ctcgtcgcca acaacctgcg cgacatcccc
     1201 acggacaccc tggcgggcaa gcacacgctc gccgtccgcc tcggagaccg tgggaccagg
     1261 cgcctgtatg tcgccctggt cacggccgcc gcgctcgcgg tgctcgggtg cgcggtggtc
     1321 gagggaccaa ggtccgcgct cggcctggtc gccttcgccc tggccgctcg cccgctgcga
     1381 gccctgacca cgggcgccac cggtcgcgac ctcgtccccg tcctggccgg caccggagtc
     1441 ctccagctcg gctatgccct gctcctcacc ctcggcctga ccctcaccgg gccctcggcc
     1501 tgaccctcac cgggccctcg gcctgaccct caccgggccg tggccgggcc gtcgcacccg
     1561 cgcgccgctg agtaactcct cgcgatgcaa atcaccgacg cggggggcca gtatccggcg
     1621 cgaggatgcc ccccggagcg agcgctcgaa ccttccgggc ccgcgctcgt agaggtcatc
     1681 caccacgtcc cgggcggcag ccggcctggc aggtt
//
LOCUS       DATAIX010000740         3242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_265107, whole genome shotgun sequence.
ACCESSION   DATAIX010000740 DATAIX010000000
VERSION     DATAIX010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265107"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..422)
                     /locus_tag="VHM65_10950"
     CDS             complement(33..422)
                     /locus_tag="VHM65_10950"
                     /inference="COORDINATES: protein motif:HMM:NF039800.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HEX2706267.1"
                     /translation="MRRYCEPIEVLPDEHGQPVRFIWHGRLYLVLAVLGHWRERRAWW
                     REVLDPVLDPVPTRRGGAPDDGAAADGAPSSVATVERALDPTERLVWRVEASVGRLGN
                     AGVYDLALEPAGLPGGPGQWQLLRVSD"
     gene            660..1187
                     /locus_tag="VHM65_10955"
     CDS             660..1187
                     /locus_tag="VHM65_10955"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX2706268.1"
                     /translation="MPDARHTSPGSDPIRSHPNVQRVRSALDALGVHTEITVLDGAAR
                     TAAQAADFLGIIVGQIASSLVFTAHSHDEVAPTPLLVLTSGAHRVDTTTLAASLGLAS
                     IGRADAELVRASTGFVIGGVPPVGHLAPVRTLVDVALAGYDTIWAAAGHPHTVFETTY
                     DELVRITGGQPAEVG"
     gene            1336..1956
                     /locus_tag="VHM65_10960"
     CDS             1336..1956
                     /locus_tag="VHM65_10960"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX2706269.1"
                     /translation="MGAEALPWVFTSGWASGINAYAVVLIMGIAERFFGVAQVPAALG
                     RTDVLVGAAVMFVLEVFADKIPYLDTIWDSVHTVVRPAVGATIGYLIGDQSSDLDAAF
                     MAATGGISALVSHLVKAGIRTGVNTSPEPVSNVAVSTGEDVAVAGVVSLAFVNPWLAA
                     GIATALLAVGVALVLLILRRFRGLKRRYDEWGKQAGIAAPLPDDHT"
     gene            complement(2040..2984)
                     /gene="metF"
                     /locus_tag="VHM65_10965"
     CDS             complement(2040..2984)
                     /gene="metF"
                     /locus_tag="VHM65_10965"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEX2706270.1"
                     /translation="MTHGTATSVHRPDHVTRSIPSLVAAPAPTFSFEFFPPKDDAGAE
                     ALWAAIRRLEQVGPSFVSVTYGAGGATQERTVRMTERIAADTTLTPLGHLTCVGASVA
                     TLRNVVGLYAAAGVHNILALRGDPPGGLGTPWVAHPEGLNHAEQLVALIKELGDFTVG
                     VAAFPDRHPESPSLERDAEILVRKADAGAEYAITQFVFDVDSYLRLRDRVGALGRDLP
                     IVAALMPVTNIAQLKRMAELCGQPLPAAVVGRLEAVAEDKAAVVREGVAICTESAQRL
                     LAEGAPGIHFITMNRSPATLEVFQNLGLAPLRPRAHPG"
BASE COUNT          466 a         1233 c         1082 g          461 t
ORIGIN      
        1 gggccgggct gtggtcgtca tggtcgcctc tcctagtcgc tcactcgcag cagctgccac
       61 tggccggggc ccccgggcag cccggcgggc tccagggcca ggtcgtagac cccggcgttg
      121 cccagccggc ccacgctcgc ctcgacccgc cagacgagcc gctcggtcgg gtcgagggcc
      181 cgctcgaccg tcgccacaga ggacggcgcg ccgtcggccg cagcaccgtc atccggcgcc
      241 ccgccacggc gggtcggcac cgggtcgagc accgggtcga gcacctcacg ccaccacgcc
      301 cgccgctcgc gccagtggcc gagcacggcc aggacgaggt agagccgtcc atgccagatg
      361 aacctcacgg gctggccgtg ctcatccggc aggacctcga tcggctcgca gtatcggcgc
      421 accacgacat ggcccctctc gcttcgggct ggacgaactg atggagtcgg gccgccctgc
      481 ggtgtgcggg gtcgagtcgc acaccgcagg tcagcccccg gctcgaacat gtcgtacacc
      541 tgttcgatag aacgaatgta ctactgccca ccgacaggct gtcaaggccg gtccgcccgc
      601 gcacgtccgg gccgcgggac cgtacgcccc ggcaggacct catcggtacc ctcgtgcgca
      661 tgcccgacgc gcgccacacc agccccggca gtgacccgat ccgctcccac ccgaacgtcc
      721 agcgcgtccg gtcggccctc gacgcgctcg gcgtccacac cgagatcacc gtcctcgacg
      781 gggccgcccg caccgccgcc caggcggccg acttcctcgg catcatcgtc gggcagatcg
      841 ccagctccct tgtcttcaca gcgcattcgc atgacgaggt cgcgcccact cctctgcttg
      901 tcctcacctc cggcgcccac cgggtcgaca ccaccacgct cgcggccagc ctcgggctgg
      961 cttcgatcgg ccgagccgac gccgagctcg tccgcgcgag caccgggttc gtcatcggcg
     1021 gtgtccctcc ggtcgggcac ctcgcgccgg tccgcacgct cgtcgacgtc gcgctcgccg
     1081 gctacgacac catctgggcc gctgccggcc acccgcacac cgtgttcgag acgacgtatg
     1141 acgagctcgt ccggatcacc ggtggccagc ccgccgaggt gggctagcgc ggcgggtctc
     1201 acgacgtccg cccggcatct cgcgctaccg acgattcctc ttcctaacgg ttgcgcagcg
     1261 gggaatggca ccccggaccg cacgctgaca tcctgcaggg ggacgcaccg agcgagccct
     1321 gccggaagga cgccaatggg tgcagaagct ctcccgtggg tcttcacctc cgggtgggcc
     1381 agcgggatca acgcctatgc cgtcgtgctc atcatgggga tcgccgaacg cttcttcggc
     1441 gtcgcccagg tgcccgctgc cctcgggcgc accgacgtcc tcgtcggcgc cgcggtgatg
     1501 ttcgtcctcg aggtgttcgc ggacaagatc ccctacctcg acacgatctg ggacagcgtc
     1561 cacacggtgg tccgcccggc cgtcggggcc accatcggct acctcatcgg tgaccagagc
     1621 tcggacctcg acgcggcctt catggcggcg accggcggta tcagcgccct cgtctcgcac
     1681 ctggtcaagg ccgggatccg caccggggtc aacacctccc ccgagcccgt gagcaacgtc
     1741 gcggtgagca ccggcgagga tgtcgcagtt gccggagtcg tctcgctcgc cttcgtcaac
     1801 ccctggctcg ccgccgggat cgcaaccgcg ctgctcgcgg taggagtcgc gctggtcctc
     1861 ctcatcctgc ggcggttccg cgggctcaag cgccggtatg acgagtgggg caagcaggcg
     1921 ggtatcgccg cgccgctccc ggacgaccac acctgatgca cccggcgtca cctcacccgg
     1981 cgtcacctca cccggggtga gctcgttgcg gtccagccac cgccaccacc gcaagcacgt
     2041 caccctgggt gagctcgcgg gcgcagaggg gccaggccga ggttctggaa gacctcgagc
     2101 gtcgccggtg accggttcat cgtgatgaag tggatgccgg gagcgccctc ggcgagcagc
     2161 cgctgggcgg actcggtgca gatcgccaca ccttcgcgca cgaccgcggc cttgtcctcg
     2221 gccaccgcct cgagccggcc gaccaccgcg gcgggcaggg gctgtccgca gagctcggcc
     2281 atccgcttga gctgcgcgat gttggtcacc ggcatgagcg cggcgacgat cggcaggtcg
     2341 cggccgagcg cgccgacccg gtcgcgcagc cgcaggtagg agtcgacgtc gaagacgaac
     2401 tgggtgatcg cgtactcggc gcccgcatcg gccttgcgga cgagtatctc ggcgtcgcgc
     2461 tccaggctcg gcgactccgg gtgccggtcc gggaaggccg cgacgccgac cgtgaagtca
     2521 ccgagctcct tgatgagggc gacgagctgc tcggcatggt tgagcccctc gggatgggca
     2581 acccacggcg tgccgagccc gcccggcggg tcaccacgca aggcgaggat gttgtggacc
     2641 ccggctgcgg cgtacaggcc gacgacgttg cgcagcgtgg cgaccgaggc tcccacgcag
     2701 gtgaggtgcc cgagcggcgt caaggtggtg tccgcggcga tccgctcggt catccgcacc
     2761 gtgcgttcct gggtcgcgcc acccgcgcca tacgtcaccg acacaaagct cgggccgacc
     2821 tgctccaggc gccggatcgc cgcccacagt gcctcggcgc cggcgtcgtc cttcggcggg
     2881 aagaactcga agctgaaggt gggcgcgggt gcggccacca gcgacgggat cgagcgagtc
     2941 acatgatccg gtcgatggac gctcgtcgcg gtgccatggg tcatgaggcc agggtacggg
     3001 cgcccgaagg gccgcggccg aacgactagg ctcgggtgag accgtctgcg ccacctctcg
     3061 acacccttgc cctatccaca ccctgcccta tccacaccct tgccctcaca acaccctgcg
     3121 acgcccgacc cggcggagcc tgtgcccagc ccgctcgcgc gtgccgacct gctcacccgg
     3181 gtccagcgcg tcgtggacag cgagctcacc aggcaagggc aggtgctcgc gcagatcggt
     3241 cc
//
LOCUS       DATAIX010000741         1825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_145074, whole genome shotgun sequence.
ACCESSION   DATAIX010000741 DATAIX010000000
VERSION     DATAIX010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145074"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..>1825
                     /gene="acnA"
                     /locus_tag="VHM65_10970"
     CDS             46..>1825
                     /gene="acnA"
                     /locus_tag="VHM65_10970"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX2706271.1"
                     /translation="MGSVDSFGAKGALVVGGTSYEVYRVGAVEGSATLPFCLKVLLEN
                     LLRTEDGANVTAEQIRALGSWVAGAEPGTEIQFTPARVLMQDFTGVPCVVDLATMREA
                     VAALGGDAAKINPLAPAEMVIDHSVIVDVFGRADAFERNMELEYERNRERYQFLRWGQ
                     SAFDEFRVVPPGTGIVHQVNLEHLARTVMVRGGVAYPDTCVGTDSHTTMVNGLGVLGW
                     GVGGIEAEAAMLGQPVSMLIPRVVGFKLSGSIPAGATATDVVLTITQQLRRHGVVGKF
                     VEFYGEGVGAVPLANRATIGNMSPEFGSTCAIFPIDDVTLDYLRLTGRSPESVALVEA
                     YAKEQGLWHDPSTEARFSEYLQLDLSTVVPSIAGPKRPQDRIGLVDAKSQFAADVQNY
                     GAAGGLRSVPVSRGGATFELRDGAVVIASITSCTNTSNPSVMMAAALLAKKAVEKGLS
                     VQPWVKTSMAPGSKVVSDYYEKAGVWPYLDKLGFNLVGYGCTTCIGNSGPLPAEVSAG
                     VQSGDLAVAAVLSGNRNFEGRINPDVTMNYLASPPLVIAYALAGTMDFDPESDPLGTG
                     SDGAPVYLRDLWPTPAQVEAVVARAIT"
BASE COUNT          266 a          636 c          637 g          286 t
ORIGIN      
        1 gttagatatc ttgacgtcga gatacatggg tgagaggagc ccgtcgtggg cagtgtggac
       61 agcttcggag ccaagggcgc gctcgtggtg ggcgggacgt catacgaggt ctatcgggtg
      121 ggggccgtcg agggcagcgc gacactgccg ttctgcctca aggtgctcct ggagaacctg
      181 ctgcgcaccg aggacggcgc caacgtcaca gccgagcaga tccgggcgct gggctcctgg
      241 gtggccggcg ccgagcccgg caccgagatc cagttcaccc ccgcccgggt cctcatgcag
      301 gacttcaccg gggtgccgtg cgtcgtcgac ctcgcgacca tgcgcgaggc cgtggccgcg
      361 ctgggcggcg acgccgcgaa gatcaacccg ctcgcgccgg ccgagatggt catcgaccac
      421 tcggtgatcg tcgacgtctt cggccgcgcg gacgccttcg agcgcaacat ggagctcgag
      481 tacgagcgca accgggagcg ctaccagttc ctgcgctggg ggcagagcgc gttcgacgag
      541 ttcagggtcg tcccgcccgg cacgggcatc gtccaccagg tcaacctcga gcatctcgcc
      601 cgcacggtca tggtgcgtgg gggagtggcc taccccgaca cctgcgtcgg caccgactcg
      661 cacacgacga tggtcaacgg cctcggggtg ctcggctggg gcgtcggggg gatcgaggcc
      721 gaggccgcca tgctcggtca gccggtgtcg atgctcatcc cgcgggtcgt cggcttcaag
      781 ctgtccgggt cgatcccggc cggcgcgacg gcgaccgacg tcgtcctgac catcacccag
      841 cagctgcgcc ggcacggtgt cgtgggcaag ttcgtcgagt tctacggcga gggcgtcggt
      901 gcggtgccgc tggccaaccg cgcgacgatc ggcaacatga gcccggagtt cggctcgacc
      961 tgcgcgatct tcccgatcga cgacgtcacc ctggactacc tgcgcctgac cggtcgctcg
     1021 cccgagtcgg tggcgctcgt cgaggcctat gccaaggagc aggggctctg gcacgacccg
     1081 tcgaccgagg cgcgcttctc ggagtacctc cagctcgacc tgtcgaccgt ggtgccttcg
     1141 attgccgggc ccaaacgccc tcaggacagg atcgggctgg tcgacgccaa gtcccagttc
     1201 gcggcggacg tgcagaacta cggcgcggcc ggcgggctcc ggtcggtccc ggtctcgcgc
     1261 ggcggggcga ccttcgagct gcgggacggc gccgtcgtca tcgcctcgat cacgtcgtgc
     1321 accaacacct cgaacccctc ggtcatgatg gccgccgcgc tgctggccaa gaaggccgtc
     1381 gagaagggcc tgtcggtgca gccgtgggtc aagacctcga tggcgcccgg gtcgaaggtc
     1441 gtctcggact actacgagaa ggccggcgtc tggccctacc tcgacaagct cggcttcaac
     1501 ctcgtcggct acggctgcac gacgtgcatc ggcaactccg gtccgctgcc cgccgaggtg
     1561 tcggccgggg tccagtccgg tgacctcgcg gtggcggcgg tcctctcggg caaccgcaac
     1621 ttcgagggcc ggatcaaccc cgacgtcacg atgaactacc tcgcgtctcc acctctcgtc
     1681 atcgcctacg ccctggccgg cacgatggac ttcgaccccg agtccgaccc gctgggcacc
     1741 ggttcggacg gcgcaccggt ctacctgcgc gacttgtggc cgaccccggc gcaggtcgag
     1801 gcggtggtcg cccgggcgat caccc
//
LOCUS       DATAIX010000742         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_145218, whole genome shotgun sequence.
ACCESSION   DATAIX010000742 DATAIX010000000
VERSION     DATAIX010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145218"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1492)
                     /locus_tag="VHM65_10975"
     CDS             complement(<1..1492)
                     /locus_tag="VHM65_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX2706272.1"
                     /translation="MTPPATAQSRQTVRLSVAQATIRFLASQWSERDGERRRLFAGVF
                     GIFGHGNVAGIGQALLEHELEATTAGSPPALPYVLARNEQAMVHTSVAYARQQDRLQT
                     WVCTASVGPGSTNMLTGAALATINRLPVLLLPSGTFATRCGGPVLQELEPAYAGDLTV
                     NDAFRPLSRFFDEVNRPEQRRGQLLGAMRVLTDPAETGAVTISLPEDVQAEPFDWPVE
                     LFAPRVWRVPRPLPQPVDIADAAAVIRSARRPMVVAGGGVHYSGAEAALEAFCAATGI
                     PVGEAQAGKGSLPHGHPQQMGGVGSTGTTAANALAHDADVVIGIGTRYSDFTTGSRTA
                     FQDSGVRFVNINVARFDAAKHSGLMVVSDAREALTALTAALDGWSVVPAYRARQGELW
                     ASWDAEVEAAYHPPVEVTSALARGLLTQGQVLGAVNELTGPRDVVLCAAGSMPGDLHK
                     LWRVRERKAYHVEYGYSCMGYEIPAALGVRLADETRDVFAMVGDGGY"
     gene            complement(1492..2406)
                     /gene="iolB"
                     /locus_tag="VHM65_10980"
     CDS             complement(1492..2406)
                     /gene="iolB"
                     /locus_tag="VHM65_10980"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEX2706273.1"
                     /translation="MRADRQWVRPSGSCAQGGYDVAIVPGSPGWAHTGLYVVNLAAGR
                     SHSMPTAGTEVIVVPLSGALDVLAVDGSGARHEASLAGRVSVFAGPTDIAYVPPGATL
                     TLTSADGARVALPFAPVAKPGEPAPFRHLAAGEVPVELRGAGRATREVRSFGVPGVLD
                     ADSIIACEVVTPAGNWSSYPPHKHDTERDGAETALEEIYYFQIRPDGSQAGPGADPVG
                     YQRVYGTPERPIDVLAEVRSGDVVLVPYGWHGPAMAPPGYDMYYLNVMAGPGPVRAWL
                     ICDDPAHAWVRDTWPGQSPDPRLPMGGQ"
BASE COUNT          359 a          919 c          949 g          319 t
ORIGIN      
        1 ggtagccacc gtcgccgacc atggcgaaca cgtcacgggt ctcgtcggcg agccggaccc
       61 cgagcgcggc ggggatctcg tagcccatgc aggagtagcc gtactcgacg tggtaggcct
      121 tgcgctcacg cacccgccac agcttgtgca ggtcgccggg catcgacccg gccgcgcaga
      181 ggacgacatc gcgcgggccg gtgagctcgt tgaccgcccc gaggacctgc ccctgggtga
      241 gcagcccccg agcgagcgcg ctggtcacct ccaccggcgg gtggtatgcc gcctccacct
      301 cggcgtccca gctcgcccag agctcgccct ggcgcgcgcg gtaggccggg acgaccgacc
      361 acccgtcgag ggccgccgtc aatgcggtga gcgcctcacg ggcgtcggag acgaccatga
      421 gcccgctgtg cttggccgcg tcgaagcggg caacgttgat gttgacgaag cgaaccccgc
      481 tgtcctggaa cgccgttcgc gacccggtgg tgaagtcgct gtagcgggtg ccgatgccga
      541 tgacgacgtc ggcgtcgtgc gcgagggcgt tggccgccgt ggtccccgtc gagcccacgc
      601 cgcccatctg ctgggggtga ccgtgcggga gcgagccctt gcccgcctgc gcctcgccca
      661 ccgggatccc ggtcgcggcg cagaacgcct ccagcgccgc ctcggctccg gagtagtgca
      721 ccccgccacc ggccacgacc atgggccggc gcgccgagcg gatgaccgcg gcggcgtcgg
      781 cgatgtcgac cggctggggc agcgggcgcg gtacgcgcca tacgcgggga gcgaagagct
      841 cgaccggcca gtcgaacggc tcggcctgga cgtcctccgg cagcgagatc gtcacggcgc
      901 ccgtctcagc cgggtcggtc agcacccgca tcgccccgag cagctgccct cggcgctgct
      961 cgggccggtt gacctcgtcg aagaagcgcg acaggggacg gaacgcgtcg ttgacggtga
     1021 ggtcaccggc atacgccggc tcgagctcct ggagcaccgg gccgccgcac cgcgtcgcga
     1081 acgtccccga cggcaggagg agcacgggca gccggttgat cgtcgcgagg gccgccccgg
     1141 tcagcatgtt ggtcgagccg gggccgaccg aggccgtgca gacccaggtc tggaggcggt
     1201 cctgctggcg ggcataggcg acggaggtgt ggaccatggc ctgctcgttg cgggccagga
     1261 cataggggag tgcgggcggg ctcccggccg tcgtcgcctc gagctcgtgc tcgagcaggg
     1321 cctggccgat cccggcgacg ttgccgtgcc cgaagatgcc gaacaccccg gcgaagagcc
     1381 gccgccgctc accgtcgcgc tcgctccact ggctcgcgag gaaccggatc gtcgcctggg
     1441 cgacggagag gcgcacggtc tggcgggact gggccgttgc cggcggcgtc atcactggcc
     1501 tcccatgggc agccggggat cgggggactg gccgggccac gtgtcgcgca cccaggcatg
     1561 ggccgggtcg tcgcagatga gccaggcgcg gacgggaccg gggccggcca tgacgttgag
     1621 gtagtacatg tcgtagccgg gcggggccat ggccggcccg tgccagccgt aggggacgag
     1681 gacgacatcc cccgaccgga cctcggcgag gacgtcgatc gggcgctccg gcgtcccgta
     1741 gacccgctgg tagccgacgg gatccgctcc agggccggcc tgcgagccgt ccgggcggat
     1801 ctggaagtag tagatctcct cgagcgccgt ctcggccccg tcgcgctcgg tgtcgtgctt
     1861 gtgcggaggg taggacgacc agttgccggc cggggtcacc acctcgcacg cgatgatcga
     1921 gtccgcgtcg aggacgccgg gcaccccgaa gctgcgcacc tcacgggtgg cccgcccggc
     1981 gccccgcagc tcgaccggca cctcgcccgc cgccaggtgc cggaaggggg caggctcacc
     2041 gggcttggcg accggtgcga acggcagtgc caccctggcc ccgtcggccg acgtcagcgt
     2101 cagcgtggca ccggggggca cataggcgat atcggtcggc ccggcgaaga ccgacacccg
     2161 gccggccaga gacgcctcat gccgggcccc cgagccgtcc acggcgagca cgtcgagcgc
     2221 cccggacaac ggcaccacga tgacctcggt ccccgccgtg ggcatcgaat ggctccgccc
     2281 cgcggcgagg ttcacgacat acagaccggt atgtgcccag cccggggacc cggggacgat
     2341 cgcgacgtca tacccgcctt gagcgcagga gccactcggg cggacccact gccggtctgc
     2401 tctcacgccg tgtctccggg cgccggcgcc gtggtggaca gggccgagag cacctcgtcc
     2461 tcggtgggca tcgccgtgga gcactccagc cgcgaggcga cgatcgcgcc ggcgacattg
     2521 gcgaaacgca gcgtccgctc cagggg
//
LOCUS       DATAIX010000743         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_265268, whole genome shotgun sequence.
ACCESSION   DATAIX010000743 DATAIX010000000
VERSION     DATAIX010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265268"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..404)
                     /locus_tag="VHM65_10985"
     CDS             complement(117..404)
                     /locus_tag="VHM65_10985"
                     /inference="COORDINATES: protein motif:HMM:NF014520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX2706274.1"
                     /translation="MQLTAVIDYGDQALLSGADIPCRELDAELWFAERPDDVEHAKAL
                     CRTCPVRAECLAGALDRSEPWGVWGGELLVQGVIVPRKRPRGRPRKNDVAA"
     gene            complement(576..770)
                     /locus_tag="VHM65_10990"
     CDS             complement(576..770)
                     /locus_tag="VHM65_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706275.1"
                     /translation="MKKTNTTGMTMTGWTTYASLRPRGYAHLVSVTTPVQGTGLSIAS
                     TDALPTTADVEYAPTWSPPL"
     gene            complement(930..>2204)
                     /locus_tag="VHM65_10995"
     CDS             complement(930..>2204)
                     /locus_tag="VHM65_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HEX2706276.1"
                     /translation="VRNYRSTPQIVGLANTVMRSPRSAGATAGSVELVAQSAGGAAPT
                     LTGYADDPAEADGVAAAIAGLVQAGRRPAEIAVLFRTNAQSEAVESALSQAGVPYLVR
                     GGERFFARKEVRAAVLLLRGAARSDDGERPLGELVRDVLGGAGWTEAPPSAAGAVRER
                     WESLAALAALADDLVATTAQARLPDLVRELDERAAASHAPAVQGVTLASLHAAKGLEW
                     DVVFVVGCSDGFIPITMADTSEAILEERRLLYVGLTRARHELRLSWGAARTPGARASR
                     RPSRFLDVAAGILGEGARSTAKSPASVRGGAAPKVAKVAKVAKRTTCRSCGVDLVTAA
                     ARKVGRCADCPPTYDPATFEALRSWRLDTAREASVPAYVIFTDATLTVIAERTPASLT
                     ELASISGVGARKLDQYGAAVLGVLHGSGDEKP"
BASE COUNT          299 a          780 c          806 g          319 t
ORIGIN      
        1 tgacgaaact ccttgtgaac tgcggtgatt gacaccgcga gagatgaggg tcggatcact
       61 ggtgctgacc cggcagccgg ttcgttgccc cgaccggctg ccgggagatc gacgagtcag
      121 gccgcgacgt cgttcttgcg cggcctgccg cggggtcgct tgcgcgggac gatgacgccc
      181 tgcacgagca gctcgccgcc ccagacgccc cagggctcgg agcggtcgag ggcaccggcg
      241 aggcactccg cgcgcaccgg gcaggtgcgg cacagcgcct tggcgtgctc gacgtcgtcg
      301 gggcgctcgg cgaaccagag ctcggcgtcg agctcccggc acgggatgtc ggcacccgaa
      361 agcagggcct ggtcgccgta gtcgatcact gcagtgagct gcatcaggtg tcacctcctt
      421 cgtcgcggcc gcggtggcgg cctggctggt ccttccggac ttctggtcct tctggttctg
      481 gtcgcgctgc ctgcagaatc cgggcggaca aacgaaaagg ccgcggatcc cggatgtcgg
      541 gttccgcggc ctggagggcc tgctgcctgt ggcggctaca ggggtggact ccaggtcggg
      601 gcatactcga cgtcggcggt cgtggggagg gcatcggtgc tggcgatgga cagacctgtc
      661 ccctggacgg gggtggtgac cgagacaagg tgggcgtagc cgcgcggacg cagcgaggcg
      721 taggtcgtcc aaccggtcat ggtcatcccc gtggtgttcg tcttcttcat cgtggccacc
      781 ctcctctcgc ttcctcggca ctcttcgcgc ccggcgtatg ccgcttcggt caggttacgc
      841 cgggcgcggt tcccgtcaca agcgaataat cgagaaagtc gcagggaagc tctgcgcagg
      901 gggcctgagc cagccagctc gcccatcctt cacggctttt cgtcgcctga cccgtggagg
      961 acaccgagga cggcggcgcc gtactggtcg agcttgcgcg ccccgacccc gctgatcgag
     1021 gccagctcgg tgaggctcgc cggggtgcgt tcggcaatga cggtgagcgt cgcgtcggtg
     1081 aagatgacgt atgccggcac gctcgcctcg cgggcggtgt cgaggcgcca ggagcgaagg
     1141 gcctcgaacg tcgccggatc gtaggtcggc gggcagtcgg cacaccggcc caccttgcgc
     1201 gcagcggcgg tgacgaggtc caccccgcac gagcggcagg tcgtgcgctt ggccaccttg
     1261 gcgaccttgg ccaccttggg cgccgcgccc ccacggacgc tcgccggcga cttcgccgtg
     1321 ctgcgcgccc cctcgccgag gatccccgcc gcgacgtcga ggaagcgtga gggacgcctg
     1381 ctcgcccgag caccaggggt gcgcgcagcc ccccacgaca ggcgcagctc gtggcgtgcc
     1441 cgcgtcagac cgacatacag gaggcggcgt tcctcgagga tcgcctcgga ggtgtcggcc
     1501 atggtgatcg ggatgaagcc gtcgctgcac ccgacgacga agacgacatc ccactccagg
     1561 cccttggccg cgtgcagcga ggccagcgtg actccctgga cggcgggggc gtgcgaggcc
     1621 gccgcccgct cgtccagctc gcggacgagg tcgggcagcc gcgcctgcgc cgtcgtggcg
     1681 acaaggtcgt cggccagggc cgcgagcgcc gcgagcgact cccagcgctc gcgcaccgcc
     1741 cccgcggcac tgggcggggc ctcggtccac ccggctcccc cgaggacgtc gcggacgagc
     1801 tcgcccagcg gcctctcgcc gtcgtccgac cgtgcggcac cccgaaggag cagcaccgcg
     1861 gcccgcacct ccttgcgcgc gaagaaccgc tcaccgccgc ggacgaggta gggcacgccg
     1921 gcctgggaca gcgcggactc gaccgcctca gactgggcgt tggtccggaa gagcacggcg
     1981 atctcggccg gacgccgtcc cgcctggacg agcccggcga tcgcggcagc caccccgtcg
     2041 gcttcggccg ggtcgtcggc gtacccggtc agggtcggcg cggcaccccc ggcgctttgg
     2101 gccacgagct cgacactgcc cgccgtcgcc ccagccgagc gtgggctgcg catgacggtg
     2161 ttggccagcc cgacgatctg cggggtcgat cggtagttgc gcac
//
LOCUS       DATAIX010000744         2648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_265319, whole genome shotgun sequence.
ACCESSION   DATAIX010000744 DATAIX010000000
VERSION     DATAIX010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265319"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1217..2005
                     /locus_tag="VHM65_11000"
     CDS             1217..2005
                     /locus_tag="VHM65_11000"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX2706277.1"
                     /translation="MRPARDYAYFDAPPLAFAHRGGALYEPNLHRENTLVAFRNAVEL
                     GYRYIETDVHATSDGVLVAFHDKELDRVTDGRGAISGLPYAQVREARIAGQEPIPLLA
                     EILEALPATRVNIDIKASGAIGPLWRAIEEHSAHDRVCVASFSNLRLARFRRLARGRV
                     ATAAGWAGTAALRFGPAALTAVVSSPGQVLQIPDRMPLAGRPVEVVTPRLIETAHRVG
                     KQVHVWTIDDPGDMTRLLDMGADGIVSDRIEVLRRVLEARGQWA"
     gene            1996..>2648
                     /locus_tag="VHM65_11005"
     CDS             1996..>2648
                     /locus_tag="VHM65_11005"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2706278.1"
                     /translation="MGLSPSETDRFPPEGPFAPAYRLISLALVALVSIVAFEFMAITT
                     AMPAAAQELGAVRSYGLAFSVMITLQVLGIVLAGAWSDLRGPMPAMYAGQLLFAAGAT
                     VCALAASFPVFLLGRAVTGLGAGLIVVAEYVIIGRVYPPALRPRFFTIVSAGWVLPSL
                     VGPPISAWLTTSFSWRWVFGIVAVPALVTFTVIASQAGRVRAGPTPPEQPDQGEPPG"
BASE COUNT          367 a          967 c          917 g          397 t
ORIGIN      
        1 ccctgtgcgt ggtgaacctc ggggccgagc cggtcacgct ccctgcccac gccgagctca
       61 tcgccgcatc cggggacctc gtggacggcc gggtgcccct cgatacggcg atctgggccc
      121 gcctgagctg acccgtggca cccgtggcac ccccacgtcg gcggcccggg gcccgacctg
      181 ctcccggttt cgagtcgata gccgataggc gacttttcgt ccgtcacgtc actcctgcca
      241 cccctgtgcc agacgacacg gcagcctcag tctcagagcg cctctgcgag ctcgggctgt
      301 gcggggctca ggaccgggcc aggcgggggg accgagtgat gatgatcgcg ctgcagacca
      361 tggtctcgtg gttctcgggc agggtttcag tggtcgcgga tgcagcggcg ctgggtggtc
      421 cagccgcagg tgccttccat gacccagcgg cggggatgac gacgaacccc gtggcgtcgt
      481 cggttccttt ggcgaccgca atggcgagga agggcacggt ttgggctcag cggaccggtc
      541 ggccgcggtg accggggccg actcagaccc aggtgatggc ggagaacctg gcgggcagct
      601 ggaccaggag ccggcgcgtg ccgtgacgat cctggaggct gggcgggggg tccgcgaccg
      661 ccggccccag acgagcgagg tggatctgac cggtgccgac cggccggatc gacctcattc
      721 cgccaccccg gacgagcgag gtggatctga ccggtgccga ccggccggac ccacctcagc
      781 gatgagggca cgatgagctc cagatgggta cggtctcgac aaccaccgac ccaccatgag
      841 ctcacccact caccacaggc accaggcaac acgcgccccg ccagcaccag agctcccagc
      901 cgcgctctca ccgtgacggg ctcgtgcggt ggcacgctac ccccccgcaa ccacgtcacc
      961 ctcggtgacc cggctgggat acccacggcc gccagccgcg accacgtcac ccacggtgac
     1021 gtggtcgcgc tgagctgccc ggcgatatgt ggtgcgggtg gtgcgggttg tgcacgtggt
     1081 gcacgcggta cggaatatcg ccaattggcg atcgatgcga aacggccacc ccaagggccc
     1141 cgggcgtcgg ctcggtgtcc cgcgggcgca aacgggatga gagccgggcc ggaagcggcc
     1201 gtgggaggct agcggggtga gacctgcccg ggactacgcc tacttcgacg cgccgccgct
     1261 ggcgttcgcg caccgtggcg gggcactcta cgagcccaac ctgcaccgtg agaacacgct
     1321 cgtcgccttc cgcaacgcgg tcgagctcgg ctaccgctac atcgagaccg acgtccacgc
     1381 gacgagcgac ggcgtcctgg tcgccttcca cgacaaggag ctcgaccggg tcaccgacgg
     1441 ccggggcgcc atctcggggc tgccctacgc gcaggtccgc gaggctcgca tcgccgggca
     1501 ggaaccgatc cccctgctcg ccgagatcct cgaggcgctc ccggccacgc gggtcaacat
     1561 cgacatcaag gcctcaggag ccatcggccc cctgtggcgt gcgatcgagg agcactccgc
     1621 acacgaccgg gtctgcgtcg cgtcgttctc caacctgcgc ctcgcccgct tccggcgcct
     1681 cgcgcgtggc cgggttgcca ccgcggccgg ctgggccggc acggccgccc tgcgcttcgg
     1741 tccagccgca ctgaccgccg tggtcagctc gcctggtcag gtcctgcaga tacccgaccg
     1801 gatgccactg gccggtcggc cggtcgaggt cgtgacgccc aggctgatcg agacggcaca
     1861 tcgggtcggc aagcaggtgc acgtgtggac gatcgacgac cccggggaca tgacccggct
     1921 gctcgacatg ggggccgacg gcatcgtctc cgaccgcatc gaggtgctcc gccgcgtcct
     1981 ggaagcccgc ggccagtggg cctgagcccg tccgagacgg atcgattccc acccgaggga
     2041 cccttcgcgc cggcctaccg gctcatctcg ctcgcgctcg tcgccctggt gagcatcgtc
     2101 gcgttcgagt tcatggcgat cacgacggcc atgcccgcag cggcgcagga gctcggcgcc
     2161 gtccggtcct acggcctggc gttctcggtc atgatcaccc tccaggtgct cggcatcgtg
     2221 ctcgccggag cgtggtccga cctgcgcgga ccgatgccgg cgatgtatgc cgggcagctg
     2281 ctcttcgccg ccggagcgac ggtctgcgca ctggccgcga gcttcccggt cttcttgctc
     2341 ggccgggccg tgaccggtct cggggccggg ctcatcgtcg tcgccgagta cgtcatcatc
     2401 ggccgggtct accccccggc gctgcgaccg cggttcttca ccatcgtctc cgcgggctgg
     2461 gtgctgccct cgctcgtcgg cccgccgatc tcggcgtggc tcacgacgtc gttctcgtgg
     2521 cgctgggtct tcgggatcgt cgcggtccca gccctggtca cgtttacggt catcgcgagc
     2581 caggccggac gggtcagggc gggcccgacg cctcccgagc agcccgacca gggcgagcca
     2641 ccaggtca
//
LOCUS       DATAIX010000745         1828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_145398, whole genome shotgun sequence.
ACCESSION   DATAIX010000745 DATAIX010000000
VERSION     DATAIX010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145398"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..>1828
                     /locus_tag="VHM65_11010"
     CDS             261..>1828
                     /locus_tag="VHM65_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX2706279.1"
                     /translation="MRGLRAPWVEGIVFLSVPELDVGGLTATGRANVFGRPNHAGLPD
                     LLEHLRRGGPVDAAAGRRLAQAVEQASIRQSPRARKVGTLQLRMPALQEGLGWQDYWA
                     RHERFLDDPPRRVRIYLVGTATTADQRGRLVRAAEREYRALRGIEFPGISAPIDFVEH
                     DLGPALVFPYDEGFVRLDHFLTEHGTRMSVDERLALVRSIAETMDYAHRRVLTHRSLG
                     PSCIWVRRDGEECAVRVTDWQTASRGSDTGTGVTGTRTIADLADAGASGYCAPEWAWA
                     TRSGVALDVFSLGATAYFVLTGQAPAASYAALTQRLHMQGALHLRQQLDGVPEALDQL
                     IADATAADPAKRTADVSAFLATLDEIRVTLKTPVEEALSPADPLQAEPGDEIDGFTVV
                     RVLGRGSTARALLVHTTSPDDISVLKVSLDPSKDGRLRGEADALAVLRDQPGIVRLED
                     GPLTIGNRLAILLSYAGERTLAQELRERGRLQPEWLHDWGRDLLDVVDALERRGVAHR
                     DLKPDNLGVHERGVG"
BASE COUNT          271 a          681 c          632 g          244 t
ORIGIN      
        1 acaccatcct gccgagcggc gagcctttcg cgcctgggcg cacggcgagt ttgtcgccgg
       61 cgacggttcg gtcaacgagg tcgacctcgt cctgatcacg ccaccggcgt cagcctggtc
      121 gagatcaagt cctggcgccg ccacccctcc ggcgacgtac gacttggcaa cagcaccacc
      181 gcgctccagt cgacaacccg gtcggactgg tcaacaagaa ggcccgcaag ttcaagtcgc
      241 tactgcaggc gacgaccgca atgcgcgggc tgcgggcgcc ctgggtcgag ggcatcgtct
      301 tcctgagcgt cccggagctc gacgtcggcg ggctgaccgc caccgggcga gcgaacgtct
      361 tcggccgacc gaatcacgcc gggctgccgg acctgctgga gcacctgcgg cgcggcggtc
      421 cggtcgacgc cgccgccggc cgccggctcg cccaggccgt cgagcaggcc agcatccggc
      481 agtccccgcg cgcccgcaag gtcggcacgc tgcaactcag aatgccggcg ctccaggagg
      541 ggctgggctg gcaggactac tgggcgcgac acgagcgatt ccttgacgac ccgccgcgcc
      601 gggtgcggat ctacctggtc ggcacggcga cgacggccga ccagcgcggc aggctggtcc
      661 gagccgccga acgggagtac cgcgccctgc gcggcatcga atttcccggc atctcggccc
      721 cgatcgactt cgtcgagcac gatctcggcc cggccctggt cttcccctac gacgagggct
      781 tcgtccggct ggaccacttc ctcaccgagc acggcacccg gatgtcggtc gacgagcggc
      841 tcgcgctggt ccgctcgatt gcggaaacca tggactacgc gcatcgccgg gtgctcaccc
      901 accgcagcct cggtccgagc tgcatctggg tacgccgcga cggcgaggaa tgcgccgtcc
      961 gggtcaccga ctggcagacc gcctcgcgcg ggtcggacac cggcaccggc gtgaccggta
     1021 cccgcacgat cgccgatctg gccgacgcgg gcgccagcgg ttactgcgcg ccggagtggg
     1081 cctgggccac ccgcagtggt gttgcgctcg atgtcttcag ccttggcgcg acggcgtact
     1141 tcgtcctcac cggccaggca ccggccgcct cctacgccgc cctcacccag cggctgcaca
     1201 tgcagggcgc gctgcaccta cgccaacagc tcgacggcgt gcccgaggca ctcgaccagc
     1261 tgatcgccga tgccaccgcc gccgacccgg cgaagcgcac cgctgacgtg tcggcgttcc
     1321 tggccacgct ggatgagatc cgggtcaccc tgaagacgcc ggtcgaggag gcgctgtcac
     1381 ccgccgaccc cctgcaggcc gagccgggcg acgagatcga cgggttcacc gtcgtccggg
     1441 tcctggggcg cggctcgacc gcccgcgcac tgctggtgca caccacgagc ccggacgaca
     1501 tcagcgtgct caaggtatcg ctggacccga gcaaagacgg ccgccttcgt ggggaggccg
     1561 acgccctggc cgtgctgcgg gaccagccgg ggatcgtccg gctcgaggac ggtccgctca
     1621 ccatcggcaa ccggctggcc atcctgctgt cgtacgccgg ggagcgcacg ctcgcccagg
     1681 agctgcgcga gcgaggccgg ctgcaaccgg agtggctgca cgactggggc cgcgatctgc
     1741 tcgacgtcgt cgatgcgctg gagcgccgcg gcgtggcgca ccgcgaccta aagccggaca
     1801 acctgggggt gcacgagcgc ggcgtagg
//
LOCUS       DATAIX010000746         2201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_265393, whole genome shotgun sequence.
ACCESSION   DATAIX010000746 DATAIX010000000
VERSION     DATAIX010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265393"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VHM65_11015"
     CDS             <1..485
                     /locus_tag="VHM65_11015"
                     /inference="COORDINATES: protein motif:HMM:NF036831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX2706280.1"
                     /translation="PLPEAQLDRFLLKVSLPVPPRDQEIDIVARHARGFDPRDLAAAG
                     IRPVASAADLAAGSAAVRRVSVAADVAAYIVDIARATRVSPSLSLGVSPRGATALLAT
                     SRAWAWMIGRDFVTPDDVKALAQATLAHRLTLRPEAELEGVSTASVLAAAISSVPVPR
                     "
     gene            569..1861
                     /locus_tag="VHM65_11020"
     CDS             569..1861
                     /locus_tag="VHM65_11020"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX2706281.1"
                     /translation="MAVSWRFPVLVLGGLVAVFLAPAASTVWLWLGVALLVTGIDIWL
                     AISPKALGLAREPVGSLRLGATGRSSLLLTNPATRTLRGVVRDAWQPSAGAVGDRHTV
                     VLRPGERRRLTTSLTPTRRGDRLADRVTVRSFGPLRIGARQRSIPVSGSIRALPPFGS
                     RKHLPSRLARLRQLDGRSAVRVRGAGTEFDSLRDYVEGDDVRSIDWRATARRQTVVVR
                     TWQPERDRRVILVLDTSRTSAGRVLDIPRLDAAMDAALLLAALASRAGDRVDLIAGDR
                     RVRAEVTGRPRTTLLADLVQAMAPLDAALVEANWSVLAGRVSTLSRRRALVVLLTPLE
                     PAAIEESLLPTVAALTRHHRVVLASVADPAVETMAATRSSVAEAYDAAAAERTRALRE
                     RTAAALGRLGVTVVDAGPEELPPALADHYLLLKSQGLL"
     gene            2011..>2201
                     /locus_tag="VHM65_11025"
     CDS             2011..>2201
                     /locus_tag="VHM65_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706282.1"
                     /translation="MAPIEAGLVEVVTSTFNIWSRTAPMTRTLLTVPTPGRYRKGSHS
                     TSTRAPTMTDHVPMESPSR"
BASE COUNT          278 a          868 c          748 g          307 t
ORIGIN      
        1 acccactgcc cgaggcccag ctcgaccggt tcctgctcaa ggtgagcctg ccggtgcctc
       61 ctcgcgacca ggagatcgac atcgtcgccc gccacgcccg cggcttcgat ccccgcgacc
      121 tcgcggccgc ggggatccgg cccgtggcgt ctgcggccga tctggctgcc gggtcggctg
      181 ccgtccgccg ggtgtcggtc gccgccgacg tcgccgccta catcgtcgac atcgcccggg
      241 ccacccgcgt ctcgccgtcg ctctcgctcg gggtgagccc gcgtggagcc accgcgctgc
      301 tcgcgacgag ccgcgcctgg gcctggatga tcggccgcga cttcgtcact cctgacgacg
      361 tcaaggccct ggcccaagcc accctcgccc accggctgac gctgcgcccc gaggccgagc
      421 tcgagggcgt cagcaccgcg tcggtgctcg ccgccgccat ctcctccgtg ccggttccgc
      481 ggtagcagca cgcgggccgg gactgaccag gccaagagac gaaggaccca aggaccaagg
      541 accaaggaat aagggcagag gatcaggtat ggcggtcagc tggcggttcc cggtgctcgt
      601 cctcggtggc ctcgtcgcgg tgttcctcgc cccggccgca tcgacggtgt ggctctggct
      661 cggggtggcc ctgctcgtca ccggcatcga catctggctc gccatctcgc ccaaggccct
      721 cggtctggcc cgcgaaccgg tggggtcgct tcggctcggg gcgaccggcc ggagcagcct
      781 gctgctgacc aacccggcca cgcgcacctt gcgcggggtc gtccgcgacg cgtggcagcc
      841 gtccgcgggg gccgtgggag accgccatac cgtcgtgctc cgtcccggcg agcggcgacg
      901 cctgaccacc tcactcaccc cgacgcggcg gggggaccgg ctcgccgacc gggtcacggt
      961 acgcagcttc ggaccgctgc ggatcggcgc gcggcagcgc tcgatccccg tctccgggtc
     1021 gatccgggcc ctgccgccct tcggctcgcg caagcacctg ccgtcgcgac tggcccggct
     1081 gcgccagctc gacggccgtt ccgcggtccg ggtccgaggt gcggggacgg agttcgactc
     1141 gctgcgcgac tacgtcgagg gcgacgacgt ccgcagcatc gactggcggg ccaccgcgcg
     1201 acggcagacc gtcgtcgtcc gcacgtggca gcccgaacgg gaccggcgcg tcatcctcgt
     1261 cctcgacacc tcgcgcacct cggccgggcg ggtcctcgac atcccccggc tcgacgcggc
     1321 catggacgcg gcgctgctgc tcgccgccct cgcctcccgc gccggtgacc gggtcgacct
     1381 cattgccggc gaccgccggg tccgggccga ggtgacgggc aggccgcgca cgacactgct
     1441 cgccgacctc gtgcaggcca tggcccccct cgacgccgcc ctcgtcgagg ccaactggag
     1501 cgtcctcgcc gggcgagtca gcacgctgag ccgtcgccgt gcgctcgtcg tgctgctgac
     1561 gcccctcgaa cccgccgcca tcgaggagtc gctcttgccg acagttgctg ccctcacccg
     1621 tcaccaccgc gtcgtgctcg catcggtcgc cgacccggcg gtcgagacga tggcggcgac
     1681 ccgctcgtcg gtcgcagagg cgtatgacgc cgcagccgcc gagcgcaccc gcgccctgcg
     1741 cgagcggacc gccgcggccc tcggacggct cggcgtcacc gtggtcgatg ccggtcccga
     1801 ggagctgccc cccgccctgg ccgaccacta cctgctgctc aagagccagg gcctgctcta
     1861 gctggtcctt cgggcgcact caccacgact gcgcagacca tacgtgcacc tgccagaccg
     1921 cgcgtcccat agtggcaccg cgcgccgcat atttcgtggg cgcggtgcca ctatggggcg
     1981 cgaggtcctg gcgttcggcc ggacctggca atggcgccca tcgaggccgg gctggtcgag
     2041 gtcgtgacgt cgaccttcaa catctggtcg agaaccgccc cgatgacaag gacgttgctc
     2101 accgtgccca cccccggtcg ttaccgcaag ggcagccaca gcacgagcac gagggcgccg
     2161 acgatgaccg accacgtgcc gatggagagc cccagccgct g
//
LOCUS       DATAIX010000747         1732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_145614, whole genome shotgun sequence.
ACCESSION   DATAIX010000747 DATAIX010000000
VERSION     DATAIX010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145614"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VHM65_11030"
     CDS             <1..235
                     /locus_tag="VHM65_11030"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706283.1"
                     /translation="DYLRVFLSGASDEIPDKQGRVTIPSTLRAYAGLARDCAVIGAGS
                     HVEVWDGAAWATYLAATEQGFADQSQEVMPGFF"
     gene            422..1402
                     /gene="rsmH"
                     /locus_tag="VHM65_11035"
     CDS             422..1402
                     /gene="rsmH"
                     /locus_tag="VHM65_11035"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX2706284.1"
                     /translation="MSAPGAAAAPRHIPVMRERVVELLGPALSRPGAVYVDATLGMGG
                     HAEAVLDGFEHVRVVGIDRDRQALELAGKRLARFPGRVELVHAVYDEFAEVADRLGLE
                     TIDAALFDLGVSSLQLDESRRGFAYRVDAPLDMRMDQESGRTAADVVNGYSADDLERV
                     IREYGEERFARRIARAMVRTRATEPFTRTGQLALLLKAVVPAASQRSGGHPAKRTFQA
                     LRIEVNAELDAWRDALPAAIGRLGVGGRVVVLSYHSLEDRLTKTTLAAGATSSTPAGL
                     PVELPEHAPYLALLTRGAEEPSAAEVAANPRAASARLRAAERIRSTRRSR"
     gene            1399..>1732
                     /locus_tag="VHM65_11040"
     CDS             1399..>1732
                     /locus_tag="VHM65_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706285.1"
                     /translation="MSQPMATARAFPRSWPEGAPRRALAPLRVVPAAIRRQGHGGFAL
                     LCIGLLTTGLLGLLLLNTTLGQGAFTMQDLRVRSTGLAGQEEALTQAIDQNRSPAALA
                     SRASAMGMV"
BASE COUNT          226 a          655 c          622 g          229 t
ORIGIN      
        1 cgactacctg cgcgtcttcc tctccggcgc ctcggacgag atccccgaca agcaagggcg
       61 ggtgacgatc ccctcgacgc tgcgcgccta tgccgggctg gcgcgcgact gcgcggtcat
      121 cggtgcgggg tcacacgtcg aagtgtggga cggcgcggcc tgggccacct acctcgcagc
      181 caccgagcaa gggttcgccg accagtcgca ggaggtgatg cccggcttct tctagaccag
      241 cgcgcagcac ctggggccag gcctccagcc gcccgccttc ccggcgcgac ttcccccgcg
      301 ccgggcggtg acggcggatg gggacctgga cgcaggttcg gcccggccgg tcaccctccg
      361 gaacagcaca gacccgcaca cagcacggac ccgcacactg cacacgaagg acgacatgac
      421 gatgagcgca ccaggggcgg ccgcagcgcc gcggcacatc ccggtcatgc gtgagcgcgt
      481 cgtcgagctg ctcgggccgg ccctgtcccg gccgggggcc gtgtatgtcg acgccaccct
      541 cggtatgggc gggcacgccg aggccgtgct cgacgggttc gaacacgtcc gggtcgtcgg
      601 gatcgaccgc gaccggcagg ccctggagct cgcggggaag cgcctggcgc ggttccctgg
      661 ccgggtcgag ctcgtccacg cggtgtatga cgagttcgcc gaggtcgccg accggctcgg
      721 gctcgagacc atcgacgcgg cgctgttcga cctcggggtg tcctcgctcc agctcgacga
      781 gagccggcgt ggcttcgcct accgggtcga cgccccgctc gacatgcgca tggaccagga
      841 gtccggccgc acggcggccg acgtcgtcaa cggctactcc gccgacgacc tcgagcgggt
      901 catccgcgag tatggcgagg agcgcttcgc ccgccggatc gcccgggcca tggtgcgcac
      961 ccgcgcgacg gagccgttca cccgcaccgg gcagctcgcc ctgctgctca aggcggtcgt
     1021 cccggcggcg tcccagcgca gcggcgggca cccggccaag cggaccttcc aggccctgcg
     1081 gatcgaggtc aatgccgagc tcgacgcctg gcgcgacgcc ctgccggcgg cgatcgggcg
     1141 cctcggtgtc gggggccggg ttgtcgtcct gagctatcac tcgctcgagg accggctcac
     1201 caagacgacg ctggccgccg gggccacgag ctcgaccccc gcaggcttgc cggtcgagct
     1261 tcccgagcac gcgccatacc ttgccctgct cacccgcggc gccgaggagc cgagtgcggc
     1321 cgaggtcgcg gccaacccgc gggccgcgtc ggcccggctc cgcgccgcag agcgcatccg
     1381 ttccacgagg aggtcccgat gagccagccg atggccactg cccgtgcgtt cccgcgctcc
     1441 tggcccgagg gcgccccgcg gcgggcgctg gcacccctgc gggtcgtccc cgccgcgatc
     1501 cggcgtcagg ggcacggcgg cttcgccttg ctgtgcatcg ggctgctgac gacgggcctg
     1561 cttgggctgc tcctgctcaa cacgacactg ggccaggggg cgtttacgat gcaggacctg
     1621 cgggtccgct cgaccggcct cgccgggcag gaggaggcgc tgacccaggc gatcgaccag
     1681 aaccgcagcc ctgctgcgct cgcctcgcgc gcctccgcga tggggatggt gc
//
LOCUS       DATAIX010000748         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_145615, whole genome shotgun sequence.
ACCESSION   DATAIX010000748 DATAIX010000000
VERSION     DATAIX010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145615"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VHM65_11045"
     CDS             complement(<1..550)
                     /locus_tag="VHM65_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2706286.1"
                     /translation="MSVTEGRNARALPAQASPPPPASVRTLLDAVVAVGSDLHLSEVL
                     ARVVRSACDLVDAEYGALGVLRPGGDDVVEFIVEGISSQERVVIGELPRGAGILGLLV
                     RDPRPLRLADLTKHPSFRGFPPGHPPMRTFLGAPVRVRGVVFGNLYLTEKRGGGPFTD
                     NDEAIVTTLAAATGVAIDNARIY"
     gene            complement(537..1199)
                     /locus_tag="VHM65_11050"
     CDS             complement(537..1199)
                     /locus_tag="VHM65_11050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2706287.1"
                     /translation="MTISVYLLDDHEIVRRGLRELLERSGGIRVIGESGTAREAVRRI
                     PALRPDVAVLDARLPDGSGIDVCRQIRGVDSSIKGLILTSYDDEEALRASVLAGASGY
                     VLKDVRGNDLVSAIRRVAAGEHLIEAAVVERMKTSLAEDALDPRLRALTAQERRILDR
                     VAQGMTNRQIALDMSLAEKTVKNYVTSILDKLGMERRTQAAVFAVSQRNDGRSGRRGE
                     RY"
     gene            complement(1251..>1555)
                     /locus_tag="VHM65_11055"
     CDS             complement(1251..>1555)
                     /locus_tag="VHM65_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2706288.1"
                     /translation="VFVQDYWVATGDPKIVEAHRIACREAVDEARALVRAEHPELSVT
                     TLISEGDPAQTLAEAGREAGLVVVGSRGRGGFSGLLLGSVSHRVIHTATCPVAVVR"
BASE COUNT          229 a          548 c          565 g          213 t
ORIGIN      
        1 cgtagatccg cgcgttgtcg atggcgaccc cggtggctgc ggccagggtg gtaacgatcg
       61 cctcgtcgtt atcggtgaag gggccgcctc cccgcttctc ggtgaggtag aggttgccga
      121 agacaacgcc gcggacccgg accggggccc cgaggaaggt ccgcatcggc gggtggccgg
      181 gcgggaaacc ccggaacgag gggtgcttgg tcaggtccgc gaggcgcagc ggacgggggt
      241 cgcgcacgag caacccgaga atgccggcgc ccctgggcaa ctcgccgatg acgacccgct
      301 cctgggagct gatcccctcg acgatgaact cgaccacgtc gtccccaccc ggtcgcagca
      361 cgccgagcgc cccgtactcg gcgtcgacga ggtcgcacgc gctgcggacg acccgagcga
      421 gcacctccga caggtgcagg tcgctcccga ccgcgacgac ggcgtccagc agggtccgca
      481 ccgacgccgg cggtggcggc gacgcctggg caggcaaggc tcgggcgttg cgcccttcag
      541 taacgctcac cgcgtctccc gctccgcccg tcgttgcgct gggacaccgc gaagaccgct
      601 gcctgggtgc gtcgttccat cccgagcttg tccaggatcg aggtgacgta gttcttcacg
      661 gtcttctcgg ccagggacat gtccagggcg atctgccggt tcgtcatgcc ctgggcgacg
      721 cggtcgagga tgcgccgttc ctgggccgtg agcgcgcgca gtcgcgggtc gagggcgtcc
      781 tcggccagcg aggtcttcat ccgctcgacc accgcggcct cgatgaggtg ctccccagca
      841 gcgacgcggc ggatcgccga gacgaggtcg ttgccgcgga cgtccttgag gacatacccc
      901 gacgccccgg cgagcacgga cgcgcgcagc gcctcctcgt cgtcgtagga ggtgaggatg
      961 aggcccttga tcgagctgtc gacgccgcgg atctgccggc acacgtcgat gccggagccg
     1021 tcgggcaggc gtgcgtcgag caccgccacg tcggggcgca gggcggggat gcggcgcacc
     1081 gcctcccggg ccgtgccgga ctcgccgatg accctgatcc cccccgatcg ctcgaggagc
     1141 tcgcgaagcc cgcgacggac gatctcgtgg tcgtccagga ggtacacgct gatggtcacg
     1201 gggcaagcga atcacaccgg ctggtgaatg acctaccccg ggcggctggg tcagcgcacg
     1261 acagccacgg ggcaggtcgc cgtatggatg acccggtggc tcacggaccc gagcaggagg
     1321 ccgctgaagc caccacggcc gcgactcccg acgacgacga gacccgcctc ccggcccgcc
     1381 tcggcaaggg tctgggcggg gtcgccctcc gagatcagcg tcgtgacgga cagctcgggg
     1441 tgctccgccc ggaccagggc ccgcgcctcg tcgacggcct cgcggcaggc gatccggtgg
     1501 gcctcgacga tcttggggtc gccggtggcg acccagtagt cctggacgaa cacgt
//
LOCUS       DATAIX010000749         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_145827, whole genome shotgun sequence.
ACCESSION   DATAIX010000749 DATAIX010000000
VERSION     DATAIX010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145827"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VHM65_11060"
     CDS             <1..784
                     /locus_tag="VHM65_11060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX2706289.1"
                     /translation="IVTVPLSYGGQPLGTLEVAPPPRGLTDAGRALLAALAPQVAVIS
                     HAAALNMQLEDARRHVIDAAQAERARLRRDLHDGLGPSLSGVALGLESVRGALLNNQD
                     RARQILDRAQAEVRAAVEEVRRILDDLRPAPLDAFGLVGAVRAHVLDSPDGLAVQVSA
                     DALAHLDPDVEAAAYRIAVEALTNVRRHAAADNCTVTLSTDDQQVHLEVHDDGRGLPA
                     EVSHGLGLASMRHRAESLGGDLDVRSGSDGTTVNARLPRQPG"
     gene            781..1437
                     /locus_tag="VHM65_11065"
     CDS             781..1437
                     /locus_tag="VHM65_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2706290.1"
                     /translation="MTPNPVTVVLADDHPMFRYGLRAVLDDAPEIEVVGEAASGRTLL
                     DVVASTHPDVVLTDLAMPDLDGAAATRQLLARDPNLGILILTMHDDDESVFAALRAGA
                     RGYLLKGAEGPELIRAVLAIAAGDAVYGSAVARRIVAFYSGARQNYTAQAFPELTPRE
                     RDVLDLLATGARNHEIARRLELSEKTVRNHISALLLKLQVPDRTTAAARARDAGLGHS
                     "
     gene            1855..2565
                     /locus_tag="VHM65_11070"
     CDS             1855..2565
                     /locus_tag="VHM65_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX2706291.1"
                     /translation="MNIRDRVGATATPPQASPNPRAVATPACQDKQAMKAVIRVLLGL
                     FALSVCLTLAAPTVSAQPAASSSPSQAVTAFIPVSNLPAWPVLGQGSNSDWPRSTVRS
                     LQYLLNARGAHLVVDGFFGPRTDAAVRSYQRTHGLVVDGIVGPKTWSSAIITVRRGSV
                     GPAVEAVQDQANSRGVNRAGIVLAVDGIFGRNTDGWVRAFQSAIAGHVIGFREDGVVG
                     PLTWSLLLTGEKPNPLYP"
BASE COUNT          397 a          955 c          817 g          397 t
ORIGIN      
        1 gattgtcacg gtcccgctca gttacggcgg gcagccgctg gggacgctcg aggtcgctcc
       61 gccaccccgc gggctgaccg acgccggacg ggcactgctg gccgcgctcg ccccccaggt
      121 cgcggtcatc tcccacgccg cagcgttgaa catgcaactc gaggatgccc gccggcacgt
      181 catcgatgct gcgcaggccg agcgggccag gctgcgccgt gatctgcatg acgggctcgg
      241 gccgtcgctg tcgggggtcg cgctcgggct ggagtccgtg cgcggcgcgc tgctgaacaa
      301 ccaggaccgg gcccggcaga tcctggaccg agcgcaggcc gaggtccgcg cagccgtgga
      361 agaggtgcgg cgcatcctcg acgatttgcg ccccgcgccg ctggatgcgt tcggcctggt
      421 cggcgccgta cgtgcccacg tgctggacag ccctgacggg ctcgccgtcc aggtcagtgc
      481 ggacgccctg gcccacctcg atcccgacgt ggaggccgcg gcgtatcgca tcgcggtcga
      541 ggcgctcacc aacgtccggc ggcacgcggc cgccgacaac tgcaccgtca ccctgtccac
      601 cgacgatcag caggtgcacc tagaggttca cgacgacggc cgcggcctgc ccgccgaggt
      661 cagccacggg ctcgggttgg cgtcgatgcg tcaccgcgcc gagtcactcg gcggtgacct
      721 cgacgtgcgc tcggggtccg acggcaccac cgtgaacgcc cggctgcctc ggcaacccgg
      781 atgaccccca acccggtcac cgtcgtactc gccgacgacc acccgatgtt ccggtacgga
      841 ctgcgcgccg tcctcgacga cgcaccggaa atcgaggtcg tcggggaggc agcatccggc
      901 cgcaccctgc tggacgtcgt cgcctcgacc caccccgacg tggtcctcac cgaccttgcc
      961 atgcccgacc tcgacggcgc cgccgccacc cgacagctgc tggcccgcga ccccaacctc
     1021 ggcatcctga tcctgaccat gcacgatgac gacgagtccg tgttcgccgc actgcgcgca
     1081 ggggcccggg ggtatctgct caagggagcc gagggccccg aactgatccg ggctgtgctc
     1141 gctatcgccg ccggcgacgc cgtctacggt tccgctgtcg cccgccgcat cgtcgccttc
     1201 tacagcgggg cgcgccaaaa ctacaccgcc caggccttcc ccgaattgac ccctcgggaa
     1261 cgcgacgtac tcgacctgct cgccaccggc gcccgcaacc acgaaatcgc ccgccgactc
     1321 gaactgtccg agaagaccgt ccggaaccat atttccgcgt tgctcctcaa actccaggta
     1381 cccgaccgca ccaccgccgc cgcacgagcc cgcgacgccg gcctcggaca cagctgacca
     1441 acccgacata ccgcaaagga atccgcctgc gggacgcgat ggctcgacct cattgctagc
     1501 gacgggagag cgcgcgccgt ctcgtttcgg cgggccaaac tcggacaatc cccggttgtg
     1561 cgagttggca ggtcccgggc caggccctct cgtttgtgcc gctcaacgct gagccgggca
     1621 gtcgccttcg aggtcagatg tctcgctcgc gccccggtcc gcgtccggcg ttcgggaagg
     1681 gtccgggact cccgatcacg cgtccgtccc ttcggtcatc cttcggtcat cgccgacctt
     1741 gtggccaccc gggtaacgat ccgacggcga aacgcactcc gttaggacac gggccacaac
     1801 cctctcgggc gactcgccgt catcgctaca ccggacaacc cgaggaggac gaccatgaac
     1861 attcgcgatc gggtcggagc gacagcgacg ccgccgcagg cctcgcccaa cccgcgggcc
     1921 gttgcgacgc ccgcttgcca agacaagcag gcgatgaagg ccgtaattcg cgtgctgttg
     1981 ggcttgttcg ccctcagcgt ttgcctcacc ctcgcggccc cgaccgtttc agcccaacca
     2041 gcagcttcat cgagcccatc ccaggcggtc acggcgttca tcccagtgtc caacctgccg
     2101 gcctggccag tgctgggtca gggcagcaac agcgactggc cgcggtcgac cgttcgctcg
     2161 ttgcagtacc tgctcaacgc ccgcggcgcc catcttgttg tggacggctt cttcggtcca
     2221 aggaccgatg ctgccgtgcg gtcgtatcaa cggacccacg ggcttgtggt cgacgggatc
     2281 gtcggaccca agacctggtc gtcggcgatc atcaccgtcc gtcggggaag cgttgggccc
     2341 gcggtggagg ccgtgcagga tcaggccaat tcccgaggcg tgaacagggc aggcattgtt
     2401 ctggccgttg acggcatctt cggacgaaat accgacgggt gggtgcgcgc cttccaatcc
     2461 gcgattgcgg gtcacgtcat cggcttccga gaggacggcg tcgtcgggcc gctgacctgg
     2521 tcgttgctcc tcacgggtga gaaacccaat ccgctgtacc cgtaac
//
LOCUS       DATAIX010000750         2328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_145839, whole genome shotgun sequence.
ACCESSION   DATAIX010000750 DATAIX010000000
VERSION     DATAIX010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145839"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VHM65_11075"
     CDS             <1..205
                     /locus_tag="VHM65_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706292.1"
                     /translation="AAAYAAKAAGLAAPDDPTAVAAEVRWQHGRASPAVRDVLRRLPP
                     PPRAAGTLGSLVSDIHTTLADGA"
     gene            202..453
                     /locus_tag="VHM65_11080"
     CDS             202..453
                     /locus_tag="VHM65_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706293.1"
                     /translation="MTACASRDATLEEHTRGSRAGLVTSQDEYVGTCLGRSHGLVDTY
                     GLALLEPHGPAGSRGAFRAVLDSDDDEWDRGKTLAVRAD"
     gene            688..>2328
                     /locus_tag="VHM65_11085"
     CDS             688..>2328
                     /locus_tag="VHM65_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2706294.1"
                     /translation="MTKPLTDVDLTRREMLAMAVVGGAGLAISGASSAEAAGATSSAA
                     SATGAKRRFLLTVLGTTDLHGHVYNWDYYTNAEFDNSAHDDIGVAKVATLIKAVRAER
                     GARSCLTIDAGDTIQGTPLSYYYAVIDPITGGSTHPMAAAMNAVRYDAAALGNHEFNY
                     GMDTLRAFERQCRFPLLAANAVGWRSGRPVFRPYVIRRVHVPGHEPVRVGILGLVTPG
                     VAIWDKANVEGRVRFPGIVEQARVYVPRLRAAGADVVIVSAHSGADTSSSYGDALPYP
                     ENASTLLAEQVRGIDAILVGHAHKEIAQRFVTNTATGRQVLLSEPYYWGARVTVMDLA
                     LERTRGRWRVVQSSATLLNSNAVPEDPAIAALVRPAHERVLTYVNSPIGSCLQAMSAA
                     TSRYEDTAAMDFINVVQAGAVKAALAGTPEAALPVLSIAAPFNKAAAIPQGEVTVRDV
                     AGLYIYDNTLLGIVLTGEQIRAYLEKSAEYFKQVTGPGPFAADDVTNAVTPSAPGGTP
                     DYNYDILGGLDAALAYDIDIAAAPGGRITNLLYGGSPVA"
BASE COUNT          358 a          890 c          776 g          304 t
ORIGIN      
        1 agcggcagcc tacgccgcga aggccgctgg gctggccgcg cccgatgatc cgaccgccgt
       61 cgccgccgag gtccggtggc agcacggccg ggcctcgccg gcggttcggg atgtcctgcg
      121 gcggctgccg cccccgcccc gtgctgcggg cacactcggg tcgctggtca gcgacataca
      181 cacgacgctg gcagacggcg catgacggct tgcgcgagcc gcgatgccac gctggaggag
      241 cacacgagag gctcgcgggc ggggctggtg accagccagg acgagtatgt cggcacctgc
      301 ctcggccgta gccacggact ggtcgacacc tacggactcg ccctcctgga acctcatggc
      361 cccgccggca gtcgcggcgc attccgggcc gtcctcgact cggacgacga cgaatgggac
      421 cgcggcaaga cgctcgctgt gcgcgctgac tgaatgacgc cgcgctcgac cggctcggtc
      481 acagcaccta cccgaacggc agtgacgccg aggagccgtt catcgatgtc gtctggtggg
      541 tgaaccagga ggtactccac cgcggggccg aagaggggca agggcacccc cgcccgggtg
      601 gctggccccg aaccaccgcg gtagcgtcgc aagcggccgg gtccacccgg gaggccggct
      661 ctgatcgtgc gaccaaggtg cgtgcccatg acgaagcccc tgaccgacgt cgacctgacc
      721 cggcgagaga tgctcgccat ggccgtcgtg ggaggtgccg ggctcgccat ctcgggggcc
      781 tccagcgccg aggccgccgg cgccacgtcc tcggcagcct cggcgaccgg ggccaagcgc
      841 cggttcctgc tcaccgtcct gggcaccacg gacctgcacg ggcacgtcta caactgggac
      901 tactacacga acgccgagtt cgacaactcg gcccacgacg acatcggcgt cgccaaggtg
      961 gccaccctca tcaaggccgt ccgggccgag cgaggcgccc gctcgtgcct gaccatcgac
     1021 gccggcgaca cgatccaggg cacgcccctg tcctactact acgccgtcat cgacccgatc
     1081 accggcgggt ccacccaccc gatggccgca gccatgaacg ccgtccggta tgacgccgcc
     1141 gccctgggca accacgagtt caactacggc atggacaccc tgcgcgcgtt cgagcgccag
     1201 tgccgcttcc ccctgctggc cgccaacgcg gtcggctgga ggagcgggcg ccccgtcttc
     1261 cgtccctacg tcatcaggag ggtccacgtc cccgggcacg aaccggtgcg ggtcggcata
     1321 ctcggcctcg tcactcccgg agtcgccatc tgggacaagg ccaacgtcga gggccgggtg
     1381 cgcttccccg gcatcgtcga gcaggcccgc gtctacgtcc cgcggctgcg ggccgccggg
     1441 gccgacgtcg tcatcgtctc ggcgcactcc ggtgccgaca cctcctcgtc atacggggac
     1501 gccctgccct accccgagaa cgcgagcacc ctactcgccg agcaggttcg gggcatcgac
     1561 gcgatcctcg tcgggcacgc ccacaaggag atcgcccagc gcttcgtgac gaacaccgcg
     1621 accgggcgtc aggtgctgct gtccgagccg tactactggg gggcgcgggt cacggtgatg
     1681 gacctggctc tggagcggac ccgcggccgg tggcgggtcg tgcagtccag cgccaccctg
     1741 ctcaactcca acgccgtgcc cgaagacccc gcgatcgcgg cgctggtccg ccccgcgcac
     1801 gagcgggtcc tcacctacgt caactccccc atcggcagct gcctgcaggc catgtccgcg
     1861 gcgacctcac gctacgagga caccgcggcg atggacttca tcaacgtcgt ccaggccggc
     1921 gcggtcaagg ccgccctcgc cggcaccccc gaggcagccc tgcccgtcct gtcgatcgcc
     1981 gcgccgttca acaaggcggc ggccatcccg cagggcgagg tcaccgtccg cgacgtggcc
     2041 gggctctaca tctacgacaa cacgctgctg ggcatcgtgc tgaccgggga gcagatcagg
     2101 gcctacctgg agaagtcggc ggagtacttc aagcaggtca ccggcccggg cccgttcgcc
     2161 gccgacgacg tcaccaacgc cgtgacgccc agcgcccccg gcggcacgcc ggactacaac
     2221 tacgacatcc tcggcgggct cgacgcagcg ctggcctacg acatcgacat cgccgcggcc
     2281 ccgggcggcc ggatcaccaa cctgctgtat ggcgggtcgc cggtcgcc
//
LOCUS       DATAIX010000751         2370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_145857, whole genome shotgun sequence.
ACCESSION   DATAIX010000751 DATAIX010000000
VERSION     DATAIX010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145857"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1824
                     /locus_tag="VHM65_11090"
     CDS             <1..1824
                     /locus_tag="VHM65_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706295.1"
                     /translation="GGSRVFEAPGVDPVENHAPDQPAYLIHGGTQLTREEAATLETAM
                     NHHESACRLSVMYSYGYTVEVPQAPAPADPPPHLHVIDERIRVRPRFGDGTKVRPMVQ
                     FGAIGEAVTAASATAATSSAAVVSAASTTRMDAFTVRKRVSPVLRDMVISGHLIDLIT
                     AQATRPESRQQTVTRTVPFVFEPSDEQNGPIYRSLHGAANLTEAWERGEAGWMCESVF
                     PNTVNRLPDAMRLAWNPELGGPHMIPTLHRDSTGTPRVRLLMRLAPFHDPRQRTLVRR
                     IVGMPAATVIIGEVEGSTLRLGGSFPEELAVIGDTGAPAPLTGVDLTLDLSLAYYQLF
                     CQQISTPVGVPGQVSVVLDTPPATEGQAPVPQTTQVEVSLRLDRVDDLPCALAVPDSP
                     SPATVTVTNQSGADLTIGGVAVTLLQTDQDSAVPVDTFPGRCTTSFPVSLAAGASTDL
                     AIEPDAGPGDETAAGFLWNAVLVELLDKRMVAAPDQMLKHVHELAGGSEISRDITVSS
                     PVFSTGSLPAKWASLASIEVEVTPPDGTPVSVVLSLTGASRTVTASVSLHAVATGASG
                     GITTVGYRVRNNYLDHQGAWGATQQSSGSELIAYPNPAEGD"
     gene            1826..>2370
                     /locus_tag="VHM65_11095"
     CDS             1826..>2370
                     /locus_tag="VHM65_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706296.1"
                     /translation="MDDAASLAGSAVRHLSALAHVSGGWTDFWVGAGTSTSWVTGYAG
                     HALGRAAYHRGLPRELRALAHASAASAVGALERQQRHGAWGWSPAVRADADSTAWCVR
                     ALVSMGRPVGSRTWDFLAGHRSADGYRTYRPTEQTGRWAQPMPEVTAVVLLAYLEADR
                     ITPAELGERWLQWCGPTGPWT"
BASE COUNT          331 a          911 c          811 g          317 t
ORIGIN      
        1 ggcggcagcc gggtcttcga ggcacctggg gtcgaccccg tcgagaacca cgcgccggac
       61 cagccggcct acctgatcca cgggggcacc cagctcaccc gggaggaggc cgcgaccctg
      121 gagacggcga tgaaccacca cgagtccgcc tgccggctca gcgtgatgta ctcgtacggc
      181 tacacggtcg aggtgccgca ggcgccggct cccgccgacc cgccgcccca cctgcacgtc
      241 atcgacgaac ggatccgcgt gcgcccgagg ttcggggatg ggaccaaggt tcgccccatg
      301 gtgcagttcg gggccatcgg cgaggccgtc acggccgcct ccgcgacggc ggcgacctca
      361 tcggccgcgg tggtctcggc cgcgagcacg acccggatgg atgccttcac ggtgcgcaag
      421 cgggtctcgc ccgtactgcg cgacatggtc atctcggggc acctcatcga cctcatcacg
      481 gcccaggcga ccaggcccga gtcccgccag cagacagtga cccggaccgt gccgttcgtc
      541 ttcgagccct cggacgaaca gaacgggccg atctaccggt cgctgcacgg cgcagccaac
      601 ctcaccgagg cctgggagcg cggtgaggcg ggctggatgt gcgagtcggt cttcccgaac
      661 accgtgaacc ggctccccga cgcgatgcgc ctggcctgga accccgagct gggcgggccg
      721 cacatgatcc cgacgctgca ccgggactcg accggcacgc cccgcgtccg cctgctcatg
      781 cgcctggccc ccttccacga cccgcgccag cgcaccctcg tgcgcaggat cgtcgggatg
      841 ccggccgcca ccgtcatcat cggcgaggtc gaggggagca ccctgcgcct cggcggctcg
      901 ttccccgagg agctggccgt catcggcgac accggcgcac cggcccccct gaccggcgtc
      961 gacctcaccc tcgacctgtc cctcgcctac taccagctgt tctgccagca gatctcgacg
     1021 ccggtcggcg tgccgggcca ggtctcggtc gtcctcgaca ccccacccgc gaccgagggt
     1081 caggcgccgg tccctcagac gacccaggtc gaggtgtcgc tgcgcctgga ccgggtcgac
     1141 gacctcccgt gcgcgctcgc cgtcccggac agcccgtcgc cggccaccgt gacggtgacc
     1201 aaccagtcgg gtgccgacct caccatcggt ggcgtcgcgg tcacgctgct ccagaccgac
     1261 caggactcgg cggtgcccgt cgacaccttc ccgggccggt gcacgacctc gttcccggtc
     1321 tccctcgcgg ccggagcgag caccgacctc gcgatcgaac cggacgccgg tcctggcgac
     1381 gagaccgcgg cgggcttcct ctggaacgcc gtcctcgtcg agctcctcga caagcggatg
     1441 gttgcggccc cggaccagat gctcaagcac gtacacgagc tcgctggggg gtccgagatc
     1501 agccgcgaca tcacggtgag ctcaccggtc ttcagcaccg gctccctccc cgccaagtgg
     1561 gccagcctgg ccagcatcga ggtcgaggtc accccacccg acgggacgcc ggtctcggtc
     1621 gtcctgagcc tcaccggcgc cagcaggacg gtgacggcct cggtgtcgct gcatgccgtg
     1681 gccacgggag cctccggcgg gatcaccacg gtcggctacc gggtgcgcaa caactacctc
     1741 gaccatcaag gcgcctgggg cgccacgcag cagagctccg gcagcgagct catcgcctac
     1801 ccgaaccccg cggagggtga ctgacgtgga cgacgccgca tcgctcgccg gctcggcggt
     1861 gcgacacctt tcggcactcg cccacgtcag tggcggctgg accgacttct gggtcggggc
     1921 gggcacctcg acgtcgtggg tgacggggta tgccgggcac gccctcgggc gcgcggccta
     1981 ccatcgtggc ctgccgcgcg agctccgcgc cctggcgcac gccagcgcgg catcggctgt
     2041 cggcgcgctg gagcgccaac agcggcacgg cgcatggggc tggtccccgg cggtgcgggc
     2101 cgacgcggac tcgaccgcgt ggtgcgtccg tgccctggtc tcgatgggcc ggccggtcgg
     2161 ctcgcgcacg tgggacttcc ttgccggtca ccgaagcgcc gacggctacc ggacgtaccg
     2221 ccctaccgag cagaccggcc ggtgggccca gcccatgccc gaggtgacgg ccgtcgtcct
     2281 gctggcctac ctcgaggccg accggatcac gcccgccgag ctgggtgagc gatggctgca
     2341 gtggtgcggg ccgaccgggc cgtggacgag
//
LOCUS       DATAIX010000752         4276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_146200, whole genome shotgun sequence.
ACCESSION   DATAIX010000752 DATAIX010000000
VERSION     DATAIX010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146200"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1330
                     /gene="glp"
                     /locus_tag="VHM65_11100"
     CDS             71..1330
                     /gene="glp"
                     /locus_tag="VHM65_11100"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX2706297.1"
                     /translation="MLTVEEHLARVLATVTPGEPRHVPISAAAGLAVAETVTSKVDLP
                     GFDNSAMDGYAVRAPGVAGARAADPGSVVTLRVVGEVAAGADATIPVSPGTAVRIMTG
                     AMMPAGADAIVRVEDTDGGVDLVQIRQGAPAGTSIRARGEDVRAGEAVLDVGAVLDAR
                     RVALLAATGHGSVLVRPRPRVAVVSTGSELVPPGGTLGAGQIHDSNSYMLEALVRECG
                     AEPVRCDPLPDDAATLVAALHGLAEEVDVVVTSGGVSMGAYDVVKEGFQGRPGAGGDR
                     VEFVQVAMQPGKPQGFGVLGPRQVPFFALPGNPVSAYVSFVVFARPALRRMMGLTPEV
                     PEPLVATLATALRSPARRRQIARAVVRRDAEGLWADPVWGQASHFIADLSRANAFVVV
                     PEDVTLVEAGTPVEVWLLPGAEDRWPT"
     gene            1342..1839
                     /gene="moaC"
                     /locus_tag="VHM65_11105"
     CDS             1342..1839
                     /gene="moaC"
                     /locus_tag="VHM65_11105"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX2706298.1"
                     /translation="MTQEAVPHGTARLTHVRADGSAHMVDVSAKPVTTREASAAGRVL
                     LTADAVEALREGAVPKGDALAVARIAGIQAVKRTPELIPLAHPVAVHAVVVNLWVRDD
                     GVDVEATVRTADRTGIEMEALTAVAVAALALVDMVKAVDRHARITDVRVTAKSGGRSG
                     DWREP"
     gene            1836..2312
                     /locus_tag="VHM65_11110"
     CDS             1836..2312
                     /locus_tag="VHM65_11110"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX2706299.1"
                     /translation="MTRRGVVICCSNRAAAGVYEDRTGPLIVAALRSWGLETPDALVV
                     ADGPPVGAALAAALASGPQVLLTTGGTGLTPTDRTPEMTLPLLDRVVPGLAEAIRAAG
                     VRQGVATAILSRGVAGLAGRTLVVNLPGSTGGVRDALDVLAPVIAHAMDQIGGGDH"
     gene            2410..2967
                     /locus_tag="VHM65_11115"
     CDS             2410..2967
                     /locus_tag="VHM65_11115"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX2706300.1"
                     /translation="MIVLRPLRRRDRDEWFALRAANTGWTRPWEPSSPMGAVPQVSFT
                     RYVRSLDADGRTGASLTLAIEVGLQIIGQIQLFGVARGSLQSSAVGYWVAQHMGRQGI
                     APRAVAMVVDHAFGAMGLHRVEVNIRPDNVFSLRVVEKLGLRDEGLRERFLHIDGVWR
                     DHRSFALTADELGDRPLRDRLRGLP"
     gene            3480..>4276
                     /locus_tag="VHM65_11120"
     CDS             3480..>4276
                     /locus_tag="VHM65_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706301.1"
                     /translation="MQPSSLIFVVIIAIWAFYLLQHWVRRREYLATAHSVDRFSDAIR
                     VLEMHRETPSAVGPQGAPDDRPRSPLVARSSAASAAAPAWAASPTSPAPARSRLSHRG
                     PVRRVSRPPVRAVVGVLLLAALVGLPATVSLAVVGRLTWLGVVLDLAALLGLGLVLRR
                     AGVRRRAARRGLARRHTGGSSSVLDQASTSPGERSATGPAAEADVVVADPHAWTPVPV
                     PPPTYTLKAKAVYREPAPTPALVAAPAPAPTAAAAPGAGAATNAGVGA"
BASE COUNT          496 a         1560 c         1598 g          622 t
ORIGIN      
        1 gctgggcccc gacgccgcgg tgctgggcgc cgacgccgca ggtccggcct gacgtgcgga
       61 cggccctgat gtgctgaccg tcgaggagca cctcgcccgg gtcctggcga cggtcacgcc
      121 gggtgagccc cggcacgtgc cgatcagcgc ggccgctggg ctcgcggttg ccgagacggt
      181 gacctcgaag gtcgacctgc cgggcttcga caactccgcc atggacgggt atgccgtccg
      241 cgcacccggc gtggccggcg cccgagccgc cgaccccggg tccgtggtga cgctgcgggt
      301 cgtcggtgag gtggccgccg gggctgacgc gacgatcccg gtcagtcccg ggacggccgt
      361 gcggatcatg accggcgcga tgatgccggc cggcgccgat gcgatcgtca gggtcgagga
      421 caccgacggg ggagtcgatc tcgtgcagat ccgccagggt gcgccggccg ggacatcgat
      481 ccgcgcgcgg ggcgaggacg tcagggccgg cgaggccgtc ctcgacgtcg gggccgttct
      541 cgacgcccgg cgggtcgcgc tgcttgcggc caccgggcac ggttccgtcc tcgtgcgccc
      601 tcgcccgcgt gtcgccgtcg tgtccaccgg cagcgagctc gtccccccgg gcgggaccct
      661 cggtgccggg cagatccacg actcgaacag ctacatgctc gaagccctcg tccgcgagtg
      721 cggggcggag cccgtccggt gcgaccccct gcccgacgac gcggccacgc tcgtcgcggc
      781 gctgcatggg ctcgccgaag aggtcgacgt cgtcgtcacg tcgggagggg tgagcatggg
      841 tgcctatgac gtcgtcaagg agggcttcca gggccggccg ggcgccggtg gcgaccgcgt
      901 cgagttcgtc caggtcgcga tgcagcccgg caagccgcag ggcttcgggg tcctcggccc
      961 gcggcaggtg cccttcttcg ccctccccgg caacccggtc tcggcctacg tgtcgttcgt
     1021 cgtcttcgcc cgtccggcct tgcggcggat gatgggcctg acccccgagg tgcccgagcc
     1081 gctggtggcg acgctggcga cggcgctgcg ctcgccggcc cggcgtcgcc agatcgcccg
     1141 ggccgtcgtg cgacgcgacg ccgaggggct ctgggccgat ccggtgtggg gacaggcctc
     1201 gcacttcatc gccgatctgt cgcgggccaa cgccttcgtc gtcgtgcccg aggacgtaac
     1261 cctcgtcgag gcgggcacgc cggtcgaggt gtggctcctc cccggcgccg aggaccggtg
     1321 gccgacgtga gtgaggctga catgacgcag gaagcagtac cgcacgggac ggcgcggctc
     1381 acccatgtgc gggcggacgg gtccgcccac atggtcgatg tcagcgccaa gccggtgacc
     1441 acccgcgagg cgagcgcagc cggacgagtc ctgctcaccg ccgacgcggt cgaggcgctg
     1501 cgggagggcg cggttcccaa gggggatgcg ctcgcggtcg cgcgcatcgc gggtatccag
     1561 gcggtcaagc gcactccgga gctgatcccg ctggcgcatc cggtcgcggt ccacgcggtc
     1621 gtggtcaacc tgtgggtgcg cgacgacggc gtcgacgtcg aggcgacggt gcggacggcg
     1681 gaccggacgg ggatcgagat ggaggcgctg acggcggtcg ctgtggccgc gctcgcgctc
     1741 gtcgacatgg tcaaggcggt ggaccgccat gcccggatca ccgacgtgcg ggtgaccgcc
     1801 aagtccggtg gccgctccgg ggactggcgc gagccgtgac ccgccgtggg gttgtcatct
     1861 gctgctcgaa ccgggccgcc gcgggagtgt acgaggaccg caccgggccg ctcatcgtgg
     1921 cggcgctgcg ctcctggggc ctcgagactc ctgacgcgct cgtcgtggcg gacgggcccc
     1981 cggtgggtgc ggccttggcg gcggcgctgg cctccggccc tcaggtgctc ctcaccaccg
     2041 gcggcacggg gctgacgccc accgaccgca cccccgagat gacactgccg ttgctcgacc
     2101 gggtcgtgcc gggcctcgcc gaggccatcc gggccgccgg ggtcaggcag ggcgtggcaa
     2161 ccgccatact ctcccggggg gtggccggac ttgccggacg gactctcgtg gtgaacctgc
     2221 ccgggtcgac cggcggcgtc cgcgatgccc tggacgtcct cgcgccggtc atcgcccacg
     2281 cgatggacca gatcggcgga ggggaccact gaccgtgacg gcggcacggc cccgtggggt
     2341 catcggcggc gggcgagtat gacgtcggcc tggccggtga cgctgacctc gcaggatggc
     2401 ctcgaccccg tgatcgtcct gagaccgctg cggcgccggg accgcgacga gtggttcgcc
     2461 ctgcgggcgg ccaacacggg ctggaccagg ccctgggagc cgtcctcgcc gatgggcgcg
     2521 gtcccccagg tgtcgttcac ccggtacgtg cggtcgctcg acgccgacgg gcggaccggg
     2581 gcgtcgctga cgctcgcgat cgaggtggga ctgcagatca tcgggcagat ccagctcttc
     2641 ggggtcgccc gtgggtcgct gcagtccagc gccgtcgggt actgggtggc ccagcacatg
     2701 ggccggcagg gcatcgcgcc gcgggccgtg gcgatggtgg tcgaccacgc gttcggggcc
     2761 atggggctgc accgggtcga ggtcaatatc cggccggaca acgtcttcag cctgcgcgtc
     2821 gtggagaagc tgggcctgcg cgacgagggg ctgcgcgagc gcttcctgca catcgacggg
     2881 gtctggcgcg accatcgcag ctttgccctg accgccgacg agctcggtga ccgcccactg
     2941 cgtgaccgcc tgcgcggcct gccgtgatgc tgttgatgcc gtgatgcccg tgataagcac
     3001 tacccctcgc cgcgaccgcg gtggctacgg cgatcccctc gtccgctcgg cgccggcccg
     3061 aggggcgtcg gcggcgggac caggcaggtg cattccggct cctatgccgt gcccgccgct
     3121 ggcgggctcg gtccctatgc cgtgcccgcc gctggcgcac ggcacagctt gcgtcacggt
     3181 ataggcccag ccccgtgccc gccgctggcg ggcacggcat agcttgcgtc acggcatggg
     3241 gccagccccg tgcccgccgc tggcgggcac cgcatacgtc gcgcacggca tagcttgcgt
     3301 cacggcgtgg ggccagcccg gtgcccgccg ctggcgggca ccgcatacgt cgcgccgggg
     3361 gatagacgac gttgagaccg ggccgatcgg cgttttcaca ccagtaacag cagcctcatt
     3421 ggcgacacac cgtgccaggt ccgggacttg gccccggcca ccgcctagcg ttttgccccg
     3481 tgcagcccag ctccctgatc ttcgttgtca tcatcgcgat ctgggctttc tacctgctcc
     3541 agcactgggt tcgtcgccgg gagtacctcg ccaccgcgca ctcggtcgac cgcttctccg
     3601 acgcgatccg ggtgctcgag atgcaccgtg agacgccgtc ggcggtcggg ccacagggtg
     3661 cccccgatga tcggccacgc tccccgctcg tcgcccggtc atcggcggcc tcggcggccg
     3721 cgccggcctg ggcggcctcc ccgacctcgc cggccccggc tcgttcacgc ctctcgcatc
     3781 gggggccggt ccggcgagtc tcgcgcccgc cggtgcgagc cgtcgtcggc gtcctcctcc
     3841 tcgcagccct ggtgggcctg ccggccacgg tctcgctggc tgtcgtcggg cggctcacct
     3901 ggctcggtgt ggtgctcgac ctagcggcgc tcctgggact gggcctggtg ctccggcgtg
     3961 cgggcgtgcg tcgtcgtgcg gcgcgacgcg gtcttgcgcg ccgtcataca ggagggtctt
     4021 cctccgtcct cgaccaggca agcacatccc caggtgagcg aagcgcgacc ggccccgcag
     4081 cagaggccga cgtggttgtc gctgaccccc acgcatggac gccggtcccg gtgccgccac
     4141 cgacctacac gctcaaggcc aaggcggtct accgcgaacc cgctcccact cccgcattgg
     4201 tggccgcacc ggccccggcg cccacggccg ccgccgcgcc cggggccggt gcggccacca
     4261 atgcgggagt gggagc
//
LOCUS       DATAIX010000753         2304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_146256, whole genome shotgun sequence.
ACCESSION   DATAIX010000753 DATAIX010000000
VERSION     DATAIX010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146256"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VHM65_11125"
     CDS             <1..308
                     /locus_tag="VHM65_11125"
                     /inference="COORDINATES: protein motif:HMM:NF024037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706302.1"
                     /translation="WAQSTRELTCPAVDLQAECTVLLTLTTTAPPDSPSPVTDLGYPL
                     HKHPARVQPLPVTAGTAHFFCPEATAERCTAALVLHIDPIALVGRRTFRDSDRQPLG"
     gene            complement(738..1607)
                     /locus_tag="VHM65_11130"
     CDS             complement(738..1607)
                     /locus_tag="VHM65_11130"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2706303.1"
                     /translation="MSVAERVRPAPAPRTAGVLRASLRLLGSEMRIISGRRRNQAGLL
                     VLASVPVLIAVAVKMSAPRRGRGPDFLSSITSNGIFVALTALTIEVGLFLPLAVAALA
                     GDTVAGEANTGTLRYLLTVPVHRTRLLLVKYASLVLGACIGVVVVALTGVLIGAALFG
                     LGPMTLLSGSQIGLADAFGRLGIVVLYLSCGLAALAAVGLFISTLTEQPIGAMIAVTI
                     VSTAMWILDGIAQLEWLHPWLLVHDWLSFSDVFRDPVFWDAMRQGLLVDLGYVVVFLA
                     LAWARFAQKDITS"
     gene            complement(1594..>2304)
                     /locus_tag="VHM65_11135"
     CDS             complement(1594..>2304)
                     /locus_tag="VHM65_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2706304.1"
                     /translation="AVLPRVGSLVEGPAFHPYLSGRANLARLDAADRLADPATSRIRI
                     DAALDRVGLLAAARKRYRAYSLGMRQRLALAAALLQPRDLLILDEPTNGLDPQGTREV
                     RSLIAGVAAEGATVLVSSHLLSEVEQICSHLGIMREGALVAQGTVAEVQAGSRPVAEV
                     TTGSPGQAAEVLRSLGLTEVTSTTTGVRAIPGDVDPERIVAALVGAGVGVRGFSVKAP
                     SLEELFVSLTGEGFDVSR"
BASE COUNT          365 a          858 c          804 g          277 t
ORIGIN      
        1 tctgggccca gagtacgagg gaactcacgt gccccgcggt cgacctccag gcagaatgca
       61 cggtgctcct caccctgacg acgaccgcgc caccggactc cccgagtccg gtgaccgacc
      121 tcggataccc cctgcacaag cacccggccc gggtccagcc tctccccgtc acggccggca
      181 cggcccattt cttctgcccc gaggccactg ccgagcgctg caccgccgcg cttgtgctcc
      241 acatcgaccc gatcgccctc gtcggtcgcc ggacgttccg cgacagcgac cggcagcccc
      301 tcgggtagta cgtcaacgac cgcccgtgtg ccgggtcctc cttgctctcg gcgggccctg
      361 ggcgcgatct tccgcatggc cataggcggg cactgcgacg ccaaccagga cctgcccggg
      421 catacccctg gccgttggac gtccaggtgc ctgcggtgcc gtgccgtgcc gcggcggcgc
      481 agcgctggcg gcgcagccct ggcggtgcgg ctcgtcaccc cgctcggctg gacggtcgag
      541 gccaccccga tgcgctgacc cgcctttacg tgctgctcca ggtgctcgag gacgccaagc
      601 actactgggt gtccgaggac gaggtcgaca cgctgctcgc tggggcgcgg tccaccagac
      661 cgcgtttgcg gtcctcgcgc tggagtccga gcccgtcgac ccccggccgt aggaggccgc
      721 cgcccggcgt cgccggccta gctcgtgatg tccttctggg cgaaccgggc ccaggccagg
      781 gcgaggaaga cgacgacata cccgaggtcg acgagcagcc cctgacgcat ggcgtcccag
      841 aagaccgggt cgcggaagac gtccgagaac gagagccagt cgtggacgag cagccacggg
      901 tgcagccact cgagctgggc gatcccgtca aggatccaca tcgcggtcga cacgatcgtc
      961 acggcgatca tcgccccgat cggctgctcg gtcagcgtcg agatgaacag gccgaccgca
     1021 gccagggccg cgagcccgca gctgagatag agcacgacga tcccgagccg cccgaacgcg
     1081 tcggccagcc cgatctggct cccggagagc agggtcatcg gcccgagccc gaagagcgcc
     1141 gccccgatga gcaccccggt cagcgcgacg acgaccaccc cgatgcaggc cccgagcacg
     1201 agcgatgcgt acttcacaag cagcagccgg gtccggtgca cgggcacggt cagcaggtag
     1261 cgcagggtcc ccgtgttggc ctcccccgcg acggtgtcac ccgcgagcgc cgcgaccgcg
     1321 aggggcagga acagcccgac ctcgatcgtc agcgcggtga gcgcgacgaa gatcccgttc
     1381 gaggtgatcg aggacaggaa gtccggcccg cggccgcggc ggggggccga catcttgacg
     1441 gcgacggcga tgagcacggg cacggaggcg agcacgagca ggccggcctg gttgcgccgc
     1501 cgcccggaga tgatccgcat ctccgaaccg agcaggcgca acgacgcccg gagcaccccg
     1561 gccgtccgcg gcgcgggtgc cggacgcacg cgctcagcga ctgacatcga agccctcccc
     1621 cgtcagcgag acgaagagct cctcgaggct aggtgccttc acggagaacc cgcgcacgcc
     1681 caccccggca ccgacgagag cggcgacgat ccgttcaggg tcaacatccc ctggtatggc
     1741 gcgcaccccc gtcgtcgtcg acgtcacctc ggtcaggccc aggctgcgca gcacctcggc
     1801 ggcctggccc ggcgacccgg tcgtcacctc ggcgaccggc cgtgatccgg cctggacctc
     1861 ggccacggtg ccctgggcga ccagcgcgcc ctcgcgcatg atcccgaggt gggagcagat
     1921 ctgctcgacc tccgagagca gatggctcga cacgaggacg gtcgccccct cggcggcgac
     1981 cccggcaatg agcgagcgga cctcacgagt cccttgcggg tcaaggccgt tcgtcggttc
     2041 gtcgaggatg agcaggtcac gcggctggag cagtgcggcg gcgagcgcga gccgctgacg
     2101 catcccgagg gagtaggcgc gatagcgctt gcgagccgcc gcgagcagcc cgacccggtc
     2161 cagcgcggca tcgatccgga tccgggaggt cgccgggtcg gcgagccggt cggcggcgtc
     2221 gagccgggcc aggttggccc ggccggacag gtacgggtgg aaggccgggc cctcgacgag
     2281 cgacccgacg cgcggcagga ccgc
//
LOCUS       DATAIX010000754         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_265963, whole genome shotgun sequence.
ACCESSION   DATAIX010000754 DATAIX010000000
VERSION     DATAIX010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265963"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VHM65_11140"
     CDS             complement(<1..516)
                     /locus_tag="VHM65_11140"
                     /inference="COORDINATES: protein motif:HMM:NF017046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEX2706305.1"
                     /translation="MTSERHVKTHHPRPTTDELRAFIARGWSPRDEAPPERATVAPYT
                     AARRERVSAAFPGERLVIPAGGLTVRSNDTDYVFRPHSAFAHLTGIGADREPDAVLVL
                     EPMGEGGHEAVLFFRPLADRDSDEFFADTRYGEFWVGARPTIADVEAELGISARHIEE
                     LAGAIAKDVGVV"
     gene            complement(563..>2198)
                     /locus_tag="VHM65_11145"
     CDS             complement(563..>2198)
                     /locus_tag="VHM65_11145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEX2706306.1"
                     /translation="DVLVVGGGVTGTGCALDAVTRGLATGLVEQRDFASGTSSRSSKL
                     IHGGLRYLEMFDFRLVAEALKERGLLLTRLAPHLVRPVPFLYPLTKPLIERPYVEAGL
                     TLYDTMARGSRHSAGLDWHKHLSRRQALKIAPALKRSALVGAVYYFDAKVDDARHTME
                     IARTAATYGALVASRVQVVGFLRQGERVTGVKALDKVSGHEFDIRARHVVNATGVWTD
                     DTQALIGERGQFHVRASKGIHLVVPRDRIHSTAGIILRTEKSVLFVIPWGRHWIIGTT
                     DTGWDLDKAHPAATSADIDYLLHHVNTVLTTSLTQADVEGVYAGLRPLLAGESEETSK
                     LSREHVVAHPTPGLVVVAGGKYTTYRIMAKDAIDEAARALGGDVPASVTEDIPMVGAV
                     GYQAMWNRRRAMACESGLHVERIEKMLMRHGVLTTEVLDLVAADRSLGEPLPGGEDYL
                     RAEIVYAASHEGALHLDDVLARRTRLSIESFDRAITASRPAAELVAPILGWDAAMIDE
                     EVEHYRQRVEAERVSQTMPDDASADAARLAAPDITG"
BASE COUNT          302 a          723 c          845 g          328 t
ORIGIN      
        1 gacaacccca acgtccttgg cgatagcacc ggcgagctcc tcgatgtgcc gggcgctgat
       61 ccccagctcg gcctcgacat cggcgatcgt cggccgggcc ccgacccaga actcgccata
      121 gcgggtgtct gcgaagaact cgtccgagtc gcggtcggcg aggggccgga agaacagcac
      181 cgcctcatgc ccaccctcgc ccatgggttc gaggaccagg actgcgtcag gctcgcggtc
      241 tgcgccgatg ccggtgaggt gggcgaacgc gctgtgcggg cggaagacat agtcggtgtc
      301 gttgctgcgg accgtcaagc ccccagccgg tatgacgagc cgctcacccg ggaaggcggc
      361 ggagacgcgc tcgcggcggg cggcggtata cggggcaacc gtggcccgct cgggcggagc
      421 ctcgtcgcgg ggtgaccagc cgcgggcgat gaaggcccgg agctcgtccg tcgtcggcct
      481 cgggtggtgg gtcttgacgt gccgctcgct agtcacgcac accatggtgc cattgccagg
      541 gcgcggctcc cgtctgcccg ggtcagccgg tgatgtccgg ggcggctagg cgtgcggcgt
      601 cggccgaggc gtcgtcgggc atcgtctggg acacccgctc ggcctcgacc cgctggcggt
      661 agtgctcgac ctcctcgtcg atcatcgcgg cgtcccagcc gaggatcggg gcgacgagct
      721 cggccgccgg gcgtgaggcg gtgatggccc ggtcgaagga ctcgatggac agccgggtgc
      781 gccgggcgag cacgtcgtcg aggtgcagcg ccccctcgtg agaggcggca tacacgatct
      841 cggcccgcag gtagtcctca ccgccgggga gcggctcgcc gaggctgcgg tccgccgcga
      901 cgaggtcgag gacctcggtc gtcaggacgc cgtggcgcat gagcatcttc tcgatccgtt
      961 cgacgtgcag gccggactcg caggccatgg cgcggcggcg gttccacatc gcctggtagc
     1021 cgacggcccc gaccatcggg atgtcctcgg tcaccgacgc cgggacgtcg ccgccgagcg
     1081 cgcgcgcggc ctcgtcgatg gcgtccttgg ccatgatccg gtatgtcgtg tacttgccgc
     1141 ccgcgacgac gaccagcccc ggcgtggggt gggccaccac gtgctcacgg gagagcttgc
     1201 tcgtttcctc gctctcgccg gcgagcagcg gccgcagtcc ggcatacacg ccctcgacgt
     1261 cggcctgggt caggctcgtc gtgaggaccg tgttgacgtg gtgcaggagg tagtcgatgt
     1321 cggcggacgt cgcggccggg tgggccttgt cgaggtccca cccggtgtcg gtcgtgccga
     1381 tgatccagtg ccgcccccag gggatgacga acaggaccga cttctcggtg cgcaagatga
     1441 tgcccgcggt cgagtggatc cggtcgcggg gcacgacgag gtggatgccc ttggaggcgc
     1501 ggacgtggaa ctgaccgcgc tcgccgatga gggcctgcgt gtcgtcggtc cagacgccgg
     1561 tggcgttgac gacgtggcgg gcgcggatgt cgaactcgtg ccccgacacc ttgtccagcg
     1621 ccttgacgcc cgtgacccgc tcgccctggc gcaggaaccc gacgacctgg acgcgcgagg
     1681 cgacgagcgc gccataggtg gccgcggtgc gggcgatctc catggtgtgc cgcgcgtcgt
     1741 cgaccttggc gtcgaagtag tagacggctc cgacgagagc cgaccgtttg agggcggggg
     1801 cgatcttgag ggcctggcgc cggctcaggt gcttgtgcca gtccagcccg gccgagtgcc
     1861 gggagccgcg ggccatcgtg tcgtagaggg tgaggcctgc ctcgacgtac gggcgttcga
     1921 tgaggggctt ggtcaggggg tagaggaagg ggacgggccg gacgaggtgc ggagccaggc
     1981 gggtcagcag caggccccgc tccttgaggg cctcggcgac gaggcggaag tcgaacatct
     2041 ccaggtagcg caggccgccg tggatgagct tgctcgagcg gctcgacgtg ccggaggcga
     2101 agtcgcgctg ctcgaccagg ccagtcgcca ggcctcgggt gaccgcgtcg agggcgcatc
     2161 cggtgccggt cacgccaccg ccgacgacga ggacgtcg
//
LOCUS       DATAIX010000755         2390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_265995, whole genome shotgun sequence.
ACCESSION   DATAIX010000755 DATAIX010000000
VERSION     DATAIX010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265995"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..243
                     /locus_tag="VHM65_11150"
     CDS             13..243
                     /locus_tag="VHM65_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706307.1"
                     /translation="MTSVLVRDLPEETHRELLRRAEERGQSLQQYLAAELTRLAASPT
                     LGDVLRRIDTRRGGRVGLAQAAADLADERSRS"
     gene            240..671
                     /locus_tag="VHM65_11155"
     CDS             240..671
                     /locus_tag="VHM65_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX2706308.1"
                     /translation="MIVVDASVLANAIGDDAEAGRQARDLLREHREVAAPDLVDVETT
                     AVLRRRWLAGTLTDERFEQAVDDLADIPLARFPARGLMRRAFELRAKVTAYDACYVAL
                     AEALGWPLCTADRRLTRATGPRCTMHLIAPPATRTGPPGSG"
     gene            complement(640..1062)
                     /gene="msrB"
                     /locus_tag="VHM65_11160"
     CDS             complement(640..1062)
                     /gene="msrB"
                     /locus_tag="VHM65_11160"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX2706309.1"
                     /translation="MDESRTYPLAKTDEQWRAELTPAEYHVLREAGTERAFVGQYTDT
                     KTVGVYRCRACSTELFRSETKFESHCGWPSFYAPLAEDRVDYLEDRSGLGPPRVEVRC
                     ATCGSHLGHVFEGEGYGTPTDQRYCINSISLTLEGQSG"
     gene            1197..2258
                     /gene="ligD"
                     /locus_tag="VHM65_11165"
     CDS             1197..2258
                     /gene="ligD"
                     /locus_tag="VHM65_11165"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX2706310.1"
                     /translation="MADSAKVLEVPGPDDQMRAVRISSPDRVLWPDEGITKLELATYL
                     CEVGEPFVRIVGNRPVTLQRFPTGIGGEEFYSKNPPRGVPGYVRTVMCTYPSGRRHPQ
                     LVVDEVAAAIWAVQMNTITFHPWPVLAGPEPRSLDNPDELRIDLDPQPGRGFADAVAA
                     AYGLRSVLADAGLVGYVKTSGNRGVHVHCRIAPVHEFLDVRHGVIAIARELERRMPDL
                     VTTSWWKEERGEKVFVDFNQACRDRTIASAYSPRPLPGAPVSTPVSWDDLGSVDPREL
                     TVRTVPGVVAAHGDAWASIGSQVGDVATALQWWERDLGDGLGELSFPPDYPKMPGEPP
                     RVQPSRKRRADEDYDSPPG"
BASE COUNT          323 a          836 c          855 g          376 t
ORIGIN      
        1 cataatgcaa gcatgactag tgttcttgtt cgtgacctcc cggaggagac acaccgagag
       61 ctgctccggc gcgcagaaga gcgcggccag tcccttcagc agtacctggc cgccgagctg
      121 acccggctcg cggcgtcacc caccctgggc gacgtcctgc ggcgcatcga cacccgccgc
      181 ggcggccggg tcgggctggc tcaggcggca gcggaccttg ccgacgaacg ctcccgctcg
      241 tgatcgtcgt cgacgcctct gtgctggcca acgccatcgg cgatgacgca gaggcgggac
      301 ggcaagcccg cgacctgctg cgcgagcacc gagaggtggc cgcgcccgac ctggtcgatg
      361 tggagacgac cgccgtcctc cgacggcgat ggctggccgg tacgctcact gacgagcgtt
      421 tcgagcaggc tgtcgatgac ctcgccgaca tcccgctcgc cagattcccc gctcgcggac
      481 tgatgcgccg agcgttcgag ctgcgcgcca aagtcaccgc ctacgatgcc tgctacgtcg
      541 ctcttgccga ggcgctgggc tggccgctgt gcacggccga ccgccgcctc actcgtgcca
      601 ccggtccacg gtgcaccatg cacctgattg cgccgccggc tacccggact ggccctccag
      661 ggtcaggctg atcgagttga tgcagtagcg ctggtcggtc ggcgtgccgt agccctcgcc
      721 ctcgaagacg tggccgagat gcgacccgca cgtggcgcac cgcacctcga cgcgcggcgg
      781 ccccaggccg gagcggtcct cgaggtagtc gacccggtcc tcggcgagcg gcgcatagaa
      841 cgagggccat ccgcagtgcg actcgaactt cgtctcgctg cggaacagct cggtgctgca
      901 ggcccggcac cgatacacgc caacggtctt ggtgtcggtg tactggccca cgaatgcccg
      961 ctcggtgccg gcctcgcgca gcacgtggta ctccgccggg gtcagctcgg cgcgccactg
     1021 ctcgtcggtc ttggccaagg ggtaggtccg cgactcgtcc atagagggtc cctttctgcg
     1081 gatccgctgg atgcggcgcc agatgtccac accagcacaa ccccctgggg tccgcggacg
     1141 ttcccggtcc gcggcccggg ttagtctcgg gttggtcccg ggctagtctc ggggccatgg
     1201 ccgactcagc gaaggtcctt gaggtgccgg gtcctgacga ccagatgcgc gcggtgcgga
     1261 tctcgagtcc cgaccgggtg ctctggccgg acgaggggat caccaagctc gagctcgcga
     1321 cctacctgtg cgaggtgggg gagccgttcg tccggatcgt cgggaaccgt ccggtgacgc
     1381 tgcagcgctt cccgaccggg atcggcgggg aggagttcta ctccaagaac ccgccgcgag
     1441 gcgtgccggg ctatgtgcgc accgtcatgt gcacctaccc ctcgggccgg cgccacccgc
     1501 agctcgtcgt cgacgaggtg gcagccgcga tctgggcggt ccagatgaac accatcacgt
     1561 tccacccgtg gcccgttctc gccggtcccg agccacgttc gctcgacaac cccgacgagc
     1621 tgcggatcga cctcgacccg cagccggggc gtggcttcgc cgacgcggtg gcggcggcat
     1681 acgggctgcg ctcggtgctg gccgacgcgg ggctggtggg ctacgtcaag acctcgggca
     1741 accgcggggt ccatgtgcac tgccggatcg cgccggtcca cgagttcctc gacgtgcgcc
     1801 atggcgtcat cgccatcgcg cgcgagctcg agcggcgtat gccggacctc gtcacgacct
     1861 cgtggtggaa ggaggagcgc ggcgagaagg tgttcgtcga cttcaaccag gcctgtcgcg
     1921 accggacgat tgcctcggcc tacagcccgc ggccgctgcc cggtgcgccg gtgtcgacgc
     1981 cggtctcctg ggacgacctc gggtcggtcg accctcgcga gctgaccgtc cggacggtgc
     2041 cgggggtcgt tgcggcacac ggggatgcgt gggcctcgat cgggtcgcag gtcggtgacg
     2101 tcgcgacggc gctgcagtgg tgggagcggg acctcggcga cggccttggc gagctgagct
     2161 tccctcccga ctaccccaag atgccgggcg agccgccccg ggtgcagccc tcgcgcaagc
     2221 gccgcgccga cgaggactac gactctccgc ctgggtgact ccgcctggga tcggcaccgg
     2281 ccgggtcggc ctcggtctgg cgggcgccgg tagtgaggtg gttctggccg gtgggggtcg
     2341 gccggatcgg cctcggtctg gcgggcagcg ggtagtgagg tggttctggc
//
LOCUS       DATAIX010000756         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_266014, whole genome shotgun sequence.
ACCESSION   DATAIX010000756 DATAIX010000000
VERSION     DATAIX010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266014"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..819)
                     /locus_tag="VHM65_11170"
     CDS             complement(202..819)
                     /locus_tag="VHM65_11170"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX2706311.1"
                     /translation="MTTAHPARRALVLVDLQNDFCEGGSLPVSGGAGVAALISDYVTS
                     QAGAYDAIVATADWHEDPGAHFAADPDYVDTWPAHCRADTPGADFHPGAAGALAHVEA
                     VFRKGRHAAAYSGFEGITAEGDDTVALAEWLAERDITAVDVAGIATDHCVRATALDAL
                     AEGFRTRVLLDLTAGVARESTERALVQLREAGAELVGTPVVSRRP"
     gene            1014..2048
                     /locus_tag="VHM65_11175"
     CDS             1014..2048
                     /locus_tag="VHM65_11175"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX2706312.1"
                     /translation="MNTLRVVSIVAIALVILFDYTNGFHDASNIIATVIASRAMTPIQ
                     AVIVVGTFEFLGPVLGGTAVANTIGKFVDVSTLDPQRAVTVILCGIVGAIAWNLGTWR
                     VGIPSSSSHALVGGLVGAVVVAAGFDYVLWGFAELAKGHVTGVTKVLLGLILSPLIGF
                     WLGFLIHRLMSALLQRAKPVANTYLRRVQWVTSAGLAFSHGANDAQKSMGIITMILVL
                     SGSLPGFAVPFWVILSCAAAITLGILSGGWRIVRTVGFGIYKVRPLHAFDAQLTSAAV
                     IFAASTLGAPVSTTHVVSSSIMGIGSSERPRSVRWAKAQEIVTTWLVTIPGSAAVAIV
                     VYLVTSLFTR"
     gene            2065..2742
                     /locus_tag="VHM65_11180"
     CDS             2065..2742
                     /locus_tag="VHM65_11180"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX2706313.1"
                     /translation="MMSQLMGRVFPRMPDFFGMLNNQCALAVDGLDALVAYMHDGTHE
                     AGMGVRLLEHEGDEAKGRHMDALNKAFSTPMDREDLYRAISTLDHVLNYAKTTVREME
                     MLGVDPDAHTLEMAGFLREGTIALRDGYAKLSTVPAEAEEGAQAARKAERNIEKAYRR
                     ALADLFNAESEVAAMTTIDGHSGGAALTHVMEVFKKREIYRHLSNAGDRVARAGEVLH
                     DIVVKMV"
BASE COUNT          419 a          978 c          938 g          431 t
ORIGIN      
        1 tcccaagcca ccgtgacgcg gtcatcctca cccagcgtgg cggctaacac caaccggatc
       61 tgtcgtcggc aatgcctaga tgtcgatgcg cgtggcggcc cgatcgtggg gatagtctcc
      121 gtcaccgagc gagagcccgc caggccgggc cggcgtgtca cccgagcccc accgcaggca
      181 tgaagagttg aggcatctca ttcacgggcg ccgtgagacc acgggggtgc cgacgagctc
      241 ggccccggcc tcgcggagct gcaccagggc gcgttcggtg ctctcgcgcg cgacgcccgc
      301 ggtgaggtcg agcagaaccc gggtccggaa cccttcggcg agcgcgtcga gcgccgtcgc
      361 ccgcacgcag tggtccgtcg cgatcccggc gacgtcgacg gcggtgatgt cgcgctcggc
      421 gagccactcg gccagagcca ccgtgtcgtc gccctcggcg gtgatcccct cgaacccgct
      481 gtatgccgcg gcgtggcgcc ccttgcggaa caccgcctcg acgtgggcga gggcccccgc
      541 ggcgccgggg tggaagtcgg cgccgggggt gtcggcgcgg cagtgggcgg gccaggtgtc
      601 gacgtagtcg gggtcggcgg cgaagtgggc gccggggtcc tcgtgccagt ccgcggtggc
      661 gacgatcgcg tcatacgcgc cggcctggct ggtgacgtag tcgctgatca gggcggccac
      721 gcccgcccct ccggagaccg ggagcgaccc gccttcgcag aagtcgttct ggaggtcgac
      781 gaggacgagg gcgcgcctcg ccgggtgcgc ggtggtcatg gtccagacgg tacgcgacca
      841 gggacctcac cgagccgccc aggggaccaa tgtcctcagc cacggtctgg ctctacccta
      901 aggttacgca aaagcagcgc tgcggtgaac tgagggtgaa taccgggcgc tggctgtcag
      961 gacccgaaac cacagcgggc aaaggagcct tgcatgggca cggacgtgag ccggtgaaca
     1021 ccctgcgggt ggtgtcgatc gtcgcgatcg ccctggtcat cctgttcgac tacaccaacg
     1081 gcttccacga cgcgtccaac atcatcgcca ccgtcatcgc ctcgcgggcc atgaccccga
     1141 tccaggcggt catcgtcgtc gggaccttcg agttccttgg ccccgtgctc ggtggcacgg
     1201 cggtggccaa caccatcggc aagttcgtcg acgtgagcac cctggaccca cagcgtgcgg
     1261 tgaccgtgat cctgtgcggc atcgtcggcg ccatcgcgtg gaacctcggc acctggcggg
     1321 tcggcatccc gtcgtcgtcc tcgcacgccc tcgtcggcgg cctggtcgga gccgtcgtcg
     1381 tcgcggcggg cttcgactac gtcctgtggg gcttcgccga gctggcgaaa ggccacgtca
     1441 ccggcgtcac gaaggtgctc ctcggtctga tcctctcgcc gctcatcggc ttctggctgg
     1501 gcttcctcat ccaccggctc atgagcgccc tgctgcagcg ggccaaaccc gtcgccaaca
     1561 cctacctgcg ccgagtccag tgggtcacct cggccgggct ggccttctcc cacggcgcca
     1621 acgacgccca gaagagcatg gggatcatca cgatgatcct cgtgctgtcc gggtcactcc
     1681 ccggtttcgc agtgcccttc tgggtcatcc tcagctgtgc ggccgcgatc accctgggca
     1741 tcctgtccgg cggctggcgc atcgtgcgga cggtgggctt cgggatctac aaggtccggc
     1801 cgctgcacgc cttcgacgcc cagctgacct cggccgcagt catcttcgcc gcgtcgaccc
     1861 tcggcgcgcc cgtctcgacg acccacgtcg tcagctcctc gatcatgggc atcggctcct
     1921 cggagcggcc caggtcggtg cgctgggcca aggcccagga gatcgtcacg acctggctgg
     1981 tcacgatccc cggcagcgct gccgtggcga tcgtggtcta cctggtcacc tcgctgttca
     2041 cccgctgatg gaaaggacga cgccatgatg tcgcagctga tgggccgggt cttcccccgg
     2101 atgccggact tcttcggcat gctcaacaac cagtgcgcgt tggccgtcga cggcctggat
     2161 gctctggtcg cctacatgca cgacggaacc cacgaggcgg gcatgggagt gcgcctgctc
     2221 gagcacgagg gggacgaggc caagggccgt cacatggacg cgctcaacaa ggcgttctcg
     2281 accccgatgg accgcgagga cctctaccgg gcgatcagca cgctcgacca cgtcctcaac
     2341 tacgccaaga cgaccgtgcg cgagatggag atgctcggcg tcgaccccga cgcgcacacc
     2401 ctggagatgg ccgggttcct ccgggagggc accatcgccc tgcgtgacgg ctatgccaag
     2461 ctctcgactg tcccggccga ggccgaggag ggcgcccaag ccgcccgcaa ggccgagcgc
     2521 aacatcgaga aggcctaccg gcgggccctg gccgacctgt tcaacgccga gtccgaggtg
     2581 gccgcgatga caaccatcga cgggcactcc gggggcgcgg ccctgaccca cgtcatggag
     2641 gtcttcaaga agcgcgagat ctaccgccat ctgtccaacg ccggtgaccg ggtcgcccgg
     2701 gccggcgagg tcctgcacga catcgtcgtc aagatggtct gagccgccca caggtccagt
     2761 gcgagc
//
LOCUS       DATAIX010000757         6202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_266056, whole genome shotgun sequence.
ACCESSION   DATAIX010000757 DATAIX010000000
VERSION     DATAIX010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266056"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1824)
                     /locus_tag="VHM65_11185"
     CDS             complement(<1..1824)
                     /locus_tag="VHM65_11185"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEX2706314.1"
                     /translation="MPDQPTPTDPMAAFGPNEWLVDEMYEQFKKDRHSVDKAWWEFFE
                     DYRPVDGGPMANGAPARLAPTPAVTPLAPAVAPSRPAPASAPKPRDIPARSAQAPRQE
                     DEVSPLKGPAARVVTNMEASLGVPTATSVRAVPAKLLIDNRVVLNNHLARSRGGKVSF
                     THLIGFAIVKAIARMPEMNFGYGQVDGKPVVVHPAHVNLGLAIDLAKPDGTRQLVVPS
                     IKACEGLDFVHFWTAYEDVVRKARSGKLVVDDFAGTTISLTNPGTIGTVHSVPRLMQG
                     QGAIVGVGAMDYPAAWQGASNETLNRNAVSKILTLTSTYDHRIIQGAQSGEFLRVIHQ
                     YLLGADRFYDEIFESLRIPYEPVRWVQDISATHDDDINKVARVQELIHAYRVRGHLMA
                     DTDPLEYRQRRHPDLDITSHALTLWDLDRHFATGGFGGEPFLKLRKILGILRDSYCRT
                     VGIEYMHIQDPEQRTWIQERIERTYAKTSRDEQLRILRRLNSAEAFETFLQTKFVGQK
                     RFSLEGGESVIALLDRILCRAAAENLDEVCIGMPHRGRLNVLANIAGKSYGQIFREFE
                     GTQDPKSVQGSGDVKYHLGQSGKFVSRRGSEIEVELAANPSH"
     gene            complement(2007..2855)
                     /locus_tag="VHM65_11190"
     CDS             complement(2007..2855)
                     /locus_tag="VHM65_11190"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2706315.1"
                     /translation="MSTVTLPLDAALPRAGLAAAVRSELRKFFTTRMWWGMAIGVFLA
                     GGLFALLFALLAGSTAGGGAGSSALPDLSDPQMVSTVYTGGLSVGYLLTLAVGVLTIG
                     SEYRHKTISSTFLSTPHRARVMGAKVISLLVIGAFYGLVSLTGSFGVGATVIAIKGFS
                     PAPDGGQLVRTMGLSLLVLGLWALIGLGVGILIPNQIVAILVAVGAAWIVEPLAGLAL
                     SFVSWGAKVVPYLPSQATSAMVEQVSQSDTVRLLSWWGGALVLTGYAAVMAVVGTWLT
                     LRRDVT"
     gene            complement(2852..3829)
                     /locus_tag="VHM65_11195"
     CDS             complement(2852..3829)
                     /locus_tag="VHM65_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2706316.1"
                     /translation="MTKAHDRRPPDSGAQIEVHDLTKKFGTLTAVDTLSFAVEPGRIT
                     GFLGPNGAGKTTTLRMILGLVRPTRGTATIGGHKYGELAAPSAMVGSALEATSFHPGR
                     SGRNHLRVLADAGGIPLERVDEMLDLVGIPAAANRRAGGYSMGMRQRLGLAAAMLGNP
                     RVLILDEPANGLDPEGIRWLREFLRYLAREGRTILISSHLLTEVDQTVDDVVIIANGE
                     LVTAGPIESLRGAPTLAVRTSKPAELAAALATQGVGSSPDPEGTLVVHSDDTHLVGDT
                     ALSIGAAVYELTRHRADLEDLYFRLTETPETRNRNLSSQAPALSEGAQQ"
     gene            3946..5292
                     /locus_tag="VHM65_11200"
     CDS             3946..5292
                     /locus_tag="VHM65_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX2706317.1"
                     /translation="MEWILVIAAVALTAGTALFVTAEFSLVALDRPTVQRAIDDGDGR
                     ARVVLASLKALSTQLSAAQVGITLTTLIVGYVAEPSIGALLTGPIDALPVPDSAVAGS
                     ATAAALVIATVFSMVFGELLPQFLGISAPLAAAKVVAMPLRVFAAVTKPLVRVLNGSA
                     NAFLRSLGIEPAEELSGARTPQELASLVRRSAQAGTLDAGTARMLTRSLDFGDRTAAD
                     VMTPRVRCTAVDRRDTGDDVVRLARSTGNSRFPVIGDDWDDIDGIIHVKRVIAVPQAR
                     RRDVPVSALMVDPVFVPETIRLPPLLRTLRQSGLQMAVVVDEYGGTSGVVTLEDVIEE
                     LVGEVDDEHDRPHSTGRRLTDGQWAVPGLWRPDEVRVRIGAVIPDGPTYETTGGYVMA
                     VLGRVPAVGDEVDIPGWRVRVAAMDGRRVDRLRFIPAPEPSGDAARDDTPARGASS"
     gene            5289..>6202
                     /locus_tag="VHM65_11205"
     CDS             5289..>6202
                     /locus_tag="VHM65_11205"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX2706318.1"
                     /translation="MSQTSALLVSLGLLLGNAFFVGAEFSAMAARRSSLEPLAESGNR
                     RAVVCLEALESMGSMLATAQLGITVCSVGLGALAEVALEQLLEPVLHSSGLASELAAP
                     IALGISLLIVVYLHVVVGEMIPKNLALAGPGQSALVLVPALLYLSRALRPFIRLMEWV
                     AKALVRLFGVEPRDEITSAFTAEEVAHIVVESHREGLIEGDRHGLVRRTLEFSDKVAR
                     DVAVPASALATIELGATPADIERLVARLGFSRFPVLDADGDVAGYLHVKDILYADDER
                     HTEPVPLKRVRRLATVSPGDEVEEVLAT"
BASE COUNT          845 a         2104 c         2264 g          989 t
ORIGIN      
        1 gtgcgacggg ttggccgcga gctcgacctc gatctcgctg ccgcgtcggc tcacgaactt
       61 gcccgactgg ccgaggtggt acttcacgtc gcccgagccc tggaccgact tggggtcctg
      121 ggtgccctcg aactcgcgga agatctggcc ataggacttg ccggcgatgt tggcgagcac
      181 gttgagccgg ccacggtgcg gcataccgat gcagacctcg tccaggttct cggcggcagc
      241 gcggcacagg atccggtcca gcagcgcgat caccgactcg ccaccctcga ggctgaatcg
      301 cttctgcccg acgaacttgg tctgcaggaa cgtctcgaac gcctcggcgg agttgagccg
      361 tcgcaggatc cgcagctgct cgtcacggga ggtcttcgcg taggtccgct cgatccgctc
      421 ctggatccac gtccgctgct cggggtcctg gatgtgcatg tactcgatgc cgacggtgcg
      481 gcagtaggag tcgcgcagga tgccgaggat cttgcgcagc ttgaggaacg gctcgccgcc
      541 gaaccctccg gtcgcgaagt gccggtctag gtcccacagg gtcagcgcgt gcgaggtgat
      601 gtcgaggtcg gggtgccggc gctggcggta ctccagcggg tcggtgtcgg ccatgaggtg
      661 gccgcggact cggtaggcgt ggatcagctc ttggacccgg gcgaccttgt tgatgtcgtc
      721 gtcgtgggtc gccgagatgt cctggaccca gcggaccggc tcgtacggga tgcgcagcga
      781 ctcgaagatc tcgtcgtaga accggtcggc gccgaggagg tactggtgga tgacgcgcag
      841 gaactccccg gactgggcgc cctggatgat gcggtggtcg taggtcgagg tcagggtgag
      901 gatcttgctc accgcgttgc ggttgagcgt ctcgttgctc gcgccctgcc aggccgccgg
      961 gtagtccatc gcaccgacgc cgacgatggc tccctggccc tgcatgaggc ggggaacgga
     1021 gtggaccgtg ccgatcgtgc ccgggttcgt cagcgagatc gtggtaccgg cgaagtcgtc
     1081 gacgacgagc ttgccgctgc gggccttgcg gacgacgtcc tcgtaggcgg tccagaagtg
     1141 gacgaagtcg agcccctcac acgccttgat gctcgggacg accagctggc gggtgccgtc
     1201 gggcttggcc aggtcgatcg cgagcccgag gttgacgtgg gccggatgga cgacgaccgg
     1261 cttgccgtcg acctggccgt acccgaagtt catctccggc atcctggcga tcgccttgac
     1321 gatcgcgaag ccgatgaggt gcgtgaacga caccttgccg cctcgcgagc gggcgaggtg
     1381 gttgttgagg acgacccggt tgtcgatgag cagcttggcg ggcacggccc gcacgctggt
     1441 cgccgtcggc accccgagcg aggcctccat gttggtgacg acgcgcgccg ccgggccctt
     1501 cagcgggctg acctcgtcct cctgccgggg ggcttgggca gaccgcgccg gtatgtcgcg
     1561 gggcttgggc gcgctcgccg gcgccggtcg cgagggagcc accgctggcg cgagcggcgt
     1621 cacagcgggt gtgggtgcca ggcgggccgg agcgccgttg gccatggggc cgccgtcaac
     1681 cggccggtag tcctcgaaga actcccacca cgccttgtcc acggagtgcc ggtccttctt
     1741 gaactgctcg tacatctcgt ccacgagcca ctcgttcggc ccgaaggcgg ccatcgggtc
     1801 cgtgggcgtg ggctggtccg gcacgtcggg ttcgcctctt tcgtcgcgtc agcgcgtggc
     1861 cggtgactgg gctcggcggc ctgcgcgagg agctgggtta cgggtccgtc cagactatcc
     1921 ccgatacccc gcccctggag tggccacggg gtcgccgcgt gctcggctga gcgctctgag
     1981 gacgggcacc tgcgcccgcc gggcggctag gtcacgtccc gccgcagggt gagccacgtc
     2041 ccgacgaccg ccatgaccgc cgcgtacccc gtgagcacga gcgcgccacc ccaccagctg
     2101 agcaaccgga cggtatcgga ctggctgacc tgctcgacca tggccgaggt cgcctggctc
     2161 ggcaggtagg gcacgacctt cgcgccccag gacacgaacg agagcgccag cccggcgagg
     2221 ggctcgacga tccacgccgc gccgaccgcg acgagaatgg cgacgatctg gttggggatg
     2281 aggatgccca cgccgagccc gatgagcgcc cagagcccga ggacgagcag cgacagtccc
     2341 attgtccgca cgagctgccc gccgtcggga gcgggcgaga accccttgat ggcgatcacg
     2401 gtggcgccga ctccgaacga cccggtgaga gacaccaggc cgtagaacgc gccgatgacg
     2461 agcaacgaga tgaccttggc gcccatgacg cgggcgcggt gcggggtgct gaggaacgtg
     2521 ctcgagatcg tcttgtgccg gtactccgac ccgatcgtca ggacaccgac ggcgagggtg
     2581 agcagatagc cgacgctgag cccgccggtg tagaccgtgc tgaccatctg ggggtcggac
     2641 aggtcgggca gcgcgctcga cccggccccg ccgcccgccg tgcttcccgc gagcaacgcg
     2701 aagagcaggg cgaagaggcc cccggcgagg aagacgccga tggccatgcc ccaccacatg
     2761 cgggtcgtga agaacttgcg cagctcggat cggacggcgg cggccagacc ggcccgcggc
     2821 agtgctgcgt cgagggggag ggtcacggtg ctcattgctg ggctccttcg gacagggcag
     2881 gggcctggct ggacaggttg cggttgcggg tctcgggtgt ctcggtgagg cggaagtaga
     2941 ggtcttcgag gtcggcgcgg tgccgggtga gctcgtagac ggctgcgccg atcgacaggg
     3001 ccgtgtcacc gacgaggtgg gtgtcgtcgc tgtggacgac cagcgtgccc tcggggtccg
     3061 gactcgaccc gacgccctgt gtcgcgagcg cggcagcgag ctcggccggc ttgctcgtcc
     3121 ggacggcgag cgtcggggcg ccgcgcagcg actcgatcgg gcccgccgtg acgagctcac
     3181 cgttggcgat gatgacgacg tcgtcgacgg tctggtcgac ctcggtcagc agatggctcg
     3241 agatgaggat cgtgcggccc tcgcgggcga ggtagcgcag gaactcgcgc agccaccgga
     3301 tgccttcggg gtcgagcccg ttggccggct cgtcgagaat caggacgcgg gggttgccga
     3361 gcatggcggc ggcgaggccg aggcgctggc gcatccccat cgagtaaccc cccgcccgac
     3421 ggttggccgc cgccgggatg ccgaccaggt cgagcatctc gtcgacccgc tccaggggga
     3481 tccccccggc gtcggcgagg acccgcaggt ggttgcggcc gctgcggccg gggtgaaagc
     3541 tcgtcgcctc cagcgccgac ccgaccatgg cgctcggggc cgccagctcg ccgtacttgt
     3601 gcccaccgat cgtggccgtg ccgcgtgtgg gacggaccag cccgaggatc atccgcagcg
     3661 tcgtggtctt gccggcgccg ttggggccga ggaagccggt aatgcggccc ggctcgacgg
     3721 cgaaggacag ggtgtcgacg gcggtgagcg tcccgaattt cttcgtgagg tcatggacct
     3781 cgatctgggc gccactgtcc ggtgggcggc ggtcgtgtgc tttcgtcatg ggtccatcaa
     3841 aacaaacgcc tgggcttccc accttcaccg agggggcgag ccggtgtggc gcgtgcctct
     3901 ccggctcggg gagagcgccc gcgcgaaccg taggatgggc ggactatgga gtggatcctc
     3961 gtgatcgccg ctgtggccct cacggcaggg accgcgctgt tcgtcacggc cgagttctcg
     4021 ctcgtggcgc tcgaccgccc gaccgtccag cgggccatcg acgacggtga cgggcgggcg
     4081 cgggtcgtcc tcgcctcgct caaggccctc tcgacccagc tctccgccgc ccaggtcggg
     4141 atcaccttga cgacgctcat cgtcgggtat gtcgctgagc cctcgatcgg ggcgctgctc
     4201 accgggccga tcgacgcgct gcccgtcccc gactcggccg tagccggctc ggccaccgcg
     4261 gcagccctgg tcatcgcgac ggtgttctcc atggtgttcg gcgagctgct gccccagttc
     4321 ctcgggatct cggcgccgct cgcggccgcc aaggtcgtcg ccatgcccct gcgcgtgttc
     4381 gcggctgtca ccaagccgct ggtccgggtc ctcaacggca gcgcgaacgc gttcctgcgc
     4441 agcctgggca tcgagccggc ggaggagctg tccggggcgc gcacgcctca ggagctcgcg
     4501 agcctggtcc gccgctcggc gcaggcgggg accctcgacg cggggactgc ccggatgctg
     4561 actcgctcac tcgacttcgg cgaccgcacc gccgccgatg tcatgacacc gcgtgtccgg
     4621 tgcaccgcgg tcgaccgcag ggacactggc gatgacgtcg tgaggctcgc ccggtcgacg
     4681 ggcaactcgc gtttcccggt catcggggac gactgggacg acatcgacgg gatcatccat
     4741 gtcaagcggg tcattgccgt accacaggcg cgacgccgtg acgtgcccgt gtcggcgctc
     4801 atggtcgacc cggtcttcgt ccccgagacg atcaggctcc ccccgctgct gcggaccctg
     4861 cgccagtccg gcctgcagat ggcggtcgtc gtcgacgagt atggcgggac ctccggcgtc
     4921 gtcacgctcg aggacgtcat cgaggagctc gtcggcgagg tcgacgacga gcacgaccgg
     4981 ccgcactcga ccgggcgtcg gcttaccgac ggccagtggg cggtgccggg cctgtggcgc
     5041 ccggacgagg tgcgcgtccg gatcggcgcc gtgatccccg acggaccgac gtacgagacg
     5101 accggcggct acgtcatggc cgtgctcggt cgcgttcccg ctgtcggtga cgaggtcgac
     5161 ataccggggt ggcgtgtgcg cgttgccgcc atggatggcc ggcgggtgga ccggttgcgg
     5221 ttcatcccgg ccccggagcc ctccggggac gcggcccgcg acgacacgcc ggcccgcggg
     5281 gcgtcgtcgt gagccagacc tcggcgctgc tcgtctcgct gggtcttctc ctcggcaacg
     5341 ccttcttcgt cggggcggag ttcagtgcga tggccgcccg acgatcctcc ctcgaaccgc
     5401 tcgccgagag cggcaaccgc cgcgccgtgg tctgcctcga ggcgctcgag tcgatgggtt
     5461 ccatgctggc gacggcccag ctcggcatca cggtctgctc ggtcgggctg ggggccctgg
     5521 ccgaggtggc cctcgaacag ctcctcgagc ctgtcctgca cagctcgggt cttgcgagcg
     5581 agctggcggc tccgatcgcg ctcgggatct cgctgctgat cgtcgtctac ctgcacgtcg
     5641 tcgtcggcga gatgatcccc aagaacctcg ccctcgccgg cccgggccag tcggcgctgg
     5701 tcctcgtgcc ggccctgctc tacctgagcc gggcgctgcg tcccttcatc cggctcatgg
     5761 agtgggttgc caaggcgctg gtgcgcctct tcggagtcga gcccagggat gagatcacgt
     5821 cggcgttcac ggccgaggag gtcgcccaca tcgtcgtcga gagccatcgg gagggcctca
     5881 tcgaggggga ccggcatggc ctggtccgca ggaccctcga gttcagcgac aaggtcgcac
     5941 gggacgtcgc tgtcccggct tcggccctgg cgacgatcga gctcggcgcg acacccgcgg
     6001 acatcgagcg cctcgtcgcc cgcctcggtt tctcccgctt cccggtcctc gatgccgacg
     6061 gtgacgtcgc cgggtacctc cacgtcaagg acatcctcta cgccgacgac gagcgccata
     6121 ccgagcccgt gccgttgaag cgggttcgcc gtctggccac cgtctcgccc ggcgacgagg
     6181 tcgaggaggt gctcgcgacg at
//
LOCUS       DATAIX010000758         2036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_146977, whole genome shotgun sequence.
ACCESSION   DATAIX010000758 DATAIX010000000
VERSION     DATAIX010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146977"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1772
                     /locus_tag="VHM65_11210"
     CDS             261..1772
                     /locus_tag="VHM65_11210"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HEX2706319.1"
                     /translation="MSIPPFTDHSEGQPDPASPVDNPPRARKRVALIGGGVALALAVG
                     AGAVAFTTLSSKGAQPDTVMPGEVALYARIDLDPSAGQKVNTVRFLSKLPKSKGDLAK
                     DPRQALFSMIKDGSSGLKDIDYAKDVEPWLGDRLAVGVLPGAAGAEPVAVVAVQVKDE
                     AKAKDGIARLMKSGDGTAEVTMKDGYALISEKGTSAAAVTASLAKGSLATNKTYLEDM
                     AALGDPGIASLWADTTPLLQAIPSDLLSTTGGKAPAGTTPDVLKDTGRVAAAWRFDAD
                     YVEVAGIVRGAKAPKVAPKAGLASMTSLPDSTVGAVQVSGAGEGAVSVWEQVRSALDS
                     SGIASEDLGTQLGLTLPADLLTLLGSSLTVAVPEQDFGSDLPTVGLKVDSPDATKAAS
                     LIEGMFASAGSTPLPMSVQGSTYYLSTTESYLDALKSGGTLGSTPAFAAAVPNADTAT
                     AAMYVDLDKIEKTYRDDLSADLKDMVLAMRSVGISGSQDGKGNGTFTMRVVSN"
     gene            1825..>2036
                     /locus_tag="VHM65_11215"
     CDS             1825..>2036
                     /locus_tag="VHM65_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2706320.1"
                     /translation="MPCPVGRTERRAFAASPADILITTPESLFLMLTSGVRAALTGVE
                     TVILDEIHAVAGTKRGAHLAVTLEPQA"
BASE COUNT          325 a          748 c          696 g          267 t
ORIGIN      
        1 gtccgcccac cgaccgcggc gaggacctgg tcgtgagcca gcgacccggc atacccctgc
       61 ccgaactcgt cggacatgag ggtccagaac tggctcaggc gcacgggcgg agtgtgtcac
      121 acccccaggc ggaggtatga cgccccccac cgccgcggcc ccgtgcggag cctcgccggg
      181 gcgccggggc tcgtggcgac gcgtagactg ggctcagcag cggcgctctc gtgccggtgc
      241 aaccgctatc agggggccca atgtccatcc caccgttcac cgaccacagc gaggggcagc
      301 ccgacccggc ttcaccggtg gacaaccccc cgcgcgcccg caagcgggtg gcgctcatcg
      361 gcggcggtgt ggccctggcc ctcgcggtcg gcgcgggcgc cgtggcgttc accacgctca
      421 gctccaaggg cgcgcagccc gacacggtca tgccgggtga ggtcgcgctc tacgcccgga
      481 tcgacctgga cccgtcggcg ggccagaagg tcaacacggt tcgcttcctg agcaagctgc
      541 ccaagtccaa gggtgacctc gccaaggacc cgcgccaggc actgttctcc atgatcaagg
      601 acgggtcgag cgggctcaag gacatcgact acgccaagga cgtcgagccc tggctcggcg
      661 accggctcgc cgtcggagtc ctgcccgggg cagcaggcgc cgagccggtt gccgtcgtcg
      721 cggtccaggt caaggacgag gccaaagcca aggacggcat cgccaggctg atgaagtccg
      781 gcgacgggac ggccgaggtg acgatgaagg acggctacgc cctcatcagc gagaagggca
      841 cctcggcggc ggcagtgacc gcgtccctgg ccaagggcag cctcgcgacc aacaagacct
      901 acctcgagga catggcggcg ctgggcgacc cgggcatcgc gtcactctgg gccgacacga
      961 cgccgctgct ccaggccatc cccagcgacc tgctttcgac cacgggcggg aaggcccccg
     1021 ccgggacgac ccccgacgtg ctcaaggaca cgggacgcgt ggcggcggcc tggcgcttcg
     1081 acgccgacta tgtcgaggtc gcggggatcg tgcgcggagc caaggcgccc aaggtcgcac
     1141 cgaaggctgg gcttgcgtcc atgaccagcc tccccgactc gaccgtcggc gctgtgcagg
     1201 tcagcggtgc cggcgagggg gcggtgtcgg tctgggagca ggtcaggtcc gcgctcgact
     1261 ccagcgggat cgccagcgag gacctgggca cccagctcgg gctcaccttg ccagcagacc
     1321 tgctgacact gctcggcagc tcgttgaccg tcgccgtgcc cgagcaggac ttcggctcgg
     1381 acctgcccac cgtgggcctc aaggtggact cacctgacgc caccaaggct gcgtcgctca
     1441 tcgagggcat gttcgcctcg gccggcagca cgccgctgcc gatgtcggtc cagggctcga
     1501 cgtactacct gagcacgacc gagtcctacc tcgacgccct gaagtccggc ggcacgctcg
     1561 gctcgacgcc ggccttcgcg gccgccgtcc ccaacgccga caccgcaacg gccgcgatgt
     1621 atgtcgacct cgacaagatc gagaagacct accgggacga cctgtccgcc gacctcaagg
     1681 acatggtgtt ggcgatgcgt agtgtcggga ttagcgggtc ccaggacggc aagggcaacg
     1741 ggaccttcac gatgcgggtc gtcagcaact agcgcgccgt ccccgggcaa gggcgccgga
     1801 ggcaccgtcc ctgcgcaccg tgctatgccg tgcccggtcg gccgcaccga gcgacgggcc
     1861 tttgccgcgt cgccggccga catcctcatc acgacgcccg agtcgctctt cctcatgctc
     1921 acctccggcg tccgcgcggc gctgaccggc gtcgagacgg tcatcctcga cgagatccac
     1981 gccgtggccg gcaccaagcg cggggcccac ctcgccgtga ccctggaacc tcaggc
//
LOCUS       DATAIX010000759         3359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_266991, whole genome shotgun sequence.
ACCESSION   DATAIX010000759 DATAIX010000000
VERSION     DATAIX010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266991"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1007)
                     /locus_tag="VHM65_11220"
     CDS             complement(138..1007)
                     /locus_tag="VHM65_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706321.1"
                     /translation="MTRVLALADWQFRARAHEARVDAATQAHLGRRGAGRTHPVEDFL
                     WTYYSLRPGQLRRWHPGPGVRLAEAAGMPRAGWRFYRAVTGGVEVDVDAFVAARRQTL
                     EWVLALLRATQGRSPQLACFGLHEWAMVYRQQPGALRHTGWSLRLGSRATDAVVESHQ
                     VRCTHDGAFRFFTPQARALNIVQPHRDSQIELEQPGCLHANMDLYRWAYRLAPAIPSE
                     LVMACLELARSAREIDMRSAPYDLRDLGYEPIRIETPDGKTEYVTAQRRIADRAQGLR
                     SQLIDACCAVLAQ"
     gene            1090..1890
                     /locus_tag="VHM65_11225"
     CDS             1090..1890
                     /locus_tag="VHM65_11225"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX2706322.1"
                     /translation="MRVARYTTGEDPAYGVIENAGGQLVVARIHGDPLYQPIALTGER
                     IPLDEVRLLAPVIPRSKVIGIGKNYADHAAEMGGAPPAEPMMFLMPNTAIIGPGDPVV
                     MPWQSTEVHYEGELAVVIGKVCRDVPVERALAVVFGYTCGNDVTARDLQRLDGQWSRA
                     KGFDTFCPIGPWIETELDPSALHLQTRRDGEVVQDGTTADMVHDVAALVAYASSAFTL
                     LPGDIIMTGTPAGVGPVRAGQRVEVEIEGIGTLSNPFVQREDRPARLG"
     gene            1898..>3359
                     /gene="gltX"
                     /locus_tag="VHM65_11230"
     CDS             1898..>3359
                     /gene="gltX"
                     /locus_tag="VHM65_11230"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX2706323.1"
                     /translation="MSENTAPVRLHVAPSPTGDPHVGTAYMSLFNLGFVRQRGGRFVL
                     RIEDTDRARFRDDSEGQVFDTLHWLGLTWDEGPDIGGPHAPYRQSERLATYRPYVERL
                     LASGHAYHCWCSSERLEQMRTEQQRRKQPTGYDRLCVGKTPEERAALPGFSEAPVVRM
                     LIPDDVSLTFTDLIRGEVNAPRPDDQVILKADGFPTYHLAVVVDDHEMAITHVVRGEE
                     WISSTPKHVLLYQWLAIPVPQFAHMPLLRNVDKSKISKRRNPAARLTWFRQRGYLPEA
                     LVNFLALMAYPPMQDADGSDREVFTFAEFSEHFDWAKVNPVGPVFDLKKLGWLNGIYI
                     RALPVGELASRLLPYLEQAELLASPPSLGELGRLQALAELVQTRIEVLSDAVPLVTPF
                     YVGDDMIEVAGEARATLDADSARVLDTAIHTLADIDDHASPGVLGAHPSFNAAAIERA
                     LRSAVVDGLGLKARVAFGPLRVALSGQRISPPLFESM"
BASE COUNT          493 a         1219 c         1164 g          483 t
ORIGIN      
        1 gcctggggcc ggccagatcg gcctcgatcc cagcggggcc agcaccgagg tggttccggc
       61 cgcctggggc cggccagatc ggcctcgatc ccagcggggc cagcaccgag gtggttccgg
      121 ccgccagtgt ccgtgtgtca ctgagccagg acggcgcagc aggcgtcgat gagctgcgac
      181 cgaaggccct gggcgcggtc ggcgatccgg cgttgcgcgg tgacgtactc ggtcttgccg
      241 tcgggggtct cgatgcggat cggctcgtag ccgaggtcgc gcaggtcgta gggcgccgac
      301 cgcatgtcga tctcgcgggc ggaccgggcc agctcgaggc aggccatgac gagctcgctc
      361 gggatcgcgg gcgccagccg gtaggcccac cggtagaggt ccatgttcgc gtggaggcac
      421 ccgggctgct cgagctcgat ctgggagtcg cggtggggct ggacgatatt gagcgcgcgc
      481 gcctgcgggg tgaagaacct gaacgcgccg tcgtgcgtgc acctcacctg atgcgactcg
      541 accaccgcgt cggtggcccg cgagccaaga cgcaacgacc atccggtatg ccggagcgcc
      601 cccggctgct gccggtagac catcgcccac tcatgcaggc cgaagcaggc cagctggggc
      661 gatcgaccct gggtagcccg cagcagggcc agcacccact cgagcgtctg gcgccgggcg
      721 gcgacgaacg cgtcgacgtc gacctcgacc ccgcccgtca ctgcccggta gaagcgccag
      781 ccggcccgcg gcataccggc tgcttcggcc agccggacgc cgggaccggg atgccagcgg
      841 cggagctgcc cgggccgcaa cgagtagtag gtccacagga agtcctcgac cggatgcgtg
      901 cggccggctc cgcgtcgccc cagatgggcc tgcgtcgccg catcgacgcg cgcctcgtgg
      961 gcgcgcgccc ggaactgcca gtcggcgaga gcgagcacgc gggtcacgag ggaaggctac
     1021 gacccggcca gctcgaccgc gcggcgccag ggcccacgac cctctggcgg cggtcgtagg
     1081 ctggggacca tgcgggtcgc gaggtatacg acgggggagg acccggcata cggggtcatc
     1141 gagaacgccg gcggacagct ggtggtcgcg cggatccatg gggacccgct ctaccagccg
     1201 atcgccttga cgggcgagcg catcccgctc gacgaggtgc ggctgctcgc gccggtcatc
     1261 ccgcgcagca aggtgatcgg catcggcaag aactatgccg accacgccgc cgagatgggt
     1321 ggcgccccgc cggccgagcc gatgatgttc ctcatgccca acaccgcgat catcggcccc
     1381 ggcgacccgg tcgtcatgcc ctggcagagc accgaggtcc actacgaggg tgagctcgcc
     1441 gtcgtcatcg gcaaggtgtg tcgcgacgtc ccggtcgagc gggcgcttgc cgtggtgttc
     1501 ggctacacgt gtggcaacga cgtcaccgcc cgcgatctgc agcgtctcga tggccagtgg
     1561 tcgcgggcca agggcttcga cacgttctgc ccgatcgggc cgtggatcga gaccgagctc
     1621 gacccgagcg ccctgcacct gcagactcgc cgcgacggcg aggtcgtcca ggacgggacg
     1681 acggccgaca tggtccacga cgtggcggcc ctggtcgcct acgcttcgtc ggccttcacc
     1741 ctgctgcccg gcgacatcat catgaccggc acccctgccg gggtcggccc ggtccgggcg
     1801 ggccagcgcg tcgaggtcga gatcgagggc atcggcaccc tgtccaaccc cttcgtccag
     1861 cgggaggacc gccctgcccg gctaggctga gcgaactatg agcgagaaca cggcccctgt
     1921 ccggctccac gtggccccct cgccaaccgg tgacccgcac gtcggcacgg catacatgtc
     1981 gctgttcaac ctcggtttcg tgcgccagcg cggtggccgg ttcgtgctcc gcatcgagga
     2041 caccgaccgg gctcgcttcc gcgacgacag cgaggggcag gtcttcgaca cgctgcactg
     2101 gctgggcctg acctgggacg agggaccgga catcgggggc ccgcacgcgc cataccggca
     2161 gtccgaacgc ctggcgacct accgcccgta tgtcgagcgc ctcctcgcgt ccggccacgc
     2221 ctaccactgc tggtgctcga gcgagcggct cgagcagatg cgcacggagc agcagaggcg
     2281 caagcagccg accgggtacg accggctctg cgtcggcaag acccctgagg agcgagcggc
     2341 gctgcccggc ttcagcgagg caccggtcgt gcggatgctt atccccgacg acgtgtcact
     2401 gaccttcacc gacctgatcc gcggcgaggt caacgccccg cgcccggacg accaggtgat
     2461 cctcaaggcc gacggcttcc cgacctacca ccttgcggtc gtcgtcgacg accacgagat
     2521 ggcgatcacc cacgtcgtgc gcggcgagga gtggatctcc tcgacgccca agcacgtgct
     2581 cctctaccag tggctcgcca tccctgtccc gcagttcgcc cacatgccac tgctccgcaa
     2641 cgtcgacaag tccaagatct ccaagcgcag gaacccggcg gctcgcctga cctggttccg
     2701 gcagcggggc tacctgcccg aggcgctcgt caacttcctt gccctgatgg cctatccgcc
     2761 catgcaggac gcggacggct cggaccggga ggtcttcacc ttcgcggagt tctccgagca
     2821 cttcgactgg gccaaggtca acccggtggg tcccgtcttc gacctcaaga agctcggctg
     2881 gctcaacggc atctacatcc gggccctgcc ggtgggcgag ctggcgtcgc ggttgctgcc
     2941 ctacctggag caggccgagc tgcttgcgag cccgccgagc ctgggcgagc tcggccggct
     3001 ccaggcgctc gccgagctcg tccagacccg catcgaggtg ctctccgacg ccgtgccgct
     3061 cgtcacgccc ttctatgtcg gcgacgacat gatcgaggtg gccggcgagg cgcgcgcgac
     3121 gctcgacgcc gactcggccc gggttctcga caccgcgatc cacaccctcg ccgacattga
     3181 cgaccacgcc tcgcccggtg tcctcggcgc gcacccctcg ttcaacgccg cggcgatcga
     3241 gcgcgcgctg cggtcggccg tcgtcgacgg gctcgggctc aaggcccggg tggctttcgg
     3301 tccgctgcgg gtcgccctct cgggtcagcg gatctcgcca ccgctgttcg agtcgatgc
//
LOCUS       DATAIX010000760         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_147082, whole genome shotgun sequence.
ACCESSION   DATAIX010000760 DATAIX010000000
VERSION     DATAIX010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147082"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..538
                     /locus_tag="VHM65_11235"
     CDS             29..538
                     /locus_tag="VHM65_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706324.1"
                     /translation="MALTADRVTDLMNDCTEFEFVDTFLQSLDDSHGLLVRTVVVARD
                     EIFAVGVAGPRGNPTRRTRTRPVPVELQLGRYDVSGNIHVVPGTDPIIGFRRRRVMVP
                     LTEATIEFDSPDGRKLARFGTILVNRDLTDWIAPASRSDVRPPDVVPELEGRGLAKDF
                     TPSLLVHEA"
     gene            complement(754..1413)
                     /locus_tag="VHM65_11240"
     CDS             complement(754..1413)
                     /locus_tag="VHM65_11240"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX2706325.1"
                     /translation="MGETIRIQAAAGDTDTYLAPARPSPGAPVLLLHPWWGLNQTIRD
                     LADRLAGDGFTVMAPDLFDGTVLTTPDDAKAHVTSLTQADGDRIRARVQATLDHLLAH
                     PDARGDRAAIIGMSFGAMEGTEVATARPDVAALVTFYSGIFEAPEGIAYLGHFAEDDE
                     FDDSAQVPDLQRTLGEGSAAHVYPGTKHWFIEADRPEFDADATELAYSRTVAFLREQL
                     A"
     gene            complement(1524..>2292)
                     /gene="msrA"
                     /locus_tag="VHM65_11245"
     CDS             complement(1524..>2292)
                     /gene="msrA"
                     /locus_tag="VHM65_11245"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX2706326.1"
                     /translation="ATGGRCGRHLPVTSLAVRSALTSRNPEIRMGILDFLFAAPAKSQ
                     IPSPADALPGRPEPILAPGRHTVLGTPIDGPWPAGTRTVTFGMGCFWGAEKGFWAIPG
                     VVSTAVGYAGGSTPNPTYQEVCTGRTGHAEVVRAAYDPARVSFDTLLRAFWEAHDPTQ
                     GMRQGNDTGTQYRSAIYVADSDELVLAEASRDAYQERLAAAGFGQITTEIAPAGPFFF
                     AEDYHQQYLDKNPNGYCPDHGTGVACPIGLGMKAGAT"
BASE COUNT          326 a          759 c          837 g          370 t
ORIGIN      
        1 cgcggatgac tccgtggcat tcggccgcgt ggcgctcacc gcggaccggg tgaccgacct
       61 catgaacgac tgcaccgagt ttgagttcgt cgatacgttc ctgcagagcc ttgacgacag
      121 ccacgggctg ctggtcagga cggtcgtcgt cgcgcgagac gagatcttcg ccgtgggggt
      181 ggcgggcccg cgtggcaatc caacgcgtcg gacccggacc cgcccggtcc ccgtcgagct
      241 tcagcttgga cgctacgacg tcagcgggaa catccacgtg gtgcccggca ccgacccgat
      301 catcggcttc cgtcgtcgac gagtcatggt tccgctcacg gaggcgacga tcgagttcga
      361 ctcgccggac ggacggaagc tcgcccggtt cggcaccatc ctcgtgaacc gggatctcac
      421 tgattggatc gcacccgcat cgcggtcgga cgttcggccg cccgacgtgg tcccggagct
      481 cgaaggtcgc ggcctcgcga aggacttcac tccctcgctg ttggttcacg aggcgtaagt
      541 cgggcgcgcg gtcgccccgc cagactcctg ccgccggcta ttcggcgggc accccgcgca
      601 gcccgatcgg gcaggccacc ccggtcccgt ggtccggaca gtagccgttc gggttctggg
      661 ataggtagtg atcggtcggc gcctactctg gagggcaacg cgtcaagatg cctcagccgc
      721 gccggagtcg cgtcgcagcc gccctgaccg accctaggcg agctgctcgc gcaggaaggc
      781 gacggtgcgc gagtaggcga gctcggtcgc gtcggcgtcg aactctggac ggtccgcctc
      841 gatgaaccaa tgcttcgtgc cggggtagac gtgcgcggcg ctgccttcgc cgagcgtccg
      901 ctgcaggtcg ggcacctggg cggagtcgtc gaactcgtcg tcctctgcga aatggccgag
      961 gtaggcgatc ccctcgggcg cttcgaagat gccgctgtag aaggtgacga gggcggcgac
     1021 gtcgggtcgc gcggtggcga cctcggtgcc ttccatggcg ccgaagctca tgccgatgat
     1081 cgcggcacgg tcaccacgag cgtcggggtg ggccaggaga tggtcgagcg tggcctggac
     1141 gcgggccctg atgcggtcgc cgtcggcctg tgtgagcgag gtgacgtgcg ccttggcgtc
     1201 gtccggcgtg gtcaggacgg tgccatcgaa caggtccggc gccatgaccg tgaagccgtc
     1261 gccggcgagc cggtcggcca ggtcgcggat cgtctggttc aggccccacc acgggtggag
     1321 cagcagtacc ggcgcgcccg gtgacggtcg tgccggcgcg aggtaggtat cggtgtctcc
     1381 agccgccgcc tggatgcgga tcgtttctcc catgcgggga aggatactag tggcaaggcc
     1441 ggtcatgagt ccttcggctc aggccgattg cctgcaggac gatgccgatg aactgcagcg
     1501 ccaagccgag gctcatcggg tggctaggtg gcgcccgcct tcatcccgag cccgatcggg
     1561 cacgcgaccc cggtcccgtg gtccggacag tagccgttcg ggttcttgtc caggtactgc
     1621 tggtggtagt cctcggcgaa gaagaacggt ccggcgggcg cgatctccgt cgtgatctgg
     1681 ccgaaccccg ccgccgccag gcgttcctgg tacgcgtccc gcgatgcttc ggcgagcacc
     1741 agctcgtcgg agtcggcgac gtagatggcg gatcggtact gggtgcccgt gtcgttgccc
     1801 tggcgcattc cctgcgtcgg gtcatgcgcc tcccagaacg cgcgcagcag ggtgtcgaac
     1861 gagacgcgcg ccgggtcgta ggccgcgcgg accacttccg catgcccggt tcggccggtg
     1921 cagacttcct ggtaggtcgg gttcggcgtg ctgccaccgg cgtaccccac cgcggtgctc
     1981 accacgccgg ggatcgccca gaagcccttc tcggcgcccc agaagcagcc cataccgaag
     2041 gtcacggtcc gcgtaccggc gggccagggt ccatcgatcg gcgtgccgag gaccgtgtgg
     2101 cgccccggcg ccaggatcgg ctcggggcgg ccgggcagcg cgtcagcggg ggaggggatc
     2161 tgcgacttgg caggggcggc gaacaggaag tcgagaatgc ccatgcggat ctccgggttg
     2221 cgggacgtca gggcggatcg taccgcgagt gacgtgacgg gcagatgacg cccgcaccgc
     2281 ccgcccgtcg cg
//
LOCUS       DATAIX010000761         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_267054, whole genome shotgun sequence.
ACCESSION   DATAIX010000761 DATAIX010000000
VERSION     DATAIX010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267054"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VHM65_11250"
     CDS             complement(<1..188)
                     /locus_tag="VHM65_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706327.1"
                     /translation="MITATRPSTSSASASIAAETAPAGDSVKVVSAGRGPDGLVLVAL
                     DRRDDVAHPGAAAAGQGG"
     gene            181..405
                     /locus_tag="VHM65_11255"
     CDS             181..405
                     /locus_tag="VHM65_11255"
                     /inference="COORDINATES: protein motif:HMM:NF024928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HEX2706328.1"
                     /translation="MITPARHRALLDRFDGSAQGRVTVVDPMSAKGLEYDATVIVDPD
                     AITAESPGGVRVLYVALTRAAHRMTVVRPA"
     gene            complement(415..1704)
                     /locus_tag="VHM65_11260"
     CDS             complement(415..1704)
                     /locus_tag="VHM65_11260"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX2706329.1"
                     /translation="MPAVVLVGAQWGDEGKGKATDLLGSSVDYVVKFNGGNNAGHTIV
                     IDGETYALHLLPSGILTPGCTPVIGNGVVVDLGVLFEELDALDARGVDTSRLLVSANA
                     HVIAPYNRVLDRVTERFLGARKLGTTGRGIGPTYADKMSRTGIRVQDLFDEHILRQKV
                     EAALAFKNQVFAKIYNHRAIDADEVVSELLSYVDRLRPMVTDTGLVLSEALDAGRTVL
                     LEAGQATLLDVDHGTYPFVTSSNATSGGACTGSGIPPTRVDRVIAVLKAYTTRVGEGP
                     FPSELDDELGEHLRKAGAEFGTTTGRPRRCGWLDTVVGRYATRVNGVTDFVVTKLDVL
                     TGLAEVPICVAYDVDGVRHDTMPVNQSDFHHAKPIYEVMPGWWEDITACRSFEELPAT
                     AQAYLLRVEDLIGARISAIGVGPSREAIIERYPLLGP"
BASE COUNT          248 a          651 c          643 g          278 t
ORIGIN      
        1 tggccgccct gacccgcagc cgcggcgccc ggatgcgcga catcgtcgcg acgatccagc
       61 gccaccagga cgaggccatc cgggcccctg cccgcggaga cgaccttcac cgagtccccc
      121 gcaggagcgg tctcggccgc gatcgacgcg ctggccgacg aggtcgacgg tctcgtcgcg
      181 gtgatcaccc cggcgcgcca tcgggccctc ctcgaccggt tcgacggctc ggcgcagggc
      241 cgggtcaccg tcgtcgaccc gatgtcggcc aagggcctgg agtacgacgc caccgtcatc
      301 gtcgaccctg acgccatcac cgccgagtcc cccggtgggg tccgcgtgct ctacgtcgcg
      361 ttgacccggg cggcccaccg gatgaccgtg gtccgcccgg cctgacggcg gccctcaggg
      421 gccgagcagc gggtagcgct cgatgatcgc ctcccgcgag gggcccacgc cgatcgccga
      481 gatccgggca ccgatcaggt cctcgacccg cagcaggtag gcctgggcgg tggccggcag
      541 ctcctcgaag ctgcggcacg ccgtgatgtc ctcccaccag ccgggcatga cctcgtagat
      601 cggcttggcg tgatggaagt cgctctggtt gaccggcatc gtgtcgtggc gaaccccgtc
      661 cacgtcgtag gccacgcaga tcgggacctc cgcgagcccg gtgagcacgt cgagcttggt
      721 cacgacgaag tcggtgacgc cgttgacccg ggtggcgtac cgtcccacga ccgtgtccag
      781 ccagccgcac cggcgcggcc gcccggtcgt cgtgccgaac tcggcaccgg ccttgcgcag
      841 gtgctcgccg agctcgtcgt ccagctcgct cgggaacggc ccctccccca ctcgggtcgt
      901 gtaggccttg agcaccgcga tgacccggtc cacccgcgtc ggcgggatcc cggaccccgt
      961 gcaggcccca cccgacgtcg cgttcgacga cgtgacgaag gggtaggtcc cgtggtcgac
     1021 gtcgagcagg gtggcctgac cggcctcgag gaggacggtc cggccggcgt cgagtgcctc
     1081 actgagcacg agcccggtgt cggtgaccat gggacgcaac cggtcgacat acgacaggag
     1141 ctcgctgacc acctcgtcgg cgtcgatcgc gcggtggttg tagatcttgg cgaacacctg
     1201 gttcttgaag gcgagcgcgg cctcgacctt ctggcgcagg atgtgctcgt cgaacaggtc
     1261 ctggacccgg atccccgtgc ggctcatctt gtcggcatag gtcgggccga tgccccgccc
     1321 ggtcgtcccg agcttgcggg cgccgaggaa gcgctcggtc acccggtcca ggacccggtt
     1381 gtacggggcg atgacgtgcg cgttcgcgga cacgagcaga cgcgaggtgt cgacgccgcg
     1441 cgcgtccagg gcgtcgagct cctcgaagag cacgccgagg tcgacgacga cgccattgcc
     1501 gatgaccggc gtgcagcccg gcgtgaggat ccccgagggg agcaggtgca gggcgtacgt
     1561 ctcgccgtcg atgacgatcg tgtgcccggc gttgttgcca ccgttgaact tgacgacgta
     1621 gtcgacgctg ctcccgagca gatcggtggc cttgcccttg ccctcgtcgc cccattgggc
     1681 gcccaccagc acgactgcag gcatcgggct gtccctccgt gtgctcggtc gtgccgtggt
     1741 cggtcgtgcg cggtggtcgg tcgtgcgcgg tggtcagaag cccagctcac gtgctgcggt
     1801 ggccgagctg gcccgcagga
//
LOCUS       DATAIX010000762         1889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_267113, whole genome shotgun sequence.
ACCESSION   DATAIX010000762 DATAIX010000000
VERSION     DATAIX010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267113"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VHM65_11265"
     CDS             <1..926
                     /locus_tag="VHM65_11265"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX2706330.1"
                     /translation="PQGVRKATLELLTLARRLGEPAAVLCGAADDAVVGALGEYGASV
                     VYVVPGTEEFLSVPAAEALVEIAGRVQPAAILITSGPTGKDVAARAAVRLDSGIITDA
                     VDVQAGDGGLTATQSVFAGTWHVSSQVVRGVPVVTVRPNAISAEPAPVTPRVEQVEVG
                     FSDTARGAKITSRSPKVSSGRPELTDAGVVVSGGRGVGTQEGFEVIERLADALGAAVG
                     ATRAVTDLHWVAHDLQVGQTGKTVAPSLYVAAGISGAIQHRAGMQSSKTIVAVNKDPK
                     APIFGVSDFGVVGDLHKVVPALIDEIAKRKH"
     gene            985..>1889
                     /locus_tag="VHM65_11270"
     CDS             985..>1889
                     /locus_tag="VHM65_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706331.1"
                     /translation="MLVVRLVLGLLMTFVCLGIAGRRLFFLYRLAKAGQPVPAGRVSG
                     AGETVKAELVEVAGQRKLLRWTGPGLAHFAVFWGFMVLVLTIIEGFGSLVWPTFHIPV
                     VGNYAWLGFFEDLFALLCIGAVLAFVAIRVREAPKDKGRASRFFGSHLGPAWFTLFMI
                     FNVVWTLVFARAAQINAQEVNTATDGRLGFLKGAFAAQWIAGLLEPLGRHTNEILETV
                     LLLLALGVLLGFTVFVTYSKHLHIFLSIPNVAFARRPRALGALLPLYSGGTKVDFEDP
                     GDDDLLGVGTIDGFTWKGLLDFGTCT"
BASE COUNT          247 a          704 c          626 g          312 t
ORIGIN      
        1 ccccacaggg tgtccgcaag gccaccctcg agctgctcac cctcgcccgc cgcctcggcg
       61 agcccgccgc cgtcctgtgc ggcgccgcgg acgacgccgt ggtcggtgcg ctgggcgagt
      121 atggcgcgtc cgtcgtctac gtcgtgcccg gcaccgagga gttcctctcg gtccccgcgg
      181 ccgaggcgct cgtcgagatt gccggccggg tccagcccgc ggcgatcctc atcacctccg
      241 gcccgacggg caaggacgtc gccgcccggg ccgcggtccg gctcgactcc gggatcatca
      301 ccgacgcggt cgacgtccag gccggcgacg gtggcctcac cgcgacgcag tccgtcttcg
      361 ccgggacgtg gcacgtgtcg agccaggtgg tgcgcggcgt ccccgtcgtc accgtgcgcc
      421 ccaacgcgat cagcgcggag cccgcccccg tgacgcctcg ggtcgagcag gtcgaggtcg
      481 gcttcagcga caccgcgcgc ggggccaaga tcacctcgcg ctcgcccaag gtgtcctcgg
      541 gccgtcccga gctcaccgac gccggcgtcg tcgtctccgg tggccgcggg gtcggaacgc
      601 aggagggctt cgaggtcatc gagcgccttg ccgacgccct cggtgcggcc gtcggcgcca
      661 cccgcgccgt caccgacctg cactgggtcg cgcacgacct gcaggtcggc cagaccggca
      721 agaccgtcgc cccctcgctg tatgtcgcag ccggcatctc cggagcgatc cagcaccgcg
      781 ccggcatgca gagcagcaag acgatcgtcg cggtcaacaa ggaccccaag gcgccgatct
      841 tcggcgtctc ggacttcggt gtcgtcggcg acctgcacaa ggtcgtcccg gcactcatcg
      901 acgagatcgc caagcgcaag cactgaccgc actaaccgca ctgaccgcac tgacccgagc
      961 acggggggag aaggagtccg ctagatgttg gtcgttcggc tcgtcctggg cctgctcatg
     1021 acgttcgtct gcctcgggat cgccggacgg cggctcttct tcctctaccg cctcgccaaa
     1081 gccgggcagc ccgttcccgc gggccgggtg tcgggggccg gtgagacggt caaggccgag
     1141 ctcgtcgagg tggccgggca gcgcaagctg ctgaggtgga ccgggcccgg cctggctcac
     1201 ttcgcggtgt tctggggctt catggtcctc gtcctgacga tcatcgaggg cttcgggtcc
     1261 cttgtctggc ccaccttcca catcccggtc gtcggcaact acgcgtggct cggcttcttc
     1321 gaggacctct tcgcgctgct gtgcatcggc gcggtgctcg cgttcgtcgc gatccgggtc
     1381 agggaggccc ccaaggacaa gggccgggcc tcacgcttct tcgggtcgca cctcggcccg
     1441 gcgtggttca ccctcttcat gatcttcaac gtcgtgtgga ccctggtctt cgcccgcgcc
     1501 gcccagatca acgcccagga ggtcaacacc gccaccgacg ggcggctcgg gttcctcaag
     1561 ggagccttcg ccgcgcagtg gatcgccggg ctgctcgagc cgctgggccg tcataccaac
     1621 gagattctcg agaccgtcct gctgctcctc gcgctcggtg tcctgctcgg gttcacggtc
     1681 ttcgtgacct acagcaagca cctgcacatc ttcttgtcga tccccaacgt cgccttcgcg
     1741 cgtcggccac gggcgctcgg cgcgctgctg cccctctact ccggcgggac gaaggtcgac
     1801 ttcgaggacc cgggcgacga cgacctcctc ggtgtcggca cgatcgacgg cttcacctgg
     1861 aaagggctgc tcgacttcgg cacgtgcac
//
LOCUS       DATAIX010000763         3959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_147148, whole genome shotgun sequence.
ACCESSION   DATAIX010000763 DATAIX010000000
VERSION     DATAIX010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147148"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VHM65_11275"
     CDS             complement(<1..769)
                     /locus_tag="VHM65_11275"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2706332.1"
                     /translation="MTFSWTYALVALALVPAALAAYLWLLRRRRRTAIRFSNVALVRA
                     ALPARSRWRRHVPFALVLAAVGLLALGSARPQVSVQVPMQRTSVILAIDVSRSMCAVD
                     VEPNRLSAAQEAVRGFIQDQDDDTRIGLVAFAGYAELVVAPTVDRQSLVDAVDALTTG
                     RGTTIGAAILKAVDAIAAINPDVAPVGPTEADGETGGDDRGWGSGPAPSPSAGPVPAP
                     SPTPAREPVPDIVVLLTDGANTRGITPVAAARVAATRG"
     gene            complement(766..1758)
                     /locus_tag="VHM65_11280"
     CDS             complement(766..1758)
                     /locus_tag="VHM65_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2706333.1"
                     /translation="MTFALPWLLLGLLALPPLVVAYRRLLRRRERRHTELASLGLVTS
                     APARPARARHLAPLLLLGALTLLLLSLARPAATVADLRREGTVILAFDVSTSMRADDL
                     APTRIDAAKAAARAFVQEQPPSVRIGVVAFNDSGVITQAPTTVQADVVAAIDRMTPAG
                     GTSLGQGIFSSLKAAAGGKLTLGEAALDGDLEDADIGYFGSSVVVLLSDGENTAALDP
                     LKLAELASVAGVRIDTVGIGSADGAVVKVDGMSLATTLDEAMLTSIAEATDGTYYPAA
                     DETTLAQIYETIDLELTSTPRPTEVTALFTLAALALLLASAAVSLRRTGRVMGV"
     gene            complement(1755..2648)
                     /locus_tag="VHM65_11285"
     CDS             complement(1755..2648)
                     /locus_tag="VHM65_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX2706334.1"
                     /translation="MDPTTGLTPERVLRRLEWHVLRRLDGRLQGDYRTLLRGIGVDFL
                     DLREYEPGDDMRHIDWNVTARMDTPYVRQYVEDRELTAWLLLDRSPSMGFGPVTRRKD
                     AVLTELAGTLAALLVRGGNRVGAILYDNRVELTLPPRQGRAQVLLLLRELLREPTPSG
                     TPTDLTGLLTTANGMLRHRSLVVLVSDFISLPGWERPLTQLARRHDVVAIALAAPSES
                     ELPEAGVIVIEDAETGEQLLVDTSDVEFRLRMRDLAASYQDQLDQVARRAGVDLHTLS
                     TGEDLVGALVRMSATRARRHR"
     gene            complement(2627..3658)
                     /locus_tag="VHM65_11290"
     CDS             complement(2627..3658)
                     /locus_tag="VHM65_11290"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX2706335.1"
                     /translation="MNAPPTPSQLLEQTLYEVKRTIVGQDVLLERMVVALLARGHLLV
                     EGVPGLAKTMAVKTLAQALGGEFKRIQFTPDLVPADLIGTRIYNQATGAFQVSLGPVF
                     ANLVLADEINRAPAKVQSALLEVMQERQVTIGRETFRAPDPFLVMATQNPIESEGTYP
                     LPEAQVDRFMLKVLIGYPTPAEEYVVVERQIGPPSTAQPVLTTERLLDLQRAADAVYV
                     DPGIISYAVRVARATRDATSVGLPDLTRYIAFGASPRASINMVLAARALAFVRGRDYA
                     LPQDVHDLALDVLRHRLVLTYEALGDNVEADEILRAVLHAIPLPDVATRPRAPLAPPV
                     EALWTRPPD"
     gene            complement(3711..>3959)
                     /locus_tag="VHM65_11295"
     CDS             complement(3711..>3959)
                     /locus_tag="VHM65_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX2706336.1"
                     /translation="AGVVSATDRSIGLSKDRTLEHLIQFDAAVNPGSSGGPLLDRDGH
                     VVGIVTALANPSRQSFFVGIGFAVPIGTAGGAAGAPAQ"
BASE COUNT          567 a         1310 c         1542 g          540 t
ORIGIN      
        1 cacctcgggt ggccgccacg cgcgcagccg caaccggggt gatgccgcgc gtgttcgcgc
       61 cgtcggtcaa caggacgacg atgtccggca cgggttcgcg ggccggcgtg ggagagggtg
      121 ccgggaccgg gccggccgac ggggacggtg ccggtccgct gccccatccc cggtcgtcgc
      181 cgccggtctc gccgtcggcc tcggtggggc cgacgggggc gacgtcgggg ttgatggcag
      241 cgatcgcgtc gaccgccttg aggatcgcgg ccccgatcgt cgtgccgcgc ccagtcgtca
      301 gcgcgtcgac cgcgtcgacc aggctttgcc ggtccaccgt gggagcgacg actagctcgg
      361 catagcccgc gaaggccacg aggccgatcc gggtgtcgtc gtcctggtcc tggatgaagc
      421 cgcggacggc ctcctgagcg gcgctgagcc ggttcggctc gacgtcgacc gcgcacatcg
      481 acctggacac gtcgatcgcg aggatgaccg aggtccgctg catcggcacc tggacactga
      541 cctgaggccg agccgagccg agcgccagca ggccgacggc cgccaggacg agcgcgaacg
      601 gcacgtgccg gcgccagcgc gagcgggccg gcagggccgc gcgcacgagc gcgacgttgg
      661 agaagcggat cgccgtcctg cgccgccgcc gcagcagcca caggtatgcc gcgagcgcgg
      721 cgggcacgag cgccagggcc acgagggcat aggtccagga gaacgtcata cgcccatcac
      781 ccgcccggtc cggcgcagcg acacggccgc actcgcgagg agcagggcga gcgcagccaa
      841 ggtgaacagg gccgtgacct cggtcggacg gggggtgcta gtcagctcca ggtcgatcgt
      901 ctcgtagatc tgagcgaggg tcgtctcgtc ggcagccggg tagtaggtgc cgtcggtcgc
      961 ctcggcgatg ctcgtgagca ttgcttcgtc gagggtcgtc gcgagactca tcccgtcgac
     1021 cttgacgacc gccccatccg cgctgccgat gcccacggtg tcgatccgca cgcccgcgac
     1081 cgaggccagc tcggcgagct tgagcgggtc cagcgccgcc gtgttctccc cgtcggagag
     1141 caggacgacg accgaggagc cgaagtagcc gatgtcggca tcctccaggt ccccgtcgag
     1201 ggccgcctct ccgagggtca gcttgccccc cgcagcggcc ttgagcgagc tgaagatccc
     1261 ctgtccgagc gacgtgccac ccgccggagt catccggtcg atagcggcca cgacgtctgc
     1321 ctgcaccgtc gtcggtgcct gggtgatgac cccgctgtcg ttgaacgcga cgaccccgat
     1381 ccgcaccgac ggcggctgct cctgcacgaa cgcccgggcc gccgccttgg ccgcgtcgat
     1441 ccgggtcggc gcgaggtcgt cggcgcgcat gctcgtcgac acgtcgaagg ccaggatcac
     1501 cgtcccctcg cggcgcaggt cggcgaccgt ggccgcgggc cgggcgaggg agaggagcag
     1561 caacgtgagc gccccgagga gcaggagcgg ggcgaggtgg cgcgcgcgcg ccggccgggc
     1621 gggcgcggag gtcacgagcc cgagcgacgc gagctcggta tggcgtcgct cccgcctgcg
     1681 cagcagccgg cggtaggcca cgacgagcgg cgggagcgcg agcaggccga gcaggagcca
     1741 gggcagggcg aaggtcatcg gtgccgcctc gctcgcgtgg cggacatccg gacgagggct
     1801 ccgacgaggt cctcccccgt ggacagcgtg tgcaggtcga cgccggcccg gcgggcgacc
     1861 tggtcgagct ggtcctggta agacgcggcg aggtcgcgca tccgcaggcg aaactccacg
     1921 tcactggtgt cgacgaggag ctgctcaccg gtctcggcgt cctctatgac gatgacgccg
     1981 gcctcgggca gctcggactc gctgggggcg gcgagcgcga tggcgacgac gtcgtggcgc
     2041 cgggcgagct gggtcagcgg ccgctcccac ccgggcaggc tgatgaagtc ggagacgagc
     2101 acgacgagcg agcggtgccg aagcatgccg ttcgccgtgg tgagcagccc ggtgaggtcg
     2161 gtcggcgtgc cggacggggt gggctcgcgc aggagctcgc gcaggagcag cagcacctgg
     2221 gcccgcccct gccgcggcgg cagggtcagc tcgacccggt tgtcgtagag gatcgccccg
     2281 acccggttgc cgccacgcac gagcagggcg gcaagcgtcc cggcgagctc ggtgagcacg
     2341 gcgtccttgc gccgggtcac cgggccgaaa cccatcgacg gtgaacggtc gagcagcagc
     2401 cacgcggtga gctcgcggtc ctcgacatac tggcgcacat agggggtgtc catgcgcgcc
     2461 gtgacgttcc agtcgatgtg gcgcatgtcg tcgccgggct cgtactcgcg caggtcgagg
     2521 aagtccacgc cgatgccgcg cagcagcgtg cggtagtcgc cctgcaggcg cccgtcgagg
     2581 cggcgcagga cgtgccattc gagccggcgc aggacgcgct cgggcgtcag tccggtggtc
     2641 gggtccacag agcctcgacc ggtggtgcta ggggcgcccg cgggcgcgtc gcgacatcag
     2701 gcaggggtat ggcgtgcagc accgctcgca ggatctcgtc ggcctcgacg ttgtccccga
     2761 gcgcttcgta ggtgaggacg agccggtggc gcaggacgtc gagcgcgagg tcgtggacgt
     2821 cctgcgggag ggcgtagtca cggccgcgga cgaaggcgag agcacgggcc gcgagcacca
     2881 tgttgatcga ggctcggggg cttgcgccga aggcgatgta gcgggtcagg tcgggcaatc
     2941 cgaccgaggt ggcgtcccgg gtcgcccggg cgacgcggac ggcatacgag atgattccgg
     3001 ggtcgacgta gacggcgtcg gccgcccgct gcaggtcgag gaggcgctcg gtggtgagca
     3061 ccggctgggc ggtgctcggc ggaccgatct ggcgctcgac gacgacgtac tcctcggcgg
     3121 gagtggggta tccgatgagg accttgagca tgaaccggtc gacctgggcc tccgggaggg
     3181 ggtaggtacc ttcggactcg atggggttct gcgtcgccat gacgaggaag gggtcggggg
     3241 cgcggaaggt ctcgcggccg atcgtgacct ggcgctcctg catgacttcg agcagggcgc
     3301 tctggacctt ggcgggggcc cggttgatct cgtcggcgag gacgaggttg gcgaagaccg
     3361 gtccgaggga gacctggaag gcgccggtcg cctggttgta gatccgggtc ccgatgaggt
     3421 cggccggcac gaggtcaggg gtgaactgga tccgcttgaa ctccccgccg agcgcctggg
     3481 cgagcgtctt gaccgccatc gtcttggcca ggccggggac accctcgacg agcaggtgcc
     3541 cccgagcgag gagggcgacg accatgcgtt cgagcaggac gtcctggccg acgatcgtcc
     3601 gcttcacctc gtagagcgtc tgctcgagca gctggctcgg ggtcggtggg gcgttcatgg
     3661 ctctcctggt cattcgcgtc actggtcatt cgcgtcactg gtcattcgtg tcactgggcc
     3721 ggggcgccgg cggccccgcc ggccgtcccg atggggacgg cgaaaccgat gccgacgaag
     3781 aaggactgcc tggaggggtt ggccagcgcc gtgacgatcc cgacgacatg cccgtcgcgg
     3841 tcgagcagcg gcccgccgga gctgcccggg ttgacggcgg cgtcgaactg gatgaggtgc
     3901 tcgagggtcc ggtccttgct gagcccgatc gagcggtccg tcgccgagac gactccggc
//
LOCUS       DATAIX010000764         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_267247, whole genome shotgun sequence.
ACCESSION   DATAIX010000764 DATAIX010000000
VERSION     DATAIX010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267247"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VHM65_11300"
     CDS             <1..227
                     /locus_tag="VHM65_11300"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX2706337.1"
                     /translation="VQRSDDAGELAATALGFTAAMVVVWVFLRFASRIRRVLRESGTV
                     LATRIAGLLLSAIAVQMVADSVRAFVTGAA"
     gene            237..1121
                     /locus_tag="VHM65_11305"
     CDS             237..1121
                     /locus_tag="VHM65_11305"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX2706338.1"
                     /translation="MTATSPAPGSPVQGELAGMPKRLYGASPSRLLMWLDCPRRYRMA
                     YLDRPRPRARPPRAHTSLGVAVHSALRDFWDVPIGRRLPARGDELVRRSWVTAGFRDA
                     EQSERWRAASATHVTSYLQGVDPARQPVGVERTVALKTSALAVTGRIDRLDERPAAGG
                     PGTELVVVDYKTSRAAPSAEDARTSLALALYAAAAWRMFRRRCVRVELHHVPTGQVVA
                     HEHTPDGLQRKIAEAESIASDLRRADADFAELGAGSRLFEPRTSPLCAWCDYRAYCPE
                     GQRMGPEKSSWAALEETT"
     gene            1118..1969
                     /locus_tag="VHM65_11310"
     CDS             1118..1969
                     /locus_tag="VHM65_11310"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX2706339.1"
                     /translation="MSATRARSGNAPGPATTAQPAAPGAAAYVIALANQKGGVAKTTS
                     VVSLGAAFAEAGLRVLLVDLDAQACLTFSLGVDPDTVERSVNEVLLGQCSAADAVVSC
                     SEGLDLLPSVIDLAGAEAALLPRPGREYVLRTALEPLFGSYDVILLDCSPSLGVLTLN
                     ALTAARGLVIPMPCEMLSHRGVGQLLDTVADVRRFLNRDLTVLGILPTLFDGRSNHSR
                     AVLSDVGERYGLPVLEPAIPKTVRFAEAPAVGRSILTTARTTKGAQAYRQVAAALMTR
                     IGRTARR"
BASE COUNT          269 a          872 c          825 g          323 t
ORIGIN      
        1 ccgtgcagcg ctccgacgat gcgggtgagc tggccgccac cgccctcggg ttcaccgccg
       61 ccatggtcgt cgtgtgggtc ttcctgcgct tcgcgagccg gatccgccgg gtgctgcgcg
      121 agagcggcac cgtgctcgcg acccggatcg ccgggttgct cctgtcggcg atcgcggtcc
      181 agatggtcgc cgacagtgtc cgcgcgttcg tcaccggagc cgcatagggt gaggcggtga
      241 ccgccacctc ccccgcgccc ggctcgcccg tgcagggcga gctcgcggga atgcccaagc
      301 ggttgtatgg cgcgagcccg agccgcctgc tcatgtggct cgactgccct cggcgctacc
      361 ggatggccta cctcgaccgg ccgcgcccca gggcccggcc gcccagagcc cacaccagcc
      421 tcggcgtcgc ggtccacagc gcgctgcggg acttctggga cgtgccgatc ggccgtcgac
      481 tccccgcgcg tggtgacgag ctggtccgcc ggtcgtgggt caccgcgggc tttcgtgacg
      541 ccgagcagag cgagcggtgg cgcgcggcga gcgcgacgca cgtgacgtca tacctgcagg
      601 gtgtcgaccc agcccgccag ccggtggggg tcgagcgcac cgtggcgctc aagacctccg
      661 ccctggccgt gacgggacgc atcgaccggc tcgacgagcg acccgcggcc ggcggcccgg
      721 gcaccgagct cgtcgtcgtc gactacaaga cgtcccgggc cgcgccgtcg gccgaggacg
      781 cgcggacctc gctggccctc gcgctctacg ccgccgcggc gtggcggatg ttccgtcgcc
      841 ggtgtgtccg ggtcgagctg caccacgtgc cgaccgggca ggtcgtcgcc cacgagcaca
      901 cgcccgacgg cctgcagcgc aagatcgccg aggccgagtc gatcgcgtcg gacctgcgcc
      961 gcgctgatgc cgacttcgcc gaactgggag caggatcacg cctgttcgag ccgaggacct
     1021 ccccgctgtg cgcctggtgt gactaccgtg cgtactgtcc ggaaggacag cggatgggcc
     1081 ccgagaagtc ctcgtgggcg gcactggagg agaccacatg agtgcgacac gggctcggtc
     1141 ggggaacgcc ccgggccccg cgacgacagc gcagcccgcg gcgccgggcg ctgcggccta
     1201 cgtcatcgcg ctggccaacc agaagggcgg cgtggccaag acgacctcgg tggtctcgct
     1261 cggagcggcc ttcgccgagg cgggcctgcg ggtgctgctc gtcgacctcg atgcgcaggc
     1321 gtgcctgacc ttcagcctcg gcgtcgaccc cgacacggtc gagcggtcgg tcaacgaggt
     1381 gcttctcggc cagtgctcgg cggcggatgc ggtcgtgtcg tgctccgagg gtctggacct
     1441 gctgccctcg gtcatcgacc tcgcgggcgc cgaggcggca ctcctgccgc ggccaggccg
     1501 cgagtacgtc ctgcggaccg ccctggagcc cctcttcggc tcgtatgacg tcatcctgct
     1561 cgactgttcg ccgtctctcg gcgtgctcac cctcaacgcg ctgacggctg cgcgggggct
     1621 cgtcatcccc atgccgtgcg agatgctgag tcaccgaggg gtgggccagc tgctcgacac
     1681 ggtcgccgac gtgcggcgct tcctcaaccg ggacctgacc gtgctgggca tcctgccgac
     1741 gctcttcgac gggcggagca accactcgcg ggcggtgctc tccgacgtcg gcgagcgcta
     1801 cggcctgccg gtgctcgaac cggcgatccc caagacagtg cgcttcgccg aggctcccgc
     1861 ggtggggcgc tcgatcctga ccacggcccg gacgaccaag ggtgcgcagg cctaccggca
     1921 ggtggcggca gcactcatga cgcgcatcgg gcggaccgcg cgccgataaa ccggctcccg
     1981 ctcacgactc gcgggatctg gcgctcagcc ctcggacgcc ggcgcagcgt tcgccgcgcc
     2041 ggcaccgccc gctgttcctg ggcgtccacc ccgagtccgt cggcggctcc gctcgcgggg
     2101 gcggccttcg ccctcgggct tggccgcggc agcctgaccc tcaccgcgac gcggccgggt
     2161 gctcggccgg tggccgccgt cgcggtcgcg gcggtcgtcg ccgccgccac gccggtcgcg
     2221 cccgccgcca ccatcaccgc ggggccccct gtccccgcga gggccaccgt cgccgcgacc
     2281 ctgcggccg
//
LOCUS       DATAIX010000765         1635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_267608, whole genome shotgun sequence.
ACCESSION   DATAIX010000765 DATAIX010000000
VERSION     DATAIX010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267608"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..450
                     /locus_tag="VHM65_11315"
                     /pseudo
     CDS             103..450
                     /locus_tag="VHM65_11315"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(744..1382)
                     /locus_tag="VHM65_11320"
     CDS             complement(744..1382)
                     /locus_tag="VHM65_11320"
                     /inference="COORDINATES: protein motif:HMM:NF024635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2706340.1"
                     /translation="MRFHVMHPADPAHVAAVLDLALSAQPRCGSTLVIAIDGPSGAGK
                     TTLARGVARSLGCPVVHLDDLYPGWDGLAEAVGLLTTQVLEPLRDGERAAYRRWDWEH
                     DRWAETIDVPPTPILVVDGVGSSVRPAGDFAAVRVWVDADLGTRRRRGIERDGEAYRP
                     HWERWAHQEQALFASDGTRARADLAFDTTLGPGPDAPAFETGGEPAGRGPTS"
     gene            1520..>1635
                     /locus_tag="VHM65_11325"
     CDS             1520..>1635
                     /locus_tag="VHM65_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX2706341.1"
                     /translation="MSVATRVIACLDVDAGRVVKGVNFTNLRDAGDPVELAK"
BASE COUNT          226 a          606 c          558 g          245 t
ORIGIN      
        1 gggccgagtg gtcacctacc tggtcgcggc acattgacat cccggctcgg tctcggcgcg
       61 gccgcggccg gggggctggc cggcttcgcg tactggctcc tcatgtcgcc ccactcgcag
      121 gtcctgggac ccttccccta tcgagcaacc accaccgagc gggtcgtcgc cttgaccttc
      181 gacgacgggc cgaacgagcc gtacacctcg cagatcgcgg acttcctcga ccggcgaggc
      241 gtcacggcga cgttcttcca ggtcgggcgt gctgtggttc gccagccggg ggtcaccgcc
      301 cgcctcgcag cgggcggaca cgtgatcggc ctccatggct acgctcacga gttcactcgt
      361 tacctgcgcc cacgaagtct cgccgcggac atgcagcagg gcctcgacgc gttcgccgag
      421 cttggactgc ggccggtgct gtaccggccg ccctggctcc tgcgcatccc cgccctgtgg
      481 ggaatctcgc gtcaccaccg gctgcaggtc atctccggag acttctgcca cgccctggag
      541 gtattccagc ccgccgcgga ccggatcgcc cgtcgcaccc tggccaagac caggcccggg
      601 tccatcctca tcttccacga cggcttcgac ggtcgaggtg gcgaccgcgc ccgcaccgtt
      661 gccgcggtca agatcgtcgt cgaccgactc atcagcctgg gatatggctt caccacggtc
      721 gaccgcttgc tcgagattcc cgcctacgac gtaggcccac ggccggcagg ctcgccaccg
      781 gtctcgaacg ccggggcgtc agggcccggg cctagcgtcg tgtcgaacgc caggtcggcc
      841 cgcgctcggg tgccatcgga ggcgaacagg gcctgctctt ggtgggccca gcgctcccag
      901 tgcgggcggt aggcctcgcc gtcgcgctcg atcccgcgtc gcctccgggt cccgaggtcg
      961 gcgtcgaccc agacccgcac ggccgcaaag tcaccggccg gccggacgct ggagccgacg
     1021 ccgtcgacga cgaggatcgg cgtcggtggc acgtcgatgg tctcggccca gcggtcgtgc
     1081 tcccagtccc atcgccggta tgccgcgcgc tcaccgtcgc gaagtggctc caggacctgc
     1141 gtcgtgagca ggccgacggc ctcggccagg ccgtcccagc cggggtagag gtcgtccagg
     1201 tggaccacgg ggcagccgag ggagcgggcc acacctcgcg cgagcgtggt cttgcccgcc
     1261 ccgctcggcc cgtcgatggc gatgacgagg gtcgaaccgc accggggctg cgctgacaag
     1321 gcgaggtcca ggacggccgc aacgtgtgcg gggtccgccg ggtgcatgac gtggaaccgc
     1381 acggcacctc ccggaacctg tcgcccaggg gccgccggcc ggcccgctgg ggttgcgatc
     1441 agagactatg cgccgagtca gtggacaacg gccagcagcg ggccgctcat cgctagcctg
     1501 actcgctagc ctgcatgaca tgagtgtcgc gacccgcgtc attgcctgcc tcgacgtcga
     1561 cgccgggcgc gtggtcaagg gcgtcaactt cacgaacctg cgcgacgccg gcgaccccgt
     1621 cgagctggcc aagat
//
LOCUS       DATAIX010000766         1750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_267712, whole genome shotgun sequence.
ACCESSION   DATAIX010000766 DATAIX010000000
VERSION     DATAIX010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267712"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..1444)
                     /locus_tag="VHM65_11330"
     CDS             complement(197..1444)
                     /locus_tag="VHM65_11330"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2706342.1"
                     /translation="MLPHEEVEMFVGIDWAADTHAICVEDAAGRVVDRFTIEHSAEGF
                     ERLVGRLARHGDPAALPVAIERPDGRLVDRLLEAGHPVVPVKTTAIKAWREAEVGSGA
                     KSDPGDARVIAEYLRLRGHDLRALHPFSAETRALRAVVRTRSDLVTARVAAGNQLSAT
                     LAAFWPGAGEVFADVWSGIGLAFLEQYPTPTAAGRLGEVRMAGFLRKHGYSGRRTPAE
                     LLGRLRVAPTGLDDGPESEARRDTVLAMVRVLRALNASIRDLDRSVAAHLGEHPDAEV
                     FRSLPRAGVTNAAQMLAEWGDCREAYDGPEAVAALSGMAPVTRRSGKHLAVGFRWACN
                     KRFRNAMTTFADNSRHASPWAAAVYAAAIDRGADHPHAVRILARAWIRVIHRCWLDHV
                     AYDPAKHRGATRLEVGSQVSVAA"
BASE COUNT          242 a          644 c          625 g          239 t
ORIGIN      
        1 gctcgatcgg ctggaagccg agaacgcggc gctgccgcag cgccgcgttc tcggcttcca
       61 gccgatcgag ccgctccagg atcccgtcga actccgacat cgctgtctcc ttcggtccgt
      121 ggtcgtaggc gacgaccgta gcccgggtga gcacgggtga caagcggtca cgcggatgac
      181 accctcagtg tcaaccctat gccgcgaccg agacctgcga gccgacctcc agccgcgttg
      241 caccacggtg cttcgccggg tcgtaggcga cgtggtcgag ccagcaacgg tggatgaccc
      301 ggatccaggc gcgggccagg atgcgcacgg cgtgggggtg gtcggcgcca cggtcgatgg
      361 ccgccgcgta gaccgccgcc gcccatggac tggcatgccg gctgttgtcg gcgaaggtcg
      421 tcatggcgtt gcggaagcgc ttgttgcacg cccagcggaa gcccaccgca aggtgcttgc
      481 ccgagcgccg ggtcacgggt gccatgcccg acagggcggc caccgcttcc ggtccgtcgt
      541 acgcttcccg gcagtcgccc cactcggcga gcatctgggc ggcgttggtg acgcctgctc
      601 gtggcagcga gcggaagacc tcggcgtccg gatgctcgcc cagatgggcg gccacggacc
      661 ggtcgaggtc ccggatcgac gcgttgagcg cgcgcagcac gcggaccatg gccaggacgg
      721 tgtcgcgacg cgcctcggac tcgggcccgt catcgaggcc ggtcggagcg acccgaagcc
      781 gcccgagcag ctcggccggg gtccgtcgcc cgctgtagcc gtgcttgcgc aggaagcccg
      841 ccatgcgcac ctcgcccagg cggccggccg cggtgggcgt ggggtactgc tccaggaagg
      901 cgaggccgat cccgctccac acgtcggcga acacctcgcc ggctcccggc cagaaggccg
      961 ccagggtcgc cgagagctgg ttgcccgctg ccacccgcgc ggtgaccagg tcgctccgcg
     1021 tacggacgac ggcgcgcagg gcccgcgtct cggcgctgaa cggatgcagc gcgcgcaggt
     1081 cgtgaccgcg cagccggagg tactccgcga tgacccgcgc gtccccggga tcgctcttgg
     1141 cacccgagcc gacctcggcc tctcgccagg ccttgatggc cgtggtcttg accggcacca
     1201 ccgggtgacc ggcctccagc agccggtcca ccagccggcc gtccggtcgc tcgatggcca
     1261 ccggcagggc agccgggtcc ccgtggcggg ccagtcgtcc gaccagccgc tcgaagccct
     1321 cggcgctgtg ctcgatggtg aagcggtcga ccacccggcc ggcagcatcc tcgacgcaga
     1381 tggcgtgcgt atccgccgcc cagtcgatcc cgacgaacat ctcgacctcc tcgtgtggca
     1441 gcacgagtcc ggtcccgagg acatctcccg ggtggtcact gactggcgct cgctggcgcg
     1501 tccctctgtc gccggtcgca ggtcctcggg ggaccggggg cggcggtgtc atccgggccc
     1561 tcgaagggcg accgacccag gccgtctccc cgatcctcac cgaacgacga tggccaacga
     1621 cggcctcgtc agctggaggg tagaccagtg accgtggccg ccgagccaac gacccgggcc
     1681 aggctccggc gcctgctggg ggtcatcgag gagctccagc cacggacctg gcgcgacggg
     1741 ccgttcacgc
//
LOCUS       DATAIX010000767        10251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_267725, whole genome shotgun sequence.
ACCESSION   DATAIX010000767 DATAIX010000000
VERSION     DATAIX010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 10251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10251
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267725"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VHM65_11335"
     CDS             <1..196
                     /locus_tag="VHM65_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706343.1"
                     /translation="PVSGQHRCLALVVAGIALTLLGLLAILSIGLPIVAAGIAPEDAS
                     GGPHGRNRPVELAKRLAIPA"
     gene            complement(661..1464)
                     /gene="hypB"
                     /locus_tag="VHM65_11340"
     CDS             complement(661..1464)
                     /gene="hypB"
                     /locus_tag="VHM65_11340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEX2706344.1"
                     /translation="MGRFHRHDDGTEHEHDDGTDHGPGDRTGQGPEWQVGDHTAYRTG
                     VDRISVLERIFGENDAAAAANRRALDAAGVRCVNLMSSPGAGKTALLVHTLAHLRSEG
                     IRAGVVVGDIETALDADRLSGAGAQISLVNTGHGFGGECHLDAPMVARGLASLDLHEL
                     DLVLVENVGNLVCPAEFDIGAHHRVMVSAVTEGEEKPLKYPVMFRSVELVLVNKIDLL
                     PYLDVDLALFETNLRAVNPRCDLIHVSARSGEGLADWYTWLAGRDGRHP"
     gene            complement(1457..1849)
                     /locus_tag="VHM65_11345"
     CDS             complement(1457..1849)
                     /locus_tag="VHM65_11345"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEX2706345.1"
                     /translation="MHELSLCCSIYDIVDRAREGRQVTTVELTVGQLRQVVPETLADC
                     WEVVTATTPLAGSRLRVEHVPGRLACPRCSAVTTLDTAFVFACAQCGALEVTVESGDE
                     LTVTAVDLVDVPAAGPDHAMAERKGADG"
     gene            1938..4460
                     /gene="hypF"
                     /locus_tag="VHM65_11350"
     CDS             1938..4460
                     /gene="hypF"
                     /locus_tag="VHM65_11350"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEX2706346.1"
                     /translation="MSAQDRQTAMDLASAVVRREYVLVGLVQGVGMRPFVARLARGLG
                     LAGLCRNSPSSVVVEVEGPPSAVEEFAARLVSQAPPLAVVDSVRERPIPPTGRPGFTI
                     AASGGTTGGRTLVAPDTAPCVDCLRELADPSDRRFRHPFITCTNCGPRFTITLDLPYD
                     RPATTMAGFPLCPACAREYADPADRRYHAQPIACHDCGPTLTLRSADGTAVARETEDV
                     LARARAALAGGAILAVKGLGGYHLACDAANPDTVGLLRRRKHRPDQPFAVMPADLATA
                     GRIAVLAESSTRLLLSPARPIVLVPRSPAAGRWVCDDVAPRVDDLGVLLPYTPVHLLL
                     MGDPPTGGSRVPRVLVMTSGNRSGEPLCVADEDALHRLQGIADLWLTHDRPIALACED
                     SVVALDAEHRPVPVRRSRGYAPIPVRLAHAEAGPHPVVLAAGADLKNTFTIVRDGMAF
                     VSAHVGDLGSLESRTGYHRAVDQAERFHRATPALVVADRHPGYLSRAWAAARSEALGV
                     PLLEVQHHHAHLAALAAEHGRLDIPLVGLVFDGTGYGCDATIWGGELLRLGPGGQRCE
                     RLGHLGTLPLPGGDAGVRNPVRMAAAALAAAGLPLDGTPVAEALTPGARRLLGSLPAG
                     RGTWVPTSSVGRLFDVVSSLLGVCHRVSYEAQAAIELEAVAARWARTVAPEGPVDLRD
                     LGSDLGTRRLTPLGMPVARGRDGPAVLDPLPLLADLTAAVRGGVDRGDLAWHFHAALA
                     DGAAELAAGAVAGTGEDTVGLTGGVFQNRLLSALTVLALARRGLRALTHRLVPPNDGG
                     ISLGQAAVGVASARAAHSGDDHLPDGGVGPVESADTRRGRHQ"
     gene            4457..5554
                     /gene="hypE"
                     /locus_tag="VHM65_11355"
     CDS             4457..5554
                     /gene="hypE"
                     /locus_tag="VHM65_11355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEX2706347.1"
                     /translation="MSGVAPSGPRSEAAAYERAESFRRRRPTLTEDVVTLAHGAGGSS
                     SAALIAAVFLDVFANPDLEPLADAAVLTLPGGGRLAFSTDSFVVTPLRFPGGSIGHLA
                     VHGTVNDLAVMGALPQWLSAAFVLEEGVSVDLLRGIVEDMQQAAVASGVTIVTGDTKV
                     VARGSADGLYITTAGVGLIPAGRRLEAGLVRPGDAVLVSGSMGRHGMSVMLARGDLAI
                     EADIVSDTAPLVGVVGELLRAAPGTRWLRDATRGGVGTVCNELARSAEVGVRLEEDLL
                     PVDATVRGACDLLGIDPLYVANEGLLVAVVPGDEADAALAALRGHPLSREAAVVGEVV
                     ADPVGIVALRTTFGGTRLVDMLVGDPLPRIC"
     gene            5632..5901
                     /locus_tag="VHM65_11360"
     CDS             5632..5901
                     /locus_tag="VHM65_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX2706348.1"
                     /translation="MCLGIPGQVVDIVDAEQYLAKVEVSGARRTVSLRLLADEGVGVG
                     DWVLVHVGFAMARIDEDEAAATLGVLEAMGREYLNELEAFRSTSG"
     gene            5924..7024
                     /gene="hypD"
                     /locus_tag="VHM65_11365"
     CDS             5924..7024
                     /gene="hypD"
                     /locus_tag="VHM65_11365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEX2706349.1"
                     /translation="MKYVDEFRDPAAARALVASIHGLAGADDPVAFMEICGGHTHTIY
                     RHGLEHLLPDSVELVHGPGCPVCVIPLGRVDDAIWLAEQPGVILTTFGDMLRVPGSTG
                     SLLQAKARGADCRIVYSPLDAVAVAVANPDRHVIFFAVGFETTAPSTAVTLQRARELD
                     LGNFSVFSNHVTIVPPITAILDAPQLRLDGFIGPGHVSAVVGTEVYRFVPEVYGKSIV
                     VSGFEPLDILQSVHMLMRQRAQGRCEVENQYTRVVRPEGNKEALGLLEETMQVRPSFE
                     WRGLGVIPRSGLKVHPDYARWDAEERFTVPGARVADPKACQCGDVLRGLIKPWECRVF
                     GTACTPQTPIGTCMVSSEGACAAYYTFGRFER"
     gene            7219..9027
                     /locus_tag="VHM65_11370"
     CDS             7219..9027
                     /locus_tag="VHM65_11370"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX2706350.1"
                     /translation="MERRIQAVVDRYAADRSRLLDMLWEVQDHWGYLPTQALDQLAAV
                     LGMSPVEVWSTATYYHFFHDRPAGTHRIYLADTVVAKMDGYRAVREALERETGATFGG
                     TDPSGMFGLFDTPCIGLSDQEPAMLVDKVVFTRLRPGKVADIIAELKAGKSAAEIANP
                     AGLPTDELAYVETLVESNVRSKGPVFFRRPPDYRALVRDCLVGQPEQVMESIAESGLR
                     GRGGAGFLTGLKWQLCSAAPGERKYVVCNADEGEPGTFKDRVLLTRSPKQVFVGMTIC
                     AFAIGGSAGIIYLRAEYWYLKDYLEAQLEEMRADGLLGQNIAGRRGFHFDIRIQMGAG
                     AYVCGEESALIESCEGRRGSPRVKPPFPAQKGYHGMPTCIDNVESFATVTSILEEGND
                     WFASLGTDESSGTRLLSVAGDCSRPGVYEIEWGVTLGQVLDLVGAPDAVAVQVSGPSG
                     ECVNVATEADRQIAYEDLSCNGALTIFNKDRDLLAIARDYLQFFIGESCGICTPCRVG
                     NVALRDKLDLVIAGRASQQDLDDMVRWGAMVRAMSRCGLGGTSPKPILTTMEKFPELY
                     RTRLVKETTALLPSFDLAAAFAGQESGSPNGGRRTS"
     gene            9036..9728
                     /locus_tag="VHM65_11375"
     CDS             9036..9728
                     /locus_tag="VHM65_11375"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX2706351.1"
                     /translation="MSIDGEAVEAEVGQTLVVAAAAHGVYIPTICSLPDQPSIGTCRV
                     CSVRVNGQVMAACTIRVRDGLVVEVNAPDLADMRRAVVELLFVEGNHNCPSCEKSGRC
                     QLQAVAYEVGMTSARFPYRYPVREADDSSQRIWLERDRCIFCQRCVELIHDRDTGRKV
                     FSISHRGPAARIEIDTELADAMTDEQVKEAVAICPVGSILEKRVGHDTPIGERRFEVR
                     SVRSRALGGGQP"
     gene            9725..>10251
                     /locus_tag="VHM65_11380"
     CDS             9725..>10251
                     /locus_tag="VHM65_11380"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706352.1"
                     /translation="MTDHVDGVDHGTASHELPRTPFPPEQAALRAGKIKVSTISLCGC
                     WGCTLSILDVDERILGLLDEVTILRSSLTDIKRIPERCAVGFVEGGVANEENIETLVH
                     FRENCDILISVGACAVWGGVPSLRNIYPLSEVLATAYLDSPTAVKGAPPVIPRHPEIP
                     RLTAKVFPCHEVVHM"
BASE COUNT         1388 a         3773 c         3602 g         1488 t
ORIGIN      
        1 ccccgtctcc ggtcagcacc gctgcctcgc cctggtggtt gccgggatcg ccctcaccct
       61 gctggggctg ctcgcgatcc tgtcgatcgg tctgccgatc gtggccgccg gcatcgcgcc
      121 ggaagacgcc agcggtgggc cgcacggccg caaccggccc gtcgaactgg ccaaacgact
      181 cgccatacca gcgtgaatag cttcaaacca ggacataacc acctggcatg gagcgcttcg
      241 gcctgtttgg atgtccggcg acaccgatgt ccggcgacag tgtcggcgag gagccggtgt
      301 tggcgcggtg gatgcttgcc ggcgtggaga cgactgacct gaccgatgtc gagcgtcggc
      361 ttgtgcagtc cgcggtggcc gggacggtgc tggacctggc tggggacgtc ccgaccgaca
      421 tggacacgat ggccgcgggg ggggtccgga ccggcaggtt cgggcactgg ttgtgcggga
      481 gctcctcctc ggccgggtcg acgaagctcc gacgccggac cccaagggcg tgcgtgccgc
      541 gggctggtgg tcaccggctg ggtcgacctc gactactgca cgacgaccgt gccgctggtc
      601 ctcgaggaca ggccgcctcg acgggggact cagcgaccgg aacccgggcg ccacccgtcc
      661 ttacgggtgg cgcccgtccc gtccggccag ccacgtgtac cagtcggcca gcccctcacc
      721 ggaccgcgcg ctgacgtgga tgaggtcgca gcgcgggttg accgcccgga ggttggtctc
      781 gaagagggcc aggtcgacat cgaggtaggg gagcaggtcg atcttgttga ccagcacgag
      841 ctccaccgag cggaacatga cggggtactt cagcggcttc tcctcgccct cggtcaccgc
      901 cgacaccatg acccggtggt gggcgccgat gtcgaactca gccgggcaga cgaggttccc
      961 gacgttctcg acgaggacca ggtcgagctc gtggagatcg agcgacgcca ggccccgggc
     1021 caccatcggc gcgtcgaggt gacactcgcc accaaacccg tgcccggtgt tgaccaggct
     1081 gatctgtgcg ccggcgccgc tgagccggtc ggcatccagg gcggtctcga tgtccccgac
     1141 gacgaccccg gcccggatgc cctcgctgcg caggtgggcg agggtgtgga cgagcagggc
     1201 cgtcttgccc gcgcccgggg aggacatgag gttgacgcag cgcacccctg ccgcgtcgag
     1261 ggcccgacgg ttggccgcgg ccgcggcgtc gttctccccg aagatccgct cgagcacgct
     1321 gatccggtcc accccggtcc ggtatgccgt gtggtcgcct acctgccact cgggcccctg
     1381 gccggtgcgg tcgccgggcc cgtggtcggt gccgtcgtcg tgctcgtgct cggtgccgtc
     1441 gtcgtgccgg tggaacctac ccatccgcgc ccttccgctc tgccatggca tggtccggac
     1501 ccgcggccgg cacgtcgacg aggtccacgg cggtcacggt gagctcgtcc ccggactcga
     1561 ccgtcacctc gagcgccccg cactgggcgc aggcgaagac gaacgccgtg tcgagcgtgg
     1621 tcaccgccga gcaccgcgga caggcgagcc gccccggcac gtgctcgacc cgcagacgcg
     1681 agccggcgag cggggtggtc gcggtgacca cctcccaaca gtcggccagc gtctcgggga
     1741 caacctgtcg gagctgaccg acggtcagct cgaccgtggt cacctggcgg ccctcgcgcg
     1801 cccgatcgac gatgtcgtag atcgagcagc acagggagag ctcgtgcata gccacaccat
     1861 ggcacgttcc gggcgacccg tcgcccccga aggaggcaga ctctggtgca gccacgtcgt
     1921 gcccgaacgg aggaccggtg agtgcgcagg atcggcagac cgcgatggac ctggccagcg
     1981 ccgtggtccg ccgcgagtac gtcctcgtcg ggctcgttca gggtgtcggg atgcgcccct
     2041 tcgtcgcccg gctcgcccgc gggctcggcc tggccgggct ctgccggaac tccccgagca
     2101 gcgtcgtcgt cgaggtcgag gggccgccgt cagcggtcga ggagttcgcg gcccggttgg
     2161 tgagccaggc accgccgctc gccgtcgtcg actcggttcg cgagcggccc atcccgccga
     2221 ccggtcggcc ggggttcacc atcgccgcga gcggtgggac gaccggtggg cggaccctcg
     2281 tcgccccgga caccgctccg tgcgtcgact gcctgcgcga gctcgccgac ccgtccgacc
     2341 gccgcttccg ccacccgttc atcacgtgca cgaactgcgg gccgcggttc accatcaccc
     2401 tcgaccttcc ctacgaccgg ccggcgacga cgatggcggg gttccccctg tgcccggcct
     2461 gcgcccggga gtatgccgac ccggccgacc gccgatacca cgcccagccg atcgcctgcc
     2521 acgactgcgg acccaccctc accctgcgca gcgccgacgg cacggccgtg gcccgggaga
     2581 ccgaggacgt cctcgcgcgg gctcgggccg cgctggccgg cggcgcgatc ctcgccgtca
     2641 aagggctcgg cggctaccac ctggcctgcg acgcggccaa cccggacacg gtcggcctgt
     2701 tgcgccggcg caagcaccgg cccgaccagc cgttcgcggt catgcccgcg gacctggcga
     2761 ccgccggacg gatcgccgtg ctcgccgaga gctcgacccg gctgctcctc agcccggcac
     2821 ggccgatcgt cctcgtgccg cgcagcccgg cggccggtcg atgggtctgc gacgacgtcg
     2881 ccccccgggt cgacgacctc ggggtgctcc tgccctacac accagtgcac ctgctcctca
     2941 tgggcgaccc gccgacgggg ggctcgcggg tcccccgagt cctcgtcatg acctcgggca
     3001 accgcagcgg ggagcccctt tgcgtcgccg acgaggacgc cctccatcgc ctgcagggca
     3061 tcgccgacct ctggctcacc cacgaccggc cgatcgcgct cgcctgcgag gactcggtcg
     3121 tcgcactcga cgccgagcac cgcccggtcc cggtgcgccg ttcccgtggc tacgccccga
     3181 tccccgtccg gctcgcccac gccgaggcgg gaccacaccc cgtcgtcctc gccgccggcg
     3241 ccgacctcaa gaacaccttc accatcgtcc gcgacgggat ggcattcgtc tccgcgcacg
     3301 tcggggacct ggggagcctg gagagccgga ccgggtacca ccgagccgtc gaccaggccg
     3361 agcgcttcca ccgggcgaca cccgcgctcg tcgtggccga ccggcatccg ggctacctga
     3421 gccgcgcctg ggcagcagcc cgtagcgagg cgctcggcgt cccgctgctc gaggtccagc
     3481 accaccacgc ccatctggcc gccctcgccg ccgagcacgg ccggctcgac ataccgctgg
     3541 tcgggctcgt cttcgacggc accgggtacg gatgcgacgc gaccatctgg gggggcgagc
     3601 tgctccggct cgggccgggg ggccagcgct gcgagcggct cggtcacctc gggacgctcc
     3661 cgctgccagg gggcgacgcc ggagtgcgca acccggtccg gatggccgcc gcggcactcg
     3721 ccgccgccgg cctccccctc gacggcacgc cggtcgccga ggccctcacc cccggcgcgc
     3781 ggcggctcct cgggtcgctg ccggcgggcc ggggcacgtg ggtccccacg agcagcgtgg
     3841 ggcggctctt cgacgtcgtc tcctccctgc tcggagtgtg tcaccgcgtc agctacgagg
     3901 cccaggccgc catcgagctc gaagcggtgg ccgcccggtg ggctcgcacg gtcgcccccg
     3961 agggccccgt cgacctgcgc gacctcggct ccgacctggg cacgcggcgc ctgacaccac
     4021 tgggtatgcc ggtcgctcgc ggccgcgacg gcccagcggt gctcgacccc ctgccgctgc
     4081 tcgcggacct caccgctgcg gtccggggcg gagtggaccg cggcgacctg gcgtggcact
     4141 tccacgccgc cctcgcggac ggcgcggccg agctcgccgc cggcgcggtc gcgggcaccg
     4201 gcgaggacac ggtcgggctg accggggggg tcttccagaa ccggctgctc agcgccctga
     4261 cggtgctcgc gctggcccgg cgcgggctcc gggcgctgac ccatcgtctc gttcccccca
     4321 acgacggcgg gatcagcctc ggacaggccg cggtgggagt cgcctccgcg cgggccgctc
     4381 actccgggga cgaccacctc ccggacggcg gggtcgggcc ggtagagtcg gccgacacca
     4441 ggcgagggag gcaccagtga gcggagtggc accgtccggg ccacggagcg aggcggcggc
     4501 atacgagcgg gcggagtcct tccgccgccg tcggccgacg ctcaccgagg acgtcgtcac
     4561 cctcgcccac ggagccggcg gctcgtcctc cgcggccctg attgccgcgg tcttcctcga
     4621 cgtcttcgcc aaccccgacc tcgagccgct cgccgacgcg gcggtcctca ccctgcccgg
     4681 gggaggccgg ctcgcgttct ccaccgactc cttcgtcgtc accccgctgc gcttcccggg
     4741 cgggtcgatc ggccacctgg ccgtgcacgg gaccgtcaac gatctcgccg tcatgggggc
     4801 gctgccgcag tggctctcgg cggcgttcgt cctcgaggag ggggtgtccg tggacctgct
     4861 gcggggcatc gtcgaggaca tgcagcaggc cgccgtggcg tccggagtca ccatcgtcac
     4921 cggggacacg aaggtcgtcg cgcgaggctc cgcggacggg ctctacatca cgaccgccgg
     4981 ggtcggcctc atccccgccg gccgccggct cgaggcgggg ctggtccgcc ccggcgacgc
     5041 cgtcctcgtc tccgggtcga tgggtcggca cgggatgtcg gtcatgctcg cccgtgggga
     5101 cctcgccatc gaggcggaca tcgtctcgga caccgcccca ctcgtcggcg tcgtcggcga
     5161 gctcctccgg gcggcgcccg gcacccggtg gctgcgggac gccacccgtg gcggggtcgg
     5221 caccgtctgc aacgagctgg cccgcagcgc cgaggtcggg gtccggctcg aggaggatct
     5281 gctgcccgtc gacgcgaccg tccgaggtgc ctgcgacctg ctcggcatcg acccgctcta
     5341 cgtggccaac gaggggctgc tcgtcgcggt ggtgcccggc gacgaggcgg acgcggcgct
     5401 ggccgccctg cgcggacacc cgctctcgcg ggaggccgcc gtcgtcggtg aggtcgtcgc
     5461 cgacccggtc ggtatcgtcg cgctgagaac gacgttcggc gggacccggc tcgtcgacat
     5521 gctcgtcggg gacccgctcc cgcggatctg ctgaatgccc ggtgcagacc gtcatactcg
     5581 ttccaggacc atactcgttc caggacgtca tactcgcccg aggaggccgc catgtgcctg
     5641 gggatacccg gccaggtcgt cgacatcgtc gacgccgagc agtacctcgc caaggtcgag
     5701 gtgtccggcg cgcggcgcac ggtgagcctg cgcctgctcg ccgacgaggg ggtcggcgtc
     5761 ggcgactggg tgctcgtcca cgtggggttc gccatggccc ggatcgacga ggacgaggcg
     5821 gcggccaccc tcggcgtgct cgaggcgatg ggacgggagt acctcaacga gctcgaggcc
     5881 ttccgctcca cctccggatg acggggcggt caggaaggct gccatgaagt atgtcgacga
     5941 gttccgcgac ccggccgccg cgcgcgccct cgtcgcctcg atccatggcc tggccggcgc
     6001 ggacgacccg gtcgcgttca tggagatctg cggggggcac acccacacga tctaccggca
     6061 cggcctggag cacctgctgc ccgactccgt cgagctcgtc cacggccctg ggtgcccggt
     6121 ctgcgtcatc cccctcggac gggtcgacga cgcgatctgg ctcgcggagc agcccggcgt
     6181 catcctcacg accttcgggg acatgctccg ggtccccggc tcgaccggct cgctcctgca
     6241 ggccaaggcg cgcggtgccg actgccggat cgtctactca ccgctcgacg ccgtcgccgt
     6301 cgccgtcgcc aaccccgacc gtcacgtcat cttcttcgcg gtcggtttcg agacgaccgc
     6361 cccgtcgacc gcggtgaccc tgcagcgggc cagggagctc gacctgggga acttctccgt
     6421 gttctccaac cacgtgacga tcgtgccgcc catcaccgcg atcctcgacg ccccccagct
     6481 gcggctcgac gggttcatcg gccccggcca cgtctcggcg gtcgtcggga ccgaggtcta
     6541 ccggttcgtc ccggaggtgt acggcaagtc catcgtcgtc tccggcttcg agccgctcga
     6601 catcctccag tcggtgcaca tgctcatgcg gcagcgggcg cagggtcgct gcgaggtcga
     6661 gaaccagtac acccgggtcg ttcgccccga gggcaacaag gaggcgctcg ggctgctcga
     6721 ggagacgatg caggtccggc cgtccttcga gtggcgtggc ctcggggtca ttccccggtc
     6781 cgggctcaag gtccaccccg actacgcgag atgggacgcc gaggaacggt tcaccgtccc
     6841 cggcgcccga gtcgccgacc ccaaggcgtg ccagtgcgga gacgtcctgc gcggcctcat
     6901 caagccgtgg gagtgccggg tgttcggcac cgcgtgcacg ccccagacgc cgatcggcac
     6961 gtgcatggtg tcctccgagg gtgcgtgcgc cgcctattac acgttcggcc gcttcgagcg
     7021 gtagcgacgc gccaccgagc ggagccaccc gtcccgctcg gcgaccgaga tggccggtga
     7081 ccgcgtgggg ccgatctcgg cactcggccc gagaccccct cggctagcgt gagaagagca
     7141 tcggcacgaa gtccctgcgg agttgccttc tcggtgtccg ggcccgaccc gcccggccac
     7201 gcggatcatg gagcgagcat ggagcgtcgg attcaggctg tggtcgatcg gtatgccgcc
     7261 gatcggagtc ggctgttgga catgctctgg gaggtccagg accactgggg ctacctgccg
     7321 acgcaggcgc tcgaccagct cgcggccgtc ctgggcatgt caccggtcga ggtgtggtcg
     7381 acggccacct actaccactt cttccacgac cggcccgcgg gcacccaccg gatctacctc
     7441 gccgacaccg tcgtggcgaa gatggacggc tatcgggcgg tccgtgaggc gctcgagcgg
     7501 gagaccggag cgacgttcgg agggaccgac ccgagcggga tgttcggctt gttcgacacc
     7561 ccgtgcatcg ggctgagcga ccaggagccg gcgatgctcg tcgacaaggt ggtcttcacc
     7621 cggctgcgcc ccggcaaggt cgccgacatc atcgccgagc tcaaggcggg caagtccgcg
     7681 gccgagatcg ccaaccccgc cgggctgccc accgacgagc tcgcgtatgt cgagaccctc
     7741 gtcgagtcga acgtccggtc caaggggccg gtgttcttcc ggcgcccccc ggactaccgg
     7801 gcgctggtgc gggactgcct cgtggggcag cccgagcagg tcatggagtc gatcgccgag
     7861 tccgggctgc ggggtcgtgg cggcgccggg ttcctcaccg ggctgaagtg gcagctgtgc
     7921 agtgccgccc cgggcgagcg caagtacgtc gtgtgcaacg ccgacgaggg ggagccgggc
     7981 accttcaagg accgggtcct gctcacccgg tctccgaagc aggtcttcgt cgggatgacg
     8041 atctgcgcgt tcgccatcgg cggctcggcc gggatcatct acctccgcgc cgagtactgg
     8101 tacctcaagg actacctcga ggcccagctc gaggagatgc gtgcggacgg gctgctcggg
     8161 cagaacatcg ccgggcgtcg aggcttccac ttcgacatcc ggatccagat gggcgccggc
     8221 gcctacgtgt gcggcgagga gtccgccctc atcgagtcct gcgagggccg gcgcggctcg
     8281 ccgcgggtca aaccgccctt ccccgcgcag aagggctacc acggtatgcc gacgtgtatc
     8341 gacaacgtcg agtcgttcgc caccgtgacg agcatcctcg aggagggcaa cgactggttc
     8401 gccagcctcg ggaccgacga gtcgtcgggg actcggctgc tgtcggtggc cggcgactgc
     8461 agccgtccgg gcgtctacga gatcgagtgg ggggtcaccc tcggccaggt tctcgatctc
     8521 gtgggagcac ctgacgccgt ggccgtccag gtgagcgggc cgtcggggga gtgcgtcaac
     8581 gtcgcgaccg aggccgaccg gcagatcgcc tacgaggacc tctcgtgcaa cggggccttg
     8641 acgatcttca acaaggaccg cgacctgctc gcgatcgcgc gcgactacct gcagttcttc
     8701 atcggtgagt cgtgcggcat ctgtacaccg tgccgggtcg gcaacgtcgc tctgcgggac
     8761 aagctcgacc tcgtcatcgc cgggcgggcc tcccaacagg acctcgacga catggtgcgg
     8821 tggggcgcga tggtccgggc catgagccgg tgcggcctcg gtgggacgtc accgaagccg
     8881 atcctcacca ccatggagaa gttccccgag ctctaccgca cgcggctggt caaggagacg
     8941 acggcgctgc tgccctcctt cgacctcgcc gccgcattcg ccggccagga gagcgggagc
     9001 ccgaacggcg ggaggcggac ctcatgaacg tccagatgag catcgacggc gaggccgtcg
     9061 aggccgaggt cggccagacc ctggtcgtcg cggcggccgc ccacggggtc tacatcccga
     9121 ccatctgctc gctgcccgac cagccgagca tcggcacctg tcgggtctgc tcggtgcggg
     9181 tcaacgggca ggtcatggcc gcctgcacga tccgggtccg cgacgggctc gtcgtcgagg
     9241 tcaacgcccc cgacctcgcg gacatgcgtc gggcggtcgt cgagctcctt ttcgtcgaag
     9301 gcaaccacaa ctgccccagc tgcgagaaga gcggccggtg ccagctgcag gccgttgcct
     9361 acgaagtggg gatgacatcg gcccgcttcc cctaccggta cccggtgcgc gaggccgacg
     9421 acagctcgca gcggatctgg ctcgagcggg accgctgcat cttctgccag cgctgcgtcg
     9481 agctcatcca cgaccgcgac accggccgga aggtgttctc gatcagccac cgcggtccgg
     9541 ccgcgcggat cgagatcgac accgagctgg ctgacgccat gaccgacgag caggtcaagg
     9601 aggcggtggc gatctgtccg gtcgggtcca tcctcgagaa gcgcgtcggt cacgacaccc
     9661 cgatcgggga acgccggttc gaggtccgct cggtccgttc ccgcgcgctg ggagggggcc
     9721 agccatgacc gaccatgtcg acggcgtcga ccacggaacc gcgtcgcacg agctgccgcg
     9781 gacccccttc ccgcccgagc aggctgcact tcgcgccggc aagatcaagg tctcgacgat
     9841 ctcgttgtgc ggctgctggg gctgcacgct gtccatcctc gacgtggacg agcgcatcct
     9901 ggggctcctg gacgaggtga cgatcctgcg ctcctccctc accgacatca agcgcatccc
     9961 cgagcggtgc gcagtcgggt tcgtcgaggg cggggtagcc aacgaggaga acatcgagac
    10021 cctcgtccac ttccgggaga actgcgacat cctcatctcc gtgggagcct gcgccgtgtg
    10081 gggcggcgtg ccgtcactgc gcaacattta cccgctgtcc gaggtgctcg cgacggcata
    10141 cctcgacagc ccgaccgcgg tgaaaggggc cccgccggtc attccgaggc acccggagat
    10201 tccccgcctg accgccaagg tcttcccgtg ccacgaggtc gtccacatgg a
//
LOCUS       DATAIX010000768         3953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_147417, whole genome shotgun sequence.
ACCESSION   DATAIX010000768 DATAIX010000000
VERSION     DATAIX010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147417"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2650)
                     /locus_tag="VHM65_11385"
     CDS             complement(<1..2650)
                     /locus_tag="VHM65_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEX2706353.1"
                     /translation="MTHKEVRPVAIVGLSAIMPQAPNSDAFWANIKNGRYSITDVPAE
                     RWDPALYFDPDHLARDKTYSKIGGWVREFAWDPIKWRLPIPPTVSKQMDDGQQWAVSG
                     SRSALMDAGWPKWSVDTERVAVIFGNALGGEKHYKSAMRIQLPEMLKRLGQSATLLSL
                     TPETQQRVIEETRAAYLDNMFEITEDTMPGELANVMAGRVANLFNLRGPNYTTDAACA
                     SGLAALSSAVHGLVEHDYDAVVTGGIDRNMGVDGFVKFCKIGALSASGTRPFDAGADG
                     FVMGEGCALFVLKRLEDAERDNDRIYSVILGVAGASDGKGKGITAPNPAGQKLAIARA
                     WENAALDPATAGAVEAHGTSTRVGDAAELDSLTAVFGPSGAAPRSVALGSVKSNIGHL
                     KGAAGSAGLFKMVRSLHEKVLPPSLHFVNPNPNVDWDTVPFAVNTALRDWQAPASGVR
                     VGAVSAFGFGGTNFHVVLEEHVPGRHKPAAKVFASAQVPVAPVVSASVPAAPAGPAKT
                     PLRGALVLGGRDDADLMAQIQPVLAAAKAGSAPATAAPDPAVGQAAVRIAVDYGDAAE
                     LAAKLDKLVKALASQSAALFRMLRQQGVFVGRGPAPKVAFLYTGQGSQYVNMLKDLAA
                     AEPIVRDTFADADRVMTPLLGRPLTSYIFIDGDDEAAVKKLNKQLMQTEITQPAVLAA
                     DLALTSLLSAYGIKPDMVMGHSLGEYGALVAAGSLSFDAALEAVSARGHEMTKVSMDD
                     NGAMAAVFGPLAEIERVVAEAEGYVVIANINSSSQAVIGGATDAVETVVKTFVDEGIN
                     AVRIPVSHAFHTKIVAPASEHFVNALRRLTVVGPSLPIVANVTGEFYPMHADTETMLD
                     YVGKQIAAPVQFVKGLNTLYDAGARVI"
     gene            complement(2688..>3953)
                     /locus_tag="VHM65_11390"
     CDS             complement(2688..>3953)
                     /locus_tag="VHM65_11390"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2706354.1"
                     /translation="DLGALRTVAVPVEDDGKVVGYKITGRKQWISNGTIADATTILAL
                     APDGPTWFVVAKGTPGFGSAPPEDKHGIRLSNTAALYLDDVYVPIENLLGGVEGRGLI
                     QAQQVFGYTRLMVAAFGLGGGWEALDRAIQYSINRVQGGGPLCEKQGYTHKLIVPHAI
                     RLEAARAFMEDTADRIDHGFGENGAMNCEGAIAKYLATEAGNAAADAAIQAHGGYGYT
                     RPYVVEKVRRDVRITTIYEGTSEIMEMTVSRERWQQHLKTQGRYYTDKAAELTALHAR
                     RPEVGAGVAAFAAQALAVVLEACRVGRLTRNQHILFRIGELMCAAEGAGVFAFAAAAV
                     IDGTEFEKCLHRFDGAAMAAMARVFAREAAQKVAFDGARWVAGAADPGTAAVAALNAI
                     PTGAILAAQAGLVSDMNYVADVLYARTGA"
BASE COUNT          560 a         1315 c         1444 g          634 t
ORIGIN      
        1 gtatgacgcg ggcgcccgcg tcatacaggg tgttgaggcc cttgacgaac tggaccggtg
       61 ccgcgatctg cttgccgacg tagtcgagca tcgtctcggt gtcagcgtgc atggggtaga
      121 actccccggt cacgttggcc acgatcggca gcgacggccc caccacggtc aggcggcgca
      181 gcgcgttgac gaagtgctcg gaggccgggg cgacgatctt ggtgtggaag gcgtggctga
      241 ccgggatccg caccgcgttg atgccctcgt cgacgaaggt cttcacgacc gtctcgaccg
      301 cgtcggtggc cccgccgatg acggcctggc tggaggagtt gatgttggcg atgacgacgt
      361 agccctcggc ctcggccacg accctctcga tctcggccag cggaccgaag accgcggcca
      421 tggcaccgtt gtcgtccatc gacaccttgg tcatctcgtg cccgcgggcg ctgaccgcct
      481 ccagggccgc gtcgaagctc agcgacccgg cggcgacgag ggcgccgtac tcaccgaggc
      541 tgtggcccat caccatgtcg ggcttgatgc cgtaggcgct gagcaggctg gtgagcgcca
      601 ggtcggccgc gaggacggcc ggctgggtga tctcggtctg catgagctgc ttgttgagct
      661 tcttgaccgc ggcctcgtcg tcgccgtcga tgaagatgta tgacgtcagc ggtcgcccga
      721 gcagcggggt catgacccgg tcggcgtcgg cgaaggtgtc gcggacgatc ggctcggcag
      781 ccgcgaggtc cttgagcatg ttgacgtact gagacccctg gccggtgtag aggaacgcca
      841 ccttgggggc cgggccacga ccgacgaaca cgccctgctg gcgcagcatc cggaacagcg
      901 ctgcgctctg cgaggcgagg gccttgacga gcttgtcgag cttggcggcc agctcggccg
      961 cgtcgccgta gtcgaccgca atgcgcaccg cggcctggcc gacggccggg tcgggagccg
     1021 ctgtcgcagg ggcggacccg gccttggccg ccgcgaggac tggctggatc tgggccatga
     1081 ggtcggcgtc gtcgcggccg ccgaggacca gggcgccccg caggggcgtc ttggccggcc
     1141 ctgcgggcgc ggccgggacc gacgcgctca cgacgggcgc gaccggcacc tgggccgagg
     1201 cgaagacctt ggccgcgggc ttgtggcgcc cggggacgtg ctcctcgagg acgacgtgga
     1261 agttcgtgcc accgaaaccg aaggcgctga cggcaccgac gcgcacgccc gaggccggag
     1321 cctgccagtc acgcagggcg gtgttgacgg cgaagggcac ggtgtcccag tcgacgttgg
     1381 gattggggtt gacgaagtgc aggctcggcg ggaggacctt ctcgtgcagc gagcggacca
     1441 tcttgaacag gccggccgat ccggcggcgc ccttgaggtg gccgatgttg gacttgaccg
     1501 acccgagagc gaccgagcgc ggtgcggcac ccgagggacc gaagaccgcg gtgaggctgt
     1561 cgagctcggc ggcgtcgccg accctcgtcg aggtcccgtg cgcctcgacg gcgcccgcgg
     1621 tggcagggtc cagagcggcg ttctcccacg cgcgggcgat cgcgagcttc tgcccggccg
     1681 ggttgggggc ggtgatcccc ttgcccttgc cgtcggaagc ccccgcgacg ccgaggatca
     1741 ccgagtagat ccggtcgttg tcgcgctcgg cgtcctcgag gcgcttgagg acgaagaggg
     1801 cacagccctc ccccatgacg aacccgtctg ctccggcgtc gaagggccgg gtgccggagg
     1861 ccgagagtgc gccgatcttg cagaacttga cgaaaccgtc gacgcccatg ttgcggtcga
     1921 tgcccccggt gaccaccgcg tcgtagtcgt gctcgacgag cccgtggacg gccgagctga
     1981 gcgccgccag acccgaggca catgccgcgt cggtcgtgta gttcggcccg cgcaggttga
     2041 acaggttggc cacgcggccg gccatgacgt tggcgagctc accgggcatg gtgtcctcgg
     2101 tgatctcgaa catgttgtcc aggtatgccg cgcgggtctc ctcgatgacg cgctgctgcg
     2161 tctccggcgt cagcgacagc agtgtcgccg actgcccgag ccgcttgagc atctcgggca
     2221 gctggatgcg catggcggac ttgtagtgct tctcgccgcc gagggcgttg ccgaagatga
     2281 ccgcaacccg ctcggtgtcg acgctccact tgggccagcc ggcgtccatc agggccgacc
     2341 gggagcccga gaccgcccat tgctgcccgt cgtccatctg cttggacacg gtcggcggga
     2401 tcggcaagcg ccacttgatc gggtcccacg cgaactcgcg gacccagccg ccgatcttgg
     2461 agtaggtctt gtcccgggcc aggtggtcgg ggtcgaagta cagcgcgggg tcccagcgct
     2521 ccgctgggac atccgtgatg ctgtagcgcc cgttcttgat gttggcccag aaggcgtcgg
     2581 agttgggggc ttggggcatg atcgccgaca gcccgacaat ggcaaccggg cgaacttctt
     2641 tgtgagtcat cgtgaaggtc cttctcggag aagtttccgg atcttggtca ggcgccggtg
     2701 cgtgcgtaca gaacgtccgc gacgtagttc atgtcagaaa cgaggcccgc ctgcgcagcg
     2761 aggatcgcac ccgtggggat ggcgttgagg gcagccaccg cggcggttcc cgggtctgcg
     2821 gcaccggcga cccaacgggc gccgtcgaag gcgaccttct gggcggcctc ccgggcgaag
     2881 acccgcgcca tcgcggccat cgcggcaccg tcgaagcggt gcaggcactt ctcgaactcc
     2941 gtgccgtcga tgaccgcagc ggcggcaaag gcgaacacgc ccgccccctc ggcggcgcac
     3001 atgagctcgc cgatgcggaa gaggatgtgc tggttgcggg tcaggcgccc gacccggcac
     3061 gcctcgagca cgaccgcgag cgcctgggcc gcgaacgccg cgacgccggc gccgacctcg
     3121 ggtcggcgag cgtgcagcgc ggtgagctcg gcggccttgt cggtgtagta gcggccctgc
     3181 gtcttgaggt gctgctgcca gcgttcgcgc gagacggtca tctccatgat ctcggaggtg
     3241 ccctcgtaga tcgtcgtgat ccgcacgtcg cggcggacct tctccacgac atacgggcgg
     3301 gtgtagccgt agccgccatg ggcttggatc gcggcgtcgg cagcggcgtt gccggcctcg
     3361 gtcgcgaggt acttggcgat cgcgccttcg cagttcatcg cgccgttctc gccgaagccg
     3421 tggtcgatcc ggtcggccgt gtcctccatg aacgcccgcg ccgcctcgag ccggatggcg
     3481 tgcgggacga tgagcttgtg cgtgtagccc tgcttctcgc acagcgggcc gccgccctgc
     3541 acccggttga tggagtactg gatcgcccgg tcgagcgcct cccagccgcc gccgagcccg
     3601 aacgccgcga ccatgagccg ggtgtagccg aagacctgct gggcctggat cagcccgcgc
     3661 ccctcgaccc cgccgagcag gttctcgatc gggacgtaga cgtcgtccag gtacagcgcc
     3721 gcggtgttgg acagccggat gccgtgcttg tcctcgggcg gggccgaacc gaatccaggg
     3781 gtgccctttg cgaccacgaa ccaggtcggg ccgtccgggg ccagcgccag gatggtggtg
     3841 gcgtcggcga tggtcccgtt gctgatccac tgcttgcgcc cggtgatctt gtaaccgacg
     3901 accttgccgt cgtcctcgac cgggacggcg accgttctga gcgcgccgag gtc
//
LOCUS       DATAIX010000769         2274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_267860, whole genome shotgun sequence.
ACCESSION   DATAIX010000769 DATAIX010000000
VERSION     DATAIX010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267860"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="VHM65_11395"
     CDS             <1..1076
                     /locus_tag="VHM65_11395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX2706355.1"
                     /translation="LAVAAPITQAVRYAAISRDTMSEIFVATSTAAPSVRPAAAEVDP
                     WAGTARVSVLLIGSDAGADRIGIRTDSMMVASIDTNGGATVLVGVPRNIQNFDFPEDN
                     PLSKVWPNGYNCGSECILTNAWMEAVNHKDLFPGDPNPGLTTTRGLVSEILGISIDYT
                     AVIDLRGFQDLVDAMGGVTIDIPKAVPIYGQGDVNPPISEWIKAGQNQHLDGYHALWY
                     ARSRKYDDDFHRMKRQRCVVGALVSEVDPVAMLSRYPAMARAIQKHVRVDIPLADLPA
                     WATLVERIQKGGMRSLALTPENISTVHPDLDSIHALVGKALTPSRQPTPASTSTSTKK
                     PAKTSTATPSANPTTAVDLDEAC"
     gene            complement(1246..1449)
                     /locus_tag="VHM65_11400"
     CDS             complement(1246..1449)
                     /locus_tag="VHM65_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX2706356.1"
                     /translation="MAQGTVKWFNAEKGFGFIAQDGGGPDVFVHFTAIQTNGYRSLDE
                     AQRVEFEVTQGPKGPQADAVRPL"
     gene            complement(1763..>2274)
                     /locus_tag="VHM65_11405"
     CDS             complement(1763..>2274)
                     /locus_tag="VHM65_11405"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX2706357.1"
                     /translation="GVAAEIGAGLVCSHTGGLPPRTDPVNTSYGPDRLDVVRDVLRTL
                     ARGARAAVTAGVPAERVLIDPTLDFGKTTTHSLTVLRHTADVVALGFPVLQAMSRKDF
                     VGESLDLPADERLEGSLAATAVAAWLGATVFRTHDVLATRRVVDMVASIRGDRLPAVA
                     VRGEPLHRR"
BASE COUNT          405 a          820 c          728 g          321 t
ORIGIN      
        1 gcctggcggt cgccgcaccg atcacgcagg cggtgcgcta tgcggcgatc tcgcgcgaca
       61 ccatgagcga gatcttcgtg gccacgtcga cggcggcccc gtcggtccgc cccgccgccg
      121 ccgaggttga cccctgggcc ggcacggccc gggtcagtgt gctcctcatc ggctccgatg
      181 ccggggccga ccggatcggg atccggaccg actcgatgat ggtggcctcg atcgacacca
      241 atggcggagc cacggtcctc gtcggcgtcc cccgcaacat ccagaacttc gacttccccg
      301 aagacaaccc cctgagcaag gtgtggccga acggctacaa ctgcggcagc gagtgcatcc
      361 tcaccaacgc ctggatggag gccgtgaacc acaaggacct ctttcccggt gaccccaacc
      421 ccggcctgac caccactcga gggctcgtct ccgagatcct cggcatcagc atcgactaca
      481 ccgccgtcat cgacctgaga ggattccagg acctcgtcga cgcgatgggg ggcgtgacca
      541 tcgacatccc caaggccgtc ccgatctacg ggcagggaga tgtcaaccca ccgatctcgg
      601 agtggatcaa agctggtcag aaccagcacc tcgacggcta ccacgcgctg tggtacgccc
      661 gctcgcgcaa gtacgacgac gacttccacc gcatgaagcg acagcgctgc gtcgtcggcg
      721 ccctggtgag cgaagtcgac ccggtggcca tgctgagcag gtacccggcc atggcccggg
      781 cgatccagaa acacgtccgc gtcgacatcc cgcttgccga cctaccggcg tgggccacgc
      841 tcgtggaacg catccagaag ggcggcatgc gcagcctggc gttgacgccc gagaacatct
      901 ccacggtcca ccctgacctg gactccatcc atgcgctcgt gggtaaggcg ctcacgccgt
      961 cgaggcagcc gacgccggcc tcgacgtcca cgtcgaccaa gaaaccggcg aagacctcca
     1021 cggccacgcc ctcggccaac cccaccacgg cggtcgacct ggacgaggcc tgctagcgcc
     1081 ccgggcgggt ccgccggcct ggcccaccgc agtccccaca acaccggact cgcgacaaca
     1141 ccgcagcccc gacgacaacg acagtggggc cctcggctga ccgggggccc cactgtctga
     1201 gaacgatgtc ctgctgtgat tcatccgccc ctcagggcgg aggggtcaga gcgggcgaac
     1261 agcgtcggcc tgcgggccct tggggccctg ggtgacctcg aactcgacgc gctgcgcctc
     1321 gtccagcgac cggtagccgt tcgtctggat cgcggtgaag tgcacgaaga cgtcggggcc
     1381 accgccgtcc tgagcgatga agccgaagcc cttctctgcg ttgaaccact tgacggtgcc
     1441 ttgtgccatg gggactactt tctgttctga gcctacggag cccgcacgtc acgggctccc
     1501 ggctgccgca ctacccccac accaactgga acatgcctgt cgtgctgaaa gaaaaggccc
     1561 gcgctgagga acagagcggg cggcttcttc gacctgcgag gaactgcaac tgcaaccgag
     1621 ggccatgtta tcagcaaacg gggtcaccgt aaccaccggg acccaaccga tctcatggct
     1681 tttgtggtct acccaaagac cgcattatgg ttgaacgagc aacaacgatc gggcactggg
     1741 cgtcataccg gcgcttcggg tgtcagcgcc ggtgcagggg ctcgccccgg accgcgactg
     1801 ccggaaggcg gtccccccgg atcgaggcca ccatgtccac cacgcgccgg gtggcgagca
     1861 cgtcgtgagt ccgaaagacc gttgcaccga gccacgccgc gaccgccgtc gccgcgagtg
     1921 agccctcgag ccgctcgtcg gcgggcaggt ccaggctctc accgacgaag tccttgcgag
     1981 acatcgcctg caggacgggg aaccccaggg ccacgacatc agccgtatgg cgcagcaccg
     2041 tcagcgagtg cgtggtcgtc ttgccgaagt cgagggtggg gtcgatcagg acccgctcgg
     2101 cgggcactcc cgccgtgacc gccgcacgag ccccccgagc cagcgtgcgc aggacgtcac
     2161 ggacgacgtc gagccggtcc ggaccgtagc tggtgttgac cgggtcggtg cgcgggggca
     2221 gccccccggt gtgcgagcag acgaggccgg cgccgatctc ggcggccacg ccgc
//
LOCUS       DATAIX010000770         1711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_147476, whole genome shotgun sequence.
ACCESSION   DATAIX010000770 DATAIX010000000
VERSION     DATAIX010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147476"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VHM65_11410"
     CDS             <1..209
                     /locus_tag="VHM65_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706358.1"
                     /translation="LVMRYKTTTLGTQFPDRWALPEALRDTTGVVLASAFPGYNPSSR
                     TSRPTSTTGPGASTSSPSKVSERT"
     gene            206..>1711
                     /locus_tag="VHM65_11415"
     CDS             206..>1711
                     /locus_tag="VHM65_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX2706359.1"
                     /translation="MSGAEPAVAEIDRLIAELRATLDAAPFEFDRRFLFRVLSMGHSQ
                     FAEIIGARGPNTQVNAACASTTQAISIAEDWIRSGRCLRVIVISGDDATGNGLLPWVS
                     SGFLASGAAATDERVEDAATPFDLRRHGMVVGAGSAAFVIESADAARERGIAPICEVL
                     GAVTANSAFHGTRLDLDHISGVMESVLSEAEQWGVDRHAIAPSLFFMSHETFTPARGG
                     SASAEINALRQSFGANADKIVITNTKGFTGHAMGAGIEDVVAIKALETGIVPPVPNYK
                     EPDPDLGTLNLSTGGSYPVQYALRLAAGFGSQIAMALLRWTPVPDGRHRSPSELGYSY
                     RVVDRGAWQRWIETISGHANGTLEVDHRRLRVVDTGAPTVPRTWVTAVPVPHALLGAV
                     GHAAAAPVAAAPSAPVAPAPAPAPAPARVAPAPAAAPAPVATAPAAAPAPVAAAAAPA
                     PVVSAPVAVSDPVMDVVTGIVAEMTGYPPDLLDPDLDLEADLGVDTVKQAEV"
BASE COUNT          229 a          647 c          572 g          263 t
ORIGIN      
        1 ccctcgtcat gcgctacaag acgacgacgc tgggcaccca gttccccgac cgctgggccc
       61 tgcccgaggc gctgcgggac acgaccggtg tcgtcttggc gtcggcgttc ccggggtaca
      121 acccctcgtc gaggacgtcg aggcctacgt cgacgaccgg gcccggcgcg agcacctcct
      181 cgccctcgaa ggtgtccgag cgcacatgag cggcgcggag ccggcggtgg ccgagatcga
      241 ccggctcatc gccgagctca gggccacact ggacgccgcg ccgttcgagt tcgaccggcg
      301 gttcctcttc cgggtcctgt cgatgggaca ctcgcagttc gccgagatca tcggcgcgcg
      361 gggcccgaac acccaggtca acgcagcctg cgcgagcacg acgcaggcca tctcgatcgc
      421 cgaggactgg atccgctccg gccgctgtct ccgggttatc gtcatctctg gcgacgacgc
      481 caccggtaac ggcttgttgc cgtgggtctc ctccggcttc ctggcctccg gcgccgctgc
      541 caccgacgag cgggtcgagg acgcggcaac gccgttcgac ctgcgccgcc acgggatggt
      601 cgtcggcgcc ggatcggccg ccttcgtcat cgagtccgcc gacgcggcac gagagcgtgg
      661 catcgcgccg atctgtgagg tcctcggggc agtcaccgct aacagcgcct tccacgggac
      721 tcgcctggac ctcgaccaca tcagtggcgt catggagtcc gtcctctccg aggccgagca
      781 gtggggcgtc gaccggcacg cgatcgcgcc gtcgctcttc ttcatgtcgc atgagacctt
      841 cacaccggct cggggcggca gcgcctcggc cgagatcaac gccctgcgcc agagcttcgg
      901 cgccaatgcc gacaagatcg tcatcaccaa caccaagggc ttcaccggtc acgcgatggg
      961 cgcgggcatc gaggacgtcg tcgcgatcaa ggcgctggag acgggcatcg tcccgccggt
     1021 ccccaactac aaggagcccg accccgacct cggcacactc aacctgtcca cgggtggctc
     1081 ctaccctgtg cagtatgccc tgcgtctggc ggccgggttc ggctcccaga tcgccatggc
     1141 cctgctgcgc tggacaccgg tgccggacgg gcgccaccgc tcgccgagcg agctcggcta
     1201 ctcctaccgg gtcgtcgacc ggggggcctg gcagcgttgg atcgagacga tctcgggcca
     1261 cgcgaacggc accctcgagg tggaccacag gcggttgcgg gtcgtcgata cgggcgcccc
     1321 gaccgtgccg aggacatggg tcaccgctgt cccggtcccg cacgcccttc tcggtgccgt
     1381 cgggcacgcg gcggctgctc cggtggccgc tgccccatca gcaccggtcg cgccggcacc
     1441 tgctccggca cctgctccgg ctcgggttgc tcctgctccc gccgctgcac cggctccggt
     1501 tgccacggct cccgccgccg cgcctgctcc tgtggctgcc gccgctgcgc ctgctccggt
     1561 cgtctcggcc ccggtggccg tgtccgaccc ggtcatggac gtcgtcacgg gcatcgtggc
     1621 cgagatgacg ggctacccgc cggacctgct cgacccggac ctggacctgg aggccgacct
     1681 cggggtcgac acggtcaagc aggccgaggt c
//
LOCUS       DATAIX010000771         2781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_267980, whole genome shotgun sequence.
ACCESSION   DATAIX010000771 DATAIX010000000
VERSION     DATAIX010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267980"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VHM65_11420"
     CDS             <1..753
                     /locus_tag="VHM65_11420"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX2706360.1"
                     /translation="QFSTSRGHVIYVRDVLERYGPDPLRYFICAAGPESQDSDFTWAE
                     FVQRNNSELVAGWGNLVNRTATMIHKSFGEIPAAGPLEVVDAELLATVRGAFGTVGAS
                     IERHRFKAGIGEVMRVVGEANAYVSKTAPFQLKGPDQRERLGTVLHVLAQAVSDINTL
                     MAPFLPHSANRVDAVLGGSGAFVPMPELREVEDLDGGPAYPVIMGDYTATPRWESRPV
                     VPGTLVGKPAPVFAKFDPSVVDEEIERMRGQD"
     gene            757..1623
                     /locus_tag="VHM65_11425"
     CDS             757..1623
                     /locus_tag="VHM65_11425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX2706361.1"
                     /translation="MTDPWDPRALPEPPRALGLPEPLPVPVVDNHAHLDLSREGEPLG
                     ELGAVIAAARSVGVTRLVQIGCDLDSARLTCDMVHSHPELLGGVALHPNEVPRLAAHG
                     LLPEAYEEIERLARDPRIRVVGETGLDYFRTGPEGAPMQQESFRWHIDLAKRLGLALQ
                     IHDREAHDDVLRILDEEGAPDFTVLHCFSGGVTMARAAVERGYLLSFAGTVTFKNAKA
                     LRDALAVTPLDQLLVETDAPYLTPSPHRGASNASYLLPWTVRRMADVLNVDVPTLCGR
                     ISATAERLYGPW"
     gene            1822..>2781
                     /locus_tag="VHM65_11430"
     CDS             1822..>2781
                     /locus_tag="VHM65_11430"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HEX2706362.1"
                     /translation="MAQAAVLSSVALGGIAITQADKVVTLTVDGATSTVHSFGSTVAD
                     LLDGQGITVGQHDLLIPGPDAPLSDGQSVALNYARKFTVNVDGSTTEFFTTARTVDAA
                     LGQLGIRTEGAELSVSRSLPLGRAGLAMTVTTPKTISILADGKTVDLVSTAGTVDEAL
                     REAGVTVGAHDRVTPVVTTALFGDSTVQVQRVRTASTAKTEKVAYSTTKKSDAALYKG
                     QSKTITKGVAGAKVVIYAETYVDGVLIVRRATSTHVVKTPVTAVVAVGTKPAPRPRPS
                     AGAVSGAGLNLANSAMWDRIASCESGGNWHINTGNGYYGGLQFL"
BASE COUNT          428 a          982 c          948 g          423 t
ORIGIN      
        1 cagttctcga cctcgcgcgg tcacgtcatc tacgtccggg acgtcctgga gcgctacggc
       61 cccgacccgt tgcgctactt catctgtgcg gcgggcccgg agagccagga cagtgacttc
      121 acctgggccg agttcgtcca gcgcaacaac tccgagctcg tcgccggctg gggcaacctc
      181 gtcaaccgga ccgcgacgat gatccacaag agcttcggcg agatcccggc ggccggcccg
      241 ctcgaggtgg tcgacgccga gctgctcgcc acggtgaggg gagccttcgg caccgtcggc
      301 gcgagcatcg agcggcaccg gttcaaggcc gggatcggcg aggtcatgcg ggtcgtcggc
      361 gaggccaacg cctatgtctc caagactgcg ccgttccagc tcaagggccc ggaccagcgg
      421 gagcgcctcg gcaccgtgct ccatgtcctg gcccaggccg tgtcggacat caacacgctc
      481 atggcgccgt tcctgccgca cagtgccaac cgggtcgatg ccgtcctcgg cggttcgggg
      541 gccttcgtcc cgatgcccga gctgcgggag gtcgaggacc tggacggggg cccggcatac
      601 ccggtcatca tgggcgacta cacggcgacc ccgcggtggg agtcccgccc ggtcgtcccg
      661 ggaaccctgg tcggcaagcc cgctccggtg ttcgccaagt tcgacccctc ggtcgtcgac
      721 gaggagatcg agcggatgcg cgggcaggac tagaccgtga ccgacccctg ggacccgcgc
      781 gctctccccg aacctccccg agccctcggg cttcccgagc cgctcccggt gcccgtggtc
      841 gacaaccacg cccacctgga cctgtcccgc gagggcgagc ccctcggtga gctgggggcg
      901 gtcatcgccg ctgcgcggtc ggtcggcgtc actcggctcg tccagatcgg ctgcgacctc
      961 gattcggctc gcctgacgtg tgatatggtt cacagccatc cggagctgct cgggggggtg
     1021 gcactgcacc cgaacgaggt cccccgcctg gctgcgcacg gcctgctccc cgaggcgtac
     1081 gaggagatcg agcgcctcgc gcgagacccg cggatccggg tcgtcggtga gacggggctg
     1141 gactacttcc ggaccgggcc cgagggggca ccgatgcagc aggagtcgtt ccggtggcac
     1201 atcgacctcg ccaagcggct ggggctggcg cttcagatcc acgaccggga ggcgcacgat
     1261 gacgtcctgc gcatcctcga cgaggaaggc gcgccggatt tcactgtgct gcattgcttt
     1321 tcgggtggcg tgacgatggc gagagcggcc gttgagcgcg gttacctcct gtcgttcgcg
     1381 ggcacggtga cgttcaagaa cgccaaggcg ctgcgggacg ctctggccgt caccccgctg
     1441 gaccagctgc tcgtcgagac ggatgcgccc tacctcaccc cgagcccaca tcgcggggcg
     1501 agcaatgcgt cgtatctcct gccgtggacg gtccgccgga tggccgatgt cctcaacgtc
     1561 gacgtgccga ccctgtgtgg ccggatctcg gcgacggcgg agcgcctcta cggtccgtgg
     1621 tgaggacccc ccgttggccc ggccgttacc aaaacttgac ctaagcagcg gcgtcggtca
     1681 tcgtggacgg acaagccggg tccctgatct tcccggcggc gacccgaaaa ccgctcctcc
     1741 ggagtgggct gggttccgcg cggtcagggc ctgggacgga cggcgagtgg agtgtgcgtg
     1801 gtttcccaga cagttcggcg tgtggcccag gcggcggtgc tcagcagtgt ggccctcggc
     1861 ggcattgcca tcacccaggc cgacaaggtc gtcaccctga cggtggacgg cgccaccagc
     1921 accgtgcatt cgttcggctc gacggtggcc gacctgctgg acggccaggg gatcaccgtg
     1981 ggtcagcacg acctgctcat tcccgggcca gacgcaccac tgtccgatgg ccagagcgtg
     2041 gcgctcaact acgcccgcaa gttcaccgtc aacgtggacg gctcgaccac ggagttcttc
     2101 acgaccgccc gcacggtcga cgctgcgctc ggccagctcg gaatccggac cgagggcgcc
     2161 gagctgtccg tctcgcgctc gctgccgctc gggcgcgccg gtctggcgat gaccgtcacc
     2221 accccgaaga cgatcagcat cctcgccgat ggcaagaccg tcgacctcgt cagcacggcg
     2281 ggcacggtcg acgaggcgct tcgcgaagcc ggagtcaccg tcggggccca tgaccgcgtc
     2341 actccggtcg tgacgacggc gctcttcggc gactcgacag tccaggtcca gcgggtccgc
     2401 acggcatcga cggccaagac cgagaaggtc gcctactcga cgacgaagaa gagcgacgct
     2461 gccctgtaca aaggtcagag caagaccatc accaaggggg tcgcgggcgc caaggtggtc
     2521 atctacgccg agacgtatgt cgacggcgtc ctcatcgttc gccgggctac gtccacccat
     2581 gtcgtcaaga cacccgtgac cgcggtcgtg gcggtcggga ccaagccagc cccgcggccc
     2641 aggcccagcg ccggggccgt gtccggcgcc gggctcaacc tggccaactc cgcgatgtgg
     2701 gaccgcatcg cgagctgcga gtccggcggc aactggcaca tcaataccgg aaacggctac
     2761 tacggcggcc tccagttcct c
//
LOCUS       DATAIX010000772         2068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_268016, whole genome shotgun sequence.
ACCESSION   DATAIX010000772 DATAIX010000000
VERSION     DATAIX010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268016"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VHM65_11435"
     CDS             complement(<1..301)
                     /locus_tag="VHM65_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706363.1"
                     /translation="MASNENENENDQTSTDTSTDTDTEGMLPGVGATVTPAQDGVGEG
                     AAGEAPAAAPAPARARRTRKTTARTRASTAAVPEATDQPAGDPALHAMVDAAEGDP"
     gene            complement(469..1218)
                     /locus_tag="VHM65_11440"
     CDS             complement(469..1218)
                     /locus_tag="VHM65_11440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HEX2706364.1"
                     /translation="MARQRVPEGPPPAPVVQKLRVRYAKRGRLRFSSSRDFQRALERA
                     LRRAEVPMAFSAGFHPHPRISYANAAPTGAASEAEYFEISLTRRVEPEVVRAALDQAL
                     PVGLDIVTVVEAEPGALADLLEASDWQLELAGLGAPEVQAAVGIFLEAGQTQVTRMMK
                     TGPRTFDVRPAVVQAMVIGSPEPNCAILRVVVRHTTPAVRPDDILTALRAVAGLEPPT
                     PPLVTRLAQGLLREGARLADPLVANESVDGT"
     gene            complement(1305..>2068)
                     /locus_tag="VHM65_11445"
     CDS             complement(1305..>2068)
                     /locus_tag="VHM65_11445"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2706365.1"
                     /translation="ADSLQPLDAAVVTDALLGNLRPEYRAMDVDPGNRFSVPWATGTT
                     AIGYDSSVLTEPPTWDVFLDATYAGRMTLLNERREAFAAALISLGKDPNTRDAATIAE
                     AEARLTRMAASSAFNSATYLDDLATGKVVVAQAFSTDVLQARKRNPKLAYTIPAAGAT
                     RWVDLLCIPADAPNAAAANAMIAYYLDPKVSATNAAYNLVATGNLKARDFVPKEVLDD
                     PAVYPPDDVVAELAFLVDLGETEQVYDEAWKRVKS"
BASE COUNT          277 a          715 c          756 g          320 t
ORIGIN      
        1 ccgggtcgcc ctcggcggcg tcgaccatgg cgtgcagggc ggggtcaccg gccggctggt
       61 cggtcgcctc cggtacggca gcggtggacg cccgagtcct cgcggttgtc ttccgcgtgc
      121 gtcgggcccg cgccggggcc ggggctgcgg ctggggcctc gccagcggcg ccctcaccga
      181 cgccgtcctg cgcgggggtc acggtcgcgc cgactccggg cagcatgccc tcggtgtcgg
      241 tgtcggtgct ggtgtcggtg ctggtctggt cgttctcgtt ctcgttctcg ttggaggcca
      301 tgaggcgtcc tctccgtcac ccacactggc gatgacccac acaggacctg tcggccgtca
      361 tgcccgcgtg gcgcggtcta cgtcgtccgg ctcgtgctgc tcacgggccg acggaagtcg
      421 tcctggtaca cctggcccag gctcgcgcag gccgcgcgtc accgcaggtc aggtgccgtc
      481 gacgctctcg ttggcaacca gagggtcagc caacctcgcg ccttcgcgga gcaggccctg
      541 agccagccga gtcaccagtg gtggcgtggg cggctcaagt ccggccacag cacgcagcgc
      601 ggtcagaatg tcgtcgggcc gaacggcagg tgtggtatgc cgaacaacca ctcggagtat
      661 cgcacagttc ggctcgggcg acccgatcac catcgcctgc acgacggcag gtcgcacgtc
      721 gaaggtgcgt ggacccgtct tcatcatccg ggtcacctgg gtctggccgg cctcgaggaa
      781 gatgccgacc gcagcctgca cctccggggc gccgagcccg gcgagctcga gctgccagtc
      841 actggcctcg agcaggtccg cgagcgcccc cggctcggcc tccaccaccg tgacgatgtc
      901 caggcccacg ggcagggcct ggtcgagggc ggcccgcacc acctcgggct cgacccggcg
      961 ggtgagcgag atctcgaagt actccgcctc actcgccgcg cccgtcgggg ccgcgttggc
     1021 atacgagatg cgcgggtggg gatggaaccc ggcgctgaac gccatgggaa cctcggcgcg
     1081 gcgcagggcc cgctccagag cgcgctggaa gtcccgcgac gacgagaagc gcaagcgccc
     1141 acgcttggca tagcgcaccc gcagcttctg caccacggga gccgggggcg gtccctcggg
     1201 gacgcgttga cgtgccatgc cgggcaacgc tactgcgccc gtcataccgg ccagggcacg
     1261 cgaacgaccc cgtcggcgaa accgcccgcc ataccggcgg ggtgtcagct cttgacgcgt
     1321 ttccacgcct cgtcgtagac ctgctcggtc tcgccgaggt cgacgaggaa cgcgagctcg
     1381 gcgacgacgt cgtcgggcgg gtagacggcc gggtcgtcga ggacctcctt gggtacgaag
     1441 tcgcgcgcct tgaggttgcc cgtcgccacg aggttgtatg ccgcgttggt cgccgagacc
     1501 ttggggtcga ggtagtaggc gatcatcgcg ttggcggccg ccgcgttcgg agcatccgcc
     1561 gggatgcaga gcaggtccac ccagcgtgtt gccccggcgg cggggatcgt gtaggccagc
     1621 ttggggttgc gcttgcgcgc ctgcaggacg tcggtgctga acgcctgggc cacgacgacc
     1681 ttgcccgtcg cgaggtcgtc gagataggtc gccgagttga acgccgacga cgccgccatt
     1741 ctcgtgagcc gcgcctcggc ctcggcgatc gtcgccgcgt cacgggtgtt ggggtccttg
     1801 cccagggaga tcagcgccgc ggcgaaggct tcgcgtcgct cgttgagcag ggtcatccga
     1861 ccggcgtacg tcgcgtcgag gaacacgtcc caggtcggcg gctcggtgag aaccgacgag
     1921 tcgtagccga tggcggtggt gccggtcgcc cagggcaccg agaaccggtt gcccgggtcg
     1981 acatccatgg cccggtactc cggccgcagg ttgcccagca gtgcgtcagt gacgacggcg
     2041 gcgtcgagcg gctgcagcga gtcggcct
//
LOCUS       DATAIX010000773         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_268081, whole genome shotgun sequence.
ACCESSION   DATAIX010000773 DATAIX010000000
VERSION     DATAIX010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268081"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VHM65_11450"
     CDS             complement(<1..883)
                     /locus_tag="VHM65_11450"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEX2706366.1"
                     /translation="MAAGTGRKLVIVESPAKAKTIASYLGGEYDVEASVGHIRDLPTP
                     SEMPAEMKKGPFGKFAIDVDNGFEPYYVVDADKRKKVAELKRYLKDADELYLATDEDR
                     EGEAIAWHLLETLKPRIPVKRMVFHEITREAIQRAVRETRELDTRLVDAQETRRILDR
                     LYGYEVSPVLWRKVRQGLSAGRVQSVAMRMVVERERERMAFRTASYWDVVAELTPHRD
                     TARAADTFTARLSSVDGHRVATGRDFGDDGVLRAGKTDTIARLDATAAQGIAEALHEA
                     VIRVTSVQEKPYTRRPSA"
     gene            1053..1841
                     /locus_tag="VHM65_11455"
     CDS             1053..1841
                     /locus_tag="VHM65_11455"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEX2706367.1"
                     /translation="MSDLRMPVLYLSHGAPPLADDPVWTRELAAWPGGIARPANILMI
                     SAHWEDAPLTVSSTTGAPLVYDFWGFPERYYTVRYDAPPAPALADEVSALLGGPAAGL
                     RRDESRGLDHGAYVPLVEMYPEADIPVLQMSMPSLDPAELFRIGAALAPLRDAGTLVV
                     GSGFTTHNLSLARFGEVGGWAPQWSREFDAWAAEVMAAGDVDALLDTMAKAPAADVAH
                     PRTEHWAPLYVALGAATAGSGALDSTSVIDGFWYGLSKRSWQFS"
BASE COUNT          288 a          730 c          699 g          339 t
ORIGIN      
        1 gggccgacgg ccggcgggtg tagggcttct cctggacgct cgtgacgcgg atcacggcct
       61 cgtgcagcgc ctcggcgatc ccctgggcgg cagtggcgtc gaggcgcgcg atggtgtcgg
      121 tcttgcccgc ccgcagcacc ccgtcgtcgc cgaagtcgcg cccggtggcg acacggtgcc
      181 cgtcaaccga gctgagccgg gcggtgaacg tgtccgcggc gcgggccgtg tcgcggtggg
      241 gggtcagctc ggccacgacg tcccagtagg acgcggtccg gaaggccatc cgctcgcgct
      301 cgcgctcgac gaccatccgc atcgcgacgg actggacgcg accggccgac agcccctggc
      361 ggaccttgcg ccacaggacg gggctgacct cgtagccgta gaggcggtcg aggatgcgcc
      421 gggtctcctg ggcgtcgacg aggcgggtgt cgagctcgcg cgtctcgcgg accgcacgct
      481 ggatcgcctc gcgggtgatc tcgtggaaca ccatgcgctt caccgggatt cgcggcttga
      541 gcgtctccag gagatgccag gcaatggcct cgccctcgcg gtcctcgtcg gtcgcgaggt
      601 agagctcgtc cgcgtccttg aggtaacgct tgagctcggc gaccttcttg cgcttgtcgg
      661 catcgacgac gtagtagggc tcgaacccgt tgtcgacatc gatcgcgaac ttgccgaacg
      721 ggcccttctt catctccgcg ggcatctccg acggggtcgg caggtcccgg atatggccga
      781 ccgacgcctc gacgtcatac tcgcccccga gataggaggc gatcgtcttc gccttggccg
      841 gcgactcgac gatgacgagc ttgcgccccg tgcctgcggc cacgacaccc ctcgcattcc
      901 tggccccacg tgcagcggat tcgtcgctgc tcggacgtga cgagccggag cacaccgtat
      961 gacatgtcgg tgtgagttcg cataccgggc tgggcccacc cgcgcgcgcc gcgatgtcgt
     1021 tgaaacttgc actactgagt agactggtcc tcatgagcga cctgcggatg ccagttctct
     1081 acctcagcca cggagccccg ccgctcgcgg acgaccccgt gtggacccgc gagctggccg
     1141 cgtggccggg cgggatcgcc cggccggcga acattctcat gatctccgcg cactgggagg
     1201 acgccccgct cacggtctcc tcgaccactg gcgctcccct cgtctacgac ttctgggggt
     1261 tcccggagcg ctactacacc gtccggtatg acgcacctcc ggccccagcg ctcgcggacg
     1321 aggtctcggc gctcctcggc ggtcccgcgg ccggcctgcg ccgggacgag agccgcggtc
     1381 tggaccacgg ggcctacgtg ccactggtcg agatgtaccc cgaggccgac atcccggtcc
     1441 tgcagatgtc gatgccctcg ctcgacccgg ccgagctgtt ccgcatcggg gcggccctgg
     1501 ctccgctgcg cgacgccggg acgctcgtcg tcgggtcggg cttcacgacg cacaacctct
     1561 cgctcgcccg gttcggtgag gtcgggggct gggcgccgca gtggtcgcgc gagttcgacg
     1621 cctgggccgc cgaggtgatg gccgcagggg acgtcgacgc cctgctcgac acgatggcca
     1681 aggctcccgc cgccgacgtc gcccacccca ggaccgagca ctgggccccc ttgtatgtcg
     1741 ccctcggcgc cgccactgca gggtccgggg cgctggacag cacgagcgtc atcgacggct
     1801 tctggtacgg cctgtccaag cgctcctggc agttcagctg acgtctgagg cagttcaggc
     1861 tgacgtgtga ggcagttcag ctgacgtctg aggcagctga cgtctgacac agtgcgcgcc
     1921 atggacatca agacattgac cgacgaggtc gagaacatct ccctcatcta cgcctcgcgc
     1981 ttcgagatcg accgcaccga cacgtggttc ctgctcaagc ttcaggagga ggtcggcgaa
     2041 ctgacccagg cccacc
//
LOCUS       DATAIX010000774         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_148378, whole genome shotgun sequence.
ACCESSION   DATAIX010000774 DATAIX010000000
VERSION     DATAIX010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148378"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..>2103
                     /gene="dnaK"
                     /locus_tag="VHM65_11460"
     CDS             287..>2103
                     /gene="dnaK"
                     /locus_tag="VHM65_11460"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX2706368.1"
                     /translation="MARAVGIDLGTTNSAVAVLEGGEPSIIANAEGGRTTPSVVAFSK
                     NGEVLVGEIAKRQAVTNVDRTIRSVKRHMGTDWKSGDIDGRAYTAQEISARVLMKLKR
                     DAESYLGETVTDAVITVPAYFDDHQRQATKEAGEIAGLNVLRIINEPTAAALAYGLDK
                     GKEDELILVFDLGGGTFDVSLLEVGKDPEDGFSTIQVRATNGDNHLGGDDWDERIVNH
                     LLTTVKNSSGTDLSKDKIAMQRLRDAAEQAKKELSTATTTNISMQYLSMSENGPIHLD
                     ESLSRAQFEQLTTDLLERTKAPFQKVIQDAGIKLSEIDHVVLVGGSTRMPAVTSVVRE
                     LTGGREPNKGVNPDEVVAVGASLQAGVLKGERKDVLLIDVTPLSLGIETQGGRFTKLI
                     ERNTAIPTKRSEIFTTAEDNQPSVLIQVYQGEREIASHNKGLGTFELTGIAPAPRNVP
                     KIEVTFDIDANGIVHVSAKDQGTGKEQSMTISGGSALPKDEIERMVREAEAHAEEDKV
                     RVAEIETRNTAEQLVYSTEKFVSENDEKLPADGKEKVTSAILDLKRALDGTDVEDVKA
                     KHEALAKASQELGSALYAASAEASDGGPGAAAGSSAGSSA"
BASE COUNT          419 a          731 c          687 g          266 t
ORIGIN      
        1 ggcggaggag ccctcttcag tcgactgcga ccagggccgc gcgacgggca gcggtgcggg
       61 ccgggtgcgg gaacattgcg cggcatccga gagttgagtc gactgtacgc aactttgcgc
      121 agtcctggct tgacaaccga gtcggtatga cgcaagttga gtctggacgt atcaaccccg
      181 actaggccgg gtccggcagg ccgtcgcacg agagcgacgg tgagccggtc cggcataccg
      241 gatcgtgagt cacccactca cggcagagca acaggagaga accaccatgg cacgtgcggt
      301 cggcatcgac ctcggtacca ccaactcggc cgtcgccgtc ctcgagggcg gcgagccgag
      361 catcatcgcg aacgcggagg gcggccggac cacccccagc gtcgtcgcct tcagcaagaa
      421 cggcgaggtg ctcgtcggcg agattgccaa gcgccaggcc gtcaccaacg tcgaccggac
      481 catccgctcg gtcaagcgcc acatgggcac cgactggaag tccggcgaca tcgacggcag
      541 ggcctacacc gcccaggaga tcagcgcccg cgtgctcatg aagctcaagc gtgacgcgga
      601 gtcctacctc ggcgagacgg tgaccgacgc ggtcatcacg gtcccggcct acttcgacga
      661 ccaccagcgc caggcgacca aggaggccgg cgagatcgcg ggcctcaacg tcctgcggat
      721 catcaacgag ccgaccgcgg cggccctggc ctatggcctc gacaagggca aggaggacga
      781 gctcatcctc gtcttcgacc tcggtggcgg cacgttcgac gtgtccctcc tggaggtcgg
      841 caaggacccc gaggacggct tctcgacgat ccaggtccgc gcgaccaacg gtgacaacca
      901 cctcggcggc gacgactggg acgagcgcat cgtcaaccac ctgctcacca cggtgaagaa
      961 ctcctccggc accgacctgt ccaaggacaa gatcgccatg cagcgtctgc gcgacgcggc
     1021 cgagcaggcc aagaaggagc tctcgaccgc cacgacgacc aacatctcca tgcagtacct
     1081 ctcgatgtcc gagaacggcc cgatccacct cgacgagtcg ctgagccgcg cccagttcga
     1141 gcagctgacg accgacctgc tcgagcgcac caaggcgccc ttccagaagg tcatccagga
     1201 tgccggcatc aagctgtccg agatcgacca cgtcgtcctc gtcggcggct cgacccggat
     1261 gcctgccgtg acgagcgtcg tcagggagct caccggcggc cgcgagccca acaagggcgt
     1321 caacccggac gaggtcgtcg ccgtcggcgc gagcctgcag gccggggtgc tcaagggtga
     1381 gcgcaaggac gtcctgctca tcgacgtcac cccgctgtcg ctcggcatcg agacccaggg
     1441 cgggcgcttc accaagctca tcgagcgcaa caccgcgatc ccgaccaagc gctcggagat
     1501 cttcacgacg gccgaggaca accagccttc ggtgctgatc caggtctacc agggcgagcg
     1561 cgaaatcgcc tcgcacaaca agggactcgg cacgttcgag ctcaccggca tcgctccggc
     1621 cccgcgcaac gtgcccaaga tcgaggtcac cttcgacatc gacgccaacg gcatcgtgca
     1681 cgtgtccgcc aaggaccagg gcacgggcaa ggagcagtcg atgacgatct ccggcgggtc
     1741 cgccctgccc aaggacgaga tcgagcgcat ggtccgcgag gccgaggcgc acgccgagga
     1801 ggacaaggtg cgcgtcgcgg agatcgagac ccgcaacacc gccgagcagc tcgtctactc
     1861 gaccgagaag ttcgtctccg agaacgacga gaagctgccc gccgacggca aggagaaggt
     1921 gaccagcgcg atcctcgacc tgaagcgggc gctggacggc accgacgtcg aagacgtcaa
     1981 ggccaagcac gaggcactcg ccaaggccag ccaggagctc ggctccgcgc tgtacgccgc
     2041 ttcggccgag gcgtcggacg gcggcccggg tgcagctgca gggtcgtcag cagggtcgtc
     2101 agc
//
LOCUS       DATAIX010000775         1562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_148383, whole genome shotgun sequence.
ACCESSION   DATAIX010000775 DATAIX010000000
VERSION     DATAIX010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148383"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VHM65_11465"
     CDS             <1..236
                     /locus_tag="VHM65_11465"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEX2706369.1"
                     /translation="NSSGAKSCLGVVDGNATKGVTWVHSYVTPDREKTFCVYDGPDPE
                     AIKAAAEVTGLPVDSITEVRVLDPYFYLNETGS"
     gene            complement(217..396)
                     /locus_tag="VHM65_11470"
     CDS             complement(217..396)
                     /locus_tag="VHM65_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2706370.1"
                     /translation="MSSTYGPHETFPAACPQTKVIAEAIRDLRTVEVDQAIGNPAQTK
                     VAAIPTSAALKTPFR"
     gene            complement(431..1036)
                     /locus_tag="VHM65_11475"
     CDS             complement(431..1036)
                     /locus_tag="VHM65_11475"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2706371.1"
                     /translation="MDSGEWDGRYSEHEQMWSGRSNGTLLVEVGGLAPGTALDVGCGE
                     GGDAVWLAERGWRVTGLDVSRVALARAQANSRARNVDVEWVCAALADLEPPAVGYDLV
                     SVHYPALQHAPGNDAIHALLTAVGQGGTLLVVGHARIDPEHAQAHGFDPADYVQPRDV
                     AAQLDDGWVVEVDEERPRVDPSPVGTEHVYDHVLRARRSRA"
BASE COUNT          226 a          557 c          535 g          244 t
ORIGIN      
        1 tgaacagcag cggcgccaag tcctgtctcg gcgtcgttga cggcaacgcg accaagggcg
       61 tgacgtgggt gcactcgtac gtgacacccg accgcgagaa gacgttctgc gtgtatgacg
      121 gtcccgaccc cgaggcgatc aaggccgccg ccgaggtcac cggtctgccc gtggactcga
      181 tcaccgaggt gcgggtgctc gacccgtact tctacctcaa cgaaacgggg tcttgagggc
      241 ggcactggtc gggatcgcgg cgaccttggt ctgcgcgggg ttgccgatgg cctggtcgac
      301 ctcgacggtc ctgagatctc ggatcgcctc ggcgatgacc ttcgtttgcg ggcacgccgc
      361 tgggaaggtc tcatggggtc cgtacgtcga gctcaccgac tctgcgggca cggcgagtgg
      421 aagggtcggc tcaggctcgg gatcgtcgcg cccgcagcac gtggtcgtag acgtgctcgg
      481 tgcctacggg tgacgggtcg acccggggac gctcctcgtc gacctccacc acccagccgt
      541 cgtcaagctg tgcggcgacg tcccgcggct ggacgtagtc ggccgggtcg aacccgtggg
      601 cctgcgcgtg ctcagggtcg atgcgcgcat ggcccaccac gagcagagtg ccgccctgcc
      661 cgacggcggt cagcagggcg tgaatcgcgt cgttcccggg ggcgtgctgc agcgccgggt
      721 agtgcacgct gaccaggtcg tagccgaccg ccggcggttc gaggtcggcc agcgctgcac
      781 acacccactc gacgtcgacg ttccgggccc gcgaattggc ctgcgcccga gccagcgcca
      841 cccgcgagac gtccaatccg gtgacccgcc agccgcgctc cgccaaccag acggcgtccc
      901 cgccctcgcc gcacccgacg tccagcgcgg tgcccggcgc cagccccccg acctcgacca
      961 gcagcgtccc gttggaacgc ccgctccaca tctgctcgtg ctcggagtac cgcccgtccc
     1021 actcgccact gtccacgggg ccatcctgcc gcagtccgcc cggcggaccg accatctgcg
     1081 tttctcaacc gccgacagcg cgctcacgcg cgaccatgcc gctctcggga gcgagcgtcg
     1141 agcagttcgt gccgaggtgc gtgcgccgac cggattgtca gggattcctt cgaccggcca
     1201 tcgcgtcgcc gccgccgaaa gcccttcctg gacagtgtcc tcagcgtcat cgaattcgcc
     1261 gaagcgccgt ttgaggaccg cgaggacgtc aggcgccagg tgcctcagga gctcctcgat
     1321 gcccggagac ggtgtcacgg ctccaggtcg ggtcggaccg tcacgggccg cacctggatc
     1381 tctcgggccg tggacgcccc ccgccggggc cgggcggcga agcggcgctt caggacctta
     1441 ggtcgccaga ggccagtgcg ggtgctttgg tgcgcgatcc tggaaagtca agattgcagg
     1501 attctggatg accccgtcac ggatctcgat ggcccgacga atggtggggt ccgcgtccca
     1561 gg
//
LOCUS       DATAIX010000776         1958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Lustribacter sp. isolate SMAG_U6895
            k141_148810, whole genome shotgun sequence.
ACCESSION   DATAIX010000776 DATAIX010000000
VERSION     DATAIX010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955513
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Lustribacter sp. (soil metagenome)
  ORGANISM  Candidatus Lustribacter sp.
            Bacteria; Vulcanimicrobiota; Vulcanimicrobiia; Vulcanimicrobiales;
            Vulcanimicrobiaceae; Candidatus Lustribacter.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,278
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Candidatus Lustribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148810"
                     /isolate="SMAG_U6895"
                     /isolation_source="soil"
                     /db_xref="taxon:3069335"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VHM65_11480"
     CDS             complement(<1..639)
                     /locus_tag="VHM65_11480"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX2706372.1"
                     /translation="MTSIRALVVPRHGDASVLTVEDRELAPPGAGEVQVAVAAAGVNF
                     IDIYQREGVYPVPTPFVLGMEGAGTVTAVGPDVQGMTGGDRVAWAFALGSAATAANVS
                     AAAVAPVPDGIGLDTAAAAMLQGMTAHYLTRSTYAVREGDTVLVHAAAGGVGQLLVQM
                     CTRLGARVIATAGSSAKLDIARRLGADELVDYSAVTDVAGEVRRLTDGRGVEV"
     gene            complement(650..1183)
                     /locus_tag="VHM65_11485"
     CDS             complement(650..1183)
                     /locus_tag="VHM65_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2706373.1"
                     /translation="MSPAESPDRPPVVLPSGAVLRAAIPADVPELLRLVVELATFEKE
                     PDSVLACVADYEAILFPGHGAPTGHAEVAQLDGRLVGMAFWYVTFSTWSGHNGIWLED
                     LYVEPGHRGLGVGTAMLRRLAGICAARGYHRLEWWVLDWNEPAIRFYRGLGARALDGW
                     THYRIDGDALAQTGGPS"
     gene            complement(1266..1589)
                     /locus_tag="VHM65_11490"
     CDS             complement(1266..1589)
                     /locus_tag="VHM65_11490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2706374.1"
                     /translation="MPSTNRWLVGGSEFLGHLSIRHRLTPVLVELGGHIGYAIRPSAR
                     RRGYGTRQLALALPVARGLGLGRLLVTCDEDNIGSARIIERSGGVLEDVRGDKRRYWI
                     DVPGR"
BASE COUNT          284 a          683 c          729 g          262 t
ORIGIN      
        1 gacctcgacc ccgcgcccgt cggtgagccg ccggacctcg cccgcgacgt cggtgaccgc
       61 gctgtagtcg acgagctcgt cggcgccgag gcggcgtgcg atgtcgagct tggcgctcga
      121 tccggccgtc gcgatgaccc gcgcgccgag cctggtgcac atctggacga gcagctgccc
      181 gaccccaccg gctgcggcgt ggacgaggac cgtatctcct tctcgcaccg cgtaggtcga
      241 gcgcgtcagg tagtgcgcgg tcatcccctg gagcatcgcg gccgcggcgg tgtcgaggcc
      301 gatcccgtcg ggcacggggg cgaccgcggc ggcgctcacg ttggccgcag tggccgcgct
      361 gcccagggcg aaggcccacg cgacgcggtc accgcccgtc ataccctgga cgtctggtcc
      421 caccgcggtg accgtgccgg cgccctccat cccgaggacg aacggcgtcg ggaccgggta
      481 gacgccttcg cgctggtaga tgtcgatgaa gttgaccccg gcggcggcga ccgccacctg
      541 gacctcaccc gccccgggcg gggcgagctc gcggtcctcg acggtgagca ccgacgcatc
      601 gccgtggcgg gggacgacca gggcgcggat cgacgtcata ctgcccagcc tacgaggggc
      661 caccggtctg cgcgagggcg tcgccgtcga tgcggtagtg agtccacccg tccagggccc
      721 gggcgccgag gccccgatag aagcggatcg cgggctcgtt ccagtccagg acccaccact
      781 cgagccggtg gtagccgcgc gcggcgcaga tgccggcgag ccgccgcagc atcgccgtgc
      841 cgaccccgag cccgcgatgg ccgggctcga cgtagaggtc ctccagccag atcccgttgt
      901 gaccactcca ggtcgagaag gtgacgtacc agaaggccat ccccacgagg cgcccgtcga
      961 gctgggcgac ctcggcatgc ccggtcggcg ccccgtggcc ggggaagagg atcgcctcgt
     1021 agtcggcgac gcacgcgagc accgagtcgg gctccttctc gaaggtcgcc agctcgacga
     1081 cgaggcgcag cagctcgggg acgtcggccg gtatggcggc ccgcaggacc gcgcccgaag
     1141 gcaggacgac gggaggccgg tcgggtgact cagcggggga catggcgcga atgctagcca
     1201 acggtggcac ggcccggacg ttgcgggtct tccgggtcca gcagggttgc cgggcagggc
     1261 ccggctcacc gacccggcac gtcgatccag tagcgccgct tgtcgccccg aacgtcctcg
     1321 aggaccccac cggagcgctc gatgatccgg gccgacccga tgttgtcctc gtcgcacgtg
     1381 acgaggagcc ggccaaggcc gagaccccgg gccaccggca gggccagcgc gagctggcgg
     1441 gtgccgtatc cgcggcggcg cgctgacggg cggatcgcgt agccgatgtg tccgccgagc
     1501 tcgaccagca cgggagtgag acggtgccgg atcgagaggt ggccgaggaa ctcactgccc
     1561 ccgaccagcc agcggttcgt cgagggcacc cggccgggcg ggcgcgggtg gtctccatcg
     1621 cctcggcgat ctccagctcg acgtgggcgg tgaagacgct ggggtcatcg agggcggtcc
     1681 gcgcgaggcg gatgtcgcgg ccggtggcct gggcgatgtc acgcagaccg ctggagcgcg
     1741 cctgaccggc ggcgatgtac tcgtcgagag cggccaggta ggaccgctgg tagcgcacct
     1801 tcgggatggc gagctccacc acgagtccac cgtatggcgc tgcgccccct cggtctcgcg
     1861 ggttcccggt ccattacatg aacgtcaggt atccataatc tgggattatt tgacgatggg
     1921 ctattcggaa ggggcgccga cgcgtcatgc tcgtgatg
//
